AR084757A1 - PROCEDURE FOR IMMOBILIZATION OF NUCLEIC LIGANDS - Google Patents

PROCEDURE FOR IMMOBILIZATION OF NUCLEIC LIGANDS

Info

Publication number
AR084757A1
AR084757A1 ARP110105039A ARP110105039A AR084757A1 AR 084757 A1 AR084757 A1 AR 084757A1 AR P110105039 A ARP110105039 A AR P110105039A AR P110105039 A ARP110105039 A AR P110105039A AR 084757 A1 AR084757 A1 AR 084757A1
Authority
AR
Argentina
Prior art keywords
target molecule
support
complex
nucleic
immobilization
Prior art date
Application number
ARP110105039A
Other languages
Spanish (es)
Inventor
Gerald Perret
Egisto Boscheti
Original Assignee
Lfb Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1061366A external-priority patent/FR2970003B1/en
Priority claimed from FR1159604A external-priority patent/FR2981651B1/en
Application filed by Lfb Biotechnologies filed Critical Lfb Biotechnologies
Publication of AR084757A1 publication Critical patent/AR084757A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3278—Polymers being grafted on the carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Reivindicación 1: Procedimiento de inmovilización de ligandos nucleicos que comprenden, al menos, una función amina primaria, por injerto sobre un soporte sólido que presenta grupos ácidos carboxílicos activados, que comprenden una etapa de acoplamiento covalente de dichos ácidos nucleicos sobre dicho soporte sólido a un pH inferior a 6.Reivindicación 13: Método de preparación de un soporte de afinidad que comprende la implementación del procedimiento de inmovilización tal como se ha definido en una cualquiera de las reivindicaciones 1 a 12. Reivindicación 19: Complejo resultante de la interacción de un ligando nucleico y una molécula diana, donde dicho complejo está formado en la superficie de un soporte sólido tal como se ha definido en una cualquiera de las reivindicaciones 14 a 18. Reivindicación 20: Utilización de un soporte de afinidad tal como se ha definido en una cualquiera de las reivindicaciones 14 a 18 para la purificación de una molécula diana. Reivindicación 22: Procedimiento de purificación de una molécula diana con un soporte de afinidad tal como se ha definido de acuerdo con una cualquiera de las reivindicaciones 14 a 18, que comprende las etapas siguientes: a) poner en contacto una composición por purificar que comprende la molécula diana de interés con un soporte sólido de afinidad tal como se ha definido en una de las reivindicaciones 14 a 18, con el objeto de formar un complejo entre (i) ligandos nucleicos injertados sobre dicho soporte y (ii) dicha molécula diana, y b) liberar dicha molécula diana a partir del complejo formado en la etapa a), y recuperar dicha molécula diana purificada.Claim 1: Method of immobilization of nucleic ligands comprising at least one primary amine function, by grafting on a solid support having activated carboxylic acid groups, comprising a step of covalent coupling of said nucleic acids on said solid support to a solid support. pH lower than 6. Claim 13: Method of preparation of an affinity support comprising the implementation of the immobilization process as defined in any one of claims 1 to 12. Claim 19: Complex resulting from the interaction of a ligand nucleic and a target molecule, wherein said complex is formed on the surface of a solid support as defined in any one of claims 14 to 18. Claim 20: Use of an affinity support as defined in any one of claims 14 to 18 for the purification of a target molecule. Claim 22: Purification process of a target molecule with an affinity support as defined according to any one of claims 14 to 18, comprising the following steps: a) contacting a composition to be purified comprising the target molecule of interest with a solid affinity support as defined in one of claims 14 to 18, in order to form a complex between (i) nucleic ligands grafted onto said support and (ii) said target molecule, and b ) releasing said target molecule from the complex formed in step a), and recovering said purified target molecule.

ARP110105039A 2010-12-30 2011-12-30 PROCEDURE FOR IMMOBILIZATION OF NUCLEIC LIGANDS AR084757A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1061366A FR2970003B1 (en) 2010-12-30 2010-12-30 METHOD FOR IMMOBILIZATION OF NUCLEIC ACIDS
FR1159604A FR2981651B1 (en) 2011-10-24 2011-10-24 METHOD FOR IMMOBILIZATION OF NUCLEIC LIGANDS

Publications (1)

Publication Number Publication Date
AR084757A1 true AR084757A1 (en) 2013-06-05

Family

ID=45531495

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110105039A AR084757A1 (en) 2010-12-30 2011-12-30 PROCEDURE FOR IMMOBILIZATION OF NUCLEIC LIGANDS

Country Status (10)

Country Link
US (1) US9803184B2 (en)
EP (1) EP2658864A1 (en)
JP (1) JP6178727B2 (en)
KR (1) KR20140057187A (en)
CN (1) CN103403015A (en)
AR (1) AR084757A1 (en)
AU (1) AU2011350672B2 (en)
BR (1) BR112013016704A2 (en)
CA (1) CA2823030A1 (en)
WO (1) WO2012090183A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2823030A1 (en) 2010-12-30 2012-07-05 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Method for immobilising nucleic ligands
FR3011250A1 (en) 2013-09-30 2015-04-03 Lab Francais Du Fractionnement NUCLEIC ACIDS BINDING SPECIFICALLY TO HUMAN IX / IXA FACTOR, AND USES
FR3021330A1 (en) 2014-05-20 2015-11-27 Lab Francais Du Fractionnement METHOD FOR ASSAYING DNA APTAMERS
GB201503578D0 (en) * 2015-03-03 2015-04-15 Ge Healthcare Bio Sciences Ab Sanitization method for affinity chromatography matrices
PT109480B (en) * 2016-06-22 2020-03-13 Inst Superior Tecnico POLYMENZIMIDAZOLE POLYMER WITH FUNCTIONED SPACER CHAIN AND ITS METHOD OF OBTAINING TO REMOVE GENOToxic IMPURITIES
EP4293116A3 (en) 2016-07-06 2024-01-24 Laboratoire Français du Fractionnement et des Biotechnologies Stable liquid fibrinogen
US20190256853A1 (en) * 2016-07-28 2019-08-22 Laboratoire Francais Du Fractionnement Et Des Biot Echnologies Method for Obtaining Aptamers
CA3031390A1 (en) 2016-07-28 2018-02-01 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Anti- immunoglobulin g aptamers and uses thereof
CN106512958B (en) * 2016-11-22 2019-03-08 华南师范大学 A kind of preparation method and application of aptamer modified chitosan nano fiber
WO2018109213A1 (en) 2016-12-16 2018-06-21 Laboratoire Français Du Fractionnement Et Des Biotechnologies Aptamers directed against a kappa light chain-containing protein and uses thereof
GB201714563D0 (en) * 2017-09-11 2017-10-25 Life Tech As Coupling methods and compositions
WO2020023841A1 (en) * 2018-07-27 2020-01-30 Massachusetts Institute Of Technology Oligomerized protein-polymer conjugates
GB2580384B (en) * 2019-01-08 2021-01-27 Quantumdx Group Ltd Oligonucleotide deposition onto polypropylene substrates
CN109806852B (en) * 2019-03-04 2020-08-07 北京理工大学 Application of adenine functionalized polypropylene glycol
WO2023084369A1 (en) * 2021-11-12 2023-05-19 3M Innovative Properties Company Method of processing polynucleic acids

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CA2823030A1 (en) 2010-12-30 2012-07-05 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Method for immobilising nucleic ligands

Also Published As

Publication number Publication date
JP6178727B2 (en) 2017-08-09
EP2658864A1 (en) 2013-11-06
US20130344567A1 (en) 2013-12-26
US9803184B2 (en) 2017-10-31
CA2823030A1 (en) 2012-07-05
AU2011350672B2 (en) 2016-06-02
CN103403015A (en) 2013-11-20
AU2011350672A1 (en) 2013-05-02
KR20140057187A (en) 2014-05-12
WO2012090183A1 (en) 2012-07-05
JP2014505867A (en) 2014-03-06
BR112013016704A2 (en) 2017-03-14

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