AR082928A1 - METHODS TO DETERMINE THE PRESENCE OR CIGOSITY OF SOY EVENT OF AAD-12 1606 - Google Patents

METHODS TO DETERMINE THE PRESENCE OR CIGOSITY OF SOY EVENT OF AAD-12 1606

Info

Publication number
AR082928A1
AR082928A1 ARP110103279A ARP110103279A AR082928A1 AR 082928 A1 AR082928 A1 AR 082928A1 AR P110103279 A ARP110103279 A AR P110103279A AR P110103279 A ARP110103279 A AR P110103279A AR 082928 A1 AR082928 A1 AR 082928A1
Authority
AR
Argentina
Prior art keywords
event
present application
assays
aad
soybean
Prior art date
Application number
ARP110103279A
Other languages
Spanish (es)
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR082928A1 publication Critical patent/AR082928A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Adicionalmente, la presente solicitud proporciona ensayos para detectar la presencia del evento de soja pDAB4472-1606 (Evento 1 606) como está presente en una semilla depositada en la Colección Americana de cultivo, bajo el nº de acceso PTA-11028. En algunas realizaciones, los ensayos se pueden basar en la secuencia de ADN del constructo recombinante, insertado en el genoma de soja, y en las secuencias genómicas que flanquean el sitio de inserción. La presente solicitud también se relaciona en parte con los ensayos de PCR TaqMan como criterio de valoración del Evento 1606 que expresa AAD-12 en soja. Algunas realizaciones se dirigen a ensayos que son capaces de un análisis de cigosidad de alto rendimiento. Estos y otros procedimientos relacionados pueden utilizarse para identificar de manera exclusiva la cigosidad de líneas de soja de mejora que comprende el evento de la presente solicitud. Los kits y condiciones útiles para conducir estos ensayos también se proporcionan.Additionally, the present application provides tests to detect the presence of the soybean event pDAB4472-1606 (Event 1 606) as it is present in a seed deposited in the American Culture Collection, under accession number PTA-11028. In some embodiments, the assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences that flank the insertion site. The present application also relates in part to the TaqMan PCR assays as an evaluation criterion of Event 1606 expressed by AAD-12 in soybeans. Some embodiments are directed to assays that are capable of a high performance cygosity analysis. These and other related procedures can be used to uniquely identify the cygosity of improvement soybean lines that comprise the event of the present application. The kits and conditions useful for conducting these tests are also provided.

ARP110103279A 2010-09-08 2011-09-08 METHODS TO DETERMINE THE PRESENCE OR CIGOSITY OF SOY EVENT OF AAD-12 1606 AR082928A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US38095210P 2010-09-08 2010-09-08

Publications (1)

Publication Number Publication Date
AR082928A1 true AR082928A1 (en) 2013-01-16

Family

ID=45811138

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110103279A AR082928A1 (en) 2010-09-08 2011-09-08 METHODS TO DETERMINE THE PRESENCE OR CIGOSITY OF SOY EVENT OF AAD-12 1606

Country Status (2)

Country Link
AR (1) AR082928A1 (en)
WO (1) WO2012033808A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI607090B (en) * 2011-10-18 2017-12-01 陶氏農業科學公司 Materials and methods for detecting the aryloxyalkanoate dioxygenase gene (aad-12) in plants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0225129D0 (en) * 2002-10-29 2002-12-11 Syngenta Participations Ag Improvements in or relating to organic compounds
US8088976B2 (en) * 2005-02-24 2012-01-03 Monsanto Technology Llc Methods for genetic control of plant pest infestation and compositions thereof
BRPI0618025B1 (en) * 2005-10-28 2016-12-27 Dow Agrosciences Llc method for controlling weeds in an area, isolated polynucleotide, plant cell and herbicide resistant plant

Also Published As

Publication number Publication date
WO2012033808A3 (en) 2014-03-20
WO2012033808A2 (en) 2012-03-15

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