AR075403A1 - SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION - Google Patents

SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION

Info

Publication number
AR075403A1
AR075403A1 ARP100100388A AR075403A1 AR 075403 A1 AR075403 A1 AR 075403A1 AR P100100388 A ARP100100388 A AR P100100388A AR 075403 A1 AR075403 A1 AR 075403A1
Authority
AR
Argentina
Prior art keywords
sequence
vector
synthetic
boina
nucleotid
Prior art date
Application number
Other languages
Spanish (es)
Inventor
Hugo Menzella
Original Assignee
Geneg S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneg S R L filed Critical Geneg S R L
Priority to ARP100100388 priority Critical patent/AR075403A1/en
Priority to PCT/ES2011/070089 priority patent/WO2011098651A1/en
Publication of AR075403A1 publication Critical patent/AR075403A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Abstract

Una secuencia de nucleotidos sintética que codifica para proquimosina A recombinante bovina seleccionada del conjunto comprendido por SEQID Ns 2, SEQID Ns 3, SEQID Ns 4, SEQID Ns 5, SEQID Ns 6. Un vector de expresion que comprende la secuencia de nucleotidos sintética de la presente invencion. Una célula de Escherichia coli transgénica que comprende dicha secuencia de nucleotidos sintética y un proceso para producir proquimosina A con dicha célula.A synthetic nucleotide sequence encoding recombinant bovine prochymosin A selected from the set comprised of SEQID Ns 2, SEQID Ns 3, SEQID Ns 4, SEQID Ns 5, SEQID Ns 6. An expression vector comprising the synthetic nucleotide sequence of the present invention. A transgenic Escherichia coli cell comprising said synthetic nucleotide sequence and a process for producing prochymosin A with said cell.

ARP100100388 2010-02-11 2010-02-11 SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION AR075403A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ARP100100388 AR075403A1 (en) 2010-02-11 2010-02-11 SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION
PCT/ES2011/070089 WO2011098651A1 (en) 2010-02-11 2011-02-11 Synthetic sequence of nucleotides coding for bovine recombinant prochymosin a, expression vector comprising said sequence, escherichia coli cell converted by means of said vector, and the process of the obtainment of said bovine recombinant prochymosin a

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP100100388 AR075403A1 (en) 2010-02-11 2010-02-11 SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION

Publications (1)

Publication Number Publication Date
AR075403A1 true AR075403A1 (en) 2011-03-30

Family

ID=43857549

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100100388 AR075403A1 (en) 2010-02-11 2010-02-11 SEQUENCE OF SYNTHETIC NUCLEOTID THAT CODIFIES FOR BOVINE RECOMBINANT, EXPRESSION VECTOR THAT INCLUDES SUCH SEQUENCE, TRANSFORMED COLOR ESCHERICHIA CELL WITH SUCH VECTOR AND PROCESSING PROCEDURE FOR BOINA RECOMBINATION

Country Status (2)

Country Link
AR (1) AR075403A1 (en)
WO (1) WO2011098651A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68797A0 (en) * 1982-07-01 1983-09-30 Genex Corp Cloned bovine calf chymosin and calf prochymosin genes,their preparation,plasmids containing them and microorganisms transformed by those plasmids
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
EP1231272A3 (en) * 2001-02-08 2002-11-13 Laboratorios Ovejero S.A. Procedure for obtaining bovine chymosin (rennin), curd

Also Published As

Publication number Publication date
WO2011098651A1 (en) 2011-08-18

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