AR059366A1 - PROCEDURE AND APPARATUS FOR THE REMOVAL AND RECOVERY OF CELLS THAT ADHER TO A SOLID SUBSTRATE - Google Patents

PROCEDURE AND APPARATUS FOR THE REMOVAL AND RECOVERY OF CELLS THAT ADHER TO A SOLID SUBSTRATE

Info

Publication number
AR059366A1
AR059366A1 ARP070100523A ARP070100523A AR059366A1 AR 059366 A1 AR059366 A1 AR 059366A1 AR P070100523 A ARP070100523 A AR P070100523A AR P070100523 A ARP070100523 A AR P070100523A AR 059366 A1 AR059366 A1 AR 059366A1
Authority
AR
Argentina
Prior art keywords
substrate
cells
attraction
solid substrate
adher
Prior art date
Application number
ARP070100523A
Other languages
Spanish (es)
Inventor
Gianmarco Roggero
Original Assignee
Consul T S S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consul T S S R L filed Critical Consul T S S R L
Publication of AR059366A1 publication Critical patent/AR059366A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Proceso y aparato para quitar y recuperar las células que adhieren a un substrato solido en un envase de cultivo de células que contiene el substrato y un medio fluido en contacto con el substrato. El procedimiento comprende las operaciones de introducir una cantidad susceptible de partículas de material de atraccion magnética en el medio fluido, generando un campo magnético de atraccion que actua en el material de partículas dicho, y variando el dicho campo de atraccion de tal manera en cuanto genere un flujo del material de partículas dicho que pasta el substrato y capaz de causar la separacion de adherir las células del substrato. El aparato incluye un miembro movil con una pluralidad de imanes permanentes sobre su superficie enfrentando el substrato e impartiendo el medio motor el movimiento rotatorio y el movimiento de la revolucion sobre el miembro movil.Process and apparatus for removing and recovering the cells that adhere to a solid substrate in a cell culture container containing the substrate and a fluid medium in contact with the substrate. The method comprises the operations of introducing a susceptible amount of particles of magnetic attraction material into the fluid medium, generating a magnetic field of attraction that acts on the said particle material, and varying said attraction field in such a way as it generates a flow of the said particulate material that grazes the substrate and capable of causing the separation of adhering the cells of the substrate. The apparatus includes a mobile member with a plurality of permanent magnets on its surface facing the substrate and imparting the rotary movement and the movement of the revolution on the mobile member.

ARP070100523A 2006-02-10 2007-02-08 PROCEDURE AND APPARATUS FOR THE REMOVAL AND RECOVERY OF CELLS THAT ADHER TO A SOLID SUBSTRATE AR059366A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITTO20060095 ITTO20060095A1 (en) 2006-02-10 2006-02-10 PROCEDURE AND EQUIPMENT FOR THE REMOVAL AND RECOVERY OF ADHESIVE CELLS TO A SOLID SUBSTRATE

Publications (1)

Publication Number Publication Date
AR059366A1 true AR059366A1 (en) 2008-03-26

Family

ID=38051767

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP070100523A AR059366A1 (en) 2006-02-10 2007-02-08 PROCEDURE AND APPARATUS FOR THE REMOVAL AND RECOVERY OF CELLS THAT ADHER TO A SOLID SUBSTRATE

Country Status (3)

Country Link
AR (1) AR059366A1 (en)
IT (1) ITTO20060095A1 (en)
WO (1) WO2007091198A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4219411A (en) * 1978-09-18 1980-08-26 California Institute Of Technology Cell sorting apparatus
DE19644761C1 (en) * 1996-10-29 1998-02-05 Heraeus Instr Gmbh Cell culturing and harvesting apparatus
US6054319A (en) * 1998-02-03 2000-04-25 Board Of Trustees Operating Michigan State University Method and apparatus for growing cells using gas or liquid aphrons
EP1291413A1 (en) * 2001-09-07 2003-03-12 The Automation Partnership (Cambridge) Limited Cell culture harvesting

Also Published As

Publication number Publication date
ITTO20060095A1 (en) 2007-08-11
WO2007091198A1 (en) 2007-08-16

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