AR038137A1 - COMPOSITION THAT INCLUDES A MODIFIED LESQUERELLA FATTY ACID HYDROXYLASE POLYPEPTIDE, RECOMBINATING DNA MOLECULA, EXPRESSION VECTOR AND ORGANISM TRANSFORMED WITH THE RECOMBINANT DNA MOLECULA; PLANT, PLANT CELL AND TRANSFORMED PLANT SEED; OIL OBTAINED FROM SUCH SEED; - Google Patents

COMPOSITION THAT INCLUDES A MODIFIED LESQUERELLA FATTY ACID HYDROXYLASE POLYPEPTIDE, RECOMBINATING DNA MOLECULA, EXPRESSION VECTOR AND ORGANISM TRANSFORMED WITH THE RECOMBINANT DNA MOLECULA; PLANT, PLANT CELL AND TRANSFORMED PLANT SEED; OIL OBTAINED FROM SUCH SEED;

Info

Publication number
AR038137A1
AR038137A1 ARP030100094A AR038137A1 AR 038137 A1 AR038137 A1 AR 038137A1 AR P030100094 A ARP030100094 A AR P030100094A AR 038137 A1 AR038137 A1 AR 038137A1
Authority
AR
Argentina
Prior art keywords
plant
transformed
seed
modified
fatty acid
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Brookhaven Science Ass Llc
Shanklin John N
Broadwater John A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brookhaven Science Ass Llc, Shanklin John N, Broadwater John A filed Critical Brookhaven Science Ass Llc
Publication of AR038137A1 publication Critical patent/AR038137A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Composición que comprende un polipéptido de hidroxilasa de ácido graso lesquerella modificado, que comprende un aminoácido no activo en la posición 149, en la posición 325 o ambas posiciones, donde los aminoácidos en las posiciones 63, 105, 149, 218,296, 323 y 325 no más de tres son aminoácidos no nativos, y donde una especificidad de reacción de la hidroxilasa modificada difiere de una especificidad de reacción de una hidroxilasa modificada difiere de una especificidad de reacción de una hidroxilasa no modificada. Molécula de ADN recombinante que comprende la secuencia de ácido nucleico correspondiente operablemente unida a al menos una secuencia reguladora adecuada, vector de expresión que comprende la molécula de ADN recombinante y organismo transformado con dicha molécula. El organismo se puede seleccionar de microorganismos y plantas. Planta, célula de planta y semilla de planta transformada, y aceite obtenida de dicha semilla. Célula de levadura transformada, célula bacteriana transformada y aceite obtenido de la misma. Método para producir una hidroxilasa modificada, método para alterar el fenotipo de una planta y método para evaluar la desaturación del ácido graso o la actividad de hidroxilación de una enzima.Composition comprising a modified lesquerella fatty acid hydroxylase polypeptide, comprising a non-active amino acid at position 149, at position 325 or both positions, where amino acids at positions 63, 105, 149, 218,296, 323 and 325 no more than three are non-native amino acids, and where a reaction specificity of the modified hydroxylase differs from a reaction specificity of a modified hydroxylase differs from a reaction specificity of an unmodified hydroxylase. Recombinant DNA molecule comprising the corresponding nucleic acid sequence operably linked to at least one suitable regulatory sequence, expression vector comprising the recombinant DNA molecule and organism transformed with said molecule. The organism can be selected from microorganisms and plants. Plant, plant cell and transformed plant seed, and oil obtained from said seed. Transformed yeast cell, transformed bacterial cell and oil obtained from it. Method to produce a modified hydroxylase, method to alter the phenotype of a plant and method to evaluate the desaturation of fatty acid or the hydroxylation activity of an enzyme.

ARP030100094 2002-01-14 2003-01-14 COMPOSITION THAT INCLUDES A MODIFIED LESQUERELLA FATTY ACID HYDROXYLASE POLYPEPTIDE, RECOMBINATING DNA MOLECULA, EXPRESSION VECTOR AND ORGANISM TRANSFORMED WITH THE RECOMBINANT DNA MOLECULA; PLANT, PLANT CELL AND TRANSFORMED PLANT SEED; OIL OBTAINED FROM SUCH SEED; AR038137A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34855702P 2002-01-14 2002-01-14

Publications (1)

Publication Number Publication Date
AR038137A1 true AR038137A1 (en) 2004-12-29

Family

ID=23368536

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030100094 AR038137A1 (en) 2002-01-14 2003-01-14 COMPOSITION THAT INCLUDES A MODIFIED LESQUERELLA FATTY ACID HYDROXYLASE POLYPEPTIDE, RECOMBINATING DNA MOLECULA, EXPRESSION VECTOR AND ORGANISM TRANSFORMED WITH THE RECOMBINANT DNA MOLECULA; PLANT, PLANT CELL AND TRANSFORMED PLANT SEED; OIL OBTAINED FROM SUCH SEED;

Country Status (3)

Country Link
AR (1) AR038137A1 (en)
AU (1) AU2003235589A1 (en)
WO (1) WO2003060092A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0407296A (en) 2003-02-05 2006-03-07 Divergence Inc nucleic acids encoding anthelmintic agents and plants produced from them
US7368629B2 (en) 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
UA118090C2 (en) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
CN108753803B (en) * 2018-07-16 2019-04-23 河北省农林科学院粮油作物研究所 A kind of high oleic acid peanut mutated gene AhFAD2B-814 and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6310194B1 (en) * 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases

Also Published As

Publication number Publication date
WO2003060092A3 (en) 2004-02-19
AU2003235589A8 (en) 2003-07-30
WO2003060092A2 (en) 2003-07-24
AU2003235589A1 (en) 2003-07-30

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