AR030390A1 - ISOFLAVONOID ENZYME FOR METHODATION - Google Patents

ISOFLAVONOID ENZYME FOR METHODATION

Info

Publication number
AR030390A1
AR030390A1 ARP000102477A ARP000102477A AR030390A1 AR 030390 A1 AR030390 A1 AR 030390A1 AR P000102477 A ARP000102477 A AR P000102477A AR P000102477 A ARP000102477 A AR P000102477A AR 030390 A1 AR030390 A1 AR 030390A1
Authority
AR
Argentina
Prior art keywords
isoflavonoid
methylated
iomt
plants
accumulation
Prior art date
Application number
ARP000102477A
Other languages
Spanish (es)
Inventor
Richard A Dixon
Xian Z He
Original Assignee
Samuel Roberts Noble Found Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samuel Roberts Noble Found Inc filed Critical Samuel Roberts Noble Found Inc
Publication of AR030390A1 publication Critical patent/AR030390A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
    • A61P5/30—Oestrogens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Diabetes (AREA)
  • Oncology (AREA)
  • Endocrinology (AREA)
  • Communicable Diseases (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Se describen métodos para manipular genéticamente isoflavonoides 4'-O-metilados biologicamente activos que se han encontrado con base en el carácter regioespecífico de 7-OMT de isoflavona in vivo. Después de la transformacion y expresion de un gen de O-metiltransferasa de isoflavonoide, la sobre-regulacion de IOMT en las plantas transgénicas puede ser utilizada para elevar la acumulacion de fitoalexinas de isoflavonoide 4'-O-metiladas, proveyendo una resistencia de enfermedad incrementada a la planta. Se pueden utilizar métodos similares para incrementar la acumulacion de nutracéuticos de isoflavonoide 4' ûO- metilado en plantas. Para la sub-regulacion de IOMT en plantas que naturalmente hacen fitoalexinas 4-O-isoflavonoide y nutracéuticos de isoflavonoide 4'-O-metilados, las secuencias de gen IOMT pueden ser transformadas en la orientacion antisentido.Methods for genetically manipulating biologically active 4'-O-methylated isoflavonoids that have been found based on the regiospecific character of isoflavone 7-OMT in vivo are described. After transformation and expression of an isoflavonoid O-methyltransferase gene, over-regulation of IOMT in transgenic plants can be used to increase the accumulation of 4'-O-methylated isoflavonoid phytoalexins, providing increased disease resistance. to the plant. Similar methods can be used to increase the accumulation of 4 'û-methylated isoflavonoid nutraceuticals in plants. For the sub-regulation of IOMT in plants that naturally make 4-O-isoflavonoid phytoalexins and 4'-O-methylated isoflavonoid nutraceuticals, the IOMT gene sequences can be transformed into the antisense orientation.

ARP000102477A 1999-05-20 2000-05-22 ISOFLAVONOID ENZYME FOR METHODATION AR030390A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13502699P 1999-05-20 1999-05-20

Publications (1)

Publication Number Publication Date
AR030390A1 true AR030390A1 (en) 2003-08-20

Family

ID=22466165

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000102477A AR030390A1 (en) 1999-05-20 2000-05-22 ISOFLAVONOID ENZYME FOR METHODATION

Country Status (6)

Country Link
EP (1) EP1183376A1 (en)
JP (1) JP2004500803A (en)
AR (1) AR030390A1 (en)
AU (1) AU780464B2 (en)
CA (1) CA2373751A1 (en)
WO (1) WO2000071736A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100946771B1 (en) 2003-09-19 2010-03-11 건국대학교 산학협력단 Naringenin 7-O-methyltransferase and method for preparing Sakuranetin using the gene
WO2008022265A2 (en) * 2006-08-16 2008-02-21 The Samuel Roberts Noble Foundation, Inc. Plant isoflavone and isoflavanone o-methyltransferase genes
US20150290271A1 (en) * 2010-07-12 2015-10-15 Frutarom Switzerland Ltd. Nutraceutical Composition Obtained from Fungus-Challenged Soy Seedlings
BR112021011352A2 (en) * 2018-12-14 2021-09-08 Basf Se EXPRESSION CONSTRUCTION, VECTOR, PLANT OR PLANT CELL, HARVEST PART OF A PLANT, METHODS FOR CHECKING, INCREASE OR MODIFYING RESISTANCE IN A PLANT AND FOR PRODUCTION OF A TRANSGENIC PLANT, USE OF AN EXPRESSION CONSTRUCTION, PRODUCT OF A PLANT, METHODS OF IMPROVING A PLANT AND IDENTIFICATION OF AN AGENT AND COATED PLANT

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704400A (en) * 1986-06-24 1987-11-03 Merck & Co., Inc. Medicarpin derivatives and analogs
CA2136233C (en) * 1992-05-19 2004-03-30 Graham Edmund Kelly Health supplements containing phyto-oestrogens, analogues or metabolites thereof
WO2000037652A2 (en) * 1998-12-21 2000-06-29 E.I. Du Pont De Nemours And Company Flavonoid biosynthetic enzymes

Also Published As

Publication number Publication date
CA2373751A1 (en) 2000-11-30
EP1183376A1 (en) 2002-03-06
AU5018700A (en) 2000-12-12
JP2004500803A (en) 2004-01-15
WO2000071736A1 (en) 2000-11-30
AU780464B2 (en) 2005-03-24

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