AR020947A1 - OPTIMIZED GENES FOR EXPRESSION IN PLANTS THAT CODIFY PESTICIDE TOXINS OF THE CYTC TYPE - Google Patents

OPTIMIZED GENES FOR EXPRESSION IN PLANTS THAT CODIFY PESTICIDE TOXINS OF THE CYTC TYPE

Info

Publication number
AR020947A1
AR020947A1 ARP990105354A ARP990105354A AR020947A1 AR 020947 A1 AR020947 A1 AR 020947A1 AR P990105354 A ARP990105354 A AR P990105354A AR P990105354 A ARP990105354 A AR P990105354A AR 020947 A1 AR020947 A1 AR 020947A1
Authority
AR
Argentina
Prior art keywords
expression
plants
optimized
present
cytc
Prior art date
Application number
ARP990105354A
Other languages
Spanish (es)
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Publication of AR020947A1 publication Critical patent/AR020947A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

La presente invencion se refiere a materiales y métodos utiles para el control de plagas y, en especial, de plagas de las plantas. Más específicamente, lapresente invencion presenta nuevas secuencias de polinucleotidos optimizadas para la expresion en plantas que codifican toxinas pesticidas. Las secuencias depolinucleotidos de la presente invencion presentan ciertas modificaciones, en comparacion con las secuencias de tipo salvaje, por ejemplo que las hacenespecialmente adecuadas para la expresion optimizada en plantas. Mediante el uso de las secuencias de polinucleotidos aquí descriptas, se puede lograr latransformacion de plantas, por medio de técnicas conocidos por los expertos en la técnica, para conferir a dichas plantas resistencia alas plagas. En lasrealizaciones preferidas, la presente invencion presenta secuencias de polinucleotidos optimizadas para la expresion en plantas que codifican proteínas toxicasa las que se puede denominar toxinas cytC. También se describe una toxina preferida codificada por un gen optimizado para la expresion en plantas.The present invention relates to useful materials and methods for the control of pests and, especially, of plant pests. More specifically, the present invention presents new polynucleotide sequences optimized for expression in plants encoding pesticidal toxins. The depolinucleotide sequences of the present invention have certain modifications, compared to wild-type sequences, for example that make them especially suitable for optimized expression in plants. By using the polynucleotide sequences described herein, plant transformation can be achieved, by means of techniques known to those skilled in the art, to confer such resistance to pests. In preferred embodiments, the present invention features optimized polynucleotide sequences for expression in plants encoding toxicase proteins which may be referred to as cytC toxins. A preferred toxin encoded by a gene optimized for plant expression is also described.

ARP990105354A 1998-10-23 1999-10-22 OPTIMIZED GENES FOR EXPRESSION IN PLANTS THAT CODIFY PESTICIDE TOXINS OF THE CYTC TYPE AR020947A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10540398P 1998-10-23 1998-10-23

Publications (1)

Publication Number Publication Date
AR020947A1 true AR020947A1 (en) 2002-06-05

Family

ID=22305640

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990105354A AR020947A1 (en) 1998-10-23 1999-10-22 OPTIMIZED GENES FOR EXPRESSION IN PLANTS THAT CODIFY PESTICIDE TOXINS OF THE CYTC TYPE

Country Status (3)

Country Link
AR (1) AR020947A1 (en)
AU (1) AU1218200A (en)
WO (1) WO2000024905A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995002694A2 (en) * 1993-07-15 1995-01-26 Mycogen Corporation Materials and methods for the control of calliphoridae pests

Also Published As

Publication number Publication date
WO2000024905A1 (en) 2000-05-04
AU1218200A (en) 2000-05-15

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