AR020945A1 - OPTIMIZED POLINUCLEOTIDES FOR EXPRESSION IN PLANTS CODING PESTICIDE PROTEINS TYPE 43F AND 80JJ1 / 130 - Google Patents

OPTIMIZED POLINUCLEOTIDES FOR EXPRESSION IN PLANTS CODING PESTICIDE PROTEINS TYPE 43F AND 80JJ1 / 130

Info

Publication number
AR020945A1
AR020945A1 ARP990105352A ARP990105352A AR020945A1 AR 020945 A1 AR020945 A1 AR 020945A1 AR P990105352 A ARP990105352 A AR P990105352A AR P990105352 A ARP990105352 A AR P990105352A AR 020945 A1 AR020945 A1 AR 020945A1
Authority
AR
Argentina
Prior art keywords
expression
present
optimized
polynucleotide sequences
toxins
Prior art date
Application number
ARP990105352A
Other languages
Spanish (es)
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Publication of AR020945A1 publication Critical patent/AR020945A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Catching Or Destruction (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invencion se refiere a materiales y métodos utiles para el control de plagas y, en especial, de plagas de las plantas. Más específicamente lapresente invencion presenta nuevas secuencias de polinucleotidos optimizadas para la expresion enplantas que codifican toxinas pesticidas. Las secuencias depolinucleotidos de la presente invencion presentan ciertas modificaciones, en comparacion con las secuencias del tipo salvaje, que las que las hacenespecialmente adecuadas para la expresionoptimizadas para la expresion en plantas. Mediante el uso de las secuencias de polinucleotidos aquí descriptas, sepuede lograr la transformacion de plantas, por medio de técnicas conocidos por los expertos en la técnica, para conferir a dichas plantas resistencia a lasplagas. Las secuencias de polinucleotidos de la presente invencion también pueden ser truncadas para producir toxinas truncadas y para producir genes yproteínas híbridos o quiméricos (de fusion). En una realizacion preferida, la presente invencion presenta secuencias de polinucleotidos optimizadas para laexpresion en plantas que codifican una toxina de longitud total de aproximadamente 130 kDa, a la que se puede denominar proteína tipo 80JJ1/130. En otrarealizacion preferida,la presente invencion presenta secuencias de polinucleotidos optimizadas para la expresion en plantas que codifican toxinas proteicas alas que se puede denominar toxinas tipo 43F.The present invention relates to useful materials and methods for the control of pests and, especially, of plant pests. More specifically, the present invention presents new polynucleotide sequences optimized for expression in plants encoding pesticidal toxins. The depolinucleotide sequences of the present invention have certain modifications, compared to wild-type sequences, which make them especially suitable for optimized expression for plant expression. By using the polynucleotide sequences described herein, it is possible to achieve plant transformation, by means of techniques known to those skilled in the art, to confer such resistance to pests. The polynucleotide sequences of the present invention can also be truncated to produce truncated toxins and to produce hybrid or chimeric (fusion) genes and proteins. In a preferred embodiment, the present invention features polynucleotide sequences optimized for expression in plants encoding a total length toxin of approximately 130 kDa, which may be referred to as 80JJ1 / 130 type protein. In another preferred embodiment, the present invention features polynucleotide sequences optimized for expression in plants encoding protein toxins wings that can be termed type 43F toxins.

ARP990105352A 1998-10-23 1999-10-22 OPTIMIZED POLINUCLEOTIDES FOR EXPRESSION IN PLANTS CODING PESTICIDE PROTEINS TYPE 43F AND 80JJ1 / 130 AR020945A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10545598P 1998-10-23 1998-10-23
US10545298P 1998-10-23 1998-10-23

Publications (1)

Publication Number Publication Date
AR020945A1 true AR020945A1 (en) 2002-06-05

Family

ID=26802598

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990105352A AR020945A1 (en) 1998-10-23 1999-10-22 OPTIMIZED POLINUCLEOTIDES FOR EXPRESSION IN PLANTS CODING PESTICIDE PROTEINS TYPE 43F AND 80JJ1 / 130

Country Status (3)

Country Link
AR (1) AR020945A1 (en)
AU (1) AU1448500A (en)
WO (1) WO2000024903A2 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ230375A (en) * 1988-09-09 1991-07-26 Lubrizol Genetics Inc Synthetic gene encoding b. thuringiensis insecticidal protein
AU5987794A (en) * 1992-12-31 1994-08-15 Mycogen Corporation Novel (bacillus thuringiensis) toxins active against corn rootworm larvae

Also Published As

Publication number Publication date
WO2000024903A3 (en) 2000-08-31
AU1448500A (en) 2000-05-15
WO2000024903A2 (en) 2000-05-04

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