AR016229A1 - USEFUL GENES TO INCREASE PROTEIN CONTENT OF PLANTS, PROTEINS CODED BY SUCH GENES, CELLS OF PLANTS TRANSFECTED WITH SUCH GENES AND METHODS TO INCREASE PROTEIN OR NON-PROTEIC CONTENT OF PLANTS - Google Patents

USEFUL GENES TO INCREASE PROTEIN CONTENT OF PLANTS, PROTEINS CODED BY SUCH GENES, CELLS OF PLANTS TRANSFECTED WITH SUCH GENES AND METHODS TO INCREASE PROTEIN OR NON-PROTEIC CONTENT OF PLANTS

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Publication number
AR016229A1
AR016229A1 ARP990101938A ARP990101938A AR016229A1 AR 016229 A1 AR016229 A1 AR 016229A1 AR P990101938 A ARP990101938 A AR P990101938A AR P990101938 A ARP990101938 A AR P990101938A AR 016229 A1 AR016229 A1 AR 016229A1
Authority
AR
Argentina
Prior art keywords
protein
equal
plants
gene
genes
Prior art date
Application number
ARP990101938A
Other languages
Spanish (es)
Original Assignee
Demegen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Demegen Inc filed Critical Demegen Inc
Publication of AR016229A1 publication Critical patent/AR016229A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un gen que codifica una proteína seleccionada del grupo que consiste en )i) una proteína que comprende una secuencia alfa-helicoidal anfifílica donde dichasecuencia comprende (H)u((h)x(H)y)z(h)w donde H es un residuo de aminoácido hidrofílico y puedevariar a lo largo de dicha proteína, h es un residuo deaminoácido hidrofobico y puede variar a lo largo de dicha proteína, u es igual a 0 o 1, x es igual a 1 o 2, y es igual a 1 o 2, z está seleccionado del grupoque consiste en 5,6,7,8,9,10,11,12,13,14,15,16, 17, 18, 19, 20, y cualquier numero entero superior a 20, y w es igual a 0,1 o 2, y donde el valor de x en un((h)x(H)y) no necesita ser igual al valor de x en otro ((h)x(H)y) y donde el valor de y en un ((h)x(H)y) no necesita ser igual al valor de y en otro ((h)x(H)y)y (ii) una proteína que comprende una secuencia de una lámina beta-plegada donde dicha secuencia comprende (H)r(hH)s(h)t donde H es un residuo de aminoácidohidrofílico y puede variar a lo largo de dicha proteína, h es un residuo de aminoácido hidrofobico y puede variar a lo largo de dicha proteína r, es igual a 0o 1, s es cualquier numero entero superior a 0 y t es igual a 0,1 o 2. La proteína codificada es una proteína disenada de novo, de almacenamiento artificial hasido expresada establemente en plantas.Esta proteína fue disenada para poseer elevados niveles de todos los aminoácidos esenciales necesarios para la nutricionhumana. La expresion del gen que codifica para esta proteína en plantas de cultivo mejorará enormemente la calidad nutricional de los cultivos resultantes. Seha observado también que el gen aumenta el nivel general de produccion de proteínas en una planta. Esta propiedad permite mejorar los niveles de produccion deotras proteínas valiosasen una planta. Por ejemplo, una planta transgénica con un gen que codifica para la insulina puede producir niveles más elevados deinsulina cuando la planta expresa también un gen para una proteína de almacenamiento artificial. El método permite también mejorar la produccion de losproductos no proteicos. La co-transformacion con un gen de interés puede dar como resultado niveles incrementados del producto proteico del gen, de interés oA gene encoding a protein selected from the group consisting of) i) a protein comprising an amphiphilic alpha-helical sequence where dichasequence comprises (H) or ((h) x (H) y) z (h) w where H is a hydrophilic amino acid residue and may vary along said protein, h is a hydrophobic amino acid residue and may vary along said protein, or is equal to 0 or 1, x is equal to 1 or 2, and is equal to 1 or 2, z is selected from the group consisting of 5,6,7,8,9,10,11,12,13,14,15,16, 17, 18, 19, 20, and any integer greater than 20 , yw is equal to 0.1 or 2, and where the value of x in one ((h) x (H) y) does not need to be equal to the value of x in another ((h) x (H) y) y where the value of y in one ((h) x (H) y) does not need to be equal to the value of y in another ((h) x (H) y) and (ii) a protein comprising a sequence of a sheet beta-folded where said sequence comprises (H) r (hH) s (h) t where H is a hydrophilic amino acid residue and may vary along said protein na, h is a hydrophobic amino acid residue and may vary along said protein r, is equal to 0 or 1, s is any integer greater than 0 and t is equal to 0.1 or 2. The encoded protein is a protein de novo, artificial storage has been stably expressed in plants. This protein was designed to have high levels of all the essential amino acids necessary for human nutrition. The expression of the gene that codes for this protein in crop plants will greatly improve the nutritional quality of the resulting cultures. It has also been observed that the gene increases the general level of protein production in a plant. This property allows to improve the production levels of other valuable proteins in a plant. For example, a transgenic plant with a gene coding for insulin can produce higher levels of insulin when the plant also expresses a gene for an artificial storage protein. The method also allows to improve the production of non-protein products. Co-transformation with a gene of interest may result in increased levels of the protein product of the gene, of interest or

ARP990101938A 1998-04-27 1999-04-27 USEFUL GENES TO INCREASE PROTEIN CONTENT OF PLANTS, PROTEINS CODED BY SUCH GENES, CELLS OF PLANTS TRANSFECTED WITH SUCH GENES AND METHODS TO INCREASE PROTEIN OR NON-PROTEIC CONTENT OF PLANTS AR016229A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6605698A 1998-04-27 1998-04-27

Publications (1)

Publication Number Publication Date
AR016229A1 true AR016229A1 (en) 2001-06-20

Family

ID=22066979

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990101938A AR016229A1 (en) 1998-04-27 1999-04-27 USEFUL GENES TO INCREASE PROTEIN CONTENT OF PLANTS, PROTEINS CODED BY SUCH GENES, CELLS OF PLANTS TRANSFECTED WITH SUCH GENES AND METHODS TO INCREASE PROTEIN OR NON-PROTEIC CONTENT OF PLANTS

Country Status (4)

Country Link
AR (1) AR016229A1 (en)
AU (1) AU3868199A (en)
CA (1) CA2325463A1 (en)
WO (1) WO1999055890A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0411479A (en) * 2003-06-17 2006-07-25 Sembiosys Genetics Inc methods for producing insulin in plants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989004371A1 (en) * 1987-11-02 1989-05-18 Louisiana State University Agricultural And Mechan Plants genetically enhanced for disease resistance
US5559223A (en) * 1991-08-09 1996-09-24 E. I. Dupont De Nemours And Company Synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants
FR2744134B1 (en) * 1996-01-29 1998-04-03 Biocem RESERVE PROTEINS FROM PLANTS ENRICHED IN AMINO ACIDS, IN PARTICULAR ALPHA-ZEIN BUT ENRICHED IN LYSINE PLANTS EXPRESSING SUCH PROTEINS

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WO1999055890A1 (en) 1999-11-04
AU3868199A (en) 1999-11-16
CA2325463A1 (en) 1999-11-04

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