AR015757A1 - ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF REGULATORY PROTEINS OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER DIFFERENT METHOD - Google Patents

ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF REGULATORY PROTEINS OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER DIFFERENT METHOD

Info

Publication number
AR015757A1
AR015757A1 ARP990101634A ARP990101634A AR015757A1 AR 015757 A1 AR015757 A1 AR 015757A1 AR P990101634 A ARP990101634 A AR P990101634A AR P990101634 A ARP990101634 A AR P990101634A AR 015757 A1 AR015757 A1 AR 015757A1
Authority
AR
Argentina
Prior art keywords
protein
cell cycle
cell
plants
nucleic acid
Prior art date
Application number
ARP990101634A
Other languages
Spanish (es)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR015757A1 publication Critical patent/AR015757A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Se revela un fragmento aislado de ácidos nucleicos que codifica una proteina reguladora del ciclo celular que se selecciona entre una proteina CDC2 y unaprotein quinasa de PITSLRE. Se construye un gen quimérico que codifica toda o una porcion de dicha proteína, en orientacion sentido o antisentido, en donde laexpresion de dicho gen quimérico resulta en la produccion de niveles alterados de dicha proteína regulatoria del ciclocelular en una célula huéspedtransformada. Se revelan también métodos para obtener dicho fragmento de ácidos nucleicos y un método para evaluar por lo menos un compuesto respecto de sucapacidad para inhibir la actividad de una proteína de regulacion del ciclo celular. La disponibilidad de secuencias de ácidos nucleicosque codifiquen toda ouna porcion de una proteína reguladora del ciclo celular facilita los estudios para comprender mejor la regulacion del ciclo celular en plantas, proporcionaherramientas genéticas para mejorar el crecimiento celular en cultivos detejidos, incrementa la eficacia de la transferencia de genes y ayuda a obtenertransformaciones más estables.An isolated nucleic acid fragment encoding a cell cycle regulatory protein that is selected from a CDC2 protein and a PITSLRE protein kinase is revealed. A chimeric gene is constructed that encodes all or a portion of said protein, in a sense or antisense orientation, where the expression of said chimeric gene results in the production of altered levels of said regulatory cell protein in a transformed host cell. Methods for obtaining said nucleic acid fragment and a method for evaluating at least one compound for its ability to inhibit the activity of a cell cycle regulating protein are also disclosed. The availability of nucleic acid sequences that encode any portion of a cell cycle regulatory protein facilitates studies to better understand cell cycle regulation in plants, provides genetic tools to improve cell growth in detected cultures, increases the efficiency of cell transfer. genes and helps to obtain more stable transformations.

ARP990101634A 1998-04-09 1999-04-09 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF REGULATORY PROTEINS OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER DIFFERENT METHOD AR015757A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8113298P 1998-04-09 1998-04-09

Publications (1)

Publication Number Publication Date
AR015757A1 true AR015757A1 (en) 2001-05-16

Family

ID=22162293

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP990101634A AR015757A1 (en) 1998-04-09 1999-04-09 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF REGULATORY PROTEINS OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER DIFFERENT METHOD
ARP990101633A AR015756A1 (en) 1998-04-09 1999-04-09 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A REGULATORY PROTEIN OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER THE PROOF OF A METAL

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP990101633A AR015756A1 (en) 1998-04-09 1999-04-09 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A REGULATORY PROTEIN OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER THE PROOF OF A METAL

Country Status (4)

Country Link
EP (2) EP1068334A2 (en)
AR (2) AR015757A1 (en)
AU (2) AU3478599A (en)
WO (2) WO1999053069A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3230200A (en) * 1999-02-12 2000-08-29 Pioneer Hi-Bred International, Inc. Transgenic plants with modified expression of the dp protein
BR0014634A (en) * 1999-09-24 2003-02-25 Consejo Superior Investigacion Wheat proteins and their use
CA2374431A1 (en) * 1999-09-27 2001-04-05 Pioneer Hi-Bred International, Inc. Enhanced stress tolerance in maize via manipulation of cell cycle regulatory genes
GB9923306D0 (en) 1999-10-01 1999-12-08 Isis Innovation Diagnostic and therapeutic epitope, and transgenic plant
GB0212885D0 (en) 2002-06-05 2002-07-17 Isis Innovation Therapeutic epitopes and uses thereof
US10105437B2 (en) 2004-04-28 2018-10-23 Btg International Limited Epitopes related to coeliac disease
NZ550600A (en) 2004-04-28 2010-03-26 Btg Int Ltd Epitopes related to coeliac disease
US7825293B2 (en) 2004-05-28 2010-11-02 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5294538A (en) * 1991-11-18 1994-03-15 Cold Spring Harbor Labs. Method of screening for antimitotic compounds using the CDC25 tyrosine phosphatase
US5821234A (en) * 1992-09-10 1998-10-13 The Board Of Trustees Of The Leland Stanford Junior University Inhibition of proliferation of vascular smooth muscle cell
CA2148258A1 (en) * 1992-10-29 1994-05-11 Nicholas Barrie La Thangue Transcription factor dp-1
GB9413327D0 (en) * 1994-07-01 1994-08-24 Medical Res Council Assay for inhibitors of dp-1
US5674748A (en) * 1995-03-14 1997-10-07 Thomas Jefferson University Human cyclin-dependent kinase-like proteins and methods of using the same
GB9610195D0 (en) * 1996-05-15 1996-07-24 Medical Res Council Assay
CA2257972A1 (en) * 1996-06-13 1997-12-18 Consejo Superior De Investigaciones Cientificas Plant proteins

Also Published As

Publication number Publication date
WO1999053069A3 (en) 2000-03-23
AR015756A1 (en) 2001-05-16
EP1068335A2 (en) 2001-01-17
AU3478399A (en) 1999-11-01
AU3478599A (en) 1999-11-01
EP1068334A2 (en) 2001-01-17
WO1999053069A2 (en) 1999-10-21
WO1999053075A2 (en) 1999-10-21
WO1999053075A3 (en) 2000-03-23

Similar Documents

Publication Publication Date Title
ES2609848T3 (en) New selectable marker genes
Hirai et al. Degradation of transcription factors, c‐Jun and c‐Fos, by calpain
CN107109422A (en) Use the method expressed by the Cas9 protein regulatory gene of two vector expressions
Gahan et al. Metabolic DNA as the origin of spontaneously released DNA?
ES2375058T8 (en) AXMI-010, A GENE OF DELTA-ENDOTOXIN AND METHODS FOR USE.
AR010334A1 (en) AN EXPRESSION CASSETTE AND A METHOD FOR ALTERING THE NUTRITIONAL CONTENT OF CORN SEEDS
ATE323510T1 (en) GENE THERAPEUTICAL METHODS FOR PROVIDING APOLIPOPROTEIN A-I AGONISTS AND THEIR USE FOR THE TREATMENT OF DYSLIPIDEMIC DISEASES
DK0851934T3 (en) Plants which synthesize a modified starch, methods for their preparation and modified starch
BR0312064A (en) Genes encoding pesticidal proteins
BRPI0510622A (en) polypeptide having antimicrobial activity, polynucleotide, nucleic acid construct, recombinant expression vector, recombinant host cell, method for producing a polypeptide, composition, method for killing or inhibiting microbial cell growth, use of an antimicrobial polypeptide, transgenic plant, part plant or plant cell, and animal feed additive and composition
ATE245701T1 (en) MODIFIED MUSHROOM CELLS AND METHOD FOR PRODUCING RECOMBINANT PRODUCTS
EA199900875A1 (en) PLANTS WITH CHANGED GROWTH
AR040446A1 (en) PLANTS DEFENSES
DE69834824D1 (en) NUCLEIC ACID, WHICH COATES A LEKTIN DERIVED FACTOR FOR PRESERVING PROGENITOR CELLS
BR0009471A (en) Compositions and processes for modifying gene transcription
AR016216A1 (en) ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES A POLYPEPTIDE OF AT LEAST 40 AMINO ACIDS, THAT HAS AT LEAST 80% IDENTITY, BASED ON THE CLUSTAL ALIGNMENT METHOD, WITH AN ENZYM OF THE BIOSOTHESIS OF CAROTENOIDS PLEMELAS SEQUEL, GENES TRANSFORMED GUEST, PO
BR9809516A (en) Proteins with insecticidal activities and method of use
AR026571A1 (en) PHYSCOMITRELLA PATENS MOSES GENES THAT CODIFY PROTEINS PARTICIPATING IN THE SYNTHESIS OF POLYINSATURATED AND LIPIDED FATTY ACIDS
AR015757A1 (en) ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF REGULATORY PROTEINS OF THE CELL CYCLE IN PLANTS AND SEEDS, GENQUIMERICO, CELLULA BONE TRANSFORMED, POLYPEPTIDE, METHOD TO ALTER DIFFERENT METHOD
AR016436A1 (en) PROTEIN THAT INHIBITES P27, DNA THAT CODIFIES IT, ANTIBODIES AND PRODUCTS OF DISSOLATION OF THE SAME, NUCLEIC ACID ANTISENTIDE, ARTIFICIAL STRUCTURE OF NUCLEIC ACID AND THE USE OF ALL THIS IN THERAPY AND PROPHYLAXIS OF DISEASE
ES2338285T3 (en) NEW SPECIFIC GENES OF THE BASE OF BASE TRANSFER OF ENDOSPERM CELLS (BETL).
WO2003106674A3 (en) Use of repression blocking sequences in methods for enhancing gene expression
CO4810249A1 (en) METHOD FOR THE SELECTION OF TRANSFORMED PLANTS.
ATE546539T1 (en) REVERSING THE NEGATIVE-SELECTIVE EFFECT OF NEGATIVE MARKER PROTEINS AS A SELECTION METHOD
BRPI0411227A (en) polypeptide, polynucleotide nucleic acid construction, recombinant expression vector, recombinant host cell, method for producing a polypeptide, composition, method for exterminating or inhibiting microbial cell growth, detergent composition, use of an antimicrobial polypeptide, transgenic plant, part of plant or plant cell, and animal feed additive and composition