AR013935A1 - ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A VEGETABLE ADA2 TRANSCRIPTIONAL ACTIVATOR, CHEMERIC GENE, TRANSFORMED CELLULAR, METHOD TO ALTER THE EXPRESSION LEVEL OF A HOMOLOGICAL PROTEIN OF THE ADDITIONAL METHOD OF ADDITION - Google Patents

ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A VEGETABLE ADA2 TRANSCRIPTIONAL ACTIVATOR, CHEMERIC GENE, TRANSFORMED CELLULAR, METHOD TO ALTER THE EXPRESSION LEVEL OF A HOMOLOGICAL PROTEIN OF THE ADDITIONAL METHOD OF ADDITION

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Publication number
AR013935A1
AR013935A1 ARP980103486A AR013935A1 AR 013935 A1 AR013935 A1 AR 013935A1 AR P980103486 A ARP980103486 A AR P980103486A AR 013935 A1 AR013935 A1 AR 013935A1
Authority
AR
Argentina
Prior art keywords
ada2
protein
nucleic acids
substantial portion
addition
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR013935A1 publication Critical patent/AR013935A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

El objeto principal y los accesorios están relacionados con fragmentos aislados de ácidos nucleicos que codifican toda o una porcion sustancial de unhomologo vegetal del adaptador de la transcripcion ADA2 de levadura y la construccion degenes quiméricos que codifican toda o una porcion de dichohomologo vegetal, con una orientacion en el marco de lectura o antisentido, donde la expresion del gen quimérico da como resultado la produccion de nivelesalterados de un homologo vegetalde un adaptador de transcripcion ADA2 de levadura en una célula huésped transformada. También se considera eldireccionamiento de ADA2 vegetal hacia una region promotora nueva por medio de la adicion de un dominio de union de ADN o un dominio de interaccion proteína-proteína, que conduce a un mayor nivel de expresion génica.The main object and the accessories are related to isolated fragments of nucleic acids that encode all or a substantial portion of a plant homologue of the ADA2 transcription adapter of yeast and the construction of chimeric degenes that encode all or a portion of said plant homologue, with an orientation in the reading or antisense framework, where the expression of the chimeric gene results in the production of altered levels of a plant homologue of a yeast ADA2 transcription adapter in a transformed host cell. It is also considered the direction of plant ADA2 towards a new promoter region through the addition of a DNA binding domain or a protein-protein interaction domain, which leads to a higher level of gene expression.

ARP980103486 1997-07-18 1998-07-16 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A VEGETABLE ADA2 TRANSCRIPTIONAL ACTIVATOR, CHEMERIC GENE, TRANSFORMED CELLULAR, METHOD TO ALTER THE EXPRESSION LEVEL OF A HOMOLOGICAL PROTEIN OF THE ADDITIONAL METHOD OF ADDITION AR013935A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US89717597A 1997-07-18 1997-07-18

Publications (1)

Publication Number Publication Date
AR013935A1 true AR013935A1 (en) 2001-01-31

Family

ID=25407462

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980103486 AR013935A1 (en) 1997-07-18 1998-07-16 ISOLATED FRAGMENT OF NUCLEIC ACIDS THAT CODIFIES ALL OR A SUBSTANTIAL PORTION OF A VEGETABLE ADA2 TRANSCRIPTIONAL ACTIVATOR, CHEMERIC GENE, TRANSFORMED CELLULAR, METHOD TO ALTER THE EXPRESSION LEVEL OF A HOMOLOGICAL PROTEIN OF THE ADDITIONAL METHOD OF ADDITION

Country Status (5)

Country Link
EP (1) EP1002088A1 (en)
AR (1) AR013935A1 (en)
AU (1) AU8575098A (en)
BR (1) BR9810605A (en)
WO (1) WO1999004004A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251668B1 (en) * 1998-07-13 2001-06-26 E. I. Du Pont De Nemours & Company Transcription coactivators

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2047633A1 (en) * 1990-09-12 1992-03-13 Ben Bowen Inactivation of gene transcription in plants using altered transcriptional activators
WO1993010250A1 (en) * 1991-11-18 1993-05-27 Massachusetts Institute Of Technology Transcription adaptors in eukaryotes
GB9201549D0 (en) * 1992-01-24 1992-03-11 Ici Plc Control of gene transcription
US5409823A (en) * 1992-09-24 1995-04-25 Ciba-Geigy Corporation Methods for the production of hybrid seed
GB9502456D0 (en) * 1995-02-08 1995-03-29 Univ Warwick Control of genes in transgenic plants

Also Published As

Publication number Publication date
AU8575098A (en) 1999-02-10
BR9810605A (en) 2000-07-11
EP1002088A1 (en) 2000-05-24
WO1999004004A1 (en) 1999-01-28

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