AR012983A1 - ISOLATED FRAGMENT OF NUCLEIC ACIDS CODING A VEGETABLE ACETYL TRANSHERASE HYSTER, CHEMICAL GENE, TRANSFORMED CELL HOSPED, METHOD TO ALTER THE EXPRESSION OF SUCH HISTONE, METHODS TO OBTAIN SUCH FRAGES AID - Google Patents

ISOLATED FRAGMENT OF NUCLEIC ACIDS CODING A VEGETABLE ACETYL TRANSHERASE HYSTER, CHEMICAL GENE, TRANSFORMED CELL HOSPED, METHOD TO ALTER THE EXPRESSION OF SUCH HISTONE, METHODS TO OBTAIN SUCH FRAGES AID

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Publication number
AR012983A1
AR012983A1 ARP980102823A AR012983A1 AR 012983 A1 AR012983 A1 AR 012983A1 AR P980102823 A ARP980102823 A AR P980102823A AR 012983 A1 AR012983 A1 AR 012983A1
Authority
AR
Argentina
Prior art keywords
protein
histone
expression
corn
rice
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR012983A1 publication Critical patent/AR012983A1/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se relaciona con fragmentos aislados de ácidos nucleicos que codifican toda o una porcion substancial de una proteína acetiltransferasa de maíz,arroz o trigo. La invencion también se relaciona con la construccion de un gen quimérico que codifica toda o una porcion de una proteína histonaacetiltransferasa de maíz, arroz o trigo, con orientacion en el sentido del marco de lectura o antisentido, donde la expresion del gen quimérico da comoresultado la produccion de niveles alterados de una proteína histona acetiltransferasa de maíz, arroz o trigo en una célula huésped transformada.La invencion también se relaciona con la direccion de una proteína histona acetiltransferasa de maíz, arroz o trigo hacia una nueva region promotora porla adicion de un dominio de union a ADN o de un dominio de interaccion proteína-proteína, para acetilar la histona nuclear, debilitar lainteraccion entre las histones nucleares y el ADN, abrir la estructura de la cromatina e incrementar la eficacia de la iniciacion de la transcripcion,lo cual conduce a un mayor nivel de expresion de los genes.It is associated with isolated nucleic acid fragments that encode all or a substantial portion of a corn, rice or wheat acetyltransferase protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of a corn, rice or wheat histoneacetyltransferase protein, oriented in the sense of the reading frame or antisense, where the expression of the chimeric gene results in production of altered levels of a corn, rice or wheat histone acetyltransferase protein in a transformed host cell. The invention also relates to the targeting of a corn, rice or wheat histone acetyltransferase protein to a new promoter region by the addition of a domain binding to DNA or a protein-protein interaction domain, to acetylate nuclear histone, weaken the interaction between nuclear histones and DNA, open the chromatin structure and increase the efficiency of transcription initiation, which leads at a higher level of gene expression.

ARP980102823 1997-06-12 1998-06-12 ISOLATED FRAGMENT OF NUCLEIC ACIDS CODING A VEGETABLE ACETYL TRANSHERASE HYSTER, CHEMICAL GENE, TRANSFORMED CELL HOSPED, METHOD TO ALTER THE EXPRESSION OF SUCH HISTONE, METHODS TO OBTAIN SUCH FRAGES AID AR012983A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4940897P 1997-06-12 1997-06-12

Publications (1)

Publication Number Publication Date
AR012983A1 true AR012983A1 (en) 2000-11-22

Family

ID=21959668

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980102823 AR012983A1 (en) 1997-06-12 1998-06-12 ISOLATED FRAGMENT OF NUCLEIC ACIDS CODING A VEGETABLE ACETYL TRANSHERASE HYSTER, CHEMICAL GENE, TRANSFORMED CELL HOSPED, METHOD TO ALTER THE EXPRESSION OF SUCH HISTONE, METHODS TO OBTAIN SUCH FRAGES AID

Country Status (5)

Country Link
EP (1) EP0996734A1 (en)
AR (1) AR012983A1 (en)
AU (1) AU8066398A (en)
BR (1) BR9809736A (en)
WO (1) WO1998056934A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000715A1 (en) 2001-06-22 2003-01-03 Ceres, Inc. Chimeric histone acetyltransferase polypeptides
CN110878311A (en) * 2019-10-17 2020-03-13 福建省农业科学院生物技术研究所 Rice growth and development regulation gene OsPLATZ14 and encoded protein and application thereof
CN112481231B (en) * 2020-12-09 2022-07-12 广东省微生物研究所(广东省微生物分析检测中心) Bifunctional enzyme with activities of acyltransferase and glutamic-pyruvic transaminase
CN112574981B (en) * 2020-12-30 2022-04-15 上海大学 Taraxasterol synthase, gene for coding taraxasterol synthase and preparation and application thereof
CN112920263B (en) * 2021-05-11 2021-08-10 上海浦东复旦大学张江科技研究院 Application of epigenetic modification OsMOF protein in improvement of rice yield traits
CN114634940B (en) * 2022-04-07 2024-02-20 广西大学 Corn calcineurin B protein gene and application thereof in improving drought tolerance and salt tolerance of plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650553A (en) * 1992-06-16 1997-07-22 The Trustees Of The University Of Pennsylvania Plant genes for sensitivity to ethylene and pathogens

Also Published As

Publication number Publication date
AU8066398A (en) 1998-12-30
WO1998056934A1 (en) 1998-12-17
BR9809736A (en) 2000-10-03
EP0996734A1 (en) 2000-05-03

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