AP556A

AP556A - Tetracyclic derivatives, process of preparation and use.

Info

Publication number: AP556A
Application number: APAP/P/1995/000712A
Authority: AP
Inventors: Alain Claude-Marie Daugan
Original assignee: Icos Corp
Priority date: 1994-01-21
Filing date: 1995-01-19
Publication date: 1996-11-07
Also published as: FI113176B; DE69503753D1; BG62733B1; CZ286566B6; ATE169018T1; OA10584A; AU1574895A; RU2142463C1; US6784179B2; BR9506559A; HRP950023A2; LV11690B; LU91017I2; DK0740668T3; ZA95424B; FI962927A7; CZ211696A3; US5859006A; BR9506559C8; EG25852A

Abstract

A compound of formula (1)

Description

TETRACYCLIC DERIVATIVES, PROCESS OF PREPARATION AND USE

AP. Ο Ο 5 5 6 lb

This invention relates to a series of tetracyclic derivatives, to processes for their preparation, pharmaceutical compositions containing them, and their use as therapeutic agents. In particular, the invention relates to tetracyclic derivatives which are potent and selective inhibitors of cyclic guanosine 3', 5'monophosphate specific phosphodiesterase (cGMP specific PDE) having utility in a variety of therapeutic areas where such inhibition is thought to be beneficial, including the treatment of cardiovascular disorders.

Thus, according to a first aspect, the present invention provides compounds of formula (I)

O c²⁰ and salts and solvates (e.g. hydrates) thereof, in which.

R° represents hydrogen, halogen or C-j^ alkyl;

R¹ represents hydrogen, C-|_6alkyl, C₂-e alkenyl, C₂^ alkynyl, haloC-j^alkyl, C3-8Cycloalkyl, C3-8cycloalkylC-|_3alkyl, arylCi_33lkyl or heteroarylCioalkyl;

r2 represents an optionally substituted monocyclic aromatic ring selected from benzene, thiophene, furan and pyridine or an optionally substituted bicyclic ring attached to the rest of the molecule via one of the benzene ring carbon atoms and wherein the fused ring A is a 5- or 6-membered ring which may be saturated or partially or fully unsaturated and comprises carbon atoms and optionally one or two heteroatoms selected from oxygen, sulphur and nitrogen; and

R³ represents hydrogen or C1.3 alkyl, or R¹ and R³ together represent a 3- or

4- membered alkyl or alkenyl chain.

There is further provided by the present invention a subgroup of compounds of formula (I), the subgroup comprising compounds of formula (la)_____

AP/P/ y 5 / 0 0 7 1 2

1233CV

ο ( 20 and salts and solvates (e.g. hydrates) thereof, in which:

R° represents hydrogen, halogen or C-|_6 alkyl;

R¹ represents hydrogen, C-|_galkyl, haloC-j^alkyl, C3_8cycloalkyl, C3^cycloalkylC^3alkyl, arylC-|_3alkyl or heteroarylC-i_3^alkyl; and

R2 represents an optionally substituted monocyclic aromatic ring selected from benzene, thiophene, furan and pyridine or an optionally substituted bicyclic o© ring attached to the rest of the molecule via one of the benzene ring carbon atoms and wherein the fused ring A is a 5- or 6-membered ring which may be saturated or partially or fully unsaturated and comprises carbon atoms and optionally one or two heteroatoms selected from oxygen, sulphur and nitrogen.

Within R¹ above, the term aryl as part of an arylC-|_3alkyl group means phenyl or phenyl substituted by one or more (e.g. 1, 2 or 3) substituents selected from halogen, C-i-galkyl, C-|^alkoxy and methylenedioxy. The term heteroaryl as part of a heteroarylC-|_3alkyl group means thienyl, furyl or pyridyl each optionally substituted by one or more (e.g. 1, 2 or 3) substituents selected from halogen, C<|_6 alkyl and C-j^alkoxy. The term C3_gcycloalkyl as a group or part of a C3-8cycloalkylC-|_3^a,kyl group means a monocyclic ring comprising three to eight carbon atoms. Examples of suitable cycloalkyl rings include the C₃^cycloalkyl rings cyclopropyl, cyclobutyl, cyciopentyl and cyclohexyl.

Within r2 above, optional benzene ring substituents are selected from one or more (e.g. 1, 2 or 3) atoms or groups comprising halogen, hydroxy, C-|^alkyl, Cj ^alkoxy, -CO₂R^b, haloC^alkyl, haloC-j ^alkoxy, cyano, nitro and NR^aR^b, where R^a and R^b are each hydrogen or C-|_galkyl, or R^a may also represent C2-7alkanoyl or C-|^alkylsulphonyl. Optional substituents for the remaining ring systems are selected from one or more (e.g. 1, 2 or 3) atoms or groups comprising halogen, C^alkyl, Cj^alkoxy and arylCi-₃alkyl as defined above.

AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6

The bicyclic ring may, for example, represent naphthalene, a heterocycle such as benzoxazole, benzothiazole, benzisoxazole, benzimidazole, quinoline, indole, benzothiophene or benzofuran or ^Y (where n is an integer 1 or 2 and X and Y may each represent

CH₂, 0, S or NH).

In the above definitions, the term alkyl as a group or part of a group means a straight chain or, where available, a branched chain alkyl moiety. For example, it may represent a Ci^alkyl function as represented by methyl, ethyl, ( , n-propyl, i-propyl, n-butyl, s-butyl and t-butyl. The term ‘alkenyl’ as used herein includes straight-chained and branched alkenyl groups, such as vinyl and allyl groups. The term ’alkynyl’ as used herein includes straight-chained and branched alkynyl groups, suitably acetylene. The term halogen herein means a fluorine, chlorine, bromine or iodine atom. The term haloC i^alkyl means an alkyl group as defined above comprising one to six carbon atoms substituted at 15 one or more carbon atoms by one or more (e.g. 1, 2 or 3) halogen atoms.

Similarly, a haloC-|_6alkoxy group is a haloCnjalkyl group as defined above linked to the R² benzene ring via an oxygen atom. Examples of haloC i^alkyl groups include trifluoromethyl and 2,2,2-trifluoroethyl. An example of a haloC ή ^alkoxy group is trifluoromethoxy. The term C2-7alkanoyl means a C’20 Cj^alkylcarbonyl group where the Chalky I portion is as defined above. An example of a suitable C2-7alkanoyl group is the C2alkanoyl group acetyl.

( It will be appreciated that when R° is a halogen atom or a C^alkyl group this substituent may be sited at any available position on the phenyl portion of the tetracyclic ring. However, a particular site of attachment is the ring 1025 position.

The compounds of formula (I) may contain two or more asymmetric centres and thus can exist as enantiomers or diastereoisomers. In particular, in formula (I) above two ring chiral centres are denoted with asterisks. It is to be understood that the invention includes both mixtures and separate individual isomers of the compounds of formula (I).

The compounds of formula (I) may also exist in tautomeric forms and the invention includes both mixtures and separate individual tautomers thereof.__

AP/P/ 9 5 / 0 0 7 1 2

1233CV

The pharmaceutically acceptable salts of the compounds of formula (I) which contain a basic centre are acid addition salts formed with pharmaceutically acceptable acids. Examples include the hydrochloride, hydrobromide, sulphate or bisulphate, phosphate or hydrogen phosphate, acetate, benzoate, succinate, fumarate, maleate, lactate, citrate, tartrate, gluconate, methanesulphonate, benzenesulphonate and p-toluenesulphonate salts. Compounds of the formula (I) can also provide pharmaceutically acceptable metal salts, in particular alkali metal salts, with bases. Examples include the sodium and potassium salts.

A particular group of compounds of the invention are those compounds of 10 formula (I) in which R° is hydrogen or halogen (e.g. fluorine), especially hydrogen.

Another particular group of compounds of the invention are those compounds of formula (I) in which R¹ represents hydrogen, Cj^alkyl, haloC-i^alkyl,, C3^cycloalkyl, C3^cycloalkylmethyl, pyridylC-|_3alkyl, furylC-j_3alkyl or optionally substituted benzyl. Within this particular group of compounds, examples of C-|^alkyl groups are methyl, ethyl, n-propyl, i-propyl and n-butyl. Examples of C3_6cycloalkylmethyl groups are cyclopropylmethyl and cyclohexylmethyl. Examples of optionally substituted, benzyl groups include benzyl and halobenzyl (e g. fluorobenzyl).

A further particular group of compounds of the invention are those compounds of formula (I) in which R² represents an optionally substituted benzene, thiophene, furan, pyridine or naphthalene ring or an optionally

Οζ>>· substituted bicyclic ring ^Y (where n is 1 or 2 and X and Y are each CH2 or O). Within this particular group of compounds, examples of substituted benzene groups are benzene substituted by one of halogen (e.g. chlorine), hydroxy, C-j_3alkyl (e.g. methyl, ethyl or i-propyl), Ci^alkoxy (eg. methoxy or ethoxy), -CO₂R^b, halomethyl (e.g. trifluoromethyl), halomethoxy (e g. trifluoromethoxy), cyano, nitro or NR^aR^b where R^a and R^b are each hydrogen or methyl or R^a is acetyl; or benzene substituted by dihalo (e.g. dichloro) or by

C-|.3alkoxy (eg methoxy) and one of halogen (e g. chlorine) and hydroxy. An example of a substituted thiophene ring is a halo (e.g. bromo) substituent thiophene ring.-AP/P/ 9 5 / 0 0 7 1 2

AP. 0 0 5 5 6

A still further particular group of compounds of formula I are those wherein R³represents hydrogen or R¹ and R³ together represent a 3-membered alkyl chain.

A preferred group of compounds of the invention are the cis isomers of

( · and mixtures thereof with their cis optical enantiomers, including racemic mixtures, and salts and solvates (e.g. hydrates) of these compounds in which R° is hydrogen or halogen (e.g. fluorine), especially hydrogen and R¹, R and R³are as defined previously.

The single isomers represented by formula (Ib), i.e. the 6R, 12aR isomers, are particularly preferred.

Within the above definitions R^ may preferably represent C^alkyl (e.g. methyl, ethyl, i-propyl and n-butyl), C3^cycloalkyl (eg. cyclopentyl) or C3-6Cycloalkylmethyl (e.g. cyclopropylmethyl).

R² may preferably represent a substituted benzene ring such as benzene substituted by C-|_3alkoxy (e.g. methoxy) or by Ci_3alkoxy (e.g. methoxy) and halogen (e.g. chlorine), particularly 4-methoxyphenyl or 3-chloro-4methoxyphenyl, or R² may preferably represent 3,4-methylenedioxyphenyl.

It is to be understood that the present invention covers all appropriate combinations of particular and preferred groupings hereinabove.

Particular individual compounds of the invention include: Cis-2,3,6,7,12,12a-hexahydro-2-(4-pyridylmethyl)-6-(3,4methylenedioxyphenyl)-pyrazino[2', Γ : 6,1 ]pyrido[3,4-b]indole-1,4-dione;

Cis-2,3,6,7,12,12a-hexahydro-6-(2,3-dihydrobenzo[b)furan-5-yl)-2-methylpyrazino[2', T:6,1 ]py rido[3,4-b]i ndo le -1,4-dione;

Cis-2,3,6,7,12,12a-hexahydro-6-(5-bromo-2-thienyl)-2-methylpyrazino^'.TO.IlpyridotS^-blindole -1,4-dione;

Cis-2,3,6,7,12,12a-hexahydro-2-butyl-6-(4-methylphenyl)pyrazino[2’, 1':6,1 ]pyrido[3,4-b]indole -1,4-dione; (6R,12aR)-2,3,6,7,12,12a-Hexahydro-2-isopropyl-6-(3,4methylenedioxyphenyl)-pyrazino[2’,T:6,1]pyrido[3,4-b]indole -1,4-dione;-AP/P/ 9 5 / 0 0 7 12

1233CV (6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-cyclopentyl-6-(3,4methylenedioxyphenyl)-pyrazino[2',r:6,1]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-cyclopropylmethyl-6-(4-methoxyphenyl)pyrazino[2', Γ:6,1 ]pyrido[3,4-b]indole -1,4-dione;

(eR.^aR^.S.ej.^.^a-Hexahydro-e-iS-chloro^-methoxyphenyO^-methylpyrazino[2', 1 ':6,1]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-methyl-6-(3,4-methylenedioxyphenyl)pyrazino[2', 1 ’ :6,1 ]pyrido[3,4-b]indole-1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-6-(3,4-methylenedioxyphenyl)10 pyrazino[2', 1': 6,1] pyrido [3,4-b] indole-1,4-dione;

' (5aR, 12R, 14aS)-1,2,3,5,6,11,12,14a-Octahydro-12-(3,4methylenedioxyphenyl)-pyrrolo[1,2: 4',5’]pyrazino[2'₁T : 6,1 ]pyrido[3,4b]indole-5-1,4-dione;

and physiologically acceptable salts and solvates (e.g. hydrates) thereof.

A specific compound of the invention is:

(6R, 12aR)-2,3,6,7,12,12a-hexahydro-2-methyl-6-(3,4-methylenedioxyphenyl)pyrazino[2’, 1 ':6,1 ]pyrido[3,4-b]indole -1,4-dione;

and physiologically acceptable salts and solvates (e.g. hydrates) thereof.

It has been shown that compounds of the present invention are potent and selective inhibitors of cGMP specific PDE. Thus, compounds of formula (I) are of interest for use in therapy, specifically for the treatment of a variety of conditions where inhibition of cGMP specific PDE is thought to be beneficial.

q As a consequence of the selective PDE V inhibition exhibited by compounds of the present invention, cGMP levels are elevated, which in turn can give rise p 25 to beneficial anti-platelet, anti-neutrophil, anti-vasospastic, vasodilatory, natriuretic and diuretic activities as well as potentiation of the effects of endothelium-derived relaxing factor (EDRF), nitrovasodilators, atrial natriuretic factor (ANF), brain natriuretic peptide (BNP), C-type natriuretic peptide (CNP) and endothelium-dependent relaxing agents such as bradykinin, acetylcholine and 5-HT·,. The compounds of formula (I) therefore have utility in the treatment of a number of disorders, including stable, unstable and variant (Prinzmetal) angina, hypertension, pulmonary hypertension, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency (e.g. postpercutaneous transluminal coronary angioplasty), peripheral vascular disease, vascular disorders such as Raynaud's disease, inflammatory diseases, stroke,

AP/P/ 9 5 / 0 0 7 1 2

AP . Ο Ο 5 5 6 bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma and diseases characterised by disorders of gut motility (e.g. irritable bowel syndrome).

It will be appreciated that references herein to treatment extend to 5 prophylaxis as well as treatment of established conditions.

It will also be appreciated that 'a compound of formula (I),' or a physiologically acceptable salt or solvate thereof can be administered as the raw compound, or as a pharmaceutical composition containing either entity.

There is thus provided as a further aspect of the invention a compound of 10 formula (I) for use in the treatment of stable, unstable and variant (Prinzmetal) angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of ⁽ reduced blood vessel patency, (e.g. post-PTCA), peripheral vascular disease, vascular disorders such as Raynaud's disease, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut motility (e.g. IBS).

According to another aspect of the invention, there is provided the use of a compound of formula (I) for the manufacture of a medicament for the treatment of stable, unstable and variant (Prinzmetal) angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure,— renal failure, atherosclerosis, conditions of reduced blood vessel patency, (e g. post-PTCA), peripheral vascular disease, vascular disorders such as Raynaud’s θ disease, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut ζ- 25 motility (e g. IBS).

In a further aspect, the invention provides a method of treating stable, unstable and variant (Prinzmetal) angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, (e.g.

post-PTCA), peripheral vascular disease, vascular disorders such as Raynaud’s disease, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut motility (e.g. IBS) in a human or non-human animal body which comprises administering to said body a therapeutically effective amount of a compound with formula (I).--—--AP/P/ 9 5 / 0 0 7 1 2

1233CV

Compounds of the invention may be administered by any suitable route, for example by oral, buccal, sub-lingual, rectal, vaginal, nasal, topical or parenteral (including intravenous, intramuscular, subcutaneous and intracoronary) administration. Oral administration is generally preferred.

For administration to man in the curative or prophylactic treatment of the disorders identified above, oral dosages of a compound of formula (I) will generally be in the range of from 0.5-800mg daily for an average adult patient (70kg). Thus for a typical adult patient, individual tablets or capsules contain from 0.2-400mg of active compound, in a suitable pharmaceutically acceptable vehicle or carrier, for administration in single or multiple doses, once or several times per day. Dosages for intravenous, buccal or sublingual administration will typically be within the range of from 0.1-400 mg per single dose as required. In ( ' practice the physician will determine the actual dosing regimen which will be most suitable for an individual patient and it will vary with the age, weight and response of the particular patient. The above dosages are exemplary of the average case but there can be individual instances in which higher or lower dosage ranges may be merited, and such are within the scope of this invention.

For human use, a compound of the formula (I) can be administered alone, but will generally be administered in admixture with a pharmaceutical carrier selected with regard to the intended route of administration and standard pharmaceutical practice. For example, the compound may be administered orally, buccally or sublingually, in the form of tablets containing excipients such

Cas starch or lactose, or in capsules or ovules either alone or in admixture with (

excipients, or in the form of elixirs or suspensions containing flavouring or colouring agents. Such liquid preparations may be prepared with pharmaceutically acceptable additives such as suspending agents (e g. methylcellulose, a semi-synthetic glyceride such as witepsol or mixtures of glycerides such as a mixture of apricot kernel oil and PEG-6 esters or mixtures of PEG-8 and caprylic/capric glycerides). A compound may also be injected 30 parenterally, for example intravenously, intramuscularly, subcutaneously or intracoronarily. For parenteral administration, the compound is best used in the form of a sterile aqueous solution which may contain other substances, for example salts, or monosaccharides such as mannitol or glucose, to make the solution isotonic with blood.-----AP/P/ 95/00712 ί

AP.00556

Thus, the invention provides in a further aspect a pharmaceutical composition comprising a compound of the formula (I) together with a pharmaceutically acceptable diluent or carrier therefor.

There is further provided by the present invention a process of preparing a pharmaceutical composition comprising a compound of formula (I), which process comprises mixing a compound of formula (I) together with a pharmaceutically acceptable diluent or carrier therefor.

A compound of formula (I) may also be used in combination with other therapeutic agents which may be useful in the treatment of the above-mentioned disease states. The invention thus provides, in another aspect, a combination of a compound of formula (I) together with another therapeutically active agent.

The combination referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical compositions comprising a combination as defined above together with a pharmaceutically acceptable diluent or carrier comprise a further aspect of the invention.

The individual components of such a combination may also be administered either sequentially or simultaneously in separate pharmaceutical formulations.

Appropriate doses of known therapeutic agents for use in combination with a compound of formula (I) will be readily appreciated by those skilled in the art.

Compounds of formula (I) may be prepared by any suitable method known in the art or by the following processes which form part of the present invention. In the methods below R°, and R^ are as defined in formula (I) above unless otherwise indicated.

Thus, a process (A) for preparing a compound of formula (I) wherein R³represents hydrogen comprises treating a compound of formula (II)

OAlk

CHjHal (Π) (in which Aik represents C^alkyl, e.g. methyl or ethyl and Hal is a halogen atom, e.g. chlorine) with a primary amine R¹NH2 in a suitable solvent such as an alcohol (e.g. methanol or ethanol) or a mixture of solvents, conveniently at a temperature of from 20°C to reflux (e g. at about 50°C).

A compound of formula (II) may conveniently be prepared by treating a compound of formula (III)-AP/P/ 9 5 / 0 0 7 1 2

1233CV

Ο 20 (

with a haloacetyl halide (e g. chloroacetyl chloride) in a suitable solvent such as a halogenated hydrocarbon (e g. trichloromethane or dichloromethane), or an ether (e.g. tetrahydrofuran), preferably in the presence of a base such as an organic amine (e.g. a trialkylamine such as triethylamine) or an alkali metal carbonate or bicarbonate (e.g. NaHCO3). The reaction may conveniently be effected at a temperature of from -20°C to +20°C (e.g. at about O°C).

A compound of formula (I) may also be prepared from a compound of formula (III) in a two-step procedure via a compound of formula (II) isolated without purification.

Compounds of formula (I) may be prepared as individual enantiomers in two steps from the appropriate enantiomer of formula (III) or as mixtures (e.g. racemates) of either pairs of cis or trans isomers from the correspondong mixtures of either pairs of cis or trans isomers of formula (III).

Individual enantiomers of the compounds of the invention may be prepared from racemates by resolution using methods known in the art for the separation of racemic mixtures into their constituent enantiomers, for example using HPLC (high performance liquid chromatography) on a chiral column such as Hypersil naphthylurea.

A compound of formula (III) may conveniently be prepared from a tryptophan alkyl ester of formula (IV)

AP/P/ 9 5 / 0 0 7 1 2

OAlk (IV) (where Aik is as previously defined) or a salt thereof (e.g. the hydrochloride salt) according to either of the following procedures (a) and (b). Procedure (b) is only suitable for preparing cis isomers of formula (III) and may be particularly suitable for preparing individual cis enantiomers of formula (III) from D- or Ltryptophan alkyl esters as appropriate. —______________________

AP .00556

Procedure (a)

This comprises a Pictet-Spengler cyclisation between a compound of formula (IV) and an aldehyde R2CHO. The reaction may conveniently be effected in a suitable solvent such as a halogenated hydrocarbon (e g. dichloromethane) or an aromatic hydrocarbon (e.g. toluene) in the presence of an acid such as trifluoroacetic acid. The reaction may conveniently be carried out at a temperature of from -20°C to reflux to provide a compound of formula (III) in one step. The reaction may also be carried out in a solvent such as an aromatic hydrocarbon (e.g. benzene or toluene) under reflux, optionally using a Dean10 Stark apparatus to trap the water produced.

The reaction provides a mixture of cis and trans isomers which may be either individual enantiomers or racemates of pairs of cis or trans isomers depending upon whether racemic or enantiomerically pure tryptophan alkyl ester was used as the starting material. Individual cis or trans enantiomers may conveniently be separated from mixtures thereof by fractional crystallisation or by chromatography (e.g. flash column chromatography) using appropriate solvents and eluents. Similarly, pairs of cis and trans isomers may be separated by chromatography (e.g. flash column chromatography) using appropriate eluents. An optically pure trans isomer may also be converted to an optically pure cis isomer using suitable epimerisation procedures. One such procedure comprises treating the trans isomer or a mixture (e.g. 1 : 1 mixture) of cis and trans isomers with methanolic or aqueous hydrogen chloride at a temperature of from 0°C to the refluxing temperature of the solution. The mixture may then be subjected to chromatography (e.g. flash column chromatography) to separate > 25 the resulting diastereoisomers, or in the procedure utilising aqueous hydrogen chloride the desired cis isomer precipitates out as the hydrochloride salt which may then be isolated by filtration.

Procedure (b)

This comprises a four-step procedure from a compound of formula (IV) or a salt thereof (e.g. the hydrochloride salt). The procedure is particularly suitable for preparing a 1R, 3R isomer of formula (III) from a D-tryptophan alkyl ester of formula (IV) or a salt thereof (e g. the hydrochloride salt). Thus, a first step (i) comprises treating a compound of formula (IV) with an acid halide R2C0Hal (where Hal is as previously defined) in the presence of a base, e.g. an organic

AP/P/ 9 5 / 0 0 7 1 2

1233CV base such as a trialkylamine (for example triethylamine), to provide a compound

(V)

The reaction may be conveniently carried out in a suitable solvent such as a halogenated hydrocarbon (e.g. dichloromethane) or an ether (e.g. tetrahydrofuran) and at a temperature of from -20°C to +40°C.

Step (ii) comprises treating a compound of formula (V) with an agent to convert the amide group to a thioamide group. Suitable sulfurating agents are well-known in the art. Thus, for example, the reaction may conveniently be effected by treating (V) with Lawesson's reagent. This reaction may conveniently be carried out in a suitable solvent such as an ether (e.g. dimethoxyethane) or an aromatic hydrocarbon (e.g. toluene) at an elevated temperature such as from 40°C to 80°C to provide a compound of formula (VI)

(VI)

Step (iii) comprises treating a compound of formula (VI) with a suitable agent to provide a compound of formula (VII) ·

(VII) (where Hal is a halogen atom, e.g. iodine). The reaction may conveniently be effected by treating (VI) with an alkylating agent such as a methyl halide (e.g. methyl iodide) or an acylating agent such as an acetyl halide (e.g. acetyl chloride) in a suitable solvent such as a halogenated hydrocarbon (e.g. dichloromethane) at an elevated temperature (e.g. under reflux).

In step (iv) the resulting iminium halide of formula (VII) may be treated with a reducing agent such as boron hydride, e.g. sodium borohydride, to provide the desired compound of formula (III). The reduction may conveniently be effected

AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6 at a low temperature, e.g. within the range of -1OO°C to O°C, in a suitable solvent such as an alcohol (e.g. methanol).

There is further provided by the present invention a process (B) for preparing a compound of formula (I), wherein R¹ and R³ together represent a 3- or 4membered alkyl or alkenyl chain, which process (B) comprises cyclisation of a compound of formula (VIII)

(VIII) ( wherein Alk represents C^alkyl and R¹ and R³ together represent a 3- or 410 membered chain both as hereinbefore described. The cyclisation is suitably carried out in an organic solvent or solvents, such as an alcoholic solvent (e.g. methanol) and optionally an ether solvent such as tetrahydrofuran, and in the presence of a reducing agent, aptly a palladium catalyst, such as palladium on carbon.

Conveniently a compound of formula (VIII) is prepared by reaction of a compound of formula (III) as hereinbefore described with a compound of formula (IX)

AP/r/ 3 5 / 0 0 7 1 2 wherein Hal represents a halogen atom as hereinbefore described, R¹ and R³together represent a 3- or 4-membered chain as hereinbefore described and R⁴represents a protecting group, suitably a benzyloxycarbonyl group or the like. Typically the reaction is carried out in a chlorinated organic solvent, such as dichloromethane, and a tertiary amine, such as triethylamine or the like.

According to a further aspect of the present invention, there is provided a process (C) for preparing a compound of formula (I) wherein R³ represents Ci. ₃alkyl, which process comprises cyclisation of a compound of formula (X)______

1233CV

(X) wherein Aik represents C^alkyl as hereinbefore described and R⁵ represents 5 C₂-5alkyl, substituted at Ci by a halogen atom, the halogen atom being as hereinbefore described. Suitably the cyclisation is achieved by reflux for many

C hours, such as 22 to 26 hours, in the presence of an ether solvent, such as tetrahydrofuran, and a suitable amine as hereinafter described in the ( accompanying examples.

Aptly a compound of formula (X) can be prepared from a compound of formula (III) by suitable acylation techniques, such as reaction with a C>scarboxylic acid, substituted at C₂ by a halogen atom in a halogenated organic solvent, such as dichloromethane.

Compounds of formula (I) may be converted to other compounds of formula (I). Thus, for example, when R^ is a substituted benzene ring it may be necessary or desirable to prepare the suitably substituted compound of formula (I) subsequent to process (A), (B) or (C) as above. Examples of appropriate interconversions include nitro to amino or aralkyloxy to hydroxy by suitable reducing means (e.g. using a reducing agent such as SnCl2 or a palladium

O20 catalyst, such as palladium-on-carbon), or amino to substituted amino such as acylamino or sulphonylamino using standard acylating or sulphonylating conditions. In the case where R² represents a substituted bicyclic system, suitable interconversion can involve removal of a substituent, such as by treatment with a palladium catalyst (e.g. palladium-on-carbon) whereby, for example, a benzyl substituent may be removed from a suitable bicyclic system.

The pharmaceutically acceptable acid addition salts of the compounds of formula (I) which contain a basic centre may be prepared in a conventional manner. For example, a solution of the free base may be treated with a suitable acid, either neat or in a suitable solution, and the resulting salt isolated either by filtration or by evaporation under vacuum of the reaction solvent.

Pharmaceutically acceptable base addition salts may be obtained in an

AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6 analogous manner by treating a solution of a compound of formula (I) with a suitable base. Both types of salt may be formed or interconverted using ionexchange resin techniques.

Compounds of the invention may be isolated in association with solvent molecules by crystallisation from or evaporation of an appropriate solvent.

Thus, according to a further aspect of the invention, we provide a process for preparing a compound of formula (I) or a salt or solvate (e g. hydrate) thereof which comprises process (A), (B) or (C) as hereinbefore described followed by

i) an interconversion step; and/or either ii) salt formation; or iii) solvate (e.g. hydrate) formation.

There is further provided by the present invention compounds of formulae (II), (VIII), (X) and further compounds of formulae (III), (V), (VI) and (VII), with the exception for compounds (III), (V), (VI) and (VII) wherein R° is hydrogen, R² is phenyl and Aik is methyl.

The synthesis of the compounds of the invention and of the intermediates for use therein are illustrated by the following, non-limiting Examples. In the Examples section hereinafter the following abbreviations are used:

DMSO (dimethylsulphoxide), MeOH (methanol), EtOH (ethanol), DMF (dimethylformamide), EtOAc (ethyl acetate) and THF (tetrahydrofuran).

Intermediates 1 and 2

Methyl 1.2,3,4-tetrahydro-1 -(3,4-methylenedioxyphenyl)-9H-pyridof3,425 blindole-3-carboxvlate, cis and trans isomers

To a stirred solution of racemic tryptophan methyl ester (13 g) and piperonal (9.7 g) in anhydrous CH2CI2 (300 mL) cooled at 0°C was added dropwise trifluoroacetic acid (9 mL) and the solution was allowed to react at ambient temperature. After 4 days, the yellow solution was diluted with CH2CI2 (100 mL), washed with a saturated aqueous solution of NaHCO3, then with water and dried over Na2SC>4. The organic layer was evaporated to dryness under reduced pressure and the residue was purified by flash chromatography eluting with CH2Cl2/MeOH (99/1) to give first Intermediate 1. the cis isomer (6.5 g) m.p.

: 90-93°C followed by Intermediate 2. the trans isomer (6.4 g) m.p. : 170°C. ..... ----AP/P,' 9 5 / 0 0 7 1 2

1233CV

The following compounds were obtained in a similar manner:

Intermediates 3 and 4

Methyl 1.2.3,4-tetrahydro-1 -(4-methoxvphenvl)-9H-pyrido[3.4-blindole-35 carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 4methoxybenzaldehyde gave Intermediate 3. the cis isomer as white crystals m.p.; 142°C and Intermediate 4. the trans isomer as white crystals m.p.: 209210°C.

Intermediate 5

Methyl 1,2,3,4-tetrahvdro-1 -(3-methoxyphenvl)-9H-pvridof3,4-b1indole-3carboxylate, cis isomer

The same method but starting from racemic tryptophan methyl ester and 315 methoxybenzaldehyde gave the title compound as white crystals m.p. : 146°C.

Intermediates 6 and 7

Methyl 1,2.3,4-tetrahydro-1 -(4-ethoxvphenyl)-9H-pyridof3,4-blindole-320 carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 4ethoxybenzaldehyde gave Intermediate 6, the cis isomer as white crystals m.p.;

_r 180^eC and Intermediate 7. the trans isomer as white crystals m.p. : 196-198’C.

' 25 Intermediates 8 and 9

Methyl 1.2,3.4-tetrahvdro-1 -(2,3-dihvdrobenzofb1furan-5-vl)-9H-pyrido[3.4blindole-3-carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 2,3dihydrobenzo[b]furan-5- carboxaldehyde gave Intermediate 8. the cis isomer as white crystals m.p. ; 106-109°C and Intermediate 9. the trans isomer as white crystals m.p. : 219-222’C.

Intermediates 10 and 11

Methyl 1.2,3,4-tetrahydro-1 -(3.4-ethvlenedioxvphenvl)-9H-pyridof3.4-b1indole-335 carboxylate, cis and trans isomers

AP/P,' 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6

The same method but starting from racemic tryptophan methyl ester and 1,4benzodioxan-6-carboxaldehyde gave Intermediate 10, the cis isomer as white crystals m.p. ; 104-106°C and Intermediate 11. the trans isomer as white crystals m.p. : 207-209°C.

Intermediate 12

Methyl 1,2,3,4-tetrahydro-1 -(2-chlorophenvl)-9H-pyridof3,4-b1indole-3carboxylate, mixture of cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 210 chlorobenzaldehyde gave the title compound as white crystals m.p.: 154°C.

/- Intermediates 13 and 14 < ) Methyl 1,2,3.4-tetrahydro-1-(4-chlorophenvl)-9H-pvridof3.4-b1indole-3carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 4chlorobenzaldehyde gave Intermediate 13. the cis isomer as white crystals m.p.

: 208-209°C and Intermediate 14. the trans isomer as white crystals m.p. : 108109°C.

Intermediates 15 and 16

Methyl 1,2,3,4-tetrahvdro-1 -(3,4-dichlorophenyl)-9H-pyridof3.4-b1indole-3carboxvlate, cis and trans isomers

Qj The same method but starting from racemic tryptophan methyl ester and 3,4dichlorobenzaldehyde gave Intermediate 15. the cis isomer as a white solid ¹H

C 25 NMR (CDCI3) δ (ppm): 7.8-7 (m, 8H, H aromatic); 5.15 (brs, 1H, H-1); 3.9 - 3.8 (dd, 1H, H-3) 3.7 (s, 3H, CO₂CH₃) ; 3.2 - 3.1 (ddd, 1H, H-4) 2.9 (m, 1H, H-4) ;

2.4 (brs, 1H, NH) and Intermediate 16, the trans isomer as a white solid m.p. ; 204°C.

Intermediate 17

Methyl 1,2,3,4-tetrahvdro-1-(1.2,3,4-tetrahvdro-6-naphthvl)-9H-pyridof3.4blindole-3-carboxylate, cis isomer

The same method but starting from racemic tryptophan methyl ester and 1,2,3,4tetrahydronaphthyl-6- carboxaldehyde gave the title compound as a white solid ¹H NMR (CDCI3) δ (ppm): 7.7-7(m, 8H, H aromatic); 5.2 (s, 1H, H-1); 4.0 (dd,

AP/P/ 9 5 / 0 0 7 1 2

1233CV

Η, Η-3); 3.8 (s, 3Η, CO2CH3); 3.2 (m, 1 Η, Η-4); 3.0 (m, 1 Η, Η-4); 2.7 (m, 4Η, CH2A1·); 1.7 (s, 4Η, CH₂CH₂Ar)

Intermediates 18 and 19

Methvl 1,2,3.4-tetrahvdro-1 -(2-naphthvl)-9H-pvridof3.4-b1indole-3-carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 2naphthaldehyde gave Intermediate 18. the cis isomer as a white solid ¹H NMR (CDCI3) δ (ppm): 8-6.9 (m, 12H, H aromatic); 5.4 (s, 1H, H-1); 3.95 (dd, 1H, H10 3) ; 3.7 (s, 3H, CO2CH3) 3.2 (ddd, 1H, H-4) ; 3 (m, 1H, H-4) ; 2.5 (brs, 1H, NH) and Intermediate 19, the trans isomer as a white solid (0.6 g) m.p. : 119°C.

Intermediates 20 and 21

Methvl 1,2,3.4-tetrahvdro-1 -(2-thienyl)-9H-pvridof3.4-blindole-3-carboxvlate. cis 15 and trans isomers

The same method but starting from racemic tryptophan methyl ester and 2thiophenecarboxaldehyde gave Intermediate 20, the cis isomer as a pale yellow solid m.p. : 134-137°C and Intermediate 21. the trans isomer as white crystals m.p. :169°C.

Intermediates 22 and 23

Ethvl 1.2.3.4-tetrahvdro-1-(3-thienvl)-9H-pvridof3,4-b1indole-3-carboxvlate. cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 325 thiophenecarboxaldehyde gave Intermediate 22. the cis isomer as white crystals m.p. : 130°C and Intermediate 23. the trans isomer as white crystals m.p. :182184°C.

Intermediates 24 and 25

Methvl 1,2,3.4-tetrahvdro-1 -(5-bromo-2-thienyl)-9H-pyridof3,4-b1indole-3carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 5bromo-2-thiophenecarboxaldehyde gave Intermediate 24, the cis isomer as a cream solid m.p.. 130°C and Intermediate 25, the trans isomer as a cream solid

m.p. : 205°C. --------------------------------—--------AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6

Intermediates 26 and 27

Methvl f,2,3,4-tetrahvdro-1-(4-bromo-2-thienvl))-9H-pyridof3.4-b1indole-3carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 4bromo-2-thiophenecarboxaldehyde gave Intermediate 26, the cis isomer as a cream solid m.p.: 200°C and Intermediate 27. the trans isomer as a cream solid m.p.: 120°C.

Intermediate 28

Methvl 1,2.3.4-tetrahydro-1 -(3-furvl)-9H-pyridof3.4-b1indole-3-carboxvlate, mixture of cis and trans isomers

C ' The same method but starting from racemic tryptophan methyl ester and 3furaldehyde gave the title compound as a yellow solid m.p. : 130°C.

Intermediates 29 and 30

Ethvl 1.2,3,4-tetrahydro-1 -(5-methvl-2-furyl)-9H-pvridof3.4-b1indole-3carboxvtate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 520 methylfurfural gave Intermediate 29, the cis isomer as a oily compound ¹H NMR (CDCI3) δ (ppm): 7.7 (brs, 1H, NH indole); 7.5 (d, 1 Η, H aromatic); 7.25-6.9 (m, 3H, H aromatic); 6.15 (d, 1H, H aromatic); 5.85 (m, 1H, H aromatic); 5.25 (brs, q 1H, H-1); 4.2 (q, 2H, CO₂CH₂CH₃); 3.8 (dd, 1H, H-3); 3.2-2.8 (m, 2H, H^4); 2.2 (s, 3H, CH3); 1.25 (t, 3H, 0Ο₂0Η₂0Η₃) and Intermediate 30. the trans isomer ( 25 as a cream solid m.p. : 152°C.

Intermediates 31 and 32

Ethvl 1.2.3.4-tetrahvdro-1 -(4-methylphenyD-9H-pyridof3,4-b1indole-3carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and ptolualdehyde gave Intermediate 31. the cis isomer as white crystals m.p. : 148°C and Intermediate 32, the trans isomer as white crystals m.p. : 180°C.

Intermediates 33 and 34

1233CV

Methyl 1,2,3,4-tetrahvdro-1 -(3-methvlphenyl)-9H-pyrido[3,4-blindole-3carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and mtolualdehyde gave Intermediate 33. the cis isomer as white crystals ¹H NMR (CDCI3) 6(ppm): 7.6-7 (m, 9H, H aromatic); 5.2 (brs, 1H. H-1); 4-3.9 (dd, 1 Η, H3) 3.8 (s, 3H, CO₂CH₃) ; 3.2 - 3.1 (ddd, 1H, H-4) 3 (m, 1H, H-4) ; 2.35 (s, 3H, CH3); 1.7 (brs, 1H, NH) and Intermediate 34. the trans isomer as a white solid m.p. : 175°C.

Intermediates 35 and 36

Methyl 1,2,3,4-tetrahvdro-1 -(4-trifluoromethvlphenvl)-9H-pyridof3.4-blindole-3carboxvlate, cis and trans isomers · The same method but starting from racemic tryptophan methyl ester and 4trifluoromethylbenzaldehyde gave Intermediate 35. the cis isomer as pale yellow crystals m.p. : 190’C and Intermediate 36. the trans isomer as pale yellow crystals m.p.: 203°C.

Intermediates 37 and 38

Ethvl 1,2,3,4-tetrahvdro-1 -(4-cyanophenvl)-9H-pyridof3.4-blindole-320 carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 4cyanobenzaldehyde gave Intermediate 37. the cis isomer as white crystals m.p.

θ : 200°C and Intermediate 38. the trans isomer as white crystals m.p.; 156°C.

( 25 Intermediate 39

Methyl 1.2,3,4-tetrahvdro-1 -(4-hydroxvphenvl)-9H-pyridof3,4-b1indole-3carboxvlate, cis isomer

The same method but starting from racemic tryptophan ethyl ester and 4hydroxybenzaldehyde gave the title compound as pale yellow crystals ¹H NMR (DMSO) 5(ppm) ; 10.3 (s, 1H, NH-indole) 9.4 (s, 1H, OH) ; 7.8 - 7.5 (m, 8H, H aromatic); 5.1 (brs, 1H, H-1); 3.9 (m, 1H, H-3); 3.75 (s, 3H, CO₂CH₃) 3.1 (m, 1H, H-4); 2.8 (m, 1H, H-4).

AP/P/ 9 5 / 0 0 7 1 2

Intermediate 40

AP . Ο Ο 5 5 6

Methyl 1.2.3.4-tetrahydro-1-(3-hydroxy-4-methoxyphenyl)-9H-pyridof3.4blindole-3-carboxylate, cis isomer

The same method but starting from racemic tryptophan methyl ester and 3hydroxy-4-methoxybenzaldehyde gave the title compound as a yellow solid m.p.

; 140-148’C.

Intermediate 41

Methyl 1.2.3,4-tetrahvdro-1 -(4-hvdroxv-3-methoxvphenvl)-9H-pyridof3,4blindole-3-carboxylate, cis isomer

The same method but starting from racemic tryptophan methyl ester and 4hydroxy-3-methoxybenzaldehyde gave the title compound as a cream solid m.p.

; 195°C.

( <

Intermediate 42

Methyl 1,2,3,4-tetrahydro-1 -(4-ethylphenvl)-9H-pyridof3.4-b1indole-3carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 4ethylbenzaldehyde gave the cis and trans isomer of the title compound.

Cis isomer: white solid 1H NMR (CDCI3) 5(ppm): 7.65-7.1 (m, 9H, H aromatic);

5.25 (brs, 1H, H-1); 4(dd, 1H, H-3) ; 3.9 (s, 3H, CO₂CH₃); 3.4 (ddd, 1H, H-4) ,

3.1 (m, 1H, H-4); 2.7 (q, 2H, CH2CH3) 1.4 (t, 3H, CH₂CH₃).

Trans isomer: white solid m.p. : 187°C.

o

Intermediates 43 and 44

C 25 Methyl 1.2,3,4-tetrahvdro-1 -(4-isopropvlphenvl)-9H-pyridof3,4-blindole-3carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 4isopropylbenzaldehyde gave Intermediate 43. the cis isomer as a white solid 1H NMR (DMSO) 6(ppm): 10.15 (s, 1H, NH indole); 7.3-6.7 (m, 8H, H aromatic); 5 (brs, 1H, H-1); 3.6 (m, 1H, H-3) ; 3.5 (s, 3H, CO₂CH₃); 2.95-2.5 (m, 3H, H-4 + CH-(Me)₂) 2.4 (brs, 1H, NH) ; 1(d, 6H, 2XCH3) and Intermediate 44. the trans isomer as a white solid m.p. : 189°C.

Ai7f7 9 5 / 0 0 7 1 2

Intermediates 45 and 46

1233CV

Ethyl 1,2,3,4-tetrahydro-1 -(4-nitrophenyl)-9H-pvridof3,4-b1indole-3-carboxvlate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 4nitrobenzaldehyde gave Intermediate 45, the cis isomer as yellow crystals m.p.

: 168°C and Intermediate 46, the trans isomer as yellow crystals m.p. : 195“C.

Intermediate 47

Ethyl 1,2,3.4-tetrahvdro-1 -(4-dimethvlaminophenvl)-9H-pyridor3.4-b1indole-3carboxylate, mixture of cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 4dimethylaminobenzaldehyde gave the title compound as white crystals m.p. : 170°C.

(I

Intermediates 48 and 49

Ethyl 1,2,3,4-tetrahydro-1 -(3-pyridyl)-9H-pyrido[3,4-b1indole-3-carboxvlate. cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 3pyridinecarboxaldehyde gave Intermediate 48, the cis isomer as pale yellow crystals m.p. : 230-232°C and Intermediate 49, the trans isomer as white crystals m.p. : 210-214°C.

Intermediates 50 and 51

Methyl 1,2.3,4 tetrahvdro-6-fluoro-1-(3,4-methylenedioxvphenvD-9H-pvridof3.4blindole-3-carboxvlate, cis and trans isomers

The same method but starting from racemic 5-fluoro-tryptophan methyl ester and piperonal gave Intermediate 50, the cis isomer as a cream solid m.p. :60°C and Intermediate 51. the trans isomer as a cream solid m.p. : 213°C.

Intermediates 52 and 53

Methyl 1,2,3,4-tetrahydro-6-fluoro-1 -(4-methoxyphenyl )-9H-pyridor3.4-b1indole3-carboxylate, cis and trans isomers

The same method but starting from racemic 5-fluoro-tryptophan methyl ester and 4-methoxybenzaldehyde gave Intermediate 52. the cis isomer as a solid 1H NMR (CDCI3) δ (ppm) : 7.4-6.8 (m, 8H, H aromatic) ; 5.15 (brs, 1H, H-1) ; 3.9

AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6 (dd, 1 Η, Η-3) 3.8 (s, 3Η, CO2CH3) ; 3.2-2.9 (m, 2Η, Η-4) and Intermediate 53, the trans isomer as a solid m.p. : 197°C.

Intermediates 54 and 55 (1 R,3R)-Methvl 1,2.3.4-tetrahvdro-1 -(3,4-methvlenedioxvphenvl)-9H-pvridof3.4blindole-3-carboxylate, cis isomer and (1 S,3R)-methvl 1,2,3,4-tetrahydro-1 -(3.4-methylenedioxyphenyl)-9H-pvridof3,4blindole-3-carboxylate trans isomer

To a stirred solution of D-tryptophan methyl ester (11 g) and piperonal (7.9 g) in anhydrous CH2CI2 (400 mL) cooled at 0°C was added dropwise trifluoroacetic acid (7.7 mL) and the solution was allowed to react at ambient temperature. After 4 days, the yellow solution was diluted with CH2CI2 (200 mL) and washed ' with a saturated aqueous solution of NaHCO3, then with water (3x200 mL) and dried over Na2SO4. The organic layer was evaporated under reduced pressure and the residue was purified by flash chromatography eluting with dichloromethane/ethyl acetate (97/3) to give first Intermediate 54, the cis isomer (6.5 g) m.p. : 154°C followed by Intermediate 55, the trans isomer (8.4 g) m.p. : 188°C.

The following compounds were obtained in a similar manner:

Intermediate 56 q (1S, 3S) Methvl-1.2.3,4-tetrahydro-1 -(3.4-methvlenedioxvphenvl)-9H-pvridof3.4blindole-3-carboxylate, cis isomer and ( 25 (1R, 3S) methvl-1,2,3,4-tetrahydro-1-(3,4-methylenedioxvphenyl)-9H-pyridof3.4blindole-3-carboxylate, trans isomer

The same method but starting from L-tryptophan methyl ester and piperonal gave the cis and trans isomers of the title compound.

Cis isomer; white crystals m.p.: 154°C.

Trans isomer: white crystals m.p. : 187-189°C.

Intermediates 57 and 58 (1R,3R)-Methyl 1,2,3,4-tetrahvdro-1-(4-methoxvphenyl)-9H-pyridof3,4-b1indole3-carboxvlate, cis isomer and

AP/P/ 9 5 / 0 0 7 1 2

1233CV

MS,3R)-methyl 1,2,3,4-tetrahvdro-1-(4-methoxvphenvl)-9H-pyridof3,4-blindole3-carboxylate, trans isomer

The same method but starting from D-tryptophan methyl ester and 4methoxybenzaldehyde gave Intermediate 57. the cis isomer as white crystals

m.p.: 124-125°C and Intermediate 58. trans isomer as white crystals m.p.: 219222°C.

Intermediates 59 and 60

MR, 3R)-Methvl 1.2,3,4-tetrahvdro-t-(3-chloro-4-methoxyphenyl)-9H-pvridof3,410 blindole-3-carboxylate, cis isomer and (1S, 3R)-methyl 1,2,3,4-tetrahydro-1-(3-chloro-4-methoxyphenvl) 9H-pyridof3,4blindole-3-carboxylate, trans isomer ' The same method, but starting from D-tryptophan methyl ester and 3-chloro-4methoxybenzaldehyde gave Intermediate 59, the cis isomer isolated as the hydrochloride salt as white crystals m.p. : 200°C and Intermediate 60, the trans isomer as white crystals m.p.: 164°C.

intermediates 61 and 62 (1R,3R)-Methyl 1,2,3,4-tetrahvdro-1-(2,3-dihvdrobenzofbTfuran-5-vl)-9H20 pyridof3,4-b1indole-3-carboxylate, cis isomer and

MS, 3R)-methyl 1,2,3.4-tetrahvdro-1-(5-(2,3-dihydrobenzo[b1furan))-9Hpyrido[3,4-b1indole-3-carboxvlate.· trans isomer

The same method but starting from D-tryptophan methyl ester and 2,3dihydrobenzo[b]furan-5-cart>oxaldehyde gave Intermediate 61, the cis isomer as ( 25 white crystals m.p. : 282°C and Intermediate 62. the trans isomer as white crystals m.p. : 204°C.

Intermediates 63 and 64

MR.3R)-Methyl 1,2,3,4-tetrahvdro-1-(5-indanyl)-9H-pyridof3,4-b1indole-330 carboxylate cis isomer and

M S,3R)-methvl 1,2,3,4-tetrahydro-1 -(5-indanvl)-9H-pyridof3,4-blindole-3carboxylate trans isomer

The same method but starting from D-tryptophan methyl ester and indan-5carboxaldehyde gave Intermediate 63, the cis isomer as white crystals m.p. :

130-131 °C and Intermediate 64, the trans isomer as white crystals m.p. : 196^eC.

AP/P/ 9 5 / 0 0 7 1 2

1233CV

AP.00556

Intermediate 65

Ethyl 1,2,3,4-tetrahvdro-1 -(4-trifluoromethoxvphenyl)-9H-pyridof3,4-b1indole-3carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan ethyl ester and 4trifluoromethoxybenzaldehyde gave cis and trans isomers of the title compound. Cis isomer; white crystals m.p. ; 88°C.

Trans isomer: white crystals m.p. : 152°C.

... 10 Intermediate 66

Methyl 1,2,3,4-tetrahydro-1 -(5-methyl-2-thienyl)-9H-pyrido [3,4-b1indole-3. carboxylate, cis and trans isomers

The same method but starting from racemic tryptophan methyl ester and 5methyl-2-thiophenecarboxaldehyde gave the cis and trans isomers of the title compound.

Cis isomer: oily compound ¹H NMR (CDCI3) δ (ppm): 8.4 (brs, 1H, NH-indole);

7.7 - 6.6 (m, 6H, H aromatic); 5.5 (brs, 1H, H-1); 3.9 (dd, 1H, H-3); 3.85 (s, 3H, CO2CH3), 3.3 - 2.9 (m, 2H, H-4); 2.5 (s, 3H, CH3).

Trans isomer; white crystals m.p. : 194°C.

Intermediates 67 and 68 (1S.3R1-Methvl 1.2,3,4-tetrahvdro-1-(3.4-methylenedioxvphenyD-9H-pyridof3.4θ blindole-3-carboxylate and (1R,3R)-methyl 1,2,3,4-tetrahvdro-1-(3.4-methylenedioxyphenyl)-9H-pyridof3.4ζ 25 blindole-3-carboxylate

To a stirred solution of D-tryptophan methyl ester (obtained by treating the corresponding hydrochloride salt in water with saturated aqueous NaHCO3 solution and extraction with CH2CI2) (25.7g) and piperonal (19.4g) in anhydrous dichloromethane (700ml) cooled to 0°C was added dropwise trifluoroacetic acid (18.1ml) and the solution was allowed to react at 4°C. After 5 days, the yellow solution was diluted with dichloromethane (500ml). The organic layer was washed with a saturated aqueous solution of NaHCO3, then with water (3 x 500ml) until the pH was neutral and dried over Na2SO4- The organic layer was evaporated under reduced pressure to a volume of about 500ml. The trans35 isomer, which crystallised, was filtered and the filtrate was reduced to 200ml.

AP/P/ 9 5 / 0 0 7 1 2

1233CV

Another fraction of the trans-isomer crystallised. The fractions of trans-isomer were combined to give the (1S,3R) isomer, Intermediate 67, as white crystals (11.4g).

mp : 188°C ⁵ [a]g° = +32.4° (c = 1.03, CHCy.

The filtrate containing mainly the cis-isomer was reduced to 100ml and isopropyl ether (200ml) was added. Upon cooling, the (1R,3R) isomer, Intermediate 68, crystallised as a white solid (17.4g).

mp:154-155°C [a]g° = + 24.4° (c = 1.03, CHCI₃).

( Intermediate 69 (1R,3R)-Methvl 1,2,3,4-tetrahvdro-1-(3,4-methvlenedioxyphenvl)-9H-pvridof3.4blindole-3-carboxylate

Method A

Intermediate 67 (5.0g) was dissolved in methanol (150ml). Hydrogen chloride was bubbled into the solution for several minutes at 0°C and the resulting yellow solution was refluxed for 24 hours. The solvent was removed under reduced pressure and the residue was basified with a saturated aqueous solution of NaHCO3 and extracted with dichloromethane. The organic layer was washed with water, dried over Na2SO4 and purified by flash chromatography eluting with dichloromethane/methanol (99/1) to give the title compound (2.3g)

O 25 corresponding to an authentic sample of Intermediate 68.

( Method B

Intermediate 67 (25g) was heated in 1N hydrochloric acid (78.5ml) and water (400ml) at 60°C for 36 hours. From the initial pale yellow solution, a white solid precipitated. The mixture was then allowed to cool to 0°C and the solid filtered. The solid was then washed with diisopropyl ether (3 x 200ml) and dried to give the hydrochloride salt of the title compound (20g) as a white solid.

mp (dec.); 209 - 212°C

Method C

AP/P/ 9 5 / 0 0 7 1 2

1233CV

AP. Ο Ο 5 5 6

A 1 : 1 mixture of the cis and trans isomers of Intermediates 54 and 55 (2g) was heated in 1N hydrochloric acid (6.8ml) and water (15ml) at 50’C for 72 hours. A similar work-up as described in Method B above gave the hydrochloride salt of the title compound (1,7g) as a white solid.

Intermediate 70 (R)-N^a-(3.4-Methylenedioxvphenvlcarbonyl)-trvptophan methyl ester

To a suspension of D-tryptophan methyl ester hydrochloride (10.2g) in anhydrous CH2CI2 (150ml) cooled at 0°C was added dropwise triethylamine (12.3ml). To the resulting solution solid piperonyloyl chloride (8.16g) was added portionwise at the same temperature, and the mixture was stirred at room temperature for 2 h. The mixture was washed successively with water, 0.5N ® hydrochloric acid, water, a saturated aqueous solution of NaHCC>3 ^an<^ again with water. After drying over Na2SO4 and evaporation of the solvent under reduced presure, the resulting oil on trituration from hot cyclohexane afforded the title compound as a white solid (14.7g). mp: 123-124’C [α]θ^θ°= - 84.4° (c = 1.04, CHCy.

Intermediate 71 (R)-N^a-(3,4-Methvlenedioxvphenvlthiocarbonvl)-tryptophan methyl ester A mixture of Intermediate 70 (14g) and Lawesson's reagent (9.28g) in

Q dimethoxyethane (280ml) was heated at 60°C under N2 for 16 hours with stirring. The reaction mixture was evaporated to dryness and the resulting oil ζ was dissolved in ethyl acetate, then washed successively with an aqueous saturated solution of NaHCO3 ^anc* water and dried over Na2SC>4. The oily residue obtained after evaporation under reduced pressure gave, on trituration from cyclohexane, a yellow powder which was filtered and washed with cooled methanol to afford the title compound (9.74g). mp : 129-130’C [α]θ^θ°= -186.8° (c = 1.14, CHCy.

rv

Intermediate 72

1233CV (1R.3R)-Methyl 1,2,3,4-tetrahydro-1-(3,4-methvlenedioxvphenvl)-9H-pyrido[3.4blindole-3-carboxylate

A solution of Intermediate 71 (9g) and methyl iodide (10ml) in anhydrous dichloromethane (200ml) was heated at reflux under an argon atmosphere with protection from light. After 24 hours, the solvent was removed under reduced pressure to give an orange oil which on trituration from hexane gave a solid which was washed with ether and used without further purification in the next step. This compound (13.11g) was dissolved in methanol (250ml) and the solution was cooled to -78°C. NaBH4 (0.99g) was then added by portions and ^-10 the mixture was stirred at the same temperature for 1 hour. The reaction was quenched by addition of acetone (10ml) and the solvent was removed under reduced pressure. The residue was dissolved in CH2CI2, washed with water and then with brine and dried over Na2SO4- After evaporation of the solvent, the orange oil gave on trituration from a hot mixture of diethyl ether/cyclohexane an orange powder which was recrystailised from diethyl ether/pentane to afford the title compound as a pale yellow solid (5.15g) corresponding to an authentic sample of Intermediate 68.

Intermediate 73 (1 R,3R)-Methyl 1,2,3,4-tetrahydro-2-chloroacetvl-(3,4-methvlenedioxvphenvl)9H-pyrido[3.4-b1indole-3-carboxylate

Method A θ To a stirred solution of Intermediate 72 (9.7g) and NaHCO3 (2.79g) in anhydrous CHCI3 (200ml) was added dropwise chloroacetyl chloride (5.3ml) at ζ 25 0°C under N2. The resulting mixture was stirred for 1 hour at the same temperature and diluted with CHCI3 (100ml). Water (100ml) was then added dropwise with stirring to the mixture, followed by a saturated aqueous solution of NaHCO3- The organic layer was washed with water until neutrality and dried over Na2SO4- After evaporation of the solvent under reduced pressure, the oily compound obtained was crystallised from ether to give the title compound as a pale yellow solid (9.95g). mp : 233°C (a]g°°=- 125.4° (c= 1.17, CHCy.

Method B

AP .0 0 5 5 6 f <

Q

C 25

Chloroacetyl chloride (4ml) was added dropwide to a solution of Intermediate 72 (16.1g) and triethylamine (7ml) in anhydrous CH2CI2 (200ml) at 0°C under N2 The solution was stirred at 0°C for 30 minutes, then diluted with CH2CI2 (300ml). The solution was washed with water (200ml), a saturated aqueous solution of NaHCO3 (300ml) and brine (400ml). After drying over Na2SO4 and evaporation under reduced pressure, the resulting solid was washed with ether (300ml) to give the title compound as a pale yellow solid (18.3g).

Intermediate 74

Methyl 1,2.3.4-tetrahvdro-6-methyl-1 -(3,4-methvlenedioxyphenvn-9H-pyridof3.4blindole-3-carboxylate. cis and trans isomers

The cis and trans isomers of the title compound were prepared using the method described in Intermediate 1 but starting from racemic 5-methyltryptophan methyl ester and piperonal.

Cis isomer; yellow solid m.p. : 85°C.

Trans isomer: yellow solid m.p.; 185°C.

Intermediates 75 and 76 (1R. 3R)-Methyl 1.2,3,4-tetrahydro-1-(7-(4-methvl-3,4-dihvdro-2Hbenzo[1.41oxaz)nvl))-9H-pvrido[3.4-b1indole-3-carboxvlate, cis isomer and (1S,

3R)-Methvl 1,2,3,4-tetrahydro-1 -(7-(4-methyl-3,4-dihvdro-2Hbenzofl ,41oxazinvl))-9H-pyridof3.4-b1indole-3-carboxvlate, trans isomer The same method, as described for intermediates 54 and 55, but starting from D-tryptophan methyl ester and 4-methyl-3,4-dihydro-2H-benzo[1,4]oxazine-7carboxaldehyde gave Intermediate 75 the cis isomer as an oily compound ¹H NMR (CDCI3) δ (ppm); 7.6-7.1 (m, 5H); 6.9-6.6 (m, 3H); 5.15 (br s, 1H); 4.3 (t,

2H); 4 (dd, 1H); 3.8 (s, 3H); 3.3 (t, 2H); 3.3-2.95 (m, 2H); 2.9 (s, 3H); 1.6 (br s) and intermediate 76, the trans isomer as white crystals m.p. : 119-121’C.

Intermediate 77

Methyl 1,2,3,4-tetrahydro-1 -(5-(N-benzylindolinyl))-9H-pyridof3.4-blindole-3carboxvlate, mixture of (1R, 3R) and (1S, 3R) isomers

The same method, as described for intermediates 54 and 55, but starting from

D-tryptophan methyl ester and N-benzylindoline-5-carboxaldehyde gave intermediate 77 as an oily compound.______

AP/P/ 9 5 / 0 0 7 1 2

1233CV

Intermediates 78 and 79 (1R, 3R)-Methyl 1,2,3.4-tetrahvdro-1-(4-carbomethoxyphenyl)-9H-pyridoi3.4blindole-3-carboxylate, cis isomer and (1S, 3R)-methvl 1.2,3,4-tetrahvdro-1-(45 carbomethoxvphenvl)-9H-pyridor3.4-b1indole-3-carboxvlate, trans isomer

The same method, as described for intermediates 54 and 55, but starting from D-tryptophan methyl ester and methyl 4-formylbenzoate gave Intermediate 78, the cis isomer as white crystals m.p. : 157-160°C and Intermediate 79, the trans isomer as pale yellow crystals m.p. : 124-126°C.

Intermediate 80 (1R, 3R)-Methyl 1.2,3,4-tetrahydro-2-f2-(benzyloxycarbonyl)-R-prolvn-1 -(3,4methylenedioxvphenvl)-9H-pvridoi3,4-blindole-3-carboxylate

A solution of N-(benzyloxycarbonyl)-D-proline acid chloride (0.64 g, 2.4 mmol) in anhydrous dichloromethane (10 mL) was added dropwise to a stirred solution of intermediate 54 (0.7 g, 2 mmol) and triethylamine (0.33 mL, 2.4 mmol) in dichloromethane (15 mL) at - 10°C. The mixture was stirred for 2 h at - 10°C after which it was diluted with dichloromethane (50 mL), washed with '20 hydrochloric acid (1N), water, a saturated solution of NaHCO3, a saturated NaCl solution and dried over Na₂SO₄. Evaporation of the solvent and recrystallisation of the crude product from methanol gave the title compound as pale yellow θ crystals (0.75 g) m.p. . 268-270°C.

ζ 25 Intermediate 81 (1R, 3R)-Methyl 1,2,3,4-tetrahvdro-2-i2-(benzvloxvcarbonvl)-S-prolyl1-1-(3,4methylenedioxyphenyl)-9H-pvrido[3,4-blindole-3-carboxylate A solution of N-(benzyloxycarbonyl)-L-proline acid chloride (0.86 g, 3.2 mmol) in anhydrous dichloromethane (10 mL) was added dropwise to a stirred solution of intermediate 54 (0.91 g, 2.6 mmol) and triethylamine (0.44 mL, 3.2 mmol) in dichloromethane (20 mL) at - 10°C. The mixture was stirred for 2 hours at - 10°C after which it was diluted with dichloromethane (60 mL), washed with hydrochloric acid (1N), water , a saturated solution of NaHCO_s, a saturated NaCl solution and dried over Na₂SO₄. Evaporation of the solvent and

AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6 recrystallisation of the crude product from methanol/water gave the title compound as pale yellow crystals (0.8 g) m.p.: 115-120^eC.

Intermediate 82 (1R, 3R1-Methvl 1.2.3.4-tetrahvdro-2-(2-chloropropionvl)-1-(3.4methvlenedioxyphenvl)-9H-pvridof3.4-b1indole-3-carboxvlate

To a solution of (S)-(-)-2-chloropropionic acid (87 μΙ, 1 mmol) in anhydrous 10 dichloromethane (15 mL), was added dicyclohexylcarbodiimide (0.23 g,

l. 1 mmol). Intermediate 54 (0,35 g, 1 mmol) was then added and the mixture ( was stirred at room temperature for 20 hours. The formed precipitate of dicyclohexylurea was removed by filtration, the filtrate was evaporated in vacuo and the crude product was purified by flash chromatography eluting with toluene/ethyl acetate : 95/5. The oily compound obtained was then crystallised from ether/hexane to give the title compound as pale yellow crystals (0.31 g)

m. p. : 125-127’C.

Intermediate 83 (1R, 3R)-Methvl 1.2.3.4-tetrahvdro-2-(2-chloropropionvl)-1 -(3,4methvlenedioxvphenvO-9H-pvrido[3.4-b1indole-3-carboxvlate To a solution of (R)-(+)-2-chloropropionic acid (191 μΙ, 2.2 mmol) in anhydrous dichloromethane (30 mL), was added dicyclohexylcarbodiimide (0.45 g,

2.2. mol). Intermediate 54 (0,7 g, 2 mmol) was then added and the mixture was / 25 stirred at room temperature for 20 hours. The formed precipitate of dicyclohexylurea was removed by filtration, the filtrate was evaporated in vacuo and the crude product was purified by flash chromatography eluting with toluene/ethyl acetate : 95/5. The oily compound obtained was then crystallised from ether/hexane to give the title compound as pale yellow crystals (0.74 g)

m.p. : 126-128’C.

Intermediates 84 and 85 f 1R, 3R)-Methvl 1.2,3,4-tetrahvdro-1 -(3.4-dibenzvloxyphenvl)-9H-pyridoi3.4blindole-3-carboxvlate cis isomer and (1S, 3R)-methvl 1,2,3,4-tetrahvdro-1-(3,435 dibenzvloxvphenvl)-9H-pyrido i3.4-blindole-3-carboxvlate trans isomer

AP/P/ 9 5 / 0 0 7 1 2

1233CV

The same method as described for intermediates 54 and 55 but starting from Dtryptophan methyl ester and 3,4-dibenzyloxybenzaldehyde gave intermediate 84, the cis isomer as an oily compound 1H NMR (CDCI3) 5(ppm): 7.5 - 6.95 (m, 15H) ; 6.85 (s, 1H) ; 6.75 (s, 2H) ; 5.1 (s, 2H) ; 5 (br s, 1H) ; 4.95 (d, 2H) 3.85 (dd, 1H); 3.7 (s, 3H); 3.2-2.8 (m, 2H); 2.3 (br s, 1H) and intermediate 85, the trans isomer as an oily compound ¹HNMR (CDCI3) δ (ppm) 7.6-7 (m, 15H); 6.96.7 (m, 3H) ; 5.2 (br s, 1H) ; 5.1 (s, 2H) ; 5 (s, 2H) ; 3.8 (t, 1H) ; 3.65 (s, 3H) ; 3.3-3 (m, 2H); 2.25 (br s, 1H).

Intermediate 86 (6R, 12aR)-2,3,6.7.12.12a-Hexahvdro-6-(3.4-dibenzvloxyphenyl)-2-methvlρνΓ3ζΐηοΓ2', T : 6, npyrido[3.4-b1indole-1.4-dione

The same two step procedure but starting from intermediate 84 and methylamine gave, after recrystallisation from dichloromethane/ether, the title compound as white crystals m.p. : 158-160’C, [a]²⁰°_D = + 11.7° (c = 1.23 ; CHCI3).

Intermediate 87

Methyl 1.2.3.4-tetrahvdro-1 -(5-(2-methvlisoindolinv0)-9H-pvrido[3.4-b1indole-3carboxvlate, mixture of (1R.3R) and (1S.3R) isomers

The same method, as described for intermediates 54 and 55, but starting from D-tryptophan methyl ester and N-methylisoindoline-5-carboxaldehyde gave intermediate 87 as an oily compound.

Example 1

Cis-2.3.6.7.12.12a-hexahvdro-2-methvl-6-(3.4-methylenedioxvphenvl)PvrazinorZ.rO.npyridofSAblindole -1.4-dione

a) To a stirred solution of intermediate 1 (2 g) and NaHCO3 (0.6 g) in anhydrous 30 CHCI3 (40 mL) was added dropwise chloroacetyl chloride (1.1 mL) at O’C.

The resulting mixture was stirred for 1 hour at the same temperature and diluted with CHCI3. Water (20 mL) was then added dropwise with stirring to the mixture, followed by a saturated solution of NaHCO3- The organic layer was washed with water until neutrality and dried over Na2SO4. After evaporation of the solvent under reduced pressure, cis-methyl 1,2,3,4AP/P/ 9 5 / 0 0 7 1J

AP . Ο Ο 5 5 6 tetrahvdro-2-chloroacetvl-1-(3,4-methvlenedioxvphenvl)-9H-pyridof3,4blindole-3-carboxvlate was obtained as an oil which was crystallised from ether (2 g, m.p. : 215-218°C) and was used without further purification in the next step.

b) To a stirred suspension of the chloroacetyl intermediate (0.34 g) in MeOH (20 mL) was added at ambient temperature a solution of methylamine (33% in EtOH) (0.37 mL) and the resulting mixture was heated at 50°C under N2 for 14 hours. The solvent was removed under reduced pressure and the residue was dissolved in CH2CI2 (50 mL). After washing with water (3x30 mL), drying over Na2SO4 and evaporating to dryness, the residue was purified by flash chromatography eluting with CH2Cl2/MeOH (99/1) and recrystallised from MeOH to give the title compound as white crystals (0.19 g) m.p. : 253-255’C.

Analysis for C22HigN3O4_:

Calculated;C,67.86;H,4.92;N, 10.79;

Found:C,67.53;H,4.99;N, 10.62%.

The following compounds were obtained in a similar manner;

Example 2

Cis-2, 3, 6, 7, 12, 12a-hexahydro-2-butyl-10-fluoro-€-(4-methoxvphenyQpvrazinof2', Γ : 6.11pyrido r3,4-b1indole-1.4-dione

The same two step procedure but starting from butylamine and intermediate 52 gave, after recrystallisation from ethanol, the title compound as white crystals m.p. ; 182°C.

Analysis for C25H26FN3O3 (0.1 H2O);

Calculated : C, 68.67 ; H, 6.04 ; N, 9.61;

Found ; C, 68.38 ; H, 6.11 ; N, 9.53%.

Example 3

Trans-2,3,6,7,12,12a-hexahydro-2-methvl-6-(3.4-methylenedioxyphenyl)pvrazinof2^l.1':6,11pyridof3.4-b1indole -1,4-dione

AP/P/ 95/00711

1233CV

The same two step procedure but starting from methylamine and intermediate 2 gave, after recrystallisation from toluene, the title compound as white crystals m.p. : 301-303’C.

Analysis for C22H19N3O4 5 Calculated; C,67.86;H,4.92;N, 10.79;

Found:C,67.98;H,4.98;N, 10.73%.

Example 4

Cis-2^3.6,7,12,12a-hexahydro-6-(3,4-methylenedioxyphenvl)10 pyrazino^'.rO.npyridofS^-blindole -1.4-dione

The same two step procedure but starting from ammonia and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals f m.p. ; 283-285’C.

Analysis for C21 H-j 7N3O4

Calculated: C,67.19;H,4.56;N,11.19;

Found: C,67.04;H,4.49;N, 11.10%.

Example 5

Cis-2.3.6,7,12,12a-hexahydro-10-fluoro-6-(4-methoxyphenyl)-2-(2.2.220 trifluoroethvl)-pyrazinof2'.1': 6,npyrido 13,4-b1indole-1.4-dione

The same two step procedure but starting from 2,2,2-trifluoroethylamine and intermediate 52 gave, after recrystallisation from ethanol/diisopropyl ether, the title compound as white crystals m.p.: 190^eC.

Analysis for C23H19F4N3O3: r 25 Calculated ; C, 59.87 ; H, 4.15 ; N, 9.11;

Found ; C, 59.81 ; H, 4.18 ; N, 9.21%.

Example 6

Cis-2.3.6.7.12.12a-hexahvdro-10-fluoro-2-methvl-6-(3,4-methvlenedioxyphenvn30 pyrazinore'.T : 6.11pyridof3.4-b1indole-1,4-dione

The same two step procedure but starting from methylamine and intermediate 50 gave, after recrystallisation from ethanol, the title compound as white crystals m.p. ; 292’C.

Analysis for C22H18FN3O4

Calculated ; C, 64.86 ; H, 4.45 ; N, 10.31; AP/P/ 9 5 / 0 0 7 1 2

AP . Ο Ο 5 5 6

Found . C, 64.66 ; Η, 4.60 ; Ν, 10.21%.

Example 7 (6R, 12a$)-2.3.6.7.12,12a-hexahvdro-2-methvl-6-(3.4-methvlenedioxvohenvl)5 pvrazinof2',r : 6.1lpyridof3.4-b1indole-1,4-dione

The same two step procedure but starting from methylamine and the trans isomer of intermediate 56 gave, after recrystallisation from toluene, the title compound as white crystals m.p. :287-289’C.

Analysis for C22H19N3O4 (0.25 toluene);

_ 10 Calculated : C, 69.16 ; H, 5.13 ; N, 10.19;

Found ; C,69.09 ; H, 5.14 ; N, 10.19%.

20° f > [a]_D = -293.4’ (C=1.28; CHCy.

o

Example 8 (6S, 12aR)-2.3.6.7,12,12a-hexahydro-2-methvl-6-(3.4-methvlenedioxyphenvl)pyrazino f2\ T : 6.11pyridof3,4-blindole-1,4-dione

The same two step procedure but starting from methylamine and intermediate 55 gave, after recrystallisation from toluene, the title compound as white crystals m.p.; 287°C.

Analysis for C22H19N3O4 (0.3 toluene);

Calculated ; C, 69.41 ; H, 5.17 ; N,· 10.08;

Found : C, 69.56 ; H.5.24 ; N, 10.08%.

20° [a]_D = + 297.9’ (C=1.21; CHClg).

Vi tv>

Example 9

Cis-2, 3, 6, 7, 12, 12a-hexahvdro-2-f2-(2-ovridyl)-ethvn-6-(3.4methvlenedioxvphenvl)-pvΓazinof2^,, 1 '-6,11ovridof3.4-b1indole-1,4-dione The same two step procedure but starting from 2-(2-pyridyl)ethylamine and intermediate 1 gave, after recrystallisation from 2-propanol, the title compound as white crystals m.p.: 218-222’C.

Analysis for 028^24^04;

Calculated ; C, 69.99 ; H, 5.03 ; N, 11.66;

Found : C, 69.92 ; H, 5.16 ; N, 11.48%.________

1233CV

Example 10

Cis-2,3,6,7,12.12a-hexahvdro-2-(2-pyridvlmethvn-6-(3.4methylenedioxyphenyl)-pyrazinof2^l, 1': 6J1pyridof3.4-b1indole-1.4-dione

The same two step procedure but starting from 2-pyridylmethylamine and intermediate 1 gave, after recrystallisation from DMF/water, the title compound as cream crystals m.p : 285-286’C.

Analysis for C77H92N4O4 (0.4 H₂O):

Calculated : C, 68.46 ; H.4.85 ; N, 11.83;

Found : C, 68.58 ; H, 4.88 ; N, 11.90%.

Example 11 ¹ Cis-2,3,6,7,12,12a-hexahvdro-2-(3-pyridylmethvl)-6-(3,4methvlenedioχyphenvl)-pyrazinof2^,. 1’: 6,11pyridof3.4-b1indole-1.4-dione 15 The same two step procedure but starting from 3-pyridylmethylamine intermediate 1 gave, after recrystallisation from CH₂CI₂/MeOH, the compound as cream crystals m.p. : 292-293°C.

Analysis : C27H22N4O4:

Calculated ; C, 69.52 ; H, 4.75 ; N, 12.01;

Found : C, 69.27 ; H, 4.74 ; N, 11.37%.

Example 12 q Cis-2.3.6.7.12.12a-hexahydro-2-(4-pyridylmethvl)-6-(3.4methvlenedioxvphenv0-pyrazinof2^l, Γ : 6,11pyridoi3,4-b1indole-1,4-dione ( 25 The same two step procedure but starting from 4-pyridylmethylamine intermediate 1 gave, after recrystallisation from MeOH, the title compound as pale yellow crystals m.p.; 273-274°C.

Analysis for C₂7H₂₂N4O4 (1.8 H₂O):

Calculated : C, 65.00 ; H, 5.17 ; N, 11.23;

Found . C, 65.11 ; H, 4.85 ; N, 11.07%.

Example 13

Cis-2.3,6.7.12.12a-hexahvdro-2-ethvl-6-(3,4-methvlenedioxyphenyl)pyrazinof2^,.r:6,npyridoi3.4-b1indole -1,4-dione and title >

<0 cn and ro

AP.00556

The same two step procedure but starting from ethylamine and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 272-274’C.

Analysis for C23H21N3O4 5 Calculated: C,68.47,Ή,5.25;N, 10.42;

Found;C,68.52;H,5.35;N,10.53%.

Example 14

Cis-2,3,6,7,12.12a-hexahydro-2-(2,2,2-trifluoroethvl)-6-(3,4- 10 methylenedioxvphenvl)-pyraz^nof2^,. 1 ’:6,11ovridof3.4-b|indole -1,4-dione

The same two step procedure but starting from 2,2.2-trifluoroethylamine and _f intermediate 1 gave, after recrystallisation from EtOH, the title compound as white crystals m.p. : 303^eC.

Analysis for C23H18F3N3O4:

Calculated: C,60.40;H,3.97;N,9.19;

Found;C,60.43;H,4.15;N,9.16%.

Example 15

Cis-2.3.6.7.12,12a-hexahydro-6-(3,4-methvlenedioxvphenyl)-2-propyl20 pyrazinof2^,,1^,:6,npyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from propylamine and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals

Q m.p. :270-271 °C.

Analysis for C24H23N3O4.

(· 25 Calculated; C,69.05;H,5.55;N,10.07;

Found:C,69.22;H,5.50;N,9.80%.

Example 16

Cis-2.3,6,7.12.12a-hexahydro-2-isopropyl-6-(3,4-methylenedioxvohenvl)30 pyrazinore*, 1 ':6,11ovridof3.4-b1indole -1,4-dione

The same two step procedure but starting from isopropylamine and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals m.p.. 248-250°C.

Analysis for C24H23N3O4:

Calculated: C,69.05;H,5.55;N, 10.07; ----AP/r/ 9 5 / 0 0 7 1 2

1233CV

Found:C,68.86;H,5.66;N,10.21%.

Example 17

Cis-2.3,6,7,12,12a-hexahvdro-2-cyclopropvl-6-(3.4-methvlenedioxyphenvl)5 pyrazinof2', 1 *:6.11pyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylamine and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 290-292’C.

Analysis for C24H21N3O4:

Calculated: C,69.39;H,5.10;N,10.11;

Found:C,69.11;H,5.20;N,9.94%.

Example 18

Cis-2.3.6.7,12.12a-hexahvdro-2-butyl-6-(3.4-methvlenedioxvohenvD15 pvrazinof2^,.r:6.npyridof3.4-b1indole -1.4-dione

The same two step procedure but starting from butylamine and intermediate 1 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p.: 241-243^eC.

Analysis for C25H25N3O4:

Calculated: C,69.59;H,5.84;N,9.74;

Found:C,69.77;H,5.82;N,9.81 %.

, Example 19

T rans-2.3.6.7.12.12a-hexahvdro-2-butvl-6-(3.4-methvlenedioxyphenyl).... 25 ovrazino^'.TO.IIovridofS^-blindole -1,4-dione ~ The same two step procedure but starting from butylamine and intermediate 2 gave, after recrystallisation from toluene, the title compound as white crystals m.p.: 243’C.

Analysis for C25H25N3O4:

Calculated: C,69.59;H,5.84;N_I9.74;

Found:C,69.80;H,5.78;N,9.52%.

Example 20

Cis-2.3.6.7,12,12a-hexahydro-2-cvclopropylmethvl-6-(3,435 methylenedioxvphenvl)-pyrazinof2^,,r:6,noyridof3.4-b1indole -1.4-dione

AP/P/ 95/0071J

AP. Ο Ο 5 5 6

The same two step procedure but starting from cyclopropylmethylamine and intermediate 1 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 217-218°C.

Analysis for C25H23N3O4:

Calculated; C,69.92;H,5.40;N,9.78;

Found;C,70.02,H,5.47,N,9.84%.

Example 21

Cis-2.3.6,7,12.12a-hexahvdro-2-cvclopentyl-6-(3.4-methvlenedioxvphenyl)10 pvrazinof2^l,r.6.npyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from cyclopentylamine and intermediate 1 gave, after recrystallisation from acetone, the title compound as ' white crystals m.p.; 270^eC.

Analysis for C26H25N3O4:

Calculated; C,70.41 ;H,5.68;N,9.47;

Found;C,70.58,H,5.63;N,9.38%.

Example 22

Cis-2.3,6,7,12,12a-hexahvdro-2-cvclohexyl-6-(3.4-methvlenedioxvphenvl)20 pvrazinof2',1'.e,1lpvridof3,4-b1indole -1,4-dione

The same two step procedure but starting from cyclohexylamine and intermediate 1 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p.; 268-269°C.

Analysis for C27H27N3O4.

Calculated. C,70.88;H,5.95;N,9.18;

Found;C,70.82;H,5.89;N,9.21 %.

Example 23

Cis-2,3,6,7.12,12a-hexahvdro-2-benzvl-6-(3.4-methvlenedioxyphenvl)30 pyrazinof2'.r:e,npvridof3.4-b1indole -1,4-dione

The same two step procedure but starting from benzylamine and intermediate 1 gave, after recrystallisation from dichloromethane/hexane, the title compound as white crystals m.p.; 285-287°C.

Analysis for ¢28^23^304(1 H2O);

Calculated; C,69.55,Ή,5.21 ;N,8.69; -----AP/P/ 9 5 / 0 0 7 172

1233CV

Found:C,69.30;H,5.06;N,8.48%.

Example 24

Cis-2.3.6.7,12.12a-hexahydro-2-(4-fluorobenzvl)-6-f3.4-methylenedioxyphenyl)5 pvrazinof2^l, Γ:6.11pyrtdof3.4-b1indole -1.4-dione

The same two step procedure but starting from 4-fluorobenzylamine and intermediate 1 gave, after recrystallisation from acetone, the title compound as white crystals m.p.: 281-283’C.

Analysis for 02βΗ22^ΡΝ3θ4^:

Calculated. C,69.56;H,4.59;F,3.93;N,8.69;

Found:C69.54;H,4.58;F,3.82;N,8.63%.

Example 25

Cis-2.3.6.7,12.12a-hexahvdro-6-(4-methoxyphenvD-2-methyl15 pyrazinof2', 1 ':6.11pyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 3 gave, after recrystallisation from 2-propanol, the title compound as white crystals m.p. : 257-263’C.

Analysis for C₂2H₂1N3O3:

Calculated: C,70.38;H,5.64;N,11.19;

Found:C,70.11;H,5.55;N,11.15%.

Example 26

Trans-2.3.6,7.12,12a-hexahvdro-6-(4-methoxyphenyQ-2-methyl25 pvrazinof2^,.1^,:6.npyrido[3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 4 gave, after recrystallisation from diisopropyl ether, the title compound as white crystals m.p. : 225-228’C.

Analysis for ΰ22^21^3θ3^:

Calculated: C,70.38;H,5.64;N,11.19;

Found:C,70.34;H,5.77;N, 11.19%.

Example 27

Cis-2,3,6.7.12.12a-hexahydro-2-ethyl-6-(4-methoxyphenvl)35 pyrazinof2', 1 '.6,11pyridof3,4-blindole -1,4-dione

AP/P/ 9 5 / 0 0 7 1 2

AP.00556

The same two step procedure but starting from ethylamine and intermediate 3 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 245-255’C.

Analysis for C23H23N3O3:

Calculated: C,7O.93;H,5.95;N, 10.79;

Found:C,70.74;H,6.06;N,10.87%.

Example 28

Cis-2.3.6.7,12,12a-hexahydro-6-(4-methoxvohenyl)-2-(2.2.210 trifluoroethynpvrazinof2^,.r:6.1lovridof3.4-blindole -1,4-dione

The same two step procedure but starting from 2,2,2-trifluoroethylamine and intermediate 3 gave, after recrystallisation from ethanol , the title compound as white crystals m.p. : 232°C.

Analysis for C23H20F3N3O3:

Calculated: C,62.30;H,4.55;N,9.48;

Found:C,62.08;H,4.66;N,9.54%.

Example 29

C is-2.3,6,7.12,12a-hexahydro-2-buty 1-6-( 4-methoxvohenyl)20 pyrazinof2'₁1 ':6,11ovridof3,4-b1indole -1.4-dione

The same two step procedure but starting from butylamine and intermediate 3 gave, after recrystallisation from methanol,the title compound as white crystals m.p. : 157°C.

Analysis for C₂5H₂7N3O3(0.5H₂O):

Calculated: C,70.40;H,6.62;N,9.85;

Found:C,70.25;H,6.60;N,9.83%.

Example 30

Trans-2,3.6.7,12,12a-hexahydro-2-butvl-6-(4-methoxyphenyl)30 pvrazinof2^,.1':6.npyridof3,4-blindole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 4 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 212-214’C.

AP/P/ 9 5 / 0 0 7 1 2

1233CV

Found:C,71.81;H,6.55;N,10.03%.

Example 31

Cis-2.3.6.7.12.12a-hexahvdro-6-(4-methoxvphenyl)-2-cyclopropylmethyl5 pyrazinof2', Γ:6.11pyridof3,4-b1indole -1.4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 3 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 180-185’C.

Analysis for C25H25N3O3 (O.5H2O):

Calculated: C,70.74;H,6.17;N,9.90;

Found:C, 70.91 ; H, 6.16 ; N, 9.80%.

f ' Example 32

Cis-2,3,6,7,12,12a-hexahvdro-2-benzvl-6-(4-methoxyphenv015 pvrazinof2^,,r:6,npvrido[3.4-b1indole -1,4-dione

The same two step procedure but starting from benzylamine and intermediate 3 gave, after recrystallisation from acetone, the title compound as white crystals m.p.: 275-279*C.

Analysis for C28H25N3O3:

Calculated: C,74.48;H,5.58;N,9.31;

Found:C,74.53;H,5.60;N,9.20%.

s Example 33

Cis-2.3.6,7,12,12a-hexahvdro-6-(3-methoxyphenyt)-2-methyl( 25 pvrazinof2^l,r:6.npvrido[3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 5 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 267-269’C.

Analysis for C22H21N3O3:

Calculated: C,70.38;H,5.64;N,11.19;

Found:C,70.32,H,5.59;N, 11.25%.

Example 34

Cis-2.3.6,7,12,12a-hexahydro-6-(4-ethoxyphenvn-2-methvl35 pyrazinof2', 1 ':6.11pyridof3,4-blindole -1,4-dione

AP.00556

The same two step procedure but starting from methylamine and intermediate 6 gave, after recrystallisation from methanol, the title compound as white crystals m.p. ; 247-248’C.

Analysis for C23H23N3O3.

Calculated: C,70.93.H,5.95;N, 10.79;

Found:C,71.23;H,5.95;N,10.63%.

Example 35

Cis-2,3,6.7,12,12a-hexahydro-6-(4-ethoxyphenyl)-2-cyclopropvlmethvl10 pvrazinof^.r^.npyridoO^-blindole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and θ intermediate 6 gave, after recrystallisation from 2-propanol, the title compound as white crystals m.p.: 160-162’C.

Analysis for 02θΗ27^3θ3^:

Calculated: C,72.71 ;H,6.34;N,9.78;

Found:C,72.28;H,6.39;N,9.71 %.

Example 36

C is-2,3,6.7,12,12a-hexahydro-6-( 2,3-di h vdrobenzof blf uran-5-vl )-2-methvl20 pvrazinof2^l.r:6,11pvrido[3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 8 gave, after recrystallisation from methanol, the title compound as white crystals 0 m.p.: 292-294’C.

Analysis for C23H21N3O3:

( 25 Calculated: C,71.30;H,5.46;N, 10.85;

Found:C,71.15;H,5.56;N,10.84%.

Example 37

Cis-2.3.6.7.12,12a-hexahvdro-6-(2,3-dihvdrobenzofbTfuran-5-vl)-230 cvclopropylmethvl-pvrazinof2'· T;6.11pyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 8 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 165-166°C.

Analysis for C26H25N3O3:

Calculated: C,73.05;H,5.89;N,9.83;-----—

AP/P/ 9 5 / 0 0 7 12

1233CV

Found:C,73.08;H,5.97;N,9.87%.

Example 38

Cis-2.3.6.7,12.12a-hexahydro-6-(3.4-ethvlenedioxvohenvl)-2-nnethyl5 pyrazinof2', 1 ':6,1Ipyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 10 gave, after recrystallisation from acetone, the title compound as white crystals m.p.: 303-305°C.

Analysis for C23H21N3O4:

Calculated: C,68.47;H,5.25;N,10.42;

Found:C,68.35;H, 5.31 ;N, 10.27%.

I

Example 39

Cis-2.3,6.7,12,12a-hexahvdro-6-(3.4-ethvlenedioxyphenv0-2-cvclopropylmethvl15 ovrazinof2^l, 1 ':6, 11ovridof3.4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 10 gave, after recrystallisation from dichloromethane/ether, the title compound as white crystals m.p.: 288-290’C.

Analysis for C26H25N3O4:

Calculated. C,70.41 ;H,5.68;N,9.47,

Found:C,70.15;H,5.62;N,9.30%.

(j Example 40

Cis-2,3,6.7,12.12a-hexahydro-2-butvl-6-(2-chlorophenvl)( 25 pvrazinof2'.r:6.novridof3.4-b1indole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 12 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p.: 146^eC.

Analysis for C24H24CIN302(0.75 H2O):

Calculated: C,66.20;H,5.90;N,9.65;

Found:C,66.15;H,5.95;N,9.69%.

Example 41

Cis-2.3.6.7.12,12a-hexahydro-6-(4-chlorophenvl)-2-methvl35 pvrazinof2^,.r:6.npyridof3.4-blindole -1,4-dione

AP/P/ 9 5 / 0 0 7 1 2

AP.0 0 5 5 6

The same two step procedure but starting from methylamine and intermediate 13 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 274°C.

Analysis for C2iH<|gCIN3O2 (0.25 H2O).

Calculated; C,65.63;H,4.85;N,10.93;

Found;C,65.39;H,4.84;N,10.85%.

Example 42

Cis-2,3.6,7,12.12a-hexahvdro-2-butvl-6-(4-chlorophenvl)10 Pvrazinof2^,.r:6,1lpvridof3,4-b1indole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 13 gave, after recrystallisation from ethanol/water, the title compound as white crystals m.p. : 164-166’C.

Analysis for C24H24CIN3O2:

Calculated: C,68 32;H,5.73;CI,8.40;N,9.96;

Found:C,68.48;H,5.64;CI,8.37;N,9.99%.

Example 43

Cis-2,3,6,7,12,12a-hexahvdro-6-(3.4-dichlorophenyl)-2-methyl20 pvrazinof2', 1':6,1 lpvridof3,4-blindole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 15 gave, after recrystallisation from ethanol/DMF, the title , compound as white crystals m.p.: >260’C.

Analysis for C21H-, 7CI2N3O2 (0.5 H2O):

( 25 Calculated: C,59.39;H,4.29;N,9.93;

Found:C,59.32;H,4.16;N,9.99%.

Example 44

Cis-2,3.6,7.12,12a-hexahvdro-2-butvl-6-phenvl-pvrazinof2^l, 1 ':6,11pyridof3.430 blindole -1,4-dione

The same two step procedure but starting from butylamine and cis-methyl 1 ^.SAtetrahydro-l-phenyl-OH-pyridoIS^-bJindole-S-carboxylate¹ gave, after recrystallisation from methanol/water, the title compound as white crystals m.p. : 243-245’C.

Analysis for C24H25N3O2: ______-_-_.

ap/p; 9 5 / o u / 1 z

1233CV

Calculated: C,74.39;H,6.50;N,10.84;

Found:C,74.54;H,6.51 ;N,10.86%.

1. D. Soerens et al.. J. Org. Chem. 44, 535 - 545 (1979).

Example 45

Cis-2.3.6.7,12,12a-hexahvdro-2-benzyl-6-phenyl-pyrazinof2', 1 '6,11ovridof3.4blindole -1.4-dipne

The same two step procedure but starting from benzylamine and cis-methyl1,2,3,4-tetrahydro-1 -phenyl-9H-pyrido[3,4-b]indole-3-carboxylate gave, after 10 recrystallisation from methanol, the title compound as white crystals m.p. : 193- 195^eC.

Analysis for C27H23N3O2:

Calculated: C,76.94;H,5.50;N,9.97;

Found:C,77.23;H,5.54;N,9.97%.

Example 46

Trans-2.3.6,7,12.12a-hexahydro-2-benzvl-6-phenvl-pvrazinor2', T:6,11ovrido[3.4blindole -1,4-dione

The same two step procedure but starting from benzylamine and cis-methyl20 1,2,3,4-tetrahydro-1-phenyl-9H-pyrido[3,4-b]indole-3-carboxylate gave, after recrystallisation from methanol, the title compound as white crystals m.p. :

284^eC.

Analysis for C27H23N3O2:

Calculated: C,76.94,H,5.50;N,9.97;

c 25 Found: C,76.88; H, 5.45; N,9.89%.

Example 47

Cis-2,3,6.7,12,12a-hexahvdro-2-methyl-6-( 1.2.3,4-tetrahydro-6-naphthv0pvrazinof2^l,T:6,11pvridof3.4-b1indole -1,4-dione 30 The same two step procedure but starting from methylamine and intermediate 17 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: >260°C.

Analysis for C25H25N3O2

Calculated: C,75.16;H,6.31;N, 10.52;

Found:C,74.93;H,6.43;N, 10.63%.-----------------—·

AP/P/ 95/00712

1233CV

AP. Ο Ο 5 5 6

Example 48

Cis-2.3.6.7.12,12a-hexahvdro-2-isoproovl-6-(1.2,3.4-tetrahvdro-6-naphthvl)pyrazinore¹, Γ:6,11pyridof3,4-b1indole -1.4-dione 5 The same two step procedure but starting from isopropylamine and intermediate gave, after recrystallisation from the title compound as off-white crystals m.p. : 244-246’C.

Analysis for C27H29N3O2 (O.25H2O):

Calculated: C,75.06;H,6.88;N,9.73;

_c 10 Found:C,75.00;H,6.83;N,9.69%.

Example 49

Cis-2,3.6.7.12,12a-hexahydro-2-cyclopropylmethyl-6-( 1,2,3.4-tetrahydro-6naphthylD-pyrazinofZ, 1 ':6,1 loyridof3.4-blindole -1,4-dione 15 The same two step procedure but starting from cyclopropylmethylamine and intermediate 17 gave, after recrystallisation from ethanol/pentane, the title compound as white crystals m.p. : 125’C.

Analysis for C28H29N3O2 (0.25 H2O):

Calculated: C,75.73;H,6.70;N,9.46;

Found:C,75.45;H,6.86;N,9.14%.

Example 50

C is-2,3,6.7.12.12a-hexahydro-2-methyl-6-(2-naohthy I )PvrazinoiZ.rO.UpyridoreAblindole -1,4-dione

C 25 The same two step procedure but starting from methylamine and intermediate 18 gave, after recrystallisation from dichloromethane/methanol, the title compound as white crystals m.p.: >260’C.

Analysis for C25H21N3O2 (O.25H2O):

Calculated: C,75.08,H,5.42,N,10.51;

Found:C,75.35;H,5.42;N,10.49%.

Example 51

Cis-2.3.6.7.12.12a-hexahydro-2-butyl-6-(2-thienyl)-pyrazinof2^,,r:6.11oyridof3.4blindole -1.4-dione

AP/P/ 9 5 / 0 0 7 1?

1233CV

The same two step procedure but starting from butylamine and intermediate 20 gave, after recrystallisation from ethanol, the title compound as white crystals m.p. : 226°C.

Analysis for 022Η23Ν3θ2θ^:

Calculated. C,67.15;H,5.89;N,10.68,

Found:C,67.39;H,5.88;N,10.77%.

Example 52

Cis-2.3,6.7.12.12a-hexahydro-6-(5-bromo-2-thienyl)-2-methyl. .10 pyrazinof2', 1 ':6.11pyridoi3,4-blindole -1,4-dione

The same two step procedure but starting from methylamine and q intermediate 24 gave, after recrystallisation from ethanol, the title compound as a cream powder m.p. : 258°C.

Analysis for C-jgHi6BrN3O2S;

Calculated: C,53.03;H,3.75;N,9.76;

Found:C, 53.01; H.3.78; N, 9.69%.

Example 53

Cis-2,3.6,7.12,12a-hexahvdro-6-(4-bromo-2-thienyl)-2-methyl20 pvrazinoi2',1':6,1lpvrido[3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 26 gave, after recrystallisation from ethanol, the title compound as Qj white crystals mp.: 292°C.

Analysis for CigH-igBrNgC^S (0.25H2O):

C 25 Calculated: C,52.48;H,3.82;N,9.66;

FoundO,52.46;H,3.81 ;N,9.60%.

Example 54

Cis-2.3.6.7,12.12a-hexahydro-6-(5-bromo-2-thienyl)-2-cvclopropvlmethvl30 pyrazinof2’, 1':6.11ovridof3,4-b1indole-1.4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 24 gave, after recrystallisation from ethanol, the title compound as white crystals m.p.: 190°C.

Analysis for θ22^Η2θΒ^Γ^3θ2θ

Calculated: C,56.18;H,4.29;N,8.93; -—

AP/P/ 9 5 / 0 0 7 12

AP. Ο Ο 5 5 6

Found:C,55.92;H,4.28;N,8.74%.

Example 55

Cis-2,3,6,7,12.12a-hexahvdro-6-(5-bromo-2-thienvl)-2-cvclopentvl5 pvrazinof2'· 1 ':6.11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from cyclopentylamine and intermediate 24 gave, after recrystallisation from ethanol, the title compound as white crystals m.p.: 252’C.

Analysis for ¢23^28^3028:

Calculated: C,57.03;H,4.58;N,8.67;

Found:C,56.87;H,4.66;N,8.68%.

c

Example 56

Cis-2,3,6,7.12,12a-hexahvdro-2-methvl-6-(5-methyl-2-thienyl)15 pyrazinofZ, 1 *:6,11pvridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and the cis isomer of intermediate 66 gave, after recrystallisation from ethanol, the title compound as white crystals m.p.: 282^eC.

Analysis for C20H19N3O2S (O.25H2O):

Calculated: C,64.93.H,5.31 ;N,11.36;

Found:C,64.84;H,5.28;N, 10.81%.

Qj Example 57

Cis-2,3,6,7,12,12a-hexahydro-2-methvl-6-(3-thienvDC 25 pvrazinof2^,,r:6,1lpyrido[3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 22 gave, after recrystallisation from acetone, the title compound as white crystals m.p. : 290-295’C.

Analysis for C-19H-17N3O2S.

Calculated: C,64.94;H,4.88;N, 11.96;

Found: C, 64.81 ; H.4.95 ; N,11.68%.

Example 58

Cis-2,3,6,7,12.12a-hexahvdro-2-butyl-6-(3-thienyl)-pvrazinof2^, T:6,11pyridof3,435 blindole -1,4-dione

1233CV

The same two step procedure but starting from butylamine and intermediate 22 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 236-239’C.

Analysis for C22H23N3O2S:

Calculated: C.67.15;H,5.89;N,10.68;S,8.15;

Found:C,67.42;H₁5.76;N, 10.57;S,8.01 %.

Example 59

Cis-2,3.6,7,12.12a-hexahvdro-2-methvl-6-(3-furvn-pvrazinof2',r:6.npvridor3,410 blindole -1,4-dione

The same two step procedure but starting from methylamine and the cis isomer ζ of intermediate 28 gave, after recrystallisation from ether, the title compound as a white solid m.p. : 250*C.

Analysis for C19H-17N3O3 (O.5H2O).

Calculated: C,66.27;H,5.27;N, 12.20;

Found:C,66.33;H,5.48;N,12.02%.

Example 60

Cis-2,3,6.7,12,12a-hexahydro-2-methyl-6-(5-methvl-2-furvl)20 pyrazinof2',r:6,npyridoi3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 29 gave, after recrystallisation from ethanol, the title compound as c a cream powder m.p.: 303’C.

Analysis for C20H19N3O3 (0.25H20):

C 25 Calculated: C,67.88;H,5.55;N,11.87;

Found:C,67.90;H,5.50;N,11.98%.

Example 61

Cis-2.3.6.7.12,12a-hexahvdro-2-methvl-6-(4-methylphenvO30 pvrazino[2'.r.6,11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 31 gave, after recrystallisation from ethanol, the title compound as white crystals m.p. :>260°C.

Analysis for C22H21N3O2 (0.25 H2O);

Calculated. C,72.61 ;H,5.95;N,11.55,-------AP/P/ 95/00712

AP.00556

Found:C,72.73;H,5.96;N, 11.59%.

Example 62

Cis-2,3,6,7,12,12a-hexahvdro-2-isopropyl-6-(4-methvlphenyl)5 pvrazinofZ.rO.llpvridofSAblindole -1,4-dione

The same two step procedure but starting from isopropylamine and intermediate 31 gave, after recrystallisation from the title compound as white crystals m.p. : 170^eC.

Analysis for C24H25N3O2 (O.5H2O): _r 10 Calculated: C,72.70;H,6.61 ;N,10.60;

Found:C,73.06;H,6.43;N,9.66%.

G

Example 63

Cis-2,3.6.7.12,12a-hexahvdro-2-butvl-6-(4-methylphenyl)15 pyrazinof2', 1':6,11pyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 31 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 194*C.

Analysis for C25H27N3O2 (O.5H2O):

Calculated: C,73.15;H,6.87;N,10.24;

Found:C,73.01 ;H,6.84.N,10.26%.

Example 64

C ' 25 Cis-2,3,6,7.12,12a-hexahvdro-2-cvclopropylmethvl-6-(4-methvlphenvl)ρνΓ3ζϊηοί2'· 1 ’:6,11pyridof3.4-blindole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 31 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p.: 194°C.

Analysis for C25H25N3O2 (1.1H2O):

Calculated: C,71.61;H,6.54;N,10.02;

Found:C,71.42.H,6.07;N,9.95%.

AP/P/ 9 5 / 0 0 7 12

Example 65

1233CV

Cis-2,3,6,7.12,12a-hexahvdro-2-methvl-6-(3-methvlphenvl)pyrazirK^', 1 ' 6.11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 33 gave, after recrystallisation from ethanol, the title compound as white crystals m.p.: >260°C.

Analysis for C22H2I ^Ν3θ2;

Calculated: C,73.52;H,5.89;N,11.69;

Found.C,73.60;H,5.97;N, 11.66%.

_r 10 Example 66

Cis-2,3,6,7,12,12a-hexahvdro-2-butvl-6-(4-trifluoromethvlphenvl)pvrazinof2^<, 1 ':6,11pvridof3,4-blindole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 35 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p. : 155°C. *»

Analysis for C25H24F3N3O2 (O.5H2O):

Calculated: C,64.65;H,5.43;N,9.05; 7

Found.C,64.78,Ή,5.40,N,9.01%.

Cr

Example 67

Cis-2,3,6,7,12,12a-hexahvdro-2-methvl-6-(4-trifluoromethoxyphenvnpvrazinof2^,.r:6,11pvridof3.4-b1indole -1,4-dione q The same two step procedure but starting from methylamine and the cis isomer of intermediate 65 gave, after recrystallisation from methanol, the title compound ' (£ 25 as white crystals m.p.: 174-180’C.

Analysis for C22HI8F3N3O3 (O.5H2O):

Calculated: C,60.27;H,4.37;N,9.58;

Found.C,60.24;H,4.28;N,9.50%.

Example 68

Cis-2.3.6,7,12.12a-hexahydro-2-methyl-6-(4-hvdroxvphenyl)pyrazinofZ, 1 ':6.11pyridof3.4-blindole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 39 gave, after recrystallisation from methanol, the title compound as yellow crystals m.p. :179-180°C.----—

J θ 0 7 12

AP. Ο Ο 5 5 6

Analysis for C21 H-j9^03( 1 25H2O):

Calculated: C,65.70;H,5.64;N, 10.94;

Found:C,65.46;H,5.45;N, 10.92%.

Example 69

Cis-2.3.6.7,12.12a-hexahvdro-6-(3-hvdroxv-4-methoxvohenvD-2-methvlpyrazinof2¹· 1 ':6,11pyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 40 gave, after recrystallisation from ethanol, the title compound as white crystals m.p. :320°C.

Analysis for 022^21^304(0.251120):

( Calculated: C,66.74;H,5.47;N,10.61;

Found:C,66.72;H,5.46;N, 10.53%.

Example 70

Cis-2.3,6.7.12,12a-hexahvdro-6-(4-hvdroxv-3-methoxvphenvl)-2-methvlovrazinof2^,,r:6.npvridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 41 gave, after recrystallisation from dichloromethane/ethanol, the title compound as yellow crystals m.p. :264-265°C.

Analysis for C22H21N3O4:

Calculated: C,67.51;H,5.41;N,10.74;

Found:C,67.05;H,5.41;N,10.62%.

( 25 Example 71

Cis-2,3,6.7,12,12a-hexahydro-2-butvl-6-(4-cyanophenyl)ovrazinof2', 1':6.1 lpyridof3.4-blindole -1.4-dione

The same two step procedure but starting from butylamine and intermediate 37 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p.: 246^eC.

Analysis for C25H24N4O2 (IH2O):

Calculated: C,69.75;H,6.09;N, 13.01;

Found:C,69.50;H,5.96;N,12.86%.

Example 72

AP/P/ 9 5 / 0 0 7 12

1233CV

Cis-2.3,6.7.12.12a-hexahydro-6-(4-ethylphenvl)-2-isopropylpvrazinof2^,.1^,:6.npvridof3,4-b1indole -1.4-dione

The same two step procedure but starting from isopropylamine and the cis isomer of intermediate 42 gave, after recrystallisation from n-pentane, the title compound as white crystals m.p.: 130°C.

Analysis for C25H27N3O2 (O.5H2O);

Calculated: C,73.15;H,6.87;N, 10.24;

Found;C,73.39;H,7.08;N,9.81 %.

Example 73

Cis-2,3.6,7,12,12a-hexahydro-6-(4-ethylphenvl)-2-cvclopropylmethvl£ pyrazinof2', 1 ':6,11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and the cis isomer of intermediate 42 gave, after recrystallisation from ethanol, the title compound as white crystals m.p.; 160°C.

Analysis for C26H27N3O2:

Calculated; C,75.52;H,6.58;N, 10.16;

Found:C,75.54;H,6.62;N, 10.08%.

Example 74

C is-2.3.6.7,12.12a-hexahvdro-6-( 4-isopropvlphenvl )-2-meth vlpvrazinore'.lbe.llpyridolS^-blindole -1,4-dione 0 The same two step procedure but starting from methylamine and intermediate 43 gave, after recrystallisation from ethanol, the title compound as C 25 white crystals m.p. ; 244°C.

Analysis for C24H25N3O2:

Calculated: C,74.39;H,6.50;N, 10.84;

Found:C,74.27;H,6.53;N,11.05%.

Example 75

Cis-2.3.6.7.12.12a-hexahvdro-2-butvl-6-(4-nitrophenvl)pvrazino[2'.r:6.npvrido[3.4-blindole -1.4-dione

The same two step procedure but starting from butylamine and intermediate 45 gave, after recrystallisation from methanol, the title compound as white crystals

AP;P! 9 5 / 0 0 7 1 2

AP .0 0 5 5 6

Analysis for C24H24N4O4 (O.25H2O):

Calculated: C,65.97;H,5.65;N,12.82;

Found:C,65.92;H,5.62;N,12.96%.

Example 76

Cis-2.3.6.7.12.12a-hexahydro-6-(4-dimethylaminophenyl)-2-methylpvrazinof2^l.r:6.11pvridoi3.4-blindole -1,4-dione

The same two step procedure but starting from methylamine and the cis isomer of intermediate 47 gave after recrystallisation from methanol, the title compound as white crystals m.p. : 266°C.

Analysis for C23H24N4O2:

z' Calculated: C,71.11;H,6.23;N,14.42;

Found:C, 71.19 ; H, 6.24 ; N, 14.34%.

Example 77

Cis-2.3.6.7.12,12a-hexahvdro-2-methyl-6-(3-pyridvl)pyrazinof2^,,r:6,novridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 48 gave after recrystallisation from chloroform, the title compound as white crystals m.p.: 312°C.

Analysis for C20H18N4O2:

Calculated: C,69.35;H,5.24;N,16.17;

Found:C,69.08;H,5.20;N,16.19%.

Q 25 Example 78 (6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-methyl-6-(3.4-methvlenedioxyphenyl)ρνΓ3ΖΐηοΙ2’, Γ:6.11ovridof3.4-b1indole -1.4-dione

a) To a stirred solution of intermediate 54 (0.5 g) and NaHCO3 (0.14 g) in anhydrous CHCI3 (20 mL) was added dropwise chloroacetyl chloride (0.27 mL) at 0°C. The resulting mixture was stirred for 1 hour at the same temperature and diluted with CHCI3 (20 mL). Water (10 mL) was then added dropwise with stirring to the mixture, followed by a saturated solution of NaHCO3. The organic layer was washed with water until neutrality and dried over Na2SO4. After evaporation of the solvent under reduced pressure, (6R, 12aR)-methvl 1.2.3,4-tetrahvdro-2-chloroacetvl-1 -(3,4MH/H/ 9 5 / 0 0 7 1 2

1233CV methvlenedioxyphenvl)-9H-pyridof3.4-b1indole-3-carboxvlate was obtained as an oil which was crystallised from ether to give a solid (0.38 g, m.p. : 233^eC) which was used without further purification in the next step.

b) To a stirred suspension of the chloroacetyl intermediate (0.37 g) in MeOH (20 mL) was added at room temperature a solution of methylamine (33% in EtOH) (0.4 mL) and the resulting mixture was heated at 50^eC under N2 for 16 hours. The solvent was removed under reduced pressure and the residue was dissolved in CH2CI2 (50 mL). After washing with water (3x20 mL), drying over Na2SO4 and evaporating to dryness, the residue was purified by flash chromatography eluting with CH2Cl2/MeOH (99/1) and recrystallised from 2propanol to give the title compound as white crystals (0.22 g) m.p. : 302303”C.

Analysis for C22^H19N3O4:

Calculated;C,67.86;H,4.92;N,10.79;

Found:C,67.77;H,4.92;N,10.74%.

20° [a]_D =+71.0° (C=1.00; CHCy.

The following compounds were obtained in a similar manner;

ΓΊ Example 79 (6R, 12aR)-2.3.6.7,12,12a-Hexahydro-2-isopropyl-6-(3,4ζ 25 methylenedioxvphenyl)-pyrazinof2^l. 1 ':6,11pyridef3.4-b1indole -1,4-dione

The same two step procedure but starting from isopropylamine and intermediate 54 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 290-293’C.

Analysis for C24H23N3O4:

Calculated: C,69.05;H,5.55;N, 10.07;

Found:C,69.06,H,5.49;N, 10.12%.

20° [<x]_D =+52.6° (C=1.14; CHClg).

AP/P/ 9 5 / 0 0 7 1 2

Example 80

1233CV

AP.00556 (6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-butyl-6-(3,4-methylenedioxyphenvl)pyrazinof2'· Γ:6.11pyrido[3,4-blindole -1,4-dione

The same two step procedure but starting from butylamine and intermediate 54 gave, after recrystallisation from toluene/hexane, the title compound as white crystals m.p. : 209-21 O’C.

Analysis for C25H25N3O4:

Calculated: C,69.59;H,5.84;N,9.74;

Found:C,69.70,H,5.93;N,9.74%.

20’ [a]_D =+50.2’(C=0.53; CHCI₃).

Example 81 (6R,12aR)-2,3.6,7.12,12a-Hexahvdro-2-isobutvl-6-(3.4-methvlenedioxyphenvhpvrazinof2’.r:6.npyridof3.4-blindole -1.4-dione 15 The same two step procedure but starting from isobutylamine and intermediate gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 227-228’C.

Analysis for C25H25N3O4.

Calculated: C,69.59;H,5.84;N,9.74;

Found:C,69.52;H,5.87;N,9.74%.

20’ [a]_D = +45° (C=1.04; CHCy.

Example 82 ( 25 (6R, 12aR)-2,3,6,7,12.12a-Hexahvdro-2-cyclopentyl-6-(3,4methylenedioxyphenyl)-pvrazinoί2^,, 1 ':6,11pyridof3.4-b1indole -1,4-dione The same two step procedure but starting from cyclopentylamine and intermediate 54 gave, after recrystallisation from ether, the title compound as white crystals m.p. : 237-239’C.

Analysis for C26H25N3O4:

Calculated: C,70.41 ;H,5.68;N,9.47;

Found:C,70.13.H,5.67.N,9.42%.

20’ [«]_D ^{= +3θ 6}° (C=0.98; CHClg). 4___

AP/P/ 9 5 / 0 0 7 1 2

1233CV

Example 83 (6R, 12aR)-2,3.6,7,12,12a-Hexahvdro-6-(3.4-methylenedioxyphenvl)-2cvclohexvlmethvl-pvrazinof2',r:6,npvridof3.4-b1indole -1.4-dione 5 The same two step procedure but starting from cyclohexylmethylamine and the cis isomer of intermediate 56 gave, after recrystallisation from 2-propanol the title compound as white crystals m.p.; 209°C.

Analysis for C28H29N3O4:

Calculated: C,71 32;H,6.20;N,8.91; f 10 Found:C,71.30;H,6.29;N,8.74%.

20° [a]_D = +40.0° (C=0.99; CHClg).

Example 84 (6R, 12aR)-2,3.6,7.12.12a-Hexahvdro-2-cvclopropvlmethyl-6-(4-methoxyphenvDpyrazinof2'.r:6.11pvridof3.4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 57 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 204-205°C.

Analysis for C25H₂5N303(0.5H₂0):

Calculated: C,70.74;H,6.17;N,9.90;

Found:C,70.98;H,6.09;N,9.92%.

C 20° [a]_D =+54.1°(C=1.03;CHCI₃).

(25

Example 85 (6R, 12aR)-2,3,6,7,12.12a-Hexahvdro-2-butyl-6-(4-methoxvphenvDpvrazinof2^,.r:6.11pvridof3.4-b1indole -1,4-dione

The same two step procedure but starting from buylamine and intermediate 57 gave, after recrystallisation from 2-propanol, the title compound as white crystals m.p.: 183-184°C.

Analysis for C25H27N303(0.5H20):

Calculated: C,70.40;H,6.62;N,9.85;

Found:C,70.55;H,6.64,N,9.92%. AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6

20° [a]_D =+45.4° (C=1.04; CHClg).

Example 86 (6R, 12aR)-2,3.6.7.12.12a-Hexahvdro-2-cvclopentvl-6-(4-methoxvphenvl)pyrazinore', 1 ':6,11pyridof3.4-blindole -1.4-dione

The same two step procedure but starting from cyclopentylamine and intermediate 57 gave, after recrystallisation from ether, the title compound as white crystals m.p.: 210-211 °C.

Analysis for C26H27N3O3 C Calculated: C,72.71 ;H,6.34;N,9.78,

Found:C,72.53;H,6.39;N,9.53%.

P 20° [a]_D = +29.8° (C=1.07; CHCy.

Example 87 (6R, 12aR)-2,3,6.7,12,12a-Hexahydro-6-(3-chloro-4-methoxyphenvl)-2cvclopropylmethvl-pyrazinoi2', 1': 6.1Ipyridof3.4-b1indole -1,4-dione

The same two step procedure but starting from cyclopropylmethylamine and intermediate 59 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 218-219’C.

Analysis for C25H24CIN3O3 (0.25 H2O):

q Calculated: C,66.08;H,5.43;N,9.25 ; Cl, 7.80;

Found: C, 66.11 ; H, 5.33 ; N, 9.03 ; Cl. 7.74%.

20° ^V [a]_D = +49.4° (C=1.03; CHCy.

AP/P/ 95/007 12

Example 88 (6R,12aR)-2.3.6.7.12.12a-Hexahydro-2-cvclopentyl-6-(3-chloro-430 methoxvphenvn-pvrazinof2^,.r:6,1lpyrido[3,4-b1indole -1,4-dione

The same two step procedure but starting from cyclopentylamine and intermediate 59 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 260-262°C.

Analysis for C26H26CIN3O3:

Calculated: C,67.31 ;H,5.65,0,7.64;N,9.06; ____

1233CV

Found.C,66.98,Ή,5.67;CI,8.06;N,9.04%.

20° [α]_θ =+27.6° (C=1.05; CHClg).

Example 89 (6R, 12aR)-2,3,6,7.12,12a-Hexahvdro-6-f3-chloro-4-methoxvphenvl)-2-methvlpvrazinof2', 1 ':6,11pvridof3,4-blindole -1,4-dione

The same two step procedure but starting from methylamine and 10 intermediate 59 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 283-284°C.

Analysis for C22H20CIN3O3:

f ¹ Calculated; C,64.47;H,4.92;CI,8.65;N,10.25;

Found;C,64.49;H,4.92.CI8.33.N,10.02%.

20° + [a]_D = +61.3° (C=1.00; CHCy.

Example 90 (6R,12aR)-2,3,6,7,t2.12a-Hexahydro-2-isopropvl-6-(3-chloro-4-methoxvphenvl)- 0 pyrazinof2^l, 1 ':6,11pyridof3,4-b1indole -1,4-dione **·

The same two step procedure but starting from isopropylamine and intermediate ^c gave, after recrystallisation from methanol, the title compound as white , crystals m.p.. 302-304°C.

Analysis for C24H24CIN3O3. .

_r 25 Calculated; C,65.83;H,5.52;N,9.60;

Found:C,65.83;H,5.57.N,9.73%.

20° [<x]_D = +39.8° (C=0.95; CHCy.

Example 91 (6R, 12aR)-2.3.6,7,12,12a-Hexahvdro-6-(2,3-dihvdrobenzofblfuran-5-vl)-2methvl-pvrazinof2^,, 1 ’:6,11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylamine and intermediate 61 gave, after recrystallisation from dichloromethane/methanol, the title compound as white crystals m.p. : 288-291 °C.----—1233CV

AP. Ο Ο 5 5 6

Analysis for C23H21N3O3:

Calculated: C,71.30;H,5.46;N, 10.85;

Found:C,71.27;H,5.49;N,10.96%.

20’ [a]_D = +65.6’ (C=0.4; CHCy.

Example 92 (6R, 12aR)-2,3.6,7.12,12a-Hexahvdro-6-(2,3-dihvdrobenzofblfuran-5-yl)-2(- 10 methylcyclopropvl-pvrazinof2', 1 ':6,11pyridof3,4-b1indole -1,4-dione

The same two step procedure but starting from methylcyclopropylamine and intermediate 61 gave, after recrystallisation from methanol, the title compound as white crystals m.p.: 242-244’C.

Analysis for C26H25N3O3:

Calculated: C,73.05;H,5.89;N,9.83;

Found:C,72.90;H,5.93;N,9.98%.

20’ [ot]_D = +55.4’ (C=0.99; CHClg).

Example 93 (6R.12aR)-2.3,6.7,12.12a-Hexahvdro-6-(5-indanvD-2-methvlpyrazinof2’, 1':6,11pyridof3,4-b1indole -1.4-dione φ The same two step procedure but starting from methylamine and intermediate 63 gave, after recrystallisation from methanol, the title compound ( 25 as white crystals m.p.: 262’C.

Analysis for C24H23N3O2:

Calculated: C,74.78;H,6.01;N,10.90;

Found:C,74.65;H,5.90;N, 10.67%.

20’ i«J_D = +68 θ° (C=0.98; CHClg).

Example 94 (6R,12aR)-2.3.6.7,12.12a-Hexahydro-6-(5-indanvl)-2-cvclopropvlmethylpvrazino[2'.r:6.npyrido[3.4-b1indole -1.4-dione

1233CV

The same two step procedure but starting from cyclopropylmethylamine and intermediate 63 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 176°C.

Analysis for C27H27N3O2 (O.25H2O):

Calculated: C,75.41 ; H, 6.45 ; N, 9.77;

Found.C, 75.25 ; H, 6.51 ; N, 9.75%.

20° [a]_D = +57.9° (C=1.00; CHClg).

r ¹⁰

Example 95 (6R,12aR)-2,3.6,7.12.12a-Hexahydro-2-methyl-6-(3,4-methylenedioxyphenvl)pyrazinof2', 1 ':6,11pyridof3,4-b1indole-1.4-dione

To a stirred suspension of Intermediate 73 (12.5g) in MeOH (400ml) was added 15 at room temperature a solution of methylamine (33% in EtOH) (13.7ml) and the resulting mixture was heated at 50°C under N2 for 14 hours. The solvent was removed under reduced pressure and the residue was dissolved in CH2CI2 (IQAfter washing with water (3 x 500ml), drying over Na2SO4 and evaporating to dryness, the white solid obtained was recrystallised from 2-propanol to give the title compound as white needles (7.5g). mp : 298-300°C.

20°

Q [a]_D = ^{+ 71} -³° (^c = °·⁵⁵· CHClg).

Elemental analysis (C22H19N3O4) calculated: C, 67.86; H, 4.92; N, 10.79;

Q 25 found: C, 67.79, H, 4.95; N, 10.61%.

Example 96

Cis-2.3,6,7,12,12a-hexahydro-2,10-dimethyl-6-(3,4-methylenedioxyphenvl)pvrazinef2'.r : 6, npyridof3,4-b1indole-1,4-dione 30 The same two step procedure as used to prepare Example 1, but starting from methylamine and the cis isomer of Intermediate 74, gave after recrystallisation from ethanol, the title compound as white crystals m.p. : 275^eC.

Analysis for C23H21N3O4 (O.4H2O):

Calculated : C, 67.27 ; H, 5.35 ; N, 10.23;

Found : C, 67.36 ; H, 5.21 ; N, 10.31%.--------AP/P/ 9 5 / 0 0 7 1 2

AP. Ο Ο 5 5 6

Example 97 (6R, 12aR)-2,3,6.7,12,12a-Hexahvdro-2-(3,4-dimethoxybenzyl)-6-(3.4methvlenedioxyphenvl)-pvrazinof2^l.r : 6.11pvridof3.4-b1indole-1,4-dione 5 The same two step procedure as used to prepare Example 78, but starting from veratrylamine and intermediate 54 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 224-226’C.

Analysis for C30H27N3O6:

Calculated ; C.68.56 ; H.5.18 ; N.8.00;

Found : C,68.80 ; H.5.11 ; N,8.06%.

20° (a]_D = +43.9’(C = 1.02; CHCI₃₎.

Example 98

Cis-2.3.6,7,12,12a-hexahvdro-6-(4-aminophenvl)-2-butvlpyrazinore¹, T:6.11pyridof3.4-b1indole-1.4-dione

To a solution of Example 75 (1.5 g) in methanol (100 mL) was added SnCl2-H2O (3.06) and the resulting mixture was heated at reflux for 8 hours. The mixture was cooled to ambient temperature, poured into ice and was adjusted to pH5 with 1N NaOH. The methanol was evaporated off and the residue was basified to pH11 with 1N NaOH and extracted with EtOAc (2 x 150 mL). After drying over Na2SO4 and evaporation of EtOAc, the resulting yellow powder was purified by radial chromatography eluting with CH2CI2 to give the title compound as a white powder (550 mg) m.p.: 192’C.

f 25 Analysis for C24H26N4O2 (1.3 H2O):

Calculated : 0,67.68 ; H.6.77 ; N, 13.15;

Found : C,67.74 ; H, 6.68 ; N, 13.02%.

Example 99

Cis-2,3,6.7,12,12a-hexahvdro-6-(4-acetamidophenvl)-2-butvlpvrazinof2'· 1 ':6,11pyridof3,4-blindole-1.4-dione

To a solution of Example 98 (0.2 g) in THF (15 mL) was added triethylamine (76 pL) and acetyl chloride (39 pL) and the resulting solution was stirred at room temperature for 2 hours. After evaporation of THF, the resulting residue was taken up in CH2CI2 (100 mL), washed with water (2 x 50 mL) and dried over

ΛΕ.79 5 / 0 0 7 1 2

1233CV

Na2SC>4· After evaporation of CH2CI2, the resulting solid was recrystallised from Me0H/H20 to give the title compound as a cream powder (120 mg) m.p. : 246°C.

Analysis for C26H28N4O3:

Calculated : C,70.25 ; H.6.35 ; N, 12.60;

Found : C.69.85 ; H, 6.38 ; N, 12.56%.

Example 100

Cis-2.3,6,7.12,12a-hexahydro-2-butyl-6-(4-methylsulfonamidophenyl)10 ρνΓ3ζίηοί2', Γ:6,1 lpyrido[3,4-b1indole-1.4-dione

To a solution of Example 98 (0.2 g) in THF (5 mL) was added triethylamine (228 pL) and methanesulfonyl chloride (126 pL) and the solution was heated at reflux for 6 hours. After evaporation of THF, the residue was taken up in CH2CI2, washed with water and dried over Na2SC>4. After evaporation of CH2CI2, the residue was purified by radial chromatography eluting with CH2Cl2/MeOH (95/5) to give the title compound as a brown powder (30 mg) m.p.: 188°C. Analysis for C25H28N4O4S (0.75 H2O):

Calculated : C,60.77 ; H.6.02 ; N,11.34;

Found . C,60.61 ; H, 6.02 ; N, 10.82%.

Example 101 (6R, 12aR)-2.3.6.7.12,12a-Hexahvdro-6-(3.4-methvlenedioxvohenvl), pvrazinof2'· T : 6,11 pyrido f3,4-b1 indole-1.4-dione

The same two step procedure but starting from ammonia and intermediate 54 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 285-290’C.

Analysis for C21H.17N3O4:

Calculated : C, 67.19 , H, 4.56 ; N, 11.19 ;

Found ; C, 67.30 ; H, 4.66 ; N, 11.11 %.

[a]²⁰°D ^{= + 88}° (^{c =} °·⁴⁸ - Pyridine).

Example 102 (6R, 12aR)-2.3.6,7,12,12a-Hexahvdro-6-(3,4-methylenedioxyphenvl)-2-(2propvnvD-ovrazino 12'. 1': 6,11 pyrido [3,4-bl indole-1,4-dione

AP«T/95/0 07 1f ιζοουν

AP.00556

The same two step procedure but starting from propargylamine and intermediate 54 gave, after recrystallisation from acetone, the title compound as white crystals m.p.: 271 °C.

Analysis for C24H₁₉N₃O4 .

Calculated: C, 69.72 ; H, 4.63 ; N, 10.16 ;

Found: C, 69.95 ; H, 4,66 ; N, 10.06 %.

[a]²⁰°_D = + 51.7° (c = 0.49 ; CHCI₃).

Example 103 z 10 (6R, 12aR)-2.3,6.7,12,12a-Hexahydro-2-(3.4-methylendioxvbenzyl)-6-(3,4methy^enedioxvphenvΠ-pvΓazinof2^, 1': 6,11 pyrido f3,4-b1 indole-1,4-dione . The same two step procedure but starting from piperonylamine and intermediate gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 204-206’C.

Analysis for C29H₂3N₃O₆ :

Calculated : C, 68.36 ; H, 4.55 ; N, 8.25 ;

Found : C, 68.25 ; H, 4.49 ; N, 8.41.

[a]²⁰°_D = + 43° (c = 1.01 ; CHCI₃).

Example 104 (6R, 12aR)-2,3.6,7,12,12a-Hexahydro-2-(3.4-dimethoxyphenethyl)-6-(3.4methvlenedioxvphenvD-pvrazino 12', 1': 6,11 pyrido 13,4-bl indole-1.4-dione θ The same two step procedure but starting from 3,4-dimethoxyphenethylamine and intermediate 54 gave, after recrystallisation from dichloromethane/ether, the title compound as white crystals m.p. : 265-266’C.

Analysis for 0₃ιΗ₂₉Ν₃0β:

Calculated : C, 69.00 ; H, 5,42 ; N, 7.79 ;

Found : C, 68.68 ; H, 5.35 ; N, 7.78 %.

[a]²⁰’_D = + 38.3° (c = 1.12 ; CHCI₃).

Example 105 (6R, 12aR)-2.3,6.7,12.12a-Hexahydro-2-furfurvl-6-(3.4-methylenedioxyphenyl)pyrazino Γ2*, 1': 6.11 pyrido f3,4-b1 indole-1,4-dione

1233CV

The same two step procedure but starting from furfurylamine and intermediate 54 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 219°C.

Analysis for C26H21N3O5 :

Calculated : C, 68.56 ; H, 4.65 ; N, 9.23 ;

Found: C, 68.16 ; H, 4.63 ; N, 9.15 %.

[a]²⁰’_D = + 58.1° (c = 1.2 , CHCI₃) •

Example 106 (6R, 12aR)-2,3,6.7,12.12a-Hexahvdro-6-(3,4-methvlenedioxyphenyl)-2-(2thienvlmethvh-pyrazino f2‘, T : 6,11 pyrido f3,4-bl indole-1,4-dione The same two step procedure but starting from 2-thiophenemethylamine and intermediate 54 gave, after recrystallisation from methanol/water, the title compound as white crystals m.p. : 155-157’C.

Analysis for C26H21N3O4S :

Calculated : C, 66.23 ; H, 4.49 ; N, 8.91 ; S, 6.8 ;

Found : C, 66.13 ; H, 4.54 ; N, 9.12 ; S, 6.78 %.

[_a]20*Q = + 70.4” (c = 1.03 ; CHCI3).

Example 107 (6R, 12aR)-2.3.6.7,12,12a-Hexahydro-6-(4-methoxvphenyl)-2-methyl-pyrazino [2¹. 1': 6,11 pyrido f3,4-bl indole-1,4-dione θ The same two step procedure but starting from methylamine and intermediate gave, after recrystallisation from methanol, the title compound as white ς 25 crystals m.p. . 285-288°C.

Analysis for C₂₂H2iN₃O3 :

Calculated : C, 70.38 ; H, 5.64 ; N, 11.19 ;

Found : C, 70.31 ; H, 5.69 ; N, 11.29 %.

[<x]²⁰’_D = + 59° (c = 1.19 ; CHCI3).

Example 108 (6R, 12aR)-2.3.6.7,12,12a-Hexahvdro-2-ethyl-6-(4-methoxyphenvB-pvrazino Γ2’,

1': 6.Π pyrido [3,4-bl indole-1.4-dione

AP/P/ 9 5 / 0 0 7 1?

AP . Ο Ο 5 5 6

The same two step procedure but starting from ethylamine and intermediate 57 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 277’C.

Analysis for C23H23N3O3:

Calculated: C, 70.93; H, 5.95 ; N, 10.79;

Found: C, 70.90 ; H, 5.96 ; N, 10.54 %.

[a]²⁰’_D = + 52’ (c = 1.28 ; CHCI₃).

Example 109 (6R, 12aR)-2,3,6,7,12.12a-hexahydro-6-(7-(4-methyl-3,4-dihydro-2Hbenzof1,41oxazinyl))-2-methyl-pyrazinof2^,.r : 6.1lpvridof3.4-b1 indole-1,4-dione

The same two step procedure but starting from intermediate 75 and methylamine gave, after recrystallisation from ethanol, the title compound as white crystals m.p.: 285-288’C.

Analysis for C24H24N4O3 (0.5 H₂O): ·

Calculated: C, 67.75 ; H, 5.92 ; N, 13.17 ; ·

Found: C, 68.02 ; H, 6.00 ; N, 13.18 %. · [aJ²⁰*_D = + 71.7’ (c = 1, pyridine). * c

Example 110 (6R, 12aR)-2,3,6.7,12,12a-Hexahvdro-6-(5-f N-benzvlindolinvl))-2-methvlpvrazinof2'· Γ : 6.1lpyridof3,4-b1indole-1.4-dione 0 The same two step procedure but starting from intermediate 77 and methylamine gave, after recrystallisation from dichloromethane/methanol, the title compound as white crystals m.p. : 223-225’C.

Analysis for C30H28N4O2:

Calculated : C, 75.61 ; H, 5.92 ; N, 11.76 ;

Found : C, 75.2 ; H, 5.78 ; N, 11.67 %.

[α]20·_ο = + 20.4’ (c = 0.5, CHCI3).

Example 111 (6R, 12aR)-2,3,6,7,12,12a-Hexahvdro-6-(5-indolinvl)-2-methvl-pvrazinof2^,. 1':

6,1 lpyridof3.4-blindole-1,4-dione

A solution of Example 110 (1.05 g , 2.2 mmol) in methanol (100 mL) was hydrogenated in the presence of 10 % Pd-C (100 mg) for 48 hours at room

1233CV temperature. After removal of the catalyst, the solvent was evaporated in vacuo to leave a residue which was purified by flash chromatography eluting with dichloromethane/methanol ; 96/4. The solid obtained was recrystallised from dichloromethane/methanol to give the title compound (300 mg) as white crystals

m.p.: 240°C.

Analysis for C23H22N4O2 (0.5 H₂O):

Calculated : C, 69.86 ; H, 5.86 ; N, 14.17 ;

Found : C, 70.13 ; H, 5.77 ; N, 14.06 %.

[<x]²°’_D = + 55.9» (_C = 1.18 ; pyridine).

Example 112

Cis-2.3.6.7,12.12a-hexahvdro-6-(4-ethylphenyl)-2-methyl-pyrazinof2'.T :

^! 6,11pyridof3.4-b1indole-1,4-dione

The same two step procedure but starting from methylamine and the cis isomer of intermediate 42 gave, after recrystallisation from methanol, the title compound as white crystals m.p. : 254°C.

Analysis for C23H23N3O2 (0.25 H2O):

Calculated : C, 73.09 , H, 6.27 ; N, 11.12 ;

Found; C, 73.03 ; H, 6.18 ; N, 11.36 %.

Example 113 (6R, 12aR)-2.3.6.7.12.12a-Hexahvdro-6-(4-carbomethoxvohenvl)-2-methvlpyrazinofZ.r : 6.1lpyridof3.4-blindole-1.4-dione

The same two step procedure but starting from intermediate 78 (cis isomer) and methylamine gave, after recrystallisation from methanol, the title compound as white crystals m.p. ; 308-312°C.

Analysis for C23H21N3O4 :

Calculated : C, 68.47 ; H, 5.25 ; N, 10.42 ;

Found . C, 68.76 ; H, 5.18 ; N, 10.35 %.

[a]2°*_D = + 97.7° (c = 1, pyridine).

Example 114 (5aR, 12R, 14aR)-1.2.3.5a,6,11,12,14a-Octahydro-12-(3,4methvlenedioxvphenvl)-pvrrolof1,2: 4^,.5^,1pvrazinof2^,.1' : 6,1lpyridor3.435 blindole-5-1.4-dione

AP/P/ 9 5 / 0 0 7 12

AP.00556

Ο ί 25

A solution of intermediate 80 (0.7 g, 1.2 mmol) in a mixture of methanol/THF (80/40 mL) was hydrogenated in the presence of 10 % Pd-C (75 mg) for 48 hours at 40°C. After removal of the catalyst, the solvent was evaporated in vacuo to leave a residue, which was purified by flash chromatography eluting with dichloromethane/methanol : 98/2. The white solid obtained was recrystallised from methanol to give the title compound (180 mg) as white crystals m.p.: 284-287’C.

Analysis for C24H21N3O4 :

Calculated . C, 69.39 ; H, 5.10 ; N, 10.11 ;

Found ; C, 69.47 ; H, 5.11 ; N, 9.97 %.

[α]20·_ο = + 21.7° (c = 0.64, CHCI3).

Example 115 (5aR, 12R, 14aS)-1,2.3,5.6.11,12.14a-Octahvdro-12-(3.4methvlenedioxvphenvl)-pvrrolofr^,.2^lt: 4'.5^,1pvrazinof2^,.1^l: 6,1lovridoi3.4blindole-5-1.4-dione u

A solution of intermediate 81 (0.8 g, 1.37 mmol) in methanol (40 mL) was c hydrogenated in the presence of 10 % Pd-C (100 mg) for 5 h at 45°C. After * removol of the catalyst the solvent was evaporated in vacuo to leave a residue, ^c which was purified by flash chromatography eluting with dichloromethane/methanol ; 98/2. The solid obtained was recrystallised from _ methanol to give the title compound (300 mg) as white crystals m.p. : 302- h

304°C.

Analysis for C24H21N3O4 :

Calculated : C, 69.39 ; H, 5.10 ; N, 10.11 ;

Found: C, 69.35 ; H, 5.11 ; N, 10.10 %.

[α]20·_ο = + 106.8° (c = 1.08, CHCI₃).

Example 116 (3R, 6R, 12aR)-2,3,6,7,12.12a-hexahydro-2,3-dimethvl-6-(3,4methvlenedioxvphenvl)-pyrazinof2^,.r : 6,11pyridof3.4-b1indole-1,4-dione

To a stirred solution of intermediate 82 (0.15 g, 0.34 mmol) in THF (15 mL) was added at room temperature a solution of methylamine (33 % in EtOH) (0.32 mL)

1233CV and the resulting solution was heated at reflux under N₂ for 24 hours. The solvent was removed under reduced pressure and the residue was dissolved in CH2CI2 (25 mL). After washing with water (2 x 20 mL), drying over Na₂SO4 and evaporating to dryness, the crude product was purified by flash chromatography eluting with dichloromethane/methanol : 99/1. The white solid obtained was recrystallised from methanol to give the title compound as white crystals (80 mg) m.p. : 219-220’C.

Analysis for C23H21N3O4;

Calculated : C, 68.47 ; H, 5.25 ; N, 10.42 ;

_r 10 Found ; C, 68.39; H, 5.21; N, 10.42%.

[_α]20·_ο = + 89.6’ (c = 1 ; CHCI₃).

Example 117 (3S, 6R, 12aR)-2.3.6.7,12.12a-hexahvdro-2,3-dimethyl-6-(3.415 methvlenedioxyphenvn-pvrazinof2^,.T : 6.11pyridof3.4-b1indole-1.4-dione

To a stirred solution of intermediate 83 (0.3 g, 0.68 mmol) in THF (30 mL) was added at room temperature a solution of methylamine (33 % in EtOH) (0.68 mL) and the resulting solution was treated at reflux under N₂ for 6 days. The solvent was removed under reduced pressure and the residue was dissolved in CH₂CI₂ (50 mL). After washing with water (2,25 mL), drying over Na₂SO4 and evaporating to dryness, the crude product was purified by flash chromatography eluting with dichloromethane/methandl : 99/1. The oily residue obtained was © crystallised from methanol to give the title compound as white crystals (40 mg)

m.p. : 307-309’C.

( 25 Analysis for C23H21N3O4 .

Calculated ; C, 68.47 ; H, 5.25 ; N, 10.42 ;

Found ; C, 68.35; H, 5.33; N, 10.42%.

(_α]20·_ο = + 65.2’ (c = 1.15 ; CHCI₃).

Example 118 (6R, 12aR)-2,3,6,7,12.12a-Hexahvdro-6-(3.4-dihvdroxvphenyl)-2-methvlpvrazinof2', Γ : 6, npyridoi3,4-b1indole-1,4-dione

A solution of intermediate 86 (0.75 g ; 1.34 mmol) in a mixture of ethanol/THF (70/30 mL) was hydrogenated in the presence of 10 % Pd-C (75 mg) for 24 h at room temperature. After removal of the catalyst, the solvent was evaporated in ι V ........AP. 0 0 5 5 6 vacuo to leave a white solid which was recrystallisated from methanol to give the title compound (0.35 g) as white crystals m.p.: 224-226°C.

Analysis for C21H19N3O4 ;

Calculated : C, 66.83 ; H, 5.07 ; N, 11.13 ;

Found : C, 66.58 ; H, 5.01 ; N, 11.04 %.

[a]²⁰°_D = + 58.4° (c = 1.04 ; pyridine).

Example 119 (6R, 12aR)-2,3,6.7,12.12a-Hexahvdro-2-methvl-6-(5-(2.,. 10 methvlisoindolinvl))pyrazinof2', 1¹: 6,11pvridof3,4-b1indole-1,4-dione

The same two steps procedure but starting from intermediate 87 and f methylamine gave a crude oil which was purified by flash chromatography eluting with dichloromethane/methanol/triethylamine ; 92/8/0.1 %. The solid obtained was recrystallized from isopropanol/propyl ether/water to give the title compound (20 mg) as off-white crystals m.p.: 236°C.

Analysis for C24H24N4O2 (2.68 H₂O)

Calculated : C, 64.23 ; H, 6.59 ; N, 12.48 ;

Found : C, 64.21 ; H, 6.43 ; N, 12.02 %.

[a]²⁰°_D = + 61.1 ° (c = 0.5 ; CH₃OH).

Example 120

Compounds of formula (I) have been included in pharmacy formulations and θ details of such formulations are given below, f 25 TABLETS FOR ORAL ADMINISTRATION

A. Direct Compression

AP/P/9 5 / 0 0 7 1?-

1.	mg/tablet
Active ingredient	50.0
Crospovidone USNF	8.0
Magnesium Stearate Ph Eur	1.0
Anhydrous Lactose	141.0

1233CV

The active ingredient was sieved and blended with the excipients. The resultant mix was compressed into tablets.

2.	mg/tablet
Active ingredient	50.0
Colloidal Silicon Dioxide	0.5
Crospovidone	8.0
Sodium Lauryl Sulphate	1.0
Magnesium Stearate Ph Eur	1.0
Microcrystalline Cellulose USNF	139.5

B. WET GRANULATION

AP/P/ 95/00712

1.	mg/tablet
Active ingredient	50.0
Polyvinyl pyrollidone	150.0
Polyethylene glycol	50.0
Polysorbate 80	10.0
Magnesium Stearate Ph Eur	2.5
Croscarmellose Sodium	25.0
Colloidal Silicon Dioxide	2.5
Microcrystalline Cellulose USNF	210.0

The polyvinyl pyrollidone, polyethylene glycol and polysorbate 80 were dissolved in water. The resultant solution was used to granulate the active ingredient. After drying the granules were screened, then extruded at elevated temperatures and pressures. The extrudate was milled and/or screened then was blended with the microcrystalline cellulose, croscarmellose sodium, colloidal silicon dioxide and magnesium stearate. The resultant mix was compressed into tablets. —

Ί ? « <f \/

I A- V

AP.00556

2.	mg/tablet
Active ingredient	50.0
Polysorbate 80	3.0
Lactose Ph Eur	178.0
Starch BP	45.0
Pregelatinised Maize Starch BP	22.5
Magnesium Stearate BP	1.5

The active ingredient was sieved and blended with the lactose, starch and pregelatinised maize starch. The polysorbate 80 was dissolved in purified water. Suitable volumes of the polysorbate 80 solution were added and the powders were granulated. After drying, the granules were screened and blended with the magnesium stearate. The granules were then compressed into tablets.

Tablets of other strengths may be prepared by altering the ratio of active ingredient to the other excipients.

FILM COATED TABLETS

AP/P/ 95/00712

The aforementioned tablet formulations were film coated.

Coating Suspension	% w/w
Opadry white!	13.2
Purified water Ph Eur	to 100.0*

* The water did not appear in the final product. The maximum theoretical weight of solids applied during coating was 20mg/tablet.

f Opadry white is a proprietary material obtainable from Colorcon Limited, UK which contains hydroxypropyl methylcellulose, titanium dioxide and triacetin.

The tablets were film coated using the coating suspension in conventional film coating equipment. 25

1233CV

CAPSULES

1.	mg/capsule
Active ingredient	50.0
Lactose	148.5
Polyvinyl pyrollidone	100.0
Magnesium Stearate	1.5
The active ingredient was sieved and blended with the excipients. The mix was filled into size No. 1 hard gelatin capsules using suitable equipment.
2.	mg/capsule
Active ingredient	50.0
Microcrystalline Cellulose	233.5
Sodium Lauryl Sulphate	3.0
Crospovidone	12.0
Magnesium Stearate	1.5

The active ingredient was sieved and blended with the excipients. The mix was filled into size No. 1 hard gelatin capsules using suitable equipment.

Other doses may be prepared by altering the ratio of active ingredient to excipient, the fill weight and if necessary changing the capsule size.

ΜΊΡ! 95/00712

3.	mg/capsule
Active ingredient	50.0
Labrafil M1944CS	to 1.0 ml

The active ingredient was sieved and blended with the Labrafil. The suspension was filled into soft gelatin capsules using appropriate equipment.

Example 121

Inhibitory effect on cGMP-PDE cGMP-PDE activity of compounds of the present invention was measured using a one-step assay adapted from Wells at al. (Wells, J. N., Baird, C. E., Wu, Y. J.

Ί V <<f \/

AP.00556 and Hardman, J. G., Biochim. Biophys, Acta 384, 430 (1975)). The reaction medium contained 50mM Tris-HCI,pH 7.5, 5mM Mg-acetate, 250μg/ml 5Nucleotidase, 1mM EGTA and 0.15μΜ e-IH’j-cGMP. The enzyme used was a human recombinant PDE V (ICOS, Seattle USA).

Compounds of the invention were dissolved in DMSO finally present at 2% in the assay. The incubation time was 30 minutes during which the total substrate conversion did not exceed 30%.

_z-10 The IC» values for the compounds examined were determined from concentration-response curves using typically concentrations ranging from 10nM to 10μΜ. Tests against other PDE enzymes using standard methodology also showed that compounds of the invention are highly selective for the cGMP specific PDE enzyme.

-cGMP level measurements

Rat aortic smooth muscle cells (RSMC) prepared according to Chamley et al. in Cell Tissue Res. 177, 503 - 522 (1977) were used between the 10th and 25th passage at confluence in 24-well culture dishes. Culture media was aspirated 20 and replaced with PBS (0.5ml) containing the compound tested at the appropriate concentration. After 30 minutes at 37°C, particulates guanylate cyclase was stimulated by addition of ANF (100nM) for 10 minutes. At the end θ of incubation, the medium was withdrawn and two extractions were performed by addition of 65% ethanol (0.25ml). The two ethanolic extracts were pooled ζ 25 and evaporated until dryness, using a Speed-vac system. c-GMP was measured after acetylation by scintillation proximity immunoassay (AMERSHAM).

The compounds according to the present invention were typically found to 30 exhibit an IC50 value of less than 500nM, and an EC*» value of less than 5. In vitro test data for representative compounds of the invention is given in following

Table 1:---------

1233CV

Table 1

Example No.	IC50 nM	EC50 μΜ
12	10	0.15
36	<10	0.5
52	20	0.8
63	30	0.35
79	<10	0.15
82	20	0.5
84	10	0.4
89	10	<0.1
95	2	0.2
101	10	0.3
115	<10	0.4

Example 122

-Antihypertensive activity in rats

The hypotensive effects of compounds according to the invention as identified in table 2 were studied in conscious spontaneously hypertensive rats (SHR). The compounds were administered orally at a dose of 5mg/kg in a mixture of 5% DMF and 95% olive oil. Blood pressure was measured from a catheter inserted in the carotid artery and recorded for 5 hours after administration. The results are expressed as Area Under the Curve (AUC from 0 to 5 hours, mmHg.hour) of the fall in blood pressure over time.

AP/P/ 95/00712

In Vivo Results

Example No.	AUC PO (mmHg.h)
36	99
63	95
79	171
82	111
84	77
89	117

1233CV

AP. Ο Ο 5 5 6

Example No.	AUC PO (mmHg.h)
95	135
101	136

AP/P/ 9 5 / Ο Ο 7 fl

Claims

5 and salts and solvates thereof, in which;

R° represents hydrogen, halogen or C-|_6 alkyl;

R¹ represents hydrogen, Chalky!, C₂-e alkenyl, C₂-6 alkynyl, haloC-|. galkyl, C3_8cycloalkyl, C3_8cycloalkylC-|_3^alkyl, arylC-j _3alkyl or f's heteroarylC-|_3^alkyl;

10 r
2 represents an optionally substituted monocyclic aromatic ring selected from benzene, thiophene, furan and pyridine or an optionally o

( 20 2 ι

substituted bicyclic ring attached to the rest of the molecule via one of the benzene ring carbon atoms and wherein the fused ring A is a 5- or 6-membered ring which may be saturated or partially or fully unsaturated and comprises carbon atoms and optionally one or two heteroatoms selected from oxygen, sulphur and nitrogen; and R³ represents hydrogen or alkyl, or R¹ and R³ together represent a 3or 4- membered alkyl or alkenyl chain.

A compound of formula (la)

95/00 7-12 and salts and solvates thereof, in which;

R° represents hydrogen, halogen or C<|_6 alkyl;

R¹ represents hydrogen, C-i^alkyl, haloC-i^alkyl, C3„8cycloalkyl,

C3_8cycl°^alkylCi-3^alkyl, arylC-j^alkyl or heteroarylC-|_3alkyl, and 25

R² represents an optionally substituted monocyclic aromatic ring selected from benzene, thiophene, furan and pyridine or an optionally

IZOOUV

ΑΡ . 0 0 5 5 6 substituted bicyclic ring attached to the rest of the molecule via one of the benzene ring carbon atoms and wherein the fused ring A is a 5- or 6-membered ring which may be saturated or partially or fully unsaturated and comprises carbon atoms and optionally one or two heteroatoms selected from oxygen, sulphur and nitrogen.
3. A compound according to Claim 1 or 2, wherein R° represents hydrogen.
4. A compound according to any of Claims 1 to 3, wherein R¹ represents hydrogen, CMalkyl, haloCj galkyl, C3^cycloalkyl,

Cs^cycloalkylmethyl, pyridylC·) galkyl, furylC-)galkyl or optionally substituted benzyl.
5. A compound according to any of Claims 1 to 3, wherein R¹ and R³together represent a 3-membered alkyl chain.
6. A compound according to any of Claims 1 to 4, wherein R³ represents hydrogen.
7. A compound according to any of Claims 1 to 6, wherein R² represents an optionally substituted benzene, thiophene, furan, pyridine or naphthalene

AP/P/ 95/00712 ring or an optionally substituted bicyclic ring 1 or 2 and X and Y are each CH2 or 0.

where n is

A cis isomer of formula (I) represented by formula (lb) o

R'
8.

1233CV and mixtures thereof with its cis optical enantiomer, including racemic mixtures, and salts and solvates of these compounds in which R° is hydrogen or halogen and R¹, R and R³ are as defined in any preceding claim.
9. Cis-2,3,6,7,12,12a-hexahydro-2-(4-pyridylmethyl)-6-(3,4methylenedioxyphenyl)-pyrazino[2', T : 6,1 ]pyrido[3,4-b]indole-1,4-dione;

Cis-2,3,6,7,12,12a-hexahydro-6-(2,3-dihydrobenzo[b]furan-5-yl)-2methyl-pyrazino[2', 1':6,1 ]pyrido[3,4-b]indole -1,4-dione;
10 Cis-2,3,6,7,12,12a-hexahydro-6-(5-bromo-2-thienyl)-2-methylpyrazino[2', 1':6,1 ]pyrido[3,4-b]indole -1,4-dione;

, Cis-2,3,6,7,12,12a-hexahydro-2-butyl-6-(4-methylphenyl)pyrazino[2',r:6,1]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-isopropyl-6-(3,415 methylenedioxyphenyl)-pyrazino[2', 1' :6,1 ]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-cyclopentyl-6-(3,4methylenedioxyphenyl)-pyrazino[2',1':6,1]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-cyclopropyfmethyf-6-(4methoxyphenyl)-pyrazino[2', 1 ’:6,1 ]pyrido[3,4-b]indole -1,4-dione;

20 (6R,12aR)-2,3,6,7,12,12a-Hexahydro-6-(3-chloro-4-methoxyphenyl)-2methyl-pyrazino[2‘, 1':6,1 ]pyrido[3,4-b]indole -1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-2-methyl-6-(3₁4o methylenedioxyphenyl)-pyrazino(2', 1' :6,1 ]pyrido[3,4-b]indole-1,4-dione;

(6R, 12aR)-2,3,6,7,12,12a-Hexahydro-6-(3,4-methylenedioxyphenyl)25 pyrazino[2', 1': 6,1] pyrido [3,4-b] indole-1,4-dione;

(5aR, 12R, 14aS)-1,2,3,5,6,11,12,14a-Octahydro-12-(3,4methylenedioxyphenyl)-pyrrolo[1,2: ^^'ipyrazino^'J': 6,1]pyrido[3,4b]indole-5-1,4-dione;

and physiologically acceptable salts and solvates thereof.

10. (6R, 12aR)-2,3,6,7,12,12a-hexahydro-2-methyl-6-(3,4methylenedioxyphenyl)-pyrazino[2', 1':6,1 ]pyrido[3,4-b]indole -1,4-dione; and physiologically acceptable salts and solvates thereof.-1I L 0 0 I s 6 /dz'dV < 10

Ο ( 25
11. A compound according to any of Claims 1 to 10, for use in the treatment of stable, unstable and variant angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut motility.
12. Use of a compound according to any of Claims 1 to 10, for the manufacture of a medicament for the treatment of stable, unstable and variant angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut motility.
13. A method of treating stable, unstable and variant angina, hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma or diseases characterised by disorders of gut motility, in a human or non-human animal body, which method comprises administering to said body a therapeutically effective amount of a compound according to any of Claims 1 to 10.
14. A pharmaceutical composition comprising a compound of the according to any of Claims 1 to 10, together with a pharmaceutically acceptable diluent or carrier therefor.
15. A process of preparing a pharmaceutical composition comprising a compound according to any of Claims 1 to 10, which process comprises mixing said compound together with a pharmaceutically acceptable diluent or carrier therefor. -——-—

AP/P/ 95/00712

1233CV
16. A process of preparing a compound of formula (I), which process comprises:

a process (A) for preparing a compound of formula (I), wherein R³represents hydrogen which process (A) comprises treating a compound of formula (II)

OAlk

CHjHal (Π) in which Aik represents Ci_6alkyl and Hal is a halogen atom, with a primary amine R¹ NH2; or a process (B) for preparing a compound of formula (I), wherein R’ and R³together represent a 3- or 4-membered alkyl or alkenyl chain, which process (B) comprises cyclisation of a compound of formula (VIII)

AP/P/9 5 /OO 7 12 wherein Aik represents Ci^alkyl and R¹ and R³ together represent a 3- or

4-membered chain both as defined above; or a process (C) for preparing a compound of formula (I) wherein R³represents Ci.₃alkyl, which process (C) comprises cyclisation of a compound of formula (X) ---—10

1233CV

AP.00556 wherein Aik represents C^alkyl and R⁵ represents C₂.salkyl, substituted at Ci by a halogen atom; or process (A), (B) or (C) as hereinbefore described followed by

i) an interconversion step; and/or either ii) salt formation; or iii) solvate formation. /