AP517A

AP517A - Combination of atovaquone and proguanil and its use in treatment of protozoal infections.

Info

Publication number: AP517A
Application number: APAP/P/1993/000592A
Authority: AP
Inventors: Winston Edward Gutteridge; David Brian Ashton Hutchinson; Victoria Susan Latter; Mary Pudney
Original assignee: The Wellcome Foundation Ltd
Priority date: 1992-11-26
Filing date: 1993-11-25
Publication date: 1996-08-23
Also published as: SK57095A3; CN1091632A; PT670719E; WO1994012164A1; UA45948C2; JP3768231B2; TW253835B; IL107755A; AU685408B2; US5998449A; GR3033745T3; CY2193B1; PL309222A1; IL107755A0; CA2150234E; DE69328307D1; CA2150234C; NO313225B1; PE55694A1; NZ257901A

Abstract

The invention relates to combinations of

Description

INVENTORS CONTINUED

2. DAVID BRIAN ASHTON HUTCHINSON Langley Court Beckenham Kent ENGLAND

3. VICTORIA SUSAN LAKER Langley Court Beckenham Kent ENGLAND

4. MARY PUDNEY Langley Court Beckenham Kent ENGLAND

PA1405 c

c.

AP.00517

MEDICAMENTS

The present invention relates to synergistic combinations of 2-[4-(4-chlorophenyl) cyclohexyl]-3-hydroxy-l,4-naphthoquinone (atovaquone) and proguanil which have anti-parasitic activity. More particularly, the invention is concerned with pharmaceutical compositions containing said combinations, their use in the treatment of protozoal parasitic infections such as malaria and toxoplasmosis and their use in the treatment of infections caused by Pneumocystis carinii.

The compound 2-[4-(4-chlorophenyl)cyclohexyl] -3-hydroxy-1,4-naphthoquinone (atovaquone) has previously been disclosed, for example in European Patent No. 123,238 which relates to 2-substituted-3 -hydroxy-1,4-naphthoquinones of formula (I)

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2 wherein either R is hydrogen and R is selected from C alkoxy, aralkoxy.C alkyl-C alkoxy, phenyl substituted by one or^two groups selected from halogen and

C alkyl, halogen and perhalo-C alkyl or R and R are both C alkyl or phenyl, 1-6 . 1-6 1-6 and n is zero or 1, and physiologically acceptable salts thereof. The compounds are said to have antiprotozoal activity. Specifically, compounds of formula (I) wherein n is zero are said to be active against the human malaria parasite Plasmodium falciparum and also against Eimeria species such as E.tenella and E.acervulina. which are causative organisms of coccidiosis and compounds of formula (I) where n is 1 are said to be active against protozoa of the genus Theileria. in particular Tannulata or T.parva. Amongst the compounds specifically named and^ exemplified is the compound of formula (I) wherein n is zero, R is hydrogen and R is 4-chlorophenyl, i.e. atovaquone.

<o

LX o

Proguanil is a well-known drug for prophylaxis, but not treatment, of malaria. It is one

CMH/SQ/28 October 1993

..4

PA1405 of the safest antimalarial drugs and may be given to young children and pregnant women. However, resistance of P.falciparum to proguanil has appeared, particularly in South East Asia, and is an increasing problem.

In order to combat drug resistance, it is becoming standard practice to use combinations of more than one antimalarial, either simultaneously or sequentially. However, many such combinations are antagonistic, resulting in less effective treatment and the dosage regimens are often complicated, increasing the likelihood of patients failing to complete t the treatment. Accordingly, it was an object of the present invention to provide a combination of antimalarial drugs which was not antagonistic and which did not require f a complex dosing regimen.

It has now surprisingly been found that by combining, either concomitantly or sequentially, atovaquone, represented in this specification by formula (Π):-

and proguanil, potentiation of antiparasitic and particularly antimalarial activity is achieved. Furthermore a potentiating combination of the compound of formula (Π) and proguanil can be simply presented in a single pharmaceutical formulation.

In a first aspect, the present invention provides a method for the treatment and/or prophylaxis of a protozoal parasitic infection, e g. malaria or toxoplasmosis, or an infection caused by P.Carinii in mammals, including humans, which comprises administering an effective amount of atovaquone or a physiologically acceptable salt thereof and concomitantly or sequentially administering an effective amount of proguanil.

In a second aspect, the present invention provides atovaquone for use in the

CMHZSQ/28 October 1993

AP. Ο Ο 5 1 7

ΡΑ14Ο5 manufacture of a medicament, for administration either concomitantly or sequentially with proguanil, for treatment and/or prophylaxis of a protozoal parasitic infection, e g. malaria or toxoplasmosis or an infection caused by P.carinii, in mammals, including humans.

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Preferably the compound of formula (H) and proguanil are administered concomitantly.

Most preferably the compound of formula (Π) and proguanil are administered in a potentiating ratio.

Thus, according to a further aspect of the present invention, there is provided a combination of atovaquone, or a physiologically acceptable salt thereof, and proguanil wherein atovaquone, or its salt, and proguanil are present in a potentiating ratio.

The term 'potentiating ratio' is used herein to indicate that atovaquone and proguanil are i present in a ratio such that the antiparasitic activity of the combination is greater than . , that of either atovaquone or proguanil alone or of the additive activity that would be predicted for the combination based on the activities of the individual components.

L ·

Thus the individual components act synergistically in combination provided they are present in a potentiating ratio. e.

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A potentiating ratio, which may be successfully used to treat malaria, including ° hydroxynaphthoquinone resistant strains of malaria is in the range 1:0.1-1:100 of proguanil: atovaquone. Suitably, the potentiating ratio is in the range 1:0.2-1:10.

A particularly preferred potentiating ratio is in the range 1:1-1:3.

The present invention also provides in another aspect a method for the treatment and/or prophylaxis of malaria in mammals, including humans, which comprises administering an effective amount of a combination of atovaquone, or a physiologically acceptable salt thereof, and proguanil.

The hydroxyl group of atovaquone may form salts with appropriate bases, and physiologically acceptable salts of atovaquone include inorganic base salts such as alkali

CMH/SQ/28 October 1993

PA1405 metal (e.g. sodium and potassium) salts and alkaline earth metal (e.g. calcium salts; organic base salts e.g. phenylethylbenzylamine, dibenzylethylenediamine, ethanolamine and diethanolamine salts; and amino acid salts e.g. lysine and arginine.

It will be appreciated that the compound of formula (H) may exist as the cis or trans isomer, that is to say that the cyclohexyl ring may be cis or trans substituted by the naphthoquinone nucleus and the chlorophenyl group. Both cis and trans isomers and mixtures thereof in any ratio may be used in accordance with the present invention. In general when the compound is in the form of a mixture of isomers the trans isomer will be present in an amount of about 50% or will be the predominant isomer but the use of mixtures in which the cis isomer predominates is also included within the scope of the invention. The specific ratio of isomers may be varied as required; typical mixtures include those in which the cis/trans isomer ratio is about 1:1,40:60 and 5:95. For use according to the present invention the trans isomer of the compound of formula (Π), or a mixture of its cis and trans isomers containing at least 95% e g. 99% of the trans isomer, is preferred.

The compound of formula (Π) may also exist in a tautomeric form in which the hydroxyl group donates its proton to one of the oxo groups and the use of such tautomeric forms is included within the scope of this invention. However, it is believed that the stable form is that shown in formula (Π).

The amount of a combination of atovaquone and proguanil required to be effective as an antiparasitic agent will, of course, vary and is ultimately at the discretion of the medical or veterinary practitioner. The factors to be considered include the route of administration and nature of the formulation, the mammal's bodyweight, age and general condition and the nature and severity of the disease to be treated. In general, a suitable effective dose for administration to man for treatment of malaria is in the range of 2.0mg to 30mg of proguanil per kilogram bodyweight per day and 0.5mg to 30mg of atovaquone per kilogram bodyweight per day, for example from 3 to 20mg/kg/day of proguanil and 1 to 20mg/kg/day of atovaquone, particularly 5 to 15mg/kg/day of proguanil and 3 to 15mg/kg/day of atovaquone cr <5

UL· rc

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AP. Ο Ο 5 1 7

A suitable effective dose for administration to man for prophylaxis of malaria is in the range of from 3 to 20mg per kilogram bodyweight per week of each of proguanil and atovaquone for example from 6mg/kg/week to lOmg/kg/week of each of proguanil and atovaquone

It should be understood that the dosages referred to above are calculated in terms of the drugs per se.

For use according to the present invention the combination of atovaquone and proguanil is preferably presented as a pharmaceutical formulation.

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Pharmaceutical formulations comprise the active ingredients (that is, the combination of atovaquone and proguanil) together with one or more pharmaceutically acceptable carriers therefor and optionally other therapeutic and/or prophylactic ingredients. The -3 carrier(s) must be acceptable in the sense of being compatible with the other ingredients —j of the formula and not deleterious to the recipient thereof.

Accordingly, the present invention provides a pharmaceutical formulation comprising a combination of atovaquone and proguanil in association with one or more ’ pharmaceutically acceptable carriers therefor. ° to ( The present invention further provides a process for the preparation of a pharmaceutical po formulation which process comprises bringing into association a combination of atovaquone and proguanil with one or more pharmaceutically acceptable carriers therefor.

The combination of atovaquone and proguanil may conveniently be presented as a pharmaceutical formulation in unit dosage form. A convenient unit dose formulation contains the active ingredients in amounts of from lOmg to 3g each, e g. 50mg to 3g each. Typical unit doses may contain for example 500mg of atovaquone and 200mg of proguanil or 500mg of atovaquone and 500mg of proguanil.

Pharmaceutical formulations include those suitable for oral, topical (including

CMH/SQ/28 October 1993

PA1405 dermal,buccal and sublingual),rectal and parenteral (including subcutaneous, intradermal, intramuscular and intravenous), administration as well as administration by naso-gastric tube. The formulation may, where appropriate, be conveniently presented in discrete dosage units and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association the active ingredients with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.

Pharmaceutical formulations suitable for oral administration wherein the carrier is a solid are most preferably presented as unit dose formulations such as boluses, capsules or tablets each containing a predetermined amount of the active ingredients. A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active compounds in a free-flowing form such as a powder or granules optionally mixed with a binder, lubricant, inert diluent, lubricating agent, surface-active agent or dispersing agent. Moulded tablets may be made by moulding an inert liquid diluent. Tablets may be optionally coated and, if uncoated, may optionally be scored. Capsules may be prepared by filling the active ingredients, either alone or in admixture with one or more accessory ingredients, into the capsule shells and then sealing them in the usual manner. Cachets are analogous to capsules wherein the active ingredients together with any accessory ingredient(s) are sealed in a rice paper envelope. The combination of the compound of formula (Π) and proguanil may also be formulated as dispersible granules, which may for example be suspended in water before administration, or sprinkled on food. The granules may be packaged e.g. in a sachet. Formulations suitable for oral administration wherein the carrier is a liquid may be presented as a solution or a suspension in an aqueous liquid or a non-aqueous liquid, or as an oil-in-water liquid emulsion.

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Formulations for oral administration include controlled release dosage forms e.g. tablets wherein the active ingredients are formulated in an appropriate release - controlling matrix, or are coated with a suitable release - controlling film. Such formulations may be particularly convenient for prophylactic use.

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AP.00517

PA1405

The active ingredients may also be formulated as a solution or suspension suitable for administration via a naso-gastric tube.

Pharmaceutical formulations suitable for rectal administration wherein the carrier is a solid are most preferably presented as unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art. The suppositories may be conveniently formed by admixture of the active combination with the softened or melted carrier(s) followed by chilling and shaping in moulds.

Pharmaceutical formulations suitable for parenteral administration include sterile solutions or suspensions of the active combination in aqueous or oleaginous vehicles. Injectible preparations may be adapted for bolus injection or continuous infusion. Such preparations are conveniently presented in unit dose or multi-dose containers which are sealed after introduction of the formulation until required for use. Alternatively, the active ingredients may be in powder form which are constituted with a suitable vehicle, such as sterile, pyrogen-free water, before use.

The combination of atovaquone and proguanil may also be formulated as a long-acting depot preparation, which may be administered by intramuscular injection or by implantation e.g. subcutaneously or intramuscularly. Depot preparations may include, for example, suitable polymeric or hydrophobic materials, or ion-exchange resins. Such long-acting formulations are particularly convenient for prophylactic use.

It should be understood that in addition to the aforementioned carrier ingredients the pharmaceutical formulations for the various routes of administration described above may include, as appropriate one or more additional carrier ingredients such as diluents, buffers, flavouring agents, binders, surface active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like, and substances included for the purpose of rendering the formulation isotonic with the blood of the intended recipient.

Compositions suitable for veterinary use include those adapted for oral, parenteral, and intrarumenal administration.

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Methods for preparing atovaquone are described in EP 123,238, and one specific method is illustrated in Example 1.

Example 1

2-rtrans-4-(4-Chlorophenyl')cyclohexyl1-3-hydroxy-1.4-naphthoquinone

a) 4-(4-Chlorophenyl)cyclohexane-1-carboxylic Acid

Acetyl chloride (30g) and finely powdered aluminium chloride (60g) were stirred together in carbon disulphide (120 ml) and then cooled to -50 C, in a o

CCWoxitol bath. Cyclohexene (30 g), previously cooled to -50 C, was added dropwise during 10 minutes while maintaining the temperature of the reaction o o mixture at below -20 C. The mixture was stirred at -50 C for a further 60 minutes and the solvent then decanted to leave a gummy orange complex. A Γ •r * little chlorobenzene was added as the material warmed to ambient temperature;

the remainder of the chlorobenzene (total 300 ml) was then added, the soo obtained solution heated at 40 C for 3 hours with stirring, poured onto a mixture of ice and concentrated hydrochloric acid and the organic layer separated, washed with 2M hydrochloric acid, 2M sodium hydroxide and water, dried over anhydrous sodium sulphate and evaporated to dryness. The product o was distilled in vacuo, the fraction boiling at 140-154 C (0.1 mm Hg) collected, -» o

diluted with an equal volume of petroleum ether (40-60), cooled to -6 C and a + continuous stream of nitrogen gas bubbled through, and the separated colourless ‘^x solid recovered.

Bromine (2.8ml) was added to a solution of sodium hydroxide (6.2g) in water o (42 ml) at 0 C. The above-obtained substituted hexahydroacetophenone (3.lg) was dissolved in dioxan (15 ml) and the cold hypobromite solution then added, o keeping the reaction mixture at below 20 C. The reaction mixture was stirred at ambient temperature for 6 hours then allowed to stand overnight. Sodium metabisulphite was added to destroy excess hypobromite, the mixture cooled and then acidified to give a colourless solid. The solid was filtered off, washed with water, dried and recrystallised from ethanol to give 4-(4-chlorophenyl)

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AP.00517

PA1405 cyclohexane-1-carboxylic acid, m.p. 254-256 C.

b) 2-r4-(4-chlorophenyDcyclohexyll-3-chloro-1.4-naphthoquinone

A mixture of 2-chloro-1,4-naphthoquinone (3.95g, 0.02 mol), 4-(4chlorophenyl)cyclohexane-l-carboxylic acid (4.9g, 0.02 mol) and powdered silver nitrate (1.05g, 0.0062 mol) was heated to reflux with vigorous stirring in ml of acetonitrile. A solution of ammonium persulphate (12.0g, 0.0525 mol) in 50 ml of water was added dropwise over 1 hour. The mixture was refluxed for 3 hours then cooled in ice for 30 mins, after which it was filtered, and the residual sticky solid extracted twice with boiling chloroform to remove inorganic material. The chloroform was removed by evaporation to leave a yellow-brown solid (ca 2.7g). This was dissolved in 40 ml of boiling acetonitrile; a little insoluble material was removed by filtration. On cooling, the o * title compound separated as yellow crystals, (550 mg) m.p. 172-175 C.

NMR, dH(5 -DMSO) 8.05 (2H, mult., β-naphth), 7.85(2H, mult., a-naphth),

7.30 (4H, s.JPhH), 3.30 (1H, br.t., CH), 2.67 (1H, br.t., CH), 1.2-2.4 (8H, mult., 4xCH ).

c) 2-(4-(4-chlorophenvl)cyclohexyll-3-hydroxy-l.4-naphthoquinone

The product of stage (b) was suspended in 10 ml of boiling methanol and O.55g of potassium hydroxide in 5.5 ml of water was added dropwise over 15 mins.

The mixture was refluxed until a dark red solution formed, (after ca. 6 hrs) when ml of concentrated hydrochloric acid was cautiously added dropwise. The mixture was cooled and filtered, and the solid residue washed thoroughly with water. The water washings were re-acidified and filtered. The combined solid o residues (500 mg) mp 200-209 , were recrystallised from acetonitrile to give the o title product as the trans-isomer (300 mg) m.p. 216-219 C.

o

CT to pc

Example 2

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PA14O5

The following examples illustrate conventional pharmaceutical formulations which may be employed in accordance with the present invention:-
Film-coated tablet Core:
Compound of Example 1	500 mg
Proguanil hydrochloride	200 mg
Microcrystalline cellulose (Avicel PHI01)	130 mg
Hydroxypropyl cellulose, Lo-sub, (LHPC,LH11)	99 mg
Sodium starch glycollate (Explotab)	30 mg
Povidone K30	36 mg
Magnesium Stearate	5 mg
Compression weight	1000 mg
Coating:		is'
Polymer dispersion
(Hydroxypropylmethylcellulose and titanium		o
dioxide and polyethylene glycol 400 and		cr
colourant)	20 mg	X- ί-
Polishing:
Polyethylene glycol 8000	2 mg
Total weight	1022 mg
DisDersible film-coated tablet

Core;

Compound of Example 1 500 mg

Proguanil hydrochloride 200 mg

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AP.00517

PA1405

Microcrystalline cellulose (Avicel PH101)	100 mg
Hydroxypropyl cellulose, Lo-sub, (LHPC.LHl 1)	83 mg
Sodium starch glycollate (Explotab)	40 mg
Povidone K30	20 mg
Magnesium stearate	5 mg
Sodium docusate	1 mg
Magnesium aluminium silicate (Veegum F)	50 mg
Sodium saccharin	1 mg
Compression weight	1000 mg
Coating: Polymer dispersion (Hydroxypropylmethylcellulose and titanium dioxide and polyethylene glycol 400 and colourant)	10 mg
Polishing:
Polyethylene glycol 8000	2 mg
Total weight	1012 mg
BIOLOGICAL TEST RESULTS
Example 3
Comparison of drug interactions in combinations of compound of Examole 1 with nthpr
antimalarials.

In vitro drug sensitivity studies were carried out using the semiautomated technique of Desjardins (Desjardins et. al. Antimalarial Agents and Chemotherapy 1979; 16(6):710718). Antimalarial activity in this system is assessed by inhibition of radiolabelled

CMH/SQ/28 October 1993

PA1405 hypoxanthine incorporation into parasites by graded concentrations of drugs.

The antimalarial drugs for testing were dissolved in water, 95% ethanol, or DMSO, drugs dissolved in water were diluted 1:1 with 95% ethanol and drugs dissolved in ethanol were diluted 1:1 with water. Drug solutions were then diluted with culture medium containing 10% human serum to starting concentrations 20-50 times the estimated IC . The drugs tested and their solvents are listed below:

Drug	Initial Solvent	Medium
Compound of Example 1	DMSO		1640
Quinine	Ethanol	^— Water	1640
Chloroquine	Water^-	Ethanol	1640	>
Mefloquine	Ethanol	^— Water	1640	I
Primaquine	Ethanol	^— Water	1640	; Ί
Artesunate	Ethanol	“ Water	1640
PM443	DMSO		1640	‘ -4
Tetracycline	DMSO		1640	-
Norfloxacin	DMSO		1640
Ciprofloxacin	DMSO		1640	ΓΪ
Proguanil	Ethanol	^— Water	Lo-folate
Cycloguanil	Ethanol	” Water	Lo-folate
Pyrimethamine	DMSO		Lo-folate
Trimethoprim	DMSO		Lo-folate
Sulfamethoxazole	DMSO		Lo-folate
Dapsone	DMSO		Lo-folate
Clopidol	DMSO		Lo-folate
Allopurinol	Ethanol	^— Water	Lo-folate
PS-15	DMSO		Lo-folate
WR99210	DMSO		Lo-folate

In order to study drug combinations, drug solutions at starting concentrations were combined in various ratios (1:5,1:2,2:1 and 5:1). Drug solutions and combinations

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PA14O5

AP.00517 were then introduced into a 96-well microtitre plate to give duplicate rows of compound of example 1, the drug being combined and four combinations of the two drugs. Serial 1:3 dilutions of the drugs with media were made to fill the 96-well microtitre plate using a 12-channel pipetter. To evaluate drugs classified as dihydrofolic acid reductase (DHFR) inhibitors, modified culture medium was used which contained only physiologic concentrations of folic acid and PABA.

The remaining biological procedures were carried out according to the Desjardins technique except that three strains of P.falciparum were used (the multi-drug resistant W-2 clone, the drug-sensitive but mefloquine resistant D-6 clone and the C2B isolate resistant to the compound of example 1) and incubation was extended for 72 hours.

Individual IC^s were calculated using the MINSQ program from Micromath Scientific Software. Each set of paired data was fitted to the hyperbolic tangent function used by Desjardins.

The IC s were normalised by assigning values of 1 to the IC for the compound of 50 50

Example 1 and to the other drug being combined with proportional normalised values for each ratio of the two drugs being studied. An isobologram was constructed by fitting the data to the equation:

Y-l-pc./pi.+e^O-X.))) where Y. = IC for compound of Example 1 when combined with another drug

X. = IC for another drug when combined with compound of ί 50

Example 1

I = interaction parameter indicating degree of reversal

Values of I were calculated for each combination, synergistic combination, negative values indicated additive interaction.

Positive values of I indicated a antagonism and 1 = 0 indicated

The results are shown in

Table

1.

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TABLE 1

Drug combined with compound of	I
Example 1	W-2	D-6	C2B

Quinine	-1.36
Chloroquine	-1.84	-1.40
Mefloquine	-1.19
Tetracycline	1.27	1.11	0.02, -0.08	>
Primaquine	-0.79
Artesunic acid		-0.18		. i
PM443		-1.28		o
Norfloxacin	1.02
Ciprofloxacin	-1.22			-
Pyrimethamine	0.36		-0.48
Trimethoprim	1.27		0.58	- r<
Proguanil	2.43, 2.88	2.56	2.56	.c.
Cycloguanil	2.21	1.66	0.13, -0.73
Allopurinol	1.14	0.43
PS-15	1.77, 0.65, 1.97	-0.74
WR99210	0.02
Sulfamethoxazole	2.75
Dapsone	-0.39
Clopidol	2.38,2.65	0.73

The results show that combinations of 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxy-1,4naphthoquinone and proguanil exhibited most consistent potentiation compared to the other drug combinations tested, particularly against the hydroxynaphthoquinone resistent C2B strain.

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ΡΑ14Ο5

The optimum ratio of the combination with proguanil was estimated for each of the three strains of malaria parasite by determining the ratio of the of proguanil to the

IC of the compound of Formula (Π). The results are given in Table 2 below.

5o

TABLE 2

W-2

D-6

C2B ₍ Proguanil: Compound of Formula (I) 920:1 4038 1 0.2.1

2473:1 r

Example 4

Comparison of anti-Toxoplasma activities in vivo of compound of Example 1. proguanil and combinations thereof

Activities of the compounds and combinations were examined in a mouse model of T.gondii, using the increase in time to death and percent survival of the mice as measures of drug activity.

J

Groups of 10, 20 gm CB A/CA mice were infected orally by gavage with 6 cysts of the , _T, ( C56 strain of T.gondii, and drug treatment was started 3 days later and continued for 10 days. All drugs were administered orally by gavage. The following groups were » □ examined:

Controls

Atovaquone @ 10 mg/kg Atovaquone @25 mg/kg Proguanil @ 25 mg/kg

Atovaquone @ 10 mg/kg + Proguanil @ 25 mg/kg Atovaquone @ 25 mg/kg + Proguanil @ 25 mg/kg

All animals were examined twice daily for 30 days and all deaths recorded.

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PA 1405

The results are shown in Tables 3 and 4:

j Proguanil (mg/kg) (

TABLE 3 Mean Time to Death (days)
	0	Atovaquone 10 mg/kg	25 mg/kg
0	140	20.1	24.1
25	9.0	18.4	29.3

TABLE 4 % Survival
	0	Atovaquone 10 mg/kg	25 mg/kg
0	10.0	10.1	10.0
25	0.0	0.0	80.0

Proguanil (mg/kg) «Ό /

V

The mean time to death of the control mice was 14 days, with only one animal surviving [10%]. (This animal may not have become infected with the low inocculum used).

Atovaquone alone increased this to 20.1 days at 10 mg/kg and 24.1 days at 25 mg/kg, in both cases with a single survivor [10%]. Proguanil, despite its excellent safety record in man, is toxic to mice. At 25 mg/kg, proguanil exhibits signs of toxicity, reducing the mean time to death to 9 days, with no survivors. The combination of 10 mg/kg atovaquone with 25 mg/mg proguanil gave a mean time to death of 18.4 days. The combination of 25 mg/kg atovaquone and 25 mg/kg proguanil gave an increased time to death in spite of the toxicity of proguanil and 80% of the mice survived compared to the minimal survival of 10% mice given atovaquone alone.

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AP.00517

Example 5

Comparison of anti-Toxoplasma activities in vitro of compound of Example 1.

proguanil and combinations thereof

In vitro dmg sensitivity studies were carried out using a semi-automated technique based on that used for malaria (Desjardins et al, Antimicrobial Agents and Chemotherapy 1979 16 (6) 710-718), but utilising the selective incorporation of ³[H]uracil by T.gondii. Antitoxoplasma activity in this system is assessed by inhibition of uptake of radiolabelled uracil into parasites by graded concentrations of drugs.

The drugs were dissolved in DMSO and dilutions prepared using culture medium containing 3% foetal calf serum. To study drug combinations, drug solutions at starting concentrations were combined in various ratios 1:1, 1:3, 3:1. Serial 1:2 dilutions of the drug solutions and combinations were prepared and used in duplicate wells of a 96 well plate previously seeded with HeLa cells and RH strain T.gondii. Drugs were added two hours after the parasite and the plates incubated at 37°C for 24 hours when the 3[H]uracil was added and incubation continued for a further 8 hours. The assay was completed by removing the supernatant fluid, disrupting the T.gondii containing cells in SDS, and precipitating the labelled proteins with TCA onto filter mats. The incorporation of label was measured on a Beta plate scintillation counter. Percent inhibition of uracil incorporation was calculated for the compounds and combinations and IC5QS calculated using the GS1 programme. IC50S were normalised by assigning values of 1 to IC50 for the compound of example 1 and proguanil with normalised values for each ratio of the two drugs being studied. An isobologram was constructed by plotting these normalised IC50S against each other. Potentiation was indicated by the values occuring below the line of the isobologram, an additive effect by values on the line and antagonism by values above the line.

Plates were set up in triplicate and all values plotted.

>

. i

-O

The results are shown in table 5.

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TABLE 5

Normalised IC50S Potentiation

Atovaquone Proguanil

0.559278	0.583893	No
0.196689	0.614094	Yes
0.729381	0.253691	Yes
0.345238	0.527273	Yes
0.130952	0.6	Yes
0.233333	0.118182	Yes	)
0.404959	0.451538	Yes	* Ί 1
0.229201	0.768462	Yes	1
0.244904	0.091538	Yes

The results show that combinations of atovaquone and proguanil exhibit potentiation in vitro against T.gondii. ³ >

π

Example 6

0

Comparison of anti-Pneumocystis activities in vivo of compound of Example 1, proguanil and combinations thereof

Activities of the compounds and combinations were examined in a scid mouse model of

Pneumocystis pneumonia.

The level of infection of mice in each group was measured using standard lung impression smears and immunofluorescence tests. A score was assigned to each mouse where 0 = no infection and +4 = very heavy infection. The results are shown in table 6.

CMH/SQ/28 October 1993

AP. Ο Ο 5 1 7

ΡΑ14Ο5

TABLE 6

Treatment SCORE No. Mean Score % of

Infected/ + SE Control +1 +3 +4 +4 No.

Examined

Control untreated	0	0	0	3	7	10/10	3.70 + 0.14	100
Atovaquone 50 mg/kg p.o. daily	0	0	5	5	0	10/10	2.50 + 0.16	68
Atovaquone 25 mg/kg p.o. daily	0	0	1	7	2	10/10	3.10 + 0.17	84
Proguanil 25 mg/kg p.o. daily	0	0	0	2	8	10/10	3.80 + 0.13	85
Atovaquone & Proguanil 50 + 25 mg/kg p.o. daily	5	4	1	0	0	5/10	0.50 + 0.21	14
Atovaquone & Proguanil 25 + 25 mg/kg p.o. daily	1	0	4	4	0	8/9	2.22 + 0.31	60

AP/F/ 9 3 / 0 0 5 9 2

When dosed alone, atovaquone gave reductions in the infection score. Proguanil alone at 25 mg/kg/day was ineffective in prophylaxis of PCP in the scid mouse. Proguanil in combination with atovaquone showed synergy.

Claims

1. A method for the treatment and/or prophylaxis of a protozoal parasitic infection or an infection caused by P.carinii in mammals which comprises administering 2-[4(4-chlorophenyl)cyclohexyi]-3-hydroxy-1,4-naphthoquinone and proguanil in a potentiating ratio.

2. A method according to claim 1 wherein the protozoal parasitic infection is malaria.

3. A method according to claim 1 wherein the protozoal parasitic infection is toxoplasmosis.

4. A method according to claim 1 for the treatment and/or prophylaxis of an infection caused by P.carinii.

5. A method according to any of claims 1 to 4 wherein the administering 2-[4-(4chlorophenyl)cyclohexy(]-3-hydroxy-1,4-naphthoquinone is in the form of the trans isomer or a mixture of cis and trans isomers in which the trans isomer predominates.

6. A combination of 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxy-1,4-naphthoquinone for the manufacture of a medicament for administration either concomitantly or sequentially with proguanil, for treatment and/or prophylaxis of toxoplasmosis or an infection caused by P.carinii in mammals.

7. A combination of 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxy-1,4-naphthoquinone and proguanil wherein the 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxy-1,4naphthoquinone and proguanil are present in a potentiating ratio.

8. A combination according to claim 7 wherein the ratio of proguanil;2-[4-(4chlorophenyl)cyclohexyl]-3-hydroxy-1,4-naphthoquinone is in the range of 1:1 to 1:3.

PA1405/AP

AP . 0 0 5 1 7

9. A combination according to claim 7 or 8 wherein the 2-(4-(4chlorophenyl)cyclohexyl]-3-hydroxy-1,4-naphthoquinone is in the form of the trans isomer or a mixture of the cjs and trans isomers in which the trans isomer predominates.

10. A combination according to any of claims 7 to 9 for use in the treatment and/or prophylaxis of a protozoal parasitic infection or an infection caused by P.carinii in a mammal.

10

11. Use of a combination according to any of claims 7 to 9 for the manufacture of a medicament for the treatment and/or prophylaxis of a protozoal parasitic infection or an infection caused by P.carinii in a mammal.

12. A method for the treatment and/or prophylaxis of a protozoal parasitic infection or

15 an infection caused by P.carinii in mammals which comprises administration of an effective amount of a combination according to any of claims 7 to 9.

13. A pharmaceutical formulation comprising a combination according to any of claims 7 to 9 in association with one or more pharmaceutically acceptable carriers

20 therefor.

14. A unit dose formulation comprising 50mg to 3g each of 2-(4-(4chlorophenyl)cyclohexyl]-3-hydroxy-1,4-naphthoquinone and proguanil.

25 15. A unit dose formulation according to claim 14 comprising 500mg of 2-(4-(4chlorophenyl)cyclohexyi]-3-hydroxy-1,4-naphthoquinone and 200 mg of proguanil.