AP503A - Insecticidal 5-substituted-2,4-diaminopyrimidine derivatives. - Google Patents

Abstract

An insecticidal composition comprising, in admixture with an agriculturally acceptable carrier, an insecticidally effective amount of compound of the formula: whereinr is (a)hydrogen, straight or branched chain alkyl, cycloalkyl,polycycloalkyl, cyano, morpholinly, alkylsilane, arylsilane, phenyl, or substituted-phenyl-phenyl of the formula:

Description

INSECTICIDAL 5-SUBSTITUTED-2.4-DIAMINOPYRIMID1NE DERIVATIVES

This invention relates to pyrimidine compounds and compositions containing the same which are useful for controlling insects in agricultural crops. Still more particularly, this invention relates to certain 2,45 diaminopyrimidine compounds and compositions, and their use as insecticides against a variety of insects, especially larvae such as the tobacco budworm. Numerous of these diaminopyrimidine compounds employed herein, and their preparation, have been described in the literature for use in a variety of fields, but not as insecticides.

In accordance with the present invention it has been found that 5substituted-2,4-diaminopyrimidines, and agriculturally acceptable salts thereof, when present in insecticidally effective amounts, and with a suitable agricultural carrier, are useful as active ingredients in the insecticidal

5 compositions and methods of this invention. These pyrimidines may be represented by the following structure:

wherein R is (a) hydrogen, straight or branched chain alkyl, cycloalkyl, polycycloalkyl (e.g., adamantyl), cyano, morpholinyl,

5 alkylsilane, arylsilane, phenyl, or substituted-phenyl of the formula:

AP/P/ 9 3 / 0 0 5 9 5

wherein

AP, 00503

-2V is hydrogen, halogen, lower alkyl, or lower haloalkyl (e.g., -CF₃);

W is hydrogen, halogen, straight or branched chain alkyl, alkylsilane, alkylsilanylalkyl, phenyl, alkoxy, haloalkyl, pyridinylalkyl, nitro, substituted-phenylalkyl, cyano, aminocarbonyl, phenyl(hydroxyalkyl), substitutedphenoxyalkyl, bicycloalkoxy, -NHC(O)R⁴, or -NHR®, wherein R⁴ is alkyl, haloalkyl, substituted phenyl, substitutedphenylalkyl, or substituted- phenoxyalkyl; R⁵ is alkyl, cycloalkyl, substituted-phenylalkyl, substituted- phenoxyalkyl, or pyridinylalkyl, wherein the phenyl substituents are halo, alkoxy, or alkylsulfonyl;

X is hydrogen, halogen, lower alkyl, lower alkoxy, alkoxycarbonyl, alkylthio, alkylsulfonyl, lower haloalkyl, aminocarbonyl, lower haloalkylsulfonyl (e.g., -SO2CF3), lower haloalkoxy (e.g., -OCF2CHF2), cyano, lower alkylcarbonylamino, (phenyl, or substituted-phenyl lower alkyl)amino, or (lower alkyl)(phenyl or substituted-phenyl lower alkyl)amino wherein the phenyl, when substituted, is substituted with at least one, and preferably two, halogens (e.g., 3.5-CI2). lower alkoxy (e.g., 4-OCH3), lower alkyl or lower haloalkyl;

Y is hydrogen, halogen, lower alkyl, lower haloalkyl (e.g., -CF3) or lower alkoxy; and

Z is hydrogen, halogen, or lower alkyl; or (b) substituted-phenyl lower alkyl of the formula:

wherein q is 1 or 2; and,

V¹« W¹, X¹, γ1 and Z¹ are independently selected from hydrogen, lower alkyl, halogen, lower alkoxy, lower haloalkyl, lower alkylsulfonyl, cyano, or aminocarbonyl; or (c) naphthyl or substituted-naphthyl of the formula:

( /'

AP/P/ 9 3 / 0 0 5 9 5 wherein

V². W², x², Y²> and Z² are independently selected from halogen, lower alkyl, lower haloalkyl, lower alkylsulfonyl, cyano, or aminocarbonyl; or (d) thiazol-2-yl, benzothiazol-2-yl, or 5-substituted-thien-2-yl of the formula:

V³ (V)

AP . ^{Γ Γ}> 5 Ο 3

-4wherein

V³ is halogen, lower alkyl, lower haloalkyl, cyano, or aminocarbonyl;

and wherein

R1 is hydrogen, lower alkyl, amino, phenyl, or phenyl lower alkyl; R2 is hydrogen or lower alkyl; R3 is hydrogen or lower alkyl; or R2 and R3, taken together, form the group -R6-O-R6-, wherein R6 is lower alkylene;

m is 0 or 1;

n is 0 to 11; and p is 0 or 1, and agriculturally acceptable salts thereof, with the proviso that when m, n, and p are 0, R is other than hydrogen; and with the further proviso that when m and p are 1, n must be at least 1.

Of these compounds, among the more preferred ones for use in the compositions and methods of this invention are those wherein the diaminopyrimidines are of the structure (I) above, and wherein (i) m and p are 0;

R is lower alkyl, cycloalkyl, e.g., cyclohexyl; polycycloalkyl, e.g., adamantyl; naphthyl or substituted-naphthyl; phenyl or substitutedphenyl of the structure

wherein

V, W, X, Y, and Z are independently hydrogen, halo, e.g. chloro; lower alkyl, e.g. methyl; lower alkoxy, e.g. ethoxy or

0 propoxy; trihaloalkyl, e.g. trifluoromethyl; nitro, phenyl, or (substituted-phenyl lower alkyl)amino [e.g., (3,5AP . ^Γ· 5 Ο 3

-5dichlorophenylmethyl)amino or (4-methoxyphenylmethyl)amino], wherein at least one of V to Z is not hydrogen;

n is 0 to 10;

R¹ is hydrogen, lower alkyl, or amino;

R² is hydrogen, or lower alkyl, e.g. ethyl; and

R³ is hydrogen;

(ii) m is 1 and p is 0;

R is pentan-3-yl, cyclopentanyl, or phenyl;

n is 1 to 3;

R¹ is hydrogen, lower alkyl, e.g. methyl or ethyl, or amino; and R² and R³ are hydrogen; or (iii) m and p are 1;

R is substituted-phenyl of the structure:

IF! 9 3 / 0 0 5 9 5 <

wherein

W, X, Y and Z are hydrogen, and V is trihaloalkyl;

n is 3;

R¹ is lower alkyl, e.g. methyl; and

R² and R³ are hydrogen, lower alkyl, or amino.

Particularly preferred amongst the above compounds having the structure of formula (I) above which may be employed in this invention are those which correspond to certain of the numbered compounds in Table 1 below; i.e., those wherein, (iv) m, n and p are 0;

R is adamantyl;

AP. Ο Ο 5 0 3

-6R¹ is lower alkyl, e.g. methyl; and

R² and R³ are hydrogen (Compound 110);

(v) m and p are 0;

n is 2;

R is substituted-phenyl lower alkyl of the formula:

(

5 (

( 20 wherein q is 1, W¹ (compound 136) or X¹ (Compound 137) is trihaloalkyl, e.g., trifluoromethyl, and wherein the remaining substituents V¹, W¹, X¹, Y¹, and Z¹ for each compound are hydrogen or halo, preferably chloro;

R1 is lower alkyl, e.g., ethyl; and

R² and R³ are hydrogen;

(vi) m and p are 0;

n is 3 or 4;

R is naphth-1-yl (Compounds 47, 48 and 50), or substituted- phenyl of the structure:

V is lower alkyl, e.g. methyl (Compound 123) or trihaloalkyl, e.g., trifluoromethyl (Compound 120); W is phenyl (Compound 33); X is

Γ

AP. 00503

-710

Γ (

( (

( halo, preferably chloro (Compound 67) or lower alkyl, e.g., methyl (Compound 125); or V, X, and Y are halo, e.g. chloro (Compounds 34, 38,117 and 118) or lower alkyl, e.g., methyl (Compound 128); or V and X are halo, preferably chloro (Compounds 136 and 139); and the remaining substituents V, W, X, Y, and Z of each compound are hydrogen;

R1 is hydrogen (Compounds 47, 50, and 67), lower alkyl, e.g. methyl (Compounds 117,120,123,125, and 128) or ethyl, (Compounds 33, 34, 38, and 118) or amino (Compound 48);

R² is hydrogen or lower alkyl, e.g. ethyl; and

R³ is hydrogen;

(vii) m is 1;

n is 1 or 3;

p is 0;

R is pentan-3-yl (Compounds 56 and 132) or phenyl (Compound 134);

R¹ is hydrogen (Compound 56) or lower alkyl, e.g. methyl (Compounds 132 and 134); and R² and R³ are hydrogen; or (viii) m and p are 1;

n is 3;

R is substituted-phenyl of the structure

AP/P' 9 5 / 0 0 5 9 5 wherein

V is trihaloalkyl, e.g. trifluoromethyl, and the remaining substituents W, X, Y, and Z are hydrogen;

R¹ is lower alkyl, e.g. methyl; and

AP. π η 5 η 3

-8R² and R³ are hydrogen (Compound 135), and agriculturally acceptable salts thereof.

Each of these above compounds, which are preferred because of 5 their high insecticidal activity, may be used in controlling insects by applying to the locus where control is desired an insecticidal amount of these compounds admixed in a suitable agricultural carrier.

For purposes of this invention, as regards the above substituent groups, the following definitions apply:

The term alkyl includes straight or branched chained alkyl of 1 to 14 carbon atoms; preferably lower straight or branched alkyl of 1 to 6 carbon atoms; while halogen includes chlorine, bromine, and fluorine atoms. The term haloalkyl and haloalkoxy include straight or branched chain alkyl of 1 to 14

5 carbon atoms, preferably lower straight or branched alkyl of 1 to 6 carbon atoms, wherein one or more hydrogen atoms have been replaced with halogen atoms. The cycloalkyl groups include polycycloalkyl, and may be saturated or unsaturated, for example cyclohexyl, cyclohexenyl, decalinyl, or adamantyl having from about 3 to at least 10 carbon atoms, and may be

0 substituted by halogen, alkyl, substituted aryl, cyano, alkoxy or the like. The term aryl and substituted aryl include phenyl and naphthyl, preferably phenyl or substituted-phenyl. The term aryl also includes those aryl groups substituted with one or more alkyl, halo, alkoxy, cycloalkyl, aryl, haloalkyl, haloalkoxy, cyano, aminocarbonyl, nitro, dialkylamino, thioalkyl, alkylsulfonyl, or like moieties.

As aforestated, the present 2,4-diaminopyrimidines, when admixed with suitable carriers and applied to insect-infected crops such as cotton, vegetables, fruits or other crops are highly effective against an array of

0 insects, particularly those shown in the tables below.

Each of the novel compounds of these additional embodiments may be prepared in the same or similar manner as those compounds of Formula I above.

5 Synthesis of The Compounds

AP. Ο Ο 5 Ο 3

-9The compounds employed as insecticides in accordance with this invention are generally known to those skilled in the art, or may readily be prepared from these compounds by known methods. These and other methods are described in further detail in the examples below. Thus, for example, those compounds wherein m is 0 and n is 1 or more, may be prepared by one of two methods, depending on whether R1 in the 6position of the pyrimidine ring is hydrogen or one of the other defined substituents.

Those compounds wherein R¹ is hydrogen may be prepared by the 1 0 method of Smimow et al., Alkylation of 3-Ethoxy-2-lithioacrylonitrile...,

C Synthetic Communications. 16(10), (1986), pp. 1187-1193, by the metallation of 3-ethoxyacrylonitrile with n-butyllithium in tetrahydrofuran at about -110°C. The resulting anion is in turn treated with an excess of an optionally substituted alkyl halide, for example 3-(naphth-1-yl)propyl iodide,

5 yielding the corresponding 1-ethoxy-2-cyano-2-acrylonitrile. The nitrile is then condensed with guanidine hydrochloride and potassium carbonate in Ν,Ν-dimethylformamide at about 125°C, affording 5*(optionally substituted alkyl)-6-unsubstituted-2,4-diamino-pyrimidines. Examples 1, 3, and 6 (below) provide the details for this route.

The 1-ethoxy-2-cyano-2-acrylonitrile may also be condensed with 1ethylguanidine hydrochloride and potassium carbonate in

(. dimethylformamide as previously described, yielding 5-(optionally substituted alkyl)-6-unsubstituted-2-ethylamino-4-aminopyrimidine.

Example 9 provides the detail for this method of preparation of a substituted amino derivative.

Compounds wherein R¹ is other than hydrogen are generally prepared by a second method which requires the metallation of an optionally substituted alkanenitrile, for example, 5-(2,4,5trichlorophenyljpentanenitrile or 7-phenylheptanenitrile, with n- butyllithium

0 in tetrahydrofuran at about -78°C. The resulting anion is then reacted with an ethyl (optionally substituted) alkanoate, for example ethyl propanoate or ethyl 5-phenylpentanoate, yielding the appropriately substituted acyanoalkanone, for example 1-(2,4,5-trichlorophenyl)-4-cyano-5-heptanone or 1,11-diphenyl-6-cyano-5-undecanone. This alkanone may then be

5 methylated on the oxygen with diazomethane in diethyl ether, affording a 1 methoxy-1- and 2-(optionally substituted alkyl)-2-cyanoethene, for example

AP/P/ 9 3 / 0 0 5 9 5

AP. η ο 5 ο 3

-101-(2,4,5-trichlorophenyl)-4-cyano-5-methoxy-4-heptene or 1,11 -diphenyl-5methoxy-6-cyano-5-undecene. The thus- prepared ethene can then be condensed with guanidine hydrochloride and potassium carbonate in Ν,Νdimethylformamide as previously described, yielding the 5-, and 65 (optionally substituted alkyl)-2,4-diamino-pyrimidine. Examples 4, 5, 7, and 8 (below) provide the details for this method.

This second method, as described above, may also be utilized to prepare compounds wherein R¹ is hydrogen. This route requires the use of ethyl formate, rather than an ethyl (optionally substituted) alkanoate.

0 Example 2 provides the detail for this method.

The above-defined compound wherein R¹ is amino may be prepared by the treatment of malononitrile with sodium hydride in tetrahydrofuran, followed by reaction of the thus-prepared sodium salt with an optionally substituted alkyl halide, for example, 3-(naphth-1-yl)propyl iodide, yielding a 2-cyano-(optionally substituted)alkanenitrile. The 2-cyano-(optionally substituted)alkanenitrile, for example 2-cyano-5-(naphth-1-yl)pentanenitrile, may then be treated with sodium ethoxide in ethanol and reacted with guanidine hydrochloride, yielding 2,4,6-triamino-5-(optionally substituted alkyl)pyrimidines. Example 11 provides the detail for this method.

0 Alternatively, there may be prepared the compound wherein m and n are 0, and R is phenyl. This compound is prepared by the reaction of C benzyl cyanide and Ν,Ν-dimethylformamide dimethyl acetal in methanol.

The thus-prepared 1-cyano-1-phenyl-2-N,N-dimethylaminoethene is then C condensed with guanidine hydrochloride and potassium carbonate in

5 dimethylformamide, as previously described, yielding 2,4-diamino-5phenylpyrimidine (I). Example 10 provides the detail for this method.

A further, alternate method used to prepare a number of compounds wherein m is 1 and R¹ is hydrogen includes condensing 3ethoxyacrylonitrile with guanidine hydrochloride and potassium carbonate

0 in Ν,Ν-dimethylformamide, as previously described, yielding 2,4-diaminopyrimidine. The pyrimidine may, in turn, be reacted with ammonium persulfate in aqueous 5N sodium hydroxide affording 2,4-diamino-5pyrimidinyl hydrogen sulfate. The hydrogen sulfate is then treated with sulfuric acid in water yielding the corresponding 2,4-diamino-5-hydroxy3 5 pyrimidine hydrosulfate salt. The thus-prepared 5-hydroxypyrimidine can then be reacted with an (optionally substituted) alkyl halide and potassium

AP.00503 c

-11 10 c

c

(.

c

(.

carbonate in Ν,Ν-dimethylformamide, yielding 2,4-diamino-5-(optionally substituted alkoxy)pyrimidines. Example 12 provides the detail tor this method.

Other methods by which intermediates ot the above-described compounds are shown in further detail in Examples 1-9 and 11 (below).

Compounds 90-96, which include the sub-structure -(O)p- may also be prepared by methods described above, e.g., using the method of Smimow et al., previously described, by the treatment of the lithiated 3ethoxyacrylonitrile with a substituted-oxaalkyl halide, for example 3-(4propylthiophenoxy)propyl iodide, yielding the corresponding 1-ethoxy-2cyano-2-(substituted-oxaalkyl)-6-unsubstituted-2,4-diaminopyrimidines. Examples 1, 3, and 6 also show this method.

Another class of compounds, represented by Compounds 100-103, 105-109, wherein m is 1, n and p are 0, and R is substituted phenyl, may be prepared in accordance with the methods shown in Example 14. tn this method, ethyl 3-oxopentanoate is chlorinated with sulfuryl chloride, affording the corresponding ethyl 2-chloro-3-oxopentanoate. The ethyl 2chloro-3-oxopentanoate is then reacted with sodium hydride, then treated with an appropriately substituted phenol, yielding ethyl 2-(substitutedphenoxy)-3-oxopentanoate. The thus-prepared ethyl pentanoate is cyclized with guanidine carbonate in ethanol, yielding 2-amino-6-ethyl-4hydroxy-5-(substituted phenoxy)pyrimidine. The 4-hydroxyprimidine is then chlorinated with phosphorus oxychloride, providing the corresponding 2amino-4-chloro-6-ethyl-5-(substituted-phenoxy) pyrimidine. This compound in turn is treated with ammonia gas in ethanol under pressure, yielding 2,4diamino- 6-ethyl-5-(substituted-phenoxy)-pyrimidine. Compounds 72-89, wherein m, n, and p are 0, and R is:

AP/P/ 9 3 / 0 0 5 9 5 (Π) may be prepared in accordance with the methods of Example 13. In these compounds, V, Y, and Z are hydrogen, X is chloro, and W is -NHR5, or -NHC(O)R⁴; wherein R⁴ is straight or branched chain alkyl of 4 to 10 carbon

AP. 00503

-12atoms in length, lower haloalkyl, substituted-phenyl, substitutedphenylalkyl, and substituted-phenoxyalkyl; and Ft⁵ Is straight chain alkyl, cycloalkyl, substituted-phenylalkyl, and substituted-phenoxyalkyl; and the substituents on the phenyl moiety include, preferably, halo, alkoxy, and alkylsulfonyl.

In this method, commercially available 2,4-diamino-6-ethyl-5-(4chlorophenyl)pyrimidine is nitrated with 70% nitric acid and concentrated sulfuric acid, yielding the corresponding 2,4-diamino-6-ethyl-5-(4-chloro-3nitrophenyl)pyrimidine. The thus-prepared nitrophenylpyrimidine is in turn reduced with stannous chloride dihydrate in 10M hydrochloric acid, yielding the corresponding 2,4-diamino-6-ethyl-5-(3-amino-4chlorophenyl)pyrimidine. The aminophenylpyrimidine may then be treated with an appropriate acid chloride in the presence of a base in tetrahydrofuran, yielding the desired 2,4-diamino-6-ethyl-5-[3-(substituted1 5 carbonylamino)-4-chlorophenyl]pyrimidine (wherein W is -NHC(O)R⁴).

These compounds may optionally be reduced with 1M borontetrahydrofuran complex in tetrahydrofuran, affording 2,4-diamino-6-ethyl-5[3-(substituted-amino)-4-chlorophenyl]pyrimidine (wherein W is -NHR⁵).

Example 15 teaches a method of adding a substituent to the

0 pyrimidine-containing molecule after the pyrimidine ring is formed.

Example 16 teaches a method of preparing a hydrochloride salt of a (. previously prepared pyrimidine.

Example 17 is similar to the method taught by Example 5, however Example 17 is an improvement over Example 5 in that it saves two syn2 5 thesis steps in the preparation of the optionally substituted alkanenitriie intermediate. Example 17 also teaches a new method of forming the pyrimidine ring which is an improvement over that taught in Examples 2 and

5. This method replaces guanidine hydrochloride and sodium carbonate/DMF with guanidine carbonate and N,N-dimethylacetamide

0 (compare Example 2, Step E, with Example 17, Step E).

Example 18 is similar to the method taught by Example 12; however, Example 18 teaches a preparation for those compounds wherein m and p are both 1, as depicted in the structure:

AP . 0 0 5 0 3

-13NHz

C'

0

C'

0 and the remaining substituents are as defined above.

Example 19 is similar to the method taught in Example 12. The compound, 2,4-diamino-5-(adamant-1-yl)-6-methylpyrimidine, prepared by the method of Example 19, is active in various tests, as shown below.

Examples 20 - 28 specifically disclose some of the preferred compounds, i.e., those compounds with a plso value of > 5.7 in the Diet test.

Examples 29 and 30 are similar to the method taught by Examples 4, 5, and 17; however, Examples 29 and 30 teach a preparation for those compounds wherein R is a substituted phenyl lower alkyl moiety, as depicted in the following structure:

t i

AP/P/ 9 3 / 0 0 5 9 5 where q is 1.

Using the method taught in Example 29, a tertiary benzylic alcohol, for example 5-hydroxy-5-(3-trifluoromethylphenyl)hexanenitrile, was reduced to the corresponding arylalkanenitrile by the reaction of the alcohol with a chlorotrimethylsilane-sodium iodide-acetonitrile reagent in hexane. The arylalkanenitrile, for example, 5-(3-trifluoromethylphenyl)-hexanenitrile, was then reacted further by methods previously described to obtain the corresponding substituted-pyrimidine.

The following examples, which disclose the preparation of representative compounds of this invention (Table 1), are for the purpose of illustrating known methods for the preparation of the compounds employed in the methods and formulations of this invention.

AP. 00503

-14EXAMPLE1

Synthesis of 2,4-Diamino-5-[3-(naphth1 -yl)propyl]pyrimidine (Compound 47)

Step A Synthesis of 3-(naphth-1-yl)propyl iodide as an intermediate

Under a nitrogen atmosphere a solution of 6.8 grams (0.040 mole) of

3-(naphth-1-yl)-1-propene was stirred at 25°C, and 14 mL (0.013 mole) of

1M borane-tetrahydrofuran complex was added dropwise. The reaction

C mixture was maintained at 25°C during the addition and for another hour upon completion of the addition. After this time 1 mL of methanol was added to destroy excess boron intermediates. Iodine, 7.7 grams (0.030 mole), was then added in one portion, followed by the dropwise addition of

10 mL of 3M methanolic sodium hydroxide. Upon completion of addition, the reaction mixture was stirred for five minutes and then was poured into a solution of 3.0 grams of sodium thiosulfate in 150 mL of water. The mixture was extracted with two 100 mL portions of methylene chloride. The combined extracts were dried with magnesium sulfate and filtered. The

0 filtrate was concentrated under reduced pressure to a residual oil. The residual oil was subjected to column chromatography on silica gel. Elution C was accomplished using petroleum ether. The eluate was concentrated under reduced pressure, yielding 4.0 grams of 3-(naphth-1 -yl)propyl iodide. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 1-ethoxy-2-cyano-5-(naphth-1-yl)-1-pentene as an intermediate

Under a nitrogen atmosphere a stirred solution of 1.5 mL (0.014

0 mole) of 3-ethoxyacrylonitrile in 45 mL of tetrahydrofuran was cooled to about -110°C, and 6.2 mL (0.015 mole) of 2.5M n- butyllithium in hexanes was added dropwise at a rate to keep the reaction mixture temperature below -65°C. Upon completion of addition, the reaction mixture was stirred for about ten minutes, and then a solution of 4.0 grams (0.014 mole) of 33 5 (naphth-l-yl)propyl iodide in 15 mL of tetrahydrofuran was added dropwise. Upon completion of addition, the reaction mixture was stirred at about -78°C

AP. 00503

-15for two hours. After this time the reaction mixture was allowed to warm to ambient temperature as it stirred for about 16 hours. The reaction mixture was concentrated under reduced pressure, yielding a dark residual oil. The oil was passed through a short column of silica gel using methylene chloride as the eluant. The eluate was concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel. Elution was accomplished using 2:1 methylene chloride and petroleum ether. The appropriate fractions were combined and concentrated under reduced pressure, yielding 1.0 gram of 1-ethoxy-21 0 cyano-5-(naphth-1-yl)-1-pentene. The NMR spectrum was consistent with

C the proposed structure.

Step C Synthesis of 2,4-diamino-5-[3-(naphth-1-yl)-propyl]pyrimidine (Compound 47)

Under a nitrogen atmosphere a stirred solution of 0.85 gram (0.003 mole) of 1-ethoxy-2-cyano-5-(naphth-1-yl)-1 -pentene, 1.22 grams (0.013 mole) of guanidine hydrochloride, and 2.65 grams (0.019 mole) of potassium carbonate in 20 mL of N,N-dimethylformamide was heated at

110°C for 20 hours. After this time the reaction mixture was analyzed by thin layer chromatography (TLC) which indicated the reaction was not

(. complete. The reaction mixture was warmed to 125°C where it was stirred for six hours. TLC analysis of the reaction mixture after this time indicated ' that the reaction was still not complete. An additional 10 mL of

5 dimethylformamide was added, and the reaction mixture was stirred at

125°C for an additional 64 hours. The reaction mixture was then poured into water, and the mixture was extracted with two 100 mL portions of ethyl acetate. The combined extracts were concentrated under reduced pressure, yielding a yellow solid. The solid was triturated with

0 dichloromethane. The solid was collected by filtration and subjected to column chromatography on silica gel. Elution was accomplished with 10:1 methylene chloride and methanol. The eluate was concentrated under reduced pressure, yielding 0.30 gram of 2,4-diamino-5-[3-(naphth-1yl)propyl]pyrimidine, mp 179-181 °C. The NMR spectrum was consistent

5 with the proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-16EXAMPLE 2

Synthesis of 2,4-Diamino-5-(4-phenylbutyl)pyrimidine (Compound 37)

Step A Synthesis of 1 -methylsulfonyloxy-5-phenylpentane as an intermediate

A stirred solution of 25.0 grams (0.152 mole) of 5-phenylpentanol and 23.3 mL (0.167 mole) of triethylamine in 250 mL of methylene chloride was

0 cooled to 0°C, and a solution of 13.0 mL (0.167 mole) of methanesulfonyl C chloride in 50 mL of methylene chloride was added dropwise at a rate to maintain the reaction mixture temperature at 0°C to 5°C. The complete addition required about 45 minutes. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature. After this

5 time the reaction mixture was washed with two 200 mL portions of an aqueous 5% sodium bicarbonate solution and then with 200 mL of an aqueous solution saturated with sodium chloride. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 36.8 grams of 1 -methylsulfonyloxy-52 0 phenylpentane. The NMR spectrum was consistent with the proposed structure.

<

Step B Synthesis of 6-phenylhexanenitrile as an intermediate

A stirred solution of 15.0 grams (0.0062 mole) of 1 methylsulfonyloxy-5-phenyipentane and 9.1 grams (0.190 mole) of sodium cyanide in 150 mL of Ν,Ν-dimethylformamide was heated at 50°C to 55°C for about 60 hours. After this time the reaction mixture was poured into 400 mL of water. The mixture was extracted with three 250 mL portions of

0 diethyl ether. The combined extracts were then washed with three 200 mL portions of water and 200 mL of an aqueous solution saturated with sodium chloride. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 10.2 grams of 6-phenylhexanenitriie. The NMR spectrum was consistent with the

5 proposed structure.

AP . 0 0 5 0.3

-17Step C Synthesis of 2-cyano-6-phenyl-1-hexen-1-ol as an intermediate

A stirred solution of 2.2 mL (0.016 mole) of diisopropylamine in 200 mL of tetrahydrofuran was cooled to -80°C, and 6.4 mL (0.016 mole) of n5 butyllithium (2.5 Molar in hexanes) was added dropwise during a 15 minute period. Upon completion of addition, the reaction mixture was stirred for an additional 15 minutes. While still maintaining the reaction mixture temperature at -80°C to -75°C, a solution of 2.5 grams (0.016 mole) of 6phenylhexanenitrile in 50 mL of tetrahydrofuran was added dropwise

0 during a 30 minute period. Upon completion of addition, the reaction C mixture was stirred at -80°C for an additional 30 minutes. After this time, a solution of 1.3 mL (0.016 mole) of ethyl formate in 50 mL of tetrahydrofuran was added dropwise during a ten minute period. Upon completion of addition, the reaction mixture was stirred for two hours at -80°C to -70°C.

5 The reaction was quenched with 100 mL of water, and the mixture was acidified with 6N hydrochloric acid. The mixture was extracted with two 150 mL portions of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residue. The residue was subjected to column chromatography using silica gel. Elution was accomplished using 1% to 3% methanol in methylene chloride. The appropriate fractions were < combined and concentrated under reduced pressure, yielding 2.7 grams of ϊ 2-cyano-6-phenyl-1-hexen-1-ol. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 1-methoxy-2-cyano-6-phenyl-1-hexene as an intermediate

A solution of 3.0 grams of potassium hydroxide in 6 mL of water was

0 placed in a commercially available diazomethane generator. Ethanol, 8 mL, was added to the generator, and the contents of the generator were slowly warmed to 60°C to 70°C. To this was added dropwise a solution of

4.6 grams (0.021 mole) of Diazald® in a minimum amount of diethyl ether. The diazomethane generated was condensed on a cold finger and, in turn,

5 was allowed to drip into a stirred, cooled solution of 2.5 grams (0.012 mole) of 2-cyano-6-phenyl-1-hexen-1-ol in a minimum of diethyl ether. The

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-18complete generation of diazomethane required about 30 to 45 minutes. Upon completion of the diazomethane generation, the reaction mixture was allowed to warm to ambient temperature. After this time the reaction mixture was concentrated under reduced pressure, yielding 2.4 grams of 15 methoxy-2-cyano-6-phenyl-1 -hexene. The NMR spectrum was consistent with the proposed structure.

Diazald® (N-methyl-N-nitroso-p-toluenesulfonamide) from Aldrich Chemical Company, Inc., Milwaukee, Wisconsin.

C Step E Synthesis of 2,4-diamino-5-(4-phenylbutyl)pyrimidine

A stirred solution of 2.4 grams (0.011 mole) of 1-methoxy-2- cyano-6phenyi-1-hexene, 4.2 grams (0.044 mole) of guanidine hydrochloride, and

5 7.6 grams (0.055 mole) of potassium carbonate in 40 mL of Ν,Νdimethylformamide was heated at 100°C to 105°C for about 20 hours. After this time the reaction mixture was diluted with 100 mL of water. The mixture was then extracted with two 100 mL portions of ethyl acetate. The combined extracts were washed with three 75 mL portions of an aqueous

0 10% lithium chloride solution. The organic layer was dried with sodium sulfate and filtered. The filtrate was concentrated under reduced pressure ( to a residual oil. The residue was subjected to column chromatography on

- basic alumina that was deactivated to Level III with water. Elution was accomplished with 1% to 3% methanol in methylene chloride. The

5 appropriate fractions were combined and concentrated under reduced pressure, yielding 1.4 grams of 2,4-diamino-5-(4-phenylbutyl)pyrimidine; mp 126-127°C. The NMR spectrum was consistent with the proposed product.

0 EXAMPLE 3

SYNTHESIS OF 2,4-DIAMINO-5-[5-(NAPHTH1 -YL)PENTYL]PYRIMIDINE (Compound 54)

5 Step A Synthesis of 5-(naphth-1-yl)-4-pentyn-1-ol as an intermediate

AP. Ο η 5 Ο 5

-19Under a nitrogen atmosphere, a mixture of 4.0 mL (0.027 mole) of 1iodonaphthalene, 0.1 gram (0.0003 mole) of copper(l) iodide, and 0.19 gram (0.0003 mole) of bis(triphenylphosphine)palladium(ll) chloride in 75 mL of diethylamine was stirred, and 2.6 mL (0.027 mole) of 4-pentyn-1-ol was added in one portion. Upon completion of addition, the reaction mixture was stirred at ambient temperature for about 18 hours. Gas chromatographic (GC) analysis of the reaction mixture indicated that the reaction had not gone to completion. An additional 0.5 mL of the 4-pentyn1 -ol was added, and the reaction mixture was stirred for an additional six

0 hours. GC analysis of the reaction mixture indicated that the reaction was

Γ complete. The reaction mixture was passed through a column of silica gel.

Elution was accomplished using methylene chloride. The eluate was concentrated under reduced pressure, yielding 3.9 grams of 5-(naphth-1yl)-4-pentyn-1-ol. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 5-(naphth-1-yl)pentanol as an intermediate

A mixture of 3.5 grams (0.017 mole) of 5-(naphth-1-yl)-4-pentyn-1-ol

0 and 0.5 gram (catalyst) of 10% palladium on carbon in 30 mL of ethanol was shaken in a Parr hydrogenator until the theoretical amount of hydrogen ( gas was taken up. The reaction mixture was filtered to remove the catalyst, ( and the filtrate was concentrated under reduced pressure, yielding 3.4 grams of 5-(naphth-1-yl)pentanol. The NMR spectrum was consistent with

5 the proposed structure.

Step C Synthesis of 1-methylsulfonyloxy-5-(naphth-1-yl)pentane as an intermediate

0 This compound was prepared in a manner analogous to that of

Example 2, Step A, using 3.1 grams (0.015 mole) of 5-(naphth-1yl)pentanol, 2.1 mL (0.015 mole) of triethylamine, and 1.2 mL (0.015 mole) of methanesulfonyl chloride. The yield of 1-methylsulfonyloxy-5-(naphth-1yljpentane was 4.1 grams. The NMR spectrum was consistent with the

5 proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

AP.00503

-20Step D Synthesis of 5-(naphth-1-yl)pentyl iodide as an intermediate

Under a nitrogen atmosphere, a stirred solution of 4.1 grams (0.014 mole) of 1-methylsulfonyloxy-5-(naphth-1-yl)pentane, and 4.2 grams (0.028 mole) of sodium iodide in 150 mL of acetone was heated at reflux for 2.5 hours. The reaction mixture was poured into 400 mL of water, and the mixture was extracted with two 100 mL portions of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 3.9 grams of 51 0 (naphth-1 -yl)pentyl iodide. The NMR spectrum was consistent with the C proposed structure.

Step E Synthesis of 1-ethoxy-2-cyano-7-(naphth-1-yl)-1-heptene as an intermediate

5

This compound was prepared in a manner analogous to that of Example 1, Step B, using 3.9 grams (0.012 mole) of 5-(napth-1-yl)pentyl iodide, 1.4 mL (0.013 mole) of 3-ethoxyacrylonitrile and 5.3 mL (0.013 mole) of n-butyllithium (2.5 M in hexanes) in 75 mL of tetrahydrofuran. The yield of 1-ethoxy-2-cyano-7-(naphth-1-yl)-1-heptene was 1.3 grams. The NMR spectrum was consistent with the proposed structure.

c

Step F Synthesis of 2,4-diamino-5-[5-(naphth-1-yl)pentyl]pyrimidine

5 This compound was prepared in a manner analogous to that of

Example 2, Step E, using 1.3 grams (0.004 mole) of 1-ethoxy-2-cyano-7(naphth-1-yl)-1-heptene, 1.7 grams (0.018 mole) of guanidine hydrochloride, and 3.6 grams (0.026 mole) of potassium carbonate in 25 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5-[5-(naphth-13 0 yl)pentyl]pyrimidine was 0.8 gram, mp 128-133°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 4

SYNTHESIS OF 2,4-DIAMINO-5-[3-(3-PHENYLPHENYL)PROPYL]6-ETHYLPYRIMIDINE (Compound 33)

AP.00503

-21 Step A Synthesis of ethyl 3-(3-bromophenyl)-2-propenoate as an intermediate

Under a nitrogen atmosphere, a stirred solution of 10.1 grams (0.045 mole) of 3-bromocinnamic acid and 40 drops of concentrated sulfuric acid in 75 mL of ethanol was heated at reflux for about 19 hours. After this time the reaction mixture was concentrated under reduced pressure to a residual oil. The oil was dissolved in methylene chloride, and the solution was

0 washed with 50 mL of water and then with 50 mL of an aqueous solution f saturated with sodium bicarbonate. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding about 11.4 grams of ethyl 3-(3-bromophenyl)-2propenoate. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of ethyl 3-(3-phenylphenyl)-2-propenoate as an intermediate

A stirred mixture of 11.0 grams (0.043 mole) of ethyl 3-(3bromophenyl)-2-propenoate, 5.8 grams (0.047 mole) of phenylboronic acid, ( 0.25 gram (catalyst) of tetrakis-(triphenylphosphine)palladium(0), and 54.0 mL (0.110 mole) of a 2M solution of aqueous sodium carbonate in 100 mL of toluene was heated at reflux during a two hour period. After this time the

5 reaction mixture was cooled, and the aqueous layer was separated. The toluene layer was washed with two 100 mL portions of water and then with 100 mL of an aqueous solution saturated with sodium chloride. The toluene layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 12.0 grams of ethyl 3. 3 0 (3-phenylphenyl)-2-propenoate. The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of ethyl 3-(3-phenylphenyl)propanoate as an intermediate

AP/P/ 9 3 / 0 0 5 9 5

AP. Ο η 5 ο 3

-22This compound was prepared in a manner analogous to that of Example 3, Step B, using 11.8 grams (0.047 mole) of ethyl 3-(3phenylphenyl)-2-propenoate, hydrogen gas, 0.5 gram (catalyst) of 5% palladium on carbon, 25 mL of ethanol and 25 mL of ethyl acetate. The yield of ethyl 3-(3-phenyl-phenyl)propanoate was 10.8 grams. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 3-oxo-5-(3-phenylphenyl)pentanenitrile as an intermediate

Γ ζ' (

Under a nitrogen atmosphere, a stirred solution of 2.3 mL (0.044 mole) of acetonitrile in 50 mL of tetrahydrofuran was cooled to about -80°C, and 17.7 mL (0.044 mole) of n-butyllithium (2.5M in hexanes) was added dropwise at a rate to maintain the reaction mixture temperature below -75°C. Upon completion of addition, the reaction mixture was stirred at -78°C for 30 minutes. After this time a solution of 10.3 grams (0.040 mole) of ethyl 3-(3-phenylphenyl)propanoate in 100 mL of tetrahydrofuran was added dropwise at a rate to maintain the reaction mixture temperature below -75°C. Upon completion of addition, the reaction mixture was stirred at -78°C for one hour. After this time the reaction mixture was allowed to warm to ambient temperature, then was cooled to about 0°C, and 30 mL of aqueous 6N hydrochloric acid was added dropwise. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature where it was stirred for 30 minutes. The mixture was poured into 200 mL of water, and then it was extracted with two 100 mL portions of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residual oil. NMR analysis of the residual oil indicated that it was impure. The oil was dissolved in 10:1 methylene chloride and acetone, and the solution was passed through a column of basic alumina that was deactivated to Level III with water. The eluate was concentrated under reduced pressure, yielding

5.9 grams of 3-oxo-5-(3-phenylphenyl)pentanenitrile. The NMR spectrum was consistent with the proposed structure.

Step E Synthesis of 3-hydroxy-5-(3-phenylphenyl)pentanenitrile as an intermediate

AP. Ο Ο 5 Ο 3

-23Under a nitrogen atmosphere a stirred solution of 5.5 grams (0.022 mole) of 3-oxo*5-(3-phenylphenyl)pentanenitrile and 0.5 gram (0.013 mole) of sodium borohydride in 75 mL of tetrahydrofuran was heated at reflux for four hours. After this time the reaction mixture was allowed to cool to ambient temperature where it stood for about 16 hours. The reaction mixture was then poured into 300 mL of aqueous 1N hydrochloric acid. The mixture was extracted with two 150 mL portions of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The

0 filtrate was concentrated under reduced pressure to a residual oil. NMR C analysis of the residual oil indicated that it was impure. The oil was dissolved in 20:1 methylene chloride and ethyl acetate, and the solution was passed through a column of silica gel. The eluate was concentrated under reduced pressure, yielding 3.8 grams of 3-hydroxy-5-(3-phenyl1 5 phenyl)pentanenitrile. The NMR spectrum was consistent with the proposed structure.

Step F Synthesis of 3-methylsulfonyloxy-5-(3- phenylphenyl)pentanenitrile as an intermediate

This compound was prepared in a manner analogous to that of ( Example 2, Step A, using 3.4 grams (0.014 mole) of 3-hydroxy-5-(3phenylphenyl)pentanenitrile, 1.1 mL (0.014 mole) of methanesulfonyl (· chloride, and 1.9 mL (0.014 mole) of triethylamine in 100 mL of ethylene

5 chloride. The yield of 3-methylsulfonyloxy-5-(3-phenylphenyl) pentanenitrile was 3.4 grams. The NMR spectrum was consistent with the proposed structure.

Step G Synthesis of 5-(3-phenylphenyl)pentanenitrile as an intermediate 30

A stirred mixture of 3.4 grams (0.010 mole) of 3-methylsulfonyloxy-5(3-phenylphenyl)pentanenitrile, 3.4 grams (0.023 mole) of sodium iodide,

3.4 grams (0.052 mole) of zinc powder, and 3.4 mL of water in 50 mL of ethylene glycol dimethyl ether was heated at reflux for about 22 hours. The

5 reaction mixture was cooled, and 150 mL of diethyl ether was added. The mixture was filtered, and the filtrate was washed in succession with water,

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-24an aqueous 5% hydrochloric acid solution, an aqueous 5% sodium bicarbonate solution, an aqueous 5% sodium bicarbonate solution, an aqueous 5% sodium thiosulfate solution, and finally with water. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residual oil. NMR analysis of the residual oil indicated that it was impure. The oil was dissolved in 10:1 petroleum ether and diethyl ether, and the solution was passed through a column of silica gel. Early fractions of eluate were combined and concentrated under reduced pressure, yielding an intermediate product, 510 (3-phenylphenyl)-2-pentenenitrile. Later fractions of the eluate were C combined and concentrated under reduced pressure, yielding the intended product, 5-(3-phenylphenyl)pentanenitrile. The intermediate was hydrogenated in a manner analogous to that of Example 3, Step B, yielding an additional amount of 5-(3*phenylphenyl)pentanenitrile. All samples of the pentanenitrile were combined, yielding 1.4 grams of that product. The NMR spectrum was consistent with the proposed structure.

Step H Synthesis of 1 -(3-phenylphenyl)-4-cyano-5-heptanone as an intermediate

This compound was prepared in a manner analogous to that of Step D, of this Example, using 1.4 grams (0.006 mole) of 5-(3-phenylphenyl)pentanenitrile, 0.8 mL (0.007 mole) of ethyl propanoate, and 2.8 mL (0.007

- mole) of n-butyllithium (2.5M in hexanes) in 50 mL of tetrahydrofuran. The yield of 1-(3-phenylphenyl)-4-cyano-5-heptanone was 1.0 gram. The NMR spectrum was consistent with the proposed structure.

Step I Synthesis of 1-{3-phenylphenyl)-4-cyano-5-methoxy-4-heptene as an intermediate

This compound was prepared in a manner analogous to that of Example 2, Step D, using 1.0 gram (0.004 mole) of 1 -(3-phenylphenyl)-4cyano-5-heptanone, 1.3 grams (0.006 mole) of Diazald, 3.5 grams (0.063 mole) of potassium hydroxide, 7 mL of water and 12 mL of ethanol. The

5 yield of 1-(3-phenylphenyl)-4-cyano-4-methoxy-4-heptene was 1.0 gram.

The NMR spectrum was consistent with the proposed structure.

AP. 00503

-25Step J Synthesis of 2,4-diamino-5-[3-(3-phenylphenyl)propyl]-6-ethylpyrimidine

This compound was prepared in a manner analogous to that of

Example 1, Step C, using 1.0 gram (0.003 mole) of 1-(3-phenylphenyl)-4cyano-5-methoxy-4-heptene, 1.3 grams (0.013 mole) of guanidine hydrochloride, and 2.7 grams (0.020 mole) of potassium carbonate in 15 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5-[3-(310 phenylphenyl)propyl]-6-ethylpyrimidine was 0.3 gram, mp 128-133°C. The C NMR spectrum was consistent with the proposed structure.

EXAMPLE 5

SYNTHESIS OF 2,4-DIAMINO-5-[3-(2,4,5,-TRICHLOROPHENYL)15 PROPYLJ-6-ETHYLPYRIMIDINE (Compound 34)

Step A Synthesis of 4-(2,4,5-trichlorophenyl)-3-butyn-1-ol as an intermediate

This compound was prepared in a manner analogous to that of 0 Example 3, Step A, using 10.0 grams (0.033 mole) of 2,4,5trichloroiodobenzene, 2.5 mL (0.033 mole) of 3-butyn-1-ol, 0.23 gram (0.0003 mole) of bis(triphenylphosphine)palladium(ll) chloride and 0.1

5 gram (0.0003 mole) of copper(ll) iodide in 100 mL of diethylamine. The yield of 4-(2,4,5-trichlorophenyl)-3-butyn-1-ol was 7.3 grams. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 4-(2,4,5-trichlorophenyl)butanol as an intermediate 30

This compound was prepared in a manner analogous to that of Example 3, Step B, using 7.0 grams (0.0030 mole) of 4-(2,4,5trichlorophenyl)-3-butyn-1-ol, hydrogen gas, and 0.25 gram (catalyst) of 10% palladium on carbon in 50 mL of ethanol. The yield of 4-(2,4,53 5 trichlorophenyl)butanol was 7.0 grams. The NMR spectrum was consistent with the proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-26Step C Synthesis of 1-methylsulfonyloxy-4-(2,4,5-trichlorophenyl)butane as an intermediate

This compound was prepared in a manner analogous to that of

Example 2, Step A, using 6.6 grams (0.026 mole) of 4-(2,4,5trichiorophenyl)butanol, 2.0 mL (0.026 mole) of methanesulfonyl chloride, and 3.6 mL of triethylamine in 75 mL of methylene chloride. The yield of 1methylsulfonyloxy-4-(2,4,5-trichlorophenyl)butane was 7.9 grams. The

NMR spectrum was consistent with the proposed structure.

c

Step D Synthesis of 5-(2,4,5-trichlorophenyl)pentanenitrile as an intermediate

5 This compound was prepared in a manner analogous to that of

Example 2, Step B, using 7.9 grams (0.024 mole) of 1 -methylsulfonyloxy-4(2,4,5-trichlorophenyl)butane and 3.5 grams (0.072 mole) of sodium cyanide in 50 mL of Ν,Ν-dimethylformamide. The yield of 5-(2,4,5trichlorophenyl)pentanenitrile was 6.1 grams. The NMR spectrum was

0 consistent with the proposed structure.

θ Step E Synthesis of 1-(2,4,5-trichlorophenyl)-4-cyano-5-heptanone as an intermediate

5 This compound was prepared in a manner analogous to that of

Example 4, Step D, using 5.8 grams (0.022 mole) of 5-(2,4,5trichlorophenyl)pentanenitrile, 2.8 mL (0.024 mole) of ethyl propanoate, and

9.8 mL (0.024 mole) of n-butyllithium (2.5M in hexanes) in 100 mL of tetrahydrofuran. The yield of 1-(2,4,5-trichlorophenyl)-4-cyano-53 0 heptanone was 7.1 grams. The NMR spectrum was consistent with the proposed structure.

Step F Synthesis of 1-(2,4,5-trichlorophenyl)-4-cyano-5-methoxy-4heptene as an intermediate

AP. Ο Ο 5 Ο 3

-27This compound was prepared in a manner analogous to that of Example 2, Step D, using 7.1 grams (0.022 mole) of 1-(2,4,5trichlorophenyl)-4-cyano-5-heptanone, 8.2 grams (0.037 mole) of Diazald, 10.0 grams (0.175 mole) of potassium hydroxide, 16 mL of water, and 20 mL of ethanol. The yield of 1-(2,4,5-trichlorophenyl)-4-cyano-5-methoxy-4heptene was 7.2 grams. The NMR spectrum was consistent with the proposed structure.

Step G Synthesis of 2,4-diamino-5-[3-(2,4,5-trichlorophenyl)propyl]-610 ethylpyrimidine r

This compound was prepared in a manner analogous to that of Example 2, Step E, using 6.6 grams (0.020 mole) of 1-(2,4,5trichlorophenyl)-4-cyano-5-methoxy-4-heptene, 7.6 grams (0.08 mole) of

5 guanidine hydrochloride, and 16.6 grams (0.12 mole) of potassium carbonate in 50 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5[3-(2,4,5-trichlorophenyl)propyl]-6-ethylpyrimidine was 0.9 gram, mp 168182°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 6

SYNTHESIS OF 2,4-DIAMINO-5-[3-(4-METHOXYPHENYL)C PROPYLJPYRIMIDINE (Compound 32)

5 Step A Synthesis of 3-(4-methoxyphenyl)propyl iodide as an intermediate

Under a nitrogen atmosphere, a solution of 8.0 grams (0.048 mole) of

3-(4-methoxyphenyl)propanol and 7.9 grams (0.053 mole) of sodium iodide

0 in 50 mL of acetone was stirred, and 5.7 mL (0.053 mole) of 1-chloroethyl chloroformate was slowly added. Upon completion of addition, the reaction mixture was heated to reflux where it was stirred for four hours. After this time the reaction mixture was cooled, and 50 mL of toluene was added.

The acetone was removed by distillation after which the reaction mixture

5 was heated for one hour at reflux in the toluene. The reaction mixture was then cooled and poured into 300 mL of water. The mixture was extracted

AP/P/ 93/00595

AP . Ο Ο 5 Ο 3

-28with 200 mL of diethyl ether. The ether extract was washed with 100 mL of an aqueous 5% sodium thiosulfate solution and then with water. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residual oil, and the oil was dissolved in methylene chloride and filtered through a pad of silica gel. The filtrate was concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel. Elution was accomplished with 40:1 petroleum ether and diethyl ether. The appropriate fractions were combined and concentrated under reduced pressure,

0 yielding 4.3 grams of 3-(4-methoxyphenyl)propyl iodide. The NMR

C spectrum was consistent with the proposed structure.

Step B Synthesis of 1-ethoxy-2-cyano-5-(4-methoxyphenyl)-1-pentene as an intermediate

This compound was prepared in a manner analogous to that of Example 1, Step B, using 4.2 grams (0.015 mole} of 3-(4-methoxyphenyl) propyl iodide, 1.7 mL (0.017 mole) of 3-ethoxyacrylonitrile, and 6.7 mL of nbutyllithium (2.5M in hexanes) in 75 mL of tetrahydrofuran. The yield of 1 2 0 ethoxy-2-cyano-5-(4-methoxyphenyl)-1 -pentene was 0.8 gram. The NMR spectrum was consistent with the proposed structure.

c

Step C Synthesis of 2,4-diamino-5-[3-(4-methoxyphenyl)propyljpyrimidine

This compound was prepared in a manner analogous to that of Example 2, Step E, using 0.8 gram (0.003 mole) of 1-ethoxy-2-cyano-5-(4methoxyphenyl)-1-pentene, 1.3 grams (0.012 mole) of guanidine hydrochloride, and 2.7 grams (0.018 mole) of potassium carbonate in 15

0 mL of Ν,Ν-dimethylformamide. The yield of 1,4-diamino-5-[3-(4methoxyphenyl)propyl]pyrimidine was 0.5 gram, mp 151-154°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 7

SYNTHESIS OF 2,4-DIAMINO-5-(5-PHENYLPENTYL)-6-(4-PHENYLBUTYL)PYRIMIDINE

AP. ο ο 5 Ο 3

-29(Compound 43)

Step A Synthesis of ethyl 5-phenylpentanoate as an intermediate

This compound was prepared in a manner analogous to that of

Example 4, Step A, using 19.9 grams (0.112 mole) of 5-phenylpentanoic acid and 15 drops of concentrated sulfuric acid in 150 mL of ethanol. The yield of ethyl 5-phenylpentanoate was 22.0 grams. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 1,11-diphenyl-6-cyano-5-undecanone as an intermediate

This compound was prepared in a manner analogous to that of 15 Example 2, Step C, using 8.4 grams (0.041 mole) ethyl 5-phenylpentanoate, 7.7 grams (0.041 mole) of 7-phenylheptanenitrile (prepared in a manner analogous to that of Example 2, Steps A and B), 5.8 mL (0.041 mole) of diisopropylamine, and 16.4 mL (0.041 mole) of n-butyllithium (2.5M in hexanes) in about 250 mL of tetrahydrofuran. The yield of 1,11-diphenyl2 0 6-cyano-5-undecanone was 6.4 grams. The NMR spectrum was consistent with the proposed structure.

('

Step C Synthesis of 1,1l-diphenyl-5-methoxy-6-cyano-5-undecene as ⁽ an intermediate

This compound was prepared in a manner analogous to that of Example 2, Step D, using 6.4 grams (0.018 mole) of 1,11-diphenyl-6-cyano5-undecanone, 6.8 grams (0.032 mole) of Diazald, 7.5 grams (0.132 mole) of potassium hydroxide, 12 mL of water, and 16 mL of ethanol. The yield of

0 1,11-diphenyl-5-methoxy-6-cyano-5-undecene was 6.7 grams. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 2,4-diamino-5-(5-phenylpentyl)-6-(4-phenylbutyl)pyrimidine

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-30This compound was prepared in a manner analogous to that of Example 2, Step E, using 6.4 grams (0.018 mole) of 1,11-diphenyl-5methoxy-6-cyano-5-undecene, 6.7 grams (0.070 mole) of guanidine hydrochloride, and 12.1 grams (0.090 mole) of potassium carbonate in 50 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5-(5-phenylpentyl)-6-(4-phenylbutyl)pyrimidine was 1.1 grams; mp 75-78°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 8

0 SYNTHESIS OF 2,4-DIAMINO-5-(3C PHENYLPHENYLMETHYL)PYRIMIDINE (Compound 22)

Method A Synthesis of 3-(3-phenylphenyl)propanenitrile as an 15 intermediate

Step A Synthesis of 3-phenylphenyl methanol as an intermediate

This compound was prepared in a manner analogous to that of 20 Example 4, Step B, using 10.0 grams (0.054 mole) of 3- bromophenylmethanol, 7.2 grams (0.059 mole) of phenylboronic acid, 0.25 gram C (catalyst) of tetrakis(triphenylphosphine)palladium(0), and 66 mL (0.135 mole) of aqueous 2M sodium carbonate in 50 mL of toluene. The crude product from this reaction was combined with a previous smaller run

5 conducted on 4.6 grams (0.020 mole) of 3-bromophenylmethanol. The combination was subjected to column chromatography on silica gel.

Elution was accomplished using 20.Ί methylene chloride and ethyl acetate. The appropriate fractions were combined and concentrated under reduced pressure, yielding 12.9 grams of 3-phenylphenylmethanol. The NMR

0 spectrum was consistent with the proposed structure.

Step B Synthesis of 3-phenylphenylmethyl bromide as an intermediate

Under a nitrogen atmosphere a stirred solution of 12.9 grams of 33 5 phenylphenylmethanol in 50 mL of 47-49% hydrobromic acid was heated at reflux for two hours. Thin layer chromatographic analysis of the reaction

AP. 00503

-31 mixture indicated that the reaction was incomplete. An additional 50 mL of 47-49% hydrobromic acid was added, and the stirred reaction mixture was heated at reflux for an additional two hours. After this time the reaction mixture was poured into ice-water, and the mixture was extracted with 100 mL of diethyl ether. The extract was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding

16.2 grams of 3-phenylphenylmethyl bromide. The NMR spectrum was consistent with the proposed structure.

0 Step C Synthesis of 3-(3-phenylphenyl)propanenitrile as an intermediate C

This compound was prepared in a manner analogous to that of Example 4, Step D, using 6.0 grams (0.024 mole) of 3-phenylphenylmethyl bromide, 2.5 mL (0.048 mole) of acetonitrile, and 19.5 mL (0.048 mole) of n1 5 butyllithium (2.5M in hexanes) in 75 mL of tetrahydrofuran. The yield of 3(3-phenylphenyl)propanenitrile was 0.6 gram. The NMR spectrum was consistent with the proposed structure. The reaction was repeated in the manner of Method B, below.

0 Method B Synthesis of 3-(3-phenylphenyl)propanenitrile as an intermediate c

Step A Synthesis of 3-phenylbenzaldehyde as an intermediate

5 This compound was prepared in a manner analogous to that of

Example 4, Step B, using 7 mL (0.060 mole) of 3-bromobenzaldehyde, 8.1 grams (0.066 mole) of phenylboronic acid, 0.25 gram (catalyst) of tetrakis(triphenylphosphine)palladium(0), and 75 mL (0.150 mole) of aqueous 2M sodium carbonate in 50 mL of toluene. The yield of 33 0 phenylbenzaldehyde was 9.4 grams. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 3-hydroxy-3-(3-phenylphenyl)propanenitrile as an intermediate

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-32This compound was prepared in a manner analogous to that of Example 4, Step D, using 9.4 grams (0.052 mole) of 3-phenylbenzaldehyde, 3.0 mL (0.057 mole) of acetonitrile, and 22.7 mL (0.057 mole) of n-butyllithium (2.5M in hexanes) in 100 mL of tetrahydrofuran. The yield of

3-hydroxy-3-(3-phenylphenyl)propanenitrile was 10.7 grams. The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of 3-(3-phenylphenyl)-2-propenenitrile as an intermediate

C Under a nitrogen atmosphere a mixture of 1.9 grams (0.048 mole) of sodium hydride (60% in mineral oil) in 50 mL of tetrahydrofuran was stirred, and a solution of 10.7 grams (0.048 mole) of 3-hydroxy-3-(3phenylphenyl)propanenitrile in 100 mL of tetrahydrofuran was added

5 dropwise. Upon completion of addition, the reaction mixture was stirred until the evolution of hydrogen gas ceased. After this time the reaction mixture was cooled in an ice-bath, and 4.6 mL (0.048 mole) of acetic anhydride was added dropwise. Upon completion of addition, the reaction was allowed to warm to ambient temperature where it was stirred for about

18 hours. The reaction mixtOre was poured into 300 mL of water, and the mixture was extracted with 200 mL of diethyl ether. The extract was dried

C. with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel using methylene chloride as an eluant. The

5 appropriate fractions were combined and concentrated under reduced pressure, yielding 4.0 grams of 3-(3-phenylphenyl)-2-propenenitrile. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 3-(3-phenylphenyl)propanenitrile as an intermediate 30

This compound was prepared in a manner analogous to that of Example 3, Step B, using 4.0 grams (0.020 mole) of 3-(3-phenylphenyl)-2propenenitrile, hydrogen gas, and 0.4 gram (catalyst) of 10% palladium on carbon in 30 mL of ethanol. The yield of 3-(3-phenylphenyl)propanenitrile

5 was 3.2 grams. The NMR spectrum was consistent with the proposed structure.

AP. 0 π 5 Ο 3

-33The 0.6 gram from Method A was combined with the 3.2 grams from Method B, giving 3.8 grams of 3-(3-phenylphenyl)propanenitrile for use in the next reaction designated Step A.

Step A Synthesis of 1-(3-phenylphenyl)-2-cyano-3-pentanone as an intermediate

This compound was prepared in a manner analogous to that of 10 Example 2, Step C, using 3.6 grams (0.017 mole) of 3-(3-phenylphenyl)

C propanenitrile, 2.0 mL (0.017 mole) of ethyl propanoate, 2.4 mL (0.017 mole) of diisopropylamine, and 6.9 mL (0.017 mole) of n-butyllithium (2.5M in hexanes) in 75 mL of tetrahydrofuran. The yield of 1-(3-phenylphenyl)-2cyano-3-pentanone was 1.5 grams. The NMR spectrum was consistent with

5 the proposed structure.

Step B Synthesis of 1-(3-phenylphenyl)-2-cyano-3-methoxy-2-pentene as an intermediate

0 This compound was prepared in a manner analogous to that of

Example 2, Step D, using 1.4 grams (0.005 mole) of 1-(3-phenylphenyl)-2C cyano-3-pentanone, 2.1 grams (0.010 mole) of Diazald, 2.5 grams (0.044 mole) of potassium hydroxide, 5 mL of water, and 8 mL of ethanol. The yield of 1-(3-phenylphenyl)-2-cyano-3-methoxy-2-pentene was 1.4 grams.

5 The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of 2,4-diamino-5-(3- phenylphenylmethyl)pyrimidine

This compound was prepared in a manner analogous to that of

0 Example 2, Step E, using 1.4 grams (0.005 mole) of 1-(3-phenylphenyl)-2cyano-3-methoxy-2-pentene, 2.0 grams (0.020 mole) of guanidine hydrochloride, and 4.2 grams (0.031 mole) of potassium carbonate in 15 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5-(3-phenylphenylmethyljpyrimidine was 0.6 gram; mp 108-115°C. The NMR spectrum

5 was consistent with the proposed structure.

AP/P' 9 3 / 0 0 5 9 5 ^ΑΡ 00503

-34EXAMPLE 9

SYNTHESIS OF 2-ETHYLAMINO-4-AMINO-5-(3-(NAPHTH-1-YL)PROPYLJPYRIMIDINE (Compound 50)

Step A Synthesis of ethyl 3-(naphth-1-yl)propanoate as an intermediate

A stirred solution of 50 grams (0.25 mole) of 3-(naphth-1-yl) propanoic acid and 20 drops (catalyst) of concentrated sulfuric acid in 250 mL of ethanol was heated at reflux for about 24 hours. After this time the reaction mixture was concentrated under reduced pressure to a residue. The residue was dissolved in diethyl ether, and the solution was washed with two 150 mL portions of an aqueous solution saturated with sodium bicarbonate and then with 150 mL of an aqueous solution saturated with

5 sodium chloride. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 56.0 grams of ethyl 3-{naphth-1-yl)propanoate. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 3-(naphth-1-yl)propanol as an intermediate ( Under a nitrogen atmosphere, a mixture of 1.3 grams (0.033 mole) of lithium aluminum hydride in 40 mL of diethyl ether was stirred for one hour. The mixture was then cooled, and a solution of 10.0 grams (0.044 mole) of

5 ethyl 3-(naphth-1-yl)propanoate in 35 mL of diethyl ether was added dropwise at a rate to keep the reaction mixture temperature below 25°C. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature where it was stirred for one hour. The reaction mixture was again cooled, and 50 mL of ethyl acetate was added to destroy excess

0 lithium aluminum hydride. After this time 30 mL of an aqueous 10% sodium hydroxide solution was added dropwise. The reaction mixture was then acidified with aqueous 6N hydrochloric acid, and then it was poured into 300 mL of water. The organic layer was separated, dried with magnesium sulfate, and filtered. The filtrate was concentrated under reduced pressure,

5 yielding 8.2 grams of 3-(naphth-1-yl)propanol. The NMR spectrum was consistent with the proposed structure.

^Λρ. ^ηη5η.τ

-35Step C Synthesis of 1-methylsulfonyloxy-3-(naphth-1-yl)propane as an intermediate

This compound was prepared in a manner analogous to that of

Example 2, Step A, using 8.2 grams (0.044 mole) of 3-(naphth-1yl)propanol, 3.4 grams (0.044 mole) of methanesulfonyl chloride, and 6.2 mL (0.044 mole) of triethylamine in 75 mL of methylene chloride. The yield of 1-methylsulfonyloxy-3-(naphth-1-yl)propane was 11.6 grams. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 3-(naphth-1-yl)propyl iodide as an intermediate

An alternate preparation of this compound is shown in Example 1,

5 Step A. In the present example, this compound was prepared in a manner analogous to that of Example 3. Step D, using 11.6 grams (0.044 mole) of 1-methylsulfonyloxy-3-(naphth-1-yl)propane, and 13.2 grams (0.088 mole) of sodium iodide in 250 mL of acetone. The yield of 3-(naphth-1-yl)propyl iodide was 12.1 grams. The NMR spectrum was consistent with the

0 proposed structure.

( Step E Synthesis of 1-ethoxy-2-cyano-5-(naphth-1-yl)-1-pentene as an intermediate

(.

5 This is the same compound as shown in Example 1, Step B. In the present example it was prepared in an analogous manner, using 12.1 grams (0.042 mole) of 3-(naphth-1-yl)propyl iodide, 48.0 mL (0.046 mole) of

3-ethoxyacrylonitrile, and 19.0 mL (0.046 mole) of n-butyllithium (2.5M in hexanes) in 200 mL of tetrahydrofuran. The yield of 1-ethoxy-2-cyano-530 (naphth-1-yl)-1 -pentene was 5.3 grams. The NMR spectrum was consistent with the proposed structure.

Step F Synthesis of 2-ethylamino-4-amino-5-[3-(naphth-1yl)propyl]pyrimidine

AP/P/ 93/00595

AP on so Λ

-36This compound was prepared in a manner analogous to that of Example 2, Step E, using 1.2 grams (0.005 mole) of 1-ethoxy-2-cyano-5(naphth-1 -yl)-1 -pentene, 2.2 grams (0.020 mole) of 1-ethylguanidine hydrochloride and 3.7 grams (0.03 mole) of potassium carbonate in 12 mL of Ν,Ν-dimethylformamide. The yield of 2-ethylamino-4-amino-5-[3(naphth-l-yl)propyljpyrimidine was 0.5 gram. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 10

SYNTHESIS OF 2.4-DIAMINO-5-PHENYLPYRIMIDINE (Compound 15)

Step A Synthesis of 1-cyano-1-phenyl-2-(N,N-dimethylamino)ethene as an intermediate

A stirred solution of 5.1 grams (0.044 mole) of benzyl cyanide and

8.7 grams (0.065 mole) of Ν,Ν-dimethylformamide dimethyl acetal in 150 mL of methanol was heated at reflux for about 20 hours. After this time gas chromatographic analysis of the reaction mixture indicated that the reaction was not complete. An additional 3.0 mL of the intermediate acetal (total 0.088 mole) was added, and the heating at reflux was continued an additional 24 hours. After this time the reaction mixture was concentrated under reduced pressure to a residue. The residue was triturated with 25% methylene chloride in petroleum ether, yielding 5.9 grams of 1-cyano-1phenyI-2-(N,N-dimethylamino)ethene. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 2,4-diamino-5-phenylpyrimidine

This compound was prepared in a manner analogous to that of Example 2, Step E, using 4.9 grams (0.031 mole) of 1-cyano-1-phenyl-2(N.N-dimethylamino)ethene, 11.8 grams (0.124 mole) of guanidine hydrochloride, and 21.4 grams (0.156 mole) of potassium carbonate in Ν,Νdimethylformamide. The yield of 2,4-diamino-5-phenylpyrimidine was 0.7 gram; mp 162-164’C. The NMR spectrum was consistent with the proposed structure.

AP.00503

-37EXAMPLE 11

SYNTHESIS OF 2,4,6-TRIAMINO-5-[3-(NAPHTH-1-YL) PROPYLJPYRIMIDINE (Compound 48)

Step A Synthesis of 2-cyano-5-(naphth-1-yl)pentanenitrile as an intermediate

A solution of 0.7 gram (0.011 mole) of malononitrile in 50 mL of tetrahydrofuran was stirred, and 0.5 gram (0.011 mole) of sodium hydride (60% in mineral oil) was added portionwise during a ten minute period. Upon completion of addition, the reaction mixture was stirred for 30 minutes at ambient temperature. After this time a solution of 3.0 grams (0.010 mole) of 3-(naphth-1-yl)propyl iodide (prepared as in Example 9, Steps A-D) in about 5 mL of tetrahydrofuran was added in one portion. Upon completion of addition, the reaction mixture was stirred at ambient temperature for about 20 hours. The reaction mixture was acidified with aqueous 6N hydrochloric acid and then was further diluted with 100 mL of water. The

0 mixture was extracted with two 100 mL portions of diethyl ether. The combined extracts were washed with an aqueous solution saturated with sodium chloride. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated to a residue which was subjected to

C column chromatography on silica gel. Elution was accomplished using

5 40% to 65% methylene chloride and petroleum ether. The appropriate fractions were combined and concentrated under reduced pressure, yielding 1.1 grams of 2-cyano-5-(naphth-1-yl)pentanenitrile. The NMR spectrum was consistent with the proposed structure.

0 Step B Synthesis of 2,4,6-triamino-5-[3-(naphth-1-yl)propyl]pyrimidine (Compound 48)

A solution of sodium ethoxide, prepared from 0.04 gram (0.002 mole) of sodium metal in 50 mL of ethanol, was stirred, and 0.2 gram (0.002 mole)

5 of guanidine hydrochloride was added in one portion. The reaction mixture was stirred for 15 minutes and filtered to remove a precipitate. The filtrate

AP/P/ 9 3 / 0 0 5 9 5

AP. On so j

-38was stirred, and 0.4 gram (0.002 mole) of 2-cyano-5-(naphth-1yl)pentanenitrile was added in one portion. Upon completion of addition, the reaction mixture was heated at reflux for about 16 hours. After this time the reaction mixture was concentrated under reduced pressure to a residue. The residue was triturated with warm water, yielding, when dried, 0.4 gram of 2,4,6-triamino-5-[3-(naphth-1-yl)propyl]-pyrimidine, mp 181-183°C, (dec.). The NMR spectrum was consistent with the proposed structure.

EXAMPLE 12

SYNTHESIS OF 2,4-DIAMINO-5-(2-ETHYLBUTOXY)PYRIMIDINE (Compound 56)

Step A Synthesis of 2,4-diaminopyrimidine as an intermediate

This compound was prepared in a manner analogous to that of Example 2, Step E, using 7.3 grams (0.075 mole) of 3-ethoxyacrylonitrile,

28.7 grams (0.300 mole) of guanidine hydrochloride and 55.3 grams (0.400 mole) of powdered potassium carbonate in 250 mL of N.N-dimethylformamide. The yield of 2,4-diaminopyrimidine was 7.0 grams; mp 146147°C. The NMR spectrum was consistent with the proposed structure.

The reaction was repeated on a large scale.

Step B Synthesis of 2,4-diamino-5-pyrimidinyl hydrogen sulfate as an intermediate

Under a nitrogen atmosphere, a stirred mixture of 53.7 grams (0.49 mole) of finely ground 2,4-diaminopyrimidine and 167.0 grams (0.73 mole) of ammonium persulfate was cooled to 18°C, and a solution of 144.0 grams of sodium hydroxide in 250 mL of water was added dropwise during a 3.5 hour period. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature as it stirred during about a 16 hour period. After this time the reaction mixture was cooled to 0°C, and 300 mL of 12N hydrochloric acid was added dropwise. Upon completion of addition, the reaction mixture was cooled to -5°C, and the resultant solid was collected by filtration. The solid was recrystallized from water, yielding 47.5 grams of

AP. Ο π 5 ο 3

-392,4-diamino-5-pyrimidinyl hydrogen sulfate. The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of 2,4-diamino-5-hydroxypyrimidine hydrosulfate salt 5 as an intermediate

A stirred solution of 23.0 grams (0.235 mole) of concentrated sulfuric acid and about 18 mL of water was heated to reflux, and 48.5 grams (0.235 mole) of 2,4-diamino-5-pyrimidinyl hydrogen sulfate was added in one portion. Upon completion of addition, the reaction mixture was heated at f reflux for ten minutes and then was immediately cooled in an ice-water bath. The resultant solid was collected by filtration, and the filter cake was washed with 100 mL of cold water. The solid was dried, yielding 34.0 grams of 2,4-diamino-5-hydroxypyrimidine hydrosulfate salt, mp 290°C,

5 dec. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 2,4-diamino-5-(2-ethylbutoxy)pyrimidine

A stirred solution of 0.50 gram (0.003 mole) of 2,4-diamino-520 hydroxypyrimidine hydrosulfate salt, 0.47 gram (0.007 mole) of 1-bromo-2ethylbutane and 0.95 gram (0.007 mole) of anhydrous potassium carbonate ζ in 5 mL of Ν,Ν-dimethylformamide was heated at 84-86°C for about two hours. After this time the reaction mixture was allowed to cool to ambient ( temperature as it stirred during about a 16 hour period. The reaction

5 mixture was then stirred with 50 mL of water and 50 mL of methylene chloride. The methylene chloride layer was separated and washed with 50 mL of water. The organic layer was dried with sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to a solid residue.

The residue was recrystallized from 2:1 water and methanol, yielding, when

0 dried, 0.20 gram of 2,4-diamino-5-(2- ethylbutoxy)pyrimidine. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 13

SYNTHESIS OF 2,4-DIAMINO-6-ETHYL-5-(4-CHLORO3-UNDECYLAMINOPHENYL)PYRIMIDINE (COMPOUND 74)

AP/P/ 9 3 / 0 0 5 9 5

AP 00 50 3

-40Step A Synthesis of 2,4-diamino-6-ethyl-5-(4-chloro-3-nitrophenyl)pyrimidine as an intermediate (Compound 71)

Concentrated sulfuric acid, 90 mL, and 70% nitric acid, 90 mL, were stirred together during which time the mixture warmed to about 65°C. The mixture was allowed to cool to about 50°C, and 30.0 grams (0.12 mole) of

2,4-diamino-6-ethyl-5-(4-chIorophenyi)pyrimidine (commercially available) was added portionwise during a 45 minute period, while maintaining the reaction mixture temperature at about 50-53°C. Upon completion of addition, the reaction mixture was stirred for one hour, while still maintaining the reaction mixture temperature at about 50°C. After this time, the reaction mixture was cooled and poured into ice. The resultant mixture was cooled further and, with stirring, was made basic with concentrated

5 ammonium hydroxide. A precipitate was collected by filtration, rinsed with water and dried, yielding 35.4 grams of 2,4-diamino-6-ethyl-5-(4-chloro-3nitrophenyl)pyrimidine, mp 220-223°C, dec. The NMR spectrum was consistent with the proposed structure.

0 Step B Synthesis of 2,4-diamino-6-ethyl-5-(3-amino-4-chlorophenyi)pyrimidine as an intermediate (

A heterogeneous mixture of 76.4 grams of stannous chloride ( dihydrate in 315 mL of aqueous 10M hydrochloric acid was stirred and

5 cooled to about 5-10°C. To this was added portionwise during a 15 minute period, 32.2 grams (0.11 mole) of 2,4-diamino-6-ethyl-5-(4-chloro-3nitrophenyl)pyrimidine, while maintaining the reaction mixture temperature between about 5-10°C. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature where it was stirred for about

0 16 hours. The resultant solid precipitate was collected by filtration, and stirred with 1500 mL of water. The solution was cooled in an ice bath, and the pH was adjusted to 12 with aqueous 20% sodium hydroxide. The resultant precipitate was collected by filtration and dried, yielding 26.2 grams of solid. The solid was dissolved in 350 mL of ethanol, and the

5 solution was heated at reflux. The solution was filtered hot, and the filtrate was allowed to cool. The resultant solid was collected by filtration and

AP. 00503 r

. 20 (

c

-41 dried, yielding 9.6 grams of product. The NMR spectrum was consistent with the proposed structure. The filtrate was concentrated under reduced pressure, and the residue was recrystallized from 125 ml of ethanol, yielding an additional 8.9 grams of product. The NMR spectrum was consistent with the proposed structure. The filtrate from the recrystallization was concentrated under reduced pressure, yielding another 2.3 grams of product. The NMR spectrum of this crop was also consistent with the proposed structure. The total yield of 2,4-diamino-6-ethyl-5-(3-amino-4chlorophenyl)pyrimidine was 20.8 grams.

Step C Synthesis of undecanoyl chloride as an intermediate

Under a nitrogen atmosphere, a solution of 5.0 grams (0.027 mole) of undecanoic acid in 75 mL of methylene chloride was stirred and 2.8 mi (0.032 mole) of oxalyl chloride and 5 drops of Ν,Ν-dimethylformamide were added. Upon completion of addition, the reaction mixture was stirred for about 16 hours, then it was concentrated under reduced pressure, yielding 2.2 grams of undecanoyl chloride.

Step D Synthesis of 2,4-diamino-6-ethyl-5-(4-chloro-3decylcarbonylaminophenyl)pyrimidine (Compound 85) for biological testing and as an intermediate

Under a nitrogen atmosphere, a solution of 2.5 grams (0.001 mole) of

2,4-diamino-6-ethyl-5-(3-amino-4-chlorophenyl)pyrimidine (prepared in Step B of this Example) in 75 mL of pyridine and 25 mL of tetrahydrofuran was stirred and cooled in an ice bath. To this was added dropwise during a 10 minute period, a solution of 2.2 grams (0.001 mole) of undecanoyl chloride in 50 mL of tetrahydrofuran. The reaction mixture was maintained below about 10°C throughout the addition. Upon completion of addition, the reaction mixture was stirred under a nitrogen atmosphere for about 4 days. After this time, the reaction mixture was taken up in 100 mL of water and poured into 500 mL of water. The mixture was then extracted with two 200 mL portions of ethyl acetate. The combined extracts were washed with 150 mL of aqueous 10% lithium chloride. The organic layer was dried with magnesium sulfate and filtered. The filtrate was concentrated under

AP/?/ 9 3 / 0 0 5 9 5

AP.00503

-4210

C (

reduced pressure to a residue. In an effort to remove any excess pyridine, the residue was taken up in 50 mL of toluene, and the solution was concentrated under reduced pressure to a residue. This procedure was repeated two more times. The residue was subjected to column chromatography on silica gel. Elution was accomplished with 10:1 methylene chloride and methanol. The product-containing fractions were combined and concentrated under reduced pressure, yielding 2.8 grams of

2,4-diamino-6-ethyl-5-(4-chloro-3-decylcarbonylaminophenyl)pyrimidine. The NMR spectrum was consistent with the proposed structure.

Step E Synthesis of 2,4-diamino-6-ethyl-5-(4-chloro-3-undecylaminophenyl)pyrimidine

Under a nitrogen atmosphere, a stirred solution of 40 mL of 1M borane-tetrahydrofuran complex was cooled to below 10°C, and a solution of 2.4 grams (0.006 mole) of 2,4-diamino-6-ethyl-5-(4- chloro-3decylcarbonylaminophenyl)pyrimidine in 60 mL of tetrahydrofuran was added dropwise during about a 5 minute period. The reaction mixture temperature was maintained below 10°C throughout the addition. Upon completion of addition, the reaction mixture was heated at 60°C for about 18 hours. After this time, the reaction mixture was allowed to cool to ambient temperature, where it was stirred for about 24 hours. The reaction mixture was then made acidic with aqueous 2N hydrochloric acid, after which time it was stirred for about 15 minutes. The mixture was made basic with aqueous 10% sodium hydroxide, and then it was extracted with two 150 mL portions of ethyl acetate. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a residue. The residue was subjected to column chromatography on silica gel. Elution was accomplished using 10:1 methylene chloride and methanol. The product-containing fractions were combined and concentrated under reduced pressure, yielding when dried, 0.7 gram of 2,4-diamino-6-ethyl-5-(4-chloro-3-undecylaminophenyl) pyrimidine. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 14

SYNTHESIS OF 2,4-DIAMINO-6-ETHYL-5-(3-UNDECYL35

AP. 00503

-43PHENOXY)PYRIMIDINE (COMPOUND 100)

Step A Synthesis of 3-(phenylmethoxy)phenyl bromide as an 5 intermediate

Under a nitrogen atmosphere, a solution of 50.0 grams (0.29 mole) of

3-bromophenol and 120.0 grams (0.87 mole) of potassium carbonate in 400 mL of Ν,Ν-dimethylformamide was stirred, and heated to 80°C. To this

0 was added portionwise, 36.1 mL (0.30 mole) of phenylmethyl bromide C during a 20 minute period. Upon completion of addition, the reaction mixture was stirred at 80°C during a one hour period. After this time, the reaction mixture was allowed to cool to ambient temperature, where it was diluted with 600 mL of water. The mixture was extracted with 600 mL of

5 diethyl ether. The extract was then washed with three 50 mL portions of water, and then it was concentrated under reduced pressure to a residual solid. The solid was recrystallized with 250 mL of methanol, yielding 68.3 grams of 3-(phenylmethoxy)phenyl bromide. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 1-[3-(phenylmethoxy)phenyl]undecanol as an ( intermediate ( Under a nitrogen atmosphere, a solution of 17.0 grams (0.065 mole) of 3-(phenylmethoxy)phenyl bromide in 125 mL of tetrahydrofuran was stirred and cooled to -70°C. To this was added dropwise 28.0 mL (0.071 mole) of 2.5M n-butyllithium (in hexanes) during a 15 minute period. The reaction mixture temperature was maintained below -60°C during the addition. Upon completion of addition, a solution of 11.5 grams (0.068

0 mole) of undecanaldehyde in 15 mL of tetrahydrofuran was added dropwise during a 5 minute period. Upon completion of addition, the reaction mixture was allowed to warm to 0°C during a one hour period. The reaction mixture was again cooled to -60°C, and 100 mL of water was added. The reaction mixture was allowed to warm to ambient temperature,

5 and then it was extracted with 200 mL of diethyl ether. The ether extract was washed with 50 mL of water, and then it was concentrated under

AP/P/' 93/00595

-44reduced pressure to a residue. The residue was subjected to column chromatography on silica gel. Elution was accomplished using 9:1 petroleum ether and diethyl ether, and 7:3 petroleum ether and diethyl ether. The product-containing fractions were combined and concentrated under reduced pressure, yielding 20.5 grams of 1-(3-(phenylmethoxy) phenyljundecanol. The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of 3-undecylphenol as an intermediate

A mixture of 3.0 grams (0.008 mole) of 1-(3-(phenylmethoxy) phenyljundecanol and 0.5 gram (catalyst) of 10% palladium on charcoal and 50 mL of acetic acid in 50 mL of ethanol was hydrogenated using a Parr hydrogenator. Upon the uptake of the theoretical amount of hydrogen,

5 the reaction mixture was filtered and concentrated under reduced pressure to a residual oil. NMR analysis of the oil indicated that the phenol portion of the molecule was completely unprotected, and the benzylic hydroxyl was about 70% removed. The oil was then mixed with 16.5 grams (0.047 mole) of 1-[3-(phenylmethoxy)phenyl]undecanol, 2.0 grams of 10% palladium on

0 charcoal, 10 mL of water, and 50 mL of ethanol in 130 mL of acetic acid.

The mixture was then hydrogenated as previously described, using the Parr hydrogenator. The reaction mixture was filtered and concentrated under reduced pressure to a residue. The residue was subjected to column chromatography on silica gel. Elution was accomplished using 9:1

5 petroleum ether and diethyl ether and 8:2 petroleum ether and diethyl ether.

The product-containing fractions were combined and concentrated under reduced pressure, yielding 7.7 grams of 3-undecylphenol. The NMR spectrum was consistent with the proposed structure.

0 Step D Synthesis of ethyl 2-chloro-3-oxopentanoate as an intermediate

Ethyl 3-oxopentanoate, 20.0 grams (0.175 mole), was stirred at ambient temperature, and 23.6 grams (0.175 mole) of sulfuryl chloride was added dropwise during a 20 minute period. Upon completion of addition,

5 the reaction mixture was stirred for about 16 hours. The reaction mixture

AP. 00503

-45was then distilled under reduced pressure, yielding 23.3 grams of ethyl 2chloro-3-oxopentanoate; bp 100-103°C at 21mm.

Step E Synthesis of ethyl 2-(3-undecylphenoxy)-3-oxopentanoate as an 5 intermediate

A mixture of 7.0 grams (0.028 mole) of 3-undecylphenol (prepared in Steps A-C of this Example) and 1.1 grams (0.028 mole) of 60% sodium hydride (in mineral oil) in 100 mL of toluene was stirred for about 20

0 minutes, and 4.2 grams (0.028 mole) of ethyl 2-chloro-3-oxopentanoate (prepared in Step D of this example) was added dropwise during about a 12 minute period. Upon completion of addition, the reaction mixture was heated to reflux, where it was stirred for about 6.5 hours. After this time, the reaction mixture was allowed to cool to ambient temperature, where it stood

5 for about 16 hours. The reaction mixture was then taken up in 50 mL of water and 5 mL of acetic acid. The mixture was washed with an additional 50 mL of water and then with 25 mL of an aqueous solution saturated with sodium chloride. The organic layer was dried with sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 8.9

0 grams of ethyl 2-(3-undecylphenoxy)-3-oxopentanoate. The NMR spectrum was consistent with the proposed structure.

(

Step F Synthesis of 2-amino-6-ethyl-4-hydroxy-5-(3-undecylphenoxy)pyrimidine as an intermediate

A stirred solution of 8.9 grams (0.028 mole) of ethyl 2-(3undecylphenoxy)-3-oxopentanoate and 2.5 grams (0.028 mole) of guanidine carbonate in 30 mL of ethanol was heated at reflux for about 6 hours. The reaction mixture was then concentrated under reduced

0 pressure to a residue. The residue was taken up in 50 mL of water and 5 mL of acetic acid, and the mixture was extracted with 50 mL of diethyl ether. The ether layer was extracted with 20 grams of aqueous 10% sodium hydroxide and then with 100 mL of water. The water extract was made acidic with acetic acid, and then it was extracted with 50 mL of diethyl ether.

5 The ether extract was concentrated under reduced pressure to a residue. The residue was then taken up in 25 mL of heptane, and the mixture was

AP/P/ 9 3 / 0 0 5 9 5

AP . 0 0 5 0 3 •46again concentrated under reduced pressure to a residual solid. The solid was slurried in an additional 25 mL of heptane, and then it was collected by filtration. The solid was dried under reduced pressure, yielding 1.7 grams of 2-amino-6-ethyl-4-hydroxy-5-(3-undecylphenoxy) pyrimidine. The NMR spectrum was consistent with the proposed structure.

Step G Synthesis of 2-amino-4-chIoro-6-ethyl-5-(3-undecylphenoxy) pyrimidine as an intermediate

0 A stirred solution of 1.7 grams (0.004 mole) of 2*amino-6-ethyl-4hydroxy-5-(3-undecylphenoxy)pyrimidine in 5 mL of phosphorus oxychloride was heated at reflux for about 1.5 hours. The reaction mixture was then poured into 50 grams of ice. The mixture was made basic with concentrated ammonium hydroxide. The liquid was then decanted from a tarry residue. The residue was washed with 50 mL of water, and the water was decanted from the residue. The residue, which was crude 2-amino-4chloro-6-ethyl-5-(3-undecylphenoxy)pyrimidine, was used without further purification in the next reaction.

Step H Synthesis of 2,4-diamino-6-ethyl-5-(3-undecylphenoxy) pyrimidine c

The tarry residue from Step G, which was 2-amino-4-chloro-6-ethylc 5-(3-undecylphenoxy)pyrimidine, was dissolved in 50 mL of ethanol and

5 placed in a pressure bottle. The ethanol solution was cooled in an ice bath, and an excess amount of ammonia gas was bubbled in. The pressure bottle was sealed, and the reaction mixture was heated to about 126°C, where it was stirred for about 16 hours. After this time the reaction mixture was cooled and removed from the pressure bottle. The reaction mixture

0 was concentrated under reduced pressure to a residue. The residue was taken up in 40 mL of water, 10 mL of aqueous 10% sodium hydroxide, and 50 mL of diethyl ether. The ether was removed under reduced pressure, and 40 mL of heptane was added. The heptane was also removed under reduced pressure, and 40 mL of ethyl acetate was added. The mixture was

5 shaken, and the organic layer was removed. The organic layer was washed with 25 mL of a solution saturated with sodium chloride, and then it

AP 00503

-47was dried with sodium sulfate. The mixture was filtered, and the filtrate was concentrated under reduced pressure to a residue. The residue was taken up in 15 mL of diethyl ether and cooled in dry ice. The resultant solid was collected by filtration and washed with cold diethyl ether. Upon washing the solid with cold ether, some of the solid dissolved into the filtrate. The remaining solid and 10 mL of heptane were combined with the filtrate. The combination was concentrated under reduced pressure to remove the diethyl ether. The mixture was then filtered to collect a solid. The solid was washed with heptane and dried, yielding 0.5 gram of 2,4-diamino-6-ethyl-51 0 (3-undecylphenoxy)pyrimidine; mp 97-99°C. The NMR spectrum was

C consistent with the proposed structure.

EXAMPLE 15

SYNTHESIS OF 2,4-DIAMINO-5-(3-NITRO-4-[(3,4-DICHLORO15 PHENYL)METHYLAMINO]PHENYL]-6-ETHYLPYRIMIDINE (COMPOUND 112)

Step A Synthesis of 2,4-diamino--5-(4-chloro-3-nitrophenyl)-6ethylpyrimidine as an intermediate

A stirred solution of 75 mL of 70% nitric acid and 75 mL of concentrated sulfuric acid warmed to about 50°C from the heat of solution. To this was added 25 grams (0.10 mole) of 2,4-diamino-5-(4-chlorophenyl)-6-ethylpyrimidine (commercially available) during a 10 minute

5 period. Upon completion of addition, the reaction mixture was stirred at

50°C for about 1 hour. After this time, the reaction mixture was allowed to cool to ambient temperature where it was stirred for about 18 hours. The reaction mixture was then poured into 1000 mL of ice containing 110 mL of concentrated ammonium hydroxide. The resultant solid was collected by filtration and recrystallized from aqueous ethanol, yielding 29.2 grams of

2,4-diamino-5-(4-chloro-3-nitrophenyl)-6-ethylpyrimidine. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 2,4-diamino-5-[3-nitro-4-[(3,4-dichlorophenyl)3 5 methylamino]phenyl]-6-ethylpyrimidine (Compound 112)

AP/P/ 93/00595

AP. 00503

-4810

Γ . 20 t

( c

A mixture of 2.0 grams (0.007 mole) of 2,4-diamino-5-(4-chloro-3nitrophenyl)-6-ethylpyrimidine and 15 mL of 3,4-dichlorophenylethyl-amine was stirred at 100°C for about 4 hours. After this time, the reaction mixture was poured into 250 mL of water. The water layer was decanted from an oily material, and the oily material was stirred with an additional 200 mL of water. The resultant solid was collected by filtration and dried, yielding 0.8 gram of 2,4-diamino-5-[3-nitro-4-[(3,4-dichlorophenyl)methylamino]phenyl]-6-ethylpyrimidine, mp 244-246°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 16

SYNTHESIS OF THE HYDROCHLORIDE SALT OF 2,4-DIAMlNO-5-[3(2,4,5-TRICHLOROPHENYL)PROPYL]-6-ETHYLPYRIMIDINE (COMPOUND 118)

Ethanol, 25 mL, was stirred and cooled in an ice bath and saturated with hydrogen chloride gas during a 15 minute period, while keeping the temperature of the reaction mixture below about 40°C. With continued stirring and cooling, a solution of 0.3 gram (0.0008 mole) of 2,4-diamino-5[3-(2,4,5-trichlorophenyl)propyl]-6-ethylpyrimidine (Compound 34-prepared in Example 5) in 25 mL of ethanol was added dropwise during a 2 hour period. Upon completion of addition, the reaction mixture was allowed to warm to ambient temperature, where it was allowed to stir during a 19 hour period. After this time, the reaction mixture was concentrated under reduced pressure, yielding 0.3 gram of the hydrochloride salt of 2,4diamino-5-[3-(2,4,5-trichlorophenyl)propyl]-6-ethylpyrimidine. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 17

SYNTHESIS OF 2.4-DIAMINO-5-[3-(2-TRIFLUOROMETHYLPHENYL)PROPYL]-6-METHYLPYRIMIDINE (COMPOUND 120)

Step A Synthesis of 5-(2-trifluoromethylphenyl)-4-pentynenitrile as an intermediate

AP on soj

-49Under a nitrogen atmosphere, a solution of 15.0 grams (0.055 mole) of 2-trifluoromethylphenyl iodide, 4.8 grams (0.061 mole) of 4pentynenitrile, 30.6 mL (0.22 mole) of triethylamine, 0.4 gram (0.0006 mole) of bis(triphenylphosphine)palladium(ll) chloride, and 0.1 gram (0.0006 mole) of copper(ll) iodide in 50 mL of acetonitrile was stirred at ambient temperature for 5 hours. After this time, gas chromatographic analysis (GC) of the reaction mixture indicated that the reaction was about 60% complete. The reaction mixture was warmed to 40-45°C, where it was stirred for 16 hours. After this time the reaction mixture was poured into 300 mL of water, . 1 0 and the mixture was extracted with two 100 mL portions of diethyl ether.

The combined extracts were washed with aqueous dilute hydrochloric acid and then were dried with magnesium sulfate. The mixture was filtered and concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel. Elution was

5 accomplished using 1:1 - methylene chloride and petroleum ether. The product-containing fractions were combined and concentrated under reduced pressure, yielding 11.4 grams of 5-(2-trifluoromethylphenyl)-4pentynenitrile. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 5-(2-trifluoromethylphenyl)pentanenitrile as an intermediate

A sample of 11.1 grams (0.05 mole) of 5-(2-trifluoromethylphenyl)-42 5 pentynenitrile and 0.8 grams of 10% palladium on charcoal in 150 mL of ethanol was subjected to hydrogenation using a Parr hydrogenator. The reaction did not take up the theoretical amount of hydrogen gas. The reaction mixture was filtered to remove the catalyst, and the filtrate was concentrated to a residue. The residue was subjected to column

0 chromatography on silica gel. Elution was accomplished using 2:1 petroleum ether and methylene chloride. The product-containing fractions were combined and concentrated under reduced pressure, yielding 8.7 grams of residue. The residue was hydrogenated as described above during a 3 hour period, using 0.3 gram of platinum oxide catalyst. The

5 reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to a residue. An NMR spectrum of the residue indicated

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-50that some starting material remained. The residue was again subjected to hydrogenation for 2 hours, using 0.3 gram of platinum oxide catalyst. After this time the reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to a residue. The residue was subjected to column chromatography as previously described. The product-containing fractions were combined and concentrated under reduced pressure, yielding 6.8 grams of 5-(2-trifluoromethylphenyl)pentanenitrile. The NMR spectrum was consistent with the proposed structure.

0 Step C Synthesis of 3-cyano-6-(2-trifluoromethylphenyl)-2-hexanone as an intermediate

This compound was prepared in a manner analogous to that of Step D of Example 4, using 6.5 grams (0.029 mole) of 5-(2-trifluoromethyl' 1 5 phenyljpentanenitrile, 4.2 mL (0.044 mole) of ethyl acetate, and 12.6 mL (0.032 mole) of n-butyllithium (2.5M in hexanes) in 75 mL of tetrahydrofuran. The yield of 3-cyano-6-(2-trifluoromethylphenyl)-2hexanone was 7.6 grams. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 3-cyano-2-methoxy-6-(2-trifluoromethylphenyl)-2( hexene as an intermediate ( This compound was prepared in a manner analogous to that of Step

5 D of Example 2, using 7.6 grams (0.028 mole) of 3-cyano-6-(2-trifluoromethylphenyl)-2-hexanone, 10.3 grams (0.048 mole) of Diazald, 7.0 grams (0. 125 mole) of potassium hydroxide, 12 mL of water, and 16 mL of ethanol. The yield of 3-cyano-2-methoxy-6-(2-trifluoromethyl-phenyl)-2hexene was 7.6 grams. The NMR spectrum was consistent with the

0 proposed structure.

Step E Synthesis of 2,4-diamino-5-[3-(2-trifluoromethylphenyl)propyl]-6methylpyrimidine (Compound 120)

5 Under a nitrogen atmosphere, a stirred solution of 7.6 grams (0.027 mole) of 3-cyano-2-methoxy-6-(2-trifluoromethylphenyl)-2-hexene and 12.2

AP. 00503

-51 grams (0.068 mole) of guanidine carbonate in 35 mL of N.Ndimethylacetamide was heated at about 150°C for 40 hours. After this time, the reaction mixture was poured into 300 mL of water. The resultant solid was collected by filtration, and was then slurried in 100 mL of diethyl ether.

The solid was again collected by filtration and was subjected to column chromatography on silica gel. Elution was accomplished using 10% methanol in methylene chloride. The product-containing fractions were combined and concentrated under reduced pressure, yielding 3.4 grams of

2,4-diamino-5-[3-(2-trifluoromethylphenyl)propyl]-6-methyl-pyrimidine, mp

151-158°C. The NMR spectrum was consistent with the proposed structure.

c

EXAMPLE 18

SYNTHESIS OF 2,4-DIAMINO-5-[3-(2-TRIFLUOROMETHYLPHENOXY)PROPOXY]-6-METHYLPYRIMIDINE (COMPOUND 135)

Step A Synthesis of 2.4-diamino-6-methylpyrimidine as an intermediate

A mixture of 50.0 grams (0.348 mole) of 2-amino-4-chloro-620 methylpyrimidine (commercially available) and 100 mL of aqueous 30% ammonia in 400 mL of methanol was placed in a high pressure vessel and (2 heated to 130-165°C under a pressure of 140-250 psig, where it was stirred

Γ» _λ for 13 hours. After this time, the reaction mixture was allowed to cool to * ambient temperature. The reaction vessel was then opened and the

5 reaction mixture was removed. The reaction vessel was washed with 200 mL of methanol, and the wash was combined with the reaction mixture. The combination was concentrated under reduced pressure to a residual solid. The solid was then stirred for 2 hours at ambient temperature with 100 mL of aqueous 30% ammonia. The mixture was cooled to 0°C, and the solid

0 was collected by filtration. The solid was dried, yielding 40.8 grams of 2,4diamino-6-methylpyrimidine. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 2,4-diamino-6-methyl-5-pyrimidinyl hydrogen sulfate 3 5 as an intermediate

AP/P/ 9 3 / 0 0 5 9 5

AP 00503

-52This compound was prepared in a manner analogous to that ot Step C of Example 12, using 30.0 grams (0.242 mole) of 2,4-diamino-6methylpyrimidine, 62.8 grams (0.363 mole) of ammonium persulfate and 360 mL of aqueous 5N sodium hydroxide. The product was recrystallized from water, yielding 27.3 grams of 2,4-diamino-6-methyl-5-pyrimidinyl hydrogen sulfate, mp 285°C, dec. The IR spectrum was consistent with the proposed structure.

Step C Synthesis of 2,4-diamino-5-hydroxy-6-methylpyrimidine 10 hydrosulfate salt as an intermediate

C

This compound was prepared in a manner analogous to that of Step C of Example 12, using 26.7 grams (0.121 mole) of 2,4-diamino-6-methyl-5pyrimidinyl hydrogen sulfate and 12.3 grams (0.125 mole) of concentrated

5 sulfuric acid in 50 mL of water. The yield of 2,4-diamino-5-hydroxy-6methylpyrimidine hydrosulfate salt was 27.0 grams, mp 270°C, dec.. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 2-trifluoromethylphenyl 3-bromopropyl ether as an 20 intermediate

Under a nitrogen atmosphere, a stirred mixture of 4.0 grams (0.025 mole) of 2-trifluoromethylphenol, 17.8 mL (0.178 mole) of 1,3-dibromopropane, and 9.0 grams (0.065 mole) of potassium carbonate in 120 mL of

5 acetone was heated at reflux for about 18 hours. After this time, the reaction mixture was concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel. Elution was accomplished using petroleum ether. The product containing fractions were combined and concentrated under reduced pressure, yielding 5.9

0 grams of 2-trifluoromethylphenyl 3-bromopropyl ether. The NMR spectrum was consistent with the proposed structure.

Step E Synthesis of 2,4-diamino-5-[3-(2-trifluoromethylphenoxy)propoxy]-6-methylpyrimidine (Compound 135)

-53This compound was prepared in a manner analogous to that of Step D of Example 12, using 2.4 grams (0.010 mole) of 2,4-diamino-5-hydroxy-6methylpyrimidine hydrosulfate salt (prepared in Steps A-C of this Example), 3.0 grams (0.011 mole) of 2-trifluoromethylphenyl 3-bromopropyl ether, and

8.3 grams (0.060 mole) of finely ground potassium carbonate in 50 mL of

Ν,Ν-dimethylformamide. The product was recrystallized from methanol and water, yielding 0.8 gram of 2,4-diamino-5-[3-(2trifluoromethylphenoxy)propoxyj-6-methylpyrimidine, mp 132-134°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 19

SYNTHESIS OF 2,4-DIAMINO-5-(ADAMANT-1-YL)-6-METHYLPYRIMIDINE (COMPOUND 110)

5 Step A Synthesis of ethyl 2-(adamant-1 -yl)-3-oxobutanoate as an intermediate

A stirred solution of 5.0 grams (0.033 mole) of adamantan-1-ol and

4.7 grams (0.036 mole) of ethyl acetoacetate in 60 mL of pentane was cooled to 7°C, and 5.1 grams (0.036 mole) of boron trifluoride etherate was added during a 2 minute period. An additional 3.8 mL of boron trifluoride

C etherate was then added, also during a 2 minute period. Upon completion

- of addition, the reaction mixture was allowed to warm to ambient temperature, where it was stirred for about 16 hours. After this time, the

5 reaction mixture was again cooled to 7°C, and 24 grams of aqueous 50% potassium hydroxide was added. The reaction mixture was then made acidic with 12 mL of acetic acid. The reaction mixture was poured into a separatory funnel with 50 mL of water and 130 mL of diethyl ether. The mixture was shaken and the layers were separated. The water layer was

0 washed with two portions of 30 mL each of toluene. The toluene washes were combined with the diethyl ether layer, and 100 mL of water was added to the combination. The mixture was filtered and the layers were separated. The organic layer was dried with sodium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 6.4 grams of ethyl 23 5 (adamant-1-yl)-3-oxobutanoate. The NMR spectrum was consistent with the proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

-54Step Β Synthesis of 2-amino-4-hydroxy-5-(adamant-1-yl)-6-methylpyrimidine as an intermediate

A stirred mixture of 5.4 grams (0.02 mole) of ethyl 2-(adamant-1-yl)-3oxobutanoate, 2.0 grams (0.02 mole) of guanidine hydrochloride and sodium ethoxide [prepared by adding 1.6 grams (0.04 mole) of 60% sodium hydride (in mineral oil) to 30 mL of ethanol] in 67.5 mL of ethanol was heated at reflux for about 20 hours. After this time, the reaction mixture was concentrated under reduced pressure to a residue. The residue was taken up in 50 mL of water and 5 mL of acetic acid. The mixture was filtered, and the filter cake was washed with two 50 mL portions of water. The filter cake was triturated in hot ethanol, and the mixture was allowed to cool. A solid was collected by filtration, yielding when dried, 2.6 grams of 2-amino-4hydroxy-5-(adamant-1-yl)-6-methyl-pyrimidine, mp 280-287°C. The NMR spectrum was consistent with the proposed structure.

Step C Synthesis of 2-amino-4-chloro-5-(adamant-1-yl)-6methylpyrimidine hydrochloride as an intermediate

A stirred solution of 2.4 grams (0.009 mole) of 2-amino-4-hydroxy-5(adamant-1-yl)-6-methylpyrimidine and 2.4 grams (0.012 mole) of phosphorus pentachloride in 24 mL of phosphorus oxychloride was heated at reflux for about 90 minutes. After this time, the reaction mixture was poured into 600 mL of ice. The resultant solid was collected by filtration and washed with water, yielding, when dried, about 1.8 grams of 2-amino-4chloro 5- (adamant-1 -yl)-6-methylpyri midi ne hydrochloride. The NMR spectrum was consistent with the proposed structure.

Step D Synthesis of 2,4-diamino-5-(adamant-1-yl)-6-methylpyrimidine (Compound 110)

This compound was prepared in a manner analogous to that of Step H of Example 12, using 0.92 gram (0.003 mole) of 2-amino-4-chloro-5(adamant-1-yl)-6-methylpyrimidine hydrochloride and 50 mL of aqueous 30% ammonium hydroxide. The yield of 2,4-diamino-5-(adamant-1-yl)-6AP 00503

-55methylpyrimidine was 0.13 gram, mp 254-258°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 20

SYNTHESIS OF 2,4-DIAMINO-5-[4-(2,4,5-TRICHLOROPHENYL)BUTYL]-6-ETHYLPYRIMIDINE (COMPOUND 38)

This compound was prepared in a manner analogous to that of 1 0 Example 5, using 7.0 grams (0.020 mole) of 1-(2,4,5-trichlorophenyl)-5cyano-6-methoxy-5-octene, 7.7 grams (0.080 mole) of guanidine hydrochloride, and 11.0 grams (0.080 mole) of potassium carbonate in 40 mL of N.N-dimethylformamide. The yield of 2,4-diamino-5-[4-(2,4,5trichlorophenyl)butyl]-6-ethylpyrimidine was 1.5 grams, mp 158-163°C. The

5 NMR spectrum was consistent with the proposed structure.

EXAMPLE 21

SYNTHESIS OF 2,4-DIAMINO-5-(2-ETHYLBUTOXY)PYRIMIDINE (COMPOUND 56)

The compound of this example was prepared in a manner analogous ( to that of Example 12, using 0.50 gram (0.003 mole) of 2,4-diamino-5_r hydroxypyrimidine hydrosulfate salt, 0.47 gram (0.007 mole) of 1-bromo-2ethylbutane, and 0.95 gram (0.007 mole) of anhydrous potassium

5 carbonate in 5 mL of N.N-dimethylformamide. The yield of 2,4-diamino-5(2-ethylbutoxy)pyrimidine was 0.21 gram, mp 173-174°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 22

0 SYNTHESIS OF 2,4-DIAMINO-5-[4-(4CHLOROPHENYL)BUTYL]PYRIMIDINE (COMPOUND 67)

This compound was prepared in a manner analogous to that of 3 5 Example 3, using 2.7 grams (0.010 mole) of 1 -ethoxy-2-cyano-6-(4chlorophenyl)-1-hexene, 3.9 grams (0.041 mole) of guanidine

AP/P/ 9 3 / 0 0 5 9 5

-56Al· hydrochloride, and 5.7 grams (0.041 mole) of anhydrous potassium carbonate in 5 mL of N.N-dimethylformamide. The yield of 2,4-diamino-5(4-(4-chlorophenyl)butyl]pyrimidine was 0.9 gram, mp 159-162°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 23

SYNTHESIS OF 2,4-DIAMINO-5-[3-(2,4,5-TRICHLOROPHENYL)PROPYL]-6-METHYLPYRIMIDINE (COMPOUND 117)

This compound was prepared in a manner analogous to that of Example 5, using 2.7 grams (0.008 mole) of 1 -(2,4,5-trichlorophenyl)-4cyano-5-methoxy-4-hexene, 2.1 grams (0.022 mole) of guanidine hydrochloride, and 3.0 grams (0.022 mole) of anhydrous potassium

5 carbonate in 40 mL of N.N-dimethylformamide. The yield of 2,4-diamino-5[3-(2,4,5-trichlorophenyl)propyl]-6-methylpyrimidine was 0.5 gram, mp 215218°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 24

0 SYNTHESIS OF 2,4-DIAMINO-5-[3-(2-METHYLPHENYL)PROPYL]-6-METHYLPYRIMIDINE C (COMPOUND 123) r

^v This compound was prepared in a manner analogous to that of

5 Steps A-F of Example 5, and Step E of Example 17, using 3.2 grams (0.014 mole) of 1-(2-methylphenyl)-4-cyano-5-methoxy-4-hexene, and 6.3 grams (0.035 mole) of guanidine carbonate in 75 mL of N.N-dimethylacetamide. The yield of 2,4-diamino-5-[3-(2-methylphenyl)propyl]-6-methylpyrimidine was 0.5 gram, mp 213-215°C. The NMR spectrum was consistent with the

0 proposed structure.

EXAMPLE 25

SYNTHESIS OF 2,4-DIAMINO-5-[3-(4-METHYLPHENYL)PROPYL]-6-METHYLPYRIMIDINE (COMPOUND 125)

-57This compound was prepared in a manner analogous to that of Steps A-F of Example 5, and Step E of Example 17, using 1.4 grams (0.006 mole) of 1 (4-methylphenyl)-4-cyano-5-methoxy-4-hexene, and 2.7 grams (0.015 mole) of guanidine carbonate in 20 mL of Ν,Ν-dimethylacetamide. The yield of 2,4-diamino-5-(3-(4-methylphenyl)propyl]-6-methylpyrimidine was 0.1 gram, mp 210-213°C. The NMR spectrum was consistent with the proposed structure.

0 EXAMPLE 26

SYNTHESIS OF 2,4-DIAMINO-5-[3-(2,4,5-TRIMETHYLPHENYL)PROPYL]-6-METHYLPYRIMIDINE (COMPOUND 128)

5 This compound was prepared in a manner analogous to that of

Steps A-F of Example 5, and Step E of Example 17, using 4.4 grams (0.017 mole) of 1-(2,4,5-trimethylphenyl)-4-cyano-5-methoxy-4-hexene, and 7.1 grams (0.042 mole) of guanidine carbonate in 25 mL of N.Ndimethylacetamide. The yield of 2,4-diamino-5-[3-(2,4,5-trimethyl20 phenyl)propyl]-6-methylpyrimidine was 2.9 grams, mp 188-190°C. The NMR spectrum was consistent with the proposed structure.

c

EXAMPLE 27 c' SYNTHESIS OF 2,4-DIAMINO-5-(2-ETHYLBUTOXY)-62 5 METHYLPYRIMIDINE (COMPOUND 132)

This compound was prepared in a manner analogous to that of Examples 12 and 18 , using 2.4 grams (0.010 mole) of 2,4-diamino-63 0 methyl-5-hydroxypyrimidine hydrosulfate salt, 2.8 mL (0.020 mole) of 1bromo-2-ethylbutane, and 8.3 grams (0.060 mole) of anhydrous potassium carbonate in 5 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5(2-ethylbutoxy)-6-methylpyrimidine was 0.6 gram, mp 142-143°C. The NMR spectrum was consistent with the proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

AP. 0 η 5 0 3

-58EXAMPLE 28

SYNTHESIS OF 2,4-DIAMINO-5-(3-PHENYLPROPOXY)-6-METHYLPYRIMIDINE (COMPOUND 134)

This compound was prepared in a manner analogous to that of Examples 12 and 18 , using 2.1 grams (0.009 mole) of 2,4-diamino-5hydroxy-6-methylpyrimidine hydrosulfate salt, 2.7 mL (0.018 mole) of 3phenylpropyl bromide, and 7.6 grams (0.055 mole) of anhydrous potassium carbonate in 40 mL of Ν,Ν-dimethylformamide. The yield of 2,4-diamino-5(3-phenylpropoxy)-6-methylpyrimidine was 0.7 gram, mp 160.5-161.5°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 29

SYNTHESIS OF 2,4-DIAMINO-5-[3-(3-TRIFLUOROMETHYLPHENYL)BUTYL]-6-ETHYLPYRIMIDINE (COMPOUND 137)

Step A Synthesis of 5-hydroxy-5-(3-trifluoromethylphenyl)hexanenitrile as an intermediate

A mixture of 7.0 mL (0.05 mole) of 3-trifluoromethylphenyl bromide,

1.2 grams (0.05 mole) of magnesium and a crystal of iodine in about 70 mL of tetrahydrofuran was warmed to 35°C to initiate the formation of the Grignard reagent. Upon formation of the Grignard reagent, the reaction mixture was cooled to ambient temperature and, with stirring, a solution of

5.8 mL (0.05 mole) of 5-oxohexanenitrile in 10 mL of tetrahydrofuran was added dropwise. The reaction mixture temperature increased to about 35°C, at which time the rate of addition was slowed to keep the reaction mixture temperature at about 35-40°C. The complete addition required about 15 minutes. Upon completion of the addition, the reaction mixture was allowed to warm to ambient temperature, where it was stirred for 2 hours. After this time, the reaction mixture was poured into 200 mL of water and was acidified with aqueous 2N hydrochloric acid. The mixture was then extracted with two 150 mL portions of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was

AP ¢0503

-59concentrated under reduced pressure to a residual oil. The oil was subjected to column chromatography on silica gel. Elution was accomplished using methylene chloride. The product-containing fractions were combined and concentrated under reduced pressure, yielding 6.3 grams of 5-hydroxy-5-(3-trifluoromethylphenyl)hexanenitrile. The NMR spectrum was consistent with the proposed structure.

Step B Synthesis of 5-(3-trifluoromethylphenyl)hexanenitrile as an intermediate

Under a nitrogen atmosphere, a solution of 4.4 grams (0.017 mole) of 5-hydroxy-5-(3-trifluoromethylphenyl)hexanenitrile, 13 mL (0.102 mole) of chlorotrimethylsilane, 15.4 grams (0.102 mole) of sodium iodide, and 6.0 mL (0.153 mole) of acetonitrile in 30 mL of hexane was stirred for about 30

5 minutes. After this time, gas chromatographic analysis of the reaction mixture indicated that the reaction was complete. The reaction mixture was then stirred with 100 mL of water, and then it was extracted with 100 mL of diethyl ether. The combined extracts were dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to a

0 residue. The residue was subjected to column chromatography on silica gel. Elution was accomplished using methylene chloride. The methylene ( chloride eluate was dried with magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure, yielding 1.9 grams of 5-(3( trifluoromethylphenyl)hexanenitrile. The NMR spectrum was consistent with

5 the proposed structure.

Step C Synthesis of 4-cyano-7-(3-trifluoromethylphenyl)-3-octanone as an intermediate

0 This compound was prepared in a manner analogous to that of Step

E of Example 4, using 1.9 grams (0.008 mole) of 5-(3-trifluoromethylphenyl)hexanenitrile, 1.0 mL (0.009 mole) of ethyl propionate , and 3.6 mL (0.009 mole) of n-butyllithium (2.5M in hexanes) in 75 mL of tetrahydrofuran. The yield of 4-cyano-7-(3-trifluoromethylphenyl)-33 5 octanone was about 2.1 grams. The NMR spectrum was consistent with the proposed structure.

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-60Step D Synthesis of 4-cyano-3-methoxy-7-(3-trifluoromethylphenyl)-3octene as an intermediate

This compound was prepared in a manner analogous to that of Step

E of Example 2, using 2.1 grams (0.007 mole) of 4-cyano-7-(3-trifluoromethylphenyl)-3-octanone, 2.6 grams (0.012 mole) of Diazald, 4.0 grams of potassium hydroxide, and 7.0 mL of water in 12 mL of ethanol. The yield of

4-cyano-3-methoxy-7-(3-trifluoromethylphenyl)-3-octene was 2.1 grams.

0 The NMR spectrum was consistent with the proposed structure.

Step E Synthesis of 2,4-diamino-5-I3-(3-trifluoromethylphenyl)butylJ-6ethylpyrimidine (Compound 137)

5 This compound was prepared in a manner analogous to that of Step

E of Example 17, using 2.1 grams (0.007 mole) of 4-cyano-3-methoxy-7-(3trifluoromethylphenyl)-3-octene, and 3.1 grams (0.018 mole) of guanidine carbonate in 25 mL of Ν,Ν-dimethylacetamide. The yield of 2,4-diamino-5[3-(3-trifluoromethylphenyl)butyl]-6-ethylpyrimidine was 0.7 gram, mp 932 0 96 °C. The NMR spectrum was consistent with the proposed structure.

( EXAMPLE 30

SYNTHESIS OF 2,4-DIAMINO-5-I3-(4-TRIFLUORO METHYLPHENYL)BUTYL]-6-ETHYLPYRIMIDINE

5 (COMPOUND 137)

This compound was prepared in a manner analogous to that of Example 29, using 7.3 grams (0.023 mole) of 4-cyano-3-methoxy-7-(4trifluoromethylphenyl)-3-octene, and 10.5 grams (0.058 mole) of guanidine

0 carbonate in 30 mL of Ν,Ν-dimethylacetamide. The yield of 2,4-diamino-5(3-(4-trifluoromethylphenyl)butyl]-6-ethylpyrimidine was 2.4 grams, mp 4855°C. The NMR spectrum was consistent with the proposed structure.

ΑΡ π π 5 Ο 3

-61 EXAMPLE 31

SYNTHESIS OF 2,4-DIAMINO-5-[3-(2,4-DICHLOROPHENYL)BUTYL]-6-METHYLPYRIMIDINE (COMPOUND 139)

This compound was prepared in a manner analogous to that of Example 29, using 1.9 grams (0.006 mole) of 3-cyano-2-methoxy-6-(2,4dichlorophenyl)-2-heptene and 2.8 grams (0.015 mole) of guanidine carbonate in 30 mL of Ν,Ν-dimethylacetamide. The yield of 2,4-diamino-51 0 [3-(2,4-dichlorophenyl)butyl]-6-methylpyrimidine was 0.6 gram, mp 164C 168°C. The NMR spectrum was consistent with the proposed structure.

EXAMPLE 32

SYNTHESIS OF 2,4-DIAMINO-5-[3-(4-CHLORO1 5 PHENYL)BUTYL]-6-METHYLPYRIMIDINE (COMPOUND 140)

This compound was prepared in a manner analogous to that of Example 29, using 5.3 grams (0.02 mole) of 3-cyano-2-methoxy-6-(420 dichlorophenyl)-2-heptene and 9.2 grams (0.05 mole) of guanidine carbonate in 30 mL of Ν,Ν-dimethylacetamide. The yield of 2,4-diamino-5( [3-(4-dichlorophenyl)butyl]-6-methylpyrimidine was 1.7 grams, mp 125128°C. The NMR spectrum was consistent with the proposed structure.

5 EXAMPLE 33

SYNTHESIS OF 2,4-DIAMINO-5-[3-(4,4-DICHLOROPHENYL)PROPYL]-6-METHYLPYRIMIDINE (COMPOUND 136)

0 The intermediates to Compound 136 were prepared in a manner analogous to that of Steps A - F of Example 5. Compound 136 was prepared as taught in Step D of Example 17, using 7.8 grams (0.028 mole) of 3-cyano-2-methoxy-6-(2,4-dichlorophenyl)-2-hexene and 12.4 grams (0.069 mole) of guanidine carbonate in 30 mL of N.N-dimethylacetamide.

5 The yield of 2,4-diamino-5-[3-(2,4-dichlorophenyl)propyl]-6-methylAP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-62pyrimidine was 1.0 gram; mp 181-185°C. The NMR spectrum was consistent with the proposed structure.

Appended TABLE 1 lists 141 species of pyrimidines and salts thereof 5 falling within Formula I (supra) of this invention, the preparation of certain of which species are illustrated in accordance with foregoing Examples 1 33.

<

C

AP. 0 0 5 0 3

-63TABLE 1

5-SUBSTITUTED-2.4-DIAMINOPYRIMIDINES

	NH2 ΛΪ RWN^N^R’
where m and p are 0, and R2, R3 are hydrogen
Cmpd No.	n	R	R1
1	0	H	-ch3
2	0	H	n-pentyl
3	5	ch3	H
4	11	-ch3	H
5	1	-ch3	phenyl
6	2	cyclohexyl	H
7	1	H	n-propyl
8	1	ch3	4-phenylbutyl
9	2	ch3	2-phenylethyl
10	3	ch3	phenylmethyl
11	3	ch3	5-phenylpentyl
12	4	ch3	3-phenylpropyl
13	1	cyclohexyl	ethyl
14	3	cyclohexyl	ethyl

AP/P/ 9 3 / 0 0 5 9 5

ΑΡ ο ο 5 0 J

-64Table 1 (cont'd) where m and p are 0, R², R² are hydrogen, and R is v

γ

	Cmpd. No.n	R1	V	W	X	Y	Z
c	15	0	H	H	H	H	H	H
	16	1	H	H	H	H	H	H
	17	1	H	H	H	-OCH3	H	H
	18	1	H	H	F	H	F	H
	19	1	H	H	Cl	Cl	H	Cl
	20	1	H	H	-OCH3	-OCH3	-OCH3	H
	21	1	-c2h5	H	H	H	H	H
n	22	1	-c2h5	H	0	H	H	H
	23	1	-C2H5	Cl	H	Cl	Cl	H
c	24	1	n-bulyl	H	H	H	H	H
	25	2	H	H	H	H	H	H
	26	2	H	H	H	Cl	H	H
	27	2	H	H	0	H	H	H
	28	2	H	Cl	H	Cl	Cl	H
	29	2	-ch3	H	H	H	H	H
	30	2	-c2h5	H	H	-OCH3	H	H
	31	3	H	H	H	H	H	H

AP. 00503

Table 1 (cont'd)	z
Cmpd No. n	R1	V	W	X	Y
32	3	H	H	H	-OCH3	H	H
33	3	-c2h5	H	0	H	H	H
34	3	-c2h5	Cl	H	Cl	Cl	H
35	3	n-propyl	H	H	H	H	H
36	3	n-pentyl	H	H	H	H	H
37	4	H	H	H	H	H	H
38	4	-c2h5	Cl	H	Cl	Cl	H
39	4	-C2H5	Cl	H	Cl	Cl	H

hydrochloride salt

40	4	0	H	H	H	H	H
41	5	H	H	H	H	H	H
42	5	2-phenylethyl	H	H	H	H	H
43	5	4-phenylbutyl	H	H	H	H	H
44	6	H	H	H	H	H	H

AP/P/ 9 3 / 0 0 5 9 5

AP. ί) Ο 5 Ο 3

-66Table 1 (cont’d) where m and p are 0 and R is

	Cmpd No. n	R1	R2	R3
	45	1	H	H	H
r	46	2	-C2H5	H	H
	47	3	H	H	H
	48	3	-nh2	H	H
	49	3	H	-ch3	H
	50	3	H	-c2h5	H
	51	3	H	-CH3	-CH3
	52	3	H	-ch2ch2och2ch2-
(	53	4	H	H	H
(	54	5	H	H	H

55 6	Η Η H
where m is 1, p is 0 and R1, R2, R3 are hydrogen
Cmpd No. n	R
56 1	-CH(C2H5)2
57 11	-ch3
58 4	-CN

AP. Ο Ο 5 Ο 3

-67Table 1 (cont'd) where m is 1, p is 0, R¹, R², R³ are hydrogen, and R is

γ

Cmpd No.	n	V	W	X	Y	Z
59	1	H	F	H	F	H
60	1	Cl	H	Cl	Br	H
61	5	H	H	-CO2C2H5	H	H

where m and p are 0; R¹, R² and R³ are hydrogen

Cmpd No.	n	R
62	7	-ch3
63	7	-ch3 Hydrochloride
64	1	-Si(CH3)3
65	3	-Si(CH3)3
66	3

AP/P/ 93/00595

AP. 00503

-68Table 1 (cont'd) where m and p are 0, R¹, R² and R³ are hydrogen, and R is v

where V, W, Y, and Z are hydrogen

Cmpd No.	n	X
67	4	-Cl
68	4	-Cl Hydrochloride
69	5	-OCH3
70	7	-OCH3

where m, n and p are 0, R² and R³ are hydrogen, R¹ is ethyl, and R is

where V, Y, and Z are hydrogen; and X is chloro.

Cmpd No. W	R5
71	-no2	—
72	-NHR5	-θδΗ11
73	-NHR5	-C5HH -3HCI
74	-NHR5	-C11H23

Table 1 (cont’d) Cmpd No.	W	R5
75	-NHR5
76	-NHR5	~“0 · 3HCI
77	-NHR5
78	-NHR5
79	-NHR5	C2H4O—
80	-NHR5	<h>_O
81	-NHR5	. 2HCI

Cmpd No.	W	R4
82	-NHC(O)R4	-C4H9
83	-NHC(O)R4	-C(CH3)3
84	-NHC(O)R4	-C9H19
85	-NHC(O)R4	-C10H21
86	-NHC(O)R4	-C3F7
87	-NHC(O)R4	-O
88	-NHC(O)R4	-c^-Q-a
89	-NHC(O)R4	CHjO—SOjC,H7

ΑΡ/Ρ/ 9 3 / 0 0 5 9 5

00703

-70Table 1 (cont'd) where m is 0, p is 1, n is 3, R¹, R² and R³ are hydrogen Cmpd No. R

-C4H9 xo where m is 0, p is 1, R¹, R², and R³ are hydrogen, and R is v

where V, W, Y, and Z are hydrogen

Cmpd No.	n	X
	92	3	-SC3H7
	93	3	-SO2C3H7
(	94	3	-SO2C3H7 Hydrochloride
	95	5	-SC3H7
	96	5	-SO2C3H7

Cmpd No. m	n	p R R1	R2	R3
97 1	1	0 -CH(C2H5)2 H	H	H

AP. 00503

-71 Table 1 (cont'd) where m is 1, p is 0, and R^ and R3 are hydrogen, and R is v

γ where Z is hydrogen.

Cmpd No.	n	R1	V	W	X
98	1	H	H	H hydrochloride	-c2h5
99	1	H	Cl	H	Cl
100	0	Ό2Η5	H	-C11H23	H
101	0	-C2H5	H	-CH(C2H5)2	H
102	0	-C2H5	H	-OC4H9	H
103	0	-C2H5	H	-CH2Si(CH3)3	H
104	5	H	H	H	-CO2C2H5
105	0	-C2H5	H		H
106	0	-C2H5	H	C4H,—°°Η3 H
107	0	-C2H5	H	-CH(OH)—	H
108	0	-C2H5	H	C2H2O—SO2C,H7 H
109	0	-C2H5	H		H

AP/P/ 9 3 / 0 0 5 9 5

AP. 0 0 5 0 3

-72Table 1 (cont’d) where m, p are 0, R² and R³ are hydrogen; Cmpd No. n

110

111

R¹

-CH3

H adamantyl cyclohexyl where m, p are 0, R², R³ and Z are hydrogen, and R is v

npd No. 112	n 0	R1 -C2H5	V H	W -NO2	X Cl nhch2-^ Cl	Y H
113	0	-C2H5	H	-NO2	-nhch2	H
					och3
114	3	-CH3	H	H	Cl	H
115	3	H	Cl	H	Cl	C
116	3	H	Cl	H	Cl	Cl
			HCI salt
117	3	-CH3	Cl	H	Cl	Cl
118	3	-C2H5	Cl	H	Cl	Cl
			HCI salt
119	5	-C2H5	Cl	H	Cl	Cl
120	3	-CH3	-cf3	H	H	H
121	3	-CH3	H	-CF3	H	H
122	3	-CH3	H	H	-cf3	H

AP.00503

123	3	-CH3	-CH3	H	H	H
Table 1 (cont’d)
Cmpd No.	n	R1	V	W	X	Y
124	3	-CH3	H	-CH3	H	H
125	3	-CH3	H	H	-CH3	H
126	3	-CH3	H	-CH3	-CH3	H
127	3	-CH3	H	-ch3	H	-CH3
128	3	-CH3	-CH3	H	-CH3	-CH3
129	3	-CH3	H	-OC2H5	H	H
130	3	-ch3	H	H	-OC2H5	H
131	3	-CH3	H	H	-OC3H7	H

where m is 1, p is 0, R² and R³ are hydrogen

Cmpd No.	n	R	R1
132	1	-CH(C2H5)2	-CH3
133	1	cyclopentyl	H
where n is 3, R1	is methyl, R2 and R3 are hydrogen, and R is Y

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-74Table 1 (cont’d)

Cmpd No.	m	P	V	w	X	Y	Z
134	1	0	H	H	H	H	H
135	1	1	-cf3	H	H	H	H
136	0	0	Cl	H	Cl	H	H

where m and p are 0, n is 2, R² and R³ are hydrogen, and R is

		Ti*r	^v1
	Y1X	X1	Sw1		(III)
Cmpd No.	R1	q	V1	wi	X1	Y1	Z1
137	-C2H5	1	H	-cf3	H	H	H
138	-C2H5	1	H	H	-cf3	H	H
139	-CH3	1	Cl	H	Cl	H	H
140	-ch3	1	H	H	Cl	H	H
141	-ch3	1	H	Cl	H	Cl	H

AP . 00503

-74aTablel (cont'd)

5-SUBSTITUTED 2,4-DIAMINOPYRIMIDINES

(O)m-(CH2)n-(O)_p-R

R where m, p are 0; R¹ is methyl, R²· R3, RG, and R⁷ are hydrogen; and R is

Cmpd. No	n	2	y1	w1	X1	Ϊ1	Z1
142	2	1	H	Cl	Cl	H	H
143	2	1	H	H	F	H	H
144	3	1	H	H	Cl	H	H
145	3	1	H	H	-OCH3	H	H

AP/P/ 9 3 / 0 0 5 9 5

AP 00503

-74bTable 1 (cont'd) where m, p are 0; R¹ is methyl, R²· R³, R⁶, and R⁷ are hydrogen; and R is

Cmpd No	n	V	w	X	Y	z
146	3	H	H	F	H	H
147	3	H	Cl	Cl	H	H
148	3	H	H	-C2F5	H	H
149	3	H	H	n-C4Fg	H
150	3	Cl	H	-cf3	H	H
151	3	H	H	-OCF3	H	H
152	3	H	H	-OCH3	H	H
153	3	H	H	-SCH3	H	H
154	3	H	H	-SO2CH3	H	H
155	3	H	H	Λ,	H	H
156	4	Cl	H	Cl	H	H
157	4	Cl	H	-OCH3	H	H
158	4	H	H	-OCH3	H	H

AP. Ο Π 5 Ο 3

-74cTable 1 (cont’d) where mis 0; p is 1; R¹ is methyl, R²· R³, R⁶, and R⁷ are hydrogen; and R is

Cmpd No η V

159 4 -CF3

AP/P/ 9 3 / 0 0 5 9 5

AP. C Ο 5 Ο 3

-75TABLE-Ba

MELTING POINT / EMPIRICAL FORMULA

Compound Number	Melting Point (°C)	Empirical Formula
1	208-210	c5h8n
2		C9H16N4
3	120-125	C10H18N4
4	123-125	C16H30N4
5	186-188	C12H14N4
6	177-179	C12H20N4
7	164-165	C8H14N4
8	340-343, dec.	C13H10CI2N6
9	127-128	C15H20N4
10	156-161	C15H20N4
11	96-98	C19H28N4
12	93-94	C18H26N4
13	172-176	C13H22N4
14	143-148	C15H26N4
15	162-164	C10H10N4
16	195-196	C11H12N4
17	195-200	C12H14N4O
18	243-245	C11H10F2N4
19	246-248	C11H9CI3N4
20		C14H18N4O3
21	157-160	C13H16N4
22	108-115	C19H20N4
23	176-180, dec.	C13H13CI3N4
24	159-161	C15H20N4
25	143-145	C12H14N4
26	200-203	C12H13CIN4
27	138-141	C18H18N4
28	218-222	C12H11CI3N4
29	146-149	Cl3H16N4
30	143-147	C15H20N4O
31	140-142	C13H16N4
32	151-154	C14H18N4O
33	128-133	C21H24N4
34	168-182	C15H17CI3N4
35	137-139	C16H22N4
36	130-132	C18H26N4

AP. 00503

-76Table 1-a

MELTING POINT / EMPIRICAL FORMULA

Compound Number	Melting Point (°C)	Empirical Formula
37	126-127	C14H18N4
38	158-163	C16H19CI3N4
39	255	ΟιβΗ20θΐ4Ν4
40	149-153	C20H22N4
41	111-113	C15H20N4
42	120-122	C23H28N4
43	75-78	C25H32N4
44	99-102	C16H22N4
45	226-231	C15H14N4
46	181-183	C18H20N4
47	179-181	Ci7Hi8N4
48	181-183, dec.	C17H19N5
49	92-95	C18H20N4
50	—	C19H22N4
51	105-110	C19H22N4
52	103-107	C21H24N4O
53	152-155	C18H20N4
54	128-133	C19H22N4
55	98-102	C20H24N4
56	173-174	C10H18N4O
57	103-107	C16H30N4O
58	195-198	C9H13N5O
59	173-175	C11H10F2N4O
60	201-202	CnH9BrCl2N4O
61	132-134	C18H24N4O3
62	115-119	C12H22N4
63	226-230	C12H23CIN4
64	—	C8H16N4S1
65	158-161	C10H20N4S1
66	200-202	C11H19N5O
67	159-162	C14H17CIN4
68	243-245	C14H18CI2N4
69	128-132	C16H22N4O
70	124-126	C18H26N4O
71	223-226	C12H12CIN5O2
72	134-136	C17H24CIN5

io σ>

LO

O o

ro

CJ>

tc

CL «X

AP. 00503

-77Iabifi_ha

MELTING POINT / EMPIRICAL FORMULA

Compound Number	Melting Point (°C)	Empirical Formula
73	292-294, dec.	C17H27CI4N5
74	102-112	C23H36CIN5
75	178-182	C17H22CN5
76	327-329, dec.	C17H25CI4N5
77	84-92	C19H19CI2N5
78	61-70	C22H25CI2N5
79	92-96	C23H28CIN5O3S
80	177-180	CieH-igClNe
81	182-192	C18H21CI3N6
82	104-105	C17H22CIN5O
83	260-261	C17H22CIN5O
84	77-85	C22H32CIN5O
85	55-66	C23H34CIN5O
86	136-140	C16H13CIF7N5O
87	217-225	C19H17CI2N5O
88	134-137	C22H23CI2N5O
89	104-120	C23H26CIN5O4S
90	77-80	C11H20N4O
91	117-124	C17H28N4O
92	89-93	C16H22N4OS
93	165-172	C16H22N4O3S
94	218	C16H23CIN4O3S
95	107-111	C18H26N4OS
96	138-148	C18H26N4O3S
97	173-174	C10H18N4O
98	198, dec.	C13H17CIN4O
99	201-202	Ci 1 HgBrCte^O
100	97-99	C23H36N4O
101	GUM	C17H24N4O
102	130-133	C16H22N4O2
103	84-96	Ci6H24N4OSi
104	132-134	C18H24N4O3
105	GUM	C19H19CIN4O
106	GUM	C23H28N4O2
107	250	C19H20N4O2
108	GUM	C23H28N4O4S

AP. 00503

-78Table.-1-a

MELTING POINT / EMPIRICAL FORMULA

Compound Number	Melting Point (°C)	Empirical Formula
109	GUM	C22H30N4O2
110	254-258	C15H22N4
111	148-153	C14H24N4
112	244-246	C19H18CI2N6O2
113	246-247	C20H22N6O3
114	148-149	C14H17CIN4
115	192-196	C13H13CI3N4
116	—	C13H14CI4N4
117	215-218	C14H15CI3N4
118	—	C15H18CI4N4
119	126-131	C17H21CI3N4
120	151-158	C15H17F3N4
121		C15H17F3N4
122	185-186	C15H17F3N4
123	213-215	C15H20N4
124	—	C15H20N4
125	210-213	C15H20N4
126	169-172	C16H22N4
127	173-176	C16H22N4
128	188-190	C17H24N4
129	—	C16H22N4O
130	113-115	C16H22N4O
131	109-111	C17H24N4O
132	142-143	C11H20N4O
133	139-144	C10H16N4O
134	160.5-161.5	C14H18N4O
135	132-134	C15H17F3N4O2
136	181-185	C14H16CI2N4
137	93-96	C17H21F3N4
138	48-55	C17H21F3N4
139	164-168	C15H18CI2N4
140	125-128	C15H19CIN4
141	—	C15H18CI2N4

AP/P/ 33/00595

AP. 00503

-78aTable 1-a (cont'd)

MELTING POINT/EMPIRICAL FORMULA

Cmpd No	Meltina Point (°C)	EmDirical Formula
142	SOLID	C15H18CI2N4
143	142-144	C15H19FN4
144	94-101	C16H21CIN4
145	135-137	C17H24N4O
146	SOLID	c14h17fn4
147	181-184 DEC	C14H16CI2N4
148	145-147	C16H17F5N4
149	143-146	C18H17F9N4
150	146-147	C15Hi6CIF3N4
151	118-119	C15H17F3N4O
152	172-174	C15H20N4O
153	148-150	O15H20N4S
154	82-85	C15H20N4O2S
155	237.5-238	C15H19N5O
156	148-151 DEC	O15H18CI2N4
157	166-171	C16H21CIN4O
158	174-176	C16H22N4O
159	135-136	C16H19F3N4O

AP. 00503

-79Insecticide Formulations

In the normal use of the insecticidal pyrimidines of the present invention, they usually will not be employed free from admixture or dilution, but ordinarily will be used In a suitable formulated composition compatible with the method of application and comprising an insecticidally effective amount of the pyrimidine. The pyrimidines of this invention, like most pesticidal agents, may be blended with the agriculturally acceptable surface-active agents and carriers normally employed for facilitating the dispersion of active ingredients, recognizing the accepted fact that the formulation and mode of application of an Insecticide may affect the activity of the material. The present pyrimidines may be applied, for example, as sprays, dusts, or granules to the area where pest control is desired, the type of application varying of course with the pest and the environment. Thus, the pyrimidines of this invention may be formulated as granules of large particle size, as powdery dusts, as wettable powders, as emulsifiable concentrates, as solutions, and the like. It will be understood that the insecticides themselves may be present as essentially pure compounds, or as mixtures of these pyrimidine compounds.

Granules may comprise porous or nonporous particles, such as

0 attapulgite clay or sand, for example, which serve as carriers for the pyrimidines. The granule particles are relatively large, a diameter of about ( 400-2500 microns typically. The particles are either impregnated with the pyrimidine from solution or coated with the pyrimidine, adhesive ( sometimes being employed. Granules generally contain 0.05-10%,

5 preferably 0.5-5%, active ingredient as the insecticidally effective amount.

Dusts are admixtures of the pyrimidines with finely divided solids such as talc, attapulgite clay, kleselguhr, pyrophyllite, chalk, diatomaceous earths, calcium phosphates, calcium and magnesium carbonates, sulfur, flours, and other organic and inorganic solids which acts carriers for the

0 insecticide. These finely divided solids have an average particle size of less than about 50 microns. A typical dust formulation useful for controlling insects contains 1 part of 2,4-diamino-5-[3-(2,4,5-trichlorophenyl)propyl]-6ethylpyrimidine (Compound 34) and 99 parts of talc.

The pyrimidines of the present invention may be made into liquid 3 5 concentrates by dissolution or emulsification in suitable liquids and into solid concentrates by admixture with talc, clays, and other known solid

AP/P; 9 3 / 0 0 5 9 5

AP. 00503

-80carriers used in the pesticide art. The concentrates are compositions containing, as an insecticidally effective amount, about 5-50% pyrimidine, and 95-50% inert material, which includes surface-active dispersing, emulsifying, and wetting agents, but even higher concentrations of active ingredient may be employed experimentally. The concentrates are diluted with water or other liquids for practical application as sprays, or with additional solid carrier for use as dusts.

Typical carriers for solid concentrates (also called wettable powders) include fuller's earth, clays, silicas, and other highly absorbent, readily wetted inorganic diluents. A solid concentrate formulation useful ‘ for controlling insects contains 1.5 parts each of sodium lignosulfonate and sodium lauryl sulfate as wetting agents, 25 parts of Compound 52 (above), and 72 parts of attapulgite clay.

Manufacturing concentrates are useful for shipping low melting

5 products of this invention. Such concentrates are prepared by melting the low melting solid products together with one percent or more of a solvent to produce a concentrate which does not solidify on cooling to the freezing point of the pure product or below.

Useful liquid concentrates include the emulsifiable concentrates,

0 which are homogeneous liquid or paste compositions readily dispersed in water or other liquid carriers. They may consist entirely of the pyrimidines ( with a liquid or solid emulsifying agent, or they may also contain a liquid carrier such as xylene, heavy aromatic naphthas, isophorone and other relatively non-volatile organic solvents. For application, these

5 concentrates are dispersed in water or other liquid carriers and normally applied as sprays to areas to be treated.

Typical surface-active wetting, dispersing, and emulsifying agents used in pesticidal formulations include, for example, the alkyl and alkylaryl sulfonates and sulfates and their sodium salts; alkylaryl sulfonates,

0 including fatty methyl taurides; alkylaryl polyether alcohols; sulfates of higher alcohols; polyvinyl alcohols; polyethylene oxides; sulfonated animal and vegetable oils; sulfonated petroleum oils; fatty acid esters of polyhydric alcohols and the ethylene oxide addition products of such esters; and the addition products of long-chain mercaptans and ethylene oxide. Many

5 other types of useful surface-active agents are available in commerce. The

AP 00503

-81 surface-active agent, when used, normally comprises about 1-15% by weight of the insecticidal composition.

Other useful formulations include simple solutions of the active ingredient in a solvent in which it is completely soluble at the desired concentrations, such as acetone or other organic solvents.

As shown in the biological test methods below, the compounds of the present invention were tested in the laboratory as dimethylsulfoxide solutions incorporated into an artificial insect diet or as aqueous acetone or methanol solutions containing a small amount of

0 octylphenoxypolyethoxyethanol surfactant for use as foliar sprays. An insecticidally effective amount of pyrimidine in an insecticidal composition diluted for application is normally in the range of about 0.001% to about 8% by weight. Many variations of spraying and dusting compositions known in the art may be used by substituting the pyrimidine of this invention into compositions known or apparent in the art.

The insecticidal compositions of this invention may be formulated with other active ingredients, including other insecticides, nematicides, acaricides, fungicides, plant growth regulators, fertilizers, etc. In using the compositions to control insects, it is only necessary that an insecticidally

0 effective amount of pyrimidine be applied to the locus where control is desired. Such locus may, e.g., be the insects themselves, plants upon ( which the insects feed, or the insect habitat. When the locus is the soil,

e.g., soil in which agricultural crops are or will be planted, the active ( compound may be applied to and optionally incorporated into the soil. For

5 most applications, an insecticidally effective amount will be about 75 to

4000 g per hectare, preferably 150 g to 3000 g per hectare.

Biological Data

The 5-substituted 2,4-diaminopyrimidines of the present invention

0 were incorporated into an artificial diet for evaluation of insecticidal activity against the tobacco budworm (Heliothis virescens [Fabricius]).

Stock solutions of test chemical in dimethylsulfoxide were prepared for each rate of application. The rates of application, expressed as the negative log of the molar concentration, and the corresponding

5 concentrations of the stock solution prepared for each rate are shown below:

AP/P/ 9 3 / 0 0 5 9 5

AP 00503

-82Stock Solution

Rale Qt Application micromolar 4

5

0.5 6

0.05 7

0.005 8 (

( 25

One hundred microliters of each of the stock solutions was manually stirred into 50 mL of a molten (65-70°C) wheat germ-based artificial diet. The 50 mL of molten diet containing the test chemical was poured evenly into twenty wells in the outer four rows of a twenty-five well, five row plastic tray. Each well in the tray was about 1 cm in depth, with an opening of 3 cm by 4 cm at the lip. Molten diet containing only dimethylsulfoxide at the levels used in the test chemical-treated diet was poured into the five wells in the third row of the tray. Each tray therefore contained one test chemical at a single rate of application, together with an untreated control.

Single second instar tobacco budworm larvae were placed in each well. The larvae were selected at a stage of growth at which they uniformly weigh about 5 mg each. Upon completion of infestation, a sheet of clear plastic was heat-sealed over the top of the tray using a common household flat iron. The trays were held at 25Cat 60% relative humidity for five days in a growth chamber. Lighting was set at 14 hours of light and 10 hours of darkness.

After the 5-day exposure period, mortality counts were taken, and the surviving insects were weighed. From the weights of the surviving insects that fed on the treated diet as compared to those insects that fed on the untreated diet, the percent growth inhibition caused by each test chemical was determined. From these data, the negative log of the concentration of the test chemical that provided 50% growth inhibition (pl50) was determined by linear regression, when possible, for each test chemical. Where possible, the negative log of the concentration of the test chemical that provided 50% mortality (PLC50) was also determined.

Generally, the compounds of the present invention inhibited the growth of tobacco budworm. The most efficacious compounds were 32,

34, 38, 37, 48, 50, and 56, 67, 71,74, 97,100, and 110-140, with pl₅₀

AP.00503

-83values of greater than 5. Compounds 34,110, 117, 118, 136-140, were the most active of these compounds with a ρίςο value of 6.4. All of the compounds exemplified above caused some insect mortality in this test. These data are presented in Table 2.

Certain 5-substituted-2,4-diaminopyrimidine derivatives with high pl50 values from the diet test were tested for insecticidal activity in foliar evaluations against the tobacco budworm, beet armyworm (Spodoptera exigua Hubner]), and the cabbage looper (Trichoplusia ni [Hubner]).

In these tests against the tobacco budworm and the beet armyworm, 1 0 nine-day-old chick pea plants {dear arietinum) were sprayed at 20 psi to runoff on both upper and lower leaf surfaces with solutions of test chemical to provide application rates as high as 1000 ppm of test chemical. The solvent used to prepare the solutions of test chemical was 10% acetone or methanol (v/v) and 0.1% of the surfactant octylphenoxypolyethoxyethanol

5 in distilled water. Four replicates, each containing four chick pea plants, for each rate of application of test chemical were sprayed. The treated plants were transferred to a hood where they were kept until the spray had dried.

The four chick pea plants in each replicate treated with test chemical 20 as described above were removed from their pots by cutting the stems just above the soil line. The excised leaves and stems from the four plants in ( each replicate were placed in individual 8-ounce paper cups, which contained a moistened filter paper. Five second-instar (4-5 days old) tobacco budworms or beet armyworms were counted into each cup, taking

5 care not to cause injury. An opaque plastic lid was placed on each cup, which was then held in a growth chamber for a 96 hour exposure period at 25°C and 50% relative humidity. At the end of the 96 hour exposure period the cups were opened, and the numbers of dead and live insects were counted. Moribund larvae which were disoriented or unable to crawl

0 normally were counted as dead. Using the insect counts, the efficacy of the test chemical was expressed in percent mortality. The condition of the test plants was also observed for phytotoxicity and for reduction of feeding damage as compared to an untreated control. Where applicable, computer-generated LC50 values were determined from the percentages

5 of insect mortality.

6 5 0 0 /26 ,'d/dV ^AP 00503

-84Foliar tests with cabbage looper were conducted in the same manner as described above, the difference being that pinto bean plants (Phaseolus vulgaris) were used.

Of the compounds evaluated on foliage for insecticidal activity, some of the more active ones were compounds 32, 34, 47, 50, 56, and more particularly, each of compounds 110-137 of Table 1. Of these, compound 34 was one of the most effective, providing excellent mortality of all three insect species in the foliar tests. These results are evident from the data in Table 3. Very good results with most of compounds 110-137,

0 which also represent preferred species of this invention, are likewise evident from this table.

Table 4, presents a comparison of the Insect Growth Inhibitory (INH) p>50 values from the diet test of certain compounds of the present invention with the percent mortality data for those same compounds in the foliar test.

These data clearly show that the compounds of the present invention with INH PI50 values of about 5.0 or higher are the most insecticidally active in the foliar test. Compound 34, with an INH plso ^va,ue θ·⁴> >ⁿ diet ^test provides excellent insect mortality in the foliar tests against tobacco budworm, beet armyworm, and especially cabbage looper. The results

0 obtained in the diet test are, thus, an excellent indication of the potential insecticidal efficacy of a compound when tested on the foliage of plants.

( The diet test as described herein when compared to a foliar test is a more precise means of testing compounds for insecticidal activity, and is not subject to environmental stresses that might be found in a greenhouse,

5 growth chamber, or from the whole plant itself. The results obtained from the diet test are more reproducible than those from a foliar test, and are based on quantitative measurement rather than observations.

A number of synthetic pyrethroids with known insecticidal activity in foliar tests against the tobacco budworm were also tested in the diet test

0 and were found to have INH ρΙςθ values of 6.6 or higher. These known synthetic pyrethroids are cypermethrin, permethrin, cyfluthrin, flucythrinate, and biphenthrin.

AP. Ο Ο 5 Ο 3

-85TABLE 2

INSECTICIDAL ACTIVITY OF 5-SUBSTITUTED-2.4DIAMINOPYRIMIDINES

INCORPORATED INTO THE DIET OF TOBACCO BUDWORM

CmpdNa.	Rate of ADDlication1	Percent Growth Inhibition^·4	0l505	Percent Mortality6	BLC507
1	7	ND		0	•
	6	ND
	5	ND		0
	4	10		0
	3.5	8		0
2	4	6	-	0	-
3	4	-4	-	0	-
4	8	ND		0	•
	7	ND
	6	ND
	5	-5
	4	13
5	7	ND		0	•
	6	ND
	5	ND
	4	-7
	3.5	18
6	8	ND	4.6	0	.
	7	ND		0
	6	-11		0
	5	25		0
	4	83		0
7	7	6	.	0
	6	-1		0
	5	-42		0
	4	-12		5
	3	-22		5
9	7	ND	3.6	0	•
	6	ND		0
	5	2		0
	4	29		5
	3.5	53		5

AP/P/ 9 3 / 0 0 5 9 5

AP.00505

	-86-
Table 2 (cont'd)
Cmpd No,	Rate of	Percent Growth		Percent
Application1	Inhibition^·4	-Pl505	Mortality6
10	7	ND	<3.5	0
	6	ND		0
	5	11		0
	4	11		5
	3.5	38		15
11	8	-20	.	5
	7	9		10
	6	3		5
	5	-5		0
	4	-5		0
12	8	11	.	0
	7	-5		0
	6	22		0
	5	-3		0
	4	-8		0
13	8	ND	4.7	0
	7	-11		0
	6	19		0
	5	34		0
	4	71		0
14	6	10	4.5	0
	5	23		0
	4	80		5
15	8	31	4.4	0
	7	23		0
	6	7		0
	5	14		0
	4	74		15
16	8	ND	-	0
	7	ND		0
	6	ND		0
	5	-1		0
	4	-3		0

ULCSO⁷

AP. 0 0 50 3

Table 2 (cont'd)
CmpdNa.	Rate of Application1	Percent Growth Inhibition2·3·4	Pl505	Percent Mortality6
17	8	ND	<3.5
	7	30		0
	6	34		0
	5	38		5
	4	32		5
	3.5	43		5
18	5	9	•	0
	4	15		0
19	4	-8	-	0
21	8	22	4.4	0
	7	22		0
	6	38		20
	5	42		0
	4	53		10
	3.5	58		10
22	8	21	4.3	0
	7	25		0
	6	25		0
	5	12		0
	4	70		15
	8	ND	.	ND
	7	ND		ND
	6	-28		0
	5	-14		0
	4	-11		0
23	8	-16	<4.0
	7	-20		0
	6	-15		0
	5	4		0
	4	42		20
24	7	ND	3.6	0
	6	10		0
	5	22		0
	4	33		5
	3.5	52		10

BLC50⁷

56500/26 /d/dV

AP. Ο Ο 5 Ο 3

Table 2 (cont’d)

-88Bl50⁵

Percent

Modality⁶ aLCso⁷

Cmod No. Rate of Application¹

Percent Growth lnHibition²·³·⁴

25	8 7 6 5 4	ND ND ND -15 16		0 0 0 0 0
26	5	-10	<4.0	0
	4	27		0
27	8	5	4.8	0
	7	17		0
	6	25		0
	5	38		0
	4	70		10
28	8	ND	<4.0	0
	7	ND		0
	6	trace		0
	5	4		0
	4	39		0
29	8	ND	<3.5	0
	7	ND		0
	6	17		0
	5	10		0
	4	31		0
	3.5	29		0
31	8	ND	4.8	0
	7	6		0
	6	19		0
	5	39		0
	4	74		0
32	7	16	5.2	0
	6	15		5
	5	66		25
	4	94		45
	3.5	97		55

3.8

Table 2 (cont’d)

CmpdNflx	Rate of Application1	Percent Growth Inhibition2·3·4	Bl505	Percent Mortality6
33	8	13	6.3	0
	7	29		0
	6	39		5
	5	93		0
	4	97		45
34	8	10	6.4	0
	7	-2		0
	6	89		30
	5	95		85
	4	100		85
35	8	35	4.6	0
	7	13		0
	6	20		0
	5	34		0
	4	73		5
36	7	3	3.6	0
	6	9		5
	5	9		10
	4	25		15
	3	85		10
37	8	ND	5.1	0
	7	-29		0
	6	38		0
	5	42		0
	4	76		0
38	6	-4	4.9	0
	5	56		0
	4	88		0
	8	15	5.9	0
	7	21		0
	6	34		0
	5	71		0
	4	93		15

pLCso⁷

5.7 s 6 S 0 0 / ί 6 /d/dV

AP. 00503

Table 2 (cont'd)	Percent Growth Inhibition2·3·4	Bl505	Percent Mortality6
Cmpd No,	Rate of Application1
39	6	9	5.1	0
	5	63		0
	4	91		20
40	8	4	-	0
	7	4		0
	6	20		5
	5	2		5
	4	18		0
41	8	ND	4.7	0
	7	ND		0
	6	1		0
	5	39		0
	4	73		0
42	8	4	.	0
	7	11		0
	6	15		5
	5	-4		5
	4	-14		5
43	8	-8	5.1	0
	7	2		0
	6	12		5
	5	54		0
	4	ND		ND
	8	58	-	0
	7	35		5
	6	32		5
	5	49		5
	4	33		5
	8	38	.	0
	7	43		0
	6	23		0
	5	34		5
	4			5
	7	30	3.0	0
	6	16		0
	5	1		0
	4	26		0
	3	51		0

DLC50⁷

AP

0 5 0 3

-91 Table 2 (cont'd)

Cmod No.

Rate of Application1	Percent Growth InUituiion2·3·4	J2l505	Percent MfiOaiiix6	BlXsO7
8	ND	4.9	0	-
7	1		0
6	15		0
5	41		0
4	80		15
8	19	3.6	0	.
7	14		0
6	24		10
5	35		5
4	42		5
3.5	54		5
8	11	<4.0	0
7	0		0
6	-7		0
5	-2		0
4	42		0
8	6	5.9	0	<4.0
7	-17		0
6	66		5
5	90		20
4	95		30
8	10	6.1	0	4.1
7	22		0
6	50		5
5	76		15
4	98		60
3.5	98		70
8	2	6.0	0	4.4
7	10		0
6	60		10
5	85		20
4	98		70
8	7	4.5	0
7	-22		0
6	-16		0
5	20		0
4	84		15

AP/P/ 9 3 / 0 0 5 9 5

W Μ 5 0.1

-92Table 2 (cont’d)

Cmpd No.	Rate of Aoplicationl	Percent Growth Inhibition2·3·4	Bl505	Percent Mortality6	fiLCso7
50	8	21	6.0	0	<4.0
	7	23		0
	6	37		5
	5	75		5
	4	94		30
51	8	-29	4.9	0	-
	7	12		5
	6	14		0
	5	30		5
	4	88		15
52	8	ND		0	-
	7	ND		0
	6	ND		0
	5	-2		0
	4	20		0
53	8	ND	4.9	0	-
	7	ND		0
	6	4		0
	5	50		0
	4	80		0
54	6	6	5.4	0	<4.0
	5	82		20
	4	98		40
	8	-1	5.7	0	-
	7	22		0
	6	33		0
	5	73		0
	4	92		15
55	6	4	4.9	0	-
	5	59		0
	4	89		5

AP η η 5 ό 5

-93Table 2 (cont'd)

Cmpd No,	Rate of Application1	Percent Growth Inhibition2·3·4	Bl505	Percent Mortality6	&LC507
56	8	-7	5.7	0	-
	7	-19		0
	6	33		0
	5	83		15
	4	91		20
57	6	-3	4.3	0	-
	5	18		0
	4	62		0
58	4	3	-	0	-
59	5	18	4.2	0	-
	4	57
60	6	-1	4.3	0	.
	5	15		0
	4	66		0
61	6	-24	4.4	0	•
	5	10		0
	4	79		10
62	5	4	4.3	0	-
	4	71		0
63	6	8	4.2	0	.
	5	18		0
	4	60		0
64	5	-7	•	0	.
	4	13		0
65	5	1	4.3	0	•
	4	72		5
66	5	-8	.	0	-
	4	11		0

S 6 S 0 0 / £ 6 /d/dV

AP 00503

Table 2 (cont’d)
Cmod No.	Rate of	Percent Growth		Percent	Bk£c507
Aoplication1	Inhibit ion2.3,4	Bl505	Mortality6
67	7	13	5.9	0	<4.0
	6	40		0
	5	83		10
	4	95		25
69	5	-5	4.1	0	.
	4	56		0
70	5	1	-	0	-
	4	13		0
71	7	13	4.9	0	<4.0
	6	9		0
	5	28		0
	4	93		30
	7	20	5.6	0	.
	6	43		0
	5	54		0
	4	90		15
72	5	26	<4.0	0
	4	29		0
74	6	8	5.1	0	<4.0
	5	67		0
	4	95		30
75	4	TRACE	-	0	-
77	5	-10	<4.0	0
	4	28		0
78	6	24	4.0	0	-
	5	19		0
	4	48		0
79	6	4	4.9	0	-
	5	49		0
	4	83		10

AP ο π 5 f) j

-95Table 2 (cont'd)

Cmod No.	Rate of Application1	Percent Growth Inhibition2’3·4	Bl505	Percent Mfidaiilx6	BL£50
80	5	-4	>4.0	0	-
	4	31		0
82	4	1	-	0	-
83	4	-15	-	0	-
84	5	3	.	0	-
	4	2		0
85	5	-1	<4.0	0
	4	21		0
86	5	-5		0	-
	4	12		0
87	4	12	-	0	-
88	5	26	<4.0	0
	4	35		0
90	5	3	-	0	-
	4	1		0
91	4	2	-	0	-
92	6	-7	4.7	0	-
	5	23		0
	4	86		0
93	4	14	-	0	-
96	5	16	4.4	0	-
	4	74		0
97	8	-7	5.7	0	.
	7	-19		0
	6	33		0
	5	83		15
	4	91		20

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-96Table 2 (cont'd)

Cmpd bio.	Rate of Application1	Percent Growth Inhibition2^·14	Bl505	Percent Mortality
98	6	10	4.7	0
	5	33		0
	4	76		10
99	6	-1	4.3	0
	5	15		0
	4	66		0
100	7	-14	5.0	0
	6	24		0
	5	37		0
	4	88		5
101	4	-7	-	0
102	5	12	4.1	0
	4	52		0
103	6	-16	•	0
	5	7		0
	4	18		0
104	6	-24	4.4	0
	5	10		0
	4	79		10
105	4	-8	-	0
106	5	9	<4.0	0
	4	22		20
107	4	8	-	0

PLCSO⁷

AP. 00503

-97Table 2 (cont'd)

Cmpd Nq.	Rate of ADDlication1	Percent Growth Inhibition2·3·4	Pl505	Percent Monaiiix6	PLGSO
108	6	13	4.9	0	*
	5	46		0
	4	83		0
109	4	-23	-	0	-
110	7	27	6.6	0	.
	6	88		15
	5	96		35
	4	100		95
111	6	35	5.3	0
	5	60		0
	4	75		0
112	8	23	5.6	0	<4.0
	7	10		0
	6	31		0
	5	76		5
	4	90		25
113	8	2	5.1	0	-
	7	25		0
	6	14		0
	5	66		5
	4	82		15
114	7	9	5.8	0	4.4
	6	35		5
	5	84		10
	4	98		80
115	6	9	5.3	0	<4.0
	5	63		15
	4	97		35
116	6	16	5.3	0	.
	5	79		5
	4	95		20

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-98Table 2 (cont'd)

Cmpd No.	Rate of Application1	Percent Growth Inhibition2·3·4	-Pl505	Percent Modality6	QLCso7
117	7	16	6.6	0	5.5
	6	92		35
	5	98		65
	4	99		70
118	7	17	6.5	0	4.8
	6	88		15
	5	97		55
	4	99		65
119	7	8	5.0	0	-
	6	7		0
	5	59		0
	4	85		10
120	7	11	5.9	0	<4.0
	6	45		5
	5	84		10
	4	96		30
121	6	9	5.2	0	<4.0
	5	73		5
	4	94		25
122	7	29	5.4	0	<4.0
	6	23		0
	5	75		5
	4	96		40
123	7	-13	5.8	0	-
	6	48		0
	5	88		20
	4	94		10
124	6	13	5.2	0	<4.0
	5	63		15
	4	97		35

ΛΡ 00505

-99Table 2 (cont'd)

Cmpd No.	Rate of Application1	Percent Growth Inhibition2’3,4	-Bl505	Percent Mortality6	BLC50
125	7	-14	5.7	0	<4.0
	6	41		0
	5	85		10
	4	95		30
126	6	16	5.3	0	<4.0
	5	79		0
	4	97		30
127	6	25	5.6	0	-
	5	84		0
	4	93		5
128	7	7	5.9	0	-
	6	47		0
	5	89		25
	4	95		20
129	6	-6	5.1	0	-
	5	78		5
	4	92		10
130	6	7	5.2	0	.
	5	76		5
	4	94		15
131	6	9	5.5	0	-
	5	86		20
	4	94		25
132	7	29	6.3	0	.
	6	55		0
	5	91		0
	4	96		5
133	6	18	5.0	0
	5	53		0
	4	83		5

AP/P/ 9 3 / 0 0 5 9 5

AP η Ο 5 0 J

-100-

Table 2 (cont'd)
Cmpd No,	Rate of Application1	Percent Growth Inhibition2·3·4	P1505	Percent Modality6	PLC507
134	6	16	5.5	0	-
	5	84		0
	4	93		0
	7	9	5.9	0	-
	6	43		0
	5	84		5
	4	92		15
135	7	10	6.2	0	<4.0
	6	72		0
	5	91		20
	4	93		25
136	8	20	7.0	0	4.7
	7	45		0
	6	88		20
	5	97		40
	4	99		70
	8	14	7.0	0	4.8
	7	47		0
	6	89		20
	5	98		35
	4	100		80
137	7	-1	6.4	0	4.4
	6	80		10
	5	95		25
	4	98		65
138	8	-5	6.4	0	5.1
	7	34		0
	6	58		5
	5	94		55
	4	96		60

Table 2 (cont’d)

AP·. 0 0 5 0 3

-101 -

Cmod No.	Rate of Application1	Percent Growth Inhibition2-3·4	Cl505	Percent Mortality6	BL£507
139	7	19	6.5	0	<4.0
	6	83		0
	5	94		10
	4	96		35
	8	6	6.7	0	4.4
	7	21		5
	6	83		15
	5	95		25’
	4	99		70
140	8	5	6.7	0	4.5
	7	27		0
	6	83		10
	5	93		10
	4	100		95
	8	1	6.7	0	4.6
	7	24		0
	6	82		10
	5	96		30
	4	99		70

Footnotes - Table 2 ¹ The rate of application is expressed as the negative log of the molar concentration of the test compound in the diet.

² Percent growth inhibition is derived from the total weight of the insects (IW) at each rate of application in the test relative to the total weight of insects in an untreated control,

IW (control) - IW (test) % Gr. Inh. = - x 100

IW (control) ³ ND = no data ⁴ A minus % growth inhibition indicates that the insects weighed more at the termination of the test than those in the untreated control.

⁵ P>50 *s the negative log of the concentration of the test chemical that provides 50% growth inhibition of the test insects.

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-1025 pl50'^s negative log of the concentration of the test chemical that provides 50% growth inhibition of the test insects.

Percent mortality is derived from the total number of dead insects (TD) relative to the total number of insects (Tl) used in the test.

TD % mortality = - X 100

Tl

PLC50 is the negative log of the concentration of the test chemical that provides 50% mortality of the test insects.

AP. 00503

-102aInsecticidal Activity of 5-Substituted-2,4-diaminopyrimidines Incorporated into the Diet of Tobacco Budworm

Table 2-a

Cmpd, Nq.	Rate of Application1	Percent Growth Inhibition2·3	Pl504	Percent Mortality5	PLC506
142	7	6	5.4	0	<4.0
	6	23		0
	5	73		0
	4	91		30
143	7	-2	6.0	0	4.5
	6	59		0
	5	92		30
	4	99		70
144	7	-13	5.6	0
	6	24		0
	5	80		15
	4	95		20
145	6	-5	4.8	0	---.
	5	35		10
	4	86		20
146	6	76	>6.0	0	<4.0
	5	88		5
	4	95		35
	7	-6	5.4	0	....
	6	12		0
	5	72		10
	4	75		10
	7	-11	5.2	0
	6	13		0
	5	77		15
	4	90		15
147	7	11	6.0	0	4.6
	6	45		0
	5	93		25
	4	98		75

AP/P/ 9 3 / 0 0 5 9 5

AP. Ο η 5 Ο 3

-102bTable 2-a (cont'd)

CmDd. No.	Rate of ADDlication1	Percent Growth Inhibition2·3	Ul504	Percent Mortality5
148	6	35	5.2	0
	5	50		0
	4	82		15
149	6	14	4.9	0
	5	47		0
	4	83		10
150	7	9	6.4	0
	6	83		20
	5	98		35
	4	99		45
151	6	2	5.1	0
	5	71		10
	4	95		15
152	7	11	6.0	0
	6	54		0
	5	83		0
	4	94		15
153	7	17	5.9	0
	6	41		0
	5	83		0
	4	97		30
154	6	-1	4.9	0
	5	43		5
	4	88		35
155	6	23	5.6	0
	5	84		15
	4	95		15
156	7	10	6.1	0
	6	60		5
	5	86		30
	4	96		40

2LQ50⁶ <4.0 <4.0 <4.0 <4.0

AP. Ο Ο 50 J

-102cTable 2-a (cont’d)

Cmpd, No.	Rate of ADDlication1	Percent Growth Inhibition2·3	Ul504	Percent Mortality5	βΙ£506
157	7	8	5.7	0	-—
	6	26		0
	5	82		5
	4	94		5
158	7	11	5.7	0
	6	37		0
	5	79		0
	4	92		0
159	6	13	5.2	0	....
	5	64		0
	4	93		5

FOOTNOTES ¹ The rate of application is expressed as the negative log of the molar concentration of the test compound in the diet.

² Percent growth inhibition is derived from the total weight of the insects (IW) at each rate of application in the test relative to the total weight of insects in an untreated control,

C % Gr. Inh. = [IW (control) - IW (test) / IW (control)] x 100

C ³A minus % growth inhibition indicates that the insects weighed more at the termination of the test than those in the untreated control.

A pl5Q is the negative log of the concentration of the test chemical that provides 50% growth inhibition in the test insects.

⁵ Percent mortality is derived from the number of dead insects (TD) relative to the total number of insects (Tl) used in the test, % Mortality = yp x 100 ⁶ PLC50 is the negative log of the concentration of the test chemical that provides 50% mortality of the test insects.

AP/P/ 9 3 / 0 0 5 9 5 ^ΑΡ· 00 5 0 J

-103 iablej

INSECTICIDAL ACTIVITY OF 5-SUBSTITUTED-2.4DIAMINOPYRIMIDINES APPLIED AS FOLIAR SPRAYS

Percent Mortality¹

Cmpd Nth	Rate of Application (ppm)	TBW	BAW	CL
14	1000	50
	300	26
	100	5
	30	5
	10	5
17	300	0
	100	0
	30	0
	10	0
	3	0
21	1000	10
32	1000	100
	300	85
	100	70
	30	20
	10	0
34	1000		100 (100)2
	300	100	95 (100)	100
	100	100	90 (95)	95 (100)
	30	90	15 (50)	80 (100)
	10	60	10(5)	80 (75)
	3	15		60 (55)
	1			(10)
35	300	10
	100	0
	30	5
	10	0
	3	0
38	1000			95
	300	50		60
	100	47		25
	30	15		5
	10	16		0

Table 3 (cont'd)

AP On so 3

-104Percent Mortality¹

Cmpd Nq,	Rate of Application (ppm)	IEW		CL
43	1000	10		15
	300	5
	100	5
	30	5
	10	0
45	300	0
	100	0
	30	0
	10	0
	3	0
47	1000	100
	300	87	15
	100	79	0
	30	30	0
	10	11	0
48	300	100 (95)
	100	25 (78)
	30	0(37)
	10	0(5)
	3	0(11)
50	1000	95		55
	300	60
	100	25
	30	10
	10	5
56	300	95
	100	25
	30	22
	10	0
	3	5

AP/P/ 9 3 / 0 0 5 9 5

AP . 00503

-105Table 3 (cont’d)

Percent Mortality¹

Rate of

CmDd No. ADDlication (Dam)	IBW	BAW	£L
62 3000	5	0	16
1000	0	0	5
300	0	0	10
100	0	0	0
30	0	0	15
65 3000	80	0	58
1000	11	0	33
300	5	0	32
100	0	0	17
30	0	0	16
3000			33
1000			20
300			10
100			15
30			0
66 1000		0	10
300	5	0	10
100	0	0	16
30	0	0	5
10	0	0	0
3	0
67 3000			100
1000	80	45	100
300	42	0	95
100	11	0	55
30	0	0	25
10	0	0
3000		95
1000		85
300		15
100		0
30		0

AP. η η 5 ο 3

Table 3 (cont'd)

-106_Percent Mortality¹

Rate of

Cmpd No,	Application (ppm)	TBW		£L
69	1000	25	0	45
	300	0	0	20
	100	0	0	0
	30	0	0	0
	10	0	0	11
70	1000		0	16
	300	6	0	5
	100	5	0	0
	30	0	0	0
	10	5	0	5
	3	5
71	1000	75
74	3000	54	95	70
	1000	22	20	65
	300	5	5	50
	100	0	0	25
	30	0	0	25
77	1000	0	0	22
	300	0	0	16
	100	0	0	0
	30	5	0	5
	10	5	0	0
78	3000	0	0	20
	1000	0	0	28
	300	0	0	37
	100	0	0	22
	30	0	0	26
	3000			21
	1000			6
	300			6
	100			6
	30			10
	3000			25
	1000			5
	300			16
	100			5
	30			15

AP/P/ 9 3 / 0 0 5 9 5

AP. Ο η 5 ο 3 •107_ Percent Mortality¹

Rate of

Table 3 (cont’d)

Cmpd No, Application (pam)	IBW	BAW	£L
79 3000	15	84	33
1000	0	42	47
300	0	10	35
100	5	0	20
30	11	0	6
3000			65
1000			55
300			40
100			30
30			25
90 1000		0	0
300	5	0	0
100	0	0	0
30	5	0	0
10	6	0	0
3	5
96 3000	0	0	50
1000	11	0	45
300	0	0	40
100	0	0	15
30	0	0	15
97 1000	95	95	89
300	95	30	65
100	60	10	20
30	16	0	5
10	5	0	5
300	95
100	25
30	22
10	0
3	5

AP. Ο Π 5 Ο 5

Table 3 (cont'd)

-108 _Percent Mortality¹

Rate of

Cmpd No, Application (ppm)	TBW	BAW	CL
99	1000	0	0	15
	300	0	0	21
	100	0	0	20
	30	0	0	0
	10	0	0	10
100	3000	50	16	20
	1000	20	0	5
	300	0	0	5
	100	11	0	10
	30	0	0	5
102	3000	0	5	11
	1000	0	0	10
	300	0	0	10
	100	0	0	15
	30	0	0	5
104	1000	25	0	20
	300	0	0	10
	100	0	0	10
	30	5	0	5
	10	0	0	5
110	1000	100	100	100
	300	100	100	100
	100	100 (100)	85	70 (85)
	30	95 (95)	75	65 (75)
	10	78 (80)	6	85 (65)
	3	(16)		(25)
	1	(0)		(5)
114	3000	100	95	100
	1000	100	70	100
	300	100 (100)	10	95
	100	85 (95)	0	75
	30	68 (40)	0	44
	10	(10)
	3	(0)

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-109Table 3 (cont'd)

Rate of

Cmpd No, Application (ppm)

Percent Mortality¹ tbw BAyy cl

115

116

117

118

120

3000	100
1000	94
300	39 (58)	5	75
100	25 (5)	0	70
30	11 (5)	0	55
10	(6)	0	15
3	(15)	0	11
3000	100	100	100
1000	88	80	100
300	33 (21)	37	95 (85)
100	25 (5)	5	61 (63)
30	11 (5)	5	56 (32)
10	(10)		(25)
3			(16)
300	100	100	95
100	100	90	90
30	95	45	90
10	53	5	80
3	3	0	10
1000	100	100
300	100	100 (95)	100
100	95	80 (65)	89 (100)
30	83	20 (26)	95 (85)
10	53	5 (0)	95 (75)
3		(0)	42 (33)
1			(20)
1000	100	100 (5)	95
300	100	11 (0)	70
100	95	10 (0)	55
30	60	0 (0)	37
10	6	0 (0)	5
3000	100	95 (79)	100
1000	100	16 (40)	74
300	85	0 (5)	65
100	38	0 (0)	35
30	20	0 (0)	15

121

AP, 00503

Table 3 (cont'd)

-110_Percent Mortality¹

Rate of

Cmpd Nth	Application (ppm)	TBW	BAW	CL
122	1000	100	100	100
	300	100 (95)	82	100
	100	75 (60)	11	75
	30	30 (6)	11	30
	10	11 (5)	10	11
	3	(0)
123	1000	100	95	89
	300	100	32	75
	100	85	5	12	in
	30	56	0	16	cn
	10	12	0	0	m
					o
124	1000	95	0	75
	300	85	0	35
	100	45	0	20
	30	17	0	10
	10	0	0	15
125	1000	100	44	45	Ql
	300	100	15	58	CU
	100	85	0	40	<
	30	56	5	5
	10	12	0	5
128	3000		95	100
	1000	100	20 (55)	95 (33)
	300	100	5 (15)	80 (50)
	100	89	0 (0)	70 (20)
	30	45	0 (0)	20 (20)
	10	5	(0)	(15)
130	3000	100	20	90
	1000	88	0	60
	300	33	0	5
	100	11	0	10
	30	5	0	10
131	3000	100	95	90
	1000	90	50	95
	300	72	5	55
	100	10	0	25
	30	5	0	0

AP. 00503

- 111 Table 3 (cont’d)

Percent Mortality¹

Rate of	fiAW	CL
Cmpd No. ADplication (DDm)	IBW
133 3000	85	85	100
1000	70	16	90
300	30	10	65
100	5	0	40
30	0	10	20
135 3000			95
1000	89	89	65 (29)
300	74	50	42 (47)
100	25	5	26 (25)
30	0	0	10 (30)
10	0	0	(5)
136 1000	100	100	100
300	100	100	100
100	100	89	100
30	100 (100)	60	95 (100)
10	100 (90)	28	95 (90)
3	(67)		(90)
1	(16)		(45)
0.3	(0)		(25)
139 1000	100	100	100
300	100	100	100
100	100 (100)	75	100 (100)
30	100 (95)	45	100 (95)
10	95 (50)	5	95 (90)
3	(20)		(60)
1	(26)		(16)
140 1000	100	100	100
300	100	100	100
100	100	95	95
30	95 (90)	40	85 (95)
10	95 (68)	15	90 (95)
3	(26)		(75)
1	(6)		(25)
0.3	(5)		(5)
1 tobacco budworm - TBW
beet armyworm - BAW
cabbage looper - CL

The numbers in parenthesis represent the results of a second test

AP . 0 0 5 0 3

-111aTable 3 (cont'd)

Insecticidal Activity of 5-Substituted-2,4-diaminopyrimidines Applied as Foliar Sprays

CmDd No.	Rate of Application (Ppm)	Percent Mortality
TBW		BAW
143	1000	100	89	100
	300	100	95	95
	100	100	75	25
	30	79	58	5
	10	74	10	11
	100	100
	30	95
	10	47
	3	15
	1	0
146	1000	100	47	84
	300	90	16	32
	100	79	5	5
	30	18	0	0
	10	5	0	0
147	1000	100	100	89
	300	100	40	10
	100	94	95	5
	30	72	65	0
	10	15	25	0
	1000		95	100
	300		60	74
	100		40	5
	30		40	5
	10		32
	1000		95
	300		78
	100		80
	30		85
	10		35

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-111bTable 3 (cont'd)

CmDd No.	Rate of Application (ppm)	Percent Mortality
TPW	CL	PAW
148	1000	75	21	53
	300	40	20	5
	100	5	5	0
	30	5	5	0
	10	0	10	0
149	3000	55	89	25
	1000	25	26	5
	300	5	15	0
	100	0	5	5
	30	0	5	0
150	1000	100	100	100
	300	100	89	100
	100	100	80	95
	30	94	95	20
	10	100	80	0
	100	100	75	95
	30	95	67	94
	10	84	70	10
	3	5	30	5
	1	0	5	0
151	3000	100	100	95
	1000	80	75	80
	300	63	80	10
	100	5	60	0
	30	15	10	0
152	1000	100	95	95
	300	75	76	53
	100	79	84	11
	30	39	10	0
	10	10	10	16
153	3000	100	89	95
	1000	83	50	58
	300	68	45	16
	100	25	17	0 ...
	30	7	0	0

AP. 00503

-111cTable 3 (cont'd)

Cmpd No,	Rate of Application (ppm)	Percent Mortality
ISW	CL	BAVV
154	3000	79	30	26
	1000	11	0	0
	300	6	5	5
	100	0	15	0
	30	0	10	11
159	3000	32	85	47
	1000	26	75	15	to
	300	5	60	0
	100	11	20	5	o>
	30	5	5	5	to

<o to db £

£ <

( (

AP . 0 0 so 3

-112TABLE.4

CORRELATION OF GROWTH INHIBITION PI50 VALUES FROM THE DIET TEST WITH TOBACCO BUDWORM MORTALITY IN THE FOLIAR TEST¹

Cmpd	Growth Inhibition		Folia	rTest
	-Bl50—	Rate of		Mortality
14	4.5	1000	PPm	50
		300		26
17	<3.5	300		0
21	4.4	1000		10
32	5.2	1000		100
		300		85
34	6.4	300		100
		100		100
35	4.6	300		10
		100		0
38	4.9 (5.9)3	300		50
		100		47
43	5.1 (3.0)	1000		10
	300		5
45	3.6	300		0
47	5.9 (6.1)	1000		100
	300		87
48	6.0	300		100 (95)
		100		25 (78)
50	6.0	1000		95
		300		60
56	5.7	300		95
		100		25

¹ These data were compiled from Tables 2 and 3.

² The two highest rates of application, or the only rate ot application tested are shown here.

The number in the parenthesis is a value derived from a second test.

Claims (10)

1 5 wherein

V, W, X, Υ, and Ζ are independently hydrogen, halo, lower alkyl, lower alkoxy, trihaloalkyl, nitro, phenyl, or (substituted-phenyl lower alkyl)amino, wherein at least one of V to Z is not hydrogen;

n is 0 to 10;

R¹ is hydrogen, lower alkyl, or amino;

R² is hydrogen, or lower alkyl; and

R³ is hydrogen.

1 Ο (

C

1 5

1 0 (

f

1. An insecticidal composition comprising, in admixture with an agriculturally acceptable carrier and a surface-active agent, an insecticidally effective amount of a compound characterized by the formula:

(D wherein R is (a) phenyl, or substituted-phenyl of the formula:

AP/P/ 9 3 / 0 0 5 9 5 wherein

V is hydrogen, halogen, lower alkyl, or lower haloalkyl;

W is hydrogen, halogen, straight or branched chain alkyl, alkylsilane, aikylsilanylalkyl, phenyl, alkoxy, haloalkyl, pyridinyl alkyl, nitro, substituted- phenylaikyi, cyano, aminocarbonyl, phenyl(hydroxyaikyl), substitutedphenoxyalkyi, bicycioalkoxy, -NHC(O)R⁴, or -NHR5, wherein R⁴ is alkyl, haloalkyl, substituted-phenyl, substituted-phenylaikyl, or substituted-phenoxyalkyi, R³ is alkyl, cycloalkyl, substituted-phenylaikyl, substitutedphenoxyalkyi, or pyridinylalkyl, and wherein the phenyl substituents are halo, alkoxy, or aikylsulfonyl;

AP. ¢+503

-114X is hydrogen, halogen, lower alkyl, lower alkoxy, alkoxycarbonyl, alkylthio, alkylsulfonyl, lower haioalkyl, aminocarbonyl, lower haloalkylsulfonyl, lower haloalkoxy, cyano, lower alkylcarbonylamino, (phenyl or substitutedphenyl lower alkyl)amino or (lower alkyl)(phenyl, or substituted-phenyl lower alkyl)amino, wherein the phenyl, when substituted, is substituted with halogen, lower alkoxy, lower alkyl, or lower haioalkyl;

Y is hydrogen, halogen, lower alkyl, lower haioalkyl, or lower alkoxy; and

Z is hydrogen, halogen, or lower alkyl; or (b) substituted-phenyl lower alkyl of the formula:

( wherein

C q is 1 or 2; and,

V¹· W¹, X¹, Y1 and Z¹ are independently selected from hydrogen, lower alkyl, halogen, lower alkoxy, lower haioalkyl, lower alkylsulfonyl, cyano, or aminocarbonyl; or (c) naphthyl or substituted-naphthyl of the formula:

(IV) wherein

V². W², X², Y^2, and Z² are independently selected from halogen, lower alkyl, lower haloalkyl, lower aJkylsulfonyl, cyano, or aminocarbonyl; or (d) thiazol-2-yl, benzothiazol-2-yl, or 5-substituted-thien-2-yl of the formula:

ΑΡ. ΠΠ503

-115V³ (V) wherein

V³ is halogen, lower alkyl, lower haloalkyl, cyano, or aminocarbonyl;

and wherein

R1 is hydrogen, lower alkyl, amino, phenyl, or phenyl lower alkyl;

R2 is hydrogen or lower alkyl;

R3 is hydrogen or lower alkyl;

or R2 and R3, taken together, form the group -R6-O-R6-, wherein R6 is lower alkylene;

( m is 0 or 1;

n is 2 to 11; and p is 0 or 1, and agriculturally acceptable salts thereof.

2. The composition of claim 1 characterized in that m and p are 0;

R is naphthyl or substituted-naphthyl, phenyl or substituted-phenyl of the structure

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

116V

Y (Π)

3. The composition of claim 1 characterized in that m is 1 and p is 0;

R is phenyl;

n is 2 or 3;

R¹ is hydrogen, lower alkyl, or amino; and

R² and R³ are hydrogen.

4. The composition of claim 1 characterized in that m and p are 1;

R is substituted-phenyl of the structure:

(Π) wherein

W, X, Y, and Z are hydrogen, and V is trihaloalkyl;

AP. 00503

-117n is

R¹ is lower alkyl; and

R2 and R3 are hydrogen, lower alkyl, or amino.

5. The composition of claim 1 characterized in that m and p are 0; n is 2;

R is substituted-phenyl lower alkyl of the formula;

x’ (III) wherein q is 1, W¹ or X¹ is trihaloalkyl, and wherein the remaining substituents V¹, W¹, X¹, Y¹, or Z¹ are hydrogen or halo;

R1 is lower alkyl; and

R2 and R3 are hydrogen.

6. The composition of claim 1 characterized in that m and p are 0; n is 3 or 4;

R is naphth-1-yl, or substituted-phenyl of the structure:

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-118- ,w

X

Y (Π) wherein

V is lower alkyl or trihaloalkyl; W is phenyl; X is halo or lower alkyl; or V, X, and Y are halo or lower alkyl; or V and X are halo; and the remaining substituents V, W, X, Y, and Z are hydrogen;

Rl is hydrogen, lower alkyl, or amino;

R² is hydrogen or lower alkyl; and

R³ is hydrogen.

7. The composition of claim 7 characterized in that V, X, and Y are all chloro or all methyl, and the remaining substituents are hydrogen.

8. The composition of claim 1 characterized in that m is 1; n is 2 or 3; p is 0;

R is phienyl;

R¹ is hydrogen or lower alkyl; and

R² and R³ are hydrogen.

9. The composition of claim 1 characterized in that m and p are 1 ; n is 3;

R is substituted-phenyl of the structure:

AP. 00503 ( 15

χ._ wherein

V is trihaloalkyl, and the remaining substituents W, X, Y, and Z are hydrogen;

R1 is lower alkyl; and

R² and r3 are hydrogen.

10. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 1.

11. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 2.

12. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 3.

13. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 4.

14. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 6.

15. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 7.

16. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 8.

AP/P/ 9 3 / 0 0 5 9 5

AP. 00503

-12017. A method for controlling insects characterized by applying to the locus where control is desired an insecticidal amount of the composition of claim 9.

ie. A method for controlling insects characterized by applying to 5 the locus where control is desired an insecticidal amount of the composition of claim 10.

19. The composition of claim 1 characterized in that the insecticide is 2,4-diamino-5-[3-(2,4-dichlorophenyl)propylJ-6methylpyrimidine.

10 20. A method for controlling insects characterized in that applying to the locus where control is desired an insecticidal amount of the composition of claim 21.