ZA954983B - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
ZA954983B
ZA954983B ZA954983A ZA954983A ZA954983B ZA 954983 B ZA954983 B ZA 954983B ZA 954983 A ZA954983 A ZA 954983A ZA 954983 A ZA954983 A ZA 954983A ZA 954983 B ZA954983 B ZA 954983B
Authority
ZA
South Africa
Prior art keywords
yeast
proteins expressed
terminally extended
extended proteins
terminally
Prior art date
Application number
ZA954983A
Inventor
Knud Vad
Jakob Brandt
Thomas Borglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of ZA954983B publication Critical patent/ZA954983B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
ZA954983A 1994-06-17 1995-06-15 N-terminally extended proteins expressed in yeast ZA954983B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17

Publications (1)

Publication Number Publication Date
ZA954983B true ZA954983B (en) 1996-02-14

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA954983A ZA954983B (en) 1994-06-17 1995-06-15 N-terminally extended proteins expressed in yeast

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
DE69739319D1 (en) * 1996-12-13 2009-04-30 Novartis Vaccines & Diagnostic Method for expression of PDGF or IGR proteins in yeast
EP0946735B1 (en) * 1996-12-20 2006-02-08 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
AU2149899A (en) * 1998-01-23 1999-08-09 Novo Nordisk A/S Process for making desired polypeptides in yeast
EP1246933B1 (en) 1999-12-29 2010-07-21 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
EP1421102A2 (en) * 2001-07-24 2004-05-26 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
CN102816228A (en) 2003-12-03 2012-12-12 诺和诺德公司 Single-chain insulin
CN102660614A (en) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 Method for making mature insulin polypeptides
ES2542146T3 (en) * 2006-07-31 2015-07-31 Novo Nordisk A/S PEGylated extended insulin.
CN101541830A (en) 2006-09-22 2009-09-23 诺沃-诺迪斯克有限公司 Protease resistant insulin analogues
US8518668B2 (en) 2006-09-27 2013-08-27 Novo Nordisk A/S Method for making maturated insulin polypeptides in a fungal cell
ES2425413T3 (en) 2006-11-22 2013-10-15 Novo Nordisk A/S Method for preparing activated carboxypeptidase
JP5688969B2 (en) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス Pegylated insulin analogues are stable against proteases
CN101778862B (en) 2007-08-13 2014-12-17 诺沃-诺迪斯克有限公司 Rapid acting insulin analogues
WO2009022006A1 (en) 2007-08-15 2009-02-19 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
MX2010001645A (en) 2007-08-15 2010-03-10 Novo Nordisk As Insulin analogues with an acyl and aklylene glycol moiety.
RU2571857C2 (en) 2008-03-18 2015-12-20 Ново Нордиск А/С Acylated insulin analogues stabilised with respect to proteases
US9242011B2 (en) 2008-04-01 2016-01-26 Novo Nordisk A/S Insulin albumin conjugates
ES2543705T3 (en) 2009-06-26 2015-08-21 Novo Nordisk A/S Preparation containing insulin, nicotinamide and arginine
ES2550761T3 (en) 2009-11-25 2015-11-12 Novo Nordisk A/S Method for polypeptide production
CN102947331B (en) 2010-06-23 2016-08-03 诺沃—诺迪斯克有限公司 Comprise the insulin analog of extra disulfide bond
CN102933599A (en) 2010-06-23 2013-02-13 诺沃—诺迪斯克有限公司 Human insulin containing additional disulfide bonds
CN104693302A (en) 2010-06-23 2015-06-10 诺沃—诺迪斯克有限公司 Insulin derivatives containing additional disulfide bonds
EP2651432A1 (en) 2010-12-14 2013-10-23 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
CA2821613A1 (en) 2010-12-14 2012-06-21 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
EP2720711A1 (en) 2011-06-15 2014-04-23 Novo Nordisk A/S Multi substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
EP2925345B1 (en) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
US11208452B2 (en) 2015-06-02 2021-12-28 Novo Nordisk A/S Insulins with polar recombinant extensions
CN108026156A (en) 2015-08-25 2018-05-11 诺和诺德股份有限公司 Novel insulin derivates and its medical usage
CN108368163A (en) 2015-08-25 2018-08-03 诺和诺德股份有限公司 Novel insulin derivates and its medical usage
WO2017032798A1 (en) 2015-08-25 2017-03-02 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
MX2018014023A (en) * 2016-05-24 2019-04-04 Novo Nordisk As Mic-1 compounds and use thereof.
WO2019034726A1 (en) 2017-08-17 2019-02-21 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
JP2021532730A (en) * 2018-06-18 2021-12-02 ユニケム ラボラトリーズ リミテッド Leader sequence for increasing expression levels of recombinant proteins
MX2022006251A (en) 2019-12-11 2022-06-22 Novo Nordisk As Novel insulin analogues and uses thereof.
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
EP0765395A1 (en) 1997-04-02
NO965411L (en) 1997-02-14
HUT75840A (en) 1997-05-28
MX9606536A (en) 1997-03-29
FI965030A0 (en) 1996-12-16
IL114160A (en) 2006-12-31
CN1152942A (en) 1997-06-25
NO965411D0 (en) 1996-12-16
PL317761A1 (en) 1997-04-28
JPH10501695A (en) 1998-02-17
FI965030A (en) 1997-02-14
IL114160A0 (en) 1995-10-31
CZ363796A3 (en) 1997-05-14
CN1131312C (en) 2003-12-17
HU9603476D0 (en) 1997-02-28
SG47883A1 (en) 1998-04-17
AU2733595A (en) 1996-01-15
BR9508053A (en) 1997-08-12
CA2192943A1 (en) 1995-12-28
WO1995035384A1 (en) 1995-12-28
JP3730255B2 (en) 2005-12-21

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