WO2022057200A1

WO2022057200A1 - Two-step multiplex immunochromatographic detection fastener

Info

Publication number: WO2022057200A1
Application number: PCT/CN2021/076005
Authority: WO
Inventors: 付辉; 马涛; 黄永健; 曾楚怡; 覃冬梅; 杨明杰; 刘伟; 张远霞; 刘家烨; 彭柳淇; 陈柏全; 顾静燕; 范海艳; 吴文慧; 梁家源
Original assignee: 深圳市易瑞生物技术股份有限公司
Priority date: 2020-09-16
Filing date: 2021-02-08
Publication date: 2022-03-24
Also published as: CN212845412U

Abstract

A two-step multiplex immunochromatographic detection fastener, comprising a first fastener body (100) and a second fastener body (200) that are fasteningly connected to one another, and test strips (300). The first fastener body (100) is used to fix two or more test strips (300). One end of each test strip (300) in the length direction is provided with a sample pad (310) and is provided extending out of the first fastener body (100). The second fastener body (200) is used to cover the sample pad (310). By being provided with the plurality of test strips (300), the detection fastener enables the two-step immunochromatographic test strips to achieve multiplex detection. Moreover, by means of the configuration of the first fastener body (100) and the second fastener body (200), the test strips (300) may be exposed when in use, and may make contact with a solution in a gold cup to thereby achieve multiplex detection. When not in use, the test strips (300) are covered and protected by the second fastener body (200), thus preventing the test strips (300) from being damaged and contaminated.

Description

A two-step multiplex immunochromatography detection clip

technical field

The utility model relates to the technical field of food detection, in particular to a two-step multiplex immunochromatographic detection clip.

Background technique

Immunochromatographic technology has recently become a fast clinical detection technology in the medical community at home and abroad.

In the rapid detection of food safety, in order to improve the detection efficiency, the simultaneous detection of multiple targets has become the general trend. Multiple test strips are used in parallel, which can realize the simultaneous detection of multiple different items. However, the current common methods are generally based on the one-step method, that is, based on the gold pad method, which cannot be compatible with the two-step method using the gold cup, and the detection workload is large and the detection efficiency is low.

technical solutions

In order to solve the deficiencies of the prior art, the utility model provides a two-step method multiplex immunochromatography detection clip, so that the two-step method immunochromatography test strip can realize multiplex detection, and at the same time The design makes the test strip protected from the influence of the external environment.

The technical effect to be achieved by the utility model is achieved by the following scheme: a two-step multiplex immunochromatography detection buckle, comprising a first buckle body, a second buckle body and a test strip that are buckled with each other, the first buckle body The button body is used to fix more than two test strips, one end of the test strip in the length direction is provided with a sample pad and is arranged to extend from the first button body, and the second button body is used to cover the sample pad.

Preferably, the first button body includes an upper sheet shell and a lower sheet shell that are buckled with each other, and the side of the lower sheet shell facing the upper sheet shell is provided with two or more test strip support structures, so The test strip is correspondingly arranged on the test strip support structure and is fixed by the buckled upper shell and lower shell.

Preferably, the lower shell is further extended with a gasket corresponding to the sample pad.

Preferably, the extended end of the pad is shorter than the end of the sample pad.

Preferably, the second buckle body is in the shape of a slot cover with one end open, and the open end of the second buckle body is disposed toward the sample pad and is disposed to be buckled with the first buckle body.

Preferably, the upper shell is further provided with an inspection window, and the second buckle body is further provided with a notch at a position corresponding to the inspection window, so as to avoid the inspection window.

Preferably, an area for setting an identification code is also reserved on the outer surface of the upper shell.

Preferably, the test strip support structure is a frame-shaped structure integrally formed with the lower shell, and the interior is hollow.

Preferably, the frame-shaped structure also has protrusions extending parallel and inward.

beneficial effect

The utility model has the following advantages:

The detection buckle of the utility model enables the two-step immunochromatographic test strips to realize multiple detection by arranging a plurality of test strips, and the arrangement of the first buckle body and the second buckle body makes the test strips in use. It can be exposed when it comes into contact with the solution in the gold cup to realize multiple detection. The test strip is covered and protected by the second buckle body when not in use, which avoids damage and contamination of the test strip.

Description of drawings

Fig. 1 is the three-dimensional structural representation of a kind of two-step method multiplex immunochromatography detection buckle assembly in the utility model;

FIG. 2 is a schematic three-dimensional structure diagram of the separated state of the first buckle body and the second buckle body in a two-step multiplex immunochromatography detection buckle of the present invention;

3 is a schematic diagram of the exploded structure of a two-step multiplex immunochromatographic detection clip in the present invention.

Embodiments of the present invention

The present invention will be described in detail below with reference to the accompanying drawings and embodiments, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, and are intended to be used to explain the present invention, but should not be construed as a limitation of the present invention.

In the description of the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical" , "horizontal", "top", "bottom", "inside", "outside" and other indications of orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, only for the convenience of describing the present utility model and simplifying the description , rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation on the present invention.

Furthermore, the terms "first", "second" and "third" are used for descriptive purposes only, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first", "second", "third" may expressly or implicitly include one or more of that feature. In the description of the present invention, "plurality" means two or more, unless otherwise expressly and specifically defined.

In the present utility model, unless otherwise expressly specified and limited, terms such as "installation", "connection", "connection", "fixation" and "arrangement" should be understood in a broad sense, for example, it may be a fixed connection, or It can be a detachable connection or an integral body; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can also be the internal communication of the two elements or the interaction of the two elements relation. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

With reference to FIGS. 1 to 3 , an embodiment of the present invention provides a two-step multiplex immunochromatography detection buckle, including a first buckle body 100 , a second buckle body 200 and a test strip 300 that are buckled with each other. , the first button body 100 is used to fix more than two test strips 300 (four are shown in the figure), and one end of the test strip 300 in the length direction is provided with a sample pad 310 and extends out of the first button The body 100 is provided, and the sample pad 310 is used to extend into the gold cup to contact with the solution. The second buckle body 200 is used to cover the sample pad 310 so as to form a protective effect on the sample pad 310 . Compared with the one-step method, the application of the two-step method in the field of rapid detection of food safety is more extensive. The detection buckle of the present utility model is provided with a plurality of test strips 300, so that the two-step method of immunochromatographic test strips can realize multiple detection. , and through the setting of the first button body 100 and the second button body 200, the test strip 300 can be exposed when in use, and contact with the solution in the gold cup to realize multiple detection. When the test strip 300 is not in use It is covered and protected by the second buckle body 200 to avoid damage and contamination of the test strip 300 .

Specifically, the first buckle body 100 includes a top shell 110 and a bottom shell 120 that are mutually buckled, and the side of the bottom shell 120 facing the top shell 110 is provided with two or more test papers Strip support structure 130, the test strip 300 is correspondingly disposed on the test strip support structure 130 and fixed by the buckled upper shell 110 and lower shell 120, the test strip support structure 130 is used for protection The test strip 300 will not be collided or squeezed and deformed during use. Preferably, the number of the test strips 300 is the same as the number of the test strip support structures 130 , that is, each test strip 300 corresponds to one test strip support structure 130 . Preferably, the lower shell 120 is further extended with a gasket 140 corresponding to the sample pad 310 to prevent the sample pad 310 from being collided or deformed by extrusion. Further, the extended end of the gasket 140 may be slightly shorter than the end of the sample pad 310, which can ensure that the test strip 300 can fully contact the solution in the cup when it penetrates into the gold cup.

The test strip support structure 130 may be a frame-shaped structure integrally formed with the lower shell 120, and is hollow inside, which not only supports the test strip 300, but also facilitates the test strip 300 to absorb the solution. The frame-shaped structure may also have protrusions 140 extending parallel and inward to further strengthen the support for the test strip 300 .

Further, the outer surface of the upper shell 110 is also reserved with an area A for setting the identification code, which is used for printing or printing the identification code on the upper shell 110, and each detection buckle corresponds to an identification code, which is convenient for Tracking and tagging of information. The identification code may be a two-dimensional code or a bar code, which has the function of unique identification.

In the present invention, the upper shell 110 is also provided with an inspection window 150 for observing the T and C lines or further for observing the items corresponding to the test strips, and the inspection window 150 corresponds to the position of the test strips 300 set up. Further, the inspection window 150 is also provided with a transparent protective film to prevent the observed part from being affected by the environment and other factors.

In the present invention, specifically, the second buckle body 200 is in the shape of a slot cover with an opening 210 at one end. One end of the opening 210 of the second buckle body 200 is disposed toward the sample pad 310 and is buckled with the first buckle body 100 . A protective effect on the sample pad 310 is achieved. The second buckle body 200 is also provided with a gap 220 at the position corresponding to the inspection window 150 to avoid the inspection window 150, so that when the first buckle body 100 and the second buckle body 200 are buckled, the inspection window can also be observed 150.

The buckling structure of the upper shell 110 and the lower shell 120 and the buckling structure of the first buckle body 100 and the second buckle body 200 may adopt conventional designs in the prior art, which are not specifically limited in the present invention. Repeat.

When detection is required, a gold cup can also be embedded in the second buckle body 200, which can make the detection more integrated.

When assembling the detection clip of the present invention, taking the quadruple as an example, firstly place the test strips 300 of four different test strips on the corresponding test strip support structure 130, and then place the two-dimensional code engraved on the test strip 300. The upper shell 110 and the lower shell 120 are buckled and assembled to form a first buckle body 100 , and then the second buckle body 200 and the first buckle body 100 are buckled and assembled to form a detection buckle. Finally, the assembled detection buckle and the matching quadruple reaction cup can be sealed and stored in a dry state, preferably in an aluminum foil bag.

The utility model takes the quadruple immunochromatographic detection clip for detecting 16 kinds of drug residues in milk as an example. methicillin, colistin, albendazole, sulfonamides, neomycin, kanamycin, spectinomycin, gentamicin, erythromycin, lincomycin, theleromycin/tilmicol Star, fluoroquinolone residues. The detection buckle contains four test strips, each of which has four T lines, which can detect four different drugs. When testing, first take out the quadruple reaction cup with item numbers 1-4, and add 200 to the quadruple reaction cup in turn. μL milk sample. Then put the quadruple reaction cup in a 40°C incubator, and after 3 minutes of reaction, take out the quadruple immunochromatography detection buckle printed with item numbers 1-4, take off the second buckle body 200 and put the second buckle body. 200 corresponds to insert into the quadruple reaction cuvette. After reacting again for 7 minutes, the second button body 200 was taken out from the quadruple reaction cup, the test strip 300 at the lower end of the test strip was scraped off, and the result was interpreted.

Interpretation of results can be divided into visual interpretation and instrument interpretation. The color depth of the C-line and the T-line can be observed with the naked eye through the window to interpret the results. The quadruple immunochromatography detection clip can also be placed in the supporting reader, and the reader can automatically identify the relevant information of the clip according to the two-dimensional code on the buckle, such as item number, batch, test item, etc. The buckle is scanned and the interpretation result is displayed.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the embodiments of the present invention and not to limit them. Although the embodiments of the present invention have been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should It should be understood that the technical solutions of the embodiments of the present invention can still be modified or equivalently replaced, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

A two-step method multiplex immunochromatography detection buckle, characterized in that it comprises a first buckle body, a second buckle body and a test strip that are buckled with each other, and the first buckle body is used for fixing two or more A test strip, one end of the test strip in the length direction is provided with a sample pad extending from a first button body, and the second button body is used to cover the sample pad.
A two-step multiplex immunochromatography detection buckle according to claim 1, wherein the first buckle body comprises an upper plate shell and a lower plate shell that are fastened to each other, and the lower plate shell The side of the shell facing the upper shell is provided with two or more test strip support structures, the test strips are correspondingly arranged on the test strip support structure and are fixed by the buckled upper shell and lower shell .
The two-step multiplex immunochromatography detection buckle according to claim 2, wherein the lower shell is further extended with a gasket corresponding to the sample pad.
The two-step multiplex immunochromatography detection buckle according to claim 3, wherein the extended end of the gasket is shorter than the end of the sample pad.
The two-step multiplex immunochromatography detection buckle according to claim 2, wherein the second buckle body is in the shape of a groove cover with one end open, and the open end of the second buckle body faces toward The sample pad is arranged and buckled with the first buckle body.
A two-step multiple immunochromatographic detection buckle according to claim 5, wherein the upper shell is further provided with an inspection window, and the second buckle body is further opened at a position corresponding to the inspection window Notched to avoid inspection windows.
The two-step multiplex immunochromatography detection buckle according to claim 2, wherein an area for setting an identification code is reserved on the outer surface of the upper shell.
The two-step multiplex immunochromatography detection buckle according to claim 2, wherein the test strip support structure is a frame-shaped structure integrally formed with the lower shell, and the interior is hollow.
The two-step multiplex immunochromatography detection buckle according to claim 8, wherein the frame-shaped structure also has protrusions extending parallel and inward.