Injury of kidney five in one test card
Technical field
The utility model belongs to technical field of immune assay, relates to the colloidal-gold detecting-card of kidney merit mark kidney injury molecule (kim-1), neutrophil gelatinase-associated lipocalin (NAGL), IL-18 (IL-18), microdose urine protein (MAU) and bladder chalone C (cys C).
Background technology
The AKI incidence of disease is 210,0/1,000,000 crowds, and the AKI incidence of Cavity in Critical Patients especially gerontal patient increases day by day, needs the AKI mortality ratio accepting renal replacement therapies up to 50% ~ 80%, and the whole world about has 2,000,000 people to die from AKI every year.At present, lack effective methods for the treatment of for AKI (especially acute tubular necrosis), therefore, it is the key measure improving AKI patient's prognosis that early diagnosis is also intervened in time.Find that damage markers contributes to early diagnosis AKI.Clinical at present diagnosis and Main Basis serum creatinine and urine volume are by stages carried out to AKI, but often namely change in " danger " phase of AKI " damage " before the phase both this, early diagnosis cannot be done to AKI.
Kidney injury molecule-1 (Kidney injury molecule 1, KIM-1) is a kind of transmembrane glycoprotein of Kidney anomaly, belongs to immunoglobulin gene superfamily.KIM-1 in normal liver,kidney,spleen trace expression, and expresses significantly enhancing in the proximal tubular epithelial cells of impaired rear regeneration.KIM-1 can rapid, sensitive, the damage and the rejuvenation that reflect various kidney trouble specifically, can become a kind of reliabie label detecting albuminuria, have broad application prospects clinically.
Neutrophil gelatinase-associated lipocalin also known as people's lipocalin protein, or bites a newcomer in ferritin Shi Ren lipocalin protein family.In recent years, NGAL receives much attention as a kind of new injury of kidney mark.Time early diagnosis acute kidney injury (AKI), blood, urine NGAL concentration can raise rapidly usually; 2h the most obviously (raises tens to hundred times than critical value); the traditional index such as serum creatinine (sCr), urine enzyme often will in 24 ~ 72h just rear obviously rising, and thus NGAL can be used for the early diagnosis of AKI.
Interleukin 18 is the human cell factor recently found, main induction produces IFN-γ.It is active that IL-18 has enhancing host defense, antitumor activity and proinflammatory.Because of the inevitable reaction along with inflammation of injury of kidney, therefore can cause the rising of IL-18 in blood and urine, coordinate other injury of kidney mark can determine the early stage generation of injury of kidney.
Bladder chalone C (cysC) is a kind of cystatin, be also referred to as γ-trace of albumin and γ-postglobulin, in the karyocyte being extensively present in various tissue and body fluid, a kind of low-molecular-weight, alkaline non-glycated protein, molecular weight is 13.3KD, be made up of 122 amino acid residues, can be produced by all karyocytes of body, generation rate is constant.Cystatin c in circulation is only eliminated through glomerular filtration, it is a kind of endogenous mark that glomerular filtration rate(GFR changes that reflects, and heavily absorb at proximal convoluted tubule, but by complete metabolic breakdown after heavily absorbing, do not return blood, therefore, its blood level is determined by glomerular filtration, and do not rely on any foeign element, as the impact of sex, age, diet, be a kind of desirable homology mark that glomerular filtration rate(GFR changes that reflects.
Microalbuminuria refers to and occur microalbumin in urine.Albumin is the normal protein matter in a kind of blood, but only occurs minute quantity albumin in urine in physiological conditions.Microalbuminuria reflection renal abnormality leaky protein.Microdose urine protein is assessment compromised kidneys degree: when discovery microdose urine protein is within the scope of 20mg/L-200mg/L, routine urinalysis Urine proteins be shown as feminine gender (-) or (+-), just belong to microalbuminuria, illustrate that kidney damages this time.And when in urinating, microalbumin is more than 200mg/L, routine urinalysis test urine protein positive (+) ~ (+++), just should watch out for, now prove that body has a large amount of albumin and spills, Hypoproteinemia may be there is, development of renal disease only has one step away from the irreversible phase, if cured not in time, will enter Uremic.
The utility model develops a kind of colloidal-gold detecting-card simultaneously detecting five kinds of injury of kidney marks.Collaurum method has simple and quick feature accurately, the utility model utilizes collaurum method to detect five kinds of marks in human urine, avoid conventional liquid phase chromatography, running time that immunoturbidimetry, chemoluminescence method have is long, need the shortcoming of specialized equipment, only need a step simple operations, testing result can be obtained in 10 minutes.
Utility model content
The utility model provides a kind of five in one test card simultaneously detecting injury of kidney mark.Diagnose for adjuvant clinical injury of kidney.
The utility model is by the following technical solutions:
One detects injury of kidney mark five in one test card simultaneously, is made up of detection clamp and draw-in groove two parts.
Clamp gets stuck from above, under to get stuck and five kinds of injury of kidney marker colloidal gold test strip form.Under get stuck inner side have five grooves for placing colloid gold test paper.On get stuck, have five strip view windows, there are information record label in outside, for indicating that the title of test strips, testing result illustrate and sample message.Five kinds of injury of kidney marker colloidal gold test strip are by backing, sample pad, nitrocellulose filter and adsorptive pads four part composition.Adsorptive pads and sample pad lay respectively at two sections of backing, nitrocellulose filter in the middle part of backing one section with adsorptive pads is overlapping links together, the other end and sample pad is overlapping links together.Nitrocellulose filter is coated with detection line and nature controlling line.Five kinds of injury of kidney marker colloidal gold test strip are put into down in five grooves got stuck respectively, and a sample pad part for five kinds of test strips is exposed and got stuck, nitrocellulose filter and view window one_to_one corresponding.
The groove body of draw-in groove has five holes, respectively corresponding five kinds of colloidal gold strips, and the antibody of colloid gold label is contained in the bottom in hole, corresponding with five kinds of marks respectively.
The utility model colloidal gold strip used all adopts double antibody sandwich method principle to detect corresponding mark, the colloidal gold labeled monoclonal antibody of draw-in groove is mouse injury of kidney mark monoclonal antibody and colloidal gold composite, on nitrocellulose filter, detection line bag is by the mouse injury of kidney mark monoclonal antibody with antibody conjugates on pad, and nature controlling line bag is by sheep anti-mouse igg polyclonal antibody.
During detection, urine specimen is added drop-wise in the sample well of draw-in groove, and the collaurum in sample well is dissolved in sample, then clamp and draw-in groove is covered tightly, and under capillarity, urine is to adsorptive pads direction chromatography.If containing corresponding injury of kidney mark in urine, mark in sample is combined with corresponding golden labeling antibody, then on chromatography to nitrocellulose filter during detection line, be combined with the antibody of bag quilt, form the sandwich structure of " coated antibody-antigen-Jin labeling antibody compound ", and there is red stripes in detection line region, obtain positive findings like this.
When not containing injury of kidney mark in sample, sample and golden labeling antibody and coated antibody all can not react, the gold grain of redness just can not capture by tested survey line when on chromatography to nitrocellulose filter, thus can not demonstrate red stripes in this region, obtains negative findings like this.
Golden labeling antibody on the negative or golden pad of positive sample all can produce red stripes with the many anti-bindings of the sheep anti mouse of nature controlling line on nitrocellulose filter, if explicit declaration testing result is not invalid for nature controlling line.
Adopt the injury of kidney mark five in one test card of such scheme to have following beneficial effect, comprise that cost is low, compact volume and convenience, simple to operate, can detect without the need to professional, and a step can detect five kinds of injury of kidney marks simultaneously.
Accompanying drawing explanation
Fig. 1 front view of the present utility model
Fig. 2 cut-open view of the present utility model
The cut-open view of Fig. 3 injury of kidney marker detection test strips
The cut-open view of Fig. 4 draw-in groove
1. 3. sample well 4. information recording papers 5. that get stuck for 2. times of getting stuck on detect explanation 6. test paper title 7. result display window 8. test strips 9. backing 10. adsorptive pads 11. nitrocellulose filter 12. colloidal gold labeled monoclonal antibody compound 13. sample pad 14. detection line 15. nature controlling line 16. groove 21. clamp 22. draw-in groove
Embodiment
As depicted in figs. 1 and 2, the utility model is by detecting clamp 21 and draw-in groove 22 forms.Clamp gets stuck 1 from above, under get stuck 2 and injury of kidney marker detection test strips 8 form.On to get stuck inside 1 and under get stuck inside 2 and can combine closely, formed firmly overall.On get stuck and to have information recording paper 4 outside 1, detect explanation 5, test paper title 6, result display window 7.Detect explanation 5 for illustration of interpretation method, test paper title 6 is for illustration of the kind of Test paper, and result display window 7 is corresponding with the nitrocellulose filter 11 of the test strips 8 in groove 16.Under get stuck and 2 have 5 grooves 16, for placing injury of kidney marker detection test strips 8, test strips 8 to put order identical with the order of test paper title 6.
As Fig. 3, colloid gold test paper is made up of backing 9, adsorptive pads 10, nitrocellulose filter 11, sample pad 13, adsorptive pads 10 and sample pad 13 lay respectively at two sections of backing 9, nitrocellulose filter 11 in the middle part of backing 9 one section with adsorptive pads 10 is overlapping links together, the other end and sample pad 13 is overlapping links together.Nitrocellulose filter 11 is coated with detection line 14 and nature controlling line 15.The detection line of five kinds of Test papers wraps respectively by kim-1, NAGL, IL-18, MAU and cys C antibody, and nature controlling line bag is resisted by sheep anti-mouse igg more, and golden pad wraps respectively is marked kim-1, NAGL, IL-18, MAU and cys C monoclonal antibody by gold.
As Fig. 4, draw-in groove 22 is containing five sample wells 3, and the bottom of sample well 3 is placed with the antibody complex 12 of colloid gold label, the kind of antibody and injury of kidney marker detection test strips 8 one_to_one corresponding.