WO2015153791A1 - Crispr/cas-related methods and compositions for treating herpes simplex virus type 2 (hsv-2) - Google Patents

Abstract

CRISPR/CAS-related compositions and methods for treatment of HSV-2 are disclosed.

Description

CRISPR/CAS-RELATED METHODS AND COMPOSITIONS FOR TREATING

HERPES SIMPLEX VIRUS TYPE 2 (HSV-2)

REFERENCE TO RELATED APPLICATIONS

The present application claims the benefit of U.S. Provisional Application No.

61/973,792, filed April 1, 2014, the contents of which are hereby incorporated by reference in their entirety.

FIELD OF THE INVENTION

The invention relates to CRISPR/CAS-related methods and components for editing of a target nucleic acid sequence, or modulating expression of a target nucleic acid sequence, and applications thereof in connection with herpes simplex virus type 2 (HSV-2).

BACKGROUND

Herpes simplex virus type 2 (HSV-2) is a sexually transmitted virus. It is most commonly known as genital herpes. Initial infection with HSV-2 generally causes painful blistering in the genital region. The disease causes lifelong, recurring bouts of viral reactivity. It is highly contagious and increases the risk of acquiring HIV infection, especially among patients with active lesions.

More than 500 million people are infected with HSV-2 worldwide. Up to 23 million individuals are infected for the first time each year. In the United States, approximately 1 in 5 adults is seropositive for HSV-2 infection (Xu et al., Abstract 739 in 42nd Annual Meeting of the Infectious Diseases Society of America; September 30, 2004). The incidence of HSV- 2 is increasing: since 1976, there has been a 30% increase in the presence of HSV-2 seropositivity in adults in the United States (Fleming et al., New England Journal of Medicine 1997; 337:1105-11).

Initial infection with HSV-2 generally causes painful blistering of the mucous membranes of the ano-genital region, or, less commonly, the oral region. After initial infection, HSV-2 establishes latent infection in all subjects.

Infection of HSV-2 is permanent. Latent infection that lasts for the lifetime of the host. Following establishment of latent infection, reactivation of HSV-2 can occur at any point during the lifetime of the subject. Reactivation of HSV-2 is more likely to occur in the elderly and in immunocompromised individuals, including in those who have cancer, those

l who have HIV/AIDs and in those who have undergone solid organ or hematopoietic stem cell transplant.

HSV-2 encephalitis and HSV-2 meningitis are among the most severe and debilitating types of HSV infections. HSV encephalitis is the most common form of non-epidemic encephalitis. The annual incidence of HSV encephalitis is 0.2-0.4 in 100,000 individuals (Saba et al., 2012; British Medical Journal 344: e3166). Subjects who develop HSV-2 encephalitis and/or meningitis commonly have permanent neurologic sequelae.

HSV keratitis is the most common cause of infectious blindness in the developed world (Dawson et. al., Suvey of Ophthalmology 1976; 21(2): 121-135). Worldwide, there are approximately 1.5 million cases of HSV-related ophthalmologic disease and 40,000 cases of HSV-related blindness or severe monocular visual impairment annually (Krawczyk et. al., Public Library of Science One 2015; 10(1): eOl 16800. Farooq and Shukla 2012; Survey of Ophthalmology 57(5): 448-462).

Newborns are a population at particular risk for developing severe HSV-2 infections. The disease is transmitted from the mother to the fetus during childbirth. The chance of maternal-fetal transmission is highest in cases where the mother developed primary HSV infection during pregnancy and in cases where the mother has active sores in the genital region as the fetus travels through the birth canal. The incidence of neonatal herpes is approximately 4-30 per 100,000 births (Brown ZA, et al., 2003; Journal of the American Medical Association; 289(2): 203-209. Dinh T-H, et al., 2008; Sexually Transmitted Disease; 35(1): 19-21.) Neonates can develop severe HSV-2 keratitis, retinitis, encephalitis and/or meningitis. Neonatal ocular herpes (keratitis or retinitis) can result in immediate, permanent vision loss. Ocular HSV-2 puts neonates at risk for later developing HSV-2 associated acute retinal necrosis, which can also lead to permanent vision loss. Untreated HSV-2 encephalitis leads to death in 50% of neonates. Even with prompt treatment with antiviral therapy, the majority of neonates who contract HSV-2 encephalitis or meningitis will suffer from permanent neurologic sequelae.

There are no curative or preventative treatments. Therapy is primarily given during acute infection. Primary HSV-2 infections can be is treated with antiviral therapy, including acyclovir, valacyclovir and famciclovir. These therapies may reduce viral shedding, decrease pain and improve healing time of lesions. Re-activated, latent infections may resolve without treatment (may be self-limiting) or may be treated with anti-viral therapy. Antiviral therapy may be given prophylactically in certain situations, including during childbirth in a mother with a recent HSV-2 infection or reactivation. Vaccines are in development for the prevention of HSV-2 infection. However, in controlled clinical trials, vaccination efficacy has been limited. A recent vaccine for both HS V- 1 and HSV-2 infections was found to be ineffective in the prevention of HSV-2 genital infections (Belshe et al., 2012; New England Journal of Medicine 366(1): 34-43).

Despite advances in antiretro viral therapies, there remains a need for the treatment and prevention of HSV-2 infection, particularly the treatment and prevention of HSV-2 associated keratitis, encephalitis and meningitis. A therapy that can cure, prevent, or treat HSV-2 infections would be superior to the current standard of care. SUMMARY OF THE INVENTION

Methods and compositions discussed herein, provide for the treatment or prevention of herpes simplex virus type 2 (HSV-2), commonly known as genital herpes. HSV-2 is contained within an icosahedral particle. The virus enters the host via infection of epithelial cells within the skin and mucous membranes. The virus produces immediate early genes within the epithelial cells, which encode enzymes and binding proteins necessary for viral synthesis. After primary infection, the virus travels up sensory nerve axons via retrograde transport to the sensory dorsal root ganglion (DRG). HSV-2 mainly travels to the sensory DRG located within the sacrum, but can travel to other sensory ganglia depending upon the site of primary infection. Within the DRG, it establishes a latent infection. The latent infection persists for the lifetime of the host. Within the DRG cell the virus uncoats, viral

DNA is transported into the nucleus of the sensory nerve, and key viral RNAs associated with latency are transcribed (i.e., the LAT RNAs).

Methods and compositions discussed herein can be used to provide for treatment or prevention of herpes simplex virus type 2 (HSV-2), or its symptoms, e.g., by knocking out one or more of the HSV-2 viral genes, e.g., by knocking out one or more of UL19, UL30, UL48 and/or UL54 gene(s). In one aspect, methods and compositions discussed herein may be used to alter one or more of the UL19, UL30, UL48 and/or UL54 gene(s) to treat or prevent HSV-2, by targeting, the gene, e.g., the non-coding or coding regions, e.g., the promoter region, or a transcribed sequence, e.g., intronic or exonic sequence. In an embodiment coding sequence, e.g., a coding region, e.g., an early coding region, of one or more the UL19, UL30, UL48 and/or UL54 gene(s), is targeted for alteration and knockout of expression.

In another aspect, the methods and compositions discussed herein may be used to alter one or more of the UL19, UL30, UL48 and/or UL54 gene(s) to treat or prevent herpes simplex virus type 2 (HSV-2) by targeting the coding sequence of one or more of the UL19, UL30, UL48 and/or UL54 gene(s). In one embodiment, the gene, e.g., the coding sequence of one or more of the UL19, UL30, UL48 and/or UL54 gene(s), are targeted to knockout one or more of UL19, UL30, UL48 and/or UL54 gene(s), e.g., to eliminate expression of one or more of UL19, UL30, UL48 and/or UL54 gene(s), e.g., to knockout one or more copies of one or more of UL19, UL30, UL48 and/or UL54 gene(s), e.g., by induction of an alteration comprising a deletion or mutation in one or more of UL19, UL30, UL48 and/or UL54 gene(s). In an embodiment, the method provides an alteration that comprises an insertion or deletion. As described herein, a targeted knockout approach is mediated by non-homologous end joining (NHEJ) using a CRISPR/Cas system comprising an enzymatically active Cas9 (eaCas9) molecule.

In one embodiment, an early coding sequence of one or more of the UL19, UL30, UL48 and/or UL54 gene(s) is targeted to knockout one or more of UL19, UL30, UL48 and/or UL54 gene(s). In an embodiment, targeting affects one or more copies of the UL19, UL30, UL48 and/or UL54 gene(s). In an embodiment, a targeted knockout approach reduces or eliminates expression of one or more functional UL19, UL30, UL48 and/or UL54 gene product(s). In an embodiment, the method provides an alteration that comprises an insertion or deletion.

In another aspect, the methods and compositions discussed herein may be used to alter one or more of the UL19, UL30, UL48 and/or UL54 gene(s) to treat or prevent HSV-2 by targeting non-coding sequence of the UL19, UL30, UL48 and/or UL54 gene(s), e.g., promoter, an enhancer, an intron, 3'UTR, and/or polyadenylation signal. In one embodiment, the gene, e.g., the non-coding sequence of the UL19, UL30, UL48 and/or UL54 gene(s), is targeted to knockout the gene, e.g., to eliminate expression of the gene, e.g., to knockout one or more copies of the UL19, UL30, UL48 and/or UL54 gene(s), e.g., by induction of an alteration comprising a deletion or mutation in the UL19, UL30, UL48 and/or UL54 gene(s). In an embodiment, the method provides an alteration that comprises an insertion or deletion.

"HSV-2 target UL19 position", as used herein, refers to a position in the UL19 gene, which if altered by NHEJ-mediated alteration, results in reduction or elimination of expression of functional UL19 gene product. In an embodiment, the position is in the UL19 gene coding region, e.g., an early coding region.

"HSV-2 target UL30 position", as used herein, refers to a position in the UL30 gene, which if altered by NHEJ-mediated alteration, results in reduction or elimination of expression of functional UL30 gene product. In an embodiment, the position is in the UL30 gene coding region, e.g., an early coding region.

"HSV-2 target UL48 position", as used herein, refers to a position in the UL48 gene, which if altered by NHEJ-mediated alteration, results in reduction or elimination of expression of functional UL48 gene product. In an embodiment, the position is in the UL48 gene coding region, e.g., an early coding region.

"HSV-2 target UL54 position", as used herein, refers to a position in the UL54 gene, which if altered by NHEJ-mediated alteration, results in reduction or elimination of expression of functional UL54 gene product. In an embodiment, the position is in the UL54 gene coding region, e.g., an early coding region.

"HSV-2 target position", as used herein, refers to any of a HSV-2 target UL19 target position, a HSV-2 target UL30 target position, a HSV-2 target UL48 target position or a HSV-2 target UL54 target position.

In one aspect, disclosed herein is a gRNA molecule, e.g., an isolated or non- naturally occurring gRNA molecule, comprising a targeting domain which is complementary with a target domain from the UL19, UL30, UL48 or UL54 gene.

In an embodiment, the targeting domain of the gRNA molecule is configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene to allow alteration, e.g., alteration associated with NHEJ, of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene. In an embodiment, the targeting domain is configured such that a cleavage event, e.g., a double strand or single strand break, is positioned within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of a HSV-2 target position. The break, e.g., a double strand or single strand break, can be positioned upstream or downstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene. In an embodiment, the targeting domain of the gRNA molecule is configured to provide a cleavage event selected from a double strand break and a single strand break, within 500 (e.g., within 500, 400, 300, 250, 200, 150, 100, 80, 60, 40, 20, or 10) nucleotides of a HSV-2 target position.

In an embodiment, a second gRNA molecule comprising a second targeting domain is configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to the HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, to allow alteration, e.g., alteration associated with NHEJ, of the HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, either alone or in combination with the break positioned by said first gRNA molecule. In an embodiment, the targeting domains of the first and second gRNA molecules are configured such that a cleavage event, e.g., a double strand or single strand break, is positioned, independently for each of the gRNA molecules, within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position. In an embodiment, the breaks, e.g., double strand or single strand breaks, are positioned on both sides of a nucleotide of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene. In an embodiment, the breaks, e.g., double strand or single strand breaks, are positioned on one side, e.g., upstream or downstream, of a nucleotide of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene. In an embodiment, the targeting domain of the first and/or second gRNA molecule is configured to provide a cleavage event selected from a double strand break and a single strand break, within 500 (e.g., within 500, 400, 300, 250, 200, 150, 100, 80, 60, 40, 20, or 10) nucleotides of a HSV-2 target position.

In an embodiment, a single strand break is accompanied by an additional single strand break, positioned by a second gRNA molecule, as discussed below. For example, the targeting domains are configured such that a cleavage event, e.g., the two single strand breaks, are positioned within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of a HSV-2 target position. In an embodiment, the first and second gRNA molecules are configured such, that when guiding a Cas9 molecule, e.g., a Cas9 nickase, a single strand break will be accompanied by an additional single strand break, positioned by a second gRNA, sufficiently close to one another to result in alteration of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene. In an embodiment, the first and second gRNA molecules are configured such that a single strand break positioned by said second gRNA is within 10, 20, 30, 40, or 50 nucleotides of the break positioned by said first gRNA molecule, e.g., when the Cas9 molecule is a nickase. In an embodiment, the two gRNA molecules are configured to position cuts at the same position, or within a few nucleotides of one another, on different strands, e.g., essentially mimicking a double strand break.

In an embodiment, a double strand break can be accompanied by an additional double strand break, positioned by a second gRNA molecule, as is discussed below. For example, the targeting domain of a first gRNA molecule is configured such that a double strand break is positioned upstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position; and the targeting domain of a second gRNA molecule is configured such that a double strand break is positioned downstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position.

In an embodiment, a double strand break can be accompanied by two additional single strand breaks, positioned by a second gRNA molecule and a third gRNA molecule. For example, the targeting domain of a first gRNA molecule is configured such that a double strand break is positioned upstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position; and the targeting domains of a second and third gRNA molecule are configured such that two single strand breaks are positioned downstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position. In an embodiment, the targeting domain of the first, second and third gRNA molecules are configured such that a cleavage event, e.g., a double strand or single strand break, is positioned, independently for each of the gRNA molecules.

In an embodiment, a first and second single strand breaks can be accompanied by two additional single strand breaks positioned by a third gRNA molecule and a fourth gRNA molecule. For example, the targeting domain of a first and second gRNA molecule are configured such that two single strand breaks are positioned upstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position; and the targeting domains of a third and fourth gRNA molecule are configured such that two single strand breaks are positioned downstream of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, e.g., within 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150 or 200 nucleotides of the target position. In an embodiment, the targeting domain of the first, second, third, and/or fourth gRNA molecule is configured to provide a cleavage event selected from a double strand break and a single strand break, within 500 (e.g., within 500, 400, 300, 250, 200, 150, 100, 80, 60, 40, 20, or 10) nucleotides of a HSV-2 target position.

It is contemplated herein that, in an embodiment, when multiple gRNAs are used to generate (1) two single stranded breaks in close proximity, (2) two double stranded breaks, e.g., flanking a HSV-2 target position (e.g., to remove a piece of DNA, e.g., to create a deletion mutation) or to create more than one indel in the gene, e.g., in a coding region, e.g., an early coding region, (3) one double stranded break and two paired nicks flanking a HSV-2 target position (e.g., to remove a piece of DNA, e.g., to insert a deletion) or (4) four single stranded breaks, two on each side of a position, that they are targeting the same HSV-2 target position. It is further contemplated herein that multiple gRNAs may be used to target more than one HSV-2 target position in the same gene, e.g., one or more of UL19, UL30, UL48 and/or UL54 gene(s).

In an embodiment, the targeting domain of the first gRNA molecule and the targeting domain of the second gRNA molecules are complementary to opposite strands of the target nucleic acid molecule. In an embodiment, the gRNA molecule and the second gRNA molecule are configured such that the PAMs are oriented outward.

In an embodiment, the targeting domain of a gRNA molecule is configured to avoid unwanted target chromosome elements, such as repeat elements, e.g., Alu repeats, in the target domain. The gRNA molecule may be a first, second, third and/or fourth gRNA molecule, as described herein.

In an embodiment, the targeting domain of a gRNA molecule is configured to position a cleavage event sufficiently far from a preselected nucleotide, e.g., the nucleotide of a coding region, such that the nucleotide is not altered. In an embodiment, the targeting domain of a gRNA molecule is configured to position an intronic cleavage event sufficiently far from an intron/exon border, or naturally occurring splice signal, to avoid alteration of the exonic sequence or unwanted splicing events. The gRNA molecule may be a first, second, third and/or fourth gRNA molecule, as described herein.

In an embodiment, the targeting domain comprises a sequence that is the same as, or differs by no more than 3 nucleotides from, a targeting domain sequence described herein, e.g., from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A- 9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C. In an embodiment, the targeting domain comprises a sequence that is the same as a targeting domain sequence described herein, e.g., from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

In another embodiment, a HSV-2 target position in the coding region, e.g., the early coding region, of the UL19, UL30, UL48 or UL54 gene is targeted, e.g., for knockout. In an embodiment, the targeting domain comprises a sequence that is the same as, or differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from any one of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, or Tables 4A-4G. In an embodiment, the targeting domain is independently selected from those in Tables 1A-1G. In another embodiment, the targeting domain is independently selected from Table 1A. In an embodiment, the targeting domain is independently selected from those in Tables 2A-2F. In an embodiment, the targeting domain is independently selected from Table 2A.

In an embodiment, the targeting domain is independently selected from those in Tables 3A-3F. In an embodiment, the targeting domain is independently selected from Table 3A.

In an embodiment, the targeting domain is independently selected from those in Tables 4A-4G. In an embodiment, the targeting domain is independently selected from Table 4A.

In an embodiment, the targeting domain is independently selected from those in

Tables 5A-5E. In an embodiment, the targeting domain is independently selected from Table 5A.

In an embodiment, the targeting domain is independently selected from those in Tables 6A-6G. In an embodiment, the targeting domain is independently selected from Table 6A.

In an embodiment, the targeting domain is independently selected from those in Tables 7A-7D. In an embodiment, the targeting domain is independently selected from Table 7A.

In an embodiment, the targeting domain is independently selected from those in Tables 8A-8E. In an embodiment, the targeting domain is independently selected from Table 8A.

In an embodiment, the targeting domain is independently selected from those in Tables 9A-9G. In an embodiment, the targeting domain is independently selected from Table 9A.

In an embodiment, the targeting domain is independently selected from those in

Tables 10A-10D. In an embodiment, the targeting domain is independently selected from Table 10A.

In an embodiment, the targeting domain is independently selected from those in Tables 11A-11E. In an embodiment, the targeting domain is independently selected from Table 11A.

In an embodiment, the targeting domain is independently selected from those in Tables 12A-12G. In an embodiment, the targeting domain is independently selected from Table 12A. In an embodiment, the targeting domain is independently selected from those in

Tables 13A-13B. In an embodiment, the targeting domain is independently selected from Table 13A.

In an embodiment, the targeting domain is independently selected from those in

Tables 14A-14E. In an embodiment, the targeting domain is independently selected from Table 14A.

In an embodiment, the targeting domain is independently selected from those in

Tables 15A-15G. In an embodiment, the targeting domain is independently selected from Table 15A.

In an embodiment, the targeting domain is independently selected from those in

Tables 16A-16C. In an embodiment, the targeting domain is independently selected from Table 16A.

In an embodiment, when the HSV-2 target position is the UL19, UL30, UL48 or UL54 gene coding region, e.g., an early coding region, and more than one gRNA is used to position breaks, e.g., two single stranded breaks or two double stranded breaks, or a combination of single strand and double strand breaks, e.g., to create one or more indels, in the target nucleic acid sequence, the targeting domain of each guide RNA is independently selected from one of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F or Tables 4A-4G.

In an embodiment, when the HSV-2 target position is the UL19 gene coding region, e.g., an early coding region, and more than one gRNA is used to position breaks, e.g., two single stranded breaks or two double stranded breaks, or a combination of single strand and double strand breaks, e.g., to create one or more indels, in the target nucleic acid sequence, the targeting domain of each guide RNA is independently selected from any one of Tables 1A-1G, 5A-5E, 6A-6G, or 7A-7D.

In an embodiment, when the HSV-2 target position is the UL30 gene coding region, e.g., an early coding region, and more than one gRNA is used to position breaks, e.g., two single stranded breaks or two double stranded breaks, or a combination of single strand and double strand breaks, e.g., to create one or more indels, in the target nucleic acid sequence, the targeting domain of each guide RNA is independently selected from one of Tables 2A- 2F, 8A-8E, 9A-9G, or 10A-10D.

In an embodiment, when the HSV-2 target position is the UL48 gene coding region, e.g., an early coding region, and more than one gRNA is used to position breaks, e.g., two single stranded breaks or two double stranded breaks, or a combination of single strand and double strand breaks, e.g., to create one or more indels, in the target nucleic acid sequence, the targeting domain of each guide RNA is independently selected from one of Tables 3A- 3F, 11A-11E, 12A-12G, or 13A-13B.

In an embodiment, when the HSV-2 target position is the UL54 gene coding region, e.g., an early coding region, and more than one gRNA is used to position breaks, e.g., two single stranded breaks or two double stranded breaks, or a combination of single strand and double strand breaks, e.g., to create one or more indels, in the target nucleic acid sequence, the targeting domain of each guide RNA is independently selected from one of Tables 4A- 4G, 14A-14E, 15A-15G, or 16A-16C.

In an embodiment, the gRNA, e.g., a gRNA comprising a targeting domain, which is complementary with the UL19, UL30, UL48 or UL54 gene, is a modular gRNA. In other embodiments, the gRNA is a unimolecular or chimeric gRNA.

In an embodiment, the targeting domain which is complementary with a target domain from the HSV-2 target position in the UL19, UL30, UL48 or UL54 gene is 16 nucleotides or more in length. In an embodiment, the targeting domain is 16 nucleotides in length. In an embodiment, the targeting domain is 17 nucleotides in length. In another embodiment, the targeting domain is 18 nucleotides in length. In still another embodiment, the targeting domain is 19 nucleotides in length. In still another embodiments, the targeting domain is 20 nucleotides in length. In still another embodiment, the targeting domain is 21 nucleotides in length. In still another embodiment, the targeting domain is 22 nucleotides in length. In still another embodiment, the targeting domain is 23 nucleotides in length. In still another embodiment, the targeting domain is 24 nucleotides in length. In still another embodiment, the targeting domain is 25 nucleotides in length. In still another embodiment, the targeting domain is 26 nucleotides in length.

In an embodiment, the targeting domain comprises 16 nucleotides.

In an embodiment, the targeting domain comprises 17 nucleotides.

In an embodiment, the targeting domain comprises 18 nucleotides.

In an embodiment, the targeting domain comprises 19 nucleotides.

In an embodiment, the targeting domain comprises 20 nucleotides.

In an embodiment, the targeting domain comprises 21 nucleotides.

In an embodiment, the targeting domain comprises 22 nucleotides.

In an embodiment, the targeting domain comprises 23 nucleotides.

In an embodiment, the targeting domain comprises 24 nucleotides.

In an embodiment, the targeting domain comprises 25 nucleotides.

In an embodiment, the targeting domain comprises 26 nucleotides. A gRNA as described herein may comprise from 5' to 3': a targeting domain (comprising a "core domain", and optionally a "secondary domain"); a first complementarity domain; a linking domain; a second complementarity domain; a proximal domain; and a tail domain. In some embodiments, the proximal domain and tail domain are taken together as a single domain.

In an embodiment, a gRNA comprises a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and a targeting domain equal to or greather than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26nucleotides in length.

In another embodiment, a gRNA comprises a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and a targeting domain equal to or greather than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26nucleotides in length.

In another embodiment, a gRNA comprises a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 35 nucleotides in length; and a targeting domain equal to or greather than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26nucleotides in length.

In another embodiment, a gRNA comprises a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and a targeting domain equal to or greather than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26nucleotides in length.

A cleavage event, e.g., a double strand or single strand break, is generated by a Cas9 molecule. The Cas9 molecule may be an enzymatically active Cas9 (eaCas9) molecule, e.g., an eaCas9 molecule that forms a double strand break in a target nucleic acid or an eaCas9 molecule forms a single strand break in a target nucleic acid (e.g., a nickase molecule).

In an embodiment, the eaCas9 molecule catalyzes a double strand break.

In some embodiments, the eaCas9 molecule comprises HNH-like domain cleavage activity but has no, or no significant, N-terminal RuvC-like domain cleavage activity. In this case, the eaCas9 molecule is an HNH-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at D10, e.g., D10A. In other embodiments, the eaCas9 molecule comprises N-terminal RuvC-like domain cleavage activity but has no, or no significant, HNH-like domain cleavage activity. In an embodiment, the eaCas9 molecule is an N- terminal RuvC-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at H840, e.g., H840A. In an embodiment, the eaCas9 molecule is an N-terminal RuvC-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at H863, e.g., H863A.

In an embodiment, a single strand break is formed in the strand of the target nucleic acid to which the targeting domain of said gRNA is complementary. In another embodiment, a single strand break is formed in the strand of the target nucleic acid other than the strand to which the targeting domain of said gRNA is complementary.

In another aspect, disclosed herein is a nucleic acid, e.g., an isolated or non-naturally occurring nucleic acid, e.g., DNA, that comprises (a) a sequence that encodes a gRNA molecule comprising a targeting domain that is complementary with a target domain, e.g., with a HSV-2 target position in UL19, UL30, UL48 or UL54 gene as disclosed herein.

In an embodiment, the nucleic acid encodes a gRNA molecule, e.g., a first gRNA molecule, comprising a targeting domain configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene to allow alteration, e.g., alteration associated with NHEJ, of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene.

In an embodiment, the nucleic acid encodes a gRNA molecule, e.g., the first gRNA molecule, comprising a targeting domain comprising a sequence that is the same as, or differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from any one of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A- 6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A- 16C. In an embodiment, the nucleic acid encodes a gRNA molecule comprising a targeting domain is selected from those in Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A- 4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C.

In an embodiment, the nucleic acid encodes a modular gRNA, e.g., one or more nucleic acids encode a modular gRNA. In other embodiments, the nucleic acid encodes a chimeric gRNA. The nucleic acid may encode a gRNA, e.g., the first gRNA molecule, comprising a targeting domain comprising 16 nucleotides or more in length. In an embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 16 nucleotides in length. In another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 17 nucleotides in length. In another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 18 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 19 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 20 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 21 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 22 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 23 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 24 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 25 nucleotides in length. In still another embodiment, the nucleic acid encodes a gRNA, e.g., the first gRNA molecule, comprising a targeting domain that is 26 nucleotides in length.

In an embodiment, the targeting domain comprises 16 nucleotides.

In an embodiment, the targeting domain comprises 17 nucleotides.

In an embodiment, the targeting domain comprises 18 nucleotides.

In an embodiment, the targeting domain comprises 19 nucleotides.

In an embodiment, the targeting domain comprises 20 nucleotides.

In an embodiment, the targeting domain comprises 21 nucleotides.

In an embodiment, the targeting domain comprises 22 nucleotides.

In an embodiment, the targeting domain comprises 23 nucleotides.

In an embodiment, the targeting domain comprises 24 nucleotides.

In an embodiment, the targeting domain comprises 25 nucleotides.

In an embodiment, the targeting domain comprises 26 nucleotides.

In an embodiment, a nucleic acid encodes a gRNA comprising from 5' to 3': a targeting domain (comprising a "core domain", and optionally a "secondary domain"); a first complementarity domain; a linking domain; a second complementarity domain; a proximal domain; and a tail domain. In some embodiments, the proximal domain and tail domain are taken together as a single domain.

In an embodiment, a nucleic acid encodes a gRNA e.g., the first gRNA molecule, comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and a targeting equal to or greater than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a gRNA e.g., the first gRNA molecule, comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and a targeting domain equal to or greater than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a gRNA e.g., the first gRNA molecule, comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 35 nucleotides in length; and a targeting domain equal to or greater than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a gRNA comprising e.g., the first gRNA molecule, a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and a targeting domain equal to or greater than 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid comprises (a) a sequence that encodes a gRNA molecule, e.g., the first gRNA molecule, comprising a targeting domain that is

complementary with a target domain in the UL19, UL30, UL48 or UL54 gene as disclosed herein, and further comprising (b) a sequence that encodes a Cas9 molecule.

The Cas9 molecule may be a nickase molecule, an enzymatically activating Cas9 (eaCas9) molecule, e.g., an eaCas9 molecule that forms a double strand break in a target nucleic acid and/or an eaCas9 molecule that forms a single strand break in a target nucleic acid. In an embodiment, a single strand break is formed in the strand of the target nucleic acid to which the targeting domain of said gRNA is complementary. In another embodiment, a single strand break is formed in the strand of the target nucleic acid other than the strand to which to which the targeting domain of said gRNA is complementary.

In an embodiment, the eaCas9 molecule catalyzes a double strand break.

In an embodiment, the eaCas9 molecule comprises HNH-like domain cleavage activity but has no, or no significant, N-terminal RuvC-like domain cleavage activity. In another embodiment, the said eaCas9 molecule is an HNH-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at D10, e.g., D10A. In another embodiment, the eaCas9 molecule comprises N-terminal RuvC-like domain cleavage activity but has no, or no significant, HNH-like domain cleavage activity. In another embodiment, the eaCas9 molecule is an N-terminal RuvC-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at H840, e.g., H840A. In another embodiment, the eaCas9 molecule is an N- terminal RuvC-like domain nickase, e.g., the eaCas9 molecule comprises a mutation at H863, e.g., H863A.

A nucleic acid disclosed herein may comprise (a) a sequence that encodes a gRNA molecule comprising a targeting domain that is complementary with a target domain in the CCR5 gene as disclosed herein; (b) a sequence that encodes a Cas9 molecule.

A nucleic acid disclosed herein may comprise (a) a sequence that encodes a gRNA molecule comprising a targeting domain that is complementary with a target domain in the UL19, UL30, UL48 or UL54 gene as disclosed herein; (b) a sequence that encodes a Cas9 molecule; and further may comprise (c)(i) a sequence that encodes a second gRNA molecule described herein having a targeting domain that is complementary to a second target domain of the UL19, UL30, UL48 or UL54 gene, and optionally, (c)(ii) a sequence that encodes a third gRNA molecule described herein having a targeting domain that is complementary to a third target domain of the UL19, UL30, UL48 or UL54 gene; and optionally, (c)(iii) a sequence that encodes a fourth gRNA molecule described herein having a targeting domain that is complementary to a fourth target domain of the UL19, UL30, UL48 or UL54 gene.

In an embodiment, a nucleic acid encodes a second gRNA molecule comprising a targeting domain configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, to allow alteration, e.g., alteration associated with NHEJ, of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, either alone or in combination with the break positioned by said first gRNA molecule.

In an embodiment, a nucleic acid encodes a third gRNA molecule comprising a targeting domain configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene to allow alteration, e.g., alteration associated with NHEJ, of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, either alone or in combination with the break positioned by the first and/or second gRNA molecule.

In an embodiment, a nucleic acid encodes a fourth gRNA molecule comprising a targeting domain configured to provide a cleavage event, e.g., a double strand break or a single strand break, sufficiently close to a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene to allow alteration, e.g., alteration associated with NHEJ, of a HSV-2 target position in the UL19, UL30, UL48 or UL54 gene, either alone or in combination with the break positioned by the first gRNA molecule, the second gRNA molecule and/or the third gRNA molecule. In an embodiment, the nucleic acid encodes a second gRNA molecule. The second gRNA is selected to target the same HSV-2 target position as the first gRNA molecule. Optionally, the nucleic acid may encode a third gRNA, and further optionally, the nucleic acid may encode a fourth gRNA molecule. The third gRNA molecule and the fourth gRNA molecule are selected to target the same HSV-2 target position as the first and second gRNA molecules.

In an embodiment, the nucleic acid encodes a second gRNA molecule comprising a targeting domain comprising a sequence that is the same as, or differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from one of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C. In an embodiment, the nucleic acid encodes a second gRNA molecule comprising a targeting domain selected from those in Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A- 10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A- 15G, or Tables 16A-16C. In an embodiment, when a third or fourth gRNA molecule are present, the third and fourth gRNA molecules may independently comprise a targeting domain comprising a sequence that is the same as, or differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from one of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A- 8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C. In a further embodiment, when a third or fourth gRNA molecule are present, the third and fourth gRNA molecules may independently comprise a targeting domain selected from those in Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C.

In an embodiment, the nucleic acid encodes a second gRNA which is a modular gRNA, e.g., wherein one or more nucleic acid molecules encode a modular gRNA. In another embodiment, the nucleic acid encoding a second gRNA is a chimeric gRNA. In another embodimentls, when a nucleic acid encodes a third or fourth gRNA, the third and fourth gRNA may be a modular gRNA or a chimeric gRNA. When multiple gRNAs are used, any combination of modular or chimeric gRNAs may be used. A nucleic acid may encode a second, a third, and/or a fourth gRNA, each independently, comprising a targeting domain comprising 16 nucleotides or more in length. In an embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 16 nucleotides in length. In another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 17 nucleotides in length. In another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 18 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 19 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 20 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 21 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 22 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 23 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 24 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 25 nucleotides in length. In still another embodiment, the nucleic acid encodes a second gRNA comprising a targeting domain that is 26 nucleotides in length.

In an embodiment, the targeting domain comprises 16 nucleotides.

In an embodiment, the targeting domain comprises 17 nucleotides.

In an embodiment, the targeting domain comprises 18 nucleotides.

In an embodiment, the targeting domain comprises 19 nucleotides.

In an embodiment, the targeting domain comprises 20 nucleotides.

In an embodiment, the targeting domain comprises 21 nucleotides.

In an embodiment, the targeting domain comprises 22 nucleotides.

In an embodiment, the targeting domain comprises 23 nucleotides.

In an embodiment, the targeting domain comprises 24 nucleotides.

In an embodiment, the targeting domain comprises 25 nucleotides.

In an embodiment, the targeting domain comprises 26 nucleotides.

In an embodiment, a nucleic acid encodes a second, a third, and/or a fourth gRNA, each independently, comprising from 5 ' to 3 ' : a targeting domain (comprising a "core domain", and optionally a "secondary domain"); a first complementarity domain; a linking domain; a second complementarity domain; a proximal domain; and a tail domain. In some embodiments, the proximal domain and tail domain are taken together as a single domain.

In an embodiment, a nucleic acid encodes a second, a third, and/or a fourth gRNA comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and a targeting domain equal to or greater thanl6, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a second, a third, and/or a fourth gRNA comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and a targeting domain equal to or greater thanl6, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a second, a third, and/or a fourth gRNA comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 35 nucleotides in length; and a targeting domain equal to or greater thanl6, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes a second, a third, and/or a fourth gRNA comprising a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and a targeting domain equal to or greater thanl6, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, a nucleic acid encodes (a) a sequence that encodes a gRNA molecule comprising a targeting domain that is complementary with a target domain in the UL19, UL30, UL48 or UL54 gene, as disclosed herein, and (b) a sequence that encodes a Cas9 molecule, e.g., a Cas9 molecule described herein. In an embodiment, (a) and (b) are present on the same nucleic acid molecule, e.g., the same vector, e.g., the same viral vector, e.g., the same adeno- associated virus (AAV) vector. In an embodiment, the nucleic acid molecule is an AAV vector. Exemplary AAV vectors that may be used in any of the described compositions and methods include an AAV2 vector, a modified AAV2 vector, an AAV3 vector, a modified AAV3 vector, an AAV6 vector, a modified AAV6 vector, an AAV8 vector and an AAV9 vector.

In another embodiment, (a) is present on a first nucleic acid molecule, e.g. a first vector, e.g., a first viral vector, e.g., a first AAV vector; and (b) is present on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. The first and second nucleic acid molecules may be AAV vectors.

In another embodiment, a nucleic acid encodes (a) a sequence that encodes a gRNA molecule comprising a targeting domain that is complementary with a target domain in the UL19, UL30, UL48 or UL54 gene as disclosed herein, and (b) a sequence that encodes a Cas9 molecule, e.g., a Cas9 molecule described herein; and further comprises (c)(i) a sequence that encodes a second gRNA molecule as described herein, and optionally, (c)(ii) a sequence that encodes a third gRNA molecule described herein having a targeting domain that is complementary to a third target domain of the UL19, UL30, UL48 or UL54 gene; and optionally, (c)(iii) a sequence that encodes a fourth gRNA molecule described herein having a targeting domain that is complementary to a fourth target domain of the UL19, UL30, UL48 or UL54 gene. In an embodiment, the nucleic acid comprises (a), (b) and (c)(i). In an embodiment, the nucleic acid comprises (a), (b), (c)(i) and (c)(ii). In an embodiment, the nucleic acid comprises (a), (b), (c)(i), (c)(ii) and (c)(iii). Each of (a) and (c)(i) may be present on the same nucleic acid molecule, e.g., the same vector, e.g., the same viral vector, e.g., the same adeno-associated virus (AAV) vector. In an embodiment, the nucleic acid molecule is an AAV vector.

In another embodiment, (a) and (c)(i) are on different vectors. For example, (a) may be present on a first nucleic acid molecule, e.g. a first vector, e.g., a first viral vector, e.g., a first AAV vector; and (c)(i) may be present on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. In an embodiment, the first and second nucleic acid molecules are AAV vectors.

In another embodiment, each of (a), (b), and (c)(i) are present on the same nucleic acid molecule, e.g., the same vector, e.g., the same viral vector, e.g., an AAV vector. In an embodiment, the nucleic acid molecule is an AAV vector. In an alternate embodiment, one of (a), (b), and (c)(i) is encoded on a first nucleic acid molecule, e.g., a first vector, e.g., a first viral vector, e.g., a first AAV vector; and a second and third of (a), (b), and (c)(i) is encoded on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. The first and second nucleic acid molecule may be AAV vectors.

In an embodiment, (a) is present on a first nucleic acid molecule, e.g., a first vector, e.g., a first viral vector, a first AAV vector; and (b) and (c)(i) are present on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. The first and second nucleic acid molecule may be AAV vectors.

In another embodiment, (b) is present on a first nucleic acid molecule, e.g., a first vector, e.g., a first viral vector, e.g., a first AAV vector; and (a) and (c)(i) are present on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. The first and second nucleic acid molecule may be AAV vectors. In another embodiment, (c)(i) is present on a first nucleic acid molecule, e.g., a first vector, e.g., a first viral vector, e.g., a first AAV vector; and (b) and (a) are present on a second nucleic acid molecule, e.g., a second vector, e.g., a second vector, e.g., a second AAV vector. The first and second nucleic acid molecule may be AAV vectors.

In another embodiment, each of (a), (b) and (c)(i) are present on different nucleic acid molecules, e.g., different vectors, e.g., different viral vectors, e.g., different AAV vector. For example, (a) may be on a first nucleic acid molecule, (b) on a second nucleic acid molecule, and (c)(i) on a third nucleic acid molecule. The first, second and third nucleic acid molecule may be AAV vectors.

In another embodiment, when a third and/or fourth gRNA molecule are present, each of (a), (b), (c)(i), (c)(ii) and (c)(iii) may be present on the same nucleic acid molecule, e.g., the same vector, e.g., the same viral vector, e.g., an AAV vector. In an embodiment, the nucleic acid molecule is an AAV vector. In an alternate embodiment, each of (a), (b), (c)(i), (c)(ii) and (c)(iii) may be present on the different nucleic acid molecules, e.g., different vectors, e.g., the different viral vectors, e.g., different AAV vectors. In a further

embodiment, each of (a), (b), (c)(i), (c)(ii) and (c)(iii) may be present on more than one nucleic acid molecule, but fewer than five nucleic acid molecules, e.g., AAV vectors.

The nucleic acids described herein may comprise a promoter operably linked to the sequence that encodes the gRNA molecule of (a), e.g., a promoter described herein. The nucleic acid may further comprise a second promoter operably linked to the sequence that encodes the second, third and/or fourth gRNA molecule of (c), e.g., a promoter described herein. The promoter and second promoter differ from one another. In some embodiments, the promoter and second promoter are the same.

The nucleic acids described herein may further comprise a promoter operably linked to the sequence that encodes the Cas9 molecule of (b), e.g., a promoter described herein.

In another aspect, disclosed herein is a composition comprising (a) a gRNA molecule comprising a targeting domain that is complementary with a target domain in the UL19, UL30, UL48 or UL54 gene, as described herein. The composition of (a) may further comprise (b) a Cas9 molecule, e.g., a Cas9 molecule as described herein. A composition of (a) and (b) may further comprise (c) a second, third and/or fourth gRNA molecule, e.g., a second, third and/or fourth gRNA molecule described herein. In an embodiment, the composition is a pharmaceutical composition. The compositions described herein, e.g., pharmaceutical compositions described herein, can be used in the treatment or prevention of HSV-2 in a subject, e.g., in accordance with a method disclosed herein. In another aspect, disclosed herein is a method of altering a cell, e.g., altering the structure, e.g., altering the sequence, of a target nucleic acid of a cell, comprising contacting said cell with: (a) a gRNA that targets the UL19, UL30, UL48 or UL54 gene, e.g., a gRNA as described herein; (b) a Cas9 molecule, e.g., a Cas9 molecule as described herein; and optionally, (c) a second, third and/or fourth gRNA that targets UL19, UL30, UL48 or UL54 gene, e.g., a second, third and/or fourth gRNA, as described herein.

In an embodiment, the method comprises contacting said cell with (a) and (b).

In an embodiment, the method comprises contacting said cell with (a), (b), and (c).

The gRNA of (a) and optionally (c) may be selected from any of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A- 7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C, or a gRNA that differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from any of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A- 16C.

In an embodiment, the method comprises contacting a cell from a subject suffering from or likely to develop HSV-2. The cell may be from a subject that would benefit from having a mutation at a HSV-2 target position.

In an embodiment, the contacting step may be performed in vivo.

In an embodiment, the method of altering a cell as described herein comprises acquiring knowledge of the sequence of a HSV-2 target position in said cell, prior to the contacting step. Acquiring knowledge of the sequence of a HSV-2 target position in the cell may be by sequencing one or more of the UL19, UL30, UL48 or UL54 gene, or a portion of the UL19, UL30, UL48 and/or UL54 gene.

In an embodiment, the contacting step of the method comprises contacting the cell with a nucleic acid, e.g., a vector, e.g., an AAV vector, that expresses at least one of (a), (b), and (c). In some embodiments, the contacting step of the method comprises contacting the cell with a nucleic acid, e.g., a vector, e.g., an AAV vector, that expresses each of (a), (b), and (c). In another embodiment, the contacting step of the method comprises delivering to the cell a Cas9 molecule of (b) and a nucleic acid which encodes a gRNA of (a) and optionally, a second gRNA (c)(i), and further optionally, a third gRNA (c)(ii) and/or fourth gRNA (c)(iii). In an embodiment, the contacting step of the method comprises contacting the cell with a nucleic acid, e.g., a vector, e.g., an AAV vector, that expresses at least one of (a), (b), (c) and (d). In some embodiments, the contacting step of the method comprises contacting the cell with a nucleic acid, e.g., a vector, e.g., an AAV vector, that expresses each of (a), (b), and (c). In another embodiment, the contacting step of the method comprises delivering to the cell a Cas9 molecule of (b), a nucleic acid which encodes a gRNA of (a) and a template nucleic acid of (d), and optionally, a second gRNA (c)(i), and further optionally, a third gRNA (c)(iv) and/or fourth gRNA (c)(iii).

In an embodiment, contacting comprises contacting the cell with a nucleic acid, e.g., a vector, e.g., an AAV vector, e.g., an AAV2 vector, a modified AAV2 vector, an AAV3 vector, a modified AAV3 vector, an AAV6 vector, a modified AAV6 vector, an AAV8 vector or an AAV9 vector, as described herein.

In an embodiment, contacting comprises delivering to the cell a Cas9 molecule of (b), as a protein or an mRNA, and a nucleic acid which encodes a gRNA of (a) and optionally a second, third and/or fourth gRNA of (c).

In an embodiment, contacting comprises delivering to the cell a Cas9 molecule of (b), as a protein or an mRNA, said gRNA of (a), as an RNA, and optionally said second, third and/or fourth gRNA of (c), as an RNA.

In an embodiment, contacting comprises delivering to the cell a gRNA of (a) as an RNA, optionally the second, third and/or fourth gRNA of (c) as an RNA, and a nucleic acid that encodes the Cas9 molecule of (b).

In another aspect, disclosed herein is a method of treating a subject suffering from or likely to develop HSV-2, e.g., altering the structure, e.g., sequence, of a target nucleic acid of the subject, comprising contacting the subject (or a cell from the subject) with:

(a) a gRNA that targets the UL19, UL30, UL48 or UL54 gene, e.g., a gRNA disclosed herein;

(b) a Cas9 molecule, e.g., a Cas9 molecule disclosed herein; and

optionally, (c)(i) a second gRNA that targets the UL19, UL30, UL48 or UL54 gene, e.g., a second gRNA disclosed herein, and

further optionally, (c)(ii) a third gRNA, and still further optionally, (c)(iii) a fourth gRNA that target the UL19, UL30, UL48 or UL54 gene, e.g., a third and fourth gRNA disclosed herein.

In some embodiments, contacting comprises contacting with (a) and (b).

In some embodiments, contacting comprises contacting with (a), (b), and (c)(i). In some embodiments, contacting comprises contacting with (a), (b), (c)(i) and (c)(ii). In some embodiments, contacting comprises contacting with (a), (b), (c)(i), (c)(ii) and

(c)(iii).

The targeting domain of the gRNA of (a) or (c) (e.g., (c)(i), (c)(ii), or (c)(iii) may be selected from any of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C, or a targeting domain of a gRNA that differs by no more than 1, 2, 3, 4, or 5 nucleotides from, a targeting domain sequence from any of Tables 1A-1G, Tables 2A- 2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A- 13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C.

In an embodiment, the method comprises acquiring knowledge of the sequence at a HSV-2 target position in said subject.

In an embodiment, the method comprises acquiring knowledge of the sequence at a

HSV-2 target position in said subject by sequencing one or more of the UL19, UL30, UL48 and/or UL54 gene(s) or a portion of the UL19, UL30, UL48md/ or UL54 gene(s).

In an embodiment, the method comprises introducing a mutation at a HSV-2 target position.

In an embodiment, the method comprises introducing a mutation at a HSV-2 target position by NHEJ.

In an embodiment, a cell of the subject is contacted is in vivo with (a), (b) and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the cell of the subject is contacted in vivo by intravenous delivery of (a), (b), and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises contacting the subject with a nucleic acid, e.g., a vector, e.g., an AAV vector, described herein, e.g., a nucleic acid that encodes at least one of (a), (b), and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises delivering to said subject said Cas9 molecule of (b), as a protein or mRNA, and a nucleic acid which encodes (a), and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises delivering to the subject the Cas9 molecule of (b), as a protein or mRNA, the gRNA of (a), as an RNA, and optionally the second gRNA of (c)(i), further optionally (c)(ii), and still further optionally (c)(iii), as an RNA.

In an embodiment, the contacting step comprises delivering to the subject the gRNA of (a), as an RNA, optionally said second gRNA of (c)(i), further optionally (c)(ii), and still further optionally (c)(iii), as an RNA, a nucleic acid that encodes the Cas9 molecule of (b).

When the method comprises (1) introducing a mutation at a HSV-2 target position by NHEJ or (2) knocking down expression of one or more of the UL19, UL30, UL48 and/or UL54 gene(s), e.g., by targeting the promoter region, a Cas9 molecule of (b) and at least one guide RNA, e.g., a guide RNA of (a) are included in the contacting step.

In an embodiment, a cell of the subject is contacted is in vivo with (a), (b) and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii). In an embodiment, the cell of the subject is contacted in vivo by intravenous delivery of (a), (b) and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises contacting the subject with a nucleic acid, e.g., a vector, e.g., an AAV vector, described herein, e.g., a nucleic acid that encodes at least one of (a), (b), and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises delivering to said subject said Cas9 molecule of (b), as a protein or mRNA, and a nucleic acid which encodes (a) and optionally (c)(i), further optionally (c)(ii), and still further optionally (c)(iii).

In an embodiment, the contacting step comprises delivering to the subject the Cas9 molecule of (b), as a protein or mRNA, the gRNA of (a), as an RNA, and optionally the second gRNA of (c)(i), further optionally (c)(ii), and still further optionally (c)(iii), as an RNA.

In an embodiment, the contacting step comprises delivering to the subject the gRNA of (a), as an RNA, optionally said second gRNA of (c)(i), further optionally (c)(ii), and still further optionally (c)(iii), as an RNA, and a nucleic acid that encodes the Cas9 molecule of (b).

In another aspect, disclosed herein is a reaction mixture comprising a gRNA molecule, a nucleic acid, or a composition described herein, and a cell, e.g., a cell from a subject having, or likely to develop HSV-2, or a subject which would benefit from a mutation at a HSV-2 target position.

In another aspect, disclosed herein is a kit comprising, (a) a gRNA molecule described herein, or nucleic acid that encodes the gRNA, and one or more of the following: (b) a Cas9 molecule, e.g., a Cas9 molecule described herein, or a nucleic acid or mRNA that encodes the Cas9;

(c) (i) a second gRNA molecule, e.g., a second gRNA molecule described herein or a nucleic acid that encodes (c)(i);

(c)(ii) a third gRNA molecule, e.g., a second gRNA molecule described herein or a nucleic acid that encodes (c)(ii);

(c)(iii) a fourth gRNA molecule, e.g., a second gRNA molecule described herein or a nucleic acid that encodes (c)(iii).

In an embodiment, the kit comprises nucleic acid, e.g., an AAV vector, that encodes one or more of (a), (b), (c)(i), (c)(ii), and (c)(iii).

In yet another aspect, disclosed herein is a gRNA molecule, e.g., a gRNA molecule described herein, for use in treating, or delaying the onset or progression of HSV-2 infection in a subject, e.g., in accordance with a method of treating, or delaying the onset or progression of HSV-2 infection as described herein.

In an embodiment, the gRNA molecule in used in combination with a Cas9 molecule, e.g., a Cas9 molecule described herein. Additionally or alternatively, in an embodiment, the gRNA molecule is used in combination with a second, third and/or fouth gRNA molecule, e.g., a second, third and/or fouth gRNA molecule described herein.

In still another aspect, disclosed herein is use of a gRNA molecule, e.g., a gRNA molecule described herein, in the manufacture of a medicament for treating, or delaying the onset or progression of HSV-2 in a subject, e.g., in accordance with a method of treating, or delaying the onset or progression of HSV-2 as described herein.

In an embodiment, the medicament comprises a Cas9 molecule, e.g., a Cas9 molecule described herein. Additionaly or alternatively, in an embodiment, the medicament comprises a second, third and/or fouth gRNA molecule, e.g., a second, third and/or fouth gRNA molecule described herein.

The gRNA molecules and methods, as disclosed herein, can be used in combination with a governing gRNA molecule. As used herein, a governing gRNA molecule refers to a gRNA molecule comprising a targeting domain which is complementary to a target domain on a nucleic acid that encodes a component of the CRISPR/Cas system introduced into a cell or subject. For example, the methods described herein can further include contacting a cell or subject with a governing gRNA molecule or a nucleic acid encoding a governing molecule. In an embodiment, the governing gRNA molecule targets a nucleic acid that encodes a Cas9 molecule or a nucleic acid that encodes a target gene gRNA molecule. In an embodiment, the governing gRNA comprises a targeting domain that is complementary to a target domain in a sequence that encodes a Cas9 component, e.g., a Cas9 molecule or target gene gRNA molecule. In an embodiment, the target domain is designed with, or has, minimal homology to other nucleic acid sequences in the cell, e.g., to minimize off-target cleavage. For example, the targeting domain on the governing gRNA can be selected to reduce or minimize off-target effects. In an embodiment, a target domain for a governing gRNA can be disposed in the control or coding region of a Cas9 molecule or disposed between a control region and a transcribed region. In an embodiment, a target domain for a governing gRNA can be disposed in the control or coding region of a target gene gRNA molecule or disposed between a control region and a transcribed region for a target gene gRNA. While not wishing to be bound by theory, in an embodiment, it is believed that altering, e.g., inactivating, a nucleic acid that encodes a Cas9 molecule or a nucleic acid that encodes a target gene gRNA molecule can be effected by cleavage of the targeted nucleic acid sequence or by binding of a Cas9 molecule/governing gRNA molecule complex to the targeted nucleic acid sequence.

The compositions, reaction mixtures and kits, as disclosed herein, can also include a governing gRNA molecule, e.g., a governing gRNA molecule disclosed herein.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

Headings, including numeric and alphabetical headings and subheadings, are for organization and presentation and are not intended to be limiting.

Other features and advantages of the invention will be apparent from the detailed description, drawings, and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

Figs. 1A-1I are representations of several exemplary gRNAs.

Fig. 1A depicts a modular gRNA molecule derived in part (or modeled on a sequence in part) from Streptococcus pyogenes (S. pyogenes) as a duplexed structure (SEQ ID NOS: 42 and 43, respectively, in order of appearance); Fig. IB depicts a unimolecular (or chimeric) gRNA molecule derived in part from S. pyogenes as a duplexed structure (SEQ ID NO: 44);

Fig. 1C depicts a unimolecular gRNA molecule derived in part from S. pyogenes as a duplexed structure (SEQ ID NO: 45);

Fig. ID depicts a unimolecular gRNA molecule derived in part from S. pyogenes as a duplexed structure (SEQ ID NO: 46);

Fig. IE depicts a unimolecular gRNA molecule derived in part from S. pyogenes as a duplexed structure (SEQ ID NO: 47);

Fig. IF depicts a modular gRNA molecule derived in part from Streptococcus thermophilus (S. thermophilus) as a duplexed structure (SEQ ID NOS: 48 and 49, respectively, in order of appearance);

Fig. 1G depicts an alignment of modular gRNA molecules of S. pyogenes and S. thermophilus (SEQ ID NOS: 50-53, respectively, in order of appearance).

Figs. 1H-1I depicts additional exemplary structures of unimolecular gRNA molecules. Fig. 1H shows an exemplary structure of a unimolecular gRNA molecule derived in part from S. pyogenes as a duplexed structure (SEQ ID NO: 45). Fig. II shows an exemplary structure of a unimolecular gRNA molecule derived in part from S. aureus as a duplexed structure (SEQ ID NO: 40).

Figs. 2A-2G depict an alignment of Cas9 sequences from Chylinski et al. (RNA Biol. 2013; 10(5): 726-737). The N-terminal RuvC-like domain is boxed and indicated with a "Y". The other two RuvC-like domains are boxed and indicated with a "B". The HNH-like domain is boxed and indicated by a "G". Sm: S. mutans (SEQ ID NO: 1); Sp: S. pyogenes (SEQ ID NO: 2); St: 5". thermophilus (SEQ ID NO: 3); Li: L. innocua (SEQ ID NO: 4).

Motif: this is a motif based on the four sequences: residues conserved in all four sequences are indicated by single letter amino acid abbreviation; "*" indicates any amino acid found in the corresponding position of any of the four sequences; and "-" indicates any amino acid, e.g., any of the 20 naturally occurring amino acids, or absent.

Figs. 3A-3B show an alignment of the N-terminal RuvC-like domain from the Cas9 molecules disclosed in Chylinski et al (SEQ ID NOS: 54-103, respectively, in order of appearance). The last line of Fig. 3B identifies 4 highly conserved residues.

Figs. 4A-4B show an alignment of the N-terminal RuvC-like domain from the Cas9 molecules disclosed in Chylinski et al. with sequence outliers removed (SEQ ID NOS: 104- 177, respectively, in order of appearance). The last line of Fig. 4B identifies 3 highly conserved residues. Figs. 5A-5C show an alignment of the HNH-like domain from the Cas9 molecules disclosed in Chylinski et al (SEQ ID NOS: 178-252, respectively, in order of appearance). The last line of Fig. 5C identifies conserved residues.

Figs. 6A-6B show an alignment of the HNH-like domain from the Cas9 molecules disclosed in Chylinski et al. with sequence outliers removed (SEQ ID NOS: 253-302, respectively, in order of appearance). The last line of Fig. 6B identifies 3 highly conserved residues.

Figs. 7A-7B depict an alignment of Cas9 sequences from S. pyogenes and Neisseria meningitidis (TV. meningitidis). The N-terminal RuvC-like domain is boxed and indicated with a "Y". The other two RuvC-like domains are boxed and indicated with a "B". The

HNH-like domain is boxed and indicated with a "G". Sp: S. pyogenes; Nm: N. meningitidis. Motif: this is a motif based on the two sequences: residues conserved in both sequences are indicated by a single amino acid designation; "*" indicates any amino acid found in the corresponding position of any of the two sequences; "-" indicates any amino acid, e.g., any of the 20 naturally occurring amino acids, and "-" indicates any amino acid, e.g., any of the 20 naturally occurring amino acids, or absent.

Fig. 8 shows a nucleic acid sequence encoding Cas9 of N. meningitidis (SEQ ID NO: 303). Sequence indicated by an "R" is an SV40 NLS; sequence indicated as "G" is an HA tag; and sequence indicated by an "O" is a synthetic NLS sequence; the remaining

(unmarked) sequence is the open reading frame (ORF).

Figs. 9A and 9B are schematic representations of the domain organization of S.

pyogenes Cas 9. Fig. 9A shows the organization of the Cas9 domains, including amino acid positions, in reference to the two lobes of Cas9 (recognition (REC) and nuclease (NUC) lobes). Fig. 9B shows the percent homology of each domain across 83 Cas9 orthologs.

Definitions

"Domain", as used herein, is used to describe segments of a protein or nucleic acid. Unless otherwise indicated, a domain is not required to have any specific functional property.

Calculations of homology or sequence identity between two sequences (the terms are used interchangeably herein) are performed as follows. The sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The optimal alignment is determined as the best score using the GAP program in the GCG software package with a Blossum 62 scoring matrix with a gap penalty of 12, a gap extend penalty of 4, and a frame shift gap penalty of 5. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences.

"Governing gRNA molecule", as used herein, refers to a gRNA molecule that comprises a targeting domain that is complementary to a target domain on a nucleic acid that comprises a sequence that encodes a component of the CRISPR/Cas system that is introduced into a cell or subject. A governing gRNA does not target an endogenous cell or subject sequence. In an embodiment, a governing gRNA molecule comprises a targeting domain that is complementary with a target sequence on: (a) a nucleic acid that encodes a Cas9 molecule; (b) a nucleic acid that encodes a gRNA which comprises a targeting domain that targets the UL19, UL30, UL48 or UL54 gene (a target gene gRNA); or on more than one nucleic acid that encodes a CRISPR/Cas component, e.g., both (a) and (b). In an embodiment, a nucleic acid molecule that encodes a CRISPR/Cas component, e.g., that encodes a Cas9 molecule or a target gene gRNA, comprises more than one target domain that is complementary with a governing gRNA targeting domain. While not wishing to be bound by theory, in an embodiment, it is believed that a governing gRNA molecule complexes with a Cas9 molecule and results in Cas9 mediated inactivation of the targeted nucleic acid, e.g., by cleavage or by binding to the nucleic acid, and results in cessation or reduction of the production of a CRISPR/Cas system component. In an embodiment, the Cas9 molecule forms two complexes: a complex comprising a Cas9 molecule with a target gene gRNA, which complex will alter the UL19, UL30, UL48 or UL54 gene; and a complex comprising a Cas9 molecule with a governing gRNA molecule, which complex will act to prevent further production of a CRISPR/Cas system component, e.g., a Cas9 molecule or a target gene gRNA molecule. In an embodiment, a governing gRNA molecule/Cas9 molecule complex binds to or promotes cleavage of a control region sequence, e.g., a promoter, operably linked to a sequence that encodes a Cas9 molecule, a sequence that encodes a transcribed region, an exon, or an intron, for the Cas9 molecule. In an embodiment, a governing gRNA molecule/Cas9 molecule complex binds to or promotes cleavage of a control region sequence, e.g., a promoter, operably linked to a gRNA molecule, or a sequence that encodes the gRNA molecule. In an embodiment, the governing gRNA, e.g., a Cas9-targeting governing gRNA molecule, or a target gene gRNA-targeting governing gRNA molecule, limits the effect of the Cas9 molecule/target gene gRNA molecule complex-mediated gene targeting. In an embodiment, a governing gRNA places temporal, level of expression, or other limits, on activity of the Cas9 molecule/target gene gRNA molecule complex. In an embodiment, a governing gRNA reduces off-target or other unwanted activity. In an embodiment, a governing gRNA molecule inhibits, e.g., entirely or substantially entirely inhibits, the production of a component of the Cas9 system and thereby limits, or governs, its activity.

"Modulator", as used herein, refers to an entity, e.g., a drug, that can alter the activity (e.g., enzymatic activity, transcriptional activity, or translational activity), amount, distribution, or structure of a subject molecule or genetic sequence. In an embodiment, modulation comprises cleavage, e.g., breaking of a covalent or non-covalent bond, or the forming of a covalent or non-covalent bond, e.g., the attachment of a moiety, to the subject molecule. In an embodiment, a modulator alters the, three dimensional, secondary, tertiary, or quaternary structure, of a subject molecule. A modulator can increase, decrease, initiate, or eliminate a subject activity.

"Large molecule", as used herein, refers to a molecule having a molecular weight of at least 2, 3, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 kD. Large molecules include proteins, polypeptides, nucleic acids, biologies, and carbohydrates.

"Polypeptide", as used herein, refers to a polymer of amino acids having less than 100 amino acid residues. In an embodiment, it has less than 50, 20, or 10 amino acid residues.

"Reference molecule", e.g., a reference Cas9 molecule or reference gRNA, as used herein, refers to a molecule to which a subject molecule, e.g., a subject Cas9 molecule of subject gRNA molecule, e.g., a modified or candidate Cas9 molecule is compared. For example, a Cas9 molecule can be characterized as having no more than 10% of the nuclease activity of a reference Cas9 molecule. Examples of reference Cas9 molecules include naturally occurring unmodified Cas9 molecules, e.g., a naturally occurring Cas9 molecule such as a Cas9 molecule of S. pyogenes, S. aureus or S. thermophilus. In an embodiment, the reference Cas9 molecule is the naturally occurring Cas9 molecule having the closest sequence identity or homology with the Cas9 molecule to which it is being compared. In an embodiment, the reference Cas9 molecule is a sequence, e.g., a naturally occurring or known sequence, which is the parental form on which a change, e.g., a mutation has been made.

"Replacement", or "replaced", as used herein with reference to a modification of a molecule does not require a process limitation but merely indicates that the replacement entity is present. "Small molecule", as used herein, refers to a compound having a molecular weight less than about 2 kD, e.g., less than about 2 kD, less than about 1.5 kD, less than about 1 kD, or less than about 0.75 kD.

"Subject", as used herein, may mean either a human or non-human animal. The term includes, but is not limited to, mammals (e.g., humans, other primates, pigs, rodents (e.g., mice and rats or hamsters), rabbits, guinea pigs, cows, horses, cats, dogs, sheep, and goats). In an embodiment, the subject is a human. In other embodiments, the subject is poultry.

"Treat", "treating" and "treatment", as used herein, mean the treatment of a disease in a mammal, e.g., in a human, including (a) inhibiting the disease, i.e., arresting or preventing its development; (b) relieving the disease, i.e., causing regression of the disease state; and (c) curing the disease.

"Prevent", "preventing" and "prevention", as used herein, means the prevention of a disease in a mammal, e.g., in a human, including (a) avoiding or precluding the disease; (2) affecting the predisposition toward the disease, e.g., preventing at least one symptom of the disease or to delay onset of at least one symptom of the disease.

"X" as used herein in the context of an amino acid sequence, refers to any amino acid (e.g., any of the twenty natural amino acids) unless otherwise specified.

Herpes simplex virus type 2

Herpes simplex virus type 2 (HSV-2) is a sexually transmitted virus. It is most commonly known as genital herpes. Initial infection with HSV-2 generally causes painful blistering in the genital region. The disease causes lifelong, recurring bouts of viral reactivity. It is highly contagious and increases the risk of acquiring HIV infection, especially among patients with active lesions. More than 500 million people are infected with HSV-2 worldwide. Up to 23 million individuals are infected for the first time each year. In the United States, approximately 16% of the population aged 14-49 is seropositive for HSV-2 infection.

HSV-2 infection persists for the lifetime of the host. Primary and re-activation infections can cause permanent neurologic sequelae and blindness. HSV-2 also increases a subject's risk of developing HIV. There is a considerable need for methods to treat and prevent HSV-2 infections.

During primary infection, the virus most often infects cells of the ano-genital region, causing painful vesicles in the affected region. Re-activation of HSV-2 infections most often occurs in the ano-genital region. Re-activation infections of the eye and central nervous system are the most severe and damaging HSV manifestations, as they can lead to blindness and permanent neurologic disability, respectively.

HSV-2 is contained within an icosahedral particle. The virus enters the host via infection of epithelial cells within the skin and mucous membranes. The virus produces immediate early genes within the epithelial cells, which encode enzymes and binding proteins necessary for viral synthesis. After primary infection, the virus travels up sensory nerve axons via retrograde transport to the sensory dorsal root ganglion (DRG), most commonly a sacral DRG. HSV-2 mainly travels to the sensory DRG located within the sacrum, but can travel to other sensory ganglia depending upon the site of primary infection. Within the DRG, it establishes a latent infection. The latent infection persists for the lifetime of the host. This means that the virus uncoats, viral DNA is transported into the nucleus of the sensory nerve, and key viral RNAs associated with latency are transcribed (i.e., the LAT RNAs).

Host immune defense is very important to combating HSV infection. CD4+ T-cells and CD8+ cells are responsible for recognizing and clearing the pathogen. Subjects with impaired T-cell responses- including those with HIV, those receiving immunosuppressants following organ transplants, and neonates with developing immune systems are subject to the most severe manifestations of HSV-2 infections.

During a primary infection, subjects experience painful blistering in the genital region that may last 4-15 days. The blisters may break, releasing clear fluid that is highly infectious. The sores most commonly involve the vagina, labia, cervix, penis, scrotum, anus and skin around the thighs. HSV-2 primary infections may also involve the oral region, including the lips, gums and nasal mucous membranes. Less commonly, HSV-2 primary infection may involve the eyes, central nervous system, fingers and fingernail beds (herpetic whitlow). HSV-2 infection is transmitted primarily through sexual activity. The blisters may break, releasing clear fluid that is highly infectious. Primary infection is often accompanied by a flu-like illness, including fever, chills and muscle aches.

Reactivations of latent infections are generally less severe and may be of shorter duration. Reactivation can affect the oral region, the ano-genital region, the eye, the central nervous system, the fingernails, and the pharynx. Reactivation generally affects the genital region but can also cause ophthalmologic disease, including keratitis (epithelial keratitis, stromal keratitis and disciform keratitis). Generally, ophthalmologic manifestations of HSV-2 are self-limiting. However, HSV-2 keratitis may rarely cause scarring, secondary infection with bacterial pathogens and rarely, blindness. In some cases, reactivation can occur in the central nervous system (CNS) via retrograde transport of the virus into the CNS. Generally, patients who are immune compromised develop HSV-2 induced encephalitis or meningitis. HSV-2 encephalitis and meningitis are both extremely severe. Subjects generally experience permanent neurologic damage in spite of treatment with antiviral therapy.

Primary infections and reactivation infections in the central nervous system (CNS), called encephalitis and meningitis, are particularly damaging. In a study of infants with HSV-encephalitis or meningitis treated with high dose antiviral therapy, there was found to be a 4% mortality rate and 69% of survivors had permanent neurologic sequelae (Kimberlin et al., Pediatrics. 2001; 108: 230-238). The majority of infants and adults who develop HSV- 2 encephalitis or meningitis will experience permanent neurologic damage in spite of treatment with antiviral therapy. Reactivation infections in the CNS occur via anterograde transport of the virus into the CNS. Most commonly, patients who are immune compromised or infants develop HSV-2 induced encephalitis and/or meningitis. Healthy adults more rarely develop HSV-2 disease of the CNS.

In subjects with HSV-2 infections, the virus generally establishes latency in the sacral ganglia, leading to re-activation infection of the genital region.

Re-activation may also occur in the eye via anterograde transport of the virus from the trigeminal ganglion, along the ophthalmic branch of the trigeminal nerve (the fifth cranial nerve) and into the eye. Re-activation of the virus may also occur from within the cornea. Latency within the trigeminal ganglion is established via one of two mechanisms. First, HSV-2 can travel via retrograde transport along the trigeminal nerve from the eye (after an eye infection) into the trigeminal ganglion. Alternatively, it can spread to the trigeminal ganglion via hematogenous spread following infection of the oral mucosa, genital region, or other extraocular site. After establishing latent infection of the trigeminal ganglion, at any time, particularly in the event of an immunocompromised host, the virus can re-establish infection by traveling anterograde along the trigeminal nerve and into the eye.

Ocular herpes can affect the anterior chamber of the eye, where it causes keratitis, or the posterior chamber, where it causes retinitis. In neonates and children, HSV-2 is responsible for the majority of cases of HSV-retinitis (Pepose et al., Ocular Infection and Immunity 1996; Mosby 1155-1168). HSV-2 retinitis can lead to acute retinal necrosis (ARN), which will destroy the retina within 2 weeks without treatment (Banerjee and Rouse, Human Herpesviruses 2007; Cambridge University Press, Chapter 35). Even with treatment, the risk of permanent visual damage following ARN is higher than 50% (Roy et al., Ocular Immunology and Inflammation 2014; 22(3): 170-174).

Keratitis is the most common form of ocular herpes. HSV-2 keratitis can manifest as dentritic keratitis, stromal keratitis, blepharatis and conjunctivitis. In the United States, there are approximately 48,000 cases of recurrent or primary HSV-related keratitis infections annually (Liesegang et. al., 1989; 107(8): 1155-1 159). Of all cases of HSV-related keratitis, approximately 1.5-3% of subjects experience severe, permanent visual impairment

(Wiihelmus et. al., Archives of Ophthalmology 1981; 99(9): 1578-82).

Overall, stromal keratitis represents approximately 15% of keratitis cases and is associated with the highest risk of permanent visual damage. Stromal keratitis results in scarring and irregular astimagtism. Previous ocular HSV infection increases the risk for developing stromal infection, which means that subjects who have had a prior ocular HSV infection have an increased risk for permanent visual damage on reactivation. In children, stromal keratitis represents up to 60% of all keratitis cases so children are particularly at risk for permanent visual damage from HSV-associated keratitis. A retrospective study in the United States from 1950-1982 found that there are approximately 2.6 new or recurrent stromal keratitis cases per 100,000 person years, or approximately 8,000 cases of stromal keratitis annually (Liesegang et. al., 1989: 107(8): 1 155-1159). A more recent study in France in 2002 estimated the incidence of new or recurrent stromal keratitis cases to be 9.6 per 100,000 (Labetoulle et al., Ophthalmology 2005; 11.2(5):888-895). The incidence of

HSV-associated keratitis may be increasing in the developed world (Farooq and Shukla 2012; Survey of Ophthalmology 57(5): 448-462).

The compositions and methods described herein can be used for the treatment and prevention of HSV-2 infections, including but not limited to HSV-2 stromal keratitis, HSV-2 retinitis, HSV-2 encephalitis and HSV-2 meningitis.

Newborns are a population at particular risk for developing severe HSV-2 infections. The disease is transmitted from the mother to the fetus during childbirth. The chance of maternal-fetal transmission is highest in cases where the mother developed primary HSV infection during pregnancy. The incidence of neonatal herpes is approximately 4-30 per 100,000 births. Neonates may develop severe HSV-2 encephalitis and/or meningitis. In spite of prompt treatment with antiviral therapy, the rate of permanent neurologic sequelae in newborns infected with HSV-2 is significant.

HSV-2 infection is treated with antiviral therapy, including acyclovir, valacyclovir and famciclovir. These therapies have been demonstrated to reduce viral shedding, decrease pain and improve healing time of lesions. Therapy is primarily given during acute infection and there is no curative treatment. Therapy may be given prophylactically in certain situations, including during childbirth in a mother with a recent HSV-2 infection or reactivation.

There is no effective therapy that prevents HSV-2 infection. The use of antiviral therapy during active infection and the use of condoms decrease transmission rates by approximately 50%.

Human immunodeficiency virus-1 (HIV-1) acquisition rates are dramatically increased in subjects who are seropositive for HSV-2. The risk of infection with HIV-1 is 3- fold higher in subjects with HSV-2. Antivirals have no impact on reducing risk of HIV acquisition.

Methods to Treat, Prevent or Reduce HSV-2 Infection

Disclosed herein are the approaches to treat, prevent, or reduce HSV-2 infection, using the compositions and methods described herein.

HSV-2 relies on the genes UL19, UL30, UL48 and UL54 for infection, proliferation and assembly. Knockout of any of these genes singly or in combination can prevent and/or cure HSV-2 infections. As the HSV-2 virus establishes latency in discrete, localized regions within the body, it is highly amenable to local delivery that delivers a disabling treatment in the region of latency. Targeting knock-out to a discrete region or regions, (e.g., the trigeminal dorsal root ganglion, e.g., the cervical dorsal root ganglia, e.g., the sacral dorsal root ganglia) can eliminate latent infection by disabling the HSV-2 virus.

Described herein are the approaches to treat or prevent HSV-2 by knocking out viral genes. Methods described herein include the knockout of the following HSV-2 encoded genes: UL19, UL30, UL48 and UL54, or any combination thereof (e.g., any two, three or all of the UL19, UL30, UL48 or UL54 gene).

UL19 (also known as VP5) encodes the HSV-2 major capsid protein, VP5. Proper assembly of the viral capsid is known to be an essential part of viral replication, assembly, maturation and infection (Homa et al.. Reviews of Medical Virology 1997; 7(2):107-122). RNAi-mediated knockdown of VP5 along with another capsid capsid protein, VP23, in vitro, greatly diminished HSV proliferation (Jin et al., PLoS One 2014; 9(5): e96623). Knockout of UL19 can disable HSV-2 proliferation and therefore prevent and/or cure HSV-2 infection. UL30 encodes the DNA polymerase catalytic subunit (HSV-2 pol). The 5' domain of HSV-2 pol is required for viral replication. Knockout of UL30 can disable HSV-2 replication and therefore prevent and/or cure HSV-2 infection.

UL48 encodes the viral protein known as VP16 in HSV-2. VP-16 has been shown to be important in viral egress, the process by which the assembled viral capsid leaves the host nucleus and enters the cytoplasm (Mossman et al., Journal of Virology 2000; 74(14): 6287- 6289). Mutation of UL48 in cell culture decreased the ability of HSV to assemble efficiently (Svobodova et al., Journal of Virology 2012; 86(1): 473-483). Knockout of UL48 can disable HSV-2 assembly and egress and therefore prevent and/or cure HSV-2 infection.

UL54 encodes ICP27, a highly conserved, multi-functional protein. ICP27 is involved in transcription, RNA processing, RNA export and translation (Sandri-Goldin, Frontiers in Bioscience 2008; 13:5241-5256). ICP27 also shuts off host gene expression during HSV-2 infection. Knock out of UL54 can disable HSV-2 transcription, translation and RNA processing and therefore prevent and/or cure HSV-2 infection.

Knockout of the genes UL19, UL30, UL48 and UL54, individually or in combination, can reduce HSV-2 infectivity, replication, or packaging and can therefore prevent or treat HSV-2 infection.

In addition, knock out of vital HSV-2 genes, e.g., UL19, UL30, UL48 and UL54, individually or in combination can make HSV-2 more susceptible to antiviral therapy.

Mutations in important genes can render HSV-2 and other viruses more susceptible to treatment with antivirals (Zhou et al., Journal of Virology 2014; 88(19): 11121-11129).

Knocking-out UL19, UL30, UL48 and UL54, individually or in combination may be combined with an antiviral therapy to prevent or treat cure HSV-2 infection. The

compositions and methods described herein can be used in combination with another antiviral therapy, e.g., another anti-HSV-2 therapy described herein, to treat or prevent HSV-2 infection.

In one approach, one or more of the UL19, UL30, UL48 and/or UL54 gene(s) is targeted as a targeted knockout, e.g., to inhibit essential viral functions, including, e.g. viral gene transcription, viral genome replication and viral capsid formation. In an embodiment, said approach comprises knocking out one HSV-2 gene (e.g., UL19, UL30, UL48 or UL54 gene). In another embodiment, said approach comprises knocking out two HSV-2 genes, e.g., two of UL19, UL30, UL48 or UL54 gene(s). In another embodiment, said approach comprises knocking out three HSV-2 genes, e.g., three or more of UL19, UL30, UL48 or UL54 gene(s). In another embodiment, said approach comprises knocking out four HSV-2 genes, e.g., each of UL19, UL30, UL48 and UL54 gene(s).

While not wishing to be bound by theory, it is considered that inhibiting essential viral functions, e.g., viral gene transcription, viral genome replication and viral capsid formation, decreases the duration of primary or recurrent infection and/or decrease shedding of viral particles. Subjects also experience shorter duration(s) of illness, decreased risk of transmission to sexual partners, decreased risk of transmission to the fetus in the case of pregnancy and/or the potential for full clearance of HSV-2 (cure).

Knockout of one or more copies (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more copies) of one or more target gene (e.g., UL19, UL30, UL48 or UL54 gene) may be performed prior to disease onset or after disease onset, but preferably early in the disease course.

In an embodiment, the method comprises initiating treatment of a subject prior to disease onset.

In an embodiment, the method comprises initiating treatment of a subject after disease onset.

In an embodiment, the method comprises initiating treatment of a subject well after disease onset, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 16, 24, 36, 48 or more months after onset of HSV-2 infection. In an embodiment, the method comprises initiating treatment of a subject well after disease onset, e.g., 1, 2, 3, 4, 5, 10, 15, 20, 25, 40, 50 or 60 years after onset of HSV-2 infection. While not wishing to be bound by theory it is believed that this may be effective if subjects present well into the course of illness.

In an embodiment, the method comprises initiating treatment of a subject in an advanced stage of disease, e.g., during acute or latent periods. In an embodiment, the method comprises initiating treatment of a subject in severe, acute stages of the disease affecting the central nervous system, eyes, oropharynx, genital region, and/or other regions.

Overall, initiation of treatment for subjects at all stages of disease is expected to improve healing, decrease duration of disease and be of benefit to subjects.

In an embodiment, the method comprises initiating treatment of a subject prior to disease expression. In an embodiment, the method comprises initiating treatment of a subject in an early stage of disease, e.g., when a subject has been exposed to HSV-2 or is thought to have been exposed to HSV-2.

In an embodiment, the method comprises initiating treatment of a subject prior to disease expression. In an embodiment, the method comprises initiating treatment of a subject in an early stage of disease, e.g., when a subject has tested positive for HSV-2 infections but has no signs or symptoms.

In an embodiment, the method comprises initiating treatment of a subject at the appearance of painful blistering in or around the mouth, e.g., oral or oropharynx, e.g., in an infant, child, adult or young adult.

In an embodiment, the method comprises initiating treatment of a subject at the appearance of painful blistering in the genital region, e.g., in an infant, child, adult or young adult.

In an embodiment, the method comprises initiating treatment of a subject suspected of having HSV-2 meningitis and/or HSV-2 encephalitis.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following symptoms consistent or associated with HSV-2 meningitis and/or encephalitis: fever, headache, vomiting, photophobia, seizure, decline in level of

consciousness, lethargy, or drowsiness.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following signs consistent or associated with HSV meningitis and/or encephalitis: positive CSF culture for HSV-2, elevated WBC in CSF, neck stiffness/positive Brudzinski' s sign. In an embodiment, the method comprises initiating treatment in a patient with signs consistent with HSV-2 encephalitis and/or meningitis on EEG, CSF exam, MRI, PCR of CSF specimen, and/or PCR of brain biopsy specimen.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following symptoms consistent or associated with optic HSV-2: pain, photophobia, blurred vision, tearing, redness/injection, loss of vision, floaters, or flashes.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following findings on ophthalmologic exam consistent or associated with optic HSV-2, also known as HSV-2 keratitis: small, raised clear vesicles on corneal epithelium; irregular corneal surface, punctate epithelial erosions; dense stromal infiltrate; ulceration; necrosis; focal, multifocal, or diffuse cellular infiltrates; immune rings; neovascularization; or ghost vessels at any level of the cornea.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following findings on ophthalmologic exam consistent or associated with HSV-2 retinitis or acute retinal necrosis: reduced visual acuity; uveitis; vitritis; scleral injection; inflammation of the anterior and/or vitreous chamber/s; vitreous haze; optic nerve edema; peripheral retinal whitening; retinal tear; retinal detachment; retinal necrosis; evidence of occlusive vasculopathy with arterial involvement, including arterioloar sheathing and arteriolar attenuation.

In an embodiment, the method comprises initiating treatment at the appearance of symptoms and/or signs consistent or associated with either an HSV-1 or an HSV-2 infection of the eye, oropharynx, ano-genital region or central nervous system. While not wishing to be bound by theory, intiating treatment for HSV-2 infection in a case of suspected HS V- 1 or HSV-2 infection early in the disease course is beneficial.

In an embodiment, the method comprises initiating treatment in utero in case of high risk of maternal-to-fetal transmission.

In an embodiment, the method comprises initiating treatment during pregnancy in case of mother who has active HSV-2 infection or has recent primary HSV-2 infection.

In an embodiment, the method comprises initiating treatment prior to organ transplantation or immediately following organ transplantation.

In an embodiment, the method comprises initiating treatment in case of suspected exposure to HSV-2.

In an embodiment, the method comprises initiating treatment prophylactically, especially in case of suspected HSV-encephalitis or meningitis.

In an embodiment, the method comprises initiating treatment of a subject who suffers from or is at risk of developing severe manifestations of HSV-2 infections, e.g., neonates, subjects with HIV, subjects who are on immunosuppressant therapy following organ transplantation, subjects who have cancer, subjects who are undergoing chemotherapy, subjects who will undergo chemotherapy, subjects who are undergoing radiation therapy, subjects who will undergo radiation therapy.

While not wishing to be bound by theory, it is considered that both HIV positive subjects and post-transplant subjects may experience severe HSV-2 activation or reactivation, including HSV-encephalitis and meningitis, due to immunodeficiency. Neonates are also at risk for severe HSV-encephalitis due to maternal-fetal transmission during childbirth.

Inhibiting essential viral functions, e.g., viral gene transcription, viral genome replication and viral capsid formation, may provide superior protection to said populations at risk for severe HSV-2 infections. Subjects may experience lower rates of HSV-2 encephalitis and/or lower rates of severe neurologic sequelae following HSV-2 encephalitis, which will profoundly improve quality of life.

In an embodiment, the method comprises initiating treatment of a subject who has tested positive for HSV-2. In an embodiment, the method comprises initiating treatment at the appearance of any one or more of the following findings consistent or associated with HSV-2: appearance of blistering in the ano-genital area, genital ulcers and/or flu-like illness.

In an embodiment, the method comprises initiating treatment at the appearance of any of the following findings consistent or associated with HSV-2 infection: fever, headache, body aches, ano-genital blistering, oral ulceration, encephalitis, or keratitis.

In an embodiment, the method comprises initiating treatment in a subject who has tested positive for HSV-2 infection via viral culture, direct fluorescent antibody study, skin biopsy, PCR, blood serologic test, CSF serologic test, CSF PCR, or brain biopsy. In an embodiment, the method comprises initiating treatment in a subject who has tested positive for HSV-2 infection via diagnostic vitrectomy, endoretinal biopsy, PCR of aqueous fluid, PCR of vitreous sample.

In an embodiment, the method comprises initiating treatment in any patient exposed to HSV-2 and at high risk for severe sequelae from HSV infection.

In some embodiments, a cell is manipulated by editing (e.g., introducing a mutation in) one or more target genes, e.g., UL19, UL30, UL48 or UL54 gene. In some embodiments, the expression of one or more target genes (e.g., one or more UL19, UL30, UL48 or UL54 gene described herein) is modulated, e.g., in vivo.

In an embodiment, the method comprises delivery of gRNA by an AAV. In an embodiment, the method comprises delivery of gRNA by a lentivirus. In an embodiment, the method comprises delivery of gRNA by a nanoparticle. In an embodiment, the method comprises delivery of gRNA by a gel-based AAV for topical therapy.

In an embodiment, the method further comprising treating the subject a second antiviral therapy, e.g., an anti-HSV-2 therapy described herein. The compositions described herein can be administered concurrently with, prior to, or subsequent to, one or more additional therapies or therapeutic agents. The composition and the other therapy or therapeutic agent can be administered in any order. In an embodiment, the effect of the two treatments is synergistic. Exemplary anti-HSV-2 therapies include, but are not limited to, acyclovir, valacyclovir, famciclovir, penciclovir, or a vaccine.

Methods of Altering UL19, UL30, UL48 and/or UL54 gene(s)

As disclosed herein, a HSV-2 target position, e.g., one or more of UL19, UL30, UL48 or UL54 gene(s), can be altered by gene editing, e.g., using CRISPR-Cas9 mediated methods as described herein. Methods and compositions discussed herein, provide for altering a HSV-2 target position in one or more of the UL19, UL30, UL48 and/or UL54 gene(s). A HSV-2 target position can be altered by gene editing, e.g., using CRISPR-Cas9 mediated methods to alter one or more of the UL19, UL30, UL48 and/or UL54 gene(s).

An alteration of one or more of the UL19, UL30, UL48 and/or UL54 gene(s) can be mediated by any mechanism. Exemplary mechanisms that can be associated with an alteration of one or more of the UL19, UL30, UL48 and/or UL54 gene(s) include, but are not limited to, non-homologous end joining (e.g., classical or alternative), microhomology- mediated end joining (MMEJ), homology-directed repair (e.g., endogenous donor template mediated), SDSA (synthesis dependent strand annealing), single strand annealing or single strand invasion.

In an embodiment, a single strand break is introduced (e.g., positioned by one gRNA molecule) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, a single gRNA molecule (e.g., with a Cas9 nickase) is used to create a single strand break at or in close proximity to the HSV-2 target position, e.g., the gRNA is configured such that the single strand break is positioned either upstream (e.g., within 200 bp upstream) or downstream (e.g., within 200 bp downstream) of the HSV-2 target position. In an embodiment, the break is positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, a double strand break is introduced (e.g., positioned by one gRNA molecule) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, a single gRNA molecule (e.g., with a Cas9 nuclease other than a Cas9 nickase) is used to create a double strand break at or in close proximity to the HSV-2 target position, e.g., the gRNA molecule is configured such that the double strand break is positioned either upstream (e.g., within 200 bp upstream) or downstream of (e.g., within 200 bp downstream) of a HSV-2 target position. In an embodiment, the break is positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, two single strand breaks are introduced (e.g., positioned by two gRNA molecules) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, two gRNA molecules (e.g., with one or two Cas9 nickcases) are used to create two single strand breaks at or in close proximity to the HSV-2 target position, e.g., the gRNAs molecules are configured such that both of the single strand breaks are positioned upstream (e.g., within 200 bp upstream) or downstream (e.g., within 200 bp downstream) of the HSV-2 target position. In another embodiment, two gRNA molecules (e.g., with two Cas9 nickcases) are used to create two single strand breaks at or in close proximity to the HSV-2 target position, e.g., the gRNAs molecules are configured such that one single strand break is positioned upstream (e.g., within 200 bp upstream) and a second single strand break is positioned downstream (e.g., within 200 bp downstream) of the HSV-2 target position. In an embodiment, the breaks are positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, two double strand breaks are introduced (e.g., positioned by two gRNA molecules) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, two gRNA molecules (e.g., with one or two Cas9 nucleases that are not Cas9 nickases) are used to create two double strand breaks to flank a HSV-2 target position, e.g., the gRNA molecules are configured such that one double strand break is positioned upstream (e.g., within 200 bp upstream) and a second double strand break is positioned downstream (e.g., within 200 bp downstream) of the HSV-2 target position. In an embodiment, the breaks are positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, one double strand break and two single strand breaks are introduced (e.g., positioned by three gRNA molecules) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, three gRNA molecules (e.g., with a Cas9 nuclease other than a Cas9 nickase and one or two Cas9 nickases) to create one double strand break and two single strand breaks to flank a HSV-2 target position, e.g., the gRNA molecules are configured such that the double strand break is positioned upstream or downstream of (e.g., within 200 bp upstream or downstream) of the HSV-2 target position, and the two single strand breaks are positioned at the opposite site, e.g., downstream or upstream (within 200 bp downstream or upstream), of the HSV-2 target position. In an embodiment, the breaks are positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, four single strand breaks are introduced (e.g., positioned by four gRNA molecules) at or in close proximity to a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene. In an embodiment, four gRNA molecule (e.g., with one or more

Cas9 nickases are used to create four single strand breaks to flank a HSV-2 target position in the UL19, UL30, UL48 and/or UL54 gene, e.g., the gRNA molecules are configured such that a first and second single strand breaks are positioned upstream (e.g., within 200 bp upstream) of the HSV-2 target position, and a third and a fourth single stranded breaks are positioned downstream (e.g., within 200 bp downstream) of the HSV-2 target position. In an embodiment, the breaks are positioned to avoid unwanted target chromosome elements, such as repeat elements, e.g., an Alu repeat.

In an embodiment, two or more (e.g., three or four) gRNA molecules are used with one Cas9 molecule. In another embodiment, when two or more (e.g., three or four) gRNAs are used with two or more Cas9 molecules, at least one Cas9 molecule is from a different species than the other Cas9 molecule(s). For example, when two gRNA molecules are used with two Cas9 molecules, one Cas9 molecule can be from one species and the other Cas9 molecule can be from a different species. Both Cas9 species are used to generate a single or double-strand break, as desired.

I. gRNA Molecules

A gRNA molecule, as that term is used herein, refers to a nucleic acid that promotes the specific targeting or homing of a gRNA molecule/Cas9 molecule complex to a target nucleic acid. gRNA molecules can be unimolecular (having a single RNA molecule), sometimes referred to herein as "chimeric" gRNAs, or modular (comprising more than one, and typically two, separate RNA molecules). A gRNA molecule comprises a number of domains. The gRNA molecule domains are described in more detail below.

Several exemplary gRNA structures, with domains indicated thereon, are provided in Fig. 1. While not wishing to be bound by theory, in an embodiment, with regard to the three dimensional form, or intra- or inter-strand interactions of an active form of a gRNA, regions of high complementarity are sometimes shown as duplexes in Figs. 1A-1G and other depictions provided herein.

In an embodiment, a unimolecular, or chimeric, gRNA comprises, preferably from 5' to 3':

a targeting domain (which is complementary to a target nucleic acid in the UL19, UL30, UL48 or UL54 gene, e.g., a targeting domain from any of Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E,

Tables 15A-15G, or Tables 16A-16C;

a first complementarity domain;

a linking domain;

a second complementarity domain (which is complementary to the first complementarity domain);

a proximal domain; and

optionally, a tail domain.

embodiment, a modular gRNA comprises:

a first strand comprising, preferably from 5' to 3'

a targeting domain (which is complementary to a target nucleic acid in the UL19, UL30, UL48 or UL54 gene, e.g., a targeting domain from Tables 1A-1G, Tables 2A-2F, Tables 3A-3F, Tables 4A-4G, Tables 5A-5E, Tables 6A-6G, Tables 7A-7D, Tables 8A-8E, Tables 9A-9G, Tables 10A-10D, Tables 11A-11E, Tables 12A-12G, Tables 13A-13B, Tables 14A-14E, Tables 15A-15G, or Tables 16A-16C); and

a first complementarity domain; and

a second strand, comprising, preferably from 5 ' to 3 ' :

optionally, a 5' extension domain;

a second complementarity domain;

a proximal domain; and

optionally, a tail domain.

The domains are discussed briefly below.

The Targeting Domain

Figs. 1A-1G provide examples of the placement of targeting domains.

The targeting domain comprises a nucleotide sequence that is complementary, e.g., at least 80, 85, 90, or 95% complementary, e.g., fully complementary, to the target sequence on the target nucleic acid. The targeting domain is part of an RNA molecule and will therefore comprise the base uracil (U), while any DNA encoding the gRNA molecule will comprise the base thymine (T). While not wishing to be bound by theory, in an embodiment, it is believed that the complementarity of the targeting domain with the target sequence contributes to specificity of the interaction of the gRNA molecule/Cas9 molecule complex with a target nucleic acid. It is understood that in a targeting domain and target sequence pair, the uracil bases in the targeting domain will pair with the adenine bases in the target sequence. In an embodiment, the target domain itself comprises in the 5' to 3' direction, an optional secondary domain, and a core domain. In an embodiment, the core domain is fully complementary with the target sequence. In an embodiment, the targeting domain is 5 to 50 nucleotides in length. The strand of the target nucleic acid with which the targeting domain is complementary is referred to herein as the complementary strand. Some or all of the nucleotides of the domain can have a modification, e.g., a modification found in Section VIII herein.

In an embodiment, the targeting domain is 16 nucleotides in length.

In an embodiment, the targeting domain is 17 nucleotides in length.

In an embodiment, the targeting domain is 18 nucleotides in length.

In an embodiment, the targeting domain is 19 nucleotides in length.

In an embodiment, the targeting domain is 20 nucleotides in length.

In an embodiment, the targeting domain is 21 nucleotides in length.

In an embodiment, the targeting domain is 22 nucleotides in length.

In an embodiment, the targeting domain is 23 nucleotides in length.

In an embodiment, the targeting domain is 24 nucleotides in length.

In an embodiment, the targeting domain is 25 nucleotides in length.

In an embodiment, the targeting domain is 26 nucleotides in length.

In an embodiment, the targeting domain comprises 16 nucleotides.

In an embodiment, the targeting domain comprises 17 nucleotides.

In an embodiment, the targeting domain comprises 18 nucleotides.

In an embodiment, the targeting domain comprises 19 nucleotides.

In an embodiment, the targeting domain comprises 20 nucleotides.

In an embodiment, the targeting domain comprises 21 nucleotides.

In an embodiment, the targeting domain comprises 22 nucleotides.

In an embodiment, the targeting domain comprises 23 nucleotides.

In an embodiment, the targeting domain comprises 24 nucleotides.

In an embodiment, the targeting domain comprises 25 nucleotides.

In an embodiment, the targeting domain comprises 26 nucleotides.

Targeting domains are discussed in more detail below.

The First Complementarity Domain

Figs. 1A-1G provides examples of first complementarity domains.

The first complementarity domain is complementary with the second

complementarity domain, and in an embodiment, has sufficient complementarity to the second complementarity domain to form a duplexed region under at least some physiological conditions. In an embodiment, the first complementarity domain is 5 to 30 nucleotides in length. In an embodiment, the first complementarity domain is 5 to 25 nucleotides in length. In an embodiment, the first complementary domain is 7 to 25 nucleotides in length. In an embodiment, the first complementary domain is 7 to 22 nucleotides in length. In an embodiment, the first complementary domain is 7 to 18 nucleotides in length. In an embodiment, the first complementary domain is 7 to 15 nucleotides in length. In an embodiment, the first complementary domain is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotides in length.

In an embodiment, the first complementarity domain comprises 3 subdomains, which, in the 5' to 3' direction are: a 5' subdomain, a central subdomain, and a 3' subdomain. In an embodiment, the 5' subdomain is 4 to 9, e.g., 4, 5, 6, 7, 8 or 9 nucleotides in length. In an embodiment, the central subdomain is 1, 2, or 3, e.g., 1, nucleotide in length. In an embodiment, the 3' subdomain is 3 to 25, e.g., 4 to22, 4 to 18, or 4 to 10, or 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotides in length.

The first complementarity domain can share homology with, or be derived from, a naturally occurring first complementarity domain. In an embodiment, it has at least 50% homology with a first complementarity domain disclosed herein, e.g., an S. pyogenes, S. aureus or S. thermophilus, first complementarity domain.

Some or all of the nucleotides of the domain can have a modification, e.g., a modification found in Section VIII herein.

First complementarity domains are discussed in more detail below.

The Linking Domain

Figs. 1A-1G provides example of linking domains.

A linking domain serves to link the first complementarity domain with the second complementarity domain of a unimolecular gRNA. The linking domain can link the first and second complementarity domains covalently or non-covalently. In an embodiment, the linkage is covalent. In an embodiment, the linking domain covalently couples the first and second complementarity domains, see, e.g., Figs. IB-IE. In an embodiment, the linking domain is, or comprises, a covalent bond interposed between the first complementarity domain and the second complementarity domain. Typically the linking domain comprises one or more, e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides.

In modular gRNA molecules the two molecules are associated by virtue of the hybridization of the complementarity domains see e.g., Figs. 1A.

A wide variety of linking domains are suitable for use in unimolecular gRNA molecules. Linking domains can consist of a covalent bond, or be as short as one or a few nucleotides, e.g., 1, 2, 3, 4, or 5 nucleotides in length. In an embodiment, a linking domain is 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25 or more nucleotides in length. In an embodiment, a linking domain is 2 to 50, 2 to 40, 2 to 30, 2 to 20, 2 to 10, or 2 to 5 nucleotides in length. In an embodiment, a linking domain shares homology with, or is derived from, a naturally occurring sequence, e.g., the sequence of a tracrRNA that is 5' to the second complementarity domain. In an embodiment, the linking domain has at least 50% homology with a linking domain disclosed herein.

Some or all of the nucleotides of the domain can have a modification, e.g., a modification found in Section VIII herein.

Linking domains are discussed in more detail below.

The 5 ' Extension Domain

In an embodiment, a modular gRNA can comprise additional sequence, 5' to the second complementarity domain, referred to herein as the 5' extension domain, see, e.g., Fig. 1A. In an embodiment, the 5' extension domain is, 2 to 10, 2 to 9, 2 to 8, 2 to 7, 2 to 6, 2 to 5, or 2 to 4 nucleotides in length. In an embodiment, the 5' extension domain is 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more nucleotides in length.

The Second Complementarity Domain

Figs. 1A-1G provide examples of second complementarity domains.

The second complementarity domain is complementary with the first

complementarity domain, and in an embodiment, has sufficient complementarity to the second complementarity domain to form a duplexed region under at least some physiological conditions. In an embodiment, e.g., as shown in Figs. 1A-1B, the second complementarity domain can include sequence that lacks complementarity with the first complementarity domain, e.g., sequence that loops out from the duplexed region.

In an embodiment, the second complementarity domain is 5 to 27 nucleotides in length. In an embodiment, it is longer than the first complementarity region. In an embodiment the second complementary domain is 7 to 27 nucleotides in length. In an embodiment, the second complementary domain is 7 to 25 nucleotides in length. In an embodiment, the second complementary domain is 7 to 20 nucleotides in length. In an embodiment, the second complementary domain is 7 to 17 nucleotides in length. In an embodiment, the complementary domain is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 nucleotides in length. In an embodiment, the second complementarity domain comprises 3 subdomains, which, in the 5' to 3' direction are: a 5' subdomain, a central subdomain, and a 3' subdomain. In an embodiment, the 5' subdomain is 3 to 25, e.g., 4 to 22, 4 tol8, or 4 to 10, or 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotides in length. In an embodiment, the central subdomain is 1, 2, 3, 4 or 5, e.g., 3, nucleotides in length. In an embodiment, the 3' subdomain is 4 to 9, e.g., 4, 5, 6, 7, 8 or 9 nucleotides in length.

In an embodiment, the 5 ' subdomain and the 3 ' subdomain of the first

complementarity domain, are respectively, complementary, e.g., fully complementary, with the 3' subdomain and the 5' subdomain of the second complementarity domain.

The second complementarity domain can share homology with or be derived from a naturally occurring second complementarity domain. In an embodiment, it has at least 50% homology with a second complementarity domain disclosed herein, e.g., an S. pyogenes, S. aureus or S. thermophilus, first complementarity domain.

Some or all of the nucleotides of the domain can have a modification, e.g., a modification found in Section VIII herein.

A Proximal domain

Figs. 1A-1G provide examples of proximal domains.

In an embodiment, the proximal domain is 5 to 20 nucleotides in length. In an embodiment, the proximal domain can share homology with or be derived from a naturally occurring proximal domain. In an embodiment, it has at least 50% homology with a proximal domain disclosed herein, e.g., an S. pyogenes, S. aureus or S. thermophilus, proximal domain.

Some or all of the nucleotides of the domain can have a modification, e.g., modification found in Section VIII herein.

A Tail Domain

Figs. 1A-1G provide examples of tail domains.

As can be seen by inspection of the tail domains in Figs. 1A-1E, a broad spectrum of tail domains are suitable for use in gRNA molecules. In an embodiment, the tail domain is 0 (absent), 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length. In embodiment, the tail domain nucleotides are from or share homology with sequence from the 5 ' end of a naturally occurring tail domain, see e.g., Fig. ID or Fig.lE. In an embodiment, the tail domain includes sequences that are complementary to each other and which, under at least some physiological conditions, form a duplexed region.

In an embodiment, the tail domain is absent or is 1 to 50 nucleotides in length. In an embodiment, the tail domain can share homology with or be derived from a naturally occurring proximal tail domain. In an embodiment, it has at least 50% homology with a tail domain disclosed herein, e.g., an S. pyogenes, S. aureus or S. thermophilus, tail domain.

In an embodiment, the tail domain includes nucleotides at the 3' end that are related to the method of in vitro or in vivo transcription. When a T7 promoter is used for in vitro transcription of the gRNA, these nucleotides may be any nucleotides present before the 3 ' end of the DNA template. When a U6 promoter is used for in vivo transcription, these nucleotides may be the sequence UUUUUU. When alternate pol-III promoters are used, these nucleotides may be various numbers or uracil bases or may include alternate bases.

The domains of gRNA molecules are described in more detail below. The Targeting Domain

The "targeting domain" of the gRNA is complementary to the "target domain" on the target nucleic acid. The strand of the target nucleic acid comprising the nucleotide sequence complementary to the core domain of the gRNA is referred to herein as the "complementary strand" of the target nucleic acid. Guidance on the selection of targeting domains can be found, e.g., in Fu Y et al., Nat Biotechnol 2014 (doi: 10.1038/nbt.2808) and Sternberg SH et al., Nature 2014 (doi: 10.1038/naturel3011).

In an embodiment, the targeting domain is 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, the targeting domain is 16 nucleotides in length.

In an embodiment, the targeting domain is 17 nucleotides in length.

In an embodiment, the targeting domain is 18 nucleotides in length.

In an embodiment, the targeting domain is 19 nucleotides in length.

In an embodiment, the targeting domain is 20 nucleotides in length.

In an embodiment, the targeting domain is 21 nucleotides in length.

In an embodiment, the targeting domain is 22 nucleotides in length.

In an embodiment, the targeting domain is 23 nucleotides in length.

In an embodiment, the targeting domain is 24 nucleotides in length.

In an embodiment, the targeting domain is 25 nucleotides in length.

In an embodiment, the targeting domain is 26 nucleotides in length. In an embodiment, the targeting domain comprises 16 nucleotides.

In an embodiment, the targeting domain comprises 17 nucleotides.

In an embodiment, the targeting domain comprises 18 nucleotides.

In an embodiment, the targeting domain comprises 19 nucleotides.

In an embodiment, the targeting domain comprises 20 nucleotides.

In an embodiment, the targeting domain comprises 21 nucleotides.

In an embodiment, the targeting domain comprises 22 nucleotides.

In an embodiment, the targeting domain comprises 23 nucleotides.

In an embodiment, the targeting domain comprises 24 nucleotides.

In an embodiment, the targeting domain comprises 25 nucleotides.

In an embodiment, the targeting domain comprises 26 nucleotides.

In an embodiment, the targeting domain is 10 +/-5, 20+/-5, 30+/-5,

60+/-5, 70+/-5, 80+/-5, 90+/-5, or 100+/-5 nucleotides, in length.

In an embodiment, the targeting domain is 20+/-5 nucleotides in length.

In an embodiment, the targeting domain is 20+/- 10, 30+/- 10, 40+/- 10, 50+/- 10, 60+/-

10, 70+/-10, 80+/-10, 90+/-10, or 100+/- 10 nucleotides, in length.

In an embodiment, the targeting domain is 30+/- 10 nucleotides in length.

In an embodiment, the targeting domain is 10 to 100, 10 to 90, 10 to 80, 10 to 70, 10 to 60, 10 to 50, 10 to 40, 10 to 30, 10 to 20 or 10 to 15 nucleotides in length. In another embodiment, the targeting domain is 20 to 100, 20 to 90, 20 to 80, 20 to 70, 20 to 60, 20 to 50, 20 to 40, 20 to 30, or 20 to 25 nucleotides in length.

Typically the targeting domain has full complementarity with the target sequence. In an embodiment the targeting domain has or includes 1, 2, 3, 4, 5, 6, 7 or 8 nucleotides that are not complementary with the corresponding nucleotide of the targeting domain.

In an embodiment, the target domain includes 1, 2, 3, 4 or 5 nucleotides that are complementary with the corresponding nucleotide of the targeting domain within 5 nucleotides of its 5' end. In an embodiment, the target domain includes 1, 2, 3, 4 or 5 nucleotides that are complementary with the corresponding nucleotide of the targeting domain within 5 nucleotides of its 3 ' end.

In an embodiment, the target domain includes 1, 2, 3, or 4 nucleotides that are not complementary with the corresponding nucleotide of the targeting domain within 5 nucleotides of its 5' end. In an embodiment, the target domain includes 1, 2, 3, or 4 nucleotides that are not complementary with the corresponding nucleotide of the targeting domain within 5 nucleotides of its 3 ' end. In an embodiment, the degree of complementarity, together with other properties of the gRNA, is sufficient to allow targeting of a Cas9 molecule to the target nucleic acid.

In some embodiments, the targeting domain comprises two consecutive nucleotides that are not complementary to the target domain ("non-complementary nucleotides"), e.g., two consecutive noncomplementary nucleotides that are within 5 nucleotides of the 5' end of the targeting domain, within 5 nucleotides of the 3 ' end of the targeting domain, or more than 5 nucleotides away from one or both ends of the targeting domain.

In an embodiment, no two consecutive nucleotides within 5 nucleotides of the 5 ' end of the targeting domain, within 5 nucleotides of the 3 ' end of the targeting domain, or within a region that is more than 5 nucleotides away from one or both ends of the targeting domain, are not complementary to the targeting domain.

In an embodiment, there are no noncomplementary nucleotides within 5 nucleotides of the 5' end of the targeting domain, within 5 nucleotides of the 3' end of the targeting domain, or within a region that is more than 5 nucleotides away from one or both ends of the targeting domain.

In an embodiment, the targeting domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the targeting domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the targeting domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment, a nucleotide of the targeting domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other modification(s) from Section VIII.

In some embodiments, the targeting domain includes 1, 2, 3, 4, 5, 6, 7 or 8 or more modifications. In an embodiment, the targeting domain includes 1, 2, 3, or 4 modifications within 5 nucleotides of its 5' end. In an embodiment, the targeting domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 3' end.

In some embodiments, the targeting domain comprises modifications at two consecutive nucleotides, e.g., two consecutive nucleotides that are within 5 nucleotides of the 5' end of the targeting domain, within 5 nucleotides of the 3' end of the targeting domain, or more than 5 nucleotides away from one or both ends of the targeting domain.

In an embodiment, no two consecutive nucleotides are modified within 5 nucleotides of the 5' end of the targeting domain, within 5 nucleotides of the 3' end of the targeting domain, or within a region that is more than 5 nucleotides away from one or both ends of the targeting domain. In an embodiment, no nucleotide is modified within 5 nucleotides of the 5' end of the targeting domain, within 5 nucleotides of the 3' end of the targeting domain, or within a region that is more than 5 nucleotides away from one or both ends of the targeting domain.

Modifications in the targeting domain can be selected so as to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate targeting domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in a system in Section IV. The candidate targeting domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, all of the modified nucleotides are complementary to and capable of hybridizing to corresponding nucleotides present in the target domain. In another embodiment, 1, 2, 3, 4, 5, 6, 7 or 8 or more modified nucleotides are not complementary to or capable of hybridizing to corresponding nucleotides present in the target domain.

In an embodiment, the targeting domain comprises, preferably in the 5'→3' direction: a secondary domain and a core domain. These domains are discussed in more detail below.

The Core Domain and Secondary Domain of the Targeting Domain

The "core domain" of the targeting domain is complementary to the "core domain target" on the target nucleic acid. In an embodiment, the core domain comprises about 8 to about 13 nucleotides from the 3' end of the targeting domain (e.g., the most 3' 8 to 13 nucleotides of the targeting domain).

In an embodiment, the core domain and targeting domain, are independently, 6 +1-2, 7+/-2, 8+/-2, 9+/-2, 10+/-2, 11+/-2, 12+/-2, 13+/-2, 14+/-2, 15+/-2, or 16+-2, nucleotides in length.

In an embodiment, the core domain and targeting domain, are independently, 10+/-2 nucleotides in length.

In an embodiment, the core domain and targeting domain, are independently, 10+/-4 nucleotides in length.

In an embodiment, the core domain and targeting domain are independently 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 nucleotides in length.

In an embodiment, the core domain and targeting domain are independently 3 to 20, 4 to 20, 5 to 20, 6 to 20, 7 to 20, 8 to 20, 9 to 20 10 to 20 or 15 to 20 nucleotides in length. In an embodiment, the core domain and targeting domain are independently 3 to 15, e.g., 6 to 15, 7 to 14, 7 to 13, 6 to 12, 7 to 12, 7 to 11, 7 to 10, 8 to 14, 8 to 13, 8 to 12, 8 to 11, 8 to 10 or 8 to 9 nucleotides in length.

The core domain is complementary with the core domain target. Typically the core domain has exact complementarity with the core domain target. In some embodiments, the core domain can have 1, 2, 3, 4 or 5 nucleotides that are not complementary with the corresponding nucleotide of the core domain. In an embodiment, the degree of

complementarity, together with other properties of the gRNA, is sufficient to allow targeting of a Cas9 molecule to the target nucleic acid.

The "secondary domain" of the targeting domain of the gRNA is complementary to the "secondary domain target" of the target nucleic acid.

In an embodiment, the secondary domain is positioned 5 ' to the core domain.

In an embodiment, the secondary domain is absent or optional.

In an embodiment, if the targeting domain is 26 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 12 to 17 nucleotides in length.

In an embodiment, if the targeting domain is 25 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 12 to 17 nucleotides in length.

In an embodiment, if the targeting domain is 24 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 11 to 16 nucleotides in length.

In an embodiment, if the targeting domain is 23 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 10 to 15 nucleotides in length.

In an embodiment, if the targeting domain is 22 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 9 to 14 nucleotides in length.

In an embodiment, if the targeting domain is 21 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 8 to 13 nucleotides in length.

In an embodiment, if the targeting domain is 20 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 7 to 12 nucleotides in length. In an embodiment, if the targeting domain is 19 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 6 to 11 nucleotides in length.

In an embodiment, if the targeting domain is 18 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 5 to 10 nucleotides in length.

In an embodiment, if the targeting domain is 17 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 4 to 9 nucleotides in length.

In an embodiment, if the targeting domain is 16 nucleotides in length and the core domain (counted from the 3' end of the targeting domain) is 8 to 13 nucleotides in length, the secondary domain is 3 to 8 nucleotides in length.

In an embodiment, the secondary domain is 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 nucleotides in length.

The secondary domain is complementary with the secondary domain target.

Typically the secondary domain has exact complementarity with the secondary domain target. In some embodiments the secondary domain can have 1, 2, 3, 4 or 5 nucleotides that are not complementary with the corresponding nucleotide of the secondary domain. In an embodiment, the degree of complementarity, together with other properties of the gRNA, is sufficient to allow targeting of a Cas9 molecule to the target nucleic acid.

In an embodiment, the core domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the core domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the core domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the core domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other

modification(s) from Section VIII. Typically, a core domain will contain no more than 1, 2, or 3 modifications.

Modifications in the core domain can be selected so as to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate core domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described at Section IV. The candidate core domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the secondary domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the secondary domain comprises one or more modifications, e.g., modifications that render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the secondary domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the secondary domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other modification(s) from Section VIII. Typically, a secondary domain will contain no more than 1, 2, or 3 modifications.

Modifications in the secondary domain can be selected to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate secondary domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described at Section IV. The candidate secondary domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, (1) the degree of complementarity between the core domain and its target, and (2) the degree of complementarity between the secondary domain and its target, may differ. In an embodiment, (1) may be greater than (2). In an embodiment, (1) may be less than (2). In an embodiment, (1) and (2) are the same, e.g., each may be completely complementary with its target.

In an embodiment, (1) the number of modifications (e.g., modifications from Section VIII) of the nucleotides of the core domain and (2) the number of modifications (e.g., modifications from Section VIII) of the nucleotides of the secondary domain may differ. In an embodiment, (1) may be less than (2). In an embodiment, (1) may be greater than (2). In an embodiment, (1) and (2) may be the same, e.g., each may be free of modifications.

The First and Second Complementarity Domains

The first complementarity domain is complementary with the second

complementarity domain.

Typically the first domain does not have exact complementarity with the second complementarity domain target. In some embodiments, the first complementarity domain can have 1, 2, 3, 4 or 5 nucleotides that are not complementary with the corresponding nucleotide of the second complementarity domain. In an embodiment, 1, 2, 3, 4, 5 or 6, e.g., 3 nucleotides, will not pair in the duplex, and, e.g., form a non-duplexed or looped-out region. In an embodiment, an unpaired, or loop-out, region, e.g., a loop-out of 3 nucleotides, is present on the second complementarity domain. In an embodiment, the unpaired region begins 1 , 2, 3, 4, 5, or 6, e.g., 4, nucleotides from the 5 ' end of the second complementarity domain.

In an embodiment, the degree of complementarity, together with other properties of the gRNA, is sufficient to allow targeting of a Cas9 molecule to the target nucleic acid.

In an embodiment, the first and second complementarity domains are:

independently, 6 +1-2, Ί+Ι-2, 8+/-2, 9+/-2, 10+/-2, 11+/-2, 12+/-2, 13+/-2, 14+/-2, 15+/-2, 16+/-2, 17+/-2, 18+/-2, 19+/-2, or 20+/-2, 21+/-2, 22+/-2, 23+/-2, or 24+/-2 nucleotides in length;

independently, 6, 7, 8, 9, 10, 11, 12, 13, 14, 14, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, or 26 nucleotides in length; or

independently, 5 to 24, 5 to 23, 5 to 22, 5 to 21, 5 to 20, 7 to 18, 9 to 16, or 10 to 14 nucleotides in length.

In an embodiment, the second complementarity domain is longer than the first complementarity domain, e.g., 2, 3, 4, 5, or 6, e.g., 6, nucleotides longer.

In an embodiment, the first and second complementary domains, independently, do not comprise modifications, e.g., modifications of the type provided in Section VIII.

In an embodiment, the first and second complementary domains, independently, comprise one or more modifications, e.g., modifications that the render the domain less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other

modification(s) from Section VIII.

In an embodiment, the first and second complementary domains, independently, include 1 , 2, 3, 4, 5, 6, 7 or 8 or more modifications. In an embodiment, the first and second complementary domains, independently, include 1, 2, 3, or 4 modifications within 5 nucleotides of its 5' end. In an embodiment, the first and second complementary domains, independently, include as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 3' end. In an embodiment, the first and second complementary domains, independently, include modifications at two consecutive nucleotides, e.g., two consecutive nucleotides that are within 5 nucleotides of the 5 ' end of the domain, within 5 nucleotides of the 3 ' end of the domain, or more than 5 nucleotides away from one or both ends of the domain. In an embodiment, the first and second complementary domains, independently, include no two consecutive nucleotides that are modified, within 5 nucleotides of the 5' end of the domain, within 5 nucleotides of the 3' end of the domain, or within a region that is more than 5 nucleotides away from one or both ends of the domain. In an embodiment, the first and second complementary domains, independently, include no nucleotide that is modified within 5 nucleotides of the 5' end of the domain, within 5 nucleotides of the 3' end of the domain, or within a region that is more than 5 nucleotides away from one or both ends of the domain.

Modifications in a complementarity domain can be selected so as to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate complementarity domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described in Section IV. The candidate complementarity domain can be placed, either alone, or with one or more other candidate changes in a gRNA

molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the first complementarity domain has at least 60, 70, 80, 85%, 90% or 95% homology with, or differs by no more than 1, 2, 3, 4, 5, or 6 nucleotides from, a reference first complementarity domain, e.g., a naturally occurring, e.g., an S. pyogenes, S. aureus or S. thermophilus, first complementarity domain, or a first complementarity domain described herein, e.g., from Figs. 1A-1G.

In an embodiment, the second complementarity domain has at least 60, 70, 80, 85%, 90%, or 95% homology with, or differs by no more than 1, 2, 3, 4, 5, or 6 nucleotides from, a reference second complementarity domain, e.g., a naturally occurring, e.g., an S. pyogenes, S. aureus or S. thermophilus, second complementarity domain, or a second complementarity domain described herein, e.g., from Figs. 1A-1G.

The duplexed region formed by first and second complementarity domains is typically 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 or 22 base pairs in length (excluding any looped out or unpaired nucleotides).

In some embodiments, the first and second complementarity domains, when duplexed, comprise 11 paired nucleotides, for example, in the gRNA sequence (one paired strand underlined, one bolded): NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAA GGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC (SEQ ID NO: 5).

In some embodiments, the first and second complementarity domains, when duplexed, comprise 15 paired nucleotides, for example in the gRNA sequence (one paired strand underlined, one bolded):

NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAUGCUGAAAAGCAUAGCAAGU UAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC

(SEQ ID NO: 27).

In some embodiments the first and second complementarity domains, when duplexed, comprise 16 paired nucleotides, for example in the gRNA sequence (one paired strand underlined, one bolded):

NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAUGCUGGAAACAGCAUAGCAA GUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGU GC (SEQ ID NO: 28).

In some embodiments the first and second complementarity domains, when duplexed, comprise 21 paired nucleotides, for example in the gRNA sequence (one paired strand underlined, one bolded):

NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAUGCUGUUUUGGAAACAAAAC AGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCA CCGAGUCGGUGC (SEQ ID NO: 29).

In some embodiments, nucleotides are exchanged to remove poly-U tracts, for example in the gRNA sequences (exchanged nucleotides underlined):

NNNNNNNNNNNNNNNNNNNNGUAUUAGAGCUAGAAAUAGCAAGUUAAUAUAA GGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC (SEQ ID NO: 30);

NNNNNNNNNNNNNNNNNNNNGUUUAAGAGCUAGAAAUAGCAAGUUUAAAUAA GGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC (SEQ ID NO: 31); or

NNNNNNNNNNNNNNNNNNNNGUAUUAGAGCUAUGCUGUAUUGGAAACAAUAC AGCAUAGCAAGUUAAUAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCA CCGAGUCGGUGC (SEQ ID NO: 32). The 5 ' Extension Domain

In an embodiment, a modular gRNA can comprise additional sequence, 5' to the second complementarity domain. In an embodiment, the 5' extension domain is 2 to 10, 2 to 9, 2 to 8, 2 to 7, 2 to 6, 2 to 5, or 2 to 4 nucleotides in length. In an embodiment, the 5' extension domain is 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more nucleotides in length.

In an embodiment, the 5 ' extension domain nucleotides do not comprise

modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the 5' extension domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the 5' extension domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment, a nucleotide of the 5' extension domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other modification(s) from Section VIII.

In some embodiments, the 5' extension domain can comprise as many as 1, 2, 3, 4, 5, 6, 7 or 8 modifications. In an embodiment, the 5' extension domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 5' end, e.g., in a modular gRNA molecule. In an embodiment, the 5' extension domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 3' end, e.g., in a modular gRNA molecule.

In some embodiments, the 5' extension domain comprises modifications at two consecutive nucleotides, e.g., two consecutive nucleotides that are within 5 nucleotides of the 5' end of the 5' extension domain, within 5 nucleotides of the 3' end of the 5' extension domain, or more than 5 nucleotides away from one or both ends of the 5' extension domain. In an embodiment, no two consecutive nucleotides are modified within 5 nucleotides of the 5' end of the 5' extension domain, within 5 nucleotides of the 3' end of the 5' extension domain, or within a region that is more than 5 nucleotides away from one or both ends of the 5' extension domain. In an embodiment, no nucleotide is modified within 5 nucleotides of the 5 ' end of the 5 ' extension domain, within 5 nucleotides of the 3 ' end of the 5 ' extension domain, or within a region that is more than 5 nucleotides away from one or both ends of the 5' extension domain.

Modifications in the 5 ' extension domain can be selected so as to not interfere with gRNA molecule efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate 5 ' extension domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described at Section IV. The candidate 5 ' extension domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the 5' extension domain has at least 60, 70, 80, 85, 90 or 95% homology with, or differs by no more than 1, 2, 3, 4, 5, or 6 nucleotides from, a reference 5' extension domain, e.g., a naturally occurring, e.g., an S. pyogenes, S. aureus or S.

thermophilus, 5' extension domain, or a 5' extension domain described herein, e.g., from Figs. 1A-1G.

The Linking Domain

In a unimolecular gRNA molecule the linking domain is disposed between the first and second complementarity domains. In a modular gRNA molecule, the two molecules are associated with one another by the complementarity domains.

In an embodiment, the linking domain is 10 +/-5, 20+/-5, 30+/-5, 40+/-5, 50+/-5, 60+/-5, 70+/-5, 80+/-5, 90+/-5, or 100+/-5 nucleotides, in length.

In an embodiment, the linking domain is 20+/-10, 30+/-10, 40+/-10, 50+/-10, 60+/-

10, 70+/-10, 80+/-10, 90+/-10, or 100+/- 10 nucleotides, in length.

In an embodiment, the linking domain is 10 to 100, 10 to 90, 10 to 80, 10 to 70, 10 to 60, 10 to 50, 10 to 40, 10 to 30, 10 to 20 or 10 to 15 nucleotides in length. In other embodiments, the linking domain is 20 to 100, 20 to 90, 20 to 80, 20 to 70, 20 to 60, 20 to 50, 20 to 40, 20 to 30, or 20 to 25 nucleotides in length.

In an embodiment, the linking domain is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 17, 18, 19, or 20 nucleotides in length.

In and embodiment, the linking domain is a covalent bond.

In an embodiment, the linking domain comprises a duplexed region, typically adjacent to or within 1, 2, or 3 nucleotides of the 3' end of the first complementarity domain and/or the 5- end of the second complementarity domain. In an embodiment, the duplexed region can be 20+/- 10 base pairs in length. In an embodiment, the duplexed region can be 10+/-5, 15+/-5, 20+/-5, or 30+/-5 base pairs in length. In an embodiment, the duplexed region can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 base pairs in length.

Typically the sequences forming the duplexed region have exact complementarity with one another, though in some embodiments as many as 1, 2, 3, 4, 5, 6, 7 or 8 nucleotides are not complementary with the corresponding nucleotides.

In an embodiment, the linking domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the linking domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the linking domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the linking domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other modification(s) from Section VIII. In an embodiment, the linking domain can comprise as many as 1, 2, 3, 4, 5, 6, 7 or 8 modifications.

Modifications in a linking domain can be selected so as to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate linking domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated a system described in Section IV. A candidate linking domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the linking domain has at least 60, 70, 80, 85, 90 or 95% homology with, or differs by no more than 1, 2, 3, 4, 5 , or 6 nucleotides from, a reference linking domain, e.g., a linking domain described herein, e.g., from Figs. 1A-1G.

The Proximal Domain

In an embodiment, the proximal domain is 6 +1-2, 1+1-2, 8+/-2, 9+/-2, 10+/-2, 11+/-2,

12+/-2, 13+/-2, 14+/-2, 14+/-2, 16+/-2, 17+/-2, 18+/-2, 19+/-2, or 20+/-2 nucleotides in length.

In an embodiment, the proximal domain is 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, the proximal domain is 5 to 20, 7, to 18, 9 to 16, or 10 to 14 nucleotides in length.

In an embodiment, the proximal domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the proximal domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the proximal domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the proximal domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other modification(s) from Section VIII. In some embodiments, the proximal domain can comprise as many as 1, 2, 3, 4, 5, 6, 7 or 8 modifications. In an embodiment, the proximal domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 5' end, e.g., in a modular gRNA molecule. In an embodiment, the target domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 3' end, e.g., in a modular gRNA molecule.

In some embodiments, the proximal domain comprises modifications at two consecutive nucleotides, e.g., two consecutive nucleotides that are within 5 nucleotides of the 5 ' end of the proximal domain, within 5 nucleotides of the 3 ' end of the proximal domain, or more than 5 nucleotides away from one or both ends of the proximal domain. In an embodiment, no two consecutive nucleotides are modified within 5 nucleotides of the 5' end of the proximal domain, within 5 nucleotides of the 3 ' end of the proximal domain, or within a region that is more than 5 nucleotides away from one or both ends of the proximal domain. In an embodiment, no nucleotide is modified within 5 nucleotides of the 5 ' end of the proximal domain, within 5 nucleotides of the 3' end of the proximal domain, or within a region that is more than 5 nucleotides away from one or both ends of the proximal domain.

Modifications in the proximal domain can be selected to not interfere with gRNA molecule efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate proximal domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described at Section IV. The candidate proximal domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the proximal domain has at least 60, 70, 80, 85 90 or 95% homology with, or differs by no more than 1, 2, 3, 4, 5 , or 6 nucleotides from, a reference proximal domain, e.g., a naturally occurring, e.g., an S. pyogenes, S. aureus or S.

thermophilus, proximal domain, or a proximal domain described herein, e.g., from Figs. 1A- 1G.

The Tail Domain

In an embodiment, the tail domain is 10 +/-5, 20+/-5, 30+/-5, 40+/-5, 50+/-5, 60+/-5, 70+/-5, 80+/-5, 90+/-5, or 100+/-5 nucleotides, in length.

In an embodiment, the tail domain is 20+/-5 nucleotides in length.

In an embodiment, the tail domain is 20+/-10, 30+/-10, 40+/-10, 50+/-10, 60+/-10, 70+/- 10, 80+/- 10, 90+/- 10, or 100+/- 10 nucleotides, in length. In an embodiment, the tail domain is 25+/- 10 nucleotides in length.

In an embodiment, the tail domain is 10 to 100, 10 to 90, 10 to 80, 10 to 70, 10 to 60, 10 to 50, 10 to 40, 10 to 30, 10 to 20 or 10 to 15 nucleotides in length.

In other embodiments, the tail domain is 20 to 100, 20 to 90, 20 to 80, 20 to 70, 20 to 60, 20 to 50, 20 to 40, 20 to 30, or 20 to 25 nucleotides in length.

In an embodiment, the tail domain is 1 to 20, 1 to 15, 1 to 10, or 1 to 5 nucleotides in length.

In an embodiment, the tail domain nucleotides do not comprise modifications, e.g., modifications of the type provided in Section VIII. However, in an embodiment, the tail domain comprises one or more modifications, e.g., modifications that it render it less susceptible to degradation or more bio-compatible, e.g., less immunogenic. By way of example, the backbone of the tail domain can be modified with a phosphorothioate, or other modification(s) from Section VIII. In an embodiment a nucleotide of the tail domain can comprise a 2' modification, e.g., a 2-acetylation, e.g., a 2' methylation, or other

modification(s) from Section VIII.

In some embodiments, the tail domain can have as many as 1, 2, 3, 4, 5, 6, 7 or 8 modifications. In an embodiment, the target domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 5' end. In an embodiment, the target domain comprises as many as 1, 2, 3, or 4 modifications within 5 nucleotides of its 3' end.

In an embodiment, the tail domain comprises a tail duplex domain, which can form a tail duplexed region. In an embodiment, the tail duplexed region can be 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 base pairs in length. In an embodiment, a further single stranded domain, exists 3' to the tail duplexed domain. In an embodiment, this domain is 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length. In an embodiment it is 4 to 6 nucleotides in length.

In an embodiment, the tail domain has at least 60, 70, 80, or 90% homology with, or differs by no more than 1, 2, 3, 4, 5 ,or 6 nucleotides from, a reference tail domain, e.g., a naturally occurring, e.g., an S. pyogenes, S. aureus or S. thermophilus, tail domain, or a tail domain described herein, e.g., from Figs. 1A-1G.

In an embodiment, the proximal and tail domain, taken together comprise the following sequences:

AAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCU (SEQ ID NO: 33), or

AAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGGUGC (SEQ ID NO: 34), or AAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCGGAUC (SEQ ID NO: 35), or

AAGGCUAGUCCGUUAUCAACUUGAAAAAGUG (SEQ ID NO: 36), or

AAGGCUAGUCCGUUAUCA (SEQ ID NO: 37), or

AAGGCUAGUCCG (SEQ ID NO: 38).

In an embodiment, the tail domain comprises the 3' sequence UUUUUU, e.g., if a U6 promoter is used for transcription.

In an embodiment, the tail domain comprises the 3' sequence UUUU, e.g., if an HI promoter is used for transcription.

In an embodiment, tail domain comprises variable numbers of 3' Us depending, e.g., on the termination signal of the pol-III promoter used.

In an embodiment, the tail domain comprises variable 3 ' sequence derived from the DNA template if a T7 promoter is used.

In an embodiment, the tail domain comprises variable 3' sequence derived from the

DNA template, e.g., if in vitro transcription is used to generate the RNA molecule.

In an embodiment, the tail domain comprises variable 3 ' sequence derived from the DNA template, e., if a pol-II promoter is used to drive transcription.

Modifications in the tail domain can be selected so as to not interfere with targeting efficacy, which can be evaluated by testing a candidate modification in the system described in Section IV. gRNAs having a candidate tail domain having a selected length, sequence, degree of complementarity, or degree of modification, can be evaluated in the system described in Section IV. The candidate tail domain can be placed, either alone, or with one or more other candidate changes in a gRNA molecule/Cas9 molecule system known to be functional with a selected target and evaluated.

In an embodiment, the tail domain comprises modifications at two consecutive nucleotides, e.g., two consecutive nucleotides that are within 5 nucleotides of the 5' end of the tail domain, within 5 nucleotides of the 3' end of the tail domain, or more than 5 nucleotides away from one or both ends of the tail domain. In an embodiment, no two consecutive nucleotides are modified within 5 nucleotides of the 5' end of the tail domain, within 5 nucleotides of the 3' end of the tail domain, or within a region that is more than 5 nucleotides away from one or both ends of the tail domain. In an embodiment, no nucleotide is modified within 5 nucleotides of the 5 ' end of the tail domain, within 5 nucleotides of the 3' end of the tail domain, or within a region that is more than 5 nucleotides away from one or both ends of the tail domain.

In an embodiment a gRNA has the following structure:

5' [targeting domain] -[first complementarity domain] -[linking domain] -[second complementarity domain] -[proximal domain]-[tail domain]-3'

wherein, the targeting domain comprises a core domain and optionally a secondary domain, and is 10 to 50 nucleotides in length;

the first complementarity domain is 5 to 25 nucleotides in length and, In an embodiment has at least 50, 60, 70, 80, 85, 90 or 95% homology with a reference first complementarity domain disclosed herein;

the linking domain is 1 to 5 nucleotides in length;

the second complementarity domain is 5 to 27 nucleotides in length and, in an embodiment has at least 50, 60, 70, 80, 85, 90 or 95% homology with a reference second complementarity domain disclosed herein;

the proximal domain is 5 to 20 nucleotides in length and, in an embodiment has at least 50, 60, 70, 80, 85, 90 or 95% homology with a reference proximal domain disclosed herein; and

the tail domain is absent or a nucleotide sequence is 1 to 50 nucleotides in length and, in an embodiment has at least 50, 60, 70, 80, 85, 90 or 95% homology with a reference tail domain disclosed herein.

Exemplary Chimeric gRNAs

In an embodiment, a unimolecular, or chimeric, gRNA comprises, preferably from 5' to 3':

a targeting domain (which is complementary to a target nucleic acid);

a first complementarity domain, e.g., comprising 15, 16, 17, 18, 19, 20, 21, 22,

23, 24, 25, or 26 nucleotides;

a linking domain;

a second complementarity domain (which is complementary to the first complementarity domain);

a proximal domain; and

a tail domain,

wherein,

(a) the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides;

(b) there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain; or

(c) there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the sequence from (a), (b), or (c), has at least 60, 75, 80, 85, 90, 95, or 99% homology with the corresponding sequence of a naturally occurring gRNA, or with a gRNA described herein.

In an embodiment, the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3 ' to the last nucleotide of the second complementarity domain.

In an embodiment, there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3 ' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides (e.g., 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides

(e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length. In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides

(e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and the proximal and tail domain, when taken together, comprise at leastl5, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides

(e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides

(e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides (e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain. In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides (e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the unimolecular, or chimeric, gRNA molecule (comprising a targeting domain, a first complementary domain, a linking domain, a second complementary domain, a proximal domain and, optionally, a tail domain) comprises the following sequence in which the targeting domain is depicted as 20 Ns but could be any sequence and range in length from 16 to 26 nucleotides and in which the gRNA sequence is followed by 6 Us, which serve as a termination signal for the U6 promoter, but which could be either absent or fewer in number:

NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAGAAAUAGCAAGUUAAA AUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU UU (SEQ ID NO: 45). In an embodiment, the unimolecular, or chimeric, gRNA molecule is a S. pyogenes gRNA molecule.

In some embodiments, the unimolecular, or chimeric, gRNA molecule (comprising a targeting domain, a first complementary domain, a linking domain, a second complementary domain, a proximal domain and, optionally, a tail domain) comprises the following sequence in which the targeting domain is depicted as 20 Ns but could be any sequence and range in length from 16 to 26 nucleotides and in which the gRNA sequence is followed by 6 Us, which serve as a termination signal for the U6 promoter, but which could be either absent or fewer in number:

NNNNNNNNNNNNNNNNNNNNGUUUUAGUACUCUGGAAACAGAAUCUAC UAAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGAUUUU UU (SEQ ID NO: 40). In an embodiment, the unimolecular, or chimeric, gRNA molecule is a S. aureus gRNA molecule.

Exemplary Modular gRNAs

In an embodiment, a modular gRNA comprises:

a first strand comprising, preferably from 5' to 3' ;

a targeting domain, e.g., comprising 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or 26 nucleotides;

a first complementarity domain; and

a second strand, comprising, preferably from 5 ' to 3 ' :

optionally a 5' extension domain;

a second complementarity domain;

a proximal domain; and

a tail domain,

wherein:

(a) the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides;

(b) there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain; or

(c) there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the sequence from (a), (b), or (c), has at least 60, 75, 80, 85, 90, 95, or 99% homology with the corresponding sequence of a naturally occurring gRNA, or with a gRNA described herein.

In an embodiment, the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3 ' to the last nucleotide of the second complementarity domain. In an embodiment, there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3 ' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides (e.g., 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides

(e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides (e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length. In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 16 nucleotides (e.g., 16 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 16 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 17 nucleotides (e.g., 17 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 17 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 18 nucleotides (e.g., 18 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 18 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 19 nucleotides (e.g., 19 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 19 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 20 nucleotides (e.g., 20 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 20 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 21 nucleotides (e.g., 21 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 21 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 22 nucleotides (e.g., 22 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 22 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides

(e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides (e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 23 nucleotides (e.g., 23 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 23 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides

(e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain. In an embodiment, the targeting domain comprises, has, or consists of, 24 nucleotides (e.g., 24 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 24 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 25 nucleotides (e.g., 25 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 25 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and there are at least 15, 18, 20, 25, 30, 31, 35,

40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain.

In an embodiment, the targeting domain comprises, has, or consists of, 26 nucleotides (e.g., 26 consecutive nucleotides) having complementarity with the target domain, e.g., the targeting domain is 26 nucleotides in length; and there are at least 16, 19, 21, 26, 31, 32, 36,

41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain.

II. Methods for Designing gRNAs

Methods for designing gRNAs are described herein, including methods for selecting, designing and validating target domains. Exemplary targeting domains are also provided herein. Targeting Domains discussed herein can be incorporated into the gRNAs described herein.

Methods for selection and validation of target sequences as well as off-target analyses are described, e.g., in Mali et al., 2013 SCIENCE 339(6121): 823-826; Hsu et al. NAT

BIOTECHNOL, 31(9): 827-32; Fu et al., 2014 NAT BIOTECHNOL, doi: 10.1038/nbt.2808.

PubMed PMID: 24463574; Heigwer et al., 2014 NAT METHODS 11(2): 122-3. doi:

10.1038/nmeth.2812. PubMed PMID: 24481216; Bae et al., 2014 BIOTNFORMATICS PubMed PMID: 24463181; Xiao A et al., 2014 BIOINFORMATICS PubMed PMID: 24389662.

For example, a software tool can be used to optimize the choice of gRNA within a user's target sequence, e.g., to minimize total off-target activity across the genome. Off target activity may be other than cleavage. For each possible gRNA choice using S. pyogenes Cas9, the tool can identify all off-target sequences (preceding either NAG or NGG PAMs) across the genome that contain up to certain number (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10) of mismatched base-pairs. The cleavage efficiency at each off-target sequence can be predicted, e.g., using an experimentally-derived weighting scheme. Each possible gRNA is then ranked according to its total predicted off-target cleavage; the top-ranked gRNAs represent those that are likely to have the greatest on-target and the least off-target cleavage. Other functions, e.g., automated reagent design for CRISPR construction, primer design for the on-target Surveyor assay, and primer design for high-throughput detection and quantification of off- target cleavage via next-gen sequencing, can also be included in the tool. Candidate gRNA molecules can be evaluated by art-known methods or as described in Section IV herein.

The Targeting Domains discussed herein can be incorporated into the gRNAs described herein.

Strategies to identify gRNAs for S. pyogenes, S. aureus, and N. meningitidis to knock out the UL19 gene

As an example, three strategies were utilized to identify gRNAs for use with S.

pyogenes, S. aureus and N. meningitidis Cas9 enzymes. In the first strategy, guide RNAs (gRNAs) for use with the S. pyogenes (Tables 1A- 1C) Cas9 were identified using the publically available web-based ZiFiT server (Fu et al., Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014 Jan 26. doi: 10.1038/nbt.2808. PubMed PMID: 24463574, for the original references see Sander et al., 2007, NAR 35:W599-605; Sander et al., 2010, NAR 38: W462-8). In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available Repeat-Masker program. RepeatMmasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence. Following identification, gRNAs for use with a S. pyogenes Cas9 were ranked into 3 tiers.

The gRNAs in tier 1 were selected based on their distance to the target site and their orthogonality in the genome (based on the ZiFiT identification of close matches in the human genome containing an NGG PAM). As an example, for all targets, both 17-mer and 20-mer gRNAs were designed. gRNAs were also selected both for single-gRNA nuclease cutting and for the dual gRNA nickase strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for which strategy is based on several considerations:

1. For the dual nickase strategy, gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, it will also often result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus just causing indel mutations at the site of one gRNA.

While it can be desirable to have gRNAs start with a 5 ' G, this requirement was relaxed for some gRNAs in tier 1 in order to identify guides in the correct orientation, within a reasonable distance to the target position (i.e., within the first 500 bp of the coding sequence) and with a high level of orthogonality against the human genome. In order to find a pair for the dual-nickase strategy it was necessary to either extend the distance from the target position or remove the requirement for the 5'G. Tier 2 gRNAs were selected based on loction within the first 500 bp of the coding sequence in the HSV gene. Tier 3 gRNAs were selected based on their location in the coding sequence, but downstream of the first 500 bp of the HSV gene. Note that tiers are non-inclusive (each gRNA is listed only once). In certain instances, no gRNA was identified based on the criteria of the particular tier.

As discussed above, gRNAs were identified for single-gRNA nuclease cleavage as well as for a dual-gRNA paired "nickase" strategy, as indicated.

gRNAs for use with the N. meningitidis (Tables 1F-1G) and S. aureus (Tables 1D- 1E) Cas9s were identified manually by scanning genomic DNA sequence for the presence of PAM sequences. These gRNAs were separated into two tiers for each species. The first tier includes gRNAs selected based on location in the first 500 bp of the coding sequence of the HSV gene. The second tier includes gRNAs selected based on location in the coding sequence, but downstream of the first 500 bp of the HSV gene.

In a second strategy, Guide RNAs (gRNAs) for use with S. pyogenes, S. aureus and N. meningitidis Cas9s were identified using a DNA sequence searching algorithm. Guide RNA design was carried out using a custom guide RNA design software based on the public tool cas-offinder (reference:Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases., Bioinformatics. 2014 Feb 17. Bae S, Park J, Kim JS. PMID: 24463181). Said custom guide RNA design software scores guides after calculating their genomewide off-target propensity. Typically matches ranging from perfect matches to 7 mismatches are considered for guides ranging in length from 17 to 24. Once the off-target sites are computationally determined, an aggregate score is calculated for each guide and summarized in a tabular output using a web-interface. In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available

RepeatMasker program. RepeatMasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence.

Following identification, gRNAs were ranked into tiers based on their distance to the target site, their orthogonality and presence of a 5' G (based on identification of close matches in the human genome containing a relavant PAM (e.g., in the case of S. pyogenes, a NGG PAM, in the case of 5". aureus, a NNGRRT or NNGRRV PAM, and in the case of N. meningitidis, a NNNNGATT or NNNNGCTT PAM). Orthogonality refers to the number of sequences in the human genome that contain a minimum number of mismatches to the target sequence. A "high level of orthogonality" or "good orthogonality" may, for example, refer to

20- mer gRNAs that have no identical sequences in the human genome besides the intended target, nor any sequences that contain one or two mismatches in the target sequence.

Targeting domains with good orthogonality are selected to minimize off-target DNA cleavage.

As an example, for S. pyogenes and N. meningitidis targets, 17-mer, or 20-mer gRNAs were designed. As another example, for S. aureus targets, 18-mer, 19-mer, 20-mer,

21- mer, 22-mer, 23-mer and 24-mer gRNAs were designed. Targeting domains, disclosed herein, may comprise the 17-mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 18 or more nucleotides may comprise the 17-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the 18-mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 19 or more nucleotides may comprise the 18-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the 19-mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 20 or more nucleotides may comprise the 19-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the 20-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 21 or more nucleotides may comprise the 20-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the 21-mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 22 or more nucleotides may comprise the 21-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the

22- mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 23 or more nucleotides may comprise the 22-mer gRNAs described in Tables 5A-5E, 6A-6G or

7A-7C. Targeting domains, disclosed herein, may comprises the 23-mer described in Tables 5A-5E, 6A-6G or 7A-7C,e.g., the targeting domains of 24 or more nucleotides may comprise the 23-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. Targeting domains, disclosed herein, may comprises the 24-mer described in Tables 5A-5E, 6A-6G or 7A-7C, e.g., the targeting domains of 25 or more nucleotides may comprise the 24-mer gRNAs described in Tables 5A-5E, 6A-6G or 7A-7C. gRNAs were identified for both single-gRNA nuclease cleavage and for a dual-gRNA paired "nickase" strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for the dual-gRNA paired "nickase" strategy is based on two considerations: 1. gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, cleaving with dual nickase pairs can also result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus causing indel mutations at the site of one gRNA.

The targeting domains discussed herein can be incorporated into the gRNAs described herein.

gRNAs were identified and ranked into 5 tiers for S. pyogenes (Tables 5A-5E), and

N. meningitidis (Tables 7A-7C); and 7 tiers for S. aureus (Tables 6A-6G). For S. pyogenes, and N. meningitidis, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality and (3) the presence of 5'G. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) a high level of orthogonality. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) the presence of 5'G. The targeting domain for tier 4 gRNA molecules were selected based on distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon). The targeting domain for tier 5 gRNA molecules were selected based on distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon). For S. aureus, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, (3) the presence of 5'G and (4) PAM is NNGRRT. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, and (3) PAM is

NNGRRT. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRT. The targeting domain for tier 4 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRV. The targeting domain for tier 5 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon), (2) the presence of 5'G and (3) PAM is NNGRRT. The targeting domain for tier 6 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRT. The targeting domain for tier 7 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRV. Note that tiers are non- inclusive (each gRNA is listed only once for the strategy). In certain instances, no gRNA was identified based on the criteria of the particular tier.

Strategies to identify gRNAs for S. pyogenes, S. Aureus, and N. meningitidis to knock out the UL30 gene

As an example, three strategies were utilized to identify gRNAs for use with S.

pyogenes, S. aureus and N. meningitidis Cas9 enzymes.

In the first strategy, guide RNAs (gRNAs) for use with the S. pyogenes (Tables 2A-

2C) Cas9 were identified using the publically available web-based ZiFiT server (Fu et al., Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014 Jan 26. doi: 10.1038/nbt.2808. PubMed PMID: 24463574, for the original references see Sander et al., 2007, NAR 35:W599-605; Sander et al., 2010, NAR 38: W462-8). In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available Repeat-Masker program. RepeatMmasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence. Following identification, gRNAs for use with a S. pyogenes Cas9 were ranked into 3 tiers.

The gRNAs in tier 1 were selected based on their distance to the target site and their orthogonality in the genome (based on the ZiFiT identification of close matches in the human genome containing an NGG PAM). As an example, for all targets, both 17-mer and 20-mer gRNAs were designed. gRNAs were also selected both for single-gRNA nuclease cutting and for the dual gRNA nickase strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for which strategy is based on several considerations:

1. For the dual nickase strategy, gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, it will also often result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus just causing indel mutations at the site of one gRNA.

While it can be desirable to have gRNAs start with a 5 ' G, this requirement was relaxed for some gRNAs in tier 1 in order to identify guides in the correct orientation, within a reasonable distance to the target position (i.e., within the first 500 bp of the coding sequence) and with a high level of orthogonality against the human genome. In order to find a pair for the dual-nickase strategy it was necessary to either extend the distance from the target position or remove the requirement for the 5'G. Tier 2 gRNAs were selected based on loction within the first 500 bp of the coding sequence in the HSV gene. Tier 3 gRNAs were selected based on their location in the coding sequence, but downstream of the first 500 bp of the HSV gene. Note that tiers are non-inclusive (each gRNA is listed only once). In certain instances, no gRNA was identified based on the criteria of the particular tier.

As discussed above, gRNAs were identified for single-gRNA nuclease cleavage as well as for a dual-gRNA paired "nickase" strategy, as indicated.

gRNAs for use with the N. meningitidis (Tables 2F) and S. aureus (Tables 2D-2E)

Cas9s were identified manually by scanning genomic DNA sequence for the presence of PAM sequences. These gRNAs were separated into two tiers for each species. The first tier includes gRNAs selected based on location in the first 500 bp of the coding sequence of the HSV gene. The second tier includes gRNAs selected based on location in the coding sequence, but downstream of the first 500 bp of the HSV gene.

In a second strategy, Guide RNAs (gRNAs) for use with S. pyogenes, S. aureus and N. meningitidis Cas9s were identified using a DNA sequence searching algorithm. Guide RNA design was carried out using a custom guide RNA design software based on the public tool cas-offinder (reference:Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases., Bioinformatics. 2014 Feb 17. Bae S, Park J, Kim JS. PMID: 24463181). Said custom guide RNA design software scores guides after calculating their genomewide off-target propensity. Typically matches ranging from perfect matches to 7 mismatches are considered for guides ranging in length from 17 to 24. Once the off-target sites are computationally determined, an aggregate score is calculated for each guide and summarized in a tabular output using a web-interface. In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available

RepeatMasker program. RepeatMasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence.

Following identification, gRNAs were ranked into tiers based on their distance to the target site, their orthogonality and presence of a 5' G (based on identification of close matches in the human genome containing a relavant PAM (e.g., in the case of S. pyogenes, a NGG PAM, in the case of 5". aureus, a NNGRRT or NNGRRV PAM, and in the case of N. meningitidis, a NNNNGATT or NNNNGCTT PAM). Orthogonality refers to the number of sequences in the human genome that contain a minimum number of mismatches to the target sequence. A "high level of orthogonality" or "good orthogonality" may, for example, refer to

20- mer gRNAs that have no identical sequences in the human genome besides the intended target, nor any sequences that contain one or two mismatches in the target sequence.

Targeting domains with good orthogonality are selected to minimize off-target DNA cleavage.

As an example, for S. pyogenes and N. meningitidis targets, 17-mer, or 20-mer gRNAs were designed. As another example, for S. aureus targets, 18-mer, 19-mer, 20-mer,

21- mer, 22-mer, 23-mer and 24-mer gRNAs were designed. Targeting domains, disclosed herein, may comprise the 17-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 18 or more nucleotides may comprise the 17-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 18-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 19 or more nucleotides may comprise the 18-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 19-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 20 or more nucleotides may comprise the 19-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 20-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 21 or more nucleotides may comprise the 20-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 21-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 22 or more nucleotides may comprise the 21-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 22-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 23 or more nucleotides may comprise the 22-mer gRNAs described in Tables

8A-8E, 9A-9G or 10A-10D. Targeting domains, disclosed herein, may comprises the 23-mer described in Tables 8A-8E, 9A-9G or 10A-10D,e.g., the targeting domains of 24 or more nucleotides may comprise the 23-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A- 10D. Targeting domains, disclosed herein, may comprises the 24-mer described in Tables 8A-8E, 9A-9G or 10A-10D, e.g., the targeting domains of 25 or more nucleotides may comprise the 24-mer gRNAs described in Tables 8A-8E, 9A-9G or 10A-10D. gRNAs were identified for both single-gRNA nuclease cleavage and for a dual-gRNA paired "nickase" strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for the dual-gRNA paired "nickase" strategy is based on two considerations:

1. gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, cleaving with dual nickase pairs can also result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus causing indel mutations at the site of one gRNA.

The targeting domains discussed herein can be incorporated into the gRNAs described herein.

gRNAs were identified and ranked into 5 tiers for S. pyogenes (Tables 8A-8E), and N. meningitidis (Tables 10A-10D); and 7 tiers for 5". aureus (Tables 9A-9G). For 5".

pyogenes, and N. meningitidis, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality and (3) the presence of 5'G. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) a high level of orthogonality. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) the presence of 5'G. The targeting domain for tier 4 gRNA molecules were selected based on distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon). The targeting domain for tier 5 gRNA molecules were selected based on distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon). For S. aureus, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, (3) the presence of 5'G and (4) PAM is NNGRRT. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, and (3) PAM is

NNGRRT. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRT. The targeting domain for tier 4 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRV. The targeting domain for tier 5 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon), (2) the presence of 5'G and (3) PAM is NNGRRT. The targeting domain for tier 6 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRT. The targeting domain for tier 7 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRV. Note that tiers are non- inclusive (each gRNA is listed only once for the strategy). In certain instances, no gRNA was identified based on the criteria of the particular tier. Strategies to identify gRNAs for S. pyogenes, S. aureus, and N. meningitidis to knock out the UL48 gene

As an example, three strategies were utilized to identify gRNAs for use with S.

pyogenes, S. aureus and N. meningitidis Cas9 enzymes.

In the first strategy, guide RNAs (gRNAs) for use with the S. pyogenes (Tables 3A-

3C) Cas9 were identified using the publically available web-based ZiFiT server (Fu et al., Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014 Jan 26. doi: 10.1038/nbt.2808. PubMed PMID: 24463574, for the original references see Sander et al., 2007, NAR 35:W599-605; Sander et al., 2010, NAR 38: W462-8). In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available Repeat-Masker program. RepeatMmasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence. Following identification, gRNAs for use with a S. pyogenes Cas9 were ranked into 3 tiers.

The gRNAs in tier 1 were selected based on their distance to the target site and their orthogonality in the genome (based on the ZiFiT identification of close matches in the human genome containing an NGG PAM). As an example, for all targets, both 17-mer and 20-mer gRNAs were designed. gRNAs were also selected both for single-gRNA nuclease cutting and for the dual gRNA nickase strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for which strategy is based on several considerations:

1. For the dual nickase strategy, gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, it will also often result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus just causing indel mutations at the site of one gRNA.

While it can be desirable to have gRNAs start with a 5 ' G, this requirement was relaxed for some gRNAs in tier 1 in order to identify guides in the correct orientation, within a reasonable distance to the target position (i.e., within the first 500 bp of the coding sequence) and with a high level of orthogonality against the human genome. In order to find a pair for the dual-nickase strategy it was necessary to either extend the distance from the target position or remove the requirement for the 5'G. Tier 2 gRNAs were selected based on loction within the first 500 bp of the coding sequence in the HSV gene. Tier 3 gRNAs were selected based on their location in the coding sequence, but downstream of the first 500 bp of the HSV gene. Note that tiers are non-inclusive (each gRNA is listed only once). In certain instances, no gRNA was identified based on the criteria of the particular tier.

As discussed above, gRNAs were identified for single-gRNA nuclease cleavage as well as for a dual-gRNA paired "nickase" strategy, as indicated.

gRNAs for use with the N. meningitidis (Tables 3F) and S. aureus (Tables 3D-3E) Cas9s were identified manually by scanning genomic DNA sequence for the presence of PAM sequences. These gRNAs were separated into two tiers for each species. The first tier includes gRNAs selected based on location in the first 500 bp of the coding sequence of the HSV gene. The second tier includes gRNAs selected based on location in the coding sequence, but downstream of the first 500 bp of the HSV gene.

In a second strategy, Guide RNAs (gRNAs) for use with S. pyogenes, S. aureus and N. meningitidis Cas9s were identified using a DNA sequence searching algorithm. Guide RNA design was carried out using a custom guide RNA design software based on the public tool cas-offinder (reference:Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases., Bioinformatics. 2014 Feb 17. Bae S, Park J, Kim JS. PMID: 24463181). Said custom guide RNA design software scores guides after calculating their genomewide off-target propensity. Typically matches ranging from perfect matches to 7 mismatches are considered for guides ranging in length from 17 to 24. Once the off-target sites are computationally determined, an aggregate score is calculated for each guide and summarized in a tabular output using a web-interface. In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available

RepeatMasker program. RepeatMasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence. Following identification, gRNAs were ranked into tiers based on their distance to the target site, their orthogonality and presence of a 5' G (based on identification of close matches in the human genome containing a relavant PAM (e.g., in the case of S. pyogenes, a NGG PAM, in the case of 5". aureus, a NNGRRT or NNGRRV PAM, and in the case of N. meningitidis, a NNNNGATT or NNNNGCTT PAM). Orthogonality refers to the number of sequences in the human genome that contain a minimum number of mismatches to the target sequence. A "high level of orthogonality" or "good orthogonality" may, for example, refer to

20- mer gRNAs that have no identical sequences in the human genome besides the intended target, nor any sequences that contain one or two mismatches in the target sequence.

Targeting domains with good orthogonality are selected to minimize off-target DNA cleavage.

As an example, for S. pyogenes and N. meningitidis targets, 17-mer, or 20-mer gRNAs were designed. As another example, for S. aureus targets, 18-mer, 19-mer, 20-mer,

21- mer, 22-mer, 23-mer and 24-mer gRNAs were designed. Targeting domains, disclosed herein, may comprise the 17-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 18 or more nucleotides may comprise the 17-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 18-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 19 or more nucleotides may comprise the 18-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 19-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 20 or more nucleotides may comprise the 19-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 20-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 21 or more nucleotides may comprise the 20-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 21-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 22 or more nucleotides may comprise the 21-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 22-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 23 or more nucleotides may comprise the 22-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 23-mer described in Tables 11A-11E, 12A-12G or 13A-13B,e.g., the targeting domains of 24 or more nucleotides may comprise the 23-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. Targeting domains, disclosed herein, may comprises the 24-mer described in Tables 11A-11E, 12A-12G or 13A-13B, e.g., the targeting domains of 25 or more nucleotides may comprise the 24-mer gRNAs described in Tables 11A-11E, 12A-12G or 13A-13B. gRNAs were identified for both single-gRNA nuclease cleavage and for a dual-gRNA paired "nickase" strategy. Criteria for selecting gRNAs and the

determination for which gRNAs can be used for the dual-gRNA paired "nickase" strategy is based on two considerations:

1. gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, cleaving with dual nickase pairs can also result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus causing indel mutations at the site of one gRNA.

The targeting domains discussed herein can be incorporated into the gRNAs described herein. gRNAs were identified and ranked into 5 tiers for S. pyogenes (Tables 11A-11E), and N. meningitidis (Tables 13A-13B); and 7 tiers for S. aureus (Tables 12A-12G). For S.

pyogenes, and N. meningitidis, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality and (3) the presence of 5'G. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) a high level of orthogonality. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) the presence of 5'G. The targeting domain for tier 4 gRNA molecules were selected based on distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon). The targeting domain for tier 5 gRNA molecules were selected based on distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon). For S. aureus, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, (3) the presence of 5'G and (4) PAM is NNGRRT. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, and (3) PAM is NNGRRT. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRT. The targeting domain for tier 4 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRV. The targeting domain for tier 5 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon), (2) the presence of 5'G and (3) PAM is NNGRRT. The targeting domain for tier 6 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRT. The targeting domain for tier 7 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRV. Note that tiers are non- inclusive (each gRNA is listed only once for the strategy). In certain instances, no gRNA was identified based on the criteria of the particular tier.

Strategies to identify gRNAs for S. pyogenes, S. aureus, and N. meningitidis to knock out the UL54 gene

As an example, three strategies were utilized to identify gRNAs for use with S. pyogenes, S. aureus and N. meningitidis Cas9 enzymes.

In the first strategy, guide RNAs (gRNAs) for use with the S. pyogenes (Tables 4A- 4C) Cas9 were identified using the publically available web-based ZiFiT server (Fu et al., Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014 Jan 26. doi: 10.1038/nbt.2808. PubMed PMID: 24463574, for the original references see Sander et al., 2007, NAR 35:W599-605; Sander et al., 2010, NAR 38: W462-8). In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available Repeat-Masker program. RepeatMmasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence. Following identification, gRNAs for use with a S. pyogenes Cas9 were ranked into 3 tiers.

The gRNAs in tier 1 were selected based on their distance to the target site and their orthogonality in the genome (based on the ZiFiT identification of close matches in the human genome containing an NGG PAM). As an example, for all targets, both 17-mer and 20-mer gRNAs were designed. gRNAs were also selected both for single-gRNA nuclease cutting and for the dual gRNA nickase strategy. Criteria for selecting gRNAs and the determination for which gRNAs can be used for which strategy is based on several considerations:

1. For the dual nickase strategy, gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, it will also often result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus just causing indel mutations at the site of one gRNA.

While it can be desirable to have gRNAs start with a 5 ' G, this requirement was relaxed for some gRNAs in tier 1 in order to identify guides in the correct orientation, within a reasonable distance to the target position (i.e., within the first 500 bp of the coding sequence) and with a high level of orthogonality against the human genome. In order to find a pair for the dual-nickase strategy it was necessary to either extend the distance from the target position or remove the requirement for the 5'G. Tier 2 gRNAs were selected based on loction within the first 500 bp of the coding sequence in the HSV gene. Tier 3 gRNAs were selected based on their location in the coding sequence, but downstream of the first 500 bp of the HSV gene. Note that tiers are non-inclusive (each gRNA is listed only once). In certain instances, no gRNA was identified based on the criteria of the particular tier.

As discussed above, gRNAs were identified for single-gRNA nuclease cleavage as well as for a dual-gRNA paired "nickase" strategy, as indicated.

gRNAs for use with the N. meningitidis (Tables 4F-4G) and S. aureus (Tables 4D- 4E) Cas9s were identified manually by scanning genomic DNA sequence for the presence of PAM sequences. These gRNAs were separated into two tiers for each species. The first tier includes gRNAs selected based on location in the first 500 bp of the coding sequence of the HSV gene. The second tier includes gRNAs selected based on location in the coding sequence, but downstream of the first 500 bp of the HSV gene.

In a second strategy, Guide RNAs (gRNAs) for use with S. pyogenes, S. aureus and

N. meningitidis Cas9s were identified using a DNA sequence searching algorithm. Guide RNA design was carried out using a custom guide RNA design software based on the public tool cas-offinder (reference:Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases., Bioinformatics. 2014 Feb 17. Bae S, Park J, Kim JS. PMID: 24463181). Said custom guide RNA design software scores guides after calculating their genomewide off-target propensity. Typically matches ranging from perfect matches to 7 mismatches are considered for guides ranging in length from 17 to 24. Once the off-target sites are computationally determined, an aggregate score is calculated for each guide and summarized in a tabular output using a web-interface. In addition to identifying potential gRNA sites adjacent to PAM sequences, the software also identifies all PAM adjacent sequences that differ by 1, 2, 3 or more nucleotides from the selected gRNA sites. Genomic DNA sequence for each gene was obtained from the UCSC Genome browser and sequences were screened for repeat elements using the publically available

RepeatMasker program. RepeatMasker searches input DNA sequences for repeated elements and regions of low complexity. The output is a detailed annotation of the repeats present in a given query sequence.

Following identification, gRNAs were ranked into tiers based on their distance to the target site, their orthogonality and presence of a 5' G (based on identification of close matches in the human genome containing a relavant PAM (e.g., in the case of S. pyogenes, a NGG PAM, in the case of 5". aureus, a NNGRRT or NNGRRV PAM, and in the case of N. meningitidis, a NNNNGATT or NNNNGCTT PAM). Orthogonality refers to the number of sequences in the human genome that contain a minimum number of mismatches to the target sequence. A "high level of orthogonality" or "good orthogonality" may, for example, refer to 20-mer gRNAs that have no identical sequences in the human genome besides the intended target, nor any sequences that contain one or two mismatches in the target sequence.

Targeting domains with good orthogonality are selected to minimize off-target DNA cleavage.

As an example, for S. pyogenes and N. meningitidis targets, 17-mer, or 20-mer gRNAs were designed. As another example, for S. aureus targets, 18-mer, 19-mer, 20-mer, 21-mer, 22-mer, 23-mer and 24-mer gRNAs were designed. Targeting domains, disclosed herein, may comprise the 17-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 18 or more nucleotides may comprise the 17-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 18-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 19 or more nucleotides may comprise the 18-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 19-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 20 or more nucleotides may comprise the 19-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 20-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 21 or more nucleotides may comprise the 20-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 21-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 22 or more nucleotides may comprise the 21-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 22-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 23 or more nucleotides may comprise the 22-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 23-mer described in Tables 14A-14E, 15A-15G or 16A-16C,e.g., the targeting domains of 24 or more nucleotides may comprise the 23-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. Targeting domains, disclosed herein, may comprises the 24-mer described in Tables 14A-14E, 15A-15G or 16A-16C, e.g., the targeting domains of 25 or more nucleotides may comprise the 24-mer gRNAs described in Tables 14A-14E, 15A-15G or 16A-16C. gRNAs were identified for both single-gRNA nuclease cleavage and for a dual-gRNA paired "nickase" strategy. Criteria for selecting gRNAs and the

determination for which gRNAs can be used for the dual-gRNA paired "nickase" strategy is based on two considerations:

1. gRNA pairs should be oriented on the DNA such that PAMs are facing out and cutting with the D10A Cas9 nickase will result in 5' overhangs.

2. An assumption that cleaving with dual nickase pairs will result in deletion of the entire intervening sequence at a reasonable frequency. However, cleaving with dual nickase pairs can also result in indel mutations at the site of only one of the gRNAs. Candidate pair members can be tested for how efficiently they remove the entire sequence versus causing indel mutations at the site of one gRNA.

The targeting domains discussed herein can be incorporated into the gRNAs described herein.

gRNAs were identified and ranked into 5 tiers for S. pyogenes (Tables 14A-14E), and

N. meningitidis (Tables 16A-16C); and 7 tiers for S. aureus (Tables 15A-15G). For S.

pyogenes, and N. meningitidis, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality and (3) the presence of 5'G. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) a high level of orthogonality. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) the presence of 5'G. The targeting domain for tier 4 gRNA molecules were selected based on distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon). The targeting domain for tier 5 gRNA molecules were selected based on distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon). For S. aureus, the targeting domain for tier 1 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, (3) the presence of 5'G and (4) PAM is NNGRRT. The targeting domain for tier 2 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon), (2) a high level of orthogonality, and (3) PAM is

NNGRRT. The targeting domain for tier 3 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRT. The targeting domain for tier 4 gRNA molecules were selected based on (1) distance to a target site (e.g., start codon), e.g., within 500bp (e.g., downstream) of the target site (e.g., start codon) and (2) PAM is NNGRRV. The targeting domain for tier 5 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon), (2) the presence of 5'G and (3) PAM is NNGRRT. The targeting domain for tier 6 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRT. The targeting domain for tier 7 gRNA molecules were selected based on (1) distance to the target site (e.g., start codon), e.g., within reminder of the coding sequence, e.g., downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon) and (2) PAM is NNGRRV. Note that tiers are non- inclusive (each gRNA is listed only once for the strategy). In certain instances, no gRNA was identified based on the criteria of the particular tier.

In an embodiment, two or more (e.g., three or four) gRNA molecules are used with one Cas9 molecule. In another embodiment, when two or more (e.g., three or four) gRNAs are used with two or more Cas9 molecules, at least one Cas9 molecule is from a different species than the other Cas9 molecule(s). For example, when two gRNA molecules are used with two Cas9 molecules, one Cas9 molecule can be from one species and the other Cas9 molecule can be from a different species. Both Cas9 species are used to generate a single or double-strand break, as desired.

Any of the targeting domains in the tables described herein can be used with a Cas9 nickase molecule to generate a single strand break.

Any of the targeting domains in the tables described herein can be used with a Cas9 nuclease molecule to generate a double strand break.

When two gRNAs designed for use to target two Cas9 molecules, one Cas9 can be one species, the second Cas9 can be from a different species. Both Cas9 species are used to generate a single or double-strand break, as desired.

It is contemplated herein that any upstream gRNA described herein may be paired with any downstream gRNA described herein. When an upstream gRNA designed for use with one species of Cas9 is paired with a downstream gRNA designed for use from a different species of Cas9, both Cas9 species are used to generate a single or double-strand break, as desired. Exemplary Targeting Domains

Table 1A provides exemplary targeting domains for knocking out the UL19 gene selected according to first tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL19 gene and orthogonality against the human genome. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 1A

HSV2-UL19-106 - CCUGAGUACGAUCGAGG 17 415

HSV2-UL19-107 - AGAGUUUGACGCCCUGC 17 416

HSV2-UL19-108 - GAGUUUGACGCCCUGCU 17 417

HSV2-UL19-110 - GUCGCUCGUGCGCUUUC 17 418

HSV2-UL19-111 - GUGCGCUUUCUGGAGCU 17 419

HSV2-UL19-113 - CG U G U G CACCAAG U U CC 17 420

HSV2-UL19-114 - CACCAAGUUCCCGGAGC 17 421

HSV2-UL19-116 - UGGCUUACAUGAACGAA 17 422

HSV2-UL19-119 - GCAUAAUUACAUGACGA 17 423

HSV2-UL19-120 - CGAAGGUCAUCGACCGC 17 424

HSV2-UL19-121 - GAAGGUCAUCGACCGCC 17 425

HSV2-UL19-136 + UAAUGCCCGUCCCGUCC 17 426

HSV2-UL19-150 + CGUCAUGUAAUUAUGCA 17 427

HSV2-UL19-152 + AUGCACGGGCUGCUCGA 17 428

HSV2-UL19-154 + GCACGGGCUGCUCGACG 17 429

HSV2-UL19-155 + GGCUGCUCGACGGGGUG 17 430

HSV2-UL19-160 + GCCGUCGCGGGCGAUGA 17 431

HSV2-UL19-164 + UUCAUGUAAGCCAGCUC 17 432

HSV2-UL19-165 + UCAUGUAAGCCAGCUCC 17 433

HSV2-UL19-169 + GAAAGCGCACGAGCGAC 17 434

HSV2-UL19-174 + CAAACUCUACGUCAUAC 17 435

HSV2-UL19-182 + CUCGAUCGUACUCAGGA 17 436

HSV2-UL19-183 + CGUACUCAGGAUGGAGC 17 437

HSV2-UL19-184 + ACUCAGGAUGGAGCCGG 17 438

HSV2-UL19-195 + GGUAACCCGGGGGGUCG 17 439

HSV2-UL19-197 + CCCGGGGGGUCGCGGGC 17 440

Table IB provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp. Table IB

HSV2-UL19-59 + GACGGGGUGCGGGCCGUCGC 20 482

HSV2-UL19-60 + GCGGGCCGUCGCGGGCGAUG 20 483

HSV2-UL19-62 + GGGCCGUCGCGGGCGAUGAG 20 484

HSV2-UL19-63 + CGUCGCGGGCGAUGAGGGGC 20 485

HSV2-UL19-64 + CGCGGGCGAUGAGGGGCUGG 20 486

HSV2-UL19-67 + GUAAGCCAGCUCCGGGAACU 20 487

HSV2-UL19-68 + CU UGGUGCACACGCACGCCA 20 488

HSV2-UL19-69 + UGCACACGCACGCCACGGAC 20 489

HSV2-UL19-71 + CAGAAAGCGCACGAGCGACA 20 490

HSV2-UL19-72 + GAGCGACAGGGUGUUGCAGU 20 491

HSV2-UL19-73 + UGUUGCAGUAGGACCCCAGC 20 492

HSV2-UL19-74 + GUUGCAGUAGGACCCCAGCA 20 493

HSV2-UL19-76 + GUCAUACAGGCUGUUUUCGU 20 494

HSV2-UL19-77 + GCUGUUUUCGUCGGAGCGCA 20 495

HSV2-UL19-78 + GUUUUCGUCGGAGCGCACGG 20 496

HSV2-UL19-79 + CGAAAAAAUCAAAGAGUCUG 20 497

HSV2-UL19-80 + AAAAAUCAAAGAGUCUGCGG 20 498

HSV2-UL19-81 + AAAAUCAAAGAGUCUGCGGU 20 499

HSV2-UL19-82 + ACGCCACCUCGAUCGUACUC 20 500

HSV2-UL19-86 + GUACUCAGGAUGGAGCCGGU 20 501

HSV2-UL19-87 + UCAGGAUGGAGCCGGUGGGC 20 502

HSV2-UL19-88 + GAUGGAGCCGGUGGGCAGGA 20 503

HSV2-UL19-89 + GCCGGUGGGCAGGAUGGCCG 20 504

HSV2-UL19-90 + GCAGGAUGGCCGCGGCGUAC 20 505

HSV2-UL19-91 + GCCGCGGCGUACCGGUAACC 20 506

HSV2-UL19-92 + CCGCGGCGUACCGGUAACCC 20 507

HSV2-UL19-93 + CGCGGCGUACCGGUAACCCG 20 508

HSV2-UL19-94 + GCGGCGUACCGGUAACCCGG 20 509

HSV2-UL19-95 + CGGCGUACCGGUAACCCGGG 20 510

HSV2-UL19-97 + CCGGUAACCCGGGGGGUCGC 20 511

HSV2-UL19-99 + CGGGGGGUCGCGGGCAGGAG 20 512

HSV2-UL19-100 - UCCUGCCCGCGACCCCC 17 513

HSV2-UL19-101 - CCUGCCCGCGACCCCCC 17 514

HSV2-UL19-102 - GCGACCCCCCGGGUUAC 17 515

HSV2-UL19-103 - GGGUUACCGGUACGCCG 17 516

HSV2-UL19-104 - GCGGCCAUCCUGCCCAC 17 517

HSV2-UL19-109 - AGUUUGACGCCCUGCUG 17 518

HSV2-UL19-112 - GGAGCUCGGCCUGUCCG 17 519

HSV2-UL19-115 - CUGGCUUACAUGAACGA 17 520

HSV2-UL19-117 - AGGGCGUGUGCAGUUCG 17 521

HSV2-UL19-118 - CCCCUCAUCGCCCGCGA 17 522

HSV2-UL19-122 - GAACGCCGCCU UCAGCC 17 523

HSV2-UL19-123 - CUUCAGCCUGGCCACCG 17 524

HSV2-UL19-124 - GGCCAUUGCCCUGCUCA 17 525

HSV2-UL19-125 - GCCAUUGCCCUGCUCAC 17 526

HSV2-UL19-126 - CCAU UGCCCUGCUCACG 17 527 HSV2-UL19-127 - CAUUGCCCUGCUCACGG 17 528

HSV2-UL19-128 - UGCCCUGCUCACGGGGG 17 529

HSV2-UL19-129 - GCUCACGGGGGAGGCCC 17 530

HSV2-UL19-130 - ACGGGGGAGGCCCUGGA 17 531

HSV2-UL19-131 - CGGGGGAGGCCCUGGAC 17 532

HSV2-UL19-132 - GGAGGCCCUGGACGGGA 17 533

HSV2-UL19-133 - GAGGCCCUGGACGGGAC 17 534

HSV2-UL19-134 + GCUGGAUGGCGCGCAGC 17 535

HSV2-UL19-135 + CGCGCAGCUGGCGAUGC 17 536

HSV2-UL19-137 + AAUGCCCGUCCCGUCCA 17 537

HSV2-UL19-138 + GGGCCUCCCCCGUGAGC 17 538

HSV2-UL19-139 + GGCCUCCCCCGUGAGCA 17 539

HSV2-UL19-140 + CCCCGUGAGCAGGGCAA 17 540

HSV2-UL19-141 + GAGCAGGGCAAUGGCCU 17 541

HSV2-UL19-142 + CAGGGCAAUGGCCUCGG 17 542

HSV2-UL19-143 + CAAUGGCCUCGGUGGCC 17 543

HSV2-UL19-144 + CUCGGUGGCCAGGCUGA 17 544

HSV2-UL19-145 + GGUGGCCAGGCUGAAGG 17 545

HSV2-UL19-146 + GGCUGAAGGCGGCGUUC 17 546

HSV2-UL19-147 + GCUGAAGGCGGCGUUCA 17 547

HSV2-UL19-148 + AGGCGGCGUUCAGGGCC 17 548

HSV2-UL19-149 + CGGCGUUCAGGGCCCGG 17 549

HSV2-UL19-151 + GUCAUGUAAUUAUGCAC 17 550

HSV2-UL19-153 + UGCACGGGCUGCUCGAC 17 551

HSV2-UL19-156 + GCUGCUCGACGGGGUGC 17 552

HSV2-UL19-157 + CGGGGUGCGGGCCGUCG 17 553

HSV2-UL19-158 + GGGGUGCGGGCCGUCGC 17 554

HSV2-UL19-159 + GGCCGUCGCGGGCGAUG 17 555

HSV2-UL19-161 + CCGUCGCGGGCGAUGAG 17 556

HSV2-UL19-162 + CGCGGGCGAUGAGGGGC 17 557

HSV2-UL19-163 + GGGCGAUGAGGGGCUGG 17 558

HSV2-UL19-166 + AGCCAGCUCCGGGAACU 17 559

HSV2-UL19-167 + GGUGCACACGCACGCCA 17 560

HSV2-UL19-168 + ACACGCACGCCACGGAC 17 561

HSV2-UL19-170 + AAAGCGCACGAGCGACA 17 562

HSV2-UL19-171 + CGACAGGGUGUUGCAGU 17 563

HSV2-UL19-172 + UGCAGUAGGACCCCAGC 17 564

HSV2-UL19-173 + GCAGUAGGACCCCAGCA 17 565

HSV2-UL19-175 + AUACAGGCUGUUUUCGU 17 566

HSV2-UL19-176 + GUUUUCGUCGGAGCGCA 17 567

HSV2-UL19-177 + UUCGUCGGAGCGCACGG 17 568

HSV2-UL19-178 + AAAAAU CAAAG AG UCU G 17 569

HSV2-UL19-179 + AAU CAAAG AG U CU GCG G 17 570

HSV2-UL19-180 + AUCAAAGAGUCUGCGGU 17 571

HSV2-UL19-181 + CCACCUCGAUCGUACUC 17 572

HSV2-UL19-185 + CUCAGGAUGGAGCCGGU 17 573 HSV2-UL19-186 + GGAUGGAGCCGGUGGGC 17 574

HSV2-UL19-187 + GGAGCCGGUGGGCAGGA 17 575

HSV2-UL19-188 + GGUGGGCAGGAUGGCCG 17 576

HSV2-UL19-189 + GGAUGGCCGCGGCGUAC 17 577

HSV2-UL19-190 + GCGGCGUACCGGUAACC 17 578

HSV2-UL19-191 + CGGCGUACCGGUAACCC 17 579

HSV2-UL19-192 + GGCGUACCGGUAACCCG 17 580

HSV2-UL19-193 + GCG U ACCGG UAACCCGG 17 581

HSV2-UL19-194 + CGUACCGGUAACCCGGG 17 582

HSV2-UL19-196 + GUAACCCGGGGGGUCGC 17 583

HSV2-UL19-198 + GGGGUCGCGGGCAGGAG 17 584

Table 1C provides exemplary targeting domains for knocking out the UL19 gene selected according to the third tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of the UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 1C

HSV2-UL19-356 - GCCUGGCGACCAGGGUUGCC 20 596

HSV2-UL19-357 - CCUGGCGACCAGGGUUGCCC 20 597

HSV2-UL19-358 - CAGGGUUGCCCGGGCGACCC 20 598

HSV2-UL19-359 - CCCUGGUCGCCGAGCUGAAG 20 599

HSV2-UL19-360 - UUGCGACACGAGCUUCUUCC 20 600

HSV2-UL19-361 - UGCGACACGAGCUUCUUCCU 20 601

HSV2-UL19-362 - CACGAGCUUCUUCCUGGGCA 20 602

HSV2-UL19-363 - GAGCUUCUUCCUGGGCAAGG 20 603

HSV2-UL19-364 - AGCUUCUUCCUGGGCAAGGC 20 604

HSV2-UL19-365 - CAAGGCGGGCCAUCGCCGCG 20 605

HSV2-UL19-366 - CCAUCGCCGCGAGGCCAUCG 20 606

HSV2-UL19-367 - GCCGCGAGGCCAUCGAGGCC 20 607

HSV2-UL19-368 - GGCCAUCGAGGCCUGGCUCG 20 608

HSV2-UL19-369 - CUGGCUCGUGGACCUGACCA 20 609

HSV2-UL19-370 - CACGGCGACGCAGCCGUCCG 20 610

HSV2-UL19-371 - CUGACGCACGCCGACACGCG 20 611

HSV2-UL19-372 - UGACGCACGCCGACACGCGC 20 612

HSV2-UL19-373 - CGCACGCCGACACGCGCGGG 20 613

HSV2-UL19-374 - CGCCGACACGCGCGGGCGGC 20 614

HSV2-UL19-375 - ACGCGCGGGCGGCCGGUCGA 20 615

HSV2-UL19-376 - CGCGCGGGCGGCCGGUCGAC 20 616

HSV2-UL19-377 - GCGCGGGCGGCCGGUCGACG 20 617

HSV2-UL19-378 - GCGGCCGGUCGACGGGGUGC 20 618

HSV2-UL19-379 - CCUCCUGCAGUCCUUCCUGA 20 619

HSV2-UL19-380 - CCUGCAGUCCUUCCUGAAGG 20 620

HSV2-UL19-381 - GCAGUCCUUCCUGAAGGUGG 20 621

HSV2-UL19-382 - CCUGAAGGUGGAGGACACCG 20 622

HSV2-UL19-383 - GGACACCGAGGCCGACGUGC 20 623

HSV2-UL19-384 - GCCGACGUGCCGGUGACCUA 20 624

HSV2-UL19-385 - GCCGGUGACCUACGGCGAGA 20 625

HSV2-UL19-386 - UACGGCGAGAUGGUCUUGAA 20 626

HSV2-UL19-387 - ACGGCGAGAUGGUCUUGAAC 20 627

HSV2-UL19-388 - CGGCGAGAUGGUCUUGAACG 20 628

HSV2-UL19-389 - GAACGGGGCCAACCUCGUCA 20 629

HSV2-UL19-390 - GGCCAACCUCGUCACGGCGC 20 630

HSV2-UL19-391 - CCUCGUCACGGCGCUGGUGA 20 631

HSV2-UL19-392 - CUCGUCACGGCGCUGGUGAU 20 632

HSV2-UL19-393 - CACGGCGCUGGUGAUGGGCA 20 633

HSV2-UL19-394 - UGGUGAUGGGCAAGGCCGUG 20 634

HSV2-UL19-395 - GGGCAAGGCCGUGCGGAGCC 20 635

HSV2-UL19-396 - CGUGCGGAGCCUGGACGACG 20 636

HSV2-UL19-397 - GUGCGGAGCCUGGACGACGU 20 637

HSV2-UL19-398 - CGACGUGGGCCGCCACCUGC 20 638

HSV2-UL19-399 - CCGCCACCUGCUGGAUAUGC 20 639

HSV2-UL19-400 - CCACCUGCUGGAUAUGCAGG 20 640

HSV2-UL19-401 - UAUGCAGGAGGAGCAACUCG 20 641 HSV2-UL19-402 - AGGAGCAACUCGAGGCGAAC 20 642

HSV2-UL19-403 - GGAGCAACUCGAGGCGAACC 20 643

HSV2-UL19-404 - CGAGGCGAACCGGGAGACGC 20 644

HSV2-UL19-405 - CCAGACAACGCGCGUGCGCG 20 645

HSV2-UL19-406 - AACGCGCGUGCGCGCGGAUC 20 646

HSV2-UL19-407 - GCGCGUGCGCGCGGAUCUGG 20 647

HSV2-UL19-408 - CGCGCGGAUCUGGUGGCCAU 20 648

HSV2-UL19-409 - AUCUGGUGGCCAUAGGCGAC 20 649

HSV2-UL19-410 - GGUGGCCAUAGGCGACAGGC 20 650

HSV2-UL19-411 - AGGCGACAGGCUGGUCUUCC 20 651

HSV2-UL19-412 - CGACAGGCUGGUCUUCCUGG 20 652

HSV2-UL19-413 - GCUGGUCUUCCUGGAGGCCC 20 653

HSV2-UL19-414 - CACCAACGUGCCCUACCCCC 20 654

HSV2-UL19-415 - CAACGUGCCCUACCCCCUGG 20 655

HSV2-UL19-416 - AACGUGCCCUACCCCCUGGU 20 656

HSV2-UL19-417 - CUACCCCCUGGUGGGCGCCA 20 657

HSV2-UL19-418 - CCUGACGUUCGUCCUGCCCC 20 658

HSV2-UL19-419 - CUGACGUUCGUCCUGCCCCU 20 659

HSV2-UL19-420 - UGACGUUCGUCCUGCCCCUG 20 660

HSV2-UL19-421 - GCCCCUGGGGCUGUUCAACC 20 661

HSV2-UL19-422 - GGGGCUGUUCAACCCGGCCA 20 662

HSV2-UL19-423 - GAGCGCUUCGCCGCGCACGC 20 663

HSV2-UL19-424 - AGCGCUUCGCCGCGCACGCC 20 664

HSV2-UL19-425 - GCGCU UCGCCGCGCACGCCG 20 665

HSV2-UL19-426 - CGCCGCGCACGCCGGGGACC 20 666

HSV2-UL19-427 - GGGGACCUGGUGCCCGCCCC 20 667

HSV2-UL19-428 - GGUGCCCGCCCCCGGCCACC 20 668

HSV2-UL19-429 - AGCCCCGCGCGUUCCCUCCC 20 669

HSV2-UL19-430 - CUCCCCGGCAGCUGUUUUUU 20 670

HSV2-UL19-431 - UCCCCGGCAGCUGUUUUUUU 20 671

HSV2-UL19-432 - CCCCGGCAGCUGUUUUUUUG 20 672

HSV2-UL19-433 - GCAGCUGUUUUUUUGGGGAA 20 673

HSV2-UL19-434 - UUUUUGGGGAAAGGACCACC 20 674

HSV2-UL19-435 - GAAAGGACCACCAGGUUCUG 20 675

HSV2-UL19-436 - CCAGGUUCUGCGGCUGUCCA 20 676

HSV2-UL19-437 - GCGGCUGUCCAUGGAGAACG 20 677

HSV2-UL19-438 - CUGUCCAUGGAGAACGCGGU 20 678

HSV2-UL19-439 - UGUCCAUGGAGAACGCGGUC 20 679

HSV2-UL19-440 - UUCGCUCAUGAACAUCGACG 20 680

HSV2-UL19-441 - AUGAACAUCGACGCGGCCGU 20 681

HSV2-UL19-442 - UGAACAUCGACGCGGCCGUC 20 682

HSV2-UL19-443 - GAACAUCGACGCGGCCGUCG 20 683

HSV2-UL19-444 - AACAUCGACGCGGCCGUCGG 20 684

HSV2-UL19-445 - CG U G AACCACG ACCCCG U CG 20 685

HSV2-UL19-446 - GUCGAGGCCGCGAAUCCGUA 20 686

HSV2-UL19-447 - UCGAGGCCGCGAAUCCGUAC 20 687 HSV2-UL19-448 - CGAGGCCGCGAAUCCGUACG 20 688

HSV2-UL19-449 - UCCGUACGGGGCGUACGUCG 20 689

HSV2-UL19-450 - CGGGGCGUACGUCGCGGCCC 20 690

HSV2-UL19-451 - GCGUACGUCGCGGCCCCGGC 20 691

HSV2-UL19-452 - GUCGCGGCCCCGGCCGGCCC 20 692

HSV2-UL19-453 - GGCCCCGGCCGGCCCCGGCG 20 693

HSV2-UL19-454 - AGCAGCGUUUUCUGAACGCC 20 694

HSV2-UL19-455 - AGCGUUUUCUGAACGCCUGG 20 695

HSV2-UL19-456 - UGGCGGCAGCGCCUCGCCCA 20 696

HSV2-UL19-457 - GGCAGCGCCUCGCCCACGGC 20 697

HSV2-UL19-458 - GCAGCGCCUCGCCCACGGCC 20 698

HSV2-UL19-459 - GCCUCGCCCACGGCCGGGUC 20 699

HSV2-UL19-460 - UCGCCCACGGCCGGGUCCGG 20 700

HSV2-UL19-461 - CGCCCACGGCCGGGUCCGGU 20 701

HSV2-UL19-462 - CGCCGAGUGCCAGAUGACCG 20 702

HSV2-UL19-463 - GCAGCCCGACAACGCCAACC 20 703

HSV2-UL19-464 - CGACAACGCCAACCUGGCUC 20 704

HSV2-UL19-465 - CCCCGCGUUCGACUUCUUCG 20 705

HSV2-UL19-466 - CCCGCGUUCGACUUCUUCGC 20 706

HSV2-UL19-467 - GUUCGACUUCUUCGCGGGCG 20 707

HSV2-UL19-468 - GUGGCCGACGUCGAGCUUCC 20 708

HSV2-UL19-469 - GCCGACGUCGAGCUUCCCGG 20 709

HSV2-UL19-470 - UCCCGGCGGCGAAGUCCCCC 20 710

HSV2-UL19-471 - GGCGGCGAAGUCCCCCCGGC 20 711

HSV2-UL19-472 - CGAAGUCCCCCCGGCCGGUC 20 712

HSV2-UL19-473 - GAAGUCCCCCCGGCCGGUCC 20 713

HSV2-UL19-474 - AAGUCCCCCCGGCCGGUCCG 20 714

HSV2-UL19-475 - AGUCCCCCCGGCCGGUCCGG 20 715

HSV2-UL19-476 - GGCCGGUCCGGGGGCGAUCC 20 716

HSV2-UL19-477 - CGGGGGCGAUCCAGGCCACC 20 717

HSV2-UL19-478 - GAUCCAGGCCACCUGGCGCG 20 718

HSV2-UL19-479 - GCCACCUGGCGCGUGGUCAA 20 719

HSV2-UL19-480 - GGUCAACGGCAACCUGCCCC 20 720

HSV2-UL19-481 - CCUGCCCCUGGCGCUGUGUC 20 721

HSV2-UL19-482 - GCCCCUGGCGCUGUGUCCGG 20 722

HSV2-UL19-483 - CGGUGGCGUUUCGUGACGCC 20 723

HSV2-UL19-484 - GGUGGCGUUUCGUGACGCCC 20 724

HSV2-UL19-485 - GUGGCGUUUCGUGACGCCCG 20 725

HSV2-UL19-486 - GUUUCGUGACGCCCGGGGCC 20 726

HSV2-UL19-487 - GACGCCCGGGGCCUGGAGCU 20 727

HSV2-UL19-488 - CGGGGCCUGGAGCUCGGCGU 20 728

HSV2-UL19-489 - CGGCGUUGGCCGCCACGCCA 20 729

HSV2-UL19-490 - UGGCCGCCACGCCAUGGCGC 20 730

HSV2-UL19-491 - GCUACCAUAGCCGCCGUCCG 20 731

HSV2-UL19-492 - CUACCAUAGCCGCCGUCCGC 20 732

HSV2-UL19-493 - UACCAUAGCCGCCGUCCGCG 20 733 HSV2-UL19-494 - CGCCGUCCGCGGGGCGUUCG 20 734

HSV2-UL19-495 - GUUCGAGGACCGCAGCUACC 20 735

HSV2-UL19-496 - CGAGGACCGCAGCUACCCGG 20 736

HSV2-UL19-497 - CUGCUGCAAGCCGCGAUUCA 20 737

HSV2-UL19-498 - CGAGCACGUGUUCUGCGCCC 20 738

HSV2-UL19-499 - ACGUGUUCUGCGCCCUGGCG 20 739

HSV2-UL19-500 - CUCAGUGCAUCACCAGCUAC 20 740

HSV2-UL19-501 - CUGGAACAACACGCGAUGCG 20 741

HSV2-UL19-502 - GUUCGUGAACGACUACUCGC 20 742

HSV2-UL19-503 - UCGUACAUCGUGACCUACCU 20 743

HSV2-UL19-504 - CGUACAUCGUGACCUACCUC 20 744

HSV2-UL19-505 - GUACAUCGUGACCUACCUCG 20 745

HSV2-UL19-506 - UACAUCGUGACCUACCUCGG 20 746

HSV2-UL19-507 - CCUCGGGGGCGACCUCCCCG 20 747

HSV2-UL19-508 - CGACCUCCCCGAGGAGUGCA 20 748

HSV2-UL19-509 - AGGAGUGCAUGGCCGUGUAU 20 749

HSV2-UL19-510 - GGAGUGCAUGGCCGUGUAUC 20 750

HSV2-UL19-511 - CAUGGCCGUGUAUCGGGACC 20 751

HSV2-UL19-512 - GGCCGUGUAUCGGGACCUGG 20 752

HSV2-UL19-513 - GGACCUGGUGGCCCACGUCG 20 753

HSV2-UL19-514 - GGUGGCCCACGUCGAGGCCC 20 754

HSV2-UL19-515 - CGUCGAGGCCCUGGCCCAGC 20 755

HSV2-UL19-516 - CGAGGCCCUGGCCCAGCUGG 20 756

HSV2-UL19-517 - GGUGGACGACUUUACCCUGC 20 757

HSV2-UL19-518 - GUGGACGACUUUACCCUGCC 20 758

HSV2-UL19-519 - CGACUUUACCCUGCCGGGCC 20 759

HSV2-UL19-520 - UACCCUGCCGGGCCCGGAGC 20 760

HSV2-UL19-521 - ACCCUGCCGGGCCCGGAGCU 20 761

HSV2-UL19-522 - CUGCCGGGCCCGGAGCUGGG 20 762

HSV2-UL19-523 - UGCCGGGCCCGGAGCUGGGC 20 763

HSV2-UL19-524 - GGGCCCGGAGCUGGGCGGGC 20 764

HSV2-UL19-525 - GGAGCUGGGCGGGCAGGCUC 20 765

HSV2-UL19-526 - GAAUCACCUGAUGCGCGACC 20 766

HSV2-UL19-527 - CGCUGCUGCCGCCCCUCGUG 20 767

HSV2-UL19-528 - GCUGCUGCCGCCCCUCGUGU 20 768

HSV2-UL19-529 - CCCCUCGUGUGGGACUGCGA 20 769

HSV2-UL19-530 - CGACGGCCU UAUGCGACACG 20 770

HSV2-UL19-531 - CCUUAUGCGACACGCGGCCC 20 771

HSV2-UL19-532 - UGGACCGCCACCGAGACUGC 20 772

HSV2-UL19-533 - CCGAGACUGCCGGAUUGACG 20 773

HSV2-UL19-534 - CGAGACUGCCGGAUUGACGC 20 774

HSV2-UL19-535 - GAGACUGCCGGAUUGACGCG 20 775

HSV2-UL19-536 - AGACUGCCGGAUUGACGCGG 20 776

HSV2-UL19-537 - GACUGCCGGAUUGACGCGGG 20 777

HSV2-UL19-538 - ACUGCCGGAUUGACGCGGGG 20 778

HSV2-UL19-539 - GGGGCACGAGCCCGUCUACG 20 779 HSV2-UL19-540 - GCACGAGCCCGUCUACGCGG 20 780

HSV2-UL19-541 - CUACGCGGCGGCGUGCAACG 20 781

HSV2-UL19-542 - GGCGGCGUGCAACGUGGCGA 20 782

HSV2-UL19-543 - GCCGACU UUAACCGCAACGA 20 783

HSV2-UL19-544 - ACU U UAACCGCAACGACGGC 20 784

HSV2-UL19-545 - CCGGCUGCUGCACAACACCC 20 785

HSV2-UL19-546 - GCACAACACCCAGGCCCGCG 20 786

HSV2-UL19-547 - CGGCCGACGCCGCCGACGAC 20 787

HSV2-UL19-548 - CCGCCGACGACCGGCCGCAC 20 788

HSV2-UL19-549 - CGACGACCGGCCGCACCGGC 20 789

HSV2-UL19-550 - GGCCGCACCGGCCGGCCGAC 20 790

HSV2-UL19-551 - CAAAAUCUACUAUUACGUGC 20 791

HSV2-UL19-552 - CUACUAUUACGUGCUGGUGC 20 792

HSV2-UL19-553 - UGCUGGUGCCGGCCUUCUCG 20 793

HSV2-UL19-554 - GCUGGUGCCGGCCUUCUCGC 20 794

HSV2-UL19-555 - CUGGUGCCGGCCUUCUCGCG 20 795

HSV2-UL19-556 - UGGUGCCGGCCUUCUCGCGG 20 796

HSV2-UL19-557 - GCGGGGGCGCUGCUGCACCG 20 797

HSV2-UL19-558 - CGGGGGCGCUGCUGCACCGC 20 798

HSV2-UL19-559 - GGGGGCGCUGCUGCACCGCG 20 799

HSV2-UL19-560 - GGGGCGCUGCUGCACCGCGG 20 800

HSV2-UL19-561 - GUACGCCACGCUGCAGAACA 20 801

HSV2-UL19-562 - CGCCACGCUGCAGAACAUGG 20 802

HSV2-UL19-563 - GCUGCAGAACAUGGUGGUCC 20 803

HSV2-UL19-564 - GUGGUCCCGGAGAUCGCCCC 20 804

HSV2-UL19-565 - CCCGGAGAUCGCCCCCGGUG 20 805

HSV2-UL19-566 - CCCGCUGCAUCCCGCCAAUC 20 806

HSV2-UL19-567 - GCUGCAUCCCGCCAAUCUGG 20 807

HSV2-UL19-568 - CGCCAAUCUGGUGGCCAACA 20 808

HSV2-UL19-569 - GUCAAGCGCAUGUUCCACAA 20 809

HSV2-UL19-570 - UCAAGCGCAUGUUCCACAAC 20 810

HSV2-UL19-571 - AACGGGCGCGUCGUCGUCGA 20 811

HSV2-UL19-572 - ACGGGCGCGUCGUCGUCGAC 20 812

HSV2-UL19-573 - GCCCGCCAUGCUCACGCUGC 20 813

HSV2-UL19-574 - CAUGCUCACGCUGCAGGUGC 20 814

HSV2-UL19-575 - GCAGGUGCUGGCGCACAACA 20 815

HSV2-UL19-576 - CAACAUGGCCGAGCGCACGA 20 816

HSV2-UL19-577 - GACGGCGCUGCUGUGCUCCG 20 817

HSV2-UL19-578 - GUGCUCCGCGGCGCCCGACG 20 818

HSV2-UL19-579 - UGCUCCGCGGCGCCCGACGC 20 819

HSV2-UL19-580 - GGGCGCCAACACCGCGUCGA 20 820

HSV2-UL19-581 - GCCAACAUGCGCAUCUUCGA 20 821

HSV2-UL19-582 - CCAACAUGCGCAUCUUCGAC 20 822

HSV2-UL19-583 - CAACAUGCGCAUCUUCGACG 20 823

HSV2-UL19-584 - UUCGACGGGGCGCUGCACGC 20 824

HSV2-UL19-585 - GCACGCCGGCGUGCUGCUCA 20 825 HSV2-UL19-586 - G CU CAU G G CCCCCCAG CACC 20 826

HSV2-UL19-587 - CUGGACCACACCAUCCAAAA 20 827

HSV2-UL19-588 - GCCCGUCCACGCGCUGUUUG 20 828

HSV2-UL19-589 - CCCGUCCACGCGCUGUUUGC 20 829

HSV2-UL19-590 - GUUUGCGGGCGCCGACCACG 20 830

HSV2-UL19-591 - CAACGCGCCCAACUUCCCCC 20 831

HSV2-UL19-592 - CCCCCCGGCCCUGCGCGACC 20 832

HSV2-UL19-593 - CCUGGCGCGCGACGUCCCCC 20 833

HSV2-UL19-594 - CGACGUCCCCCUGGUCCCCC 20 834

HSV2-UL19-595 - CCCCCUGGUCCCCCCGGCCC 20 835

HSV2-UL19-596 - CCCCUGGUCCCCCCGGCCCU 20 836

HSV2-UL19-597 - CCCUGGUCCCCCCGGCCCUG 20 837

HSV2-UL19-598 - CCUGGUCCCCCCGGCCCUGG 20 838

HSV2-UL19-599 - CU CCU CCAU CCGCCAG CCCG 20 839

HSV2-UL19-600 - GCAGCACGCCCGCGAGAGCG 20 840

HSV2-UL19-601 - GCACGCCCGCGAGAGCGCGG 20 841

HSV2-UL19-602 - CACGCCCGCGAGAGCGCGGC 20 842

HSV2-UL19-603 - ACGCCCGCGAGAGCGCGGCG 20 843

HSV2-UL19-604 - CGCCCGCGAGAGCGCGGCGG 20 844

HSV2-UL19-605 - CGCGCUGACCUACGCGCUCA 20 845

HSV2-UL19-606 - GCUGACCUACGCGCUCAUGG 20 846

HSV2-UL19-607 - CUGACCUACGCGCUCAUGGC 20 847

HSV2-UL19-608 - UGACCUACGCGCUCAUGGCG 20 848

HSV2-UL19-609 - GUACUUCAAGAUGAGCCCCG 20 849

HSV2-UL19-610 - CCUGUAUCACCAGCUCAAGA 20 850

HSV2-UL19-611 - CUGUAUCACCAGCUCAAGAC 20 851

HSV2-UL19-612 - CUCAAGACGGGCCUCCACCC 20 852

HSV2-UL19-613 - UCAAGACGGGCCUCCACCCC 20 853

HSV2-UL19-614 - ACGGGCCUCCACCCCGGGUU 20 854

HSV2-UL19-615 - CGGGCCUCCACCCCGGGUUC 20 855

HSV2-UL19-616 - GGUUCGGGUUCACCGUCGUG 20 856

HSV2-UL19-617 - CGGGUUCACCGUCGUGCGGC 20 857

HSV2-UL19-618 - GCUGUUUUCCGAGCGCGCGU 20 858

HSV2-UL19-619 - GUUUUCCGAGCGCGCGUCGG 20 859

HSV2-UL19-620 - CGCGUCGGAGGCGUACUUUC 20 860

HSV2-UL19-621 - GCGUCGGAGGCGUACUUUCU 20 861

HSV2-UL19-622 - GUACUUUCUGGGCCAGCUCC 20 862

HSV2-UL19-623 - CU UUCUGGGCCAGCUCCAGG 20 863

HSV2-UL19-624 - CCAG G U G G CCCG CCACG AAA 20 864

HSV2-UL19-625 - CAGGUGGCCCGCCACGAAAC 20 865

HSV2-UL19-626 - GUGGCCCGCCACGAAACGGG 20 866

HSV2-UL19-627 - UGGCCCGCCACGAAACGGGC 20 867

HSV2-UL19-628 - GGCCCGCCACGAAACGGGCG 20 868

HSV2-UL19-629 - GCCCGCCACGAAACGGGCGG 20 869

HSV2-UL19-630 - CCCGCCACGAAACGGGCGGG 20 870

HSV2-UL19-631 - CCGCCACGAAACGGGCGGGG 20 871 HSV2-UL19-632 - UUCACGCUCACCCAGCCGCG 20 872

HSV2-UL19-633 - CACCCAGCCGCGCGGAAACG 20 873

HSV2-UL19-634 - GCCGCGCGGAAACGUGGACC 20 874

HSV2-UL19-635 - CCGCGCGGAAACGUGGACCU 20 875

HSV2-UL19-636 - CGGAAACGUGGACCUGGGUG 20 876

HSV2-UL19-637 - GGAAACGUGGACCUGGGUGU 20 877

HSV2-UL19-638 - UGUGGGCUACACCGCCGUCG 20 878

HSV2-UL19-639 - CUACACCGCCGUCGCGGCCA 20 879

HSV2-UL19-640 - UACACCGCCGUCGCGGCCAC 20 880

HSV2-UL19-641 - CACCG U CCG CAACCCCG U U A 20 881

HSV2-UL19-642 - CCGCAACCCCGUUACGGACA 20 882

HSV2-UL19-643 - CGCAACCCCGUUACGGACAU 20 883

HSV2-UL19-644 - CUCCCCCAAAACUUUUACCU 20 884

HSV2-UL19-645 - CAAAACUUUUACCUCGGCCG 20 885

HSV2-UL19-646 - AAAACUUUUACCUCGGCCGC 20 886

HSV2-UL19-647 - AAACU U U UACCUCGGCCGCG 20 887

HSV2-UL19-648 - CCCCCCGCUGCUAGACAACG 20 888

HSV2-UL19-649 - CGCCGUGUACCUGCGCAACG 20 889

HSV2-UL19-650 - GUACCUGCGCAACGCGGUCG 20 890

HSV2-UL19-651 - CCUGCGCAACGCGGUCGUGG 20 891

HSV2-UL19-652 - CUGCGCAACGCGGUCGUGGC 20 892

HSV2-UL19-653 - ACGCGGUCGUGGCGGGAAAC 20 893

HSV2-UL19-654 - GGUCGUGGCGGGAAACCGGC 20 894

HSV2-UL19-655 - GUCGUGGCGGGAAACCGGCU 20 895

HSV2-UL19-656 - UCGUGGCGGGAAACCGGCUG 20 896

HSV2-UL19-657 - GGCGGGAAACCGGCUGGGGC 20 897

HSV2-UL19-658 - GGGGCCGGCCCAGCCCCUCC 20 898

HSV2-UL19-659 - G CCCAG CCCCU CCCGG U CU U 20 899

HSV2-UL19-660 - CCCGGUCU UUGGCUGCGCCC 20 900

HSV2-UL19-661 - UUGGCUGCGCCCAGGUGCCG 20 901

HSV2-UL19-662 - GCCCAGGUGCCGCGGCGCGC 20 902

HSV2-UL19-663 - GGUGCCGCGGCGCGCCGGCA 20 903

HSV2-UL19-664 - CGGCGCGCCGGCAUGGACCA 20 904

HSV2-UL19-665 - GGCGCGCCGGCAUGGACCAC 20 905

HSV2-UL19-666 - CGCCGGCAUGGACCACGGGC 20 906

HSV2-UL19-667 - UGAGUUCAUCGCCACCCCCG 20 907

HSV2-UL19-668 - CAUCGCCACCCCCGUGGCCA 20 908

HSV2-UL19-669 - CG G ACAU CAACU ACU U U CG C 20 909

HSV2-UL19-670 - UUCGCCGGCCCUGCAACCCG 20 910

HSV2-UL19-671 - UCGCCGGCCCUGCAACCCGC 20 911

HSV2-UL19-672 - CGCCGGCCCUGCAACCCGCG 20 912

HSV2-UL19-673 - CUGCAACCCGCGGGGACGCG 20 913

HSV2-UL19-674 - AACCCGCGGGGACGCGCGGC 20 914

HSV2-UL19-675 - CCGCGGGGACGCGCGGCCGG 20 915

HSV2-UL19-676 - CGCGGCCGGCGGCGUGUACG 20 916

HSV2-UL19-677 - GCGGCCGGCGGCGUGUACGC 20 917 HSV2-UL19-678 - CGGCCGGCGGCGUGUACGCG 20 918

HSV2-UL19-679 - GGCCGGCGGCGUGUACGCGG 20 919

HSV2-UL19-680 - CGGCGUGUACGCGGGGGACA 20 920

HSV2-UL19-681 - CGUGUACGCGGGGGACAAGG 20 921

HSV2-UL19-682 - GUGUACGCGGGGGACAAGGA 20 922

HSV2-UL19-683 - UGUACGCGGGGGACAAGGAG 20 923

HSV2-UL19-684 - GUACGCGGGGGACAAGGAGG 20 924

HSV2-UL19-685 - AUAGCCCUCAUGUACGACCA 20 925

HSV2-UL19-686 - CGACCACGGCCAGAGCGACC 20 926

HSV2-UL19-687 - ACGGCCAGAGCGACCCGGCG 20 927

HSV2-UL19-688 - CGACCCGGCGCGGCCCUUCG 20 928

HSV2-UL19-689 - GGCGCGGCCCUUCGCGGCCA 20 929

HSV2-UL19-690 - UCGCGGCCACGGCCAACCCG 20 930

HSV2-UL19-691 - CGCGGCCACGGCCAACCCGU 20 931

HSV2-UL19-692 - CCAACCCGUGGGCGUCGCAG 20 932

HSV2-UL19-693 - GCGUCGCAGCGGUUCUCGUA 20 933

HSV2-UL19-694 - CGUCGCAGCGGUUCUCGUAC 20 934

HSV2-UL19-695 - GUCGCAGCGGUUCUCGUACG 20 935

HSV2-UL19-696 - UACGGGGACCUGCUGUACAA 20 936

HSV2-UL19-697 - ACGGGGACCUGCUGUACAAC 20 937

HSV2-UL19-698 - CGGGGACCUGCUGUACAACG 20 938

HSV2-UL19-699 - AACGGGGCCUAUCACCUCAA 20 939

HSV2-UL19-700 - ACGGGGCCUAUCACCUCAAC 20 940

HSV2-UL19-701 - CGGGGCCUAUCACCUCAACG 20 941

HSV2-UL19-702 - CU GCU U CAAG U U CU U CACCG 20 942

HSV2-UL19-703 - CUUCACCGCGGCCGACAUCA 20 943

HSV2-UL19-704 - CACGGCCAAACAUCGCUGCC 20 944

HSV2-UL19-705 - CUGCCUGGAGCGUCUCAUCG 20 945

HSV2-UL19-706 - GGAGCGUCUCAUCGUGGAAA 20 946

HSV2-UL19-707 - GAGCGUCUCAUCGUGGAAAC 20 947

HSV2-UL19-708 - UCUCAUCGUGGAAACGGGAU 20 948

HSV2-UL19-709 - CAUCGUGGAAACGGGAUCGG 20 949

HSV2-UL19-710 - AACGGGAUCGGCGGUAUCCA 20 950

HSV2-UL19-711 - CGUGCAGUUUAAGCGCCCGC 20 951

HSV2-UL19-712 - GUGCAGUUUAAGCGCCCGCC 20 952

HSV2-UL19-713 - UGCAGUUUAAGCGCCCGCCG 20 953

HSV2-UL19-714 - GCCGGGGUGCCGCGAGCUCG 20 954

HSV2-UL19-715 - GAGCUCGUGGAAGACCCGUG 20 955

HSV2-UL19-716 - AGACCCGUGCGGCCUGUUUC 20 956

HSV2-UL19-717 - CUGCUACGCAGCGCCCGCGA 20 957

HSV2-UL19-718 - UGCUACGCAGCGCCCGCGAU 20 958

HSV2-UL19-719 - GCUACGCAGCGCCCGCGAUG 20 959

HSV2-UL19-720 - ACGCAGCGCCCGCGAUGGGG 20 960

HSV2-UL19-721 - CUACGACGCCUCCCCGCUAA 20 961

HSV2-UL19-722 - UACGACGCCUCCCCGCUAAA 20 962

HSV2-UL19-723 - CGUCCAGGCCGUCCUGG 17 963 HSV2-UL19-724 - CUGGGGGCGUUUGAGCG 17 964

HSV2-UL19-725 - GGCGUUUGAGCGCGGCA 17 965

HSV2-UL19-726 - GAUGCUGCACGUGCUGU 17 966

HSV2-UL19-727 - GCACGUGCUGUUGGAGA 17 967

HSV2-UL19-728 - GGAGAAGGCGCCUCCCC 17 968

HSV2-UL19-729 - CAACGAUAUCUCGACAA 17 969

HSV2-UL19-730 - AACGAUAUCUCGACAAC 17 970

HSV2-UL19-731 - UCUCGACAACGGGCGCC 17 971

HSV2-UL19-732 - ACGGGCGCCUGGCGACC 17 972

HSV2-UL19-733 - CGGGCGCCUGGCGACCA 17 973

HSV2-UL19-734 - UGGCGACCAGGGUUGCC 17 974

HSV2-UL19-735 - GGCGACCAGGGUUGCCC 17 975

HSV2-UL19-736 - GGUUGCCCGGGCGACCC 17 976

HSV2-UL19-737 - UGGUCGCCGAGCUGAAG 17 977

HSV2-UL19-738 - CGACACGAGCUUCUUCC 17 978

HSV2-UL19-739 - GACACGAGCUUCUUCCU 17 979

HSV2-UL19-740 - GAGCUUCUUCCUGGGCA 17 980

HSV2-UL19-741 - CUUCUUCCUGGGCAAGG 17 981

HSV2-UL19-742 - UUCUUCCUGGGCAAGGC 17 982

HSV2-UL19-743 - GGCGGGCCAUCGCCGCG 17 983

HSV2-UL19-744 - UCGCCGCGAGGCCAUCG 17 984

HSV2-UL19-745 - GCGAGGCCAUCGAGGCC 17 985

HSV2-UL19-746 - CAUCGAGGCCUGGCUCG 17 986

HSV2-UL19-747 - GCUCGUGGACCUGACCA 17 987

HSV2-UL19-748 - GGCGACGCAGCCGUCCG 17 988

HSV2-UL19-749 - ACGCACGCCGACACGCG 17 989

HSV2-UL19-750 - CGCACGCCGACACGCGC 17 990

HSV2-UL19-751 - ACGCCGACACGCGCGGG 17 991

HSV2-UL19-752 - CGACACGCGCGGGCGGC 17 992

HSV2-UL19-753 - CGCGGGCGGCCGGUCGA 17 993

HSV2-UL19-754 - GCGGGCGGCCGGUCGAC 17 994

HSV2-UL19-755 - CGGGCGGCCGGUCGACG 17 995

HSV2-UL19-756 - GCCGGUCGACGGGGUGC 17 996

HSV2-UL19-757 - CCUGCAGUCCUUCCUGA 17 997

HSV2-UL19-758 - GCAGUCCUUCCUGAAGG 17 998

HSV2-UL19-759 - GUCCUUCCUGAAGGUGG 17 999

HSV2-UL19-760 - GAAGGUGGAGGACACCG 17 1000

HSV2-UL19-761 - CACCGAGGCCGACGUGC 17 1001

HSV2-UL19-762 - GACGUGCCGGUGACCUA 17 1002

HSV2-UL19-763 - GGUGACCUACGGCGAGA 17 1003

HSV2-UL19-764 - GGCGAGAUGGUCUUGAA 17 1004

HSV2-UL19-765 - GCGAGAUGGUCUUGAAC 17 1005

HSV2-UL19-766 - CGAGAUGGUCUUGAACG 17 1006

HSV2-UL19-767 - CGGGGCCAACCUCGUCA 17 1007

HSV2-UL19-768 - CAACCU CG U CACGGCG C 17 1008

HSV2-UL19-769 - CGUCACGGCGCUGGUGA 17 1009 HSV2-UL19-770 - GUCACGGCGCUGGUGAU 17 1010

HSV2-UL19-771 - GGCGCUGGUGAUGGGCA 17 1011

HSV2-UL19-772 - UGAUGGGCAAGGCCGUG 17 1012

HSV2-UL19-773 - CAAGGCCGUGCGGAGCC 17 1013

HSV2-UL19-774 - GCGGAGCCUGGACGACG 17 1014

HSV2-UL19-775 - CGGAGCCUGGACGACGU 17 1015

HSV2-UL19-776 - CGUGGGCCGCCACCUGC 17 1016

HSV2-UL19-777 - CCACCUGCUGGAUAUGC 17 1017

HSV2-UL19-778 - CCUGCUGGAUAUGCAGG 17 1018

HSV2-UL19-779 - GCAGGAGGAGCAACUCG 17 1019

HSV2-UL19-780 - AGCAACUCGAGGCGAAC 17 1020

HSV2-UL19-781 - GCAACUCGAGGCGAACC 17 1021

HSV2-UL19-782 - GGCGAACCGGGAGACGC 17 1022

HSV2-UL19-783 - GACAACGCGCGUGCGCG 17 1023

HSV2-UL19-784 - GCGCGUGCGCGCGGAUC 17 1024

HSV2-UL19-785 - CGUGCGCGCGGAUCUGG 17 1025

HSV2-UL19-786 - GCGGAUCUGGUGGCCAU 17 1026

HSV2-UL19-787 - UGGUGGCCAUAGGCGAC 17 1027

HSV2-UL19-788 - GGCCAUAGGCGACAGGC 17 1028

HSV2-UL19-789 - CGACAGGCUGGUCUUCC 17 1029

HSV2-UL19-790 - CAGGCUGGUCUUCCUGG 17 1030

HSV2-UL19-791 - GGUCUUCCUGGAGGCCC 17 1031

HSV2-UL19-792 - CAACGUGCCCUACCCCC 17 1032

HSV2-UL19-793 - CGUGCCCUACCCCCUGG 17 1033

HSV2-UL19-794 - GUGCCCUACCCCCUGGU 17 1034

HSV2-UL19-795 - CCCCCUGGUGGGCGCCA 17 1035

HSV2-UL19-796 - GACGUUCGUCCUGCCCC 17 1036

HSV2-UL19-797 - ACGUUCGUCCUGCCCCU 17 1037

HSV2-UL19-798 - CGUUCGUCCUGCCCCUG 17 1038

HSV2-UL19-799 - CCUGGGGCUGUUCAACC 17 1039

HSV2-UL19-800 - GCUGUUCAACCCGGCCA 17 1040

HSV2-UL19-801 - CGCUUCGCCGCGCACGC 17 1041

HSV2-UL19-802 - GCUUCGCCGCGCACGCC 17 1042

HSV2-UL19-803 - CUUCGCCGCGCACGCCG 17 1043

HSV2-UL19-804 - CGCGCACGCCGGGGACC 17 1044

HSV2-UL19-805 - GACCUGGUGCCCGCCCC 17 1045

HSV2-UL19-806 - GCCCGCCCCCGGCCACC 17 1046

HSV2-UL19-807 - CCCGCGCGUUCCCUCCC 17 1047

HSV2-UL19-808 - CCCGGCAGCUGUUUUUU 17 1048

HSV2-UL19-809 - CCGGCAGCUGUUUUUUU 17 1049

HSV2-UL19-810 - CGGCAGCUGUUUUUUUG 17 1050

HSV2-UL19-811 - GCUGUUUUUUUGGGGAA 17 1051

HSV2-UL19-812 - UUGGGGAAAGGACCACC 17 1052

HSV2-UL19-813 - AG G ACCACCAG G U UCU G 17 1053

HSV2-UL19-814 - GGUUCUGCGGCUGUCCA 17 1054

HSV2-UL19-815 - GCUGUCCAUGGAGAACG 17 1055 HSV2-UL19-816 - UCCAUGGAGAACGCGGU 17 1056

HSV2-UL19-817 - CCAUGGAGAACGCGGUC 17 1057

HSV2-UL19-818 - GCUCAUGAACAUCGACG 17 1058

HSV2-UL19-819 - AACAUCGACGCGGCCGU 17 1059

HSV2-UL19-820 - ACAUCGACGCGGCCGUC 17 1060

HSV2-UL19-821 - CAUCGACGCGGCCGUCG 17 1061

HSV2-UL19-822 - AUCGACGCGGCCGUCGG 17 1062

HSV2-UL19-823 - G AACCACG ACCCCG U CG 17 1063

HSV2-UL19-824 - GAGGCCGCGAAUCCGUA 17 1064

HSV2-UL19-825 - AGGCCGCGAAUCCGUAC 17 1065

HSV2-UL19-826 - GGCCGCGAAUCCGUACG 17 1066

HSV2-UL19-827 - GUACGGGGCGUACGUCG 17 1067

HSV2-UL19-828 - GGCGUACGUCGCGGCCC 17 1068

HSV2-UL19-829 - UACGUCGCGGCCCCGGC 17 1069

HSV2-UL19-830 - GCGGCCCCGGCCGGCCC 17 1070

HSV2-UL19-831 - CCCGGCCGGCCCCGGCG 17 1071

HSV2-UL19-832 - AGCGUUUUCUGAACGCC 17 1072

HSV2-UL19-833 - GUUUUCUGAACGCCUGG 17 1073

HSV2-UL19-834 - CGGCAGCGCCUCGCCCA 17 1074

HSV2-UL19-835 - AGCGCCUCGCCCACGGC 17 1075

HSV2-UL19-836 - GCGCCUCGCCCACGGCC 17 1076

HSV2-UL19-837 - UCGCCCACGGCCGGGUC 17 1077

HSV2-UL19-838 - CCCACGGCCGGGUCCGG 17 1078

HSV2-UL19-839 - CCACGGCCGGGUCCGGU 17 1079

HSV2-UL19-840 - CGAGUGCCAGAUGACCG 17 1080

HSV2-UL19-841 - GCCCGACAACGCCAACC 17 1081

HSV2-UL19-842 - CAACGCCAACCUGGCUC 17 1082

HSV2-UL19-843 - CGCGUUCGACUUCUUCG 17 1083

HSV2-UL19-844 - GCGUUCGACUUCUUCGC 17 1084

HSV2-UL19-845 - CGACUUCUUCGCGGGCG 17 1085

HSV2-UL19-846 - GCCGACGUCGAGCUUCC 17 1086

HSV2-UL19-847 - GACGUCGAGCUUCCCGG 17 1087

HSV2-UL19-848 - CGGCGGCGAAGUCCCCC 17 1088

HSV2-UL19-849 - GGCGAAGUCCCCCCGGC 17 1089

HSV2-UL19-850 - AGUCCCCCCGGCCGGUC 17 1090

HSV2-UL19-851 - GUCCCCCCGGCCGGUCC 17 1091

HSV2-UL19-852 - UCCCCCCGGCCGGUCCG 17 1092

HSV2-UL19-853 - CCCCCCGGCCGGUCCGG 17 1093

HSV2-UL19-854 - CGGUCCGGGGGCGAUCC 17 1094

HSV2-UL19-855 - GGGCGAUCCAGGCCACC 17 1095

HSV2-UL19-856 - CCAGGCCACCUGGCGCG 17 1096

HSV2-UL19-857 - ACCUGGCGCGUGGUCAA 17 1097

HSV2-UL19-858 - CAACGGCAACCUGCCCC 17 1098

HSV2-UL19-859 - GCCCCUGGCGCUGUGUC 17 1099

HSV2-UL19-860 - CCUGGCGCUGUGUCCGG 17 1100

HSV2-UL19-861 - UGGCGUUUCGUGACGCC 17 1101 HSV2-UL19-862 - GGCGUUUCGUGACGCCC 17 1102

HSV2-UL19-863 - GCGUUUCGUGACGCCCG 17 1103

HSV2-UL19-864 - UCGUGACGCCCGGGGCC 17 1104

HSV2-UL19-865 - GCCCGGGGCCUGGAGCU 17 1105

HSV2-UL19-866 - GGCCUGGAGCUCGGCGU 17 1106

HSV2-UL19-867 - CGUUGGCCGCCACGCCA 17 1107

HSV2-UL19-868 - CCGCCACGCCAUGGCGC 17 1108

HSV2-UL19-869 - ACCAUAGCCGCCGUCCG 17 1109

HSV2-UL19-870 - CCAUAGCCGCCGUCCGC 17 1110

HSV2-UL19-871 - CAUAGCCGCCGUCCGCG 17 1111

HSV2-UL19-872 - CGUCCGCGGGGCGUUCG 17 1112

HSV2-UL19-873 - CGAGGACCGCAGCUACC 17 1113

HSV2-UL19-874 - GGACCGCAGCUACCCGG 17 1114

HSV2-UL19-875 - CUGCAAGCCGCGAUUCA 17 1115

HSV2-UL19-876 - GCACGUGUUCUGCGCCC 17 1116

HSV2-UL19-877 - UGUUCUGCGCCCUGGCG 17 1117

HSV2-UL19-878 - AGUGCAUCACCAGCUAC 17 1118

HSV2-UL19-879 - GAACAACACGCGAUGCG 17 1119

HSV2-UL19-880 - CGUGAACGACUACUCGC 17 1120

HSV2-UL19-881 - UACAUCGUGACCUACCU 17 1121

HSV2-UL19-882 - ACAUCGUGACCUACCUC 17 1122

HSV2-UL19-883 - CAUCGUGACCUACCUCG 17 1123

HSV2-UL19-884 - AUCGUGACCUACCUCGG 17 1124

HSV2-UL19-885 - CGGGGGCGACCUCCCCG 17 1125

HSV2-UL19-886 - CCUCCCCGAGGAGUGCA 17 1126

HSV2-UL19-887 - AGUGCAUGGCCGUGUAU 17 1127

HSV2-UL19-888 - GUGCAUGGCCGUGUAUC 17 1128

HSV2-UL19-889 - GGCCGUGUAUCGGGACC 17 1129

HSV2-UL19-890 - CGUGUAUCGGGACCUGG 17 1130

HSV2-UL19-891 - CCUGGUGGCCCACGUCG 17 1131

HSV2-UL19-892 - GGCCCACGUCGAGGCCC 17 1132

HSV2-UL19-893 - CGAGGCCCUGGCCCAGC 17 1133

HSV2-UL19-894 - GGCCCUGGCCCAGCUGG 17 1134

HSV2-UL19-895 - GGACGACUUUACCCUGC 17 1135

HSV2-UL19-896 - GACGACU U UACCCUGCC 17 1136

HSV2-UL19-897 - CUUUACCCUGCCGGGCC 17 1137

HSV2-UL19-898 - CCUGCCGGGCCCGGAGC 17 1138

HSV2-UL19-899 - CUGCCGGGCCCGGAGCU 17 1139

HSV2-UL19-900 - CCGGGCCCGGAGCUGGG 17 1140

HSV2-UL19-901 - CGGGCCCGGAGCUGGGC 17 1141

HSV2-UL19-902 - CCCGGAGCUGGGCGGGC 17 1142

HSV2-UL19-903 - GCUGGGCGGGCAGGCUC 17 1143

HSV2-UL19-904 - UCACCUGAUGCGCGACC 17 1144

HSV2-UL19-905 - UGCUGCCGCCCCUCGUG 17 1145

HSV2-UL19-906 - GCUGCCGCCCCUCGUGU 17 1146

HSV2-UL19-907 - CUCGUGUGGGACUGCGA 17 1147 HSV2-UL19-908 - CGGCCUUAUGCGACACG 17 1148

HSV2-UL19-909 - UAUGCGACACGCGGCCC 17 1149

HSV2-UL19-910 - ACCGCCACCGAGACUGC 17 1150

HSV2-UL19-911 - AGACUGCCGGAUUGACG 17 1151

HSV2-UL19-912 - GACUGCCGGAUUGACGC 17 1152

HSV2-UL19-913 - ACUGCCGGAUUGACGCG 17 1153

HSV2-UL19-914 - CUGCCGGAUUGACGCGG 17 1154

HSV2-UL19-915 - UGCCGGAUUGACGCGGG 17 1155

HSV2-UL19-916 - GCCGGAUUGACGCGGGG 17 1156

HSV2-UL19-917 - GCACGAGCCCGUCUACG 17 1157

HSV2-UL19-918 - CGAGCCCGUCUACGCGG 17 1158

HSV2-UL19-919 - CGCGGCGGCGUGCAACG 17 1159

HSV2-UL19-920 - GGCGUGCAACGUGGCGA 17 1160

HSV2-UL19-921 - GACU U UAACCGCAACGA 17 1161

HSV2-UL19-922 - UUAACCGCAACGACGGC 17 1162

HSV2-UL19-923 - GCUGCUGCACAACACCC 17 1163

HSV2-UL19-924 - CA ACACCCAG G CCCG CG 17 1164

HSV2-UL19-925 - CCGACGCCGCCGACGAC 17 1165

HSV2-UL19-926 - CCGACGACCGGCCGCAC 17 1166

HSV2-UL19-927 - CGACCGGCCGCACCGGC 17 1167

HSV2-UL19-928 - CGCACCGGCCGGCCGAC 17 1168

HSV2-UL19-929 - AAUCUACUAUUACGUGC 17 1169

HSV2-UL19-930 - CUAUUACGUGCUGGUGC 17 1170

HSV2-UL19-931 - UGGUGCCGGCCUUCUCG 17 1171

HSV2-UL19-932 - GGUGCCGGCCUUCUCGC 17 1172

HSV2-UL19-933 - GUGCCGGCCUUCUCGCG 17 1173

HSV2-UL19-934 - UGCCGGCCUUCUCGCGG 17 1174

HSV2-UL19-935 - GGGGCGCUGCUGCACCG 17 1175

HSV2-UL19-936 - GGGCGCUGCUGCACCGC 17 1176

HSV2-UL19-937 - GGCGCUGCUGCACCGCG 17 1177

HSV2-UL19-938 - GCGCUGCUGCACCGCGG 17 1178

HSV2-UL19-939 - CGCCACGCUGCAGAACA 17 1179

HSV2-UL19-940 - CACGCUGCAGAACAUGG 17 1180

HSV2-UL19-941 - GCAGAACAUGGUGGUCC 17 1181

HSV2-UL19-942 - GUCCCGGAGAUCGCCCC 17 1182

HSV2-UL19-943 - GGAGAUCGCCCCCGGUG 17 1183

HSV2-UL19-944 - GCUGCAUCCCGCCAAUC 17 1184

HSV2-UL19-945 - GCAUCCCGCCAAUCUGG 17 1185

HSV2-UL19-946 - CAAUCUGGUGGCCAACA 17 1186

HSV2-UL19-947 - AAGCGCAUGUU CCACAA 17 1187

HSV2-UL19-948 - AGCGCAUGUUCCACAAC 17 1188

HSV2-UL19-949 - GGGCGCGUCGUCGUCGA 17 1189

HSV2-UL19-950 - GGCGCGUCGUCGUCGAC 17 1190

HSV2-UL19-951 - CGCCAUGCUCACGCUGC 17 1191

HSV2-UL19-952 - GCUCACGCUGCAGGUGC 17 1192

HSV2-UL19-953 - GGUGCUGGCG CACAACA 17 1193 HSV2-UL19-954 - CAUGGCCGAGCGCACGA 17 1194

HSV2-UL19-955 - GGCGCUGCUGUGCUCCG 17 1195

HSV2-UL19-956 - CUCCGCGGCGCCCGACG 17 1196

HSV2-UL19-957 - UCCGCGGCGCCCGACGC 17 1197

HSV2-UL19-958 - CGCCAACACCGCGUCGA 17 1198

HSV2-UL19-959 - AACAUGCGCAUCUUCGA 17 1199

HSV2-UL19-960 - ACAUGCGCAUCUUCGAC 17 1200

HSV2-UL19-961 - CAUGCGCAUCUUCGACG 17 1201

HSV2-UL19-962 - GACGGGGCGCUGCACGC 17 1202

HSV2-UL19-963 - CGCCGGCGUGCUGCUCA 17 1203

HSV2-UL19-964 - CAU GG CCCCCCAGCACC 17 1204

HSV2-UL19-965 - G ACCACACCAU CCAAAA 17 1205

HSV2-UL19-966 - CGUCCACGCGCUGUUUG 17 1206

HSV2-UL19-967 - GUCCACGCGCUGUUUGC 17 1207

HSV2-UL19-968 - UGCGGGCGCCGACCACG 17 1208

HSV2-UL19-969 - CG CG CCCAACU U CCCCC 17 1209

HSV2-UL19-970 - CCCGGCCCUGCGCGACC 17 1210

HSV2-UL19-971 - GGCGCGCGACGUCCCCC 17 1211

HSV2-UL19-972 - CGUCCCCCUGGUCCCCC 17 1212

HSV2-UL19-973 - CCUGGUCCCCCCGGCCC 17 1213

HSV2-UL19-974 - CUGGUCCCCCCGGCCCU 17 1214

HSV2-UL19-975 - UGGUCCCCCCGGCCCUG 17 1215

HSV2-UL19-976 - GGUCCCCCCGGCCCUGG 17 1216

HSV2-UL19-977 - CUCCAUCCGCCAGCCCG 17 1217

HSV2-UL19-978 - GCACGCCCGCGAGAGCG 17 1218

HSV2-UL19-979 - CGCCCGCGAGAGCGCGG 17 1219

HSV2-UL19-980 - GCCCGCGAGAGCGCGGC 17 1220

HSV2-UL19-981 - CCCGCGAGAGCGCGGCG 17 1221

HSV2-UL19-982 - CCGCGAGAGCGCGGCGG 17 1222

HSV2-UL19-983 - GCUGACCUACGCGCUCA 17 1223

HSV2-UL19-984 - GACCUACGCGCUCAUGG 17 1224

HSV2-UL19-985 - ACCUACGCGCUCAUGGC 17 1225

HSV2-UL19-986 - CCUACGCGCUCAUGGCG 17 1226

HSV2-UL19-987 - CU UCAAG AUG AGCCCCG 17 1227

HSV2-UL19-988 - GUAUCACCAGCUCAAGA 17 1228

HSV2-UL19-989 - UAUCACCAGCUCAAGAC 17 1229

HSV2-UL19-990 - AAGACGGGCCUCCACCC 17 1230

HSV2-UL19-991 - AGACGGGCCUCCACCCC 17 1231

HSV2-UL19-992 - GGCCUCCACCCCGGGUU 17 1232

HSV2-UL19-993 - GCCUCCACCCCGGGUUC 17 1233

HSV2-UL19-994 - UCGGGUUCACCGUCGUG 17 1234

HSV2-UL19-995 - GUUCACCGUCGUGCGGC 17 1235

HSV2-UL19-996 - GUUUUCCGAGCGCGCGU 17 1236

HSV2-UL19-997 - UUCCGAGCGCGCGUCGG 17 1237

HSV2-UL19-998 - GUCGGAGGCGUACUUUC 17 1238

HSV2-UL19-999 - UCGGAGGCGUACUUUCU 17 1239 HSV2-UL19-1000 - CUUUCUGGGCCAGCUCC 17 1240

HSV2-UL19-1001 - UCUGGGCCAGCUCCAGG 17 1241

HSV2-UL19-1002 - G G U G G CCCG CCACG AAA 17 1242

HSV2-UL19-1003 - GUGGCCCGCCACGAAAC 17 1243

HSV2-UL19-1004 - GCCCGCCACGAAACGGG 17 1244

HSV2-UL19-1005 - CCCGCCACGAAACGGGC 17 1245

HSV2-UL19-1006 - CCGCCACGAAACGGGCG 17 1246

HSV2-UL19-1007 - CGCCACGAAACGGGCGG 17 1247

HSV2-UL19-1008 - G CCACG AAACG G G CG G G 17 1248

HSV2-UL19-1009 - CCACGAAACGGGCGGGG 17 1249

HSV2-UL19-1010 - ACGCUCACCCAGCCGCG 17 1250

HSV2-UL19-1011 - CCAGCCGCGCGG AAACG 17 1251

HSV2-UL19-1012 - GCGCGGAAACGUGGACC 17 1252

HSV2-UL19-1013 - CGCGGAAACGUGGACCU 17 1253

HSV2-UL19-1014 - AAACGUGGACCUGGGUG 17 1254

HSV2-UL19-1015 - AACGUGGACCUGGGUGU 17 1255

HSV2-UL19-1016 - GGGCUACACCGCCGUCG 17 1256

HSV2-UL19-1017 - CACCGCCGUCGCGGCCA 17 1257

HSV2-UL19-1018 - ACCGCCGUCGCGGCCAC 17 1258

HSV2-UL19-1019 - CG U CCG CAACCCCG U U A 17 1259

HSV2-UL19-1020 - CAACCCCG U U ACG G ACA 17 1260

HSV2-UL19-1021 - AACCCCG U U ACGGACAU 17 1261

HSV2-UL19-1022 - CCCCAAAACU U U U ACCU 17 1262

HSV2-UL19-1023 - AACUUUUACCUCGGCCG 17 1263

HSV2-UL19-1024 - ACUUUUACCUCGGCCGC 17 1264

HSV2-UL19-1025 - CUUUUACCUCGGCCGCG 17 1265

HSV2-UL19-1026 - CCCGCUGCUAGACAACG 17 1266

HSV2-UL19-1027 - CGUGUACCUGCGCAACG 17 1267

HSV2-UL19-1028 - CCUGCGCAACGCGGUCG 17 1268

HSV2-UL19-1029 - GCGCAACGCGGUCGUGG 17 1269

HSV2-UL19-1030 - CGCAACGCGGUCGUGGC 17 1270

HSV2-UL19-1031 - CGGUCGUGGCGGGAAAC 17 1271

HSV2-UL19-1032 - CGUGGCGGGAAACCGGC 17 1272

HSV2-UL19-1033 - GUGGCGGGAAACCGGCU 17 1273

HSV2-UL19-1034 - UGGCGGGAAACCGGCUG 17 1274

HSV2-UL19-1035 - GGGAAACCGGCUGGGGC 17 1275

HSV2-UL19-1036 - GCCGGCCCAGCCCCUCC 17 1276

HSV2-UL19-1037 - CAG CCCCU CCCGG U CU U 17 1277

HSV2-UL19-1038 - GGUCUUUGGCUGCGCCC 17 1278

HSV2-UL19-1039 - GCUGCGCCCAGGUGCCG 17 1279

HSV2-UL19-1040 - CAGGUGCCGCGGCGCGC 17 1280

HSV2-UL19-1041 - GCCGCGGCGCGCCGGCA 17 1281

HSV2-UL19-1042 - CGCGCCGGCAUGGACCA 17 1282

HSV2-UL19-1043 - GCGCCGGCAUGGACCAC 17 1283

HSV2-UL19-1044 - CGGCAUGGACCACGGGC 17 1284

HSV2-UL19-1045 - GUUCAUCGCCACCCCCG 17 1285 HSV2-UL19-1046 - CGCCACCCCCGUGGCCA 17 1286

HSV2-UL19-1047 - ACAUCAACUACUUUCGC 17 1287

HSV2-UL19-1048 - GCCGGCCCUGCAACCCG 17 1288

HSV2-UL19-1049 - CCGGCCCUGCAACCCGC 17 1289

HSV2-UL19-1050 - CGGCCCUGCAACCCGCG 17 1290

HSV2-UL19-1051 - CAACCCGCGGGGACGCG 17 1291

HSV2-UL19-1052 - CCGCGGGGACGCGCGGC 17 1292

HSV2-UL19-1053 - CGGGGACGCGCGGCCGG 17 1293

HSV2-UL19-1054 - GGCCGGCGGCGUGUACG 17 1294

HSV2-UL19-1055 - GCCGGCGGCGUGUACGC 17 1295

HSV2-UL19-1056 - CCGGCGGCGUGUACGCG 17 1296

HSV2-UL19-1057 - CGGCGGCGUGUACGCGG 17 1297

HSV2-UL19-1058 - CGUGUACGCGGGGGACA 17 1298

HSV2-UL19-1059 - GUACGCGGGGGACAAGG 17 1299

HSV2-UL19-1060 - UACGCGGGGGACAAGGA 17 1300

HSV2-UL19-1061 - ACGCGGGGGACAAGGAG 17 1301

HSV2-UL19-1062 - CGCGGGGGACAAGGAGG 17 1302

HSV2-UL19-1063 - GCCCUCAUGUACGACCA 17 1303

HSV2-UL19-1064 - CCACGGCCAGAGCGACC 17 1304

HSV2-UL19-1065 - GCCAGAGCGACCCGGCG 17 1305

HSV2-UL19-1066 - CCCGGCGCGGCCCUUCG 17 1306

HSV2-UL19-1067 - GCGGCCCUUCGCGGCCA 17 1307

HSV2-UL19-1068 - CGGCCACGGCCAACCCG 17 1308

HSV2-UL19-1069 - GGCCACG G CCAACCCG U 17 1309

HSV2-UL19-1070 - ACCCGUGGGCGUCGCAG 17 1310

HSV2-UL19-1071 - UCGCAGCGGUUCUCGUA 17 1311

HSV2-UL19-1072 - CGCAGCGGUUCUCGUAC 17 1312

HSV2-UL19-1073 - GCAGCGGUUCUCGUACG 17 1313

HSV2-UL19-1074 - GGGGACCUGCUGUACAA 17 1314

HSV2-UL19-1075 - GGGACCUGCUGUACAAC 17 1315

HSV2-UL19-1076 - GGACCUGCUGUACAACG 17 1316

HSV2-UL19-1077 - GGGGCCUAUCACCUCAA 17 1317

HSV2-UL19-1078 - GGGCCUAUCACCUCAAC 17 1318

HSV2-UL19-1079 - GGCCUAUCACCUCAACG 17 1319

HSV2-UL19-1080 - CU U CAAG U UCU U CACCG 17 1320

HSV2-UL19-1081 - CACCGCGGCCGACAUCA 17 1321

HSV2-UL19-1082 - GGCCAAACAUCGCUGCC 17 1322

HSV2-UL19-1083 - CCUGGAGCGUCUCAUCG 17 1323

HSV2-UL19-1084 - GCGUCUCAUCGUGGAAA 17 1324

HSV2-UL19-1085 - CGUCUCAUCGUGGAAAC 17 1325

HSV2-UL19-1086 - CAUCGUGGAAACGGGAU 17 1326

HSV2-UL19-1087 - CGUGGAAACGGGAUCGG 17 1327

HSV2-UL19-1088 - GGGAUCGGCGGUAUCCA 17 1328

HSV2-UL19-1089 - GCAGUUUAAGCGCCCGC 17 1329

HSV2-UL19-1090 - CAGUUUAAGCGCCCGCC 17 1330

HSV2-UL19-1091 - AGUUUAAGCGCCCGCCG 17 1331 HSV2-UL19-1092 - GGGGUGCCGCGAGCUCG 17 1332

HSV2-UL19-1093 - CUCGUGGAAGACCCGUG 17 1333

HSV2-UL19-1094 - CCCGUGCGGCCUGUUUC 17 1334

HSV2-UL19-1095 - CUACGCAGCGCCCGCGA 17 1335

HSV2-UL19-1096 - UACGCAGCGCCCGCGAU 17 1336

HSV2-UL19-1097 - ACGCAGCGCCCGCGAUG 17 1337

HSV2-UL19-1098 - CAGCGCCCGCGAUGGGG 17 1338

HSV2-UL19-1099 - CGACGCCUCCCCGCUAA 17 1339

HSV2-UL19-1100 - GACGCCUCCCCGCUAAA 17 1340

HSV2-UL19-1101 + UACAGAGACAGGCCCUUUAG 20 1341

HSV2-UL19-1102 + ACAGAGACAGGCCCUUUAGC 20 1342

HSV2-UL19-1103 + CAGAGACAGGCCCUUUAGCG 20 1343

HSV2-UL19-1104 + AGACAGGCCCUUUAGCGGGG 20 1344

HSV2-UL19-1105 + AGAUGAGAUACUGCGUAAAG 20 1345

HSV2-UL19-1106 + GAUGAGAUACUGCGUAAAGU 20 1346

HSV2-UL19-1107 + UAAAGUGGGUCUCUCGCGCG 20 1347

HSV2-UL19-1108 + AAAGUGGGUCUCUCGCGCGU 20 1348

HSV2-UL19-1109 + GCGCGUGGGCCUCCCCAUCG 20 1349

HSV2-UL19-1110 + CGCGUGGGCCUCCCCAUCGC 20 1350

HSV2-UL19-1111 + CAUCGCGGGCGCUGCGUAGC 20 1351

HSV2-UL19-1112 + AUCGCGGGCGCUGCGUAGCA 20 1352

HSV2-UL19-1113 + GCGGGCGCUGCGUAGCAGGG 20 1353

HSV2-UL19-1114 + CGGGCGCUGCGUAGCAGGGC 20 1354

HSV2-UL19-1115 + GGGCGCUGCGUAGCAGGGCG 20 1355

HSV2-UL19-1116 + GCGUAGCAGGGCGGGGUCGC 20 1356

HSV2-UL19-1117 + CAGGGCGGGGUCGCUGGCGC 20 1357

HSV2-UL19-1118 + GGGUCGCUGGCGCAGGUGAU 20 1358

HSV2-UL19-1119 + GGUCGCUGGCGCAGGUGAUC 20 1359

HSV2-UL19-1120 + GCUGGCGCAGGUGAUCGGGU 20 1360

HSV2-UL19-1121 + UCGGGUAGGCUUCCUGAAAC 20 1361

HSV2-UL19-1122 + GCUUCCUGAAACAGGCCGCA 20 1362

HSV2-UL19-1123 + CUUCCUGAAACAGGCCGCAC 20 1363

HSV2-UL19-1124 + ACGGGUCUUCCACGAGCUCG 20 1364

HSV2-UL19-1125 + UCCACGAGCUCGCGGCACCC 20 1365

HSV2-UL19-1126 + ACGAGCUCGCGGCACCCCGG 20 1366

HSV2-UL19-1127 + CGAGCUCGCGGCACCCCGGC 20 1367

HSV2-UL19-1128 + GCGCUUAAACUGCACGUCGC 20 1368

HSV2-UL19-1129 + AAACUGCACGUCGCUGGCAG 20 1369

HSV2-UL19-1130 + CUGCACGUCGCUGGCAGCGG 20 1370

HSV2-UL19-1131 + GUCGCUGGCAGCGGUGGCCG 20 1371

HSV2-UL19-1132 + UUUCCACGAUGAGACGCUCC 20 1372

HSV2-UL19-1133 + ACGCUCCAGGCAGCGAUGUU 20 1373

HSV2-UL19-1134 + GCGAUGUUUGGCCGUGAUGU 20 1374

HSV2-UL19-1135 + UUUGGCCGUGAUGUCGGCCG 20 1375

HSV2-UL19-1136 + CGCGGUGAAGAACUUGAAGC 20 1376

HSV2-UL19-1137 + GCGGUGAAGAACUUGAAGCA 20 1377 HSV2-UL19-1138 + CGGUGAAGAACU UGAAG CAG 20 1378

HSV2-UL19-1139 + AGAACUUGAAGCAGGGGCUG 20 1379

HSV2-UL19-1140 + CUUGAAGCAGGGGCUGAGGA 20 1380

HSV2-UL19-1141 + UUGAAGCAGGGGCUGAGGAC 20 1381

HSV2-UL19-1142 + GCAGGGGCUGAGGACGGGCG 20 1382

HSV2-UL19-1143 + GGACGGGCGAGGCCCCGUUG 20 1383

HSV2-UL19-1144 + CGAGGCCCCGUUGAGGUGAU 20 1384

HSV2-UL19-1145 + GAUAGGCCCCGUUGUACAGC 20 1385

HSV2-UL19-1146 + GAGAACCGCUGCGACGCCCA 20 1386

HSV2-UL19-1147 + AGAACCGCUGCGACGCCCAC 20 1387

HSV2-UL19-1148 + CCGCUGCGACGCCCACGGGU 20 1388

HSV2-UL19-1149 + CGACGCCCACGGGUUGGCCG 20 1389

HSV2-UL19-1150 + CGGGUUGGCCGUGGCCGCGA 20 1390

HSV2-UL19-1151 + GGGUUGGCCGUGGCCGCGAA 20 1391

HSV2-UL19-1152 + GUGGCCGCGAAGGGCCGCGC 20 1392

HSV2-UL19-1153 + UGGCCGCGAAGGGCCGCGCC 20 1393

HSV2-UL19-1154 + AGGGCCGCGCCGGGUCGCUC 20 1394

HSV2-UL19-1155 + GCGCCGGGUCGCUCUGGCCG 20 1395

HSV2-UL19-1156 + UCUGGCCGUGGUCGUACAUG 20 1396

HSV2-UL19-1157 + CUGGCCGUGGUCGUACAUGA 20 1397

HSV2-UL19-1158 + GUCCCCCGCGUACACGCCGC 20 1398

HSV2-UL19-1159 + CCGCCGGCCGCGCGUCCCCG 20 1399

HSV2-UL19-1160 + CGCCGGCCGCGCGUCCCCGC 20 1400

HSV2-UL19-1161 + CGCGCGUCCCCGCGGGUUGC 20 1401

HSV2-UL19-1162 + GCGCGUCCCCGCGGGUUGCA 20 1402

HSV2-UL19-1163 + GUCCCCGCGGGUUGCAGGGC 20 1403

HSV2-UL19-1164 + GCGAAAGUAGUUGAUGUCCG 20 1404

HSV2-UL19-1165 + GUAGUUGAUGUCCGUGGCCA 20 1405

HSV2-UL19-1166 + UAGUUGAUGUCCGUGGCCAC 20 1406

HSV2-UL19-1167 + AGUUGAUGUCCGUGGCCACG 20 1407

HSV2-UL19-1168 + GUUGAUGUCCGUGGCCACGG 20 1408

HSV2-UL19-1169 + GAUGUCCGUGGCCACGGGGG 20 1409

HSV2-UL19-1170 + GGUGGCGAUGAACUCACACA 20 1410

HSV2-UL19-1171 + CACACACGGCAUCCUGCCCG 20 1411

HSV2-UL19-1172 + AUCCUGCCCGUGGUCCAUGC 20 1412

HSV2-UL19-1173 + UGGUCCAUGCCGGCGCGCCG 20 1413

HSV2-UL19-1174 + UGCCGGCGCGCCGCGGCACC 20 1414

HSV2-UL19-1175 + GCCGGCGCGCCGCGGCACCU 20 1415

HSV2-UL19-1176 + ACCUGGGCGCAGCCAAAGAC 20 1416

HSV2-UL19-1177 + CCUGGGCGCAGCCAAAGACC 20 1417

HSV2-UL19-1178 + GGGCGCAGCCAAAGACCGGG 20 1418

HSV2-UL19-1179 + GGCGCAGCCAAAGACCGGGA 20 1419

HSV2-UL19-1180 + GCGCAGCCAAAGACCGGGAG 20 1420

HSV2-UL19-1181 + AGCCAAAGACCGGGAGGGGC 20 1421

HSV2-UL19-1182 + GCCAAAGACCGGGAGGGGCU 20 1422

HSV2-UL19-1183 + AAGACCGGGAGGGGCUGGGC 20 1423 HSV2-UL19-1184 + GGGGCUGGGCCGGCCCCAGC 20 1424

HSV2-UL19-1185 + CCGCCACGACCGCG U UGCGC 20 1425

HSV2-UL19-1186 + GACCGCGUUGCGCAGGUACA 20 1426

HSV2-UL19-1187 + CGCGUUGCGCAGGUACACGG 20 1427

HSV2-UL19-1188 + GCGGCCGCGUUGUCUAGCAG 20 1428

HSV2-UL19-1189 + CGGCCGCGUUGUCUAGCAGC 20 1429

HSV2-UL19-1190 + GGCCGCGUUGUCUAGCAGCG 20 1430

HSV2-UL19-1191 + GCCGCGUUGUCUAGCAGCGG 20 1431

HSV2-UL19-1192 + CCGCGUUGUCUAGCAGCGGG 20 1432

HSV2-UL19-1193 + CGCGU UGUCUAGCAGCGGGG 20 1433

HSV2-UL19-1194 + CUAGCAGCGGGGGGGCCCCG 20 1434

HSV2-UL19-1195 + GCGGGGGGGCCCCGCGGCCG 20 1435

HSV2-UL19-1196 + CGCGGCCGAGGUAAAAGUUU 20 1436

HSV2-UL19-1197 + GCGGCCGAGGUAAAAGUUUU 20 1437

HSV2-UL19-1198 + CGGCCGAGGUAAAAGUUUUG 20 1438

HSV2-UL19-1199 + GGCCGAGGUAAAAGUUUUGG 20 1439

HSV2-UL19-1200 + CGAGGUAAAAGUUUUGGGGG 20 1440

HSV2-UL19-1201 + GAGGUUGCCCAUGUCCGUAA 20 1441

HSV2-UL19-1202 + AGGUUGCCCAUGUCCGUAAC 20 1442

HSV2-UL19-1203 + GGUUGCCCAUGUCCGUAACG 20 1443

HSV2-UL19-1204 + CCAUGUCCGUAACGGGGUUG 20 1444

HSV2-UL19-1205 + GUCCGUAACGGGGUUGCGGA 20 1445

HSV2-UL19-1206 + GGGGUUGCGGACGGUGCCCG 20 1446

HSV2-UL19-1207 + GACGGUGCCCGUGGCCGCGA 20 1447

HSV2-UL19-1208 + GGUGCCCGUGGCCGCGACGG 20 1448

HSV2-UL19-1209 + CGGCGGUGUAGCCCACACCC 20 1449

HSV2-UL19-1210 + CCCAGGUCCACGUUUCCGCG 20 1450

HSV2-UL19-1211 + GGUCCACGUUUCCGCGCGGC 20 1451

HSV2-UL19-1212 + GUCCACGUUUCCGCGCGGCU 20 1452

HSV2-UL19-1213 + U G ACCCCCCCG CCCG U U U CG 20 1453

HSV2-UL19-1214 + CCCCCCCGCCCGUUUCGUGG 20 1454

HSV2-UL19-1215 + CCCCCCGCCCGUUUCGUGGC 20 1455

HSV2-UL19-1216 + CCGU UUCGUGGCGGGCCACC 20 1456

HSV2-UL19-1217 + CGUGGCGGGCCACCUGGAGC 20 1457

HSV2-UL19-1218 + GUACGCCUCCGACGCGCGCU 20 1458

HSV2-UL19-1219 + CU CG G AAAACAGCACG U U CU 20 1459

HSV2-UL19-1220 + GCACGUUCUCGGUCACGAAG 20 1460

HSV2-UL19-1221 + GAAGCGGUCCUGCCGCACGA 20 1461

HSV2-UL19-1222 + CACGACG G U G AACCCG AACC 20 1462

HSV2-UL19-1223 + ACGACGGUGAACCCGAACCC 20 1463

HSV2-UL19-1224 + CGACGGUGAACCCGAACCCG 20 1464

HSV2-UL19-1225 + CGGUGAACCCGAACCCGGGG 20 1465

HSV2-UL19-1226 + UGAACCCGAACCCGGGGUGG 20 1466

HSV2-UL19-1227 + GGUGGAGGCCCGUCUUGAGC 20 1467

HSV2-UL19-1228 + CCGUCU UGAGCUGGUGAUAC 20 1468

HSV2-UL19-1229 + CGUCUUGAGCUGGUGAUACA 20 1469 HSV2-UL19-1230 + GAGCUGGUGAUACAGGGCCA 20 1470

HSV2-UL19-1231 + AGCUGGUGAUACAGGGCCAC 20 1471

HSV2-UL19-1232 + GCUGGUGAUACAGGGCCACG 20 1472

HSV2-UL19-1233 + GUACCCCGCCAUGAGCGCGU 20 1473

HSV2-UL19-1234 + UCUCCCCCGCCGCGCUCUCG 20 1474

HSV2-UL19-1235 + CUCCCCCGCCGCGCUCUCGC 20 1475

HSV2-UL19-1236 + CUCGCGGGCGUGCUGCACCA 20 1476

HSV2-UL19-1237 + UCGCGGGCGUGCUGCACCAC 20 1477

HSV2-UL19-1238 + GGGCGUGCUGCACCACGGGC 20 1478

HSV2-UL19-1239 + CGUGCUGCACCACGGGCUGG 20 1479

HSV2-UL19-1240 + CUGCACCACGGGCUGGCGGA 20 1480

HSV2-UL19-1241 + CACCACGGGCUGGCGGAUGG 20 1481

HSV2-UL19-1242 + GCGGAUGGAGGAGAAGUAGU 20 1482

HSV2-UL19-1243 + AGGAGAAGUAGUUGGCCCCC 20 1483

HSV2-UL19-1244 + GGAGAAGUAGUUGGCCCCCA 20 1484

HSV2-UL19-1245 + AAGUAGUUGGCCCCCAGGGC 20 1485

HSV2-UL19-1246 + AGUAGUUGGCCCCCAGGGCC 20 1486

HSV2-UL19-1247 + GUAGUUGGCCCCCAGGGCCG 20 1487

HSV2-UL19-1248 + UAGUUGGCCCCCAGGGCCGG 20 1488

HSV2-UL19-1249 + AGUUGGCCCCCAGGGCCGGG 20 1489

HSV2-UL19-1250 + CCCCCAGGGCCGGGGGGACC 20 1490

HSV2-UL19-1251 + CCCCAGGGCCGGGGGGACCA 20 1491

HSV2-UL19-1252 + CCCAGGGCCGGGGGGACCAG 20 1492

HSV2-UL19-1253 + CCAGGGCCGGGGGGACCAGG 20 1493

HSV2-UL19-1254 + CCAGGGGGACGUCGCGCGCC 20 1494

HSV2-UL19-1255 + CGUCGCGCGCCAGGUCGCGC 20 1495

HSV2-UL19-1256 + GUCGCGCGCCAGGUCGCGCA 20 1496

HSV2-UL19-1257 + CGCGCCAGGUCGCGCAGGGC 20 1497

HSV2-UL19-1258 + GCGCCAGGUCGCGCAGGGCC 20 1498

HSV2-UL19-1259 + CGCCAGGUCGCGCAGGGCCG 20 1499

HSV2-UL19-1260 + GCCAGGUCGCGCAGGGCCGG 20 1500

HSV2-UL19-1261 + CCAGGUCGCGCAGGGCCGGG 20 1501

HSV2-UL19-1262 + GCGCAGGGCCGGGGGGAAGU 20 1502

HSV2-UL19-1263 + CGCAGGGCCGGGGGGAAGUU 20 1503

HSV2-UL19-1264 + CGGGGGGAAGUUGGGCGCGU 20 1504

HSV2-UL19-1265 + AGUUGGGCGCGUUGGCCACG 20 1505

HSV2-UL19-1266 + GGGCGCGUUGGCCACGUGGU 20 1506

HSV2-UL19-1267 + CG G CG CCCG CAAACAG CG CG 20 1507

HSV2-UL19-1268 + GCCCGCAAACAGCGCGUGGA 20 1508

HSV2-UL19-1269 + CCCG CAAACAG CGCGUGGAC 20 1509

HSV2-UL19-1270 + CAAACAG CGCGUGGACGGGC 20 1510

HSV2-UL19-1271 + CGUAGAAGUAUUCGCCAUUU 20 1511

HSV2-UL19-1272 + GAAGUAUUCGCCAUUUUGGA 20 1512

HSV2-UL19-1273 + AUUCGCCAUUUUGGAUGGUG 20 1513

HSV2-UL19-1274 + CAUUUUGGAUGGUGUGGUCC 20 1514

HSV2-UL19-1275 + GGAUGGUGUGGUCCAGGUGC 20 1515 HSV2-UL19-1276 + GAUGGUGUGGUCCAGGUGCU 20 1516

HSV2-UL19-1277 + AUGGUGUGGUCCAGGUGCUG 20 1517

HSV2-UL19-1278 + UGGUGUGGUCCAGGUGCUGG 20 1518

HSV2-UL19-1279 + GGUGUGGUCCAGGUGCUGGG 20 1519

HSV2-UL19-1280 + GGGGGCCAUGAGCAGCACGC 20 1520

HSV2-UL19-1281 + CCCGUCGAAGAUGCGCAUGU 20 1521

HSV2-UL19-1282 + GCGCAUGUUGGCCGUCGACG 20 1522

HSV2-UL19-1283 + GUUGGCCGUCGACGCGGUGU 20 1523

HSV2-UL19-1284 + CGCGGUGUUGGCGCCCGCGU 20 1524

HSV2-UL19-1285 + GCGGUGUUGGCGCCCGCGUC 20 1525

HSV2-UL19-1286 + GGCGCCCGCGUCGGGCGCCG 20 1526

HSV2-UL19-1287 + CAGCAGCGCCGUCGUGCGCU 20 1527

HSV2-UL19-1288 + CAGCACCUGCAGCGUGAGCA 20 1528

HSV2-UL19-1289 + CACCUGCAGCGUGAGCAUGG 20 1529

HSV2-UL19-1290 + ACCUGCAGCGUGAGCAUGGC 20 1530

HSV2-UL19-1291 + CGACGACGACGCGCCCGU UG 20 1531

HSV2-UL19-1292 + GAACAUGCGCUUGACCGUGU 20 1532

HSV2-UL19-1293 + GACCGUGUUGGCCACCAGAU 20 1533

HSV2-UL19-1294 + CGUGUUGGCCACCAGAUUGG 20 1534

HSV2-UL19-1295 + GUGUUGGCCACCAGAUUGGC 20 1535

HSV2-UL19-1296 + ACCAGAUUGGCGGGAUGCAG 20 1536

HSV2-UL19-1297 + CCAGAUUGGCGGGAUGCAGC 20 1537

HSV2-UL19-1298 + GAUUGGCGGGAUGCAGCGGG 20 1538

HSV2-UL19-1299 + AUUGGCGGGAUGCAGCGGGU 20 1539

HSV2-UL19-1300 + GGCGGGAUGCAGCGGGUGGG 20 1540

HSV2-UL19-1301 + GCGGGAUGCAGCGGGUGGGC 20 1541

HSV2-UL19-1302 + CGGGAUGCAGCGGGUGGGCG 20 1542

HSV2-UL19-1303 + AUGCAGCGGGUGGGCGGGGU 20 1543

HSV2-UL19-1304 + CGGGUGGGCGGGGUCGGUCA 20 1544

HSV2-UL19-1305 + GGGUGGGCGGGGUCGGUCAC 20 1545

HSV2-UL19-1306 + GGGUCGGUCACGGGAUCGCU 20 1546

HSV2-UL19-1307 + GGUCGGUCACGGGAUCGCUC 20 1547

HSV2-UL19-1308 + AUCGCUCGGGCACUCCUCAC 20 1548

HSV2-UL19-1309 + UCGCUCGGGCACUCCUCACC 20 1549

HSV2-UL19-1310 + CGCUCGGGCACUCCUCACCG 20 1550

HSV2-UL19-1311 + GCUCGGGCACUCCUCACCGG 20 1551

HSV2-UL19-1312 + UCCUCACCGGGGGCGAUCUC 20 1552

HSV2-UL19-1313 + CCUCACCGGGGGCGAUCUCC 20 1553

HSV2-UL19-1314 + G ACCACCAU G U U CU G CAGCG 20 1554

HSV2-UL19-1315 + UCUGCAGCGUGGCGUACACG 20 1555

HSV2-UL19-1316 + UGGCGUACACGCGGUCGAAG 20 1556

HSV2-UL19-1317 + GCGGUCGAAGCGGACCCCCG 20 1557

HSV2-UL19-1318 + GCAGCAGCGCCCCCGCGAGA 20 1558

HSV2-UL19-1319 + CAGCGCCCCCGCGAGAAGGC 20 1559

HSV2-UL19-1320 + GCACGUAAUAGUAGAUUUUG 20 1560

HSV2-UL19-1321 + CGUAAUAGUAGAUUUUGUGG 20 1561 HSV2-UL19-1322 + AUAGUAGAUUUUGUGGUGGA 20 1562

HSV2-UL19-1323 + UUUGUGGUGGACGGUCCAGU 20 1563

HSV2-UL19-1324 + UGGUGGACGGUCCAGUCGGC 20 1564

HSV2-UL19-1325 + GGACGGUCCAGUCGGCCGGC 20 1565

HSV2-UL19-1326 + GUCCAGUCGGCCGGCCGGUG 20 1566

HSV2-UL19-1327 + AGUCGGCCGGCCGGUGCGGC 20 1567

HSV2-UL19-1328 + CGGCCGGUGCGGCCGGUCGU 20 1568

HSV2-UL19-1329 + CCGGUGCGGCCGGUCGUCGG 20 1569

HSV2-UL19-1330 + CGGCCGGUCGUCGGCGGCGU 20 1570

HSV2-UL19-1331 + CGUCGGCGGCGUCGGCCGCG 20 1571

HSV2-UL19-1332 + GUCGGCGGCGUCGGCCGCGC 20 1572

HSV2-UL19-1333 + CGGCGUCGGCCGCGCGGGCC 20 1573

HSV2-UL19-1334 + GGCGUCGGCCGCGCGGGCCU 20 1574

HSV2-UL19-1335 + CCUGGGUGUUGUGCAGCAGC 20 1575

HSV2-UL19-1336 + GCAGCAGCCGGCCGUCGUUG 20 1576

HSV2-UL19-1337 + GCCGUCGUUGCGGUUAAAGU 20 1577

HSV2-UL19-1338 + GUUGCACGCCGCCGCGUAGA 20 1578

HSV2-UL19-1339 + UUGCACGCCGCCGCGUAGAC 20 1579

HSV2-UL19-1340 + CGUGCCCCCCCGCGUCAAUC 20 1580

HSV2-UL19-1341 + CCGCGUCAAUCCGGCAGUCU 20 1581

HSV2-UL19-1342 + CGUCAAUCCGGCAGUCUCGG 20 1582

HSV2-UL19-1343 + CAAUCCGGCAGUCUCGGUGG 20 1583

HSV2-UL19-1344 + GGCAGUCUCGGUGGCGGUCC 20 1584

HSV2-UL19-1345 + GCAGUCUCGGUGGCGGUCCA 20 1585

HSV2-UL19-1346 + CCAGGGCCGCGUGUCGCAUA 20 1586

HSV2-UL19-1347 + GGCCGUCGCAGUCCCACACG 20 1587

HSV2-UL19-1348 + GCCGUCGCAGUCCCACACGA 20 1588

HSV2-UL19-1349 + CCG U CG CAG U CCCACACG AG 20 1589

HSV2-UL19-1350 + UCGCAGUCCCACACGAGGGG 20 1590

HSV2-UL19-1351 + ACGAGGGGCGGCAGCAGCGC 20 1591

HSV2-UL19-1352 + CGAGGGGCGGCAGCAGCGCC 20 1592

HSV2-UL19-1353 + GCAGCGCCGGGUCGCGCAUC 20 1593

HSV2-UL19-1354 + GCGCAUCAGGUGAUUCAGCU 20 1594

HSV2-UL19-1355 + UGAGCCUGCCCGCCCAGCUC 20 1595

HSV2-UL19-1356 + GAGCCUGCCCGCCCAGCUCC 20 1596

HSV2-UL19-1357 + UGCCCGCCCAGCUCCGGGCC 20 1597

HSV2-UL19-1358 + CGCCCAGCUCCGGGCCCGGC 20 1598

HSV2-UL19-1359 + GCCCAGCUCCGGGCCCGGCA 20 1599

HSV2-UL19-1360 + G G G U AAAG U CG U CCACCAG C 20 1600

HSV2-UL19-1361 + GGUAAAGUCGUCCACCAGCU 20 1601

HSV2-UL19-1362 + AGUCGUCCACCAGCUGGGCC 20 1602

HSV2-UL19-1363 + GUCGUCCACCAGCUGGGCCA 20 1603

HSV2-UL19-1364 + GCUGGGCCAGGGCCUCGACG 20 1604

HSV2-UL19-1365 + CUGGGCCAGGGCCUCGACGU 20 1605

HSV2-UL19-1366 + GGGCCUCGACGUGGGCCACC 20 1606

HSV2-UL19-1367 + GGCCACCAGGUCCCGAUACA 20 1607 HSV2-UL19-1368 + AUACACGGCCAUGCACUCCU 20 1608

HSV2-UL19-1369 + UACACGGCCAUGCACUCCUC 20 1609

HSV2-UL19-1370 + ACACGGCCAUGCACUCCUCG 20 1610

HSV2-UL19-1371 + CGGCCAUGCACUCCUCGGGG 20 1611

HSV2-UL19-1372 + CCUCGGGGAGGUCGCCCCCG 20 1612

HSV2-UL19-1373 + GGGGAGGUCGCCCCCGAGGU 20 1613

HSV2-UL19-1374 + UCGCGUGUUGUUCCAGUAGC 20 1614

HSV2-UL19-1375 + ACUGAGUCACGAGCCGCGCC 20 1615

HSV2-UL19-1376 + CUGAGUCACGAGCCGCGCCA 20 1616

HSV2-UL19-1377 + GUGCUCGUUGCCGUGAAUCG 20 1617

HSV2-UL19-1378 + CGUGAAUCGCGGCUUGCAGC 20 1618

HSV2-UL19-1379 + U G CAG CAG G U AAAACACCG C 20 1619

HSV2-UL19-1380 + GCAGCAGGUAAAACACCGCC 20 1620

HSV2-UL19-1381 + AAAACACCGCCGGGUAGCUG 20 1621

HSV2-UL19-1382 + UGCGGUCCUCGAACGCCCCG 20 1622

HSV2-UL19-1383 + G U CCU CG AACG CCCCG CG G A 20 1623

HSV2-UL19-1384 + CUCGAACGCCCCGCGGACGG 20 1624

HSV2-UL19-1385 + CGCCCCGCGGACGGCGGCUA 20 1625

HSV2-UL19-1386 + CGGACGGCGGCUAUGGUAGC 20 1626

HSV2-UL19-1387 + GGCUAUGGUAGCCGGCGCCA 20 1627

HSV2-UL19-1388 + UGGUAGCCGGCGCCAUGGCG 20 1628

HSV2-UL19-1389 + UAGCCGGCGCCAUGGCGUGG 20 1629

HSV2-UL19-1390 + GGCGGCCAACGCCGAGCUCC 20 1630

HSV2-UL19-1391 + CAACGCCGAGCUCCAGGCCC 20 1631

HSV2-UL19-1392 + AACGCCGAGCUCCAGGCCCC 20 1632

HSV2-UL19-1393 + CG G G CG U CACG AAACG CCAC 20 1633

HSV2-UL19-1394 + ACG CCACCG G ACACAG CG CC 20 1634

HSV2-UL19-1395 + CG CCACCG G ACACAG CG CCA 20 1635

HSV2-UL19-1396 + GCCACCGGACACAGCGCCAG 20 1636

HSV2-UL19-1397 + CCGGACACAGCGCCAGGGGC 20 1637

HSV2-UL19-1398 + GUUGCCGUUGACCACGCGCC 20 1638

HSV2-UL19-1399 + GCCGU UGACCACGCGCCAGG 20 1639

HSV2-UL19-1400 + UGACCACGCGCCAGGUGGCC 20 1640

HSV2-UL19-1401 + CAGGUGGCCUGGAUCGCCCC 20 1641

HSV2-UL19-1402 + GGCCUGGAUCGCCCCCGGAC 20 1642

HSV2-UL19-1403 + UGGAUCGCCCCCGGACCGGC 20 1643

HSV2-UL19-1404 + GGAUCGCCCCCGGACCGGCC 20 1644

HSV2-UL19-1405 + GAUCGCCCCCGGACCGGCCG 20 1645

HSV2-UL19-1406 + AUCGCCCCCGGACCGGCCGG 20 1646

HSV2-UL19-1407 + UCGCCCCCGGACCGGCCGGG 20 1647

HSV2-UL19-1408 + GGCCGGGGGGACUUCGCCGC 20 1648

HSV2-UL19-1409 + GCCGGGGGGACUUCGCCGCC 20 1649

HSV2-UL19-1410 + GCCGCCGGGAAGCUCGACGU 20 1650

HSV2-UL19-1411 + GCCCGCGAAGAAGUCGAACG 20 1651

HSV2-UL19-1412 + CCCG CG AAG AAG U CG AACGC 20 1652

HSV2-UL19-1413 + CCGCGAAGAAGUCGAACGCG 20 1653 HSV2-UL19-1414 + CGGGGUGCAGCUCCAGAGCC 20 1654

HSV2-UL19-1415 + GUGCAGCUCCAGAGCCAGGU 20 1655

HSV2-UL19-1416 + CAGAGCCAGGUUGGCGUUGU 20 1656

HSV2-UL19-1417 + AGAGCCAGGUUGGCGUUGUC 20 1657

HSV2-UL19-1418 + GGGCUGCAUGAACUGCUCCG 20 1658

HSV2-UL19-1419 + UGAACUGCUCCGCGGUCAUC 20 1659

HSV2-UL19-1420 + CUCCGCGGUCAUCUGGCACU 20 1660

HSV2-UL19-1421 + UCUGGCACUCGGCGACCCAC 20 1661

HSV2-UL19-1422 + ACUCGGCGACCCACCGGACC 20 1662

HSV2-UL19-1423 + CGACCCACCGGACCCGGCCG 20 1663

HSV2-UL19-1424 + GACCCACCGGACCCGGCCGU 20 1664

HSV2-UL19-1425 + ACCGGACCCGGCCGUGGGCG 20 1665

HSV2-UL19-1426 + GUGGGCGAGGCGCUGCCGCC 20 1666

HSV2-UL19-1427 + ACGCUGCUGCAUGUCCGCGC 20 1667

HSV2-UL19-1428 + CGCUGCUGCAUGUCCGCGCC 20 1668

HSV2-UL19-1429 + GCUGCUGCAUGUCCGCGCCG 20 1669

HSV2-UL19-1430 + CUGCAUGUCCGCGCCGGGGC 20 1670

HSV2-UL19-1431 + AUGUCCGCGCCGGGGCCGGC 20 1671

HSV2-UL19-1432 + UGUCCGCGCCGGGGCCGGCC 20 1672

HSV2-UL19-1433 + GUCCGCGCCGGGGCCGGCCG 20 1673

HSV2-UL19-1434 + GCCGCGACGUACGCCCCGUA 20 1674

HSV2-UL19-1435 + GUACGCCCCGUACGGAUUCG 20 1675

HSV2-UL19-1436 + GUACGGAUUCGCGGCCUCGA 20 1676

HSV2-UL19-1437 + UACGGAUUCGCGGCCUCGAC 20 1677

HSV2-UL19-1438 + ACGGAUUCGCGGCCUCGACG 20 1678

HSV2-UL19-1439 + UCGCGGCCUCGACGGGGUCG 20 1679

HSV2-UL19-1440 + GUCGUGGUUCACGCCCCCGA 20 1680

HSV2-UL19-1441 + GCGUCGAUGUUCAUGAGCGA 20 1681

HSV2-UL19-1442 + CAUGAGCGAAGGAUGACACA 20 1682

HSV2-UL19-1443 + GGUCCCGACCGCGU UCUCCA 20 1683

HSV2-UL19-1444 + CCAUGGACAGCCGCAGAACC 20 1684

HSV2-UL19-1445 + UGGACAGCCGCAGAACCUGG 20 1685

HSV2-UL19-1446 + UUCCCCAAAAAAACAGCUGC 20 1686

HSV2-UL19-1447 + UCCCCAAAAAAACAGCUGCC 20 1687

HSV2-UL19-1448 + CCCCAAAAAAACAGCUGCCG 20 1688

HSV2-UL19-1449 + CA AAAAAACAG CU G CCG G G G 20 1689

HSV2-UL19-1450 + AAAAAAACAGCUGCCGGGGA 20 1690

HSV2-UL19-1451 + GCUGCCGGGGAGGGAACGCG 20 1691

HSV2-UL19-1452 + CUGCCGGGGAGGGAACGCGC 20 1692

HSV2-UL19-1453 + UGCCGGGGAGGGAACGCGCG 20 1693

HSV2-UL19-1454 + GGAGGGAACGCGCGGGGCUC 20 1694

HSV2-UL19-1455 + GAGGGAACGCGCGGGGCUCC 20 1695

HSV2-UL19-1456 + GGAACGCGCGGGGCUCCGGG 20 1696

HSV2-UL19-1457 + CGCGCGGGGCUCCGGGUGGC 20 1697

HSV2-UL19-1458 + GCGCGGGGCUCCGGGUGGCC 20 1698

HSV2-UL19-1459 + CGCGGGGCUCCGGGUGGCCG 20 1699 HSV2-UL19-1460 + GCGGGGCUCCGGGUGGCCGG 20 1700

HSV2-UL19-1461 + GGGCUCCGGGUGGCCGGGGG 20 1701

HSV2-UL19-1462 + GGCUCCGGGUGGCCGGGGGC 20 1702

HSV2-UL19-1463 + GGUGGCCGGGGGCGGGCACC 20 1703

HSV2-UL19-1464 + GGGGGCGGGCACCAGGUCCC 20 1704

HSV2-UL19-1465 + CACCAGGUCCCCGGCGUGCG 20 1705

HSV2-UL19-1466 + GUGCGCGGCGAAGCGCUCCA 20 1706

HSV2-UL19-1467 + GCGGCGAAGCGCUCCAUGGC 20 1707

HSV2-UL19-1468 + CGGCGAAGCGCUCCAUGGCC 20 1708

HSV2-UL19-1469 + UGGCCGGGUUGAACAGCCCC 20 1709

HSV2-UL19-1470 + GGCCGGGUUGAACAG CCCCA 20 1710

HSV2-UL19-1471 + GCCGGGUUGAACAGCCCCAG 20 1711

HSV2-UL19-1472 + GGUUGAACAGCCCCAGGGGC 20 1712

HSV2-UL19-1473 + CCAGGGGCAGGACGAACGUC 20 1713

HSV2-UL19-1474 + CAGGACGAACG U CAG GU CCA 20 1714

HSV2-UL19-1475 + UCAGGUCCAUGGCGCCCACC 20 1715

HSV2-UL19-1476 + CAGGUCCAUGGCGCCCACCA 20 1716

HSV2-UL19-1477 + AGGUCCAUGGCGCCCACCAG 20 1717

HSV2-UL19-1478 + GGUCCAUGGCGCCCACCAGG 20 1718

HSV2-UL19-1479 + CAUGGCGCCCACCAGGGGGU 20 1719

HSV2-UL19-1480 + AUGGCGCCCACCAGGGGGUA 20 1720

HSV2-UL19-1481 + CACCAGGGGGUAGGGCACGU 20 1721

HSV2-UL19-1482 + CAGGGGGUAGGGCACGUUGG 20 1722

HSV2-UL19-1483 + GGGGUAGGGCACGUUGGUGG 20 1723

HSV2-UL19-1484 + CGGCGUAGAUGCGUCUCUCC 20 1724

HSV2-UL19-1485 + GGCGUAGAUGCGUCUCUCCA 20 1725

HSV2-UL19-1486 + UGCGUCUCUCCAGGGCCUCC 20 1726

HSV2-UL19-1487 + GAAGACCAGCCUGUCGCCUA 20 1727

HSV2-UL19-1488 + CCGCGCGCACGCGCGUUGUC 20 1728

HSV2-UL19-1489 + CGCGCGCACGCGCGUUGUCU 20 1729

HSV2-UL19-1490 + GCGCGCACGCGCGUUGUCUG 20 1730

HSV2-UL19-1491 + CGCGCACGCGCGUUGUCUGG 20 1731

HSV2-UL19-1492 + GCGCACGCGCGUUGUCUGGG 20 1732

HSV2-UL19-1493 + CGAGUUCAUCCAGCGUCUCC 20 1733

HSV2-UL19-1494 + GCU CCU CCU GCAU AUCCAGC 20 1734

HSV2-UL19-1495 + CCUCCUGCAUAUCCAGCAGG 20 1735

HSV2-UL19-1496 + CCUGCAUAUCCAGCAGGUGG 20 1736

HSV2-UL19-1497 + GGUGGCGGCCCACGUCGUCC 20 1737

HSV2-UL19-1498 + CACGUCGUCCAGGCUCCGCA 20 1738

HSV2-UL19-1499 + CCAU CACCAG CGCCG UGACG 20 1739

HSV2-UL19-1500 + CACCAGCGCCGUGACGAGGU 20 1740

HSV2-UL19-1501 + G U U CAAGACCAU CU CGCCG U 20 1741

HSV2-UL19-1502 + ACCAUCUCGCCGUAGGUCAC 20 1742

HSV2-UL19-1503 + GCCGUAGGUCACCGGCACGU 20 1743

HSV2-UL19-1504 + GGUCACCGGCACGUCGGCCU 20 1744

HSV2-UL19-1505 + CCUCGGUGUCCUCCACCUUC 20 1745 HSV2-UL19-1506 + G G U G U CCU CCACCU U CAGG A 20 1746

HSV2-UL19-1507 + CCACCUUCAGGAAGGACUGC 20 1747

HSV2-UL19-1508 + CCUUCAGGAAGGACUGCAGG 20 1748

HSV2-UL19-1509 + CUGCAGGAGGCGCUGUUUGA 20 1749

HSV2-UL19-1510 + CAGGAGGCGCUGUUUGAUGG 20 1750

HSV2-UL19-1511 + GAGGCGCUGUUUGAUGGCGG 20 1751

HSV2-UL19-1512 + GCGCUGUUUGAUGGCGGCGG 20 1752

HSV2-UL19-1513 + GUGACCAGCACCCCGUCGAC 20 1753

HSV2-UL19-1514 + GACCGGCCGCCCGCGCGUGU 20 1754

HSV2-UL19-1515 + CGCGCGUGUCGGCGUGCGUC 20 1755

HSV2-UL19-1516 + GCGUGUCGGCGUGCGUCAGG 20 1756

HSV2-UL19-1517 + CGUGUCGGCGUGCGUCAGGC 20 1757

HSV2-UL19-1518 + GUGUCGGCGUGCGUCAGGCG 20 1758

HSV2-UL19-1519 + GGCGUGCGUCAGGCGGGGCA 20 1759

HSV2-UL19-1520 + CGUCAGGCGGGGCACGGCCA 20 1760

HSV2-UL19-1521 + AGGCGGGGCACGGCCACGGA 20 1761

HSV2-UL19-1522 + CACGGACGGCUGCGUCGCCG 20 1762

HSV2-UL19-1523 + ACGGCUGCGUCGCCGUGGUC 20 1763

HSV2-UL19-1524 + CGUGGUCAGGUCCACGAGCC 20 1764

HSV2-UL19-1525 + GUCCACGAGCCAGGCCUCGA 20 1765

HSV2-UL19-1526 + GCCAGGCCUCGAUGGCCUCG 20 1766

HSV2-UL19-1527 + CCUCGAUGGCCUCGCGGCGA 20 1767

HSV2-UL19-1528 + GGCGAUGGCCCGCCUUGCCC 20 1768

HSV2-UL19-1529 + GCAAAAGCUCCGCUUCAGCU 20 1769

HSV2-UL19-1530 + UCCGCU UCAGCUCGGCGACC 20 1770

HSV2-UL19-1531 + CCGCUUCAGCUCGGCGACCA 20 1771

HSV2-UL19-1532 + GCUCGGCGACCAGGGUCGCC 20 1772

HSV2-UL19-1533 + CUCGGCGACCAGGGUCGCCC 20 1773

HSV2-UL19-1534 + CAGGGUCGCCCGGGCAACCC 20 1774

HSV2-UL19-1535 + CCCGGGCAACCCUGGUCGCC 20 1775

HSV2-UL19-1536 + GUUGUCGAGAUAUCGUUGCA 20 1776

HSV2-UL19-1537 + UUGUCGAGAUAUCGUUGCAU 20 1777

HSV2-UL19-1538 + AUCGUUGCAUGGGCAACAGC 20 1778

HSV2-UL19-1539 + UCGUUGCAUGGGCAACAGCA 20 1779

HSV2-UL19-1540 + GCAUGGGCAACAGCAGGGCC 20 1780

HSV2-UL19-1541 + CA U G G G CAACAG CAG G G CCA 20 1781

HSV2-UL19-1542 + AUGGGCAACAGCAGGGCCAG 20 1782

HSV2-UL19-1543 + G G CAACAG CAGGGCCAGGGG 20 1783

HSV2-UL19-1544 + CCAACAGCACGUGCAGCAUC 20 1784

HSV2-UL19-1545 + CAGCACGUGCAGCAUCUGGU 20 1785

HSV2-UL19-1546 + UGCCGCGCUCAAACGCCCCC 20 1786

HSV2-UL19-1547 + G CG CU CAAACG CCCCCAG G A 20 1787

HSV2-UL19-1548 + CAAACG CCCCCAG GACG G CC 20 1788

HSV2-UL19-1549 + AGAGACAGGCCCUUUAG 17 1789

HSV2-UL19-1550 + GAGACAGGCCCUUUAGC 17 1790

HSV2-UL19-1551 + AGACAGGCCCUUUAGCG 17 1791 HSV2-UL19-1552 + CAGGCCCUUUAGCGGGG 17 1792

HSV2-UL19-1553 + UGAGAUACUGCGUAAAG 17 1793

HSV2-UL19-1554 + GAGAUACUGCGUAAAGU 17 1794

HSV2-UL19-1555 + AGUGGGUCUCUCGCGCG 17 1795

HSV2-UL19-1556 + GUGGGUCUCUCGCGCGU 17 1796

HSV2-UL19-1557 + CGUGGGCCUCCCCAUCG 17 1797

HSV2-UL19-1558 + GUGGGCCUCCCCAUCGC 17 1798

HSV2-UL19-1559 + CGCGGGCGCUGCGUAGC 17 1799

HSV2-UL19-1560 + GCGGGCGCUGCGUAGCA 17 1800

HSV2-UL19-1561 + GGCGCUGCGUAGCAGGG 17 1801

HSV2-UL19-1562 + GCGCUGCGUAGCAGGGC 17 1802

HSV2-UL19-1563 + CGCUGCGUAGCAGGGCG 17 1803

HSV2-UL19-1564 + UAGCAGGGCGGGGUCGC 17 1804

HSV2-UL19-1565 + GGCGGGGUCGCUGGCGC 17 1805

HSV2-UL19-1566 + UCGCUGGCGCAGGUGAU 17 1806

HSV2-UL19-1567 + CGCUGGCGCAGGUGAUC 17 1807

HSV2-UL19-1568 + GGCGCAGGUGAUCGGGU 17 1808

HSV2-UL19-1569 + GGUAGGCUUCCUGAAAC 17 1809

HSV2-UL19-1570 + UCCUGAAACAGGCCGCA 17 1810

HSV2-UL19-1571 + CCUGAAACAGGCCGCAC 17 1811

HSV2-UL19-1572 + GGUCUUCCACGAGCUCG 17 1812

HSV2-UL19-1573 + ACGAGCUCGCGGCACCC 17 1813

HSV2-UL19-1574 + AGCUCGCGGCACCCCGG 17 1814

HSV2-UL19-1575 + GCUCGCGGCACCCCGGC 17 1815

HSV2-UL19-1576 + CUUAAACUGCACGUCGC 17 1816

HSV2-UL19-1577 + CUGCACGUCGCUGGCAG 17 1817

HSV2-UL19-1578 + CACGUCGCUGGCAGCGG 17 1818

HSV2-UL19-1579 + GCUGGCAGCGGUGGCCG 17 1819

HSV2-UL19-1580 + CCACGAUGAGACGCUCC 17 1820

HSV2-UL19-1581 + CUCCAGGCAGCGAUGUU 17 1821

HSV2-UL19-1582 + AUGUUUGGCCGUGAUGU 17 1822

HSV2-UL19-1583 + GGCCGUGAUGUCGGCCG 17 1823

HSV2-UL19-1584 + GGUGAAGAACUUGAAGC 17 1824

HSV2-UL19-1585 + G UGAAGAACU UGAAGCA 17 1825

HSV2-UL19-1586 + UGAAGAACUUGAAGCAG 17 1826

HSV2-UL19-1587 + ACUUGAAGCAGGGGCUG 17 1827

HSV2-UL19-1588 + GAAGCAGGGGCUGAGGA 17 1828

HSV2-UL19-1589 + AAGCAGGGGCUGAGGAC 17 1829

HSV2-UL19-1590 + GGGGCUGAGGACGGGCG 17 1830

HSV2-UL19-1591 + CGGGCGAGGCCCCGUUG 17 1831

HSV2-UL19-1592 + GGCCCCGUUGAGGUGAU 17 1832

HSV2-UL19-1593 + AGGCCCCGUUGUACAGC 17 1833

HSV2-UL19-1594 + AACCGCUGCGACGCCCA 17 1834

HSV2-UL19-1595 + ACCG CUGCGACG CCCAC 17 1835

HSV2-UL19-1596 + CUGCGACGCCCACGGGU 17 1836

HSV2-UL19-1597 + CGCCCACGGGUUGGCCG 17 1837 HSV2-UL19-1598 + GUUGGCCGUGGCCGCGA 17 1838

HSV2-UL19-1599 + UUGGCCGUGGCCGCGAA 17 1839

HSV2-UL19-1600 + GCCGCGAAGGGCCGCGC 17 1840

HSV2-UL19-1601 + CCGCGAAGGGCCGCGCC 17 1841

HSV2-UL19-1602 + GCCGCGCCGGGUCGCUC 17 1842

HSV2-UL19-1603 + CCGGGUCGCUCUGGCCG 17 1843

HSV2-UL19-1604 + GGCCGUGGUCGUACAUG 17 1844

HSV2-UL19-1605 + GCCGUGGUCGUACAUGA 17 1845

HSV2-UL19-1606 + CCCCGCGUACACGCCGC 17 1846

HSV2-UL19-1607 + CCGGCCGCGCGUCCCCG 17 1847

HSV2-UL19-1608 + CGGCCGCGCGUCCCCGC 17 1848

HSV2-UL19-1609 + GCGUCCCCGCGGGUUGC 17 1849

HSV2-UL19-1610 + CGUCCCCGCGGGUUGCA 17 1850

HSV2-UL19-1611 + CCCGCGGGUUGCAGGGC 17 1851

HSV2-UL19-1612 + AAAG U AG U U G AU G U CCG 17 1852

HSV2-UL19-1613 + GUUGAUGUCCGUGGCCA 17 1853

HSV2-UL19-1614 + UUGAUGUCCGUGGCCAC 17 1854

HSV2-UL19-1615 + UGAUGUCCGUGGCCACG 17 1855

HSV2-UL19-1616 + GAUGUCCGUGGCCACGG 17 1856

HSV2-UL19-1617 + GUCCGUGGCCACGGGGG 17 1857

HSV2-UL19-1618 + GGCGAUGAACUCACACA 17 1858

HSV2-UL19-1619 + ACACGGCAUCCUGCCCG 17 1859

HSV2-UL19-1620 + CUGCCCGUGGUCCAUGC 17 1860

HSV2-UL19-1621 + UCCAUGCCGGCGCGCCG 17 1861

HSV2-UL19-1622 + CGGCGCGCCGCGGCACC 17 1862

HSV2-UL19-1623 + GGCGCGCCGCGGCACCU 17 1863

HSV2-UL19-1624 + UGGGCGCAGCCAAAGAC 17 1864

HSV2-UL19-1625 + GGGCGCAGCCAAAGACC 17 1865

HSV2-UL19-1626 + CGCAGCCAAAGACCGGG 17 1866

HSV2-UL19-1627 + GCAGCCAAAGACCGGGA 17 1867

HSV2-UL19-1628 + CAG CCAAAG ACCGG G AG 17 1868

HSV2-UL19-1629 + CAAAGACCGGGAGGGGC 17 1869

HSV2-UL19-1630 + AAAGACCGGGAGGGGCU 17 1870

HSV2-UL19-1631 + ACCGGGAGGGGCUGGGC 17 1871

HSV2-UL19-1632 + GCUGGGCCGGCCCCAGC 17 1872

HSV2-UL19-1633 + CCACGACCGCGUUGCGC 17 1873

HSV2-UL19-1634 + CGCGUUGCGCAGGUACA 17 1874

HSV2-UL19-1635 + GUUGCGCAGGUACACGG 17 1875

HSV2-UL19-1636 + GCCGCGUUGUCUAGCAG 17 1876

HSV2-UL19-1637 + CCGCGUUGUCUAGCAGC 17 1877

HSV2-UL19-1638 + CGCGUUGUCUAGCAGCG 17 1878

HSV2-UL19-1639 + GCGUUGUCUAGCAGCGG 17 1879

HSV2-UL19-1640 + CGUUGUCUAGCAGCGGG 17 1880

HSV2-UL19-1641 + GUUGUCUAGCAGCGGGG 17 1881

HSV2-UL19-1642 + GCAGCGGGGGGGCCCCG 17 1882

HSV2-UL19-1643 + GGGGGGCCCCGCGGCCG 17 1883 HSV2-UL19-1644 + GGCCGAGGUAAAAGUUU 17 1884

HSV2-UL19-1645 + GCCGAGGUAAAAGUUUU 17 1885

HSV2-UL19-1646 + CCGAGGUAAAAGUUUUG 17 1886

HSV2-UL19-1647 + CGAGGUAAAAGUUUUGG 17 1887

HSV2-UL19-1648 + GGUAAAAGUUUUGGGGG 17 1888

HSV2-UL19-1649 + GUUGCCCAUGUCCGUAA 17 1889

HSV2-UL19-1650 + UUGCCCAUGUCCGUAAC 17 1890

HSV2-UL19-1651 + UGCCCAUGUCCGUAACG 17 1891

HSV2-UL19-1652 + UGUCCGUAACGGGGUUG 17 1892

HSV2-UL19-1653 + CGUAACGGGGUUGCGGA 17 1893

HSV2-UL19-1654 + GUUGCGGACGGUGCCCG 17 1894

HSV2-UL19-1655 + GGUGCCCGUGGCCGCGA 17 1895

HSV2-UL19-1656 + GCCCGUGGCCGCGACGG 17 1896

HSV2-UL19-1657 + CGGUGUAGCCCACACCC 17 1897

HSV2-UL19-1658 + AGGUCCACGUUUCCGCG 17 1898

HSV2-UL19-1659 + CCACGUUUCCGCGCGGC 17 1899

HSV2-UL19-1660 + CACGUUUCCGCGCGGCU 17 1900

HSV2-UL19-1661 + CCCCCCCGCCCGUUUCG 17 1901

HSV2-UL19-1662 + CCCCGCCCGUUUCGUGG 17 1902

HSV2-UL19-1663 + CCCGCCCGUUUCGUGGC 17 1903

HSV2-UL19-1664 + UUUCGUGGCGGGCCACC 17 1904

HSV2-UL19-1665 + GGCGGGCCACCUGGAGC 17 1905

HSV2-UL19-1666 + CGCCUCCGACGCGCGCU 17 1906

HSV2-UL19-1667 + G G AAAACAGCACG U U CU 17 1907

HSV2-UL19-1668 + CG U U CU CGG U CACGAAG 17 1908

HSV2-UL19-1669 + GCGGUCCUGCCGCACGA 17 1909

HSV2-UL19-1670 + GACGGUGAACCCGAACC 17 1910

HSV2-UL19-1671 + ACGGUGAACCCGAACCC 17 1911

HSV2-UL19-1672 + CGGUGAACCCGAACCCG 17 1912

HSV2-UL19-1673 + UGAACCCGAACCCGGGG 17 1913

HSV2-UL19-1674 + ACCCGAACCCGGGGUGG 17 1914

HSV2-UL19-1675 + GGAGGCCCGUCUUGAGC 17 1915

HSV2-UL19-1676 + UCUUGAGCUGGUGAUAC 17 1916

HSV2-UL19-1677 + CUUGAGCUGGUGAUACA 17 1917

HSV2-UL19-1678 + CUGGUGAUACAGGGCCA 17 1918

HSV2-UL19-1679 + UGGUGAUACAGGGCCAC 17 1919

HSV2-UL19-1680 + GGUGAUACAGGGCCACG 17 1920

HSV2-UL19-1681 + CCCCG CCAU G AG CGCG U 17 1921

HSV2-UL19-1682 + CCCCCGCCGCGCUCUCG 17 1922

HSV2-UL19-1683 + CCCCGCCGCGCUCUCGC 17 1923

HSV2-UL19-1684 + GCGGGCGUGCUGCACCA 17 1924

HSV2-UL19-1685 + CGGGCGUGCUGCACCAC 17 1925

HSV2-UL19-1686 + CGUGCUGCACCACGGGC 17 1926

HSV2-UL19-1687 + GCUGCACCACGGGCUGG 17 1927

HSV2-UL19-1688 + CACCACGGGCUGGCGGA 17 1928

HSV2-UL19-1689 + CACGGGCUGGCGGAUGG 17 1929 HSV2-UL19-1690 + GAUGGAGGAGAAGUAGU 17 1930

HSV2-UL19-1691 + AGAAGUAGUUGGCCCCC 17 1931

HSV2-UL19-1692 + GAAGUAGUUGGCCCCCA 17 1932

HSV2-UL19-1693 + UAGUUGGCCCCCAGGGC 17 1933

HSV2-UL19-1694 + AGUUGGCCCCCAGGGCC 17 1934

HSV2-UL19-1695 + GUUGGCCCCCAGGGCCG 17 1935

HSV2-UL19-1696 + UUGGCCCCCAGGGCCGG 17 1936

HSV2-UL19-1697 + UGGCCCCCAGGGCCGGG 17 1937

HSV2-UL19-1698 + CCAGGGCCGGGGGGACC 17 1938

HSV2-UL19-1699 + CAGGGCCGGGGGGACCA 17 1939

HSV2-UL19-1700 + AGGGCCGGGGGGACCAG 17 1940

HSV2-UL19-1701 + GGGCCGGGGGGACCAGG 17 1941

HSV2-UL19-1702 + GGGGGACGUCGCGCGCC 17 1942

HSV2-UL19-1703 + CGCGCGCCAGGUCGCGC 17 1943

HSV2-UL19-1704 + GCGCGCCAGGUCGCGCA 17 1944

HSV2-UL19-1705 + GCCAGGUCGCGCAGGGC 17 1945

HSV2-UL19-1706 + CCAGGUCGCGCAGGGCC 17 1946

HSV2-UL19-1707 + CAGGUCGCGCAGGGCCG 17 1947

HSV2-UL19-1708 + AGGUCGCGCAGGGCCGG 17 1948

HSV2-UL19-1709 + GGUCGCGCAGGGCCGGG 17 1949

HSV2-UL19-1710 + CAGGGCCGGGGGGAAGU 17 1950

HSV2-UL19-1711 + AGGGCCGGGGGGAAGUU 17 1951

HSV2-UL19-1712 + GGGGAAGUUGGGCGCGU 17 1952

HSV2-UL19-1713 + UGGGCGCGUUGGCCACG 17 1953

HSV2-UL19-1714 + CGCGUUGGCCACGUGGU 17 1954

HSV2-UL19-1715 + CG CCCG CAAACAG CG CG 17 1955

HSV2-UL19-1716 + CGCAAACAGCGCGUGGA 17 1956

HSV2-UL19-1717 + GCAAACAGCGCGUGGAC 17 1957

HSV2-UL19-1718 + ACAGCGCGUGGACGGGC 17 1958

HSV2-UL19-1719 + AGAAGUAUUCGCCAUUU 17 1959

HSV2-UL19-1720 + GUAUUCGCCAUUUUGGA 17 1960

HSV2-UL19-1721 + CGCCAUUUUGGAUGGUG 17 1961

HSV2-UL19-1722 + UUUGGAUGGUGUGGUCC 17 1962

HSV2-UL19-1723 + UGGUGUGGUCCAGGUGC 17 1963

HSV2-UL19-1724 + GGUGUGGUCCAGGUGCU 17 1964

HSV2-UL19-1725 + GUGUGGUCCAGGUGCUG 17 1965

HSV2-UL19-1726 + UGUGGUCCAGGUGCUGG 17 1966

HSV2-UL19-1727 + GUGGUCCAGGUGCUGGG 17 1967

HSV2-UL19-1728 + GGCCAUGAGCAGCACGC 17 1968

HSV2-UL19-1729 + GUCGAAGAUGCGCAUGU 17 1969

HSV2-UL19-1730 + CAUGUUGGCCGUCGACG 17 1970

HSV2-UL19-1731 + GGCCGUCGACGCGGUGU 17 1971

HSV2-UL19-1732 + GGUGUUGGCGCCCGCGU 17 1972

HSV2-UL19-1733 + GUGUUGGCGCCCGCGUC 17 1973

HSV2-UL19-1734 + GCCCGCGUCGGGCGCCG 17 1974

HSV2-UL19-1735 + CAGCGCCGUCGUGCGCU 17 1975 HSV2-UL19-1736 + CACCUGCAGCGUGAGCA 17 1976

HSV2-UL19-1737 + CUGCAGCGUGAGCAUGG 17 1977

HSV2-UL19-1738 + UGCAGCGUGAGCAUGGC 17 1978

HSV2-UL19-1739 + CGACGACGCGCCCGUUG 17 1979

HSV2-UL19-1740 + CAUGCGCUUGACCGUGU 17 1980

HSV2-UL19-1741 + CGUGUUGGCCACCAGAU 17 1981

HSV2-UL19-1742 + G U U GG CCACCAG AU U G G 17 1982

HSV2-UL19-1743 + UUGGCCACCAGAUUGGC 17 1983

HSV2-UL19-1744 + AGAUUGGCGGGAUGCAG 17 1984

HSV2-UL19-1745 + GAUUGGCGGGAUGCAGC 17 1985

HSV2-UL19-1746 + UGGCGGGAUGCAGCGGG 17 1986

HSV2-UL19-1747 + GGCGGGAUGCAGCGGGU 17 1987

HSV2-UL19-1748 + GGGAUGCAGCGGGUGGG 17 1988

HSV2-UL19-1749 + GGAUGCAGCGGGUGGGC 17 1989

HSV2-UL19-1750 + GAUGCAGCGGGUGGGCG 17 1990

HSV2-UL19-1751 + CAGCGGGUGGGCGGGGU 17 1991

HSV2-UL19-1752 + GUGGGCGGGGUCGGUCA 17 1992

HSV2-UL19-1753 + UGGGCGGGGUCGGUCAC 17 1993

HSV2-UL19-1754 + UCGGUCACGGGAUCGCU 17 1994

HSV2-UL19-1755 + CGGUCACGGGAUCGCUC 17 1995

HSV2-UL19-1756 + GCUCGGGCACUCCUCAC 17 1996

HSV2-UL19-1757 + CUCGGGCACUCCUCACC 17 1997

HSV2-UL19-1758 + UCGGGCACUCCUCACCG 17 1998

HSV2-UL19-1759 + CGGGCACUCCUCACCGG 17 1999

HSV2-UL19-1760 + UCACCGGGGGCGAUCUC 17 2000

HSV2-UL19-1761 + CACCGGGGGCGAUCUCC 17 2001

HSV2-UL19-1762 + CACCAUGUUCUGCAGCG 17 2002

HSV2-UL19-1763 + GCAGCGUGGCGUACACG 17 2003

HSV2-UL19-1764 + CGUACACGCGGUCGAAG 17 2004

HSV2-UL19-1765 + GUCGAAGCGGACCCCCG 17 2005

HSV2-UL19-1766 + GCAGCGCCCCCGCGAGA 17 2006

HSV2-UL19-1767 + CGCCCCCGCGAGAAGGC 17 2007

HSV2-UL19-1768 + CGUAAUAGUAGAUUUUG 17 2008

HSV2-UL19-1769 + AAUAGUAGAUUUUGUGG 17 2009

HSV2-UL19-1770 + GUAGAUUUUGUGGUGGA 17 2010

HSV2-UL19-1771 + GUGGUGGACGGUCCAGU 17 2011

HSV2-UL19-1772 + UGGACGGUCCAGUCGGC 17 2012

HSV2-UL19-1773 + CGGUCCAGUCGGCCGGC 17 2013

HSV2-UL19-1774 + CAGUCGGCCGGCCGGUG 17 2014

HSV2-UL19-1775 + CGGCCGGCCGGUGCGGC 17 2015

HSV2-UL19-1776 + CCGGUGCGGCCGGUCGU 17 2016

HSV2-UL19-1777 + GUGCGGCCGGUCGUCGG 17 2017

HSV2-UL19-1778 + CCGGUCGUCGGCGGCGU 17 2018

HSV2-UL19-1779 + CGGCGGCGUCGGCCGCG 17 2019

HSV2-UL19-1780 + GGCGGCGUCGGCCGCGC 17 2020

HSV2-UL19-1781 + CGUCGGCCGCGCGGGCC 17 2021 HSV2-UL19-1782 + GUCGGCCGCGCGGGCCU 17 2022

HSV2-UL19-1783 + GGGUGUUGUGCAGCAGC 17 2023

HSV2-UL19-1784 + GCAGCCGGCCGUCGUUG 17 2024

HSV2-UL19-1785 + GUCGUUGCGGUUAAAGU 17 2025

HSV2-UL19-1786 + GCACGCCGCCGCGUAGA 17 2026

HSV2-UL19-1787 + CACGCCGCCGCGUAGAC 17 2027

HSV2-UL19-1788 + GCCCCCCCGCGUCAAUC 17 2028

HSV2-UL19-1789 + CGUCAAUCCGGCAGUCU 17 2029

HSV2-UL19-1790 + CAAUCCGGCAGUCUCGG 17 2030

HSV2-UL19-1791 + UCCGGCAGUCUCGGUGG 17 2031

HSV2-UL19-1792 + AGUCUCGGUGGCGGUCC 17 2032

HSV2-UL19-1793 + GUCUCGGUGGCGGUCCA 17 2033

HSV2-UL19-1794 + GGGCCGCGUGUCGCAUA 17 2034

HSV2-UL19-1795 + CG U CG CAG U CCCACACG 17 2035

HSV2-UL19-1796 + G U CG CAG U CCCACACG A 17 2036

HSV2-UL19-1797 + UCGCAGUCCCACACGAG 17 2037

HSV2-UL19-1798 + CAGUCCCACACGAGGGG 17 2038

HSV2-UL19-1799 + AGGGGCGGCAGCAGCGC 17 2039

HSV2-UL19-1800 + GGGGCGGCAGCAGCGCC 17 2040

HSV2-UL19-1801 + GCGCCGGGUCGCGCAUC 17 2041

HSV2-UL19-1802 + CAUCAGGUGAUUCAGCU 17 2042

HSV2-UL19-1803 + GCCUGCCCGCCCAGCUC 17 2043

HSV2-UL19-1804 + CCUGCCCGCCCAGCUCC 17 2044

HSV2-UL19-1805 + CCGCCCAGCUCCGGGCC 17 2045

HSV2-UL19-1806 + CCAGCUCCGGGCCCGGC 17 2046

HSV2-UL19-1807 + CAGCUCCGGGCCCGGCA 17 2047

HSV2-UL19-1808 + UAAAGUCGUCCACCAGC 17 2048

HSV2-UL19-1809 + AAAG UCGUCCACCAGCU 17 2049

HSV2-UL19-1810 + CGUCCACCAGCUGGGCC 17 2050

HSV2-UL19-1811 + GUCCACCAGCUGGGCCA 17 2051

HSV2-UL19-1812 + GGGCCAGGGCCUCGACG 17 2052

HSV2-UL19-1813 + GGCCAGGGCCUCGACGU 17 2053

HSV2-UL19-1814 + CCUCGACGUGGGCCACC 17 2054

HSV2-UL19-1815 + CACCAGGUCCCGAUACA 17 2055

HSV2-UL19-1816 + CACGGCCAUGCACUCCU 17 2056

HSV2-UL19-1817 + ACGGCCAUGCACUCCUC 17 2057

HSV2-UL19-1818 + CGGCCAUGCACUCCUCG 17 2058

HSV2-UL19-1819 + CCAUGCACUCCUCGGGG 17 2059

HSV2-UL19-1820 + CGGGGAGGUCGCCCCCG 17 2060

HSV2-UL19-1821 + GAGGUCGCCCCCGAGGU 17 2061

HSV2-UL19-1822 + CGUGUUGUUCCAGUAGC 17 2062

HSV2-UL19-1823 + GAGUCACGAGCCGCGCC 17 2063

HSV2-UL19-1824 + AGUCACGAGCCGCGCCA 17 2064

HSV2-UL19-1825 + CUCGUUGCCGUGAAUCG 17 2065

HSV2-UL19-1826 + GAAUCGCGGCUUGCAGC 17 2066

HSV2-UL19-1827 + AGCAGGUAAAACACCGC 17 2067 HSV2-UL19-1828 + G CAG G U AAAACACCG CC 17 2068

HSV2-UL19-1829 + ACACCGCCGGGUAGCUG 17 2069

HSV2-UL19-1830 + GGUCCUCGAACGCCCCG 17 2070

HSV2-UL19-1831 + CUCGAACGCCCCGCGGA 17 2071

HSV2-UL19-1832 + GAACGCCCCGCGGACGG 17 2072

HSV2-UL19-1833 + CCCGCGGACGGCGGCUA 17 2073

HSV2-UL19-1834 + ACGGCGGCUAUGGUAGC 17 2074

HSV2-UL19-1835 + UAUGGUAGCCGGCGCCA 17 2075

HSV2-UL19-1836 + UAGCCGGCGCCAUGGCG 17 2076

HSV2-UL19-1837 + CCGGCGCCAUGGCGUGG 17 2077

HSV2-UL19-1838 + GGCCAACGCCGAGCUCC 17 2078

HSV2-UL19-1839 + CGCCGAGCUCCAGGCCC 17 2079

HSV2-UL19-1840 + GCCGAGCUCCAGGCCCC 17 2080

HSV2-UL19-1841 + GCGUCACGAAACGCCAC 17 2081

HSV2-UL19-1842 + CCACCGGACACAGCGCC 17 2082

HSV2-UL19-1843 + CACCGGACACAGCGCCA 17 2083

HSV2-UL19-1844 + ACCG G ACACAG CG CCAG 17 2084

HSV2-UL19-1845 + GACACAGCGCCAGGGGC 17 2085

HSV2-UL19-1846 + GCCGUUGACCACGCGCC 17 2086

HSV2-UL19-1847 + GUUGACCACGCGCCAGG 17 2087

HSV2-UL19-1848 + CCACGCGCCAGGUGGCC 17 2088

HSV2-UL19-1849 + GUGGCCUGGAUCGCCCC 17 2089

HSV2-UL19-1850 + CUGGAUCGCCCCCGGAC 17 2090

HSV2-UL19-1851 + AUCGCCCCCGGACCGGC 17 2091

HSV2-UL19-1852 + UCGCCCCCGGACCGGCC 17 2092

HSV2-UL19-1853 + CGCCCCCGGACCGGCCG 17 2093

HSV2-UL19-1854 + GCCCCCGGACCGGCCGG 17 2094

HSV2-UL19-1855 + CCCCCGGACCGGCCGGG 17 2095

HSV2-UL19-1856 + CGGGGGGACUUCGCCGC 17 2096

HSV2-UL19-1857 + GGGGGGACUUCGCCGCC 17 2097

HSV2-UL19-1858 + GCCGGGAAGCUCGACGU 17 2098

HSV2-UL19-1859 + CGCGAAGAAGUCGAACG 17 2099

HSV2-UL19-1860 + GCGAAGAAGUCGAACGC 17 2100

HSV2-UL19-1861 + CGAAGAAGUCGAACGCG 17 2101

HSV2-UL19-1862 + GGUGCAGCUCCAGAGCC 17 2102

HSV2-UL19-1863 + CAGCUCCAGAGCCAGGU 17 2103

HSV2-UL19-1864 + AGCCAGGUUGGCGUUGU 17 2104

HSV2-UL19-1865 + GCCAGGUUGGCGUUGUC 17 2105

HSV2-UL19-1866 + CUGCAUGAACUGCUCCG 17 2106

HSV2-UL19-1867 + ACUGCUCCGCGGUCAUC 17 2107

HSV2-UL19-1868 + CGCGGUCAUCUGGCACU 17 2108

HSV2-UL19-1869 + GGCACUCGGCGACCCAC 17 2109

HSV2-UL19-1870 + CGGCGACCCACCGGACC 17 2110

HSV2-UL19-1871 + CCCACCGGACCCGGCCG 17 2111

HSV2-UL19-1872 + CCACCGGACCCGGCCGU 17 2112

HSV2-UL19-1873 + GGACCCGGCCGUGGGCG 17 2113 HSV2-UL19-1874 + GGCGAGGCGCUGCCGCC 17 2114

HSV2-UL19-1875 + CUGCUGCAUGUCCGCGC 17 2115

HSV2-UL19-1876 + UGCUGCAUGUCCGCGCC 17 2116

HSV2-UL19-1877 + GCUGCAUGUCCGCGCCG 17 2117

HSV2-UL19-1878 + CAUGUCCGCGCCGGGGC 17 2118

HSV2-UL19-1879 + UCCGCGCCGGGGCCGGC 17 2119

HSV2-UL19-1880 + CCGCGCCGGGGCCGGCC 17 2120

HSV2-UL19-1881 + CGCGCCGGGGCCGGCCG 17 2121

HSV2-UL19-1882 + GCGACGUACGCCCCGUA 17 2122

HSV2-UL19-1883 + CGCCCCGUACGGAUUCG 17 2123

HSV2-UL19-1884 + CGGAUUCGCGGCCUCGA 17 2124

HSV2-UL19-1885 + GGAUUCGCGGCCUCGAC 17 2125

HSV2-UL19-1886 + GAUUCGCGGCCUCGACG 17 2126

HSV2-UL19-1887 + CGGCCUCGACGGGGUCG 17 2127

HSV2-UL19-1888 + GUGGUUCACGCCCCCGA 17 2128

HSV2-UL19-1889 + UCGAUGUUCAUGAGCGA 17 2129

HSV2-UL19-1890 + GAGCGAAGGAUGACACA 17 2130

HSV2-UL19-1891 + CCCGACCGCGUUCUCCA 17 2131

HSV2-UL19-1892 + UGGACAGCCGCAGAACC 17 2132

HSV2-UL19-1893 + ACAGCCGCAGAACCUGG 17 2133

HSV2-UL19-1894 + CCCAAA AAAAC AG CU G C 17 2134

HSV2-UL19-1895 + CCAA AAAAACAG CU G CC 17 2135

HSV2-UL19-1896 + CA AAAAAACAG CU G CCG 17 2136

HSV2-UL19-1897 + AAAAACAG CU G CCG G G G 17 2137

HSV2-UL19-1898 + AAAACAG CU G CCG G G G A 17 2138

HSV2-UL19-1899 + GCCGGGGAGGGAACGCG 17 2139

HSV2-UL19-1900 + CCGGGGAGGGAACGCGC 17 2140

HSV2-UL19-1901 + CGGGGAGGGAACGCGCG 17 2141

HSV2-UL19-1902 + GGGAACGCGCGGGGCUC 17 2142

HSV2-UL19-1903 + GGAACGCGCGGGGCUCC 17 2143

HSV2-UL19-1904 + ACGCGCGGGGCUCCGGG 17 2144

HSV2-UL19-1905 + GCGGGGCUCCGGGUGGC 17 2145

HSV2-UL19-1906 + CGGGGCUCCGGGUGGCC 17 2146

HSV2-UL19-1907 + GGGGCUCCGGGUGGCCG 17 2147

HSV2-UL19-1908 + GGGCUCCGGGUGGCCGG 17 2148

HSV2-UL19-1909 + CUCCGGGUGGCCGGGGG 17 2149

HSV2-UL19-1910 + UCCGGGUGGCCGGGGGC 17 2150

HSV2-UL19-1911 + GGCCGGGGGCGGGCACC 17 2151

HSV2-UL19-1912 + GGCGGGCACCAGGUCCC 17 2152

HSV2-UL19-1913 + CAGGUCCCCGGCGUGCG 17 2153

HSV2-UL19-1914 + CGCGGCGAAGCGCUCCA 17 2154

HSV2-UL19-1915 + GCGAAGCGCUCCAUGGC 17 2155

HSV2-UL19-1916 + CGAAGCGCUCCAUGGCC 17 2156

HSV2-UL19-1917 + CCGGGUUGAACAGCCCC 17 2157

HSV2-UL19-1918 + CGGGUUGAACAGCCCCA 17 2158

HSV2-UL19-1919 + GGGUUGAACAGCCCCAG 17 2159 HSV2-UL19-1920 + UGAACAGCCCCAGGGGC 17 2160

HSV2-UL19-1921 + GGGGCAGGACGAACGUC 17 2161

HSV2-UL19-1922 + GACGAACGUCAGGUCCA 17 2162

HSV2-UL19-1923 + GGUCCAUGGCGCCCACC 17 2163

HSV2-UL19-1924 + GUCCAUGGCGCCCACCA 17 2164

HSV2-UL19-1925 + UCCAUGGCGCCCACCAG 17 2165

HSV2-UL19-1926 + CCAUGGCGCCCACCAGG 17 2166

HSV2-UL19-1927 + GGCGCCCACCAGGGGGU 17 2167

HSV2-UL19-1928 + GCGCCCACCAGGGGGUA 17 2168

HSV2-UL19-1929 + CAGGGGGUAGGGCACGU 17 2169

HSV2-UL19-1930 + GGGGUAGGGCACGUUGG 17 2170

HSV2-UL19-1931 + GUAGGGCACGUUGGUGG 17 2171

HSV2-UL19-1932 + CGUAGAUGCGUCUCUCC 17 2172

HSV2-UL19-1933 + GUAGAUGCGUCUCUCCA 17 2173

HSV2-UL19-1934 + GUCUCUCCAGGGCCUCC 17 2174

HSV2-UL19-1935 + GACCAGCCUGUCGCCUA 17 2175

HSV2-UL19-1936 + CGCGCACGCGCGUUGUC 17 2176

HSV2-UL19-1937 + GCGCACGCGCGUUGUCU 17 2177

HSV2-UL19-1938 + CGCACGCGCGUUGUCUG 17 2178

HSV2-UL19-1939 + GCACGCGCGUUGUCUGG 17 2179

HSV2-UL19-1940 + CACGCGCGUUGUCUGGG 17 2180

HSV2-UL19-1941 + G U U CAU CCAGCG U CU CC 17 2181

HSV2-UL19-1942 + CCUCCUGCAUAUCCAGC 17 2182

HSV2-UL19-1943 + CCUGCAUAUCCAGCAGG 17 2183

HSV2-UL19-1944 + GCAUAUCCAGCAGGUGG 17 2184

HSV2-UL19-1945 + GGCGGCCCACGUCGUCC 17 2185

HSV2-UL19-1946 + GUCGUCCAGGCUCCGCA 17 2186

HSV2-UL19-1947 + UCACCAGCGCCGUGACG 17 2187

HSV2-UL19-1948 + CAGCGCCGUGACGAGGU 17 2188

HSV2-UL19-1949 + CAAGACCAUCUCGCCGU 17 2189

HSV2-UL19-1950 + AUCUCGCCGUAGGUCAC 17 2190

HSV2-UL19-1951 + GUAGGUCACCGGCACGU 17 2191

HSV2-UL19-1952 + CACCGGCACGUCGGCCU 17 2192

HSV2-UL19-1953 + CGGUGUCCUCCACCUUC 17 2193

HSV2-UL19-1954 + GUCCUCCACCUUCAGGA 17 2194

HSV2-UL19-1955 + CCUUCAGGAAGGACUGC 17 2195

HSV2-UL19-1956 + UCAGGAAGGACUGCAGG 17 2196

HSV2-UL19-1957 + CAGGAGGCGCUGUUUGA 17 2197

HSV2-UL19-1958 + GAGGCGCUGUUUGAUGG 17 2198

HSV2-UL19-1959 + GCGCUGUUUGAUGGCGG 17 2199

HSV2-UL19-1960 + CUGUUUGAUGGCGGCGG 17 2200

HSV2-UL19-1961 + ACCAG CACCCCG U CG AC 17 2201

HSV2-UL19-1962 + CGGCCGCCCGCGCGUGU 17 2202

HSV2-UL19-1963 + GCGUGUCGGCGUGCGUC 17 2203

HSV2-UL19-1964 + UGUCGGCGUGCGUCAGG 17 2204

HSV2-UL19-1965 + GUCGGCGUGCGUCAGGC 17 2205 HSV2-UL19-1966 + UCGGCGUGCGUCAGGCG 17 2206

HSV2-UL19-1967 + GUGCGUCAGGCGGGGCA 17 2207

HSV2-UL19-1968 + CAGGCGGGGCACGGCCA 17 2208

HSV2-UL19-1969 + CGGGGCACGGCCACGGA 17 2209

HSV2-UL19-1970 + GGACGGCUGCGUCGCCG 17 2210

HSV2-UL19-1971 + GCUGCGUCGCCGUGGUC 17 2211

HSV2-UL19-1972 + GGUCAGGUCCACGAGCC 17 2212

HSV2-UL19-1973 + CACGAGCCAGGCCUCGA 17 2213

HSV2-UL19-1974 + AGGCCUCGAUGGCCUCG 17 2214

HSV2-UL19-1975 + CGAUGGCCUCGCGGCGA 17 2215

HSV2-UL19-1976 + GAUGGCCCGCCUUGCCC 17 2216

HSV2-UL19-1977 + AAAGCUCCGCUUCAGCU 17 2217

HSV2-UL19-1978 + GCU UCAGCUCGGCGACC 17 2218

HSV2-UL19-1979 + CU UCAGCUCGGCGACCA 17 2219

HSV2-UL19-1980 + CGGCGACCAGGGUCGCC 17 2220

HSV2-UL19-1981 + GGCGACCAGGGUCGCCC 17 2221

HSV2-UL19-1982 + GGUCGCCCGGGCAACCC 17 2222

HSV2-UL19-1983 + GGGCAACCCUGGUCGCC 17 2223

HSV2-UL19-1984 + GUCGAGAUAUCGUUGCA 17 2224

HSV2-UL19-1985 + UCGAGAUAUCGUUGCAU 17 2225

HSV2-UL19-1986 + GUUGCAUGGGCAACAGC 17 2226

HSV2-UL19-1987 + UUGCAUGGGCAACAGCA 17 2227

HSV2-UL19-1988 + UGGGCAACAGCAGGGCC 17 2228

HSV2-UL19-1989 + G G G CAACAG CAG G G CCA 17 2229

HSV2-UL19-1990 + G G CAACAG CAG G G CCAG 17 2230

HSV2-UL19-1991 + AACAGCAGGGCCAGGGG 17 2231

HSV2-UL19-1992 + ACAGCACGUGCAGCAUC 17 2232

HSV2-UL19-1993 + CACGUGCAGCAUCUGGU 17 2233

HSV2-UL19-1994 + CGCGCUCAAACGCCCCC 17 2234

HSV2-UL19-1995 + CUCAAACG CCCCC AG G A 17 2235

HSV2-UL19-1996 + ACGCCCCCAGGACGGCC 17 2236

Table ID provides exemplary targeting domains for knocking out the UL19 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a strand targeting domain provided that the two gRNAs are oriented on the DNA such that

PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp

Table ID

HSV2-UL19-237 - GCGUGUGCACCAAGUUC 17 2265

HSV2-UL19-113 - CGUGUGCACCAAGUUCC 17 420

HSV2-UL19-239 - UCCCGGAGCUGGCUUAC 17 2266

HSV2-UL19-240 - GGAGCUGGCUUACAUGA 17 2267

HSV2-UL19-241 - GCUGGCUUACAUGAACG 17 2268

HSV2-UL19-242 - CGAAGGGCGUGUGCAGU 17 2269

HSV2-UL19-243 - CGACGGCCCGCACCCCG 17 2270

HSV2-UL19-244 - CCGUGCAUAAUUACAUG 17 2271

HSV2-UL19-245 - ACGAAGGUCAUCGACCG 17 2272

HSV2-UL19-246 - UCAUCGACCGCCGGGCC 17 2273

HSV2-UL19-247 - CGCCUUCAGCCUGGCCA 17 2274

HSV2-UL19-248 - AGGCCAUUGCCCUGCUC 17 2275

HSV2-UL19-124 - GGCCAUUGCCCUGCUCA 17 525

HSV2-UL19-125 - GCCAUUGCCCUGCUCAC 17 526

HSV2-UL19-126 - CCAUUGCCCUGCUCACG 17 527

HSV2-UL19-127 - CAUUGCCCUGCUCACGG 17 528

HSV2-UL19-253 - UGCUCACGGGGGAGGCC 17 2276

HSV2-UL19-254 - CACGGGGGAGGCCCUGG 17 2277

HSV2-UL19-130 - ACGGGGGAGGCCCUGGA 17 531

HSV2-UL19-256 - GGGAGGCCCUGGACGGG 17 2278

HSV2-UL19-257 + AGGCUAAUGCCCGUCCCGUC 20 2279

HSV2-UL19-258 + UCCCGUCCAGGGCCUCCCCC 20 2280

HSV2-UL19-259 + UCCAGGGCCUCCCCCGUGAG 20 2281

HSV2-UL19-260 + CAAUGGCCUCGGUGGCCAGG 20 2282

HSV2-UL19-261 + GCCAGGCUGAAGGCGGCGUU 20 2283

HSV2-UL19-262 + CCUUCGUCAUGUAAUUAUGC 20 2284

HSV2-UL19-263 + AAUUAUGCACGGGCUGCUCG 20 2285

HSV2-UL19-53 + AUUAUGCACGGGCUGCUCGA 20 401

HSV2-UL19-265 + CACGGGCUGCUCGACGGGGU 20 2286

HSV2-UL19-266 + UCGACGGGGUGCGGGCCGUC 20 2287

HSV2-UL19-267 + GGUGCGGGCCGUCGCGGGCG 20 2288

HSV2-UL19-268 + UGCGGGCCGUCGCGGGCGAU 20 2289

HSV2-UL19-60 + GCGGGCCGUCGCGGGCGAUG 20 483

HSV2-UL19-270 + UCGCGGGCGAUGAGGGGCUG 20 2290

HSV2-UL19-271 + CGAUGAGGGGCUGGUGGACC 20 2291

HSV2-UL19-272 + U U CG U U CAU G U AAG CCAGCU 20 2292

HSV2-UL19-65 + UCGUUCAUGUAAGCCAGCUC 20 405

HSV2-UL19-66 + CGUUCAUGUAAGCCAGCUCC 20 406

HSV2-UL19-275 + ACUUGGUGCACACGCACGCC 20 2293

HSV2-UL19-276 + ACACGCACGCCACGGACAGG 20 2294

HSV2-UL19-277 + GCCACGGACAGGCCGAGCUC 20 2295

HSV2-UL19-278 + GGCCGAGCUCCAGAAAGCGC 20 2296

HSV2-UL19-279 + UCCAGAAAGCGCACGAGCGA 20 2297

HSV2-UL19-280 + CGAGCGACAGGGUGUUGCAG 20 2298

HSV2-UL19-281 + GUGUUGCAGUAGGACCCCAG 20 2299

HSV2-UL19-282 + CGUCAUACAGGCUGUUUUCG 20 2300 HSV2-UL19-76 + GUCAUACAGGCUGUUUUCGU 20 494

HSV2-UL19-284 + UUUCGUCGGAGCGCACGGCG 20 2301

HSV2-UL19-285 + GCACGGCGGCG AAAAAAU CA 20 2302

HSV2-UL19-286 + AAAAAAU CAAAG AG U CU GCG 20 2303

HSV2-UL19-80 + AAAAAUCAAAGAGUCUGCGG 20 498

HSV2-UL19-288 + GACGCCACCUCGAUCGUACU 20 2304

HSV2-UL19-289 + CCACCUCGAUCGUACUCAGG 20 2305

HSV2-UL19-83 + CACCUCGAUCGUACUCAGGA 20 409

HSV2-UL19-291 + UCGUACUCAGGAUGGAGCCG 20 2306

HSV2-UL19-292 + CUCAGGAUGGAGCCGGUGGG 20 2307

HSV2-UL19-293 + GGCCGCGGCGUACCGGUAAC 20 2308

HSV2-UL19-91 + GCCGCGGCGUACCGGUAACC 20 506

HSV2-UL19-92 + CCGCGGCGUACCGGUAACCC 20 507

HSV2-UL19-93 + CGCGGCGUACCGGUAACCCG 20 508

HSV2-UL19-297 + UACCGGUAACCCGGGGGGUC 20 2309

HSV2-UL19-298 + GUAACCCGGGGGGUCGCGGG 20 2310

HSV2-UL19-98 + UAACCCGGGGGGUCGCGGGC 20 413

HSV2-UL19-300 + CUAAUGCCCGUCCCGUC 17 2311

HSV2-UL19-301 + CGUCCAGGGCCUCCCCC 17 2312

HSV2-UL19-302 + AGGGCCUCCCCCGUGAG 17 2313

HSV2-UL19-303 + UGGCCUCGGUGGCCAGG 17 2314

HSV2-UL19-304 + AGGCUGAAGGCGGCGUU 17 2315

HSV2-UL19-305 + UCGUCAUGUAAUUAUGC 17 2316

HSV2-UL19-306 + UAUGCACGGGCUGCUCG 17 2317

HSV2-UL19-152 + AUGCACGGGCUGCUCGA 17 428

HSV2-UL19-308 + GGGCUGCUCGACGGGGU 17 2318

HSV2-UL19-309 + ACGGGGUGCGGGCCGUC 17 2319

HSV2-UL19-310 + GCGGGCCGUCGCGGGCG 17 2320

HSV2-UL19-311 + GGGCCGUCGCGGGCGAU 17 2321

HSV2-UL19-159 + GGCCGUCGCGGGCGAUG 17 555

HSV2-UL19-313 + CGGGCGAUGAGGGGCUG 17 2322

HSV2-UL19-314 + UGAGGGGCUGGUGGACC 17 2323

HSV2-UL19-315 + GUUCAUGUAAGCCAGCU 17 2324

HSV2-UL19-164 + UUCAUGUAAGCCAGCUC 17 432

HSV2-UL19-165 + UCAUGUAAGCCAGCUCC 17 433

HSV2-UL19-318 + UGGUGCACACGCACGCC 17 2325

HSV2-UL19-319 + CGCACGCCACGGACAGG 17 2326

HSV2-UL19-320 + ACGGACAGGCCGAGCUC 17 2327

HSV2-UL19-321 + CGAGCUCCAGAAAGCGC 17 2328

HSV2-UL19-322 + AGAAAGCGCACGAGCGA 17 2329

HSV2-UL19-323 + GCGACAGGGUGUUGCAG 17 2330

HSV2-UL19-324 + U UGCAG U AG G ACCCCAG 17 2331

HSV2-UL19-325 + CAUACAGGCUGUUUUCG 17 2332

HSV2-UL19-175 + AUACAGGCUGUUUUCGU 17 566

HSV2-UL19-327 + CGUCGGAGCGCACGGCG 17 2333

HSV2-UL19-328 + CG G CG G CG AAAAAAU CA 17 2334 HSV2-UL19-329 + AAAUCAAAGAGUCUGCG 17 2335

HSV2-UL19-179 + AAU CAAAG AGUCUGCGG 17 570

HSV2-UL19-331 + GCCACCUCGAUCGUACU 17 2336

HSV2-UL19-332 + CCUCGAUCGUACUCAGG 17 2337

HSV2-UL19-182 + CUCGAUCGUACUCAGGA 17 436

HSV2-UL19-334 + UACUCAGGAUGGAGCCG 17 2338

HSV2-UL19-335 + AGGAUGGAGCCGGUGGG 17 2339

HSV2-UL19-336 + CGCGGCGUACCGGUAAC 17 2340

HSV2-UL19-190 + GCGGCGUACCGGUAACC 17 578

HSV2-UL19-191 + CGGCGUACCGGUAACCC 17 579

HSV2-UL19-192 + GGCGUACCGGUAACCCG 17 580

HSV2-UL19-340 + CGGUAACCCGGGGGGUC 17 2341

HSV2-UL19-341 + ACCCGGGGGGUCGCGGG 17 2342

HSV2-UL19-197 + CCCGGGGGGUCGCGGGC 17 440

Table IE provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of the UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table IE

HSV2-UL19-2005 - GGGCGACCCUGGUCGCCGAG 20 2350

HSV2-UL19-2006 - ACCCUGGUCGCCGAGCUGAA 20 2351

HSV2-UL19-359 - CCCUGGUCGCCGAGCUGAAG 20 599

HSV2-UL19-2008 - UGAAGCGGAGCUUUUGCGAC 20 2352

HSV2-UL19-2009 - UUUGCGACACGAGCUUCUUC 20 2353

HSV2-UL19-2010 - CGAGCUUCUUCCUGGGCAAG 20 2354

HSV2-UL19-2011 - GGGCAAGGCGGGCCAUCGCC 20 2355

HSV2-UL19-2012 - GGGCCAUCGCCGCGAGGCCA 20 2356

HSV2-UL19-2013 - AGGCCAUCGAGGCCUGGCUC 20 2357

HSV2-UL19-2014 - CCUGACGCACGCCGACACGC 20 2358

HSV2-UL19-2015 - CACGCGCGGGCGGCCGGUCG 20 2359

HSV2-UL19-375 - ACGCGCGGGCGGCCGGUCGA 20 615

HSV2-UL19-2017 - AGCGCCUCCUGCAGUCCUUC 20 2360

HSV2-UL19-2018 - UCCUGCAGUCCUUCCUGAAG 20 2361

HSV2-UL19-380 - CCUGCAGUCCUUCCUGAAGG 20 620

HSV2-UL19-2020 - UGCAGUCCUUCCUGAAGGUG 20 2362

HSV2-UL19-2021 - CUUCCUGAAGGUGGAGGACA 20 2363

HSV2-UL19-2022 - CGACGUGCCGGUGACCUACG 20 2364

HSV2-UL19-2023 - UGACCUACGGCGAGAUGGUC 20 2365

HSV2-UL19-2024 - CUACGGCGAGAUGGUCUUGA 20 2366

HSV2-UL19-386 - UACGGCGAGAUGGUCUUGAA 20 626

HSV2-UL19-2026 - ACCUCGUCACGGCGCUGGUG 20 2367

HSV2-UL19-2027 - CUGGUGAUGGGCAAGGCCGU 20 2368

HSV2-UL19-394 - UGGUGAUGGGCAAGGCCGUG 20 634

HSV2-UL19-2029 - UGGGCAAGGCCGUGCGGAGC 20 2369

HSV2-UL19-2030 - CCGUGCGGAGCCUGGACGAC 20 2370

HSV2-UL19-2031 - ACGACGUGGGCCGCCACCUG 20 2371

HSV2-UL19-2032 - GCCGCCACCUGCUGGAUAUG 20 2372

HSV2-UL19-399 - CCGCCACCUGCUGGAUAUGC 20 639

HSV2-UL19-2034 - GCCACCUGCUGGAUAUGCAG 20 2373

HSV2-UL19-400 - CCACCUGCUGGAUAUGCAGG 20 640

HSV2-UL19-2036 - GGAUAUGCAGGAGGAGCAAC 20 2374

HSV2-UL19-2037 - UGCAGGAGGAGCAACUCGAG 20 2375

HSV2-UL19-2038 - GAGGAGCAACUCGAGGCGAA 20 2376

HSV2-UL19-402 - AGGAGCAACUCGAGGCGAAC 20 642

HSV2-UL19-403 - GGAGCAACUCGAGGCGAACC 20 643

HSV2-UL19-2041 - UCGAGGCGAACCGGGAGACG 20 2377

HSV2-UL19-2042 - GGCGAACCGGGAGACGCUGG 20 2378

HSV2-UL19-2043 - CCGGGAGACGCUGGAUGAAC 20 2379

HSV2-UL19-2044 - CCCAGACAACGCGCGUGCGC 20 2380

HSV2-UL19-2045 - UAGGCGACAGGCUGGUCUUC 20 2381

HSV2-UL19-411 - AGGCGACAGGCUGGUCUUCC 20 651

HSV2-UL19-2047 - GGCUGGUCUUCCUGGAGGCC 20 2382

HSV2-UL19-413 - GCUGGUCUUCCUGGAGGCCC 20 653

HSV2-UL19-2049 - UGGUCUUCCUGGAGGCCCUG 20 2383

HSV2-UL19-2050 - CCAACGUGCCCUACCCCCUG 20 2384 HSV2-UL19-2051 - CCUACCCCCUGGUGGGCGCC 20 2385

HSV2-UL19-2052 - ACCUGACGUUCGUCCUGCCC 20 2386

HSV2-UL19-418 - CCUGACGUUCGUCCUGCCCC 20 658

HSV2-UL19-2054 - UGGGGCUGUUCAACCCGGCC 20 2387

HSV2-UL19-422 - GGGGCUGUUCAACCCGGCCA 20 662

HSV2-UL19-2056 - GGAGCGCUUCGCCGCGCACG 20 2388

HSV2-UL19-423 - GAGCGCUUCGCCGCGCACGC 20 663

HSV2-UL19-424 - AGCGCUUCGCCGCGCACGCC 20 664

HSV2-UL19-2059 - UGGUGCCCGCCCCCGGCCAC 20 2389

HSV2-UL19-428 - GGUGCCCGCCCCCGGCCACC 20 668

HSV2-UL19-2061 - CCUCCCCGGCAGCUGUUUUU 20 2390

HSV2-UL19-430 - CUCCCCGGCAGCUGUUUUUU 20 670

HSV2-UL19-431 - UCCCCGGCAGCUGUUUUUUU 20 671

HSV2-UL19-432 - CCCCGGCAGCUGU UUUUUUG 20 672

HSV2-UL19-2065 - GGCAGCUGUUUUUUUGGGGA 20 2391

HSV2-UL19-2066 - ACCAGGUUCUGCGGCUGUCC 20 2392

HSV2-UL19-436 - CCAGGUUCUGCGGCUGUCCA 20 676

HSV2-UL19-2068 - AGGUUCUGCGGCUGUCCAUG 20 2393

HSV2-UL19-2069 - GCUGUCCAUGGAGAACGCGG 20 2394

HSV2-UL19-438 - CUGUCCAUGGAGAACGCGGU 20 678

HSV2-UL19-2071 - CCGUGUGUCAUCCUUCGCUC 20 2395

HSV2-UL19-2072 - CAUGAACAUCGACGCGGCCG 20 2396

HSV2-UL19-441 - AUGAACAUCGACGCGGCCGU 20 681

HSV2-UL19-442 - UGAACAUCGACGCGGCCGUC 20 682

HSV2-UL19-2075 - UCGACGCGGCCGUCGGGGGC 20 2397

HSV2-UL19-2076 - GGGCGUGAACCACGACCCCG 20 2398

HSV2-UL19-2077 - ACCACGACCCCGUCGAGGCC 20 2399

HSV2-UL19-2078 - CGUCGAGGCCGCGAAUCCGU 20 2400

HSV2-UL19-446 - GUCGAGGCCGCGAAUCCGUA 20 686

HSV2-UL19-2080 - CGGCCCCGGCCGGCCCCGGC 20 2401

HSV2-UL19-2081 - CGGACAUGCAGCAGCGUUUU 20 2402

HSV2-UL19-2082 - CGGCAGCGCCUCGCCCACGG 20 2403

HSV2-UL19-2083 - CUCGCCCACGGCCGGGUCCG 20 2404

HSV2-UL19-2084 - CGGCCGGGUCCGGUGGGUCG 20 2405

HSV2-UL19-2085 - UCGCCGAGUGCCAGAUGACC 20 2406

HSV2-UL19-462 - CGCCGAGUGCCAGAUGACCG 20 702

HSV2-UL19-2087 - CCGACAACGCCAACCUGGCU 20 2407

HSV2-UL19-464 - CGACAACGCCAACCUGGCUC 20 704

HSV2-UL19-2089 - ACCCCGCGUUCGACUUCUUC 20 2408

HSV2-UL19-2090 - CUUCGCGGGCGUGGCCGACG 20 2409

HSV2-UL19-2091 - CGACGUCGAGCUUCCCGGCG 20 2410

HSV2-UL19-2092 - GCGAAGUCCCCCCGGCCGGU 20 2411

HSV2-UL19-472 - CGAAGUCCCCCCGGCCGGUC 20 712

HSV2-UL19-473 - GAAG U CCCCCCG GCCG G U CC 20 713

HSV2-UL19-2095 - CCGGUGGCGUUUCGUGACGC 20 2412

HSV2-UL19-483 - CGGUGGCGUUUCGUGACGCC 20 723 HSV2-UL19-2097 - CGUUUCGUGACGCCCGGGGC 20 2413

HSV2-UL19-486 - GUUUCGUGACGCCCGGGGCC 20 726

HSV2-UL19-2099 - GGCUACCAUAGCCGCCGUCC 20 2414

HSV2-UL19-491 - GCUACCAUAGCCGCCGUCCG 20 731

HSV2-UL19-2101 - AGCCGCCGUCCGCGGGGCGU 20 2415

HSV2-UL19-2102 - CCGCCGUCCGCGGGGCGUUC 20 2416

HSV2-UL19-2103 - GCAAGCCGCGAUUCACGGCA 20 2417

HSV2-UL19-2104 - ACUCAGUGCAUCACCAGCUA 20 2418

HSV2-UL19-500 - CUCAGUGCAUCACCAGCUAC 20 740

HSV2-UL19-2106 - ACACGCGAUGCGCGGCGUUC 20 2419

HSV2-UL19-2107 - CUCGUACAUCGUGACCUACC 20 2420

HSV2-UL19-503 - UCGUACAUCGUGACCUACCU 20 743

HSV2-UL19-504 - CGUACAUCGUGACCUACCUC 20 744

HSV2-UL19-2110 - CUACCUCGGGGGCGACCUCC 20 2421

HSV2-UL19-2111 - ACCUCGGGGGCGACCUCCCC 20 2422

HSV2-UL19-507 - CCUCGGGGGCGACCUCCCCG 20 747

HSV2-UL19-2113 - GAGGAGUGCAUGGCCGUGUA 20 2423

HSV2-UL19-509 - AGGAGUGCAUGGCCGUGUAU 20 749

HSV2-UL19-2115 - UCGGGACCUGGUGGCCCACG 20 2424

HSV2-UL19-2116 - UCGAGGCCCUGGCCCAGCUG 20 2425

HSV2-UL19-2117 - UGGUGGACGACUUUACCCUG 20 2426

HSV2-UL19-2118 - ACGACUUUACCCUGCCGGGC 20 2427

HSV2-UL19-519 - CGACUUUACCCUGCCGGGCC 20 759

HSV2-UL19-2120 - UUACCCUGCCGGGCCCGGAG 20 2428

HSV2-UL19-2121 - CCUGCCGGGCCCGGAGCUGG 20 2429

HSV2-UL19-2122 - GCUGGGCGGGCAGGCUCAGG 20 2430

HSV2-UL19-2123 - GCGGGCAGGCUCAGGCCGAG 20 2431

HSV2-UL19-2124 - GCGCUGCUGCCGCCCCUCGU 20 2432

HSV2-UL19-527 - CGCUGCUGCCGCCCCUCGUG 20 767

HSV2-UL19-2126 - GCCUUAUGCGACACGCGGCC 20 2433

HSV2-UL19-2127 - CACGCGGCCCUGGACCGCCA 20 2434

HSV2-UL19-2128 - CUGGACCGCCACCGAGACUG 20 2435

HSV2-UL19-2129 - ACCGAGACUGCCGGAUUGAC 20 2436

HSV2-UL19-533 - CCGAGACUGCCGGAUUGACG 20 773

HSV2-UL19-534 - CGAGACUGCCGGAUUGACGC 20 774

HSV2-UL19-535 - GAGACUGCCGGAUUGACGCG 20 775

HSV2-UL19-536 - AGACUGCCGGAUUGACGCGG 20 776

HSV2-UL19-2134 - CCGGAUUGACGCGGGGGGGC 20 2437

HSV2-UL19-2135 - CGGCCGCACCGGCCGGCCGA 20 2438

HSV2-UL19-2136 - GUGCUGGUGCCGGCCUUCUC 20 2439

HSV2-UL19-553 - UGCUGGUGCCGGCCUUCUCG 20 793

HSV2-UL19-554 - GCUGGUGCCGGCCUUCUCGC 20 794

HSV2-UL19-2139 - CGCGGGGGCGCUGCUGCACC 20 2440

HSV2-UL19-557 - GCGGGGGCGCUGCUGCACCG 20 797

HSV2-UL19-558 - CGGGGGCGCUGCUGCACCGC 20 798

HSV2-UL19-2142 - ACCGCGUGUACGCCACGCUG 20 2441 HSV2-UL19-2143 - CGCUGCAGAACAUGGUGGUC 20 2442

HSV2-UL19-563 - GCUGCAGAACAUGGUGGUCC 20 803

HSV2-UL19-2145 - GGUCCCGGAGAUCGCCCCCG 20 2443

HSV2-UL19-2146 - UCCCGGAGAUCGCCCCCGGU 20 2444

HSV2-UL19-565 - CCCGGAGAUCGCCCCCGGUG 20 805

HSV2-UL19-2148 - UCGCCCCCGGUGAGGAGUGC 20 2445

HSV2-UL19-2149 - GGUCAAGCGCAUGUUCCACA 20 2446

HSV2-UL19-2150 - CAACGGGCGCGUCGUCGUCG 20 2447

HSV2-UL19-2151 - GGUGCUGGCGCACAACAUGG 20 2448

HSV2-UL19-2152 - UGUGCUCCGCGGCGCCCGAC 20 2449

HSV2-UL19-2153 - GGCCAACAUGCGCAUCUUCG 20 2450

HSV2-UL19-581 - GCCAACAUGCGCAUCUUCGA 20 821

HSV2-UL19-2155 - UGCUCAUGGCCCCCCAGCAC 20 2451

HSV2-UL19-2156 - G G ACCACACCAU CCAAAAUG 20 2452

HSV2-UL19-2157 - UGCCCGUCCACGCGCUGUUU 20 2453

HSV2-UL19-2158 - UCCCCCUGGUCCCCCCGGCC 20 2454

HSV2-UL19-595 - CCCCCUGGUCCCCCCGGCCC 20 835

HSV2-UL19-596 - CCCCUGGUCCCCCCGGCCCU 20 836

HSV2-UL19-2161 - GCCCGUGGUGCAGCACGCCC 20 2455

HSV2-UL19-2162 - CCGUGGUGCAGCACGCCCGC 20 2456

HSV2-UL19-2163 - AGCACGCCCGCGAGAGCGCG 20 2457

HSV2-UL19-601 - GCACGCCCGCGAGAGCGCGG 20 841

HSV2-UL19-602 - CACGCCCGCGAGAGCGCGGC 20 842

HSV2-UL19-603 - ACGCCCGCGAGAGCGCGGCG 20 843

HSV2-UL19-604 - CGCCCGCGAGAGCGCGGCGG 20 844

HSV2-UL19-2168 - CCCGCGAGAGCGCGGCGGGG 20 2458

HSV2-UL19-2169 - CGCUGACCUACGCGCUCAUG 20 2459

HSV2-UL19-606 - GCUGACCUACGCGCUCAUGG 20 846

HSV2-UL19-2171 - UCAUGGCGGGGUACUUCAAG 20 2460

HSV2-UL19-2172 - CCCUGUAUCACCAGCUCAAG 20 2461

HSV2-UL19-2173 - GCUCAAGACGGGCCUCCACC 20 2462

HSV2-UL19-2174 - GACGGGCCUCCACCCCGGGU 20 2463

HSV2-UL19-2175 - UCGGGUUCACCGUCGUGCGG 20 2464

HSV2-UL19-2176 - GCGGCAGGACCGCUUCGUGA 20 2465

HSV2-UL19-2177 - GGCAGGACCGCUUCGUGACC 20 2466

HSV2-UL19-2178 - GACCGAGAACGUGCUGUUUU 20 2467

HSV2-UL19-2179 - UGCUGUUUUCCGAGCGCGCG 20 2468

HSV2-UL19-618 - GCUGUUUUCCGAGCGCGCGU 20 858

HSV2-UL19-2181 - GCGCGUCGGAGGCGUACUUU 20 2469

HSV2-UL19-2182 - CCAGCUCCAGGUGGCCCGCC 20 2470

HSV2-UL19-2183 - UCCAGGUGGCCCGCCACGAA 20 2471

HSV2-UL19-2184 - GGUGGCCCGCCACGAAACGG 20 2472

HSV2-UL19-626 - GUGGCCCGCCACGAAACGGG 20 866

HSV2-UL19-627 - UGGCCCGCCACGAAACGGGC 20 867

HSV2-UL19-628 - GGCCCGCCACGAAACGGGCG 20 868

HSV2-UL19-629 - GCCCGCCACGAAACGGGCGG 20 869 HSV2-UL19-2189 - CUUCACGCUCACCCAGCCGC 20 2473

HSV2-UL19-632 - UUCACGCUCACCCAGCCGCG 20 872

HSV2-UL19-2191 - UCACCCAGCCGCGCGGAAAC 20 2474

HSV2-UL19-2192 - AGCCGCGCGGAAACGUGGAC 20 2475

HSV2-UL19-2193 - GCGGAAACGUGGACCUGGGU 20 2476

HSV2-UL19-2194 - GCUACACCGCCGUCGCGGCC 20 2477

HSV2-UL19-2195 - GCACCGUCCGCAACCCCGUU 20 2478

HSV2-UL19-2196 - UCCGCAACCCCGUUACGGAC 20 2479

HSV2-UL19-2197 - CCAAAACUUUUACCUCGGCC 20 2480

HSV2-UL19-645 - CAAAACUUUUACCUCGGCCG 20 885

HSV2-UL19-2199 - ACCUGCGCAACGCGGUCGUG 20 2481

HSV2-UL19-651 - CCUGCGCAACGCGGUCGUGG 20 891

HSV2-UL19-652 - CUGCGCAACGCGGUCGUGGC 20 892

HSV2-UL19-2202 - CGGUCGUGGCGGGAAACCGG 20 2482

HSV2-UL19-654 - GGUCGUGGCGGGAAACCGGC 20 894

HSV2-UL19-2204 - AGGUGCCGCGGCGCGCCGGC 20 2483

HSV2-UL19-2205 - GCGGCGCGCCGGCAUGGACC 20 2484

HSV2-UL19-2206 - GCGCCGGCAUGGACCACGGG 20 2485

HSV2-UL19-2207 - CCACGGGCAGGAUGCCGUGU 20 2486

HSV2-UL19-2208 - UCAUCGCCACCCCCGUGGCC 20 2487

HSV2-UL19-2209 - UUUCGCCGGCCCUGCAACCC 20 2488

HSV2-UL19-670 - UUCGCCGGCCCUGCAACCCG 20 910

HSV2-UL19-671 - UCGCCGGCCCUGCAACCCGC 20 911

HSV2-UL19-2212 - GCGCGGCCGGCGGCGUGUAC 20 2489

HSV2-UL19-676 - CGCGGCCGGCGGCGUGUACG 20 916

HSV2-UL19-677 - GCGGCCGGCGGCGUGUACGC 20 917

HSV2-UL19-678 - CGGCCGGCGGCGUGUACGCG 20 918

HSV2-UL19-2216 - GCGGCGUGUACGCGGGGGAC 20 2490

HSV2-UL19-680 - CGGCGUGUACGCGGGGGACA 20 920

HSV2-UL19-2218 - GCGUGUACGCGGGGGACAAG 20 2491

HSV2-UL19-681 - CGUGUACGCGGGGGACAAGG 20 921

HSV2-UL19-682 - GUGUACGCGGGGGACAAGGA 20 922

HSV2-UL19-683 - UGUACGCGGGGGACAAGGAG 20 923

HSV2-UL19-2222 - CCCUCAUGUACGACCACGGC 20 2492

HSV2-UL19-2223 - UUCGCGGCCACGGCCAACCC 20 2493

HSV2-UL19-2224 - GGCGUCGCAGCGGUUCUCGU 20 2494

HSV2-UL19-693 - GCGUCGCAGCGGUUCUCGUA 20 933

HSV2-UL19-694 - CGUCGCAGCGGUUCUCGUAC 20 934

HSV2-UL19-2227 - GUACGGGGACCUGCUGUACA 20 2495

HSV2-UL19-696 - UACGGGGACCUGCUGUACAA 20 936

HSV2-UL19-2229 - CAACGGGGCCUAUCACCUCA 20 2496

HSV2-UL19-699 - AACGGGGCCUAUCACCUCAA 20 939

HSV2-UL19-2231 - UCACGGCCAAACAUCGCUGC 20 2497

HSV2-UL19-704 - CACGGCCAAACAUCGCUGCC 20 944

HSV2-UL19-2233 - GCUGCCUGGAGCGUCUCAUC 20 2498

HSV2-UL19-705 - CUGCCUGGAGCGUCUCAUCG 20 945 HSV2-UL19-2235 - UGGAGCGUCUCAUCGUGGAA 20 2499

HSV2-UL19-706 - GGAGCGUCUCAUCGUGGAAA 20 946

HSV2-UL19-2237 - ACGUGCAGUUUAAGCGCCCG 20 2500

HSV2-UL19-711 - CGUGCAGUUUAAGCGCCCGC 20 951

HSV2-UL19-2239 - UAAGCGCCCGCCGGGGUGCC 20 2501

HSV2-UL19-2240 - CGCCGGGGUGCCGCGAGCUC 20 2502

HSV2-UL19-714 - GCCGGGGUGCCGCGAGCUCG 20 954

HSV2-UL19-2242 - AAGACCCGUGCGGCCUGUUU 20 2503

HSV2-UL19-716 - AGACCCGUGCGGCCUGUUUC 20 956

HSV2-UL19-2244 - CCUGCUACGCAGCGCCCGCG 20 2504

HSV2-UL19-717 - CUGCUACGCAGCGCCCGCGA 20 957

HSV2-UL19-718 - UGCUACGCAGCGCCCGCGAU 20 958

HSV2-UL19-719 - GCUACGCAGCGCCCGCGAUG 20 959

HSV2-UL19-2248 - CGCGAUGGGGAGGCCCACGC 20 2505

HSV2-UL19-2249 - CGAUGGGGAGGCCCACGCGC 20 2506

HSV2-UL19-2250 - UCUACGACGCCUCCCCGCUA 20 2507

HSV2-UL19-2251 - GGCCGUCCUGGGGGCGU 17 2508

HSV2-UL19-2252 - AGAUGCUGCACGUGCUG 17 2509

HSV2-UL19-726 - GAUGCUGCACGUGCUGU 17 966

HSV2-UL19-2254 - UGCUGCACGUGCUGUUG 17 2510

HSV2-UL19-2255 - GCAACGAUAUCUCGACA 17 2511

HSV2-UL19-2256 - AACGGGCGCCUGGCGAC 17 2512

HSV2-UL19-2257 - CUGGCGACCAGGGUUGC 17 2513

HSV2-UL19-2258 - CCGGGCGACCCUGGUCG 17 2514

HSV2-UL19-2259 - CGACCCUGGUCGCCGAG 17 2515

HSV2-UL19-2260 - CUGGUCGCCGAGCUGAA 17 2516

HSV2-UL19-737 - UGGUCGCCGAGCUGAAG 17 977

HSV2-UL19-2262 - AGCGGAGCUUUUGCGAC 17 2517

HSV2-UL19-2263 - GCGACACGAGCUUCUUC 17 2518

HSV2-UL19-2264 - GCU UCUUCCUGGGCAAG 17 2519

HSV2-UL19-2265 - CAAG GCGGGCCAUCGCC 17 2520

HSV2-UL19-2266 - CCAUCGCCGCGAGGCCA 17 2521

HSV2-UL19-2267 - CCAUCGAGGCCUGGCUC 17 2522

HSV2-UL19-2268 - GACGCACGCCGACACGC 17 2523

HSV2-UL19-2269 - GCGCGGGCGGCCGGUCG 17 2524

HSV2-UL19-753 - CGCGGGCGGCCGGUCGA 17 993

HSV2-UL19-2271 - GCCUCCUGCAGUCCUUC 17 2525

HSV2-UL19-2272 - UGCAGUCCUUCCUGAAG 17 2526

HSV2-UL19-758 - GCAGUCCUUCCUGAAGG 17 998

HSV2-UL19-2274 - AGUCCUUCCUGAAGGUG 17 2527

HSV2-UL19-2275 - CCUGAAGGUGGAGGACA 17 2528

HSV2-UL19-2276 - CGUGCCGGUGACCUACG 17 2529

HSV2-UL19-2277 - CCUACGGCGAGAUGGUC 17 2530

HSV2-UL19-2278 - CGGCGAGAUGGUCUUGA 17 2531

HSV2-UL19-764 - GGCGAGAUGGUCUUGAA 17 1004

HSV2-UL19-2280 - UCGUCACGGCGCUGGUG 17 2532 HSV2-UL19-2281 - GUGAUGGGCAAGGCCGU 17 2533

HSV2-UL19-772 - UGAUGGGCAAGGCCGUG 17 1012

HSV2-UL19-2283 - GCAAGGCCGUGCGGAGC 17 2534

HSV2-UL19-2284 - UGCGGAGCCUGGACGAC 17 2535

HSV2-UL19-2285 - ACGUGGGCCGCCACCUG 17 2536

HSV2-UL19-2286 - GCCACCUGCUGGAUAUG 17 2537

HSV2-UL19-777 - CCACCUGCUGGAUAUGC 17 1017

HSV2-UL19-2288 - ACCUGCUGGAUAUGCAG 17 2538

HSV2-UL19-778 - CCUGCUGGAUAUGCAGG 17 1018

HSV2-UL19-2290 - UAUGCAGGAGGAGCAAC 17 2539

HSV2-UL19-2291 - AGGAGGAGCAACUCGAG 17 2540

HSV2-UL19-2292 - GAGCAACUCGAGGCGAA 17 2541

HSV2-UL19-780 - AGCAACUCGAGGCGAAC 17 1020

HSV2-UL19-781 - GCAACUCGAGGCGAACC 17 1021

HSV2-UL19-2295 - AGGCGAACCGGGAGACG 17 2542

HSV2-UL19-2296 - GAACCGGGAGACGCUGG 17 2543

HSV2-UL19-2297 - GGAGACGCUGGAUGAAC 17 2544

HSV2-UL19-2298 - AGACAACGCGCGUGCGC 17 2545

HSV2-UL19-2299 - GCGACAGGCUGGUCUUC 17 2546

HSV2-UL19-789 - CGACAGGCUGGUCUUCC 17 1029

HSV2-UL19-2301 - UGGUCUUCCUGGAGGCC 17 2547

HSV2-UL19-791 - GGUCUUCCUGGAGGCCC 17 1031

HSV2-UL19-2303 - UCUUCCUGGAGGCCCUG 17 2548

HSV2-UL19-2304 - ACGUGCCCUACCCCCUG 17 2549

HSV2-UL19-2305 - ACCCCCUGGUGGGCGCC 17 2550

HSV2-UL19-2306 - UGACGUUCGUCCUGCCC 17 2551

HSV2-UL19-796 - GACGUUCGUCCUGCCCC 17 1036

HSV2-UL19-2308 - GGCUGUUCAACCCGGCC 17 2552

HSV2-UL19-800 - GCUGUUCAACCCGGCCA 17 1040

HSV2-UL19-2310 - GCGCUUCGCCGCGCACG 17 2553

HSV2-UL19-801 - CGCU UCGCCGCGCACGC 17 1041

HSV2-UL19-802 - GCU UCGCCGCGCACGCC 17 1042

HSV2-UL19-2313 - UGCCCGCCCCCGGCCAC 17 2554

HSV2-UL19-806 - GCCCGCCCCCGGCCACC 17 1046

HSV2-UL19-2315 - CCCCGGCAGCUGUUUUU 17 2555

HSV2-UL19-808 - CCCGGCAGCUGUUUUUU 17 1048

HSV2-UL19-809 - CCGGCAGCUGUUUUUUU 17 1049

HSV2-UL19-810 - CGGCAGCUGUUUUUUUG 17 1050

HSV2-UL19-2319 - AGCUGUUUUUUUGGGGA 17 2556

HSV2-UL19-2320 - AGGUUCUGCGGCUGUCC 17 2557

HSV2-UL19-814 - GGUUCUGCGGCUGUCCA 17 1054

HSV2-UL19-2322 - UUCUGCGGCUGUCCAUG 17 2558

HSV2-UL19-2323 - G U CCAUG G AG AACG CG G 17 2559

HSV2-UL19-816 - UCCAUGGAGAACGCGGU 17 1056

HSV2-UL19-2325 - UGUGUCAUCCUUCGCUC 17 2560

HSV2-UL19-2326 - GAACAUCGACGCGGCCG 17 2561 HSV2-UL19-819 - AACAUCGACGCGGCCGU 17 1059

HSV2-UL19-820 - ACAUCGACGCGGCCGUC 17 1060

HSV2-UL19-2329 - ACGCGGCCGUCGGGGGC 17 2562

HSV2-UL19-2330 - CG U G AACCACG ACCCCG 17 2563

HSV2-UL19-2331 - ACGACCCCGUCGAGGCC 17 2564

HSV2-UL19-2332 - CGAGGCCGCGAAUCCGU 17 2565

HSV2-UL19-824 - GAGGCCGCGAAUCCGUA 17 1064

HSV2-UL19-2334 - CCCCGGCCGGCCCCGGC 17 2566

HSV2-UL19-2335 - ACAUGCAGCAGCGUUUU 17 2567

HSV2-UL19-2336 - CAGCGCCUCGCCCACGG 17 2568

HSV2-UL19-2337 - GCCCACGGCCGGGUCCG 17 2569

HSV2-UL19-2338 - CCGGGUCCGGUGGGUCG 17 2570

HSV2-UL19-2339 - CCGAGUGCCAGAUGACC 17 2571

HSV2-UL19-840 - CGAGUGCCAGAUGACCG 17 1080

HSV2-UL19-2341 - ACAACGCCAACCUGGCU 17 2572

HSV2-UL19-842 - CAACGCCAACCUGGCUC 17 1082

HSV2-UL19-2343 - CCGCGUUCGACUUCUUC 17 2573

HSV2-UL19-2344 - CGCGGGCGUGGCCGACG 17 2574

HSV2-UL19-2345 - CGUCGAGCUUCCCGGCG 17 2575

HSV2-UL19-2346 - AAGUCCCCCCGGCCGGU 17 2576

HSV2-UL19-850 - AGUCCCCCCGGCCGGUC 17 1090

HSV2-UL19-851 - GUCCCCCCGGCCGGUCC 17 1091

HSV2-UL19-2349 - GUGGCGUUUCGUGACGC 17 2577

HSV2-UL19-861 - UGGCGUUUCGUGACGCC 17 1101

HSV2-UL19-2351 - UUCGUGACGCCCGGGGC 17 2578

HSV2-UL19-864 - UCGUGACGCCCGGGGCC 17 1104

HSV2-UL19-2353 - UACCAUAGCCGCCGUCC 17 2579

HSV2-UL19-869 - ACCAUAGCCGCCGUCCG 17 1109

HSV2-UL19-2355 - CGCCGUCCGCGGGGCGU 17 2580

HSV2-UL19-2356 - CCGUCCGCGGGGCGUUC 17 2581

HSV2-UL19-2357 - AGCCGCGAUUCACGGCA 17 2582

HSV2-UL19-2358 - CAGUGCAUCACCAGCUA 17 2583

HSV2-UL19-878 - AGUGCAUCACCAGCUAC 17 1118

HSV2-UL19-2360 - CGCGAUGCGCGGCGUUC 17 2584

HSV2-UL19-2361 - GUACAUCGUGACCUACC 17 2585

HSV2-UL19-881 - UACAUCGUGACCUACCU 17 1121

HSV2-UL19-882 - ACAUCGUGACCUACCUC 17 1122

HSV2-UL19-2364 - CCUCGGGGGCGACCUCC 17 2586

HSV2-UL19-2365 - UCGGGGGCGACCUCCCC 17 2587

HSV2-UL19-885 - CGGGGGCGACCUCCCCG 17 1125

HSV2-UL19-2367 - GAGUGCAUGGCCGUGUA 17 2588

HSV2-UL19-887 - AGUGCAUGGCCGUGUAU 17 1127

HSV2-UL19-2369 - GGACCUGGUGGCCCACG 17 2589

HSV2-UL19-2370 - AGGCCCUGGCCCAGCUG 17 2590

HSV2-UL19-2371 - UGGACGACUUUACCCUG 17 2591

HSV2-UL19-2372 - ACUUUACCCUGCCGGGC 17 2592 HSV2-UL19-897 - CUUUACCCUGCCGGGCC 17 1137

HSV2-UL19-2374 - CCCUGCCGGGCCCGGAG 17 2593

HSV2-UL19-2375 - GCCGGGCCCGGAGCUGG 17 2594

HSV2-UL19-2376 - GGGCGGGCAGGCUCAGG 17 2595

HSV2-UL19-2377 - GGCAGGCUCAGGCCGAG 17 2596

HSV2-UL19-2378 - CUGCUGCCGCCCCUCGU 17 2597

HSV2-UL19-905 - UGCUGCCGCCCCUCGUG 17 1145

HSV2-UL19-2380 - UUAUGCGACACGCGGCC 17 2598

HSV2-UL19-2381 - GCGGCCCUGGACCGCCA 17 2599

HSV2-UL19-2382 - GACCGCCACCGAGACUG 17 2600

HSV2-UL19-2383 - GAGACUGCCGGAUUGAC 17 2601

HSV2-UL19-911 - AGACUGCCGGAUUGACG 17 1151

HSV2-UL19-912 - GACUGCCGGAUUGACGC 17 1152

HSV2-UL19-913 - ACUGCCGGAUUGACGCG 17 1153

HSV2-UL19-914 - CUGCCGGAUUGACGCGG 17 1154

HSV2-UL19-2388 - GAUUGACGCGGGGGGGC 17 2602

HSV2-UL19-2389 - CCGCACCGGCCGGCCGA 17 2603

HSV2-UL19-2390 - CUGGUGCCGGCCUUCUC 17 2604

HSV2-UL19-931 - UGGUGCCGGCCUUCUCG 17 1171

HSV2-UL19-932 - GGUGCCGGCCUUCUCGC 17 1172

HSV2-UL19-2393 - GGGGGCGCUGCUGCACC 17 2605

HSV2-UL19-935 - GGGGCGCUGCUGCACCG 17 1175

HSV2-UL19-936 - GGGCGCUGCUGCACCGC 17 1176

HSV2-UL19-2396 - GCGUGUACGCCACGCUG 17 2606

HSV2-UL19-2397 - UGCAGAACAUGGUGGUC 17 2607

HSV2-UL19-941 - GCAGAACAUGGUGGUCC 17 1181

HSV2-UL19-2399 - CCCGGAGAUCGCCCCCG 17 2608

HSV2-UL19-2400 - CGGAGAUCGCCCCCGGU 17 2609

HSV2-UL19-943 - GGAGAUCGCCCCCGGUG 17 1183

HSV2-UL19-2402 - CCCCCGGUGAGGAGUGC 17 2610

HSV2-UL19-2403 - CAAGCGCAUGUUCCACA 17 2611

HSV2-UL19-2404 - CGGGCGCGUCGUCGUCG 17 2612

HSV2-UL19-2405 - GCUGGCGCACAACAUGG 17 2613

HSV2-UL19-2406 - GCUCCGCGGCGCCCGAC 17 2614

HSV2-UL19-2407 - CAACAU GCG CAU CU U CG 17 2615

HSV2-UL19-959 - AACAUGCGCAUCUUCGA 17 1199

HSV2-UL19-2409 - UCAUGGCCCCCCAGCAC 17 2616

HSV2-UL19-2410 - CCACACCAU CCAAAAU G 17 2617

HSV2-UL19-2411 - CCGUCCACGCGCUGUUU 17 2618

HSV2-UL19-2412 - CCCUGGUCCCCCCGGCC 17 2619

HSV2-UL19-973 - CCUGGUCCCCCCGGCCC 17 1213

HSV2-UL19-974 - CUGGUCCCCCCGGCCCU 17 1214

HSV2-UL19-2415 - CGUGGUGCAGCACGCCC 17 2620

HSV2-UL19-2416 - UGGUGCAGCACGCCCGC 17 2621

HSV2-UL19-2417 - ACGCCCGCGAGAGCGCG 17 2622

HSV2-UL19-979 - CGCCCGCGAGAGCGCGG 17 1219 HSV2-UL19-980 - GCCCGCGAGAGCGCGGC 17 1220

HSV2-UL19-981 - CCCGCGAGAGCGCGGCG 17 1221

HSV2-UL19-982 - CCGCGAGAGCGCGGCGG 17 1222

HSV2-UL19-2422 - GCGAGAGCGCGGCGGGG 17 2623

HSV2-UL19-2423 - UGACCUACGCGCUCAUG 17 2624

HSV2-UL19-984 - GACCUACGCGCUCAUGG 17 1224

HSV2-UL19-2425 - UGGCGGGGUACUUCAAG 17 2625

HSV2-UL19-2426 - UGUAUCACCAGCUCAAG 17 2626

HSV2-UL19-2427 - CAAGACGGGCCUCCACC 17 2627

HSV2-UL19-2428 - GGGCCUCCACCCCGGGU 17 2628

HSV2-UL19-2429 - GGUUCACCGUCGUGCGG 17 2629

HSV2-UL19-2430 - GCAGGACCGCUUCGUGA 17 2630

HSV2-UL19-2431 - AGGACCGCUUCGUGACC 17 2631

HSV2-UL19-2432 - CGAGAACGUGCUGUUUU 17 2632

HSV2-UL19-2433 - UGUUUUCCGAGCGCGCG 17 2633

HSV2-UL19-996 - GUUUUCCGAGCGCGCGU 17 1236

HSV2-UL19-2435 - CGUCGGAGGCGUACUUU 17 2634

HSV2-UL19-2436 - GCUCCAGGUGGCCCGCC 17 2635

HSV2-UL19-2437 - AGGUGGCCCGCCACGAA 17 2636

HSV2-UL19-2438 - GGCCCGCCACGAAACGG 17 2637

HSV2-UL19-1004 - GCCCGCCACGAAACGGG 17 1244

HSV2-UL19-1005 - CCCGCCACGAAACGGGC 17 1245

HSV2-UL19-1006 - CCGCCACGAAACGGGCG 17 1246

HSV2-UL19-1007 - CGCCACGAAACGGGCGG 17 1247

HSV2-UL19-2443 - CACGCUCACCCAGCCGC 17 2638

HSV2-UL19-1010 - ACG CU CACCCAGCCG CG 17 1250

HSV2-UL19-2445 - CCCAGCCGCGCGGAAAC 17 2639

HSV2-UL19-2446 - CGCGCGGAAACGUGGAC 17 2640

HSV2-UL19-2447 - GAAACGUGGACCUGGGU 17 2641

HSV2-UL19-2448 - ACACCGCCGUCGCGGCC 17 2642

HSV2-UL19-2449 - CCG U CCG CAACCCCG U U 17 2643

HSV2-UL19-2450 - GCAACCCCGU UACGGAC 17 2644

HSV2-UL19-2451 - AAACU U U UACCUCGGCC 17 2645

HSV2-UL19-1023 - AACUUUUACCUCGGCCG 17 1263

HSV2-UL19-2453 - UGCGCAACGCGGUCGUG 17 2646

HSV2-UL19-1029 - GCGCAACGCGGUCGUGG 17 1269

HSV2-UL19-1030 - CGCAACGCGGUCGUGGC 17 1270

HSV2-UL19-2456 - UCGUGGCGGGAAACCGG 17 2647

HSV2-UL19-1032 - CGUGGCGGGAAACCGGC 17 1272

HSV2-UL19-2458 - UGCCGCGGCGCGCCGGC 17 2648

HSV2-UL19-2459 - GCGCGCCGGCAUGGACC 17 2649

HSV2-UL19-2460 - CCGGCAUGGACCACGGG 17 2650

HSV2-UL19-2461 - CGGGCAGGAUGCCGUGU 17 2651

HSV2-UL19-2462 - UCGCCACCCCCGUGGCC 17 2652

HSV2-UL19-2463 - CGCCGGCCCUGCAACCC 17 2653

HSV2-UL19-1048 - GCCGGCCCUGCAACCCG 17 1288 HSV2-UL19-1049 - CCGGCCCUGCAACCCGC 17 1289

HSV2-UL19-2466 - CGGCCGGCGGCGUGUAC 17 2654

HSV2-UL19-1054 - GGCCGGCGGCGUGUACG 17 1294

HSV2-UL19-1055 - GCCGGCGGCGUGUACGC 17 1295

HSV2-UL19-1056 - CCGGCGGCGUGUACGCG 17 1296

HSV2-UL19-2470 - GCGUGUACGCGGGGGAC 17 2655

HSV2-UL19-1058 - CGUGUACGCGGGGGACA 17 1298

HSV2-UL19-2472 - UGUACGCGGGGGACAAG 17 2656

HSV2-UL19-1059 - GUACGCGGGGGACAAGG 17 1299

HSV2-UL19-1060 - UACGCGGGGGACAAGGA 17 1300

HSV2-UL19-1061 - ACGCGGGGGACAAGGAG 17 1301

HSV2-UL19-2476 - UCAUGUACGACCACGGC 17 2657

HSV2-UL19-2477 - GCGGCCACGGCCAACCC 17 2658

HSV2-UL19-2478 - GUCGCAGCGGUUCUCGU 17 2659

HSV2-UL19-1071 - UCGCAGCGGUUCUCGUA 17 1311

HSV2-UL19-1072 - CG CAG CG G U U CU CG U AC 17 1312

HSV2-UL19-2481 - CGGGGACCUGCUGUACA 17 2660

HSV2-UL19-1074 - GGGGACCUGCUGUACAA 17 1314

HSV2-UL19-2483 - CGGGGCCUAUCACCUCA 17 2661

HSV2-UL19-1077 - GGGGCCUAUCACCUCAA 17 1317

HSV2-UL19-2485 - CGGCCAAACAUCGCUGC 17 2662

HSV2-UL19-1082 - GGCCAAACAUCGCUGCC 17 1322

HSV2-UL19-2487 - GCCUGGAGCGUCUCAUC 17 2663

HSV2-UL19-1083 - CCUGGAGCGUCUCAUCG 17 1323

HSV2-UL19-2489 - AGCGUCUCAUCGUGGAA 17 2664

HSV2-UL19-1084 - GCGUCUCAUCGUGGAAA 17 1324

HSV2-UL19-2491 - UGCAGUUUAAGCGCCCG 17 2665

HSV2-UL19-1089 - GCAGUUUAAGCGCCCGC 17 1329

HSV2-UL19-2493 - GCGCCCGCCGGGGUGCC 17 2666

HSV2-UL19-2494 - CGGGGUGCCGCGAGCUC 17 2667

HSV2-UL19-1092 - GGGGUGCCGCGAGCUCG 17 1332

HSV2-UL19-2496 - ACCCGUGCGGCCUGUUU 17 2668

HSV2-UL19-1094 - CCCGUGCGGCCUGU UUC 17 1334

HSV2-UL19-2498 - GCUACGCAGCGCCCGCG 17 2669

HSV2-UL19-1095 - CUACGCAGCGCCCGCGA 17 1335

HSV2-UL19-1096 - UACGCAGCGCCCGCGAU 17 1336

HSV2-UL19-1097 - ACGCAGCGCCCGCGAUG 17 1337

HSV2-UL19-2502 - GAUGGGGAGGCCCACGC 17 2670

HSV2-UL19-2503 - UGGGGAGGCCCACGCGC 17 2671

HSV2-UL19-2504 - ACGACGCCUCCCCGCUA 17 2672

HSV2-UL19-2505 + UUACAGAGACAGGCCCUUUA 20 2673

HSV2-UL19-1101 + UACAGAGACAGGCCCUUUAG 20 1341

HSV2-UL19-1102 + ACAGAGACAGGCCCUUUAGC 20 1342

HSV2-UL19-1103 + CAGAGACAGGCCCUUUAGCG 20 1343

HSV2-UL19-2509 + UUAGCGGGGAGGCGUCGUAG 20 2674

HSV2-UL19-2510 + UAGAUGAGAUACUGCGUAAA 20 2675 HSV2-UL19-2511 + GUAAAGUGGGUCUCUCGCGC 20 2676

HSV2-UL19-2512 + CGCGCGUGGGCCUCCCCAUC 20 2677

HSV2-UL19-2513 + CCAUCGCGGGCGCUGCGUAG 20 2678

HSV2-UL19-2514 + CGCGGGCGCUGCGUAGCAGG 20 2679

HSV2-UL19-1113 + GCGGGCGCUGCGUAGCAGGG 20 1353

HSV2-UL19-2516 + GGGGUCGCUGGCGCAGGUGA 20 2680

HSV2-UL19-2517 + CAGGUGAUCGGGUAGGCUUC 20 2681

HSV2-UL19-2518 + GGCUUCCUGAAACAGGCCGC 20 2682

HSV2-UL19-2519 + ACAGGCCGCACGGGUCUUCC 20 2683

HSV2-UL19-2520 + CACGAGCUCGCGGCACCCCG 20 2684

HSV2-UL19-2521 + CGUCGCUGGCAGCGGUGGCC 20 2685

HSV2-UL19-2522 + CCGCCGAUCCCGUU UCCACG 20 2686

HSV2-UL19-2523 + UGGCCGUGAUGUCGGCCGCG 20 2687

HSV2-UL19-2524 + CCGUGAUGUCGGCCGCGGUG 20 2688

HSV2-UL19-2525 + UGUCGGCCGCGGUGAAGAAC 20 2689

HSV2-UL19-2526 + CCGCGGUGAAGAACUUGAAG 20 2690

HSV2-UL19-1136 + CGCGGUGAAGAACUUGAAGC 20 1376

HSV2-UL19-2528 + UGAAGAACUUGAAGCAGGGG 20 2691

HSV2-UL19-2529 + AAGAACUUGAAGCAGGGGCU 20 2692

HSV2-UL19-2530 + ACUUGAAGCAGGGGCUGAGG 20 2693

HSV2-UL19-2531 + GAAGCAGGGGCUGAGGACGG 20 2694

HSV2-UL19-2532 + UGAGGACGGGCGAGGCCCCG 20 2695

HSV2-UL19-2533 + GUUGUACAGCAGGUCCCCGU 20 2696

HSV2-UL19-2534 + UGUACAGCAGGUCCCCGUAC 20 2697

HSV2-UL19-2535 + CGAGAACCGCUGCGACGCCC 20 2698

HSV2-UL19-2536 + CCCACGGGU UGGCCGUGGCC 20 2699

HSV2-UL19-2537 + ACGGGUUGGCCGUGGCCGCG 20 2700

HSV2-UL19-2538 + CGUGGCCGCGAAGGGCCGCG 20 2701

HSV2-UL19-2539 + CGCUCUGGCCGUGGUCGUAC 20 2702

HSV2-UL19-2540 + CUCUGGCCGUGGUCGUACAU 20 2703

HSV2-UL19-2541 + GCCGCCGGCCGCGCGUCCCC 20 2704

HSV2-UL19-2542 + CCGCGCGUCCCCGCGGGUUG 20 2705

HSV2-UL19-2543 + CCCCGCGGGUUGCAGGGCCG 20 2706

HSV2-UL19-2544 + AGUAGUUGAUGUCCGUGGCC 20 2707

HSV2-UL19-1165 + GUAGUUGAUGUCCGUGGCCA 20 1405

HSV2-UL19-1166 + UAGUUGAUGUCCGUGGCCAC 20 1406

HSV2-UL19-2547 + CCGUGGCCACGGGGGUGGCG 20 2708

HSV2-UL19-2548 + AUGCCGGCGCGCCGCGGCAC 20 2709

HSV2-UL19-2549 + CACCUGGGCG CAG CCAAAG A 20 2710

HSV2-UL19-1176 + ACCUGGGCGCAGCCAAAGAC 20 1416

HSV2-UL19-1177 + CCUGGGCGCAGCCAAAGACC 20 1417

HSV2-UL19-2552 + UGGGCGCAGCCAAAGACCGG 20 2711

HSV2-UL19-1178 + GGGCGCAGCCAAAGACCGGG 20 1418

HSV2-UL19-2554 + CAGCCAAAGACCGGGAGGGG 20 2712

HSV2-UL19-2555 + GGCGGCCGCGUUGUCUAGCA 20 2713

HSV2-UL19-1188 + GCGGCCGCGUUGUCUAGCAG 20 1428 HSV2-UL19-1189 + CGGCCGCGUUGUCUAGCAGC 20 1429

HSV2-UL19-1190 + GGCCGCGUUGUCUAGCAGCG 20 1430

HSV2-UL19-1191 + GCCGCGUUGUCUAGCAGCGG 20 1431

HSV2-UL19-2560 + GCAGCGGGGGGGCCCCGCGG 20 2714

HSV2-UL19-2561 + CCGCGGCCGAGGUAAAAGUU 20 2715

HSV2-UL19-1196 + CGCGGCCGAGGUAAAAGUUU 20 1436

HSV2-UL19-1197 + GCGGCCGAGGUAAAAGUUUU 20 1437

HSV2-UL19-1198 + CGGCCGAGGUAAAAGUUUUG 20 1438

HSV2-UL19-1199 + GGCCGAGGUAAAAGUUUUGG 20 1439

HSV2-UL19-2566 + GGAGGUUGCCCAUGUCCGUA 20 2716

HSV2-UL19-1201 + GAGGUUGCCCAUGUCCGUAA 20 1441

HSV2-UL19-2568 + CCCAUGUCCGUAACGGGGUU 20 2717

HSV2-UL19-2569 + AGGUCCACGUUUCCGCGCGG 20 2718

HSV2-UL19-2570 + CCACGUUUCCGCGCGGCUGG 20 2719

HSV2-UL19-2571 + UUCCGCGCGGCUGGGUGAGC 20 2720

HSV2-UL19-2572 + ACCCCCCCGCCCGUUUCGUG 20 2721

HSV2-UL19-2573 + CCCGUUUCGUGGCGGGCCAC 20 2722

HSV2-UL19-1216 + CCGUUUCGUGGCGGGCCACC 20 1456

HSV2-UL19-2575 + CGGGCCACCUGGAGCUGGCC 20 2723

HSV2-UL19-2576 + AGUACGCCUCCGACGCGCGC 20 2724

HSV2-UL19-1218 + GUACGCCUCCGACGCGCGCU 20 1458

HSV2-UL19-2578 + AAAACAGCACGUUCUCGGUC 20 2725

HSV2-UL19-2579 + AGCGGUCCUGCCGCACGACG 20 2726

HSV2-UL19-2580 + CCUGCCGCACGACGGUGAAC 20 2727

HSV2-UL19-2581 + GCACGACGGUGAACCCGAAC 20 2728

HSV2-UL19-1222 + CACGACG G U G AACCCG AACC 20 1462

HSV2-UL19-2583 + ACGGUGAACCCGAACCCGGG 20 2729

HSV2-UL19-1225 + CGGUGAACCCGAACCCGGGG 20 1465

HSV2-UL19-2585 + ACCCGGGGUGGAGGCCCGUC 20 2730

HSV2-UL19-2586 + CCCGUCUUGAGCUGGUGAUA 20 2731

HSV2-UL19-2587 + UGAGCUGGUGAUACAGGGCC 20 2732

HSV2-UL19-1230 + GAGCUGGUGAUACAGGGCCA 20 1470

HSV2-UL19-2589 + ACAGGGCCACGGGGCUCAUC 20 2733

HSV2-UL19-2590 + UCAUCUUGAAGUACCCCGCC 20 2734

HSV2-UL19-2591 + UUCUCCCCCGCCGCGCUCUC 20 2735

HSV2-UL19-2592 + UCUCGCGGGCGUGCUGCACC 20 2736

HSV2-UL19-2593 + GCGUGCUGCACCACGGGCUG 20 2737

HSV2-UL19-2594 + GCUGCACCACGGGCUGGCGG 20 2738

HSV2-UL19-1240 + CUGCACCACGGGCUGGCGGA 20 1480

HSV2-UL19-2596 + GCACCACGGGCUGGCGGAUG 20 2739

HSV2-UL19-1241 + CACCACGGGCUGGCGGAUGG 20 1481

HSV2-UL19-2598 + CCACGGGCUGGCGGAUGGAG 20 2740

HSV2-UL19-2599 + GAGGAGAAGUAGUUGGCCCC 20 2741

HSV2-UL19-2600 + GAAGUAGUUGGCCCCCAGGG 20 2742

HSV2-UL19-1245 + AAGUAGUUGGCCCCCAGGGC 20 1485

HSV2-UL19-1246 + AGUAGUUGGCCCCCAGGGCC 20 1486 HSV2-UL19-1247 + GUAGUUGGCCCCCAGGGCCG 20 1487

HSV2-UL19-1248 + UAGUUGGCCCCCAGGGCCGG 20 1488

HSV2-UL19-2605 + GCCCCCAGGGCCGGGGGGAC 20 2743

HSV2-UL19-1250 + CCCCCAGGGCCGGGGGGACC 20 1490

HSV2-UL19-1251 + CCCCAGGGCCGGGGGGACCA 20 1491

HSV2-UL19-1252 + CCCAGGGCCGGGGGGACCAG 20 1492

HSV2-UL19-2609 + ACGUCGCGCGCCAGGUCGCG 20 2744

HSV2-UL19-2610 + GCGCGCCAGGUCGCGCAGGG 20 2745

HSV2-UL19-1257 + CGCGCCAGGUCGCGCAGGGC 20 1497

HSV2-UL19-1258 + GCGCCAGGUCGCGCAGGGCC 20 1498

HSV2-UL19-1259 + CGCCAGGUCGCGCAGGGCCG 20 1499

HSV2-UL19-1260 + GCCAGGUCGCGCAGGGCCGG 20 1500

HSV2-UL19-1261 + CCAGGUCGCGCAGGGCCGGG 20 1501

HSV2-UL19-2616 + CGCGCAGGGCCGGGGGGAAG 20 2746

HSV2-UL19-2617 + UCGGCGCCCGCAAACAGCGC 20 2747

HSV2-UL19-2618 + CGCCCGCAAACAGCGCGUGG 20 2748

HSV2-UL19-2619 + GCAAACAGCGCGUGGACGGG 20 2749

HSV2-UL19-2620 + GCGCGUGGACGGGCAGGACG 20 2750

HSV2-UL19-2621 + ACGUAGAAGUAUUCGCCAUU 20 2751

HSV2-UL19-2622 + UGGAUGGUGUGGUCCAGGUG 20 2752

HSV2-UL19-1275 + GGAUGGUGUGGUCCAGGUGC 20 1515

HSV2-UL19-1276 + GAUGGUGUGGUCCAGGUGCU 20 1516

HSV2-UL19-1277 + AUGGUGUGGUCCAGGUGCUG 20 1517

HSV2-UL19-2626 + GGUCCAGGUGCUGGGGGGCC 20 2753

HSV2-UL19-2627 + CGCCGGCGUGCAGCGCCCCG 20 2754

HSV2-UL19-2628 + ACGCGGUGUUGGCGCCCGCG 20 2755

HSV2-UL19-2629 + UGGCGCCCGCGUCGGGCGCC 20 2756

HSV2-UL19-1286 + GGCGCCCGCGUCGGGCGCCG 20 1526

HSV2-UL19-2631 + UGUGCGCCAGCACCUGCAGC 20 2757

HSV2-UL19-2632 + GCACCUGCAGCGUGAGCAUG 20 2758

HSV2-UL19-2633 + UCGACGACGACGCGCCCGUU 20 2759

HSV2-UL19-1291 + CGACGACGACGCGCCCGU UG 20 1531

HSV2-UL19-2635 + CCGUGUUGGCCACCAGAUUG 20 2760

HSV2-UL19-1294 + CGUGUUGGCCACCAGAUUGG 20 1534

HSV2-UL19-2637 + CACCAGAUUGGCGGGAUGCA 20 2761

HSV2-UL19-2638 + AGAUUGGCGGGAUGCAGCGG 20 2762

HSV2-UL19-2639 + UGGCGGGAUGCAGCGGGUGG 20 2763

HSV2-UL19-1300 + GGCGGGAUGCAGCGGGUGGG 20 1540

HSV2-UL19-2641 + GCGGGUGGGCGGGGUCGGUC 20 2764

HSV2-UL19-1304 + CGGGUGGGCGGGGUCGGUCA 20 1544

HSV2-UL19-2643 + GGGGUCGGUCACGGGAUCGC 20 2765

HSV2-UL19-2644 + GAUCGCUCGGGCACUCCUCA 20 2766

HSV2-UL19-1308 + AUCGCUCGGGCACUCCUCAC 20 1548

HSV2-UL19-1309 + UCGCUCGGGCACUCCUCACC 20 1549

HSV2-UL19-2647 + CUCCUCACCGGGGGCGAUCU 20 2767

HSV2-UL19-1312 + UCCUCACCGGGGGCGAUCUC 20 1552 HSV2-UL19-2649 + GCAGCGUGGCGUACACGCGG 20 2768

HSV2-UL19-2650 + GUGGCGUACACGCGGUCGAA 20 2769

HSV2-UL19-2651 + CGCGGUGCAGCAGCGCCCCC 20 2770

HSV2-UL19-2652 + CGGUGCAGCAGCGCCCCCGC 20 2771

HSV2-UL19-2653 + ACGUAAUAGUAGAUUUUGUG 20 2772

HSV2-UL19-2654 + UCGUCGGCGGCGUCGGCCGC 20 2773

HSV2-UL19-2655 + GCGGCGUCGGCCGCGCGGGC 20 2774

HSV2-UL19-2656 + CGUUGCACGCCGCCGCGUAG 20 2775

HSV2-UL19-2657 + CGGCAGUCUCGGUGGCGGUC 20 2776

HSV2-UL19-2658 + UAAGGCCGUCGCAGUCCCAC 20 2777

HSV2-UL19-2659 + AGGCCGUCGCAGUCCCACAC 20 2778

HSV2-UL19-1347 + GGCCGUCGCAGUCCCACACG 20 1587

HSV2-UL19-2661 + CACGAGGGGCGGCAGCAGCG 20 2779

HSV2-UL19-2662 + AUCAGGUGAUUCAGCUCGGC 20 2780

HSV2-UL19-2663 + CUGAGCCUG CCCG CCCAG CU 20 2781

HSV2-UL19-2664 + CCGCCCAGCUCCGGGCCCGG 20 2782

HSV2-UL19-2665 + AGGGUAAAGUCGUCCACCAG 20 2783

HSV2-UL19-2666 + AAGUCGUCCACCAGCUGGGC 20 2784

HSV2-UL19-2667 + AGCUGGGCCAGGGCCUCGAC 20 2785

HSV2-UL19-2668 + GAUACACGGCCAUGCACUCC 20 2786

HSV2-UL19-1368 + AUACACGGCCAUGCACUCCU 20 1608

HSV2-UL19-1369 + UACACGGCCAUGCACUCCUC 20 1609

HSV2-UL19-1370 + ACACGGCCAUGCACUCCUCG 20 1610

HSV2-UL19-2672 + ACUCCUCGGGGAGGUCGCCC 20 2787

HSV2-UL19-2673 + CCCGAGGUAGGUCACGAUGU 20 2788

HSV2-UL19-2674 + GGUCACGAUGUACGAGACCA 20 2789

HSV2-UL19-2675 + AGACCAGCGAGUAGUCGUUC 20 2790

HSV2-UL19-2676 + UUCCAGUAGCUGGUGAUGCA 20 2791

HSV2-UL19-2677 + AGCUGGUGAUGCACUGAGUC 20 2792

HSV2-UL19-2678 + CACUGAGUCACGAGCCGCGC 20 2793

HSV2-UL19-2679 + UCACGAGCCGCGCCAGGGCG 20 2794

HSV2-UL19-2680 + CAGAACACGUGCUCGUUGCC 20 2795

HSV2-UL19-2681 + UUGCAGCAGGU AAAACACCG 20 2796

HSV2-UL19-2682 + CCGCCGGGUAGCUGCGGUCC 20 2797

HSV2-UL19-2683 + CUGCGGUCCUCGAACGCCCC 20 2798

HSV2-UL19-2684 + CCAUGGCGUGGCGGCCAACG 20 2799

HSV2-UL19-2685 + CCAACGCCGAGCUCCAGGCC 20 2800

HSV2-UL19-2686 + AGCUCCAGGCCCCGGGCGUC 20 2801

HSV2-UL19-2687 + CCGGGCGUCACG AAACG CCA 20 2802

HSV2-UL19-2688 + AACGCCACCGGACACAGCGC 20 2803

HSV2-UL19-1394 + ACGCCACCGGACACAGCGCC 20 1634

HSV2-UL19-2690 + UUGACCACGCGCCAGGUGGC 20 2804

HSV2-UL19-2691 + CCAGGUGGCCUGGAUCGCCC 20 2805

HSV2-UL19-2692 + CUGGAUCGCCCCCGGACCGG 20 2806

HSV2-UL19-1403 + UGGAUCGCCCCCGGACCGGC 20 1643

HSV2-UL19-1404 + GGAUCGCCCCCGGACCGGCC 20 1644 HSV2-UL19-1405 + GAUCGCCCCCGGACCGGCCG 20 1645

HSV2-UL19-1406 + AUCGCCCCCGGACCGGCCGG 20 1646

HSV2-UL19-2697 + CGGCCGGGGGGACUUCGCCG 20 2807

HSV2-UL19-1408 + GGCCGGGGGGACUUCGCCGC 20 1648

HSV2-UL19-1409 + GCCGGGGGGACUUCGCCGCC 20 1649

HSV2-UL19-2700 + GCUCGACGUCGGCCACGCCC 20 2808

HSV2-UL19-2701 + CG ACG U CGGCCACG CCCG CG 20 2809

HSV2-UL19-2702 + CG G CCACG CCCGCG AAG AAG 20 2810

HSV2-UL19-2703 + CGCCCGCGAAGAAGUCGAAC 20 2811

HSV2-UL19-1411 + GCCCGCGAAGAAGUCGAACG 20 1651

HSV2-UL19-2705 + UCGAACGCGGGGUGCAGCUC 20 2812

HSV2-UL19-2706 + CCAGAGCCAGGUUGGCGUUG 20 2813

HSV2-UL19-2707 + GGUUGGCGUUGUCGGGCUGC 20 2814

HSV2-UL19-2708 + AUCUGGCACUCGGCGACCCA 20 2815

HSV2-UL19-2709 + GCGACCCACCGGACCCGGCC 20 2816

HSV2-UL19-2710 + CCCACCGGACCCGGCCGUGG 20 2817

HSV2-UL19-2711 + AGGCGCUGCCGCCAGGCGUU 20 2818

HSV2-UL19-2712 + AACGCUGCUGCAUGUCCGCG 20 2819

HSV2-UL19-1427 + ACGCUGCUGCAUGUCCGCGC 20 1667

HSV2-UL19-2714 + CAUGUCCGCGCCGGGGCCGG 20 2820

HSV2-UL19-1431 + AUGUCCGCGCCGGGGCCGGC 20 1671

HSV2-UL19-2716 + GGCCGCGACGUACGCCCCGU 20 2821

HSV2-UL19-2717 + CGUACGGAUUCGCGGCCUCG 20 2822

HSV2-UL19-1436 + GUACGGAUUCGCGGCCUCGA 20 1676

HSV2-UL19-2719 + CGACGGCCGCGUCGAUGUUC 20 2823

HSV2-UL19-2720 + GGCCGCGUCGAUGUUCAUGA 20 2824

HSV2-UL19-2721 + CGCGUCGAUGUUCAUGAGCG 20 2825

HSV2-UL19-2722 + CGGUCCCGACCGCGU UCUCC 20 2826

HSV2-UL19-2723 + GCGUUCUCCAUGGACAGCCG 20 2827

HSV2-UL19-2724 + U U UCCCCAAAAAAACAGCUG 20 2828

HSV2-UL19-1446 + UUCCCCAAAAAAACAGCUGC 20 1686

HSV2-UL19-1447 + UCCCCAAAAAAACAGCUGCC 20 1687

HSV2-UL19-1448 + CCCCAAA AAAACAG CU G CCG 20 1688

HSV2-UL19-2728 + CCAA AAAAACAG CU G CCG G G 20 2829

HSV2-UL19-1449 + CAAAAAAACAGCUGCCGGGG 20 1689

HSV2-UL19-1450 + AAAAAAACAGCUGCCGGGGA 20 1690

HSV2-UL19-2731 + AGCUGCCGGGGAGGGAACGC 20 2830

HSV2-UL19-1451 + GCUGCCGGGGAGGGAACGCG 20 1691

HSV2-UL19-2733 + GGGAGGGAACGCGCGGGGCU 20 2831

HSV2-UL19-2734 + ACGCGCGGGGCUCCGGGUGG 20 2832

HSV2-UL19-1457 + CGCGCGGGGCUCCGGGUGGC 20 1697

HSV2-UL19-1458 + GCGCGGGGCUCCGGGUGGCC 20 1698

HSV2-UL19-2737 + GGGGCUCCGGGUGGCCGGGG 20 2833

HSV2-UL19-2738 + CCAGGUCCCCGGCGUGCGCG 20 2834

HSV2-UL19-2739 + CGCGGCGAAGCGCUCCAUGG 20 2835

HSV2-UL19-2740 + CGAAGCGCUCCAUGGCCGGG 20 2836 HSV2-UL19-2741 + AUGGCCGGGUUGAACAGCCC 20 2837

HSV2-UL19-1469 + UGGCCGGGUUGAACAGCCCC 20 1709

HSV2-UL19-2743 + GGGUUGAACAGCCCCAGGGG 20 2838

HSV2-UL19-2744 + UGAACAGCCCCAGGGGCAGG 20 2839

HSV2-UL19-2745 + GUCAGGUCCAUGGCGCCCAC 20 2840

HSV2-UL19-1475 + UCAGGUCCAUGGCGCCCACC 20 1715

HSV2-UL19-1476 + CAGGUCCAUGGCGCCCACCA 20 1716

HSV2-UL19-2748 + CCAUGGCGCCCACCAGGGGG 20 2841

HSV2-UL19-2749 + GCGGCGUAGAUGCGUCUCUC 20 2842

HSV2-UL19-2750 + AUGCGUCUCUCCAGGGCCUC 20 2843

HSV2-UL19-1486 + UGCGUCUCUCCAGGGCCUCC 20 1726

HSV2-UL19-2752 + UCCGCGCGCACGCGCGUUGU 20 2844

HSV2-UL19-1488 + CCGCGCGCACGCGCGUUGUC 20 1728

HSV2-UL19-1489 + CGCGCGCACGCGCGUUGUCU 20 1729

HSV2-UL19-1490 + GCGCGCACGCGCGUUGUCUG 20 1730

HSV2-UL19-2756 + GCGUUGUCUGGGGGGCGCUU 20 2845

HSV2-UL19-2757 + CCAGCGUCUCCCGGUUCGCC 20 2846

HSV2-UL19-2758 + UGCCCAUCACCAGCGCCGUG 20 2847

HSV2-UL19-2759 + GCCUCGGUGUCCUCCACCUU 20 2848

HSV2-UL19-1505 + CCUCGGUGUCCUCCACCUUC 20 1745

HSV2-UL19-2761 + CGGUGUCCUCCACCUUCAGG 20 2849

HSV2-UL19-2762 + UCCACCUUCAGGAAGGACUG 20 2850

HSV2-UL19-1507 + CCACCUUCAGGAAGGACUGC 20 1747

HSV2-UL19-2764 + CGCGUGUCGGCGUGCGUCAG 20 2851

HSV2-UL19-1516 + GCGUGUCGGCGUGCGUCAGG 20 1756

HSV2-UL19-2766 + GCGUCAGGCGGGGCACGGCC 20 2852

HSV2-UL19-2767 + GCGUCGCCGUGGUCAGGUCC 20 2853

HSV2-UL19-2768 + CGGCGAUGGCCCGCCUUGCC 20 2854

HSV2-UL19-1528 + GGCGAUGGCCCGCCUUGCCC 20 1768

HSV2-UL19-2770 + GAUGGCCCGCCUUGCCCAGG 20 2855

HSV2-UL19-2771 + CUCCGCUUCAGCUCGGCGAC 20 2856

HSV2-UL19-2772 + AGCUCGGCGACCAGGGUCGC 20 2857

HSV2-UL19-2773 + UGGUCGCCAGGCGCCCGUUG 20 2858

HSV2-UL19-2774 + CGUUGUCGAGAUAUCGUUGC 20 2859

HSV2-UL19-2775 + UAUCGUUGCAUGGGCAACAG 20 2860

HSV2-UL19-2776 + UGCAUGGGCAACAGCAGGGC 20 2861

HSV2-UL19-1540 + G CA U G G G CAACAG CAGG G CC 20 1780

HSV2-UL19-1541 + CA U G G G CAACAG CAG G G CCA 20 1781

HSV2-UL19-1542 + AUGGGCAACAGCAGGGCCAG 20 1782

HSV2-UL19-2780 + GUGCCGCGCUCAAACGCCCC 20 2862

HSV2-UL19-2781 + UCAAACGCCCCCAGGACGGC 20 2863

HSV2-UL19-2782 + CAGAGACAGGCCCUUUA 17 2864

HSV2-UL19-1549 + AGAGACAGGCCCUUUAG 17 1789

HSV2-UL19-1550 + GAGACAGGCCCUUUAGC 17 1790

HSV2-UL19-1551 + AGACAGGCCCUUUAGCG 17 1791

HSV2-UL19-2786 + GCGGGGAGGCGUCGUAG 17 2865 HSV2-UL19-2787 + AUGAGAUACUGCGUAAA 17 2866

HSV2-UL19-2788 + AAGUGGGUCUCUCGCGC 17 2867

HSV2-UL19-2789 + GCGUGGGCCUCCCCAUC 17 2868

HSV2-UL19-2790 + UCGCGGGCGCUGCGUAG 17 2869

HSV2-UL19-2791 + GGGCGCUGCGUAGCAGG 17 2870

HSV2-UL19-1561 + GGCGCUGCGUAGCAGGG 17 1801

HSV2-UL19-2793 + GUCGCUGGCGCAGGUGA 17 2871

HSV2-UL19-2794 + GUGAUCGGGUAGGCUUC 17 2872

HSV2-UL19-2795 + UUCCUGAAACAGGCCGC 17 2873

HSV2-UL19-2796 + GGCCGCACGGGUCUUCC 17 2874

HSV2-UL19-2797 + GAGCUCGCGGCACCCCG 17 2875

HSV2-UL19-2798 + CGCUGGCAGCGGUGGCC 17 2876

HSV2-UL19-2799 + CCGAUCCCGU UUCCACG 17 2877

HSV2-UL19-2800 + CCGUGAUGUCGGCCGCG 17 2878

HSV2-UL19-2801 + UGAUGUCGGCCGCGGUG 17 2879

HSV2-UL19-2802 + CGGCCGCGGUGAAGAAC 17 2880

HSV2-UL19-2803 + CGGUGAAGAACUUGAAG 17 2881

HSV2-UL19-1584 + GGUGAAGAACUUGAAGC 17 1824

HSV2-UL19-2805 + AGAACUUGAAGCAGGGG 17 2882

HSV2-UL19-2806 + AACUUGAAGCAGGGGCU 17 2883

HSV2-UL19-2807 + UGAAGCAGGGGCUGAGG 17 2884

HSV2-UL19-2808 + GCAGGGGCUGAGGACGG 17 2885

HSV2-UL19-2809 + GGACGGGCGAGGCCCCG 17 2886

HSV2-UL19-2810 + GUACAGCAGGUCCCCGU 17 2887

HSV2-UL19-2811 + ACAGCAGGUCCCCGUAC 17 2888

HSV2-UL19-2812 + GAACCGCUGCGACGCCC 17 2889

HSV2-UL19-2813 + ACGGGUUGGCCGUGGCC 17 2890

HSV2-UL19-2814 + GGUUGGCCGUGGCCGCG 17 2891

HSV2-UL19-2815 + GGCCGCGAAGGGCCGCG 17 2892

HSV2-UL19-2816 + UCUGGCCGUGGUCGUAC 17 2893

HSV2-UL19-2817 + UGGCCGUGGUCGUACAU 17 2894

HSV2-UL19-2818 + GCCGGCCGCGCGUCCCC 17 2895

HSV2-UL19-2819 + CGCGUCCCCGCGGGUUG 17 2896

HSV2-UL19-2820 + CGCGGGUUGCAGGGCCG 17 2897

HSV2-UL19-2821 + AGUUGAUGUCCGUGGCC 17 2898

HSV2-UL19-1613 + GUUGAUGUCCGUGGCCA 17 1853

HSV2-UL19-1614 + UUGAUGUCCGUGGCCAC 17 1854

HSV2-UL19-2824 + UGGCCACGGGGGUGGCG 17 2899

HSV2-UL19-2825 + CCGGCGCGCCGCGGCAC 17 2900

HSV2-UL19-2826 + CUGGGCGCAG CCAAAG A 17 2901

HSV2-UL19-1624 + UGGGCGCAG CCAAAG AC 17 1864

HSV2-UL19-1625 + GGGCGCAGCCAAAGACC 17 1865

HSV2-UL19-2829 + GCGCAGCCAAAGACCGG 17 2902

HSV2-UL19-1626 + CGCAGCCAAAGACCGGG 17 1866

HSV2-UL19-2831 + CCAAAG ACCGGGAGGGG 17 2903

HSV2-UL19-2832 + GGCCGCGUUGUCUAGCA 17 2904 HSV2-UL19-1636 + GCCGCGUUGUCUAGCAG 17 1876

HSV2-UL19-1637 + CCGCGUUGUCUAGCAGC 17 1877

HSV2-UL19-1638 + CGCGU UGUCUAGCAGCG 17 1878

HSV2-UL19-1639 + GCGUUGUCUAGCAGCGG 17 1879

HSV2-UL19-2837 + GCGGGGGGGCCCCGCGG 17 2905

HSV2-UL19-2838 + CGGCCGAGGUAAAAGUU 17 2906

HSV2-UL19-1644 + GGCCGAGGUAAAAGUUU 17 1884

HSV2-UL19-1645 + GCCGAGGUAAAAGUUUU 17 1885

HSV2-UL19-1646 + CCGAGGUAAAAGUUUUG 17 1886

HSV2-UL19-1647 + CGAGGUAAAAGUUUUGG 17 1887

HSV2-UL19-2843 + GGUUGCCCAUGUCCGUA 17 2907

HSV2-UL19-1649 + GUUGCCCAUGUCCGUAA 17 1889

HSV2-UL19-2845 + AUGUCCGUAACGGGGUU 17 2908

HSV2-UL19-2846 + UCCACGUUUCCGCGCGG 17 2909

HSV2-UL19-2847 + CGUUUCCGCGCGGCUGG 17 2910

HSV2-UL19-2848 + CGCGCGGCUGGGUGAGC 17 2911

HSV2-UL19-2849 + CCCCCGCCCGUUUCGUG 17 2912

HSV2-UL19-2850 + GUUUCGUGGCGGGCCAC 17 2913

HSV2-UL19-1664 + UUUCGUGGCGGGCCACC 17 1904

HSV2-UL19-2852 + GCCACCUGGAGCUGGCC 17 2914

HSV2-UL19-2853 + ACGCCUCCGACGCGCGC 17 2915

HSV2-UL19-1666 + CGCCUCCGACGCGCGCU 17 1906

HSV2-UL19-2855 + ACAGCACGUUCUCGGUC 17 2916

HSV2-UL19-2856 + GGUCCUGCCGCACGACG 17 2917

HSV2-UL19-2857 + GCCGCACGACGGUGAAC 17 2918

HSV2-UL19-2858 + CGACGGUGAACCCGAAC 17 2919

HSV2-UL19-1670 + GACG G U G AACCCG AACC 17 1910

HSV2-UL19-2860 + GUGAACCCGAACCCGGG 17 2920

HSV2-UL19-1673 + UGAACCCGAACCCGGGG 17 1913

HSV2-UL19-2862 + CGGGGUGGAGGCCCGUC 17 2921

HSV2-UL19-2863 + GUCUUGAGCUGGUGAUA 17 2922

HSV2-UL19-2864 + GCUGGUGAUACAGGGCC 17 2923

HSV2-UL19-1678 + CUGGUGAUACAGGGCCA 17 1918

HSV2-UL19-2866 + GGGCCACGGGGCUCAUC 17 2924

HSV2-UL19-2867 + UCUUGAAGUACCCCGCC 17 2925

HSV2-UL19-2868 + UCCCCCGCCGCGCUCUC 17 2926

HSV2-UL19-2869 + CGCGGGCGUGCUGCACC 17 2927

HSV2-UL19-2870 + UGCUGCACCACGGGCUG 17 2928

HSV2-UL19-2871 + GCACCACGGGCUGGCGG 17 2929

HSV2-UL19-1688 + CACCACGGGCUGGCGGA 17 1928

HSV2-UL19-2873 + CCACGGGCUGGCGGAUG 17 2930

HSV2-UL19-1689 + CACGGGCUGGCGGAUGG 17 1929

HSV2-UL19-2875 + CGGGCUGGCGGAUGGAG 17 2931

HSV2-UL19-2876 + GAGAAGUAGUUGGCCCC 17 2932

HSV2-UL19-2877 + GUAGUUGGCCCCCAGGG 17 2933

HSV2-UL19-1693 + UAGUUGGCCCCCAGGGC 17 1933 HSV2-UL19-1694 + AGUUGGCCCCCAGGGCC 17 1934

HSV2-UL19-1695 + GUUGGCCCCCAGGGCCG 17 1935

HSV2-UL19-1696 + UUGGCCCCCAGGGCCGG 17 1936

HSV2-UL19-2882 + CCCAGGGCCGGGGGGAC 17 2934

HSV2-UL19-1698 + CCAGGGCCGGGGGGACC 17 1938

HSV2-UL19-1699 + CAGGGCCGGGGGGACCA 17 1939

HSV2-UL19-1700 + AGGGCCGGGGGGACCAG 17 1940

HSV2-UL19-2886 + UCGCGCGCCAGGUCGCG 17 2935

HSV2-UL19-2887 + CGCCAGGUCGCGCAGGG 17 2936

HSV2-UL19-1705 + GCCAGGUCGCGCAGGGC 17 1945

HSV2-UL19-1706 + CCAGGUCGCGCAGGGCC 17 1946

HSV2-UL19-1707 + CAGGUCGCGCAGGGCCG 17 1947

HSV2-UL19-1708 + AGGUCGCGCAGGGCCGG 17 1948

HSV2-UL19-1709 + GGUCGCGCAGGGCCGGG 17 1949

HSV2-UL19-2893 + GCAGGGCCGGGGGGAAG 17 2937

HSV2-UL19-2894 + GCGCCCGCAAACAGCGC 17 2938

HSV2-UL19-2895 + CCGCAAACAGCGCGUGG 17 2939

HSV2-UL19-2896 + AACAGCGCGUGGACGGG 17 2940

HSV2-UL19-2897 + CGUGGACGGGCAGGACG 17 2941

HSV2-UL19-2898 + UAGAAGUAUUCGCCAUU 17 2942

HSV2-UL19-2899 + AUGGUGUGGUCCAGGUG 17 2943

HSV2-UL19-1723 + UGGUGUGGUCCAGGUGC 17 1963

HSV2-UL19-1724 + GGUGUGGUCCAGGUGCU 17 1964

HSV2-UL19-1725 + GUGUGGUCCAGGUGCUG 17 1965

HSV2-UL19-2903 + CCAGGUGCUGGGGGGCC 17 2944

HSV2-UL19-2904 + CGGCGUGCAGCGCCCCG 17 2945

HSV2-UL19-2905 + CGGUGUUGGCGCCCGCG 17 2946

HSV2-UL19-2906 + CGCCCGCGUCGGGCGCC 17 2947

HSV2-UL19-1734 + GCCCGCGUCGGGCGCCG 17 1974

HSV2-UL19-2908 + GCGCCAGCACCUGCAGC 17 2948

HSV2-UL19-2909 + CCUGCAGCGUGAGCAUG 17 2949

HSV2-UL19-2910 + ACGACGACG CGCCCG U U 17 2950

HSV2-UL19-1739 + CGACGACGCGCCCGUUG 17 1979

HSV2-UL19-2912 + UGUUGGCCACCAGAUUG 17 2951

HSV2-UL19-1742 + GUUGGCCACCAGAUUGG 17 1982

HSV2-UL19-2914 + CAGAUUGGCGGGAUGCA 17 2952

HSV2-UL19-2915 + UUGGCGGGAUGCAGCGG 17 2953

HSV2-UL19-2916 + CGGGAUGCAGCGGGUGG 17 2954

HSV2-UL19-1748 + GGGAUGCAGCGGGUGGG 17 1988

HSV2-UL19-2918 + GGUGGGCGGGGUCGGUC 17 2955

HSV2-UL19-1752 + GUGGGCGGGGUCGGUCA 17 1992

HSV2-UL19-2920 + GUCGGUCACGGGAUCGC 17 2956

HSV2-UL19-2921 + CGCUCGGGCACUCCUCA 17 2957

HSV2-UL19-1756 + GCUCGGGCACUCCUCAC 17 1996

HSV2-UL19-1757 + CUCGGGCACUCCUCACC 17 1997

HSV2-UL19-2924 + CUCACCGGGGGCGAUCU 17 2958 HSV2-UL19-1760 + UCACCGGGGGCGAUCUC 17 2000

HSV2-UL19-2926 + GCGUGGCGUACACGCGG 17 2959

HSV2-UL19-2927 + GCGUACACGCGGUCGAA 17 2960

HSV2-UL19-2928 + GGUGCAGCAGCGCCCCC 17 2961

HSV2-UL19-2929 + UGCAGCAGCGCCCCCGC 17 2962

HSV2-UL19-2930 + UAAUAGUAGAUUUUGUG 17 2963

HSV2-UL19-2931 + UCGGCGGCGUCGGCCGC 17 2964

HSV2-UL19-2932 + GCGUCGGCCGCGCGGGC 17 2965

HSV2-UL19-2933 + UGCACGCCGCCGCGUAG 17 2966

HSV2-UL19-2934 + CAGUCUCGGUGGCGGUC 17 2967

HSV2-UL19-2935 + GGCCGUCGCAGUCCCAC 17 2968

HSV2-UL19-2936 + CCGUCGCAGU CCCACAC 17 2969

HSV2-UL19-1795 + CG U CG CAG U CCCACACG 17 2035

HSV2-UL19-2938 + GAGGGGCGGCAGCAGCG 17 2970

HSV2-UL19-2939 + AGGUGAUUCAGCUCGGC 17 2971

HSV2-UL19-2940 + AGCCUGCCCGCCCAGCU 17 2972

HSV2-UL19-2941 + CCCAGCUCCGGGCCCGG 17 2973

HSV2-UL19-2942 + G U AAAG UCGUCCACCAG 17 2974

HSV2-UL19-2943 + UCGUCCACCAGCUGGGC 17 2975

HSV2-UL19-2944 + UGGGCCAGGGCCUCGAC 17 2976

HSV2-UL19-2945 + ACACGGCCAUGCACUCC 17 2977

HSV2-UL19-1816 + CACGGCCAUGCACUCCU 17 2056

HSV2-UL19-1817 + ACGGCCAUGCACUCCUC 17 2057

HSV2-UL19-1818 + CGGCCAUGCACUCCUCG 17 2058

HSV2-UL19-2949 + CCUCGGGGAGGUCGCCC 17 2978

HSV2-UL19-2950 + GAGGUAGGUCACGAUGU 17 2979

HSV2-UL19-2951 + CACGAUGUACGAGACCA 17 2980

HSV2-UL19-2952 + CCAGCGAGUAGUCGUUC 17 2981

HSV2-UL19-2953 + CAGUAGCUGGUGAUGCA 17 2982

HSV2-UL19-2954 + UGGUGAUGCACUGAGUC 17 2983

HSV2-UL19-2955 + U G AG U CACG AG CCG CGC 17 2984

HSV2-UL19-2956 + CGAGCCGCGCCAGGGCG 17 2985

HSV2-UL19-2957 + AACACGUGCUCGUUGCC 17 2986

HSV2-UL19-2958 + CAG CAG G U AAAACACCG 17 2987

HSV2-UL19-2959 + CCGGGUAGCUGCGGUCC 17 2988

HSV2-UL19-2960 + CGGUCCUCGAACGCCCC 17 2989

HSV2-UL19-2961 + UGGCGUGGCGGCCAACG 17 2990

HSV2-UL19-2962 + ACGCCGAGCUCCAGGCC 17 2991

HSV2-UL19-2963 + UCCAGGCCCCGGGCGUC 17 2992

HSV2-UL19-2964 + G G CG U CACG AAACG CCA 17 2993

HSV2-UL19-2965 + GCCACCGGACACAGCGC 17 2994

HSV2-UL19-1842 + CCACCGGACACAGCGCC 17 2082

HSV2-UL19-2967 + ACCACGCGCCAGGUGGC 17 2995

HSV2-UL19-2968 + GGUGGCCUGGAUCGCCC 17 2996

HSV2-UL19-2969 + GAUCGCCCCCGGACCGG 17 2997

HSV2-UL19-1851 + AUCGCCCCCGGACCGGC 17 2091 HSV2-UL19-1852 + UCGCCCCCGGACCGGCC 17 2092

HSV2-UL19-1853 + CGCCCCCGGACCGGCCG 17 2093

HSV2-UL19-1854 + GCCCCCGGACCGGCCGG 17 2094

HSV2-UL19-2974 + CCGGGGGGACUUCGCCG 17 2998

HSV2-UL19-1856 + CGGGGGGACUUCGCCGC 17 2096

HSV2-UL19-1857 + GGGGGGACUUCGCCGCC 17 2097

HSV2-UL19-2977 + CGACGUCGGCCACGCCC 17 2999

HSV2-UL19-2978 + CGUCGGCCACGCCCGCG 17 3000

HSV2-UL19-2979 + CCACGCCCGCGAAGAAG 17 3001

HSV2-UL19-2980 + CCGCGAAGAAGUCGAAC 17 3002

HSV2-UL19-1859 + CG CGAAGAAG U CGAACG 17 2099

HSV2-UL19-2982 + AACGCGGGGUGCAGCUC 17 3003

HSV2-UL19-2983 + GAGCCAGGUUGGCGUUG 17 3004

HSV2-UL19-2984 + UGGCGUUGUCGGGCUGC 17 3005

HSV2-UL19-2985 + UGGCACUCGGCGACCCA 17 3006

HSV2-UL19-2986 + ACCCACCGGACCCGGCC 17 3007

HSV2-UL19-2987 + ACCGGACCCGGCCGUGG 17 3008

HSV2-UL19-2988 + CGCUGCCGCCAGGCGU U 17 3009

HSV2-UL19-2989 + GCUGCUGCAUGUCCGCG 17 3010

HSV2-UL19-1875 + CUGCUGCAUGUCCGCGC 17 2115

HSV2-UL19-2991 + GUCCGCGCCGGGGCCGG 17 3011

HSV2-UL19-1879 + UCCGCGCCGGGGCCGGC 17 2119

HSV2-UL19-2993 + CGCGACGUACGCCCCGU 17 3012

HSV2-UL19-2994 + ACGGAUUCGCGGCCUCG 17 3013

HSV2-UL19-1884 + CGGAUUCGCGGCCUCGA 17 2124

HSV2-UL19-2996 + CGGCCGCGUCGAUGUUC 17 3014

HSV2-UL19-2997 + CGCGUCGAUGUUCAUGA 17 3015

HSV2-UL19-2998 + GUCGAUGUUCAUGAGCG 17 3016

HSV2-UL19-2999 + UCCCGACCGCGUUCUCC 17 3017

HSV2-UL19-3000 + UUCUCCAUGGACAGCCG 17 3018

HSV2-UL19-3001 + CCCCAAAAAAACAG CU G 17 3019

HSV2-UL19-1894 + CCCAAAAAAACAG CU G C 17 2134

HSV2-UL19-1895 + CCAAAAAAACAG CU G CC 17 2135

HSV2-UL19-1896 + CA AAAAAACAG CU G CCG 17 2136

HSV2-UL19-3005 + AAAAAACAGCUGCCGGG 17 3020

HSV2-UL19-1897 + AAAAACAG CU G CCG G G G 17 2137

HSV2-UL19-1898 + AAAACAG CU G CCG G G G A 17 2138

HSV2-UL19-3008 + UGCCGGGGAGGGAACGC 17 3021

HSV2-UL19-1899 + GCCGGGGAGGGAACGCG 17 2139

HSV2-UL19-3010 + AGGGAACGCGCGGGGCU 17 3022

HSV2-UL19-3011 + CGCGGGGCUCCGGGUGG 17 3023

HSV2-UL19-1905 + GCGGGGCUCCGGGUGGC 17 2145

HSV2-UL19-1906 + CGGGGCUCCGGGUGGCC 17 2146

HSV2-UL19-3014 + GCUCCGGGUGGCCGGGG 17 3024

HSV2-UL19-3015 + GGUCCCCGGCGUGCGCG 17 3025

HSV2-UL19-3016 + GGCGAAGCGCUCCAUGG 17 3026 HSV2-UL19-3017 + AGCGCUCCAUGGCCGGG 17 3027

HSV2-UL19-3018 + GCCGGGUUGAACAGCCC 17 3028

HSV2-UL19-1917 + CCGGGU UGAACAGCCCC 17 2157

HSV2-UL19-3020 + UUGAACAGCCCCAGGGG 17 3029

HSV2-UL19-3021 + ACAG CCCCAG G G G CAG G 17 3030

HSV2-UL19-3022 + AGGUCCAUGGCGCCCAC 17 3031

HSV2-UL19-1923 + GGUCCAUGGCGCCCACC 17 2163

HSV2-UL19-1924 + GUCCAUGGCGCCCACCA 17 2164

HSV2-UL19-3025 + UGGCGCCCACCAGGGGG 17 3032

HSV2-UL19-3026 + GCGUAGAUGCGUCUCUC 17 3033

HSV2-UL19-3027 + CGUCUCUCCAGGGCCUC 17 3034

HSV2-UL19-1934 + GUCUCUCCAGGGCCUCC 17 2174

HSV2-UL19-3029 + GCGCGCACGCGCGUUGU 17 3035

HSV2-UL19-1936 + CGCGCACGCGCGUUGUC 17 2176

HSV2-UL19-1937 + GCGCACGCGCGUUGUCU 17 2177

HSV2-UL19-1938 + CGCACGCGCGUUGUCUG 17 2178

HSV2-UL19-3033 + UUGUCUGGGGGGCGCUU 17 3036

HSV2-UL19-3034 + GCGUCUCCCGGUUCGCC 17 3037

HSV2-UL19-3035 + CCAU CACCAG CG CCG U G 17 3038

HSV2-UL19-3036 + U CG G U G U CCU CCACCU U 17 3039

HSV2-UL19-1953 + CG G U G U CCU CCACCU U C 17 2193

HSV2-UL19-3038 + UGUCCUCCACCUUCAGG 17 3040

HSV2-UL19-3039 + ACCUUCAGGAAGGACUG 17 3041

HSV2-UL19-1955 + CCUUCAGGAAGGACUGC 17 2195

HSV2-UL19-3041 + GUGUCGGCGUGCGUCAG 17 3042

HSV2-UL19-1964 + UGUCGGCGUGCGUCAGG 17 2204

HSV2-UL19-3043 + UCAGGCGGGGCACGGCC 17 3043

HSV2-UL19-3044 + UCGCCGUGGUCAGGUCC 17 3044

HSV2-UL19-3045 + CGAUGGCCCGCCUUGCC 17 3045

HSV2-UL19-1976 + GAUGGCCCGCCUUGCCC 17 2216

HSV2-UL19-3047 + GGCCCGCCUUGCCCAGG 17 3046

HSV2-UL19-3048 + CGCUUCAGCUCGGCGAC 17 3047

HSV2-UL19-3049 + UCGGCGACCAGGGUCGC 17 3048

HSV2-UL19-3050 + UCGCCAGGCGCCCGUUG 17 3049

HSV2-UL19-3051 + UGUCGAGAUAUCGUUGC 17 3050

HSV2-UL19-3052 + CGU UGCAUGGG CAACAG 17 3051

HSV2-UL19-3053 + AUGGGCAACAGCAGGGC 17 3052

HSV2-UL19-1988 + U G G G CAACAG CAG G G CC 17 2228

HSV2-UL19-1989 + G G G CAACAG C AG G G CCA 17 2229

HSV2-UL19-1990 + G G CAACAG CAG G G CCAG 17 2230

HSV2-UL19-3057 + CCGCGCUCAAACGCCCC 17 3053

HSV2-UL19-3058 + AACGCCCCCAGGACGGC 17 3054

Table IF provides exemplary targeting domains for knocking out the UL19 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table IF

Table 1G provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence, but downstream of the first 500bp of the HSV gene UL19 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 1G

Length

HSV2-UL19-3059 - GGUGUUUUACCUGCUGCAAG 20 3057

HSV2-UL19-3060 - CCUGGACCGCCACCGAGACU 20 3058

HSV2-UL19-3061 - GUUUUACCUGCUGCAAG 17 3059

HSV2-UL19-3062 - GGACCGCCACCGAGACU 17 3060

HSV2-UL19-3063 + UGCGCUUGACCGUGUUGGCC 20 3061

HSV2-UL19-3064 + G G CCG G CACCAG CACG U AAU 20 3062

HSV2-UL19-1353 + GCAGCGCCGGGUCGCGCAUC 20 1593

HSV2-UL19-3066 + GGGCCGCGACGUACGCCCCG 20 3063

HSV2-UL19-3067 + GCUUGACCGUGUUGGCC 17 3064

HSV2-UL19-3068 + CGGCACCAGCACGUAAU 17 3065

HSV2-UL19-1801 + GCGCCGGGUCGCGCAUC 17 2041

HSV2-UL19-3070 + CCGCGACGUACGCCCCG 17 3066

Table 2A provides exemplary targeting domains for knocking out the UL30 gene selected according to first tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL30 gene and orthogonality against the human genome. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2 A

HSV2-UL30-37 - CCCCUAAGGUGUACUGCGGG 20 3074

HSV2-UL30-38 - CCCUAAGGUGUACUGCGGGG 20 3075

HSV2-UL30-39 - CGAGCGCGACGUCCUCCGCG 20 3076

HSV2-UL30-40 - GAGCGCGACGUCCUCCGCGU 20 3077

HSV2-UL30-43 - GUCCUCCGCGUGGGCCCGGA 20 3078

HSV2-UL30-46 - GCCGCGUCGCUUGCGCCUGU 20 3079

HSV2-UL30-47 - CCGCGUCGCUUGCGCCUGUG 20 3080

HSV2-UL30-48 - CGUCGCU UGCGCCUGUGGGG 20 3081

HSV2-UL30-50 - CGGUGCGGACCAUGCCCCCG 20 3082

HSV2-UL30-51 - GGUGCGGACCAUGCCCCCGA 20 3083

HSV2-UL30-53 - CUUCCACGUGUACGACAUCC 20 3084

HSV2-UL30-60 + ACGUGGAAGACGGUGACGGU 20 3085

HSV2-UL30-62 + GACGGUGGGGUCGAACCCCU 20 3086

HSV2-UL30-63 + ACGGUGGGGUCGAACCCCUC 20 3087

HSV2-UL30-64 + CGGUGGGGUCGAACCCCUCG 20 3088

HSV2-UL30-65 + GGUGGGGUCGAACCCCUCGG 20 3089

HSV2-UL30-66 + GGUCGAACCCCUCGGGGGCA 20 3090

HSV2-UL30-68 + CCCCACAGGCGCAAGCGACG 20 3091

HSV2-UL30-69 + CGACGCGGCCAGAAGCCCUC 20 3092

HSV2-UL30-74 + CCCCCCCGCAG UACACCU UA 20 3093

HSV2-UL30-75 + CCCCCCGCAG UACACCU U AG 20 3094

HSV2-UL30-76 + AGUACACCUUAGGGGCGCGC 20 3095

HSV2-UL30-77 + ACACCUUAGGGGCGCGCCGG 20 3096

HSV2-UL30-84 + CCCGGUGCGCUGCUCCGCGG 20 3097

HSV2-UL30-85 + GCGUCCUCGUCCAGCGAACG 20 3098

HSV2-UL30-86 + CGUCCUCGUCCAGCGAACGC 20 3099

HSV2-UL30-89 + UAGUACGUAUGGCGCUGAGC 20 3100

HSV2-UL30-91 + AUGGCGCUGAGCCGGCCCGA 20 3101

HSV2-UL30-93 + GAGGGCCUUUGGCUGCGUUC 20 3102

HSV2-UL30-94 + GCUGCGUUCCGGUCUGAGCG 20 3103

HSV2-UL30-95 + GCGUUCCGGUCUGAGCGAGG 20 3104

HSV2-UL30-97 + GUUCCGGUCUGAGCGAGGUG 20 3105

HSV2-UL30-100 + GGUUGUAGAAGUUCUGCCGG 20 3106

HSV2-UL30-101 + UAGAAGUUCUGCCGGCGGCA 20 3107

HSV2-UL30-102 + AAGUUCUGCCGGCGGCAAGG 20 3108

HSV2-UL30-104 + GGCAAGGCGGCGGUGCCGUC 20 3109

HSV2-UL30-105 + GCAAGGCGGCGGUGCCGUCU 20 3110

HSV2-UL30-110 + CCGUCUGGGUGGCUCCCCGG 20 3111

HSV2-UL30-141 - CACCGCCGCCUUGCCGC 17 3112

HSV2-UL30-143 - GACCGGAACGCAGCCAA 17 3113

HSV2-UL30-148 - CCCGCGUUCGCUGGACG 17 3114

HSV2-UL30-154 - UCCACGACGGCCGCCUC 17 3115

HSV2-UL30-156 - GCCCCUAAGGUGUACUG 17 3116

HSV2-UL30-157 - CCCCUAAGGUGUACUGC 17 3117

HSV2-UL30-159 - CCUAAGGUGUACUGCGG 17 3118

HSV2-UL30-160 - CUAAGGUGUACUGCGGG 17 3119 HSV2-UL30-161 - UAAGGUGUACUGCGGGG 17 3120

HSV2-UL30-162 - GCGCGACGUCCUCCGCG 17 3121

HSV2-UL30-163 - CGCGACGUCCUCCGCGU 17 3122

HSV2-UL30-166 - CUCCGCGUGGGCCCGGA 17 3123

HSV2-UL30-169 - GCGUCGCUUGCGCCUGU 17 3124

HSV2-UL30-170 - CGUCGCU UGCGCCUGUG 17 3125

HSV2-UL30-171 - CGCU UGCGCCUGUGGGG 17 3126

HSV2-UL30-173 - UGCGGACCAUGCCCCCG 17 3127

HSV2-UL30-174 - GCGGACCAUGCCCCCGA 17 3128

HSV2-UL30-176 - CCACGUGUACGACAUCC 17 3129

HSV2-UL30-183 + UGGAAGACGGUGACGGU 17 3130

HSV2-UL30-185 + GGUGGGGUCGAACCCCU 17 3131

HSV2-UL30-186 + GUGGGGUCGAACCCCUC 17 3132

HSV2-UL30-187 + UGGGGUCGAACCCCUCG 17 3133

HSV2-UL30-188 + GGGGUCGAACCCCUCGG 17 3134

HSV2-UL30-189 + CGAACCCCUCGGGGGCA 17 3135

HSV2-UL30-191 + CACAGGCGCAAGCGACG 17 3136

HSV2-UL30-192 + CGCGGCCAGAAGCCCUC 17 3137

HSV2-UL30-197 + CCCCGCAGUACACCUUA 17 3138

HSV2-UL30-198 + CCCGCAGUACACCUUAG 17 3139

HSV2-UL30-199 + ACACCUUAGGGGCGCGC 17 3140

HSV2-UL30-200 + CCUUAGGGGCGCGCCGG 17 3141

HSV2-UL30-207 + GGUGCGCUGCUCCGCGG 17 3142

HSV2-UL30-208 + UCCUCGUCCAGCGAACG 17 3143

HSV2-UL30-209 + CCUCGUCCAGCGAACGC 17 3144

HSV2-UL30-212 + UACGUAUGGCGCUGAGC 17 3145

HSV2-UL30-214 + GCGCUGAGCCGGCCCGA 17 3146

HSV2-UL30-216 + GGCCU UUGGCUGCGUUC 17 3147

HSV2-UL30-217 + GCGUUCCGGUCUGAGCG 17 3148

HSV2-UL30-218 + UUCCGGUCUGAGCGAGG 17 3149

HSV2-UL30-220 + CCGGUCUGAGCGAGGUG 17 3150

HSV2-UL30-223 + UGUAGAAGUUCUGCCGG 17 3151

HSV2-UL30-224 + AAGUUCUGCCGGCGGCA 17 3152

HSV2-UL30-225 + UUCUGCCGGCGGCAAGG 17 3153

HSV2-UL30-227 + AAGGCGGCGGUGCCGUC 17 3154

HSV2-UL30-228 + AGGCGGCGGUGCCGUCU 17 3155

HSV2-UL30-233 + UCUGGGUGGCUCCCCGG 17 3156

Table 2B provides exemplary targeting domains for knocking out the UL30 gene selected according to the second tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL30 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2B

HSV2-UL30-44 - GCGUGGGCCCGGAGGGCUUC 20 3188

HSV2-UL30-45 - GGCCGCGUCGCUUGCGCCUG 20 3189

HSV2-UL30-49 - CUUGCGCCUGUGGGGCGGUG 20 3190

HSV2-UL30-52 - GUGCGGACCAUGCCCCCGAG 20 3191

HSV2-UL30-54 - GUACGACAUCCUGGAGCACG 20 3192

HSV2-UL30-55 + ACGCGUGUUCCACGUGCUCC 20 3193

HSV2-UL30-56 + GCUCCAGGAUGUCGUACACG 20 3194

HSV2-UL30-57 + GAUGUCGUACACGUGGAAGA 20 3195

HSV2-UL30-58 + GUACACGUGGAAGACGGUGA 20 3196

HSV2-UL30-59 + CACGUGGAAGACGGUGACGG 20 3197

HSV2-UL30-61 + CGUGGAAGACGGUGACGGUG 20 3198

HSV2-UL30-67 + CAU G G U CCG CACCG CCCCAC 20 3199

HSV2-UL30-70 + GACGCGGCCAGAAGCCCUCC 20 3200

HSV2-UL30-71 + AGAAGCCCUCCGGGCCCACG 20 3201

HSV2-UL30-72 + AGCCCUCCGGGCCCACGCGG 20 3202

HSV2-UL30-73 + UCCCCCCCGCAGUACACCUU 20 3203

HSV2-UL30-78 + CCUUAGGGGCGCGCCGGAGG 20 3204

HSV2-UL30-79 + CGCGCCGGAGGCGGCCGUCG 20 3205

HSV2-UL30-80 + GAGGCGGCCGUCGUGGACCC 20 3206

HSV2-UL30-81 + GACCCCGGUGCGCUGCUCCG 20 3207

HSV2-UL30-82 + ACCCCGGUGCGCUGCUCCGC 20 3208

HSV2-UL30-83 + CCCCGGUGCGCUGCUCCGCG 20 3209

HSV2-UL30-87 + GUCCUCGUCCAGCGAACGCG 20 3210

HSV2-UL30-88 + CGCACUCGCUGUAGUACGUA 20 3211

HSV2-UL30-90 + UAUGGCGCUGAGCCGGCCCG 20 3212

HSV2-UL30-92 + UGAGCCGGCCCGAGGGCCUU 20 3213

HSV2-UL30-96 + CGU UCCGGUCUGAGCGAGGU 20 3214

HSV2-UL30-98 + UUCCGGUCUGAGCGAGGUGG 20 3215

HSV2-UL30-99 + GGGGGUUGUAGAAGUUCUGC 20 3216

HSV2-UL30-103 + UUCUGCCGGCGGCAAGGCGG 20 3217

HSV2-UL30-106 + AGGCGGCGGUGCCGUCUGGG 20 3218

HSV2-UL30-107 + GUGCCGUCUGGGUGGCUCCC 20 3219

HSV2-UL30-108 + UGCCGUCUGGGUGGCUCCCC 20 3220

HSV2-UL30-109 + GCCGUCUGGGUGGCUCCCCG 20 3221

HSV2-UL30-111 + GGGUGGCUCCCCGGGGGUUG 20 3222

HSV2-UL30-112 + GGUGGCUCCCCGGGGGUUGU 20 3223

HSV2-UL30-113 + GUGGCUCCCCGGGGGUUGUG 20 3224

HSV2-UL30-114 + UGGCUCCCCGGGGGUUGUGG 20 3225

HSV2-UL30-115 + GGCUCCCCGGGGGUUGUGGG 20 3226

HSV2-UL30-116 + CCAGACGCCGCCCGAGCCGC 20 3227

HSV2-UL30-117 + CCGAGCCGCCGGCUUCCCCC 20 3228

HSV2-UL30-118 + CGAGCCGCCGGCUUCCCCCC 20 3229

HSV2-UL30-119 + GAGCCGCCGGCU UCCCCCCG 20 3230

HSV2-UL30-120 + AGCCGCCGGCUUCCCCCCGG 20 3231

HSV2-UL30-121 + GGCUUCCCCCCGGGGGAAGC 20 3232

HSV2-UL30-122 + GCUUCCCCCCGGGGGAAGCC 20 3233 HSV2-UL30-123 + GGGGGAAGCCGGGCCGCCCG 20 3234

HSV2-UL30-124 - UUGUGCCGCGGGCGGCC 17 3235

HSV2-UL30-125 - GGCGGCCCGGCUUCCCC 17 3236

HSV2-UL30-126 - GCGGCCCGGCUUCCCCC 17 3237

HSV2-UL30-127 - CGGCCCGGCUUCCCCCG 17 3238

HSV2-UL30-128 - GGCCCGGCUUCCCCCGG 17 3239

HSV2-UL30-129 - GCCCGGCUUCCCCCGGG 17 3240

HSV2-UL30-130 - UUCCCCCGGGGGGAAGC 17 3241

HSV2-UL30-131 - CCCCGGGGGGAAGCCGG 17 3242

HSV2-UL30-132 - GGGGGAAGCCGGCGGCU 17 3243

HSV2-UL30-133 - GGGGAAGCCGGCGGCUC 17 3244

HSV2-UL30-134 - GAAGCCGGCGGCUCGGG 17 3245

HSV2-UL30-135 - GCGGCUCGGGCGGCGUC 17 3246

HSV2-UL30-136 - CGGCUCGGGCGGCGUCU 17 3247

HSV2-UL30-137 - UUGCCCCCCACAACCCC 17 3248

HSV2-UL30-138 - UGCCCCCCACAACCCCC 17 3249

HSV2-UL30-139 - GCCCCCCACAACCCCCG 17 3250

HSV2-UL30-140 - CCGGGGAGCCACCCAGA 17 3251

HSV2-UL30-142 - CCCCACCUCGCUCAGAC 17 3252

HSV2-UL30-144 - ACGCAGCCAAAGGCCCU 17 3253

HSV2-UL30-145 - CGCAGCCAAAGGCCCUC 17 3254

HSV2-UL30-146 - GCCAAAGGCCCUCGGGC 17 3255

HSV2-UL30-147 - UAUCGCCCCGCGUUCGC 17 3256

HSV2-UL30-149 - GGACGAGGACGCCCCCG 17 3257

HSV2-UL30-150 - CCCGCGGAGCAGCGCAC 17 3258

HSV2-UL30-151 - CCGCGGAGCAGCGCACC 17 3259

HSV2-UL30-152 - CGCGGAGCAGCGCACCG 17 3260

HSV2-UL30-153 - CGCACCGGGGUCCACGA 17 3261

HSV2-UL30-155 - CCUCCGGCGCGCCCCUA 17 3262

HSV2-UL30-158 - CCCUAAGGUGUACUGCG 17 3263

HSV2-UL30-164 - CGUCCUCCGCGUGGGCC 17 3264

HSV2-UL30-165 - CCUCCGCGUGGGCCCGG 17 3265

HSV2-UL30-167 - UGGGCCCGGAGGGCUUC 17 3266

HSV2-UL30-168 - CGCGUCGCUUGCGCCUG 17 3267

HSV2-UL30-172 - GCGCCUGUGGGGCGGUG 17 3268

HSV2-UL30-175 - CGGACCAUGCCCCCGAG 17 3269

HSV2-UL30-177 - CGACAUCCUGGAGCACG 17 3270

HSV2-UL30-178 + CGUGUUCCACGUGCUCC 17 3271

HSV2-UL30-179 + CCAG G AU G U CGUACACG 17 3272

HSV2-UL30-180 + GUCGUACACGUGGAAGA 17 3273

HSV2-UL30-181 + CACGUGGAAGACGGUGA 17 3274

HSV2-UL30-182 + GUGGAAGACGGUGACGG 17 3275

HSV2-UL30-184 + GGAAGACGGUGACGGUG 17 3276

HSV2-UL30-190 + GGUCCGCACCGCCCCAC 17 3277

HSV2-UL30-193 + GCGGCCAGAAGCCCUCC 17 3278

HSV2-UL30-194 + AGCCCUCCGGGCCCACG 17 3279 HSV2-UL30-195 + CCUCCGGGCCCACGCGG 17 3280

HSV2-UL30-196 + CCCCCGCAGUACACCUU 17 3281

HSV2-UL30-201 + UAGGGGCGCGCCGGAGG 17 3282

HSV2-UL30-202 + GCCGGAGGCGGCCGUCG 17 3283

HSV2-UL30-203 + GCGGCCGUCGUGGACCC 17 3284

HSV2-UL30-204 + CCCGGUGCGCUGCUCCG 17 3285

HSV2-UL30-205 + CCGGUGCGCUGCUCCGC 17 3286

HSV2-UL30-206 + CGGUGCGCUGCUCCGCG 17 3287

HSV2-UL30-210 + CUCGUCCAGCGAACGCG 17 3288

HSV2-UL30-211 + ACUCGCUGUAGUACGUA 17 3289

HSV2-UL30-213 + GGCGCUGAGCCGGCCCG 17 3290

HSV2-UL30-215 + GCCGGCCCGAGGGCCUU 17 3291

HSV2-UL30-219 + UCCGGUCUGAGCGAGGU 17 3292

HSV2-UL30-221 + CGGUCUGAGCGAGGUGG 17 3293

HSV2-UL30-222 + GGUUGUAGAAGUUCUGC 17 3294

HSV2-UL30-226 + UGCCGGCGGCAAGGCGG 17 3295

HSV2-UL30-229 + CGGCGGUGCCGUCUGGG 17 3296

HSV2-UL30-230 + CCGUCUGGGUGGCUCCC 17 3297

HSV2-UL30-231 + CGUCUGGGUGGCUCCCC 17 3298

HSV2-UL30-232 + GUCUGGGUGGCUCCCCG 17 3299

HSV2-UL30-234 + UGGCUCCCCGGGGGUUG 17 3300

HSV2-UL30-235 + GGCUCCCCGGGGGUUGU 17 3301

HSV2-UL30-236 + GCUCCCCGGGGGU UGUG 17 3302

HSV2-UL30-237 + CUCCCCGGGGGUUGUGG 17 3303

HSV2-UL30-238 + UCCCCGGGGGUUGUGGG 17 3304

HSV2-UL30-239 + GACGCCGCCCGAGCCGC 17 3305

HSV2-UL30-240 + AGCCGCCGGCUUCCCCC 17 3306

HSV2-UL30-241 + GCCGCCGGCUUCCCCCC 17 3307

HSV2-UL30-242 + CCGCCGGCUUCCCCCCG 17 3308

HSV2-UL30-243 + CGCCGGCUUCCCCCCGG 17 3309

HSV2-UL30-244 + UUCCCCCCGGGGGAAGC 17 3310

HSV2-UL30-245 + UCCCCCCGGGGGAAGCC 17 3311

HSV2-UL30-246 + GGAAGCCGGGCCGCCCG 17 3312

Table 2C provides exemplary targeting domains for knocking out the UL30 gene selected according to the third tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of the UL30 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2C

HSV2-UL30-478 - GGGGCGUCGACGCCACCACC 20 3348

HSV2-UL30-479 - CGCCACCACCCGGUUUAUCC 20 3349

HSV2-UL30-480 - CCGGUUUAUCCUGGACAACC 20 3350

HSV2-UL30-481 - CGGUUUAUCCUGGACAACCC 20 3351

HSV2-UL30-482 - GGUUUAUCCUGGACAACCCG 20 3352

HSV2-UL30-483 - AACCCGGGGUUUGUCACCUU 20 3353

HSV2-UL30-484 - CGGGGUUUGUCACCUUCGGC 20 3354

HSV2-UL30-485 - GGCUGGUACCGCCUCAAGCC 20 3355

HSV2-UL30-486 - UACCGCCUCAAGCCCGGCCG 20 3356

HSV2-UL30-487 - ACCGCCUCAAGCCCGGCCGC 20 3357

HSV2-UL30-488 - GCCCGGCCGCGGGAACGCGC 20 3358

HSV2-UL30-489 - GGCCCAACCGCGCCCCCCGA 20 3359

HSV2-UL30-490 - CCGCGCCCCCCGACGGCGUU 20 3360

HSV2-UL30-491 - CGACGUCGAGUUUAACUGCA 20 3361

HSV2-UL30-492 - CGUCGAGUUUAACUGCACGG 20 3362

HSV2-UL30-493 - UAACUGCACGGCGGACAACC 20 3363

HSV2-UL30-494 - GGCGGACAACCUGGCCGUCG 20 3364

HSV2-UL30-495 - GCGGACAACCUGGCCGUCGA 20 3365

HSV2-UL30-496 - CGGACAACCUGGCCGUCGAG 20 3366

HSV2-UL30-497 - GGACAACCUGGCCGUCGAGG 20 3367

HSV2-UL30-498 - GGGGGCCAUGUGUGACCUGC 20 3368

HSV2-UL30-499 - GUGCUUCGAUAUCGAAUGCA 20 3369

HSV2-UL30-500 - UUCGAUAUCGAAUGCAAGGC 20 3370

HSV2-UL30-501 - UCGAUAUCGAAUGCAAGGCC 20 3371

HSV2-UL30-502 - CGAUAUCGAAUGCAAGGCCG 20 3372

HSV2-UL30-503 - GAUAUCGAAUGCAAGGCCGG 20 3373

HSV2-UL30-504 - AUAUCGAAUGCAAGGCCGGG 20 3374

HSV2-UL30-505 - UAUCGAAUGCAAGGCCGGGG 20 3375

HSV2-UL30-506 - CGAAUGCAAGGCCGGGGGGG 20 3376

HSV2-UL30-507 - GGCCGGGGGGGAGGACGAGC 20 3377

HSV2-UL30-508 - GGAGGACGAGCUGGCCUUUC 20 3378

HSV2-UL30-509 - CGAGCUGGCCUUUCCGGUCG 20 3379

HSV2-UL30-510 - CUUUCCGGUCGCGGAACGCC 20 3380

HSV2-UL30-511 - CACAUCCUCCUGUUUUCGCU 20 3381

HSV2-UL30-512 - ACCUCAGCGAUCUCGCCUCC 20 3382

HSV2-UL30-513 - CCUCAGCGAUCUCGCCUCCA 20 3383

HSV2-UL30-514 - CU CAG CG AU CU CG CCU CCAG 20 3384

HSV2-UL30-515 - UCUCGCCUCCAGGGGCCUGC 20 3385

HSV2-UL30-516 - CCUGCCGGCCCCCGUCGUCC 20 3386

HSV2-UL30-517 - CAGCGAAUUCGAGAUGCUGC 20 3387

HSV2-UL30-518 - AUGACCUUCGUCAAGCAGUA 20 3388

HSV2-UL30-519 - UACGGCCCCGAGUUCGUGAC 20 3389

HSV2-UL30-520 - ACGGCCCCGAGUUCGUGACC 20 3390

HSV2-UL30-521 - ACAACAU CAU CAACU U CG AC 20 3391

HSV2-UL30-522 - CUUCGUCCUGACCAAGCUGA 20 3392

HSV2-UL30-523 - CAAGCUGACGGAGAUCUACA 20 3393 HSV2-UL30-524 - AUCUACAAGGUCCCGCUCGA 20 3394

HSV2-UL30-525 - UCUACAAGGUCCCGCUCGAC 20 3395

HSV2-UL30-526 - AAGGUCCCGCUCGACGGGUA 20 3396

HSV2-UL30-527 - AGGUCCCGCUCGACGGGUAC 20 3397

HSV2-UL30-528 - GACGGGUACGGGCGCAUGAA 20 3398

HSV2-UL30-529 - GGUACGGGCGCAUGAACGGC 20 3399

HSV2-UL30-530 - GUACGGGCGCAUGAACGGCC 20 3400

HSV2-UL30-531 - UACGGGCGCAUGAACGGCCG 20 3401

HSV2-UL30-532 - GCCGGGGUGUGUUCCGCGUG 20 3402

HSV2-UL30-533 - CCGGGGUGUGUUCCGCGUGU 20 3403

HSV2-UL30-534 - GUGUUCCGCGUGUGGGACAU 20 3404

HSV2-UL30-535 - UCAGAAGCGCAGCAAGAUCA 20 3405

HSV2-UL30-536 - CGCAGCAAGAUCAAGGUGAA 20 3406

HSV2-UL30-537 - GCAGCAAGAUCAAGGUGAAC 20 3407

HSV2-UL30-538 - CAAGAUCAAGGUGAACGGGA 20 3408

HSV2-UL30-539 - AUGGUGAACAUCGACAUGUA 20 3409

HSV2-UL30-540 - GUACGGCAUCAUCACCGACA 20 3410

HSV2-UL30-541 - CAAGCUGAACGCCGUCGCCG 20 3411

HSV2-UL30-542 - CGUCGCCGAGGCCGUCUUGA 20 3412

HSV2-UL30-543 - CGUCUUGAAGGACAAGAAGA 20 3413

HSV2-UL30-544 - AUCCCCGCCUACUACGCCUC 20 3414

HSV2-UL30-545 - UCCCCGCCUACUACGCCUCC 20 3415

HSV2-UL30-546 - GCCUCCGGGCCCGCGCAGCG 20 3416

HSV2-UL30-547 - CCUCCGGGCCCGCGCAGCGC 20 3417

HSV2-UL30-548 - CUCCGGGCCCGCGCAGCGCG 20 3418

HSV2-UL30-549 - CCCGCGCAGCGCGGGGUGAU 20 3419

HSV2-UL30-550 - GAUCGGCGAGUAUUGUGUGC 20 3420

HSV2-UL30-551 - UUGUGUGCAGGACUCGCUGC 20 3421

HSV2-UL30-552 - GUGCAGGACUCGCUGCUGGU 20 3422

HSV2-UL30-553 - UGCAGGACUCGCUGCUGGUC 20 3423

HSV2-UL30-554 - CUUCAAGUUUCUGCCGCACC 20 3424

HSV2-UL30-555 - GCUUUCCGCCGUCGCGCGCC 20 3425

HSV2-UL30-556 - UUCCGCCGUCGCGCGCCUGG 20 3426

HSV2-UL30-557 - UCCGCCGUCGCGCGCCUGGC 20 3427

HSV2-UL30-558 - AUCACCCGCACCAUCUACGA 20 3428

HSV2-UL30-559 - CACGUGCCUCCUGCGCCUUG 20 3429

HSV2-UL30-560 - ACGUGCCUCCUGCGCCUUGC 20 3430

HSV2-UL30-561 - CCUGCGCCUUGCGGGCCAGA 20 3431

HSV2-UL30-562 - CUGCGCCUUGCGGGCCAGAA 20 3432

HSV2-UL30-563 - CCAGAAGGGCUUCAUCCUGC 20 3433

HSV2-UL30-564 - CU UCAUCCUG CCG G ACACCC 20 3434

HSV2-UL30-565 - UUCAUCCUG CCG G ACACCCA 20 3435

HSV2-UL30-566 - UCAUCCUGCCGGACACCCAG 20 3436

HSV2-UL30-567 - UCCUGCCGGACACCCAGGGG 20 3437

HSV2-UL30-568 - CGGACACCCAGGGGCGGUUU 20 3438

HSV2-UL30-569 - GGACACCCAGGGGCGGUUUC 20 3439 HSV2-UL30-570 - GACACCCAGGGGCGGUUUCG 20 3440

HSV2-UL30-571 - GCGGUUUCGGGGCCUCGACA 20 3441

HSV2-UL30-572 - GUUUCGGGGCCUCGACAAGG 20 3442

HSV2-UL30-573 - CAAGGAGGCGCCCAAGCGCC 20 3443

HSV2-UL30-574 - CCAAGCGCCCGGCCGUGCCU 20 3444

HSV2-UL30-575 - CAAGCGCCCGGCCGUGCCUC 20 3445

HSV2-UL30-576 - AAGCGCCCGGCCGUGCCUCG 20 3446

HSV2-UL30-577 - AGCGCCCGGCCGUGCCUCGG 20 3447

HSV2-UL30-578 - GCGCCCGGCCGUGCCUCGGG 20 3448

HSV2-UL30-579 - CCGGCCGUGCCUCGGGGGGA 20 3449

HSV2-UL30-580 - CGGCCGUGCCUCGGGGGGAA 20 3450

HSV2-UL30-581 - GGCCGUGCCUCGGGGGGAAG 20 3451

HSV2-UL30-582 - UGCCUCGGGGGGAAGGGGAG 20 3452

HSV2-UL30-583 - UCGGGGGGAAGGGGAGCGGC 20 3453

HSV2-UL30-584 - CGGGGGGAAGGGGAGCGGCC 20 3454

HSV2-UL30-585 - GGGGGGAAGGGGAGCGGCCG 20 3455

HSV2-UL30-586 - GGGGGAAGGGGAGCGGCCGG 20 3456

HSV2-UL30-587 - GAAGGGGAGCGGCCGGGGGA 20 3457

HSV2-UL30-588 - AAGGGGAGCGGCCGGGGGAC 20 3458

HSV2-UL30-589 - GAGCGGCCGGGGGACGGGAA 20 3459

HSV2-UL30-590 - AGCGGCCGGGGGACGGGAAC 20 3460

HSV2-UL30-591 - GCGGCCGGGGGACGGGAACG 20 3461

HSV2-UL30-592 - GGGGGACGGGAACGGGGACG 20 3462

HSV2-UL30-593 - CGGGAACGGGGACGAGGAUA 20 3463

HSV2-UL30-594 - CGAGGAUAAGGACGACGACG 20 3464

HSV2-UL30-595 - GAUAAGGACGACGACGAGGA 20 3465

HSV2-UL30-596 - AUAAGGACGACGACGAGGAC 20 3466

HSV2-UL30-597 - UAAGGACGACGACGAGGACG 20 3467

HSV2-UL30-598 - CGACGACGAGGACGGGGACG 20 3468

HSV2-UL30-599 - GACGAGGACGGGGACGAGGA 20 3469

HSV2-UL30-600 - ACGAGGACGGGGACGAGGAC 20 3470

HSV2-UL30-601 - CGAGGACGGGGACGAGGACG 20 3471

HSV2-UL30-602 - CGAGGACGGGGACGAGCGCG 20 3472

HSV2-UL30-603 - GGACGGGGACGAGCGCGAGG 20 3473

HSV2-UL30-604 - GAGGAGGUCGCGCGCGAGAC 20 3474

HSV2-UL30-605 - AGGAGGUCGCGCGCGAGACC 20 3475

HSV2-UL30-606 - GGAGGUCGCGCGCGAGACCG 20 3476

HSV2-UL30-607 - GAGGUCGCGCGCGAGACCGG 20 3477

HSV2-UL30-608 - UCGCGCGCGAGACCGGGGGC 20 3478

HSV2-UL30-609 - GAGACCGGGGGCCGGCACGU 20 3479

HSV2-UL30-610 - AGACCGGGGGCCGGCACGUU 20 3480

HSV2-UL30-611 - GGGCCGGCACGUUGGGUACC 20 3481

HSV2-UL30-612 - GGCCGGCACGUUGGGUACCA 20 3482

HSV2-UL30-613 - GCCGGCACGU UGGGUACCAG 20 3483

HSV2-UL30-614 - CCGGCACGUUGGGUACCAGG 20 3484

HSV2-UL30-615 - ACGUUGGGUACCAGGGGGCC 20 3485 HSV2-UL30-616 - CGU UGGGUACCAGGGGGCCC 20 3486

HSV2-UL30-617 - CG G G U CCU CG ACCCCACCU C 20 3487

HSV2-UL30-618 - GGGUCCUCGACCCCACCUCC 20 3488

HSV2-UL30-619 - CGGGUUUCACGUCGACCCCG 20 3489

HSV2-UL30-620 - GUUUCACGUCGACCCCGUGG 20 3490

HSV2-UL30-621 - CCUGUACCCCAGCAUCAUCC 20 3491

HSV2-UL30-622 - GCUUCAGUACGCUCUCCCUG 20 3492

HSV2-UL30-623 - UACGCUCUCCCUGCGGCCCG 20 3493

HSV2-UL30-624 - GCCCGAGGCCGUCGCGCACC 20 3494

HSV2-UL30-625 - CGAGGCCGUCGCGCACCUGG 20 3495

HSV2-UL30-626 - GGCCGUCGCGCACCUGGAGG 20 3496

HSV2-UL30-627 - UCGCGCACCUGGAGGCGGAC 20 3497

HSV2-UL30-628 - CGCGCACCUGGAGGCGGACC 20 3498

HSV2-UL30-629 - GGAGGCGGACCGGGACUACC 20 3499

HSV2-UL30-630 - CCGGGACUACCUGGAGAUCG 20 3500

HSV2-UL30-631 - GGACUACCUGGAGAUCGAGG 20 3501

HSV2-UL30-632 - GACUACCUGGAGAUCGAGGU 20 3502

HSV2-UL30-633 - ACUACCUGGAGAUCGAGGUG 20 3503

HSV2-UL30-634 - CUACCUGGAGAUCGAGGUGG 20 3504

HSV2-UL30-635 - UACCUGGAGAUCGAGGUGGG 20 3505

HSV2-UL30-636 - AGAUCGAGGUGGGGGGCCGA 20 3506

HSV2-UL30-637 - CCGACGGCUGUUCUUCGUGA 20 3507

HSV2-UL30-638 - UGAGCAUCCUGCUGCGCGAC 20 3508

HSV2-UL30-639 - CAUCCUGCUGCGCGACUGGC 20 3509

HSV2-UL30-640 - UGCGAAAGCAGAUCCGCUCG 20 3510

HSV2-UL30-641 - G AU CCCCCAG AG CACCCCCG 20 3511

HSV2-UL30-642 - CCCCCAGAGCACCCCCGAGG 20 3512

HSV2-UL30-643 - CGUCCUCCUCG ACAAG CAAC 20 3513

HSV2-UL30-644 - CA AG CAACAG G CCG CCAU CA 20 3514

HSV2-UL30-645 - GCAACAGGCCGCCAUCAAGG 20 3515

HSV2-UL30-646 - CAUCAAGGUGGUGUGCAACU 20 3516

HSV2-UL30-647 - GUGGUGUGCAACUCGGUGUA 20 3517

HSV2-UL30-648 - UGGUGUGCAACUCGGUGUAC 20 3518

HSV2-UL30-649 - AACUCGGUGUACGGGUUCAC 20 3519

HSV2-UL30-650 - ACUCGGUGUACGGGUUCACC 20 3520

HSV2-UL30-651 - CUCGGUGUACGGGUUCACCG 20 3521

HSV2-UL30-652 - GGGUUCACCGGGGUGCAGCA 20 3522

HSV2-UL30-653 - UCUUCUGCCCUGCCUGCACG 20 3523

HSV2-UL30-654 - G CCG CCACCG U G ACG ACCAU 20 3524

HSV2-UL30-655 - GCGCGUACGUGCACGCGCGC 20 3525

HSV2-UL30-656 - CGCGUACGUGCACGCGCGCU 20 3526

HSV2-UL30-657 - GUACGUGCACGCGCGCUGGG 20 3527

HSV2-UL30-658 - GGCGGAGUUCGAUCAGCUGC 20 3528

HSV2-UL30-659 - UCAGCUGCUGGCCGACUUUC 20 3529

HSV2-UL30-660 - GCUGCUGGCCGACUUUCCGG 20 3530

HSV2-UL30-661 - GCUGGCCGACUUUCCGGAGG 20 3531 HSV2-UL30-662 - GCCGACUUUCCGGAGGCGGC 20 3532

HSV2-UL30-663 - GCGGCCGGCAUGCGCGCCCC 20 3533

HSV2-UL30-664 - UACUCCAUGCGCAUCAUCUA 20 3534

HSV2-UL30-665 - ACUCCAUGCGCAUCAUCUAC 20 3535

HSV2-UL30-666 - CUCCAUGCGCAUCAUCUACG 20 3536

HSV2-UL30-667 - GCGCAUCAUCUACGGGGACA 20 3537

HSV2-UL30-668 - UCCAUUUUCGUUUUGUGCCG 20 3538

HSV2-UL30-669 - CGUUUUGUGCCGCGGCCUCA 20 3539

HSV2-UL30-670 - GUGCCGCGGCCUCACGGCCG 20 3540

HSV2-UL30-671 - UGCCGCGGCCUCACGGCCGC 20 3541

HSV2-UL30-672 - CGGCCUCACGGCCGCGGGCC 20 3542

HSV2-UL30-673 - CCUCACGGCCGCGGGCCUGG 20 3543

HSV2-UL30-674 - GGCCGCGGGCCUGGUGGCCA 20 3544

HSV2-UL30-675 - GCCGCGGGCCUGGUGGCCAU 20 3545

HSV2-UL30-676 - GGUGGCCAUGGGCGACAAGA 20 3546

HSV2-UL30-677 - AU CG CCAAG AAAAAG U AC AU 20 3547

HSV2-UL30-678 - AAGUACAUCGGCGUCAUCUG 20 3548

HSV2-UL30-679 - AGUACAUCGGCGUCAUCUGC 20 3549

HSV2-UL30-680 - GUACAUCGGCGUCAUCUGCG 20 3550

HSV2-UL30-681 - UACAUCGGCGUCAUCUGCGG 20 3551

HSV2-UL30-682 - CGGGGGCAAGAUGCUCAUCA 20 3552

HSV2-UL30-683 - GGGGGCAAGAUGCUCAUCAA 20 3553

HSV2-UL30-684 - CAAGAUGCUCAUCAAGGGCG 20 3554

HSV2-UL30-685 - GCUCAUCAAGGGCGUGGAUC 20 3555

HSV2-UL30-686 - CGU UUAUCAACCGCACCUCC 20 3556

HSV2-UL30-687 - GUUUAUCAACCGCACCUCCA 20 3557

HSV2-UL30-688 - CAACCGCACCUCCAGGGCCC 20 3558

HSV2-UL30-689 - UUUUACGACGAUACCGUAUC 20 3559

HSV2-UL30-690 - CGACGAUACCGUAUCCGGAG 20 3560

HSV2-UL30-691 - GUUAGCCGAGCGCCCCGCAG 20 3561

HSV2-UL30-692 - CCGAGCGCCCCGCAGAGGAG 20 3562

HSV2-UL30-693 - GCGCCCCGCAGAGGAGUGGC 20 3563

HSV2-UL30-694 - GCUGGCGCGACCCCUGCCCG 20 3564

HSV2-UL30-695 - CUGGCGCGACCCCUGCCCGA 20 3565

HSV2-UL30-696 - ACCCCUGCCCGAGGGACUGC 20 3566

HSV2-UL30-697 - CCCGAGGGACUGCAGGCGUU 20 3567

HSV2-UL30-698 - CCGAGGGACUGCAGGCGUUC 20 3568

HSV2-UL30-699 - CGAGGGACUGCAGGCGUUCG 20 3569

HSV2-UL30-700 - CCGUCCUCGUAGACGCCCAU 20 3570

HSV2-UL30-701 - CCAUCGGCGCAUCACCGACC 20 3571

HSV2-UL30-702 - GGCGCAUCACCGACCCGGAG 20 3572

HSV2-UL30-703 - GCGCAUCACCGACCCGGAGA 20 3573

HSV2-UL30-704 - CGACCCGGAGAGGGACAUCC 20 3574

HSV2-UL30-705 - CG CG U ACACCAACAAG CG CC 20 3575

HSV2-UL30-706 - CAAGCGCCUGGCCCACCUGA 20 3576

HSV2-UL30-707 - GACGGUGUAUUACAAGCUCA 20 3577 HSV2-UL30-708 - GCUCAUGGCCCGCCGCGCGC 20 3578

HSV2-UL30-709 - CGCGCAGGUCCCGUCCAUCA 20 3579

HSV2-UL30-710 - AGGUCCCGUCCAU CAAG G AC 20 3580

HSV2-UL30-711 - CCGGAUCCCGUACGUGAUCG 20 3581

HSV2-UL30-712 - GAUCGUGGCCCAGACCCGCG 20 3582

HSV2-UL30-713 - GGCCCAGACCCGCGAGGUAG 20 3583

HSV2-UL30-714 - GACCCGCGAGGUAGAGGAGA 20 3584

HSV2-UL30-715 - AGGUAGAGGAGACGGUCGCG 20 3585

HSV2-UL30-716 - AGAGGAGACGGUCGCGCGGC 20 3586

HSV2-UL30-717 - GAGCUAGACGCCGCCGCCCC 20 3587

HSV2-UL30-718 - AGCUAGACGCCGCCGCCCCA 20 3588

HSV2-UL30-719 - GCUAGACGCCGCCGCCCCAG 20 3589

HSV2-UL30-720 - GGACGAGCCCGCCCCCCCAG 20 3590

HSV2-UL30-721 - CCCAGCGGCCCUGCCCUCCC 20 3591

HSV2-UL30-722 - CCUCCCCGGCCAAGCGCCCC 20 3592

HSV2-UL30-723 - CUCCCCGGCCAAGCGCCCCC 20 3593

HSV2-UL30-724 - G CCG U CG CAU GCCG ACCCCC 20 3594

HSV2-UL30-725 - CCGUCGCAUGCCGACCCCCC 20 3595

HSV2-UL30-726 - UCGCAUGCCGACCCCCCGGG 20 3596

HSV2-UL30-727 - GUCCAAGCCCCGCAAGCUGC 20 3597

HSV2-UL30-728 - CAAGCUGCUGGUGUCCGAGC 20 3598

HSV2-UL30-729 - GCUGCUGGUGUCCGAGCUGG 20 3599

HSV2-UL30-730 - GCUGGUGUCCGAGCUGGCGG 20 3600

HSV2-UL30-731 - UCCGAGCUGGCGGAGGAUCC 20 3601

HSV2-UL30-732 - CCGAGCUGGCGGAGGAUCCC 20 3602

HSV2-UL30-733 - AUCCCGGGUACGCCAUCGCC 20 3603

HSV2-UL30-734 - UCCCGGGUACGCCAUCGCCC 20 3604

HSV2-UL30-735 - CCCGGGUACGCCAUCGCCCG 20 3605

HSV2-UL30-736 - CCGGGGCGUUCCGCUCAACA 20 3606

HSV2-UL30-737 - CUAUUACUUCUCGCACCUGC 20 3607

HSV2-UL30-738 - UAUUACUUCUCGCACCUGCU 20 3608

HSV2-UL30-739 - AUUACUUCUCGCACCUGCUG 20 3609

HSV2-UL30-740 - UUACUUCUCGCACCUGCUGG 20 3610

HSV2-UL30-741 - CUUCUCGCACCUGCUGGGGG 20 3611

HSV2-UL30-742 - GGCGGCCUGCGUGACGUUCA 20 3612

HSV2-UL30-743 - GUGACGUUCAAGGCCCUGUU 20 3613

HSV2-UL30-744 - UCACCGAGAGUCUGUUAAAG 20 3614

HSV2-UL30-745 - AGAGGUUUAUUCCCGAGACG 20 3615

HSV2-UL30-746 - UCCCGAGACGUGGCACCCCC 20 3616

HSV2-UL30-747 - GUGGCACCCCCCGGACGACG 20 3617

HSV2-UL30-748 - CCCCGGACGACGUGGCCGCG 20 3618

HSV2-UL30-749 - ACGACGUGGCCGCGCGGCUC 20 3619

HSV2-UL30-750 - CGACGUGGCCGCGCGGCUCA 20 3620

HSV2-UL30-751 - GGCCGCGCGGCUCAGGGCCG 20 3621

HSV2-UL30-752 - GCCGCGCGGCUCAGGGCCGC 20 3622

HSV2-UL30-753 - CCGCGCGGCUCAGGGCCGCG 20 3623 HSV2-UL30-754 - CGGCUCAGGGCCGCGGGGUU 20 3624

HSV2-UL30-755 - GGCUCAGGGCCGCGGGGUUC 20 3625

HSV2-UL30-756 - CAGGGCCGCGGGGUUCGGGC 20 3626

HSV2-UL30-757 - GGCCGCGGGGUUCGGGCCGG 20 3627

HSV2-UL30-758 - GCCGCGGGGUUCGGGCCGGC 20 3628

HSV2-UL30-759 - CCGCGGGGUUCGGGCCGGCG 20 3629

HSV2-UL30-760 - CGCGGGGUUCGGGCCGGCGG 20 3630

HSV2-UL30-761 - GGGUUCGGGCCGGCGGGGGC 20 3631

HSV2-UL30-762 - GCCGGCGGGGGCCGGCGCUA 20 3632

HSV2-UL30-763 - GGCGGGGGCCGGCGCUACGG 20 3633

HSV2-UL30-764 - GGGGGCCGGCGCUACGGCGG 20 3634

HSV2-UL30-765 - GACGCCAUCACGCCCGC 17 3635

HSV2-UL30-766 - ACGCCAUCACGCCCGCC 17 3636

HSV2-UL30-767 - GACCGUCAUCACGCUUC 17 3637

HSV2-UL30-768 - ACCGUCAUCACGCUUCU 17 3638

HSV2-UL30-769 - CUGGGUCUGACCCCCGA 17 3639

HSV2-UL30-770 - GUCGCCGUUCACGUCUA 17 3640

HSV2-UL30-771 - UUCACGUCUACGGCACG 17 3641

HSV2-UL30-772 - GUACUUUUACAUGAACA 17 3642

HSV2-UL30-773 - CU U U U ACAU G AACAAGG 17 3643

HSV2-UL30-774 - UUACAUGAACAAGGCGG 17 3644

HSV2-UL30-775 - CAUGAACAAGGCGGAGG 17 3645

HSV2-UL30-776 - ACAAGGCGGAGGUGGAU 17 3646

HSV2-UL30-777 - CGAUCUCUGCGAGCGCC 17 3647

HSV2-UL30-778 - UCUCUGCGAGCGCCUGG 17 3648

HSV2-UL30-779 - CUGCGAGCGCCUGGCGG 17 3649

HSV2-UL30-780 - GGCCCUGCGCGAGUCGC 17 3650

HSV2-UL30-781 - GCCCUGCGCGAGUCGCC 17 3651

HSV2-UL30-782 - CCCUGCGCGAGUCGCCG 17 3652

HSV2-UL30-783 - CCUGCGCGAGUCGCCGG 17 3653

HSV2-UL30-784 - CCGGGGGCGUCGUUCCG 17 3654

HSV2-UL30-785 - GUUCCGCGGCAUCUCCG 17 3655

HSV2-UL30-786 - CU CCG CGG ACCACU U CG 17 3656

HSV2-UL30-787 - CGCGGACCACUUCGAGG 17 3657

HSV2-UL30-788 - GGACCACUUCGAGGCGG 17 3658

HSV2-UL30-789 - CCACUUCGAGGCGGAGG 17 3659

HSV2-UL30-790 - CU UCGAGGCGGAGGUGG 17 3660

HSV2-UL30-791 - CGCGUCUUCGUGCGAAG 17 3661

HSV2-UL30-792 - GCGUCUUCGUGCGAAGC 17 3662

HSV2-UL30-793 - GCGAAGCGGGCGCGCGC 17 3663

HSV2-UL30-794 - ACUUUUGCCCCGCGAUC 17 3664

HSV2-UL30-795 - CGCGAUCAGGAAGUACG 17 3665

HSV2-UL30-796 - GCGAUCAGGAAGUACGA 17 3666

HSV2-UL30-797 - CGAUCAGGAAGUACGAG 17 3667

HSV2-UL30-798 - GAUCAGGAAGUACGAGG 17 3668

HSV2-UL30-799 - AUCAGGAAGUACGAGGG 17 3669 HSV2-UL30-800 - G CG U CGACG CCACCACC 17 3670

HSV2-UL30-801 - CACCACCCG G U U U AU CC 17 3671

HSV2-UL30-802 - GUUUAUCCUGGACAACC 17 3672

HSV2-UL30-803 - UUUAUCCUGGACAACCC 17 3673

HSV2-UL30-804 - UUAUCCUGGACAACCCG 17 3674

HSV2-UL30-805 - CCGGGGUUUGUCACCUU 17 3675

HSV2-UL30-806 - GGUUUGUCACCUUCGGC 17 3676

HSV2-UL30-807 - UGGUACCGCCUCAAGCC 17 3677

HSV2-UL30-808 - CGCCUCAAGCCCGGCCG 17 3678

HSV2-UL30-809 - GCCUCAAGCCCGGCCGC 17 3679

HSV2-UL30-810 - CGGCCGCGGGAACGCGC 17 3680

HSV2-UL30-811 - CCAACCGCGCCCCCCGA 17 3681

HSV2-UL30-812 - CGCCCCCCGACGGCGUU 17 3682

HSV2-UL30-813 - CGUCGAGUUUAACUGCA 17 3683

HSV2-UL30-814 - CGAGUUUAACUGCACGG 17 3684

HSV2-UL30-815 - CUGCACGGCGGACAACC 17 3685

HSV2-UL30-816 - GGACAACCUGGCCGUCG 17 3686

HSV2-UL30-817 - GACAACCUGGCCGUCGA 17 3687

HSV2-UL30-818 - ACAACCUGGCCGUCGAG 17 3688

HSV2-UL30-819 - CAACCUGGCCGUCGAGG 17 3689

HSV2-UL30-820 - GGCCAUGUGUGACCUGC 17 3690

HSV2-UL30-821 - CUUCGAUAUCGAAUGCA 17 3691

HSV2-UL30-822 - GAUAUCGAAUGCAAGGC 17 3692

HSV2-UL30-823 - AUAUCGAAUGCAAGGCC 17 3693

HSV2-UL30-824 - UAUCGAAUGCAAGGCCG 17 3694

HSV2-UL30-825 - AUCGAAUGCAAGGCCGG 17 3695

HSV2-UL30-826 - UCGAAUGCAAGGCCGGG 17 3696

HSV2-UL30-827 - CGAAUGCAAGGCCGGGG 17 3697

HSV2-UL30-828 - AUGCAAGGCCGGGGGGG 17 3698

HSV2-UL30-829 - CGGGGGGGAGGACGAGC 17 3699

HSV2-UL30-830 - GGACGAGCUGGCCUUUC 17 3700

HSV2-UL30-831 - GCUGGCCUUUCCGGUCG 17 3701

HSV2-UL30-832 - UCCGGUCGCGGAACGCC 17 3702

HSV2-UL30-833 - AUCCUCCUGUUUUCGCU 17 3703

HSV2-UL30-834 - UCAGCGAUCUCGCCUCC 17 3704

HSV2-UL30-835 - CAGCGAUCUCGCCUCCA 17 3705

HSV2-UL30-836 - AGCGAUCUCGCCUCCAG 17 3706

HSV2-UL30-837 - CGCCUCCAGGGGCCUGC 17 3707

HSV2-UL30-838 - GCCGGCCCCCGUCGUCC 17 3708

HSV2-UL30-839 - CGAAUUCGAGAUGCUGC 17 3709

HSV2-UL30-840 - ACCUUCGUCAAGCAGUA 17 3710

HSV2-UL30-841 - GGCCCCGAGU UCGUGAC 17 3711

HSV2-UL30-842 - GCCCCGAGUUCGUGACC 17 3712

HSV2-UL30-843 - ACAUCAU CAACU U CG AC 17 3713

HSV2-UL30-844 - CG U CCU G ACCAAG CU G A 17 3714

HSV2-UL30-845 - GCUGACGGAGAUCUACA 17 3715 HSV2-UL30-846 - UACAAGGUCCCGCUCGA 17 3716

HSV2-UL30-847 - ACAAGGUCCCGCUCGAC 17 3717

HSV2-UL30-848 - GUCCCGCUCGACGGGUA 17 3718

HSV2-UL30-849 - UCCCGCUCGACGGGUAC 17 3719

HSV2-UL30-850 - GGGUACGGGCGCAUGAA 17 3720

HSV2-UL30-851 - ACGGGCGCAUGAACGGC 17 3721

HSV2-UL30-852 - CGGGCGCAUGAACGGCC 17 3722

HSV2-UL30-853 - GGGCGCAUGAACGGCCG 17 3723

HSV2-UL30-854 - GGGGUGUGUUCCGCGUG 17 3724

HSV2-UL30-855 - GGGUGUGUUCCGCGUGU 17 3725

HSV2-UL30-856 - UUCCGCGUGUGGGACAU 17 3726

HSV2-UL30-857 - GAAGCGCAGCAAGAUCA 17 3727

HSV2-UL30-858 - AGCAAGAUCAAGGUGAA 17 3728

HSV2-UL30-859 - GCAAGAUCAAGGUGAAC 17 3729

HSV2-UL30-860 - GAUCAAGGUGAACGGGA 17 3730

HSV2-UL30-861 - GUGAACAUCGACAUGUA 17 3731

HSV2-UL30-862 - CGGCAUCAUCACCGACA 17 3732

HSV2-UL30-863 - GCUGAACGCCGUCGCCG 17 3733

HSV2-UL30-864 - CGCCGAGGCCGUCUUGA 17 3734

HSV2-UL30-865 - CUUGAAGGACAAGAAGA 17 3735

HSV2-UL30-866 - CCCGCCUACUACGCCUC 17 3736

HSV2-UL30-867 - CCGCCUACUACGCCUCC 17 3737

HSV2-UL30-868 - UCCGGGCCCGCGCAGCG 17 3738

HSV2-UL30-869 - CCGGGCCCGCGCAGCGC 17 3739

HSV2-UL30-870 - CGGGCCCGCGCAGCGCG 17 3740

HSV2-UL30-871 - GCGCAGCGCGGGGUGAU 17 3741

HSV2-UL30-872 - CGGCGAGUAUUGUGUGC 17 3742

HSV2-UL30-873 - UGUGCAGGACUCGCUGC 17 3743

HSV2-UL30-874 - CAGGACUCGCUGCUGGU 17 3744

HSV2-UL30-875 - AGGACUCGCUGCUGGUC 17 3745

HSV2-UL30-876 - CAAGUUUCUGCCGCACC 17 3746

HSV2-UL30-877 - UUCCGCCGUCGCGCGCC 17 3747

HSV2-UL30-878 - CGCCGUCGCGCGCCUGG 17 3748

HSV2-UL30-879 - GCCGUCGCGCGCCUGGC 17 3749

HSV2-UL30-880 - ACCCGCACCAUCUACGA 17 3750

HSV2-UL30-881 - GUGCCUCCUGCGCCUUG 17 3751

HSV2-UL30-882 - UGCCUCCUGCGCCUUGC 17 3752

HSV2-UL30-883 - GCGCCUUGCGGGCCAGA 17 3753

HSV2-UL30-884 - CGCCUUGCGGGCCAGAA 17 3754

HSV2-UL30-885 - GAAGGGCUUCAUCCUGC 17 3755

HSV2-UL30-886 - CAUCCUGCCGGACACCC 17 3756

HSV2-UL30-887 - AUCCUGCCGGACACCCA 17 3757

HSV2-UL30-888 - UCCUGCCGGACACCCAG 17 3758

HSV2-UL30-889 - UGCCGGACACCCAGGGG 17 3759

HSV2-UL30-890 - ACACCCAGGGGCGGUUU 17 3760

HSV2-UL30-891 - CACCCAGGGGCGGUUUC 17 3761 HSV2-UL30-892 - ACCCAGGGGCGGUUUCG 17 3762

HSV2-UL30-893 - GUUUCGGGGCCUCGACA 17 3763

HSV2-UL30-894 - UCGGGGCCUCGACAAGG 17 3764

HSV2-UL30-895 - GGAGGCGCCCAAGCGCC 17 3765

HSV2-UL30-896 - AGCGCCCGGCCGUGCCU 17 3766

HSV2-UL30-897 - GCGCCCGGCCGUGCCUC 17 3767

HSV2-UL30-898 - CGCCCGGCCGUGCCUCG 17 3768

HSV2-UL30-899 - GCCCGGCCGUGCCUCGG 17 3769

HSV2-UL30-900 - CCCGGCCGUGCCUCGGG 17 3770

HSV2-UL30-901 - GCCGUGCCUCGGGGGGA 17 3771

HSV2-UL30-902 - CCGUGCCUCGGGGGGAA 17 3772

HSV2-UL30-903 - CGUGCCUCGGGGGGAAG 17 3773

HSV2-UL30-904 - CUCGGGGGGAAGGGGAG 17 3774

HSV2-UL30-905 - GGGGGAAGGGGAGCGGC 17 3775

HSV2-UL30-906 - GGGGAAGGGGAGCGGCC 17 3776

HSV2-UL30-907 - GGGAAGGGGAGCGGCCG 17 3777

HSV2-UL30-908 - GGAAGGGGAGCGGCCGG 17 3778

HSV2-UL30-909 - GGGGAGCGGCCGGGGGA 17 3779

HSV2-UL30-910 - GGGAGCGGCCGGGGGAC 17 3780

HSV2-UL30-911 - CGGCCGGGGGACGGGAA 17 3781

HSV2-UL30-912 - GGCCGGGGGACGGGAAC 17 3782

HSV2-UL30-913 - GCCGGGGGACGGGAACG 17 3783

HSV2-UL30-914 - GGACGGGAACGGGGACG 17 3784

HSV2-UL30-915 - GAACGGGGACGAGGAUA 17 3785

HSV2-UL30-916 - GGAUAAGGACGACGACG 17 3786

HSV2-UL30-917 - AAGGACGACGACGAGGA 17 3787

HSV2-UL30-918 - AGGACGACGACGAGGAC 17 3788

HSV2-UL30-919 - GGACGACGACGAGGACG 17 3789

HSV2-UL30-920 - CGACGAGGACGGGGACG 17 3790

HSV2-UL30-921 - GAGGACGGGGACGAGGA 17 3791

HSV2-UL30-922 - AGGACGGGGACGAGGAC 17 3792

HSV2-UL30-923 - GGACGGGGACGAGGACG 17 3793

HSV2-UL30-924 - GGACGGGGACGAGCGCG 17 3794

HSV2-UL30-925 - CGGGGACGAGCGCGAGG 17 3795

HSV2-UL30-926 - GAGGUCGCGCGCGAGAC 17 3796

HSV2-UL30-927 - AGGUCGCGCGCGAGACC 17 3797

HSV2-UL30-928 - GGUCGCGCGCGAGACCG 17 3798

HSV2-UL30-929 - GUCGCGCGCGAGACCGG 17 3799

HSV2-UL30-930 - CGCGCGAGACCGGGGGC 17 3800

HSV2-UL30-931 - ACCGGGGGCCGGCACGU 17 3801

HSV2-UL30-932 - CCGGGGGCCGGCACGU U 17 3802

HSV2-UL30-933 - CCGGCACGUUGGGUACC 17 3803

HSV2-UL30-934 - CGGCACGUUGGGUACCA 17 3804

HSV2-UL30-935 - GGCACGUUGGGUACCAG 17 3805

HSV2-UL30-936 - GCACGUUGGGUACCAGG 17 3806

HSV2-UL30-937 - UUGGGUACCAGGGGGCC 17 3807 HSV2-UL30-938 - UGGGUACCAGGGGGCCC 17 3808

HSV2-UL30-939 - GUCCUCGACCCCACCUC 17 3809

HSV2-UL30-940 - U CCU CG ACCCCACCU CC 17 3810

HSV2-UL30-941 - G U U U CACG U CG ACCCCG 17 3811

HSV2-UL30-942 - UCACGUCGACCCCGUGG 17 3812

HSV2-UL30-943 - GUACCCCAGCAUCAUCC 17 3813

HSV2-UL30-944 - UCAGUACGCUCUCCCUG 17 3814

HSV2-UL30-945 - GCUCUCCCUGCGGCCCG 17 3815

HSV2-UL30-946 - CGAGGCCGUCGCGCACC 17 3816

HSV2-UL30-947 - GGCCGUCGCGCACCUGG 17 3817

HSV2-UL30-948 - CGUCGCGCACCUGGAGG 17 3818

HSV2-UL30-949 - CGCACCUGGAGGCGGAC 17 3819

HSV2-UL30-950 - GCACCUGGAGGCGGACC 17 3820

HSV2-UL30-951 - GGCGGACCGGGACUACC 17 3821

HSV2-UL30-952 - GGACUACCUGGAGAUCG 17 3822

HSV2-UL30-953 - CUACCUGGAGAUCGAGG 17 3823

HSV2-UL30-954 - UACCUGGAGAUCGAGGU 17 3824

HSV2-UL30-955 - ACCUGGAGAUCGAGGUG 17 3825

HSV2-UL30-956 - CCUGGAGAUCGAGGUGG 17 3826

HSV2-UL30-957 - CUGGAGAUCGAGGUGGG 17 3827

HSV2-UL30-958 - UCGAGGUGGGGGGCCGA 17 3828

HSV2-UL30-959 - ACGGCUGUUCUUCGUGA 17 3829

HSV2-UL30-960 - GCAUCCUGCUGCGCGAC 17 3830

HSV2-UL30-961 - CCUGCUGCGCGACUGGC 17 3831

HSV2-UL30-962 - GAAAGCAGAUCCGCUCG 17 3832

HSV2-UL30-963 - CCCCCAGAGCACCCCCG 17 3833

HSV2-UL30-964 - CCAGAGCACCCCCGAGG 17 3834

HSV2-UL30-965 - CCUCCUCGACAAGCAAC 17 3835

HSV2-UL30-966 - GCAACAGGCCGCCAUCA 17 3836

HSV2-UL30-967 - ACAGGCCGCCAUCAAGG 17 3837

HSV2-UL30-968 - CAAGGUGGUGUGCAACU 17 3838

HSV2-UL30-969 - GUGUGCAACUCGGUGUA 17 3839

HSV2-UL30-970 - UGUGCAACUCGGUGUAC 17 3840

HSV2-UL30-971 - UCGGUGUACGGGUUCAC 17 3841

HSV2-UL30-972 - CGGUGUACGGGUUCACC 17 3842

HSV2-UL30-973 - GGUGUACGGGUUCACCG 17 3843

HSV2-UL30-974 - UUCACCGGGGUGCAGCA 17 3844

HSV2-UL30-975 - UCUGCCCUGCCUGCACG 17 3845

HSV2-UL30-976 - GCCACCGUGACGACCAU 17 3846

HSV2-UL30-977 - CGUACGUGCACGCGCGC 17 3847

HSV2-UL30-978 - GUACGUGCACGCGCGCU 17 3848

HSV2-UL30-979 - CGUGCACGCGCGCUGGG 17 3849

HSV2-UL30-980 - GGAGUUCGAUCAGCUGC 17 3850

HSV2-UL30-981 - GCUGCUGGCCGACUUUC 17 3851

HSV2-UL30-982 - GCUGGCCGACUUUCCGG 17 3852

HSV2-UL30-983 - GGCCGACUUUCCGGAGG 17 3853 HSV2-UL30-984 - GACUUUCCGGAGGCGGC 17 3854

HSV2-UL30-985 - GCCGGCAUGCGCGCCCC 17 3855

HSV2-UL30-986 - UCCAUGCGCAUCAUCUA 17 3856

HSV2-UL30-987 - CCAUGCGCAUCAUCUAC 17 3857

HSV2-UL30-988 - CAUGCGCAUCAUCUACG 17 3858

HSV2-UL30-989 - CAUCAUCUACGGGGACA 17 3859

HSV2-UL30-990 - AUUUUCGUUUUGUGCCG 17 3860

HSV2-UL30-991 - UUUGUGCCGCGGCCUCA 17 3861

HSV2-UL30-992 - CCGCGGCCUCACGGCCG 17 3862

HSV2-UL30-993 - CGCGGCCUCACGGCCGC 17 3863

HSV2-UL30-994 - CCUCACGGCCGCGGGCC 17 3864

HSV2-UL30-995 - CACGGCCGCGGGCCUGG 17 3865

HSV2-UL30-996 - CGCGGGCCUGGUGGCCA 17 3866

HSV2-UL30-997 - GCGGGCCUGGUGGCCAU 17 3867

HSV2-UL30-998 - GGCCAUGGGCGACAAGA 17 3868

HSV2-UL30-999 - G CCAAG AAAAAG U ACAU 17 3869

HSV2-UL30-1000 - UACAUCGGCGUCAUCUG 17 3870

HSV2-UL30-1001 - ACAUCGGCGUCAUCUGC 17 3871

HSV2-UL30-1002 - CAUCGGCGUCAUCUGCG 17 3872

HSV2-UL30-1003 - AUCGGCGUCAUCUGCGG 17 3873

HSV2-UL30-1004 - GGGCAAGAUGCUCAUCA 17 3874

HSV2-UL30-1005 - GGCAAGAUGCUCAUCAA 17 3875

HSV2-UL30-1006 - GAUGCUCAUCAAGGGCG 17 3876

HSV2-UL30-1007 - CAUCAAGGGCGUGGAUC 17 3877

HSV2-UL30-1008 - UUAUCAACCGCACCUCC 17 3878

HSV2-UL30-1009 - UAUCAACCGCACCUCCA 17 3879

HSV2-UL30-1010 - CCGCACCUCCAGGGCCC 17 3880

HSV2-UL30-1011 - UACGACGAUACCGUAUC 17 3881

HSV2-UL30-1012 - CGAUACCGUAUCCGGAG 17 3882

HSV2-UL30-1013 - AGCCGAGCGCCCCGCAG 17 3883

HSV2-UL30-1014 - AGCGCCCCGCAGAGGAG 17 3884

HSV2-UL30-1015 - CCCCGCAGAGGAGUGGC 17 3885

HSV2-UL30-1016 - GGCGCGACCCCUGCCCG 17 3886

HSV2-UL30-1017 - GCGCGACCCCUGCCCGA 17 3887

HSV2-UL30-1018 - CCUGCCCGAGGGACUGC 17 3888

HSV2-UL30-1019 - GAGGGACUGCAGGCGUU 17 3889

HSV2-UL30-1020 - AGGGACUGCAGGCGUUC 17 3890

HSV2-UL30-1021 - GGGACUGCAGGCGUUCG 17 3891

HSV2-UL30-1022 - UCCUCGUAGACGCCCAU 17 3892

HSV2-UL30-1023 - UCGGCGCAUCACCGACC 17 3893

HSV2-UL30-1024 - GCAUCACCGACCCGGAG 17 3894

HSV2-UL30-1025 - CAUCACCGACCCGGAGA 17 3895

HSV2-UL30-1026 - CCCGGAGAGGGACAUCC 17 3896

HSV2-UL30-1027 - GUACACCAACAAGCGCC 17 3897

HSV2-UL30-1028 - GCGCCUGGCCCACCUGA 17 3898

HSV2-UL30-1029 - GGUGUAUUACAAGCUCA 17 3899 HSV2-UL30-1030 - CAUGGCCCGCCGCGCGC 17 3900

HSV2-UL30-1031 - GCAGGUCCCGUCCAUCA 17 3901

HSV2-UL30-1032 - UCCCGUCCAUCAAGGAC 17 3902

HSV2-UL30-1033 - GAUCCCGUACGUGAUCG 17 3903

HSV2-UL30-1034 - CGUGGCCCAGACCCGCG 17 3904

HSV2-UL30-1035 - CCAG ACCCG CG AG G U AG 17 3905

HSV2-UL30-1036 - CCGCGAGGUAGAGGAGA 17 3906

HSV2-UL30-1037 - UAGAGGAGACGGUCGCG 17 3907

HSV2-UL30-1038 - GGAGACGGUCGCGCGGC 17 3908

HSV2-UL30-1039 - CUAGACGCCGCCGCCCC 17 3909

HSV2-UL30-1040 - UAGACGCCGCCGCCCCA 17 3910

HSV2-UL30-1041 - AGACGCCGCCGCCCCAG 17 3911

HSV2-UL30-1042 - CGAGCCCGCCCCCCCAG 17 3912

HSV2-UL30-1043 - AGCGGCCCUGCCCUCCC 17 3913

HSV2-UL30-1044 - CCCCGGCCAAGCGCCCC 17 3914

HSV2-UL30-1045 - CCCGGCCAAGCGCCCCC 17 3915

HSV2-UL30-1046 - GUCGCAUGCCGACCCCC 17 3916

HSV2-UL30-1047 - UCGCAUGCCGACCCCCC 17 3917

HSV2-UL30-1048 - CAUGCCGACCCCCCGGG 17 3918

HSV2-UL30-1049 - CAAGCCCCGCAAGCUGC 17 3919

HSV2-UL30-1050 - GCUGCUGGUGUCCGAGC 17 3920

HSV2-UL30-1051 - GCUGGUGUCCGAGCUGG 17 3921

HSV2-UL30-1052 - GGUGUCCGAGCUGGCGG 17 3922

HSV2-UL30-1053 - GAGCUGGCGGAGGAUCC 17 3923

HSV2-UL30-1054 - AGCUGGCGGAGGAUCCC 17 3924

HSV2-UL30-1055 - CCGGGUACGCCAUCGCC 17 3925

HSV2-UL30-1056 - CGGGUACGCCAUCGCCC 17 3926

HSV2-UL30-1057 - GGGUACGCCAUCGCCCG 17 3927

HSV2-UL30-1058 - GGGCGUUCCGCUCAACA 17 3928

HSV2-UL30-1059 - UUACUUCUCGCACCUGC 17 3929

HSV2-UL30-1060 - UACUUCUCGCACCUGCU 17 3930

HSV2-UL30-1061 - ACUUCUCGCACCUGCUG 17 3931

HSV2-UL30-1062 - CUUCUCGCACCUGCUGG 17 3932

HSV2-UL30-1063 - CUCGCACCUGCUGGGGG 17 3933

HSV2-UL30-1064 - GGCCUGCGUGACGUUCA 17 3934

HSV2-UL30-1065 - ACGUU CAAG GCCCUGUU 17 3935

HSV2-UL30-1066 - CCGAGAGUCUGUUAAAG 17 3936

HSV2-UL30-1067 - GGUUUAUUCCCGAGACG 17 3937

HSV2-UL30-1068 - CGAGACGUGGCACCCCC 17 3938

HSV2-UL30-1069 - GCACCCCCCGGACGACG 17 3939

HSV2-UL30-1070 - CGGACGACGUGGCCGCG 17 3940

HSV2-UL30-1071 - ACGUGGCCGCGCGGCUC 17 3941

HSV2-UL30-1072 - CGUGGCCGCGCGGCUCA 17 3942

HSV2-UL30-1073 - CGCGCGGCUCAGGGCCG 17 3943

HSV2-UL30-1074 - GCGCGGCUCAGGGCCGC 17 3944

HSV2-UL30-1075 - CGCGGCUCAGGGCCGCG 17 3945 HSV2-UL30-1076 - CUCAGGGCCGCGGGGUU 17 3946

HSV2-UL30-1077 - UCAGGGCCGCGGGGUUC 17 3947

HSV2-UL30-1078 - GGCCGCGGGGUUCGGGC 17 3948

HSV2-UL30-1079 - CGCGGGGUUCGGGCCGG 17 3949

HSV2-UL30-1080 - GCGGGGUUCGGGCCGGC 17 3950

HSV2-UL30-1081 - CGGGGUUCGGGCCGGCG 17 3951

HSV2-UL30-1082 - GGGGUUCGGGCCGGCGG 17 3952

HSV2-UL30-1083 - UUCGGGCCGGCGGGGGC 17 3953

HSV2-UL30-1084 - GGCGGGGGCCGGCGCUA 17 3954

HSV2-UL30-1085 - GGGGGCCGGCGCUACGG 17 3955

HSV2-UL30-1086 - GGCCGGCGCUACGGCGG 17 3956

HSV2-UL30-1087 + AGUUUCCUCCGCCGUAGCGC 20 3957

HSV2-UL30-1088 + GCCGUAGCGCCGGCCCCCGC 20 3958

HSV2-UL30-1089 + CCCCGCCGGCCCGAACCCCG 20 3959

HSV2-UL30-1090 + CCCCGCGGCCCUGAGCCGCG 20 3960

HSV2-UL30-1091 + AGCCGCGCGGCCACGUCGUC 20 3961

HSV2-UL30-1092 + GCCGCGCGGCCACGUCGUCC 20 3962

HSV2-UL30-1093 + CCGCGCGGCCACGUCGUCCG 20 3963

HSV2-UL30-1094 + CGCGCGGCCACGUCGUCCGG 20 3964

HSV2-UL30-1095 + GCGCGGCCACGUCGUCCGGG 20 3965

HSV2-UL30-1096 + GUCCGGGGGGUGCCACGUCU 20 3966

HSV2-UL30-1097 + UCCGGGGGGUGCCACGUCUC 20 3967

HSV2-UL30-1098 + AAACCUCU U UAACAG ACUCU 20 3968

HSV2-UL30-1099 + UAACAGACUCUCGGUGAUCU 20 3969

HSV2-UL30-1100 + UCUUGGCGUUAUUUCCAAAC 20 3970

HSV2-UL30-1101 + CUUGGCGUUAUUUCCAAACA 20 3971

HSV2-UL30-1102 + CAGGGCCUUGAACGUCACGC 20 3972

HSV2-UL30-1103 + UCACGCAGGCCGCCCCCAGC 20 3973

HSV2-UL30-1104 + AAGUAAUAGUCCGUGUUGAG 20 3974

HSV2-UL30-1105 + CCGUGUUGAGCGGAACGCCC 20 3975

HSV2-UL30-1106 + CGUGUUGAGCGGAACGCCCC 20 3976

HSV2-UL30-1107 + GAGCGGAACGCCCCGGGCGA 20 3977

HSV2-UL30-1108 + GCCCCGGGCGAUGGCGUACC 20 3978

HSV2-UL30-1109 + CCCCGGGCGAUGGCGUACCC 20 3979

HSV2-UL30-1110 + CCCGGGAUCCUCCGCCAGCU 20 3980

HSV2-UL30-1111 + GCUCGGACACCAGCAGCUUG 20 3981

HSV2-UL30-1112 + CUCGGACACCAGCAGCUUGC 20 3982

HSV2-UL30-1113 + UCGGACACCAGCAGCUUGCG 20 3983

HSV2-UL30-1114 + CACCAGCAGCUUGCGGGGCU 20 3984

HSV2-UL30-1115 + CGGGGCUUGGACGCGCCUCC 20 3985

HSV2-UL30-1116 + GGGGCUUGGACGCGCCUCCC 20 3986

HSV2-UL30-1117 + GGGCUUGGACGCGCCUCCCG 20 3987

HSV2-UL30-1118 + GGCUUGGACGCGCCUCCCGG 20 3988

HSV2-UL30-1119 + GCUUGGACGCGCCUCCCGGG 20 3989

HSV2-UL30-1120 + GGACGCGCCUCCCGGGGGGU 20 3990

HSV2-UL30-1121 + CCCGGGGGGUCGGCAUGCGA 20 3991 HSV2-UL30-1122 + CGGCAUGCGACGGCGUCUCC 20 3992

HSV2-UL30-1123 + GGCAUGCGACGGCGUCUCCC 20 3993

HSV2-UL30-1124 + GCAUGCGACGGCGUCUCCCG 20 3994

HSV2-UL30-1125 + CAUGCGACGGCGUCUCCCGG 20 3995

HSV2-UL30-1126 + CGGCGUCUCCCGGGGGCGCU 20 3996

HSV2-UL30-1127 + GUCUCCCGGGGGCGCUUGGC 20 3997

HSV2-UL30-1128 + UCUCCCGGGGGCGCUUGGCC 20 3998

HSV2-UL30-1129 + CUCCCGGGGGCGCU UGGCCG 20 3999

HSV2-UL30-1130 + CCGGGGGCGCUUGGCCGGGG 20 4000

HSV2-UL30-1131 + CGGGGGCGCUUGGCCGGGGA 20 4001

HSV2-UL30-1132 + GGCGCU UGGCCGGGGAGGGC 20 4002

HSV2-UL30-1133 + GCGCUUGGCCGGGGAGGGCA 20 4003

HSV2-UL30-1134 + GCCGGGGAGGGCAGGGCCGC 20 4004

HSV2-UL30-1135 + CCGGGGAGGGCAGGGCCGCU 20 4005

HSV2-UL30-1136 + CGGGGAGGGCAGGGCCGCUG 20 4006

HSV2-UL30-1137 + GGGGAGGGCAGGGCCGCUGG 20 4007

HSV2-UL30-1138 + GGGAGGGCAGGGCCGCUGGG 20 4008

HSV2-UL30-1139 + GGAGGGCAGGGCCGCUGGGG 20 4009

HSV2-UL30-1140 + GGGCAGGGCCGCUGGGGGGG 20 4010

HSV2-UL30-1141 + GGCAGGGCCGCUGGGGGGGC 20 4011

HSV2-UL30-1142 + GGGGGGGCGGGCUCGUCCCC 20 4012

HSV2-UL30-1143 + GGGGGGCGGGCUCGUCCCCU 20 4013

HSV2-UL30-1144 + GGGGGCGGGCUCGUCCCCUG 20 4014

HSV2-UL30-1145 + GGCGGGCUCGUCCCCUGGGG 20 4015

HSV2-UL30-1146 + GGGCUCGUCCCCUGGGGCGG 20 4016

HSV2-UL30-1147 + GGGCGGCGGCGUCUAGCUCG 20 4017

HSV2-UL30-1148 + CGGCGGCGUCUAGCUCGCGG 20 4018

HSV2-UL30-1149 + GGCGGCGUCUAGCUCGCGGA 20 4019

HSV2-UL30-1150 + GGCGUCUAGCUCGCGGAGGG 20 4020

HSV2-UL30-1151 + CGACCGUCUCCUCUACCUCG 20 4021

HSV2-UL30-1152 + GACCGUCUCCUCUACCUCGC 20 4022

HSV2-UL30-1153 + UCUCCUCUACCUCGCGGGUC 20 4023

HSV2-UL30-1154 + CUCCUCUACCUCGCGGGUCU 20 4024

HSV2-UL30-1155 + GUCUGGGCCACGAUCACGUA 20 4025

HSV2-UL30-1156 + UCUGGGCCACGAUCACGUAC 20 4026

HSV2-UL30-1157 + CCACGAUCACGUACGGGAUC 20 4027

HSV2-UL30-1158 + GUACGGGAUCCGGUCCUUGA 20 4028

HSV2-UL30-1159 + GGGAUCCGGUCCUUGAUGGA 20 4029

HSV2-UL30-1160 + GGAUCCGGUCCUUGAUGGAC 20 4030

HSV2-UL30-1161 + UGAUGGACGGGACCUGCGCG 20 4031

HSV2-UL30-1162 + UGGACGGGACCUGCGCGCGG 20 4032

HSV2-UL30-1163 + GGACGGGACCUGCGCGCGGC 20 4033

HSV2-UL30-1164 + UGAGCUUGUAAUACACCGUC 20 4034

HSV2-UL30-1165 + GCU UGUAAUACACCGUCAGG 20 4035

HSV2-UL30-1166 + CUUGUAAUACACCGUCAGGU 20 4036

HSV2-UL30-1167 + AAUACACCGUCAGGUGGGCC 20 4037 HSV2-UL30-1168 + CAGGUGGGCCAGGCGCUUGU 20 4038

HSV2-UL30-1169 + CGCUUGUUGGUGUACGCGCG 20 4039

HSV2-UL30-1170 + GCUUGUUGGUGUACGCGCGC 20 4040

HSV2-UL30-1171 + GCGCGGGUGUCUGCUCAGUU 20 4041

HSV2-UL30-1172 + CGGGUGUCUGCUCAGUUCGG 20 4042

HSV2-UL30-1173 + GUCUGCUCAGUUCGGCGGUG 20 4043

HSV2-UL30-1174 + CGGCGGUGAGGACAAAGUCC 20 4044

HSV2-UL30-1175 + AAGUCCUGGAUGUCCCUCUC 20 4045

HSV2-UL30-1176 + AGUCCUGGAUGUCCCUCUCC 20 4046

HSV2-UL30-1177 + CUGGAUGUCCCUCUCCGGGU 20 4047

HSV2-UL30-1178 + CCGGGUCGGUGAUGCGCCGA 20 4048

HSV2-UL30-1179 + CGGGUCGGUGAUGCGCCGAU 20 4049

HSV2-UL30-1180 + UGCGCCGAUGGGCGUCUACG 20 4050

HSV2-UL30-1181 + CCGAUGGGCGUCUACGAGGA 20 4051

HSV2-UL30-1182 + CCCG AACGCCU G CAG U CCCU 20 4052

HSV2-UL30-1183 + CCGAACGCCUGCAGUCCCUC 20 4053

HSV2-UL30-1184 + ACGCCUGCAGUCCCUCGGGC 20 4054

HSV2-UL30-1185 + CGCCUGCAGUCCCUCGGGCA 20 4055

HSV2-UL30-1186 + GCCUGCAGUCCCUCGGGCAG 20 4056

HSV2-UL30-1187 + UCGCGCCAGCCACUCCUCUG 20 4057

HSV2-UL30-1188 + CGCGCCAGCCACUCCUCUGC 20 4058

HSV2-UL30-1189 + GCG CCAG CCACU CCU CU G CG 20 4059

HSV2-UL30-1190 + CCACUCCUCUGCGGGGCGCU 20 4060

HSV2-UL30-1191 + UGCGGGGCGCUCGGCUAACG 20 4061

HSV2-UL30-1192 + GGGGCGCUCGGCUAACGCGG 20 4062

HSV2-UL30-1193 + GGCUAACGCGGCGGCCGCUC 20 4063

HSV2-UL30-1194 + CGCGGCGGCCGCUCCGGAUA 20 4064

HSV2-UL30-1195 + CG G U AU CG U CG U AAAACAG C 20 4065

HSV2-UL30-1196 + CGUAAAACAGCAGGUCGACC 20 4066

HSV2-UL30-1197 + G U AAAACAGCAG G U CG ACCA 20 4067

HSV2-UL30-1198 + CAGCAGGUCGACCAGGGCCC 20 4068

HSV2-UL30-1199 + CAGGUCGACCAGGGCCCUGG 20 4069

HSV2-UL30-1200 + CGACCAGGGCCCUGGAGGUG 20 4070

HSV2-UL30-1201 + GACGCCGAUGUACUUUUUCU 20 4071

HSV2-UL30-1202 + CUUGGCGAUGAGCAGCAGCU 20 4072

HSV2-UL30-1203 + UUUUCGCACUCGAGCUUGAU 20 4073

HSV2-UL30-1204 + UUUCGCACUCGAGCUUGAUC 20 4074

HSV2-UL30-1205 + UUCGCACUCGAGCUUGAUCG 20 4075

HSV2-UL30-1206 + UCGCACUCGAGCUUGAUCGG 20 4076

HSV2-UL30-1207 + CGCACUCGAGCUUGAUCGGG 20 4077

HSV2-UL30-1208 + ACUCGAGCUUGAUCGGGGGG 20 4078

HSV2-UL30-1209 + GGAACAGCGCGCGCGAGAUG 20 4079

HSV2-UL30-1210 + GCUCGCCAUCUUGUCGCCCA 20 4080

HSV2-UL30-1211 + UCUUGUCGCCCAUGGCCACC 20 4081

HSV2-UL30-1212 + GCCCAUGGCCACCAGGCCCG 20 4082

HSV2-UL30-1213 + CCACCAGGCCCGCGGCCGUG 20 4083 HSV2-UL30-1214 + GGCCCGCGGCCGUGAGGCCG 20 4084

HSV2-UL30-1215 + G CCG CG G CACAAAACG AAAA 20 4085

HSV2-UL30-1216 + GUCCCCGUAGAUGAUGCGCA 20 4086

HSV2-UL30-1217 + UAGAUGAUGCGCAUGGAGUA 20 4087

HSV2-UL30-1218 + GAUGCGCAUGGAGUACGGAC 20 4088

HSV2-UL30-1219 + AUGCGCAUGGAGUACGGACC 20 4089

HSV2-UL30-1220 + UGCGCAUGGAGUACGGACCG 20 4090

HSV2-UL30-1221 + GCGCAUGGAGUACGGACCGG 20 4091

HSV2-UL30-1222 + CGGACCGGGGGCGCGCAUGC 20 4092

HSV2-UL30-1223 + GCGCGCAUGCCGGCCGCCUC 20 4093

HSV2-UL30-1224 + GCCGGCCGCCUCCGGAAAGU 20 4094

HSV2-UL30-1225 + GCACGUACGCGCGCGUCGCG 20 4095

HSV2-UL30-1226 + GCGUCGCGAGGAGCAUCUCG 20 4096

HSV2-UL30-1227 + GAGGAGCAUCUCGCGGCCGA 20 4097

HSV2-UL30-1228 + CUCGCGGCCGAUGGUCGUCA 20 4098

HSV2-UL30-1229 + GCGGCCGAUGGUCGUCACGG 20 4099

HSV2-UL30-1230 + GCCGAUGGUCGUCACGGUGG 20 4100

HSV2-UL30-1231 + UCACGGUGGCGGCCACGUGC 20 4101

HSV2-UL30-1232 + GGUGGCGGCCACGUGCAGGC 20 4102

HSV2-UL30-1233 + GUGGCGGCCACGUGCAGGCA 20 4103

HSV2-UL30-1234 + CAGAAGACCGUGCUGCACCC 20 4104

HSV2-UL30-1235 + CGAGUUGCACACCACCUUGA 20 4105

HSV2-UL30-1236 + G U U G CACACCACCU U G AU GG 20 4106

HSV2-UL30-1237 + UGGCGGCCUGUUGCUUGUCG 20 4107

HSV2-UL30-1238 + CGGCCUGUUGCUUGUCGAGG 20 4108

HSV2-UL30-1239 + CUGUUGCUUGUCGAGGAGGA 20 4109

HSV2-UL30-1240 + GUCGAGGAGGACGGCCUCCU 20 4110

HSV2-UL30-1241 + UCGAGGAGGACGGCCUCCUC 20 4111

HSV2-UL30-1242 + CGAGGAGGACGGCCUCCUCG 20 4112

HSV2-UL30-1243 + GAGGAGGACGGCCUCCUCGG 20 4113

HSV2-UL30-1244 + CGGCCUCCUCGGGGGUGCUC 20 4114

HSV2-UL30-1245 + GGCCUCCUCGGGGGUGCUCU 20 4115

HSV2-UL30-1246 + GCCUCCUCGGGGGUGCUCUG 20 4116

HSV2-UL30-1247 + CCUCCUCGGGGGUGCUCUGG 20 4117

HSV2-UL30-1248 + UGCUCUGGGGGAUCCGCGAG 20 4118

HSV2-UL30-1249 + CGAGCGGAUCUGCUUUCGCA 20 4119

HSV2-UL30-1250 + UGGCCAGCCAGUCGCGCAGC 20 4120

HSV2-UL30-1251 + CGCGCAGCAGGAUGCUCAGC 20 4121

HSV2-UL30-1252 + UCAGCAGGCUCUCGCGUACG 20 4122

HSV2-UL30-1253 + CAGCAGGCUCUCGCGUACGU 20 4123

HSV2-UL30-1254 + CCU U CACG AAG AACAG CCG U 20 4124

HSV2-UL30-1255 + GGCCCCCCACCUCGAUCUCC 20 4125

HSV2-UL30-1256 + CCUCGAUCUCCAGGUAGUCC 20 4126

HSV2-UL30-1257 + GGUAGUCCCGGUCCGCCUCC 20 4127

HSV2-UL30-1258 + GUCCGCCUCCAGGUGCGCGA 20 4128

HSV2-UL30-1259 + CUCCAGGUGCGCGACGGCCU 20 4129 HSV2-UL30-1260 + UCCAGGUGCGCGACGGCCUC 20 4130

HSV2-UL30-1261 + GCGCGACGGCCUCGGGCCGC 20 4131

HSV2-UL30-1262 + CGCGACGGCCUCGGGCCGCA 20 4132

HSV2-UL30-1263 + GGGAGAGCGUACUGAAGCAC 20 4133

HSV2-UL30-1264 + GCGUACUGAAGCACAGGUUG 20 4134

HSV2-UL30-1265 + CGUACUGAAGCACAGGUUGU 20 4135

HSV2-UL30-1266 + UGAAGCACAGGUUGUGGGCC 20 4136

HSV2-UL30-1267 + GUUGUGGGCCUGGAUGAUGC 20 4137

HSV2-UL30-1268 + UUGUGGGCCUGGAUGAUGCU 20 4138

HSV2-UL30-1269 + UGUGGGCCUGGAUGAUGCUG 20 4139

HSV2-UL30-1270 + CCUGGAUGAUGCUGGGGUAC 20 4140

HSV2-UL30-1271 + GAUGAUGCUGGGGUACAGGC 20 4141

HSV2-UL30-1272 + G G C AAAG U C AAAC ACCACCA 20 4142

HSV2-UL30-1273 + G CAAAG U CAAAC ACCACCAC 20 4143

HSV2-UL30-1274 + CAAAG U CAAACACCACCACG 20 4144

HSV2-UL30-1275 + CACGGGGUCGACGUG AAACC 20 4145

HSV2-UL30-1276 + GGGGUCGACGUGAAACCCGG 20 4146

HSV2-UL30-1277 + G U CGACG U G AAACCCG G AGG 20 4147

HSV2-UL30-1278 + UCGACGUGAAACCCGGAGGU 20 4148

HSV2-UL30-1279 + CGACG UGAAACCCGGAGGUG 20 4149

HSV2-UL30-1280 + GAAACCCGGAGGUGGGGUCG 20 4150

HSV2-UL30-1281 + CGGAGGUGGGGUCGAGGACC 20 4151

HSV2-UL30-1282 + GGAGGUGGGGUCGAGGACCC 20 4152

HSV2-UL30-1283 + GGUCGAGGACCCGGGCCCCC 20 4153

HSV2-UL30-1284 + CCCCCUGGUACCCAACGUGC 20 4154

HSV2-UL30-1285 + GUACCCAACGUGCCGGCCCC 20 4155

HSV2-UL30-1286 + UCGUCCCCGUUCCCGUCCCC 20 4156

HSV2-UL30-1287 + G GCCG CU CCCCU U CCCCCCG 20 4157

HSV2-UL30-1288 + CUCCCCUUCCCCCCGAGGCA 20 4158

HSV2-UL30-1289 + CCUUCCCCCCGAGGCACGGC 20 4159

HSV2-UL30-1290 + CUUCCCCCCGAGGCACGGCC 20 4160

HSV2-UL30-1291 + CCGAGGCACGGCCGGGCGCU 20 4161

HSV2-UL30-1292 + CGAGGCACGGCCGGGCGCUU 20 4162

HSV2-UL30-1293 + GCU UGGGCGCCUCCUUGUCG 20 4163

HSV2-UL30-1294 + CGAGGCCCCGAAACCGCCCC 20 4164

HSV2-UL30-1295 + GAGGCCCCGAAACCGCCCCU 20 4165

HSV2-UL30-1296 + CGAAACCGCCCCUGGGUGUC 20 4166

HSV2-UL30-1297 + ACCGCCCCUGGGUGUCCGGC 20 4167

HSV2-UL30-1298 + CCGGCAGGAUGAAGCCCUUC 20 4168

HSV2-UL30-1299 + UGAAGCCCUUCUGGCCCGCA 20 4169

HSV2-UL30-1300 + CCUUCUGGCCCGCAAGGCGC 20 4170

HSV2-UL30-1301 + UCUGGCCCGCAAGGCGCAGG 20 4171

HSV2-UL30-1302 + GCAGGAGGCACGUGAAGACG 20 4172

HSV2-UL30-1303 + ACGUGAAGACGCGGAUCUGC 20 4173

HSV2-UL30-1304 + GAUCUGCUGGCCGUCGUAGA 20 4174

HSV2-UL30-1305 + GCUGGCCGUCGUAGAUGGUG 20 4175 HSV2-UL30-1306 + CUGGCCGUCGUAGAUGGUGC 20 4176

HSV2-UL30-1307 + GGGUGAUGUUGAUGCCCGCC 20 4177

HSV2-UL30-1308 + GAUGCCCGCCAGGCGCGCGA 20 4178

HSV2-UL30-1309 + GCCCGCCAGGCGCGCGACGG 20 4179

HSV2-UL30-1310 + GCGCGACGGCGGAAAGCUCC 20 4180

HSV2-UL30-1311 + ACGGCGGAAAGCUCCAGGUG 20 4181

HSV2-UL30-1312 + GCCGAUCACCCCGCGCUGCG 20 4182

HSV2-UL30-1313 + CCGAUCACCCCGCGCUGCGC 20 4183

HSV2-UL30-1314 + CACCCCGCGCUGCGCGGGCC 20 4184

HSV2-UL30-1315 + CCCGCGCUGCGCGGGCCCGG 20 4185

HSV2-UL30-1316 + CGCGGGCCCGGAGGCGUAGU 20 4186

HSV2-UL30-1317 + GGGCCCGGAGGCGUAGUAGG 20 4187

HSV2-UL30-1318 + GGCCCGGAGGCGUAGUAGGC 20 4188

HSV2-UL30-1319 + GCCCGGAGGCGUAGUAGGCG 20 4189

HSV2-UL30-1320 + CGUAGUAGGCGGGGAUGUCG 20 4190

HSV2-UL30-1321 + CUUCUUCUUGUCCUUCAAGA 20 4191

HSV2-UL30-1322 + CU UGUCCUUCAAGACGGCCU 20 4192

HSV2-UL30-1323 + CU UCAAGACGGCCUCGGCGA 20 4193

HSV2-UL30-1324 + GACGGCGUUCAGCUUGUAGC 20 4194

HSV2-UL30-1325 + GCUGGAGAGUUUGACCUUGU 20 4195

HSV2-UL30-1326 + UCUUGCUGCGCUUCUGAAAG 20 4196

HSV2-UL30-1327 + UGCGCUUCUGAAAGUGGCUC 20 4197

HSV2-UL30-1328 + UCUGGCCGAUGUCCCACACG 20 4198

HSV2-UL30-1329 + CCCACACGCGGAACACACCC 20 4199

HSV2-UL30-1330 + AUGCGCCCGUACCCGUCGAG 20 4200

HSV2-UL30-1331 + UGCGCCCGUACCCGUCGAGC 20 4201

HSV2-UL30-1332 + CUUGUAGAUCUCCGUCAGCU 20 4202

HSV2-UL30-1333 + AGAUCUCCGUCAGCUUGGUC 20 4203

HSV2-UL30-1334 + CGUCAGCUUGGUCAGGACGA 20 4204

HSV2-UL30-1335 + GUCAGCUUGGUCAGGACGAA 20 4205

HSV2-UL30-1336 + GAAGUUGAUGAUGUUGUACC 20 4206

HSV2-UL30-1337 + GUUGUACCCGGUCACGAACU 20 4207

HSV2-UL30-1338 + UUGUACCCGGUCACGAACUC 20 4208

HSV2-UL30-1339 + UGUACCCGGUCACGAACUCG 20 4209

HSV2-UL30-1340 + GGGGCCGUACUGCUUGACGA 20 4210

HSV2-UL30-1341 + CUGCUUGACGAAGGUCAUGA 20 4211

HSV2-UL30-1342 + CGAAUUCGCUGUCAAACUCC 20 4212

HSV2-UL30-1343 + GCUGUCAAACUCCAGGACGA 20 4213

HSV2-UL30-1344 + CUGUCAAACUCCAGGACGAC 20 4214

HSV2-UL30-1345 + UGUCAAACUCCAGGACGACG 20 4215

HSV2-UL30-1346 + GUCAAACUCCAGGACGACGG 20 4216

HSV2-UL30-1347 + AACUCCAGGACGACGGGGGC 20 4217

HSV2-UL30-1348 + CCAGGACGACGGGGGCCGGC 20 4218

HSV2-UL30-1349 + GACGGGGGCCGGCAGGCCCC 20 4219

HSV2-UL30-1350 + GGGGGCCGGCAGGCCCCUGG 20 4220

HSV2-UL30-1351 + CCCUGGAGGCGAGAUCGCUG 20 4221 HSV2-UL30-1352 + UGGAGGCGAGAUCGCUGAGG 20 4222

HSV2-UL30-1353 + GGAGGCGAGAUCGCUGAGGU 20 4223

HSV2-UL30-1354 + GAGAUCGCUGAGGUGGGACU 20 4224

HSV2-UL30-1355 + AGAUCGCUGAGGUGGGACUC 20 4225

HSV2-UL30-1356 + GAUCGCUGAGGUGGGACUCG 20 4226

HSV2-UL30-1357 + CGCUGAGGUGGGACUCGGGG 20 4227

HSV2-UL30-1358 + GUGGGACUCGGGGAGGUCGC 20 4228

HSV2-UL30-1359 + CGCAGGAUCCGAGCGAAAAC 20 4229

HSV2-UL30-1360 + AGGAUCCGAGCGAAAACAGG 20 4230

HSV2-UL30-1361 + AAAACAGGAGGAUGUGCUCG 20 4231

HSV2-UL30-1362 + AAACAGGAGGAUGUGCUCGA 20 4232

HSV2-UL30-1363 + CAGGAGGAUGUGCUCGAGGG 20 4233

HSV2-UL30-1364 + GAGGAUGUGCUCGAGGGCGG 20 4234

HSV2-UL30-1365 + GAUGUGCUCGAGGGCGGUGG 20 4235

HSV2-UL30-1366 + GCUCGAGGGCGGUGGUGGAC 20 4236

HSV2-UL30-1367 + GGACAGGUCGUAGAGCAGAC 20 4237

HSV2-UL30-1368 + CGUAGAGCAGACAGGAGAUC 20 4238

HSV2-UL30-1369 + GACAGGAGAUCUGGAUGACG 20 4239

HSV2-UL30-1370 + AUCUGGAUGACGAGGUCUUC 20 4240

HSV2-UL30-1371 + UCUGGAUGACGAGGUCUUCC 20 4241

HSV2-UL30-1372 + UCUUCCGGGCGUUCCGCGAC 20 4242

HSV2-UL30-1373 + CGGGCGUUCCGCGACCGGAA 20 4243

HSV2-UL30-1374 + GGCCAGCUCGUCCUCCCCCC 20 4244

HSV2-UL30-1375 + AUCGAAGCACAUGAGCUUGU 20 4245

HSV2-UL30-1376 + AAGCACAUGAGCUUGUAGGC 20 4246

HSV2-UL30-1377 + ACAUGAGCUUGUAGGCCGGC 20 4247

HSV2-UL30-1378 + GUAGGCCGGCAGGU CACACA 20 4248

HSV2-UL30-1379 + G U CACACAU GG CCCCCU CG A 20 4249

HSV2-UL30-1380 + ACAUGGCCCCCUCGACGGCC 20 4250

HSV2-UL30-1381 + GUUAAACUCGACGUCGCUCG 20 4251

HSV2-UL30-1382 + CUCGAGGUUCCGAACGCCGU 20 4252

HSV2-UL30-1383 + UCGAGGUUCCGAACGCCGUC 20 4253

HSV2-UL30-1384 + CGAGGUUCCGAACGCCGUCG 20 4254

HSV2-UL30-1385 + GAGGUUCCGAACGCCGUCGG 20 4255

HSV2-UL30-1386 + AGGUUCCGAACGCCGUCGGG 20 4256

HSV2-UL30-1387 + CCGAACGCCGUCGGGGGGCG 20 4257

HSV2-UL30-1388 + ACGCCGUCGGGGGGCGCGGU 20 4258

HSV2-UL30-1389 + CGCCGUCGGGGGGCGCGGUU 20 4259

HSV2-UL30-1390 + GUCGGGGGGCGCGGUUGGGC 20 4260

HSV2-UL30-1391 + GUUGGGCCGGCGCGUUCCCG 20 4261

HSV2-UL30-1392 + GGCCGGCGCGUUCCCGCGGC 20 4262

HSV2-UL30-1393 + GCCGGCGCGUUCCCGCGGCC 20 4263

HSV2-UL30-1394 + CGU UCCCGCGGCCGGGCUUG 20 4264

HSV2-UL30-1395 + UCCCGCGGCCGGGCUUGAGG 20 4265

HSV2-UL30-1396 + CU UGAGGCGGUACCAGCCGA 20 4266

HSV2-UL30-1397 + CAGCCGAAGGUGACAAACCC 20 4267 HSV2-UL30-1398 + AG CCG AAGG U G ACAAACCCC 20 4268

HSV2-UL30-1399 + U G ACAAACCCCG GGUUGUCC 20 4269

HSV2-UL30-1400 + CCGGGUUGUCCAGGAUAAAC 20 4270

HSV2-UL30-1401 + CGGGUUGUCCAGGAUAAACC 20 4271

HSV2-UL30-1402 + GUUGUCCAGGAUAAACCGGG 20 4272

HSV2-UL30-1403 + GUCCAGGAUAAACCGGGUGG 20 4273

HSV2-UL30-1404 + CCCCUCGUACUUCCUGAUCG 20 4274

HSV2-UL30-1405 + CCCUCGUACUUCCUGAUCGC 20 4275

HSV2-UL30-1406 + CCUCGUACUUCCUGAUCGCG 20 4276

HSV2-UL30-1407 + CG G G G CAAAAG UUGUCGCAC 20 4277

HSV2-UL30-1408 + GCAAAAGUUGUCGCACAGGU 20 4278

HSV2-UL30-1409 + GCCCGCUUCGCACGAAGACG 20 4279

HSV2-UL30-1410 + GCACGAAGACGCGGUAGUAC 20 4280

HSV2-UL30-1411 + CACGAAGACGCGGUAGUACA 20 4281

HSV2-UL30-1412 + AAGACGCGGUAGUACAGGGU 20 4282

HSV2-UL30-1413 + AGACGCGGUAGUACAGGGUC 20 4283

HSV2-UL30-1414 + CGU UUCGUAAUAGUACACGU 20 4284

HSV2-UL30-1415 + CCACCACCUCCGCCUCGAAG 20 4285

HSV2-UL30-1416 + CUCCGCCUCGAAGUGGUCCG 20 4286

HSV2-UL30-1417 + AGUGGUCCGCGGAGAUGCCG 20 4287

HSV2-UL30-1418 + AUGCCGCGGAACGACGCCCC 20 4288

HSV2-UL30-1419 + ACGCCCCCGGCGACUCGCGC 20 4289

HSV2-UL30-1420 + CGCCCCCGGCGACUCGCGCA 20 4290

HSV2-UL30-1421 + ACUCGCGCAGGGCCGCCGCC 20 4291

HSV2-UL30-1422 + AGGCGCUCGCAGAGAUCGCG 20 4292

HSV2-UL30-1423 + GGCGCUCGCAGAGAUCGCGC 20 4293

HSV2-UL30-1424 + GCGCUCGCAGAGAUCGCGCG 20 4294

HSV2-UL30-1425 + CGCAGAGAUCGCGCGGGGCA 20 4295

HSV2-UL30-1426 + CGCGCGGGGCACGGCACUGC 20 4296

HSV2-UL30-1427 + CCGCGUGCCGUAGACGUGAA 20 4297

HSV2-UL30-1428 + AGACGUGAACGGCGACGCGA 20 4298

HSV2-UL30-1429 + AACGGCGACGCGAUGGCCUU 20 4299

HSV2-UL30-1430 + ACGGCGACGCGAUGGCCUUC 20 4300

HSV2-UL30-1431 + CGGCGACGCGAUGGCCUUCG 20 4301

HSV2-UL30-1432 + GGCGACGCGAUGGCCUUCGG 20 4302

HSV2-UL30-1433 + CAGACCCAGAAGCGUGAUGA 20 4303

HSV2-UL30-1434 + CAGAAGCGUGAUGACGGUCC 20 4304

HSV2-UL30-1435 + AAGCGUGAUGACGGUCCCGG 20 4305

HSV2-UL30-1436 + AGCGUGAUGACGGUCCCGGC 20 4306

HSV2-UL30-1437 + GACGGUCCCGGCGGGCGUGA 20 4307

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-1439 + UUCCUCCGCCGUAGCGC 17 4309

HSV2-UL30-1440 + GUAGCGCCGGCCCCCGC 17 4310

HSV2-UL30-1441 + CGCCGGCCCGAACCCCG 17 4311

HSV2-UL30-1442 + CGCGGCCCUGAGCCGCG 17 4312

HSV2-UL30-1443 + CGCGCGGCCACGUCGUC 17 4313 HSV2-UL30-1444 + GCGCGGCCACGUCGUCC 17 4314

HSV2-UL30-1445 + CGCGGCCACGUCGUCCG 17 4315

HSV2-UL30-1446 + GCGGCCACGUCGUCCGG 17 4316

HSV2-UL30-1447 + CGGCCACGUCGUCCGGG 17 4317

HSV2-UL30-1448 + CGGGGGGUGCCACGUCU 17 4318

HSV2-UL30-1449 + GGGGGGUGCCACGUCUC 17 4319

HSV2-UL30-1450 + CCUCU UUAACAGACUCU 17 4320

HSV2-UL30-1451 + CAGACUCUCGGUGAUCU 17 4321

HSV2-UL30-1452 + UGGCGUUAUUUCCAAAC 17 4322

HSV2-UL30-1453 + GGCGUUAUUUCCAAACA 17 4323

HSV2-UL30-1454 + GGCCUUGAACGUCACGC 17 4324

HSV2-UL30-1455 + CGCAGGCCGCCCCCAGC 17 4325

HSV2-UL30-1456 + UAAUAGUCCGUGUUGAG 17 4326

HSV2-UL30-1457 + UGUUGAGCGGAACGCCC 17 4327

HSV2-UL30-1458 + GUUGAGCGGAACGCCCC 17 4328

HSV2-UL30-1459 + CGGAACGCCCCGGGCGA 17 4329

HSV2-UL30-1460 + CCGGGCGAUGGCGUACC 17 4330

HSV2-UL30-1461 + CGGGCGAUGGCGUACCC 17 4331

HSV2-UL30-1462 + GGGAUCCUCCGCCAGCU 17 4332

HSV2-UL30-1463 + CGGACACCAGCAGCUUG 17 4333

HSV2-UL30-1464 + GGACACCAGCAGCUUGC 17 4334

HSV2-UL30-1465 + GACACCAGCAGCUUGCG 17 4335

HSV2-UL30-1466 + CAGCAGCUUGCGGGGCU 17 4336

HSV2-UL30-1467 + GGCUUGGACGCGCCUCC 17 4337

HSV2-UL30-1468 + GCUUGGACGCGCCUCCC 17 4338

HSV2-UL30-1469 + CUUGGACGCGCCUCCCG 17 4339

HSV2-UL30-1470 + UUGGACGCGCCUCCCGG 17 4340

HSV2-UL30-1471 + UGGACGCGCCUCCCGGG 17 4341

HSV2-UL30-1472 + CGCGCCUCCCGGGGGGU 17 4342

HSV2-UL30-1473 + GGGGGGUCGGCAUGCGA 17 4343

HSV2-UL30-1474 + CAUGCGACGGCGUCUCC 17 4344

HSV2-UL30-1475 + AUGCGACGGCGUCUCCC 17 4345

HSV2-UL30-1476 + UGCGACGGCGUCUCCCG 17 4346

HSV2-UL30-1477 + GCGACGGCGUCUCCCGG 17 4347

HSV2-UL30-1478 + CGUCUCCCGGGGGCGCU 17 4348

HSV2-UL30-1479 + UCCCGGGGGCGCUUGGC 17 4349

HSV2-UL30-1480 + CCCGGGGGCGCUUGGCC 17 4350

HSV2-UL30-1481 + CCGGGGGCGCUUGGCCG 17 4351

HSV2-UL30-1482 + GGGGCGCUUGGCCGGGG 17 4352

HSV2-UL30-1483 + GGGCGCUUGGCCGGGGA 17 4353

HSV2-UL30-1484 + GCUUGGCCGGGGAGGGC 17 4354

HSV2-UL30-1485 + CUUGGCCGGGGAGGGCA 17 4355

HSV2-UL30-1486 + GGGGAGGGCAGGGCCGC 17 4356

HSV2-UL30-1487 + GGGAGGGCAGGGCCGCU 17 4357

HSV2-UL30-1488 + GGAGGGCAGGGCCGCUG 17 4358

HSV2-UL30-1489 + GAGGGCAGGGCCGCUGG 17 4359 HSV2-UL30-1490 + AGGGCAGGGCCGCUGGG 17 4360

HSV2-UL30-1491 + GGGCAGGGCCGCUGGGG 17 4361

HSV2-UL30-1492 + CAGGGCCGCUGGGGGGG 17 4362

HSV2-UL30-1493 + AGGGCCGCUGGGGGGGC 17 4363

HSV2-UL30-1494 + GGGGCGGGCUCGUCCCC 17 4364

HSV2-UL30-1495 + GGGCGGGCUCGUCCCCU 17 4365

HSV2-UL30-1496 + GGCGGGCUCGUCCCCUG 17 4366

HSV2-UL30-1497 + GGGCUCGUCCCCUGGGG 17 4367

HSV2-UL30-1498 + CUCGUCCCCUGGGGCGG 17 4368

HSV2-UL30-1499 + CGGCGGCGUCUAGCUCG 17 4369

HSV2-UL30-1500 + CGGCGUCUAGCUCGCGG 17 4370

HSV2-UL30-1501 + GGCGUCUAGCUCGCGGA 17 4371

HSV2-UL30-1502 + GUCUAGCUCGCGGAGGG 17 4372

HSV2-UL30-1503 + CCGUCUCCUCUACCUCG 17 4373

HSV2-UL30-1504 + CGUCUCCUCUACCUCGC 17 4374

HSV2-UL30-1505 + CCUCUACCUCGCGGGUC 17 4375

HSV2-UL30-1506 + CUCUACCUCGCGGGUCU 17 4376

HSV2-UL30-1507 + UGGGCCACGAUCACGUA 17 4377

HSV2-UL30-1508 + GGGCCACGAUCACGUAC 17 4378

HSV2-UL30-1509 + CGAUCACGUACGGGAUC 17 4379

HSV2-UL30-1510 + CGGGAUCCGGUCCUUGA 17 4380

HSV2-UL30-1511 + AUCCGGUCCUUGAUGGA 17 4381

HSV2-UL30-1512 + UCCGGUCCUUGAUGGAC 17 4382

HSV2-UL30-1513 + UGGACGGGACCUGCGCG 17 4383

HSV2-UL30-1514 + ACGGGACCUGCGCGCGG 17 4384

HSV2-UL30-1515 + CGGGACCUGCGCGCGGC 17 4385

HSV2-UL30-1516 + GCUUGUAAUACACCGUC 17 4386

HSV2-UL30-1517 + UGUAAUACACCGUCAGG 17 4387

HSV2-UL30-1518 + GUAAUACACCGUCAGGU 17 4388

HSV2-UL30-1519 + ACACCGUCAGGUGGGCC 17 4389

HSV2-UL30-1520 + GUGGGCCAGGCGCUUGU 17 4390

HSV2-UL30-1521 + UUGUUGGUGUACGCGCG 17 4391

HSV2-UL30-1522 + UGUUGGUGUACGCGCGC 17 4392

HSV2-UL30-1523 + CGGGUGUCUGCUCAGUU 17 4393

HSV2-UL30-1524 + GUGUCUGCUCAGUUCGG 17 4394

HSV2-UL30-1525 + UGCUCAGUUCGGCGGUG 17 4395

HSV2-UL30-1526 + CGGUGAGGACAAAGUCC 17 4396

HSV2-UL30-1527 + UCCUGGAUGUCCCUCUC 17 4397

HSV2-UL30-1528 + CCUGGAUGUCCCUCUCC 17 4398

HSV2-UL30-1529 + GAUGUCCCUCUCCGGGU 17 4399

HSV2-UL30-1530 + GGUCGGUGAUGCGCCGA 17 4400

HSV2-UL30-1531 + GUCGGUGAUGCGCCGAU 17 4401

HSV2-UL30-1532 + GCCGAUGGGCGUCUACG 17 4402

HSV2-UL30-1533 + AUGGGCGUCUACGAGGA 17 4403

HSV2-UL30-1534 + GAACGCCUGCAGUCCCU 17 4404

HSV2-UL30-1535 + AACGCCUGCAGUCCCUC 17 4405 HSV2-UL30-1536 + CCUGCAGUCCCUCGGGC 17 4406

HSV2-UL30-1537 + CUGCAGUCCCUCGGGCA 17 4407

HSV2-UL30-1538 + UGCAGUCCCUCGGGCAG 17 4408

HSV2-UL30-1539 + CGCCAGCCACUCCUCUG 17 4409

HSV2-UL30-1540 + GCCAGCCACUCCUCUGC 17 4410

HSV2-UL30-1541 + CCAGCCACUCCUCUGCG 17 4411

HSV2-UL30-1542 + CUCCUCUGCGGGGCGCU 17 4412

HSV2-UL30-1543 + GGGGCGCUCGGCUAACG 17 4413

HSV2-UL30-1544 + GCGCUCGGCUAACGCGG 17 4414

HSV2-UL30-1545 + UAACGCGGCGGCCGCUC 17 4415

HSV2-UL30-1546 + GGCGGCCGCUCCGGAUA 17 4416

HSV2-UL30-1547 + UAUCGUCGUAAAACAGC 17 4417

HSV2-UL30-1548 + AAAACAG CAGGUCGACC 17 4418

HSV2-UL30-1549 + AAACAG CAGGUCGACCA 17 4419

HSV2-UL30-1550 + CAGGUCGACCAGGGCCC 17 4420

HSV2-UL30-1551 + GUCGACCAGGGCCCUGG 17 4421

HSV2-UL30-1552 + CCAGGGCCCUGGAGGUG 17 4422

HSV2-UL30-1553 + GCCGAUGUACUUUUUCU 17 4423

HSV2-UL30-1554 + GGCGAUGAGCAGCAGCU 17 4424

HSV2-UL30-1555 + UCGCACUCGAGCUUGAU 17 4425

HSV2-UL30-1556 + CGCACUCGAGCUUGAUC 17 4426

HSV2-UL30-1557 + GCACUCGAGCUUGAUCG 17 4427

HSV2-UL30-1558 + CACUCGAGCUUGAUCGG 17 4428

HSV2-UL30-1559 + ACUCGAGCUUGAUCGGG 17 4429

HSV2-UL30-1560 + CGAGCUUGAUCGGGGGG 17 4430

HSV2-UL30-1561 + ACAGCGCGCGCGAGAUG 17 4431

HSV2-UL30-1562 + CGCCAUCUUGUCGCCCA 17 4432

HSV2-UL30-1563 + UGUCGCCCAUGGCCACC 17 4433

HSV2-UL30-1564 + CAUGGCCACCAGGCCCG 17 4434

HSV2-UL30-1565 + CCAGGCCCGCGGCCGUG 17 4435

HSV2-UL30-1566 + CCGCGGCCGUGAGGCCG 17 4436

HSV2-UL30-1567 + GCGGCACAAAACGAAAA 17 4437

HSV2-UL30-1568 + CCCGUAGAUGAUGCGCA 17 4438

HSV2-UL30-1569 + AUGAUGCGCAUGGAGUA 17 4439

HSV2-UL30-1570 + GCGCAUGGAGUACGGAC 17 4440

HSV2-UL30-1571 + CGCAUGGAGUACGGACC 17 4441

HSV2-UL30-1572 + GCAUGGAGUACGGACCG 17 4442

HSV2-UL30-1573 + CAUGGAGUACGGACCGG 17 4443

HSV2-UL30-1574 + ACCGGGGGCGCGCAUGC 17 4444

HSV2-UL30-1575 + CGCAUGCCGGCCGCCUC 17 4445

HSV2-UL30-1576 + GGCCGCCUCCGGAAAGU 17 4446

HSV2-UL30-1577 + CGUACGCGCGCGUCGCG 17 4447

HSV2-UL30-1578 + UCGCGAGGAGCAUCUCG 17 4448

HSV2-UL30-1579 + GAGCAUCUCGCGGCCGA 17 4449

HSV2-UL30-1580 + GCGGCCGAUGGUCGUCA 17 4450

HSV2-UL30-1581 + GCCGAUGGUCGUCACGG 17 4451 HSV2-UL30-1582 + GAUGGUCGUCACGGUGG 17 4452

HSV2-UL30-1583 + CGGUGGCGGCCACGUGC 17 4453

HSV2-UL30-1584 + GGCGGCCACGUGCAGGC 17 4454

HSV2-UL30-1585 + GCGGCCACGUGCAGGCA 17 4455

HSV2-UL30-1586 + AAGACCGUGCUGCACCC 17 4456

HSV2-UL30-1587 + G U U G CACACCACCU U G A 17 4457

HSV2-UL30-1588 + GCACACCACCUUGAUGG 17 4458

HSV2-UL30-1589 + CGGCCUGUUGCUUGUCG 17 4459

HSV2-UL30-1590 + CCUGUUGCUUGUCGAGG 17 4460

HSV2-UL30-1591 + UUGCUUGUCGAGGAGGA 17 4461

HSV2-UL30-1592 + GAGGAGGACGGCCUCCU 17 4462

HSV2-UL30-1593 + AGGAGGACGGCCUCCUC 17 4463

HSV2-UL30-1594 + GGAGGACGGCCUCCUCG 17 4464

HSV2-UL30-1595 + GAGGACGGCCUCCUCGG 17 4465

HSV2-UL30-1596 + CCUCCUCGGGGGUGCUC 17 4466

HSV2-UL30-1597 + CUCCUCGGGGGUGCUCU 17 4467

HSV2-UL30-1598 + UCCUCGGGGGUGCUCUG 17 4468

HSV2-UL30-1599 + CCUCGGGGGUGCUCUGG 17 4469

HSV2-UL30-1600 + UCUGGGGGAUCCGCGAG 17 4470

HSV2-UL30-1601 + GCGGAUCUGCUUUCGCA 17 4471

HSV2-UL30-1602 + CCAGCCAGUCGCGCAGC 17 4472

HSV2-UL30-1603 + GCAGCAGGAUGCUCAGC 17 4473

HSV2-UL30-1604 + GCAGGCUCUCGCGUACG 17 4474

HSV2-UL30-1605 + CAGGCUCUCGCGUACGU 17 4475

HSV2-UL30-1606 + U CACG AAG AACAGCCG U 17 4476

HSV2-UL30-1607 + CCCCCACCUCGAUCUCC 17 4477

HSV2-UL30-1608 + CGAUCUCCAGGUAGUCC 17 4478

HSV2-UL30-1609 + AGUCCCGGUCCGCCUCC 17 4479

HSV2-UL30-1610 + CGCCUCCAGGUGCGCGA 17 4480

HSV2-UL30-1611 + CAGGUGCGCGACGGCCU 17 4481

HSV2-UL30-1612 + AGGUGCGCGACGGCCUC 17 4482

HSV2-UL30-1613 + CGACGGCCUCGGGCCGC 17 4483

HSV2-UL30-1614 + GACGGCCUCGGGCCGCA 17 4484

HSV2-UL30-1615 + AGAGCGUACUGAAGCAC 17 4485

HSV2-UL30-1616 + UACUGAAGCACAGGUUG 17 4486

HSV2-UL30-1617 + ACUGAAGCACAGGUUGU 17 4487

HSV2-UL30-1618 + AGCACAGGUUGUGGGCC 17 4488

HSV2-UL30-1619 + GUGGGCCUGGAUGAUGC 17 4489

HSV2-UL30-1620 + UGGGCCUGGAUGAUGCU 17 4490

HSV2-UL30-1621 + GGGCCUGGAUGAUGCUG 17 4491

HSV2-UL30-1622 + GGAUGAUGCUGGGGUAC 17 4492

HSV2-UL30-1623 + GAUGCUGGGGUACAGGC 17 4493

HSV2-UL30-1624 + AAAG U CAAACACCACCA 17 4494

HSV2-UL30-1625 + AAG U CAAACACCACCAC 17 4495

HSV2-UL30-1626 + AG U CAAACACCACCACG 17 4496

HSV2-UL30-1627 + GGGGUCGACGUGAAACC 17 4497 HSV2-UL30-1628 + G U CGACG U G AAACCCG G 17 4498

HSV2-UL30-1629 + GACGUGAAACCCGGAGG 17 4499

HSV2-UL30-1630 + ACG U G AAACCCG GAGG U 17 4500

HSV2-UL30-1631 + CGUGAAACCCGGAGGUG 17 4501

HSV2-UL30-1632 + ACCCGGAGGUGGGGUCG 17 4502

HSV2-UL30-1633 + AGGUGGGGUCGAGGACC 17 4503

HSV2-UL30-1634 + GGUGGGGUCGAGGACCC 17 4504

HSV2-UL30-1635 + CGAGGACCCGGGCCCCC 17 4505

HSV2-UL30-1636 + CCUGGUACCCAACGUGC 17 4506

HSV2-UL30-1637 + CCCAACGUGCCGGCCCC 17 4507

HSV2-UL30-1638 + UCCCCGUUCCCGUCCCC 17 4508

HSV2-UL30-1639 + CGCUCCCCUUCCCCCCG 17 4509

HSV2-UL30-1640 + CCCU U CCCCCCG AG GCA 17 4510

HSV2-UL30-1641 + UCCCCCCGAGGCACGGC 17 4511

HSV2-UL30-1642 + CCCCCCGAGGCACGGCC 17 4512

HSV2-UL30-1643 + AGGCACGGCCGGGCGCU 17 4513

HSV2-UL30-1644 + GGCACGGCCGGGCGCU U 17 4514

HSV2-UL30-1645 + UGGGCGCCUCCUUGUCG 17 4515

HSV2-UL30-1646 + GGCCCCGAAACCGCCCC 17 4516

HSV2-UL30-1647 + GCCCCGAAACCGCCCCU 17 4517

HSV2-UL30-1648 + AACCGCCCCUGGGUGUC 17 4518

HSV2-UL30-1649 + GCCCCUGGGUGUCCGGC 17 4519

HSV2-UL30-1650 + GCAGGAUGAAGCCCUUC 17 4520

HSV2-UL30-1651 + AGCCCUUCUGGCCCGCA 17 4521

HSV2-UL30-1652 + UCUGGCCCGCAAGGCGC 17 4522

HSV2-UL30-1653 + GGCCCGCAAGGCGCAGG 17 4523

HSV2-UL30-1654 + GGAGGCACGUGAAGACG 17 4524

HSV2-UL30-1655 + UGAAGACGCGGAUCUGC 17 4525

HSV2-UL30-1656 + CUGCUGGCCGUCGUAGA 17 4526

HSV2-UL30-1657 + GGCCGUCGUAGAUGGUG 17 4527

HSV2-UL30-1658 + GCCGUCGUAGAUGGUGC 17 4528

HSV2-UL30-1659 + UGAUGUUGAUGCCCGCC 17 4529

HSV2-UL30-1660 + GCCCGCCAGGCGCGCGA 17 4530

HSV2-UL30-1661 + CGCCAGGCGCGCGACGG 17 4531

HSV2-UL30-1662 + CGACGGCGGAAAGCUCC 17 4532

HSV2-UL30-1663 + GCGGAAAGCUCCAGGUG 17 4533

HSV2-UL30-1664 + GAUCACCCCGCGCUGCG 17 4534

HSV2-UL30-1665 + AUCACCCCGCGCUGCGC 17 4535

HSV2-UL30-1666 + CCCGCGCUGCGCGGGCC 17 4536

HSV2-UL30-1667 + GCGCUGCGCGGGCCCGG 17 4537

HSV2-UL30-1668 + GGGCCCGGAGGCGUAGU 17 4538

HSV2-UL30-1669 + CCCGGAGGCGUAGUAGG 17 4539

HSV2-UL30-1670 + CCGGAGGCGUAGUAGGC 17 4540

HSV2-UL30-1671 + CGGAGGCGUAGUAGGCG 17 4541

HSV2-UL30-1672 + AGUAGGCGGGGAUGUCG 17 4542

HSV2-UL30-1673 + CUUCUUGUCCUUCAAGA 17 4543 HSV2-UL30-1674 + GUCCUUCAAGACGGCCU 17 4544

HSV2-UL30-1675 + CAAGACGGCCUCGGCGA 17 4545

HSV2-UL30-1676 + GGCGUUCAGCUUGUAGC 17 4546

HSV2-UL30-1677 + GGAGAGUUUGACCUUGU 17 4547

HSV2-UL30-1678 + UGCUGCGCUUCUGAAAG 17 4548

HSV2-UL30-1679 + GCUUCUGAAAGUGGCUC 17 4549

HSV2-UL30-1680 + GGCCGAUGUCCCACACG 17 4550

HSV2-UL30-1681 + ACACGCGGAACACACCC 17 4551

HSV2-UL30-1682 + CGCCCGUACCCGUCGAG 17 4552

HSV2-UL30-1683 + GCCCGUACCCGUCGAGC 17 4553

HSV2-UL30-1684 + GUAGAUCUCCGUCAGCU 17 4554

HSV2-UL30-1685 + UCUCCGUCAGCUUGGUC 17 4555

HSV2-UL30-1686 + CAGCUUGGUCAGGACGA 17 4556

HSV2-UL30-1687 + AGCUUGGUCAGGACGAA 17 4557

HSV2-UL30-1688 + GUUGAUGAUGUUGUACC 17 4558

HSV2-UL30-1689 + GUACCCGGUCACGAACU 17 4559

HSV2-UL30-1690 + UACCCGGUCACGAACUC 17 4560

HSV2-UL30-1691 + ACCCGGUCACGAACUCG 17 4561

HSV2-UL30-1692 + GCCGUACUGCUUGACGA 17 4562

HSV2-UL30-1693 + CUUGACGAAGGUCAUGA 17 4563

HSV2-UL30-1694 + AUUCGCUGU CAAACU CC 17 4564

HSV2-UL30-1695 + GUCAAACUCCAGGACGA 17 4565

HSV2-UL30-1696 + UCAAACUCCAGGACGAC 17 4566

HSV2-UL30-1697 + CAAACUCCAGGACGACG 17 4567

HSV2-UL30-1698 + AAACUCCAGGACGACGG 17 4568

HSV2-UL30-1699 + UCCAGGACGACGGGGGC 17 4569

HSV2-UL30-1700 + GGACGACGGGGGCCGGC 17 4570

HSV2-UL30-1701 + GGGGGCCGGCAGGCCCC 17 4571

HSV2-UL30-1702 + GGCCGGCAGGCCCCUGG 17 4572

HSV2-UL30-1703 + UGGAGGCGAGAUCGCUG 17 4573

HSV2-UL30-1704 + AGGCGAGAUCGCUGAGG 17 4574

HSV2-UL30-1705 + GGCGAGAUCGCUGAGGU 17 4575

HSV2-UL30-1706 + AUCGCUGAGGUGGGACU 17 4576

HSV2-UL30-1707 + UCGCUGAGGUGGGACUC 17 4577

HSV2-UL30-1708 + CGCUGAGGUGGGACUCG 17 4578

HSV2-UL30-1709 + UGAGGUGGGACUCGGGG 17 4579

HSV2-UL30-1710 + GGACUCGGGGAGGUCGC 17 4580

HSV2-UL30-1711 + AGGAUCCGAGCGAAAAC 17 4581

HSV2-UL30-1712 + AUCCGAGCGAAAACAGG 17 4582

HSV2-UL30-1713 + ACAGGAGGAUGUGCUCG 17 4583

HSV2-UL30-1714 + CAGGAGGAUGUGCUCGA 17 4584

HSV2-UL30-1715 + GAGGAUGUGCUCGAGGG 17 4585

HSV2-UL30-1716 + GAUGUGCUCGAGGGCGG 17 4586

HSV2-UL30-1717 + GUGCUCGAGGGCGGUGG 17 4587

HSV2-UL30-1718 + CGAGGGCGGUGGUGGAC 17 4588

HSV2-UL30-1719 + CAGGUCGUAGAGCAGAC 17 4589 HSV2-UL30-1720 + AGAGCAGACAGGAGAUC 17 4590

HSV2-UL30-1721 + AGGAGAUCUGGAUGACG 17 4591

HSV2-UL30-1722 + UGGAUGACGAGGUCUUC 17 4592

HSV2-UL30-1723 + GGAUGACGAGGUCUUCC 17 4593

HSV2-UL30-1724 + UCCGGGCGUUCCGCGAC 17 4594

HSV2-UL30-1725 + GCGUUCCGCGACCGGAA 17 4595

HSV2-UL30-1726 + CAGCUCGUCCUCCCCCC 17 4596

HSV2-UL30-1727 + GAAGCACAUGAGCUUGU 17 4597

HSV2-UL30-1728 + CACAUGAGCUUGUAGGC 17 4598

HSV2-UL30-1729 + UGAGCUUGUAGGCCGGC 17 4599

HSV2-UL30-1730 + GGCCGGCAGGUCACACA 17 4600

HSV2-UL30-1731 + ACACAUGGCCCCCUCGA 17 4601

HSV2-UL30-1732 + UGGCCCCCUCGACGGCC 17 4602

HSV2-UL30-1733 + AAACUCGACGUCGCUCG 17 4603

HSV2-UL30-1734 + GAGGUUCCGAACGCCGU 17 4604

HSV2-UL30-1735 + AGGUUCCGAACGCCGUC 17 4605

HSV2-UL30-1736 + GGUUCCGAACGCCGUCG 17 4606

HSV2-UL30-1737 + GUUCCGAACGCCGUCGG 17 4607

HSV2-UL30-1738 + UUCCGAACGCCGUCGGG 17 4608

HSV2-UL30-1739 + AACGCCGUCGGGGGGCG 17 4609

HSV2-UL30-1740 + CCGUCGGGGGGCGCGGU 17 4610

HSV2-UL30-1741 + CGUCGGGGGGCGCGGUU 17 4611

HSV2-UL30-1742 + GGGGGGCGCGGUUGGGC 17 4612

HSV2-UL30-1743 + GGGCCGGCGCGUUCCCG 17 4613

HSV2-UL30-1744 + CGGCGCGU UCCCGCGGC 17 4614

HSV2-UL30-1745 + GGCGCGUUCCCGCGGCC 17 4615

HSV2-UL30-1746 + UCCCGCGGCCGGGCUUG 17 4616

HSV2-UL30-1747 + CGCGGCCGGGCUUGAGG 17 4617

HSV2-UL30-1748 + GAGGCGGUACCAGCCGA 17 4618

HSV2-UL30-1749 + CCG AAGG U G ACAAACCC 17 4619

HSV2-UL30-1750 + CGAAG G U G ACAAACCCC 17 4620

HSV2-UL30-1751 + CAAACCCCGGGUUGUCC 17 4621

HSV2-UL30-1752 + GGUUGUCCAGGAUAAAC 17 4622

HSV2-UL30-1753 + GUUGUCCAGGAUAAACC 17 4623

HSV2-UL30-1754 + GUCCAGGAUAAACCGGG 17 4624

HSV2-UL30-1755 + CAGGAUAAACCGGGUGG 17 4625

HSV2-UL30-1756 + CUCGUACUUCCUGAUCG 17 4626

HSV2-UL30-1757 + UCGUACUUCCUGAUCGC 17 4627

HSV2-UL30-1758 + CGUACUUCCUGAUCGCG 17 4628

HSV2-UL30-1759 + G G CAAAAG UUGUCGCAC 17 4629

HSV2-UL30-1760 + AAAG UUGUCGCACAGGU 17 4630

HSV2-UL30-1761 + CGCUUCGCACGAAGACG 17 4631

HSV2-UL30-1762 + CGAAG ACGCGGUAGU AC 17 4632

HSV2-UL30-1763 + GAAGACGCGGUAGUACA 17 4633

HSV2-UL30-1764 + ACGCGGUAGUACAGGGU 17 4634

HSV2-UL30-1765 + CGCGGUAGUACAGGGUC 17 4635 HSV2-UL30-1766 + UUCGUAAUAGUACACGU 17 4636

HSV2-UL30-1767 + CCACCUCCGCCUCGAAG 17 4637

HSV2-UL30-1768 + CGCCUCGAAGUGGUCCG 17 4638

HSV2-UL30-1769 + GGUCCGCGGAGAUGCCG 17 4639

HSV2-UL30-1770 + CCGCGGAACGACGCCCC 17 4640

HSV2-UL30-1771 + CCCCCGGCGACUCGCGC 17 4641

HSV2-UL30-1772 + CCCCGGCGACUCGCGCA 17 4642

HSV2-UL30-1773 + CGCGCAGGGCCGCCGCC 17 4643

HSV2-UL30-1774 + CGCUCGCAGAGAUCGCG 17 4644

HSV2-UL30-1775 + GCUCGCAGAGAUCGCGC 17 4645

HSV2-UL30-1776 + CUCGCAGAGAUCGCGCG 17 4646

HSV2-UL30-1777 + AGAGAUCGCGCGGGGCA 17 4647

HSV2-UL30-1778 + GCGGGGCACGGCACUGC 17 4648

HSV2-UL30-1779 + CGUGCCGUAGACGUGAA 17 4649

HSV2-UL30-1780 + CGUGAACGGCGACGCGA 17 4650

HSV2-UL30-1781 + GGCGACGCGAUGGCCUU 17 4651

HSV2-UL30-1782 + GCGACGCGAUGGCCUUC 17 4652

HSV2-UL30-1783 + CGACGCGAUGGCCUUCG 17 4653

HSV2-UL30-1784 + GACGCGAUGGCCUUCGG 17 4654

HSV2-UL30-1785 + ACCCAGAAGCGUGAUGA 17 4655

HSV2-UL30-1786 + AAGCGUGAUGACGGUCC 17 4656

HSV2-UL30-1787 + CGUGAUGACGGUCCCGG 17 4657

HSV2-UL30-1788 + GUGAUGACGGUCCCGGC 17 4658

HSV2-UL30-1789 + GGUCCCGGCGGGCGUGA 17 4659

HSV2-UL30-1790 + CGUCCAUAAAUCGCUCG 17 4660

Table 2D provides exemplary targeting domains for knocking out the UL30 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL30 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2D

1st Tier Target

DNA

g NA Name Targeting Domain Site

Strand

Length Seq ID

HSV2-UL30-247 - CGCGGGCGGCCCGGCUUCCC 20 4661

HSV2-UL30-2 - GCGGGCGGCCCGGCUUCCCC 20 3158

HSV2-UL30-3 - CGGGCGGCCCGGCUUCCCCC 20 3159

HSV2-UL30-4 - GGGCGGCCCGGCUUCCCCCG 20 3160

HSV2-UL30-5 - GGCGGCCCGGCUUCCCCCGG 20 3161

HSV2-UL30-6 - GCGGCCCGGCUUCCCCCGGG 20 3162

HSV2-UL30-253 - CCCGGGGGGAAGCCGGCGGC 20 4662

HSV2-UL30-254 - GCCGGCGGCUCGGGCGGCGU 20 4663

HSV2-UL30-255 - U U U U U U G CCCCCCACAACCC 20 4664

HSV2-UL30-14 - U U U U U G CCCCCCACAACCCC 20 3170

HSV2-UL30-15 - U U U U G CCCCCCACAACCCCC 20 3171

HSV2-UL30-16 - U U U G CCCCCCACAACCCCCG 20 3172

HSV2-UL30-259 - CACCGCCGCCUUGCCGCCGG 20 4665

HSV2-UL30-260 - CAACCCCCACCU CGCU CAG A 20 4666

HSV2-UL30-19 - AACCCCCACCU CGCU CAG AC 20 3174

HSV2-UL30-262 - CGGAACGCAGCCAAAGGCCC 20 4667

HSV2-UL30-263 - UCAGCGCCAUACGUACUACA 20 4668

HSV2-UL30-264 - UACGUACUACAGCGAGUGCG 20 4669

HSV2-UL30-265 - GAUUUAUCGCCCCGCGUUCG 20 4670

HSV2-UL30-266 - UAUCGCCCCGCGUUCGCUGG 20 4671

HSV2-UL30-267 - UCGCCCCGCGUUCGCUGGAC 20 4672

HSV2-UL30-268 - CGCUGGACGAGGACGCCCCC 20 4673

HSV2-UL30-26 - GCUGGACGAGGACGCCCCCG 20 3179

HSV2-UL30-270 - CGCCCCCGCGGAGCAGCGCA 20 4674

HSV2-UL30-27 - GCCCCCGCGGAGCAGCGCAC 20 3180

HSV2-UL30-272 - GCGCGCCCCUAAGGUGUACU 20 4675

HSV2-UL30-33 - CGCGCCCCUAAGGUGUACUG 20 3071

HSV2-UL30-34 - GCGCCCCUAAGGUGUACUGC 20 3072

HSV2-UL30-35 - CGCCCCUAAGGUGUACUGCG 20 3185

HSV2-UL30-36 - GCCCCUAAGGUGUACUGCGG 20 3073

HSV2-UL30-37 - CCCCUAAGGUGUACUGCGGG 20 3074

HSV2-UL30-278 - UAAGGUGUACUGCGGGGGGG 20 4676

HSV2-UL30-279 - ACGAGCGCGACGUCCUCCGC 20 4677

HSV2-UL30-280 - GCGACGUCCUCCGCGUGGGC 20 4678

HSV2-UL30-41 - CGACGUCCUCCGCGUGGGCC 20 3186

HSV2-UL30-282 - ACGUCCUCCGCGUGGGCCCG 20 4679

HSV2-UL30-283 - UGGCCGCGUCGCUUGCGCCU 20 4680

HSV2-UL30-45 - GGCCGCGUCGCUUGCGCCUG 20 3189

HSV2-UL30-285 - GCU UGCGCCUGUGGGGCGGU 20 4681

HSV2-UL30-286 - GGGCGGUGCGGACCAUGCCC 20 4682

HSV2-UL30-287 - GCGGUGCGGACCAUGCCCCC 20 4683

HSV2-UL30-50 - CGGUGCGGACCAUGCCCCCG 20 3082

HSV2-UL30-289 - UCUUCCACGUGUACGACAUC 20 4684 HSV2-UL30-53 - CU U CCACG U G U ACG ACAU CC 20 3084

HSV2-UL30-291 - UGUACGACAUCCUGGAGCAC 20 4685

HSV2-UL30-54 - GUACGACAUCCUGGAGCACG 20 3192

HSV2-UL30-293 - GGGCGGCCCGGCU UCCC 17 4686

HSV2-UL30-125 - GGCGGCCCGGCUUCCCC 17 3236

HSV2-UL30-126 - GCGGCCCGGCUUCCCCC 17 3237

HSV2-UL30-127 - CGGCCCGGCUUCCCCCG 17 3238

HSV2-UL30-128 - GGCCCGGCUUCCCCCGG 17 3239

HSV2-UL30-129 - GCCCGGCUUCCCCCGGG 17 3240

HSV2-UL30-299 - GGGGGGAAGCCGGCGGC 17 4687

HSV2-UL30-300 - GGCGGCUCGGGCGGCGU 17 4688

HSV2-UL30-301 - U U U GCCCCCCACAACCC 17 4689

HSV2-UL30-137 - UUGCCCCCCACAACCCC 17 3248

HSV2-UL30-138 - UGCCCCCCACAACCCCC 17 3249

HSV2-UL30-139 - GCCCCCCACAACCCCCG 17 3250

HSV2-UL30-305 - CGCCGCCUUGCCGCCGG 17 4690

HSV2-UL30-306 - CCCCCACCUCGCUCAGA 17 4691

HSV2-UL30-142 - CCCCACCUCGCUCAGAC 17 3252

HSV2-UL30-308 - AACGCAGCCAAAGGCCC 17 4692

HSV2-UL30-309 - GCGCCAUACGUACUACA 17 4693

HSV2-UL30-310 - GUACUACAGCGAGUGCG 17 4694

HSV2-UL30-311 - UUAUCGCCCCGCGUUCG 17 4695

HSV2-UL30-312 - CGCCCCGCGUUCGCUGG 17 4696

HSV2-UL30-313 - CCCCGCGUUCGCUGGAC 17 4697

HSV2-UL30-314 - UGGACGAGGACGCCCCC 17 4698

HSV2-UL30-149 - GGACGAGGACGCCCCCG 17 3257

HSV2-UL30-316 - CCCCGCGGAGCAGCGCA 17 4699

HSV2-UL30-150 - CCCGCGGAGCAGCGCAC 17 3258

HSV2-UL30-318 - CGCCCCUAAGGUGUACU 17 4700

HSV2-UL30-156 - GCCCCUAAGGUGUACUG 17 3116

HSV2-UL30-157 - CCCCUAAGGUGUACUGC 17 3117

HSV2-UL30-158 - CCCUAAGGUGUACUGCG 17 3263

HSV2-UL30-159 - CCUAAGGUGUACUGCGG 17 3118

HSV2-UL30-160 - CUAAGGUGUACUGCGGG 17 3119

HSV2-UL30-324 - GGUGUACUGCGGGGGGG 17 4701

HSV2-UL30-325 - AGCGCGACGUCCUCCGC 17 4702

HSV2-UL30-326 - ACGUCCUCCGCGUGGGC 17 4703

HSV2-UL30-164 - CGUCCUCCGCGUGGGCC 17 3264

HSV2-UL30-328 - UCCUCCGCGUGGGCCCG 17 4704

HSV2-UL30-329 - CCGCGUCGCUUGCGCCU 17 4705

HSV2-UL30-168 - CGCGUCGCUUGCGCCUG 17 3267

HSV2-UL30-331 - UGCGCCUGUGGGGCGGU 17 4706

HSV2-UL30-332 - CGGUGCGGACCAUGCCC 17 4707

HSV2-UL30-333 - GUGCGGACCAUGCCCCC 17 4708

HSV2-UL30-173 - UGCGGACCAUGCCCCCG 17 3127

HSV2-UL30-335 - UCCACGUGUACGACAUC 17 4709 HSV2-UL30-176 - CCACGUGUACGACAUCC 17 3129

HSV2-UL30-337 - ACGACAUCCUGGAGCAC 17 4710

HSV2-UL30-177 - CGACAUCCUGGAGCACG 17 3270

HSV2-UL30-339 + UACGCGUGUUCCACGUGCUC 20 4711

HSV2-UL30-340 + UGCUCCAGGAUGUCGUACAC 20 4712

HSV2-UL30-56 + GCUCCAGGAUGUCGUACACG 20 3194

HSV2-UL30-342 + ACACGUGGAAGACGGUGACG 20 4713

HSV2-UL30-59 + CACGUGGAAGACGGUGACGG 20 3197

HSV2-UL30-344 + GGAAGACGGUGACGGUGGGG 20 4714

HSV2-UL30-345 + UGACGGUGGGGUCGAACCCC 20 4715

HSV2-UL30-62 + GACGGUGGGGUCGAACCCCU 20 3086

HSV2-UL30-63 + ACGGUGGGGUCGAACCCCUC 20 3087

HSV2-UL30-348 + ACAGGCGCAAGCGACGCGGC 20 4716

HSV2-UL30-349 + GCGACGCGGCCAGAAGCCCU 20 4717

HSV2-UL30-350 + CAGAAGCCCUCCGGGCCCAC 20 4718

HSV2-UL30-71 + AGAAGCCCUCCGGGCCCACG 20 3201

HSV2-UL30-352 + AAGCCCUCCGGGCCCACGCG 20 4719

HSV2-UL30-353 + GUCCCCCCCGCAGUACACCU 20 4720

HSV2-UL30-73 + UCCCCCCCGCAGUACACCUU 20 3203

HSV2-UL30-355 + CAGUACACCUUAGGGGCGCG 20 4721

HSV2-UL30-76 + AGUACACCUUAGGGGCGCGC 20 3095

HSV2-UL30-357 + GCGCGCCGGAGGCGGCCGUC 20 4722

HSV2-UL30-358 + GGACCCCGGUGCGCUGCUCC 20 4723

HSV2-UL30-81 + GACCCCGGUGCGCUGCUCCG 20 3207

HSV2-UL30-82 + ACCCCGGUGCGCUGCUCCGC 20 3208

HSV2-UL30-361 + CGCGGGGGCGUCCUCGUCCA 20 4724

HSV2-UL30-362 + GGCGUCCUCGUCCAGCGAAC 20 4725

HSV2-UL30-85 + GCGUCCUCGUCCAGCGAACG 20 3098

HSV2-UL30-364 + AGCGAACGCGGGGCGAUAAA 20 4726

HSV2-UL30-365 + UCGCUGUAGUACGUAUGGCG 20 4727

HSV2-UL30-366 + ACGUAUGGCGCUGAGCCGGC 20 4728

HSV2-UL30-367 + GUAUGGCGCUGAGCCGGCCC 20 4729

HSV2-UL30-368 + GCCUUUGGCUGCGUUCCGGU 20 4730

HSV2-UL30-369 + UUGGCUGCGUUCCGGUCUGA 20 4731

HSV2-UL30-370 + UGCGUUCCGGUCUGAGCGAG 20 4732

HSV2-UL30-95 + GCGUUCCGGUCUGAGCGAGG 20 3104

HSV2-UL30-96 + CGUUCCGGUCUGAGCGAGGU 20 3214

HSV2-UL30-373 + UCUGAGCGAGGUGGGGGUUG 20 4733

HSV2-UL30-374 + CGGCAAGGCGGCGGUGCCGU 20 4734

HSV2-UL30-375 + GGUGCCGUCUGGGUGGCUCC 20 4735

HSV2-UL30-107 + GUGCCGUCUGGGUGGCUCCC 20 3219

HSV2-UL30-108 + UGCCGUCUGGGUGGCUCCCC 20 3220

HSV2-UL30-378 + UGGGUGGCUCCCCGGGGGUU 20 4736

HSV2-UL30-111 + GGGUGGCUCCCCGGGGGUUG 20 3222

HSV2-UL30-112 + GGUGGCUCCCCGGGGGUUGU 20 3223

HSV2-UL30-113 + GUGGCUCCCCGGGGGUUGUG 20 3224 HSV2-UL30-382 + GCAAAAAACCCAGACGCCGC 20 4737

HSV2-UL30-383 + CCCGAGCCGCCGGCUUCCCC 20 4738

HSV2-UL30-117 + CCGAGCCGCCGGCUUCCCCC 20 3228

HSV2-UL30-118 + CGAGCCGCCGGCUUCCCCCC 20 3229

HSV2-UL30-119 + GAGCCGCCGGCU UCCCCCCG 20 3230

HSV2-UL30-120 + AGCCGCCGGCUUCCCCCCGG 20 3231

HSV2-UL30-388 + CGGCUUCCCCCCGGGGGAAG 20 4739

HSV2-UL30-389 + GCGUGUUCCACGUGCUC 17 4740

HSV2-UL30-390 + UCCAGGAUGUCGUACAC 17 4741

HSV2-UL30-179 + CCAGGAUGUCGUACACG 17 3272

HSV2-UL30-392 + CGUGGAAGACGGUGACG 17 4742

HSV2-UL30-182 + GUGGAAGACGGUGACGG 17 3275

HSV2-UL30-394 + AGACGGUGACGGUGGGG 17 4743

HSV2-UL30-395 + CGGUGGGGUCGAACCCC 17 4744

HSV2-UL30-185 + GGUGGGGUCGAACCCCU 17 3131

HSV2-UL30-186 + GUGGGGUCGAACCCCUC 17 3132

HSV2-UL30-398 + GGCGCAAGCGACGCGGC 17 4745

HSV2-UL30-399 + ACGCGGCCAGAAGCCCU 17 4746

HSV2-UL30-400 + AAGCCCUCCGGGCCCAC 17 4747

HSV2-UL30-194 + AGCCCUCCGGGCCCACG 17 3279

HSV2-UL30-402 + CCCUCCGGGCCCACGCG 17 4748

HSV2-UL30-403 + CCCCCCGCAGUACACCU 17 4749

HSV2-UL30-196 + CCCCCGCAGUACACCUU 17 3281

HSV2-UL30-405 + UACACCUUAGGGGCGCG 17 4750

HSV2-UL30-199 + ACACCUUAGGGGCGCGC 17 3140

HSV2-UL30-407 + CGCCGGAGGCGGCCGUC 17 4751

HSV2-UL30-408 + CCCCGGUGCGCUGCUCC 17 4752

HSV2-UL30-204 + CCCGGUGCGCUGCUCCG 17 3285

HSV2-UL30-205 + CCGGUGCGCUGCUCCGC 17 3286

HSV2-UL30-411 + GGGGGCGUCCUCGUCCA 17 4753

HSV2-UL30-412 + GUCCUCGUCCAGCGAAC 17 4754

HSV2-UL30-208 + U CCU CG U CCAGCG AACG 17 3143

HSV2-UL30-414 + GAACGCGGGGCGAUAAA 17 4755

HSV2-UL30-415 + CUGUAGUACGUAUGGCG 17 4756

HSV2-UL30-416 + UAUGGCGCUGAGCCGGC 17 4757

HSV2-UL30-417 + UGGCGCUGAGCCGGCCC 17 4758

HSV2-UL30-418 + UUUGGCUGCGUUCCGGU 17 4759

HSV2-UL30-419 + GCUGCGUUCCGGUCUGA 17 4760

HSV2-UL30-420 + GUUCCGGUCUGAGCGAG 17 4761

HSV2-UL30-218 + UUCCGGUCUGAGCGAGG 17 3149

HSV2-UL30-219 + UCCGGUCUGAGCGAGGU 17 3292

HSV2-UL30-423 + GAGCGAGGUGGGGGUUG 17 4762

HSV2-UL30-424 + CAAGGCGGCGGUGCCGU 17 4763

HSV2-UL30-425 + GCCGUCUGGGUGGCUCC 17 4764

HSV2-UL30-230 + CCGUCUGGGUGGCUCCC 17 3297

HSV2-UL30-231 + CGUCUGGGUGGCUCCCC 17 3298 HSV2-UL30-428 + GUGGCUCCCCGGGGGUU 17 4765

HSV2-UL30-234 + UGGCUCCCCGGGGGUUG 17 3300

HSV2-UL30-235 + GGCUCCCCGGGGGUUGU 17 3301

HSV2-UL30-236 + GCUCCCCGGGGGU UGUG 17 3302

HSV2-UL30-432 + AAAAACCCAGACGCCGC 17 4766

HSV2-UL30-433 + GAGCCGCCGGCUUCCCC 17 4767

HSV2-UL30-240 + AGCCGCCGGCUUCCCCC 17 3306

HSV2-UL30-241 + GCCGCCGGCUUCCCCCC 17 3307

HSV2-UL30-242 + CCGCCGGCUUCCCCCCG 17 3308

HSV2-UL30-243 + CGCCGGCUUCCCCCCGG 17 3309

HSV2-UL30-438 + CUUCCCCCCGGGGGAAG 17 4768

Table 2E provides exemplary targeting domains for knocking out the UL30 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of the UL30 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2E

HSV2-UL30-458 - GGCGGCCCUGCGCGAGUCGC 20 3328

HSV2-UL30-459 - GCGGCCCUGCGCGAGUCGCC 20 3329

HSV2-UL30-1804 - CGUCGUUCCGCGGCAUCUCC 20 4778

HSV2-UL30-1805 - CGGCAUCUCCGCGGACCACU 20 4779

HSV2-UL30-1806 - UCUCCGCGGACCACUUCGAG 20 4780

HSV2-UL30-465 - CUCCGCGGACCACUUCGAGG 20 3335

HSV2-UL30-1808 - ACCACUUCGAGGCGGAGGUG 20 4781

HSV2-UL30-468 - CCACUUCGAGGCGGAGGUGG 20 3338

HSV2-UL30-1810 - GCGCGCCGACGUGUACUAUU 20 4782

HSV2-UL30-1811 - CUGUACUACCGCGUCUUCGU 20 4783

HSV2-UL30-1812 - CUACCGCGUCUUCGUGCGAA 20 4784

HSV2-UL30-1813 - GACAACU U U UGCCCCGCGAU 20 4785

HSV2-UL30-472 - ACAACUUUUGCCCCGCGAUC 20 3342

HSV2-UL30-1815 - UUGCCCCGCGAUCAGGAAGU 20 4786

HSV2-UL30-1816 - GCCCCGCGAUCAGGAAGUAC 20 4787

HSV2-UL30-473 - CCCCGCGAUCAGGAAGUACG 20 3343

HSV2-UL30-474 - CCCGCGAUCAGGAAGUACGA 20 3344

HSV2-UL30-475 - CCGCGAUCAGGAAGUACGAG 20 3345

HSV2-UL30-1820 - ACGCCACCACCCGGU U U AUC 20 4788

HSV2-UL30-1821 - CCCGGUU UAUCCUGGACAAC 20 4789

HSV2-UL30-480 - CCGGU UUAUCCUGGACAACC 20 3350

HSV2-UL30-1823 - GUACCGCCUCAAGCCCGGCC 20 4790

HSV2-UL30-486 - UACCGCCUCAAGCCCGGCCG 20 3356

HSV2-UL30-487 - ACCGCCUCAAGCCCGGCCGC 20 3357

HSV2-UL30-1826 - ACCGCGCCCCCCGACGGCGU 20 4791

HSV2-UL30-490 - CCGCGCCCCCCGACGGCGUU 20 3360

HSV2-UL30-1828 - CCCCG ACGG CG U U CGGAACC 20 4792

HSV2-UL30-1829 - GUUCGGAACCUCGAGCGACG 20 4793

HSV2-UL30-1830 - ACGUCGAGUUUAACUGCACG 20 4794

HSV2-UL30-1831 - CACGGCGGACAACCUGGCCG 20 4795

HSV2-UL30-1832 - CGGCGGACAACCUGGCCGUC 20 4796

HSV2-UL30-494 - GGCGGACAACCUGGCCGUCG 20 3364

HSV2-UL30-495 - GCGGACAACCUGGCCGUCGA 20 3365

HSV2-UL30-1835 - CAAGCUCAUGUGCUUCGAUA 20 4797

HSV2-UL30-1836 - CUUCGAUAUCGAAUGCAAGG 20 4798

HSV2-UL30-500 - UUCGAUAUCGAAUGCAAGGC 20 3370

HSV2-UL30-501 - UCGAUAUCGAAUGCAAGGCC 20 3371

HSV2-UL30-502 - CGAUAUCGAAUGCAAGGCCG 20 3372

HSV2-UL30-503 - GAUAUCGAAUGCAAGGCCGG 20 3373

HSV2-UL30-504 - AUAUCGAAUGCAAGGCCGGG 20 3374

HSV2-UL30-505 - UAUCGAAUGCAAGGCCGGGG 20 3375

HSV2-UL30-1843 - UCGAAUGCAAGGCCGGGGGG 20 4799

HSV2-UL30-1844 - AUGCAAGGCCGGGGGGGAGG 20 4800

HSV2-UL30-1845 - ACGAGCUGGCCUUUCCGGUC 20 4801

HSV2-UL30-509 - CGAGCUGGCCUUUCCGGUCG 20 3379

HSV2-UL30-1847 - CCUUUCCGGUCGCGGAACGC 20 4802 HSV2-UL30-510 - CUUUCCGGUCGCGGAACGCC 20 3380

HSV2-UL30-1849 - CG ACCU G U CCACCACCG CCC 20 4803

HSV2-UL30-1850 - GCACAUCCUCCUGUUUUCGC 20 4804

HSV2-UL30-1851 - GCUCGGAUCCUGCGACCUCC 20 4805

HSV2-UL30-1852 - CACCUCAGCGAUCUCGCCUC 20 4806

HSV2-UL30-512 - ACCUCAGCGAUCUCGCCUCC 20 3382

HSV2-UL30-1854 - GCCUGCCGGCCCCCGUCGUC 20 4807

HSV2-UL30-516 - CCUGCCGGCCCCCGUCGUCC 20 3386

HSV2-UL30-1856 - CGUCGUCCUGGAGUUUGACA 20 4808

HSV2-UL30-1857 - CCUGGAGUUUGACAGCGAAU 20 4809

HSV2-UL30-1858 - CUUCGUCAAGCAGUACGGCC 20 4810

HSV2-UL30-1859 - GUACGGCCCCGAGUUCGUGA 20 4811

HSV2-UL30-1860 - CCUUCGUCCUGACCAAGCUG 20 4812

HSV2-UL30-522 - CUUCGUCCUGACCAAGCUGA 20 3392

HSV2-UL30-1862 - GAUCUACAAGGUCCCGCUCG 20 4813

HSV2-UL30-1863 - CAAGGUCCCGCUCGACGGGU 20 4814

HSV2-UL30-1864 - CGCUCGACGGGUACGGGCGC 20 4815

HSV2-UL30-1865 - GGGUACGGGCGCAUGAACGG 20 4816

HSV2-UL30-529 - GGUACGGGCGCAUGAACGGC 20 3399

HSV2-UL30-1867 - GGCCGGGGUGUGUUCCGCGU 20 4817

HSV2-UL30-532 - GCCGGGGUGUGUUCCGCGUG 20 3402

HSV2-UL30-1869 - UCCGCGUGUGGGACAUCGGC 20 4818

HSV2-UL30-1870 - ACAUCGGCCAGAGCCACUUU 20 4819

HSV2-UL30-1871 - AGAAGCGCAGCAAGAUCAAG 20 4820

HSV2-UL30-1872 - GCGCAGCAAGAUCAAGGUGA 20 4821

HSV2-UL30-536 - CGCAGCAAGAUCAAGGUGAA 20 3406

HSV2-UL30-1874 - AGAUCAAGGUGAACGGGAUG 20 4822

HSV2-UL30-1875 - UCAAACUCUCCAGCUACAAG 20 4823

HSV2-UL30-1876 - CUACAAGCUGAACGCCGUCG 20 4824

HSV2-UL30-1877 - ACGCCGUCGCCGAGGCCGUC 20 4825

HSV2-UL30-1878 - CCGUCGCCGAGGCCGUCUUG 20 4826

HSV2-UL30-1879 - CCGAGGCCGUCUUGAAGGAC 20 4827

HSV2-UL30-1880 - AGGCCGUCUUGAAGGACAAG 20 4828

HSV2-UL30-1881 - CCG U CU U G AAGG ACAAG AAG 20 4829

HSV2-UL30-1882 - UGAAGGACAAGAAGAAGGAU 20 4830

HSV2-UL30-1883 - CAUCCCCGCCUACUACGCCU 20 4831

HSV2-UL30-1884 - CGCCUCCGGGCCCGCGCAGC 20 4832

HSV2-UL30-546 - GCCUCCGGGCCCGCGCAGCG 20 3416

HSV2-UL30-1886 - CGCGCAGCGCGGGGUGAUCG 20 4833

HSV2-UL30-1887 - UGAUCGGCGAGUAUUGUGUG 20 4834

HSV2-UL30-1888 - UGUGCAGGACUCGCUGCUGG 20 4835

HSV2-UL30-1889 - UCUUCAAGUUUCUGCCGCAC 20 4836

HSV2-UL30-554 - CUUCAAGUUUCUGCCGCACC 20 3424

HSV2-UL30-1891 - UUUCCGCCGUCGCGCGCCUG 20 4837

HSV2-UL30-1892 - UCACGUGCCUCCUGCGCCUU 20 4838

HSV2-UL30-1893 - GCCUCCUGCGCCUUGCGGGC 20 4839 HSV2-UL30-1894 - UCCUGCGCCUUGCGGGCCAG 20 4840

HSV2-UL30-1895 - GCCAGAAGGGCUUCAUCCUG 20 4841

HSV2-UL30-1896 - GCU UCAUCCUGCCGGACACC 20 4842

HSV2-UL30-564 - CU UCAUCCUGCCGGACACCC 20 3434

HSV2-UL30-1898 - CCGGACACCCAGGGGCGGUU 20 4843

HSV2-UL30-568 - CGGACACCCAGGGGCGGUUU 20 3438

HSV2-UL30-1900 - GGCGGUUUCGGGGCCUCGAC 20 4844

HSV2-UL30-571 - GCGGUUUCGGGGCCUCGACA 20 3441

HSV2-UL30-1902 - CCCAAGCGCCCGGCCGUGCC 20 4845

HSV2-UL30-574 - CCAAGCGCCCGGCCGUGCCU 20 3444

HSV2-UL30-575 - CAAGCGCCCGGCCGUGCCUC 20 3445

HSV2-UL30-576 - AAGCGCCCGGCCGUGCCUCG 20 3446

HSV2-UL30-577 - AGCGCCCGGCCGUGCCUCGG 20 3447

HSV2-UL30-578 - GCGCCCGGCCGUGCCUCGGG 20 3448

HSV2-UL30-1908 - CCCGGCCGUGCCUCGGGGGG 20 4846

HSV2-UL30-579 - CCGGCCGUGCCUCGGGGGGA 20 3449

HSV2-UL30-580 - CGGCCGUGCCUCGGGGGGAA 20 3450

HSV2-UL30-581 - GGCCGUGCCUCGGGGGGAAG 20 3451

HSV2-UL30-1912 - CUCGGGGGGAAGGGGAGCGG 20 4847

HSV2-UL30-583 - UCGGGGGGAAGGGGAGCGGC 20 3453

HSV2-UL30-584 - CGGGGGGAAGGGGAGCGGCC 20 3454

HSV2-UL30-585 - GGGGGGAAGGGGAGCGGCCG 20 3455

HSV2-UL30-1916 - GGAAGGGGAGCGGCCGGGGG 20 4848

HSV2-UL30-587 - GAAGGGGAGCGGCCGGGGGA 20 3457

HSV2-UL30-588 - AAGGGGAGCGGCCGGGGGAC 20 3458

HSV2-UL30-1919 - GGAGCGGCCGGGGGACGGGA 20 4849

HSV2-UL30-589 - GAGCGGCCGGGGGACGGGAA 20 3459

HSV2-UL30-590 - AGCGGCCGGGGGACGGGAAC 20 3460

HSV2-UL30-1922 - GCCGGGGGACGGGAACGGGG 20 4850

HSV2-UL30-1923 - CGGGGGACGGGAACGGGGAC 20 4851

HSV2-UL30-1924 - ACGGGAACGGGGACGAGGAU 20 4852

HSV2-UL30-1925 - GGACGAGGAUAAGGACGACG 20 4853

HSV2-UL30-1926 - ACGAGGAUAAGGACGACGAC 20 4854

HSV2-UL30-1927 - GGAUAAGGACGACGACGAGG 20 4855

HSV2-UL30-595 - GAUAAGGACGACGACGAGGA 20 3465

HSV2-UL30-596 - AUAAGGACGACGACGAGGAC 20 3466

HSV2-UL30-1930 - GGACGACGACGAGGACGGGG 20 4856

HSV2-UL30-1931 - ACGACGACGAGGACGGGGAC 20 4857

HSV2-UL30-1932 - CGACGAGGACGGGGACGAGG 20 4858

HSV2-UL30-599 - GACGAGGACGGGGACGAGGA 20 3469

HSV2-UL30-600 - ACGAGGACGGGGACGAGGAC 20 3470

HSV2-UL30-1935 - GGACGGGGACGAGGACGGGG 20 4859

HSV2-UL30-1936 - GGACGAGGACGGGGACGAGC 20 4860

HSV2-UL30-1937 - ACGAGGACGGGGACGAGCGC 20 4861

HSV2-UL30-602 - CGAGGACGGGGACGAGCGCG 20 3472

HSV2-UL30-1939 - CGAGCGCGAGGAGGUCGCGC 20 4862 HSV2-UL30-1940 - CGAGGAGGUCGCGCGCGAGA 20 4863

HSV2-UL30-604 - GAGGAGGUCGCGCGCGAGAC 20 3474

HSV2-UL30-605 - AGGAGGUCGCGCGCGAGACC 20 3475

HSV2-UL30-1943 - CGAGACCGGGGGCCGGCACG 20 4864

HSV2-UL30-1944 - GGGGCCGGCACGUUGGGUAC 20 4865

HSV2-UL30-611 - GGGCCGGCACGUUGGGUACC 20 3481

HSV2-UL30-612 - GGCCGGCACGUUGGGUACCA 20 3482

HSV2-UL30-1947 - CACGUUGGGUACCAGGGGGC 20 4866

HSV2-UL30-1948 - CCG GG U CCU CG ACCCCACCU 20 4867

HSV2-UL30-1949 - CAGUACGCUCUCCCUGCGGC 20 4868

HSV2-UL30-1950 - GGCCCGAGGCCGUCGCGCAC 20 4869

HSV2-UL30-624 - GCCCGAGGCCGUCGCGCACC 20 3494

HSV2-UL30-1952 - AGGCCGUCGCGCACCUGGAG 20 4870

HSV2-UL30-1953 - GUCGCGCACCUGGAGGCGGA 20 4871

HSV2-UL30-627 - UCGCGCACCUGGAGGCGGAC 20 3497

HSV2-UL30-1955 - UGGAGGCGGACCGGGACUAC 20 4872

HSV2-UL30-629 - GGAGGCGGACCGGGACUACC 20 3499

HSV2-UL30-1957 - GGACCGGGACUACCUGGAGA 20 4873

HSV2-UL30-1958 - GGGACUACCUGGAGAUCGAG 20 4874

HSV2-UL30-631 - GGACUACCUGGAGAUCGAGG 20 3501

HSV2-UL30-632 - GACUACCUGGAGAUCGAGGU 20 3502

HSV2-UL30-633 - ACUACCUGGAGAUCGAGGUG 20 3503

HSV2-UL30-1962 - GGGGCCGACGGCUGUUCUUC 20 4875

HSV2-UL30-1963 - CUUCGUGAAGGCCCACGUAC 20 4876

HSV2-UL30-1964 - UCGUGAAGGCCCACGUACGC 20 4877

HSV2-UL30-1965 - CCCACGUACGCGAGAGCCUG 20 4878

HSV2-UL30-1966 - CUGCGCGACUGGCUGGCCAU 20 4879

HSV2-UL30-1967 - AUGCGAAAGCAGAUCCGCUC 20 4880

HSV2-UL30-1968 - AGAUCCGCUCGCGGAUCCCC 20 4881

HSV2-UL30-1969 - GCGGAUCCCCCAGAGCACCC 20 4882

HSV2-UL30-1970 - GGAUCCCCCAGAGCACCCCC 20 4883

HSV2-UL30-641 - GAUCCCCCAGAGCACCCCCG 20 3511

HSV2-UL30-1972 - GGUGGUGUGCAACUCGGUGU 20 4884

HSV2-UL30-1973 - CAACUCGGUGUACGGGUUCA 20 4885

HSV2-UL30-649 - AACUCGGUGUACGGGUUCAC 20 3519

HSV2-UL30-1975 - CACCGUGACGACCAUCGGCC 20 4886

HSV2-UL30-1976 - CGCGCGUACGUGCACGCGCG 20 4887

HSV2-UL30-1977 - CGUACGUGCACGCGCGCUGG 20 4888

HSV2-UL30-657 - GUACGUGCACGCGCGCUGGG 20 3527

HSV2-UL30-1979 - AUCAGCUGCUGGCCGACUUU 20 4889

HSV2-UL30-659 - UCAGCUGCUGGCCGACUUUC 20 3529

HSV2-UL30-1981 - GUACUCCAUGCGCAUCAUCU 20 4890

HSV2-UL30-664 - UACUCCAUGCGCAUCAUCUA 20 3534

HSV2-UL30-665 - ACUCCAUGCGCAUCAUCUAC 20 3535

HSV2-UL30-1984 - UGCGCAUCAUCUACGGGGAC 20 4891

HSV2-UL30-1985 - UGUGCCGCGGCCUCACGGCC 20 4892 HSV2-UL30-1986 - CGGCCGCGGGCCUGGUGGCC 20 4893

HSV2-UL30-1987 - UGGCCAUGGGCGACAAGAUG 20 4894

HSV2-UL30-1988 - GUUCCUCCCCCCGAUCAAGC 20 4895

HSV2-UL30-1989 - CCCCCCGAUCAAGCUCGAGU 20 4896

HSV2-UL30-1990 - CCAAGCUGCUGCUCAUCGCC 20 4897

HSV2-UL30-1991 - AAAGUACAUCGGCGUCAUCU 20 4898

HSV2-UL30-678 - AAGUACAUCGGCGUCAUCUG 20 3548

HSV2-UL30-679 - AGUACAUCGGCGUCAUCUGC 20 3549

HSV2-UL30-1994 - GCGGGGGCAAGAUGCUCAUC 20 4899

HSV2-UL30-1995 - GCAAGAUGCUCAUCAAGGGC 20 4900

HSV2-UL30-1996 - GCGUUUAUCAACCGCACCUC 20 4901

HSV2-UL30-1997 - GUUUUACGACGAUACCGUAU 20 4902

HSV2-UL30-689 - UUUUACGACGAUACCGUAUC 20 3559

HSV2-UL30-1999 - CGGAGCGGCCGCCGCGUUAG 20 4903

HSV2-UL30-2000 - CGCGU UAGCCGAGCGCCCCG 20 4904

HSV2-UL30-2001 - CGUUAGCCGAGCGCCCCGCA 20 4905

HSV2-UL30-691 - GUUAGCCGAGCGCCCCGCAG 20 3561

HSV2-UL30-2003 - GUGGCUGGCGCGACCCCUGC 20 4906

HSV2-UL30-2004 - GGCUGGCGCGACCCCUGCCC 20 4907

HSV2-UL30-694 - GCUGGCGCGACCCCUGCCCG 20 3564

HSV2-UL30-2006 - GCCCGAGGGACUGCAGGCGU 20 4908

HSV2-UL30-697 - CCCGAGGGACUGCAGGCGUU 20 3567

HSV2-UL30-2008 - CCCAUCGGCG CAU CACCG AC 20 4909

HSV2-UL30-701 - CCAUCGGCGCAUCACCGACC 20 3571

HSV2-UL30-2010 - AUCGGCGCAUCACCGACCCG 20 4910

HSV2-UL30-2011 - CGGCGCAUCACCGACCCGGA 20 4911

HSV2-UL30-702 - GGCGCAUCACCGACCCGGAG 20 3572

HSV2-UL30-2013 - CCGACCCGGAGAGGGACAUC 20 4912

HSV2-UL30-2014 - CCAG G ACU U U G U CCU CACCG 20 4913

HSV2-UL30-2015 - ACUUUGUCCUCACCGCCGAA 20 4914

HSV2-UL30-2016 - GCGCGCAGGUCCCGUCCAUC 20 4915

HSV2-UL30-2017 - CAGGUCCCGUCCAUCAAGGA 20 4916

HSV2-UL30-2018 - CGUGAUCGUGGCCCAGACCC 20 4917

HSV2-UL30-2019 - CGUGGCCCAGACCCGCGAGG 20 4918

HSV2-UL30-2020 - UGGCCCAGACCCGCGAGGUA 20 4919

HSV2-UL30-713 - GGCCCAGACCCGCGAGGUAG 20 3583

HSV2-UL30-2022 - CGCGCGGCUGGCCGCCCUCC 20 4920

HSV2-UL30-2023 - CGAGCUAGACGCCGCCGCCC 20 4921

HSV2-UL30-717 - GAGCUAGACGCCGCCGCCCC 20 3587

HSV2-UL30-718 - AGCUAGACGCCGCCGCCCCA 20 3588

HSV2-UL30-2026 - AGACGCCGCCGCCCCAGGGG 20 4922

HSV2-UL30-2027 - CCCUCCCCGGCCAAGCGCCC 20 4923

HSV2-UL30-722 - CCUCCCCGGCCAAGCGCCCC 20 3592

HSV2-UL30-723 - CUCCCCGGCCAAGCGCCCCC 20 3593

HSV2-UL30-2030 - CGCCGUCGCAUGCCGACCCC 20 4924

HSV2-UL30-724 - G CCG U CG CAU GCCG ACCCCC 20 3594 HSV2-UL30-725 - CCGUCGCAUGCCGACCCCCC 20 3595

HSV2-UL30-2033 - GCCCCGCAAGCUGCUGGUGU 20 4925

HSV2-UL30-2034 - AGCUGCUGGUGUCCGAGCUG 20 4926

HSV2-UL30-729 - GCUGCUGGUGUCCGAGCUGG 20 3599

HSV2-UL30-2036 - UGCUGGUGUCCGAGCUGGCG 20 4927

HSV2-UL30-2037 - GUCCGAGCUGGCGGAGGAUC 20 4928

HSV2-UL30-2038 - GAUCCCGGGUACGCCAUCGC 20 4929

HSV2-UL30-733 - AUCCCGGGUACGCCAUCGCC 20 3603

HSV2-UL30-2040 - CCCGGGGCGUUCCGCUCAAC 20 4930

HSV2-UL30-2041 - ACUAUUACUUCUCGCACCUG 20 4931

HSV2-UL30-737 - CUAUUACUUCUCGCACCUGC 20 3607

HSV2-UL30-738 - UAUUACUUCUCGCACCUGCU 20 3608

HSV2-UL30-2044 - CGUGACGUUCAAGGCCCUGU 20 4932

HSV2-UL30-743 - GUGACGUUCAAGGCCCUGUU 20 3613

HSV2-UL30-2046 - UGGAAAUAACGCCAAGAUCA 20 4933

HSV2-UL30-2047 - GAAAUAACG CCAAG AU CACC 20 4934

HSV2-UL30-2048 - AGAUCACCGAGAGUCUGUUA 20 4935

HSV2-UL30-2049 - UCUGUUAAAGAGGUUUAUUC 20 4936

HSV2-UL30-2050 - U UCCCGAGACG UGGCACCCC 20 4937

HSV2-UL30-2051 - GACGACGUGGCCGCGCGGCU 20 4938

HSV2-UL30-2052 - UGGCCGCGCGGCUCAGGGCC 20 4939

HSV2-UL30-751 - GGCCGCGCGGCUCAGGGCCG 20 3621

HSV2-UL30-2054 - GCGGCUCAGGGCCGCGGGGU 20 4940

HSV2-UL30-2055 - GGGCCGCGGGGUUCGGGCCG 20 4941

HSV2-UL30-757 - GGCCGCGGGGUUCGGGCCGG 20 3627

HSV2-UL30-758 - GCCGCGGGGUUCGGGCCGGC 20 3628

HSV2-UL30-2058 - CGGCGGGGGCCGGCGCUACG 20 4942

HSV2-UL30-763 - GGCGGGGGCCGGCGCUACGG 20 3633

HSV2-UL30-2060 - CGGGGGCCGGCGCUACGGCG 20 4943

HSV2-UL30-764 - GGGGGCCGGCGCUACGGCGG 20 3634

HSV2-UL30-2062 - GCUACGGCGGAGGAAACUCG 20 4944

HSV2-UL30-2063 - GAAACUCGUCGAAUGUUGCA 20 4945

HSV2-UL30-2064 - GGACGCCAUCACGCCCG 17 4946

HSV2-UL30-765 - GACGCCAUCACGCCCGC 17 3635

HSV2-UL30-2066 - GGACCGUCAUCACGCUU 17 4947

HSV2-UL30-2067 - GCU UCUGGGUCUGACCC 17 4948

HSV2-UL30-2068 - CGCGGCAGUACUUUUAC 17 4949

HSV2-UL30-2069 - ACU U U U ACAU G AACAAG 17 4950

HSV2-UL30-773 - CUUUUACAUGAACAAGG 17 3643

HSV2-UL30-2071 - ACAUGAACAAGGCGGAG 17 4951

HSV2-UL30-2072 - UGCCCCGCGCGAUCUCU 17 4952

HSV2-UL30-2073 - CCUGGCGGCGGCCCUGC 17 4953

HSV2-UL30-2074 - CGGCCCUGCGCGAGUCG 17 4954

HSV2-UL30-780 - GGCCCUGCGCGAGUCGC 17 3650

HSV2-UL30-781 - GCCCUGCGCGAGUCGCC 17 3651

HSV2-UL30-2077 - CGUUCCGCGGCAUCUCC 17 4955 HSV2-UL30-2078 - CAUCUCCGCGGACCACU 17 4956

HSV2-UL30-2079 - CCGCGGACCACU UCGAG 17 4957

HSV2-UL30-787 - CGCGGACCACU UCGAGG 17 3657

HSV2-UL30-2081 - ACUUCGAGGCGGAGGUG 17 4958

HSV2-UL30-790 - CU UCGAGGCGGAGGUGG 17 3660

HSV2-UL30-2083 - CGCCGACGUGUACUAUU 17 4959

HSV2-UL30-2084 - UACUACCGCGUCUUCGU 17 4960

HSV2-UL30-2085 - CCGCGUCUUCGUGCGAA 17 4961

HSV2-UL30-2086 - AACUUUUGCCCCGCGAU 17 4962

HSV2-UL30-794 - ACUUUUGCCCCGCGAUC 17 3664

HSV2-UL30-2088 - CCCCG CG AU CAG G AAG U 17 4963

HSV2-UL30-2089 - CCG CG AU CAG G AAG U AC 17 4964

HSV2-UL30-795 - CGCGAUCAGGAAGUACG 17 3665

HSV2-UL30-796 - GCGAUCAGGAAGUACGA 17 3666

HSV2-UL30-797 - CGAUCAGGAAGUACGAG 17 3667

HSV2-UL30-2093 - CCACCACCCG G U U U AU C 17 4965

HSV2-UL30-2094 - GGUUUAUCCUGGACAAC 17 4966

HSV2-UL30-802 - GUUUAUCCUGGACAACC 17 3672

HSV2-UL30-2096 - CCGCCUCAAGCCCGGCC 17 4967

HSV2-UL30-808 - CGCCUCAAGCCCGGCCG 17 3678

HSV2-UL30-809 - GCCUCAAGCCCGGCCGC 17 3679

HSV2-UL30-2099 - GCGCCCCCCGACGGCGU 17 4968

HSV2-UL30-812 - CGCCCCCCGACGGCGUU 17 3682

HSV2-UL30-2101 - CGACGGCGUUCGGAACC 17 4969

HSV2-UL30-2102 - CGGAACCUCGAGCGACG 17 4970

HSV2-UL30-2103 - UCGAGUUUAACUGCACG 17 4971

HSV2-UL30-2104 - GGCGGACAACCUGGCCG 17 4972

HSV2-UL30-2105 - CG G ACAACCU G GCCG U C 17 4973

HSV2-UL30-816 - GGACAACCUGGCCGUCG 17 3686

HSV2-UL30-817 - GACAACCUGGCCGUCGA 17 3687

HSV2-UL30-2108 - GCUCAUGUGCUUCGAUA 17 4974

HSV2-UL30-2109 - CGAUAUCGAAUGCAAGG 17 4975

HSV2-UL30-822 - GAUAUCGAAUGCAAGGC 17 3692

HSV2-UL30-823 - AUAUCGAAUGCAAGGCC 17 3693

HSV2-UL30-824 - UAUCGAAUGCAAGGCCG 17 3694

HSV2-UL30-825 - AUCGAAUGCAAGGCCGG 17 3695

HSV2-UL30-826 - UCGAAUGCAAGGCCGGG 17 3696

HSV2-UL30-827 - CGAAUGCAAGGCCGGGG 17 3697

HSV2-UL30-2116 - AAUGCAAGGCCGGGGGG 17 4976

HSV2-UL30-2117 - CAAGGCCGGGGGGGAGG 17 4977

HSV2-UL30-2118 - AGCUGGCCUUUCCGGUC 17 4978

HSV2-UL30-831 - GCUGGCCUUUCCGGUCG 17 3701

HSV2-UL30-2120 - UUCCGGUCGCGGAACGC 17 4979

HSV2-UL30-832 - UCCGGUCGCGGAACGCC 17 3702

HSV2-UL30-2122 - CCUGUCCACCACCGCCC 17 4980

HSV2-UL30-2123 - CAUCCUCCUGUUUUCGC 17 4981 HSV2-UL30-2124 - CGGAUCCUGCGACCUCC 17 4982

HSV2-UL30-2125 - CUCAGCGAUCUCGCCUC 17 4983

HSV2-UL30-834 - UCAGCGAUCUCGCCUCC 17 3704

HSV2-UL30-2127 - UGCCGGCCCCCGUCGUC 17 4984

HSV2-UL30-838 - GCCGGCCCCCGUCGUCC 17 3708

HSV2-UL30-2129 - CGUCCUGGAGUUUGACA 17 4985

HSV2-UL30-2130 - GGAGUUUGACAGCGAAU 17 4986

HSV2-UL30-2131 - CGUCAAGCAGUACGGCC 17 4987

HSV2-UL30-2132 - CGGCCCCGAGUUCGUGA 17 4988

HSV2-UL30-2133 - UCGUCCUGACCAAGCUG 17 4989

HSV2-UL30-844 - CGUCCUGACCAAGCUGA 17 3714

HSV2-UL30-2135 - CU AC AAG GUCCCGCUCG 17 4990

HSV2-UL30-2136 - GGUCCCGCUCGACGGGU 17 4991

HSV2-UL30-2137 - UCGACGGGUACGGGCGC 17 4992

HSV2-UL30-2138 - UACGGGCGCAUGAACGG 17 4993

HSV2-UL30-851 - ACGGGCGCAUGAACGGC 17 3721

HSV2-UL30-2140 - CGGGGUGUGUUCCGCGU 17 4994

HSV2-UL30-854 - GGGGUGUGUUCCGCGUG 17 3724

HSV2-UL30-2142 - GCGUGUGGGACAUCGGC 17 4995

HSV2-UL30-2143 - UCGGCCAGAGCCACUUU 17 4996

HSV2-UL30-2144 - AGCGCAGCAAGAUCAAG 17 4997

HSV2-UL30-2145 - CAGCAAGAUCAAGGUGA 17 4998

HSV2-UL30-858 - AG CAAG AU CAAG G U G AA 17 3728

HSV2-UL30-2147 - UCAAGGUGAACGGGAUG 17 4999

HSV2-UL30-2148 - AACUCU CCAG CU ACAAG 17 5000

HSV2-UL30-2149 - CAAGCUGAACGCCGUCG 17 5001

HSV2-UL30-2150 - CCGUCGCCGAGGCCGUC 17 5002

HSV2-UL30-2151 - UCGCCGAGGCCGUCUUG 17 5003

HSV2-UL30-2152 - AGGCCGUCUUGAAGGAC 17 5004

HSV2-UL30-2153 - CCGUCU UGAAGGACAAG 17 5005

HSV2-UL30-2154 - UCUUGAAGGACAAGAAG 17 5006

HSV2-UL30-2155 - AGGACAAGAAGAAGGAU 17 5007

HSV2-UL30-2156 - CCCCGCCUACUACGCCU 17 5008

HSV2-UL30-2157 - CUCCGGGCCCGCGCAGC 17 5009

HSV2-UL30-868 - UCCGGGCCCGCGCAGCG 17 3738

HSV2-UL30-2159 - GCAGCGCGGGGUGAUCG 17 5010

HSV2-UL30-2160 - UCGGCGAGUAUUGUGUG 17 5011

HSV2-UL30-2161 - GCAGGACUCGCUGCUGG 17 5012

HSV2-UL30-2162 - UCAAGUUUCUGCCGCAC 17 5013

HSV2-UL30-876 - CAAGUUUCUGCCGCACC 17 3746

HSV2-UL30-2164 - CCGCCGUCGCGCGCCUG 17 5014

HSV2-UL30-2165 - CGUGCCUCCUGCGCCUU 17 5015

HSV2-UL30-2166 - UCCUGCGCCUUGCGGGC 17 5016

HSV2-UL30-2167 - UGCGCCUUGCGGGCCAG 17 5017

HSV2-UL30-2168 - AGAAGGGCUUCAUCCUG 17 5018

HSV2-UL30-2169 - UCAUCCUGCCGGACACC 17 5019 HSV2-UL30-886 - CAUCCUGCCGGACACCC 17 3756

HSV2-UL30-2171 - GACACCCAGGGGCGGUU 17 5020

HSV2-UL30-890 - ACACCCAGGGGCGGUUU 17 3760

HSV2-UL30-2173 - GGUUUCGGGGCCUCGAC 17 5021

HSV2-UL30-893 - GUUUCGGGGCCUCGACA 17 3763

HSV2-UL30-2175 - AAGCGCCCGGCCGUGCC 17 5022

HSV2-UL30-896 - AGCGCCCGGCCGUGCCU 17 3766

HSV2-UL30-897 - GCGCCCGGCCGUGCCUC 17 3767

HSV2-UL30-898 - CGCCCGGCCGUGCCUCG 17 3768

HSV2-UL30-899 - GCCCGGCCGUGCCUCGG 17 3769

HSV2-UL30-900 - CCCGGCCGUGCCUCGGG 17 3770

HSV2-UL30-2181 - GGCCGUGCCUCGGGGGG 17 5023

HSV2-UL30-901 - GCCGUGCCUCGGGGGGA 17 3771

HSV2-UL30-902 - CCGUGCCUCGGGGGGAA 17 3772

HSV2-UL30-903 - CGUGCCUCGGGGGGAAG 17 3773

HSV2-UL30-2185 - GGGGGGAAGGGGAGCGG 17 5024

HSV2-UL30-905 - GGGGGAAGGGGAGCGGC 17 3775

HSV2-UL30-906 - GGGGAAGGGGAGCGGCC 17 3776

HSV2-UL30-907 - GGGAAGGGGAGCGGCCG 17 3777

HSV2-UL30-2189 - AGGGGAGCGGCCGGGGG 17 5025

HSV2-UL30-909 - GGGGAGCGGCCGGGGGA 17 3779

HSV2-UL30-910 - GGGAGCGGCCGGGGGAC 17 3780

HSV2-UL30-2192 - GCGGCCGGGGGACGGGA 17 5026

HSV2-UL30-911 - CGGCCGGGGGACGGGAA 17 3781

HSV2-UL30-912 - GGCCGGGGGACGGGAAC 17 3782

HSV2-UL30-2195 - GGGGGACGGGAACGGGG 17 5027

HSV2-UL30-2196 - GGGACGGGAACGGGGAC 17 5028

HSV2-UL30-2197 - GGAACGGGGACGAGGAU 17 5029

HSV2-UL30-2198 - CGAGGAUAAGGACGACG 17 5030

HSV2-UL30-2199 - AGGAUAAGGACGACGAC 17 5031

HSV2-UL30-2200 - UAAGGACGACGACGAGG 17 5032

HSV2-UL30-917 - AAGGACGACGACGAGGA 17 3787

HSV2-UL30-918 - AGGACGACGACGAGGAC 17 3788

HSV2-UL30-2203 - CGACGACGAGGACGGGG 17 5033

HSV2-UL30-2204 - ACGACGAGGACGGGGAC 17 5034

HSV2-UL30-2205 - CGAGGACGGGGACGAGG 17 5035

HSV2-UL30-921 - GAGGACGGGGACGAGGA 17 3791

HSV2-UL30-922 - AGGACGGGGACGAGGAC 17 3792

HSV2-UL30-2208 - CGGGGACGAGGACGGGG 17 5036

HSV2-UL30-2209 - CGAGGACGGGGACGAGC 17 5037

HSV2-UL30-2210 - AGGACGGGGACGAGCGC 17 5038

HSV2-UL30-924 - GGACGGGGACGAGCGCG 17 3794

HSV2-UL30-2212 - GCGCGAGGAGGUCGCGC 17 5039

HSV2-UL30-2213 - GGAGGUCGCGCGCGAGA 17 5040

HSV2-UL30-926 - GAGGUCGCGCGCGAGAC 17 3796

HSV2-UL30-927 - AGGUCGCGCGCGAGACC 17 3797 HSV2-UL30-2216 - GACCGGGGGCCGGCACG 17 5041

HSV2-UL30-2217 - GCCGGCACGUUGGGUAC 17 5042

HSV2-UL30-933 - CCGGCACGUUGGGUACC 17 3803

HSV2-UL30-934 - CGGCACGUUGGGUACCA 17 3804

HSV2-UL30-2220 - GUUGGGUACCAGGGGGC 17 5043

HSV2-UL30-2221 - GGUCCUCGACCCCACCU 17 5044

HSV2-UL30-2222 - UACGCUCUCCCUGCGGC 17 5045

HSV2-UL30-2223 - CCGAGGCCGUCGCGCAC 17 5046

HSV2-UL30-946 - CGAGGCCGUCGCGCACC 17 3816

HSV2-UL30-2225 - CCGUCGCGCACCUGGAG 17 5047

HSV2-UL30-2226 - GCGCACCUGGAGGCGGA 17 5048

HSV2-UL30-949 - CGCACCUGGAGGCGGAC 17 3819

HSV2-UL30-2228 - AGGCGGACCGGGACUAC 17 5049

HSV2-UL30-951 - GGCGGACCGGGACUACC 17 3821

HSV2-UL30-2230 - CCGGGACUACCUGGAGA 17 5050

HSV2-UL30-2231 - ACUACCUGGAGAUCGAG 17 5051

HSV2-UL30-953 - CUACCUGGAGAUCGAGG 17 3823

HSV2-UL30-954 - UACCUGGAGAUCGAGGU 17 3824

HSV2-UL30-955 - ACCUGGAGAUCGAGGUG 17 3825

HSV2-UL30-2235 - GCCGACGGCUGUUCUUC 17 5052

HSV2-UL30-2236 - CGUGAAGGCCCACGUAC 17 5053

HSV2-UL30-2237 - UGAAGGCCCACGUACGC 17 5054

HSV2-UL30-2238 - ACGUACGCGAGAGCCUG 17 5055

HSV2-UL30-2239 - CGCGACUGGCUGGCCAU 17 5056

HSV2-UL30-2240 - CGAAAGCAGAUCCGCUC 17 5057

HSV2-UL30-2241 - UCCGCUCGCGGAUCCCC 17 5058

HSV2-UL30-2242 - GAUCCCCCAGAGCACCC 17 5059

HSV2-UL30-2243 - UCCCCCAGAGCACCCCC 17 5060

HSV2-UL30-963 - CCCCCAGAGCACCCCCG 17 3833

HSV2-UL30-2245 - GGUGUGCAACUCGGUGU 17 5061

HSV2-UL30-2246 - CUCGGUGUACGGGUUCA 17 5062

HSV2-UL30-971 - UCGGUGUACGGGUUCAC 17 3841

HSV2-UL30-2248 - CGUGACGACCAUCGGCC 17 5063

HSV2-UL30-2249 - GCGUACGUGCACGCGCG 17 5064

HSV2-UL30-2250 - ACGUGCACGCGCGCUGG 17 5065

HSV2-UL30-979 - CGUGCACGCGCGCUGGG 17 3849

HSV2-UL30-2252 - AGCUGCUGGCCGACUUU 17 5066

HSV2-UL30-981 - GCUGCUGGCCGACUUUC 17 3851

HSV2-UL30-2254 - CUCCAUGCGCAUCAUCU 17 5067

HSV2-UL30-986 - UCCAUGCGCAUCAUCUA 17 3856

HSV2-UL30-987 - CCAUGCGCAUCAUCUAC 17 3857

HSV2-UL30-2257 - GCAUCAUCUACGGGGAC 17 5068

HSV2-UL30-2258 - GCCGCGGCCUCACGGCC 17 5069

HSV2-UL30-2259 - CCGCGGGCCUGGUGGCC 17 5070

HSV2-UL30-2260 - CCAUGGGCGACAAGAUG 17 5071

HSV2-UL30-2261 - CCUCCCCCCGAUCAAGC 17 5072 HSV2-UL30-2262 - CCCGAUCAAGCUCGAGU 17 5073

HSV2-UL30-2263 - AGCUGCUGCUCAUCGCC 17 5074

HSV2-UL30-2264 - GUACAUCGGCGUCAUCU 17 5075

HSV2-UL30-1000 - UACAUCGGCGUCAUCUG 17 3870

HSV2-UL30-1001 - ACAUCGGCGUCAUCUGC 17 3871

HSV2-UL30-2267 - GGGGCAAGAUGCUCAUC 17 5076

HSV2-UL30-2268 - AGAUGCUCAUCAAGGGC 17 5077

HSV2-UL30-2269 - U U U AU CAACCG CACCU C 17 5078

HSV2-UL30-2270 - UUACGACGAUACCGUAU 17 5079

HSV2-UL30-1011 - UACGACGAUACCGUAUC 17 3881

HSV2-UL30-2272 - AGCGGCCGCCGCGUUAG 17 5080

HSV2-UL30-2273 - GUUAGCCGAGCGCCCCG 17 5081

HSV2-UL30-2274 - UAGCCGAGCGCCCCGCA 17 5082

HSV2-UL30-1013 - AGCCGAGCGCCCCGCAG 17 3883

HSV2-UL30-2276 - GCUGGCGCGACCCCUGC 17 5083

HSV2-UL30-2277 - UGGCGCGACCCCUGCCC 17 5084

HSV2-UL30-1016 - GGCGCGACCCCUGCCCG 17 3886

HSV2-UL30-2279 - CGAGGGACUGCAGGCGU 17 5085

HSV2-UL30-1019 - GAGGGACUGCAGGCGUU 17 3889

HSV2-UL30-2281 - AUCGGCGCAUCACCGAC 17 5086

HSV2-UL30-1023 - UCGGCGCAUCACCGACC 17 3893

HSV2-UL30-2283 - GGCGCAUCACCGACCCG 17 5087

HSV2-UL30-2284 - CGCAUCACCGACCCGGA 17 5088

HSV2-UL30-1024 - GCAUCACCGACCCGGAG 17 3894

HSV2-UL30-2286 - ACCCGGAGAGGGACAUC 17 5089

HSV2-UL30-2287 - GGACUUUGUCCUCACCG 17 5090

HSV2-UL30-2288 - U U G U CCU CACCG CCGAA 17 5091

HSV2-UL30-2289 - CGCAGGUCCCGUCCAUC 17 5092

HSV2-UL30-2290 - G U CCCG U CCAUCAAG G A 17 5093

HSV2-UL30-2291 - GAUCGUGGCCCAGACCC 17 5094

HSV2-UL30-2292 - GGCCCAGACCCGCGAGG 17 5095

HSV2-UL30-2293 - CCCAGACCCGCGAGGUA 17 5096

HSV2-UL30-1035 - CCAG ACCCG CG AG G U AG 17 3905

HSV2-UL30-2295 - GCGGCUGGCCGCCCUCC 17 5097

HSV2-UL30-2296 - GCUAGACGCCGCCGCCC 17 5098

HSV2-UL30-1039 - CUAGACGCCGCCGCCCC 17 3909

HSV2-UL30-1040 - UAGACGCCGCCGCCCCA 17 3910

HSV2-UL30-2299 - CGCCGCCGCCCCAGGGG 17 5099

HSV2-UL30-2300 - UCCCCGGCCAAGCGCCC 17 5100

HSV2-UL30-1044 - CCCCGGCCAAGCGCCCC 17 3914

HSV2-UL30-1045 - CCCGGCCAAGCGCCCCC 17 3915

HSV2-UL30-2303 - CGUCGCAUGCCGACCCC 17 5101

HSV2-UL30-1046 - GUCGCAUGCCGACCCCC 17 3916

HSV2-UL30-1047 - UCGCAUGCCGACCCCCC 17 3917

HSV2-UL30-2306 - CCGCAAGCUGCUGGUGU 17 5102

HSV2-UL30-2307 - UGCUGGUGUCCGAGCUG 17 5103 HSV2-UL30-1051 - GCUGGUGUCCGAGCUGG 17 3921

HSV2-UL30-2309 - UGGUGUCCGAGCUGGCG 17 5104

HSV2-UL30-2310 - CGAGCUGGCGGAGGAUC 17 5105

HSV2-UL30-2311 - CCCGGGUACGCCAUCGC 17 5106

HSV2-UL30-1055 - CCGGGUACGCCAUCGCC 17 3925

HSV2-UL30-2313 - GGGGCGUUCCGCUCAAC 17 5107

HSV2-UL30-2314 - AUUACUUCUCGCACCUG 17 5108

HSV2-UL30-1059 - UUACUUCUCGCACCUGC 17 3929

HSV2-UL30-1060 - UACUUCUCGCACCUGCU 17 3930

HSV2-UL30-2317 - G ACG U U CAAG GCCCUGU 17 5109

HSV2-UL30-1065 - ACGUUCAAGGCCCUGUU 17 3935

HSV2-UL30-2319 - AAAUAACGCCAAGAUCA 17 5110

HSV2-UL30-2320 - AUAACGCCAAGAUCACC 17 5111

HSV2-UL30-2321 - UCACCGAGAGUCUGUUA 17 5112

HSV2-UL30-2322 - GUUAAAGAGGUUUAUUC 17 5113

HSV2-UL30-2323 - CCGAGACGUGGCACCCC 17 5114

HSV2-UL30-2324 - GACGUGGCCGCGCGGCU 17 5115

HSV2-UL30-2325 - CCGCGCGGCUCAGGGCC 17 5116

HSV2-UL30-1073 - CGCGCGGCUCAGGGCCG 17 3943

HSV2-UL30-2327 - GCUCAGGGCCGCGGGGU 17 5117

HSV2-UL30-2328 - CCGCGGGGUUCGGGCCG 17 5118

HSV2-UL30-1079 - CGCGGGGUUCGGGCCGG 17 3949

HSV2-UL30-1080 - GCGGGGUUCGGGCCGGC 17 3950

HSV2-UL30-2331 - CGGGGGCCGGCGCUACG 17 5119

HSV2-UL30-1085 - GGGGGCCGGCGCUACGG 17 3955

HSV2-UL30-2333 - GGGCCGGCGCUACGGCG 17 5120

HSV2-UL30-1086 - GGCCGGCGCUACGGCGG 17 3956

HSV2-UL30-2335 - ACGGCGGAGGAAACUCG 17 5121

HSV2-UL30-2336 - ACUCGUCGAAUGUUGCA 17 5122

HSV2-UL30-2337 + AAGGCUCUAUGCAACAUUCG 20 5123

HSV2-UL30-2338 + UAGCGCCGGCCCCCGCCGGC 20 5124

HSV2-UL30-2339 + CCGGCCCGAACCCCGCGGCC 20 5125

HSV2-UL30-2340 + GAGCCGCGCGGCCACGUCGU 20 5126

HSV2-UL30-1091 + AGCCGCGCGGCCACGUCGUC 20 3961

HSV2-UL30-1092 + GCCGCGCGGCCACGUCGUCC 20 3962

HSV2-UL30-1093 + CCGCGCGGCCACGUCGUCCG 20 3963

HSV2-UL30-2344 + CGUCCGGGGGGUGCCACGUC 20 5127

HSV2-UL30-1096 + GUCCGGGGGGUGCCACGUCU 20 3966

HSV2-UL30-1097 + UCCGGGGGGUGCCACGUCUC 20 3967

HSV2-UL30-2347 + AUCUUGGCGUUAUUUCCAAA 20 5128

HSV2-UL30-2348 + CGUUAUUUCCAAACAGGGCC 20 5129

HSV2-UL30-2349 + GCAGGCCGCCCCCAGCAGGU 20 5130

HSV2-UL30-2350 + AGGCCGCCCCCAGCAGGUGC 20 5131

HSV2-UL30-2351 + GCGAGAAGUAAUAGUCCGUG 20 5132

HSV2-UL30-2352 + GAAGUAAUAGUCCGUGUUGA 20 5133

HSV2-UL30-1104 + AAGUAAUAGUCCGUGUUGAG 20 3974 HSV2-UL30-2354 + UCCGUGUUGAGCGGAACGCC 20 5134

HSV2-UL30-2355 + CGCCCCGGGCGAUGGCGUAC 20 5135

HSV2-UL30-1108 + GCCCCGGGCGAUGGCGUACC 20 3978

HSV2-UL30-2357 + ACCCGGGAUCCUCCGCCAGC 20 5136

HSV2-UL30-2358 + AGCUCGGACACCAGCAGCUU 20 5137

HSV2-UL30-1111 + GCUCGGACACCAGCAGCUUG 20 3981

HSV2-UL30-2360 + ACACCAGCAGCUUGCGGGGC 20 5138

HSV2-UL30-2361 + GCGGGGCUUGGACGCGCCUC 20 5139

HSV2-UL30-1115 + CGGGGCUUGGACGCGCCUCC 20 3985

HSV2-UL30-1116 + GGGGCUUGGACGCGCCUCCC 20 3986

HSV2-UL30-1117 + GGGCUUGGACGCGCCUCCCG 20 3987

HSV2-UL30-2365 + UCGGCAUGCGACGGCGUCUC 20 5140

HSV2-UL30-1122 + CGGCAUGCGACGGCGUCUCC 20 3992

HSV2-UL30-1123 + GGCAUGCGACGGCGUCUCCC 20 3993

HSV2-UL30-2368 + CGUCUCCCGGGGGCGCUUGG 20 5141

HSV2-UL30-1127 + GUCUCCCGGGGGCGCUUGGC 20 3997

HSV2-UL30-1128 + UCUCCCGGGGGCGCUUGGCC 20 3998

HSV2-UL30-1129 + CUCCCGGGGGCGCU UGGCCG 20 3999

HSV2-UL30-2372 + CCCGGGGGCGCUUGGCCGGG 20 5142

HSV2-UL30-2373 + GGGCGCUUGGCCGGGGAGGG 20 5143

HSV2-UL30-2374 + GGCCGGGGAGGGCAGGGCCG 20 5144

HSV2-UL30-1134 + GCCGGGGAGGGCAGGGCCGC 20 4004

HSV2-UL30-1135 + CCGGGGAGGGCAGGGCCGCU 20 4005

HSV2-UL30-1136 + CGGGGAGGGCAGGGCCGCUG 20 4006

HSV2-UL30-1137 + GGGGAGGGCAGGGCCGCUGG 20 4007

HSV2-UL30-2379 + AGGGCAGGGCCGCUGGGGGG 20 5145

HSV2-UL30-2380 + UGGGGGGGCGGGCUCGUCCC 20 5146

HSV2-UL30-1142 + GGGGGGGCGGGCUCGUCCCC 20 4012

HSV2-UL30-2382 + GGGGCGGCGGCGUCUAGCUC 20 5147

HSV2-UL30-1147 + GGGCGGCGGCGUCUAGCUCG 20 4017

HSV2-UL30-2384 + GCGGCGGCGUCUAGCUCGCG 20 5148

HSV2-UL30-2385 + GCGACCGUCUCCUCUACCUC 20 5149

HSV2-UL30-2386 + GUCUCCUCUACCUCGCGGGU 20 5150

HSV2-UL30-2387 + GGUCUGGGCCACGAUCACGU 20 5151

HSV2-UL30-1155 + GUCUGGGCCACGAUCACGUA 20 4025

HSV2-UL30-2389 + CGUACGGGAUCCGGUCCUUG 20 5152

HSV2-UL30-2390 + CGGGAUCCGGUCCUUGAUGG 20 5153

HSV2-UL30-1159 + GGGAUCCGGUCCUUGAUGGA 20 4029

HSV2-UL30-2392 + AUGGACGGGACCUGCGCGCG 20 5154

HSV2-UL30-2393 + GGACCUGCGCGCGGCGGGCC 20 5155

HSV2-UL30-2394 + AGCUUGUAAUACACCGUCAG 20 5156

HSV2-UL30-2395 + GCGCUUGUUGGUGUACGCGC 20 5157

HSV2-UL30-2396 + GGUGUCUGCUCAGUUCGGCG 20 5158

HSV2-UL30-2397 + UGUCUGCUCAGUUCGGCGGU 20 5159

HSV2-UL30-2398 + UCGGCGGUGAGGACAAAGUC 20 5160

HSV2-UL30-2399 + AAAGUCCUGGAUGUCCCUCU 20 5161 HSV2-UL30-2400 + UCCGGGUCGGUGAUGCGCCG 20 5162

HSV2-UL30-2401 + UGAUGCGCCGAUGGGCGUCU 20 5163

HSV2-UL30-2402 + AUGCGCCGAUGGGCGUCUAC 20 5164

HSV2-UL30-2403 + GGGCGUCUACGAGGACGGCC 20 5165

HSV2-UL30-2404 + CCCCGAACGCCUGCAGUCCC 20 5166

HSV2-UL30-2405 + AACGCCUGCAGUCCCUCGGG 20 5167

HSV2-UL30-1184 + ACGCCUGCAGUCCCUCGGGC 20 4054

HSV2-UL30-2407 + GUCGCGCCAGCCACUCCUCU 20 5168

HSV2-UL30-1187 + UCGCGCCAGCCACUCCUCUG 20 4057

HSV2-UL30-2409 + CGGCUAACGCGGCGGCCGCU 20 5169

HSV2-UL30-2410 + U CG U AAAACAG CAG G U CG AC 20 5170

HSV2-UL30-2411 + ACAGCAGGUCGACCAGGGCC 20 5171

HSV2-UL30-1198 + CAGCAGGUCGACCAGGGCCC 20 4068

HSV2-UL30-2413 + GCACCAGAUCCACGCCCUUG 20 5172

HSV2-UL30-2414 + CGAUGUACUUUUUCUUGGCG 20 5173

HSV2-UL30-2415 + UGGCGAUGAGCAGCAGCUUG 20 5174

HSV2-UL30-2416 + UGGUGAACGUUUUUUCGCAC 20 5175

HSV2-UL30-2417 + UUUUUCGCACUCGAGCUUGA 20 5176

HSV2-UL30-1203 + UUUUCGCACUCGAGCUUGAU 20 4073

HSV2-UL30-1204 + UUUCGCACUCGAGCUUGAUC 20 4074

HSV2-UL30-1205 + UUCGCACUCGAGCUUGAUCG 20 4075

HSV2-UL30-1206 + UCGCACUCGAGCUUGAUCGG 20 4076

HSV2-UL30-1207 + CGCACUCGAGCUUGAUCGGG 20 4077

HSV2-UL30-2423 + CACUCGAGCUUGAUCGGGGG 20 5177

HSV2-UL30-1208 + ACUCGAGCUUGAUCGGGGGG 20 4078

HSV2-UL30-2425 + CGGGGGGAGGAACAGCGCGC 20 5178

HSV2-UL30-2426 + UGGCCACCAGGCCCGCGGCC 20 5179

HSV2-UL30-2427 + CCGUGAGGCCGCGGCACAAA 20 5180

HSV2-UL30-2428 + GGCCGCGGCACAAAACGAAA 20 5181

HSV2-UL30-1215 + G CCG CG G CACAAAACG AAAA 20 4085

HSV2-UL30-2430 + UGUCCCCGUAGAUGAUGCGC 20 5182

HSV2-UL30-1216 + GUCCCCGUAGAUGAUGCGCA 20 4086

HSV2-UL30-2432 + GUAGAUGAUGCGCAUGGAGU 20 5183

HSV2-UL30-2433 + UGAUGCGCAUGGAGUACGGA 20 5184

HSV2-UL30-1218 + GAUGCGCAUGGAGUACGGAC 20 4088

HSV2-UL30-1219 + AUGCGCAUGGAGUACGGACC 20 4089

HSV2-UL30-2436 + GGCGCGCAUGCCGGCCGCCU 20 5185

HSV2-UL30-1223 + GCGCGCAUGCCGGCCGCCUC 20 4093

HSV2-UL30-2438 + GAAAGUCGGCCAGCAGCUGA 20 5186

HSV2-UL30-2439 + CGUGCACGUACGCGCGCGUC 20 5187

HSV2-UL30-2440 + UGCACGUACGCGCGCGUCGC 20 5188

HSV2-UL30-1225 + GCACGUACGCGCGCGUCGCG 20 4095

HSV2-UL30-2442 + CGGUGGCGGCCACGUGCAGG 20 5189

HSV2-UL30-2443 + GCGGCCACGUGCAGGCAGGG 20 5190

HSV2-UL30-2444 + GAAGACCGUGCUGCACCCCG 20 5191

HSV2-UL30-2445 + CACCCCGG UGAACCCG U ACA 20 5192 HSV2-UL30-2446 + UGAUGGCGGCCUGUUGCUUG 20 5193

HSV2-UL30-2447 + AUGGCGGCCUGUUGCUUGUC 20 5194

HSV2-UL30-1237 + UGGCGGCCUGUUGCUUGUCG 20 4107

HSV2-UL30-2449 + GCGGCCUGUUGCUUGUCGAG 20 5195

HSV2-UL30-2450 + UGUCGAGGAGGACGGCCUCC 20 5196

HSV2-UL30-1240 + GUCGAGGAGGACGGCCUCCU 20 4110

HSV2-UL30-1241 + UCGAGGAGGACGGCCUCCUC 20 4111

HSV2-UL30-2453 + ACGGCCUCCUCGGGGGUGCU 20 5197

HSV2-UL30-1244 + CGGCCUCCUCGGGGGUGCUC 20 4114

HSV2-UL30-1245 + GGCCUCCUCGGGGGUGCUCU 20 4115

HSV2-UL30-1246 + GCCUCCUCGGGGGUGCUCUG 20 4116

HSV2-UL30-2457 + GGGGGUGCUCUGGGGGAUCC 20 5198

HSV2-UL30-2458 + GUGCUCUGGGGGAUCCGCGA 20 5199

HSV2-UL30-2459 + AUGGCCAGCCAGUCGCGCAG 20 5200

HSV2-UL30-2460 + CUCAGCAGGCUCUCGCGUAC 20 5201

HSV2-UL30-2461 + UCUCGCGUACGUGGGCCUUC 20 5202

HSV2-UL30-2462 + CGCGUACGUGGGCCUUCACG 20 5203

HSV2-UL30-2463 + CCUCCAGGUGCGCGACGGCC 20 5204

HSV2-UL30-2464 + UGCGCGACGGCCUCGGGCCG 20 5205

HSV2-UL30-1261 + GCGCGACGGCCUCGGGCCGC 20 4131

HSV2-UL30-1262 + CGCGACGGCCUCGGGCCGCA 20 4132

HSV2-UL30-2467 + CGACGGCCUCGGGCCGCAGG 20 5206

HSV2-UL30-2468 + CGGGCCGCAGGGAGAGCGUA 20 5207

HSV2-UL30-2469 + AGCGUACUGAAGCACAGGUU 20 5208

HSV2-UL30-2470 + CUGAAGCACAGGUUGUGGGC 20 5209

HSV2-UL30-2471 + GGUUGUGGGCCUGGAUGAUG 20 5210

HSV2-UL30-1267 + GUUGUGGGCCUGGAUGAUGC 20 4137

HSV2-UL30-2473 + U G G CAAAG U CAAACACCACC 20 5211

HSV2-UL30-1272 + G G CAAAG U CAAACACC ACCA 20 4142

HSV2-UL30-2475 + AACACCACCACGGGGUCGAC 20 5212

HSV2-UL30-2476 + CCACGGGGUCGACGUG AAAC 20 5213

HSV2-UL30-1275 + CACGGGGUCGACGUG AAACC 20 4145

HSV2-UL30-2478 + GGUCGACGUGAAACCCGGAG 20 5214

HSV2-UL30-1277 + GUCGACGUGAAACCCGGAGG 20 4147

HSV2-UL30-2480 + CGUGAAACCCGGAGGUGGGG 20 5215

HSV2-UL30-2481 + UGAAACCCGGAGGUGGGGUC 20 5216

HSV2-UL30-2482 + CCGGAGGUGGGGUCGAGGAC 20 5217

HSV2-UL30-2483 + CCCGGCCGCUCCCCUUCCCC 20 5218

HSV2-UL30-2484 + CCCUUCCCCCCGAGGCACGG 20 5219

HSV2-UL30-2485 + CCCGAGGCACGGCCGGGCGC 20 5220

HSV2-UL30-2486 + GGCGCU UGGGCGCCUCCUUG 20 5221

HSV2-UL30-2487 + GGCGCCUCCUUGUCGAGGCC 20 5222

HSV2-UL30-2488 + UCGAGGCCCCGAAACCGCCC 20 5223

HSV2-UL30-2489 + AACCGCCCCUGGGUGUCCGG 20 5224

HSV2-UL30-2490 + GCCCCUGGGUGUCCGGCAGG 20 5225

HSV2-UL30-2491 + CCCUUCUGGCCCGCAAGGCG 20 5226 HSV2-UL30-1300 + CCUUCUGGCCCGCAAGGCGC 20 4170

HSV2-UL30-2493 + CCG C AAG G CG CAG G AG G CAC 20 5227

HSV2-UL30-2494 + CGCAGGAGGCACGUGAAGAC 20 5228

HSV2-UL30-2495 + UGCUGGCCGUCGUAGAUGGU 20 5229

HSV2-UL30-2496 + UGCCCGCCAGGCGCGCGACG 20 5230

HSV2-UL30-1309 + GCCCGCCAGGCGCGCGACGG 20 4179

HSV2-UL30-2498 + GCGGAAAGCUCCAGGUGCGG 20 5231

HSV2-UL30-2499 + GCUCCAGGUGCGGCAGAAAC 20 5232

HSV2-UL30-2500 + CCAGGUGCGGCAGAAACUUG 20 5233

HSV2-UL30-2501 + GAACAGCUGCCCGACCAGCA 20 5234

HSV2-UL30-2502 + CGCCGAUCACCCCGCGCUGC 20 5235

HSV2-UL30-2503 + UCACCCCGCGCUGCGCGGGC 20 5236

HSV2-UL30-1314 + CACCCCGCGCUGCGCGGGCC 20 4184

HSV2-UL30-2505 + CGGGCCCGGAGGCGUAGUAG 20 5237

HSV2-UL30-1317 + GGGCCCGGAGGCGUAGUAGG 20 4187

HSV2-UL30-1318 + GGCCCGGAGGCGUAGUAGGC 20 4188

HSV2-UL30-2508 + CGACGGCGUUCAGCUUGUAG 20 5238

HSV2-UL30-1324 + GACGGCGUUCAGCUUGUAGC 20 4194

HSV2-UL30-2510 + CGGCGUUCAGCUUGUAGCUG 20 5239

HSV2-UL30-2511 + ACCUUGAUCUUGCUGCGCUU 20 5240

HSV2-UL30-2512 + CUCUGGCCGAUGUCCCACAC 20 5241

HSV2-UL30-1328 + UCUGGCCGAUGUCCCACACG 20 4198

HSV2-UL30-2514 + CGU UCAUGCGCCCGUACCCG 20 5242

HSV2-UL30-2515 + CAUGCGCCCGUACCCGUCGA 20 5243

HSV2-UL30-1330 + AUGCGCCCGUACCCGUCGAG 20 4200

HSV2-UL30-2517 + UAGAUCUCCGUCAGCUUGGU 20 5244

HSV2-UL30-2518 + UCUCCGUCAGCUUGGUCAGG 20 5245

HSV2-UL30-2519 + CCGUCAGCUUGGUCAGGACG 20 5246

HSV2-UL30-2520 + UGGUCAGGACGAAGGGCCAG 20 5247

HSV2-UL30-2521 + UGAUGAUGUUGUACCCGGUC 20 5248

HSV2-UL30-2522 + UGUUGUACCCGGUCACGAAC 20 5249

HSV2-UL30-1337 + GUUGUACCCGGUCACGAACU 20 4207

HSV2-UL30-2524 + ACUCGGGGCCGUACUGCUUG 20 5250

HSV2-UL30-2525 + CGUACUGCUUGACGAAGGUC 20 5251

HSV2-UL30-2526 + UCAUGAAGGCCAGCAGCAUC 20 5252

HSV2-UL30-2527 + U CG AAU U CGCU G U CAAACU C 20 5253

HSV2-UL30-2528 + CGCUGUCAAACUCCAGGACG 20 5254

HSV2-UL30-1343 + GCUGUCAAACUCCAGGACGA 20 4213

HSV2-UL30-1344 + CU G U CAAACU CCAGGACG AC 20 4214

HSV2-UL30-2531 + CGACGGGGGCCGGCAGGCCC 20 5255

HSV2-UL30-1349 + GACGGGGGCCGGCAGGCCCC 20 4219

HSV2-UL30-2533 + GGGCCGGCAGGCCCCUGGAG 20 5256

HSV2-UL30-2534 + GGCCCCUGGAGGCGAGAUCG 20 5257

HSV2-UL30-2535 + CUGGAGGCGAGAUCGCUGAG 20 5258

HSV2-UL30-1352 + UGGAGGCGAGAUCGCUGAGG 20 4222

HSV2-UL30-2537 + CGAGAUCGCUGAGGUGGGAC 20 5259 HSV2-UL30-1354 + GAGAUCGCUGAGGUGGGACU 20 4224

HSV2-UL30-1355 + AGAUCGCUGAGGUGGGACUC 20 4225

HSV2-UL30-1356 + GAUCGCUGAGGUGGGACUCG 20 4226

HSV2-UL30-2541 + GGUGGGACUCGGGGAGGUCG 20 5260

HSV2-UL30-2542 + ACUCGGGGAGGUCGCAGGAU 20 5261

HSV2-UL30-2543 + GGGGAGGUCGCAGGAUCCGA 20 5262

HSV2-UL30-2544 + UCGCAGGAUCCGAGCGAAAA 20 5263

HSV2-UL30-1359 + CGCAGGAUCCGAGCGAAAAC 20 4229

HSV2-UL30-2546 + CAGG AU CCG AG CG AAAACAG 20 5264

HSV2-UL30-2547 + GCGAAAACAGGAGGAUGUGC 20 5265

HSV2-UL30-2548 + GAAAACAGGAGGAUGUGCUC 20 5266

HSV2-UL30-2549 + GGAUGUGCUCGAGGGCGGUG 20 5267

HSV2-UL30-2550 + GGGCGGUGGUGGACAGGUCG 20 5268

HSV2-UL30-2551 + UGGACAGGUCGUAGAGCAGA 20 5269

HSV2-UL30-1367 + GGACAGGUCGUAGAGCAGAC 20 4237

HSV2-UL30-2553 + UCGUAGAGCAGACAGGAGAU 20 5270

HSV2-UL30-2554 + GCAGACAGGAGAUCUGGAUG 20 5271

HSV2-UL30-2555 + GAUCUGGAUGACGAGGUCUU 20 5272

HSV2-UL30-2556 + GUCUUCCGGGCGUUCCGCGA 20 5273

HSV2-UL30-1372 + UCUUCCGGGCGUUCCGCGAC 20 4242

HSV2-UL30-2558 + CCCCGGCCUUGCAUUCGAUA 20 5274

HSV2-UL30-2559 + UGCAUUCGAUAUCGAAGCAC 20 5275

HSV2-UL30-2560 + GCAGUUAAACUCGACGUCGC 20 5276

HSV2-UL30-2561 + ACUCGACGUCGCUCGAGGUU 20 5277

HSV2-UL30-2562 + GCUCGAGGUUCCGAACGCCG 20 5278

HSV2-UL30-1382 + CUCGAGGUUCCGAACGCCGU 20 4252

HSV2-UL30-1383 + UCGAGGUUCCGAACGCCGUC 20 4253

HSV2-UL30-1384 + CGAGGUUCCGAACGCCGUCG 20 4254

HSV2-UL30-2566 + AACGCCGUCGGGGGGCGCGG 20 5279

HSV2-UL30-2567 + GGGCCGGCGCGU UCCCGCGG 20 5280

HSV2-UL30-2568 + GCGCGUUCCCGCGGCCGGGC 20 5281

HSV2-UL30-2569 + CGGGCUUGAGGCGGUACCAG 20 5282

HSV2-UL30-2570 + CCAG CCG AAG G U G ACAAACC 20 5283

HSV2-UL30-2571 + GUGACAAACCCCGGGUUGUC 20 5284

HSV2-UL30-2572 + CCCGGGUUGUCCAGGAUAAA 20 5285

HSV2-UL30-2573 + CCCCCUCGUACUUCCUGAUC 20 5286

HSV2-UL30-1404 + CCCCUCGUACUUCCUGAUCG 20 4274

HSV2-UL30-2575 + CCAGCGCGCGCCCGCUUCGC 20 5287

HSV2-UL30-2576 + CGCACGAAGACGCGGUAGUA 20 5288

HSV2-UL30-2577 + GAAGACGCGGUAGUACAGGG 20 5289

HSV2-UL30-2578 + CGCG CU CCACCACCU CCG CC 20 5290

HSV2-UL30-2579 + CCUCCGCCUCGAAGUGGUCC 20 5291

HSV2-UL30-1416 + CUCCGCCUCGAAGUGGUCCG 20 4286

HSV2-UL30-2581 + AAGUGGUCCGCGGAGAUGCC 20 5292

HSV2-UL30-1417 + AGUGGUCCGCGGAGAUGCCG 20 4287

HSV2-UL30-2583 + GACGCCCCCGGCGACUCGCG 20 5293 HSV2-UL30-2584 + GGGCCGCCGCCAGGCGCUCG 20 5294

HSV2-UL30-2585 + CAGGCGCUCGCAGAGAUCGC 20 5295

HSV2-UL30-1422 + AGGCGCUCGCAGAGAUCGCG 20 4292

HSV2-UL30-2587 + UACUGCCGCGUGCCGUAGAC 20 5296

HSV2-UL30-2588 + GAACGGCGACGCGAUGGCCU 20 5297

HSV2-UL30-1429 + AACGGCGACGCGAUGGCCUU 20 4299

HSV2-UL30-1430 + ACGGCGACGCGAUGGCCUUC 20 4300

HSV2-UL30-2591 + UGGCCUUCGGGGGUCAGACC 20 5298

HSV2-UL30-2592 + GAAGCGUGAUGACGGUCCCG 20 5299

HSV2-UL30-2593 + AUGGCGUCCAUAAAUCGCUC 20 5300

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-2595 + GCUCUAUGCAACAUUCG 17 5301

HSV2-UL30-2596 + CGCCGGCCCCCGCCGGC 17 5302

HSV2-UL30-2597 + GCCCGAACCCCGCGGCC 17 5303

HSV2-UL30-2598 + CCGCGCGGCCACGUCGU 17 5304

HSV2-UL30-1443 + CGCGCGGCCACGUCGUC 17 4313

HSV2-UL30-1444 + GCGCGGCCACGUCGUCC 17 4314

HSV2-UL30-1445 + CGCGGCCACGUCGUCCG 17 4315

HSV2-UL30-2602 + CCGGGGGGUGCCACGUC 17 5305

HSV2-UL30-1448 + CGGGGGGUGCCACGUCU 17 4318

HSV2-UL30-1449 + GGGGGGUGCCACGUCUC 17 4319

HSV2-UL30-2605 + UUGGCGUUAUUUCCAAA 17 5306

HSV2-UL30-2606 + UAUUUCCAAACAGGGCC 17 5307

HSV2-UL30-2607 + GGCCGCCCCCAGCAGGU 17 5308

HSV2-UL30-2608 + CCGCCCCCAGCAGGUGC 17 5309

HSV2-UL30-2609 + AGAAGUAAUAGUCCGUG 17 5310

HSV2-UL30-2610 + GUAAUAGUCCGUGUUGA 17 5311

HSV2-UL30-1456 + UAAUAGUCCGUGUUGAG 17 4326

HSV2-UL30-2612 + GUGUUGAGCGGAACGCC 17 5312

HSV2-UL30-2613 + CCCGGGCGAUGGCGUAC 17 5313

HSV2-UL30-1460 + CCGGGCGAUGGCGUACC 17 4330

HSV2-UL30-2615 + CGGGAUCCUCCGCCAGC 17 5314

HSV2-UL30-2616 + UCGGACACCAGCAGCUU 17 5315

HSV2-UL30-1463 + CG G ACACCAG CAGCU U G 17 4333

HSV2-UL30-2618 + CCAGCAGCUUGCGGGGC 17 5316

HSV2-UL30-2619 + GGGCUUGGACGCGCCUC 17 5317

HSV2-UL30-1467 + GGCUUGGACGCGCCUCC 17 4337

HSV2-UL30-1468 + GCUUGGACGCGCCUCCC 17 4338

HSV2-UL30-1469 + CUUGGACGCGCCUCCCG 17 4339

HSV2-UL30-2623 + GCAUGCGACGGCGUCUC 17 5318

HSV2-UL30-1474 + CAUGCGACGGCGUCUCC 17 4344

HSV2-UL30-1475 + AUGCGACGGCGUCUCCC 17 4345

HSV2-UL30-2626 + CUCCCGGGGGCGCUUGG 17 5319

HSV2-UL30-1479 + UCCCGGGGGCGCUUGGC 17 4349

HSV2-UL30-1480 + CCCGGGGGCGCUUGGCC 17 4350

HSV2-UL30-1481 + CCGGGGGCGCUUGGCCG 17 4351 HSV2-UL30-2630 + GGGGGCGCUUGGCCGGG 17 5320

HSV2-UL30-2631 + CGCUUGGCCGGGGAGGG 17 5321

HSV2-UL30-2632 + CGGGGAGGGCAGGGCCG 17 5322

HSV2-UL30-1486 + GGGGAGGGCAGGGCCGC 17 4356

HSV2-UL30-1487 + GGGAGGGCAGGGCCGCU 17 4357

HSV2-UL30-1488 + GGAGGGCAGGGCCGCUG 17 4358

HSV2-UL30-1489 + GAGGGCAGGGCCGCUGG 17 4359

HSV2-UL30-2637 + GCAGGGCCGCUGGGGGG 17 5323

HSV2-UL30-2638 + GGGGGCGGGCUCGUCCC 17 5324

HSV2-UL30-1494 + GGGGCGGGCUCGUCCCC 17 4364

HSV2-UL30-2640 + GCGGCGGCGUCUAGCUC 17 5325

HSV2-UL30-1499 + CGGCGGCGUCUAGCUCG 17 4369

HSV2-UL30-2642 + GCGGCGUCUAGCUCGCG 17 5326

HSV2-UL30-2643 + ACCGUCUCCUCUACCUC 17 5327

HSV2-UL30-2644 + UCCUCUACCUCGCGGGU 17 5328

HSV2-UL30-2645 + CUGGGCCACGAUCACGU 17 5329

HSV2-UL30-1507 + UGGGCCACGAUCACGUA 17 4377

HSV2-UL30-2647 + ACGGGAUCCGGUCCUUG 17 5330

HSV2-UL30-2648 + GAUCCGGUCCUUGAUGG 17 5331

HSV2-UL30-1511 + AUCCGGUCCUUGAUGGA 17 4381

HSV2-UL30-2650 + GACGGGACCUGCGCGCG 17 5332

HSV2-UL30-2651 + CCUGCGCGCGGCGGGCC 17 5333

HSV2-UL30-2652 + UUGUAAUACACCGUCAG 17 5334

HSV2-UL30-2653 + CUUGUUGGUGUACGCGC 17 5335

HSV2-UL30-2654 + GUCUGCUCAGUUCGGCG 17 5336

HSV2-UL30-2655 + CUGCUCAGUUCGGCGGU 17 5337

HSV2-UL30-2656 + G CG G U G AG G ACAAAG U C 17 5338

HSV2-UL30-2657 + GUCCUGGAUGUCCCUCU 17 5339

HSV2-UL30-2658 + GGGUCGGUGAUGCGCCG 17 5340

HSV2-UL30-2659 + UGCGCCGAUGGGCGUCU 17 5341

HSV2-UL30-2660 + CGCCGAUGGGCGUCUAC 17 5342

HSV2-UL30-2661 + CGUCUACGAGGACGGCC 17 5343

HSV2-UL30-2662 + CGAACGCCUGCAGUCCC 17 5344

HSV2-UL30-2663 + GCCUGCAGUCCCUCGGG 17 5345

HSV2-UL30-1536 + CCUGCAGUCCCUCGGGC 17 4406

HSV2-UL30-2665 + GCGCCAGCCACUCCUCU 17 5346

HSV2-UL30-1539 + CGCCAG CCACU CCU CU G 17 4409

HSV2-UL30-2667 + CUAACGCGGCGGCCGCU 17 5347

HSV2-UL30-2668 + UAAAACAGCAGGUCGAC 17 5348

HSV2-UL30-2669 + GCAGGUCGACCAGGGCC 17 5349

HSV2-UL30-1550 + CAGGUCGACCAGGGCCC 17 4420

HSV2-UL30-2671 + CCAGAUCCACGCCCU UG 17 5350

HSV2-UL30-2672 + UGUACUUUUUCUUGGCG 17 5351

HSV2-UL30-2673 + CGAUGAGCAGCAGCUUG 17 5352

HSV2-UL30-2674 + UGAACGUUUUUUCGCAC 17 5353

HSV2-UL30-2675 + UUCGCACUCGAGCUUGA 17 5354 HSV2-UL30-1555 + UCGCACUCGAGCUUGAU 17 4425

HSV2-UL30-1556 + CGCACUCGAGCUUGAUC 17 4426

HSV2-UL30-1557 + GCACUCGAGCUUGAUCG 17 4427

HSV2-UL30-1558 + CACUCGAGCUUGAUCGG 17 4428

HSV2-UL30-1559 + ACUCGAGCUUGAUCGGG 17 4429

HSV2-UL30-2681 + UCGAGCUUGAUCGGGGG 17 5355

HSV2-UL30-1560 + CGAGCUUGAUCGGGGGG 17 4430

HSV2-UL30-2683 + GGGGAGGAACAGCGCGC 17 5356

HSV2-UL30-2684 + CCACCAGGCCCGCGGCC 17 5357

HSV2-UL30-2685 + UGAGGCCGCGGCACAAA 17 5358

HSV2-UL30-2686 + CGCGGCACAAAACGAAA 17 5359

HSV2-UL30-1567 + GCGG CACAAAACG AAAA 17 4437

HSV2-UL30-2688 + CCCCGUAGAUGAUGCGC 17 5360

HSV2-UL30-1568 + CCCGUAGAUGAUGCGCA 17 4438

HSV2-UL30-2690 + GAUGAUGCGCAUGGAGU 17 5361

HSV2-UL30-2691 + UGCGCAUGGAGUACGGA 17 5362

HSV2-UL30-1570 + GCGCAUGGAGUACGGAC 17 4440

HSV2-UL30-1571 + CGCAUGGAGUACGGACC 17 4441

HSV2-UL30-2694 + GCGCAUGCCGGCCGCCU 17 5363

HSV2-UL30-1575 + CGCAUGCCGGCCGCCUC 17 4445

HSV2-UL30-2696 + AGUCGGCCAGCAGCUGA 17 5364

HSV2-UL30-2697 + GCACGUACGCGCGCGUC 17 5365

HSV2-UL30-2698 + ACGUACGCGCGCGUCGC 17 5366

HSV2-UL30-1577 + CGUACGCGCGCGUCGCG 17 4447

HSV2-UL30-2700 + UGGCGGCCACGUGCAGG 17 5367

HSV2-UL30-2701 + GCCACGUGCAGGCAGGG 17 5368

HSV2-UL30-2702 + GACCG UGCUG CACCCCG 17 5369

HSV2-UL30-2703 + CCCGGUGAACCCGUACA 17 5370

HSV2-UL30-2704 + UGGCGGCCUGUUGCUUG 17 5371

HSV2-UL30-2705 + GCGGCCUGUUGCUUGUC 17 5372

HSV2-UL30-1589 + CGGCCUGUUGCUUGUCG 17 4459

HSV2-UL30-2707 + GCCUGUUGCUUGUCGAG 17 5373

HSV2-UL30-2708 + CGAGGAGGACGGCCUCC 17 5374

HSV2-UL30-1592 + GAGGAGGACGGCCUCCU 17 4462

HSV2-UL30-1593 + AGGAGGACGGCCUCCUC 17 4463

HSV2-UL30-2711 + GCCUCCUCGGGGGUGCU 17 5375

HSV2-UL30-1596 + CCUCCUCGGGGGUGCUC 17 4466

HSV2-UL30-1597 + CUCCUCGGGGGUGCUCU 17 4467

HSV2-UL30-1598 + UCCUCGGGGGUGCUCUG 17 4468

HSV2-UL30-2715 + GGUGCUCUGGGGGAUCC 17 5376

HSV2-UL30-2716 + CUCUGGGGGAUCCGCGA 17 5377

HSV2-UL30-2717 + GCCAGCCAGUCGCGCAG 17 5378

HSV2-UL30-2718 + AGCAGGCUCUCGCGUAC 17 5379

HSV2-UL30-2719 + CGCGUACGUGGGCCUUC 17 5380

HSV2-UL30-2720 + GUACGUGGGCCUUCACG 17 5381

HSV2-UL30-2721 + CCAGGUGCGCGACGGCC 17 5382 HSV2-UL30-2722 + GCGACGGCCUCGGGCCG 17 5383

HSV2-UL30-1613 + CGACGGCCUCGGGCCGC 17 4483

HSV2-UL30-1614 + GACGGCCUCGGGCCGCA 17 4484

HSV2-UL30-2725 + CGGCCUCGGGCCGCAGG 17 5384

HSV2-UL30-2726 + GCCGCAGGGAGAGCGUA 17 5385

HSV2-UL30-2727 + GUACUGAAGCACAGGUU 17 5386

HSV2-UL30-2728 + AAGCACAGGUUGUGGGC 17 5387

HSV2-UL30-2729 + UGUGGGCCUGGAUGAUG 17 5388

HSV2-UL30-1619 + GUGGGCCUGGAUGAUGC 17 4489

HSV2-UL30-2731 + CA AAG U CAAACACCACC 17 5389

HSV2-UL30-1624 + AAAG U CAAACACCACCA 17 4494

HSV2-UL30-2733 + ACCACCACGGGGUCGAC 17 5390

HSV2-UL30-2734 + CGGGGUCGACGUGAAAC 17 5391

HSV2-UL30-1627 + GGGGUCGACGUGAAACC 17 4497

HSV2-UL30-2736 + CGACGUGAAACCCGGAG 17 5392

HSV2-UL30-1629 + GACGUGAAACCCGGAGG 17 4499

HSV2-UL30-2738 + GAAACCCGGAGGUGGGG 17 5393

HSV2-UL30-2739 + AACCCGGAGGUGGGGUC 17 5394

HSV2-UL30-2740 + GAGGUGGGGUCGAGGAC 17 5395

HSV2-UL30-2741 + GGCCGCUCCCCUUCCCC 17 5396

HSV2-UL30-2742 + UUCCCCCCGAGGCACGG 17 5397

HSV2-UL30-2743 + GAGGCACGGCCGGGCGC 17 5398

HSV2-UL30-2744 + GCU UGGGCGCCUCCUUG 17 5399

HSV2-UL30-2745 + GCCUCCUUGUCGAGGCC 17 5400

HSV2-UL30-2746 + AGGCCCCGAAACCGCCC 17 5401

HSV2-UL30-2747 + CGCCCCUGGGUGUCCGG 17 5402

HSV2-UL30-2748 + CCUGGGUGUCCGGCAGG 17 5403

HSV2-UL30-2749 + UUCUGGCCCGCAAGGCG 17 5404

HSV2-UL30-1652 + UCUGGCCCGCAAGGCGC 17 4522

HSV2-UL30-2751 + CAAGGCGCAGGAGGCAC 17 5405

HSV2-UL30-2752 + AGGAGGCACGUG AAG AC 17 5406

HSV2-UL30-2753 + UGGCCGUCGUAGAUGGU 17 5407

HSV2-UL30-2754 + CCGCCAGGCGCGCGACG 17 5408

HSV2-UL30-1661 + CGCCAGGCGCGCGACGG 17 4531

HSV2-UL30-2756 + GAAAGCUCCAGGUGCGG 17 5409

HSV2-UL30-2757 + CCAGGUGCGG CAG AAAC 17 5410

HSV2-UL30-2758 + GGUGCGGCAGAAACUUG 17 5411

HSV2-UL30-2759 + CAGCUGCCCGACCAGCA 17 5412

HSV2-UL30-2760 + CGAUCACCCCGCGCUGC 17 5413

HSV2-UL30-2761 + CCCCGCGCUGCGCGGGC 17 5414

HSV2-UL30-1666 + CCCGCGCUGCGCGGGCC 17 4536

HSV2-UL30-2763 + GCCCGGAGGCGUAGUAG 17 5415

HSV2-UL30-1669 + CCCGGAGGCGUAGUAGG 17 4539

HSV2-UL30-1670 + CCGGAGGCGUAGUAGGC 17 4540

HSV2-UL30-2766 + CGGCGUUCAGCUUGUAG 17 5416

HSV2-UL30-1676 + GGCGUUCAGCUUGUAGC 17 4546 HSV2-UL30-2768 + CGUUCAGCUUGUAGCUG 17 5417

HSV2-UL30-2769 + UUGAUCUUGCUGCGCUU 17 5418

HSV2-UL30-2770 + UGGCCGAUGUCCCACAC 17 5419

HSV2-UL30-1680 + GGCCGAUGUCCCACACG 17 4550

HSV2-UL30-2772 + UCAUGCGCCCGUACCCG 17 5420

HSV2-UL30-2773 + GCGCCCGUACCCGUCGA 17 5421

HSV2-UL30-1682 + CGCCCGUACCCGUCGAG 17 4552

HSV2-UL30-2775 + AUCUCCGUCAGCUUGGU 17 5422

HSV2-UL30-2776 + CCGUCAGCUUGGUCAGG 17 5423

HSV2-UL30-2777 + UCAGCUUGGUCAGGACG 17 5424

HSV2-UL30-2778 + UCAGGACGAAGGGCCAG 17 5425

HSV2-UL30-2779 + UGAUGUUGUACCCGGUC 17 5426

HSV2-UL30-2780 + UGUACCCGGUCACGAAC 17 5427

HSV2-UL30-1689 + GUACCCGGUCACGAACU 17 4559

HSV2-UL30-2782 + CGGGGCCGUACUGCUUG 17 5428

HSV2-UL30-2783 + ACUGCUUGACGAAGGUC 17 5429

HSV2-UL30-2784 + UGAAGGCCAGCAGCAUC 17 5430

HSV2-UL30-2785 + AAUUCGCUGUCAAACUC 17 5431

HSV2-UL30-2786 + UGUCAAACUCCAGGACG 17 5432

HSV2-UL30-1695 + GUCAAACUCCAGGACGA 17 4565

HSV2-UL30-1696 + UCAAACUCCAGGACGAC 17 4566

HSV2-UL30-2789 + CGGGGGCCGGCAGGCCC 17 5433

HSV2-UL30-1701 + GGGGGCCGGCAGGCCCC 17 4571

HSV2-UL30-2791 + CCGGCAGGCCCCUGGAG 17 5434

HSV2-UL30-2792 + CCCUGGAGGCGAGAUCG 17 5435

HSV2-UL30-2793 + GAGGCGAGAUCGCUGAG 17 5436

HSV2-UL30-1704 + AGGCGAGAUCGCUGAGG 17 4574

HSV2-UL30-2795 + GAUCGCUGAGGUGGGAC 17 5437

HSV2-UL30-1706 + AUCGCUGAGGUGGGACU 17 4576

HSV2-UL30-1707 + UCGCUGAGGUGGGACUC 17 4577

HSV2-UL30-1708 + CGCUGAGGUGGGACUCG 17 4578

HSV2-UL30-2799 + GGGACUCGGGGAGGUCG 17 5438

HSV2-UL30-2800 + CGGGGAGGUCGCAGGAU 17 5439

HSV2-UL30-2801 + GAGGUCGCAGGAUCCGA 17 5440

HSV2-UL30-2802 + CAGGAUCCGAGCGAAAA 17 5441

HSV2-UL30-1711 + AGGAUCCGAGCGAAAAC 17 4581

HSV2-UL30-2804 + GAUCCGAGCGAAAACAG 17 5442

HSV2-UL30-2805 + AAAACAGGAGGAUGUGC 17 5443

HSV2-UL30-2806 + AACAGGAGGAUGUGCUC 17 5444

HSV2-UL30-2807 + UGUGCUCGAGGGCGGUG 17 5445

HSV2-UL30-2808 + CGGUGGUGGACAGGUCG 17 5446

HSV2-UL30-2809 + ACAG G U CG U AG AG CAG A 17 5447

HSV2-UL30-1719 + CAGGUCGUAGAGCAGAC 17 4589

HSV2-UL30-2811 + UAGAGCAGACAGGAGAU 17 5448

HSV2-UL30-2812 + GACAGGAGAUCUGGAUG 17 5449

HSV2-UL30-2813 + CUGGAUGACGAGGUCUU 17 5450 HSV2-UL30-2814 + UUCCGGGCGUUCCGCGA 17 5451

HSV2-UL30-1724 + UCCGGGCGUUCCGCGAC 17 4594

HSV2-UL30-2816 + CGGCCUUGCAUUCGAUA 17 5452

HSV2-UL30-2817 + AUUCGAUAUCGAAGCAC 17 5453

HSV2-UL30-2818 + GUUAAACUCGACGUCGC 17 5454

HSV2-UL30-2819 + CGACGUCGCUCGAGGUU 17 5455

HSV2-UL30-2820 + CGAGGUUCCGAACGCCG 17 5456

HSV2-UL30-1734 + GAGGUUCCGAACGCCGU 17 4604

HSV2-UL30-1735 + AGGUUCCGAACGCCGUC 17 4605

HSV2-UL30-1736 + GGUUCCGAACGCCGUCG 17 4606

HSV2-UL30-2824 + GCCGUCGGGGGGCGCGG 17 5457

HSV2-UL30-2825 + CCGGCGCGUUCCCGCGG 17 5458

HSV2-UL30-2826 + CGUUCCCGCGGCCGGGC 17 5459

HSV2-UL30-2827 + GCUUGAGGCGGUACCAG 17 5460

HSV2-UL30-2828 + GCCGAAGGUGACAAACC 17 5461

HSV2-UL30-2829 + ACAAACCCCGGGUUGUC 17 5462

HSV2-UL30-2830 + GGGUUGUCCAGGAUAAA 17 5463

HSV2-UL30-2831 + CCUCGUACUUCCUGAUC 17 5464

HSV2-UL30-1756 + CUCGUACUUCCUGAUCG 17 4626

HSV2-UL30-2833 + GCGCGCGCCCGCUUCGC 17 5465

HSV2-UL30-2834 + ACGAAGACGCGGUAGUA 17 5466

HSV2-UL30-2835 + GACGCGGUAGUACAGGG 17 5467

HSV2-UL30-2836 + G CU CCACCACCU CCGCC 17 5468

HSV2-UL30-2837 + CCGCCUCGAAGUGGUCC 17 5469

HSV2-UL30-1768 + CGCCUCGAAGUGGUCCG 17 4638

HSV2-UL30-2839 + UGGUCCGCGGAGAUGCC 17 5470

HSV2-UL30-1769 + GGUCCGCGGAGAUGCCG 17 4639

HSV2-UL30-2841 + GCCCCCGGCGACUCGCG 17 5471

HSV2-UL30-2842 + CCGCCGCCAGGCGCUCG 17 5472

HSV2-UL30-2843 + GCGCUCGCAGAGAUCGC 17 5473

HSV2-UL30-1774 + CGCUCGCAGAGAUCGCG 17 4644

HSV2-UL30-2845 + UGCCGCGUGCCGUAGAC 17 5474

HSV2-UL30-2846 + CGGCGACGCGAUGGCCU 17 5475

HSV2-UL30-1781 + GGCGACGCGAUGGCCUU 17 4651

HSV2-UL30-1782 + GCGACGCGAUGGCCUUC 17 4652

HSV2-UL30-2849 + CCUUCGGGGGUCAGACC 17 5476

HSV2-UL30-2850 + GCGUGAUGACGGUCCCG 17 5477

HSV2-UL30-2851 + GCGUCCAUAAAUCGCUC 17 5478

HSV2-UL30-1790 + CGUCCAUAAAUCGCUCG 17 4660

Table 2F provides exemplary targeting domains for knocking out the UL30 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL30 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 2F

Table 3A provides exemplary targeting domains for knocking out the UL48 gene selected according to first tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL48 gene and orthogonality against the human genome. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3A

HSV2-UL48-3 GAUCUGUUUGCGGACGCGGA 20 5484

HSV2-UL48-116 CGAUCUGUUUGCGGACG 17 5485

HSV2-UL48-117 CUGUUUGCGGACGCGGA 17 5486

HSV2-UL48-5 UCUGUUUGCGGACGCGGACG 20 5487

HSV2-UL48-119 GUUUGCGGACGCGGACG 17 5488

HSV2-UL48-11 GCCCCUCCGCUGUACGCCAC 20 5489

HSV2-UL48-12 CUCCGCUGUACGCCACCGGU 20 5490

HSV2-UL48-13 CGCCACCGGUCGGCUGAGUC 20 5491

HSV2-UL48-125 CCUCCGCUGUACGCCAC 17 5492

HSV2-UL48-126 CGCUGUACGCCACCGGU 17 5493

HSV2-UL48-17 CGACGAUCUGGGCUUCAGCG 20 5494

HSV2-UL48-18 GACGAUCUGGGCUUCAGCGC 20 5495

HSV2-UL48-131 CGAUCUGGGCUUCAGCG 17 5496

HSV2-UL48-132 GAUCUGGGCUUCAGCGC 17 5497

HSV2-UL48-20 CAUGCUAGAUACCUGGAACG 20 5498

HSV2-UL48-134 GCUAGAUACCUGGAACG 17 5499

HSV2-UL48-24 GACCAACGCCGACAUGUACC 20 5500

HSV2-UL48-25 UGCCCAGCGACGUGAUCGAC 20 5501

HSV2-UL48-138 CAACGCCGACAUGUACC 17 5502

HSV2-UL48-139 CCAGCGACGUGAUCGAC 17 5503

HSV2-UL48-29 CAGCGACGUGAUCGACUGGG 20 5504

HSV2-UL48-30 CCGAUCGACAUUCGCGCCCA 20 5505

HSV2-UL48-31 CAUUCGCGCCCACGGCGACG 20 5506

HSV2-UL48-32 CGAGCUGCCUUCGUACUACG 20 5507

HSV2-UL48-33 GCCUUCGUACUACGAGGCCA 20 5508

HSV2-UL48-34 GCCAUGGCGCAGUUUUUCCG 20 5509

HSV2-UL48-35 AGUUUUUCCGCGGUGAGCUG 20 5510

HSV2-UL48-36 GUUUUUCCGCGGUGAGCUGC 20 5511

HSV2-UL48-143 CGACGUGAUCGACUGGG 17 5512

HSV2-UL48-144 AUCGACAUUCGCGCCCA 17 5513

HSV2-UL48-145 UCGCGCCCACGGCGACG 17 5514

HSV2-UL48-146 GCUGCCUUCGUACUACG 17 5515

HSV2-UL48-147 UUCGUACUACGAGGCCA 17 5516

HSV2-UL48-148 AUGGCGCAGUUUUUCCG 17 5517

HSV2-UL48-149 UUUUCCGCGGUGAGCUG 17 5518

HSV2-UL48-150 UUUCCGCGGUGAGCUGC 17 5519

HSV2-UL48-42 + ACCGCGGAAAAACUGCGCCA 20 5520

HSV2-UL48-43 + GCCAUGGCCUCGUAGUACGA 20 5521

HSV2-UL48-44 + AGUACGAAGGCAGCUCGUCG 20 5522

HSV2-UL48-45 + GUACGAAGGCAGCUCGUCGC 20 5523

HSV2-UL48-46 + CGAAGGCAGCUCGUCGCGGG 20 5524

HSV2-UL48-47 + AGGCAGCUCGUCGCGGGUGG 20 5525

HSV2-UL48-48 + GGCAGCUCGUCGCGGGUGGC 20 5526

HSV2-UL48-156 + GCGGAAAAACUGCGCCA 17 5527

HSV2-UL48-157 + AUGGCCUCGUAGUACGA 17 5528

HSV2-UL48-158 + ACGAAGGCAGCUCGUCG 17 5529 HSV2-UL48-159 + CGAAGGCAGCUCGUCGC 17 5530

HSV2-UL48-160 + AGGCAGCUCGUCGCGGG 17 5531

HSV2-UL48-161 + CAGCUCGUCGCGGGUGG 17 5532

HSV2-UL48-162 + AGCUCGUCGCGGGUGGC 17 5533

HSV2-UL48-50 + CUCGUCGCGGGUGGCGGGCA 20 5534

HSV2-UL48-164 + GUCGCGGGUGGCGGGCA 17 5535

HSV2-UL48-55 + GGGGAACGCCACGUCGCCGU 20 5536

HSV2-UL48-56 + CCGUGGGCGCGAAUGUCGAU 20 5537

HSV2-UL48-57 + CGUGGGCGCGAAUGUCGAUC 20 5538

HSV2-UL48-58 + GUGGGCGCGAAUGUCGAUCG 20 5539

HSV2-UL48-59 + AAUGUCGAUCGGGGAGCGCU 20 5540

HSV2-UL48-60 + AUGUCGAUCGGGGAGCGCUC 20 5541

HSV2-UL48-61 + UGUCGAUCGGGGAGCGCUCG 20 5542

HSV2-UL48-62 + CCCCCAGUCGAUCACGUCGC 20 5543

HSV2-UL48-63 + CCCCAGUCGAUCACGUCGCU 20 5544

HSV2-UL48-169 + GAACGCCACGUCGCCGU 17 5545

HSV2-UL48-170 + UGGGCGCGAAUGUCGAU 17 5546

HSV2-UL48-171 + GGGCGCGAAUGUCGAUC 17 5547

HSV2-UL48-172 + GGCGCGAAUGUCGAUCG 17 5548

HSV2-UL48-173 + GUCGAUCGGGGAGCGCU 17 5549

HSV2-UL48-174 + UCGAUCGGGGAGCGCUC 17 5550

HSV2-UL48-175 + CGAUCGGGGAGCGCUCG 17 5551

HSV2-UL48-176 + CCAGUCGAUCACGUCGC 17 5552

HSV2-UL48-177 + CAGUCGAUCACGUCGCU 17 5553

HSV2-UL48-65 + CUUGCACUCCCGGUACAUGU 20 5554

HSV2-UL48-66 + CUCCCGGUACAUGUCGGCGU 20 5555

HSV2-UL48-67 + CGGUACAUGUCGGCGUUGGU 20 5556

HSV2-UL48-68 + GGUACAUGUCGGCGUUGGUC 20 5557

HSV2-UL48-179 + GCACUCCCGGUACAUGU 17 5558

HSV2-UL48-180 + CCGGUACAUGUCGGCGU 17 5559

HSV2-UL48-181 + UACAUGUCGGCGUUGGU 17 5560

HSV2-UL48-182 + ACAUGUCGGCGUUGGUC 17 5561

HSV2-UL48-71 + CUCGUUCCAGGUAUCUAGCA 20 5562

HSV2-UL48-185 + GUUCCAGGUAUCUAGCA 17 5563

HSV2-UL48-73 + UCUAGCAUGGUACACAGCGC 20 5564

HSV2-UL48-187 + AGCAUGGUACACAGCGC 17 5565

HSV2-UL48-76 + CGUCGAGGAGACGGUUAAAC 20 5566

HSV2-UL48-77 + GUCGAGGAGACGGUUAAACA 20 5567

HSV2-UL48-78 + GAGACGGUUAAACAGGGCCG 20 5568

HSV2-UL48-79 + AGACGGUUAAACAGGGCCGC 20 5569

HSV2-UL48-80 + GACGGUUAAACAGGGCCGCG 20 5570

HSV2-UL48-81 + ACGGUUAAACAGGGCCGCGG 20 5571

HSV2-UL48-82 + CGGUUAAACAGGGCCGCGGG 20 5572

HSV2-UL48-190 + CGAGGAGACGGUUAAAC 17 5573

HSV2-UL48-191 + GAGGAGACGGUUAAACA 17 5574

HSV2-UL48-192 + ACGGUUAAACAGGGCCG 17 5575 HSV2-UL48-193 + CGGUUAAACAGGGCCGC 17 5576

HSV2-UL48-194 + GGUUAAACAGGGCCGCG 17 5577

HSV2-UL48-195 + GUUAAACAGGGCCGCGG 17 5578

HSV2-UL48-196 + UUAAACAGGGCCGCGGG 17 5579

HSV2-UL48-94 + GGCCUGACUCAGCCGACCGG 20 5580

HSV2-UL48-95 + AGCCGACCGGUGGCGUACAG 20 5581

HSV2-UL48-97 + GACCGGUGGCGUACAGCGGA 20 5582

HSV2-UL48-98 + ACCGGUGGCGUACAGCGGAG 20 5583

HSV2-UL48-208 + CUGACUCAGCCGACCGG 17 5584

HSV2-UL48-209 + CGACCGGUGGCGUACAG 17 5585

HSV2-UL48-211 + CGGUGGCGUACAGCGGA 17 5586

HSV2-UL48-212 + GGUGGCGUACAGCGGAG 17 5587

HSV2-UL48-101 + CGUACAGCGGAGGGGCGGCU 20 5588

HSV2-UL48-215 + ACAGCGGAGGGGCGGCU 17 5589

HSV2-UL48-106 + GUGUUCUUGGGACCCCCGGC 20 5590

HSV2-UL48-114 + CAAACAGAUCGUCGACCAAC 20 5591

HSV2-UL48-220 + UUCUUGGGACCCCCGGC 17 5592

HSV2-UL48-228 + ACAGAUCGUCGACCAAC 17 5593

Table 3B provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL48 gene. It is

contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3B

HSV2-UL48-8 CACCGCCCCCCAGGCCAGCC 20 5598

HSV2-UL48-9 ACCGCCCCCCAGGCCAGCCG 20 5599

HSV2-UL48-10 CCGCCCCCCAGGCCAGCCGG 20 5600

HSV2-UL48-14 GCCCAUGCCCGUCCCCCCCG 20 5601

HSV2-UL48-15 UAACCGUCUCCUCGACGAUC 20 5602

HSV2-UL48-16 AACCGUCUCCUCGACGAUCU 20 5603

HSV2-UL48-19 UGUGUACCAUGCUAGAUACC 20 5604

HSV2-UL48-21 UGGAACGAGGACCUGUUCUC 20 5605

HSV2-UL48-22 GGAACGAGGACCUGUUCUCU 20 5606

HSV2-UL48-23 CGACCAACGCCGACAUGUAC 20 5607

HSV2-UL48-26 GCCCAGCGACGUGAUCGACU 20 5608

HSV2-UL48-27 CCCAGCGACGUGAUCGACUG 20 5609

HSV2-UL48-28 CCAGCGACGUGAUCGACUGG 20 5610

HSV2-UL48-37 UCCGCGGUGAGCUGCGGGCG 20 5611

HSV2-UL48-38 CCGCGGUGAGCUGCGGGCGC 20 5612

HSV2-UL48-39 CGGUGAGCUGCGGGCGCGGG 20 5613

HSV2-UL48-40 GGGCGCGGGAGGAGAGCUAC 20 5614

HSV2-UL48-41 + CCCGCGCCCGCAGCUCACCG 20 5615

HSV2-UL48-49 + GCUCGUCGCGGGUGGCGGGC 20 5616

HSV2-UL48-51 + GUCGCGGGUGGCGGGCAGGG 20 5617

HSV2-UL48-52 + UCGCGGGUGGCGGGCAGGGU 20 5618

HSV2-UL48-53 + CGCGGGUGGCGGGCAGGGUG 20 5619

HSV2-UL48-54 + UGGGGAACGCCACGUCGCCG 20 5620

HSV2-UL48-64 + UCGACAGAAACUUGCACUCC 20 5621

HSV2-UL48-69 + UGGUCGGGAACCCAGAGAAC 20 5622

HSV2-UL48-70 + AGAGAACAGGUCCUCGUUCC 20 5623

HSV2-UL48-72 + AUCUAGCAUGGUACACAGCG 20 5624

HSV2-UL48-74 + CGCUGAAGCCCAGAUCGUCG 20 5625

HSV2-UL48-75 + AGCCCAGAUCGUCGAGGAGA 20 5626

HSV2-UL48-83 + GGUUAAACAGGGCCGCGGGG 20 5627

HSV2-UL48-84 + AAACAGGGCCGCGGGGGGGA 20 5628

HSV2-UL48-85 + AACAGGGCCGCGGGGGGGAC 20 5629

HSV2-UL48-86 + GGCCGCGGGGGGGACGGGCA 20 5630

HSV2-UL48-87 + GCCGCGGGGGGGACGGGCAU 20 5631

HSV2-UL48-88 + GCGGGGGGGACGGGCAUGGG 20 5632

HSV2-UL48-89 + GGGGACGGGCAUGGGCGGCG 20 5633

HSV2-UL48-90 + GGGACGGGCAUGGGCGGCGA 20 5634

HSV2-UL48-91 + UGGGCGGCGAGGGCAUCAGC 20 5635

HSV2-UL48-92 + GGGCGGCGAGGGCAUCAGCU 20 5636

HSV2-UL48-93 + CUGGGCCUGACUCAGCCGAC 20 5637

HSV2-UL48-96 + CGACCGGUGGCGUACAGCGG 20 5638

HSV2-UL48-99 + GGUGGCGUACAGCGGAGGGG 20 5639

HSV2-UL48-100 + GCGUACAGCGGAGGGGCGGC 20 5640

HSV2-UL48-102 + GUACAGCGGAGGGGCGGCUG 20 5641

HSV2-UL48-103 + AGGGGCGGCUGGGGUGUUCU 20 5642

HSV2-UL48-104 + GGGGCGGCUGGGGUGUUCUU 20 5643 HSV2-UL48-105 + UGGGGUGUUCUUGGGACCCC 20 5644

HSV2-UL48-107 + CUUGGGACCCCCGGCUGGCC 20 5645

HSV2-UL48-108 + UUGGGACCCCCGGCUGGCCU 20 5646

HSV2-UL48-109 + UGGGACCCCCGGCUGGCCUG 20 5647

HSV2-UL48-110 + GGGACCCCCGGCUGGCCUGG 20 5648

HSV2-UL48-111 + GGACCCCCGGCUGGCCUGGG 20 5649

HSV2-UL48-112 + CCCCCGGCUGGCCUGGGGGG 20 5650

HSV2-UL48-113 + CCGGCUGGCCUGGGGGGCGG 20 5651

HSV2-UL48-115 GGUCGACGAUCUGUUUG 17 5652

HSV2-UL48-118 UGUUUGCGGACGCGGAC 17 5653

HSV2-UL48-120 UUUCGCCACCGCCCCCC 17 5654

HSV2-UL48-121 CCGCCCCCCAGGCCAGC 17 5655

HSV2-UL48-122 CGCCCCCCAGGCCAGCC 17 5656

HSV2-UL48-123 GCCCCCCAGGCCAGCCG 17 5657

HSV2-UL48-124 CCCCCCAGGCCAGCCGG 17 5658

HSV2-UL48-128 CAUGCCCGUCCCCCCCG 17 5659

HSV2-UL48-129 CCGUCUCCUCGACGAUC 17 5660

HSV2-UL48-130 CGUCUCCUCGACGAUCU 17 5661

HSV2-UL48-133 GUACCAUGCUAGAUACC 17 5662

HSV2-UL48-135 AACGAGGACCUGUUCUC 17 5663

HSV2-UL48-136 ACGAGGACCUGUUCUCU 17 5664

HSV2-UL48-137 CCAACGCCGACAUGUAC 17 5665

HSV2-UL48-140 CAGCGACGUGAUCGACU 17 5666

HSV2-UL48-141 AGCGACGUGAUCGACUG 17 5667

HSV2-UL48-142 GCGACGUGAUCGACUGG 17 5668

HSV2-UL48-151 GCGGUGAGCUGCGGGCG 17 5669

HSV2-UL48-152 CGGUGAGCUGCGGGCGC 17 5670

HSV2-UL48-153 UGAGCUGCGGGCGCGGG 17 5671

HSV2-UL48-154 CGCGGGAGGAGAGCUAC 17 5672

HSV2-UL48-155 + GCGCCCGCAGCUCACCG 17 5673

HSV2-UL48-163 + CGUCGCGGGUGGCGGGC 17 5674

HSV2-UL48-165 + GCGGGUGGCGGGCAGGG 17 5675

HSV2-UL48-166 + CGGGUGGCGGGCAGGGU 17 5676

HSV2-UL48-167 + GGGUGGCGGGCAGGGUG 17 5677

HSV2-UL48-168 + GGAACGCCACGUCGCCG 17 5678

HSV2-UL48-178 + ACAGAAACUUGCACUCC 17 5679

HSV2-UL48-183 + UCGGGAACCCAGAGAAC 17 5680

HSV2-UL48-184 + GAACAGGUCCUCGUUCC 17 5681

HSV2-UL48-186 + UAGCAUGGUACACAGCG 17 5682

HSV2-UL48-188 + UGAAGCCCAGAUCGUCG 17 5683

HSV2-UL48-189 + CCAGAUCGUCGAGGAGA 17 5684

HSV2-UL48-197 + UAAACAGGGCCGCGGGG 17 5685

HSV2-UL48-198 + CAGGGCCGCGGGGGGGA 17 5686

HSV2-UL48-199 + AGGGCCGCGGGGGGGAC 17 5687

HSV2-UL48-200 + CGCGGGGGGGACGGGCA 17 5688

HSV2-UL48-201 + GCGGGGGGGACGGGCAU 17 5689 HSV2-UL48-202 + GGGGGGACGGGCAUGGG 17 5690

HSV2-UL48-203 + GACGGGCAUGGGCGGCG 17 5691

HSV2-UL48-204 + ACGGGCAUGGGCGGCGA 17 5692

HSV2-UL48-205 + GCGGCGAGGGCAUCAGC 17 5693

HSV2-UL48-206 + CGGCGAGGGCAUCAGCU 17 5694

HSV2-UL48-207 + GGCCUGACUCAGCCGAC 17 5695

HSV2-UL48-210 + CCGGUGGCGUACAGCGG 17 5696

HSV2-UL48-213 + GGCGUACAGCGGAGGGG 17 5697

HSV2-UL48-214 + UACAGCGGAGGGGCGGC 17 5698

HSV2-UL48-216 + CAGCGGAGGGGCGGCUG 17 5699

HSV2-UL48-217 + GGCGGCUGGGGUGUUCU 17 5700

HSV2-UL48-218 + GCGGCUGGGGUGUUCUU 17 5701

HSV2-UL48-219 + GGUGUUCUUGGGACCCC 17 5702

HSV2-UL48-221 + GGGACCCCCGGCUGGCC 17 5703

HSV2-UL48-222 + GGACCCCCGGCUGGCCU 17 5704

HSV2-UL48-223 + GACCCCCGGCUGGCCUG 17 5705

HSV2-UL48-224 + ACCCCCGGCUGGCCUGG 17 5706

HSV2-UL48-225 + CCCCCGGCUGGCCUGGG 17 5707

HSV2-UL48-226 + CCGGCUGGCCUGGGGGG 17 5708

HSV2-UL48-227 + GCUGGCCUGGGGGGCGG 17 5709

HSV2-UL48-175 CGAUCGGGGAGCGCUCG 17 5551

Table 3C provides exemplary targeting domains for knocking out the UL48 gene selected according to the third tier parameters. The targeting domains are selected based on location within the coding sequence, but downstream of the first 500bp of the UL48 gene. In an embodiment, the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands and that are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3C

HSV2-UL48-231 CGUUCGGCAGCUACACCGCC 20 5713

HSV2-UL48-232 UACACCGCCAGGCACACAUG 20 5714

HSV2-UL48-233 ACACCGCCAGGCACACAUGC 20 5715

HSV2-UL48-234 CACCGCCAGGCACACAUGCG 20 5716

HSV2-UL48-235 GGGGCCGCAACCGCGACCUG 20 5717

HSV2-UL48-236 GGGCCGCAACCGCGACCUGC 20 5718

HSV2-UL48-237 GAUGCUGCGCACCACGAUCG 20 5719

HSV2-UL48-238 UGCGCACCACGAUCGCGGAC 20 5720

HSV2-UL48-239 CUACCGCGAGACCGCGCGCC 20 5721

HSV2-UL48-240 UUCUGAGCCGCGAGAUCCUA 20 5722

HSV2-UL48-241 UCUGAGCCGCGAGAUCCUAU 20 5723

HSV2-UL48-242 CGUACGCCGAGCAGAUGAUG 20 5724

HSV2-UL48-243 AUGCGGCCCGAUCUGUUCGA 20 5725

HSV2-UL48-244 CGACGGCCUCUGCUGCGACC 20 5726

HSV2-UL48-245 UCUGCUGCGACCUGGAGAGC 20 5727

HSV2-UL48-246 CCUGGAGAGCUGGCGCCAGU 20 5728

HSV2-UL48-247 CAGCCCCUGAUGUUUAUCAA 20 5729

HSV2-UL48-248 UCAACGGAUCGCUCACCGUG 20 5730

HSV2-UL48-249 CAACGGAUCGCUCACCGUGC 20 5731

HSV2-UL48-250 AACGGAUCGCUCACCGUGCG 20 5732

HSV2-UL48-251 CACCGUGCGGGGAGUUCCCG 20 5733

HSV2-UL48-252 CGUGCGGGGAGUUCCCGUGG 20 5734

HSV2-UL48-253 GGGGAGUUCCCGUGGAGGCC 20 5735

HSV2-UL48-254 CCGUGGAGGCCCGGCGACUG 20 5736

HSV2-UL48-255 CGUGGAGGCCCGGCGACUGC 20 5737

HSV2-UL48-256 CGAGCACCUGAACCUCCCGC 20 5738

HSV2-UL48-257 CCUCCCGCUGGUGCGAAGUG 20 5739

HSV2-UL48-258 CCCGCUGGUGCGAAGUGCGG 20 5740

HSV2-UL48-259 GCUGGUGCGAAGUGCGGCGG 20 5741

HSV2-UL48-260 GGUGCGAAGUGCGGCGGCGG 20 5742

HSV2-UL48-261 AGUGCGGCGGCGGAGGAACC 20 5743

HSV2-UL48-262 GUGCGGCGGCGGAGGAACCC 20 5744

HSV2-UL48-263 UGCGGCGGCGGAGGAACCCG 20 5745

HSV2-UL48-264 CACGACCCCGCCCGUCCUGC 20 5746

HSV2-UL48-265 ACGACCCCGCCCGUCCUGCA 20 5747

HSV2-UL48-266 GCCCGUCCUGCAGGGCAACC 20 5748

HSV2-UL48-267 GGCAACCAGGCCCGCUCCUC 20 5749

HSV2-UL48-268 GCAACCAGGCCCGCUCCUCU 20 5750

HSV2-UL48-269 GGUACUUUAUGCUGCUGAUC 20 5751

HSV2-UL48-270 GUACUUUAUGCUGCUGAUCC 20 5752

HSV2-UL48-271 GCUGCUGAUCCGGGCCAAGU 20 5753

HSV2-UL48-272 GUACUCCAGCGUCGCGACCU 20 5754

HSV2-UL48-273 CUCCAGCGUCGCGACCUCGG 20 5755

HSV2-UL48-274 UCCAGCGUCGCGACCUCGGA 20 5756

HSV2-UL48-275 CGGAGGGCGAGUCCGUCAUG 20 5757

HSV2-UL48-276 GGAGGGCGAGUCCGUCAUGC 20 5758 HSV2-UL48-277 CGGGAGCACGCGUAUAGCCG 20 5759

HSV2-UL48-278 GGGAGCACGCGUAUAGCCGC 20 5760

HSV2-UL48-279 AGCACGCGUAUAGCCGCGGG 20 5761

HSV2-UL48-280 GGGCGGACCAGAAACAAUUA 20 5762

HSV2-UL48-281 CAAUUACGGAUCGACAAUCG 20 5763

HSV2-UL48-282 AAUUACGGAUCGACAAUCGA 20 5764

HSV2-UL48-283 CGAGGGCCUGCUCGACCUCC 20 5765

HSV2-UL48-284 GGACGACGAUGACGCUCCUG 20 5766

HSV2-UL48-285 CGACGAUGACGCUCCUGCGG 20 5767

HSV2-UL48-286 GAUGACGCUCCUGCGGAGGC 20 5768

HSV2-UL48-287 AUGACGCUCCUGCGGAGGCC 20 5769

HSV2-UL48-288 CGCUCCUGCGGAGGCCGGGC 20 5770

HSV2-UL48-289 UCCUGCGGAGGCCGGGCUGG 20 5771

HSV2-UL48-290 GCGCAUGUCGUUUCUCUCCG 20 5772

HSV2-UL48-291 CGCAUGUCGUUUCUCUCCGC 20 5773

HSV2-UL48-292 CCCCAUUACCGACGUCAGCC 20 5774

HSV2-UL48-293 CCCAUUACCGACGUCAGCCU 20 5775

HSV2-UL48-294 CCUGGGAGACGAACUCCGCC 20 5776

HSV2-UL48-295 GGAGACGAACUCCGCCUGGA 20 5777

HSV2-UL48-296 CGAACUCCGCCUGGACGGCG 20 5778

HSV2-UL48-297 ACUCCGCCUGGACGGCGAGG 20 5779

HSV2-UL48-298 CCGCCUGGACGGCGAGGAGG 20 5780

HSV2-UL48-299 UAUGACGCCCGCCGACGCCC 20 5781

HSV2-UL48-300 CGCCCUGGACGACUUCGACU 20 5782

HSV2-UL48-301 CGACUUCGACUUGGAGAUGC 20 5783

HSV2-UL48-302 GACUUCGACUUGGAGAUGCU 20 5784

HSV2-UL48-303 ACUUCGACUUGGAGAUGCUG 20 5785

HSV2-UL48-304 CUUCGACUUGGAGAUGCUGG 20 5786

HSV2-UL48-305 CUUGGAGAUGCUGGGGGACG 20 5787

HSV2-UL48-306 GGACGUGGAGUCCCCCUCCC 20 5788

HSV2-UL48-307 GACGUGGAGUCCCCCUCCCC 20 5789

HSV2-UL48-308 ACCCACGACCCCGUCUCGUA 20 5790

HSV2-UL48-309 CCCACGACCCCGUCUCGUAU 20 5791

HSV2-UL48-310 CCACGACCCCGUCUCGUAUG 20 5792

HSV2-UL48-311 CCCCGUCUCGUAUGGGGCUU 20 5793

HSV2-UL48-312 CUCGUAUGGGGCUUUGGACG 20 5794

HSV2-UL48-313 ACAGAUGUUUACCGAUGCCA 20 5795

HSV2-UL48-314 CAGAUGUUUACCGAUGCCAU 20 5796

HSV2-UL48-315 GCCAUGGGCAUUGACGACUU 20 5797

HSV2-UL48-316 CCAUGGGCAUUGACGACUUU 20 5798

HSV2-UL48-317 CAUGGGCAUUGACGACUUUG 20 5799

HSV2-UL48-318 AUGGGCAUUGACGACUUUGG 20 5800

HSV2-UL48-319 UGGGCAUUGACGACUUUGGG 20 5801

HSV2-UL48-320 + CCCAAAGUCGUCAAUGCCCA 20 5802

HSV2-UL48-321 + GUCGUCAAUGCCCAUGGCAU 20 5803

HSV2-UL48-322 + GUCCAAAGCCCCAUACGAGA 20 5804 HSV2-UL48-323 + UCCAAAGCCCCAUACGAGAC 20 5805

HSV2-UL48-324 + CCAAAGCCCCAUACGAGACG 20 5806

HSV2-UL48-325 + CCCCAUACGAGACGGGGUCG 20 5807

HSV2-UL48-326 + CCCAUACGAGACGGGGUCGU 20 5808

HSV2-UL48-327 + ACGGGGUCGUGGGUCAUUCC 20 5809

HSV2-UL48-328 + CGGGGUCGUGGGUCAUUCCC 20 5810

HSV2-UL48-329 + GGGGUCGUGGGUCAUUCCCG 20 5811

HSV2-UL48-330 + GUCGUGGGUCAUUCCCGGGG 20 5812

HSV2-UL48-331 + UCGUGGGUCAUUCCCGGGGA 20 5813

HSV2-UL48-332 + CGUGGGUCAUUCCCGGGGAG 20 5814

HSV2-UL48-333 + GUGGGUCAUUCCCGGGGAGG 20 5815

HSV2-UL48-334 + UCUCCAAGUCGAAGUCGUCC 20 5816

HSV2-UL48-335 + CUCCAAGUCGAAGUCGUCCA 20 5817

HSV2-UL48-336 + GUCGAAGUCGUCCAGGGCGU 20 5818

HSV2-UL48-337 + GAAGUCGUCCAGGGCGUCGG 20 5819

HSV2-UL48-338 + AAGUCGUCCAGGGCGUCGGC 20 5820

HSV2-UL48-339 + UAUCCACCUCCUCGCCGUCC 20 5821

HSV2-UL48-340 + CCACCUCCUCGCCGUCCAGG 20 5822

HSV2-UL48-341 + CCAGGCGGAGUUCGUCUCCC 20 5823

HSV2-UL48-342 + UUCGUCUCCCAGGCUGACGU 20 5824

HSV2-UL48-343 + UCCCAGGCUGACGUCGGUAA 20 5825

HSV2-UL48-344 + CCCAGGCUGACGUCGGUAAU 20 5826

HSV2-UL48-345 + CCAGGCUGACGUCGGUAAUG 20 5827

HSV2-UL48-346 + CAGGCUGACGUCGGUAAUGG 20 5828

HSV2-UL48-347 + GCUGACGUCGGUAAUGGGGG 20 5829

HSV2-UL48-348 + GACGUCGGUAAUGGGGGCGG 20 5830

HSV2-UL48-349 + GUCGGUAAUGGGGGCGGUGG 20 5831

HSV2-UL48-350 + GGGCGGUGGUGGACAGUCUG 20 5832

HSV2-UL48-351 + GGCGGUGGUGGACAGUCUGC 20 5833

HSV2-UL48-352 + GCGGUGGUGGACAGUCUGCG 20 5834

HSV2-UL48-353 + CGGUGGUGGACAGUCUGCGG 20 5835

HSV2-UL48-354 + UCUGCGGGGGCGUUGUCCCG 20 5836

HSV2-UL48-355 + GCGGAGAGAAACGACAUGCG 20 5837

HSV2-UL48-356 + CAUGCGCGGCGCCACCAGCC 20 5838

HSV2-UL48-357 + GCCACCAGCCCGGCCUCCGC 20 5839

HSV2-UL48-358 + GCAGGAGCGUCAUCGUCGUC 20 5840

HSV2-UL48-359 + CAGGAGCGUCAUCGUCGUCC 20 5841

HSV2-UL48-360 + GAGCGUCAUCGUCGUCCGGG 20 5842

HSV2-UL48-361 + CGUCGUCCGGGAGGUCGAGC 20 5843

HSV2-UL48-362 + UGUCGAUCCGUAAUUGUUUC 20 5844

HSV2-UL48-363 + AAUUGUUUCUGGUCCGCCCG 20 5845

HSV2-UL48-364 + AUACGCGUGCUCCCGCAUGA 20 5846

HSV2-UL48-365 + CAUGACGGACUCGCCCUCCG 20 5847

HSV2-UL48-366 + GCCCUCCGAGGUCGCGACGC 20 5848

HSV2-UL48-367 + GCUGGAGUACGAGUCCAACU 20 5849

HSV2-UL48-368 + AGUACGAGUCCAACUUGGCC 20 5850 HSV2-UL48-369 + CAGCAGCAUAAAGUACCCAG 20 5851

HSV2-UL48-370 + GCAUAAAGUACCCAGAGGAG 20 5852

HSV2-UL48-371 + CAUAAAGUACCCAGAGGAGC 20 5853

HSV2-UL48-372 + AGUACCCAGAGGAGCGGGCC 20 5854

HSV2-UL48-373 + AGCGGGCCUGGUUGCCCUGC 20 5855

HSV2-UL48-374 + GGCCUGGUUGCCCUGCAGGA 20 5856

HSV2-UL48-375 + GCCUGGUUGCCCUGCAGGAC 20 5857

HSV2-UL48-376 + UGGUUGCCCUGCAGGACGGG 20 5858

HSV2-UL48-377 + GGUUGCCCUGCAGGACGGGC 20 5859

HSV2-UL48-378 + GUUGCCCUGCAGGACGGGCG 20 5860

HSV2-UL48-379 + GCAGGACGGGCGGGGUCGUG 20 5861

HSV2-UL48-380 + CAGGACGGGCGGGGUCGUGA 20 5862

HSV2-UL48-381 + AGGACGGGCGGGGUCGUGAG 20 5863

HSV2-UL48-382 + CGGGGUCGUGAGGGGCGCCC 20 5864

HSV2-UL48-383 + GGGGUCGUGAGGGGCGCCCC 20 5865

HSV2-UL48-384 + GCCGCCGCACUUCGCACCAG 20 5866

HSV2-UL48-385 + CCGCCGCACUUCGCACCAGC 20 5867

HSV2-UL48-386 + CCGCACUUCGCACCAGCGGG 20 5868

HSV2-UL48-387 + UUCGCACCAGCGGGAGGUUC 20 5869

HSV2-UL48-388 + GGUUCAGGUGCUCGCGAAUG 20 5870

HSV2-UL48-389 + GGUUUAGCUCCCGCAGUCGC 20 5871

HSV2-UL48-390 + GUUUAGCUCCCGCAGUCGCC 20 5872

HSV2-UL48-391 + CCGCAGUCGCCGGGCCUCCA 20 5873

HSV2-UL48-392 + CGCAGUCGCCGGGCCUCCAC 20 5874

HSV2-UL48-393 + CUCCACGGGAACUCCCCGCA 20 5875

HSV2-UL48-394 + GCGAUCCGUUGAUAAACAUC 20 5876

HSV2-UL48-395 + CGAUCCGUUGAUAAACAUCA 20 5877

HSV2-UL48-396 + GAUCCGUUGAUAAACAUCAG 20 5878

HSV2-UL48-397 + GCUGAAACAGACACGCCAAC 20 5879

HSV2-UL48-398 + CCAACUGGCGCCAGCUCUCC 20 5880

HSV2-UL48-399 + AGCUCUCCAGGUCGCAGCAG 20 5881

HSV2-UL48-400 + GCAGAGGCCGUCGAACAGAU 20 5882

HSV2-UL48-401 + CAGAGGCCGUCGAACAGAUC 20 5883

HSV2-UL48-402 + AUCGGGCCGCAUCAUCUGCU 20 5884

HSV2-UL48-403 + CAUCAUCUGCUCGGCGUACG 20 5885

HSV2-UL48-404 + GCUCGGCGUACGCGGCCCAU 20 5886

HSV2-UL48-405 + ACGCGGCCCAUAGGAUCUCG 20 5887

HSV2-UL48-406 + GGAUCUCGCGGCUCAGAAAG 20 5888

HSV2-UL48-407 + AGAGGUAUAGAUGCAGAAAC 20 5889

HSV2-UL48-408 + GCAGAAACAGGACGCGCGCC 20 5890

HSV2-UL48-409 + CAGGACGCGCGCCAGGCGCG 20 5891

HSV2-UL48-410 + GCGCCAGGCGCGCGGUCUCG 20 5892

HSV2-UL48-411 + GUAGUACCUGUCCGCGAUCG 20 5893

HSV2-UL48-412 + UGGUGCGCAGCAUCUCCCGC 20 5894

HSV2-UL48-413 + GCAGCAUCUCCCGCAGGUCG 20 5895

HSV2-UL48-414 + UCUCCCGCAGGUCGCGGUUG 20 5896 HSV2-UL48-415 + UGCGGCCCCGCAUGUGUGCC 20 5897

HSV2-UL48-416 + GGCCCCGCAUGUGUGCCUGG 20 5898

HSV2-UL48-417 + GCGGUGUAGCUGCCGAACGC 20 5899

HSV2-UL48-418 + GCUGCCGAACGCUGGCGCGC 20 5900

HSV2-UL48-419 + GAACGCUGGCGCGCAGGUAC 20 5901

HSV2-UL48-420 + UGGCGCGCAGGUACCGGUAC 20 5902

HSV2-UL48-421 + GGCGCGCAGGUACCGGUACA 20 5903

HSV2-UL48-422 GUUGGCAAAUUUUUGCU 17 5904

HSV2-UL48-423 UUUGCUCGGCCCUGUAC 17 5905

HSV2-UL48-424 ACCUGCGCGCCAGCGUU 17 5906

HSV2-UL48-425 UCGGCAGCUACACCGCC 17 5907

HSV2-UL48-426 ACCGCCAGGCACACAUG 17 5908

HSV2-UL48-427 CCGCCAGGCACACAUGC 17 5909

HSV2-UL48-428 CGCCAGGCACACAUGCG 17 5910

HSV2-UL48-429 GCCGCAACCGCGACCUG 17 5911

HSV2-UL48-430 CCGCAACCGCGACCUGC 17 5912

HSV2-UL48-431 GCUGCGCACCACGAUCG 17 5913

HSV2-UL48-432 GCACCACGAUCGCGGAC 17 5914

HSV2-UL48-433 CCGCGAGACCGCGCGCC 17 5915

HSV2-UL48-434 UGAGCCGCGAGAUCCUA 17 5916

HSV2-UL48-435 GAGCCGCGAGAUCCUAU 17 5917

HSV2-UL48-436 ACGCCGAGCAGAUGAUG 17 5918

HSV2-UL48-437 CGGCCCGAUCUGUUCGA 17 5919

HSV2-UL48-438 CGGCCUCUGCUGCGACC 17 5920

HSV2-UL48-439 GCUGCGACCUGGAGAGC 17 5921

HSV2-UL48-440 GGAGAGCUGGCGCCAGU 17 5922

HSV2-UL48-441 CCCCUGAUGUUUAUCAA 17 5923

HSV2-UL48-442 ACGGAUCGCUCACCGUG 17 5924

HSV2-UL48-443 CGGAUCGCUCACCGUGC 17 5925

HSV2-UL48-444 GGAUCGCUCACCGUGCG 17 5926

HSV2-UL48-445 CGUGCGGGGAGUUCCCG 17 5927

HSV2-UL48-446 GCGGGGAGUUCCCGUGG 17 5928

HSV2-UL48-447 GAGUUCCCGUGGAGGCC 17 5929

HSV2-UL48-448 UGGAGGCCCGGCGACUG 17 5930

HSV2-UL48-449 GGAGGCCCGGCGACUGC 17 5931

HSV2-UL48-450 GCACCUGAACCUCCCGC 17 5932

HSV2-UL48-451 CCCGCUGGUGCGAAGUG 17 5933

HSV2-UL48-452 GCUGGUGCGAAGUGCGG 17 5934

HSV2-UL48-453 GGUGCGAAGUGCGGCGG 17 5935

HSV2-UL48-454 GCGAAGUGCGGCGGCGG 17 5936

HSV2-UL48-455 GCGGCGGCGGAGGAACC 17 5937

HSV2-UL48-456 CGGCGGCGGAGGAACCC 17 5938

HSV2-UL48-457 GGCGGCGGAGGAACCCG 17 5939

HSV2-UL48-458 GACCCCGCCCGUCCUGC 17 5940

HSV2-UL48-459 ACCCCGCCCGUCCUGCA 17 5941

HSV2-UL48-460 CGUCCUGCAGGGCAACC 17 5942 HSV2-UL48-461 AACCAGGCCCGCUCCUC 17 5943

HSV2-UL48-462 ACCAGGCCCGCUCCUCU 17 5944

HSV2-UL48-463 ACUUUAUGCUGCUGAUC 17 5945

HSV2-UL48-464 CUUUAUGCUGCU GAUCC 17 5946

HSV2-UL48-465 GCUGAUCCGGGCCAAGU 17 5947

HSV2-UL48-466 CUCCAGCGUCGCGACCU 17 5948

HSV2-UL48-467 CAGCGUCGCGACCUCGG 17 5949

HSV2-UL48-468 AGCGUCGCGACCUCGGA 17 5950

HSV2-UL48-469 AGGGCGAGUCCGUCAUG 17 5951

HSV2-11L48-470 GGGCGAGUCCGUCAUGC 17 5952

HSV2-UL48-471 GAGCACGCGUAUAGCCG 17 5953

HSV2-UL48-472 AGCACGCGUAUAGCCGC 17 5954

HSV2-UL48-473 ACGCGUAUAGCCGCGGG 17 5955

HSV2-UL48-474 CGGACCAGAAACAAUUA 17 5956

HSV2-UL48-475 UUACGGAUCGACAAUCG 17 5957

HSV2-UL48-476 UACGGAUCGACAAUCGA 17 5958

HSV2-UL48-477 GGGCCUGCUCGACCUCC 17 5959

HSV2-UL48-478 CGACGAUGACGCUCCUG 17 5960

HSV2-IIL48-479 CGAUGACGCUCCUGCGG 17 5961

HSV2-UL48-480 GACGCUCCUGCGGAGGC 17 5962

HSV2-UL48-481 ACGCUCCUGCGGAGGCC 17 5963

HSV2-UL48-482 UCCUGCGGAGGCCGGGC 17 5964

HSV2-UL48-483 UGCGGAGGCCGGGCUGG 17 5965

HSV2-UL48-484 CAUGUCGUUUCUCUCCG 17 5966

HSV2-UL48-485 AUGUCGUUUCUCUCCGC 17 5967

HSV2-UL48-486 CAUUACCGACGUCAGCC 17 5968

HSV2-UL48-487 AUUACCGACGUCAGCCU 17 5969

HSV2-UL48-488 GGGAGACGAACUCCGCC 17 5970

HSV2-UL48-489 GACGAACUCCGCCUGGA 17 5971

HSV2-UL48-490 ACUCCGCCUGGACGGCG 17 5972

HSV2-UL48-491 CCGCCUGG AC GGC G AGG 17 5973

HSV2-UL48-492 CCUGGACGGCGAGGAGG 17 5974

HSV2-UL48-493 GACGCCCGCCGACGCCC 17 5975

HSV2-UL48-494 CCUGGACGACUUCGACU 17 5976

HSV2-UL48-495 CUUCGACUUGGAGAUGC 17 5977

HSV2-UL48-496 UUCGACUUGGAGAUGCU 17 5978

HSV2-UL48-497 UCGACUUGGAGAUGCUG 17 5979

HSV2-UL48-498 CGACUUGG AG AU GCUGG 17 5980

HSV2-UL48-499 GGAGAUGCUGGGGGACG 17 5981

HSV2-UL48-500 CGUGGAGUCCCCCUCCC 17 5982

HSV2-UL48-501 GUGGAGUCCCCCUCCCC 17 5983

HSV2-UL48-502 CACGACCCCGUCUCGUA 17 5984

HSV2-UL48-503 ACGACCCCGUCUCGUAU 17 5985

HSV2-UL48-504 CGACCCCGUCUCGUAUG 17 5986

HSV2-UL48-505 CGUCUCGUAUGGGGCUU 17 5987

HSV2-UL48-506 GUAUGGGGCUUUGGACG 17 5988 HSV2-UL48-507 GAUGUUUACCGAUGCCA 17 5989

HSV2-UL48-508 AUGUUUACCGAUGCCAU 17 5990

HSV2-UL48-509 AUGGGCAUUGACGACUU 17 5991

HSV2-UL48-510 UGGGCAUUGACGACUUU 17 5992

HSV2-UL48-511 GGGCAUUGACGACUUUG 17 5993

HSV2-UL48-512 GGCAUUGACGACUUUGG 17 5994

HSV2-UL48-513 GC AUUG AC GACUUUGGG 17 5995

HSV2-UL48-514 + A AAGUCGUC A AU GCCCA 17 5996

HSV2-UL48-515 + GUCAAUGCCCAUGGCAU 17 5997

HSV2-UL48-516 + CAAAGCCCCAUACGAGA 17 5998

HSV2-UL48-517 + AAAGCCCCAUACGAGAC 17 5999

HSV2-UL48-518 + AAGCCCCAUACGAGACG 17 6000

HSV2-UL48-519 + CAUACGAGACGGGGUCG 17 6001

HSV2-UL48-520 + AUACGAGACGGGGUCGU 17 6002

HSV2-UL48-521 + GGGUCGUGGGUCAUUCC 17 6003

HSV2-UL48-522 + GGUCGUGGGUCAUUCCC 17 6004

HSV2-UL48-523 + GUCGUGGGUCAUUCCCG 17 6005

HSV2-UL48-524 + GUGGGUCAUUCCCGGGG 17 6006

HSV2-UL48-525 + UGGGUCAUUCCCGGGGA 17 6007

HSV2-UL48-526 + GGGUCAUUCCCGGGGAG 17 6008

HSV2-UL48-527 + GGUCAUUCCCGGGGAGG 17 6009

HSV2-UL48-528 + CCAAGUCGAAGUCGUCC 17 6010

HSV2-UL48-529 + CAAGUCGAAGUCGUCCA 17 6011

HSV2-UL48-530 + GAAGUCGUCCAGGGCGU 17 6012

HSV2-UL48-531 + GUCGUCCAGGGCGUCGG 17 6013

HSV2-UL48-532 + UCGUCCAGGGCGUCGGC 17 6014

HSV2-UL48-533 + CCACCUCCUCGCCGUCC 17 6015

HSV2-UL48-534 + CCUCCUCGCCGUCCAGG 17 6016

HSV2-UL48-535 + GGCGGAGUUCGUCUCCC 17 6017

HSV2-UL48-536 + GUCUCCCAGGCUGACGU 17 6018

HSV2-UL48-537 + CAGGCUGACGUCGGUAA 17 6019

HSV2-UL48-538 + AGGCUGACGUCGGUAAU 17 6020

HSV2-UL48-539 + GGCUGACGUCGGUAAUG 17 6021

HSV2-UL48-540 + GCUGACGUCGGUAAUGG 17 6022

HSV2-UL48-541 + GACGUCGGUAAUGGGGG 17 6023

HSV2-UL48-542 + GUCGGUAAUGGGGGCGG 17 6024

HSV2-UL48-543 + GGUAAUGGGGGCGGUGG 17 6025

HSV2-UL48-544 + CGGUGGUGGACAGUCUG 17 6026

HSV2-UL48-545 + GGUGGUGGACAGUCUGC 17 6027

HSV2-UL48-546 + GUGGUGGACAGUCUGCG 17 6028

HSV2-UL48-547 + UGGUGG AC AGUC UGCGG 17 6029

HSV2-UL48-548 + GCGGGGGCGUUGUCCCG 17 6030

HSV2-UL48-549 + GAGAGAAACGACAUGCG 17 6031

HSV2-UL48-550 + GCGCGGCGCCACCAGCC 17 6032

HSV2-UL48-551 + ACCAGCCCGGCCUCCGC 17 6033

HSV2-UL48-552 + GGAGCGUCAUCGUCGUC 17 6034 HSV2-UL48-553 + GAGCGUC AUCGU CGUCC 17 6035

HSV2-UL48-554 + CGUCAUCGUCGUCCGGG 17 6036

HSV2-UL48-555 + CGUCCGGGAGGUCGAGC 17 6037

HSV2-UL48-556 + CGAUCCGUAAUUGUUUC 17 6038

HSV2-UL48-557 + UGUUUCUGGUCCGCCCG 17 6039

HSV2-UL48-558 + CGCGUGCUCCCGCAUGA 17 6040

HSV2-UL48-559 + GACGGACUCGCCCUCCG 17 6041

HSV2-UL48-560 + CUCCGAGGUCGCGACGC 17 6042

HSV2-UL48-561 + GGAGUACGAGUCCAACU 17 6043

HSV2-UL48-562 + ACGAGUCCAACUUGGCC 17 6044

HSV2-UL48-563 + CAGCAUAAAGUACCCAG 17 6045

HSV2-UL48-564 + UAAAGUACCCAGAGGAG 17 6046

HSV2-UL48-565 + AAAGUACCCAGAGGAGC 17 6047

HSV2-UL48-566 + ACCCAGAGGAGCGGGCC 17 6048

HSV2-UL48-567 + GGGCCUGGUUGCCCUGC 17 6049

HSV2-UL48-568 + CUGGUUGCCCUGCAGGA 17 6050

HSV2-UL48-569 + UGGUUGCCCUGCAGGAC 17 6051

HSV2-UL48-570 + UUGCCCUGCAGGACGGG 17 6052

HSV2-UL48-571 + UGCCCUGCAGGACGGGC 17 6053

HSV2-UL48-572 + GCCCUGCAGGACGGGCG 17 6054

HSV2-UL48-573 + GGACGGGCGGGGUCGUG 17 6055

HSV2-UL48-574 + GACGGGCGGGGUCGUGA 17 6056

HSV2-UL48-575 + ACGGGCGGGGUCGUGAG 17 6057

HSV2-UL48-576 + GGUCGUGAGGGGCGCCC 17 6058

HSV2-UL48-577 + GUCGUGAGGGGCGCCCC 17 6059

HSV2-UL48-578 + GCCGCACUUCGCACCAG 17 6060

HSV2-UL48-579 + CCGCACUUCGCACCAGC 17 6061

HSV2-UL48-580 + CACUUCGCACCAGCGGG 17 6062

HSV2-UL48-581 + GCACCAGCGGGAGGUUC 17 6063

HSV2-UL48-582 + UCAGGUGCUCGCGAAUG 17 6064

HSV2-UL48-583 + UUAGCUCCCGCAGUCGC 17 6065

HSV2-UL48-584 + UAGCUCCCGCAGUCGCC 17 6066

HSV2-UL48-585 + CAGUCGCCGGGCCUCCA 17 6067

HSV2-UL48-586 + AGUCGCCGGGCCUCCAC 17 6068

HSV2-UL48-587 + CACGGGAACUCCCCGCA 17 6069

HSV2-UL48-588 + AUCCGUUGAUAAACAUC 17 6070

HSV2-UL48-589 + UCCGUUGAUAAACAUCA 17 6071

HSV2-UL48-590 + CCGUUGAUAAACAUCAG 17 6072

HSV2-UL48-591 + GAAACAGACACGCCAAC 17 6073

HSV2-UL48-592 + ACUGGCGCCAGCUCUCC 17 6074

HSV2-UL48-593 + UCUCCAGGUCGCAGCAG 17 6075

HSV2-UL48-594 + GAGGCCGUCGAACAGAU 17 6076

HSV2-UL48-595 + AGGCCGUCGAACAGAUC 17 6077

HSV2-UL48-596 + GGGCCGCAUCAUCUGCU 17 6078

HSV2-UL48-597 + CAUCUGCUCGGCGUACG 17 6079

HSV2-UL48-598 + CGGCGUACGCGGCCCAU 17 6080 HSV2-UL48-599 + CGGCCCAUAGGAUCUCG 17 6081

HSV2-UL48-600 + UCUCGCGGCUCAGAAAG 17 6082

HSV2-UL48-601 + GGUAUAGAUGCAGAAAC 17 6083

HSV2-UL48-602 + GAAACAGGACGCGCGCC 17 6084

HSV2-UL48-603 + GACGCGCGCCAGGCGCG 17 6085

HSV2-UL48-604 + CCAGGCGCGCGGUCUCG 17 6086

HSV2-UL48-605 + GUACCUGUCCGCGAUCG 17 6087

HSV2-UL48-606 + UGCGCAGCAUCUCCCGC 17 6088

HSV2-UL48-607 + GCAUCUCCCGCAGGUCG 17 6089

HSV2-UL48-608 + CCCGCAGGUCGCGGUUG 17 6090

HSV2-UL48-609 + GGCCCCGCAUGUGUGCC 17 6091

HSV2-UL48-610 + CCCGCAUGUGUGCCUGG 17 6092

HSV2-UL48-611 + GUGUAGCUGCCGAACGC 17 6093

HSV2-UL48-612 + GCCGAACGCUGGCGCGC 17 6094

HSV2-UL48-613 + CGCUGGCGCGCAGGUAC 17 6095

HSV2-UL48-614 + CGCGCAGGUACCGGUAC 17 6096

HSV2-UL48-615 + GCGCAGGUACCGGUACA 17 6097

Table 3D provides exemplary targeting domains for knocking out the UL48 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL48 gene. In an embodiment, the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks. In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands and that are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3D

HSV2-UL48-624 CGCUGUACGCCACCGGUCGG 20 6103

HSV2-UL48-625 UUAACCGUCUCCUCGACGAU 20 6104

HSV2-UL48-626 UCGACGAUCUGGGCUUCAGC 20 6105

HSV2-UL48-627 CUGUGUACCAUGCUAGAUAC 20 6106

HSV2-UL48-19 UGUGUACCAUGCUAGAUACC 20 5604

HSV2-UL48-629 UACCAUGCUAGAUACCUGGA 20 6107

HSV2-UL48-630 CCAUGCUAGAUACCUGGAAC 20 6108

HSV2-UL48-631 CUGGAACGAGGACCUGUUCU 20 6109

HSV2-UL48-632 CCGACCAACGCCGACAUGUA 20 6110

HSV2-UL48-23 CGACCAACGCCGACAUGUAC 20 5607

HSV2-UL48-24 GACCAACGCCGACAUGUACC 20 5500

HSV2-UL48-635 CUGCCCAGCGACGUGAUCGA 20 6111

HSV2-UL48-25 UGCCCAGCGACGUGAUCGAC 20 5501

HSV2-UL48-26 GCCCAGCGACGUGAUCGACU 20 5608

HSV2-UL48-27 CCCAGCGACGUGAUCGACUG 20 5609

HSV2-UL48-28 CCAGCGACGUGAUCGACUGG 20 5610

HSV2-UL48-640 CUGGGGGGAUGCGCACGUCC 20 6112

HSV2-UL48-641 CACCCUGCCCGCCACCCGCG 20 6113

HSV2-UL48-642 CGACGAGCUGCCUUCGUACU 20 6114

HSV2-UL48-643 CAUGGCGCAGUUUUUCCGCG 20 6115

HSV2-UL48-644 CAGUUUUUCCGCGGUGAGCU 20 6116

HSV2-UL48-645 UUCCGCGGUGAGCUGCGGGC 20 6117

HSV2-UL48-37 UCCGCGGUGAGCUGCGGGCG 20 5611

HSV2-UL48-38 CCGCGGUGAGCUGCGGGCGC 20 5612

HSV2-UL48-648 GCGGUGAGCUGCGGGCGCGG 20 6118

HSV2-UL48-39 CGGUGAGCUGCGGGCGCGGG 20 5613

HSV2-UL48-650 GUGAGCUGCGGGCGCGGGAG 20 6119

HSV2-UL48-651 CGGGCGCGGGAGGAGAGCUA 20 6120

HSV2-UL48-652 UGGUCGACGAUCUGUUU 17 6121

HSV2-UL48-653 ACGAUCUGUUUGCGGAC 17 6122

HSV2-UL48-654 UCUGUUUGCGGACGCGG 17 6123

HSV2-UL48-117 CUGUUUGCGGACGCGGA 17 5486

HSV2-UL48-656 ACCGCCCCCCAGGCCAG 17 6124

HSV2-UL48-121 CCGCCCCCCAGGCCAGC 17 5655

HSV2-UL48-122 CGCCCCCCAGGCCAGCC 17 5656

HSV2-UL48-659 GGCCAGCCGGGGGUCCC 17 6125

HSV2-UL48-660 UGUACGCCACCGGUCGG 17 6126

HSV2-UL48-661 ACCGUCUCCUCGACGAU 17 6127

HSV2-UL48-662 ACGAUCUGGGCUUCAGC 17 6128

HSV2-UL48-663 UGUACCAUGCUAGAUAC 17 6129

HSV2-UL48-133 GUACCAUGCUAGAUACC 17 5662

HSV2-UL48-665 CAUGCUAGAUACCUGGA 17 6130

HSV2-UL48-666 UGCUAGAUACCUGGAAC 17 6131

HSV2-UL48-667 GAACGAGGACCUGUUCU 17 6132

HSV2-UL48-668 ACCAACGCCGACAUGUA 17 6133

HSV2-UL48-137 CCAACGCCGACAUGUAC 17 5665 HSV2-UL48-138 CAACGCCGACAUGUACC 17 5502

HSV2-UL48-671 CCCAGCGACGUGAUCGA 17 6134

HSV2-UL48-139 CCAGCGACGUGAUCGAC 17 5503

HSV2-UL48-140 CAGCGACGUGAUCGACU 17 5666

HSV2-UL48-141 AGCGACGUGAUCGACUG 17 5667

HSV2-UL48-142 GCGACGUGAUCGACUGG 17 5668

HSV2-UL48-676 GGGGGAUGCGCACGUCC 17 6135

HSV2-UL48-677 CCUGCCCGCCACCCGCG 17 6136

HSV2-UL48-678 CGAGCUGCCUUCGUACU 17 6137

HSV2-UL48-679 GGCGCAGUUUUUCCGCG 17 6138

HSV2-UL48-680 UUUUUCCGCGGUGAGCU 17 6139

HSV2-UL48-681 CGCGGUGAGCUGCGGGC 17 6140

HSV2-UL48-151 GCGGUGAGCUGCGGGCG 17 5669

HSV2-UL48-152 CGGUGAGCUGCGGGCGC 17 5670

HSV2-UL48-684 GUGAGCUGCGGGCGCGG 17 6141

HSV2-UL48-153 UGAGCUGCGGGCGCGGG 17 5671

HSV2-UL48-686 AGCUGCGGGCGCGGGAG 17 6142

HSV2-UL48-687 GCGCGGGAGGAGAGCUA 17 6143

HSV2-UL48-688 + GGCUGGCCUGGGGGGCGGUG 20 6144

HSV2-UL48-109 + UGGGACCCCCGGCUGGCCUG 20 5647

HSV2-UL48-108 + UUGGGACCCCCGGCUGGCCU 20 5646

HSV2-UL48-107 + CUUGGGACCCCCGGCUGGCC 20 5645

HSV2-UL48-692 + UCUUGGGACCCCCGGCUGGC 20 6145

HSV2-UL48-103 + AGGGGCGGCUGGGGUGUUCU 20 5642

HSV2-UL48-694 + GAGGGGCGGCUGGGGUGUUC 20 6146

HSV2-UL48-100 + GCGUACAGCGGAGGGGCGGC 20 5640

HSV2-UL48-696 + GGCGUACAGCGGAGGGGCGG 20 6147

HSV2-UL48-96 + CGACCGGUGGCGUACAGCGG 20 5638

HSV2-UL48-698 + CCGACCGGUGGCGUACAGCG 20 6148

HSV2-UL48-95 + AGCCGACCGGUGGCGUACAG 20 5581

HSV2-UL48-700 + CAGCCGACCGGUGGCGUACA 20 6149

HSV2-UL48-701 + AUGGGCGGCGAGGGCAUCAG 20 6150

HSV2-UL48-702 + GGGGGACGGGCAUGGGCGGC 20 6151

HSV2-UL48-703 + GGGGGGGACGGGCAUGGGCG 20 6152

HSV2-UL48-704 + GGGCCGCGGGGGGGACGGGC 20 6153

HSV2-UL48-705 + UAAACAGGGCCGCGGGGGGG 20 6154

HSV2-UL48-82 + CGGUUAAACAGGGCCGCGGG 20 5572

HSV2-UL48-81 + ACGGUUAAACAGGGCCGCGG 20 5571

HSV2-UL48-80 + GACGGUUAAACAGGGCCGCG 20 5570

HSV2-UL48-79 + AGACGGUUAAACAGGGCCGC 20 5569

HSV2-UL48-78 + GAGACGGUUAAACAGGGCCG 20 5568

HSV2-UL48-711 + GGAGACGGUUAAACAGGGCC 20 6155

HSV2-UL48-712 + UCGUCGAGGAGACGGUUAAA 20 6156

HSV2-UL48-74 + CGCUGAAGCCCAGAUCGUCG 20 5625

HSV2-UL48-714 + GCGCUGAAGCCCAGAUCGUC 20 6157

HSV2-UL48-715 + CCGCGCUGAAGCCCAGAUCG 20 6158 HSV2-UL48-716 + UACACAGCGCGGGACCCGCG 20 6159

HSV2-UL48-72 + AUCUAGCAUGGUACACAGCG 20 5624

HSV2-UL48-718 + UAUCUAGCAUGGUACACAGC 20 6160

HSV2-UL48-719 + CGGCGUUGGUCGGGAACCCA 20 6161

HSV2-UL48-720 + GUCGGCGUUGGUCGGGAACC 20 6162

HSV2-UL48-68 + GGUACAUGUCGGCGUUGGUC 20 5557

HSV2-UL48-67 + CGGUACAUGUCGGCGUUGGU 20 5556

HSV2-UL48-723 + CCGGUACAUGUCGGCGUUGG 20 6163

HSV2-UL48-724 + ACGUCGCUGGGCAGCGUCGA 20 6164

HSV2-UL48-725 + CCCCCCAGUCGAUCACGUCG 20 6165

HSV2-UL48-60 + AUGUCGAUCGGGGAGCGCUC 20 5541

HSV2-UL48-59 + AAUGUCGAUCGGGGAGCGCU 20 5540

HSV2-UL48-728 + GAAUGUCGAUCGGGGAGCGC 20 6166

HSV2-UL48-58 + GUGGGCGCGAAUGUCGAUCG 20 5539

HSV2-UL48-57 + CGUGGGCGCGAAUGUCGAUC 20 5538

HSV2-UL48-56 + CCGUGGGCGCGAAUGUCGAU 20 5537

HSV2-UL48-732 + GCCGUGGGCGCGAAUGUCGA 20 6167

HSV2-UL48-733 + AACGCCACGUCGCCGUGGGC 20 6168

HSV2-UL48-734 + GUGGGGAACGCCACGUCGCC 20 6169

HSV2-UL48-53 + CGCGGGUGGCGGGCAGGGUG 20 5619

HSV2-UL48-52 + UCGCGGGUGGCGGGCAGGGU 20 5618

HSV2-UL48-51 + GUCGCGGGUGGCGGGCAGGG 20 5617

HSV2-UL48-738 + CGUCGCGGGUGGCGGGCAGG 20 6170

HSV2-UL48-739 + AGCUCGUCGCGGGUGGCGGG 20 6171

HSV2-UL48-740 + AAGGCAGCUCGUCGCGGGUG 20 6172

HSV2-UL48-741 + UAGUACGAAGGCAGCUCGUC 20 6173

HSV2-UL48-742 + CUGCGCCAUGGCCUCGUAGU 20 6174

HSV2-UL48-41 + CCCGCGCCCGCAGCUCACCG 20 5615

HSV2-UL48-744 + UCCCGCGCCCGCAGCUCACC 20 6175

HSV2-UL48-745 + UGGCCUGGGGGGCGGUG 17 6176

HSV2-UL48-223 + GACCCCCGGCUGGCCUG 17 5705

HSV2-UL48-222 + GGACCCCCGGCUGGCCU 17 5704

HSV2-UL48-221 + GGGACCCCCGGCUGGCC 17 5703

HSV2-UL48-749 + UGGGACCCCCGGCUGGC 17 6177

HSV2-UL48-217 + GGCGGCUGGGGUGUUCU 17 5700

HSV2-UL48-751 + GGGCGGCUGGGGUGUUC 17 6178

HSV2-UL48-214 + UACAGCGGAGGGGCGGC 17 5698

HSV2-UL48-753 + GUACAGCGGAGGGGCGG 17 6179

HSV2-UL48-210 + CCGGUGGCGUACAGCGG 17 5696

HSV2-UL48-755 + ACCGGUGGCGUACAGCG 17 6180

HSV2-UL48-209 + CGACCGGUGGCGUACAG 17 5585

HSV2-UL48-757 + CCGACCGGUGGCGUACA 17 6181

HSV2-UL48-758 + GGCGGCGAGGGCAUCAG 17 6182

HSV2-UL48-759 + GGACGGGCAUGGGCGGC 17 6183

HSV2-UL48-760 + GGGGACGGGCAUGGGCG 17 6184

HSV2-UL48-761 + CCGCGGGGGGGACGGGC 17 6185 HSV2-UL48-762 + ACAGGGCCGCGGGGGGG 17 6186

HSV2-UL48-196 + UUAAACAGGGCCGCGGG 17 5579

HSV2-UL48-195 + GUUAAACAGGGCCGCGG 17 5578

HSV2-UL48-194 + GGUUAAACAGGGCCGCG 17 5577

HSV2-UL48-193 + CGGUUAAACAGGGCCGC 17 5576

HSV2-UL48-192 + ACGGUUAAACAGGGCCG 17 5575

HSV2-UL48-768 + GACGGUUAAACAGGGCC 17 6187

HSV2-UL48-769 + UCGAGGAGACGGUUAAA 17 6188

HSV2-UL48-188 + UGAAGCCCAGAUCGUCG 17 5683

HSV2-UL48-771 + CUGAAGCCCAGAUCGUC 17 6189

HSV2-UL48-772 + CGCUGAAGCCCAGAUCG 17 6190

HSV2-UL48-773 + ACAGCGCGGGACCCGCG 17 6191

HSV2-UL48-186 + UAGCAUGGUACACAGCG 17 5682

HSV2-UL48-775 + CUAGCAUGGUACACAGC 17 6192

HSV2-UL48-776 + CGUUGGUCGGGAACCCA 17 6193

HSV2-UL48-777 + GGCGUUGGUCGGGAACC 17 6194

HSV2-UL48-182 + ACAUGUCGGCGUUGGUC 17 5561

HSV2-UL48-181 + UACAUGUCGGCGUUGGU 17 5560

HSV2-UL48-780 + GUACAUGUCGGCGUUGG 17 6195

HSV2-UL48-781 + UCGCUGGGCAGCGUCGA 17 6196

HSV2-UL48-782 + CCCAGUCGAUCACGUCG 17 6197

HSV2-UL48-174 + UCGAUCGGGGAGCGCUC 17 5550

HSV2-UL48-173 + GUCGAUCGGGGAGCGCU 17 5549

HSV2-UL48-785 + UGUCGAUCGGGGAGCGC 17 6198

HSV2-UL48-172 + GGCGCGAAUGUCGAUCG 17 5548

HSV2-UL48-171 + GGGCGCGAAUGUCGAUC 17 5547

HSV2-UL48-170 + UGGGCGCGAAUGUCGAU 17 5546

HSV2-UL48-789 + GUGGGCGCGAAUGUCGA 17 6199

HSV2-UL48-790 + GCCACGUCGCCGUGGGC 17 6200

HSV2-UL48-791 + GGGAACGCCACGUCGCC 17 6201

HSV2-UL48-167 + GGGUGGCGGGCAGGGUG 17 5677

HSV2-UL48-166 + CGGGUGGCGGGCAGGGU 17 5676

HSV2-UL48-165 + GCGGGUGGCGGGCAGGG 17 5675

HSV2-UL48-795 + CGCGGGUGGCGGGCAGG 17 6202

HSV2-UL48-796 + UCGUCGCGGGUGGCGGG 17 6203

HSV2-UL48-797 + GCAGCUCGUCGCGGGUG 17 6204

HSV2-UL48-798 + UACGAAGGCAGCUCGUC 17 6205

HSV2-UL48-799 + CGCCAUGGCCUCGUAGU 17 6206

HSV2-UL48-155 + GCGCCCGCAGCUCACCG 17 5673

HSV2-UL48-801 + CGCGCCCGCAGCUCACC 17 6207

Table 3E provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence, but downstream of the first 500bp of the UL48 gene. In an embodiment, the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks. In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands and that are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3E

HSV2-UL48-261 AGUGCGGCGGCGGAGGAACC 20 5743

HSV2-UL48-835 UCACGACCCCGCCCGUCCUG 20 6228

HSV2-UL48-836 GGGCAACCAGGCCCGCUCCU 20 6229

HSV2-UL48-837 GGGUACUUUAUGCUGCUGAU 20 6230

HSV2-UL48-838 UGCUGCUGAUCCGGGCCAAG 20 6231

HSV2-UL48-839 CGUACUCCAGCGUCGCGACC 20 6232

HSV2-UL48-272 GUACUCCAGCGUCGCGACCU 20 5754

HSV2-UL48-841 ACUCCAGCGUCGCGACCUCG 20 6233

HSV2-UL48-842 CAGCGUCGCGACCUCGGAGG 20 6234

HSV2-UL48-843 UCGGAGGGCGAGUCCGUCAU 20 6235

HSV2-UL48-275 CGGAGGGCGAGUCCGUCAUG 20 5757

HSV2-UL48-276 GGAGGGCGAGUCCGUCAUGC 20 5758

HSV2-UL48-846 GCGGGAGCACGCGUAUAGCC 20 6236

HSV2-UL48-847 GAGCACGCGUAUAGCCGCGG 20 6237

HSV2-UL48-848 GCGUAUAGCCGCGGGCGGAC 20 6238

HSV2-UL48-849 CGGGCGGACCAGAAACAAUU 20 6239

HSV2-UL48-850 AAACAAUUACGGAUCGACAA 20 6240

HSV2-UL48-851 ACAAUUACGGAUCGACAAUC 20 6241

HSV2-UL48-852 UCGAGGGCCUGCUCGACCUC 20 6242

HSV2-UL48-853 CGGACGACGAUGACGCUCCU 20 6243

HSV2-UL48-284 GGACGACGAUGACGCUCCUG 20 5766

HSV2-UL48-855 CGAUGACGCUCCUGCGGAGG 20 6244

HSV2-UL48-856 CGCGCAUGUCGUUUCUCUCC 20 6245

HSV2-UL48-290 GCGCAUGUCGUUUCUCUCCG 20 5772

HSV2-UL48-858 CCCCCAUUACCGACGUCAGC 20 6246

HSV2-UL48-292 CCCCAUUACCGACGUCAGCC 20 5774

HSV2-UL48-293 CCCAUUACCGACGUCAGCCU 20 5775

HSV2-UL48-861 UACCGACGUCAGCCUGGGAG 20 6247

HSV2-UL48-862 GCCUGGGAGACGAACUCCGC 20 6248

HSV2-UL48-863 AGACGAACUCCGCCUGGACG 20 6249

HSV2-UL48-864 ACGAACUCCGCCUGGACGGC 20 6250

HSV2-UL48-296 CGAACUCCGCCUGGACGGCG 20 5778

HSV2-UL48-866 UCCGCCUGGACGGCGAGGAG 20 6251

HSV2-UL48-867 AUAUGACGCCCGCCGACGCC 20 6252

HSV2-UL48-868 ACGCCCUGGACGACUUCGAC 20 6253

HSV2-UL48-300 CGCCCUGGACGACUUCGACU 20 5782

HSV2-UL48-870 ACGACUUCGACUUGGAGAUG 20 6254

HSV2-UL48-301 CGACUUCGACUUGGAGAUGC 20 5783

HSV2-UL48-302 GACUUCGACUUGGAGAUGCU 20 5784

HSV2-UL48-303 ACUUCGACUUGGAGAUGCUG 20 5785

HSV2-UL48-874 ACUUGGAGAUGCUGGGGGAC 20 6255

HSV2-UL48-305 CUUGGAGAUGCUGGGGGACG 20 5787

HSV2-UL48-876 GGGACGUGGAGUCCCCCUCC 20 6256

HSV2-UL48-306 GGACGUGGAGUCCCCCUCCC 20 5788

HSV2-UL48-307 GACGUGGAGUCCCCCUCCCC 20 5789

HSV2-UL48-879 GACCCACGACCCCGUCUCGU 20 6257 HSV2-UL48-308 ACCCACGACCCCGUCUCGUA 20 5790

HSV2-UL48-881 ACCCCGUCUCGUAUGGGGCU 20 6258

HSV2-UL48-882 UCUCGUAUGGGGCUUUGGAC 20 6259

HSV2-UL48-883 GGCUUUGGACGUGGACGAUU 20 6260

HSV2-UL48-884 GGACGUGGACGAUUUUGAGU 20 6261

HSV2-UL48-885 AACAGAUGUUUACCGAUGCC 20 6262

HSV2-UL48-886 UGCCAUGGGCAUUGACGACU 20 6263

HSV2-UL48-315 GCCAUGGGCAUUGACGACUU 20 5797

HSV2-UL48-316 CCAUGGGCAUUGACGACUUU 20 5798

HSV2-UL48-317 CAUGGGCAUUGACGACUUUG 20 5799

HSV2-UL48-890 CACCGCCAGGCACACAU 17 6264

HSV2-UL48-426 ACCGCCAGGCACACAUG 17 5908

HSV2-UL48-892 GGCCGCAACCGCGACCU 17 6265

HSV2-UL48-429 GCCGCAACCGCGACCUG 17 5911

HSV2-UL48-430 CCGCAACCGCGACCUGC 17 5912

HSV2-UL48-895 UGCUGCGCACCACGAUC 17 6266

HSV2-UL48-896 CGCGGACAGGUACUACC 17 6267

HSV2-UL48-897 UGCAUCUAUACCUCUUU 17 6268

HSV2-UL48-898 AUACCUCUUUCUGAGCC 17 6269

HSV2-UL48-899 CUGAGCCGCGAGAUCCU 17 6270

HSV2-UL48-900 CCUAUGGGCCGCGUACG 17 6271

HSV2-UL48-901 ACGGCCUCUGCUGCGAC 17 6272

HSV2-UL48-438 CGGCCUCUGCUGCGACC 17 5920

HSV2-UL48-903 GCCUCUGCUGCGACCUG 17 6273

HSV2-UL48-904 GCCCCUGAUGUUUAUCA 17 6274

HSV2-UL48-905 AACGGAUCGCUCACCGU 17 6275

HSV2-UL48-442 ACGGAUCGCUCACCGUG 17 5924

HSV2-UL48-443 CGGAUCGCUCACCGUGC 17 5925

HSV2-UL48-444 GGAUCGCUCACCGUGCG 17 5926

HSV2-UL48-909 CCGUGCGGGGAGUUCCC 17 6276

HSV2-UL48-445 CGUGCGGGGAGUUCCCG 17 5927

HSV2-UL48-911 GUGGAGGCCCGGCGACU 17 6277

HSV2-UL48-448 UGGAGGCCCGGCGACUG 17 5930

HSV2-UL48-449 GGAGGCCCGGCGACUGC 17 5931

HSV2-UL48-914 GGAGCUAAACCACAUUC 17 6278

HSV2-UL48-915 ACCACAUUCGCGAGCAC 17 6279

HSV2-UL48-916 CUGAACCUCCCGCUGGU 17 6280

HSV2-UL48-917 UGGUGCGAAGUGCGGCG 17 6281

HSV2-UL48-453 GGUGCGAAGUGCGGCGG 17 5935

HSV2-UL48-919 UGCGAAGUGCGGCGGCG 17 6282

HSV2-UL48-454 GCGAAGUGCGGCGGCGG 17 5936

HSV2-UL48-921 UGCGGCGGCGGAGGAAC 17 6283

HSV2-UL48-455 GCGGCGGCGGAGGAACC 17 5937

HSV2-UL48-923 CGACCCCGCCCGUCCUG 17 6284

HSV2-UL48-924 CAACCAGGCCCGCUCCU 17 6285

HSV2-UL48-925 UACUUUAUGCUGCUGAU 17 6286 HSV2-UL48-926 UGCUGAUCCGGGCCAAG 17 6287

HSV2-UL48-927 ACUCCAGCGUCGCGACC 17 6288

HSV2-UL48-466 CUCCAGCGUCGCGACCU 17 5948

HSV2-UL48-929 CCAGCGUCGCGACCUCG 17 6289

HSV2-UL48-930 CGUCGCGACCUCGGAGG 17 6290

HSV2-UL48-931 GAGGGCGAGUCCGUCAU 17 6291

HSV2-UL48-469 AGGGCGAGUCCGUCAUG 17 5951

HSV2-UL48-470 GGGCGAGUCCGUCAUGC 17 5952

HSV2-UL48-934 GGAGCACGCGUAUAGCC 17 6292

HSV2-UL48-935 CACGCGUAUAGCCGCGG 17 6293

HSV2-UL48-936 UAUAGCCGCGGGCGGAC 17 6294

HSV2-UL48-937 GCGGACCAGAAACAAUU 17 6295

HSV2-UL48-938 CAAUUACGGAUCGACAA 17 6296

HSV2-UL48-939 AUUACGGAUCGACAAUC 17 6297

HSV2-UL48-940 AGGGCCUGCUCGACCUC 17 6298

HSV2-UL48-941 ACGACGAUGACGCUCCU 17 6299

HSV2-UL48-478 CGACGAUGACGCUCCUG 17 5960

HSV2-UL48-943 UGACGCUCCUGCGGAGG 17 6300

HSV2-UL48-944 GCAUGUCGUUUCUCUCC 17 6301

HSV2-UL48-484 CAUGUCGUUUCUCUCCG 17 5966

HSV2-UL48-946 CCAUUACCGACGUCAGC 17 6302

HSV2-UL48-486 CAUUACCGACGUCAGCC 17 5968

HSV2-UL48-487 AUUACCGACGUCAGCCU 17 5969

HSV2-UL48-949 CGACGUCAGCCUGGGAG 17 6303

HSV2-UL48-950 UGGGAGACGAACUCCGC 17 6304

HSV2-UL48-951 CGAACUCCGCCUGGACG 17 6305

HSV2-UL48-952 AACUCCGCCUGGACGGC 17 6306

HSV2-UL48-490 ACUCCGCCUGGACGGCG 17 5972

HSV2-UL48-954 GCCUGGACGGCGAGGAG 17 6307

HSV2-UL48-955 UGACGCCCGCCGACGCC 17 6308

HSV2-UL48-956 CCCUGGACGACUUCGAC 17 6309

HSV2-UL48-494 CCUGGACGACUUCGACU 17 5976

HSV2-UL48-958 ACUUCGACUUGGAGAUG 17 6310

HSV2-UL48-495 CUUCGACUUGGAGAUGC 17 5977

HSV2-UL48-496 UUCGACUUGGAGAUGCU 17 5978

HSV2-UL48-497 UCGACUUGGAGAUGCUG 17 5979

HSV2-UL48-962 UGGAGAUGCUGGGGGAC 17 6311

HSV2-UL48-499 GGAGAUGCUGGGGGACG 17 5981

HSV2-UL48-964 ACGUGGAGUCCCCCUCC 17 6312

HSV2-UL48-500 CGUGGAGUCCCCCUCCC 17 5982

HSV2-UL48-501 GUGGAGUCCCCCUCCCC 17 5983

HSV2-UL48-967 CCACGACCCCGUCUCGU 17 6313

HSV2-UL48-502 CACGACCCCGUCUCGUA 17 5984

HSV2-UL48-969 CCGUCUCGUAUGGGGCU 17 6314

HSV2-UL48-970 CGUAUGGGGCUUUGGAC 17 6315

HSV2-UL48-971 UUUGGACGUGGACGAUU 17 6316 HSV2-UL48-972 CGUGGACGAUUUUGAGU 17 6317

HSV2-UL48-973 AGAUGUUUACCGAUGCC 17 6318

HSV2-UL48-974 CAUGGGCAUUGACGACU 17 6319

HSV2-UL48-509 AUGGGCAUUGACGACUU 17 5991

HSV2-UL48-510 UGGGCAUUGACGACUUU 17 5992

HSV2-UL48-511 GGGCAUUGACGACUUUG 17 5993

HSV2-UL48-978 + GCGCAGGUACCGGUACAGGG 20 6320

HSV2-UL48-979 + CUGGCGCGCAGGUACCGGUA 20 6321

HSV2-UL48-980 + UGUGCCUGGCGGUGUAGCUG 20 6322

HSV2-UL48-981 + AAGAGGUAUAGAUGCAGAAA 20 6323

HSV2-UL48-982 + CUCAGAAAGAGGUAUAGAUG 20 6324

HSV2-UL48-983 + AUAGGAUCUCGCGGCUCAGA 20 6325

HSV2-UL48-984 + GCCCAUAGGAUCUCGCGGCU 20 6326

HSV2-UL48-985 + UGCUCGGCGUACGCGGCCCA 20 6327

HSV2-UL48-986 + AGCAGAGGCCGUCGAACAGA 20 6328

HSV2-UL48-987 + CCAGGUCGCAGCAGAGGCCG 20 6329

HSV2-UL48-988 + GCCAGCUCUCCAGGUCGCAG 20 6330

HSV2-UL48-989 + CCGUUGAUAAACAUCAGGGG 20 6331

HSV2-UL48-394 + GCGAUCCGUUGAUAAACAUC 20 5876

HSV2-UL48-991 + AGCGAUCCGUUGAUAAACAU 20 6332

HSV2-UL48-992 + CCACGGGAACUCCCCGCACG 20 6333

HSV2-UL48-392 + CGCAGUCGCCGGGCCUCCAC 20 5874

HSV2-UL48-391 + CCGCAGUCGCCGGGCCUCCA 20 5873

HSV2-UL48-995 + CCCGCAGUCGCCGGGCCUCC 20 6334

HSV2-UL48-996 + UGGUUUAGCUCCCGCAGUCG 20 6335

HSV2-UL48-997 + AGCGGGAGGUUCAGGUGCUC 20 6336

HSV2-UL48-385 + CCGCCGCACUUCGCACCAGC 20 5867

HSV2-UL48-384 + GCCGCCGCACUUCGCACCAG 20 5866

HSV2-UL48-1000 + CGCCGCCGCACUUCGCACCA 20 6337

HSV2-UL48-1001 + GCGGGGUCGUGAGGGGCGCC 20 6338

HSV2-UL48-379 + GCAGGACGGGCGGGGUCGUG 20 5861

HSV2-UL48-1003 + UGCAGGACGGGCGGGGUCGU 20 6339

HSV2-UL48-1004 + CCUGCAGGACGGGCGGGGUC 20 6340

HSV2-UL48-376 + UGGUUGCCCUGCAGGACGGG 20 5858

HSV2-UL48-1006 + CUGGUUGCCCUGCAGGACGG 20 6341

HSV2-UL48-1007 + GGGCCUGGUUGCCCUGCAGG 20 6342

HSV2-UL48-1008 + GAGCGGGCCUGGUUGCCCUG 20 6343

HSV2-UL48-1009 + AGCAUAAAGUACCCAGAGGA 20 6344

HSV2-UL48-369 + CAGCAGCAUAAAGUACCCAG 20 5851

HSV2-UL48-1011 + UCAGCAGCAUAAAGUACCCA 20 6345

HSV2-UL48-1012 + GAUCAGCAGCAUAAAGUACC 20 6346

HSV2-UL48-1013 + GAGUACGAGUCCAACUUGGC 20 6347

HSV2-UL48-1014 + CGAGGUCGCGACGCUGGAGU 20 6348

HSV2-UL48-366 + GCCCUCCGAGGUCGCGACGC 20 5848

HSV2-UL48-1016 + CGCCCUCCGAGGUCGCGACG 20 6349

HSV2-UL48-1017 + CCGCAUGACGGACUCGCCCU 20 6350 HSV2-UL48-1018 + UAUACGCGUGCUCCCGCAUG 20 6351

HSV2-UL48-1019 + CGUCAUCGUCGUCCGGGAGG 20 6352

HSV2-UL48-359 + CAGGAGCGUCAUCGUCGUCC 20 5841

HSV2-UL48-358 + GCAGGAGCGUCAUCGUCGUC 20 5840

HSV2-UL48-1022 + CGCAGGAGCGUCAUCGUCGU 20 6353

HSV2-UL48-357 + GCCACCAGCCCGGCCUCCGC 20 5839

HSV2-UL48-1024 + CGCCACCAGCCCGGCCUCCG 20 6354

HSV2-UL48-1025 + CGGGGGCGUUGUCCCGCGGA 20 6355

HSV2-UL48-1026 + UGCGGGGGCGUUGUCCCGCG 20 6356

HSV2-UL48-354 + UCUGCGGGGGCGUUGUCCCG 20 5836

HSV2-UL48-1028 + GUCUGCGGGGGCGUUGUCCC 20 6357

HSV2-UL48-351 + GGCGGUGGUGGACAGUCUGC 20 5833

HSV2-UL48-350 + GGGCGGUGGUGGACAGUCUG 20 5832

HSV2-UL48-1031 + GGGGCGGUGGUGGACAGUCU 20 6358

HSV2-UL48-1032 + CGUCGGUAAUGGGGGCGGUG 20 6359

HSV2-UL48-344 + CCCAGGCUGACGUCGGUAAU 20 5826

HSV2-UL48-343 + UCCCAGGCUGACGUCGGUAA 20 5825

HSV2-UL48-1035 + CUCCCAGGCUGACGUCGGUA 20 6360

HSV2-UL48-340 + CCACCUCCUCGCCGUCCAGG 20 5822

HSV2-UL48-1037 + UCCACCUCCUCGCCGUCCAG 20 6361

HSV2-UL48-1038 + CGAAGUCGUCCAGGGCGUCG 20 6362

HSV2-UL48-1039 + AUCUCCAAGUCGAAGUCGUC 20 6363

HSV2-UL48-1040 + CGUCCCCCAGCAUCUCCAAG 20 6364

HSV2-UL48-332 + CGUGGGUCAUUCCCGGGGAG 20 5814

HSV2-UL48-331 + UCGUGGGUCAUUCCCGGGGA 20 5813

HSV2-UL48-330 + GUCGUGGGUCAUUCCCGGGG 20 5812

HSV2-UL48-1044 + GGUCGUGGGUCAUUCCCGGG 20 6365

HSV2-UL48-329 + GGGGUCGUGGGUCAUUCCCG 20 5811

HSV2-UL48-328 + CGGGGUCGUGGGUCAUUCCC 20 5810

HSV2-UL48-327 + ACGGGGUCGUGGGUCAUUCC 20 5809

HSV2-UL48-1048 + GACGGGGUCGUGGGUCAUUC 20 6366

HSV2-UL48-1049 + GCCCCAUACGAGACGGGGUC 20 6367

HSV2-UL48-322 + GUCCAAAGCCCCAUACGAGA 20 5804

HSV2-UL48-1051 + CGUCCAAAGCCCCAUACGAG 20 6368

HSV2-UL48-1052 + GUCCACGUCCAAAGCCCCAU 20 6369

HSV2-UL48-1053 + CAGGUACCGGUACAGGG 17 6370

HSV2-UL48-1054 + GCGCGCAGGUACCGGUA 17 6371

HSV2-UL48-1055 + GCCUGGCGGUGUAGCUG 17 6372

HSV2-UL48-1056 + AGGUAUAGAUGCAGAAA 17 6373

HSV2-UL48-1057 + AGAAAGAGGUAUAGAUG 17 6374

HSV2-UL48-1058 + GGAUCUCGCGGCUCAGA 17 6375

HSV2-UL48-1059 + CAUAGGAUCUCGCGGCU 17 6376

HSV2-UL48-1060 + UCGGCGUACGCGGCCCA 17 6377

HSV2-UL48-1061 + AGAGGCCGUCGAACAGA 17 6378

HSV2-UL48-1062 + GGUCGCAGCAGAGGCCG 17 6379

HSV2-UL48-1063 + AGCUCUCCAGGUCGCAG 17 6380 HSV2-UL48-1064 + UUGAUAAACAUCAGGGG 17 6381

HSV2-UL48-588 + AUCCGUUGAUAAACAUC 17 6070

HSV2-UL48-1066 + GAUCCGUUGAUAAACAU 17 6382

HSV2-UL48-1067 + CGGGAACUCCCCGCACG 17 6383

HSV2-UL48-586 + AGUCGCCGGGCCUCCAC 17 6068

HSV2-UL48-585 + CAGUCGCCGGGCCUCCA 17 6067

HSV2-UL48-1070 + GCAGUCGCCGGGCCUCC 17 6384

HSV2-UL48-1071 + UUUAGCUCCCGCAGUCG 17 6385

HSV2-UL48-1072 + GGGAGGUUCAGGUGCUC 17 6386

HSV2-UL48-579 + CCGCACUUCGCACCAGC 17 6061

HSV2-UL48-578 + GCCGCACUUCGCACCAG 17 6060

HSV2-UL48-1075 + CGCCGCACUUCGCACCA 17 6387

HSV2-UL48-1076 + GGGUCGUGAGGGGCGCC 17 6388

HSV2-UL48-573 + GGACGGGCGGGGUCGUG 17 6055

HSV2-UL48-1078 + AGGACGGGCGGGGUCGU 17 6389

HSV2-UL48-1079 + GCAGGACGGGCGGGGUC 17 6390

HSV2-UL48-570 + UUGCCCUGCAGGACGGG 17 6052

HSV2-UL48-1081 + GUUGCCCUGCAGGACGG 17 6391

HSV2-UL48-1082 + CCUGGUUGCCCUGCAGG 17 6392

HSV2-UL48-1083 + CGGGCCUGGUUGCCCUG 17 6393

HSV2-UL48-1084 + AUAAAGUACCCAGAGGA 17 6394

HSV2-UL48-563 + CAGCAUAAAGUACCCAG 17 6045

HSV2-UL48-1086 + GCAGCAUAAAGUACCCA 17 6395

HSV2-UL48-1087 + CAGCAGCAUAAAGUACC 17 6396

HSV2-UL48-1088 + UACGAGUCCAACUUGGC 17 6397

HSV2-UL48-1089 + GGUCGCGACGCUGGAGU 17 6398

HSV2-UL48-560 + CUCCGAGGUCGCGACGC 17 6042

HSV2-UL48-1091 + CCUCCGAGGUCGCGACG 17 6399

HSV2-UL48-1092 + CAUGACGGACUCGCCCU 17 6400

HSV2-UL48-1093 + ACGCGUGCUCCCGCAUG 17 6401

HSV2-UL48-1094 + CAUCGUCGUCCGGGAGG 17 6402

HSV2-UL48-553 + GAGCGUCAUCGUCGUCC 17 6035

HSV2-UL48-552 + GGAGCGUCAUCGUCGUC 17 6034

HSV2-UL48-1097 + AGGAGCGUCAUCGUCGU 17 6403

HSV2-UL48-551 + ACCAGCCCGGCCUCCGC 17 6033

HSV2-UL48-1099 + CACCAGCCCGGCCUCCG 17 6404

HSV2-UL48-1100 + GGGCGUUGUCCCGCGGA 17 6405

HSV2-UL48-1101 + GGGGGCGUUGUCCCGCG 17 6406

HSV2-UL48-548 + GCGGGGGCGUUGUCCCG 17 6030

HSV2-UL48-1103 + UGCGGGGGCGUUGUCCC 17 6407

HSV2-UL48-545 + GGUGGUGGACAGUCUGC 17 6027

HSV2-UL48-544 + CGGUGGUGGACAGUCUG 17 6026

HSV2-UL48-1106 + GCGGUGGUGGACAGUCU 17 6408

HSV2-UL48-1107 + CGGUAAUGGGGGCGGUG 17 6409

HSV2-UL48-538 + AGGCUGACGUCGGUAAU 17 6020

HSV2-UL48-537 + CAGGCUGACGUCGGUAA 17 6019 HSV2-UL48-1110 + CCAGGCUGACGUCGGUA 17 6410

HSV2-UL48-534 + CCUCCUCGCCGUCCAGG 17 6016

HSV2-UL48-1112 + ACCUCCUCGCCGUCCAG 17 6411

HSV2-UL48-1113 + AGUCGUCCAGGGCGUCG 17 6412

HSV2-UL48-1114 + UCCAAGUCGAAGUCGUC 17 6413

HSV2-UL48-1115 + CCCCCAGCAUCUCCAAG 17 6414

HSV2-UL48-526 + GGGUCAUUCCCGGGGAG 17 6008

HSV2-UL48-525 + UGGGUCAUUCCCGGGGA 17 6007

HSV2-UL48-524 + GUGGGUCAUUCCCGGGG 17 6006

HSV2-UL48-1119 + CGUGGGUCAUUCCCGGG 17 6415

HSV2-UL48-523 + GUCGUGGGUCAUUCCCG 17 6005

HSV2-UL48-522 + GGUCGUGGGUCAUUCCC 17 6004

HSV2-UL48-521 + GGGUCGUGGGUCAUUCC 17 6003

HSV2-UL48-1123 + GGGGUCGUGGGUCAUUC 17 6416

HSV2-UL48-1124 + CCAUACGAGACGGGGUC 17 6417

HSV2-UL48-516 + CAAAGCCCCAUACGAGA 17 5998

HSV2-UL48-1126 + CCAAAGCCCCAUACGAG 17 6418

HSV2-UL48-1127 + CACGUCCAAAGCCCCAU 17 6419

Table 3F provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence, but downstream of the first 500bp of the UL48 gene. In an embodiment, the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks. In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands and that are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 3F

Table 4A provides exemplary targeting domains for knocking out the UL54 gene selected according to first tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL54 gene and orthogonality against the human genome. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4A

HSV-UL54-24 + CGCCGGGGCCGUAUCGACCC 20 6447

HSV-UL54-25 + GGCCGUAUCGACCCCGGCCC 20 6448

HSV-UL54-26 + CGUAUCGACCCCGGCCCCGG 20 6449

HSV-UL54-27 + GGAUGCGGUGCCUUGGUCGA 20 6450

HSV-UL54-28 + GAUGCGGUGCCUUGGUCGAC 20 6451

HSV-UL54-29 + AUGCGGUGCCUUGGUCGACG 20 6452

HSV-UL54-30 + UGCGGUGCCUUGGUCGACGG 20 6453

HSV-UL54-31 + GUGCCUUGGUCGACGGGGGU 20 6454

HSV-UL54-32 + GGUCGACGGGGGUUGGAUGC 20 6455

HSV-UL54-33 + CGGGCCACCUUCCCCCCGUG 20 6456

HSV-UL54-34 + UCCCCCCGUGCGGUUCCCGG 20 6457

HSV-UL54-35 + CCCCCCGUGCGGUUCCCGGG 20 6458

HSV-UL54-36 + AAGCCG ACCG CCU GG U CCCG 20 6459

HSV-UL54-37 + CCCGAGGCGCGACCACACGC 20 6460

HSV-UL54-38 + GAGGCGCGACCACACGCCGG 20 6461

HSV-UL54-39 + CGACCACACGCCGGUGGUCG 20 6462

HSV-UL54-40 + GACCACACGCCGGUGGUCGC 20 6463

HSV-UL54-41 + CGGGCGAGGCGUCGAGGCUU 20 6464

HSV-UL54-42 + GGCGAGGCGUCGAGGCUUCG 20 6465

HSV-UL54-43 + GCGAGGCGUCGAGGCUUCGG 20 6466

HSV-UL54-44 + UCGGGGGUGCCGGCGUCCUC 20 6467

HSV-UL54-45 + GGGCGGGCCGGGGGACCUUU 20 6468

HSV-UL54-46 + UUUGGGAAUCGCCGCGUCGA 20 6469

HSV-UL54-47 + UUCCAUGUCCUCGUCCGACG 20 6470

HSV-UL54-48 + CUCCCCGCUGCUGUCGGACU 20 6471

HSV-UL54-49 + CCCCGCUGCUGUCGGACUCG 20 6472

HSV-UL54-50 + GUCCUCCUCGAGCUCGCUGU 20 6473

HSV-UL54-51 + CCUCGAGCUCGCUGUCGGAC 20 6474

HSV-UL54-52 + UGUCGGACAGGUCCAAUCCU 20 6475

HSV-UL54-53 - GAUAUGCUAAUCGACCU 17 6476

HSV-UL54-54 - GCUAAUCGACCUAGGAU 17 6477

HSV-UL54-55 - GUCCGACAGCGAGCUCG 17 6478

HSV-UL54-56 - CGACAGCGAGCUCGAGG 17 6479

HSV-UL54-57 - GCUCGAGGAGGACGCUC 17 6480

HSV-UL54-58 - CUCGUCGGACGAGGACA 17 6481

HSV-UL54-59 - ACCCCUGCGGAGACGGA 17 6482

HSV-UL54-60 - GGCGGAGGCCAUCGACG 17 6483

HSV-UL54-61 - GACGCGGCGAUUCCCAA 17 6484

HSV-UL54-62 - AGCCUCGACGCCUCGCC 17 6485

HSV-UL54-63 - CCGCCACCCGCGACCAC 17 6486

HSV-UL54-64 - CCGCGACCACCGGCGUG 17 6487

HSV-UL54-65 - GCGUGUGGUCGCGCCUC 17 6488

HSV-UL54-66 - GGUCGCGCCUCGGGACC 17 6489

HSV-UL54-67 - CGCGCCUCGGGACCAGG 17 6490

HSV-UL54-68 - UCCCCCCGGGAACCGCA 17 6491

HSV-UL54-69 - CCCCCGGGAACCGCACG 17 6492 HSV-UL54-70 - CCCCGGGAACCGCACGG 17 6493

HSV-UL54-71 - CCCGGGAACCGCACGGG 17 6494

HSV-UL54-72 - CGCAUCCCCGAGGCGGG 17 6495

HSV-UL54-73 - CGGGGCCGGGGUCGAUA 17 6496

HSV-UL54-74 - CGGGGUCGAUACGGCCC 17 6497

HSV-UL54-75 + CCGGCGGGGUUUUGGUG 17 6498

HSV-UL54-76 + CGGGGCCGUAUCGACCC 17 6499

HSV-UL54-77 + CGUAUCGACCCCGGCCC 17 6500

HSV-UL54-78 + AUCGACCCCGGCCCCGG 17 6501

HSV-UL54-79 + UGCGGUGCCUUGGUCGA 17 6502

HSV-UL54-80 + GCGGUGCCUUGGUCGAC 17 6503

HSV-UL54-81 + CGGUGCCUUGGUCGACG 17 6504

HSV-UL54-82 + GGUGCCUUGGUCGACGG 17 6505

HSV-UL54-83 + CCUUGGUCGACGGGGGU 17 6506

HSV-UL54-84 + CGACGGGGGUUGGAUGC 17 6507

HSV-UL54-85 + GCCACCU U CCCCCCG U G 17 6508

HSV-UL54-86 + CCCCGUGCGGU UCCCGG 17 6509

HSV-UL54-87 + CCCGUGCGGU UCCCGGG 17 6510

HSV-UL54-88 + CCGACCGCCUGGUCCCG 17 6511

HSV-UL54-89 + GAGGCGCGACCACACGC 17 6512

HSV-UL54-90 + GCGCGACCACACGCCGG 17 6513

HSV-UL54-91 + CCACACGCCGGUGGUCG 17 6514

HSV-UL54-92 + CACACGCCGGUGGUCGC 17 6515

HSV-UL54-93 + GCGAGGCGUCGAGGCUU 17 6516

HSV-UL54-94 + GAGGCGUCGAGGCUUCG 17 6517

HSV-UL54-95 + AGGCGUCGAGGCUUCGG 17 6518

HSV-UL54-96 + GGGGUGCCGGCGUCCUC 17 6519

HSV-UL54-97 + CGGGCCGGGGGACCUUU 17 6520

HSV-UL54-98 + GGGAAUCGCCGCGUCGA 17 6521

HSV-UL54-99 + CAUGUCCUCGUCCGACG 17 6522

HSV-UL54-100 + CCCGCUGCUGUCGGACU 17 6523

HSV-UL54-101 + CGCUGCUGUCGGACUCG 17 6524

HSV-UL54-102 + CUCCUCGAGCUCGCUGU 17 6525

HSV-UL54-103 + CGAGCUCGCUGUCGGAC 17 6526

HSV-UL54-104 + CGGACAGGUCCAAUCCU 17 6527

Table 4B provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains are selected based on location within the first 500bp of the coding sequence of the UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4B

HSV-UL54-138 - AGGCACCGCAUCCCCGAGGC 20 6561

HSV-UL54-139 - CAUCCCCGAGGCGGGCGGCG 20 6562

HSV-UL54-140 - GCGGGCGGCGAGGUCGCCGC 20 6563

HSV-UL54-141 - CGGGCGGCGAGGUCGCCGCC 20 6564

HSV-UL54-142 - GGGCGGCGAGGUCGCCGCCG 20 6565

HSV-UL54-143 - GGCGAGGUCGCCGCCGGGGC 20 6566

HSV-UL54-144 - GCGAGGUCGCCGCCGGGGCC 20 6567

HSV-UL54-145 - CGAGGUCGCCGCCGGGGCCG 20 6568

HSV-UL54-146 - CGGGGUCGAUACGGCCCCGG 20 6569

HSV-UL54-147 - ACUCCACACCAAAACCCCGC 20 6570

HSV-UL54-148 + GCGGGGUUUUGGUGUGGAGU 20 6571

HSV-UL54-149 + UGGUGUGGAGUCGGCGCCGC 20 6572

HSV-UL54-150 + GGUGUGGAGUCGGCGCCGCC 20 6573

HSV-UL54-151 + GUGUGGAGUCGGCGCCGCCG 20 6574

HSV-UL54-152 + GGCGACCUCGCCGCCCGCCU 20 6575

HSV-UL54-153 + GCGACCUCGCCGCCCGCCUC 20 6576

HSV-UL54-154 + CGACCUCGCCGCCCGCCUCG 20 6577

HSV-UL54-155 + CGCCGCCCGCCUCGGGGAUG 20 6578

HSV-UL54-156 + GCCUCGGGGAUGCGGUGCCU 20 6579

HSV-UL54-157 + UGGUCGACGGGGGUUGGAUG 20 6580

HSV-UL54-158 + CCUUCCCCCCGUGCGGUUCC 20 6581

HSV-UL54-159 + CUUCCCCCCGUGCGGUUCCC 20 6582

HSV-UL54-160 + UUCCCCCCGUGCGGUUCCCG 20 6583

HSV-UL54-161 + CCGGGGGGAAGCCGACCGCC 20 6584

HSV-UL54-162 + CACACGCCGGUGGUCGCGGG 20 6585

HSV-UL54-163 + ACGCCGGUGGUCGCGGGUGG 20 6586

HSV-UL54-164 + GGUCGCGGGUGGCGGAUCGU 20 6587

HSV-UL54-165 + UCGGCUCCCCGCCGCGCUGC 20 6588

HSV-UL54-166 + CGGCUCCCCGCCGCGCUGCC 20 6589

HSV-UL54-167 + CCCCGCCGCGCUGCCGGGCG 20 6590

HSV-UL54-168 + CGCUGCCGGGCGAGGCGUCG 20 6591

HSV-UL54-169 + GGGCGAGGCGUCGAGGCUUC 20 6592

HSV-UL54-170 + CGUCGAGGCUUCGGGGGUGC 20 6593

HSV-UL54-171 + UUCGGGGGUGCCGGCGUCCU 20 6594

HSV-UL54-172 + CGGGGGUGCCGGCGUCCUCG 20 6595

HSV-UL54-173 + GGGUGCCGGCGUCCUCGGGG 20 6596

HSV-UL54-174 + GGUGCCGGCGUCCUCGGGGC 20 6597

HSV-UL54-175 + CCGGCGUCCUCGGGGCGGGC 20 6598

HSV-UL54-176 + CGGCGUCCUCGGGGCGGGCC 20 6599

HSV-UL54-177 + GGCGUCCUCGGGGCGGGCCG 20 6600

HSV-UL54-178 + GCGUCCUCGGGGCGGGCCGG 20 6601

HSV-UL54-179 + GGGGCGGGCCGGGGGACCUU 20 6602

HSV-UL54-180 + CUCCGCCCCUCCGUCUCCGC 20 6603

HSV-UL54-181 + U CCG CCCCU CCG U CU CCG CA 20 6604

HSV-UL54-182 + CCGCCCCUCCGUCUCCGCAG 20 6605

HSV-UL54-183 + GGAACACUCCCCGCUGCUGU 20 6606 HSV-UL54-184 + UCCCCGCUGCUGUCGGACUC 20 6607

HSV-UL54-185 + UGUCGGACUCGGGGUCGUCG 20 6608

HSV-UL54-186 + CGGACUCGGGGUCGUCGCGG 20 6609

HSV-UL54-187 + GUCGAUUAGCAUAUCAAUGU 20 6610

HSV-UL54-188 - AGGAGGACGCUCUGGAG 17 6611

HSV-UL54-189 - GGAGGACGCUCUGGAGC 17 6612

HSV-UL54-190 - CGCUCUGGAGCGGGACG 17 6613

HSV-UL54-191 - UCUGGAGCGGGACGAGG 17 6614

HSV-UL54-192 - CUGGAGCGGGACGAGGA 17 6615

HSV-UL54-193 - CCCGAGUCCGACAGCAG 17 6616

HSV-UL54-194 - CCGAGUCCGACAGCAGC 17 6617

HSV-UL54-195 - CGAGUCCGACAGCAGCG 17 6618

HSV-UL54-196 - CGGGGAGUGUUCCUCGU 17 6619

HSV-UL54-197 - GUGUUCCUCGUCGGACG 17 6620

HSV-UL54-198 - GACAUGGAAGACCCCUG 17 6621

HSV-UL54-199 - GAAGACCCCUGCGGAGA 17 6622

HSV-UL54-200 - GACCCCUGCGGAGACGG 17 6623

HSV-UL54-201 - CCCCUGCGGAGACGGAG 17 6624

HSV-UL54-202 - CUGCGGAGACGGAGGGG 17 6625

HSV-UL54-203 - CGGAGACGGAGGGGCGG 17 6626

HSV-UL54-204 - GAUUCCCAAAGGUCCCC 17 6627

HSV-UL54-205 - UCCCCCGGCCCGCCCCG 17 6628

HSV-UL54-206 - GCCCGCCCCGAGGACGC 17 6629

HSV-UL54-207 - CGCCUCGCCCGGCAGCG 17 6630

HSV-UL54-208 - CUCGCCCGGCAGCGCGG 17 6631

HSV-UL54-209 - UCGCCCGGCAGCGCGGC 17 6632

HSV-UL54-210 - CGCCCGGCAGCGCGGCG 17 6633

HSV-UL54-211 - GGCGUGUGGUCGCGCCU 17 6634

HSV-UL54-212 - CCUCGGGACCAGGCGGU 17 6635

HSV-UL54-213 - GGCGGUCGGCUUCCCCC 17 6636

HSV-UL54-214 - GCGGUCGGCUUCCCCCC 17 6637

HSV-UL54-215 - CCCCCCGGGAACCGCAC 17 6638

HSV-UL54-216 - GGAACCGCACGGGGGGA 17 6639

HSV-UL54-217 - ACCGCACGGGGGGAAGG 17 6640

HSV-UL54-218 - CCAACCCCCG UCG ACCA 17 6641

HSV-UL54-219 - AAGGCACCGCAUCCCCG 17 6642

HSV-UL54-220 - GCACCGCAUCCCCGAGG 17 6643

HSV-UL54-221 - CACCGCAUCCCCGAGGC 17 6644

HSV-UL54-222 - CCCCGAGGCGGGCGGCG 17 6645

HSV-UL54-223 - GGCGGCGAGGUCGCCGC 17 6646

HSV-UL54-224 - GCGGCGAGGUCGCCGCC 17 6647

HSV-UL54-225 - CGGCGAGGUCGCCGCCG 17 6648

HSV-UL54-226 - GAGGUCGCCGCCGGGGC 17 6649

HSV-UL54-227 - AGGUCGCCGCCGGGGCC 17 6650

HSV-UL54-228 - GGUCGCCGCCGGGGCCG 17 6651

HSV-UL54-229 - GGUCGAUACGGCCCCGG 17 6652 HSV-UL54-230 - CCACACCAAAACCCCGC 17 6653

HSV-UL54-231 + GGGUUUUGGUGUGGAGU 17 6654

HSV-UL54-232 + UGUGGAGUCGGCGCCGC 17 6655

HSV-UL54-233 + GUGGAGUCGGCGCCGCC 17 6656

HSV-UL54-234 + UGGAGUCGGCGCCGCCG 17 6657

HSV-UL54-235 + GACCUCGCCGCCCGCCU 17 6658

HSV-UL54-236 + ACCUCGCCGCCCGCCUC 17 6659

HSV-UL54-237 + CCUCGCCGCCCGCCUCG 17 6660

HSV-UL54-238 + CGCCCGCCUCGGGGAUG 17 6661

HSV-UL54-239 + UCGGGGAUGCGGUGCCU 17 6662

HSV-UL54-240 + UCGACGGGGGUUGGAUG 17 6663

HSV-UL54-241 + UCCCCCCGUGCGGUUCC 17 6664

HSV-UL54-242 + CCCCCCGUGCGGUUCCC 17 6665

HSV-UL54-243 + CCCCCGUGCGGUUCCCG 17 6666

HSV-UL54-244 + GGGGGAAGCCGACCGCC 17 6667

HSV-UL54-245 + ACGCCGGUGGUCGCGGG 17 6668

HSV-UL54-246 + CCGGUGGUCGCGGGUGG 17 6669

HSV-UL54-247 + CGCGGGUGGCGGAUCGU 17 6670

HSV-UL54-248 + GCUCCCCGCCGCGCUGC 17 6671

HSV-UL54-249 + CUCCCCGCCGCGCUGCC 17 6672

HSV-UL54-250 + CGCCGCGCUGCCGGGCG 17 6673

HSV-UL54-251 + UGCCGGGCGAGGCGUCG 17 6674

HSV-UL54-252 + CGAGGCGUCGAGGCUUC 17 6675

HSV-UL54-253 + CGAGGCUUCGGGGGUGC 17 6676

HSV-UL54-254 + GGGGGUGCCGGCGUCCU 17 6677

HSV-UL54-255 + GGGUGCCGGCGUCCUCG 17 6678

HSV-UL54-256 + UGCCGGCGUCCUCGGGG 17 6679

HSV-UL54-257 + GCCGGCGUCCUCGGGGC 17 6680

HSV-UL54-258 + GCGUCCUCGGGGCGGGC 17 6681

HSV-UL54-259 + CGUCCUCGGGGCGGGCC 17 6682

HSV-UL54-260 + GUCCUCGGGGCGGGCCG 17 6683

HSV-UL54-261 + UCCUCGGGGCGGGCCGG 17 6684

HSV-UL54-262 + GCGGGCCGGGGGACCUU 17 6685

HSV-UL54-263 + CGCCCCUCCGUCUCCGC 17 6686

HSV-UL54-264 + GCCCCUCCGUCUCCGCA 17 6687

HSV-UL54-265 + CCCCUCCGUCUCCGCAG 17 6688

HSV-UL54-266 + ACACU CCCCG CU GCU G U 17 6689

HSV-UL54-267 + CCGCUGCUGUCGGACUC 17 6690

HSV-UL54-268 + CGGACUCGGGGUCGUCG 17 6691

HSV-UL54-269 + ACUCGGGGUCGUCGCGG 17 6692

HSV-UL54-270 + GAUUAGCAUAUCAAUGU 17 6693

Table 4C provides exemplary targeting domains for knocking out the UL54 gene selected according to the third tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of the UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. pyogenes Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4C

HSV-UL54-298 - ACACCAU CG ACCCCG CCG U U 20 6721

HSV-UL54-299 - CACCAUCGACCCCGCCGUUC 20 6722

HSV-UL54-300 - CAUCGACCCCGCCGUUCGGG 20 6723

HSV-UL54-301 - GCGAUCCAUAUCCGAGCGCG 20 6724

HSV-UL54-302 - AUCCAUAUCCGAGCGCGCGG 20 6725

HSV-UL54-303 - GAGCGCAUCAGCGAAAGCUU 20 6726

HSV-UL54-304 - AAGCUUUGGACGCAGUGCCC 20 6727

HSV-UL54-305 - CUGGUCAUGCAAGACCCCUU 20 6728

HSV-UL54-306 - GUCAUGCAAGACCCCUUUGG 20 6729

HSV-UL54-307 - UCAUGCAAGACCCCUUUGGC 20 6730

HSV-UL54-308 - UUCCCGCCGCGAACAGCCCC 20 6731

HSV-UL54-309 - U CCCG CCGCG AACAG CCCCU 20 6732

HSV-UL54-310 - CAGCCCCUGGGCUCCCGUGC 20 6733

HSV-UL54-311 - UCCCGUGCUGGCCACCCAAG 20 6734

HSV-UL54-312 - CCCGUGCUGGCCACCCAAGC 20 6735

HSV-UL54-313 - CCGUGCUGGCCACCCAAGCG 20 6736

HSV-UL54-314 - CGUGCUGGCCACCCAAGCGG 20 6737

HSV-UL54-315 - GUGCUGGCCACCCAAGCGGG 20 6738

HSV-UL54-316 - UGCUGGCCACCCAAGCGGGG 20 6739

HSV-UL54-317 - GGUUUGACGCCGAGACCCGU 20 6740

HSV-UL54-318 - GUUUGACGCCGAGACCCGUC 20 6741

HSV-UL54-319 - CCGAGACCCGUCGGGUUUCC 20 6742

HSV-UL54-320 - CGAGACCCGUCGGGUUUCCU 20 6743

HSV-UL54-321 - UCGGGUUUCCUGGGAAACCC 20 6744

HSV-UL54-322 - UGGGAAACCCUGGUCGCUCA 20 6745

HSV-UL54-323 - GCACAUUCGCAGCCAACCCG 20 6746

HSV-UL54-324 - CACAUUCGCAGCCAACCCGC 20 6747

HSV-UL54-325 - GCGGGCCGCGUCGACAGCCA 20 6748

HSV-UL54-326 - GCGCGACUGCGUGCUGCGCC 20 6749

HSV-UL54-327 - GCGCCAGGAAAAUCUCAUCG 20 6750

HSV-UL54-328 - GGAAAAUCUCAUCGAGGCCC 20 6751

HSV-UL54-329 - CAUCGAGGCCCUGGCGUCCG 20 6752

HSV-UL54-330 - GGCGUCCGCGGAUGAGACGC 20 6753

HSV-UL54-331 - CCGCGGAUGAGACGCUGGCG 20 6754

HSV-UL54-332 - CAAUCUGCCGCUCCGCCCCC 20 6755

HSV-UL54-333 - CGCCCCCAGGACCCUAUCAU 20 6756

HSV-UL54-334 - CCAGGACCCUAUCAUCGGAA 20 6757

HSV-UL54-335 - GGACCCUAUCAUCGGAACGG 20 6758

HSV-UL54-336 - CGGAACGGCGGCCGCCGUGC 20 6759

HSV-UL54-337 - CUUUCUGCAGUGCUACCUGA 20 6760

HSV-UL54-338 - CAGUGCUACCUGAAGGCCCG 20 6761

HSV-UL54-339 - CUGAAGGCCCGAGGCCUGUG 20 6762

HSV-UL54-340 - UGAAGGCCCGAGGCCUGUGC 20 6763

HSV-UL54-341 - GGCCCGAGGCCUGUGCGGGC 20 6764

HSV-UL54-342 - GGCUGGACGACCUGUGCUCG 20 6765

HSV-UL54-343 - GUGCUCGCGGCGACGCCUGU 20 6766 HSV-UL54-344 - GCGACGCCUGUCGGACAUUA 20 6767

HSV-UL54-345 - GGAUAUUGCCUCCUUUGUGU 20 6768

HSV-UL54-346 - CUCCUUUGUGUUGGUCAUCC 20 6769

HSV-UL54-347 - CU CG CCAACCGCG U CG AG CG 20 6770

HSV-UL54-348 - CCGCGUCGAGCGCGGCGUGU 20 6771

HSV-UL54-349 - GGAGAUCGACUACACGACCG 20 6772

HSV-UL54-350 - GAGAUCGACUACACGACCGU 20 6773

HSV-UL54-351 - AGAUCGACUACACGACCGUG 20 6774

HSV-UL54-352 - GAUCGACUACACGACCGUGG 20 6775

HSV-UL54-353 - GACUACACGACCGUGGGGGU 20 6776

HSV-UL54-354 - ACUACACGACCGUGGGGGUU 20 6777

HSV-UL54-355 - CUACACGACCGUGGGGGUUG 20 6778

HSV-UL54-356 - ACGACCGUGGGGGUUGGGGC 20 6779

HSV-UL54-357 - GACGAUGCACUUUUACAUCC 20 6780

HSV-UL54-358 - ACGAUGCACUUUUACAUCCC 20 6781

HSV-UL54-359 - CGAUGCACUUUUACAUCCCG 20 6782

HSV-UL54-360 - GAUGCACUUUUACAUCCCGG 20 6783

HSV-UL54-361 - UUACAUCCCGGGGGCCUGCA 20 6784

HSV-UL54-362 - CAUCCCGGGGGCCUGCAUGG 20 6785

HSV-UL54-363 - AUCCCGGGGGCCUGCAUGGC 20 6786

HSV-UL54-364 - GGCGGGUCUCAUUGAAAUAC 20 6787

HSV-UL54-365 - AAUACUGGACACGCACCGCC 20 6788

HSV-UL54-366 - CAGUCGCGUGUGCGAGCUGA 20 6789

HSV-UL54-367 - AUCGCCCCCUUAUAUGUGCA 20 6790

HSV-UL54-368 + UGCACAUAUAAGGGGGCGAU 20 6791

HSV-UL54-369 + UCAGCUCGCACACGCGACUG 20 6792

HSV-UL54-370 + GGCGGUGCGUGUCCAGUAUU 20 6793

HSV-UL54-371 + GUAUUUCAAUGAGACCCGCC 20 6794

HSV-UL54-372 + GCCAUGCAGGCCCCCGGGAU 20 6795

HSV-UL54-373 + CCAUGCAGGCCCCCGGGAUG 20 6796

HSV-UL54-374 + UGCAGGCCCCCGGGAUGUAA 20 6797

HSV-UL54-375 + CCGGGAUGUAAAAGUGCAUC 20 6798

HSV-UL54-376 + CGGGAUGUAAAAGUGCAUCG 20 6799

HSV-UL54-377 + GGGAUGUAAAAGUGCAUCGU 20 6800

HSV-UL54-378 + GGAUGUAAAAGUGCAUCGUC 20 6801

HSV-UL54-379 + G CCCCAACCCCCACG G U CG U 20 6802

HSV-UL54-380 + CAACCCCCACGGUCGUGUAG 20 6803

HSV-UL54-381 + AACCCCCACGGUCGUGUAGU 20 6804

HSV-UL54-382 + ACCCCCACGGUCGUGUAGUC 20 6805

HSV-UL54-383 + CCACGGUCGUGUAGUCGAUC 20 6806

HSV-UL54-384 + CACGGUCGUGUAGUCGAUCU 20 6807

HSV-UL54-385 + ACGGUCGUGUAGUCGAUCUC 20 6808

HSV-UL54-386 + CGGUCGUGUAGUCGAUCUCC 20 6809

HSV-UL54-387 + ACACGCCGCGCUCGACGCGG 20 6810

HSV-UL54-388 + CGCUCGACGCGGUUGGCGAG 20 6811

HSV-UL54-389 + GGAUGACCAACACAAAGGAG 20 6812 HSV-UL54-390 + ACACAAAGGAGGCAAUAUCC 20 6813

HSV-UL54-391 + UAAUGUCCGACAGGCGUCGC 20 6814

HSV-UL54-392 + ACAGGCGUCGCCGCGAGCAC 20 6815

HSV-UL54-393 + CGAGCACAGGUCGUCCAGCC 20 6816

HSV-UL54-394 + GCCCGCACAGGCCUCGGGCC 20 6817

HSV-UL54-395 + GCACAGGCCUCGGGCCUUCA 20 6818

HSV-UL54-396 + CACAGGCCUCGGGCCUUCAG 20 6819

HSV-UL54-397 + CGGGCCUUCAGGUAGCACUG 20 6820

HSV-UL54-398 + UCAGGUAGCACUGCAGAAAG 20 6821

HSV-UL54-399 + GCACGGCGGCCGCCGUUCCG 20 6822

HSV-UL54-400 + CCGU UCCGAUGAUAGGGUCC 20 6823

HSV-UL54-401 + UUCCGAUGAUAGGGUCCUGG 20 6824

HSV-UL54-402 + AUGAUAGGGUCCUGGGGGCG 20 6825

HSV-UL54-403 + GGGGGCGGAGCGGCAGAUUG 20 6826

HSV-UL54-404 + CGCCAGCGUCUCAUCCGCGG 20 6827

HSV-UL54-405 + GCGUCUCAUCCGCGGACGCC 20 6828

HSV-UL54-406 + CGGACGCCAGGGCCUCGAUG 20 6829

HSV-UL54-407 + GGGCCUCGAUGAGAUUUUCC 20 6830

HSV-UL54-408 + CGAUGAGAUUUUCCUGGCGC 20 6831

HSV-UL54-409 + GGCGCAGCACGCAGUCGCGC 20 6832

HSV-UL54-410 + UGGCUGUCGACGCGGCCCGC 20 6833

HSV-UL54-411 + GCGGGUUGGCUGCGAAUGUG 20 6834

HSV-UL54-412 + CGGGUUGGCUGCGAAUGUGC 20 6835

HSV-UL54-413 + UGAGCGACCAGGGUUUCCCA 20 6836

HSV-UL54-414 + G GG U U U CCCAG G AAACCCG A 20 6837

HSV-UL54-415 + AGGAAACCCGACGGGUCUCG 20 6838

HSV-UL54-416 + GGAAACCCGACGGGUCUCGG 20 6839

HSV-UL54-417 + GACGGGUCUCGGCGUCAAAC 20 6840

HSV-UL54-418 + ACGGGUCUCGGCGUCAAACC 20 6841

HSV-UL54-419 + CCCCGCUUGGGUGGCCAGCA 20 6842

HSV-UL54-420 + CCCGCUUGGGUGGCCAGCAC 20 6843

HSV-UL54-421 + CCGCUUGGGUGGCCAGCACG 20 6844

HSV-UL54-422 + CGCUUGGGUGGCCAGCACGG 20 6845

HSV-UL54-423 + GCUUGGGUGGCCAGCACGGG 20 6846

HSV-UL54-424 + CUUGGGUGGCCAGCACGGGA 20 6847

HSV-UL54-425 + GCACGGGAGCCCAGGGGCUG 20 6848

HSV-UL54-426 + AGGGGCUGUUCGCGGCGGGA 20 6849

HSV-UL54-427 + GGGGCUGUUCGCGGCGGGAA 20 6850

HSV-UL54-428 + GCCAAAGGGGUCUUGCAUGA 20 6851

HSV-UL54-429 + CCAAAGGGGUCUUGCAUGAC 20 6852

HSV-UL54-430 + AAGGGGUCUUGCAUGACCAG 20 6853

HSV-UL54-431 + GGGCACUGCGUCCAAAGCUU 20 6854

HSV-UL54-432 + AAGCUUUCGCUGAUGCGCUC 20 6855

HSV-UL54-433 + CCGCGCGCUCGGAUAUGGAU 20 6856

HSV-UL54-434 + CGCGCUCGGAUAUGGAUCGC 20 6857

HSV-UL54-435 + CCCGAACGGCGGGGUCGAUG 20 6858 HSV-UL54-436 + GAACGGCGGGGUCGAUGGUG 20 6859

HSV-UL54-437 + AACGGCGGGGUCGAUGGUGU 20 6860

HSV-UL54-438 + UUCGCUCCGGGACCGGGGCG 20 6861

HSV-UL54-439 + GGACCGGGGCGCGGCCGUCC 20 6862

HSV-UL54-440 + GGGCGCGGCCGUCCGCGUGC 20 6863

HSV-UL54-441 + UCCGCGUGCGGGGGGGUCAG 20 6864

HSV-UL54-442 + CGUGCGGGGGGGUCAGGGAC 20 6865

HSV-UL54-443 + UCAGCGGAGGGGGGGCCUGG 20 6866

HSV-UL54-444 + GGCGCGUGCCUCGUGGCCGC 20 6867

HSV-UL54-445 + CGUGGCCGCGGGCCCCCGGA 20 6868

HSV-UL54-446 + CGGGCCCCCGGAGACGUCGA 20 6869

HSV-UL54-447 + CCGGAGACGUCGAGCUGCUC 20 6870

HSV-UL54-448 + CGGAGACGUCGAGCUGCUCC 20 6871

HSV-UL54-449 + GGAGACGUCGAGCUGCUCCC 20 6872

HSV-UL54-450 + GAGACGUCGAGCUGCUCCCC 20 6873

HSV-UL54-451 + CUCCGCGCCGCGCCUCGCCG 20 6874

HSV-UL54-452 + UCCGCGCCGCGCCUCGCCGU 20 6875

HSV-UL54-453 + CCGCGCCGCGCCUCGCCGUG 20 6876

HSV-UL54-454 + CGCCGCGCCUCGCCGUGGGU 20 6877

HSV-UL54-455 + CGCCUCGCCGUGGGUGGCGC 20 6878

HSV-UL54-456 + CGCCGUGGGUGGCGCCGGGG 20 6879

HSV-UL54-457 + UGGGUGGCGCCGGGGCCGUC 20 6880

HSV-UL54-458 + GGGCCGUCCGUCCGCGCCGA 20 6881

HSV-UL54-459 + UCCGUCCGCGCCGACGCGGG 20 6882

HSV-UL54-460 + UCCGCGCCGACGCGGGGUGG 20 6883

HSV-UL54-461 + CGCCGACGCGGGGUGGCGAC 20 6884

HSV-UL54-462 + ACGCGGGGUGGCGACCCCCU 20 6885

HSV-UL54-463 + CCUUGGUUGUGGGCGUUUCU 20 6886

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-467 - AAACG CCCACAACCAAG 17 6888

HSV-UL54-468 - AACG CCCACAACCAAG G 17 6889

HSV-UL54-469 - GGGUCGCCACCCCGCGU 17 6890

HSV-UL54-470 - GCCACCCCGCGUCGGCG 17 6891

HSV-UL54-471 - CCCCGCGUCGGCGCGGA 17 6892

HSV-UL54-472 - GCGUCGGCGCGGACGGA 17 6893

HSV-UL54-473 - GCGCGGACGGACGGCCC 17 6894

HSV-UL54-474 - GGCCCCGGCGCCACCCA 17 6895

HSV-UL54-475 - CGGCGCCACCCACGGCG 17 6896

HSV-UL54-476 - CCACCCACGGCGAGGCG 17 6897

HSV-UL54-477 - CACGGCGAGGCGCGGCG 17 6898

HSV-UL54-478 - GGCGAGGCGCGGCGCGG 17 6899

HSV-UL54-479 - GCGAGGCGCGGCGCGGA 17 6900

HSV-UL54-480 - CGAGGCGCGGCGCGGAG 17 6901

HSV-UL54-481 - GAGCAGCUCGACGUCUC 17 6902 HSV-UL54-482 - AGCAGCUCGACGUCUCC 17 6903

HSV-UL54-483 - GCAGCUCGACGUCUCCG 17 6904

HSV-UL54-484 - CAGCUCGACGUCUCCGG 17 6905

HSV-UL54-485 - ACGUCUCCGGGGGCCCG 17 6906

HSV-UL54-486 - GGGGGCCCGCGGCCACG 17 6907

HSV-UL54-487 - GCCACGAGGCACGCGCC 17 6908

HSV-UL54-488 - GGCCCCCCCUCCGCUGA 17 6909

HSV-UL54-489 - CCUGACCCCCCCGCACG 17 6910

HSV-UL54-490 - ACCCCCCCGCACGCGGA 17 6911

HSV-UL54-491 - CGCGGACGGCCGCGCCC 17 6912

HSV-UL54-492 - CGGCCGCGCCCCGGUCC 17 6913

HSV-UL54-493 - CCCGGUCCCGGAGCGAA 17 6914

HSV-UL54-494 - CCAUCGACCCCGCCGUU 17 6915

HSV-UL54-495 - CAUCGACCCCGCCGUUC 17 6916

HSV-UL54-496 - CGACCCCGCCGUUCGGG 17 6917

HSV-UL54-497 - AUCCAUAUCCGAGCGCG 17 6918

HSV-UL54-498 - CAUAUCCGAGCGCGCGG 17 6919

HSV-UL54-499 - CGCAUCAGCGAAAGCUU 17 6920

HSV-UL54-500 - CUUUGGACGCAGUGCCC 17 6921

HSV-UL54-501 - G U CAU GCAAG ACCCCU U 17 6922

HSV-UL54-502 - AUGCAAGACCCCUUUGG 17 6923

HSV-UL54-503 - UGCAAGACCCCUUUGGC 17 6924

HSV-UL54-504 - CCGCCGCGAACAGCCCC 17 6925

HSV-UL54-505 - CGCCGCGAACAGCCCCU 17 6926

HSV-UL54-506 - CCCCUGGGCUCCCGUGC 17 6927

HSV-UL54-507 - CGUGCUGGCCACCCAAG 17 6928

HSV-UL54-508 - GUGCUGGCCACCCAAGC 17 6929

HSV-UL54-509 - UGCUGGCCACCCAAGCG 17 6930

HSV-UL54-510 - GCUGGCCACCCAAGCGG 17 6931

HSV-UL54-511 - CUGGCCACCCAAGCGGG 17 6932

HSV-UL54-512 - UGGCCACCCAAGCGGGG 17 6933

HSV-UL54-513 - UUGACGCCGAGACCCGU 17 6934

HSV-UL54-514 - UGACGCCGAGACCCGUC 17 6935

HSV-UL54-515 - AGACCCGUCGGGUUUCC 17 6936

HSV-UL54-516 - GACCCGUCGGGUUUCCU 17 6937

HSV-UL54-517 - GGUUUCCUGGGAAACCC 17 6938

HSV-UL54-518 - GAAACCCUGGUCGCUCA 17 6939

HSV-UL54-519 - CAUUCGCAGCCAACCCG 17 6940

HSV-UL54-520 - AUUCGCAGCCAACCCGC 17 6941

HSV-UL54-521 - GGCCGCGUCGACAGCCA 17 6942

HSV-UL54-522 - CGACUGCGUGCUGCGCC 17 6943

HSV-UL54-523 - CCAG G AAAAU CU CAUCG 17 6944

HSV-UL54-524 - AAAU CU CAU CG AGG CCC 17 6945

HSV-UL54-525 - CGAGGCCCUGGCGUCCG 17 6946

HSV-UL54-526 - GUCCGCGGAUGAGACGC 17 6947

HSV-UL54-527 - CGGAUGAGACGCUGGCG 17 6948 HSV-UL54-528 - UCUGCCGCUCCGCCCCC 17 6949

HSV-UL54-529 - CCCCAGGACCCUAUCAU 17 6950

HSV-UL54-530 - GGACCCUAUCAUCGGAA 17 6951

HSV-UL54-531 - CCCUAUCAUCGGAACGG 17 6952

HSV-UL54-532 - AACGGCGGCCGCCGUGC 17 6953

HSV-UL54-533 - UCUGCAGUGCUACCUGA 17 6954

HSV-UL54-534 - UGCUACCUGAAGGCCCG 17 6955

HSV-UL54-535 - AAGGCCCGAGGCCUGUG 17 6956

HSV-UL54-536 - AGGCCCGAGGCCUGUGC 17 6957

HSV-UL54-537 - CCGAGGCCUGUGCGGGC 17 6958

HSV-UL54-538 - UGGACGACCUGUGCUCG 17 6959

HSV-UL54-539 - CUCGCGGCGACGCCUGU 17 6960

HSV-UL54-540 - ACGCCUGUCGGACAUUA 17 6961

HSV-UL54-541 - UAUUGCCUCCUUUGUGU 17 6962

HSV-UL54-542 - CUUUGUGUUGGUCAUCC 17 6963

HSV-UL54-543 - GCCAACCGCGUCGAGCG 17 6964

HSV-UL54-544 - CGUCGAGCGCGGCGUGU 17 6965

HSV-UL54-545 - GAUCGACUACACGACCG 17 6966

HSV-UL54-546 - AUCGACUACACGACCGU 17 6967

HSV-UL54-547 - UCGACUACACGACCGUG 17 6968

HSV-UL54-548 - CGACUACACGACCGUGG 17 6969

HSV-UL54-549 - UACACGACCGUGGGGGU 17 6970

HSV-UL54-550 - ACACGACCGUGGGGGUU 17 6971

HSV-UL54-551 - CACGACCGUGGGGGUUG 17 6972

HSV-UL54-552 - ACCGUGGGGGUUGGGGC 17 6973

HSV-UL54-553 - GAUGCACUUUUACAUCC 17 6974

HSV-UL54-554 - AUGCACUUUUACAUCCC 17 6975

HSV-UL54-555 - UGCACUUUUACAUCCCG 17 6976

HSV-UL54-556 - GCACUUUUACAUCCCGG 17 6977

HSV-UL54-557 - CAUCCCGGGGGCCUGCA 17 6978

HSV-UL54-558 - CCCGGGGGCCUGCAUGG 17 6979

HSV-UL54-559 - CCGGGGGCCUGCAUGGC 17 6980

HSV-UL54-560 - GGGUCUCAUUGAAAUAC 17 6981

HSV-UL54-561 - ACUGGACACGCACCGCC 17 6982

HSV-UL54-562 - UCGCGUGUGCGAGCUGA 17 6983

HSV-UL54-563 - GCCCCCUUAUAUGUGCA 17 6984

HSV-UL54-564 + UGCACAUAUAAGGGGGC 17 6985

HSV-UL54-565 + UCAGCUCGCACACGCGA 17 6986

HSV-UL54-566 + GGCGGUGCGUGUCCAGU 17 6987

HSV-UL54-567 + GUAUUUCAAUGAGACCC 17 6988

HSV-UL54-568 + GCCAUGCAGGCCCCCGG 17 6989

HSV-UL54-569 + CCAUGCAGGCCCCCGGG 17 6990

HSV-UL54-570 + UGCAGGCCCCCGGGAUG 17 6991

HSV-UL54-571 + CCGGGAUGUAAAAGUGC 17 6992

HSV-UL54-572 + CGGGAUGUAAAAGUGCA 17 6993

HSV-UL54-573 + GGGAUGUAAAAGUGCAU 17 6994 HSV-UL54-574 + GGAUGUAAAAGUGCAUC 17 6995

HSV-UL54-575 + GCCCCAACCCCCACGGU 17 6996

HSV-UL54-576 + CAACCCCCACGGUCGUG 17 6997

HSV-UL54-577 + AACCCCCACGGUCGUGU 17 6998

HSV-UL54-578 + ACCCCCACGGUCGUGUA 17 6999

HSV-UL54-579 + CCACGGUCGUGUAGUCG 17 7000

HSV-UL54-580 + CACGGUCGUGUAGUCGA 17 7001

HSV-UL54-581 + ACGGUCGUGUAGUCGAU 17 7002

HSV-UL54-582 + CGGUCGUGUAGUCGAUC 17 7003

HSV-UL54-583 + ACACGCCGCGCUCGACG 17 7004

HSV-UL54-584 + CGCUCGACGCGGUUGGC 17 7005

HSV-UL54-585 + GGAUGACCAACACAAAG 17 7006

HSV-UL54-586 + ACACAAAGGAGGCAAUA 17 7007

HSV-UL54-587 + UAAUGUCCGACAGGCGU 17 7008

HSV-UL54-588 + ACAGGCGUCGCCGCGAG 17 7009

HSV-UL54-589 + CGAGCACAGGUCGUCCA 17 7010

HSV-UL54-590 + GCCCGCACAGGCCUCGG 17 7011

HSV-UL54-591 + GCACAGGCCUCGGGCCU 17 7012

HSV-UL54-592 + CACAGGCCUCGGGCCUU 17 7013

HSV-UL54-593 + CGGGCCUUCAGGUAGCA 17 7014

HSV-UL54-594 + UCAGGUAGCACUGCAGA 17 7015

HSV-UL54-595 + GCACGGCGGCCGCCGUU 17 7016

HSV-UL54-596 + CCGUUCCGAUGAUAGGG 17 7017

HSV-UL54-597 + UUCCGAUGAUAGGGUCC 17 7018

HSV-UL54-598 + AUGAUAGGGUCCUGGGG 17 7019

HSV-UL54-599 + GGGGGCGGAGCGGCAGA 17 7020

HSV-UL54-600 + CGCCAGCGUCUCAUCCG 17 7021

HSV-UL54-601 + GCGUCUCAUCCGCGGAC 17 7022

HSV-UL54-602 + CGGACGCCAGGGCCUCG 17 7023

HSV-UL54-603 + GGGCCUCGAUGAGAUUU 17 7024

HSV-UL54-604 + CGAUGAGAUUUUCCUGG 17 7025

HSV-UL54-605 + GGCGCAGCACGCAGUCG 17 7026

HSV-UL54-606 + UGGCUGUCGACGCGGCC 17 7027

HSV-UL54-607 + GCGGGUUGGCUGCGAAU 17 7028

HSV-UL54-608 + CGGGUUGGCUGCGAAUG 17 7029

HSV-UL54-609 + UGAGCGACCAGGGUUUC 17 7030

HSV-UL54-610 + GGGUUUCCCAGG AAACC 17 7031

HSV-UL54-611 + AGGAAACCCGACGGGUC 17 7032

HSV-UL54-612 + GGAAACCCGACGGGUCU 17 7033

HSV-UL54-613 + GACGGGUCUCGGCGUCA 17 7034

HSV-UL54-614 + ACGGGUCUCGGCGUCAA 17 7035

HSV-UL54-615 + CCCCGCUUGGGUGGCCA 17 7036

HSV-UL54-616 + CCCGCUUGGGUGGCCAG 17 7037

HSV-UL54-617 + CCGCUUGGGUGGCCAGC 17 7038

HSV-UL54-618 + CGCUUGGGUGGCCAGCA 17 7039

HSV-UL54-619 + GCUUGGGUGGCCAGCAC 17 7040 HSV-UL54-620 + CU UGGGUGGCCAGCACG 17 7041

HSV-UL54-621 + GCACGGGAGCCCAGGGG 17 7042

HSV-UL54-622 + AGGGGCUGUUCGCGGCG 17 7043

HSV-UL54-623 + GGGGCUGUUCGCGGCGG 17 7044

HSV-UL54-624 + GCCAAAGGGGUCUUGCA 17 7045

HSV-UL54-625 + CCAAAGGGGUCUUGCAU 17 7046

HSV-UL54-626 + AAGGGGUCUUGCAUGAC 17 7047

HSV-UL54-627 + GGGCACUGCGU CCAAAG 17 7048

HSV-UL54-628 + AAGCUUUCGCUGAUGCG 17 7049

HSV-UL54-629 + CCGCGCGCUCGGAUAUG 17 7050

HSV-UL54-630 + CGCGCUCGGAUAUGGAU 17 7051

HSV-UL54-631 + CCCGAACGGCGGGGUCG 17 7052

HSV-UL54-632 + GAACGGCGGGGUCGAUG 17 7053

HSV-UL54-633 + AACGGCGGGGUCGAUGG 17 7054

HSV-UL54-634 + UUCGCUCCGGGACCGGG 17 7055

HSV-UL54-635 + GGACCGGGGCGCGGCCG 17 7056

HSV-UL54-636 + GGGCGCGGCCGUCCGCG 17 7057

HSV-UL54-637 + UCCGCGUGCGGGGGGGU 17 7058

HSV-UL54-638 + CGUGCGGGGGGGUCAGG 17 7059

HSV-UL54-639 + UCAGCGGAGGGGGGGCC 17 7060

HSV-UL54-640 + GGCGCGUGCCUCGUGGC 17 7061

HSV-UL54-641 + CGUGGCCGCGGGCCCCC 17 7062

HSV-UL54-642 + CGGGCCCCCGGAGACGU 17 7063

HSV-UL54-643 + CCGGAGACGUCGAGCUG 17 7064

HSV-UL54-644 + CGGAGACGUCGAGCUGC 17 7065

HSV-UL54-645 + GGAGACGUCGAGCUGCU 17 7066

HSV-UL54-646 + GAGACGUCGAGCUGCUC 17 7067

HSV-UL54-647 + CUCCGCGCCGCGCCUCG 17 7068

HSV-UL54-648 + UCCGCGCCGCGCCUCGC 17 7069

HSV-UL54-649 + CCGCGCCGCGCCUCGCC 17 7070

HSV-UL54-650 + CGCCGCGCCUCGCCGUG 17 7071

HSV-UL54-651 + CGCCUCGCCGUGGGUGG 17 7072

HSV-UL54-652 + CGCCGUGGGUGGCGCCG 17 7073

HSV-UL54-653 + UGGGUGGCGCCGGGGCC 17 7074

HSV-UL54-654 + GGGCCGUCCGUCCGCGC 17 7075

HSV-UL54-655 + UCCGUCCGCGCCGACGC 17 7076

HSV-UL54-656 + UCCGCGCCGACGCGGGG 17 7077

HSV-UL54-657 + CGCCGACGCGGGGUGGC 17 7078

HSV-UL54-658 + ACGCGGGGUGGCGACCC 17 7079

HSV-UL54-659 + CCUUGGUUGUGGGCGUU 17 7080

HSV-UL54-660 + CUUGGUUGUGGGCGUUU 17 7081

HSV-UL54-661 + UUGGUUGUGGGCGUUUC 17 7082

HSV-UL54-662 + UGGUUGUGGGCGUUUCU 17 7083 Table 4D provides exemplary targeting domains for knocking out the UL54 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4D

HSV-UL54-699 - GGGAGUGUUCCUCGUCGGAC 20 7109

HSV-UL54-700 - GUUCCUCGUCGGACGAGGAC 20 7110

HSV-UL54-6 - UUCCUCGUCGGACGAGGACA 20 6429

HSV-UL54-115 - GAGGACAUGGAAGACCCCUG 20 6538

HSV-UL54-116 - AUGGAAGACCCCUGCGGAGA 20 6539

HSV-UL54-704 - GGAAGACCCCUGCGGAGACG 20 7111

HSV-UL54-117 - GAAGACCCCUGCGGAGACGG 20 6540

HSV-UL54-706 - ACCCCUGCGGAGACGGAGGG 20 7112

HSV-UL54-119 - CCCCUGCGGAGACGGAGGGG 20 6542

HSV-UL54-708 - CAAAGGUCCCCCGGCCCGCC 20 7113

HSV-UL54-709 - AAGGUCCCCCGGCCCGCCCC 20 7114

HSV-UL54-710 - CCCCGAGGACGCCGGCACCC 20 7115

HSV-UL54-711 - ACGCCUCGCCCGGCAGCGCG 20 7116

HSV-UL54-125 - CGCCUCGCCCGGCAGCGCGG 20 6548

HSV-UL54-126 - GCCUCGCCCGGCAGCGCGGC 20 6549

HSV-UL54-127 - CCUCGCCCGGCAGCGCGGCG 20 6550

HSV-UL54-715 - CACCGGCGUGUGGUCGCGCC 20 7117

HSV-UL54-128 - ACCGGCGUGUGGUCGCGCCU 20 6551

HSV-UL54-717 - ACCAGGCGGUCGGCUUCCCC 20 7118

HSV-UL54-130 - CCAGGCGGUCGGCUUCCCCC 20 6553

HSV-UL54-131 - CAGGCGGUCGGCUUCCCCCC 20 6554

HSV-UL54-720 - GGCUUCCCCCCGGGAACCGC 20 7119

HSV-UL54-16 - GCUUCCCCCCGGGAACCGCA 20 6439

HSV-UL54-132 - CU UCCCCCCGGGAACCGCAC 20 6555

HSV-UL54-17 - U U CCCCCCG GG AACCG CACG 20 6440

HSV-UL54-18 - UCCCCCCGGGAACCGCACGG 20 6441

HSV-UL54-19 - CCCCCCGGGAACCGCACGGG 20 6442

HSV-UL54-726 - UCGACCAAGGCACCGCAUCC 20 7120

HSV-UL54-727 - CAAGGCACCGCAUCCCCGAG 20 7121

HSV-UL54-728 - CCGCAUCCCCGAGGCGGGCG 20 7122

HSV-UL54-729 - GGCGGGCGGCGAGGUCGCCG 20 7123

HSV-UL54-140 - GCGGGCGGCGAGGUCGCCGC 20 6563

HSV-UL54-731 - CGGCGAGGUCGCCGCCGGGG 20 7124

HSV-UL54-143 - GGCGAGGUCGCCGCCGGGGC 20 6566

HSV-UL54-733 + GCGGCGACCUCGCCGCCCGC 20 7125

HSV-UL54-27 + GGAUGCGGUGCCUUGGUCGA 20 6450

HSV-UL54-735 + UGCGGUUCCCGGGGGGAAGC 20 7126

HSV-UL54-736 + GGCGCGACCACACGCCGGUG 20 7127

HSV-UL54-737 + GCCCCGGCGGCGACCUCGCC 20 7128

HSV-UL54-738 + CCCCGGCGGCGACCUCGCCG 20 7129

HSV-UL54-739 + CG G CG ACCU CG CCG CCCGCC 20 7130

HSV-UL54-740 + CGCCCGCCUCGGGGAUGCGG 20 7131

HSV-UL54-741 + CUCGGGGAUGCGGUGCCUUG 20 7132

HSV-UL54-742 + CCCGUGCGGU UCCCGGGGGG 20 7133

HSV-UL54-743 + CCGUGCGGUUCCCGGGGGGA 20 7134

HSV-UL54-744 + CGUGCGGUUCCCGGGGGGAA 20 7135 HSV-UL54-745 + GUGCGGUUCCCGGGGGGAAG 20 7136

HSV-UL54-746 + GCGGUUCCCGGGGGGAAGCC 20 7137

HSV-UL54-747 + GGGGGGAAGCCGACCGCCUG 20 7138

HSV-UL54-748 + GGGGGAAGCCGACCGCCUGG 20 7139

HSV-UL54-749 + GGGGAAGCCGACCGCCUGGU 20 7140

HSV-UL54-750 + AGGCGCGACCACACGCCGGU 20 7141

HSV-UL54-751 + CGCCGCGCUGCCGGGCGAGG 20 7142

HSV-UL54-752 + GCCGCGCUGCCGGGCGAGGC 20 7143

HSV-UL54-753 + CCGCGCUGCCGGGCGAGGCG 20 7144

HSV-UL54-754 + CGCGCUGCCGGGCGAGGCGU 20 7145

HSV-UL54-173 + GGGUGCCGGCGUCCUCGGGG 20 6596

HSV-UL54-179 + GGGGCGGGCCGGGGGACCUU 20 6602

HSV-UL54-757 + GGCGGGCCGGGGGACCUUUG 20 7146

HSV-UL54-758 + CCCCUCCGUCUCCGCAGGGG 20 7147

HSV-UL54-759 + CCCUCCGUCUCCGCAGGGGU 20 7148

HSV-UL54-760 + CCGUCUCCGCAGGGGUCUUC 20 7149

HSV-UL54-761 + CGUCUCCGCAGGGGUCUUCC 20 7150

HSV-UL54-762 + UCUCCGCAGGGGUCUUCCAU 20 7151

HSV-UL54-763 + CUCCGCAGGGGUCUUCCAUG 20 7152

HSV-UL54-764 + CAGGGGUCUUCCAUGUCCUC 20 7153

HSV-UL54-765 + AGGGGUCUUCCAUGUCCUCG 20 7154

HSV-UL54-766 + UGUCCUCGUCCGACGAGGAA 20 7155

HSV-UL54-767 + GUCCUCGUCCGACGAGGAAC 20 7156

HSV-UL54-768 + GUCCGACGAGGAACACUCCC 20 7157

HSV-UL54-769 + CCGACGAGGAACACUCCCCG 20 7158

HSV-UL54-770 + CGAGGAACACUCCCCGCUGC 20 7159

HSV-UL54-771 + CGCUGCUGUCGGACUCGGGG 20 7160

HSV-UL54-772 + GCUGCUGUCGGACUCGGGGU 20 7161

HSV-UL54-773 + CUGCUGUCGGACUCGGGGUC 20 7162

HSV-UL54-774 + UGCUGUCGGACUCGGGGUCG 20 7163

HSV-UL54-775 + GGUCGUCGCGGCGGCCCUCC 20 7164

HSV-UL54-776 + CUCGUCCCGCUCCAGAGCGU 20 7165

HSV-UL54-777 + CGUCCCGCUCCAGAGCGUCC 20 7166

HSV-UL54-778 + GUCCCGCUCCAGAGCGUCCU 20 7167

HSV-UL54-779 + CCCGCUCCAGAGCGUCCUCC 20 7168

HSV-UL54-780 + CUCCAGAGCGUCCUCCUCGA 20 7169

HSV-UL54-781 + UCCAGAGCGUCCUCCUCGAG 20 7170

HSV-UL54-782 + GAGCGUCCUCCUCGAGCUCG 20 7171

HSV-UL54-783 + AGCGUCCUCCUCGAGCUCGC 20 7172

HSV-UL54-784 + CUCGAGCUCGCUGUCGGACA 20 7173

HSV-UL54-785 + CGAGCUCGCUGUCGGACAGG 20 7174

HSV-UL54-786 + GAGCUCGCUGUCGGACAGGU 20 7175

HSV-UL54-787 + GCUCGCUGUCGGACAGGUCC 20 7176

HSV-UL54-52 + UGUCGGACAGGUCCAAUCCU 20 6475

HSV-UL54-789 + UCCUAGGUCGAUUAGCAUAU 20 7177

HSV-UL54-790 + GGUCGAUUAGCAUAUCAAUG 20 7178 HSV-UL54-188 - AGGAGGACGCUCUGGAG 17 6611

HSV-UL54-190 - CGCUCUGGAGCGGGACG 17 6613

HSV-UL54-793 - UGAUAUGCUAAUCGACC 17 7179

HSV-UL54-794 - CCUGUCCGACAGCGAGC 17 7180

HSV-UL54-795 - UGUCCGACAGCGAGCUC 17 7181

HSV-UL54-55 - GUCCGACAGCGAGCUCG 17 6478

HSV-UL54-797 - GGACAUGGAAGACCCCU 17 7182

HSV-UL54-798 - GGAAGACCCCUGCGGAG 17 7183

HSV-UL54-799 - ACGCUCUGGAGCGGGAC 17 7184

HSV-UL54-194 - CCGAGUCCGACAGCAGC 17 6617

HSV-UL54-801 - UGCUAAUCGACCUAGGA 17 7185

HSV-UL54-802 - AUUGGACCUGUCCGACA 17 7186

HSV-UL54-803 - CCGACAGCGAGCUCGAG 17 7187

HSV-UL54-804 - AGCUCGAGGAGGACGCU 17 7188

HSV-UL54-57 - GCUCGAGGAGGACGCUC 17 6480

HSV-UL54-806 - GAGGAGGACGCUCUGGA 17 7189

HSV-UL54-807 - GGACGCUCUGGAGCGGG 17 7190

HSV-UL54-808 - CUCUGGAGCGGGACGAG 17 7191

HSV-UL54-809 - GGGCCGCCGCGACGACC 17 7192

HSV-UL54-810 - CCCCGAGUCCGACAGCA 17 7193

HSV-UL54-193 - CCCGAGUCCGACAGCAG 17 6616

HSV-UL54-195 - CGAGUCCGACAGCAGCG 17 6618

HSV-UL54-813 - GCGGGGAGUGUUCCUCG 17 7194

HSV-UL54-814 - GGAGUGUUCCUCGUCGG 17 7195

HSV-UL54-815 - AGUGUUCCUCGUCGGAC 17 7196

HSV-UL54-816 - CCUCGUCGGACGAGGAC 17 7197

HSV-UL54-58 - CUCGUCGGACGAGGACA 17 6481

HSV-UL54-198 - GACAUGGAAGACCCCUG 17 6621

HSV-UL54-199 - GAAGACCCCUGCGGAGA 17 6622

HSV-UL54-820 - AGACCCCUGCGGAGACG 17 7198

HSV-UL54-200 - GACCCCUGCGGAGACGG 17 6623

HSV-UL54-822 - CCUGCGGAGACGGAGGG 17 7199

HSV-UL54-202 - CUGCGGAGACGGAGGGG 17 6625

HSV-UL54-824 - AGGUCCCCCGGCCCGCC 17 7200

HSV-UL54-825 - GUCCCCCGGCCCGCCCC 17 7201

HSV-UL54-826 - CGAGGACGCCGGCACCC 17 7202

HSV-UL54-827 - CCUCGCCCGGCAGCGCG 17 7203

HSV-UL54-208 - CUCGCCCGGCAGCGCGG 17 6631

HSV-UL54-209 - UCGCCCGGCAGCGCGGC 17 6632

HSV-UL54-210 - CGCCCGGCAGCGCGGCG 17 6633

HSV-UL54-831 - CGGCGUGUGGUCGCGCC 17 7204

HSV-UL54-211 - GGCGUGUGGUCGCGCCU 17 6634

HSV-UL54-833 - AGGCGGUCGGCUUCCCC 17 7205

HSV-UL54-213 - GGCGGUCGGCUUCCCCC 17 6636

HSV-UL54-214 - GCGGUCGGCUUCCCCCC 17 6637

HSV-UL54-836 - UUCCCCCCGGGAACCGC 17 7206 HSV-UL54-68 - UCCCCCCGGGAACCGCA 17 6491

HSV-UL54-215 - CCCCCCGGGAACCGCAC 17 6638

HSV-UL54-69 - CCCCCGGGAACCGCACG 17 6492

HSV-UL54-70 - CCCCGGGAACCGCACGG 17 6493

HSV-UL54-71 - CCCGGGAACCGCACGGG 17 6494

HSV-UL54-842 - ACCAAGGCACCGCAUCC 17 7207

HSV-UL54-843 - GGCACCGCAUCCCCGAG 17 7208

HSV-UL54-844 - CAUCCCCGAGGCGGGCG 17 7209

HSV-UL54-845 - GGGCGGCGAGGUCGCCG 17 7210

HSV-UL54-223 - GGCGGCGAGGUCGCCGC 17 6646

HSV-UL54-847 - CGAGGUCGCCGCCGGGG 17 7211

HSV-UL54-226 - GAGGUCGCCGCCGGGGC 17 6649

HSV-UL54-849 + GCGGCGACCUCGCCGCC 17 7212

HSV-UL54-850 + GGAUGCGGUGCCUUGGU 17 7213

HSV-UL54-851 + UGCGGUUCCCGGGGGGA 17 7214

HSV-UL54-852 + GGCGCGACCACACGCCG 17 7215

HSV-UL54-853 + GCCCCGGCGGCGACCUC 17 7216

HSV-UL54-854 + CCCCGGCGGCGACCUCG 17 7217

HSV-UL54-855 + CGGCGACCUCGCCGCCC 17 7218

HSV-UL54-238 + CGCCCGCCUCGGGGAUG 17 6661

HSV-UL54-857 + CUCGGGGAUGCGGUGCC 17 7219

HSV-UL54-87 + CCCGUGCGGU UCCCGGG 17 6510

HSV-UL54-859 + CCGUGCGGUUCCCGGGG 17 7220

HSV-UL54-860 + CGUGCGGUUCCCGGGGG 17 7221

HSV-UL54-861 + GUGCGGUUCCCGGGGGG 17 7222

HSV-UL54-862 + GCGGUUCCCGGGGGGAA 17 7223

HSV-UL54-863 + GGGGGGAAGCCGACCGC 17 7224

HSV-UL54-244 + GGGGGAAGCCGACCGCC 17 6667

HSV-UL54-865 + GGGGAAGCCGACCGCCU 17 7225

HSV-UL54-866 + AGGCGCGACCACACGCC 17 7226

HSV-UL54-250 + CGCCGCGCUGCCGGGCG 17 6673

HSV-UL54-868 + GCCGCGCUGCCGGGCGA 17 7227

HSV-UL54-869 + CCGCGCUGCCGGGCGAG 17 7228

HSV-UL54-870 + CGCGCUGCCGGGCGAGG 17 7229

HSV-UL54-255 + GGGUGCCGGCGUCCUCG 17 6678

HSV-UL54-872 + GGGGCGGGCCGGGGGAC 17 7230

HSV-UL54-873 + GGCGGGCCGGGGGACCU 17 7231

HSV-UL54-265 + CCCCUCCGUCUCCGCAG 17 6688

HSV-UL54-875 + CCCUCCGUCUCCGCAGG 17 7232

HSV-UL54-876 + CCGUCUCCGCAGGGGUC 17 7233

HSV-UL54-877 + CGUCUCCGCAGGGGUCU 17 7234

HSV-UL54-878 + UCUCCGCAGGGGUCUUC 17 7235

HSV-UL54-879 + CUCCGCAGGGGUCUUCC 17 7236

HSV-UL54-880 + CAGGGGUCUUCCAUGUC 17 7237

HSV-UL54-881 + AGGGGUCUUCCAUGUCC 17 7238

HSV-UL54-882 + UGUCCUCGUCCGACGAG 17 7239 HSV-UL54-883 + GUCCUCGUCCGACGAGG 17 7240

HSV-UL54-884 + GUCCGACGAGGAACACU 17 7241

HSV-UL54-885 + CCG ACG AG G AACACU CC 17 7242

HSV-UL54-886 + CGAGGAACACUCCCCGC 17 7243

HSV-UL54-101 + CGCUGCUGUCGGACUCG 17 6524

HSV-UL54-888 + GCUGCUGUCGGACUCGG 17 7244

HSV-UL54-889 + CUGCUGUCGGACUCGGG 17 7245

HSV-UL54-890 + UGCUGUCGGACUCGGGG 17 7246

HSV-UL54-891 + GGUCGUCGCGGCGGCCC 17 7247

HSV-UL54-892 + CUCGUCCCGCUCCAGAG 17 7248

HSV-UL54-893 + CGUCCCGCUCCAGAGCG 17 7249

HSV-UL54-894 + GUCCCGCUCCAGAGCGU 17 7250

HSV-UL54-895 + CCCG CUCCAG AG CG U CC 17 7251

HSV-UL54-896 + CU CCAGAGCG UCCUCCU 17 7252

HSV-UL54-897 + UCCAGAGCGUCCUCCUC 17 7253

HSV-UL54-898 + GAGCGUCCUCCUCGAGC 17 7254

HSV-UL54-899 + AGCGUCCUCCUCGAGCU 17 7255

HSV-UL54-900 + CUCGAGCUCGCUGUCGG 17 7256

HSV-UL54-103 + CGAGCUCGCUGUCGGAC 17 6526

HSV-UL54-902 + GAGCUCGCUGUCGGACA 17 7257

HSV-UL54-903 + GCUCGCUGUCGGACAGG 17 7258

HSV-UL54-904 + UGUCGGACAGGUCCAAU 17 7259

HSV-UL54-905 + UCCUAGGUCGAUUAGCA 17 7260

HSV-UL54-906 + GGUCGAUUAGCAUAUCA 17 7261

Table 4E provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence, but downstream of the first 500bp of the UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a S. aureus Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using S. aureus Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4E

2nd Tier Target

DNA

g NA Name Targeting Domain Site Seq

Strand

Length ID

HSV-UL54-907 - CCACGGCGAGGCGCGGCGCG 20 7262

HSV-UL54-908 - CACCCACGGCGAGGCGCGGC 20 7263

HSV-UL54-281 - ACCCACGGCGAGGCGCGGCG 20 6704

HSV-UL54-910 - GUCUCCGGGGGCCCGCGGCC 20 7264

HSV-UL54-911 - GGUCGCCACCCCGCGUCGGC 20 7265

HSV-UL54-912 - GCCACCCCGCGUCGGCGCGG 20 7266

HSV-UL54-913 - CGGCCCCGGCGCCACCCACG 20 7267

HSV-UL54-282 - CACGGCGAGGCGCGGCGCGG 20 6705

HSV-UL54-283 - ACGGCGAGGCGCGGCGCGGA 20 6706

HSV-UL54-284 - CGGCGAGGCGCGGCGCGGAG 20 6707

HSV-UL54-917 - AGGGGAGCAGCUCGACGUCU 20 7268

HSV-UL54-285 - GGGGAGCAGCUCGACGUCUC 20 6708

HSV-UL54-286 - GGGAGCAGCUCGACGUCUCC 20 6709

HSV-UL54-920 - U G U CCCU G ACCCCCCCG CAC 20 7269

HSV-UL54-921 - CGGACGGCCGCGCCCCGGUC 20 7270

HSV-UL54-296 - GGACGGCCGCGCCCCGGUCC 20 6719

HSV-UL54-923 - GGCCGCGCCCCGGUCCCGGA 20 7271

HSV-UL54-924 - GACACCAUCGACCCCGCCGU 20 7272

HSV-UL54-925 - GGCGGUUCUGCGAUCCAUAU 20 7273

HSV-UL54-926 - CAUAUCCGAGCGCGCGGCGG 20 7274

HSV-UL54-927 - CGCGGCGGUCGAGCGCAUCA 20 7275

HSV-UL54-928 - CGAGCGCAUCAGCGAAAGCU 20 7276

HSV-UL54-929 - GGUCAUGCAAGACCCCUUUG 20 7277

HSV-UL54-306 - GUCAUGCAAGACCCCUUUGG 20 6729

HSV-UL54-931 - GCGGGAUGCCGUUUCCCGCC 20 7278

HSV-UL54-932 - U U UCCCGCCGCG AACAGCCC 20 7279

HSV-UL54-933 - CUCCCGUGCUGGCCACCCAA 20 7280

HSV-UL54-311 - UCCCGUGCUGGCCACCCAAG 20 6734

HSV-UL54-312 - CCCGUGCUGGCCACCCAAGC 20 6735

HSV-UL54-313 - CCGUGCUGGCCACCCAAGCG 20 6736

HSV-UL54-314 - CGUGCUGGCCACCCAAGCGG 20 6737

HSV-UL54-938 - CCAAGCGGGGGGGUUUGACG 20 7281

HSV-UL54-939 - GGGUUUGACGCCGAGACCCG 20 7282

HSV-UL54-940 - GCCGAGACCCGUCGGGUUUC 20 7283

HSV-UL54-319 - CCGAGACCCGUCGGGUUUCC 20 6742

HSV-UL54-320 - CGAGACCCGUCGGGUUUCCU 20 6743

HSV-UL54-943 - AAACCCUGGUCGCUCACGGC 20 7284

HSV-UL54-944 - CGCACAUUCGCAGCCAACCC 20 7285

HSV-UL54-945 - UGCGCGACUGCGUGCUGCGC 20 7286

HSV-UL54-326 - GCGCGACUGCGUGCUGCGCC 20 6749

HSV-UL54-947 - GCUGCGCCAGGAAAAUCUCA 20 7287

HSV-UL54-948 - UCAUCGAGGCCCUGGCGUCC 20 7288

HSV-UL54-671 - CGAGGCCCUGGCGUCCGCGG 20 7089 HSV-UL54-950 - ACAAUCUGCCGCUCCGCCCC 20 7289

HSV-UL54-951 - CCGCCCCCAGGACCCUAUCA 20 7290

HSV-UL54-333 - CGCCCCCAGGACCCUAUCAU 20 6756

HSV-UL54-953 - UCGGAACGGCGGCCGCCGUG 20 7291

HSV-UL54-336 - CGGAACGGCGGCCGCCGUGC 20 6759

HSV-UL54-955 - GCCCCUUUCUGCAGUGCUAC 20 7292

HSV-UL54-956 - CUGCAGUGCUACCUGAAGGC 20 7293

HSV-UL54-957 - CCUGAAGGCCCGAGGCCUGU 20 7294

HSV-UL54-958 - AGGCCCGAGGCCUGUGCGGG 20 7295

HSV-UL54-959 - UGUGCUCGCGGCGACGCCUG 20 7296

HSV-UL54-960 - GGCGACGCCUGUCGGACAUU 20 7297

HSV-UL54-961 - GGCCCGCCUCGCCAACCGCG 20 7298

HSV-UL54-962 - ACCGCGUCGAGCGCGGCGUG 20 7299

HSV-UL54-348 - CCGCGUCGAGCGCGGCGUGU 20 6771

HSV-UL54-964 - CGGAGAUCGACUACACGACC 20 7300

HSV-UL54-349 - GGAGAUCGACUACACGACCG 20 6772

HSV-UL54-350 - GAGAUCGACUACACGACCGU 20 6773

HSV-UL54-967 - CGACUACACGACCGUGGGGG 20 7301

HSV-UL54-353 - GACUACACGACCGUGGGGGU 20 6776

HSV-UL54-969 - GACCGUGGGGGUUGGGGCCG 20 7302

HSV-UL54-970 - AGACGAUGCACUUUUACAUC 20 7303

HSV-UL54-357 - GACGAUGCACUUUUACAUCC 20 6780

HSV-UL54-358 - ACGAUGCACUUUUACAUCCC 20 6781

HSV-UL54-973 - ACAUCCCGGGGGCCUGCAUG 20 7304

HSV-UL54-974 - GGCCUGCAUGGCGGGUCUCA 20 7305

HSV-UL54-975 - UGGCGGGUCUCAUUGAAAUA 20 7306

HSV-UL54-976 - AAAUACUGGACACGCACCGC 20 7307

HSV-UL54-365 - AAUACUGGACACGCACCGCC 20 6788

HSV-UL54-978 - GGAGUGUUCCAGUCGCGUGU 20 7308

HSV-UL54-377 + GGGAUGUAAAAGUGCAUCGU 20 6800

HSV-UL54-382 + ACCCCCACGGUCGUGUAGUC 20 6805

HSV-UL54-981 + ACACGCGACUGGAACACUCC 20 7309

HSV-UL54-370 + GGCGGUGCGUGUCCAGUAUU 20 6793

HSV-UL54-983 + GCGGUGCGUGUCCAGUAUUU 20 7310

HSV-UL54-984 + UAUUUCAAUGAGACCCGCCA 20 7311

HSV-UL54-985 + UGAGACCCGCCAUGCAGGCC 20 7312

HSV-UL54-986 + CAUGCAGGCCCCCGGGAUGU 20 7313

HSV-UL54-378 + GGAUGUAAAAGUGCAUCGUC 20 6801

HSV-UL54-988 + GAUGUAAAAGUGCAUCGUCU 20 7314

HSV-UL54-989 + CGGCCCCAACCCCCACGGUC 20 7315

HSV-UL54-990 + CCCCCACGGUCGUGUAGUCG 20 7316

HSV-UL54-385 + ACGGUCGUGUAGUCGAUCUC 20 6808

HSV-UL54-386 + CGGUCGUGUAGUCGAUCUCC 20 6809

HSV-UL54-993 + GGUCGUGUAGUCGAUCUCCG 20 7317

HSV-UL54-387 + ACACGCCGCGCUCGACGCGG 20 6810

HSV-UL54-995 + CACGCCGCGCUCGACGCGGU 20 7318 HSV-UL54-996 + CGCGGUUGGCGAGGCGGGCC 20 7319

HSV-UL54-997 + AAUGUCCGACAGGCGUCGCC 20 7320

HSV-UL54-998 + CAGGCGUCGCCGCGAGCACA 20 7321

HSV-UL54-999 + CCCGCACAGGCCUCGGGCCU 20 7322

HSV-UL54-1000 + ACAGGCCUCGGGCCUUCAGG 20 7323

HSV-UL54-1001 + GCCUUCAGGUAGCACUGCAG 20 7324

HSV-UL54-1002 + GUAGCACUGCAGAAAGGGGC 20 7325

HSV-UL54-399 + GCACGGCGGCCGCCGUUCCG 20 6822

HSV-UL54-1004 + CACGGCGGCCGCCGUUCCGA 20 7326

HSV-UL54-402 + AUGAUAGGGUCCUGGGGGCG 20 6825

HSV-UL54-1006 + UGAUAGGGUCCUGGGGGCGG 20 7327

HSV-UL54-1007 + GGGGCGGAGCGGCAGAUUGU 20 7328

HSV-UL54-1008 + CCGCGGACGCCAGGGCCUCG 20 7329

HSV-UL54-1009 + GGACGCCAGGGCCUCGAUGA 20 7330

HSV-UL54-1010 + UGAGAUUUUCCUGGCGCAGC 20 7331

HSV-UL54-409 + GGCGCAGCACGCAGUCGCGC 20 6832

HSV-UL54-1012 + GCGCAGCACGCAGUCGCGCA 20 7332

HSV-UL54-1013 + GGGUUGGCUGCGAAUGUGCG 20 7333

HSV-UL54-1014 + GCCGUGAGCGACCAGGGUUU 20 7334

HSV-UL54-415 + AGGAAACCCGACGGGUCUCG 20 6838

HSV-UL54-416 + GGAAACCCGACGGGUCUCGG 20 6839

HSV-UL54-1017 + GAAACCCGACGGGUCUCGGC 20 7335

HSV-UL54-1018 + CGGGUCUCGGCGUCAAACCC 20 7336

HSV-UL54-1019 + CG U CAAACCCCCCCGCU U GG 20 7337

HSV-UL54-421 + CCGCUUGGGUGGCCAGCACG 20 6844

HSV-UL54-422 + CGCUUGGGUGGCCAGCACGG 20 6845

HSV-UL54-423 + GCUUGGGUGGCCAGCACGGG 20 6846

HSV-UL54-424 + CUUGGGUGGCCAGCACGGGA 20 6847

HSV-UL54-1024 + UUGGGUGGCCAGCACGGGAG 20 7338

HSV-UL54-1025 + GGGCUGUUCGCGGCGGGAAA 20 7339

HSV-UL54-1026 + GGCGGGAAACGGCAUCCCGC 20 7340

HSV-UL54-429 + CCAAAGGGGUCUUGCAUGAC 20 6852

HSV-UL54-1028 + CAAAGGGGUCUUGCAUGACC 20 7341

HSV-UL54-1029 + AGCUUUCGCUGAUGCGCUCG 20 7342

HSV-UL54-1030 + UGAUGCGCUCGACCGCCGCG 20 7343

HSV-UL54-1031 + CCGCCGCGCGCUCGGAUAUG 20 7344

HSV-UL54-1032 + AUAUGGAUCGCAGAACCGCC 20 7345

HSV-UL54-1033 + ACGGCGGGGUCGAUGGUGUC 20 7346

HSV-UL54-1034 + UCCGGGACCGGGGCGCGGCC 20 7347

HSV-UL54-439 + GGACCGGGGCGCGGCCGUCC 20 6862

HSV-UL54-1036 + GACCGGGGCGCGGCCGUCCG 20 7348

HSV-UL54-1037 + GUGCGGGGGGGUCAGGGACA 20 7349

HSV-UL54-1038 + GGCCGCGGGCCCCCGGAGAC 20 7350

HSV-UL54-449 + GGAGACGUCGAGCUGCUCCC 20 6872

HSV-UL54-450 + GAGACGUCGAGCUGCUCCCC 20 6873

HSV-UL54-1041 + AGACGUCGAGCUGCUCCCCU 20 7351 HSV-UL54-451 + CUCCGCGCCGCGCCUCGCCG 20 6874

HSV-UL54-452 + UCCGCGCCGCGCCUCGCCGU 20 6875

HSV-UL54-453 + CCGCGCCGCGCCUCGCCGUG 20 6876

HSV-UL54-1045 + CGCGCCGCGCCUCGCCGUGG 20 7352

HSV-UL54-454 + CGCCGCGCCUCGCCGUGGGU 20 6877

HSV-UL54-1047 + GCCGCGCCUCGCCGUGGGUG 20 7353

HSV-UL54-1048 + CGUGGGUGGCGCCGGGGCCG 20 7354

HSV-UL54-1049 + CCGCGCCGACGCGGGGUGGC 20 7355

HSV-UL54-1050 + GCCGACGCGGGGUGGCGACC 20 7356

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-464 + CUUGGUUGUGGGCGUUUCUG 20 6887

HSV-UL54-1054 - CGGCGAGGCGCGGCGCG 17 7357

HSV-UL54-1055 - CCACGGCGAGGCGCGGC 17 7358

HSV-UL54-477 - CACGGCGAGGCGCGGCG 17 6898

HSV-UL54-1057 - UCCGGGGGCCCGCGGCC 17 7359

HSV-UL54-1058 - CGCCACCCCGCGUCGGC 17 7360

HSV-UL54-1059 - ACCCCGCGUCGGCGCGG 17 7361

HSV-UL54-1060 - CCCCGGCGCCACCCACG 17 7362

HSV-UL54-478 - GGCGAGGCGCGGCGCGG 17 6899

HSV-UL54-479 - GCGAGGCGCGGCGCGGA 17 6900

HSV-UL54-480 - CGAGGCGCGGCGCGGAG 17 6901

HSV-UL54-1064 - GGAGCAGCUCGACGUCU 17 7363

HSV-UL54-481 - GAGCAGCUCGACGUCUC 17 6902

HSV-UL54-482 - AGCAGCUCGACGUCUCC 17 6903

HSV-UL54-1067 - CCCUGACCCCCCCGCAC 17 7364

HSV-UL54-1068 - ACGGCCGCGCCCCGGUC 17 7365

HSV-UL54-492 - CGGCCGCGCCCCGGUCC 17 6913

HSV-UL54-1070 - CGCGCCCCGGUCCCGGA 17 7366

HSV-UL54-1071 - ACCAUCGACCCCGCCGU 17 7367

HSV-UL54-1072 - GGUUCUGCGAUCCAUAU 17 7368

HSV-UL54-1073 - AUCCGAGCGCGCGGCGG 17 7369

HSV-UL54-1074 - GGCGGUCGAGCGCAUCA 17 7370

HSV-UL54-1075 - GCGCAUCAGCGAAAGCU 17 7371

HSV-UL54-1076 - CAUGCAAGACCCCUUUG 17 7372

HSV-UL54-502 - AUGCAAGACCCCUUUGG 17 6923

HSV-UL54-1078 - GGAUGCCGUUUCCCGCC 17 7373

HSV-UL54-1079 - CCCGCCGCGAACAGCCC 17 7374

HSV-UL54-1080 - CCGUGCUGGCCACCCAA 17 7375

HSV-UL54-507 - CGUGCUGGCCACCCAAG 17 6928

HSV-UL54-508 - GUGCUGGCCACCCAAGC 17 6929

HSV-UL54-509 - UGCUGGCCACCCAAGCG 17 6930

HSV-UL54-510 - GCUGGCCACCCAAGCGG 17 6931

HSV-UL54-1085 - AGCGGGGGGGUUUGACG 17 7376

HSV-UL54-1086 - UUUGACGCCGAGACCCG 17 7377

HSV-UL54-1087 - GAGACCCGUCGGGUUUC 17 7378 HSV-UL54-515 - AGACCCGUCGGGUUUCC 17 6936

HSV-UL54-516 - GACCCGUCGGGUUUCCU 17 6937

HSV-UL54-1090 - CCCUGGUCGCUCACGGC 17 7379

HSV-UL54-1091 - ACAUUCGCAGCCAACCC 17 7380

HSV-UL54-1092 - GCGACUGCGUGCUGCGC 17 7381

HSV-UL54-522 - CGACUGCGUGCUGCGCC 17 6943

HSV-UL54-1094 - GCGCCAGGAAAAUCUCA 17 7382

HSV-UL54-1095 - UCGAGGCCCUGGCGUCC 17 7383

HSV-UL54-1096 - GGCCCUGGCGUCCGCGG 17 7384

HSV-UL54-1097 - AUCUGCCGCUCCGCCCC 17 7385

HSV-UL54-1098 - CCCCCAGGACCCUAUCA 17 7386

HSV-UL54-529 - CCCCAGGACCCUAUCAU 17 6950

HSV-UL54-1100 - GAACGGCGGCCGCCGUG 17 7387

HSV-UL54-532 - AACGGCGGCCGCCGUGC 17 6953

HSV-UL54-1102 - CCUU UCUGCAGUGCUAC 17 7388

HSV-UL54-1103 - CAGUGCUACCUGAAGGC 17 7389

HSV-UL54-1104 - GAAGGCCCGAGGCCUGU 17 7390

HSV-UL54-1105 - CCCGAGGCCUGUGCGGG 17 7391

HSV-UL54-1106 - GCUCGCGGCGACGCCUG 17 7392

HSV-UL54-1107 - GACGCCUGUCGGACAUU 17 7393

HSV-UL54-1108 - CCGCCUCGCCAACCGCG 17 7394

HSV-UL54-1109 - GCGUCGAGCGCGGCGUG 17 7395

HSV-UL54-544 - CGUCGAGCGCGGCGUGU 17 6965

HSV-UL54-1111 - AGAUCGACUACACGACC 17 7396

HSV-UL54-545 - GAUCGACUACACGACCG 17 6966

HSV-UL54-546 - AUCGACUACACGACCGU 17 6967

HSV-UL54-1114 - CUACACGACCGUGGGGG 17 7397

HSV-UL54-549 - UACACGACCGUGGGGGU 17 6970

HSV-UL54-1116 - CGUGGGGGUUGGGGCCG 17 7398

HSV-UL54-1117 - CGAUGCACUUUUACAUC 17 7399

HSV-UL54-553 - GAUGCACUUUUACAUCC 17 6974

HSV-UL54-554 - AUGCACUUUUACAUCCC 17 6975

HSV-UL54-1120 - UCCCGGGGGCCUGCAUG 17 7400

HSV-UL54-1121 - CUGCAUGGCGGGUCUCA 17 7401

HSV-UL54-1122 - CGGGUCUCAUUGAAAUA 17 7402

HSV-UL54-1123 - UACUGGACACGCACCGC 17 7403

HSV-UL54-561 - ACUGGACACGCACCGCC 17 6982

HSV-UL54-1125 - GUGUUCCAGUCGCGUGU 17 7404

HSV-UL54-573 + GGGAUGUAAAAGUGCAU 17 6994

HSV-UL54-578 + ACCCCCACGGUCGUGUA 17 6999

HSV-UL54-1128 + ACACGCG ACU G G AACAC 17 7405

HSV-UL54-566 + GGCGGUGCGUGUCCAGU 17 6987

HSV-UL54-1130 + GCGGUGCGUGUCCAGUA 17 7406

HSV-UL54-1131 + UAU U UCAAUG AGACCCG 17 7407

HSV-UL54-1132 + UGAGACCCGCCAUGCAG 17 7408

HSV-UL54-1133 + CAUGCAGGCCCCCGGGA 17 7409 HSV-UL54-574 + GGAUGUAAAAGUGCAUC 17 6995

HSV-UL54-1135 + GAUGUAAAAGUGCAUCG 17 7410

HSV-UL54-1136 + CGGCCCCAACCCCCACG 17 7411

HSV-UL54-1137 + CCCCCACGGUCGUGUAG 17 7412

HSV-UL54-581 + ACGGUCGUGUAGUCGAU 17 7002

HSV-UL54-582 + CGGUCGUGUAGUCGAUC 17 7003

HSV-UL54-1140 + GGUCGUGUAGUCGAUCU 17 7413

HSV-UL54-583 + ACACGCCGCGCUCGACG 17 7004

HSV-UL54-1142 + CACGCCGCGCUCGACGC 17 7414

HSV-UL54-1143 + CGCGGU UGGCGAGGCGG 17 7415

HSV-UL54-1144 + AAUGU CCGACAGGCG U C 17 7416

HSV-UL54-1145 + CAGGCGUCGCCGCGAGC 17 7417

HSV-UL54-1146 + CCCGCACAGGCCUCGGG 17 7418

HSV-UL54-1147 + ACAGGCCUCGGGCCUUC 17 7419

HSV-UL54-1148 + GCCUUCAGGUAGCACUG 17 7420

HSV-UL54-1149 + GUAGCACUGCAGAAAGG 17 7421

HSV-UL54-595 + GCACGGCGGCCGCCGUU 17 7016

HSV-UL54-1151 + CACGGCGGCCGCCGUUC 17 7422

HSV-UL54-598 + AUGAUAGGGUCCUGGGG 17 7019

HSV-UL54-1153 + UGAUAGGGUCCUGGGGG 17 7423

HSV-UL54-1154 + GGGGCGGAGCGGCAGAU 17 7424

HSV-UL54-1155 + CCGCGGACGCCAGGGCC 17 7425

HSV-UL54-1156 + GGACGCCAGGGCCUCGA 17 7426

HSV-UL54-1157 + UGAGAUUUUCCUGGCGC 17 7427

HSV-UL54-605 + GGCGCAGCACGCAGUCG 17 7026

HSV-UL54-1159 + GCGCAGCACGCAGUCGC 17 7428

HSV-UL54-1160 + GGGUUGGCUGCGAAUGU 17 7429

HSV-UL54-1161 + GCCGUGAGCGACCAGGG 17 7430

HSV-UL54-611 + AGGAAACCCGACGGGUC 17 7032

HSV-UL54-612 + GGAAACCCGACGGGUCU 17 7033

HSV-UL54-1164 + GAAACCCGACGGGUCUC 17 7431

HSV-UL54-1165 + CGGGUCUCGGCGUCAAA 17 7432

HSV-UL54-1166 + CGUCAAACCCCCCCGCU 17 7433

HSV-UL54-617 + CCGCUUGGGUGGCCAGC 17 7038

HSV-UL54-618 + CGCUUGGGUGGCCAGCA 17 7039

HSV-UL54-619 + GCUUGGGUGGCCAGCAC 17 7040

HSV-UL54-620 + CU UGGGUGGCCAGCACG 17 7041

HSV-UL54-1171 + UUGGGUGGCCAGCACGG 17 7434

HSV-UL54-1172 + GGGCUGUUCGCGGCGGG 17 7435

HSV-UL54-1173 + GGCGGGAAACGGCAUCC 17 7436

HSV-UL54-625 + CCAAAGGGGUCUUGCAU 17 7046

HSV-UL54-1175 + CAAAGGGGUCUUGCAUG 17 7437

HSV-UL54-1176 + AGCUUUCGCUGAUGCGC 17 7438

HSV-UL54-1177 + UGAUGCGCUCGACCGCC 17 7439

HSV-UL54-1178 + CCGCCGCGCGCUCGGAU 17 7440

HSV-UL54-1179 + AUAUGGAUCGCAGAACC 17 7441 HSV-UL54-1180 + ACGGCGGGGUCGAUGGU 17 7442

HSV-UL54-1181 + UCCGGGACCGGGGCGCG 17 7443

HSV-UL54-635 + GGACCGGGGCGCGGCCG 17 7056

HSV-UL54-1183 + GACCGGGGCGCGGCCGU 17 7444

HSV-UL54-1184 + GUGCGGGGGGGUCAGGG 17 7445

HSV-UL54-1185 + GGCCGCGGGCCCCCGGA 17 7446

HSV-UL54-645 + GGAGACGUCGAGCUGCU 17 7066

HSV-UL54-646 + GAGACGUCGAGCUGCUC 17 7067

HSV-UL54-1188 + AGACGUCGAGCUGCUCC 17 7447

HSV-UL54-647 + CUCCGCGCCGCGCCUCG 17 7068

HSV-UL54-648 + UCCGCGCCGCGCCUCGC 17 7069

HSV-UL54-649 + CCGCGCCGCGCCUCGCC 17 7070

HSV-UL54-1192 + CGCGCCGCGCCUCGCCG 17 7448

HSV-UL54-650 + CGCCGCGCCUCGCCGUG 17 7071

HSV-UL54-1194 + GCCGCGCCUCGCCGUGG 17 7449

HSV-UL54-1195 + CGUGGGUGGCGCCGGGG 17 7450

HSV-UL54-1196 + CCGCGCCGACGCGGGGU 17 7451

HSV-UL54-1197 + GCCGACGCGGGGUGGCG 17 7452

HSV-UL54-661 + UUGGUUGUGGGCGUUUC 17 7082

HSV-UL54-662 + UGGUUGUGGGCGUUUCU 17 7083

HSV-UL54-1200 + GGUUGUGGGCGUUUCUG 17 7453

Table 4F provides exemplary targeting domains for knocking out the UL54 gene selected according to the first tier parameters. The targeting domains are selected based on location within first 500bp of the coding sequence of the UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single-stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4F

HSV-UL54-8 - AGGGGCGGAGGCCAUCGACG 20 6431

HSV-UL54-665 + CGUCGAUGGCCUCCGCCCCU 20 7085

HSV-UL54-187 + GUCGAUUAGCAUAUCAAUGU 20 6610

HSV-UL54-667 - UUGAUAUGCUAAUCGAC 17 7086

HSV-UL54-60 - GGCGGAGGCCAUCGACG 17 6483

HSV-UL54-669 + CGUCGAUGGCCUCCGCC 17 7087

HSV-UL54-670 + GUCGAUUAGCAUAUCAA 17 7088

Table 4G provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains are selected based on location within the coding sequence (but downstream of the first 500bp) of UL54 gene. It is contemplated herein that the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that gives double stranded cleavage. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 single- stranded break nucleases (nickases). In an embodiment, dual targeting is used to create two nicks on opposite DNA strands by using N. meningitidis Cas9 nickases with two targeting domains that are complementary to opposite DNA strands, e.g., a gRNA comprising any minus strand targeting domain may be paired any gRNA comprising a plus strand targeting domain provided that the two gRNAs are oriented on the DNA such that PAMs face outward and the distance between the 5' ends of the gRNAs is 0-50bp.

Table 4G

Table 5 A provides exemplary targeting domains for knocking out the UL19 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 5A

Table 5B provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 5B

HSV2-UL19-75 + CGUCAAACUCUACGUCAUAC 20 408

HSV2-UL19-92 + CCGCGGCGUACCGGUAACCC 20 507

HSV2-UL19-1548 + CAAACGCCCCCAGGACGGCC 20 1788

HSV2-UL19-49 + UGAAGGCGGCGU UCAGGGCC 20 476

HSV2-UL19-66 + CGUUCAUGUAAGCCAGCUCC 20 406

HSV2-UL19-21 - UGACGAAGGUCAUCGACCGC 20 397

HSV2-UL19-97 + CCGGUAACCCGGGGGGUCGC 20 511

HSV2-UL19-98 + UAACCCGGGGGGUCGCGGGC 20 413

HSV2-UL19-36 + UGGCGCGCAGCUGGCGAUGC 20 464

HSV2-UL19-8 - CGUAGAGU UUGACGCCCUGC 20 389

HSV2-UL19-82 + ACGCCACCUCGAUCGUACUC 20 500

HSV2-UL19-65 + UCGUUCAUGUAAGCCAGCUC 20 405

HSV2-UL19-47 + CCAGGCUGAAGGCGGCGUUC 20 474

HSV2-UL19-11 - CCUGUCGCUCGUGCGCU UUC 20 391

HSV2-UL19-27 - AGGCCAUUGCCCUGCUCACG 20 455

HSV2-UL19-93 + CGCGGCGUACCGGUAACCCG 20 508

HSV2-UL19-4 - CCCGGGU UACCGGUACGCCG 20 444

HSV2-UL19-6 - CUCCAUCCUGAGUACGAUCG 20 387

HSV2-UL19-58 + CGACGGGGUGCGGGCCGUCG 20 481

HSV2-UL19-96 + ACCGGUAACCCGGGGGGUCG 20 412

HSV2-UL19-7 - CAUCCUGAGUACGAUCGAGG 20 388

HSV2-UL19-50 + AGGCGGCGUUCAGGGCCCGG 20 477

HSV2-UL19-85 + CGUACUCAGGAUGGAGCCGG 20 411

HSV2-UL19-95 + CGGCGUACCGGUAACCCGGG 20 510

HSV2-UL19-56 + ACGGGCUGCUCGACGGGGUG 20 403

HSV2-UL19-3083 + AGCGGCCAUUGGGUUCCUUG 20 7466

HSV2-UL19-3084 + UCGCGGGCAGGAGCGGCCAU 20 7467

HSV2-UL19-3085 - CGCAACGUCCAGGCCGUCCU 20 7468

HSV2-UL19-12 - CUCGUGCGCUUUCUGGAGCU 20 392

HSV2-UL19-3086 + CGCGGGCAGGAGCGGCCAUU 20 7469

Table 5C provides exemplary targeting domains for knocking out the UL19 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 5C

HSV2-UL19-167 + G G U G CACACG CACG CCA 17 560

HSV2-UL19-173 + GCAGUAGGACCCCAGCA 17 565

HSV2-UL19-139 + GGCCUCCCCCGUGAGCA 17 539

HSV2-UL19-124 - GGCCAUUGCCCUGCUCA 17 525

HSV2-UL19-147 + GCUGAAGGCGGCGUUCA 17 547

HSV2-UL19-119 - GCAUAAUUACAUGACGA 17 423

HSV2-UL19-187 + GGAGCCGGUGGGCAGGA 17 575

HSV2-UL19-132 - GGAGGCCCUGGACGGGA 17 533

HSV2-UL19-3087 + GCGCGCGAGCUGCUGGA 17 7470

HSV2-UL19-104 - GCGGCCAUCCUGCCCAC 17 517

HSV2-UL19-151 + GUCAUGUAAUUAUGCAC 17 550

HSV2-UL19-125 - GCCAUUGCCCUGCUCAC 17 526

HSV2-UL19-133 - GAGGCCCUGGACGGGAC 17 534

HSV2-UL19-129 - GCUCACGGGGGAGGCCC 17 530

HSV2-UL19-134 + GCUGGAUGGCGCGCAGC 17 535

HSV2-UL19-138 + GGGCCUCCCCCGUGAGC 17 538

HSV2-UL19-158 + GGGGUGCGGGCCGUCGC 17 554

HSV2-UL19-186 + GGAUGGAGCCGGUGGGC 17 574

HSV2-UL19-156 + GCUGCUCGACGGGGUGC 17 552

HSV2-UL19-146 + GGCUGAAGGCGGCGUUC 17 546

HSV2-UL19-198 + GGGGUCGCGGGCAGGAG 17 584

HSV2-UL19-154 + GCACGGGCUGCUCGACG 17 429

HSV2-UL19-188 + GGUGGGCAGGAUGGCCG 17 576

HSV2-UL19-112 - GGAGCUCGGCCUGUCCG 17 519

HSV2-UL19-145 + GGUGGCCAGGCUGAAGG 17 545

HSV2-UL19-163 + GGGCGAUGAGGGGCUGG 17 558

HSV2-UL19-3088 + GCCAUUGGGUUCCUUGG 17 7471

HSV2-UL19-155 + GGCUGCUCGACGGGGUG 17 430

HSV2-UL19-3089 + GGCCAUUGGGUUCCUUG 17 7472

HSV2-UL19-141 + GAGCAGGGCAAUGGCCU 17 541

HSV2-UL19-3090 + GCGGCCAUUGGGUUCCU 17 7473

HSV2-UL19-111 - GUGCGCUUUCUGGAGCU 17 419

HSV2-UL19-108 - GAGUUUGACGCCCUGCU 17 417

HSV2-UL19-3091 + GGGCAGGAGCGGCCAUU 17 7474

HSV2-UL19-74 + GUUGCAGUAGGACCCCAGCA 20 493

HSV2-UL19-88 + GAUGGAGCCGGUGGGCAGGA 20 503

HSV2-UL19-33 - GGGGGAGGCCCUGGACGGGA 20 461

HSV2-UL19-45 + GGCCUCGGUGGCCAGGCUGA 20 472

HSV2-UL19-5 - GCCGCGGCCAUCCUGCCCAC 20 445

HSV2-UL19-26 - GAGGCCAUUGCCCUGCUCAC 20 454

HSV2-UL19-34 - GGGGAGGCCCUGGACGGGAC 20 462

HSV2-UL19-2 - GCUCCUGCCCGCGACCCCCC 20 442

HSV2-UL19-3092 - GCGCAACGUCCAGGCCGUCC 20 7475

HSV2-UL19-35 + GCUGCUGGAUGGCGCGCAGC 20 463

HSV2-UL19-84 + GAUCGUACUCAGGAUGGAGC 20 410

HSV2-UL19-59 + GACGGGGUGCGGGCCGUCGC 20 482 HSV2-UL19-89 + GCCGGUGGGCAGGAUGGCCG 20 504

HSV2-UL19-43 + GAGCAGGGCAAUGGCCUCGG 20 470

HSV2-UL19-3093 + GCGGCCAUUGGGUUCCUUGG 20 7476

HSV2-UL19-3094 + GGAGCGGCCAUUGGGUUCCU 20 7477

HSV2-UL19-72 + GAGCGACAGGGUGUUGCAGU 20 491

HSV2-UL19-86 + GUACUCAGGAUGGAGCCGGU 20 501

HSV2-UL19-3095 + GAGCGGCCAUUGGGUUCCUU 20 7478

Table 5D provides exemplary targeting domains for knocking out the UL19 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 5D

HSV2-UL19-172 + UGCAGUAGGACCCCAGC 17 564

HSV2-UL19-114 - CACCAAG U U CCCGGAGC 17 421

HSV2-UL19-183 + CGUACUCAGGAUGGAGC 17 437

HSV2-UL19-

+ U UCCUUGGGGGAUUCGC 17

3098 7481

HSV2-UL19-197 + CCCGGGGGGUCGCGGGC 17 440

HSV2-UL19-162 + CGCGGGCGAUGAGGGGC 17 557

HSV2-UL19-135 + CGCGCAGCUGGCGAUGC 17 536

HSV2-UL19-107 - AGAGUU UGACGCCCUGC 17 416

HSV2-UL19-164 + UUCAUGUAAGCCAGCUC 17 432

HSV2-UL19-161 + CCGUCGCGGGCGAUGAG 17 556

HSV2-UL19-126 - CCAUUGCCCUGCUCACG 17 527

HSV2-UL19-123 - CUUCAGCCUGGCCACCG 17 524

HSV2-UL19-157 + CGGGGUGCGGGCCGUCG 17 553

HSV2-UL19-117 - AGGGCGUGUGCAGUUCG 17 521

HSV2-UL19-127 - CAU UGCCCUGCUCACGG 17 528

HSV2-UL19-149 + CGGCGUUCAGGGCCCGG 17 549

HSV2-UL19-184 + ACUCAGGAUGGAGCCGG 17 438

HSV2-UL19-179 + AAUCAAAGAGUCUGCGG 17 570

HSV2-UL19-142 + CAGGGCAAUGGCCUCGG 17 542

HSV2-UL19-128 - UGCCCUGCUCACGGGGG 17 529

HSV2-UL19-723 - CGUCCAGGCCGUCCUGG 17 963

HSV2-UL19-109 - AGUUUGACGCCCUGCUG 17 518

HSV2-UL19-178 + AAAAAUCAAAGAGUCUG 17 569

HSV2-UL19-

+ CGGGCAGGAGCGGCCAU 17

3099 7482

HSV2-UL19-166 + AGCCAGCUCCGGGAACU 17 559

HSV2-UL19-171 + CGACAGGGUGUUGCAGU 17 563

HSV2-UL19-175 + AUACAGGCUGUUU UCGU 17 566

HSV2-UL19-185 + CUCAGGAUGGAGCCGGU 17 573

HSV2-UL19-180 + AUCAAAGAGUCUGCGGU 17 571

HSV2-UL19-41 + CU CCCCCG U G AG CAG G G CAA 20 468

HSV2-UL19-

- UGGAGCCUGCGAAUCCCCCA 20

3100 7483

HSV2-UL19-40 + CAGGGCCUCCCCCGUGAGCA 20 467

HSV2-UL19-25 - CGAGGCCAUUGCCCUGCUCA 20 453

HSV2-UL19-19 - CAGCCCCUCAUCGCCCGCGA 20 450

HSV2-UL19-83 + CACCUCGAUCGUACUCAGGA 20 409

HSV2-UL19-31 - CUCACGGGGGAGGCCCUGGA 20 459

HSV2-UL19-69 + U G CACACG CACG CCACG G AC 20 489

HSV2-UL19-32 - UCACGGGGGAGGCCCUGGAC 20 460

HSV2-UL19-3 - CCCGCG ACCCCCCGG G U U AC 20 443

HSV2-UL19-1 - CGCUCCUGCCCGCGACCCCC 20 441

HSV2-UL19-30 - CCUGCUCACGGGGGAGGCCC 20 458

HSV2-UL19-23 - CCUGAACGCCGCCUUCAGCC 20 451

HSV2-UL19-73 + UGUUGCAGUAGGACCCCAGC 20 492 HSV2-UL19-39 + CCAGGGCCUCCCCCGUGAGC 20 466

HSV2-UL19-63 + CGUCGCGGGCGAUGAGGGGC 20 485

HSV2-UL19-87 + UCAGGAUGGAGCCGGUGGGC 20 502

HSV2-UL19-57 + CGGGCUGCUCGACGGGGUGC 20 480

HSV2-UL19-99 + CGGGGGGUCGCGGGCAGGAG 20 512

HSV2-UL19-55 + UAUGCACGGGCUGCUCGACG 20 402

HSV2-UL19-24 - CGCCUUCAGCCUGGCCACCG 20 452

HSV2-UL19-13 - UCUGGAGCUCGGCCUGUCCG 20 447

HSV2-UL19-18 - CGAAGGGCGUGUGCAGUUCG 20 449

HSV2-UL19-46 + CUCGGUGGCCAGGCUGAAGG 20 473

HSV2-UL19-80 + A A A A A U C A A AG AGUCUGCGG 20 498

HSV2-UL19-29 - CAU UGCCCUGCUCACGGGGG 20 457

HSV2-UL19-345 - CAACGUCCAGGCCGUCCUGG 20 585

HSV2-UL19-64 + CGCGGGCGAUGAGGGGCUGG 20 486

HSV2-UL19-10 - UAGAGUU UGACGCCCUGCUG 20 446

HSV2-UL19-79 + CG AAAAAAUCAAAG AG U CU G 20 497

HSV2-UL19-42 + CGUGAGCAGGGCAAUGGCCU 20 469

HSV2-UL19-81 + AAAAUCAAAGAGUCUGCGGU 20 499

Table 5E provides exemplary targeting domains for knocking out the UL19 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 5E

HSV2-UL19-764 - GGCGAGAUGGUCUUGAA 17 1004

HSV2-UL19-1099 - CGACGCCUCCCCGCUAA 17 1339

HSV2-UL19-1649 + G U U G CCCAU GU CCG U AA 17 1889

HSV2-UL19-953 - GGUGCUGGCGCACAACA 17 1193

HSV2-UL19-946 - CAAUCUGGUGGCCAACA 17 1186

HSV2-UL19-939 - CGCCACGCUGCAGAACA 17 1179

HSV2-UL19-1618 + GGCGAUGAACUCACACA 17 1858

HSV2-UL19-1890 + GAGCGAAGGAUGACACA 17 2130

HSV2-UL19-1020 - CAACCCCGUUACGGACA 17 1260

HSV2-UL19-1058 - CGUGUACGCGGGGGACA 17 1298

HSV2-UL19-1815 + CACCAGGUCCCGAUACA 17 2055

HSV2-UL19-1677 + CUUGAGCUGGUGAUACA 17 1917

HSV2-UL19-1634 + CGCGUUGCGCAGGUACA 17 1874

HSV2-UL19-1924 + GUCCAUGGCGCCCACCA 17 2164

HSV2-UL19-1684 + GCGGGCGUGCUGCACCA 17 1924

HSV2-UL19-1063 - GCCCUCAUGUACGACCA 17 1303

HSV2-UL19-1979 + CUUCAGCUCGGCGACCA 17 2219

HSV2-UL19-733 - CGGGCGCCUGGCGACCA 17 973

HSV2-UL19-1699 + CAGGGCCGGGGGGACCA 17 1939

HSV2-UL19-1042 - CGCGCCGGCAUGGACCA 17 1282

HSV2-UL19-747 - GCUCGUGGACCUGACCA 17 987

HSV2-UL19-1692 + GAAGUAGUUGGCCCCCA 17 1932

HSV2-UL19-1918 + CGGGUUGAACAGCCCCA 17 2158

HSV2-UL19-1594 + AACCG CU GCG ACG CCCA 17 1834

HSV2-UL19-834 - CGGCAGCGCCUCGCCCA 17 1074

HSV2-UL19-867 - CGUUGGCCGCCACGCCA 17 1107

HSV2-UL19-1843 + CACCG G ACACAG CG CCA 17 2083

HSV2-UL19-1824 + AGUCACGAGCCGCGCCA 17 2064

HSV2-UL19-1835 + UAUGGUAGCCGGCGCCA 17 2075

HSV2-UL19-795 - CCCCCUGGUGGGCGCCA 17 1035

HSV2-UL19-1968 + CAGGCGGGGCACGGCCA 17 2208

HSV2-UL19-800 - GCUGUUCAACCCGGCCA 17 1040

HSV2-UL19-1017 - CACCGCCGUCGCGGCCA 17 1257

HSV2-UL19-1067 - GCGGCCCUUCGCGGCCA 17 1307

HSV2-UL19-1678 + CUGGUGAUACAGGGCCA 17 1918

HSV2-UL19-1989 + GGGCAACAGCAGGGCCA 17 2229

HSV2-UL19-1811 + GUCCACCAGCUGGGCCA 17 2051

HSV2-UL19-1046 - CGCCACCCCCGUGGCCA 17 1286

HSV2-UL19-1613 + GUUGAUGUCCGUGGCCA 17 1853

HSV2-UL19-1088 - GGGAUCGGCGGUAUCCA 17 1328

HSV2-UL19-1914 + CGCGGCGAAGCGCUCCA 17 2154

HSV2-UL19-1933 + GUAGAUGCGUCUCUCCA 17 2173

HSV2-UL19-1891 + CCCGACCGCGUUCUCCA 17 2131

HSV2-UL19-1922 + GACGAACGUCAGGUCCA 17 2162

HSV2-UL19-1793 + GUCUCGGUGGCGGUCCA 17 2033

HSV2-UL19-814 - GGUUCUGCGGCUGUCCA 17 1054 HSV2-UL19-1585 + G UGAAGAACUUGAAG CA 17 1825

HSV2-UL19-1987 + UUGCAUGGG C AAC AG CA 17 2227

HSV2-UL19-1736 + CACCUGCAGCGUGAGCA 17 1976

HSV2-UL19-1560 + GCGGGCGCUGCGUAGCA 17 1800

HSV2-UL19-1570 + UCCUGAAACAGGCCGCA 17 1810

HSV2-UL19-1946 + GUCGUCCAGGCUCCGCA 17 2186

HSV2-UL19-1704 + GCGCGCCAGGUCGCGCA 17 1944

HSV2-UL19-1807 + CAGCUCCGGGCCCGGCA 17 2047

HSV2-UL19-1041 - GCCGCGGCGCGCCGGCA 17 1281

HSV2-UL19-725 - GGCGUUUGAGCGCGGCA 17 965

HSV2-UL19-1967 + GUGCGUCAGGCGGGGCA 17 2207

HSV2-UL19-771 - GGCGCUGGUGAUGGGCA 17 1011

HSV2-UL19-740 - GAGCUUCUUCCUGGGCA 17 980

HSV2-UL19-886 - CCUCCCCGAGGAGUGCA 17 1126

HSV2-UL19-1984 + GUCGAGAUAUCGUUGCA 17 2224

HSV2-UL19-1610 + CGUCCCCGCGGGUUGCA 17 1850

HSV2-UL19-1081 - CACCGCGGCCGACAUCA 17 1321

HSV2-UL19-983 - GCUGACCUACGCGCUCA 17 1223

HSV2-UL19-963 - CGCCGGCGUGCUGCUCA 17 1203

HSV2-UL19-767 - CGGGGCCAACCUCGUCA 17 1007

HSV2-UL19-1752 + GUGGGCGGGGUCGGUCA 17 1992

HSV2-UL19-875 - CUGCAAGCCGCGAUUCA 17 1115

HSV2-UL19-988 - GUAUCACCAGCUCAAGA 17 1228

HSV2-UL19-1766 + GCAGCGCCCCCGCGAGA 17 2006

HSV2-UL19-763 - GGUGACCUACGGCGAGA 17 1003

HSV2-UL19-727 - GCACGUGCUGUUGGAGA 17 967

HSV2-UL19-1786 + GCACGCCGCCGCGUAGA 17 2026

HSV2-UL19-921 - GACUUUAACCGCAACGA 17 1161

HSV2-UL19-1796 + GUCGCAGUCCCACACGA 17 2036

HSV2-UL19-1669 + GCGGUCCUGCCGCACGA 17 1909

HSV2-UL19-954 - CAUGGCCGAGCGCACGA 17 1194

HSV2-UL19-1888 + GUGGUUCACGCCCCCGA 17 2128

HSV2-UL19-1889 + UCGAUGUUCAUGAGCGA 17 2129

HSV2-UL19-1095 - CUACGCAGCGCCCGCGA 17 1335

HSV2-UL19-1655 + GGUGCCCGUGGCCGCGA 17 1895

HSV2-UL19-1598 + GUUGGCCGUGGCCGCGA 17 1838

HSV2-UL19-1975 + CGAUGGCCUCGCGGCGA 17 2215

HSV2-UL19-920 - GGCGUGCAACGUGGCGA 17 1160

HSV2-UL19-907 - CUCGUGUGGGACUGCGA 17 1147

HSV2-UL19-1973 + CACGAGCCAGGCCUCGA 17 2213

HSV2-UL19-1884 + CGGAUUCGCGGCCUCGA 17 2124

HSV2-UL19-958 - CGCCAACACCGCGUCGA 17 1198

HSV2-UL19-949 - GGGCGCGUCGUCGUCGA 17 1189

HSV2-UL19-753 - CGCGGGCGGCCGGUCGA 17 993

HSV2-UL19-959 - AACAUGCGCAUCUUCGA 17 1199

HSV2-UL19-1060 - UACGCGGGGGACAAGGA 17 1300 HSV2-UL19-1995 + CUCAAACGCCCCCAGGA 17 2235

HSV2-UL19-1954 + GUCCUCCACCUUCAGGA 17 2194

HSV2-UL19-1588 + GAAGCAGGGGCUGAGGA 17 1828

HSV2-UL19-1969 + CGGGGCACGGCCACGGA 17 2209

HSV2-UL19-1831 + CUCGAACGCCCCGCGGA 17 2071

HSV2-UL19-1688 + CACCACGGGCUGGCGGA 17 1928

HSV2-UL19-1653 + CGUAACGGGGUUGCGGA 17 1893

HSV2-UL19-1627 + GCAGCCAAAGACCGGGA 17 1867

HSV2-UL19-1898 + AAAACAG CUGCCGGGGA 17 2138

HSV2-UL19-3087 + GCGCGCGAGCUGCUGGA 17 7470

HSV2-UL19-1716 + CG CAAACAG CG CG U G G A 17 1956

HSV2-UL19-1770 + GUAGAUUUUGUGGUGGA 17 2010

HSV2-UL19-1720 + GUAUUCGCCAUUUUGGA 17 1960

HSV2-UL19-1605 + GCCGUGGUCGUACAUGA 17 1845

HSV2-UL19-757 - CCUGCAGUCCUUCCUGA 17 997

HSV2-UL19-769 - CGUCACGGCGCUGGUGA 17 1009

HSV2-UL19-1957 + CAGGAGGCGCUGUUUGA 17 2197

HSV2-UL19-1794 + GGGCCGCGUGUCGCAUA 17 2034

HSV2-UL19-762 - GACGUGCCGGUGACCUA 17 1002

HSV2-UL19-1935 + GACCAGCCUGUCGCCUA 17 2175

HSV2-UL19-1833 + CCCGCGGACGGCGGCUA 17 2073

HSV2-UL19-1882 + GCGACGUACGCCCCGUA 17 2122

HSV2-UL19-824 - GAGGCCGCGAAUCCGUA 17 1064

HSV2-UL19-1071 - UCGCAGCGGUUCUCGUA 17 1311

HSV2-UL19-1928 + GCGCCCACCAGGGGGUA 17 2168

HSV2-UL19-1019 - CGUCCGCAACCCCGUUA 17 1259

HSV2-UL19-1003 - GUGGCCCGCCACGAAAC 17 1243

HSV2-UL19-1031 - CGGUCGUGGCGGGAAAC 17 1271

HSV2-UL19-1085 - CGUCUCAUCGUGGAAAC 17 1325

HSV2-UL19-1569 + GGUAGGCUUCCUGAAAC 17 1809

HSV2-UL19-948 - AGCGCAUGUUCCACAAC 17 1188

HSV2-UL19-730 - AACGAUAUCUCGACAAC 17 970

HSV2-UL19-1075 - GGGACCUGCUGUACAAC 17 1315

HSV2-UL19-1078 - GGGCCUAUCACCUCAAC 17 1318

HSV2-UL19-780 - AGCAACUCGAGGCGAAC 17 1020

HSV2-UL19-765 - GCGAGAUGGUCUUGAAC 17 1005

HSV2-UL19-1650 + UUGCCCAUGUCCGUAAC 17 1890

HSV2-UL19-1685 + CGGGCGUGCUGCACCAC 17 1925

HSV2-UL19-1043 - GCGCCGGCAUGGACCAC 17 1283

HSV2-UL19-1869 + GGCACUCGGCGACCCAC 17 2109

HSV2-UL19-1595 + ACCGCUGCGACG CCCAC 17 1835

HSV2-UL19-1841 + GCGUCACGAAACGCCAC 17 2081

HSV2-UL19-1018 - ACCGCCGUCGCGGCCAC 17 1258

HSV2-UL19-1679 + UGGUGAUACAGGGCCAC 17 1919

HSV2-UL19-1614 + UUGAUGUCCGUGGCCAC 17 1854

HSV2-UL19-1571 + CCUGAAACAGGCCGCAC 17 1811 HSV2-UL19-926 - CCGACGACCGGCCGCAC 17 1166

HSV2-UL19-1756 + GCUCGGGCACUCCUCAC 17 1996

HSV2-UL19-1950 + AUCUCGCCGUAGGUCAC 17 2190

HSV2-UL19-1753 + UGGGCGGGGUCGGUCAC 17 1993

HSV2-UL19-1624 + UGGGCGCAGCCAAAGAC 17 1864

HSV2-UL19-989 - UAUCACCAGCUCAAGAC 17 1229

HSV2-UL19-3101 + G CCAAU U U U ACAG AG AC 17 7484

HSV2-UL19-1787 + CACGCCGCCGCGUAGAC 17 2027

HSV2-UL19-925 - CCGACGCCGCCGACGAC 17 1165

HSV2-UL19-928 - CGCACCGGCCGGCCGAC 17 1168

HSV2-UL19-787 - UGGUGGCCAUAGGCGAC 17 1027

HSV2-UL19-1885 + GGAUUCGCGGCCUCGAC 17 2125

HSV2-UL19-1961 + ACCAG CACCCCG U CG AC 17 2201

HSV2-UL19-950 - GGCGCGUCGUCGUCGAC 17 1190

HSV2-UL19-754 - GCGGGCGGCCGGUCGAC 17 994

HSV2-UL19-960 - ACAUGCGCAUCUUCGAC 17 1200

HSV2-UL19-1589 + AAGCAGGGGCUGAGGAC 17 1829

HSV2-UL19-1850 + CUGGAUCGCCCCCGGAC 17 2090

HSV2-UL19-1717 + GCAAACAGCGCGUGGAC 17 1957

HSV2-UL19-1676 + UCUUGAGCUGGUGAUAC 17 1916

HSV2-UL19-878 - AGUGCAUCACCAGCUAC 17 1118

HSV2-UL19-825 - AGGCCGCGAAUCCGUAC 17 1065

HSV2-UL19-1072 - CGCAGCGGUUCUCGUAC 17 1312

HSV2-UL19-841 - GCCCGACAACGCCAACC 17 1081

HSV2-UL19-799 - CCUGGGGCUGUUCAACC 17 1039

HSV2-UL19-1892 + UGGACAGCCGCAGAACC 17 2132

HSV2-UL19-1670 + GACG G U G AACCCG AACC 17 1910

HSV2-UL19-781 - GCAACUCGAGGCGAACC 17 1021

HSV2-UL19-812 - U U G G G G AAAG G ACCACC 17 1052

HSV2-UL19-1923 + GGUCCAUGGCGCCCACC 17 2163

HSV2-UL19-855 - GGGCGAUCCAGGCCACC 17 1095

HSV2-UL19-806 - GCCCGCCCCCGGCCACC 17 1046

HSV2-UL19-1664 + UUUCGUGGCGGGCCACC 17 1904

HSV2-UL19-1814 + CCUCGACGUGGGCCACC 17 2054

HSV2-UL19-964 - CAUGGCCCCCCAGCACC 17 1204

HSV2-UL19-1622 + CGGCGCGCCGCGGCACC 17 1862

HSV2-UL19-1911 + GGCCGGGGGCGGGCACC 17 2151

HSV2-UL19-1757 + CUCGGGCACUCCUCACC 17 1997

HSV2-UL19-1625 + G G G CG CAG CCAAAG ACC 17 1865

HSV2-UL19-1064 - CCACGGCCAGAGCGACC 17 1304

HSV2-UL19-904 - UCACCUGAUGCGCGACC 17 1144

HSV2-UL19-970 - CCCGGCCCUGCGCGACC 17 1210

HSV2-UL19-1978 + GCUUCAGCUCGGCGACC 17 2218

HSV2-UL19-732 - ACGGGCGCCUGGCGACC 17 972

HSV2-UL19-1870 + CGGCGACCCACCGGACC 17 2110

HSV2-UL19-889 - GGCCGUGUAUCGGGACC 17 1129 HSV2-UL19-804 - CGCGCACGCCGGGGACC 17 1044

HSV2-UL19-1698 + CCAGGGCCGGGGGGACC 17 1938

HSV2-UL19-1012 - GCGCGGAAACGUGGACC 17 1252

HSV2-UL19-873 - CGAGGACCGCAGCUACC 17 1113

HSV2-UL19-1982 + GGUCGCCCGGGCAACCC 17 2222

HSV2-UL19-1671 + ACGGUGAACCCGAACCC 17 1911

HSV2-UL19-923 - GCUGCUGCACAACACCC 17 1163

HSV2-UL19-1657 + CGG UG U AGCCCACACCC 17 1897

HSV2-UL19-990 - AAGACGGGCCUCCACCC 17 1230

HSV2-UL19-1573 + ACGAGCUCGCGGCACCC 17 1813

HSV2-UL19-736 - GGUUGCCCGGGCGACCC 17 976

HSV2-UL19-991 - AGACGGGCCUCCACCCC 17 1231

HSV2-UL19-792 - CAACGUGCCCUACCCCC 17 1032

HSV2-UL19-1994 + CGCGCUCAAACGCCCCC 17 2234

HSV2-UL19-1691 + AGAAGUAGUUGGCCCCC 17 1931

HSV2-UL19-848 - CGGCGGCGAAGUCCCCC 17 1088

HSV2-UL19-971 - GGCGCGCGACGUCCCCC 17 1211

HSV2-UL19-972 - CGUCCCCCUGGUCCCCC 17 1212

HSV2-UL19-969 - CG CG CCCAACU U CCCCC 17 1209

HSV2-UL19-1917 + CCGGG UU GAACAGCCCC 17 2157

HSV2-UL19-805 - GACCUGGUGCCCGCCCC 17 1045

HSV2-UL19-942 - GUCCCGGAGAUCGCCCC 17 1182

HSV2-UL19-1849 + GUGGCCUGGAUCGCCCC 17 2089

HSV2-UL19-1840 + GCCGAGCUCCAGGCCCC 17 2080

HSV2-UL19-858 - CAACG G CAACCUG CCCC 17 1098

HSV2-UL19-796 - GACGUUCGUCCUGCCCC 17 1036

HSV2-UL19-728 - GGAGAAGGCGCCUCCCC 17 968

HSV2-UL19-862 - GGCGUUUCGUGACGCCC 17 1102

HSV2-UL19-1038 - GGUCUUUGGCUGCGCCC 17 1278

HSV2-UL19-876 - GCACGUGUUCUGCGCCC 17 1116

HSV2-UL19-1981 + GGCGACCAGGGUCGCCC 17 2221

HSV2-UL19-1839 + CGCCGAGCUCCAGGCCC 17 2079

HSV2-UL19-892 - GGCCCACGUCGAGGCCC 17 1132

HSV2-UL19-791 - GGUCUUCCUGGAGGCCC 17 1031

HSV2-UL19-973 - CCUGGUCCCCCCGGCCC 17 1213

HSV2-UL19-830 - GCGGCCCCGGCCGGCCC 17 1070

HSV2-UL19-909 - UAUGCGACACGCGGCCC 17 1149

HSV2-UL19-828 - GGCGUACGUCGCGGCCC 17 1068

HSV2-UL19-1976 + GAUGGCCCGCCUUGCCC 17 2216

HSV2-UL19-735 - GGCGACCAGGGUUGCCC 17 975

HSV2-UL19-807 - CCCGCGCGUUCCCUCCC 17 1047

HSV2-UL19-1912 + GGCGGGCACCAGGUCCC 17 2152

HSV2-UL19-1862 + GGUGCAGCUCCAGAGCC 17 2102

HSV2-UL19-1972 + GGUCAGGUCCACGAGCC 17 2212

HSV2-UL19-773 - CAAGGCCGUGCGGAGCC 17 1013

HSV2-UL19-832 - AGCGUU UUCUGAACGCC 17 1072 HSV2-UL19-802 - GCUUCGCCGCGCACGCC 17 1042

HSV2-UL19-861 - UGGCGU UUCGUGACGCC 17 1101

HSV2-UL19-1828 + GCAGGUAAAACACCGCC 17 2068

HSV2-UL19-1090 - CAGUUUAAGCGCCCGCC 17 1330

HSV2-UL19-1857 + GGGGGGACUUCGCCGCC 17 2097

HSV2-UL19-1874 + GGCGAGGCGCUGCCGCC 17 2114

HSV2-UL19-1842 + CCACCGGACACAGCGCC 17 2082

HSV2-UL19-1800 + GGGGCGGCAGCAGCGCC 17 2040

HSV2-UL19-1846 + GCCGUUGACCACGCGCC 17 2086

HSV2-UL19-1823 + GAGUCACGAGCCGCGCC 17 2063

HSV2-UL19-1601 + CCGCGAAGGGCCGCGCC 17 1841

HSV2-UL19-1876 + UGCUGCAUGUCCGCGCC 17 2116

HSV2-UL19-1702 + GGGGGACGUCGCGCGCC 17 1942

HSV2-UL19-731 - UCUCGACAACGGGCGCC 17 971

HSV2-UL19-1980 + CGGCGACCAGGGUCGCC 17 2220

HSV2-UL19-1983 + GGGCAACCCUGGUCGCC 17 2223

HSV2-UL19-745 - GCGAGGCCAUCGAGGCC 17 985

HSV2-UL19-836 - GCGCCUCGCCCACGGCC 17 1076

HSV2-UL19-1996 + ACGCCCCCAGGACGGCC 17 2236

HSV2-UL19-1852 + UCGCCCCCGGACCGGCC 17 2092

HSV2-UL19-1880 + CCGCGCCGGGGCCGGCC 17 2120

HSV2-UL19-1694 + AGUUGGCCCCCAGGGCC 17 1934

HSV2-UL19-1988 + UGGGCAACAGCAGGGCC 17 2228

HSV2-UL19-1706 + CCAGGUCGCG CAG G G CC 17 1946

HSV2-UL19-897 - CUUUACCCUGCCGGGCC 17 1137

HSV2-UL19-1805 + CCGCCCAGCUCCGGGCC 17 2045

HSV2-UL19-1781 + CGUCGGCCGCGCGGGCC 17 2021

HSV2-UL19-864 - UCGUGACGCCCGGGGCC 17 1104

HSV2-UL19-1810 + CGUCCACCAGCUGGGCC 17 2050

HSV2-UL19-1916 + CGAAGCGCUCCAUGGCC 17 2156

HSV2-UL19-1848 + CCACGCGCCAGGUGGCC 17 2088

HSV2-UL19-1906 + CGGGGCUCCGGGUGGCC 17 2146

HSV2-UL19-896 - GACGACU U U ACCCUGCC 17 1136

HSV2-UL19-1895 + CCAAAAAAACAGCUGCC 17 2135

HSV2-UL19-1082 - GGCCAAACAUCGCUGCC 17 1322

HSV2-UL19-734 - UGGCGACCAGGGUUGCC 17 974

HSV2-UL19-854 - CGGUCCGGGGGCGAUCC 17 1094

HSV2-UL19-1036 - GCCGGCCCAGCCCCUCC 17 1276

HSV2-UL19-1934 + GUCUCUCCAGGGCCUCC 17 2174

HSV2-UL19-1804 + CCUGCCCGCCCAGCUCC 17 2044

HSV2-UL19-1000 - CUUUCUGGGCCAGCUCC 17 1240

HSV2-UL19-1838 + GGCCAACGCCGAGCUCC 17 2078

HSV2-UL19-1580 + CCACGAUGAGACGCUCC 17 1820

HSV2-UL19-1903 + GGAACGCGCGGGGCUCC 17 2143

HSV2-UL19-1761 + CACCGGGGGCGAUCUCC 17 2001

HSV2-UL19-1932 + CGUAGAUGCGUCUCUCC 17 2172 HSV2-UL19-1941 + G U U CAU CCAGCG U CU CC 17 2181

HSV2-UL19-3071 - GCUCGCGCGCAACGUCC 17 7454

HSV2-UL19-3078 - CAACG UCCAGGCCGUCC 17 7461

HSV2-UL19-1945 + GGCGGCCCACGUCGUCC 17 2185

HSV2-UL19-851 - GUCCCCCCGGCCGGUCC 17 1091

HSV2-UL19-1792 + AGUCUCGGUGGCGGUCC 17 2032

HSV2-UL19-941 - GCAGAACAUGGUGGUCC 17 1181

HSV2-UL19-1722 + UUUGGAUGGUGUGGUCC 17 1962

HSV2-UL19-846 - GCCGACGUCGAGCUUCC 17 1086

HSV2-UL19-789 - CGACAGGCUGGUCUUCC 17 1029

HSV2-UL19-738 - CGACACGAGCUUCUUCC 17 978

HSV2-UL19-1584 + GGUGAAGAACUUGAAGC 17 1824

HSV2-UL19-1986 + GUUGCAUGGGCAACAGC 17 2226

HSV2-UL19-1593 + AGGCCCCGUUGUACAGC 17 1833

HSV2-UL19-1808 + UAAAGUCGUCCACCAGC 17 2048

HSV2-UL19-1632 + GCUGGGCCGGCCCCAGC 17 1872

HSV2-UL19-893 - CGAGGCCCUGGCCCAGC 17 1133

HSV2-UL19-1942 + CCUCCUGCAUAUCCAGC 17 2182

HSV2-UL19-1783 + GGGUGUUGUGCAGCAGC 17 2023

HSV2-UL19-1637 + CCGCGUUGUCUAGCAGC 17 1877

HSV2-UL19-134 + GCUGGAUGGCGCGCAGC 17 535

HSV2-UL19-1745 + GAUUGGCGGGAUGCAGC 17 1985

HSV2-UL19-1826 + GAAUCGCGGCUUGCAGC 17 2066

HSV2-UL19-898 - CCUGCCGGGCCCGGAGC 17 1138

HSV2-UL19-1665 + GGCGGGCCACCUGGAGC 17 1905

HSV2-UL19-1675 + GGAGGCCCGUCUUGAGC 17 1915

HSV2-UL19-1822 + CGUGUUGUUCCAGUAGC 17 2062

HSV2-UL19-1559 + CGCGGGCGCUGCGUAGC 17 1799

HSV2-UL19-1834 + ACGGCGGCUAUGGUAGC 17 2074

HSV2-UL19-1550 + GAGACAGGCCCUUUAGC 17 1790

HSV2-UL19-1860 + GCGAAGAAGUCGAACGC 17 2100

HSV2-UL19-1728 + GGCCAUGAGCAGCACGC 17 1968

HSV2-UL19-801 - CGCUUCGCCGCGCACGC 17 1041

HSV2-UL19-962 - GACGGGGCGCUGCACGC 17 1202

HSV2-UL19-782 - GGCGAACCGGGAGACGC 17 1022

HSV2-UL19-957 - UCCGCGGCGCCCGACGC 17 1197

HSV2-UL19-912 - GACUGCCGGAUUGACGC 17 1152

HSV2-UL19-1055 - GCCGGCGGCGUGUACGC 17 1295

HSV2-UL19-1827 + AGCAGGUAAAACACCGC 17 2067

HSV2-UL19-936 - GGGCGCUGCUGCACCGC 17 1176

HSV2-UL19-1049 - CCGGCCCUGCAACCCGC 17 1289

HSV2-UL19-1608 + CGGCCGCGCGUCCCCGC 17 1848

HSV2-UL19-1089 - GCAGUUUAAGCGCCCGC 17 1329

HSV2-UL19-1606 + CCCCGCGUACACGCCGC 17 1846

HSV2-UL19-1856 + CGGGGGGACUUCGCCGC 17 2096

HSV2-UL19-1024 - ACUUUUACCUCGGCCGC 17 1264 HSV2-UL19-870 - CCAUAGCCGCCGUCCGC 17 1110

HSV2-UL19-1799 + AGGGGCGGCAGCAGCGC 17 2039

HSV2-UL19-1900 + CCGGGGAGGGAACGCGC 17 2140

HSV2-UL19-750 - CGCACGCCGACACGCGC 17 990

HSV2-UL19-1780 + GGCGGCGUCGGCCGCGC 17 2020

HSV2-UL19-1600 + GCCGCGAAGGGCCGCGC 17 1840

HSV2-UL19-1875 + CUGCUGCAUGUCCGCGC 17 2115

HSV2-UL19-1040 - CAGGUGCCGCGGCGCGC 17 1280

HSV2-UL19-1703 + CGCGCGCCAGGUCGCGC 17 1943

HSV2-UL19-768 - CAACCUCGUCACGGCGC 17 1008

HSV2-UL19-868 - CCGCCACGCCAUGGCGC 17 1108

HSV2-UL19-1565 + GGCGGGGUCGCUGGCGC 17 1805

HSV2-UL19-1633 + CCACGACCGCG U UGCGC 17 1873

HSV2-UL19-1558 + GUGGGCCUCCCCAUCGC 17 1798

HSV2-UL19-880 - CGUGAACGACUACUCGC 17 1120

HSV2-UL19-1683 + CCCCGCCGCGCUCUCGC 17 1923

HSV2-UL19-932 - GGUGCCGGCCUUCUCGC 17 1172

HSV2-UL19-1576 + CUUAAACUGCACGUCGC 17 1816

HSV2-UL19-1564 + UAGCAGGGCGGGGUCGC 17 1804

HSV2-UL19-844 - GCGUUCGACUUCUUCGC 17 1084

HSV2-UL19-1047 - ACAUCAACUACUUUCGC 17 1287

HSV2-UL19-742 - UUCUUCCUGGGCAAGGC 17 982

HSV2-UL19-1767 + CGCCCCCGCGAGAAGGC 17 2007

HSV2-UL19-788 - GGCCAUAGGCGACAGGC 17 1028

HSV2-UL19-1965 + GUCGGCGUGCGUCAGGC 17 2205

HSV2-UL19-835 - AGCGCCUCGCCCACGGC 17 1075

HSV2-UL19-922 - UUAACCGCAACGACGGC 17 1162

HSV2-UL19-1032 - CGUGGCGGGAAACCGGC 17 1272

HSV2-UL19-927 - CGACCGGCCGCACCGGC 17 1167

HSV2-UL19-1851 + AUCGCCCCCGGACCGGC 17 2091

HSV2-UL19-1575 + GCUCGCGGCACCCCGGC 17 1815

HSV2-UL19-849 - GGCGAAGUCCCCCCGGC 17 1089

HSV2-UL19-829 - UACGUCGCGGCCCCGGC 17 1069

HSV2-UL19-1806 + CCAGCUCCGGGCCCGGC 17 2046

HSV2-UL19-1773 + CGGUCCAGUCGGCCGGC 17 2013

HSV2-UL19-1879 + UCCGCGCCGGGGCCGGC 17 2119

HSV2-UL19-980 - GCCCGCGAGAGCGCGGC 17 1220

HSV2-UL19-1052 - CCGCGGGGACGCGCGGC 17 1292

HSV2-UL19-1659 + CCACGUUUCCGCGCGGC 17 1899

HSV2-UL19-752 - CGACACGCGCGGGCGGC 17 992

HSV2-UL19-995 - GUUCACCGUCGUGCGGC 17 1235

HSV2-UL19-1775 + CGGCCGGCCGGUGCGGC 17 2015

HSV2-UL19-1772 + UGGACGGUCCAGUCGGC 17 2012

HSV2-UL19-1693 + UAGUUGGCCCCCAGGGC 17 1933

HSV2-UL19-1562 + GCGCUGCGUAGCAGGGC 17 1802

HSV2-UL19-1705 + GCCAGGUCGCGCAGGGC 17 1945 HSV2-UL19-1611 + CCCGCGGGUUGCAGGGC 17 1851

HSV2-UL19-1005 - CCCGCCACGAAACGGGC 17 1245

HSV2-UL19-1686 + CGUGCUGCACCACGGGC 17 1926

HSV2-UL19-1044 - CGGCAUGGACCACGGGC 17 1284

HSV2-UL19-1718 + ACAGCGCGUGGACGGGC 17 1958

HSV2-UL19-902 - CCCGGAGCUGGGCGGGC 17 1142

HSV2-UL19-1920 + UGAACAGCCCCAGGGGC 17 2160

HSV2-UL19-1845 + GACACAGCGCCAGGGGC 17 2085

HSV2-UL19-1629 + CAAAGACCGGGAGGGGC 17 1869

HSV2-UL19-1878 + CAUGUCCGCGCCGGGGC 17 2118

HSV2-UL19-1910 + UCCGGGUGGCCGGGGGC 17 2150

HSV2-UL19-1035 - GGGAAACCGGCUGGGGC 17 1275

HSV2-UL19-901 - CGGGCCCGGAGCUGGGC 17 1141

HSV2-UL19-1631 + ACCGGGAGGGGCUGGGC 17 1871

HSV2-UL19-1749 + GGAUGCAGCGGGUGGGC 17 1989

HSV2-UL19-1915 + GCGAAGCGCUCCAUGGC 17 2155

HSV2-UL19-1738 + UGCAGCGUGAGCAUGGC 17 1978

HSV2-UL19-985 - ACCUACGCGCUCAUGGC 17 1225

HSV2-UL19-1030 - CGCAACGCGGUCGUGGC 17 1270

HSV2-UL19-1663 + CCCGCCCGUUUCGUGGC 17 1903

HSV2-UL19-1905 + GCGGGGCUCCGGGUGGC 17 2145

HSV2-UL19-1743 + UUGGCCACCAGAUUGGC 17 1983

HSV2-UL19-1620 + CUGCCCGUGGUCCAUGC 17 1860

HSV2-UL19-777 - CCACCUGCUGGAUAUGC 17 1017

HSV2-UL19-910 - ACCGCCACCGAGACUGC 17 1150

HSV2-UL19-1955 + CCUUCAGGAAGGACUGC 17 2195

HSV2-UL19-776 - CGUGGGCCGCCACCUGC 17 1016

HSV2-UL19-895 - GGACGACUUUACCCUGC 17 1135

HSV2-UL19-1894 + CCCAAAAAAACAGCUGC 17 2134

HSV2-UL19-3079 + CGUUGCGCGCGAGCUGC 17 7462

HSV2-UL19-951 - CGCCAUGCUCACGCUGC 17 1191

HSV2-UL19-761 - CACCGAGGCCGACGUGC 17 1001

HSV2-UL19-929 - AAUCUACUAUUACGUGC 17 1169

HSV2-UL19-1723 + UGGUGUGGUCCAGGUGC 17 1963

HSV2-UL19-952 - GCUCACGCUGCAGGUGC 17 1192

HSV2-UL19-756 - GCCGGUCGACGGGGUGC 17 996

HSV2-UL19-930 - CUAUUACGUGCUGGUGC 17 1170

HSV2-UL19-1609 + GCGUCCCCGCGGGUUGC 17 1849

HSV2-UL19-967 - GUCCACGCGCUGUUUGC 17 1207

HSV2-UL19-944 - GCUGCAUCCCGCCAAUC 17 1184

HSV2-UL19-1788 + GCCCCCCCGCGUCAAUC 17 2028

HSV2-UL19-1992 + ACAGCACGUGCAGCAUC 17 2232

HSV2-UL19-1801 + GCGCCGGGUCGCGCAUC 17 2041

HSV2-UL19-1867 + ACUGCUCCGCGGUCAUC 17 2107

HSV2-UL19-784 - GCGCGUGCGCGCGGAUC 17 1024

HSV2-UL19-1567 + CGCUGGCGCAGGUGAUC 17 1807 HSV2-UL19-888 - GUGCAUGGCCGUGUAUC 17 1128

HSV2-UL19-882 - ACAUCGUGACCUACCUC 17 1122

HSV2-UL19-1817 + ACGGCCAUGCACUCCUC 17 2057

HSV2-UL19-1803 + GCCUGCCCGCCCAGCUC 17 2043

HSV2-UL19-1755 + CGGUCACGGGAUCGCUC 17 1995

HSV2-UL19-1602 + GCCGCGCCGGGUCGCUC 17 1842

HSV2-UL19-903 - GCUGGGCGGGCAGGCUC 17 1143

HSV2-UL19-1902 + GGGAACGCGCGGGGCUC 17 2142

HSV2-UL19-842 - CAACGCCAACCUGGCUC 17 1082

HSV2-UL19-1760 + UCACCGGGGGCGAUCUC 17 2000

HSV2-UL19-1921 + GGGGCAGGACGAACGUC 17 2161

HSV2-UL19-820 - ACAUCGACGCGGCCGUC 17 1060

HSV2-UL19-1733 + GUGUUGGCGCCCGCGUC 17 1973

HSV2-UL19-1963 + GCGUGUCGGCGUGCGUC 17 2203

HSV2-UL19-850 - AGUCCCCCCGGCCGGUC 17 1090

HSV2-UL19-817 - CCAUGGAGAACGCGGUC 17 1057

HSV2-UL19-837 - UCGCCCACGGCCGGGUC 17 1077

HSV2-UL19-1971 + GCUGCGUCGCCGUGGUC 17 2211

HSV2-UL19-859 - GCCCCUGGCGCUGUGUC 17 1099

HSV2-UL19-1936 + CGCGCACGCGCGUUGUC 17 2176

HSV2-UL19-1865 + GCCAGGUUGGCGUUGUC 17 2105

HSV2-UL19-1953 + CGGUGUCCUCCACCUUC 17 2193

HSV2-UL19-993 - GCCUCCACCCCGGGUUC 17 1233

HSV2-UL19-998 - GUCGGAGGCGUACUUUC 17 1238

HSV2-UL19-1094 - CCCGUGCGGCCUGUUUC 17 1334

HSV2-UL19-1553 + UGAGAUACUGCGUAAAG 17 1793

HSV2-UL19-1668 + CGUUCUCGGUCACGAAG 17 1908

HSV2-UL19-1764 + CGUACACGCGGUCGAAG 17 2004

HSV2-UL19-737 - UGGUCGCCGAGCUGAAG 17 977

HSV2-UL19-1925 + U CCAU G G CG CCCACCAG 17 2165

HSV2-UL19-1700 + AGGGCCGGGGGGACCAG 17 1940

HSV2-UL19-1919 + GGGUUGAACAGCCCCAG 17 2159

HSV2-UL19-1844 + ACCGGACACAGCGCCAG 17 2084

HSV2-UL19-1990 + G G CAACAG CAG G G CCAG 17 2230

HSV2-UL19-1586 + UGAAGAACUUGAAGCAG 17 1826

HSV2-UL19-1636 + GCCGCGUUGUCUAGCAG 17 1876

HSV2-UL19-1070 - ACCCGUGGGCGUCGCAG 17 1310

HSV2-UL19-1577 + CUGCACGUCGCUGGCAG 17 1817

HSV2-UL19-1744 + AGAUUGGCGGGAUGCAG 17 1984

HSV2-UL19-1797 + U CGCAG U CCCACACG AG 17 2037

HSV2-UL19-1061 - ACGCGGGGGACAAGGAG 17 1301

HSV2-UL19-1628 + CAGCCAAAGACCGGGAG 17 1868

HSV2-UL19-1549 + AGAGACAGGCCCUUUAG 17 1789

HSV2-UL19-1011 - CCAGCCGCGCGGAAACG 17 1251

HSV2-UL19-1026 - CCCGCUGCUAGACAACG 17 1266

HSV2-UL19-1076 - GGACCUGCUGUACAACG 17 1316 HSV2-UL19-1027 - CGUGUACCUGCGCAACG 17 1267

HSV2-UL19-919 - CGCGGCGGCGUGCAACG 17 1159

HSV2-UL19-1079 - GGCCUAUCACCUCAACG 17 1319

HSV2-UL19-815 - GCUGUCCAUGGAGAACG 17 1055

HSV2-UL19-1859 + CGCGAAGAAGUCGAACG 17 2099

HSV2-UL19-766 - CGAGAUGGUCUUGAACG 17 1006

HSV2-UL19-1651 + UGCCCAUGUCCGUAACG 17 1891

HSV2-UL19-1795 + CG U CG CAG U CCCACACG 17 2035

HSV2-UL19-908 - CGGCCUUAUGCGACACG 17 1148

HSV2-UL19-1763 + GCAGCGUGGCGUACACG 17 2003

HSV2-UL19-968 - UGCGGGCGCCGACCACG 17 1208

HSV2-UL19-1680 + GGUGAUACAGGGCCACG 17 1920

HSV2-UL19-1615 + UGAUGUCCGUGGCCACG 17 1855

HSV2-UL19-1713 + UGGGCGCGUUGGCCACG 17 1953

HSV2-UL19-774 - GCGGAGCCUGGACGACG 17 1014

HSV2-UL19-956 - CUCCGCGGCGCCCGACG 17 1196

HSV2-UL19-818 - GCUCAUGAACAUCGACG 17 1058

HSV2-UL19-1886 + GAUUCGCGGCCUCGACG 17 2126

HSV2-UL19-1812 + GGGCCAGGGCCUCGACG 17 2052

HSV2-UL19-1730 + CAUGUUGGCCGUCGACG 17 1970

HSV2-UL19-755 - CGGGCGGCCGGUCGACG 17 995

HSV2-UL19-961 - CAUGCGCAUCUUCGACG 17 1201

HSV2-UL19-1947 + UCACCAGCGCCGUGACG 17 2187

HSV2-UL19-911 - AGACUGCCGGAUUGACG 17 1151

HSV2-UL19-917 - GCACGAGCCCGUCUACG 17 1157

HSV2-UL19-826 - GGCCGCGAAUCCGUACG 17 1066

HSV2-UL19-1073 - GCAGCGGUUCUCGUACG 17 1313

HSV2-UL19-1054 - GGCCGGCGGCGUGUACG 17 1294

HSV2-UL19-760 - GAAGGUGGAGGACACCG 17 1000

HSV2-UL19-935 - GGGGCGCUGCUGCACCG 17 1175

HSV2-UL19-1758 + UCGGGCACUCCUCACCG 17 1998

HSV2-UL19-1080 - CUUCAAGU U CU U CACCG 17 1320

HSV2-UL19-840 - CGAGUGCCAGAUGACCG 17 1080

HSV2-UL19-1068 - CGGCCACGGCCAACCCG 17 1308

HSV2-UL19-1048 - GCCGGCCCUGCAACCCG 17 1288

HSV2-UL19-1672 + CGG UG AACCCGAACCCG 17 1912

HSV2-UL19-1045 - GUUCAUCGCCACCCCCG 17 1285

HSV2-UL19-1765 + GUCGAAGCGGACCCCCG 17 2005

HSV2-UL19-1820 + CGGGGAGGUCGCCCCCG 17 2060

HSV2-UL19-987 - CUUCAAGAUGAGCCCCG 17 1227

HSV2-UL19-1830 + GGUCCUCGAACGCCCCG 17 2070

HSV2-UL19-1642 + GCAGCGGGGGGGCCCCG 17 1882

HSV2-UL19-885 - CGGGGGCGACCUCCCCG 17 1125

HSV2-UL19-1607 + CCGGCCGCGCGUCCCCG 17 1847

HSV2-UL19-977 - CUCCAUCCGCCAGCCCG 17 1217

HSV2-UL19-863 - GCGUUUCGUGACGCCCG 17 1103 HSV2-UL19-1619 + ACACGGCAUCCUGCCCG 17 1859

HSV2-UL19-1654 + GUUGCGGACGGUGCCCG 17 1894

HSV2-UL19-803 - CUUCGCCGCGCACGCCG 17 1043

HSV2-UL19-1091 - AG U U UAAGCGCCCGCCG 17 1331

HSV2-UL19-1877 + GCUGCAUGUCCGCGCCG 17 2117

HSV2-UL19-1621 + UCCAUGCCGGCGCGCCG 17 1861

HSV2-UL19-1734 + GCCCGCGUCGGGCGCCG 17 1974

HSV2-UL19-1970 + GGACGGCUGCGUCGCCG 17 2210

HSV2-UL19-1853 + CGCCCCCGGACCGGCCG 17 2093

HSV2-UL19-1871 + CCCACCGGACCCGGCCG 17 2111

HSV2-UL19-1881 + CGCGCCGGGGCCGGCCG 17 2121

HSV2-UL19-1643 + GGGGGGCCCCGCGGCCG 17 1883

HSV2-UL19-1023 - AACUUUUACCUCGGCCG 17 1263

HSV2-UL19-1583 + GGCCGUGAUGUCGGCCG 17 1823

HSV2-UL19-1695 + GUUGGCCCCCAGGGCCG 17 1935

HSV2-UL19-1707 + CAGGUCGCGCAGGGCCG 17 1947

HSV2-UL19-1603 + CCGGGUCGCUCUGGCCG 17 1843

HSV2-UL19-1579 + GCUGGCAGCGGUGGCCG 17 1819

HSV2-UL19-1907 + GGGGCUCCGGGUGGCCG 17 2147

HSV2-UL19-1597 + CGCCCACGGGUUGGCCG 17 1837

HSV2-UL19-1896 + CAAAAAAACAGCUGCCG 17 2136

HSV2-UL19-1039 - GCUGCGCCCAGGUGCCG 17 1279

HSV2-UL19-1866 + CUGCAUGAACUGCUCCG 17 2106

HSV2-UL19-955 - GGCGCUGCUGUGCUCCG 17 1195

HSV2-UL19-748 - GGCGACGCAGCCGUCCG 17 988

HSV2-UL19-869 - ACCAUAGCCGCCGUCCG 17 1109

HSV2-UL19-852 - UCCCCCCGGCCGGUCCG 17 1092

HSV2-UL19-1612 + AAAG U AG U U G AU G U CCG 17 1852

HSV2-UL19-1638 + CGCGUUGUCUAGCAGCG 17 1878

HSV2-UL19-1762 + CACCAUGUUCUGCAGCG 17 2002

HSV2-UL19-978 - GCACGCCCGCGAGAGCG 17 1218

HSV2-UL19-724 - CUGGGGGCGUUUGAGCG 17 964

HSV2-UL19-1551 + AGACAGGCCCUUUAGCG 17 1791

HSV2-UL19-1861 + CGAAGAAG U CGAACG CG 17 2101

HSV2-UL19-1899 + GCCGGGGAGGGAACGCG 17 2139

HSV2-UL19-749 - ACGCACGCCGACACGCG 17 989

HSV2-UL19-1051 - CAACCCGCGGGGACGCG 17 1291

HSV2-UL19-913 - ACUGCCGGAUUGACGCG 17 1153

HSV2-UL19-1056 - CCGGCGGCGUGUACGCG 17 1296

HSV2-UL19-937 - GGCGCUGCUGCACCGCG 17 1177

HSV2-UL19-1050 - CGGCCCUGCAACCCGCG 17 1290

HSV2-UL19-924 - CAACACCCAGGCCCGCG 17 1164

HSV2-UL19-1010 - ACGCUCACCCAGCCGCG 17 1250

HSV2-UL19-743 - GGCGGGCCAUCGCCGCG 17 983

HSV2-UL19-1025 - CUUUUACCUCGGCCGCG 17 1265

HSV2-UL19-1779 + CGGCGGCGUCGGCCGCG 17 2019 HSV2-UL19-871 - CAUAGCCGCCGUCCGCG 17 1111

HSV2-UL19-1658 + AGGUCCACGUUUCCGCG 17 1898

HSV2-UL19-1715 + CGCCCGCAAACAGCGCG 17 1955

HSV2-UL19-1901 + CGGGGAGGGAACGCGCG 17 2141

HSV2-UL19-1555 + AGUGGGUCUCUCGCGCG 17 1795

HSV2-UL19-856 - CCAGGCCACCUGGCGCG 17 1096

HSV2-UL19-783 - GACAACG CG CG U G CG CG 17 1023

HSV2-UL19-933 - GUGCCGGCCUUCUCGCG 17 1173

HSV2-UL19-1966 + UCGGCGUGCGUCAGGCG 17 2206

HSV2-UL19-1065 - GCCAGAGCGACCCGGCG 17 1305

HSV2-UL19-831 - CCCGGCCGGCCCCGGCG 17 1071

HSV2-UL19-981 - CCCGCGAGAGCGCGGCG 17 1221

HSV2-UL19-1563 + CGCUGCGUAGCAGGGCG 17 1803

HSV2-UL19-1006 - CCGCCACGAAACGGGCG 17 1246

HSV2-UL19-1590 + GGGGCUGAGGACGGGCG 17 1830

HSV2-UL19-845 - CGACUUCUUCGCGGGCG 17 1085

HSV2-UL19-1873 + GGACCCGGCCGUGGGCG 17 2113

HSV2-UL19-1750 + GAUGCAGCGGGUGGGCG 17 1990

HSV2-UL19-1836 + UAGCCGGCGCCAUGGCG 17 2076

HSV2-UL19-986 - CCUACGCGCUCAUGGCG 17 1226

HSV2-UL19-877 - UGUUCUGCGCCCUGGCG 17 1117

HSV2-UL19-879 - GAACAACACGCGAUGCG 17 1119

HSV2-UL19-1913 + CAGGUCCCCGGCGUGCG 17 2153

HSV2-UL19-1825 + CUCGUUGCCGUGAAUCG 17 2065

HSV2-UL19-1557 + CGUGGGCCUCCCCAUCG 17 1797

HSV2-UL19-744 - UCGCCGCGAGGCCAUCG 17 984

HSV2-UL19-1083 - CCUGGAGCGUCUCAUCG 17 1323

HSV2-UL19-779 - GCAGGAGGAGCAACUCG 17 1019

HSV2-UL19-883 - CAUCGUGACCUACCUCG 17 1123

HSV2-UL19-1974 + AGGCCUCGAUGGCCUCG 17 2214

HSV2-UL19-1818 + CGGCCAUGCACUCCUCG 17 2058

HSV2-UL19-1572 + GGUCUUCCACGAGCUCG 17 1812

HSV2-UL19-1092 - GGGGUGCCGCGAGCUCG 17 1332

HSV2-UL19-746 - CAUCGAGGCCUGGCUCG 17 986

HSV2-UL19-1682 + CCCCCGCCGCGCUCUCG 17 1922

HSV2-UL19-931 - UGGUGCCGGCCUUCUCG 17 1171

HSV2-UL19-891 - CCUGGUGGCCCACGUCG 17 1131

HSV2-UL19-827 - GUACGGGGCGUACGUCG 17 1067

HSV2-UL19-823 - G AACCACGACCCCG UCG 17 1063

HSV2-UL19-1016 - GGGCUACACCGCCGUCG 17 1256

HSV2-UL19-821 - CAUCGACGCGGCCGUCG 17 1061

HSV2-UL19-1028 - CCUGCGCAACGCGGUCG 17 1268

HSV2-UL19-1887 + CGGCCUCGACGGGGUCG 17 2127

HSV2-UL19-1883 + CGCCCCGUACGGAUUCG 17 2123

HSV2-UL19-1066 - CCCGGCGCGGCCCUUCG 17 1306

HSV2-UL19-843 - CGCGUUCGACUUCUUCG 17 1083 HSV2-UL19-872 - CGUCCGCGGGGCGUUCG 17 1112

HSV2-UL19-1661 + CCCCCCCGCCCGUUUCG 17 1901

HSV2-UL19-1059 - GUACGCGGGGGACAAGG 17 1299

HSV2-UL19-741 - CUUCUUCCUGGGCAAGG 17 981

HSV2-UL19-758 - GCAGUCCUUCCUGAAGG 17 998

HSV2-UL19-1926 + CCAUGGCGCCCACCAGG 17 2166

HSV2-UL19-1701 + GGGCCGGGGGGACCAGG 17 1941

HSV2-UL19-1847 + GUUGACCACGCGCCAGG 17 2087

HSV2-UL19-1001 - UCUGGGCCAGCUCCAGG 17 1241

HSV2-UL19-1943 + CCUGCAUAUCCAGCAGG 17 2183

HSV2-UL19-778 - CCUGCUGGAUAUGCAGG 17 1018

HSV2-UL19-1956 + UCAGGAAGGACUGCAGG 17 2196

HSV2-UL19-1964 + UGUCGGCGUGCGUCAGG 17 2204

HSV2-UL19-1062 - CGCGGGGGACAAGGAGG 17 1302

HSV2-UL19-1635 + GUUGCGCAGGUACACGG 17 1875

HSV2-UL19-1616 + GAUGUCCGUGGCCACGG 17 1856

HSV2-UL19-1656 + GCCCGUGGCCGCGACGG 17 1896

HSV2-UL19-1832 + GAACGCCCCGCGGACGG 17 2072

HSV2-UL19-1759 + CGGGCACUCCUCACCGG 17 1999

HSV2-UL19-874 - GGACCGCAGCUACCCGG 17 1114

HSV2-UL19-1574 + AGCUCGCGGCACCCCGG 17 1814

HSV2-UL19-847 - GACGUCGAGCUUCCCGG 17 1087

HSV2-UL19-1854 + GCCCCCGGACCGGCCGG 17 2094

HSV2-UL19-1053 - CGGGGACGCGCGGCCGG 17 1293

HSV2-UL19-1696 + UUGGCCCCCAGGGCCGG 17 1936

HSV2-UL19-1708 + AGGUCGCGCAGGGCCGG 17 1948

HSV2-UL19-1908 + GGGCUCCGGGUGGCCGG 17 2148

HSV2-UL19-853 - CCCCCCGGCCGGUCCGG 17 1093

HSV2-UL19-838 - CCCACGGCCGGGUCCGG 17 1078

HSV2-UL19-860 - CCUGGCGCUGUGUCCGG 17 1100

HSV2-UL19-1639 + GCGUUGUCUAGCAGCGG 17 1879

HSV2-UL19-1578 + CACGUCGCUGGCAGCGG 17 1818

HSV2-UL19-914 - CUGCCGGAUUGACGCGG 17 1154

HSV2-UL19-918 - CGAGCCCGUCUACGCGG 17 1158

HSV2-UL19-1057 - CGGCGGCGUGUACGCGG 17 1297

HSV2-UL19-938 - GCGCUGCUGCACCGCGG 17 1178

HSV2-UL19-979 - CGCCCGCGAGAGCGCGG 17 1219

HSV2-UL19-934 - UGCCGGCCUUCUCGCGG 17 1174

HSV2-UL19-982 - CCGCGAGAGCGCGGCGG 17 1222

HSV2-UL19-1960 + CUGUUUGAUGGCGGCGG 17 2200

HSV2-UL19-1007 - CGCCACGAAACGGGCGG 17 1247

HSV2-UL19-1959 + GCGCUGUUUGAUGGCGG 17 2199

HSV2-UL19-1087 - CGUGGAAACGGGAUCGG 17 1327

HSV2-UL19-884 - AUCGUGACCUACCUCGG 17 1124

HSV2-UL19-1790 + CAAUCCGGCAGUCUCGG 17 2030

HSV2-UL19-822 - AUCGACGCGGCCGUCGG 17 1062 HSV2-UL19-997 - UUCCGAGCGCGCGUCGG 17 1237

HSV2-UL19-1777 + GUGCGGCCGGUCGUCGG 17 2017

HSV2-UL19-1561 + GGCGCUGCGUAGCAGGG 17 1801

HSV2-UL19-1004 - GCCCGCCACGAAACGGG 17 1244

HSV2-UL19-1626 + CGCAGCCAAAGACCGGG 17 1866

HSV2-UL19-1855 + CCCCCGGACCGGCCGGG 17 2095

HSV2-UL19-1697 + UGGCCCCCAGGGCCGGG 17 1937

HSV2-UL19-1709 + GGUCGCGCAGGGCCGGG 17 1949

HSV2-UL19-1904 + ACGCGCGGGGCUCCGGG 17 2144

HSV2-UL19-1640 + CGUUGUCUAGCAGCGGG 17 1880

HSV2-UL19-1746 + UGGCGGGAUGCAGCGGG 17 1986

HSV2-UL19-915 - UGCCGGAUUGACGCGGG 17 1155

HSV2-UL19-751 - ACGCCGACACGCGCGGG 17 991

HSV2-UL19-1008 - GCCACGAAACGGGCGGG 17 1248

HSV2-UL19-1991 + AACAGCAGGGCCAGGGG 17 2231

HSV2-UL19-1798 + CAG U CCCACACG AG GG G 17 2038

HSV2-UL19-1673 + U G AACCCG AACCCG GG G 17 1913

HSV2-UL19-1897 + AAAAACAG CU G CCG G G G 17 2137

HSV2-UL19-1641 + GUUGUCUAGCAGCGGGG 17 1881

HSV2-UL19-1552 + CAGGCCCUUUAGCGGGG 17 1792

HSV2-UL19-916 - GCCGGAUUGACGCGGGG 17 1156

HSV2-UL19-1009 - CCACGAAACGGGCGGGG 17 1249

HSV2-UL19-1819 + CCAUGCACUCCUCGGGG 17 2059

HSV2-UL19-1617 + GUCCGUGGCCACGGGGG 17 1857

HSV2-UL19-1909 + CUCCGGGUGGCCGGGGG 17 2149

HSV2-UL19-1648 + GGUAAAAGUUUUGGGGG 17 1888

HSV2-UL19-1098 - CAGCGCCCGCGAUGGGG 17 1338

HSV2-UL19-900 - CCGGGCCCGGAGCUGGG 17 1140

HSV2-UL19-1727 + GUGGUCCAGGUGCUGGG 17 1967

HSV2-UL19-1940 + CACGCGCGUUGUCUGGG 17 2180

HSV2-UL19-1748 + GGGAUGCAGCGGGUGGG 17 1988

HSV2-UL19-940 - CACGCUGCAGAACAUGG 17 1180

HSV2-UL19-1737 + CUGCAGCGUGAGCAUGG 17 1977

HSV2-UL19-984 - GACCUACGCGCUCAUGG 17 1224

HSV2-UL19-1689 + CACGGGCUGGCGGAUGG 17 1929

HSV2-UL19-1958 + GAGGCGCUGUUUGAUGG 17 2198

HSV2-UL19-1893 + ACAGCCGCAGAACCUGG 17 2133

HSV2-UL19-890 - CGUGUAUCGGGACCUGG 17 1130

HSV2-UL19-793 - CGUGCCCUACCCCCUGG 17 1033

HSV2-UL19-976 - GGUCCCCCCGGCCCUGG 17 1216

HSV2-UL19-833 - GUUUUCUGAACGCCUGG 17 1073

HSV2-UL19-723 - CGUCCAGGCCGUCCUGG 17 963

HSV2-UL19-790 - CAGGCUGGUCUUCCUGG 17 1030

HSV2-UL19-894 - GGCCCUGGCCCAGCUGG 17 1134

HSV2-UL19-1687 + GCUGCACCACGGGCUGG 17 1927

HSV2-UL19-1726 + UGUGGUCCAGGUGCUGG 17 1966 HSV2-UL19-945 - GCAUCCCGCCAAUCUGG 17 1185

HSV2-UL19-785 - CGUGCGCGCGGAUCUGG 17 1025

HSV2-UL19-1939 + GCACGCGCGUUGUCUGG 17 2179

HSV2-UL19-1837 + CCGGCGCCAUGGCGUGG 17 2077

HSV2-UL19-1029 - GCGCAACGCGGUCGUGG 17 1269

HSV2-UL19-1662 + CCCCGCCCGUUUCGUGG 17 1902

HSV2-UL19-759 - GUCCUUCCUGAAGGUGG 17 999

HSV2-UL19-1944 + GCAUAUCCAGCAGGUGG 17 2184

HSV2-UL19-1791 + UCCGGCAGUCUCGGUGG 17 2031

HSV2-UL19-1674 + ACCCGAACCCGGGGUGG 17 1914

HSV2-UL19-1931 + GUAGGGCACGUUGGUGG 17 2171

HSV2-UL19-1769 + AAUAGUAGAUUUUGUGG 17 2009

HSV2-UL19-1742 + GUUGGCCACCAGAUUGG 17 1982

HSV2-UL19-1930 + GGGGUAGGGCACGUUGG 17 2170

HSV2-UL19-1647 + CGAGGUAAAAGUUUUGG 17 1887

HSV2-UL19-1604 + GGCCGUGGUCGUACAUG 17 1844

HSV2-UL19-1097 - ACGCAGCGCCCGCGAUG 17 1337

HSV2-UL19-798 - CGUUCGUCCUGCCCCUG 17 1038

HSV2-UL19-975 - UGGUCCCCCCGGCCCUG 17 1215

HSV2-UL19-3080 - ACGUCCAGGCCGUCCUG 17 7463

HSV2-UL19-1829 + ACACCGCCGGGUAGCUG 17 2069

HSV2-UL19-1034 - UGGCGGGAAACCGGCUG 17 1274

HSV2-UL19-1587 + ACUUGAAGCAGGGGCUG 17 1827

HSV2-UL19-1725 + GUGUGGUCCAGGUGCUG 17 1965

HSV2-UL19-1938 + CGCACGCGCGUUGUCUG 17 2178

HSV2-UL19-813 - AGGACCACCAGGUUCUG 17 1053

HSV2-UL19-1093 - CUCGUGGAAGACCCGUG 17 1333

HSV2-UL19-772 - UGAUGGGCAAGGCCGUG 17 1012

HSV2-UL19-905 - UGCUGCCGCCCCUCGUG 17 1145

HSV2-UL19-994 - UCGGGUUCACCGUCGUG 17 1234

HSV2-UL19-943 - GGAGAUCGCCCCCGGUG 17 1183

HSV2-UL19-1774 + CAGUCGGCCGGCCGGUG 17 2014

HSV2-UL19-1014 - AAACGUGGACCUGGGUG 17 1254

HSV2-UL19-1721 + CGCCAUUUUGGAUGGUG 17 1961

HSV2-UL19-1591 + CGGGCGAGGCCCCGUUG 17 1831

HSV2-UL19-1739 + CGACGACGCGCCCGUUG 17 1979

HSV2-UL19-1784 + GCAGCCGGCCGUCGUUG 17 2024

HSV2-UL19-1652 + UGUCCGUAACGGGGUUG 17 1892

HSV2-UL19-966 - CGUCCACGCGCUGUUUG 17 1206

HSV2-UL19-1768 + CGUAAUAGUAGAUUUUG 17 2008

HSV2-UL19-1646 + CCGAGG UAAAAG U U U U G 17 1886

HSV2-UL19-810 - CGGCAGCUGUUUUUUUG 17 1050

HSV2-UL19-3102 - GCCUGUCUCUGUAAAAU 17 7485

HSV2-UL19-1021 - AACCCCGUUACGGACAU 17 1261

HSV2-UL19-786 - GCGGAUCUGGUGGCCAU 17 1026

HSV2-UL19-1985 + UCGAGAUAUCGUUGCAU 17 2225 HSV2-UL19-1741 + CGUGUUGGCCACCAGAU 17 1981

HSV2-UL19-1096 - UACGCAGCGCCCGCGAU 17 1336

HSV2-UL19-1086 - CAUCGUGGAAACGGGAU 17 1326

HSV2-UL19-1566 + UCGCUGGCGCAGGUGAU 17 1806

HSV2-UL19-1592 + GGCCCCGUUGAGGUGAU 17 1832

HSV2-UL19-770 - GUCACGGCGCUGGUGAU 17 1010

HSV2-UL19-887 - AGUGCAUGGCCGUGUAU 17 1127

HSV2-UL19-1868 + CGCGGUCAUCUGGCACU 17 2108

HSV2-UL19-1623 + GGCGCGCCGCGGCACCU 17 1863

HSV2-UL19-1013 - CGCGGAAACGUGGACCU 17 1253

HSV2-UL19-881 - UACAUCGUGACCUACCU 17 1121

HSV2-UL19-1022 - CCCCAAAACU U U U ACCU 17 1262

HSV2-UL19-797 - ACGUUCGUCCUGCCCCU 17 1037

HSV2-UL19-974 - CUGGUCCCCCCGGCCCU 17 1214

HSV2-UL19-1952 + CACCGGCACGUCGGCCU 17 2192

HSV2-UL19-1782 + GUCGGCCGCGCGGGCCU 17 2022

HSV2-UL19-1816 + CACGGCCAUGCACUCCU 17 2056

HSV2-UL19-3081 - AACGUCCAGGCCGUCCU 17 7464

HSV2-UL19-739 - GACACGAGCUUCUUCCU 17 979

HSV2-UL19-1809 + AAAG UCGUCCACCAGCU 17 2049

HSV2-UL19-1802 + CAUCAGGUGAUUCAGCU 17 2042

HSV2-UL19-1977 + AAAGCUCCGCUUCAGCU 17 2217

HSV2-UL19-899 - CUGCCGGGCCCGGAGCU 17 1139

HSV2-UL19-865 - GCCCGGGGCCUGGAGCU 17 1105

HSV2-UL19-1666 + CGCCUCCGACGCGCGCU 17 1906

HSV2-UL19-1735 + CAGCGCCGUCGUGCGCU 17 1975

HSV2-UL19-1754 + UCGGUCACGGGAUCGCU 17 1994

HSV2-UL19-1033 - GUGGCGGGAAACCGGCU 17 1273

HSV2-UL19-1660 + CACGUUUCCGCGCGGCU 17 1900

HSV2-UL19-1630 + AAAGACCGGGAGGGGCU 17 1870

HSV2-UL19-1724 + GGUGUGGUCCAGGUGCU 17 1964

HSV2-UL19-1789 + CGUCAAUCCGGCAGUCU 17 2029

HSV2-UL19-1937 + GCGCACGCGCGUUGUCU 17 2177

HSV2-UL19-1667 + G G AAAACAG CACG U U CU 17 1907

HSV2-UL19-999 - UCGGAGGCGUACUUUCU 17 1239

HSV2-UL19-1554 + GAGAUACUGCGUAAAGU 17 1794

HSV2-UL19-1785 + GUCGUUGCGGUUAAAGU 17 2025

HSV2-UL19-1710 + CAGGGCCGGGGGGAAGU 17 1950

HSV2-UL19-1771 + GUGGUGGACGGUCCAGU 17 2011

HSV2-UL19-1690 + GAUGGAGGAGAAGUAGU 17 1930

HSV2-UL19-1951 + GUAGGUCACCGGCACGU 17 2191

HSV2-UL19-1929 + CAGGGGGUAGGGCACGU 17 2169

HSV2-UL19-775 - CGGAGCCUGGACGACGU 17 1015

HSV2-UL19-1813 + GGCCAGGGCCUCGACGU 17 2053

HSV2-UL19-1858 + GCCGGGAAGCUCGACGU 17 2098

HSV2-UL19-1069 - GGCCACGGCCAACCCGU 17 1309 HSV2-UL19-1949 + CAAG ACCAU CU CGCCG U 17 2189

HSV2-UL19-1872 + CCACCGGACCCGGCCGU 17 2112

HSV2-UL19-819 - AACAUCGACGCGGCCGU 17 1059

HSV2-UL19-1732 + GGUGUUGGCGCCCGCGU 17 1972

HSV2-UL19-1681 + CCCCGCCAUGAGCGCGU 17 1921

HSV2-UL19-996 - GUUUUCCGAGCGCGCGU 17 1236

HSV2-UL19-1556 + GUGGGUCUCUCGCGCGU 17 1796

HSV2-UL19-1712 + GGGGAAGUUGGGCGCGU 17 1952

HSV2-UL19-1778 + CCGGUCGUCGGCGGCGU 17 2018

HSV2-UL19-866 - GGCCUGGAGCUCGGCGU 17 1106

HSV2-UL19-1776 + CCGGUGCGGCCGGUCGU 17 2016

HSV2-UL19-1863 + CAGCUCCAGAGCCAGGU 17 2103

HSV2-UL19-1948 + CAGCGCCGUGACGAGGU 17 2188

HSV2-UL19-1821 + GAGGUCGCCCCCGAGGU 17 2061

HSV2-UL19-839 - CCACGGCCGGGUCCGGU 17 1079

HSV2-UL19-816 - UCCAUGGAGAACGCGGU 17 1056

HSV2-UL19-1596 + CUGCGACGCCCACGGGU 17 1836

HSV2-UL19-1747 + GGCGGGAUGCAGCGGGU 17 1987

HSV2-UL19-1568 + GGCGCAGGUGAUCGGGU 17 1808

HSV2-UL19-1751 + CAGCGGGUGGGCGGGGU 17 1991

HSV2-UL19-1927 + GGCGCCCACCAGGGGGU 17 2167

HSV2-UL19-794 - GUGCCCUACCCCCUGGU 17 1034

HSV2-UL19-1993 + CACGUGCAGCAUCUGGU 17 2233

HSV2-UL19-1714 + CGCGUUGGCCACGUGGU 17 1954

HSV2-UL19-1729 + GUCGAAGAUGCGCAUGU 17 1969

HSV2-UL19-1582 + AUGUUUGGCCGUGAUGU 17 1822

HSV2-UL19-726 - GAUGCUGCACGUGCUGU 17 966

HSV2-UL19-1740 + CAUGCGCUUGACCGUGU 17 1980

HSV2-UL19-1962 + CGGCCGCCCGCGCGUGU 17 2202

HSV2-UL19-906 - GCUGCCGCCCCUCGUGU 17 1146

HSV2-UL19-1731 + GGCCGUCGACGCGGUGU 17 1971

HSV2-UL19-1015 - AACGUGGACCUGGGUGU 17 1255

HSV2-UL19-1864 + AGCCAGGUUGGCGUUGU 17 2104

HSV2-UL19-1037 - CAGCCCCUCCCGGUCUU 17 1277

HSV2-UL19-1711 + AGGGCCGGGGGGAAGUU 17 1951

HSV2-UL19-992 - GGCCUCCACCCCGGGUU 17 1232

HSV2-UL19-1581 + CUCCAGGCAGCGAUGUU 17 1821

HSV2-UL19-1719 + AGAAGUAUUCGCCAUUU 17 1959

HSV2-UL19-1644 + GGCCGAGGUAAAAGUUU 17 1884

HSV2-UL19-1645 + GCCGAGGUAAAAGUUUU 17 1885

HSV2-UL19-808 - CCCGGCAGCUGUUUUUU 17 1048

HSV2-UL19-809 - CCGGCAGCUGUUUUUUU 17 1049

HSV2-UL19-587 - CUGGACCACACCAUCCAAAA 20 827

HSV2-UL19-624 - CCAGGUGGCCCGCCACGAAA 20 864

HSV2-UL19-706 - GGAGCGUCUCAUCGUGGAAA 20 946

HSV2-UL19-722 - UACGACGCCUCCCCGCUAAA 20 962 HSV2-UL19-569 - GUCAAGCGCAUGUUCCACAA 20 809

HSV2-UL19-351 - AUGCAACGAUAUCUCGACAA 20 591

HSV2-UL19-696 - UACGGGGACCUGCUGUACAA 20 936

HSV2-UL19-699 - AACGGGGCCUAUCACCUCAA 20 939

HSV2-UL19-479 - GCCACCUGGCGCGUGGUCAA 20 719

HSV2-UL19-1151 + GGGU UGGCCGUGGCCGCGAA 20 1391

HSV2-UL19-433 - GCAGCUGUUUUUUUGGGGAA 20 673

HSV2-UL19-386 - UACGGCGAGAUGGUCUUGAA 20 626

HSV2-UL19-721 - CUACGACGCCUCCCCGCUAA 20 961

HSV2-UL19-1201 + GAGGUUGCCCAUGUCCGUAA 20 1441

HSV2-UL19-575 - GCAGGUGCUGGCG CACAACA 20 815

HSV2-UL19-568 - CGCCAAUCUGGUGGCCAACA 20 808

HSV2-UL19-561 - GUACGCCACGCUGCAGAACA 20 801

HSV2-UL19-1170 + GGUGGCGAUGAACUCACACA 20 1410

HSV2-UL19-1442 + CAUGAGCGAAGGAUGACACA 20 1682

HSV2-UL19-642 - CCG CAACCCCG U U ACG G ACA 20 882

HSV2-UL19-680 - CGGCGUGUACGCGGGGGACA 20 920

HSV2-UL19-1367 + GGCCACCAGGUCCCGAUACA 20 1607

HSV2-UL19-1229 + CGUCUUGAGCUGGUGAUACA 20 1469

HSV2-UL19-1186 + GACCGCGUUGCGCAGGUACA 20 1426

HSV2-UL19-1476 + CAG G U CCAU GG CG CCCACCA 20 1716

HSV2-UL19-1236 + CUCGCGGGCGUGCUGCACCA 20 1476

HSV2-UL19-685 - AUAGCCCUCAUGUACGACCA 20 925

HSV2-UL19-1531 + CCGCUUCAGCUCGGCGACCA 20 1771

HSV2-UL19-355 - CAACGGGCGCCUGGCGACCA 20 595

HSV2-UL19-1251 + CCCCAGGGCCGGGGGGACCA 20 1491

HSV2-UL19-664 - CGGCGCGCCGGCAUGGACCA 20 904

HSV2-UL19-369 - CUGGCUCGUGGACCUGACCA 20 609

HSV2-UL19-1244 + GGAGAAGUAGUUGGCCCCCA 20 1484

HSV2-UL19-1470 + GGCCGGGUUGAACAGCCCCA 20 1710

HSV2-UL19-1146 + GAGAACCGCUGCGACGCCCA 20 1386

HSV2-UL19-456 - UGGCGGCAGCGCCUCGCCCA 20 696

HSV2-UL19-489 - CGGCGUUGGCCGCCACGCCA 20 729

HSV2-UL19-1395 + CGCCACCGGACACAGCGCCA 20 1635

HSV2-UL19-1376 + CUGAGUCACGAGCCGCGCCA 20 1616

HSV2-UL19-1387 + GGCUAUGGUAGCCGGCGCCA 20 1627

HSV2-UL19-417 - CUACCCCCUGGUGGGCGCCA 20 657

HSV2-UL19-1520 + CGUCAGGCGGGGCACGGCCA 20 1760

HSV2-UL19-422 - GGGGCUGUUCAACCCGGCCA 20 662

HSV2-UL19-639 - CUACACCGCCGUCGCGGCCA 20 879

HSV2-UL19-689 - GGCGCGGCCCUUCGCGGCCA 20 929

HSV2-UL19-1230 + GAGCUGGUGAUACAGGGCCA 20 1470

HSV2-UL19-1541 + CAU G G G CA ACAG CAG G G CCA 20 1781

HSV2-UL19-1363 + GUCGUCCACCAGCUGGGCCA 20 1603

HSV2-UL19-668 - CAUCGCCACCCCCGUGGCCA 20 908

HSV2-UL19-1165 + GUAGUUGAUGUCCGUGGCCA 20 1405 HSV2-UL19-710 - AACGGGAUCGGCGGUAUCCA 20 950

HSV2-UL19-1466 + GUGCGCGGCGAAGCGCUCCA 20 1706

HSV2-UL19-1485 + GGCGUAGAUGCGUCUCUCCA 20 1725

HSV2-UL19-1443 + GGUCCCGACCGCGUUCUCCA 20 1683

HSV2-UL19-1474 + CAGGACGAACGUCAGGUCCA 20 1714

HSV2-UL19-1345 + GCAGUCUCGGUGGCGGUCCA 20 1585

HSV2-UL19-436 - CCAGGUUCUGCGGCUGUCCA 20 676

HSV2-UL19-1137 + GCGGUGAAGAACUUGAAGCA 20 1377

HSV2-UL19-1539 + UCGU UGCAUGGGCAACAGCA 20 1779

HSV2-UL19-1288 + CAGCACCUGCAGCGUGAGCA 20 1528

HSV2-UL19-1112 + AUCGCGGGCGCUGCGUAGCA 20 1352

HSV2-UL19-1122 + GCUUCCUGAAACAGGCCGCA 20 1362

HSV2-UL19-1498 + CACGUCGUCCAGGCUCCGCA 20 1738

HSV2-UL19-1256 + GUCGCGCGCCAGGUCGCGCA 20 1496

HSV2-UL19-1359 + GCCCAGCUCCGGGCCCGGCA 20 1599

HSV2-UL19-663 - GGUGCCGCGGCGCGCCGGCA 20 903

HSV2-UL19-347 - GGGGGCGUUUGAGCGCGGCA 20 587

HSV2-UL19-1519 + GGCGUGCGUCAGGCGGGGCA 20 1759

HSV2-UL19-393 - CACGGCGCUGGUGAUGGGCA 20 633

HSV2-UL19-362 - CACGAGCUUCUUCCUGGGCA 20 602

HSV2-UL19-508 - CGACCUCCCCGAGGAGUGCA 20 748

HSV2-UL19-1536 + GUUGUCGAGAUAUCGUUGCA 20 1776

HSV2-UL19-1162 + GCGCGUCCCCGCGGGUUGCA 20 1402

HSV2-UL19-703 - CUUCACCGCGGCCGACAUCA 20 943

HSV2-UL19-605 - CGCGCUGACCUACGCGCUCA 20 845

HSV2-UL19-585 - GCACGCCGGCGUGCUGCUCA 20 825

HSV2-UL19-389 - GAACGGGGCCAACCUCGUCA 20 629

HSV2-UL19-1304 + CGGGUGGGCGGGGUCGGUCA 20 1544

HSV2-UL19-497 - CUGCUGCAAGCCGCGAUUCA 20 737

HSV2-UL19-610 - CCU G U AU CACCAG CU CAAGA 20 850

HSV2-UL19-1318 + GCAGCAGCGCCCCCGCGAGA 20 1558

HSV2-UL19-385 - GCCGGUGACCUACGGCGAGA 20 625

HSV2-UL19-349 - GCUGCACGUGCUGUUGGAGA 20 589

HSV2-UL19-1338 + GUUGCACGCCGCCGCGUAGA 20 1578

HSV2-UL19-543 - GCCGACUUUAACCGCAACGA 20 783

HSV2-UL19-1348 + GCCGUCGCAGUCCCACACGA 20 1588

HSV2-UL19-1221 + GAAGCGGUCCUGCCGCACGA 20 1461

HSV2-UL19-576 - CAACAUGGCCGAGCGCACGA 20 816

HSV2-UL19-1440 + GUCGUGGUUCACGCCCCCGA 20 1680

HSV2-UL19-1441 + GCGUCGAUGUUCAUGAGCGA 20 1681

HSV2-UL19-717 - CUGCUACGCAGCGCCCGCGA 20 957

HSV2-UL19-1207 + GACGGUGCCCGUGGCCGCGA 20 1447

HSV2-UL19-1150 + CGGGUUGGCCGUGGCCGCGA 20 1390

HSV2-UL19-1527 + CCUCGAUGGCCUCGCGGCGA 20 1767

HSV2-UL19-542 - GGCGGCGUGCAACGUGGCGA 20 782

HSV2-UL19-529 - CCCCUCGUGUGGGACUGCGA 20 769 HSV2-UL19-1525 + GUCCACGAGCCAGGCCUCGA 20 1765

HSV2-UL19-1436 + GUACGGAUUCGCGGCCUCGA 20 1676

HSV2-UL19-580 - GGGCGCCAACACCGCGUCGA 20 820

HSV2-UL19-571 - AACGGGCGCGUCGUCGUCGA 20 811

HSV2-UL19-375 - ACGCGCGGGCGGCCGGUCGA 20 615

HSV2-UL19-581 - GCCAACAUGCGCAUCUUCGA 20 821

HSV2-UL19-682 - GUGUACGCGGGGGACAAGGA 20 922

HSV2-UL19-1547 + GCGCUCAAACGCCCCCAGGA 20 1787

HSV2-UL19-1506 + GGUGUCCUCCACCU UCAGGA 20 1746

HSV2-UL19-1140 + CUUGAAGCAGGGGCUGAGGA 20 1380

HSV2-UL19-1521 + AGGCGGGGCACGGCCACGGA 20 1761

HSV2-UL19-1383 + GUCCUCGAACGCCCCGCGGA 20 1623

HSV2-UL19-1240 + CUGCACCACGGGCUGGCGGA 20 1480

HSV2-UL19-1205 + GUCCGUAACGGGGUUGCGGA 20 1445

HSV2-UL19-1179 + GGCGCAGCCAAAGACCGGGA 20 1419

HSV2-UL19-1450 + AAAAAAACAGCUGCCGGGGA 20 1690

HSV2-UL19-3073 + GUUGCGCGCGAGCUGCUGGA 20 7456

HSV2-UL19-1268 + GCCCGCAAACAGCGCGUGGA 20 1508

HSV2-UL19-1322 + AUAGUAGAUUUUGUGGUGGA 20 1562

HSV2-UL19-1272 + GAAGUAUUCGCCAUUUUGGA 20 1512

HSV2-UL19-1157 + CUGGCCGUGGUCGUACAUGA 20 1397

HSV2-UL19-379 - CCUCCUGCAGUCCUUCCUGA 20 619

HSV2-UL19-391 - CCUCGUCACGGCGCUGGUGA 20 631

HSV2-UL19-1509 + CUGCAGGAGGCGCUGUUUGA 20 1749

HSV2-UL19-1346 + CCAGGGCCGCGUGUCGCAUA 20 1586

HSV2-UL19-384 - GCCGACGUGCCGGUGACCUA 20 624

HSV2-UL19-1487 + GAAGACCAGCCUGUCGCCUA 20 1727

HSV2-UL19-1385 + CGCCCCGCGGACGGCGGCUA 20 1625

HSV2-UL19-1434 + GCCGCGACGUACGCCCCGUA 20 1674

HSV2-UL19-446 - GUCGAGGCCGCGAAUCCGUA 20 686

HSV2-UL19-693 - GCGUCGCAGCGGU UCUCGUA 20 933

HSV2-UL19-1480 + AUGGCGCCCACCAGGGGGUA 20 1720

HSV2-UL19-641 - CACCG UCCGCAACCCCGU UA 20 881

HSV2-UL19-625 - CAG G U G GCCCGCCACG AAAC 20 865

HSV2-UL19-653 - ACGCGGUCGUGGCGGGAAAC 20 893

HSV2-UL19-707 - GAGCGUCUCAUCGUGGAAAC 20 947

HSV2-UL19-1121 + UCGGGUAGGCUUCCUGAAAC 20 1361

HSV2-UL19-570 - UCAAGCGCAUGUUCCACAAC 20 810

HSV2-UL19-352 - UGCAACGAUAUCUCGACAAC 20 592

HSV2-UL19-697 - ACGGGGACCUGCUGUACAAC 20 937

HSV2-UL19-700 - ACGGGGCCUAUCACCUCAAC 20 940

HSV2-UL19-402 - AGGAGCAACUCGAGGCGAAC 20 642

HSV2-UL19-387 - ACGGCGAGAUGGUCUUGAAC 20 627

HSV2-UL19-1202 + AGGUUGCCCAUGUCCGUAAC 20 1442

HSV2-UL19-1237 + UCGCGGGCGUGCUGCACCAC 20 1477

HSV2-UL19-665 - GGCGCGCCGGCAUGGACCAC 20 905 HSV2-UL19-1421 + UCUGGCACUCGGCGACCCAC 20 1661

HSV2-UL19-1147 + AGAACCGCU GCGACGCCCAC 20 1387

HSV2-UL19-1393 + CGGGCGUCACGAAACGCCAC 20 1633

HSV2-UL19-640 - UACACCGCCGUCGCGGCCAC 20 880

HSV2-UL19-1231 + AGCUGGUGAUACAGGGCCAC 20 1471

HSV2-UL19-1166 + UAGUUGAUGUCCGUGGCCAC 20 1406

HSV2-UL19-1123 + CUUCCUGAAACAGGCCGCAC 20 1363

HSV2-UL19-548 - CCGCCGACGACCGGCCGCAC 20 788

HSV2-UL19-1308 + AUCGCUCGGGCACUCCUCAC 20 1548

HSV2-UL19-1502 + ACCAUCUCGCCGUAGGUCAC 20 1742

HSV2-UL19-1305 + GGGUGGGCGGGGUCGGUCAC 20 1545

HSV2-UL19-1176 + ACCUGGGCGCAGCCAAAGAC 20 1416

HSV2-UL19-611 - CU G U AU CACCAG CU CAAG AC 20 851

HSV2-UL19-3103 + GAAGCCAAUUUUACAGAGAC 20 7486

HSV2-UL19-1339 + UUGCACGCCGCCGCGUAGAC 20 1579

HSV2-UL19-547 - CGGCCGACGCCGCCGACGAC 20 787

HSV2-UL19-550 - GGCCGCACCGGCCGGCCGAC 20 790

HSV2-UL19-409 - AUCUGGUGGCCAUAGGCGAC 20 649

HSV2-UL19-1437 + UACGGAUUCGCGGCCUCGAC 20 1677

HSV2-UL19-1513 + GUGACCAGCACCCCGUCGAC 20 1753

HSV2-UL19-572 - ACGGGCGCGUCGUCGUCGAC 20 812

HSV2-UL19-376 - CGCGCGGGCGGCCGGUCGAC 20 616

HSV2-UL19-582 - CCAACAUGCGCAUCUUCGAC 20 822

HSV2-UL19-1141 + UUGAAGCAGGGGCUGAGGAC 20 1381

HSV2-UL19-1402 + GGCCUGGAUCGCCCCCGGAC 20 1642

HSV2-UL19-1269 + CCCGCAAACAGCGCGUGGAC 20 1509

HSV2-UL19-1228 + CCGUCUUGAGCUGGUGAUAC 20 1468

HSV2-UL19-500 - CUCAGUGCAUCACCAGCUAC 20 740

HSV2-UL19-447 - UCGAGGCCGCGAAUCCGUAC 20 687

HSV2-UL19-694 - CGUCGCAGCGGUUCUCGUAC 20 934

HSV2-UL19-463 - GCAGCCCGACAACGCCAACC 20 703

HSV2-UL19-421 - GCCCCUGGGGCUGUUCAACC 20 661

HSV2-UL19-1444 + CCAUGGACAGCCGCAGAACC 20 1684

HSV2-UL19-1222 + CACGACG G U G AACCCG AACC 20 1462

HSV2-UL19-403 - GGAGCAACUCGAGGCGAACC 20 643

HSV2-UL19-434 - UUUUUGGGGAAAGGACCACC 20 674

HSV2-UL19-1475 + UCAGGUCCAUGGCGCCCACC 20 1715

HSV2-UL19-477 - CGGGGGCGAUCCAGGCCACC 20 717

HSV2-UL19-428 - GGUGCCCGCCCCCGGCCACC 20 668

HSV2-UL19-1216 + CCGUUUCGUGGCGGGCCACC 20 1456

HSV2-UL19-1366 + GGGCCUCGACGUGGGCCACC 20 1606

HSV2-UL19-586 - GCUCAUGGCCCCCCAGCACC 20 826

HSV2-UL19-1174 + UGCCGGCGCGCCGCGGCACC 20 1414

HSV2-UL19-1463 + GGUGGCCGGGGGCGGGCACC 20 1703

HSV2-UL19-1309 + UCGCUCGGGCACUCCUCACC 20 1549

HSV2-UL19-1177 + CCUGGGCGCAGCCAAAGACC 20 1417 HSV2-UL19-686 - CGACCACGGCCAGAGCGACC 20 926

HSV2-UL19-526 - GAAUCACCUGAUGCGCGACC 20 766

HSV2-UL19-592 - CCCCCCGGCCCUGCGCGACC 20 832

HSV2-UL19-1530 + UCCGCUUCAGCUCGGCGACC 20 1770

HSV2-UL19-354 - ACAACGGGCG CCU GG CG ACC 20 594

HSV2-UL19-1422 + ACUCGGCGACCCACCGGACC 20 1662

HSV2-UL19-511 - CAUGGCCGUGUAUCGGGACC 20 751

HSV2-UL19-426 - CGCCGCGCACGCCGGGGACC 20 666

HSV2-UL19-1250 + CCCCCAGGGCCGGGGGGACC 20 1490

HSV2-UL19-634 - GCCGCGCGGAAACGUGGACC 20 874

HSV2-UL19-495 - GUUCGAGGACCGCAGCUACC 20 735

HSV2-UL19-1534 + CAGGGUCGCCCGGGCAACCC 20 1774

HSV2-UL19-1223 + ACGACGGUGAACCCGAACCC 20 1463

HSV2-UL19-545 - CCGGCUGCUGCACAACACCC 20 785

HSV2-UL19-1209 + CGGCGGUGUAGCCCACACCC 20 1449

HSV2-UL19-612 - CU CAAG ACG GG CCU CCACCC 20 852

HSV2-UL19-1125 + UCCACGAGCUCGCGGCACCC 20 1365

HSV2-UL19-358 - CAGGGUUGCCCGGGCGACCC 20 598

HSV2-UL19-613 - UCAAGACGGGCCUCCACCCC 20 853

HSV2-UL19-414 - CACCAACGUGCCCUACCCCC 20 654

HSV2-UL19-1546 + UGCCGCGCUCAAACGCCCCC 20 1786

HSV2-UL19-1243 + AGGAGAAGUAGUUGGCCCCC 20 1483

HSV2-UL19-470 - UCCCGGCGGCGAAGUCCCCC 20 710

HSV2-UL19-593 - CCUGGCGCGCGACGUCCCCC 20 833

HSV2-UL19-594 - CGACGUCCCCCUGGUCCCCC 20 834

HSV2-UL19-591 - CAACG CG CCCAACU U CCCCC 20 831

HSV2-UL19-1469 + UGGCCGGGUUGAACAGCCCC 20 1709

HSV2-UL19-427 - GGGGACCUGGUGCCCGCCCC 20 667

HSV2-UL19-564 - GUGGUCCCGGAGAUCGCCCC 20 804

HSV2-UL19-1401 + CAGGUGGCCUGGAUCGCCCC 20 1641

HSV2-UL19-1392 + AACGCCGAGCUCCAGGCCCC 20 1632

HSV2-UL19-480 - GGUCAACGGCAACCUGCCCC 20 720

HSV2-UL19-418 - CCUGACGUUCGUCCUGCCCC 20 658

HSV2-UL19-350 - GUUGGAGAAGGCGCCUCCCC 20 590

HSV2-UL19-484 - GGUGGCGU UUCGUGACGCCC 20 724

HSV2-UL19-660 - CCCGGUCUUUGGCUGCGCCC 20 900

HSV2-UL19-498 - CGAGCACGUGU UCUGCGCCC 20 738

HSV2-UL19-1533 + CUCGGCGACCAGGGUCGCCC 20 1773

HSV2-UL19-1391 + CAACGCCGAGCUCCAGGCCC 20 1631

HSV2-UL19-514 - GGUGGCCCACGUCGAGGCCC 20 754

HSV2-UL19-413 - GCUGGUCU UCCUGGAGGCCC 20 653

HSV2-UL19-595 - CCCCCUGGUCCCCCCGGCCC 20 835

HSV2-UL19-452 - GUCGCGGCCCCGGCCGGCCC 20 692

HSV2-UL19-531 - CCUUAUGCGACACGCGGCCC 20 771

HSV2-UL19-450 - CGGGGCGUACGUCGCGGCCC 20 690

HSV2-UL19-1528 + GGCGAUGGCCCGCCUUGCCC 20 1768 HSV2-UL19-357 - CCUGGCGACCAGGGUUGCCC 20 597

HSV2-UL19-429 - AGCCCCGCGCGUUCCCUCCC 20 669

HSV2-UL19-1464 + GGGGGCGGGCACCAGGUCCC 20 1704

HSV2-UL19-1414 + CGGGGUGCAGCUCCAGAGCC 20 1654

HSV2-UL19-1524 + CGUGGUCAGGUCCACGAGCC 20 1764

HSV2-UL19-395 - GGGCAAGGCCGUGCGGAGCC 20 635

HSV2-UL19-454 - AGCAGCGUUUUCUGAACGCC 20 694

HSV2-UL19-424 - AGCGCUUCGCCGCGCACGCC 20 664

HSV2-UL19-483 - CGGUGGCGUUUCGUGACGCC 20 723

HSV2-UL19-1380 + GCAGCAGGUAAAACACCGCC 20 1620

HSV2-UL19-712 - GUGCAGUUUAAGCGCCCGCC 20 952

HSV2-UL19-1409 + GCCGGGGGGACU UCGCCGCC 20 1649

HSV2-UL19-1426 + GUGGGCGAGGCGCUGCCGCC 20 1666

HSV2-UL19-1394 + ACGCCACCGGACACAGCGCC 20 1634

HSV2-UL19-1352 + CGAGGGGCGGCAGCAGCGCC 20 1592

HSV2-UL19-1398 + GUUGCCGUUGACCACGCGCC 20 1638

HSV2-UL19-1375 + ACUGAGUCACGAGCCGCGCC 20 1615

HSV2-UL19-1153 + UGGCCGCGAAGGGCCGCGCC 20 1393

HSV2-UL19-1428 + CGCUGCUGCAUGUCCGCGCC 20 1668

HSV2-UL19-1254 + CCAGGGGGACGUCGCGCGCC 20 1494

HSV2-UL19-353 - AUAUCUCGACAACGGGCGCC 20 593

HSV2-UL19-1532 + GCUCGGCGACCAGGGUCGCC 20 1772

HSV2-UL19-1535 + CCCGGGCAACCCUGGUCGCC 20 1775

HSV2-UL19-367 - GCCGCGAGGCCAUCGAGGCC 20 607

HSV2-UL19-458 - GCAGCGCCUCGCCCACGGCC 20 698

HSV2-UL19-1548 + CAAACGCCCCCAGGACGGCC 20 1788

HSV2-UL19-1404 + GGAUCGCCCCCGGACCGGCC 20 1644

HSV2-UL19-1432 + UGUCCGCGCCGGGGCCGGCC 20 1672

HSV2-UL19-1246 + AGUAGUUGGCCCCCAGGGCC 20 1486

HSV2-UL19-1540 + G CAU G G G CAACAG CAG G G CC 20 1780

HSV2-UL19-1258 + GCGCCAGGUCGCGCAGGGCC 20 1498

HSV2-UL19-519 - CGACUUUACCCUGCCGGGCC 20 759

HSV2-UL19-1357 + UGCCCGCCCAGCUCCGGGCC 20 1597

HSV2-UL19-1333 + CGGCGUCGGCCGCGCGGGCC 20 1573

HSV2-UL19-486 - GUU UCGUGACGCCCGGGGCC 20 726

HSV2-UL19-1362 + AGUCGUCCACCAGCUGGGCC 20 1602

HSV2-UL19-1468 + CGGCGAAGCGCUCCAUGGCC 20 1708

HSV2-UL19-1400 + UGACCACGCGCCAGGUGGCC 20 1640

HSV2-UL19-1458 + GCGCGGGGCUCCGGGUGGCC 20 1698

HSV2-UL19-518 - GUGGACGACUUUACCCUGCC 20 758

HSV2-UL19-1447 + UCCCCAAAAAAACAGCUGCC 20 1687

HSV2-UL19-704 - CACGGCCAAACAUCGCUGCC 20 944

HSV2-UL19-356 - GCCUGGCGACCAGGGUUGCC 20 596

HSV2-UL19-476 - GGCCGGUCCGGGGGCGAUCC 20 716

HSV2-UL19-658 - GGGGCCGGCCCAGCCCCUCC 20 898

HSV2-UL19-1486 + UGCGUCUCUCCAGGGCCUCC 20 1726 HSV2-UL19-1356 + GAGCCUGCCCGCCCAGCUCC 20 1596

HSV2-UL19-622 - GUACUUUCUGGGCCAGCUCC 20 862

HSV2-UL19-1390 + GGCGGCCAACGCCGAGCUCC 20 1630

HSV2-UL19-1132 + UUUCCACGAUGAGACGCUCC 20 1372

HSV2-UL19-1455 + GAGGGAACGCGCGGGGCUCC 20 1695

HSV2-UL19-1313 + CCUCACCGGGGGCGAUCUCC 20 1553

HSV2-UL19-1484 + CGGCGUAGAUGCGUCUCUCC 20 1724

HSV2-UL19-1493 + CGAGUUCAUCCAGCGUCUCC 20 1733

HSV2-UL19-3074 - GCAGCUCGCGCGCAACGUCC 20 7457

HSV2-UL19-3092 - GCGCAACGUCCAGGCCGUCC 20 7475

HSV2-UL19-1497 + GGUGGCGGCCCACGUCGUCC 20 1737

HSV2-UL19-473 - GAAGUCCCCCCGGCCGGUCC 20 713

HSV2-UL19-1344 + GGCAGUCUCGGUGGCGGUCC 20 1584

HSV2-UL19-563 - GCUGCAGAACAUGGUGGUCC 20 803

HSV2-UL19-1274 + CAUUUUGGAUGGUGUGGUCC 20 1514

HSV2-UL19-468 - GUGGCCGACGUCGAGCUUCC 20 708

HSV2-UL19-411 - AGGCGACAGGCUGGUCUUCC 20 651

HSV2-UL19-360 - UUGCGACACGAGCUUCUUCC 20 600

HSV2-UL19-1136 + CGCGGUGAAGAACUUGAAGC 20 1376

HSV2-UL19-1538 + AUCGU UGCAUGGGCAACAGC 20 1778

HSV2-UL19-1145 + GAUAGGCCCCGUUGUACAGC 20 1385

HSV2-UL19-1360 + G G G U AAAG U CG U CCACCAG C 20 1600

HSV2-UL19-1184 + GGGGCUGGGCCGGCCCCAGC 20 1424

HSV2-UL19-515 - CGUCGAGGCCCUGGCCCAGC 20 755

HSV2-UL19-1494 + GCUCCUCCUGCAUAUCCAGC 20 1734

HSV2-UL19-1335 + CCUGGGUGUUGUGCAGCAGC 20 1575

HSV2-UL19-1189 + CGGCCGCGUUGUCUAGCAGC 20 1429

HSV2-UL19-35 + GCUGCUGGAUGGCGCGCAGC 20 463

HSV2-UL19-1297 + CCAGAUUGGCGGGAUGCAGC 20 1537

HSV2-UL19-1378 + CGUGAAUCGCGGCUUGCAGC 20 1618

HSV2-UL19-520 - UACCCUGCCGGGCCCGGAGC 20 760

HSV2-UL19-1217 + CGUGGCGGGCCACCUGGAGC 20 1457

HSV2-UL19-1227 + GGUGGAGGCCCGUCUUGAGC 20 1467

HSV2-UL19-1374 + UCGCGUGUUGUUCCAGUAGC 20 1614

HSV2-UL19-1111 + CAUCGCGGGCGCUGCGUAGC 20 1351

HSV2-UL19-1386 + CGGACGGCGGCUAUGGUAGC 20 1626

HSV2-UL19-1102 + ACAGAGACAGGCCCUUUAGC 20 1342

HSV2-UL19-1412 + CCCGCGAAGAAGUCGAACGC 20 1652

HSV2-UL19-1280 + GGGGGCCAUGAGCAGCACGC 20 1520

HSV2-UL19-423 - GAGCGCUUCGCCGCGCACGC 20 663

HSV2-UL19-584 - UUCGACGGGGCGCUGCACGC 20 824

HSV2-UL19-404 - CGAGGCGAACCGGGAGACGC 20 644

HSV2-UL19-579 - UGCUCCGCGGCGCCCGACGC 20 819

HSV2-UL19-534 - CGAGACUGCCGGAUUGACGC 20 774

HSV2-UL19-677 - GCGGCCGGCGGCGUGUACGC 20 917

HSV2-UL19-1379 + U G CAG CAG G U AAAACACCG C 20 1619 HSV2-UL19-558 - CGGGGGCGCUGCUGCACCGC 20 798

HSV2-UL19-671 - UCGCCGGCCCUGCAACCCGC 20 911

HSV2-UL19-1160 + CGCCGGCCGCGCGUCCCCGC 20 1400

HSV2-UL19-711 - CGUGCAGUUUAAGCGCCCGC 20 951

HSV2-UL19-1158 + GUCCCCCGCGUACACGCCGC 20 1398

HSV2-UL19-1408 + GGCCGGGGGGACUUCGCCGC 20 1648

HSV2-UL19-646 - AAAACUUUUACCUCGGCCGC 20 886

HSV2-UL19-492 - CUACCAUAGCCGCCGUCCGC 20 732

HSV2-UL19-1351 + ACGAGGGGCGGCAGCAGCGC 20 1591

HSV2-UL19-1452 + CUGCCGGGGAGGGAACGCGC 20 1692

HSV2-UL19-372 - UGACGCACGCCGACACGCGC 20 612

HSV2-UL19-1332 + GUCGGCGGCGUCGGCCGCGC 20 1572

HSV2-UL19-1152 + GUGGCCGCGAAGGGCCGCGC 20 1392

HSV2-UL19-1427 + ACGCUGCUGCAUGUCCGCGC 20 1667

HSV2-UL19-662 - GCCCAGGUGCCGCGGCGCGC 20 902

HSV2-UL19-1255 + CGUCGCGCGCCAGGUCGCGC 20 1495

HSV2-UL19-390 - GGCCAACCUCGUCACGGCGC 20 630

HSV2-UL19-490 - UGGCCGCCACGCCAUGGCGC 20 730

HSV2-UL19-1117 + CAGGGCGGGGUCGCUGGCGC 20 1357

HSV2-UL19-1185 + CCGCCACGACCGCGUUGCGC 20 1425

HSV2-UL19-1110 + CGCGUGGGCCUCCCCAUCGC 20 1350

HSV2-UL19-502 - GUUCGUGAACGACUACUCGC 20 742

HSV2-UL19-1235 + CUCCCCCGCCGCGCUCUCGC 20 1475

HSV2-UL19-554 - GCUGGUGCCGGCCUUCUCGC 20 794

HSV2-UL19-1128 + GCGCUUAAACUGCACGUCGC 20 1368

HSV2-UL19-1116 + GCGUAGCAGGGCGGGGUCGC 20 1356

HSV2-UL19-466 - CCCGCGUUCGACUUCUUCGC 20 706

HSV2-UL19-669 - CGGACAUCAACUACUUUCGC 20 909

HSV2-UL19-364 - AGCUUCUUCCUGGGCAAGGC 20 604

HSV2-UL19-1319 + CAGCGCCCCCGCGAGAAGGC 20 1559

HSV2-UL19-410 - GGUGGCCAUAGGCGACAGGC 20 650

HSV2-UL19-1517 + CGUGUCGGCGUGCGUCAGGC 20 1757

HSV2-UL19-457 - GGCAGCGCCUCGCCCACGGC 20 697

HSV2-UL19-544 - ACUUUAACCGCAACGACGGC 20 784

HSV2-UL19-654 - GGUCGUGGCGGGAAACCGGC 20 894

HSV2-UL19-549 - CGACGACCGGCCGCACCGGC 20 789

HSV2-UL19-1403 + UGGAUCGCCCCCGGACCGGC 20 1643

HSV2-UL19-1127 + CGAGCUCGCGGCACCCCGGC 20 1367

HSV2-UL19-471 - GGCGGCGAAGUCCCCCCGGC 20 711

HSV2-UL19-451 - GCGUACGUCGCGGCCCCGGC 20 691

HSV2-UL19-1358 + CGCCCAGCUCCGGGCCCGGC 20 1598

HSV2-UL19-1325 + GGACGGUCCAGUCGGCCGGC 20 1565

HSV2-UL19-1431 + AUGUCCGCGCCGGGGCCGGC 20 1671

HSV2-UL19-602 - CACGCCCGCGAGAGCGCGGC 20 842

HSV2-UL19-674 - AACCCGCGGGGACGCGCGGC 20 914

HSV2-UL19-1211 + GGUCCACGUUUCCGCGCGGC 20 1451 HSV2-UL19-374 - CGCCGACACGCGCGGGCGGC 20 614

HSV2-UL19-617 - CGGGUUCACCGUCGUGCGGC 20 857

HSV2-UL19-1327 + AGUCGGCCGGCCGGUGCGGC 20 1567

HSV2-UL19-1324 + UGGUGGACGGUCCAGUCGGC 20 1564

HSV2-UL19-1245 + AAGUAGUUGGCCCCCAGGGC 20 1485

HSV2-UL19-1114 + CGGGCGCUGCGUAGCAGGGC 20 1354

HSV2-UL19-1257 + CGCGCCAGGUCGCGCAGGGC 20 1497

HSV2-UL19-1163 + GUCCCCGCGGGUUGCAGGGC 20 1403

HSV2-UL19-627 - UGGCCCGCCACGAAACGGGC 20 867

HSV2-UL19-1238 + GGGCGUGCUGCACCACGGGC 20 1478

HSV2-UL19-666 - CGCCGGCAUGGACCACGGGC 20 906

HSV2-UL19-1270 + CAAACAGCGCGUGGACGGGC 20 1510

HSV2-UL19-524 - GGGCCCGGAGCUGGGCGGGC 20 764

HSV2-UL19-1472 + GGUUGAACAGCCCCAGGGGC 20 1712

HSV2-UL19-1397 + CCGGACACAGCGCCAGGGGC 20 1637

HSV2-UL19-1181 + AGCCAAAGACCGGGAGGGGC 20 1421

HSV2-UL19-1430 + CUGCAUGUCCGCGCCGGGGC 20 1670

HSV2-UL19-1462 + GGCUCCGGGUGGCCGGGGGC 20 1702

HSV2-UL19-657 - GGCGGGAAACCGGCUGGGGC 20 897

HSV2-UL19-523 - UGCCGGGCCCGGAGCUGGGC 20 763

HSV2-UL19-1183 + AAGACCGGGAGGGGCUGGGC 20 1423

HSV2-UL19-1301 + GCGGGAUGCAGCGGGUGGGC 20 1541

HSV2-UL19-1467 + GCGGCGAAGCGCUCCAUGGC 20 1707

HSV2-UL19-1290 + ACCUGCAGCGUGAGCAUGGC 20 1530

HSV2-UL19-607 - CUGACCUACGCGCUCAUGGC 20 847

HSV2-UL19-652 - CUGCGCAACGCGGUCGUGGC 20 892

HSV2-UL19-1215 + CCCCCCGCCCGUUUCGUGGC 20 1455

HSV2-UL19-1457 + CGCGCGGGGCUCCGGGUGGC 20 1697

HSV2-UL19-1295 + GUGU UGGCCACCAGAUUGGC 20 1535

HSV2-UL19-1172 + AUCCUGCCCGUGGUCCAUGC 20 1412

HSV2-UL19-399 - CCGCCACCUGCUGGAUAUGC 20 639

HSV2-UL19-532 - UGGACCGCCACCGAGACUGC 20 772

HSV2-UL19-1507 + CCACCUUCAGGAAGGACUGC 20 1747

HSV2-UL19-398 - CGACGUGGGCCGCCACCUGC 20 638

HSV2-UL19-517 - GGUGGACGACUUUACCCUGC 20 757

HSV2-UL19-1446 + U U CCCCAAAAAAACAG CU GC 20 1686

HSV2-UL19-3076 + GGACGU UGCGCGCGAGCUGC 20 7459

HSV2-UL19-573 - GCCCGCCAUGCUCACGCUGC 20 813

HSV2-UL19-383 - GGACACCGAGGCCGACGUGC 20 623

HSV2-UL19-551 - CAAAAUCUACUAUUACGUGC 20 791

HSV2-UL19-1275 + GGAUGGUGUGGUCCAGGUGC 20 1515

HSV2-UL19-574 - CAUGCUCACGCUGCAGGUGC 20 814

HSV2-UL19-378 - GCGGCCGGUCGACGGGGUGC 20 618

HSV2-UL19-552 - CUACUAUUACGUGCUGGUGC 20 792

HSV2-UL19-1161 + CGCGCGUCCCCGCGGGUUGC 20 1401

HSV2-UL19-589 - CCCGUCCACGCGCUGUUUGC 20 829 HSV2-UL19-566 - CCCGCUGCAUCCCGCCAAUC 20 806

HSV2-UL19-1340 + CGUGCCCCCCCGCGUCAAUC 20 1580

HSV2-UL19-1544 + CCAACAGCACGUGCAGCAUC 20 1784

HSV2-UL19-1353 + GCAGCGCCGGGUCGCGCAUC 20 1593

HSV2-UL19-1419 + UGAACUGCUCCGCGGUCAUC 20 1659

HSV2-UL19-406 - AACGCGCGUGCGCGCGGAUC 20 646

HSV2-UL19-1119 + GGUCGCUGGCGCAGGUGAUC 20 1359

HSV2-UL19-510 - GGAGUGCAUGGCCGUGUAUC 20 750

HSV2-UL19-504 - CGUACAUCGUGACCUACCUC 20 744

HSV2-UL19-1369 + UACACGGCCAUGCACUCCUC 20 1609

HSV2-UL19-1355 + UGAGCCUGCCCGCCCAGCUC 20 1595

HSV2-UL19-1307 + GGUCGGUCACGGGAUCGCUC 20 1547

HSV2-UL19-1154 + AGGGCCGCGCCGGGUCGCUC 20 1394

HSV2-UL19-525 - GGAGCUGGGCGGGCAGGCUC 20 765

HSV2-UL19-1454 + GGAGGGAACGCGCGGGGCUC 20 1694

HSV2-UL19-464 - CGACAACGCCAACCUGGCUC 20 704

HSV2-UL19-1312 + UCCUCACCGGGGGCGAUCUC 20 1552

HSV2-UL19-1473 + CCAGGGGCAGGACGAACGUC 20 1713

HSV2-UL19-442 - UGAACAUCGACGCGGCCGUC 20 682

HSV2-UL19-1285 + GCGGUGUUGGCGCCCGCGUC 20 1525

HSV2-UL19-1515 + CGCGCGUGUCGGCGUGCGUC 20 1755

HSV2-UL19-472 - CGAAGUCCCCCCGGCCGGUC 20 712

HSV2-UL19-439 - UGUCCAUGGAGAACGCGGUC 20 679

HSV2-UL19-459 - GCCUCGCCCACGGCCGGGUC 20 699

HSV2-UL19-1523 + ACGGCUGCGUCGCCGUGGUC 20 1763

HSV2-UL19-481 - CCUGCCCCUGGCGCUGUGUC 20 721

HSV2-UL19-1488 + CCGCGCGCACGCGCGUUGUC 20 1728

HSV2-UL19-1417 + AGAGCCAGGUUGGCGUUGUC 20 1657

HSV2-UL19-1505 + CCUCGGUGUCCUCCACCUUC 20 1745

HSV2-UL19-615 - CGGGCCUCCACCCCGGGUUC 20 855

HSV2-UL19-620 - CGCGUCGGAGGCGUACUUUC 20 860

HSV2-UL19-716 - AGACCCGUGCGGCCUGUUUC 20 956

HSV2-UL19-1105 + AGAUGAGAUACUGCGUAAAG 20 1345

HSV2-UL19-1220 + G CACG U U CU CGG U CACGAAG 20 1460

HSV2-UL19-1316 + UGGCGUACACGCGGUCGAAG 20 1556

HSV2-UL19-359 - CCCUGGUCGCCGAGCUGAAG 20 599

HSV2-UL19-1477 + AGGUCCAUGGCGCCCACCAG 20 1717

HSV2-UL19-1252 + CCCAGGGCCGGGGGGACCAG 20 1492

HSV2-UL19-1471 + GCCGGGUUGAACAGCCCCAG 20 1711

HSV2-UL19-1396 + GCCACCGGACACAGCGCCAG 20 1636

HSV2-UL19-1542 + AU G G G CAACAG CAG G G CCAG 20 1782

HSV2-UL19-1138 + CGGUGAAGAACUUGAAGCAG 20 1378

HSV2-UL19-1188 + GCGGCCGCGUUGUCUAGCAG 20 1428

HSV2-UL19-692 - CCAACCCGUGGGCGUCGCAG 20 932

HSV2-UL19-1129 + AAACUGCACGUCGCUGGCAG 20 1369

HSV2-UL19-1296 + ACCAGAUUGGCGGGAUGCAG 20 1536 HSV2-UL19-1349 + CCGUCGCAGUCCCACACGAG 20 1589

HSV2-UL19-683 - UGUACGCGGGGGACAAGGAG 20 923

HSV2-UL19-1180 + GCGCAGCCAAAGACCGGGAG 20 1420

HSV2-UL19-1101 + UACAGAGACAGGCCCUUUAG 20 1341

HSV2-UL19-633 - CACCCAGCCGCGCGGAAACG 20 873

HSV2-UL19-648 - CCCCCCGCUGCUAGACAACG 20 888

HSV2-UL19-698 - CGGGGACCUGCUGUACAACG 20 938

HSV2-UL19-649 - CGCCGUGUACCUGCGCAACG 20 889

HSV2-UL19-541 - CUACGCGGCGGCGUGCAACG 20 781

HSV2-UL19-701 - CGGGGCCUAUCACCUCAACG 20 941

HSV2-UL19-437 - GCGGCUGUCCAUGGAGAACG 20 677

HSV2-UL19-1411 + GCCCGCGAAGAAGUCGAACG 20 1651

HSV2-UL19-388 - CGGCGAGAUGGUCUUGAACG 20 628

HSV2-UL19-1203 + GGUUGCCCAUGUCCGUAACG 20 1443

HSV2-UL19-1347 + GGCCGUCGCAGUCCCACACG 20 1587

HSV2-UL19-530 - CGACGGCCUUAUGCGACACG 20 770

HSV2-UL19-1315 + UCUGCAGCGUGGCGUACACG 20 1555

HSV2-UL19-590 - GUU UGCGGGCGCCGACCACG 20 830

HSV2-UL19-1232 + GCUGGUGAUACAGGGCCACG 20 1472

HSV2-UL19-1167 + AGUUGAUGUCCGUGGCCACG 20 1407

HSV2-UL19-1265 + AGU UGGGCGCGUUGGCCACG 20 1505

HSV2-UL19-396 - CGUGCGGAGCCUGGACGACG 20 636

HSV2-UL19-578 - GUGCUCCGCGGCGCCCGACG 20 818

HSV2-UL19-440 - UUCGCUCAUGAACAUCGACG 20 680

HSV2-UL19-1438 + ACGGAUUCGCGGCCUCGACG 20 1678

HSV2-UL19-1364 + GCUGGGCCAGGGCCUCGACG 20 1604

HSV2-UL19-1282 + GCGCAUGUUGGCCGUCGACG 20 1522

HSV2-UL19-377 - GCGCGGGCGGCCGGUCGACG 20 617

HSV2-UL19-583 - CAACAUGCGCAUCUUCGACG 20 823

HSV2-UL19-1499 + CCAU CACCAG CGCCG UGACG 20 1739

HSV2-UL19-533 - CCGAGACUGCCGGAUUGACG 20 773

HSV2-UL19-539 - GGGGCACGAGCCCGUCUACG 20 779

HSV2-UL19-448 - CGAGGCCGCGAAUCCGUACG 20 688

HSV2-UL19-695 - GUCGCAGCGGUUCUCGUACG 20 935

HSV2-UL19-676 - CGCGGCCGGCGGCGUGUACG 20 916

HSV2-UL19-382 - CCUGAAGGUGGAGGACACCG 20 622

HSV2-UL19-557 - GCGGGGGCGCUGCUGCACCG 20 797

HSV2-UL19-1310 + CGCUCGGGCACUCCUCACCG 20 1550

HSV2-UL19-702 - CUGCUUCAAGUUCUUCACCG 20 942

HSV2-UL19-462 - CGCCGAGUGCCAGAUGACCG 20 702

HSV2-UL19-690 - UCGCGGCCACGGCCAACCCG 20 930

HSV2-UL19-670 - UUCGCCGGCCCUGCAACCCG 20 910

HSV2-UL19-1224 + CGACGGUGAACCCGAACCCG 20 1464

HSV2-UL19-667 - UGAGUUCAUCGCCACCCCCG 20 907

HSV2-UL19-1317 + GCGGUCGAAGCGGACCCCCG 20 1557

HSV2-UL19-1372 + CCUCGGGGAGGUCGCCCCCG 20 1612 HSV2-UL19-609 - GUACUUCAAGAUGAGCCCCG 20 849

HSV2-UL19-1382 + UGCGGUCCUCGAACGCCCCG 20 1622

HSV2-UL19-1194 + CUAGCAGCGGGGGGGCCCCG 20 1434

HSV2-UL19-507 - CCUCGGGGGCGACCUCCCCG 20 747

HSV2-UL19-1159 + CCGCCGGCCGCGCGUCCCCG 20 1399

HSV2-UL19-599 - CU CCU CCAU CCG CCAGCCCG 20 839

HSV2-UL19-485 - GUGGCGUUUCGUGACGCCCG 20 725

HSV2-UL19-1171 + CACACACGGCAUCCUGCCCG 20 1411

HSV2-UL19-1206 + GGGGU UGCGGACGGUGCCCG 20 1446

HSV2-UL19-425 - GCGCUUCGCCGCGCACGCCG 20 665

HSV2-UL19-713 - UGCAGUUUAAGCGCCCGCCG 20 953

HSV2-UL19-1429 + GCUGCUGCAUGUCCGCGCCG 20 1669

HSV2-UL19-1173 + UGGUCCAUGCCGGCGCGCCG 20 1413

HSV2-UL19-1286 + GGCGCCCGCGUCGGGCGCCG 20 1526

HSV2-UL19-1522 + CACGGACGGCUGCGUCGCCG 20 1762

HSV2-UL19-1405 + GAUCGCCCCCGGACCGGCCG 20 1645

HSV2-UL19-1423 + CGACCCACCGGACCCGGCCG 20 1663

HSV2-UL19-1433 + GUCCGCGCCGGGGCCGGCCG 20 1673

HSV2-UL19-1195 + GCGGGGGGGCCCCGCGGCCG 20 1435

HSV2-UL19-645 - CAAAACUUUUACCUCGGCCG 20 885

HSV2-UL19-1135 + UU UGGCCGUGAUGUCGGCCG 20 1375

HSV2-UL19-1247 + GUAGUUGGCCCCCAGGGCCG 20 1487

HSV2-UL19-1259 + CGCCAGGUCGCGCAGGGCCG 20 1499

HSV2-UL19-1155 + GCGCCGGGUCGCUCUGGCCG 20 1395

HSV2-UL19-1131 + GUCGCUGGCAGCGGUGGCCG 20 1371

HSV2-UL19-1459 + CGCGGGGCUCCGGGUGGCCG 20 1699

HSV2-UL19-1149 + CGACGCCCACGGGUUGGCCG 20 1389

HSV2-UL19-1448 + CCCCAAAAAAACAG C U G CCG 20 1688

HSV2-UL19-661 - UUGGCUGCGCCCAGGUGCCG 20 901

HSV2-UL19-1418 + GGGCUGCAUGAACUGCUCCG 20 1658

HSV2-UL19-577 - GACGGCGCUGCUGUGCUCCG 20 817

HSV2-UL19-370 - CACGGCGACGCAGCCGUCCG 20 610

HSV2-UL19-491 - GCUACCAUAGCCGCCGUCCG 20 731

HSV2-UL19-474 - AAGUCCCCCCGGCCGGUCCG 20 714

HSV2-UL19-1164 + GCGAAAGUAGUUGAUGUCCG 20 1404

HSV2-UL19-1190 + GGCCGCGUUGUCUAGCAGCG 20 1430

HSV2-UL19-1314 + GACCACCAUGUUCUGCAGCG 20 1554

HSV2-UL19-600 - GCAGCACGCCCGCGAGAGCG 20 840

HSV2-UL19-346 - GUCCUGGGGGCGUUUGAGCG 20 586

HSV2-UL19-1103 + CAGAGACAGGCCCUUUAGCG 20 1343

HSV2-UL19-1413 + CCGCGAAGAAGUCGAACGCG 20 1653

HSV2-UL19-1451 + GCUGCCGGGGAGGGAACGCG 20 1691

HSV2-UL19-371 - CUGACGCACGCCGACACGCG 20 611

HSV2-UL19-673 - CUGCAACCCGCGGGGACGCG 20 913

HSV2-UL19-535 - GAGACUGCCGGAUUGACGCG 20 775

HSV2-UL19-678 - CGGCCGGCGGCGUGUACGCG 20 918 HSV2-UL19-559 - GGGGGCGCUGCUGCACCGCG 20 799

HSV2-UL19-672 - CGCCGGCCCUGCAACCCGCG 20 912

HSV2-UL19-546 - GCACAACACCCAGGCCCGCG 20 786

HSV2-UL19-632 - UUCACGCUCACCCAGCCGCG 20 872

HSV2-UL19-365 - CAAGGCGGGCCAUCGCCGCG 20 605

HSV2-UL19-647 - AAACUUUUACCUCGGCCGCG 20 887

HSV2-UL19-1331 + CGUCGGCGGCGUCGGCCGCG 20 1571

HSV2-UL19-493 - UACCAUAGCCGCCGUCCGCG 20 733

HSV2-UL19-1210 + CCCAGGUCCACGUUUCCGCG 20 1450

HSV2-UL19-1267 + CGGCGCCCGCAAACAGCGCG 20 1507

HSV2-UL19-1453 + UGCCGGGGAGGGAACGCGCG 20 1693

HSV2-UL19-1107 + UAAAGUGGGUCUCUCGCGCG 20 1347

HSV2-UL19-478 - GAUCCAGGCCACCUGGCGCG 20 718

HSV2-UL19-405 - CCAGACAACGCGCGUGCGCG 20 645

HSV2-UL19-555 - CUGGUGCCGGCCUUCUCGCG 20 795

HSV2-UL19-1518 + GUGUCGGCGUGCGUCAGGCG 20 1758

HSV2-UL19-687 - ACGGCCAGAGCGACCCGGCG 20 927

HSV2-UL19-453 - GGCCCCGGCCGGCCCCGGCG 20 693

HSV2-UL19-603 - ACGCCCGCGAGAGCGCGGCG 20 843

HSV2-UL19-1115 + GGGCGCUGCGUAGCAGGGCG 20 1355

HSV2-UL19-628 - GGCCCGCCACGAAACGGGCG 20 868

HSV2-UL19-1142 + GCAGGGGCUGAGGACGGGCG 20 1382

HSV2-UL19-467 - GUUCGACUUCUUCGCGGGCG 20 707

HSV2-UL19-1425 + ACCGGACCCGGCCGUGGGCG 20 1665

HSV2-UL19-1302 + CGGGAUGCAGCGGGUGGGCG 20 1542

HSV2-UL19-1388 + UGGUAGCCGGCGCCAUGGCG 20 1628

HSV2-UL19-608 - UGACCUACGCGCUCAUGGCG 20 848

HSV2-UL19-499 - ACGUGU UCUGCGCCCUGGCG 20 739

HSV2-UL19-501 - CUGGAACAACACGCGAUGCG 20 741

HSV2-UL19-1465 + CACCAGGUCCCCGGCGUGCG 20 1705

HSV2-UL19-1377 + GUGCUCGUUGCCGUGAAUCG 20 1617

HSV2-UL19-1109 + GCGCGUGGGCCUCCCCAUCG 20 1349

HSV2-UL19-366 - CCAUCGCCGCGAGGCCAUCG 20 606

HSV2-UL19-705 - CUGCCUGGAGCGUCUCAUCG 20 945

HSV2-UL19-401 - UAUGCAGGAGGAGCAACUCG 20 641

HSV2-UL19-505 - GUACAUCGUGACCUACCUCG 20 745

HSV2-UL19-1526 + GCCAGGCCUCGAUGGCCUCG 20 1766

HSV2-UL19-1370 + ACACGGCCAUGCACUCCUCG 20 1610

HSV2-UL19-1124 + ACGGGUCUUCCACGAGCUCG 20 1364

HSV2-UL19-714 - GCCGGGGUGCCGCGAGCUCG 20 954

HSV2-UL19-368 - GGCCAUCGAGGCCUGGCUCG 20 608

HSV2-UL19-1234 + UCUCCCCCGCCGCGCUCUCG 20 1474

HSV2-UL19-553 - UGCUGGUGCCGGCCUUCUCG 20 793

HSV2-UL19-513 - GGACCUGGUGGCCCACGUCG 20 753

HSV2-UL19-449 - UCCGUACGGGGCGUACGUCG 20 689

HSV2-UL19-445 - CGUGAACCACGACCCCGUCG 20 685 HSV2-UL19-638 - UGUGGGCUACACCGCCGUCG 20 878

HSV2-UL19-443 - GAACAUCGACGCGGCCGUCG 20 683

HSV2-UL19-650 - GUACCUGCGCAACGCGGUCG 20 890

HSV2-UL19-1439 + UCGCGGCCUCGACGGGGUCG 20 1679

HSV2-UL19-1435 + GUACGCCCCGUACGGAUUCG 20 1675

HSV2-UL19-688 - CGACCCGGCGCGGCCCUUCG 20 928

HSV2-UL19-465 - CCCCGCGUUCGACUUCUUCG 20 705

HSV2-UL19-494 - CGCCGUCCGCGGGGCGUUCG 20 734

HSV2-UL19-1213 + UGACCCCCCCGCCCGUUUCG 20 1453

HSV2-UL19-681 - CGUGUACGCGGGGGACAAGG 20 921

HSV2-UL19-363 - GAGCUUCUUCCUGGGCAAGG 20 603

HSV2-UL19-380 - CCUGCAGUCCUUCCUGAAGG 20 620

HSV2-UL19-1478 + GGUCCAUGGCGCCCACCAGG 20 1718

HSV2-UL19-1253 + CCAGGGCCGGGGGGACCAGG 20 1493

HSV2-UL19-1399 + GCCGUUGACCACGCGCCAGG 20 1639

HSV2-UL19-623 - CUUUCUGGGCCAGCUCCAGG 20 863

HSV2-UL19-1495 + CCUCCUGCAUAUCCAGCAGG 20 1735

HSV2-UL19-400 - CCACCUGCUGGAUAUGCAGG 20 640

HSV2-UL19-1508 + CCUUCAGGAAGGACUGCAGG 20 1748

HSV2-UL19-1516 + GCGUGUCGGCGUGCGUCAGG 20 1756

HSV2-UL19-684 - GUACGCGGGGGACAAGGAGG 20 924

HSV2-UL19-1187 + CGCGUUGCGCAGGUACACGG 20 1427

HSV2-UL19-1168 + GUUGAUGUCCGUGGCCACGG 20 1408

HSV2-UL19-1208 + GGUGCCCGUGGCCGCGACGG 20 1448

HSV2-UL19-1384 + CUCGAACGCCCCGCGGACGG 20 1624

HSV2-UL19-1311 + GCUCGGGCACUCCUCACCGG 20 1551

HSV2-UL19-496 - CGAGGACCGCAGCUACCCGG 20 736

HSV2-UL19-1126 + ACGAGCUCGCGGCACCCCGG 20 1366

HSV2-UL19-469 - GCCGACGUCGAGCUUCCCGG 20 709

HSV2-UL19-1406 + AUCGCCCCCGGACCGGCCGG 20 1646

HSV2-UL19-675 - CCGCGGGGACGCGCGGCCGG 20 915

HSV2-UL19-1248 + UAGUUGGCCCCCAGGGCCGG 20 1488

HSV2-UL19-1260 + GCCAGGUCGCGCAGGGCCGG 20 1500

HSV2-UL19-1460 + GCGGGGCUCCGGGUGGCCGG 20 1700

HSV2-UL19-475 - AGUCCCCCCGGCCGGUCCGG 20 715

HSV2-UL19-460 - UCGCCCACGGCCGGGUCCGG 20 700

HSV2-UL19-482 - GCCCCUGGCGCUGUGUCCGG 20 722

HSV2-UL19-1191 + GCCGCGUUGUCUAGCAGCGG 20 1431

HSV2-UL19-1130 + CUGCACGUCGCUGGCAGCGG 20 1370

HSV2-UL19-536 - AGACUGCCGGAUUGACGCGG 20 776

HSV2-UL19-540 - GCACGAGCCCGUCUACGCGG 20 780

HSV2-UL19-679 - GGCCGGCGGCGUGUACGCGG 20 919

HSV2-UL19-560 - GGGGCGCUGCUGCACCGCGG 20 800

HSV2-UL19-601 - GCACGCCCGCGAGAGCGCGG 20 841

HSV2-UL19-556 - UGGUGCCGGCCUUCUCGCGG 20 796

HSV2-UL19-604 - CGCCCGCGAGAGCGCGGCGG 20 844 HSV2-UL19-1512 + GCGCUGUUUGAUGGCGGCGG 20 1752

HSV2-UL19-629 - GCCCGCCACGAAACGGGCGG 20 869

HSV2-UL19-1511 + GAGGCGCUGUUUGAUGGCGG 20 1751

HSV2-UL19-709 - CAUCGUGGAAACGGGAUCGG 20 949

HSV2-UL19-506 - UACAUCGUGACCUACCUCGG 20 746

HSV2-UL19-1342 + CGUCAAUCCGGCAGUCUCGG 20 1582

HSV2-UL19-444 - AACAUCGACGCGGCCGUCGG 20 684

HSV2-UL19-619 - GUUUUCCGAGCGCGCGUCGG 20 859

HSV2-UL19-1329 + CCGGUGCGGCCGGUCGUCGG 20 1569

HSV2-UL19-1113 + GCGGGCGCUGCGUAGCAGGG 20 1353

HSV2-UL19-626 - GUGGCCCGCCACGAAACGGG 20 866

HSV2-UL19-1178 + GGGCGCAGCCAAAGACCGGG 20 1418

HSV2-UL19-1407 + UCGCCCCCGGACCGGCCGGG 20 1647

HSV2-UL19-1249 + AGUUGGCCCCCAGGGCCGGG 20 1489

HSV2-UL19-1261 + CCAGGUCGCGCAGGGCCGGG 20 1501

HSV2-UL19-1456 + GGAACGCGCGGGGCUCCGGG 20 1696

HSV2-UL19-1192 + CCGCGUUGUCUAGCAGCGGG 20 1432

HSV2-UL19-1298 + GAU UGGCGGGAUGCAGCGGG 20 1538

HSV2-UL19-537 - GACUGCCGGAUUGACGCGGG 20 777

HSV2-UL19-373 - CGCACGCCGACACGCGCGGG 20 613

HSV2-UL19-630 - CCCGCCACGAAACGGGCGGG 20 870

HSV2-UL19-1543 + G G CAACAG CAG G G CCAG GG G 20 1783

HSV2-UL19-1350 + UCGCAGUCCCACACGAGGGG 20 1590

HSV2-UL19-1225 + CGGUGAACCCGAACCCGGGG 20 1465

HSV2-UL19-1449 + CAAAAAAACAG CU G CCG G G G 20 1689

HSV2-UL19-1193 + CGCGUUGUCUAGCAGCGGGG 20 1433

HSV2-UL19-1104 + AGACAGGCCCU UUAGCGGGG 20 1344

HSV2-UL19-538 - ACUGCCGGAU UGACGCGGGG 20 778

HSV2-UL19-631 - CCGCCACGAAACGGGCGGGG 20 871

HSV2-UL19-1371 + CGGCCAUGCACUCCUCGGGG 20 1611

HSV2-UL19-1169 + GAUGUCCGUGGCCACGGGGG 20 1409

HSV2-UL19-1461 + GGGCUCCGGGUGGCCGGGGG 20 1701

HSV2-UL19-1200 + CGAGGUAAAAGUUUUGGGGG 20 1440

HSV2-UL19-720 - ACGCAGCGCCCGCGAUGGGG 20 960

HSV2-UL19-522 - CUGCCGGGCCCGGAGCUGGG 20 762

HSV2-UL19-1279 + GGUGUGGUCCAGGUGCUGGG 20 1519

HSV2-UL19-1492 + GCGCACGCGCGUUGUCUGGG 20 1732

HSV2-UL19-1300 + GGCGGGAUGCAGCGGGUGGG 20 1540

HSV2-UL19-562 - CGCCACGCUGCAGAACAUGG 20 802

HSV2-UL19-1289 + CACCUGCAGCGUGAGCAUGG 20 1529

HSV2-UL19-606 - GCUGACCUACGCGCUCAUGG 20 846

HSV2-UL19-1241 + CACCACGGGCUGGCGGAUGG 20 1481

HSV2-UL19-1510 + CAGGAGGCGCUGUUUGAUGG 20 1750

HSV2-UL19-1445 + UGGACAGCCGCAGAACCUGG 20 1685

HSV2-UL19-512 - GGCCGUGUAUCGGGACCUGG 20 752

HSV2-UL19-415 - CAACGUGCCCUACCCCCUGG 20 655 HSV2-UL19-598 - CCUGGUCCCCCCGGCCCUGG 20 838

HSV2-UL19-455 - AGCGUUUUCUGAACGCCUGG 20 695

HSV2-UL19-345 - CAACGUCCAGGCCGUCCUGG 20 585

HSV2-UL19-412 - CGACAGGCUGGUCUUCCUGG 20 652

HSV2-UL19-516 - CGAGGCCCUGGCCCAGCUGG 20 756

HSV2-UL19-1239 + CGUGCUGCACCACGGGCUGG 20 1479

HSV2-UL19-1278 + UGGUGUGGUCCAGGUGCUGG 20 1518

HSV2-UL19-567 - GCUGCAUCCCGCCAAUCUGG 20 807

HSV2-UL19-407 - GCGCGUGCGCGCGGAUCUGG 20 647

HSV2-UL19-1491 + CGCGCACGCGCGUUGUCUGG 20 1731

HSV2-UL19-1389 + UAGCCGGCGCCAUGGCGUGG 20 1629

HSV2-UL19-651 - CCUGCGCAACGCGGUCGUGG 20 891

HSV2-UL19-1214 + CCCCCCCGCCCGUUUCGUGG 20 1454

HSV2-UL19-381 - GCAGUCCUUCCUGAAGGUGG 20 621

HSV2-UL19-1496 + CCUGCAUAUCCAGCAGGUGG 20 1736

HSV2-UL19-1343 + CAAUCCGGCAGUCUCGGUGG 20 1583

HSV2-UL19-1226 + UGAACCCGAACCCGGGGUGG 20 1466

HSV2-UL19-1483 + GGGGUAGGGCACGUUGGUGG 20 1723

HSV2-UL19-1321 + CGUAAUAGUAGAUUUUGUGG 20 1561

HSV2-UL19-1294 + CGUGU UGGCCACCAGAUUGG 20 1534

HSV2-UL19-1482 + CAGGGGGUAGGGCACGUUGG 20 1722

HSV2-UL19-1199 + GGCCGAGGUAAAAGUUUUGG 20 1439

HSV2-UL19-1156 + UCUGGCCGUGGUCGUACAUG 20 1396

HSV2-UL19-719 - GCUACGCAGCGCCCGCGAUG 20 959

HSV2-UL19-420 - UGACGUUCGUCCUGCCCCUG 20 660

HSV2-UL19-597 - CCCUGGUCCCCCCGGCCCUG 20 837

HSV2-UL19-3077 - GCAACGUCCAGGCCGUCCUG 20 7460

HSV2-UL19-1381 + AAAACACCGCCGGGUAGCUG 20 1621

HSV2-UL19-656 - UCGUGGCGGGAAACCGGCUG 20 896

HSV2-UL19-1139 + AGAACUUGAAGCAGGGGCUG 20 1379

HSV2-UL19-1277 + AUGGUGUGGUCCAGGUGCUG 20 1517

HSV2-UL19-1490 + GCGCGCACGCGCGUUGUCUG 20 1730

HSV2-UL19-435 - GAAAGGACCACCAGGUUCUG 20 675

HSV2-UL19-715 - GAGCUCGUGGAAGACCCGUG 20 955

HSV2-UL19-394 - UGGUGAUGGGCAAGGCCGUG 20 634

HSV2-UL19-527 - CGCUGCUGCCGCCCCUCGUG 20 767

HSV2-UL19-616 - GGUUCGGGUUCACCGUCGUG 20 856

HSV2-UL19-565 - CCCGGAGAUCGCCCCCGGUG 20 805

HSV2-UL19-1326 + GUCCAGUCGGCCGGCCGGUG 20 1566

HSV2-UL19-636 - CGGAAACGUGGACCUGGGUG 20 876

HSV2-UL19-1273 + AUUCGCCAUUUUGGAUGGUG 20 1513

HSV2-UL19-1143 + GGACGGGCGAGGCCCCGUUG 20 1383

HSV2-UL19-1291 + CGACGACGACGCGCCCGUUG 20 1531

HSV2-UL19-1336 + GCAGCAGCCGGCCGUCGUUG 20 1576

HSV2-UL19-1204 + CCAUGUCCGUAACGGGGUUG 20 1444

HSV2-UL19-588 - GCCCGUCCACGCGCUGUUUG 20 828 HSV2-UL19-1320 + GCACGUAAUAGUAGAUUUUG 20 1560

HSV2-UL19-1198 + CGGCCGAGGUAAAAGUUUUG 20 1438

HSV2-UL19-432 - CCCCGGCAGCUGUUU UUUUG 20 672

HSV2-UL19-3104 - AGGGCCUGUCUCUGUAAAAU 20 7487

HSV2-UL19-643 - CGCAACCCCGUUACGGACAU 20 883

HSV2-UL19-408 - CGCGCGGAUCUGGUGGCCAU 20 648

HSV2-UL19-1537 + UUGUCGAGAUAUCGUUGCAU 20 1777

HSV2-UL19-1293 + GACCGUGUUGGCCACCAGAU 20 1533

HSV2-UL19-718 - UGCUACGCAGCGCCCGCGAU 20 958

HSV2-UL19-708 - UCUCAUCGUGGAAACGGGAU 20 948

HSV2-UL19-1118 + GGGUCGCUGGCGCAGGUGAU 20 1358

HSV2-UL19-1144 + CGAGGCCCCGUUGAGGUGAU 20 1384

HSV2-UL19-392 - CUCGUCACGGCGCUGGUGAU 20 632

HSV2-UL19-509 - AGGAGUGCAUGGCCGUGUAU 20 749

HSV2-UL19-1420 + CUCCGCGGUCAUCUGGCACU 20 1660

HSV2-UL19-1175 + GCCGGCGCGCCGCGGCACCU 20 1415

HSV2-UL19-635 - CCGCGCGGAAACGUGGACCU 20 875

HSV2-UL19-503 - UCGUACAUCGUGACCUACCU 20 743

HSV2-UL19-644 - CU CCCCCAAAACU U U U ACCU 20 884

HSV2-UL19-419 - CUGACGUUCGUCCUGCCCCU 20 659

HSV2-UL19-596 - CCCCUGGUCCCCCCGGCCCU 20 836

HSV2-UL19-1504 + GGUCACCGGCACGUCGGCCU 20 1744

HSV2-UL19-1334 + GGCGUCGGCCGCGCGGGCCU 20 1574

HSV2-UL19-1368 + AUACACGGCCAUGCACUCCU 20 1608

HSV2-UL19-3085 - CGCAACGUCCAGGCCGUCCU 20 7468

HSV2-UL19-361 - UGCGACACGAGCUUCUUCCU 20 601

HSV2-UL19-1361 + GGUAAAGUCGUCCACCAGCU 20 1601

HSV2-UL19-1354 + GCGCAUCAGGUGAUUCAGCU 20 1594

HSV2-UL19-1529 + GCAAAAGCUCCGCUUCAGCU 20 1769

HSV2-UL19-521 - ACCCUGCCGGGCCCGGAGCU 20 761

HSV2-UL19-487 - GACGCCCGGGGCCUGGAGCU 20 727

HSV2-UL19-1218 + GUACGCCUCCGACGCGCGCU 20 1458

HSV2-UL19-1287 + CAGCAGCGCCGUCGUGCGCU 20 1527

HSV2-UL19-1306 + GGGUCGGUCACGGGAUCGCU 20 1546

HSV2-UL19-655 - GUCGUGGCGGGAAACCGGCU 20 895

HSV2-UL19-1212 + GUCCACGUUUCCGCGCGGCU 20 1452

HSV2-UL19-1182 + GCCAAAGACCGGGAGGGGCU 20 1422

HSV2-UL19-1276 + GAUGGUGUGGUCCAGGUGCU 20 1516

HSV2-UL19-1341 + CCGCGUCAAUCCGGCAGUCU 20 1581

HSV2-UL19-1489 + CGCGCGCACGCGCGUUGUCU 20 1729

HSV2-UL19-1219 + CUCGGAAAACAGCACGUUCU 20 1459

HSV2-UL19-621 - GCGUCGGAGGCGUACUUUCU 20 861

HSV2-UL19-1106 + GAUGAGAUACUGCGUAAAGU 20 1346

HSV2-UL19-1337 + GCCGUCGUUGCGGUUAAAGU 20 1577

HSV2-UL19-1262 + GCGCAGGGCCGGGGGGAAGU 20 1502

HSV2-UL19-1323 + UUUGUGGUGGACGGUCCAGU 20 1563 HSV2-UL19-1242 + GCGGAUGGAGGAGAAGUAGU 20 1482

HSV2-UL19-1503 + GCCGUAGGUCACCGGCACGU 20 1743

HSV2-UL19-1481 + CACCAGGGGGUAGGGCACGU 20 1721

HSV2-UL19-397 - GUGCGGAGCCUGGACGACGU 20 637

HSV2-UL19-1365 + CUGGGCCAGGGCCUCGACGU 20 1605

HSV2-UL19-1410 + GCCGCCGGGAAGCUCGACGU 20 1650

HSV2-UL19-691 - CGCGGCCACGGCCAACCCGU 20 931

HSV2-UL19-1501 + GUUCAAGACCAUCUCGCCGU 20 1741

HSV2-UL19-1424 + GACCCACCGGACCCGGCCGU 20 1664

HSV2-UL19-441 - AUGAACAUCGACGCGGCCGU 20 681

HSV2-UL19-1284 + CGCGGUGUUGGCGCCCGCGU 20 1524

HSV2-UL19-1233 + GUACCCCGCCAUGAGCGCGU 20 1473

HSV2-UL19-618 - GCUGUUUUCCGAGCGCGCGU 20 858

HSV2-UL19-1108 + AAAGUGGGUCUCUCGCGCGU 20 1348

HSV2-UL19-1264 + CGGGGGGAAGUUGGGCGCGU 20 1504

HSV2-UL19-1330 + CGGCCGGUCGUCGGCGGCGU 20 1570

HSV2-UL19-488 - CGGGGCCUGGAGCUCGGCGU 20 728

HSV2-UL19-1328 + CGGCCGGUGCGGCCGGUCGU 20 1568

HSV2-UL19-1415 + GUGCAGCUCCAGAGCCAGGU 20 1655

HSV2-UL19-1500 + CACCAGCGCCGUGACGAGGU 20 1740

HSV2-UL19-1373 + GGGGAGGUCGCCCCCGAGGU 20 1613

HSV2-UL19-461 - CGCCCACGGCCGGGUCCGGU 20 701

HSV2-UL19-438 - CUGUCCAUGGAGAACGCGGU 20 678

HSV2-UL19-1148 + CCGCUGCGACGCCCACGGGU 20 1388

HSV2-UL19-1299 + AUUGGCGGGAUGCAGCGGGU 20 1539

HSV2-UL19-1120 + GCUGGCGCAGGUGAUCGGGU 20 1360

HSV2-UL19-1303 + AUGCAGCGGGUGGGCGGGGU 20 1543

HSV2-UL19-1479 + CAUGGCGCCCACCAGGGGGU 20 1719

HSV2-UL19-416 - AACGUGCCCUACCCCCUGGU 20 656

HSV2-UL19-1545 + CAGCACGUGCAGCAUCUGGU 20 1785

HSV2-UL19-1266 + GGGCGCGUUGGCCACGUGGU 20 1506

HSV2-UL19-1281 + CCCGUCGAAGAUGCGCAUGU 20 1521

HSV2-UL19-1134 + GCGAUGUUUGGCCGUGAUGU 20 1374

HSV2-UL19-348 - CCAGAUGCUGCACGUGCUGU 20 588

HSV2-UL19-1292 + GAACAUGCGCUUGACCGUGU 20 1532

HSV2-UL19-1514 + GACCGGCCGCCCGCGCGUGU 20 1754

HSV2-UL19-528 - GCUGCUGCCGCCCCUCGUGU 20 768

HSV2-UL19-1283 + GUUGGCCGUCGACGCGGUGU 20 1523

HSV2-UL19-637 - GGAAACGUGGACCUGGGUGU 20 877

HSV2-UL19-1416 + CAGAGCCAGGUUGGCGUUGU 20 1656

HSV2-UL19-659 - GCCCAGCCCCUCCCGGUCUU 20 899

HSV2-UL19-1263 + CGCAGGGCCGGGGGGAAGUU 20 1503

HSV2-UL19-614 - ACGGGCCUCCACCCCGGGUU 20 854

HSV2-UL19-1133 + ACGCUCCAGGCAGCGAUGUU 20 1373

HSV2-UL19-1271 + CGUAGAAGUAUUCGCCAUUU 20 1511

HSV2-UL19-1196 + CGCGGCCGAGGUAAAAGUUU 20 1436 HSV2-UL19-1197 + GCGGCCGAGGUAAAAGUUUU 20 1437

HSV2-UL19-430 - CUCCCCGGCAGCUGUUUUUU 20 670

HSV2-UL19-431 - UCCCCGGCAGCUGUUUUUUU 20 671

Table 6 A provides exemplary targeting domains for knocking out the UL19 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality, and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 6A

HSV2-UL19-3129 - GCCGCUCCUGCCCGCGACCCC 21 7512

HSV2-UL19-3130 - GGCCGCUCCUGCCCGCGACCCC 22 7513

HSV2-UL19-3131 - GUAGAGU UUGACGCCCUGC 19 7514

HSV2-UL19-3132 - GACGUAGAGUU UGACGCCCUGC 22 7515

HSV2-U L19-3133 - GCCAUCCUGCCCACCGGCUCCAUC 24 7516

HSV2-U L19-3134 - GAAAACAGCCUGUAUGACG 19 7517

HSV2-UL19-3135 - GACGAAAACAGCCUGUAUGACG 22 7518

Table 6B provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 6B

HSV2-UL19-3154 + CGUACUCAGGAUGGAGCCGGUGGG 24 7537

HSV2-UL19-3155 + UGGACGUUGCGCGCGAGCUG 20 7538

HSV2-UL19-3156 + CUGGACGUUGCGCGCGAGCUG 21 7539

HSV2-UL19-3157 + CCUGGACGU UGCGCGCGAGCUG 22 7540

HSV2-UL19-3158 + CGGCCAUUGGGUUCCU UG 18 7541

HSV2-UL19-3083 + AGCGGCCAUUGGGUUCCUUG 20 7466

HSV2-UL19-3159 + AGGAGCGGCCAUUGGGUUCCUUG 23 7542

HSV2-UL19-3160 + CAGGAGCGGCCAUUGGG UUCCUUG 24 7543

HSV2-UL19-3161 + CGCCACCUCGAUCGUACU 18 7544

HSV2-UL19-3162 + ACGCCACCUCGAUCGUACU 19 7545

HSV2-UL19-3163 + UGGGACGCCACCUCGAUCGUACU 23 7546

HSV2-UL19-3164 - CGCUCCUGCCCGCGACCCC 19 7547

HSV2-UL19-199 - CCGCUCCUGCCCGCGACCCC 20 2237

HSV2-UL19-3165 - UGGCCGCUCCUGCCCGCGACCCC 23 7548

HSV2-UL19-3166 - AUGGCCGCUCCUGCCCGCGACCCC 24 7549

HSV2-UL19-3167 - UAGAGUU UGACGCCCUGC 18 7550

HSV2-UL19-8 - CGUAGAGUUUGACGCCCUGC 20 389

HSV2-UL19-3168 - ACGUAGAGU UUGACGCCCUGC 21 7551

HSV2-UL19-3169 - UGACGUAGAGUUUGACGCCCUGC 23 7552

HSV2-UL19-3170 - AUGACGUAGAGUUUGACGCCCUGC 24 7553

HSV2-UL19-3171 - AUCCUGCCCACCGGCUCCAUC 21 7554

HSV2-UL19-3172 - CAUCCUGCCCACCGGCUCCAUC 22 7555

HSV2-UL19-3173 - CCAUCCUGCCCACCGGCUCCAUC 23 7556

HSV2-UL19-3174 - AAAACAGCCUGUAUGACG 18 7557

HSV2-UL19-203 - CGAAAACAGCCUGUAUGACG 20 2241

HSV2-UL19-3175 - ACG AAAACAGCCUGUAUGACG 21 7558

HSV2-UL19-3176 - CGACG AAAACAGCCUGUAUGACG 23 7559

HSV2-UL19-3177 - CCG ACG AAAACAGCCUG U AUG ACG 24 7560

Table 6C provides exemplary targeting domains for knocking out the UL19 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 6C

HSV2-U L19-3178 + CGCGGGCAGGAGCGGCCA 18 7561

HSV2-U L19-3179 + UCGCGGGCAGGAGCGGCCA 19 7562

HSV2-U L19-3180 + G UCGCGGGCAGGAGCGGCCA 20 7563

HSV2-U L19-3181 + CAGGAUGGAGCCGG UGGG 18 7564

HSV2-U L19-3182 + UCAGGAUGGAGCCGGUGGG 19 7565

HSV2-U L19-292 + CUCAGGAUGGAGCCGG UGGG 20 2307

HSV2-U L19-3183 - CUGCCCACCGGCUCCAUC 18 7566

HSV2-U L19-3184 - CCUGCCCACCGGCUCCAUC 19 7567

HSV2-U L19-200 - UCCUGCCCACCGGCUCCAUC 20 2238

Table 6D provides exemplary targeting domains for knocking out the UL19 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 6D

HSV2-UL19-3201 + AUGGCCGCGGCGUACCGGUAACC 23 7584

HSV2-UL19-3202 + GAUGGCCGCGGCGUACCGGUAACC 24 7585

HSV2-UL19-3203 + AUGAGGGGCUGGUGGACC 18 7586

HSV2-UL19-3204 + GAUGAGGGGCUGGUGGACC 19 7587

HSV2-UL19-271 + CGAUGAGGGGCUGGUGGACC 20 2291

HSV2-UL19-3205 + GCGAUGAGGGGCUGGUGGACC 21 7588

HSV2-UL19-3206 + GGCG AUGAGGGGCUGGUGGACC 22 7589

HSV2-UL19-3207 + GGGCGAUGAGGGGCUGGUGGACC 23 7590

HSV2-UL19-3208 + CGGGCGAUGAGGGGCUGGUGGACC 24 7591

HSV2-UL19-3209 + GCGGCGUACCGGUAACCC 18 7592

HSV2-UL19-3210 + CGCGGCGUACCGGUAACCC 19 7593

HSV2-UL19-92 + CCGCGGCGUACCGGUAACCC 20 507

HSV2-UL19-3211 + GCCGCGGCGUACCGGUAACCC 21 7594

HSV2-UL19-3212 + GGCCGCGGCGUACCGGUAACCC 22 7595

HSV2-UL19-3213 + UGGCCGCGGCGUACCGGUAACCC 23 7596

HSV2-UL19-3214 + AUGGCCGCGGCGUACCGGUAACCC 24 7597

HSV2-UL19-3215 + CCGUCCAGGGCCUCCCCC 18 7598

HSV2-UL19-3216 + CCCGUCCAGGGCCUCCCCC 19 7599

HSV2-UL19-258 + UCCCGUCCAGGGCCUCCCCC 20 2280

HSV2-UL19-3217 + GUCCCGUCCAGGGCCUCCCCC 21 7600

HSV2-UL19-3218 + CGUCCCGUCCAGGGCCUCCCCC 22 7601

HSV2-UL19-3219 + CCGUCCCGUCCAGGGCCUCCCCC 23 7602

HSV2-UL19-3220 + CCCGUCCCGUCCAGGGCCUCCCCC 24 7603

HSV2-UL19-3221 + UUGGUGCACACGCACGCC 18 7604

HSV2-UL19-3222 + CUUGGUGCACACGCACGCC 19 7605

HSV2-UL19-275 + ACUUGGUGCACACGCACGCC 20 2293

HSV2-UL19-3223 + AACUUGGUGCACACGCACGCC 21 7606

HSV2-UL19-3224 + GAACUUGGUG CACACG CACG CC 22 7607

HSV2-UL19-3225 + GGAACUUGGUGCACACGCACGCC 23 7608

HSV2-UL19-3226 + GGGAACUUGGUGCACACGCACGCC 24 7609

HSV2-UL19-3227 + UUCAUGUAAGCCAGCUCC 18 7610

HSV2-UL19-3228 + GUUCAUGUAAGCCAGCUCC 19 7611

HSV2-UL19-66 + CGUUCAUGUAAGCCAGCUCC 20 406

HSV2-UL19-3229 + UCGUUCAUGUAAGCCAGCUCC 21 7612

HSV2-UL19-3230 + UUCGUUCAUGUAAGCCAGCUCC 22 7613

HSV2-UL19-3231 + CUUCGUUCAUGUAAGCCAGCUCC 23 7614

HSV2-UL19-3232 + CCUUCGUUCAUGUAAGCCAGCUCC 24 7615

HSV2-UL19-3233 + GAGCGGCCAU UGGGUUCC 18 7616

HSV2-UL19-3234 + GGAGCGGCCAUUGGGUUCC 19 7617

HSV2-UL19-3235 + AGGAGCGGCCAUUGGGUUCC 20 7618

HSV2-UL19-3236 + CAGGAGCGGCCAUUGGGUUCC 21 7619

HSV2-UL19-3237 + GCAGGAGCGGCCAUUGGGUUCC 22 7620

HSV2-UL19-3238 + GGCAGGAGCGGCCAUUGGGUUCC 23 7621

HSV2-UL19-3239 + GGGCAGGAGCGGCCAUUGGGUUCC 24 7622

HSV2-UL19-3240 + CCGAGCUCCAGAAAGCGC 18 7623

HSV2-UL19-3241 + GCCGAGCUCCAGAAAGCGC 19 7624 HSV2-UL19-278 + GGCCGAGCUCCAGAAAGCGC 20 2296

HSV2-UL19-3242 + AGGCCGAGCUCCAGAAAGCGC 21 7625

HSV2-UL19-3243 + CAGGCCGAGCUCCAGAAAGCGC 22 7626

HSV2-UL19-3244 + ACAGGCCGAGCUCCAGAAAGCGC 23 7627

HSV2-UL19-3245 + GACAGGCCGAGCUCCAGAAAGCGC 24 7628

HSV2-UL19-3246 + ACGGCCUGGACGUUGCGC 18 7629

HSV2-UL19-3247 + GACGGCCUGGACGUUGCGC 19 7630

HSV2-UL19-3248 + GG ACGGCCUGGACGUUGCGC 20 7631

HSV2-UL19-3249 + AGG ACGGCCUGGACGUUGCGC 21 7632

HSV2-UL19-3250 + CAGG ACGGCCUGGACGUUGCGC 22 7633

HSV2-UL19-3251 + CCAGGACGGCCUGGACGUUGCGC 23 7634

HSV2-UL19-3252 + CCCAGGACGGCCUGGACGUUGCGC 24 7635

HSV2-UL19-3253 + AAACGCCCCCAGGACGGC 18 7636

HSV2-UL19-3254 + CAAACGCCCCCAGGACGGC 19 7637

HSV2-UL19-2781 + UCAAACGCCCCCAGGACGGC 20 2863

HSV2-UL19-3255 + ACCCGGGGGGUCGCGGGC 18 7638

HSV2-UL19-3256 + AACCCGGGGGGUCGCGGGC 19 7639

HSV2-UL19-98 + UAACCCGGGGGGUCGCGGGC 20 413

HSV2-UL19-3257 + GUAACCCGGGGGGUCGCGGGC 21 7640

HSV2-UL19-3258 + GG UAACCCGGGGGGUCGCGGGC 22 7641

HSV2-UL19-3259 + CGGU AACCCGGGGGGUCGCGGGC 23 7642

HSV2-UL19-3260 + CCGGUAACCCGGGGGGUCGCGGGC 24 7643

HSV2-UL19-3261 + UUCGUCAUGUAAUUAUGC 18 7644

HSV2-UL19-3262 + CUUCGUCAUGUAAUUAUGC 19 7645

HSV2-UL19-262 + CCUUCGUCAUGUAAUUAUGC 20 2284

HSV2-UL19-3263 + ACCUUCGUCAUGUAAUUAUGC 21 7646

HSV2-UL19-3264 + GACCUUCGUCAUGUAAUUAUGC 22 7647

HSV2-UL19-3265 + UGACCUUCGUCAUGUAAUUAUGC 23 7648

HSV2-UL19-3266 + AUGACCUUCGUCAUGUAAUUAUGC 24 7649

HSV2-UL19-3267 + GUUCAUGUAAGCCAGCUC 18 7650

HSV2-UL19-3268 + CGUUCAUGUAAGCCAGCUC 19 7651

HSV2-UL19-65 + UCGUUCAUGUAAGCCAGCUC 20 405

HSV2-UL19-3269 + UUCGUUCAUGUAAGCCAGCUC 21 7652

HSV2-UL19-3270 + CUUCGU UCAUGUAAGCCAGCUC 22 7653

HSV2-UL19-3271 + CCUUCGUUCAUGUAAGCCAGCUC 23 7654

HSV2-UL19-3272 + CCCUUCGUUCAUGUAAGCCAGCUC 24 7655

HSV2-UL19-3273 + CACGGACAGGCCGAGCUC 18 7656

HSV2-UL19-3274 + CCACGGACAGGCCGAGCUC 19 7657

HSV2-UL19-277 + GCCACGGACAGGCCGAGCUC 20 2295

HSV2-UL19-3275 + CGCCACGGACAGGCCGAGCUC 21 7658

HSV2-UL19-3276 + ACGCCACGGACAGGCCGAGCUC 22 7659

HSV2-UL19-3277 + CACGCCACGGACAGGCCGAGCUC 23 7660

HSV2-UL19-3278 + GCACGCCACGGACAGGCCGAGCUC 24 7661

HSV2-UL19-3279 + GCUAAUGCCCGUCCCGUC 18 7662

HSV2-UL19-3280 + GGCUAAUGCCCGUCCCGUC 19 7663

HSV2-UL19-257 + AGGCUAAUGCCCGUCCCGUC 20 2279 HSV2-UL19-3281 + CAGGCUAAUGCCCGUCCCGUC 21 7664

HSV2-UL19-3282 + GCAGGCUAAUGCCCGUCCCGUC 22 7665

HSV2-UL19-3283 + UGCAGGCUAAUGCCCGUCCCGUC 23 7666

HSV2-UL19-3284 + AUGCAGGCUAAUGCCCGUCCCGUC 24 7667

HSV2-UL19-3285 + GACGGGGUGCGGGCCGUC 18 7668

HSV2-UL19-3286 + CGACGGGGUGCGGGCCGUC 19 7669

HSV2-UL19-266 + UCGACGGGGUGCGGGCCGUC 20 2287

HSV2-UL19-3287 + CUCGACGGGGUGCGGGCCGUC 21 7670

HSV2-UL19-3288 + GCUCGACGGGGUGCGGGCCGUC 22 7671

HSV2-UL19-3289 + UGCUCGACGGGGUGCGGGCCGUC 23 7672

HSV2-UL19-3290 + CUGCUCGACGGGGUGCGGGCCGUC 24 7673

HSV2-UL19-3291 + CCGGUAACCCGGGGGGUC 18 7674

HSV2-UL19-3292 + ACCGGUAACCCGGGGGGUC 19 7675

HSV2-UL19-297 + UACCGGUAACCCGGGGGGUC 20 2309

HSV2-UL19-3293 + GUACCGGUAACCCGGGGGGUC 21 7676

HSV2-UL19-3294 + CGU ACCGGUAACCCGGGGGGUC 22 7677

HSV2-UL19-3295 + GCGU ACCGGUAACCCGGGGGGUC 23 7678

HSV2-UL19-3296 + GGCGUACCGGUAACCCGGGGGGUC 24 7679

HSV2-UL19-3297 + GUUGCAGUAGGACCCCAG 18 7680

HSV2-UL19-3298 + UGUUGCAGUAGGACCCCAG 19 7681

HSV2-UL19-281 + GUGU UGCAGUAGGACCCCAG 20 2299

HSV2-UL19-3299 + GGUGUUGCAGUAGGACCCCAG 21 7682

HSV2-UL19-3300 + GGG UGUUGCAGUAGGACCCCAG 22 7683

HSV2-UL19-3301 + AGGG UGUUGCAGUAGGACCCCAG 23 7684

HSV2-UL19-3302 + CAGGGUGUUGCAGUAGGACCCCAG 24 7685

HSV2-UL19-3303 + AGCGACAGGGUGUUGCAG 18 7686

HSV2-UL19-3304 + GAGCGACAGGGUGUUGCAG 19 7687

HSV2-UL19-280 + CGAGCGACAGGGUGUUGCAG 20 2298

HSV2-UL19-3305 + ACGAGCGACAGGGUGUUGCAG 21 7688

HSV2-UL19-3306 + CACGAGCGACAGGGUGUUGCAG 22 7689

HSV2-UL19-3307 + GCACGAGCGACAGGGUGUUGCAG 23 7690

HSV2-UL19-3308 + CGCACGAGCGACAGGGUGUUGCAG 24 7691

HSV2-UL19-3309 + CAGGGCCUCCCCCGUGAG 18 7692

HSV2-UL19-3310 + CCAGGGCCUCCCCCGUGAG 19 7693

HSV2-UL19-259 + UCCAGGGCCUCCCCCGUGAG 20 2281

HSV2-UL19-3311 + GUCCAGGGCCUCCCCCGUGAG 21 7694

HSV2-UL19-3312 + CGUCCAGGGCCUCCCCCGUGAG 22 7695

HSV2-UL19-3313 + CCGUCCAGGGCCUCCCCCGUGAG 23 7696

HSV2-UL19-3314 + CCCGUCCAGGGCCUCCCCCGUGAG 24 7697

HSV2-UL19-3315 + GUACUCAGGAUGGAGCCG 18 7698

HSV2-UL19-3316 + CGUACUCAGGAUGGAGCCG 19 7699

HSV2-UL19-291 + UCGUACUCAGGAUGGAGCCG 20 2306

HSV2-UL19-3317 + AUCGUACUCAGGAUGGAGCCG 21 7700

HSV2-UL19-3318 + GAU CGUACUCAGGAUGGAGCCG 22 7701

HSV2-UL19-3319 + CG AUCGUACUCAGGAUGGAGCCG 23 7702

HSV2-UL19-3320 + UCG AUCGUACUCAGGAUGGAGCCG 24 7703 HSV2-UL19-3321 + UCGUCGGAGCGCACGGCG 18 7704

HSV2-UL19-3322 + UUCGUCGGAGCGCACGGCG 19 7705

HSV2-UL19-284 + UU UCGUCGGAGCGCACGGCG 20 2301

HSV2-UL19-3323 + UUUUCGUCGGAGCGCACGGCG 21 7706

HSV2-UL19-3324 + GUU UUCGUCGGAGCGCACGGCG 22 7707

HSV2-UL19-3325 + UGUUUUCGUCGGAGCGCACGGCG 23 7708

HSV2-UL19-3326 + CUGU UUUCGUCGGAGCGCACGGCG 24 7709

HSV2-UL19-3327 + UGCGGGCCGUCGCGGGCG 18 7710

HSV2-UL19-3328 + GUGCGGGCCGUCGCGGGCG 19 7711

HSV2-UL19-267 + GGUGCGGGCCGUCGCGGGCG 20 2288

HSV2-UL19-3329 + GGGUGCGGGCCGUCGCGGGCG 21 7712

HSV2-UL19-3330 + GGGGUGCGGGCCGUCGCGGGCG 22 7713

HSV2-UL19-3331 + CGGGGUGCGGGCCGUCGCGGGCG 23 7714

HSV2-UL19-3332 + ACGGGGUGCGGGCCGUCGCGGGCG 24 7715

HSV2-UL19-3333 + AAAAU CAAAG AG U CU GCG 18 7716

HSV2-UL19-3334 + AAAAAUCAAAGAGUCUGCG 19 7717

HSV2-UL19-286 + AAAAAAU CAAAG AG U CU GCG 20 2303

HSV2-UL19-3335 + GAAAAAAUCAAAGAGUCUGCG 21 7718

HSV2-UL19-3336 + CGAAAAAAUCAAAGAGUCUGCG 22 7719

HSV2-UL19-3337 + GCG AAAAAAU CAAAG AG U CUG CG 23 7720

HSV2-UL19-3338 + GGCGAAAAAAUCAAAGAGUCUGCG 24 7721

HSV2-UL19-3339 + UUAUGCACGGGCUGCUCG 18 7722

HSV2-UL19-3340 + AUUAUGCACGGGCUGCUCG 19 7723

HSV2-UL19-263 + AAU UAUGCACGGGCUGCUCG 20 2285

HSV2-UL19-3341 + UAAUUAUGCACGGGCUGCUCG 21 7724

HSV2-UL19-3342 + GUAAUUAUGCACGGGCUGCUCG 22 7725

HSV2-UL19-3343 + UGUAAUUAUGCACGGGCUGCUCG 23 7726

HSV2-UL19-3344 + AUGUAAUUAUGCACGGGCUGCUCG 24 7727

HSV2-UL19-3345 + UCAUACAGGCUGUUUUCG 18 7728

HSV2-UL19-3346 + GUCAUACAGGCUGUUUUCG 19 7729

HSV2-UL19-282 + CGUCAUACAGGCUGUUUUCG 20 2300

HSV2-UL19-3347 + ACGUCAUACAGGCUGUUUUCG 21 7730

HSV2-UL19-3348 + UACGUCAUACAGGCUGUUUUCG 22 7731

HSV2-UL19-3349 + CUACGUCAUACAGGCUGUUUUCG 23 7732

HSV2-UL19-3350 + UCUACGUCAUACAGGCUGUUUUCG 24 7733

HSV2-UL19-3351 + ACGCACGCCACGGACAGG 18 7734

HSV2-UL19-3352 + CACGCACGCCACGGACAGG 19 7735

HSV2-UL19-276 + ACACGCACGCCACGGACAGG 20 2294

HSV2-UL19-3353 + CACACGCACGCCACGGACAGG 21 7736

HSV2-UL19-3354 + G CACACG CACG CCACG G ACAG G 22 7737

HSV2-UL19-3355 + UGCACACGCACGCCACGGACAGG 23 7738

HSV2-UL19-3356 + GUGCA CACGCACGCCACGGACAGG 24 7739

HSV2-UL19-3357 + AUGGCCUCGGUGGCCAGG 18 7740

HSV2-UL19-3358 + AAUGGCCUCGGUGGCCAGG 19 7741

HSV2-UL19-260 + CAAUGGCCUCGGUGGCCAGG 20 2282

HSV2-UL19-3359 + GCAAUGGCCUCGGUGGCCAGG 21 7742 HSV2-UL19-3360 + GGCAAUGGCCUCGGUGGCCAGG 22 7743

HSV2-UL19-3361 + GGGCAAUGGCCUCGGUGGCCAGG 23 7744

HSV2-UL19-3362 + AGGGCAAUGGCCUCGGUGGCCAGG 24 7745

HSV2-UL19-3363 + ACCUCGAUCGUACUCAGG 18 7746

HSV2-UL19-3364 + CACCUCGAUCGUACUCAGG 19 7747

HSV2-UL19-289 + CCACCUCGAUCGUACUCAGG 20 2305

HSV2-UL19-3365 + GCCACCUCGAUCGUACUCAGG 21 7748

HSV2-UL19-3366 + CGCCACCUCGAUCGUACUCAGG 22 7749

HSV2-UL19-3367 + ACGCCACCUCGAUCGUACUCAGG 23 7750

HSV2-UL19-3368 + GACGCCACCUCGAUCGUACUCAGG 24 7751

HSV2-UL19-3369 + AAAUCAAAGAGUCUGCGG 18 7752

HSV2-UL19-3370 + AAAAU CAAAG AG UCU G CGG 19 7753

HSV2-UL19-80 + AAAAAUCAAAGAGUCUGCGG 20 498

HSV2-UL19-3371 + AAAAAAUCAAAGAGUCUGCGG 21 7754

HSV2-UL19-3372 + GAAAAAAUCAAAGAGUCUGCGG 22 7755

HSV2-UL19-3373 + CG AAA AAAU CAAAG AGUCUGCGG 23 7756

HSV2-UL19-3374 + G CG AAAAAAU CAAAG AG UCU GCG G 24 7757

HSV2-UL19-3375 + AACCCGGGGGGUCGCGGG 18 7758

HSV2-UL19-3376 + UAACCCGGGGGGUCGCGGG 19 7759

HSV2-UL19-298 + GU AACCCGGGGGGUCGCGGG 20 2310

HSV2-UL19-3377 + GGU AACCCGGGGGGUCGCGGG 21 7760

HSV2-UL19-3378 + CGG UAACCCGGGGGGUCGCGGG 22 7761

HSV2-UL19-3379 + CCGGU AACCCGGGGGGUCGCGGG 23 7762

HSV2-UL19-3380 + ACCGG UAACCCGGGGGGUCGCGGG 24 7763

HSV2-UL19-3381 + GGGCCGUCGCGGGCGAUG 18 7764

HSV2-UL19-3382 + CGGGCCGUCGCGGGCGAUG 19 7765

HSV2-UL19-60 + GCGGGCCGUCGCGGGCGAUG 20 483

HSV2-UL19-3383 + UGCGGGCCGUCGCGGGCGAUG 21 7766

HSV2-UL19-3384 + GUGCGGGCCGUCGCGGGCGAUG 22 7767

HSV2-UL19-3385 + GG UGCGGGCCGUCGCGGGCGAUG 23 7768

HSV2-UL19-3386 + GGGUGCGGGCCGUCGCGGGCGAUG 24 7769

HSV2-UL19-3387 + GCGGGCGAUGAGGGGCUG 18 7770

HSV2-UL19-3388 + CGCGGGCGAUGAGGGGCUG 19 7771

HSV2-UL19-270 + UCGCGGGCGAUGAGGGGCUG 20 2290

HSV2-UL19-3389 + GUCGCGGGCGAUGAGGGGCUG 21 7772

HSV2-UL19-3390 + CGUCGCGGGCGAUGAGGGGCUG 22 7773

HSV2-UL19-3391 + CCGUCGCGGGCGAUGAGGGGCUG 23 7774

HSV2-UL19-3392 + GCCGUCGCGGGCGAUGAGGGGCUG 24 7775

HSV2-UL19-3393 + CGGGCCGUCGCGGGCGAU 18 7776

HSV2-UL19-3394 + GCGGGCCGUCGCGGGCGAU 19 7777

HSV2-UL19-268 + UGCGGGCCGUCGCGGGCGAU 20 2289

HSV2-UL19-3395 + GUGCGGGCCGUCGCGGGCGAU 21 7778

HSV2-UL19-3396 + GGUGCGGGCCGUCGCGGGCGAU 22 7779

HSV2-UL19-3397 + GGGUGCGGGCCGUCGCGGGCGAU 23 7780

HSV2-UL19-3398 + GGGGUGCGGGCCGUCGCGGGCGAU 24 7781

HSV2-UL19-3399 + AGCGGCCAU UGGGUUCCU 18 7782 HSV2-UL19-3400 + GAGCGGCCAUUGGGUUCCU 19 7783

HSV2-UL19-3094 + GGAGCGGCCAUUGGGUUCCU 20 7477

HSV2-UL19-3401 + AGGAGCGGCCAUUGGGUUCCU 21 7784

HSV2-UL19-3402 + CAGGAGCGGCCAUUGGGUUCCU 22 7785

HSV2-UL19-3403 + GCAGGAGCGGCCAUUGGGUUCCU 23 7786

HSV2-UL19-3404 + GGCAGGAGCGGCCAUUGGGUUCCU 24 7787

HSV2-UL19-3405 + CGUUCAUGUAAGCCAGCU 18 7788

HSV2-UL19-3406 + UCGU UCAUGUAAGCCAGCU 19 7789

HSV2-UL19-272 + U U CG U U CAU G U AAG CCAGCU 20 2292

HSV2-UL19-3407 + CUUCGUUCAUGUAAGCCAGCU 21 7790

HSV2-UL19-3408 + CCUUCGUUCAUGUAAGCCAGCU 22 7791

HSV2-UL19-3409 + CCCUUCGUUCAUGUAAGCCAGCU 23 7792

HSV2-UL19-3410 + GCCCUUCGUUCAUGUAAGCCAGCU 24 7793

HSV2-UL19-3411 + CAUACAGGCUGUUUUCGU 18 7794

HSV2-UL19-3412 + UCAUACAGGCUGUUUUCGU 19 7795

HSV2-UL19-76 + GUCAUACAGGCUGUUUUCGU 20 494

HSV2-UL19-3413 + CGUCAUACAGGCUGUUUUCGU 21 7796

HSV2-UL19-3414 + ACGUCAUACAGGCUGUUUUCGU 22 7797

HSV2-UL19-3415 + UACGUCAUACAGGCUGUUUUCGU 23 7798

HSV2-UL19-3416 + CUACGUCAUACAGGCUGUUUUCGU 24 7799

HSV2-UL19-3417 + CGGGCUGCUCGACGGGGU 18 7800

HSV2-UL19-3418 + ACGGGCUGCUCGACGGGGU 19 7801

HSV2-UL19-265 + CACGGGCUGCUCGACGGGGU 20 2286

HSV2-UL19-3419 + GCACGGGCUGCUCGACGGGGU 21 7802

HSV2-UL19-3420 + UGCACGGGCUGCUCGACGGGGU 22 7803

HSV2-UL19-3421 + AUGCACGGGCUGCUCGACGGGGU 23 7804

HSV2-UL19-3422 + UAUGCACGGGCUGCUCGACGGGGU 24 7805

HSV2-UL19-3423 + GCGGCCAUUGGGUUCCUU 18 7806

HSV2-UL19-3424 + AGCGGCCAUUGGGUUCCUU 19 7807

HSV2-UL19-3095 + GAGCGGCCAU UGGGUUCCUU 20 7478

HSV2-UL19-3425 + GGAGCGGCCAUUGGGUUCCUU 21 7808

HSV2-UL19-3426 + AGGAGCGGCCAUUGGGUUCCUU 22 7809

HSV2-UL19-3427 + CAGGAGCGGCCAUUGGGUUCCUU 23 7810

HSV2-UL19-3428 + GCAGGAGCGGCCAUUGGGUUCCUU 24 7811

HSV2-UL19-3429 + CAGGCUGAAGGCGGCGUU 18 7812

HSV2-UL19-3430 + CCAGGCUGAAGGCGGCGUU 19 7813

HSV2-UL19-261 + GCCAGGCUGAAGGCGGCGUU 20 2283

HSV2-UL19-3431 + GGCCAGGCUGAAGGCGGCGUU 21 7814

HSV2-UL19-3432 + UGGCCAGGCUGAAGGCGGCGUU 22 7815

HSV2-UL19-3433 + GUGGCCAGGCUGAAGGCGGCGUU 23 7816

HSV2-UL19-3434 + GGUGGCCAGGCUGAAGGCGGCGUU 24 7817

HSV2-UL19-3435 - GAGCCUGCGAAUCCCCCA 18 7818

HSV2-UL19-3436 - GGAGCCUGCGAAUCCCCCA 19 7819

HSV2-UL19-3100 - UGGAGCCUGCGAAUCCCCCA 20 7483

HSV2-UL19-3437 - CCGCCUUCAGCCUGGCCA 18 7820

HSV2-UL19-3438 - GCCGCCUUCAGCCUGGCCA 19 7821 HSV2-UL19-218 - CGCCGCCUUCAGCCUGGCCA 20 2253

HSV2-UL19-3439 - ACGCCGCCUUCAGCCUGGCCA 21 7822

HSV2-UL19-3440 - AACGCCGCCUUCAGCCUGGCCA 22 7823

HSV2-UL19-3441 - GAACGCCGCCUUCAGCCUGGCCA 23 7824

HSV2-UL19-3442 - UGAACGCCGCCUUCAGCCUGGCCA 24 7825

HSV2-UL19-3443 - AGGCCAUUGCCCUGCUCA 18 7826

HSV2-UL19-3444 - GAGGCCAU UGCCCUGCUCA 19 7827

HSV2-UL19-25 - CGAGGCCAUUGCCCUGCUCA 20 453

HSV2-UL19-3445 - CCGAGGCCAUUGCCCUGCUCA 21 7828

HSV2-UL19-3446 - ACCGAGGCCAUUGCCCUGCUCA 22 7829

HSV2-UL19-3447 - CACCGAGGCCAUUGCCCUGCUCA 23 7830

HSV2-UL19-3448 - CCACCGAGGCCAUUGCCCUGCUCA 24 7831

HSV2-UL19-3449 - GCUCCAUCCUGAGUACGA 18 7832

HSV2-UL19-3450 - GGCUCCAUCCUGAGUACGA 19 7833

HSV2-UL19-201 - CGGCUCCAUCCUGAGUACGA 20 2239

HSV2-UL19-3451 - CCGGCUCCAUCCUGAGUACGA 21 7834

HSV2-UL19-3452 - ACCGGCUCCAUCCUGAGUACGA 22 7835

HSV2-UL19-3453 - CACCGGCUCCAUCCUGAGUACGA 23 7836

HSV2-UL19-3454 - CCACCGGCUCCAUCCUGAGUACGA 24 7837

HSV2-UL19-3455 - CACGGGGGAGGCCCUGGA 18 7838

HSV2-UL19-3456 - UCACGGGGGAGGCCCUGGA 19 7839

HSV2-UL19-31 - CUCACGGGGGAGGCCCUGGA 20 459

HSV2-UL19-3457 - GCUCACGGGGGAGGCCCUGGA 21 7840

HSV2-UL19-3458 - UGCUCACGGGGGAGGCCCUGGA 22 7841

HSV2-UL19-3459 - CUGCUCACGGGGGAGGCCCUGGA 23 7842

HSV2-UL19-3460 - CCUGCUCACGGGGGAGGCCCUGGA 24 7843

HSV2-UL19-3461 - CGGAGCUGGCUUACAUGA 18 7844

HSV2-UL19-3462 - CCGGAGCUGGCUUACAUGA 19 7845

HSV2-UL19-211 - CCCGGAGCUGGCUUACAUGA 20 2246

HSV2-UL19-3463 - UCCCGGAGCUGGCUUACAUGA 21 7846

HSV2-UL19-3464 - UUCCCGGAGCUGGCUUACAUGA 22 7847

HSV2-UL19-3465 - GUUCCCGGAGCUGGCUUACAUGA 23 7848

HSV2-UL19-3466 - AGU UCCCGGAGCUGGCUUACAUGA 24 7849

HSV2-UL19-3467 - GGCCAUUGCCCUGCUCAC 18 7850

HSV2-UL19-3468 - AGGCCAU UGCCCUGCUCAC 19 7851

HSV2-UL19-26 - GAGGCCAUUGCCCUGCUCAC 20 454

HSV2-UL19-3469 - CGAGGCCAUUGCCCUGCUCAC 21 7852

HSV2-UL19-3470 - CCGAGGCCAUUGCCCUGCUCAC 22 7853

HSV2-UL19-3471 - ACCGAGGCCAU UGCCCUGCUCAC 23 7854

HSV2-UL19-3472 - CACCGAGGCCAUUGCCCUGCUCAC 24 7855

HSV2-UL19-3473 - UUCCCGGAGCUGGCUUAC 18 7856

HSV2-UL19-3474 - GUUCCCGGAGCUGGCUUAC 19 7857

HSV2-UL19-210 - AGUUCCCGGAGCUGGCUUAC 20 2245

HSV2-UL19-3475 - AAGUUCCCGGAGCUGGCUUAC 21 7858

HSV2-UL19-3476 - CAAGUUCCCGGAGCUGGCUUAC 22 7859

HSV2-UL19-3477 - CCAAGUUCCCGGAGCUGGCUUAC 23 7860 HSV2-UL19-3478 - ACCAAGUUCCCGGAGCUGGCUUAC 24 7861

HSV2-UL19-3479 - GGAGCCUGCGAAUCCCCC 18 7862

HSV2-UL19-3480 - UGGAGCCUGCGAAUCCCCC 19 7863

HSV2-UL19-3481 - AUGGAGCCUGCGAAUCCCCC 20 7864

HSV2-UL19-3482 - CUGCUCACGGGGGAGGCC 18 7865

HSV2-UL19-3483 - CCUGCUCACGGGGGAGGCC 19 7866

HSV2-UL19-224 - CCCUGCUCACGGGGGAGGCC 20 2255

HSV2-UL19-3484 - GCCCUGCUCACGGGGGAGGCC 21 7867

HSV2-UL19-3485 - UGCCCUGCUCACGGGGGAGGCC 22 7868

HSV2-UL19-3486 - UUGCCCUGCUCACGGGGGAGGCC 23 7869

HSV2-UL19-3487 - AUUGCCCUGCUCACGGGGGAGGCC 24 7870

HSV2-UL19-3488 - GUCAUCGACCGCCGGGCC 18 7871

HSV2-UL19-3489 - GGUCAUCGACCGCCGGGCC 19 7872

HSV2-UL19-217 - AGGUCAUCGACCGCCGGGCC 20 2252

HSV2-UL19-3490 - AAGGUCAUCGACCGCCGGGCC 21 7873

HSV2-UL19-3491 - GAAGGUCAUCGACCGCCGGGCC 22 7874

HSV2-UL19-3492 - CGAAGGUCAUCGACCGCCGGGCC 23 7875

HSV2-UL19-3493 - ACGAAGGUCAUCGACCGCCGGGCC 24 7876

HSV2-UL19-3494 - G CAACG UCCAGGCCGUCC 18 7877

HSV2-UL19-3495 - CGCAACGUCCAGGCCGUCC 19 7878

HSV2-UL19-3092 - G CG CAACG UCCAGGCCGUCC 20 7475

HSV2-UL19-3496 - CGCGCAACGUCCAGGCCGUCC 21 7879

HSV2-UL19-3497 - GCGCGCAACGUCCAGGCCGUCC 22 7880

HSV2-UL19-3498 - CGCGCGCAACGUCCAGGCCGUCC 23 7881

HSV2-UL19-3499 - UCGCGCGCAACGUCCAGGCCGUCC 24 7882

HSV2-UL19-3500 - GCGUGUGCACCAAGUUCC 18 7883

HSV2-UL19-3501 - UGCGUGUGCACCAAGUUCC 19 7884

HSV2-UL19-14 - GUGCGUGUGCACCAAGUUCC 20 393

HSV2-UL19-3502 - CGUGCGUGUG CACCAAG U U CC 21 7885

HSV2-UL19-3503 - GCGUGCGUGUGCACCAAGUUCC 22 7886

HSV2-UL19-3504 - GGCGUGCGUGUGCACCAAGUUCC 23 7887

HSV2-UL19-3505 - UGGCGUGCGUGUGCACCAAGUUCC 24 7888

HSV2-UL19-3506 - GAGGCCAUUGCCCUGCUC 18 7889

HSV2-UL19-3507 - CGAGGCCAUUGCCCUGCUC 19 7890

HSV2-UL19-219 - CCGAGGCCAUUGCCCUGCUC 20 2254

HSV2-UL19-3508 - ACCGAGGCCAU UGCCCUGCUC 21 7891

HSV2-UL19-3509 - CACCGAGGCCAUUGCCCUGCUC 22 7892

HSV2-UL19-3510 - CCACCGAGGCCAUUGCCCUGCUC 23 7893

HSV2-UL19-3511 - GCCACCGAGGCCAUUGCCCUGCUC 24 7894

HSV2-UL19-3512 - CGCAACGUCCAGGCCGUC 18 7895

HSV2-UL19-3513 - GCGCAACGUCCAGGCCGUC 19 7896

HSV2-UL19-3514 - CGCGCAACGUCCAGGCCGUC 20 7897

HSV2-UL19-3515 - GCGCGCAACGUCCAGGCCGUC 21 7898

HSV2-UL19-3516 - CGCGCGCAACGUCCAGGCCGUC 22 7899

HSV2-UL19-3517 - UCGCGCGCAACGUCCAGGCCGUC 23 7900

HSV2-UL19-3518 - CUCGCGCGCAACGUCCAGGCCGUC 24 7901 HSV2-UL19-3519 - UGCGUGUGCACCAAGUUC 18 7902

HSV2-UL19-3520 - GUGCGUGUGCACCAAGUUC 19 7903

HSV2-UL19-208 - CGUGCGUGUGCACCAAGUUC 20 2244

HSV2-UL19-3521 - GCGUGCGUGUGCACCAAGUUC 21 7904

HSV2-UL19-3522 - GGCGUGCGUGUGCACCAAGUUC 22 7905

HSV2-UL19-3523 - UGGCGUGCGUGUGCACCAAGUUC 23 7906

HSV2-UL19-3524 - GUGGCGUGCGUGUGCACCAAGUUC 24 7907

HSV2-UL19-3525 - UGUCGCUCGUGCGCUUUC 18 7908

HSV2-UL19-3526 - CUGUCGCUCGUGCGCUUUC 19 7909

HSV2-UL19-11 - CCUGUCGCUCGUGCGCUUUC 20 391

HSV2-UL19-3527 - CCCUGUCGCUCGUGCGCUUUC 21 7910

HSV2-UL19-3528 - ACCCUGUCGCUCGUGCGCUUUC 22 7911

HSV2-UL19-3529 - CACCCUGUCGCUCGUGCGCUUUC 23 7912

HSV2-UL19-3530 - ACACCCUGUCGCUCGUGCGCUUUC 24 7913

HSV2-UL19-3531 - AGCUGGCUUACAUGAACG 18 7914

HSV2-UL19-3532 - GAGCUGGCUUACAUGAACG 19 7915

HSV2-UL19-212 - GG AGCUGGCUUACAUGAACG 20 2247

HSV2-UL19-3533 - CGGAGCUGGCU UACAUGAACG 21 7916

HSV2-UL19-3534 - CCGG AGCUGGCUUACAUGAACG 22 7917

HSV2-UL19-3535 - CCCGGAGCUGGCUUACAUGAACG 23 7918

HSV2-UL19-3536 - UCCCGGAGCUGGCU UACAUGAACG 24 7919

HSV2-UL19-3537 - GCCAUUGCCCUGCUCACG 18 7920

HSV2-UL19-3538 - GGCCAUUGCCCUGCUCACG 19 7921

HSV2-UL19-27 - AGGCCAUUGCCCUGCUCACG 20 455

HSV2-UL19-3539 - GAGGCCAU UGCCCUGCUCACG 21 7922

HSV2-UL19-3540 - CGAGGCCAUUGCCCUGCUCACG 22 7923

HSV2-UL19-3541 - CCGAGGCCAUUGCCCUGCUCACG 23 7924

HSV2-UL19-3542 - ACCGAGGCCAUUGCCCUGCUCACG 24 7925

HSV2-UL19-3543 - GACGAAGGUCAUCGACCG 18 7926

HSV2-UL19-3544 - UGACGAAGGUCAUCGACCG 19 7927

HSV2-UL19-216 - AUGACGAAGGUCAUCGACCG 20 2251

HSV2-UL19-3545 - CAUGACGAAGGUCAUCGACCG 21 7928

HSV2-UL19-3546 - ACAU GACGAAGGUCAUCGACCG 22 7929

HSV2-UL19-3547 - UACAUGACGAAGGUCAUCGACCG 23 7930

HSV2-UL19-3548 - UUACAUGACGAAGGUCAUCGACCG 24 7931

HSV2-UL19-3549 - GCGACGGCCCGCACCCCG 18 7932

HSV2-UL19-3550 - CGCGACGGCCCGCACCCCG 19 7933

HSV2-UL19-214 - CCGCGACGGCCCGCACCCCG 20 2249

HSV2-UL19-3551 - CCCGCGACGGCCCGCACCCCG 21 7934

HSV2-UL19-3552 - GCCCGCGACGGCCCGCACCCCG 22 7935

HSV2-UL19-3553 - CGCCCGCGACGGCCCGCACCCCG 23 7936

HSV2-UL19-3554 - UCGCCCGCGACGGCCCGCACCCCG 24 7937

HSV2-UL19-3555 - UCGCCGCCGUGCGCUCCG 18 7938

HSV2-UL19-3556 - UUCGCCGCCGUGCGCUCCG 19 7939

HSV2-UL19-202 - UUUCGCCGCCGUGCGCUCCG 20 2240

HSV2-UL19-3557 - UU UUCGCCGCCGUGCGCUCCG 21 7940 HSV2-UL19-3558 - UUUUUCGCCGCCGUGCGCUCCG 22 7941

HSV2-UL19-3559 - UUUUUUCGCCGCCGUGCGCUCCG 23 7942

HSV2-UL19-3560 - AUU UUUUCGCCGCCGUGCGCUCCG 24 7943

HSV2-UL19-3561 - CCAUUGCCCUGCUCACGG 18 7944

HSV2-UL19-3562 - GCCAUUGCCCUGCUCACGG 19 7945

HSV2-UL19-28 - GGCCAUUGCCCUGCUCACGG 20 456

HSV2-UL19-3563 - AGGCCAUUGCCCUGCUCACGG 21 7946

HSV2-UL19-3564 - GAGGCCAUUGCCCUGCUCACGG 22 7947

HSV2-UL19-3565 - CGAGGCCAUUGCCCUGCUCACGG 23 7948

HSV2-UL19-3566 - CCGAGGCCAUUGCCCUGCUCACGG 24 7949

HSV2-UL19-3567 - GGGGAGGCCCUGGACGGG 18 7950

HSV2-UL19-3568 - GGGGGAGGCCCUGGACGGG 19 7951

HSV2-UL19-227 - CGGGGGAGGCCCUGGACGGG 20 2257

HSV2-UL19-3569 - ACGGGGGAGGCCCUGGACGGG 21 7952

HSV2-UL19-3570 - CACGGGGGAGGCCCUGGACGGG 22 7953

HSV2-UL19-3571 - UCACGGGGGAGGCCCUGGACGGG 23 7954

HSV2-UL19-3572 - CUCACGGGGGAGGCCCUGGACGGG 24 7955

HSV2-UL19-3573 - UCACGGGGGAGGCCCUGG 18 7956

HSV2-UL19-3574 - CUCACGGGGGAGGCCCUGG 19 7957

HSV2-UL19-225 - GCUCACGGGGGAGGCCCUGG 20 2256

HSV2-UL19-3575 - UGCUCACGGGGGAGGCCCUGG 21 7958

HSV2-UL19-3576 - CUGCUCACGGGGGAGGCCCUGG 22 7959

HSV2-UL19-3577 - CCUGCUCACGGGGGAGGCCCUGG 23 7960

HSV2-UL19-3578 - CCCUGCUCACGGGGGAGGCCCUGG 24 7961

HSV2-UL19-3579 - CCCGUGCAUAAUUACAUG 18 7962

HSV2-UL19-3580 - GCCCGUGCAUAAUUACAUG 19 7963

HSV2-UL19-215 - AGCCCGUGCAUAAUUACAUG 20 2250

HSV2-UL19-3581 - CAGCCCGUGCAUAAUUACAUG 21 7964

HSV2-UL19-3582 - GCAGCCCGUGCAUAAUUACAUG 22 7965

HSV2-UL19-3583 - AGCAG CCCG UGCAUAAU U ACAU G 23 7966

HSV2-UL19-3584 - GAGCAGCCCGUGCAUAAUUACAUG 24 7967

HSV2-UL19-3585 - GUAGAGUUUGACGCCCUG 18 7968

HSV2-UL19-3586 - CGUAGAGUUUGACGCCCUG 19 7969

HSV2-UL19-204 - ACGUAGAGUUUGACGCCCUG 20 2242

HSV2-UL19-3587 - GACGUAGAGUUUGACGCCCUG 21 7970

HSV2-UL19-3588 - UGACGUAGAGUUUGACGCCCUG 22 7971

HSV2-UL19-3589 - AUGACGUAGAGUUUGACGCCCUG 23 7972

HSV2-UL19-3590 - UAUGACGUAGAGUUUGACGCCCUG 24 7973

HSV2-UL19-3591 - CAACG U CCAG G CCG U CCU 18 7974

HSV2-UL19-3592 - GCAACGUCCAGGCCGUCCU 19 7975

HSV2-UL19-3085 - CGCAACGUCCAGGCCGUCCU 20 7468

HSV2-UL19-3593 - GCGCAACGUCCAGGCCGUCCU 21 7976

HSV2-UL19-3594 - CGCGCAACGUCCAGGCCGUCCU 22 7977

HSV2-UL19-3595 - GCGCGCAACGUCCAGGCCGUCCU 23 7978

HSV2-UL19-3596 - CGCGCGCAACGUCCAGGCCGUCCU 24 7979

HSV2-UL19-3597 - ACGAAGGGCGUGUGCAGU 18 7980 HSV2-UL19-3598 - AACGAAGGGCGUGUGCAGU 19 7981

HSV2-UL19-213 - GAACGAAGGGCGUGUGCAGU 20 2248

HSV2-UL19-3599 - UGAACGAAGGGCGUGUGCAGU 21 7982

HSV2-UL19-3600 - AUG AACGAAGGGCGUGUGCAGU 22 7983

HSV2-UL19-3601 - CAUG AACGAAGGGCGUGUGCAGU 23 7984

HSV2-UL19-3602 - ACAU G AACG AAG GGCGUGUGCAGU 24 7985

HSV2-UL19-3603 - CUGUCGCUCGUGCGCUUU 18 7986

HSV2-UL19-3604 - CCUGUCGCUCGUGCGCUUU 19 7987

HSV2-UL19-206 - CCCUGUCGCUCGUGCGCUUU 20 2243

HSV2-UL19-3605 - ACCCUGUCGCUCGUGCGCUUU 21 7988

HSV2-UL19-3606 - CACCCUGUCGCUCGUGCGCUUU 22 7989

HSV2-UL19-3607 - ACACCCUGUCGCUCGUGCGCUUU 23 7990

HSV2-UL19-3608 - AACACCCU G U CG CU CG UG CG CU U U 24 7991

Table 6E provides exemplary targeting domains for knocking out the UL19 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene), and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 6E

HSV2-UL19-3623 + GGCCACCAGAUUGGCGGGAUGCA 23 8006

HSV2-UL19-3624 + GUUCCAGUAGCUGGUGAUGCA 21 8007

HSV2-UL19-3625 + GU UGUUCCAGUAGCUGGUGAUGCA 24

8008

HSV2-UL19-3626 + GGUGGGCGGGGUCGGUCA 18 8009

HSV2-UL19-3627 + GGGUGGGCGGGGUCGGUCA 19 8010

HSV2-UL19-3628 + GCGGGUGGGCGGGGUCGGUCA 21 8011

HSV2-UL19-3629 + GCAGCGGGUGGGCGGGGUCGGUCA 24

8012

HSV2-UL19-1436 + GUACGGAUUCGCGGCCUCGA 20 1676

HSV2-UL19-3630 + GGUCGCUGGCGCAGGUGA 18 8013

HSV2-UL19-3631 + GGGUCGCUGGCGCAGGUGA 19 8014

HSV2-UL19-2516 + GGGGUCGCUGGCGCAGGUGA 20 2680

HSV2-UL19-3632 + GCGGGG UCGCUGGCGCAGGUGA 22 8015

HSV2-UL19-3633 + GGCGGGGUCGCUGGCGCAGGUGA 23 8016

HSV2-UL19-3634 + GGGCGGGGUCGCUGGCGCAGG UGA 24

8017

HSV2-UL19-3635 + GU UGAUGUCCGUGGCCAC 18 8018

HSV2-UL19-3636 + GUAGUUGAUGUCCGUGGCCAC 21 8019

HSV2-UL19-3637 + GCUCCGCU UCAGCUCGGCGAC 21 8020

HSV2-UL19-3638 + GCACGACGGUGAACCCGAACC 21 8021

HSV2-UL19-3639 + GCCGCACGACGGUGAACCCGAACC 24 8022

HSV2-UL19-2541 + GCCGCCGGCCGCGCGUCCCC 20 2704

HSV2-UL19-3640 + GAGAACCGCUGCGACGCCC 19 8023

HSV2-UL19-3641 + GUACGAGAACCGCUGCGACGCCC 23 8024

HSV2-UL19-3642 + GUCGCUGGCAGCGGUGGCC 19 8025

HSV2-UL19-3643 + GCACGUCGCUGGCAGCGGUGGCC 23 8026

HSV2-UL19-3644 + GAACACGUGCUCGUUGCC 18 8027

HSV2-UL19-3645 + GCAGAACACGUGCUCGU UGCC 21 8028

HSV2-UL19-3646 + GCGCAGAACACGUGCUCGUUGCC 23 8029

HSV2-UL19-3647 + GGCGCAGAACACGUGCUCGU UGCC 24 8030

HSV2-UL19-3648 + GCU UCCUGAAACAGGCCGC 19 8031

HSV2-UL19-2518 + GGCUUCCUGAAACAGGCCGC 20 2682

HSV2-UL19-3649 + GUAGGCUUCCUGAAACAGGCCGC 23 8032

HSV2-UL19-3650 + GGUAGGCUUCCUGAAACAGGCCGC 24 8033

HSV2-UL19-3651 + GGCGUCGGCCGCGCGGGC 18 8034

HSV2-UL19-2655 + GCGGCGUCGGCCGCGCGGGC 20 2774

HSV2-UL19-3652 + GGCGGCGUCGGCCGCGCGGGC 21 8035

HSV2-UL19-3653 + GUCGGCGGCGUCGGCCGCGCGGGC 24 8036

HSV2-UL19-3654 + GACCACG CG CCAG G U GG C 18 8037

HSV2-UL19-3655 + GUUGACCACGCGCCAGGUGGC 21 8038

HSV2-UL19-3656 + GCCGUUGACCACGCGCCAGGUGGC 24 8039

HSV2-UL19-1411 + G CCCGCG A AG AAG U CG AACG 20 1651

HSV2-UL19-3657 + G CAG CAG G U AA AACACCG 18 8040

HSV2-UL19-3658 + GCU UGCAGCAGGUAAAACACCG 22 8041

HSV2-UL19-3659 + G G C U U G CAG CAG G U AA AACACCG 23 8042 HSV2-UL19-3660 + GCGUCGAUGU UCAUGAGCG 19 8043

HSV2-UL19-3661 + GCCGCGUCGAUGUUCAUGAGCG 22 8044

HSV2-UL19-3662 + GGCCGCG UCGAUGUUCAUGAGCG 23 8045

HSV2-UL19-3663 + GUGGCCGCGAAGGGCCGCG 19 8046

HSV2-UL19-3664 + GCCGUGGCCGCGAAGGGCCGCG 22 8047

HSV2-UL19-3665 + GGCCGUGGCCGCGAAGGGCCGCG 23 8048

HSV2-UL19-3666 + GCCCAGCUCCGGGCCCGG 18 8049

HSV2-UL19-3667 + GCCCGCCCAGCUCCGGGCCCGG 22 8050

HSV2-UL19-3668 + GUCCACGUUUCCGCGCGG 18 8051

HSV2-UL19-3669 + GGUCCACGU UUCCGCGCGG 19 8052

HSV2-UL19-3670 + GGGCGCUGCGUAGCAGGG 18 8053

HSV2-UL19-1113 + GCGGGCGCUGCG UAGCAGGG 20 1353

HSV2-UL19-3671 + GCGGGAUGCAGCGGGUGGG 19 8054

HSV2-UL19-1300 + GGCGGGAUGCAGCGGGUGGG 20 1540

HSV2-UL19-3672 + GAU UGGCGGGAUGCAGCGGGUGGG 24

8055

HSV2-UL19-3673 + GCGGCGAAGCGCUCCAUGG 19 8056

HSV2-UL19-3674 + GCGCGGCGAAGCGCUCCAUGG 21 8057

HSV2-UL19-3675 + GUGCGCGGCGAAGCGCUCCAUGG 23 8058

HSV2-UL19-3676 + GUGUUGGCCACCAGAUUGG 19 8059

HSV2-UL19-3677 + GACCGUGUUGGCCACCAGAUUGG 23 8060

HSV2-UL19-3118 + GACGUUGCGCGCGAGCUG 18 7501

HSV2-UL19-3119 + GGACGUUGCGCGCGAGCUG 19 7502

HSV2-UL19-3120 + GCCUGGACGUUGCGCGCGAGCUG 23 7503

HSV2-UL19-3121 + GGCCUGGACGUUGCGCGCGAGCUG 24 7504

HSV2-UL19-3678 + GUGCUGCACCACGGGCUG 18 8061

HSV2-UL19-2593 + GCGUGCUGCACCACGGGCUG 20 2737

HSV2-UL19-3679 + GGCGUGCUGCACCACGGGCUG 21 8062

HSV2-UL19-3680 + GGGCGUGCUGCACCACGGGCUG 22 8063

HSV2-UL19-3681 + GCGGGCGUGCUGCACCACGGGCUG 24 8064

HSV2-UL19-3682 + GAGGGAACGCGCGGGGCU 18 8065

HSV2-UL19-3683 + GGAGGGAACGCGCGGGGCU 19 8066

HSV2-UL19-2733 + GGGAGGGAACGCGCGGGGCU 20 2831

HSV2-UL19-3684 + GGGGAGGGAACGCGCGGGGCU 21 8067

HSV2-UL19-3685 + GCCGGGGAGGGAACGCGCGGGGCU 24 8068

HSV2-UL19-3686 + GCCGCGACGUACGCCCCGU 19 8069

HSV2-UL19-2716 + GGCCGCGACGUACGCCCCGU 20 2821

HSV2-UL19-3687 + GGGCCGCGACGUACGCCCCGU 21 8070

HSV2-UL19-3688 + GGGGCCGCGACGUACGCCCCGU 22 8071

HSV2-UL19-3689 + G U AG AAG U AU U CGCCAU U 18 8072

HSV2-UL19-3690 + GACGUAGAAGUAUUCGCCAU U 21 8073

HSV2-UL19-3691 + GGACGUAGAAGUAUUCGCCAUU 22 8074

HSV2-UL19-3692 + GUUGUCUGGGGGGCGCU U 18 8075

HSV2-UL19-2756 + GCGU UGUCUGGGGGGCGCUU 20 2845

HSV2-UL19-3693 + GCGCGU UGUCUGGGGGGCGCUU 22 8076

HSV2-UL19-3694 - GAGCGUCUCAUCGUGGAAA 19 8077 HSV2-UL19-706 - GGAGCGUCUCAUCGUGGAAA 20 946

HSV2-UL19-3695 - GCCUGGAGCGUCUCAUCGUGGAAA 24 8078

HSV2-UL19-3696 - GCGCGGGCGGCCGGUCGA 18 8079

HSV2-UL19-3697 - GACACGCGCGGGCGGCCGGUCGA 23 8080

HSV2-UL19-2002 - GACAACGGGCGCCUGGCGAC 20 2347

HSV2-UL19-3698 - GCCGCGCGGAAACGUGGAC 19 8081

HSV2-UL19-2173 - GCUCAAGACGGGCCUCCACC 20 2462

HSV2-UL19-3699 - GAACCACGACCCCGUCGAGGCC 22 8082

HSV2-UL19-3700 - GUGAACCACGACCCCGUCGAGGCC 24 8083

HSV2-UL19-3701 - GGGGCGCUGCUGCACCGC 18 8084

HSV2-UL19-3702 - GGGGGCGCUGCUGCACCGC 19 8085

HSV2-UL19-3703 - GCGGGGGCGCUGCUGCACCGC 21 8086

HSV2-UL19-3704 - GUGCAGUUUAAGCGCCCGC 19 8087

HSV2-UL19-3705 - G ACG UGCAGU U U AAGCGCCCGC 22 8088

HSV2-UL19-3706 - GCGACGUGCAGUUUAAGCGCCCGC 24 8089

HSV2-UL19-3707 - GCCCCCCAGACAACGCGCGUGCGC 24 8090

HSV2-UL19-3708 - GGGCAGGCUCAGGCCGAG 18 8091

HSV2-UL19-2123 - GCGGGCAGGCUCAGGCCGAG 20 2431

HSV2-UL19-3709 - GGCGGGCAGGCUCAGGCCGAG 21 8092

HSV2-UL19-3710 - GGGCGGGCAGGCUCAGGCCGAG 22 8093

HSV2-UL19-3711 - GAGGCGAACCGGGAGACG 18 8094

HSV2-UL19-3712 - GCCUCGCCCACGGCCGGGUCCG 22 8095

HSV2-UL19-3713 - GCGCCUCGCCCACGGCCGGGUCCG 24 8096

HSV2-UL19-3714 - GCCGGGUCCGGUGGGUCG 18 8097

HSV2-UL19-3715 - GGCCGGGUCCGGUGGGUCG 19 8098

HSV2-UL19-3716 - GCAGCGCCUCGCCCACGG 18 8099

HSV2-UL19-3717 - GGCAGCGCCUCGCCCACGG 19 8100

HSV2-UL19-3718 - GCGGCAGCGCCUCGCCCACGG 21 8101

HSV2-UL19-3719 - GGCGGCAGCGCCUCGCCCACGG 22 8102

HSV2-UL19-629 - GCCCGCCACGAAACGGGCGG 20 869

HSV2-UL19-3720 - GGCCCGCCACGAAACGGGCGG 21 8103

HSV2-UL19-3721 - GUGGCCCGCCACGAAACGGGCGG 23 8104

HSV2-UL19-3722 - GGUGGCCCGCCACGAAACGGGCGG 24 8105

HSV2-UL19-3723 - GCCGGCAUGGACCACGGG 18 8106

HSV2-UL19-2206 - GCGCCGGCAUGGACCACGGG 20 2485

HSV2-UL19-3724 - GCGCGCCGGCAUGGACCACGGG 22 8107

HSV2-UL19-3725 - GGCGCGCCGGCAUGGACCACGGG 23 8108

HSV2-UL19-606 - GCUGACCUACGCGCUCAUGG 20 846

HSV2-UL19-3726 - GCGCUGACCUACGCGCUCAUGG 22 8109

HSV2-UL19-3727 - GACCACACCAUCCAAAAUG 19 8110

HSV2-UL19-2156 - GGACCACACCAUCCAAAAUG 20 2452

HSV2-UL19-3728 - GGACCGCCACCGAGACUG 18 8111

HSV2-UL19-3729 - GCCCUGGACCGCCACCGAGACUG 23 8112

HSV2-UL19-3730 - GGCCCUGGACCGCCACCGAGACUG 24 8113

HSV2-UL19-3731 - GACG UGGGCCGCCACCUG 18 8114

HSV2-UL19-3732 - GACGACGUGGGCCGCCACCUG 21 8115 HSV2-UL19-3733 - GGACGACGUGGGCCGCCACCUG 22 8116

HSV2-UL19-3734 - GUCCCGGAGAUCGCCCCCGGUG 22 8117

HSV2-UL19-3735 - GGUCCCGGAGAUCGCCCCCGGUG 23 8118

HSV2-UL19-2174 - GACGGGCCUCCACCCCGGGU 20 2463

HSV2-UL19-3736 - GACCACGGGCAGGAUGCCGUGU 22 8119

HSV2-UL19-3737 - GGACCACGGGCAGGAUGCCGUGU 23 8120

Table 6F provides exemplary targeting domains for knocking out the UL19 gene selected according to the six tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 6F

HSV2-UL19-3756 + UUGUUCCAGUAGCUGGUGAUGCA 23 8139

HSV2-UL19-1304 + CGGGUGGGCGGGGUCGGUCA 20 1544

HSV2-UL19-3757 + AGCGGGUGGGCGGGGUCGGUCA 22 8140

HSV2-UL19-3758 + CAGCGGGUGGGCGGGGUCGGUCA 23 8141

HSV2-U L19-3759 + ACGGAUUCGCGGCCUCGA 18 8142

HSV2-U L19-3760 + UACGGAUUCGCGGCCUCGA 19 8143

HSV2-UL19-3761 + CGUACGGAU UCGCGGCCUCGA 21 8144

HSV2-U L19-3762 + CCGUACGGAUUCGCGGCCUCGA 22 8145

HSV2-U L19-3763 + CCCGUACGGAUUCGCGGCCUCGA 23 8146

HSV2-UL19-3764 + CCCCGUACGGAUUCGCGGCCUCGA 24 8147

HSV2-U L19-3765 + CGGGGUCGCUGGCGCAGGUGA 21 8148

HSV2-UL19-3766 + AGUUGAUG UCCGUGGCCAC 19 8149

HSV2-UL19-1166 + UAGUUGAUGUCCGUGGCCAC 20 1406

HSV2-U L19-3767 + AGUAGUUGAUGUCCGUGGCCAC 22 8150

HSV2-UL19-3768 + AAG UAGU UGAUGUCCGUGGCCAC 23 8151

HSV2-U L19-3769 + AAAGUAGUUGAUG UCCGUGGCCAC 24 8152

HSV2-U L19-3770 + CCGCUUCAGCUCGGCGAC 18 8153

HSV2-UL19-3771 + UCCGCUUCAGCUCGGCGAC 19 8154

HSV2-U L19-2771 + CUCCGCU UCAGCUCGGCG AC 20 2856

HSV2-UL19-3772 + AGCUCCGCUUCAGCUCGGCGAC 22 8155

HSV2-UL19-3773 + AAGCUCCGCUUCAGCUCGGCGAC 23 8156

HSV2-U L19-3774 + AAAGCUCCGCUUCAGCUCGGCGAC 24 8157

HSV2-UL19-3775 + CGACGGUGAACCCGAACC 18 8158

HSV2-UL19-3776 + ACGACGGUGAACCCGAACC 19 8159

HSV2-U L19-1222 + CACGACGGUGAACCCGAACC 20 1462

HSV2-UL19-3777 + CGCACG ACGG U G AACCCG AACC 22 8160

HSV2-U L19-3778 + CCGCACGACGGUGAACCCGAACC 23 8161

HSV2-U L19-3779 + CG CCG G CCGCG CG U CCCC 18 8162

HSV2-UL19-3780 + CCGCCGGCCGCGCGUCCCC 19 8163

HSV2-U L19-3781 + CGCCGCCGGCCGCGCGUCCCC 21 8164

HSV2-U L19-3782 + ACGCCGCCGGCCGCGCGUCCCC 22 8165

HSV2-UL19-3783 + CACGCCGCCGGCCGCGCGUCCCC 23 8166

HSV2-U L19-3784 + ACACGCCGCCGGCCGCGCGUCCCC 24 8167

HSV2-UL19-3785 + AGAACCGCUGCGACGCCC 18 8168

HSV2-UL19-2535 + CGAGAACCGCUGCGACGCCC 20 2698

HSV2-U L19-3786 + ACG AGAACCGCUGCGACGCCC 21 8169

HSV2-UL19-3787 + UACGAGAACCGCUGCGACGCCC 22 8170

HSV2-U L19-3788 + CGUACGAGAACCGCUGCGACGCCC 24 8171

HSV2-U L19-3789 + AGCGUCUCCCGGUUCGCC 18 8172

HSV2-UL19-3790 + CAGCGUCUCCCGGUUCGCC 19 8173

HSV2-UL19-2757 + CCAGCGUCUCCCGGUUCGCC 20 2846

HSV2-UL19-3791 + UCCAGCGUCUCCCGGUUCGCC 21 8174

HSV2-UL19-3792 + AUCCAGCGUCUCCCGGU UCGCC 22 8175

HSV2-U L19-3793 + CAUCCAGCGUCUCCCGG UUCGCC 23 8176

HSV2-UL19-3794 + UCAUCCAGCGUCUCCCGGU UCGCC 24 8177

HSV2-UL19-3795 + UCGCUGGCAGCGGUGGCC 18 8178 HSV2-UL19-2521 + CGUCGCUGGCAGCGGUGGCC 20 2685

HSV2-UL19-3796 + ACGUCGCUGGCAGCGGUGGCC 21 8179

HSV2-UL19-3797 + CACGUCGCUGGCAGCGG UGGCC 22 8180

HSV2-UL19-3798 + UGCACGUCGCUGGCAGCGGUGGCC 24 8181

HSV2-U L19-3799 + AGAACACGUGCUCGUUGCC 19 8182

HSV2-U L19-2680 + CAGAACACGUGCUCGU UGCC 20 2795

HSV2-UL19-3800 + CGCAGAACACGUGCUCGUUGCC 22 8183

HSV2-U L19-3801 + CUUCCUGAAACAGGCCGC 18 8184

HSV2-U L19-3802 + AGGCUUCCUGAAACAGGCCGC 21 8185

HSV2-UL19-3803 + UAGGCUUCCUGAAACAGGCCGC 22 8186

HSV2-U L19-3804 + CGGCGUCGGCCGCGCGGGC 19 8187

HSV2-UL19-3805 + CGGCGGCGUCGGCCGCGCGGGC 22 8188

HSV2-UL19-3806 + UCGGCGGCGUCGGCCGCGCGGGC 23 8189

HSV2-U L19-3807 + UGACCACGCGCCAGGUGGC 19 8190

HSV2-UL19-2690 + UUGACCACGCGCCAGGUGGC 20 2804

HSV2-U L19-3808 + CGU UGACCACGCGCCAGGUGGC 22 8191

HSV2-U L19-3809 + CCG U UGACCACGCGCCAGGUGGC 23 8192

HSV2-UL19-3810 + CCGCGAAGAAGUCGAACG 18 8193

HSV2-U L19-3811 + CCCGCGAAGAAGUCGAACG 19 8194

HSV2-UL19-3812 + CGCCCGCGAAGAAGUCGAACG 21 8195

HSV2-UL19-3813 + ACGCCCGCGAAGAAGUCGAACG 22 8196

HSV2-U L19-3814 + CACGCCCGCGAAGAAGUCGAACG 23 8197

HSV2-UL19-3815 + CCACGCCCGCG AAG AAG UCGAACG 24 8198

HSV2-UL19-3816 + U G C AG C AG G U A AAACACCG 19 8199

HSV2-U L19-2681 + U U G CAG CAG G U A AAACACCG 20 2796

HSV2-UL19-3817 + CUUGCAGCAGGUAAAACACCG 21 8200

HSV2-U L19-3818 + CG G CU U G CAG CAG G U A AAACACCG 24 8201

HSV2-U L19-3819 + CGAGGGGCGGCAGCAGCG 18 8202

HSV2-UL19-3820 + ACGAGGGGCGGCAGCAGCG 19 8203

HSV2-U L19-2661 + CACGAGGGGCGGCAGCAGCG 20 2779

HSV2-U L19-3821 + ACACGAGGGGCGGCAGCAGCG 21 8204

HSV2-UL19-3822 + CACACGAGGGGCGGCAGCAGCG 22 8205

HSV2-U L19-3823 + CCACACGAGGGGCGGCAGCAGCG 23 8206

HSV2-UL19-3824 + CCCACACGAGGGGCGGCAGCAGCG 24 8207

HSV2-UL19-3825 + CGUCGAUGU UCAUGAGCG 18 8208

HSV2-U L19-2721 + CGCGUCGAUGU UCAUGAGCG 20 2825

HSV2-UL19-3826 + CCGCGUCGAUGUUCAUGAGCG 21 8209

HSV2-U L19-3827 + CGGCCGCGUCGAUG UUCAUGAGCG 24 8210

HSV2-U L19-3828 + UGGCCGCGAAGGGCCGCG 18 8211

HSV2-UL19-2538 + CGUGGCCGCGAAGGGCCGCG 20 2701

HSV2-UL19-3829 + CCGUGGCCGCGAAGGGCCGCG 21 8212

HSV2-UL19-3830 + UGGCCGUGGCCGCGAAGGGCCGCG 24 8213

HSV2-UL19-3831 + CGCCCAGCUCCGGGCCCGG 19 8214

HSV2-U L19-2664 + CCGCCCAGCUCCGGGCCCGG 20 2782

HSV2-UL19-3832 + CCCGCCCAGCUCCGGGCCCGG 21 8215

HSV2-UL19-3833 + UGCCCGCCCAGCUCCGGGCCCGG 23 8216 HSV2-UL19-3834 + CUGCCCGCCCAGCUCCGGGCCCGG 24 8217

HSV2-UL19-2569 + AGG UCCACGU U UCCGCGCGG 20 2718

HSV2-UL19-3835 + CAG G U CCACG U UUCCGCGCGG 21 8218

HSV2-UL19-3836 + CCAGGUCCACGUUUCCGCGCGG 22 8219

HSV2-U L19-3837 + CCCAGGUCCACGUU UCCGCGCGG 23 8220

HSV2-U L19-3838 + ACCC AG G U CCACG U U U CCG CG CG G 24 8221

HSV2-UL19-3839 + CGGGCGCUGCGUAGCAGGG 19 8222

HSV2-U L19-3840 + CGCGGGCGCUGCGUAGCAGGG 21 8223

HSV2-U L19-3841 + UCGCGGGCGCUGCGUAGCAGGG 22 8224

HSV2-UL19-3842 + AUCGCGGGCGCUGCGUAGCAGGG 23 8225

HSV2-U L19-3843 + CAUCGCGGGCGCUGCGUAGCAGGG 24 8226

HSV2-UL19-3844 + CGGGAUGCAGCGGGUGGG 18 8227

HSV2-UL19-3845 + UGGCGGGAUGCAGCGGGUGGG 21 8228

HSV2-U L19-3846 + UUGGCGGGAUGCAGCGGGUGGG 22 8229

HSV2-UL19-3847 + AU UGGCGGGAUGCAGCGGGUGGG 23 8230

HSV2-U L19-3848 + CGGCGAAGCGCUCCAUGG 18 8231

HSV2-U L19-2739 + CGCGGCGAAGCGCUCCAUGG 20 2835

HSV2-UL19-3849 + UGCGCGGCGAAGCGCUCCAUGG 22 8232

HSV2-U L19-3850 + CGUGCGCGGCGAAGCGCUCCAUGG 24 8233

HSV2-UL19-3851 + UGU UGGCCACCAGAU UGG 18 8234

HSV2-UL19-1294 + CGUGUUGGCCACCAGAUUGG 20 1534

HSV2-U L19-3852 + CCGUGUUGGCCACCAGAUUGG 21 8235

HSV2-UL19-3853 + ACCGUGUUGGCCACCAGAUUGG 22 8236

HSV2-UL19-3854 + U G ACCG UG U U GGCCACCAG AU U GG 24 8237

HSV2-U L19-3155 + UGGACGUUGCGCGCGAGCUG 20 7538

HSV2-UL19-3156 + CUGGACGUUGCGCGCGAGCUG 21 7539

HSV2-U L19-3157 + CCUGGACGUUGCGCGCGAGCUG 22 7540

HSV2-U L19-3855 + CGUGCUGCACCACGGGCUG 19 8238

HSV2-UL19-3856 + CGGGCGUGCUGCACCACGGGCUG 23 8239

HSV2-U L19-3857 + CGGGGAGGGAACGCGCGGGGCU 22 8240

HSV2-U L19-3858 + CCGGGGAGGGAACGCGCGGGGCU 23 8241

HSV2-UL19-3859 + CCGCGACG UACGCCCCGU 18 8242

HSV2-U L19-3860 + CGGGGCCGCGACGUACGCCCCGU 23 8243

HSV2-UL19-3861 + CCGGGGCCGCGACGUACGCCCCGU 24 8244

HSV2-UL19-3862 + CGUAGAAG UAUUCGCCAUU 19 8245

HSV2-U L19-2621 + ACGUAGAAGUAU UCGCCAUU 20 2751

HSV2-UL19-3863 + AGGACGUAGAAGUAU UCGCCAUU 23 8246

HSV2-U L19-3864 + CAGGACGUAGAAGUAUUCGCCAUU 24 8247

HSV2-U L19-3865 + CGUUGUCUGGGGGGCGCUU 19 8248

HSV2-UL19-3866 + CGCGUUGUCUGGGGGGCGCUU 21 8249

HSV2-UL19-3867 + CGCGCGU UGUCUGGGGGGCGCUU 23 8250

HSV2-UL19-3868 + ACGCGCGUUGUCUGGGGGGCGCUU 24 8251

HSV2-UL19-3869 - AGCGUCUCAUCGUGGAAA 18 8252

HSV2-U L19-3870 - UGGAGCGUCUCAUCGUGGAAA 21 8253

HSV2-UL19-3871 - CUGG AGCGUCUCAUCGUGGAAA 22 8254

HSV2-UL19-3872 - CCUGG AGCGUCUCAUCGUGGAAA 23 8255 HSV2-UL19-3873 - CGCGCGGGCGGCCGGUCGA 19 8256

HSV2-UL19-375 - ACGCGCGGGCGGCCGGUCGA 20 615

HSV2-UL19-3874 - CACGCGCGGGCGGCCGGUCGA 21 8257

HSV2-UL19-3875 - ACACGCGCGGGCGGCCGGUCGA 22 8258

HSV2-U L19-3876 - CGACACGCGCGGGCGGCCGGUCGA 24 8259

HSV2-U L19-3877 - CAACGGGCGCCUGGCGAC 18 8260

HSV2-UL19-3878 - ACAACGGGCGCCUGGCGAC 19 8261

HSV2-U L19-3879 - CGACAACGGGCGCCUGGCGAC 21 8262

HSV2-U L19-3880 - UCG ACAACGGGCGCCUGGCGAC 22 8263

HSV2-UL19-3881 - CUCG ACAACGGGCGCCUGGCGAC 23 8264

HSV2-U L19-3882 - UCUCGACAACGGGCGCCUGGCGAC 24 8265

HSV2-UL19-3883 - CCGCGCGGAAACGUGGAC 18 8266

HSV2-UL19-2192 - AGCCGCGCGGAAACGUGGAC 20 2475

HSV2-U L19-3884 - CAGCCGCGCGGAAACGUGGAC 21 8267

HSV2-UL19-3885 - CCAGCCGCGCGGAAACGUGGAC 22 8268

HSV2-U L19-3886 - CCCAGCCGCGCGGAAACGUGGAC 23 8269

HSV2-U L19-3887 - ACCCAGCCGCGCGGAAACGUGGAC 24 8270

HSV2-UL19-3888 - UCAAGACGGGCCUCCACC 18 8271

HSV2-U L19-3889 - CUCAAGACGGGCCUCCACC 19 8272

HSV2-UL19-3890 - AGCUCAAGACGGGCCUCCACC 21 8273

HSV2-UL19-3891 - CAGCUCAAGACGGGCCUCCACC 22 8274

HSV2-U L19-3892 - CCAGCUCAAGACGGGCCUCCACC 23 8275

HSV2-UL19-3893 - ACCAGCUCAAGACGGGCCUCCACC 24 8276

HSV2-UL19-3894 - CACGACCCCGUCGAGGCC 18 8277

HSV2-U L19-3895 - CCACGACCCCGUCGAGGCC 19 8278

HSV2-UL19-2077 - ACCACGACCCCGUCGAGGCC 20 2399

HSV2-U L19-3896 - AACCACGACCCCGUCGAGGCC 21 8279

HSV2-U L19-3897 - UGAACCACGACCCCGUCGAGGCC 23 8280

HSV2-UL19-558 - CGGGGGCGCUGCUGCACCGC 20 798

HSV2-U L19-3898 - CGCGGGGGCGCUGCUGCACCGC 22 8281

HSV2-U L19-3899 - UCGCGGGGGCGCUGCUGCACCGC 23 8282

HSV2-UL19-3900 - CUCGCGGGGGCGCUGCUGCACCGC 24 8283

HSV2-U L19-3901 - UGCAGUUUAAGCGCCCGC 18 8284

HSV2-UL19-711 - CGUGCAGU UUAAGCGCCCGC 20 951

HSV2-UL19-3902 - ACG UGCAG U U U AAGCGCCCGC 21 8285

HSV2-U L19-3903 - CGACGUGCAGU UUAAGCGCCCGC 23 8286

HSV2-UL19-3904 - CAGACAACGCGCGUGCGC 18 8287

HSV2-U L19-3905 - CCAGACAACGCGCGUGCGC 19 8288

HSV2-U L19-2044 - CCCAGACAACGCGCGUGCGC 20 2380

HSV2-UL19-3906 - CCCCAGACAACGCGCGUGCGC 21 8289

HSV2-UL19-3907 - CCCCCAGACAACGCGCGUGCGC 22 8290

HSV2-UL19-3908 - CCCCCCAG AC A ACG CGCGUGCGC 23 8291

HSV2-UL19-3909 - CGGGCAGGCUCAGGCCGAG 19 8292

HSV2-U L19-3910 - UGGGCGGGCAGGCUCAGGCCGAG 23 8293

HSV2-UL19-3911 - CUGGGCGGGCAGGCUCAGGCCGAG 24 8294

HSV2-UL19-3912 - CGAGGCGAACCGGGAGACG 19 8295 HSV2-UL19-2041 - UCGAGGCGAACCGGGAGACG 20 2377

HSV2-UL19-3913 - CUCGAGGCGAACCGGGAGACG 21 8296

HSV2-UL19-3914 - ACUCGAGGCGAACCGGGAGACG 22 8297

HSV2-UL19-3915 - AACUCGAGGCGAACCGGGAGACG 23 8298

HSV2-U L19-3916 - CAACUCGAGGCGAACCGGGAGACG 24 8299

HSV2-U L19-3917 - UCGGGGGCGACCUCCCCG 18 8300

HSV2-UL19-3918 - CUCGGGGGCGACCUCCCCG 19 8301

HSV2-UL19-507 - CCUCGGGGGCGACCUCCCCG 20 747

HSV2-U L19-3919 - ACCUCGGGGGCGACCUCCCCG 21 8302

HSV2-UL19-3920 - UACCUCGGGGGCGACCUCCCCG 22 8303

HSV2-U L19-3921 - CUACCUCGGGGGCGACCUCCCCG 23 8304

HSV2-UL19-3922 - CCUACCUCGGGGGCGACCUCCCCG 24 8305

HSV2-UL19-3923 - CGCCCACGGCCGGGUCCG 18 8306

HSV2-U L19-3924 - UCGCCCACGGCCGGGUCCG 19 8307

HSV2-UL19-2083 - CUCGCCCACGGCCGGGUCCG 20 2404

HSV2-U L19-3925 - CCUCGCCCACGGCCGGGUCCG 21 8308

HSV2-U L19-3926 - CGCCUCGCCCACGGCCGGGUCCG 23 8309

HSV2-UL19-2084 - CGGCCGGGUCCGGUGGGUCG 20 2405

HSV2-U L19-3927 - ACGGCCGGGUCCGGUGGGUCG 21 8310

HSV2-UL19-3928 - CACGGCCGGGUCCGGUGGGUCG 22 8311

HSV2-UL19-3929 - CCACGGCCGGGUCCGGUGGGUCG 23 8312

HSV2-U L19-3930 - CCCACGGCCGGGUCCGGUGGGUCG 24 8313

HSV2-UL19-2082 - CGGCAGCGCCUCGCCCACGG 20 2403

HSV2-UL19-3931 - UGGCGGCAGCGCCUCGCCCACGG 23 8314

HSV2-U L19-3932 - CUGGCGGCAGCGCCUCGCCCACGG 24 8315

HSV2-UL19-3933 - CCGCCACGAAACGGGCGG 18 8316

HSV2-U L19-3934 - CCCGCCACGAAACGGGCGG 19 8317

HSV2-U L19-3935 - UGGCCCGCCACGAAACGGGCGG 22 8318

HSV2-UL19-3936 - CGCCGGCAUGGACCACGGG 19 8319

HSV2-U L19-3937 - CGCGCCGGCAUGGACCACGGG 21 8320

HSV2-U L19-3938 - CGGCGCGCCGGCAUGGACCACGGG 24 8321

HSV2-UL19-3939 - UGACCUACGCGCUCAUGG 18 8322

HSV2-U L19-3940 - CUGACCUACGCGCUCAUGG 19 8323

HSV2-UL19-3941 - CGCUGACCUACGCGCUCAUGG 21 8324

HSV2-UL19-3942 - CGCGCUGACCUACGCGCUCAUGG 23 8325

HSV2-U L19-3943 - ACGCGCUGACCUACGCGCUCAUGG 24 8326

HSV2-UL19-3944 - ACCACACC AU CCA AAA U G 18 8327

HSV2-U L19-3945 - U G G ACC AC ACC A U CC A A A AU G 21 8328

HSV2-U L19-3946 - CUGGACCACACCAUCCAAAAUG 22 8329

HSV2-UL19-3947 - CCUGGACCACACCAUCCAAAAUG 23 8330

HSV2-UL19-3948 - ACCUGGACCACACCAUCCAAAAUG 24 8331

HSV2-UL19-3949 - UGGACCGCCACCGAGACUG 19 8332

HSV2-UL19-2128 - CUGGACCGCCACCGAGACUG 20 2435

HSV2-U L19-3950 - CCUGGACCGCCACCGAGACUG 21 8333

HSV2-UL19-3951 - CCCUGGACCGCCACCGAGACUG 22 8334

HSV2-UL19-3952 - CGACGUGGGCCGCCACCUG 19 8335 HSV2-UL19-2031 - ACGACGUGGGCCGCCACCUG 20 2371

HSV2-UL19-3953 - UGGACGACGUGGGCCGCCACCUG 23 8336

HSV2-UL19-3954 - CUGGACGACGUGGGCCGCCACCUG 24 8337

HSV2-UL19-3955 - CGGAGAUCGCCCCCGGUG 18 8338

HSV2-U L19-3956 - CCGGAGAUCGCCCCCGGUG 19 8339

HSV2-UL19-565 - CCCGGAGAUCGCCCCCGGUG 20 805

HSV2-UL19-3957 - UCCCGGAGAUCGCCCCCGGUG 21 8340

HSV2-U L19-3958 - UGGUCCCGGAGAUCGCCCCCGGUG 24 8341

HSV2-U L19-3959 - CGGGCCUCCACCCCGGGU 18 8342

HSV2-UL19-3960 - ACGGGCCUCCACCCCGGGU 19 8343

HSV2-U L19-3961 - AGACGGGCCUCCACCCCGGGU 21 8344

HSV2-UL19-3962 - AAGACGGGCCUCCACCCCGGGU 22 8345

HSV2-UL19-3963 - CAAGACGGGCCUCCACCCCGGGU 23 8346

HSV2-U L19-3964 - U C A AG ACGGGCCUCCACCCCGGGU 24 8347

HSV2-UL19-3965 - ACGGGCAGGAUGCCG UGU 18 8348

HSV2-U L19-3966 - CACGGGCAGGAUGCCGUGU 19 8349

HSV2-U L19-2207 - CCACGGGCAGGAUGCCGUGU 20 2486

HSV2-UL19-3967 - ACCACGGGCAGGAUGCCGUGU 21 8350

HSV2-U L19-3968 - UGGACCACGGGCAGGAUGCCGUGU 24 8351

Table 6G provides exemplary targeting domains for knocking out the UL19 gene selected according to the seven tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 6G

HSV2-UL19-3977 + CCCCCAGGGCCGGGGGGACCA 21 8360

HSV2-UL19-3978 + GCCCCCAGGGCCGGGGGGACCA 22 8361

HSV2-UL19-3979 + GGCCCCCAGGGCCGGGGGGACCA 23 8362

HSV2-UL19-3980 + UGGCCCCCAGGGCCGGGGGGACCA 24 8363

HSV2-UL19-3981 + CUGGCACUCGGCGACCCA 18 8364

HSV2-UL19-3982 + UCUGGCACUCGGCGACCCA 19 8365

HSV2-UL19-2708 + AUCUGGCACUCGGCGACCCA 20 2815

HSV2-UL19-3983 + CAUCUGGCACUCGGCGACCCA 21 8366

HSV2-UL19-3984 + UCAUCUGGCACUCGGCGACCCA 22 8367

HSV2-UL19-3985 + GUCAUCUGGCACUCGGCGACCCA 23 8368

HSV2-UL19-3986 + GGUCAUCUGGCACUCGGCGACCCA 24 8369

HSV2-UL19-3987 + GGGCGUCACGAAACGCCA 18 8370

HSV2-UL19-3988 + CGGGCGUCACGAAACGCCA 19 8371

HSV2-UL19-2687 + CCGGGCGUCACGAAACGCCA 20 2802

HSV2-UL19-3989 + CCCGGGCGUCACGAAACGCCA 21 8372

HSV2-UL19-3990 + CCCCGGGCGUCACGAAACGCCA 22 8373

HSV2-UL19-3991 + GCCCCGGGCGUCACGAAACGCCA 23 8374

HSV2-UL19-3992 + GGCCCCGGGCGUCACGAAACGCCA 24 8375

HSV2-UL19-3993 + GCUGGUGAUACAGGGCCA 18 8376

HSV2-UL19-3994 + AGCUGGUGAUACAGGGCCA 19 8377

HSV2-UL19-1230 + GAGCUGGUGAUACAGGGCCA 20 1470

HSV2-UL19-3995 + UGAGCUGGUGAUACAGGGCCA 21 8378

HSV2-UL19-3996 + UUGAGCUGGUGAUACAGGGCCA 22 8379

HSV2-UL19-3997 + CU UGAGCUGGUGAUACAGGGCCA 23 8380

HSV2-UL19-3998 + UCUUGAGCUGGUGAUACAGGGCCA 24 8381

HSV2-UL19-3999 + U G G G CAACAG CAG G G CCA 18 8382

HSV2-UL19-4000 + AU G G G CAACAG CAG G G CCA 19 8383

HSV2-UL19-1541 + CAU G G G CAACAG C AG G G CCA 20 1781

HSV2-UL19-4001 + G CAU G G G CAACAG CAG G G CCA 21 8384

HSV2-UL19-4002 + U G CAU G G G CAACAG CAG GG CCA 22 8385

HSV2-UL19-4003 + UUGCAUGGG CAACAG CAG G G CCA 23 8386

HSV2-UL19-4004 + GUUGCAUGGGCAACAGCAGGGCCA 24 8387

HSV2-UL19-4005 + AGU UGAUGUCCGUGGCCA 18 8388

HSV2-UL19-4006 + UAGUUGAUGUCCGUGGCCA 19 8389

HSV2-UL19-1165 + GUAGUUGAUGUCCGUGGCCA 20 1405

HSV2-UL19-4007 + AGU AGUUGAUGUCCGUGGCCA 21 8390

HSV2-UL19-4008 + AAG UAGUUGAUGUCCGUGGCCA 22 8391

HSV2-UL19-4009 + AAAGU AGUUGAUGUCCGUGGCCA 23 8392

HSV2-UL19-4010 + GAAAGUAGUUGAUGUCCGUGGCCA 24 8393

HSV2-UL19-4011 + CGGCCGCGUUGUCUAGCA 18 8394

HSV2-UL19-4012 + GCGGCCGCGUUGUCUAGCA 19 8395

HSV2-UL19-2555 + GGCGGCCGCGUUGUCUAGCA 20 2713

HSV2-UL19-4013 + CGGCGGCCGCGUUGUCUAGCA 21 8396

HSV2-UL19-4014 + ACGGCGGCCGCGUUGUCUAGCA 22 8397

HSV2-UL19-4015 + CACGGCGGCCGCGUUGUCUAGCA 23 8398

HSV2-UL19-4016 + ACACGGCGGCCGCGUUGUCUAGCA 24 8399 HSV2-UL19-4017 + UCGCUCGGGCACUCCUCA 18 8400

HSV2-UL19-4018 + AUCGCUCGGGCACUCCUCA 19 8401

HSV2-UL19-2644 + GAUCGCUCGGGCACUCCUCA 20 2766

HSV2-UL19-4019 + GGAUCGCUCGGGCACUCCUCA 21 8402

HSV2-UL19-4020 + GGGAUCGCUCGGGCACUCCUCA 22 8403

HSV2-UL19-4021 + CGGGAUCGCUCGGGCACUCCUCA 23 8404

HSV2-UL19-4022 + ACGGGAUCGCUCGGGCACUCCUCA 24 8405

HSV2-UL19-4023 + CCUGGGCGCAGCCAAAGA 18 8406

HSV2-UL19-4024 + ACCUGGGCGCAGCCAAAGA 19 8407

HSV2-UL19-2549 + CACCUGGGCGCAGCCAAAGA 20 2710

HSV2-UL19-4025 + GCACCUGGGCGCAGCCAAAGA 21 8408

HSV2-UL19-4026 + GGCACCUGGGCGCAG CCAAAG A 22 8409

HSV2-UL19-4027 + CGGCACCUGGGCGCAGCCAAAGA 23 8410

HSV2-UL19-4028 + GCGGCACCUGGGCGCAGCCAAAGA 24 8411

HSV2-UL19-4029 + GCACCACGGGCUGGCGGA 18 8412

HSV2-UL19-4030 + UGCACCACGGGCUGGCGGA 19 8413

HSV2-UL19-1240 + CUGCACCACGGGCUGGCGGA 20 1480

HSV2-UL19-4031 + GCUGCACCACGGGCUGGCGGA 21 8414

HSV2-UL19-4032 + UGCUGCACCACGGGCUGGCGGA 22 8415

HSV2-UL19-4033 + GUGCUGCACCACGGGCUGGCGGA 23 8416

HSV2-UL19-4034 + CGUGCUGCACCACGGGCUGGCGGA 24 8417

HSV2-UL19-4035 + AAAAACAG CUGCCGGGGA 18 8418

HSV2-UL19-4036 + AAAAAACAGCUGCCGGGGA 19 8419

HSV2-UL19-1450 + AAAAAAACAG CUGCCGGGGA 20 1690

HSV2-UL19-4037 + CAAAAAAACAGCUGCCGGGGA 21 8420

HSV2-UL19-4038 + CCAAAAAAACAG CU G CCG G G G A 22 8421

HSV2-UL19-4039 + CCCAAAAA AACAG CU G CCG G G G A 23 8422

HSV2-UL19-4040 + CCCCAAAAAAACAG CU G CCG G G G A 24 8423

HSV2-UL19-4041 + CCGCGUCGAUGUUCAUGA 18 8424

HSV2-UL19-4042 + GCCGCGUCGAUGUUCAUGA 19 8425

HSV2-UL19-2720 + GGCCGCGUCGAUGUUCAUGA 20 2824

HSV2-UL19-4043 + CGGCCGCGUCGAUGUUCAUGA 21 8426

HSV2-UL19-4044 + ACGGCCGCGUCGAUGUUCAUGA 22 8427

HSV2-UL19-4045 + GACGGCCGCGUCGAUGUUCAUGA 23 8428

HSV2-UL19-4046 + CGACGGCCGCGUCGAUGUUCAUGA 24 8429

HSV2-UL19-4047 + CGUCUUGAGCUGGUGAUA 18 8430

HSV2-UL19-4048 + CCGUCUUGAGCUGGUGAUA 19 8431

HSV2-UL19-2586 + CCCGUCUUGAGCUGGUGAUA 20 2731

HSV2-UL19-4049 + GCCCGUCUUGAGCUGGUGAUA 21 8432

HSV2-UL19-4050 + GGCCCGUCUUGAGCUGGUGAUA 22 8433

HSV2-UL19-4051 + AGGCCCGUCUUGAGCUGGUGAUA 23 8434

HSV2-UL19-4052 + GAGGCCCGUCUUGAGCUGGUGAUA 24 8435

HSV2-UL19-4053 + AGGUUGCCCAUGUCCGUA 18 8436

HSV2-UL19-4054 + GAGGUUGCCCAUGUCCGUA 19 8437

HSV2-UL19-2566 + GGAGGUUGCCCAUGUCCGUA 20 2716

HSV2-UL19-4055 + GGG AGGUUGCCCAUGUCCGUA 21 8438 HSV2-UL19-4056 + GGGGAGGUUGCCCAUGUCCGUA 22 8439

HSV2-UL19-4057 + GGGGGAGGUUGCCCAUGUCCGUA 23 8440

HSV2-UL19-4058 + UGGGGGAGGUUGCCCAUGUCCGUA 24 8441

HSV2-UL19-4059 + ACAG AGACAGGCCCU UU A 18 8442

HSV2-UL19-4060 + UACAGAGACAGGCCCUUUA 19 8443

HSV2-UL19-2505 + UUACAGAGACAGGCCCUUUA 20 2673

HSV2-UL19-4061 + UUUACAGAGACAGGCCCUUUA 21 8444

HSV2-UL19-4062 + U U U U ACAGAGACAGGCCCU U U A 22 8445

HSV2-UL19-4063 + AUUUUACAGAGACAGGCCCUUUA 23 8446

HSV2-UL19-4064 + AAU U U UACAG AGACAGGCCCU U U A 24 8447

HSV2-UL19-4065 + AAGAAGAAGCCAAUUUUA 18 8448

HSV2-UL19-4066 + GAAGAAGAAGCCAAUUUUA 19 8449

HSV2-UL19-4067 + GGAAGAAGAAGCCAAUUUUA 20 8450

HSV2-UL19-4068 + GGGAAGAAGAAGCCAAUUUUA 21 8451

HSV2-UL19-4069 + GGGGAAGAAGAAGCCAAUUUUA 22 8452

HSV2-UL19-4070 + UGGGGAAGAAGAAGCCAAUUUUA 23 8453

HSV2-UL19-4071 + CUGGGGAAGAAGAAGCCAAUUUUA 24 8454

HSV2-UL19-4072 + UCGGCCGCGGUGAAGAAC 18 8455

HSV2-UL19-4073 + GUCGGCCGCGGUGAAGAAC 19 8456

HSV2-UL19-2525 + UG UCGGCCGCGGUGAAGAAC 20 2689

HSV2-UL19-4074 + AU GUCGGCCGCGGUGAAGAAC 21 8457

HSV2-UL19-4075 + GAUG UCGGCCGCGGUGAAGAAC 22 8458

HSV2-UL19-4076 + UGAUGUCGGCCGCGGUGAAGAAC 23 8459

HSV2-UL19-4077 + GUGAUGUCGGCCGCGGUGAAGAAC 24 8460

HSV2-UL19-4078 + ACGACGGUGAACCCGAAC 18 8461

HSV2-UL19-4079 + CACGACGGUGAACCCGAAC 19 8462

HSV2-UL19-2581 + GCACGACGGUGAACCCGAAC 20 2728

HSV2-UL19-4080 + CGCACGACGGUGAACCCGAAC 21 8463

HSV2-UL19-4081 + CCGCACGACGGUGAACCCGAAC 22 8464

HSV2-UL19-4082 + GCCGCACGACGGUGAACCCGAAC 23 8465

HSV2-UL19-4083 + UGCCGCACGACGGUGAACCCGAAC 24 8466

HSV2-UL19-4084 + CCCGCGAAGAAGUCGAAC 18 8467

HSV2-UL19-4085 + GCCCGCGAAGAAGUCGAAC 19 8468

HSV2-UL19-2703 + CGCCCGCGAAGAAGUCGAAC 20 2811

HSV2-UL19-4086 + ACGCCCGCGAAGAAGUCGAAC 21 8469

HSV2-UL19-4087 + CACGCCCGCGAAGAAGUCGAAC 22 8470

HSV2-UL19-4088 + CCACGCCCGCGAAGAAGUCGAAC 23 8471

HSV2-UL19-4089 + GCCACGCCCGCGAAGAAGUCGAAC 24 8472

HSV2-UL19-4090 + UGCCGCACGACGGUGAAC 18 8473

HSV2-UL19-4091 + CUGCCGCACGACGGUGAAC 19 8474

HSV2-UL19-2580 + CCUGCCGCACGACGGUGAAC 20 2727

HSV2-UL19-4092 + UCCUGCCGCACGACGGUGAAC 21 8475

HSV2-UL19-4093 + GUCCUGCCGCACGACGGUGAAC 22 8476

HSV2-UL19-4094 + GGUCCUGCCGCACGACGGUGAAC 23 8477

HSV2-UL19-4095 + CGGUCCUGCCGCACGACGGUGAAC 24 8478

HSV2-UL19-4096 + GCCGUCGCAGUCCCACAC 18 8479 HSV2-UL19-4097 + GGCCGUCGCAGUCCCACAC 19 8480

HSV2-UL19-2659 + AGGCCGUCGCAGUCCCACAC 20 2778

HSV2-UL19-4098 + AAGGCCGUCGCAGUCCCACAC 21 8481

HSV2-UL19-4099 + UAAGGCCGUCGCAGUCCCACAC 22 8482

HSV2-UL19-4100 + AUAAGGCCGUCGCAGUCCCACAC 23 8483

HSV2-UL19-4101 + CAUAAGGCCGUCGCAGUCCCACAC 24 8484

HSV2-UL19-4102 + CAGGUCCAUGGCGCCCAC 18 8485

HSV2-UL19-4103 + UCAGGUCCAUGGCGCCCAC 19 8486

HSV2-UL19-2745 + GUCAGGUCCAUGGCGCCCAC 20 2840

HSV2-UL19-4104 + CGUCAGGUCCAUGGCGCCCAC 21 8487

HSV2-UL19-4105 + ACGUCAGGUCCAUGGCGCCCAC 22 8488

HSV2-UL19-4106 + AACGUCAGGUCCAUGGCGCCCAC 23 8489

HSV2-UL19-4107 + GAACGUCAGGUCCAUGGCGCCCAC 24 8490

HSV2-UL19-4108 + AGGCCGUCGCAGUCCCAC 18 8491

HSV2-UL19-4109 + AAGGCCGUCGCAGUCCCAC 19 8492

HSV2-UL19-2658 + UAAGGCCGUCGCAGUCCCAC 20 2777

HSV2-UL19-4110 + AUAAGGCCGUCGCAGUCCCAC 21 8493

HSV2-UL19-4111 + CAUAAGGCCGUCGCAGUCCCAC 22 8494

HSV2-UL19-4112 + GCAUAAGGCCGUCGCAGUCCCAC 23 8495

HSV2-UL19-4113 + CGCAUAAGGCCGUCGCAGUCCCAC 24 8496

HSV2-UL19-4114 + CGUUUCGUGGCGGGCCAC 18 8497

HSV2-UL19-4115 + CCGUUUCGUGGCGGGCCAC 19 8498

HSV2-UL19-2573 + CCCGUUUCGUGGCGGGCCAC 20 2722

HSV2-UL19-4116 + GCCCGUUUCGUGGCGGGCCAC 21 8499

HSV2-UL19-4117 + CGCCCGUUUCGUGGCGGGCCAC 22 8500

HSV2-UL19-4118 + CCGCCCGUUUCGUGGCGGGCCAC 23 8501

HSV2-UL19-4119 + CCCGCCCGUUUCGUGGCGGGCCAC 24 8502

HSV2-UL19-4120 + GCCGGCGCGCCGCGGCAC 18 8503

HSV2-UL19-4121 + UGCCGGCGCGCCGCGGCAC 19 8504

HSV2-UL19-2548 + AUGCCGGCGCGCCGCGGCAC 20 2709

HSV2-UL19-4122 + CAUGCCGGCGCGCCGCGGCAC 21 8505

HSV2-UL19-4123 + CCAUGCCGGCGCGCCGCGGCAC 22 8506

HSV2-UL19-4124 + UCCAUGCCGGCGCGCCGCGGCAC 23 8507

HSV2-UL19-4125 + GUCCAUGCCGGCGCGCCGCGGCAC 24 8508

HSV2-UL19-4126 + CGCUCGGGCACUCCUCAC 18 8509

HSV2-UL19-4127 + UCGCUCGGGCACUCCUCAC 19 8510

HSV2-UL19-1308 + AUCGCUCGGGCACUCCUCAC 20 1548

HSV2-UL19-4128 + GAUCGCUCGGGCACUCCUCAC 21 8511

HSV2-UL19-4129 + GGAUCGCUCGGGCACUCCUCAC 22 8512

HSV2-UL19-4130 + GGGAUCGCUCGGGCACUCCUCAC 23 8513

HSV2-UL19-4131 + CGGGAUCGCUCGGGCACUCCUCAC 24 8514

HSV2-UL19-4132 + CUGGGCGCAGCCAAAGAC 18 8515

HSV2-UL19-4133 + CCUGGGCGCAGCCAAAGAC 19 8516

HSV2-UL19-1176 + ACCUGGGCGCAGCCAAAGAC 20 1416

HSV2-UL19-4134 + CACCUGGGCGCAGCCAAAGAC 21 8517

HSV2-UL19-4135 + GCACCUGGGCGCAGCCAAAGAC 22 8518 HSV2-UL19-4136 + GGCACCUGGGCGCAG CCAAAG AC 23 8519

HSV2-UL19-4137 + CGGCACCUGGGCGCAGCCAAAGAC 24 8520

HSV2-UL19-4138 + CUGGGCCAGGGCCUCGAC 18 8521

HSV2-UL19-4139 + GCUGGGCCAGGGCCUCGAC 19 8522

HSV2-UL19-2667 + AGCUGGGCCAGGGCCUCGAC 20 2785

HSV2-UL19-4140 + CAGCUGGGCCAGGGCCUCGAC 21 8523

HSV2-UL19-4141 + CCAGCUGGGCCAGGGCCUCGAC 22 8524

HSV2-UL19-4142 + ACCAGCUGGGCCAGGGCCUCGAC 23 8525

HSV2-UL19-4143 + CACCAGCUGGGCCAGGGCCUCGAC 24 8526

HSV2-UL19-4144 + CCCCAGGGCCGGGGGGAC 18 8527

HSV2-UL19-4145 + CCCCCAGGGCCGGGGGGAC 19 8528

HSV2-UL19-2605 + GCCCCCAGGGCCGGGGGGAC 20 2743

HSV2-UL19-4146 + GGCCCCCAGGGCCGGGGGGAC 21 8529

HSV2-UL19-4147 + UGGCCCCCAGGGCCGGGGGGAC 22 8530

HSV2-UL19-4148 + UU GGCCCCCAGGGCCGGGGGGAC 23 8531

HSV2-UL19-4149 + GUUGGCCCCCAGGGCCGGGGGGAC 24 8532

HSV2-UL19-4150 + UACAGCAGGUCCCCGUAC 18 8533

HSV2-UL19-4151 + GUACAGCAGGUCCCCGUAC 19 8534

HSV2-UL19-2534 + UGUACAGCAGGUCCCCGUAC 20 2697

HSV2-UL19-4152 + UUGUACAGCAGGUCCCCGUAC 21 8535

HSV2-UL19-4153 + GUUGUACAGCAGGUCCCCGUAC 22 8536

HSV2-UL19-4154 + CGUUGUACAGCAGGUCCCCGUAC 23 8537

HSV2-UL19-4155 + CCGUUGUACAGCAGGUCCCCGUAC 24 8538

HSV2-UL19-4156 + CUCUGGCCGUGGUCGUAC 18 8539

HSV2-UL19-4157 + GCUCUGGCCGUGGUCGUAC 19 8540

HSV2-UL19-2539 + CGCUCUGGCCGUGGUCGUAC 20 2702

HSV2-UL19-4158 + UCGCUCUGGCCGUGGUCGUAC 21 8541

HSV2-UL19-4159 + GUCGCUCUGGCCGUGGUCGUAC 22 8542

HSV2-UL19-4160 + GGUCGCUCUGGCCGUGGUCGUAC 23 8543

HSV2-UL19-4161 + GGGUCGCUCUGGCCGUGGUCGUAC 24 8544

HSV2-UL19-4162 + AGGUCCAUGGCGCCCACC 18 8545

HSV2-UL19-4163 + CAGGUCCAUGGCGCCCACC 19 8546

HSV2-UL19-1475 + UCAGGUCCAUGGCGCCCACC 20 1715

HSV2-UL19-4164 + GUCAGGUCCAUGGCGCCCACC 21 8547

HSV2-UL19-4165 + CGUCAGGUCCAUGGCGCCCACC 22 8548

HSV2-UL19-4166 + ACGUCAGGUCCAUGGCGCCCACC 23 8549

HSV2-UL19-4167 + AACGUCAGGUCCAUGGCGCCCACC 24 8550

HSV2-UL19-4168 + GUUUCGUGGCGGGCCACC 18 8551

HSV2-UL19-4169 + CGUU UCGUGGCGGGCCACC 19 8552

HSV2-UL19-1216 + CCGUUUCGUGGCGGGCCACC 20 1456

HSV2-UL19-4170 + CCCGUUUCGUGGCGGGCCACC 21 8553

HSV2-UL19-4171 + GCCCGUUUCGUGGCGGGCCACC 22 8554

HSV2-UL19-4172 + CGCCCGUUUCGUGGCGGGCCACC 23 8555

HSV2-UL19-4173 + CCGCCCGUUUCGUGGCGGGCCACC 24 8556

HSV2-UL19-4174 + UCGCGGGCGUGCUGCACC 18 8557

HSV2-UL19-4175 + CUCGCGGGCGUGCUGCACC 19 8558 HSV2-UL19-2592 + UCUCGCGGGCGUGCUGCACC 20 2736

HSV2-UL19-4176 + CUCUCGCGGGCGUGCUGCACC 21 8559

HSV2-UL19-4177 + GCUCUCGCGGGCGUGCUGCACC 22 8560

HSV2-UL19-4178 + CGCUCUCGCGGGCGUGCUGCACC 23 8561

HSV2-UL19-4179 + GCGCUCUCGCGGGCGUGCUGCACC 24 8562

HSV2-UL19-4180 + GCUCGGGCACUCCUCACC 18 8563

HSV2-UL19-4181 + CGCUCGGGCACUCCUCACC 19 8564

HSV2-UL19-1309 + UCGCUCGGGCACUCCUCACC 20 1549

HSV2-UL19-4182 + AUCGCUCGGGCACUCCUCACC 21 8565

HSV2-UL19-4183 + GAUCGCUCGGGCACUCCUCACC 22 8566

HSV2-UL19-4184 + GGAUCGCUCGGGCACUCCUCACC 23 8567

HSV2-UL19-4185 + GGGAUCGCUCGGGCACUCCUCACC 24 8568

HSV2-UL19-4186 + UGGGCGCAGCCAAAGACC 18 8569

HSV2-UL19-4187 + CUGGGCGCAGCCAAAGACC 19 8570

HSV2-UL19-1177 + CCUGGGCGCAGCCAAAGACC 20 1417

HSV2-UL19-4188 + ACCUGGGCGCAGCCAAAGACC 21 8571

HSV2-UL19-4189 + CACCUGGGCGCAGCCAAAGACC 22 8572

HSV2-UL19-4190 + GCACCUGGGCGCAGCCAAAGACC 23 8573

HSV2-UL19-4191 + GGCACCUGGGCGCAG CCAAAG ACC 24 8574

HSV2-UL19-4192 + CCCAGGGCCGGGGGGACC 18 8575

HSV2-UL19-4193 + CCCCAGGGCCGGGGGGACC 19 8576

HSV2-UL19-1250 + CCCCCAGGGCCGGGGGGACC 20 1490

HSV2-UL19-4194 + GCCCCCAGGGCCGGGGGGACC 21 8577

HSV2-UL19-4195 + GGCCCCCAGGGCCGGGGGGACC 22 8578

HSV2-UL19-4196 + UGGCCCCCAGGGCCGGGGGGACC 23 8579

HSV2-UL19-4197 + UUGGCCCCCAGGGCCGGGGGGACC 24 8580

HSV2-UL19-4198 + CGGUGCAGCAGCGCCCCC 18 8581

HSV2-UL19-4199 + GCGGUGCAGCAGCGCCCCC 19 8582

HSV2-UL19-2651 + CGCGGUGCAGCAGCGCCCCC 20 2770

HSV2-UL19-4200 + CCGCGGUGCAGCAGCGCCCCC 21 8583

HSV2-UL19-4201 + CCCGCGGUGCAGCAGCGCCCCC 22 8584

HSV2-UL19-4202 + CCCCGCGGUGCAGCAGCGCCCCC 23 8585

HSV2-UL19-4203 + CCCCCGCGGUGCAGCAGCGCCCCC 24 8586

HSV2-UL19-4204 + GCCGGGUUGAACAGCCCC 18 8587

HSV2-UL19-4205 + GGCCGGGUUGAACAGCCCC 19 8588

HSV2-UL19-1469 + UGGCCGGGUUGAACAGCCCC 20 1709

HSV2-UL19-4206 + AU GGCCGGGUUGAACAGCCCC 21 8589

HSV2-UL19-4207 + CAUGGCCGGGUUGAACAGCCCC 22 8590

HSV2-UL19-4208 + CCAUGGCCGGGUUGAACAGCCCC 23 8591

HSV2-UL19-4209 + UCCAUGGCCGGGUUGAACAGCCCC 24 8592

HSV2-UL19-4210 + GCCGCGCUCAAACGCCCC 18 8593

HSV2-UL19-4211 + UGCCGCGCUCAAACGCCCC 19 8594

HSV2-UL19-2780 + GUGCCGCGCUCAAACGCCCC 20 2862

HSV2-UL19-4212 + CGUGCCGCGCUCAAACGCCCC 21 8595

HSV2-UL19-4213 + CCG U GCCG CGCU CAAACG CCCC 22 8596

HSV2-UL19-4214 + GCCGUGCCGCGCUCAAACGCCCC 23 8597 HSV2-UL19-4215 + GGCCGUGCCGCGCUCAAACGCCCC 24 8598

HSV2-UL19-4216 + GCGGUCCUCGAACGCCCC 18 8599

HSV2-UL19-4217 + UGCGGUCCUCGAACGCCCC 19 8600

HSV2-UL19-2683 + CUGCGGUCCUCGAACGCCCC 20 2798

HSV2-UL19-4218 + GCUGCGGUCCUCGAACGCCCC 21 8601

HSV2-UL19-4219 + AGCUGCGGUCCUCGAACGCCCC 22 8602

HSV2-UL19-4220 + UAGCUGCGGUCCUCGAACGCCCC 23 8603

HSV2-UL19-4221 + GUAGCUGCGGUCCUCGAACGCCCC 24 8604

HSV2-UL19-4222 + GGAGAAGUAGUUGGCCCC 18 8605

HSV2-UL19-4223 + AGGAGAAGUAGUUGGCCCC 19 8606

HSV2-UL19-2599 + GAGGAGAAGUAGUUGGCCCC 20 2741

HSV2-UL19-4224 + GG AGGAGAAGUAGUUGGCCCC 21 8607

HSV2-UL19-4225 + UGGAGGAGAAGUAGUUGGCCCC 22 8608

HSV2-UL19-4226 + AUGGAGGAGAAGUAGUUGGCCCC 23 8609

HSV2-UL19-4227 + GAUGGAGGAGAAGUAGUUGGCCCC 24 8610

HSV2-UL19-4228 + GGCCGGGUUGAACAGCCC 18 8611

HSV2-UL19-4229 + UGGCCGGGUUGAACAGCCC 19 8612

HSV2-UL19-2741 + AUGGCCGGGUUGAACAGCCC 20 2837

HSV2-UL19-4230 + CAUGGCCGGGU UGAACAGCCC 21 8613

HSV2-UL19-4231 + CCAUGGCCGGGUUGAACAGCCC 22 8614

HSV2-UL19-4232 + U CCAU GG CCG GG U U G AACAGCCC 23 8615

HSV2-UL19-4233 + CUCCAUGGCCGGGU UGAACAGCCC 24 8616

HSV2-UL19-4234 + UCGACGUCGGCCACGCCC 18 8617

HSV2-UL19-4235 + CUCGACGUCGGCCACGCCC 19 8618

HSV2-UL19-2700 + GCUCGACGUCGGCCACGCCC 20 2808

HSV2-UL19-4236 + AGCUCGACGUCGGCCACGCCC 21 8619

HSV2-UL19-4237 + AAGCUCGACGUCGGCCACGCCC 22 8620

HSV2-UL19-4238 + GAAGCUCGACGUCGGCCACGCCC 23 8621

HSV2-UL19-4239 + GGAAGCUCGACGUCGGCCACGCCC 24 8622

HSV2-UL19-4240 + AGGUGGCCUGGAUCGCCC 18 8623

HSV2-UL19-4241 + CAGGUGGCCUGGAUCGCCC 19 8624

HSV2-UL19-2691 + CCAGGUGGCCUGGAUCGCCC 20 2805

HSV2-UL19-4242 + GCCAGGUGGCCUGGAUCGCCC 21 8625

HSV2-UL19-4243 + CGCCAGGUGGCCUGGAUCGCCC 22 8626

HSV2-UL19-4244 + GCGCCAGGUGGCCUGGAUCGCCC 23 8627

HSV2-UL19-4245 + CGCGCCAGGUGGCCUGGAUCGCCC 24 8628

HSV2-UL19-4246 + UCCUCGGGGAGGUCGCCC 18 8629

HSV2-UL19-4247 + CUCCUCGGGGAGGUCGCCC 19 8630

HSV2-UL19-2672 + ACUCCUCGGGGAGGUCGCCC 20 2787

HSV2-UL19-4248 + CACUCCUCGGGGAGGUCGCCC 21 8631

HSV2-UL19-4249 + GCACUCCUCGGGGAGGUCGCCC 22 8632

HSV2-UL19-4250 + UGCACUCCUCGGGGAGGUCGCCC 23 8633

HSV2-UL19-4251 + AUGCACUCCUCGGGGAGGUCGCCC 24 8634

HSV2-UL19-4252 + CGAUGGCCCGCCUUGCCC 18 8635

HSV2-UL19-4253 + GCGAUGGCCCGCCUUGCCC 19 8636

HSV2-UL19-1528 + GGCGAUGGCCCGCCUUGCCC 20 1768 HSV2-UL19-4254 + CGGCGAUGGCCCGCCUUGCCC 21 8637

HSV2-UL19-4255 + GCGGCGAUGGCCCGCCUUGCCC 22 8638

HSV2-UL19-4256 + CGCGGCGAUGGCCCGCCUUGCCC 23 8639

HSV2-UL19-4257 + UCGCGGCGAUGGCCCGCCUUGCCC 24 8640

HSV2-UL19-4258 + AUCUUGAAGUACCCCGCC 18 8641

HSV2-UL19-4259 + CAUCUUGAAGUACCCCGCC 19 8642

HSV2-UL19-2590 + UCAUCU UGAAGUACCCCGCC 20 2734

HSV2-UL19-4260 + CUCAUCUUGAAGUACCCCGCC 21 8643

HSV2-UL19-4261 + GCUCAUCU UGAAGUACCCCGCC 22 8644

HSV2-UL19-4262 + GGCUCAUCUUGAAGUACCCCGCC 23 8645

HSV2-UL19-4263 + GGGCUCAUCUUGAAGUACCCCGCC 24 8646

HSV2-UL19-4264 + CGGGGGGACUUCGCCGCC 18 8647

HSV2-UL19-4265 + CCGGGGGGACUUCGCCGCC 19 8648

HSV2-UL19-1409 + GCCGGGGGGACUUCGCCGCC 20 1649

HSV2-UL19-4266 + GGCCGGGGGGACUUCGCCGCC 21 8649

HSV2-UL19-4267 + CGGCCGGGGGGACU UCGCCGCC 22 8650

HSV2-UL19-4268 + CCGGCCGGGGGGACUUCGCCGCC 23 8651

HSV2-UL19-4269 + ACCGGCCGGGGGGACU UCGCCGCC 24 8652

HSV2-UL19-4270 + GCCACCGGACACAGCGCC 18 8653

HSV2-UL19-4271 + CGCCACCGGACACAGCGCC 19 8654

HSV2-UL19-1394 + ACGCCACCGGACACAGCGCC 20 1634

HSV2-UL19-4272 + AACGCCACCGGACACAGCGCC 21 8655

HSV2-UL19-4273 + AAACG CCACCG G ACACAGCG CC 22 8656

HSV2-UL19-4274 + GAAACGCCACCGGACACAGCGCC 23 8657

HSV2-UL19-4275 + CGAAACGCCACCGGACACAGCGCC 24 8658

HSV2-UL19-4276 + GCGCCCGCGUCGGGCGCC 18 8659

HSV2-UL19-4277 + GGCGCCCGCGUCGGGCGCC 19 8660

HSV2-UL19-2629 + UGGCGCCCGCGUCGGGCGCC 20 2756

HSV2-UL19-4278 + UUGGCGCCCGCGUCGGGCGCC 21 8661

HSV2-UL19-4279 + GUUGGCGCCCGCGUCGGGCGCC 22 8662

HSV2-UL19-4280 + UGUUGGCGCCCGCGUCGGGCGCC 23 8663

HSV2-UL19-4281 + GUGUUGGCGCCCGCGUCGGGCGCC 24 8664

HSV2-UL19-4282 + AACGCCGAGCUCCAGGCC 18 8665

HSV2-UL19-4283 + CAACGCCGAGCUCCAGGCC 19 8666

HSV2-UL19-2685 + CCAACGCCGAGCUCCAGGCC 20 2800

HSV2-UL19-4284 + GCCAACGCCGAGCUCCAGGCC 21 8667

HSV2-UL19-4285 + GGCCAACGCCGAGCUCCAGGCC 22 8668

HSV2-UL19-4286 + CGGCCAACGCCGAGCUCCAGGCC 23 8669

HSV2-UL19-4287 + GCGGCCAACGCCGAGCUCCAGGCC 24 8670

HSV2-UL19-4288 + GUCAGGCGGGGCACGGCC 18 8671

HSV2-UL19-4289 + CGUCAGGCGGGGCACGGCC 19 8672

HSV2-UL19-2766 + GCGUCAGGCGGGGCACGGCC 20 2852

HSV2-UL19-4290 + UGCGUCAGGCGGGGCACGGCC 21 8673

HSV2-UL19-4291 + GUGCGUCAGGCGGGGCACGGCC 22 8674

HSV2-UL19-4292 + CGUGCGUCAGGCGGGGCACGGCC 23 8675

HSV2-UL19-4293 + GCGUGCGUCAGGCGGGGCACGGCC 24 8676 HSV2-UL19-4294 + AUCGCCCCCGGACCGGCC 18 8677

HSV2-UL19-4295 + GAUCGCCCCCGGACCGGCC 19 8678

HSV2-UL19-1404 + GGAUCGCCCCCGGACCGGCC 20 1644

HSV2-UL19-4296 + UGGAUCGCCCCCGGACCGGCC 21 8679

HSV2-UL19-4297 + CUGGAUCGCCCCCGGACCGGCC 22 8680

HSV2-UL19-4298 + CCUGGAUCGCCCCCGGACCGGCC 23 8681

HSV2-UL19-4299 + GCCUGGAUCGCCCCCGGACCGGCC 24 8682

HSV2-UL19-4300 + GACCCACCGGACCCGGCC 18 8683

HSV2-UL19-4301 + CGACCCACCGGACCCGGCC 19 8684

HSV2-UL19-2709 + GCGACCCACCGGACCCGGCC 20 2816

HSV2-UL19-4302 + GGCGACCCACCGGACCCGGCC 21 8685

HSV2-UL19-4303 + CGGCGACCCACCGGACCCGGCC 22 8686

HSV2-UL19-4304 + UCGGCGACCCACCGGACCCGGCC 23 8687

HSV2-UL19-4305 + CUCGGCGACCCACCGGACCCGGCC 24 8688

HSV2-UL19-4306 + AGCUGGUGAUACAGGGCC 18 8689

HSV2-UL19-4307 + GAGCUGGUGAUACAGGGCC 19 8690

HSV2-UL19-2587 + UGAGCUGGUGAUACAGGGCC 20 2732

HSV2-UL19-4308 + UU GAGCUGGUGAUACAGGGCC 21 8691

HSV2-UL19-4309 + CU UGAGCUGGUGAUACAGGGCC 22 8692

HSV2-UL19-4310 + UCU UGAGCUGGUGAUACAGGGCC 23 8693

HSV2-UL19-4311 + GUCUUGAGCUGGUGAUACAGGGCC 24 8694

HSV2-UL19-4312 + UAGUUGGCCCCCAGGGCC 18 8695

HSV2-UL19-4313 + GUAGUUGGCCCCCAGGGCC 19 8696

HSV2-UL19-1246 + AGUAGUUGGCCCCCAGGGCC 20 1486

HSV2-UL19-4314 + AAGUAGUUGGCCCCCAGGGCC 21 8697

HSV2-UL19-4315 + GAAGUAGUUGGCCCCCAGGGCC 22 8698

HSV2-UL19-4316 + AG AAGUAGUUGGCCCCCAGGGCC 23 8699

HSV2-UL19-4317 + GAGAAGUAGUUGGCCCCCAGGGCC 24 8700

HSV2-UL19-4318 + AUGGGCAACAGCAGGGCC 18 8701

HSV2-UL19-4319 + CAU G G G CAACAG CAG G G CC 19 8702

HSV2-UL19-1540 + GCAUGGGCAACAGCAGGGCC 20 1780

HSV2-UL19-4320 + UGCAUGGGCAACAGCAGGGCC 21 8703

HSV2-UL19-4321 + UUGCAUGGGCAACAGCAGGGCC 22 8704

HSV2-UL19-4322 + GU UGCAUGGGCAACAGCAGGGCC 23 8705

HSV2-UL19-4323 + CGUUGCAUGGGCAACAGCAGGGCC 24 8706

HSV2-UL19-4324 + GCCAGGUCGCGCAGGGCC 18 8707

HSV2-UL19-4325 + CGCCAGGUCGCGCAGGGCC 19 8708

HSV2-UL19-1258 + GCGCCAGGUCGCGCAGGGCC 20 1498

HSV2-UL19-4326 + CGCGCCAGGUCGCGCAGGGCC 21 8709

HSV2-UL19-4327 + GCGCGCCAGGUCGCGCAGGGCC 22 8710

HSV2-UL19-4328 + CGCGCGCCAGGUCGCGCAGGGCC 23 8711

HSV2-UL19-4329 + UCGCGCGCCAGGUCGCGCAGGGCC 24 8712

HSV2-UL19-4330 + UCCAGGUGCUGGGGGGCC 18 8713

HSV2-UL19-4331 + GUCCAGGUGCUGGGGGGCC 19 8714

HSV2-UL19-2626 + GGUCCAGGUGCUGGGGGGCC 20 2753

HSV2-UL19-4332 + UGGUCCAGGUGCUGGGGGGCC 21 8715 HSV2-UL19-4333 + GUGGUCCAGGUGCUGGGGGGCC 22 8716

HSV2-UL19-4334 + UGUGGUCCAGGUGCUGGGGGGCC 23 8717

HSV2-UL19-4335 + GUGUGGUCCAGGUGCUGGGGGGCC 24

8718

HSV2-UL19-4336 + GGCCACCUGGAGCUGGCC 18 8719

HSV2-UL19-4337 + GGGCCACCUGGAGCUGGCC 19 8720

HSV2-UL19-2575 + CGGGCCACCUGGAGCUGGCC 20 2723

HSV2-UL19-4338 + GCGGGCCACCUGGAGCUGGCC 21 8721

HSV2-UL19-4339 + GGCGGGCCACCUGGAGCUGGCC 22 8722

HSV2-UL19-4340 + UGGCGGGCCACCUGGAGCUGGCC 23 8723

HSV2-UL19-4341 + GUGGCGGGCCACCUGGAGCUGGCC 24 8724

HSV2-UL19-4342 + CACGGGUUGGCCGUGGCC 18 8725

HSV2-UL19-4343 + CCACGGGUUGGCCGUGGCC 19 8726

HSV2-UL19-2536 + CCCACGGGUUGGCCGUGGCC 20 2699

HSV2-UL19-4344 + GCCCACGGGUUGGCCGUGGCC 21 8727

HSV2-UL19-4345 + CGCCCACGGGUUGGCCGUGGCC 22 8728

HSV2-UL19-4346 + ACGCCCACGGGUUGGCCGUGGCC 23 8729

HSV2-UL19-4347 + GACGCCCACGGGUUGGCCGUGGCC 24 8730

HSV2-UL19-4348 + UAGUUGAUGUCCGUGGCC 18 8731

HSV2-UL19-4349 + GUAGUUGAUGUCCGUGGCC 19 8732

HSV2-UL19-2544 + AGUAGUUGAUGUCCGUGGCC 20 2707

HSV2-UL19-4350 + AAGUAGUUGAUGUCCGUGGCC 21 8733

HSV2-UL19-4351 + AAAG UAGUUGAUGUCCGUGGCC 22 8734

HSV2-UL19-4352 + GAAAGUAGUUGAUGUCCGUGGCC 23 8735

HSV2-UL19-4353 + CGAAAGUAGUUGAUGUCCGUGGCC 24 8736

HSV2-UL19-4354 + GCGGGGCUCCGGGUGGCC 18 8737

HSV2-UL19-4355 + CGCGGGGCUCCGGGUGGCC 19 8738

HSV2-UL19-1458 + GCGCGGGGCUCCGGGUGGCC 20 1698

HSV2-UL19-4356 + CGCGCGGGGCUCCGGGUGGCC 21 8739

HSV2-UL19-4357 + ACGCGCGGGGCUCCGGGUGGCC 22 8740

HSV2-UL19-4358 + AACGCGCGGGGCUCCGGGUGGCC 23 8741

HSV2-UL19-4359 + GAACGCGCGGGGCUCCGGGUGGCC 24 8742

HSV2-UL19-4360 + CCCAAAAAAACAGCUGCC 18 8743

HSV2-UL19-4361 + CCCCAAAAAAACAGCUGCC 19 8744

HSV2-UL19-1447 + U CCCCAAAAAAACAG CU G CC 20 1687

HSV2-UL19-4362 + UUCCCCAAAAAAACAGCUGCC 21 8745

HSV2-UL19-4363 + UUUCCCCAAAAAAACAGCUGCC 22 8746

HSV2-UL19-4364 + CUUUCCCCAAAAAAACAGCUGCC 23 8747

HSV2-UL19-4365 + CCUUUCCCCAAAAAAACAGCUGCC 24 8748

HSV2-UL19-4366 + GCGAUGGCCCGCCUUGCC 18 8749

HSV2-UL19-4367 + GGCGAUGGCCCGCCUUGCC 19 8750

HSV2-UL19-2768 + CGGCGAUGGCCCGCCUUGCC 20 2854

HSV2-UL19-4368 + GCGGCGAUGGCCCGCCUUGCC 21 8751

HSV2-UL19-4369 + CGCGGCGAUGGCCCGCCUUGCC 22 8752

HSV2-UL19-4370 + UCGCGGCGAUGGCCCGCCUUGCC 23 8753

HSV2-UL19-4371 + CUCGCGGCGAUGGCCCGCCUUGCC 24 8754 HSV2-UL19-4372 + UACACGGCCAUGCACUCC 18 8755

HSV2-UL19-4373 + AUACACGGCCAUGCACUCC 19 8756

HSV2-UL19-2668 + GAUACACGGCCAUGCACUCC 20 2786

HSV2-UL19-4374 + CGAUACACGGCCAUGCACUCC 21 8757

HSV2-UL19-4375 + CCGAUACACGGCCAUGCACUCC 22 8758

HSV2-UL19-4376 + CCCGAUACACGGCCAUGCACUCC 23 8759

HSV2-UL19-4377 + UCCCGAUACACGGCCAUGCACUCC 24 8760

HSV2-UL19-4378 + CGUCUCUCCAGGGCCUCC 18 8761

HSV2-UL19-4379 + GCGUCUCUCCAGGGCCUCC 19 8762

HSV2-UL19-1486 + UGCGUCUCUCCAGGGCCUCC 20 1726

HSV2-UL19-4380 + AUGCGUCUCUCCAGGGCCUCC 21 8763

HSV2-UL19-4381 + GAUGCGUCUCUCCAGGGCCUCC 22 8764

HSV2-UL19-4382 + AGAUGCGUCUCUCCAGGGCCUCC 23 8765

HSV2-UL19-4383 + UAGAUGCGUCUCUCCAGGGCCUCC 24 8766

HSV2-UL19-4384 + G U CCCG ACCG CG U U CU CC 18 8767

HSV2-UL19-4385 + GGUCCCGACCGCGUUCUCC 19 8768

HSV2-UL19-2722 + CGGUCCCGACCGCGUUCUCC 20 2826

HSV2-UL19-4386 + ACGGUCCCGACCGCGUUCUCC 21 8769

HSV2-UL19-4387 + CACGGUCCCGACCGCGUUCUCC 22 8770

HSV2-UL19-4388 + ACACGGUCCCGACCGCGUUCUCC 23 8771

HSV2-UL19-4389 + CACACGGUCCCGACCGCGUUCUCC 24 8772

HSV2-UL19-4390 + GUCGCCGUGGUCAGGUCC 18 8773

HSV2-UL19-4391 + CGUCGCCGUGGUCAGGUCC 19 8774

HSV2-UL19-2767 + GCGUCGCCGUGGUCAGGUCC 20 2853

HSV2-UL19-4392 + UGCGUCGCCGUGGUCAGGUCC 21 8775

HSV2-UL19-4393 + CUGCGUCGCCGUGGUCAGGUCC 22 8776

HSV2-UL19-4394 + GCUGCGUCGCCGUGGUCAGGUCC 23 8777

HSV2-UL19-4395 + GGCUGCGUCGCCGUGGUCAGGUCC 24 8778

HSV2-UL19-4396 + GCCGGGUAGCUGCGGUCC 18 8779

HSV2-UL19-4397 + CGCCGGGUAGCUGCGGUCC 19 8780

HSV2-UL19-2682 + CCGCCGGGUAGCUGCGGUCC 20 2797

HSV2-UL19-4398 + ACCGCCGGGUAGCUGCGGUCC 21 8781

HSV2-UL19-4399 + CACCGCCGGGUAGCUGCGGUCC 22 8782

HSV2-UL19-4400 + ACACCGCCGGGUAGCUGCGGUCC 23 8783

HSV2-UL19-4401 + AACACCGCCGGGUAGCUGCGGUCC 24 8784

HSV2-UL19-4402 + AGGCCGCACGGGUCU UCC 18 8785

HSV2-UL19-4403 + CAGGCCGCACGGGUCUUCC 19 8786

HSV2-UL19-2519 + ACAGGCCGCACGGGUCUUCC 20 2683

HSV2-UL19-4404 + AACAGGCCGCACGGGUCUUCC 21 8787

HSV2-UL19-4405 + AAACAGGCCGCACGGGUCUUCC 22 8788

HSV2-UL19-4406 + GAAACAGGCCGCACGGGUCUUCC 23 8789

HSV2-UL19-4407 + UGAAACAGGCCGCACGGGUCUUCC 24 8790

HSV2-UL19-4408 + CGGUGAAGAACUUGAAGC 18 8791

HSV2-UL19-4409 + GCGGUGAAGAACUUGAAGC 19 8792

HSV2-UL19-1136 + CGCGGUGAAGAACUUGAAGC 20 1376

HSV2-UL19-4410 + CCGCGGUGAAGAACUUGAAGC 21 8793 HSV2-UL19-4411 + GCCGCGGUGAAGAACUUGAAGC 22 8794

HSV2-UL19-4412 + GGCCGCGGUGAAGAACUUGAAGC 23 8795

HSV2-UL19-4413 + CGGCCGCGGUGAAGAACUUGAAGC 24 8796

HSV2-UL19-4414 + GCCGCGUUGUCUAGCAGC 18 8797

HSV2-UL19-4415 + GGCCGCGUUGUCUAGCAGC 19 8798

HSV2-UL19-1189 + CGGCCGCGUUGUCUAGCAGC 20 1429

HSV2-UL19-4416 + GCGGCCGCGUUGUCUAGCAGC 21 8799

HSV2-UL19-4417 + GGCGGCCGCGUUGUCUAGCAGC 22 8800

HSV2-UL19-4418 + CGGCGGCCGCGUUGUCUAGCAGC 23 8801

HSV2-UL19-4419 + ACGGCGGCCGCGUUGUCUAGCAGC 24 8802

HSV2-UL19-4420 + UGCGCCAGCACCUGCAGC 18 8803

HSV2-UL19-4421 + GUGCGCCAGCACCUGCAGC 19 8804

HSV2-UL19-2631 + UGUGCGCCAGCACCUGCAGC 20 2757

HSV2-UL19-4422 + UUGUGCGCCAGCACCUGCAGC 21 8805

HSV2-UL19-4423 + G U U G U GCG CCAG CACCU GCAG C 22 8806

HSV2-UL19-4424 + UGUUGUGCGCCAGCACCUGCAGC 23 8807

HSV2-UL19-4425 + AUGUUGUGCGCCAGCACCUGCAGC 24 8808

HSV2-UL19-4426 + CCGCGCGGCUGGGUGAGC 18 8809

HSV2-UL19-4427 + UCCGCGCGGCUGGGUGAGC 19 8810

HSV2-UL19-2571 + UUCCGCGCGGCUGGGUGAGC 20 2720

HSV2-UL19-4428 + UU UCCGCGCGGCUGGGUGAGC 21 8811

HSV2-UL19-4429 + GUUUCCGCGCGGCUGGGUGAGC 22 8812

HSV2-UL19-4430 + CGUUUCCGCGCGGCUGGGUGAGC 23 8813

HSV2-UL19-4431 + ACGUUUCCGCGCGGCUGGGUGAGC 24 8814

HSV2-UL19-4432 + AGAGACAGGCCCUUUAGC 18 8815

HSV2-UL19-4433 + CAGAGACAGGCCCUUUAGC 19 8816

HSV2-UL19-1102 + ACAGAGACAGGCCCUUUAGC 20 1342

HSV2-UL19-4434 + UACAGAGACAGGCCCUUUAGC 21 8817

HSV2-UL19-4435 + UUACAGAGACAGGCCCUUUAGC 22 8818

HSV2-UL19-4436 + UUUACAGAGACAGGCCCUUUAGC 23 8819

HSV2-UL19-4437 + UUUUACAGAGACAGGCCCUUUAGC 24 8820

HSV2-UL19-4438 + CUGCCGGGGAGGGAACGC 18 8821

HSV2-UL19-4439 + GCUGCCGGGGAGGGAACGC 19 8822

HSV2-UL19-2731 + AGCUGCCGGGGAGGGAACGC 20 2830

HSV2-UL19-4440 + CAGCUGCCGGGGAGGGAACGC 21 8823

HSV2-UL19-4441 + ACAGCUGCCGGGGAGGGAACGC 22 8824

HSV2-UL19-4442 + AACAGCUGCCGGGGAGGGAACGC 23 8825

HSV2-UL19-4443 + AAACAGCUGCCGGGGAGGGAACGC 24 8826

HSV2-UL19-4444 + GUGCAGCAGCGCCCCCGC 18 8827

HSV2-UL19-4445 + GGUGCAGCAGCGCCCCCGC 19 8828

HSV2-UL19-2652 + CGGUGCAGCAGCGCCCCCGC 20 2771

HSV2-UL19-4446 + GCGGUGCAGCAGCGCCCCCGC 21 8829

HSV2-UL19-4447 + CGCGGUGCAGCAGCGCCCCCGC 22 8830

HSV2-UL19-4448 + CCGCGGUGCAGCAGCGCCCCCGC 23 8831

HSV2-UL19-4449 + CCCGCGGUGCAGCAGCGCCCCCGC 24 8832

HSV2-UL19-4450 + CCGGGGGGACUUCGCCGC 18 8833 HSV2-UL19-4451 + GCCGGGGGGACUUCGCCGC 19 8834

HSV2-UL19-1408 + GGCCGGGGGGACU UCGCCGC 20 1648

HSV2-UL19-4452 + CGGCCGGGGGGACUUCGCCGC 21 8835

HSV2-UL19-4453 + CCGGCCGGGGGGACUUCGCCGC 22 8836

HSV2-UL19-4454 + ACCGGCCGGGGGGACUUCGCCGC 23 8837

HSV2-UL19-4455 + GACCGGCCGGGGGGACU UCGCCGC 24 8838

HSV2-UL19-4456 + GUCGGCGGCGUCGGCCGC 18 8839

HSV2-UL19-4457 + CGUCGGCGGCGUCGGCCGC 19 8840

HSV2-UL19-2654 + UCGUCGGCGGCGUCGGCCGC 20 2773

HSV2-UL19-4458 + GUCGUCGGCGGCGUCGGCCGC 21 8841

HSV2-UL19-4459 + GGUCGUCGGCGGCGUCGGCCGC 22 8842

HSV2-UL19-4460 + CGGUCGUCGGCGGCGUCGGCCGC 23 8843

HSV2-UL19-4461 + CCGGUCGUCGGCGGCGUCGGCCGC 24 8844

HSV2-UL19-4462 + GGCGCCCGCAAACAGCGC 18 8845

HSV2-UL19-4463 + CGGCGCCCGCAAACAGCGC 19 8846

HSV2-UL19-2617 + UCGGCGCCCGCAAACAGCGC 20 2747

HSV2-UL19-4464 + GUCGGCGCCCGCAAACAGCGC 21 8847

HSV2-UL19-4465 + G G U CG G CG CCCG CAAACAG CG C 22 8848

HSV2-UL19-4466 + UGGUCGGCGCCCGCAAACAGCGC 23 8849

HSV2-UL19-4467 + GUGGUCGGCGCCCG CAAACAG CG C 24 8850

HSV2-UL19-4468 + CGCCACCGGACACAGCGC 18 8851

HSV2-UL19-4469 + ACGCCACCGGACACAGCGC 19 8852

HSV2-UL19-2688 + AACGCCACCGGACACAGCGC 20 2803

HSV2-UL19-4470 + AAACG CCACCG G ACACAG CG C 21 8853

HSV2-UL19-4471 + GAAACGCCACCGGACACAGCGC 22 8854

HSV2-UL19-4472 + CGAAACGCCACCGGACACAGCGC 23 8855

HSV2-UL19-4473 + ACGAAACGCCACCGGACACAGCGC 24 8856

HSV2-UL19-4474 + CUGAGUCACGAGCCGCGC 18 8857

HSV2-UL19-4475 + ACUGAGUCACGAGCCGCGC 19 8858

HSV2-UL19-2678 + CACUGAGUCACGAGCCGCGC 20 2793

HSV2-UL19-4476 + GCACUGAGUCACGAGCCGCGC 21 8859

HSV2-UL19-4477 + UGCACUGAGUCACGAGCCGCGC 22 8860

HSV2-UL19-4478 + AU GCACU G AG U CACG AG CCG CGC 23 8861

HSV2-UL19-4479 + GAUGCACUGAGUCACGAGCCGCGC 24 8862

HSV2-UL19-4480 + GCUGCUGCAUGUCCGCGC 18 8863

HSV2-UL19-4481 + CGCUGCUGCAUGUCCGCGC 19 8864

HSV2-UL19-1427 + ACGCUGCUGCAUGUCCGCGC 20 1667

HSV2-UL19-4482 + AACGCUGCUGCAUGUCCGCGC 21 8865

HSV2-UL19-4483 + AAACGCUGCUGCAUGUCCGCGC 22 8866

HSV2-UL19-4484 + AAAACGCUGCUGCAUGUCCGCGC 23 8867

HSV2-UL19-4485 + GAAAACGCUGCUGCAUGUCCGCGC 24 8868

HSV2-UL19-4486 + UACGCCUCCGACGCGCGC 18 8869

HSV2-UL19-4487 + GUACGCCUCCGACGCGCGC 19 8870

HSV2-UL19-2576 + AGUACGCCUCCGACGCGCGC 20 2724

HSV2-UL19-4488 + AAGUACGCCUCCGACGCGCGC 21 8871

HSV2-UL19-4489 + AAAGUACGCCUCCGACGCGCGC 22 8872 HSV2-UL19-4490 + GAAAGUACGCCUCCGACGCGCGC 23 8873

HSV2-UL19-4491 + AGAAAGUACGCCUCCGACGCGCGC 24 8874

HSV2-UL19-4492 + AAAGUGGGUCUCUCGCGC 18 8875

HSV2-UL19-4493 + UAAAGUGGGUCUCUCGCGC 19 8876

HSV2-UL19-2511 + GUAAAGUGGGUCUCUCGCGC 20 2676

HSV2-UL19-4494 + CGUAAAGUGGGUCUCUCGCGC 21 8877

HSV2-UL19-4495 + GCGUAAAGUGGGUCUCUCGCGC 22 8878

HSV2-UL19-4496 + UGCGUAAAGUGGGUCUCUCGCGC 23 8879

HSV2-UL19-4497 + CUGCGUAAAGUGGGUCUCUCGCGC 24 8880

HSV2-UL19-3246 + ACGGCCUGGACGUUGCGC 18 7629

HSV2-UL19-3247 + GACGGCCUGGACGUUGCGC 19 7630

HSV2-UL19-3248 + GGACGGCCUGGACGUUGCGC 20 7631

HSV2-UL19-3249 + AGGACGGCCUGGACGUUGCGC 21 7632

HSV2-UL19-3250 + CAGGACGGCCUGGACGUUGCGC 22 7633

HSV2-UL19-3251 + CCAGGACGGCCUGGACGUUGCGC 23 7634

HSV2-UL19-3252 + CCCAGGACGGCCUGGACGUUGCGC 24 7635

HSV2-UL19-4498 + GGUCGGUCACGGGAUCGC 18 8881

HSV2-UL19-4499 + GGGUCGGUCACGGGAUCGC 19 8882

HSV2-UL19-2643 + GGGGUCGGUCACGGGAUCGC 20 2765

HSV2-UL19-4500 + CGGGGUCGGUCACGGGAUCGC 21 8883

HSV2-UL19-4501 + GCGGGGUCGGUCACGGGAUCGC 22 8884

HSV2-UL19-4502 + GGCGGGGUCGGUCACGGGAUCGC 23 8885

HSV2-UL19-4503 + GGGCGGGGUCGGUCACGGGAUCGC 24 8886

HSV2-UL19-4504 + CUCGGCGACCAGGGUCGC 18 8887

HSV2-UL19-4505 + GCUCGGCGACCAGGGUCGC 19 8888

HSV2-UL19-2772 + AGCUCGGCGACCAGGGUCGC 20 2857

HSV2-UL19-4506 + CAGCUCGGCGACCAGGGUCGC 21 8889

HSV2-UL19-4507 + UCAGCUCGGCGACCAGGGUCGC 22 8890

HSV2-UL19-4508 + UUCAGCUCGGCGACCAGGGUCGC 23 8891

HSV2-UL19-4509 + CUUCAGCUCGGCGACCAGGGUCGC 24 8892

HSV2-UL19-3253 + AAACGCCCCCAGGACGGC 18 7636

HSV2-UL19-3254 + CAAACGCCCCCAGGACGGC 19 7637

HSV2-UL19-2781 + UCAAACGCCCCCAGGACGGC 20 2863

HSV2-UL19-4510 + CUCAAACGCCCCCAGGACGGC 21 8893

HSV2-UL19-4511 + GCUCAAACGCCCCCAGGACGGC 22 8894

HSV2-UL19-4512 + CGCUCAAACGCCCCCAGGACGGC 23 8895

HSV2-UL19-4513 + GCGCUCAAACGCCCCCAGGACGGC 24 8896

HSV2-UL19-4514 + GAUCGCCCCCGGACCGGC 18 8897

HSV2-UL19-4515 + GGAUCGCCCCCGGACCGGC 19 8898

HSV2-UL19-1403 + UGGAUCGCCCCCGGACCGGC 20 1643

HSV2-UL19-4516 + CUGGAUCGCCCCCGGACCGGC 21 8899

HSV2-UL19-4517 + CCUGGAUCGCCCCCGGACCGGC 22 8900

HSV2-UL19-4518 + GCCUGGAUCGCCCCCGGACCGGC 23 8901

HSV2-UL19-4519 + GGCCUGGAUCGCCCCCGGACCGGC 24 8902

HSV2-UL19-4520 + GUCCGCGCCGGGGCCGGC 18 8903

HSV2-UL19-4521 + UGUCCGCGCCGGGGCCGGC 19 8904 HSV2-UL19-1431 + AUGUCCGCGCCGGGGCCGGC 20 1671

HSV2-UL19-4522 + CAUGUCCGCGCCGGGGCCGGC 21 8905

HSV2-UL19-4523 + GCAUGUCCGCGCCGGGGCCGGC 22 8906

HSV2-UL19-4524 + UGCAUGUCCGCGCCGGGGCCGGC 23 8907

HSV2-UL19-4525 + CUGCAUGUCCGCGCCGGGGCCGGC 24 8908

HSV2-UL19-4526 + CAGGUGAUUCAGCUCGGC 18 8909

HSV2-UL19-4527 + UCAGGUGAUUCAGCUCGGC 19 8910

HSV2-UL19-2662 + AUCAGGUGAUUCAGCUCGGC 20 2780

HSV2-UL19-4528 + CAUCAGGUGAUUCAGCUCGGC 21 8911

HSV2-UL19-4529 + GCAUCAGGUGAUUCAGCUCGGC 22 8912

HSV2-UL19-4530 + CGCAUCAGGUGAUUCAGCUCGGC 23 8913

HSV2-UL19-4531 + GCGCAUCAGGUGAUUCAGCUCGGC 24 8914

HSV2-UL19-4532 + GUAGUUGGCCCCCAGGGC 18 8915

HSV2-UL19-4533 + AGUAGUUGGCCCCCAGGGC 19 8916

HSV2-UL19-1245 + AAGUAGUUGGCCCCCAGGGC 20 1485

HSV2-UL19-4534 + GAAGUAGUUGGCCCCCAGGGC 21 8917

HSV2-UL19-4535 + AGAAGUAGUUGGCCCCCAGGGC 22 8918

HSV2-UL19-4536 + GAGAAGUAGUUGGCCCCCAGGGC 23 8919

HSV2-UL19-4537 + GGAGAAGUAGUUGGCCCCCAGGGC 24 8920

HSV2-UL19-4538 + CAU G G G CAACAG C AG G G C 18 8921

HSV2-UL19-4539 + GCAUGGGCAACAGCAGGGC 19 8922

HSV2-UL19-2776 + UGCAUGGGCAACAGCAGGGC 20 2861

HSV2-UL19-4540 + UU GCAUGGGCAACAGCAGGGC 21 8923

HSV2-UL19-4541 + GU UGCAUGGGCAACAGCAGGGC 22 8924

HSV2-UL19-4542 + CGU UGCAUGGGCAACAGCAGGGC 23 8925

HSV2-UL19-4543 + UCGUUGCAUGGGCAACAGCAGGGC 24 8926

HSV2-UL19-4544 + CGCCAGGUCGCGCAGGGC 18 8927

HSV2-UL19-4545 + GCGCCAGGUCGCGCAGGGC 19 8928

HSV2-UL19-1257 + CGCGCCAGGUCGCGCAGGGC 20 1497

HSV2-UL19-4546 + GCGCGCCAGGUCGCGCAGGGC 21 8929

HSV2-UL19-4547 + CGCGCGCCAGGUCGCGCAGGGC 22 8930

HSV2-UL19-4548 + UCGCGCGCCAGGUCGCGCAGGGC 23 8931

HSV2-UL19-4549 + GUCGCGCGCCAGGUCGCGCAGGGC 24 8932

HSV2-UL19-4550 + GUCGUCCACCAGCUGGGC 18 8933

HSV2-UL19-4551 + AGUCGUCCACCAGCUGGGC 19 8934

HSV2-UL19-2666 + AAGUCGUCCACCAGCUGGGC 20 2784

HSV2-UL19-4552 + AAAGUCGUCCACCAGCUGGGC 21 8935

HSV2-UL19-4553 + UAAAGUCGUCCACCAGCUGGGC 22 8936

HSV2-UL19-4554 + GUAAAGUCGUCCACCAGCUGGGC 23 8937

HSV2-UL19-4555 + GGUAAAGUCGUCCACCAGCUGGGC 24 8938

HSV2-UL19-4556 + CGCGGGGCUCCGGGUGGC 18 8939

HSV2-UL19-4557 + GCGCGGGGCUCCGGGUGGC 19 8940

HSV2-UL19-1457 + CGCGCGGGGCUCCGGGUGGC 20 1697

HSV2-UL19-4558 + ACGCGCGGGGCUCCGGGUGGC 21 8941

HSV2-UL19-4559 + AACGCGCGGGGCUCCGGGUGGC 22 8942

HSV2-UL19-4560 + GAACGCGCGGGGCUCCGGGUGGC 23 8943 HSV2-UL19-4561 + GGAACGCGCGGGGCUCCGGGUGGC 24 8944

HSV2-UL19-4562 + ACCUUCAGGAAGGACUGC 18 8945

HSV2-UL19-4563 + CACCUUCAGGAAGGACUGC 19 8946

HSV2-UL19-1507 + CCACCUUCAGGAAGGACUGC 20 1747

HSV2-UL19-4564 + UCCACCUUCAGGAAGGACUGC 21 8947

HSV2-UL19-4565 + CUCCACCUUCAGGAAGGACUGC 22 8948

HSV2-UL19-4566 + CCUCCACCUUCAGGAAGGACUGC 23 8949

HSV2-UL19-4567 + UCCUCCACCUUCAGGAAGGACUGC 24 8950

HSV2-UL19-4568 + CCCCAAAAAAACAGCUGC 18 8951

HSV2-UL19-4569 + U CCCCAAAA AAACAG CU G C 19 8952

HSV2-UL19-1446 + UUCCCCAAAAAAACAGCUGC 20 1686

HSV2-UL19-4570 + UU UCCCCAAAAAAACAGCUGC 21 8953

HSV2-UL19-4571 + CUUUCCCCAAAAAAACAGCUGC 22 8954

HSV2-UL19-4572 + CCUUUCCCCAAAAAAACAGCUGC 23 8955

HSV2-UL19-4573 + U CCU U U CCCCAAAAAAACAGCU G C 24 8956

HSV2-UL19-4574 + UUGGCGUUGUCGGGCUGC 18 8957

HSV2-UL19-4575 + GUUGGCGUUGUCGGGCUGC 19 8958

HSV2-UL19-2707 + GGUUGGCGUUGUCGGGCUGC 20 2814

HSV2-UL19-4576 + AGGU UGGCGUUGUCGGGCUGC 21 8959

HSV2-UL19-4577 + CAGGU UGGCGUUGUCGGGCUGC 22 8960

HSV2-UL19-4578 + CCAGGUUGGCGUUGUCGGGCUGC 23 8961

HSV2-UL19-4579 + GCCAGGUUGGCGUUGUCGGGCUGC 24 8962

HSV2-UL19-4580 + AUGGUGUGGUCCAGGUGC 18 8963

HSV2-UL19-4581 + GAUGGUGUGGUCCAGGUGC 19 8964

HSV2-UL19-1275 + GGAUGGUGUGGUCCAGGUGC 20 1515

HSV2-UL19-4582 + UGGAUGGUGUGGUCCAGGUGC 21 8965

HSV2-UL19-4583 + UUGGAUGGUGUGGUCCAGGUGC 22 8966

HSV2-UL19-4584 + UU UGGAUGGUGUGGUCCAGGUGC 23 8967

HSV2-UL19-4585 + UU UUGGAUGGUGUGGUCCAGGUGC 24

8968

HSV2-UL19-4586 + UUGUCGAGAUAUCGUUGC 18 8969

HSV2-UL19-4587 + GUUGUCGAGAUAUCGUUGC 19 8970

HSV2-UL19-2774 + CGUUGUCGAGAUAUCGUUGC 20 2859

HSV2-UL19-4588 + CCGUUGUCGAGAUAUCGUUGC 21 8971

HSV2-UL19-4589 + CCCGUUGUCGAGAUAUCGUUGC 22 8972

HSV2-UL19-4590 + GCCCGUUGUCGAGAUAUCGUUGC 23 8973

HSV2-UL19-4591 + CGCCCGUUGUCGAGAUAUCGUUGC 24 8974

HSV2-UL19-4592 + CGCGUGGGCCUCCCCAUC 18 8975

HSV2-UL19-4593 + GCGCGUGGGCCUCCCCAUC 19 8976

HSV2-UL19-2512 + CGCGCGUGGGCCUCCCCAUC 20 2677

HSV2-UL19-4594 + UCGCGCGUGGGCCUCCCCAUC 21 8977

HSV2-UL19-4595 + CUCGCGCGUGGGCCUCCCCAUC 22 8978

HSV2-UL19-4596 + UCUCGCGCGUGGGCCUCCCCAUC 23 8979

HSV2-UL19-4597 + CUCUCGCGCGUGGGCCUCCCCAUC 24 8980

HSV2-UL19-4598 + AGGGCCACGGGGCUCAUC 18 8981

HSV2-UL19-4599 + CAGGGCCACGGGGCUCAUC 19 8982 HSV2-UL19-2589 + ACAG GGCCACG G GG CU CAU C 20 2733

HSV2-UL19-4600 + UACAGGGCCACGGGGCUCAUC 21 8983

HSV2-UL19-4601 + AUACAGGGCCACGGGGCUCAUC 22 8984

HSV2-UL19-4602 + GAUACAGGGCCACGGGGCUCAUC 23 8985

HSV2-UL19-4603 + UGAUACAGGGCCACGGGGCUCAUC 24 8986

HSV2-UL19-4604 + GCGUCUCUCCAGGGCCUC 18 8987

HSV2-UL19-4605 + UGCGUCUCUCCAGGGCCUC 19 8988

HSV2-UL19-2750 + AUGCGUCUCUCCAGGGCCUC 20 2843

HSV2-UL19-4606 + GAUGCGUCUCUCCAGGGCCUC 21 8989

HSV2-UL19-4607 + AGAUGCGUCUCUCCAGGGCCUC 22 8990

HSV2-UL19-4608 + UAGAUGCGUCUCUCCAGGGCCUC 23 8991

HSV2-UL19-4609 + GUAGAUGCGUCUCUCCAGGGCCUC 24 8992

HSV2-UL19-4610 + CACGGCCAUGCACUCCUC 18 8993

HSV2-UL19-4611 + ACACGGCCAUGCACUCCUC 19 8994

HSV2-UL19-1369 + UACACGGCCAUGCACUCCUC 20 1609

HSV2-UL19-4612 + AUACACGGCCAUGCACUCCUC 21 8995

HSV2-UL19-4613 + GAUACACGGCCAUGCACUCCUC 22 8996

HSV2-UL19-4614 + CGAUACACGGCCAUGCACUCCUC 23 8997

HSV2-UL19-4615 + CCGAUACACGGCCAUGCACUCCUC 24 8998

HSV2-UL19-4616 + GAACGCGGGGUGCAGCUC 18 8999

HSV2-UL19-4617 + CGAACGCGGGGUGCAGCUC 19 9000

HSV2-UL19-2705 + UCGAACGCGGGGUGCAGCUC 20 2812

HSV2-UL19-4618 + GUCGAACGCGGGGUGCAGCUC 21 9001

HSV2-UL19-4619 + AGUCGAACGCGGGGUGCAGCUC 22 9002

HSV2-UL19-4620 + AAGUCGAACGCGGGGUGCAGCUC 23 9003

HSV2-UL19-4621 + GAAGUCGAACGCGGGGUGCAGCUC 24 9004

HSV2-UL19-4622 + CUCACCGGGGGCGAUCUC 18 9005

HSV2-UL19-4623 + CCUCACCGGGGGCGAUCUC 19 9006

HSV2-UL19-1312 + UCCUCACCGGGGGCGAUCUC 20 1552

HSV2-UL19-4624 + CUCCUCACCGGGGGCGAUCUC 21 9007

HSV2-UL19-4625 + ACUCCUCACCGGGGGCGAUCUC 22 9008

HSV2-UL19-4626 + CACUCCUCACCGGGGGCGAUCUC 23 9009

HSV2-UL19-4627 + GCACUCCUCACCGGGGGCGAUCUC 24 9010

HSV2-UL19-4628 + CUCCCCCGCCGCGCUCUC 18 9011

HSV2-UL19-4629 + UCUCCCCCGCCGCGCUCUC 19 9012

HSV2-UL19-2591 + UUCUCCCCCGCCGCGCUCUC 20 2735

HSV2-UL19-4630 + GUUCUCCCCCGCCGCGCUCUC 21 9013

HSV2-UL19-4631 + CGUUCUCCCCCGCCGCGCUCUC 22 9014

HSV2-UL19-4632 + GCGUUCUCCCCCGCCGCGCUCUC 23 9015

HSV2-UL19-4633 + CGCGUUCUCCCCCGCCGCGCUCUC 24 9016

HSV2-UL19-4634 + GGCGUAGAUGCGUCUCUC 18 9017

HSV2-UL19-4635 + CGGCGUAGAUGCGUCUCUC 19 9018

HSV2-UL19-2749 + GCGGCGUAGAUGCGUCUCUC 20 2842

HSV2-UL19-4636 + GGCGGCGUAGAUGCGUCUCUC 21 9019

HSV2-UL19-4637 + UGGCGGCGUAGAUGCGUCUCUC 22 9020

HSV2-UL19-4638 + GUGGCGGCGUAGAUGCGUCUCUC 23 9021 HSV2-UL19-4639 + GGUGGCGGCGUAGAUGCGUCUCUC 24 9022

HSV2-UL19-4640 + CUGGUGAUGCACUGAGUC 18 9023

HSV2-UL19-4641 + GCUGGUGAUGCACUGAGUC 19 9024

HSV2-UL19-2677 + AGCUGGUGAUGCACUGAGUC 20 2792

HSV2-UL19-4642 + UAGCUGGUGAUGCACUGAGUC 21 9025

HSV2-UL19-4643 + GU AGCUGGUGAUGCACUGAGUC 22 9026

HSV2-UL19-4644 + AG UAGCUGGUGAUGCACUGAGUC 23 9027

HSV2-UL19-4645 + CAGUAGCUGGUGAUGCACUGAGUC 24 9028

HSV2-UL19-4646 + CCGGGGUGGAGGCCCGUC 18 9029

HSV2-UL19-4647 + CCCGGGGUGGAGGCCCGUC 19 9030

HSV2-UL19-2585 + ACCCGGGGUGGAGGCCCGUC 20 2730

HSV2-UL19-4648 + AACCCGGGGUGGAGGCCCGUC 21 9031

HSV2-UL19-4649 + GAACCCGGGGUGGAGGCCCGUC 22 9032

HSV2-UL19-4650 + CG AACCCGGGGUGGAGGCCCGUC 23 9033

HSV2-UL19-4651 + CCG AACCCGGGGUGGAGGCCCGUC 24 9034

HSV2-UL19-4652 + CUCCAGGCCCCGGGCGUC 18 9035

HSV2-UL19-4653 + GCUCCAGGCCCCGGGCGUC 19 9036

HSV2-UL19-2686 + AGCUCCAGGCCCCGGGCGUC 20 2801

HSV2-UL19-4654 + GAGCUCCAGGCCCCGGGCGUC 21 9037

HSV2-UL19-4655 + CGAGCUCCAGGCCCCGGGCGUC 22 9038

HSV2-UL19-4656 + CCGAGCUCCAGGCCCCGGGCGUC 23 9039

HSV2-UL19-4657 + GCCGAGCUCCAGGCCCCGGGCGUC 24 9040

HSV2-UL19-4658 + GCAGUCUCGGUGGCGGUC 18 9041

HSV2-UL19-4659 + GGCAGUCUCGGUGGCGGUC 19 9042

HSV2-UL19-2657 + CGGCAGUCUCGGUGGCGGUC 20 2776

HSV2-UL19-4660 + CCGGCAGUCUCGGUGGCGGUC 21 9043

HSV2-UL19-4661 + UCCGGCAGUCUCGGUGGCGGUC 22 9044

HSV2-UL19-4662 + AUCCGGCAGUCUCGGUGGCGGUC 23 9045

HSV2-UL19-4663 + AAUCCGGCAGUCUCGGUGGCGGUC 24 9046

HSV2-UL19-4664 + AACAGCACGUUCUCGGUC 18 9047

HSV2-UL19-4665 + AAACAGCACGUUCUCGGUC 19 9048

HSV2-UL19-2578 + AAAACAGCACGUUCUCGGUC 20 2725

HSV2-UL19-4666 + G AAAACAG CACGUUCUCGGUC 21 9049

HSV2-UL19-4667 + G G AAAACAG CACGUUCUCGGUC 22 9050

HSV2-UL19-4668 + CG G AAAACAG CACGUUCUCGGUC 23 9051

HSV2-UL19-4669 + UCGGAAAACAGCACGUUCUCGGUC 24 9052

HSV2-UL19-4670 + GGGUGGGCGGGGUCGGUC 18 9053

HSV2-UL19-4671 + CGGGUGGGCGGGGUCGGUC 19 9054

HSV2-UL19-2641 + GCGGGUGGGCGGGGUCGGUC 20 2764

HSV2-UL19-4672 + AGCGGGUGGGCGGGGUCGGUC 21 9055

HSV2-UL19-4673 + CAGCGGGUGGGCGGGGUCGGUC 22 9056

HSV2-UL19-4674 + GCAGCGGGUGGGCGGGGUCGGUC 23 9057

HSV2-UL19-4675 + UGCAGCGGGUGGGCGGGGUCGGUC 24

9058

HSV2-UL19-4676 + GCGCGCACGCGCGUUGUC 18 9059

HSV2-UL19-4677 + CGCGCGCACGCGCGUUGUC 19 9060 HSV2-UL19-1488 + CCGCGCGCACGCGCGUUGUC 20 1728

HSV2-UL19-4678 + UCCGCGCGCACGCGCGUUGUC 21 9061

HSV2-UL19-4679 + AUCCGCGCGCACGCGCGUUGUC 22 9062

HSV2-UL19-4680 + GAUCCGCGCGCACGCGCGUUGUC 23 9063

HSV2-UL19-4681 + AGAUCCGCGCGCACGCGCGUUGUC 24 9064

HSV2-UL19-4682 + UCGGUGUCCUCCACCU UC 18 9065

HSV2-UL19-4683 + CUCGGUGUCCUCCACCUUC 19 9066

HSV2-UL19-1505 + CCU CG G U G U CCU CCACCU U C 20 1745

HSV2-UL19-4684 + GCCUCGGUGUCCUCCACCUUC 21 9067

HSV2-UL19-4685 + GGCCUCGGUGUCCUCCACCUUC 22 9068

HSV2-UL19-4686 + CGGCCUCGGUGUCCUCCACCUUC 23 9069

HSV2-UL19-4687 + UCGGCCUCGGUGUCCUCCACCUUC 24 9070

HSV2-UL19-4688 + GGUGAUCGGGUAGGCUUC 18 9071

HSV2-UL19-4689 + AGGUGAUCGGGUAGGCUUC 19 9072

HSV2-UL19-2517 + CAGGUGAUCGGGUAGGCUUC 20 2681

HSV2-UL19-4690 + GCAGGUGAUCGGGUAGGCUUC 21 9073

HSV2-UL19-4691 + CGCAGGUGAUCGGGUAGGCUUC 22 9074

HSV2-UL19-4692 + GCGCAGGUGAUCGGGUAGGCUUC 23 9075

HSV2-UL19-4693 + GGCGCAGGUGAUCGGGUAGGCUUC 24 9076

HSV2-UL19-4694 + ACCAGCGAGUAGUCGUUC 18 9077

HSV2-UL19-4695 + GACCAGCGAGUAGUCGUUC 19 9078

HSV2-UL19-2675 + AGACCAGCGAGUAGUCGUUC 20 2790

HSV2-UL19-4696 + GAG ACCAGCGAGUAGUCGUUC 21 9079

HSV2-UL19-4697 + CGAGACCAGCGAGUAGUCGUUC 22 9080

HSV2-UL19-4698 + ACGAGACCAGCGAGUAGUCGUUC 23 9081

HSV2-UL19-4699 + UACGAGACCAGCGAGUAGUCGUUC 24 9082

HSV2-UL19-4700 + ACGGCCGCGUCGAUGUUC 18 9083

HSV2-UL19-4701 + GACGGCCGCGUCGAUGUUC 19 9084

HSV2-UL19-2719 + CGACGGCCGCGUCGAUGUUC 20 2823

HSV2-UL19-4702 + CCGACGGCCGCGUCGAUGUUC 21 9085

HSV2-UL19-4703 + CCCGACGGCCGCGUCGAUGUUC 22 9086

HSV2-UL19-4704 + CCCCGACGGCCGCGUCGAUGUUC 23 9087

HSV2-UL19-4705 + CCCCCGACGGCCGCGUCGAUGUUC 24 9088

HSV2-UL19-4706 + GCCACGCCCGCGAAGAAG 18 9089

HSV2-UL19-4707 + GGCCACGCCCGCGAAGAAG 19 9090

HSV2-UL19-2702 + CGGCCACGCCCGCGAAGAAG 20 2810

HSV2-UL19-4708 + UCGGCCACGCCCGCGAAGAAG 21 9091

HSV2-UL19-4709 + GUCGGCCACGCCCGCGAAGAAG 22 9092

HSV2-UL19-4710 + CGUCGGCCACGCCCGCGAAGAAG 23 9093

HSV2-UL19-4711 + ACGUCGGCCACGCCCGCGAAGAAG 24 9094

HSV2-UL19-4712 + CGCAGGGCCGGGGGGAAG 18 9095

HSV2-UL19-4713 + GCGCAGGGCCGGGGGGAAG 19 9096

HSV2-UL19-2616 + CGCGCAGGGCCGGGGGGAAG 20 2746

HSV2-UL19-4714 + UCGCGCAGGGCCGGGGGGAAG 21 9097

HSV2-UL19-4715 + GUCGCGCAGGGCCGGGGGGAAG 22 9098

HSV2-UL19-4716 + GGUCGCGCAGGGCCGGGGGGAAG 23 9099 HSV2-UL19-4717 + AGGUCGCGCAGGGCCGGGGGGAAG 24 9100

HSV2-UL19-4718 + GCGGUGAAGAACUUGAAG 18 9101

HSV2-UL19-4719 + CGCGGUGAAGAACUUGAAG 19 9102

HSV2-UL19-2526 + CCGCGGUGAAGAACUUGAAG 20 2690

HSV2-UL19-4720 + GCCGCGGUGAAGAACUUGAAG 21 9103

HSV2-UL19-4721 + GGCCGCGGUGAAGAACUUGAAG 22 9104

HSV2-UL19-4722 + CGGCCGCGGUGAAGAACUUGAAG 23 9105

HSV2-UL19-4723 + UCGGCCGCGGUGAAGAACUUGAAG 24 9106

HSV2-UL19-4724 + UCGUUGCAUGGGCAACAG 18 9107

HSV2-UL19-4725 + AUCGUUGCAUGGGCAACAG 19 9108

HSV2-UL19-2775 + UAUCGUUGCAUGGGCAACAG 20 2860

HSV2-UL19-4726 + AU AUCGUUGCAUGGGCAACAG 21 9109

HSV2-UL19-4727 + GAU AUCGUUGCAUGGGCAACAG 22 9110

HSV2-UL19-4728 + AGAU AUCGUUGCAUGGGCAACAG 23 9111

HSV2-UL19-4729 + GAGAUAUCGUUGCAUGGGCAACAG 24 9112

HSV2-UL19-4730 + GGUAAAGUCGUCCACCAG 18 9113

HSV2-UL19-4731 + G G G U AAAG U CG U CCACCAG 19 9114

HSV2-UL19-2665 + AGGGUAAAGUCGUCCACCAG 20 2783

HSV2-UL19-4732 + CAG G G U AAAG U CG U CCACCAG 21 9115

HSV2-UL19-4733 + GCAGGGUAAAGUCGUCCACCAG 22 9116

HSV2-UL19-4734 + G G CAG G G U AAAG UCGUCCACCAG 23 9117

HSV2-UL19-4735 + CGGCAGGGUAAAGUCGUCCACCAG 24 9118

HSV2-UL19-4736 + CAGGGCCGGGGGGACCAG 18 9119

HSV2-UL19-4737 + CCAGGGCCGGGGGGACCAG 19 9120

HSV2-UL19-1252 + CCCAGGGCCGGGGGGACCAG 20 1492

HSV2-UL19-4738 + CCCCAGGGCCGGGGGGACCAG 21 9121

HSV2-UL19-4739 + CCCCCAGGGCCGGGGGGACCAG 22 9122

HSV2-UL19-4740 + GCCCCCAGGGCCGGGGGGACCAG 23 9123

HSV2-UL19-4741 + GGCCCCCAGGGCCGGGGGGACCAG 24 9124

HSV2-UL19-4742 + G G G CAACAG CAG G G CCAG 18 9125

HSV2-UL19-4743 + U G G G CAACAG CAG G G CCAG 19 9126

HSV2-UL19-1542 + AUGGGCAACAGCAGGGCCAG 20 1782

HSV2-UL19-4744 + CAU G G G CAACAG CAG G G CCAG 21 9127

HSV2-UL19-4745 + GCAUGGGCAACAGCAGGGCCAG 22 9128

HSV2-UL19-4746 + U G CAU G G G CAACAG CAG G G CCAG 23 9129

HSV2-UL19-4747 + UUGCAUGGGCAACAGCAGGGCCAG 24 9130

HSV2-UL19-4748 + GGCCGCGUUGUCUAGCAG 18 9131

HSV2-UL19-4749 + CGGCCGCGUUGUCUAGCAG 19 9132

HSV2-UL19-1188 + GCGGCCGCGUUGUCUAGCAG 20 1428

HSV2-UL19-4750 + GGCGGCCGCGUUGUCUAGCAG 21 9133

HSV2-UL19-4751 + CGGCGGCCGCGUUGUCUAGCAG 22 9134

HSV2-UL19-4752 + ACGGCGGCCGCGUUGUCUAGCAG 23 9135

HSV2-UL19-4753 + CACGGCGGCCGCGUUGUCUAGCAG 24 9136

HSV2-UL19-4754 + CGUGUCGGCGUGCGUCAG 18 9137

HSV2-UL19-4755 + GCGUGUCGGCGUGCGUCAG 19 9138

HSV2-UL19-2764 + CGCGUGUCGGCGUGCGUCAG 20 2851 HSV2-UL19-4756 + GCGCGUGUCGGCGUGCGUCAG 21 9139

HSV2-UL19-4757 + CGCGCGUGUCGGCGUGCGUCAG 22 9140

HSV2-UL19-4758 + CCGCGCGUGUCGGCGUGCGUCAG 23 9141

HSV2-UL19-4759 + CCCGCGCGUGUCGGCGUGCGUCAG 24 9142

HSV2-UL19-4760 + ACGGGCUGGCGGAUGGAG 18 9143

HSV2-UL19-4761 + CACGGGCUGGCGGAUGGAG 19 9144

HSV2-UL19-2598 + CCACGGGCUGGCGGAUGGAG 20 2740

HSV2-UL19-4762 + ACCACGGGCUGGCGGAUGGAG 21 9145

HSV2-UL19-4763 + CACCACGGGCUGGCGGAUGGAG 22 9146

HSV2-UL19-4764 + GCACCACGGGCUGGCGGAUGGAG 23 9147

HSV2-UL19-4765 + UGCACCACGGGCUGGCGGAUGGAG 24 9148

HSV2-UL19-4766 + UUGCACGCCGCCGCGUAG 18 9149

HSV2-UL19-4767 + GUUGCACGCCGCCGCGUAG 19 9150

HSV2-UL19-2656 + CGUUGCACGCCGCCGCGUAG 20 2775

HSV2-UL19-4768 + ACG UU GCACGCCGCCGCG UAG 21 9151

HSV2-UL19-4769 + CACGUUGCACGCCGCCGCGUAG 22 9152

HSV2-UL19-4770 + CCACGUUGCACGCCGCCGCGUAG 23 9153

HSV2-UL19-4771 + GCCACGUUGCACGCCGCCGCGUAG 24 9154

HSV2-UL19-4772 + AUCGCGGGCGCUGCGUAG 18 9155

HSV2-UL19-4773 + CAUCGCGGGCGCUGCGUAG 19 9156

HSV2-UL19-2513 + CCAUCGCGGGCGCUGCGUAG 20 2678

HSV2-UL19-4774 + CCCAUCGCGGGCGCUGCGUAG 21 9157

HSV2-UL19-4775 + CCCCAUCGCGGGCGCUGCGUAG 22 9158

HSV2-UL19-4776 + UCCCCAUCGCGGGCGCUGCGUAG 23 9159

HSV2-UL19-4777 + CUCCCCAUCGCGGGCGCUGCGUAG 24 9160

HSV2-UL19-4778 + AGCGGGGAGGCGUCGUAG 18 9161

HSV2-UL19-4779 + UAGCGGGGAGGCGUCGUAG 19 9162

HSV2-UL19-2509 + UU AGCGGGGAGGCGUCGUAG 20 2674

HSV2-UL19-4780 + UUUAGCGGGGAGGCGUCGUAG 21 9163

HSV2-UL19-4781 + CUU UAGCGGGGAGGCGUCGUAG 22 9164

HSV2-UL19-4782 + CCU U UAGCGGGGAGGCGUCGUAG 23 9165

HSV2-UL19-4783 + CCCUUUAGCGGGGAGGCGUCGUAG 24 9166

HSV2-UL19-4784 + CAGAGACAGGCCCUUUAG 18 9167

HSV2-UL19-4785 + ACAGAGACAGGCCCUUUAG 19 9168

HSV2-UL19-1101 + UACAGAGACAGGCCCUUUAG 20 1341

HSV2-UL19-4786 + UU ACAGAGACAGGCCCUUUAG 21 9169

HSV2-UL19-4787 + UUUACAGAGACAGGCCCUUUAG 22 9170

HSV2-UL19-4788 + UU UUACAGAGACAGGCCCUUUAG 23 9171

HSV2-UL19-4789 + AU U U UACAG AGACAGGCCCU UU AG 24 9172

HSV2-UL19-4790 + AUGGCGUGGCGGCCAACG 18 9173

HSV2-UL19-4791 + CAUGGCGUGGCGG CCAACG 19 9174

HSV2-UL19-2684 + CCAUGGCGUGGCGGCCAACG 20 2799

HSV2-UL19-4792 + GCCAUGGCGUGGCGGCCAACG 21 9175

HSV2-UL19-4793 + CGCCAUGGCGUGGCGGCCAACG 22 9176

HSV2-UL19-4794 + GCGCCAUGGCGUGGCGGCCAACG 23 9177

HSV2-UL19-4795 + GGCGCCAUGGCGUGGCGGCCAACG 24 9178 HSV2-UL19-4796 + CCGUCGCAGUCCCACACG 18 9179

HSV2-UL19-4797 + GCCGUCGCAGUCCCACACG 19 9180

HSV2-UL19-1347 + GGCCGUCGCAGUCCCACACG 20 1587

HSV2-UL19-4798 + AGGCCGUCGCAGUCCCACACG 21 9181

HSV2-UL19-4799 + AAGGCCGUCGCAGUCCCACACG 22 9182

HSV2-UL19-4800 + UAAGGCCGUCGCAGUCCCACACG 23 9183

HSV2-UL19-4801 + AUAAGGCCGUCGCAGUCCCACACG 24 9184

HSV2-UL19-4802 + GCCGAUCCCGUUUCCACG 18 9185

HSV2-UL19-4803 + CGCCGAUCCCGUUUCCACG 19 9186

HSV2-UL19-2522 + CCGCCG AUCCCGU U UCCACG 20 2686

HSV2-UL19-4804 + ACCGCCGAUCCCGUUUCCACG 21 9187

HSV2-UL19-4805 + UACCGCCGAUCCCGUUUCCACG 22 9188

HSV2-UL19-4806 + AUACCGCCGAUCCCGU UUCCACG 23 9189

HSV2-UL19-4807 + GAUACCGCCGAUCCCGUUUCCACG 24 9190

HSV2-UL19-4808 + CGGUCCUGCCGCACGACG 18 9191

HSV2-UL19-4809 + GCGGUCCUGCCGCACGACG 19 9192

HSV2-UL19-2579 + AGCGGUCCUGCCGCACGACG 20 2726

HSV2-UL19-4810 + AAGCGGUCCUGCCGCACGACG 21 9193

HSV2-UL19-4811 + GAAGCGGUCCUGCCGCACGACG 22 9194

HSV2-UL19-4812 + CGAAG CG G U CCU GCCGCACG ACG 23 9195

HSV2-UL19-4813 + ACGAAGCGGUCCUGCCGCACGACG 24 9196

HSV2-UL19-4814 + GCGUGGACGGGCAGGACG 18 9197

HSV2-UL19-4815 + CGCGUGGACGGGCAGGACG 19 9198

HSV2-UL19-2620 + GCGCGUGGACGGGCAGGACG 20 2750

HSV2-UL19-4816 + AGCGCGUGGACGGGCAGGACG 21 9199

HSV2-UL19-4817 + CAGCGCGUGGACGGGCAGGACG 22 9200

HSV2-UL19-4818 + ACAGCGCGUGGACGGGCAGGACG 23 9201

HSV2-UL19-4819 + AACAGCGCGUGGACGGGCAGGACG 24 9202

HSV2-UL19-4820 + CGAGCUCGCGGCACCCCG 18 9203

HSV2-UL19-4821 + ACGAGCUCGCGGCACCCCG 19 9204

HSV2-UL19-2520 + CACGAGCUCGCGGCACCCCG 20 2684

HSV2-UL19-4822 + CCACGAGCUCGCGGCACCCCG 21 9205

HSV2-UL19-4823 + UCCACGAGCUCGCGGCACCCCG 22 9206

HSV2-UL19-4824 + UUCCACGAGCUCGCGGCACCCCG 23 9207

HSV2-UL19-4825 + CUUCCACGAGCUCGCGGCACCCCG 24 9208

HSV2-UL19-4826 + CCGGCGUGCAGCGCCCCG 18 9209

HSV2-UL19-4827 + GCCGGCGUGCAGCGCCCCG 19 9210

HSV2-UL19-2627 + CGCCGGCGUGCAGCGCCCCG 20 2754

HSV2-UL19-4828 + ACGCCGGCGUGCAGCGCCCCG 21 9211

HSV2-UL19-4829 + CACGCCGGCGUGCAGCGCCCCG 22 9212

HSV2-UL19-4830 + GCACGCCGGCGUGCAGCGCCCCG 23 9213

HSV2-UL19-4831 + AGCACGCCGGCGUGCAGCGCCCCG 24 9214

HSV2-UL19-4832 + AGGACGGGCGAGGCCCCG 18 9215

HSV2-UL19-4833 + GAGGACGGGCGAGGCCCCG 19 9216

HSV2-UL19-2532 + UGAGGACGGGCGAGGCCCCG 20 2695

HSV2-UL19-4834 + CUGAGGACGGGCGAGGCCCCG 21 9217 HSV2-UL19-4835 + GCUGAGGACGGGCGAGGCCCCG 22 9218

HSV2-UL19-4836 + GGCUGAGGACGGGCGAGGCCCCG 23 9219

HSV2-UL19-4837 + GGGCUGAGGACGGGCGAGGCCCCG 24 9220

HSV2-UL19-4838 + GUUCUCCAUGGACAGCCG 18 9221

HSV2-UL19-4839 + CGUUCUCCAUGGACAGCCG 19 9222

HSV2-UL19-2723 + GCGUUCUCCAUGGACAGCCG 20 2827

HSV2-UL19-4840 + CG CG U U CU CCAU G G ACAGCCG 21 9223

HSV2-UL19-4841 + CCGCGUUCUCCAUGGACAGCCG 22 9224

HSV2-UL19-4842 + ACCG CG U U CU CCAU G G ACAG CCG 23 9225

HSV2-UL19-4843 + GACCGCGUUCUCCAUGGACAGCCG 24 9226

HSV2-UL19-4844 + CGCCCGCGUCGGGCGCCG 18 9227

HSV2-UL19-4845 + GCGCCCGCGUCGGGCGCCG 19 9228

HSV2-UL19-1286 + GGCGCCCGCGUCGGGCGCCG 20 1526

HSV2-UL19-4846 + UGGCGCCCGCGUCGGGCGCCG 21 9229

HSV2-UL19-4847 + UUGGCGCCCGCGUCGGGCGCCG 22 9230

HSV2-UL19-4848 + GUUGGCGCCCGCGUCGGGCGCCG 23 9231

HSV2-UL19-4849 + UGU UGGCGCCCGCGUCGGGCGCCG 24 9232

HSV2-UL19-4850 + GCCGGGGGGACUUCGCCG 18 9233

HSV2-UL19-4851 + GGCCGGGGGGACUUCGCCG 19 9234

HSV2-UL19-2697 + CGGCCGGGGGGACU UCGCCG 20 2807

HSV2-UL19-4852 + CCGGCCGGGGGGACUUCGCCG 21 9235

HSV2-UL19-4853 + ACCGGCCGGGGGGACU UCGCCG 22 9236

HSV2-UL19-4854 + GACCGGCCGGGGGGACUUCGCCG 23 9237

HSV2-UL19-4855 + GGACCGGCCGGGGGGACUUCGCCG 24 9238

HSV2-UL19-4856 + UCGCCCCCGGACCGGCCG 18 9239

HSV2-UL19-4857 + AUCGCCCCCGGACCGGCCG 19 9240

HSV2-UL19-1405 + GAUCGCCCCCGGACCGGCCG 20 1645

HSV2-UL19-4858 + GGAUCGCCCCCGGACCGGCCG 21 9241

HSV2-UL19-4859 + UGGAUCGCCCCCGGACCGGCCG 22 9242

HSV2-UL19-4860 + CUGGAUCGCCCCCGGACCGGCCG 23 9243

HSV2-UL19-4861 + CCUGGAUCGCCCCCGGACCGGCCG 24 9244

HSV2-UL19-4862 + AGUUGGCCCCCAGGGCCG 18 9245

HSV2-UL19-4863 + UAGUUGGCCCCCAGGGCCG 19 9246

HSV2-UL19-1247 + GUAGUUGGCCCCCAGGGCCG 20 1487

HSV2-UL19-4864 + AG UAGUUGGCCCCCAGGGCCG 21 9247

HSV2-UL19-4865 + AAGU AGUUGGCCCCCAGGGCCG 22 9248

HSV2-UL19-4866 + GAAGU AGUUGGCCCCCAGGGCCG 23 9249

HSV2-UL19-4867 + AGAAG UAG U UGGCCCCCAGGGCCG 24 9250

HSV2-UL19-4868 + CCAGGUCGCGCAGGGCCG 18 9251

HSV2-UL19-4869 + GCCAGGUCGCGCAGGGCCG 19 9252

HSV2-UL19-1259 + CGCCAGGUCGCGCAGGGCCG 20 1499

HSV2-UL19-4870 + GCGCCAGGUCGCGCAGGGCCG 21 9253

HSV2-UL19-4871 + CGCGCCAGGUCGCGCAGGGCCG 22 9254

HSV2-UL19-4872 + GCGCGCCAGGUCGCGCAGGGCCG 23 9255

HSV2-UL19-4873 + CGCGCGCCAGGUCGCGCAGGGCCG 24 9256

HSV2-UL19-4874 + CCGCGGGUUGCAGGGCCG 18 9257 HSV2-UL19-4875 + CCCGCGGGUUGCAGGGCCG 19 9258

HSV2-UL19-2543 + CCCCGCGGGUUGCAGGGCCG 20 2706

HSV2-UL19-4876 + UCCCCGCGGGUUGCAGGGCCG 21 9259

HSV2-UL19-4877 + GUCCCCGCGGGUUGCAGGGCCG 22 9260

HSV2-UL19-4878 + CGUCCCCGCGGGUUGCAGGGCCG 23 9261

HSV2-UL19-4879 + GCGUCCCCGCGGGUUGCAGGGCCG 24 9262

HSV2-UL19-4880 + CCAAAA AAACAG CU G CCG 18 9263

HSV2-UL19-4881 + CCCAAAAAAACAG C U G CCG 19 9264

HSV2-UL19-1448 + CCCCAAAAAAACAGCUGCCG 20 1688

HSV2-UL19-4882 + UCCCCAAAAAAACAGCUGCCG 21 9265

HSV2-UL19-4883 + UUCCCCAAAAAAACAGCUGCCG 22 9266

HSV2-UL19-4884 + UU UCCCCAAAAAAACAGCUGCCG 23 9267

HSV2-UL19-4885 + CUUUCCCCAAAAAAACAGCUGCCG 24 9268

HSV2-UL19-4886 + CCGCGUUGUCUAGCAGCG 18 9269

HSV2-UL19-4887 + GCCGCGUUGUCUAGCAGCG 19 9270

HSV2-UL19-1190 + GGCCGCGUUGUCUAGCAGCG 20 1430

HSV2-UL19-4888 + CGGCCGCGUUGUCUAGCAGCG 21 9271

HSV2-UL19-4889 + GCGGCCGCGUUGUCUAGCAGCG 22 9272

HSV2-UL19-4890 + GGCGGCCGCGUUGUCUAGCAGCG 23 9273

HSV2-UL19-4891 + CGGCGGCCGCGUUGUCUAGCAGCG 24 9274

HSV2-UL19-4892 + GAGACAGGCCCU UUAGCG 18 9275

HSV2-UL19-4893 + AGAGACAGGCCCUUUAGCG 19 9276

HSV2-UL19-1103 + CAGAGACAGGCCCUUUAGCG 20 1343

HSV2-UL19-4894 + ACAGAGACAGGCCCUUUAGCG 21 9277

HSV2-UL19-4895 + UACAGAGACAGGCCCUUUAGCG 22 9278

HSV2-UL19-4896 + UU ACAGAGACAGGCCCUUUAGCG 23 9279

HSV2-UL19-4897 + UUUACAGAGACAGGCCCUUUAGCG 24 9280

HSV2-UL19-4898 + UGCCGGGGAGGGAACGCG 18 9281

HSV2-UL19-4899 + CUGCCGGGGAGGGAACGCG 19 9282

HSV2-UL19-1451 + GCUGCCGGGGAGGGAACGCG 20 1691

HSV2-UL19-4900 + AGCUGCCGGGGAGGGAACGCG 21 9283

HSV2-UL19-4901 + CAGCUGCCGGGGAGGGAACGCG 22 9284

HSV2-UL19-4902 + ACAGCUGCCGGGGAGGGAACGCG 23 9285

HSV2-UL19-4903 + AACAGCUGCCGGGGAGGGAACGCG 24 9286

HSV2-UL19-4904 + ACGUCGGCCACGCCCGCG 18 9287

HSV2-UL19-4905 + GACGUCGGCCACGCCCGCG 19 9288

HSV2-UL19-2701 + CGACGUCGGCCACGCCCGCG 20 2809

HSV2-UL19-4906 + UCGACGUCGGCCACGCCCGCG 21 9289

HSV2-UL19-4907 + CUCGACGUCGGCCACGCCCGCG 22 9290

HSV2-UL19-4908 + GCUCGACGUCGGCCACGCCCGCG 23 9291

HSV2-UL19-4909 + AGCUCGACGUCGGCCACGCCCGCG 24 9292

HSV2-UL19-4910 + GCGGUGUUGGCGCCCGCG 18 9293

HSV2-UL19-4911 + CGCGGUGUUGGCGCCCGCG 19 9294

HSV2-UL19-2628 + ACGCGGUGUUGGCGCCCGCG 20 2755

HSV2-UL19-4912 + GACGCGGUGUUGGCGCCCGCG 21 9295

HSV2-UL19-4913 + CGACGCGGUGUUGGCGCCCGCG 22 9296 HSV2-UL19-4914 + UCGACGCGGUGUUGGCGCCCGCG 23 9297

HSV2-UL19-4915 + GUCGACGCGGUGUUGGCGCCCGCG 24 9298

HSV2-UL19-4916 + GCCGUGAUGUCGGCCGCG 18 9299

HSV2-UL19-4917 + GGCCGUGAUGUCGGCCGCG 19 9300

HSV2-UL19-2523 + UGGCCGUGAUGUCGGCCGCG 20 2687

HSV2-UL19-4918 + UUGGCCGUGAUGUCGGCCGCG 21 9301

HSV2-UL19-4919 + UUUGGCCGUGAUGUCGGCCGCG 22 9302

HSV2-UL19-4920 + GUU UGGCCGUGAUGUCGGCCGCG 23 9303

HSV2-UL19-4921 + UGU UUGGCCGUGAUGUCGGCCGCG 24 9304

HSV2-UL19-4922 + GGGUUGGCCGUGGCCGCG 18 9305

HSV2-UL19-4923 + CGGGUUGGCCGUGGCCGCG 19 9306

HSV2-UL19-2537 + ACGGGUUGGCCGUGGCCGCG 20 2700

HSV2-UL19-4924 + CACGGGUUGGCCGUGGCCGCG 21 9307

HSV2-UL19-4925 + CCACGGGUUGGCCGUGGCCGCG 22 9308

HSV2-UL19-4926 + CCCACGGGUUGGCCGUGGCCGCG 23 9309

HSV2-UL19-4927 + GCCCACGGGUUGGCCGUGGCCGCG 24 9310

HSV2-UL19-4928 + CGCUGCUGCAUGUCCGCG 18 9311

HSV2-UL19-4929 + ACGCUGCUGCAUGUCCGCG 19 9312

HSV2-UL19-2712 + AACGCUGCUGCAUGUCCGCG 20 2819

HSV2-UL19-4930 + AAACGCUGCUGCAUGUCCGCG 21 9313

HSV2-UL19-4931 + AAAACGCUGCUGCAUGUCCGCG 22 9314

HSV2-UL19-4932 + GAAAACGCUGCUGCAUGUCCGCG 23 9315

HSV2-UL19-4933 + AGAAAACGCUGCUGCAUGUCCGCG 24 9316

HSV2-UL19-4934 + AGGUCCCCGGCGUGCGCG 18 9317

HSV2-UL19-4935 + CAGGUCCCCGGCGUGCGCG 19 9318

HSV2-UL19-2738 + CCAGGUCCCCGGCGUGCGCG 20 2834

HSV2-UL19-4936 + ACCAGGUCCCCGGCGUGCGCG 21 9319

HSV2-UL19-4937 + CACCAGGUCCCCGGCGUGCGCG 22 9320

HSV2-UL19-4938 + GCACCAGGUCCCCGGCGUGCGCG 23 9321

HSV2-UL19-4939 + GGCACCAGGUCCCCGGCGUGCGCG 24 9322

HSV2-UL19-4940 + GUCGCGCGCCAGGUCGCG 18 9323

HSV2-UL19-4941 + CGUCGCGCGCCAGGUCGCG 19 9324

HSV2-UL19-2609 + ACGUCGCGCGCCAGGUCGCG 20 2744

HSV2-UL19-4942 + GACGUCGCGCGCCAGGUCGCG 21 9325

HSV2-UL19-4943 + GG ACGUCGCGCGCCAGGUCGCG 22 9326

HSV2-UL19-4944 + GGGACGUCGCGCGCCAGGUCGCG 23 9327

HSV2-UL19-4945 + GGGGACGUCGCGCGCCAGGUCGCG 24 9328

HSV2-UL19-4946 + ACGAGCCGCGCCAGGGCG 18 9329

HSV2-UL19-4947 + CACGAGCCGCGCCAGGGCG 19 9330

HSV2-UL19-2679 + UCACGAGCCGCGCCAGGGCG 20 2794

HSV2-UL19-4948 + GUCACGAGCCGCGCCAGGGCG 21 9331

HSV2-UL19-4949 + AG UCACGAGCCGCGCCAGGGCG 22 9332

HSV2-UL19-4950 + GAGU CACGAGCCGCGCCAGGGCG 23 9333

HSV2-UL19-4951 + UGAGUCACGAGCCGCGCCAGGGCG 24 9334

HSV2-UL19-4952 + GUGGCCACGGGGGUGGCG 18 9335

HSV2-UL19-4953 + CGUGGCCACGGGGGUGGCG 19 9336 HSV2-UL19-2547 + CCGUGGCCACGGGGGUGGCG 20 2708

HSV2-UL19-4954 + UCCGUGGCCACGGGGGUGGCG 21 9337

HSV2-UL19-4955 + GUCCGUGGCCACGGGGGUGGCG 22 9338

HSV2-UL19-4956 + UGUCCGUGGCCACGGGGGUGGCG 23 9339

HSV2-UL19-4957 + AUGUCCGUGGCCACGGGGGUGGCG 24 9340

HSV2-UL19-4958 + UACGGAUUCGCGGCCUCG 18 9341

HSV2-UL19-4959 + GUACGGAUUCGCGGCCUCG 19 9342

HSV2-UL19-2717 + CGUACGGAUUCGCGGCCUCG 20 2822

HSV2-UL19-4960 + CCGUACGGAUUCGCGGCCUCG 21 9343

HSV2-UL19-4961 + CCCGUACGGAU UCGCGGCCUCG 22 9344

HSV2-UL19-4962 + CCCCGUACGGAUUCGCGGCCUCG 23 9345

HSV2-UL19-4963 + GCCCCGUACGGAU UCGCGGCCUCG 24 9346

HSV2-UL19-4964 + ACGGCCAUGCACUCCUCG 18 9347

HSV2-UL19-4965 + CACGGCCAUGCACUCCUCG 19 9348

HSV2-UL19-1370 + ACACGGCCAUGCACUCCUCG 20 1610

HSV2-UL19-4966 + UACACGGCCAUGCACUCCUCG 21 9349

HSV2-UL19-4967 + AUACACGGCCAUGCACUCCUCG 22 9350

HSV2-UL19-4968 + GAUACACGGCCAUGCACUCCUCG 23 9351

HSV2-UL19-4969 + CGAUACACGGCCAUGCACUCCUCG 24 9352

HSV2-UL19-4970 + UGGCCCGCCUUGCCCAGG 18 9353

HSV2-UL19-4971 + AUGGCCCGCCUUGCCCAGG 19 9354

HSV2-UL19-2770 + GAUGGCCCGCCUUGCCCAGG 20 2855

HSV2-UL19-4972 + CGAUGGCCCGCCUUGCCCAGG 21 9355

HSV2-UL19-4973 + GCGAUGGCCCGCCUUGCCCAGG 22 9356

HSV2-UL19-4974 + GGCGAUGGCCCGCCUUGCCCAGG 23 9357

HSV2-UL19-4975 + CGGCGAUGGCCCGCCUUGCCCAGG 24 9358

HSV2-UL19-4976 + CGGGCGCUGCGUAGCAGG 18 9359

HSV2-UL19-4977 + GCGGGCGCUGCGUAGCAGG 19 9360

HSV2-UL19-2514 + CGCGGGCGCUGCGUAGCAGG 20 2679

HSV2-UL19-4978 + UCGCGGGCGCUGCGUAGCAGG 21 9361

HSV2-UL19-4979 + AU CGCGGGCGCUGCGUAGCAGG 22 9362

HSV2-UL19-4980 + CAUCGCGGGCGCUGCGUAGCAGG 23 9363

HSV2-UL19-4981 + CCAUCGCGGGCGCUGCGUAGCAGG 24 9364

HSV2-UL19-4982 + AACAGCCCCAGGGGCAGG 18 9365

HSV2-UL19-4983 + GAACAGCCCCAGGGGCAGG 19 9366

HSV2-UL19-2744 + UG AACAGCCCCAGGGGCAGG 20 2839

HSV2-UL19-4984 + UUGAACAGCCCCAGGGGCAGG 21 9367

HSV2-UL19-4985 + GUU GAACAGCCCCAGGGGCAGG 22 9368

HSV2-UL19-4986 + GGUUGAACAGCCCCAGGGGCAGG 23 9369

HSV2-UL19-4987 + GGGUUGAACAGCCCCAGGGGCAGG 24 9370

HSV2-UL19-4988 + GUGUCGGCGUGCGUCAGG 18 9371

HSV2-UL19-4989 + CGUGUCGGCGUGCGUCAGG 19 9372

HSV2-UL19-1516 + GCGUGUCGGCGUGCGUCAGG 20 1756

HSV2-UL19-4990 + CGCGUGUCGGCGUGCGUCAGG 21 9373

HSV2-UL19-4991 + GCGCGUGUCGGCGUGCGUCAGG 22 9374

HSV2-UL19-4992 + CGCGCGUGUCGGCGUGCGUCAGG 23 9375 HSV2-UL19-4993 + CCGCGCGUGUCGGCGUGCGUCAGG 24 9376

HSV2-UL19-4994 + G U G U CCU CCACCU UCAG G 18 9377

HSV2-UL19-4995 + GGUGUCCUCCACCUUCAGG 19 9378

HSV2-UL19-2761 + CGGUGUCCUCCACCUUCAGG 20 2849

HSV2-UL19-4996 + UCGGUGUCCUCCACCUUCAGG 21 9379

HSV2-UL19-4997 + CUCGGUGUCCUCCACCUUCAGG 22 9380

HSV2-UL19-4998 + CCUCGGUGUCCUCCACCUUCAGG 23 9381

HSV2-UL19-4999 + GCCUCGGUGUCCUCCACCUUCAGG 24 9382

HSV2-UL19-5000 + UUGAAGCAGGGGCUGAGG 18 9383

HSV2-UL19-5001 + CUUGAAGCAGGGGCUGAGG 19 9384

HSV2-UL19-2530 + ACUUGAAGCAGGGGCUGAGG 20 2693

HSV2-UL19-5002 + AACUUGAAGCAGGGGCUGAGG 21 9385

HSV2-UL19-5003 + GAACUUGAAGCAGGGGCUGAGG 22 9386

HSV2-UL19-5004 + AGAACUUGAAGCAGGGGCUGAGG 23 9387

HSV2-UL19-5005 + AAGAACUUGAAGCAGGGGCUGAGG 24 9388

HSV2-UL19-5006 + AGCAGGGGCUGAGGACGG 18 9389

HSV2-UL19-5007 + AAGCAGGGGCUGAGGACGG 19 9390

HSV2-UL19-2531 + GAAGCAGGGGCUGAGGACGG 20 2694

HSV2-UL19-5008 + UGAAGCAGGGGCUGAGGACGG 21 9391

HSV2-UL19-5009 + UU GAAGCAGGGGCUGAGGACGG 22 9392

HSV2-UL19-5010 + CU UGAAGCAGGGGCUGAGGACGG 23 9393

HSV2-UL19-5011 + ACUUGAAGCAGGGGCUGAGGACGG 24 9394

HSV2-UL19-5012 + GGCGCAGCCAAAGACCGG 18 9395

HSV2-UL19-5013 + GGGCGCAGCCAAAGACCGG 19 9396

HSV2-UL19-2552 + U G G G CG CAG CCAAAG ACCG G 20 2711

HSV2-UL19-5014 + CUGGGCGCAGCCAAAGACCGG 21 9397

HSV2-UL19-5015 + CCUGGGCGCAGCCAAAGACCGG 22 9398

HSV2-UL19-5016 + ACCUGGGCGCAGCCAAAGACCGG 23 9399

HSV2-UL19-5017 + CACCUGGGCGCAGCCAAAGACCGG 24 9400

HSV2-UL19-5018 + GGAUCGCCCCCGGACCGG 18 9401

HSV2-UL19-5019 + UGGAUCGCCCCCGGACCGG 19 9402

HSV2-UL19-2692 + CUGGAUCGCCCCCGGACCGG 20 2806

HSV2-UL19-5020 + CCUGGAUCGCCCCCGGACCGG 21 9403

HSV2-UL19-5021 + GCCUGGAUCGCCCCCGGACCGG 22 9404

HSV2-UL19-5022 + GGCCUGGAUCGCCCCCGGACCGG 23 9405

HSV2-UL19-5023 + UGGCCUGGAUCGCCCCCGGACCGG 24 9406

HSV2-UL19-5024 + CGCCCCCGGACCGGCCGG 18 9407

HSV2-UL19-5025 + UCGCCCCCGGACCGGCCGG 19 9408

HSV2-UL19-1406 + AUCGCCCCCGGACCGGCCGG 20 1646

HSV2-UL19-5026 + GAUCGCCCCCGGACCGGCCGG 21 9409

HSV2-UL19-5027 + GGAUCGCCCCCGGACCGGCCGG 22 9410

HSV2-UL19-5028 + UGGAUCGCCCCCGGACCGGCCGG 23 9411

HSV2-UL19-5029 + CUGGAUCGCCCCCGGACCGGCCGG 24 9412

HSV2-UL19-5030 + GUUGGCCCCCAGGGCCGG 18 9413

HSV2-UL19-5031 + AGUUGGCCCCCAGGGCCGG 19 9414

HSV2-UL19-1248 + UAGUUGGCCCCCAGGGCCGG 20 1488 HSV2-UL19-5032 + GUAGUUGGCCCCCAGGGCCGG 21 9415

HSV2-UL19-5033 + AGUAGUUGGCCCCCAGGGCCGG 22 9416

HSV2-UL19-5034 + AAGUAGUUGGCCCCCAGGGCCGG 23 9417

HSV2-UL19-5035 + GAAGUAGUUGGCCCCCAGGGCCGG 24 9418

HSV2-UL19-5036 + CAGGUCGCGCAGGGCCGG 18 9419

HSV2-UL19-5037 + CCAGGUCGCGCAGGGCCGG 19 9420

HSV2-UL19-1260 + GCCAGGUCGCGCAGGGCCGG 20 1500

HSV2-UL19-5038 + CGCCAGGUCGCGCAGGGCCGG 21 9421

HSV2-UL19-5039 + GCGCCAGGUCGCGCAGGGCCGG 22 9422

HSV2-UL19-5040 + CGCGCCAGGUCGCGCAGGGCCGG 23 9423

HSV2-UL19-5041 + GCGCGCCAGGUCGCGCAGGGCCGG 24 9424

HSV2-UL19-5042 + UGUCCGCGCCGGGGCCGG 18 9425

HSV2-UL19-5043 + AUGUCCGCGCCGGGGCCGG 19 9426

HSV2-UL19-2714 + CAUGUCCGCGCCGGGGCCGG 20 2820

HSV2-UL19-5044 + GCAUGUCCGCGCCGGGGCCGG 21 9427

HSV2-UL19-5045 + UGCAUGUCCGCGCCGGGGCCGG 22 9428

HSV2-UL19-5046 + CUGCAUGUCCGCGCCGGGGCCGG 23 9429

HSV2-UL19-5047 + GCUGCAUGUCCGCGCCGGGGCCGG 24 9430

HSV2-UL19-5048 + CGCGUUGUCUAGCAGCGG 18 9431

HSV2-UL19-5049 + CCGCGUUGUCUAGCAGCGG 19 9432

HSV2-UL19-1191 + GCCGCGUUGUCUAGCAGCGG 20 1431

HSV2-UL19-5050 + GGCCGCGUUGUCUAGCAGCGG 21 9433

HSV2-UL19-5051 + CGGCCGCGUUGUCUAGCAGCGG 22 9434

HSV2-UL19-5052 + GCGGCCGCGUUGUCUAGCAGCGG 23 9435

HSV2-UL19-5053 + GGCGGCCGCGUUGUCUAGCAGCGG 24 9436

HSV2-UL19-5054 + AUUGGCGGGAUGCAGCGG 18 9437

HSV2-UL19-5055 + GAU UGGCGGGAUGCAGCGG 19 9438

HSV2-UL19-2638 + AG AUUGGCGGGAUGCAGCGG 20 2762

HSV2-UL19-5056 + CAG AU UGGCGGGAUGCAGCGG 21 9439

HSV2-UL19-5057 + CCAGAUUGGCGGGAUGCAGCGG 22 9440

HSV2-UL19-5058 + ACCAGAUUGGCGGGAUGCAGCGG 23 9441

HSV2-UL19-5059 + CACCAGAU UGGCGGGAUGCAGCGG 24 9442

HSV2-UL19-5060 + AGCGUGGCGUACACGCGG 18 9443

HSV2-UL19-5061 + CAGCGUGGCGUACACGCGG 19 9444

HSV2-UL19-2649 + GCAGCGUGGCGUACACGCGG 20 2768

HSV2-UL19-5062 + UGCAGCGUGGCGUACACGCGG 21 9445

HSV2-UL19-5063 + CUGCAGCGUGGCGUACACGCGG 22 9446

HSV2-UL19-5064 + UCUGCAGCGUGGCGUACACGCGG 23 9447

HSV2-UL19-5065 + UUCUGCAGCGUGGCGUACACGCGG 24 9448

HSV2-UL19-5066 + AGCGGGGGGGCCCCGCGG 18 9449

HSV2-UL19-5067 + CAGCGGGGGGGCCCCGCGG 19 9450

HSV2-UL19-2560 + GCAGCGGGGGGGCCCCGCGG 20 2714

HSV2-UL19-5068 + AGCAGCGGGGGGGCCCCGCGG 21 9451

HSV2-UL19-5069 + UAGCAGCGGGGGGGCCCCGCGG 22 9452

HSV2-UL19-5070 + CUAGCAGCGGGGGGGCCCCGCGG 23 9453

HSV2-UL19-5071 + UCUAGCAGCGGGGGGGCCCCGCGG 24 9454 HSV2-UL19-5072 + UGCACCACGGGCUGGCGG 18 9455

HSV2-UL19-5073 + CUGCACCACGGGCUGGCGG 19 9456

HSV2-UL19-2594 + GCUGCACCACGGGCUGGCGG 20 2738

HSV2-UL19-5074 + UGCUGCACCACGGGCUGGCGG 21 9457

HSV2-UL19-5075 + GUGCUGCACCACGGGCUGGCGG 22 9458

HSV2-UL19-5076 + CGUGCUGCACCACGGGCUGGCGG 23 9459

HSV2-UL19-5077 + GCGUGCUGCACCACGGGCUGGCGG 24 9460

HSV2-UL19-5078 + AGUAGUUGGCCCCCAGGG 18 9461

HSV2-UL19-5079 + AAGUAGUUGGCCCCCAGGG 19 9462

HSV2-UL19-2600 + GAAGUAGUUGGCCCCCAGGG 20 2742

HSV2-UL19-5080 + AG AAGUAGUUGGCCCCCAGGG 21 9463

HSV2-UL19-5081 + GAGAAGUAGUUGGCCCCCAGGG 22 9464

HSV2-UL19-5082 + GG AG AAGUAGUUGGCCCCCAGGG 23 9465

HSV2-UL19-5083 + AGGAGAAGUAGUUGGCCCCCAGGG 24 9466

HSV2-UL19-5084 + GCGCCAGGUCGCGCAGGG 18 9467

HSV2-UL19-5085 + CGCGCCAGGUCGCGCAGGG 19 9468

HSV2-UL19-2610 + GCGCGCCAGGUCGCGCAGGG 20 2745

HSV2-UL19-5086 + CGCGCGCCAGGUCGCGCAGGG 21 9469

HSV2-UL19-5087 + UCGCGCGCCAGGUCGCGCAGGG 22 9470

HSV2-UL19-5088 + GUCGCGCGCCAGGUCGCGCAGGG 23 9471

HSV2-UL19-5089 + CGUCGCGCGCCAGGUCGCGCAGGG 24 9472

HSV2-UL19-5090 + AAACAGCGCGUGGACGGG 18 9473

HSV2-UL19-5091 + CAAACAGCGCGUGGACGGG 19 9474

HSV2-UL19-2619 + G CAAACAG CGCGUGGACGGG 20 2749

HSV2-UL19-5092 + CGCAAACAGCGCGUGGACGGG 21 9475

HSV2-UL19-5093 + CCGCAAACAGCGCGUGGACGGG 22 9476

HSV2-UL19-5094 + CCCGCAAACAGCGCGUGGACGGG 23 9477

HSV2-UL19-5095 + GCCCGCAAACAGCGCGUGGACGGG 24 9478

HSV2-UL19-5096 + GCGCAGCCAAAGACCGGG 18 9479

HSV2-UL19-5097 + GGCGCAGCCAAAGACCGGG 19 9480

HSV2-UL19-1178 + GGGCGCAGCCAAAGACCGGG 20 1418

HSV2-UL19-5098 + UGGGCGCAGCCAAAGACCGGG 21 9481

HSV2-UL19-5099 + CUGGGCGCAGCCAAAGACCGGG 22 9482

HSV2-UL19-5100 + CCUGGGCGCAGCCAAAGACCGGG 23 9483

HSV2-UL19-5101 + ACCUGGGCGCAGCCAAAGACCGGG 24 9484

HSV2-UL19-5102 + GGUGAACCCGAACCCGGG 18 9485

HSV2-UL19-5103 + CGGUGAACCCGAACCCGGG 19 9486

HSV2-UL19-2583 + ACGGUGAACCCGAACCCGGG 20 2729

HSV2-UL19-5104 + GACGGUGAACCCGAACCCGGG 21 9487

HSV2-UL19-5105 + CGACGGUGAACCCGAACCCGGG 22 9488

HSV2-UL19-5106 + ACG ACGGUGAACCCGAACCCGGG 23 9489

HSV2-UL19-5107 + CACGACG G U G AACCCG AACCCGG G 24 9490

HSV2-UL19-5108 + AGGUCGCGCAGGGCCGGG 18 9491

HSV2-UL19-5109 + CAGGUCGCGCAGGGCCGGG 19 9492

HSV2-UL19-1261 + CCAGGUCGCGCAGGGCCGGG 20 1501

HSV2-UL19-5110 + GCCAGGUCGCGCAGGGCCGGG 21 9493 HSV2-UL19-5111 + CGCCAGGUCGCGCAGGGCCGGG 22 9494

HSV2-UL19-5112 + GCGCCAGGUCGCGCAGGGCCGGG 23 9495

HSV2-UL19-5113 + CGCGCCAGGUCGCGCAGGGCCGGG 24 9496

HSV2-UL19-5114 + AAGCGCUCCAUGGCCGGG 18 9497

HSV2-UL19-5115 + GAAGCGCUCCAUGGCCGGG 19 9498

HSV2-UL19-2740 + CGAAGCGCUCCAUGGCCGGG 20 2836

HSV2-UL19-5116 + GCG AAGCGCUCCAUGGCCGGG 21 9499

HSV2-UL19-5117 + GGCGAAGCGCUCCAUGGCCGGG 22 9500

HSV2-UL19-5118 + CGGCGAAGCGCUCCAUGGCCGGG 23 9501

HSV2-UL19-5119 + GCGGCGAAGCGCUCCAUGGCCGGG 24 9502

HSV2-UL19-5120 + AAAAAAACAGCUGCCGGG 18 9503

HSV2-UL19-5121 + CAAAAAAACAG CU G CCG G G 19 9504

HSV2-UL19-2728 + CCAAAAAAACAG CU G CCG G G 20 2829

HSV2-UL19-5122 + CCCAAAAAAACAGCUGCCGGG 21 9505

HSV2-UL19-5123 + CCCCAAAAAAACAGCUGCCGGG 22 9506

HSV2-UL19-5124 + U CCCCAAAAAAACAG CU G CCG G G 23 9507

HSV2-UL19-5125 + U U CCCCAAAAAAACAG CU GCCGGG 24 9508

HSV2-UL19-5126 + GUUGAACAGCCCCAGGGG 18 9509

HSV2-UL19-5127 + GGU UGAACAGCCCCAGGGG 19 9510

HSV2-UL19-2743 + GGGUUGAACAGCCCCAGGGG 20 2838

HSV2-UL19-5128 + CGGGUUGAACAGCCCCAGGGG 21 9511

HSV2-UL19-5129 + CCGGGUUGAACAGCCCCAGGGG 22 9512

HSV2-UL19-5130 + GCCGGGUUGAACAGCCCCAGGGG 23 9513

HSV2-UL19-5131 + GGCCGGGUUGAACAGCCCCAGGGG 24 9514

HSV2-UL19-5132 + AAGAACUUGAAGCAGGGG 18 9515

HSV2-UL19-5133 + GAAGAACUUGAAGCAGGGG 19 9516

HSV2-UL19-2528 + UGAAGAACUUGAAGCAGGGG 20 2691

HSV2-UL19-5134 + GUG AAGAACUUGAAGCAGGGG 21 9517

HSV2-UL19-5135 + GGUG AAGAACUUGAAGCAGGGG 22 9518

HSV2-UL19-5136 + CGGUGAAGAACUUGAAGCAGGGG 23 9519

HSV2-UL19-5137 + GCGGUGAAGAACUUGAAGCAGGGG 24 9520

HSV2-UL19-5138 + GCCAAAGACCGGGAGGGG 18 9521

HSV2-UL19-5139 + AGCCAAAGACCGGGAGGGG 19 9522

HSV2-UL19-2554 + CAGCCAAAGACCGGGAGGGG 20 2712

HSV2-UL19-5140 + GCAGCCAAAGACCGGGAGGGG 21 9523

HSV2-UL19-5141 + CG CAG CCAAAG ACCGGGAGGGG 22 9524

HSV2-UL19-5142 + GCGCAGCCAAAGACCGGGAGGGG 23 9525

HSV2-UL19-5143 + GGCGCAGCCAAAGACCGGGAGGGG 24 9526

HSV2-UL19-5144 + GUGAACCCGAACCCGGGG 18 9527

HSV2-UL19-5145 + GGUGAACCCGAACCCGGGG 19 9528

HSV2-UL19-1225 + CGGUGAACCCGAACCCGGGG 20 1465

HSV2-UL19-5146 + ACG G U G AACCCG AACCCG GGG 21 9529

HSV2-UL19-5147 + GACGGUGAACCCGAACCCGGGG 22 9530

HSV2-UL19-5148 + CGACGGUGAACCCGAACCCGGGG 23 9531

HSV2-UL19-5149 + ACGACGGUGAACCCGAACCCGGGG 24 9532

HSV2-UL19-5150 + GGCUCCGGGUGGCCGGGG 18 9533 HSV2-UL19-5151 + GGGCUCCGGGUGGCCGGGG 19 9534

HSV2-UL19-2737 + GGGGCUCCGGGUGGCCGGGG 20 2833

HSV2-UL19-5152 + CGGGGCUCCGGGUGGCCGGGG 21 9535

HSV2-UL19-5153 + GCGGGGCUCCGGGUGGCCGGGG 22 9536

HSV2-UL19-5154 + CGCGGGGCUCCGGGUGGCCGGGG 23 9537

HSV2-UL19-5155 + GCGCGGGGCUCCGGGUGGCCGGGG 24 9538

HSV2-UL19-5156 + AAAAAACAG CUGCCGGGG 18 9539

HSV2-UL19-5157 + AAAAAAACAGCUGCCGGGG 19 9540

HSV2-UL19-1449 + CAAAAAAACAG CUGCCGGGG 20 1689

HSV2-UL19-5158 + CCAAAA AAAC AG CU G CCG G G G 21 9541

HSV2-UL19-5159 + CCCAAAAAAACAG CU G CCG G G G 22 9542

HSV2-UL19-5160 + CCCCAAAAAAACAG CU G CCG G G G 23 9543

HSV2-UL19-5161 + UCCCCAAAAAAACAGCUGCCGGGG 24 9544

HSV2-UL19-5162 + AUGGCGCCCACCAGGGGG 18 9545

HSV2-UL19-5163 + CAUGGCGCCCACCAGGGGG 19 9546

HSV2-UL19-2748 + CCAUGGCGCCCACCAGGGGG 20 2841

HSV2-UL19-5164 + UCCAUGGCGCCCACCAGGGGG 21 9547

HSV2-UL19-5165 + GUCCAUGGCGCCCACCAGGGGG 22 9548

HSV2-UL19-5166 + GGUCCAUGGCGCCCACCAGGGGG 23 9549

HSV2-UL19-5167 + AGGUCCAUGGCGCCCACCAGGGGG 24 9550

HSV2-UL19-5168 + CCACGGGCUGGCGGAUGG 18 9551

HSV2-UL19-5169 + ACCACGGGCUGGCGGAUGG 19 9552

HSV2-UL19-1241 + CACCACGGGCUGGCGGAUGG 20 1481

HSV2-UL19-5170 + GCACCACGGGCUGGCGGAUGG 21 9553

HSV2-UL19-5171 + UGCACCACGGGCUGGCGGAUGG 22 9554

HSV2-UL19-5172 + CUGCACCACGGGCUGGCGGAUGG 23 9555

HSV2-UL19-5173 + GCUGCACCACGGGCUGGCGGAUGG 24 9556

HSV2-UL19-5174 + ACGUUUCCGCGCGGCUGG 18 9557

HSV2-UL19-5175 + CACGUU UCCGCGCGGCUGG 19 9558

HSV2-UL19-2570 + CCACGUUUCCGCGCGGCUGG 20 2719

HSV2-UL19-5176 + UCCACGUUUCCGCGCGGCUGG 21 9559

HSV2-UL19-5177 + GUCCACGUUUCCGCGCGGCUGG 22 9560

HSV2-UL19-5178 + GGUCCACGU UUCCGCGCGGCUGG 23 9561

HSV2-UL19-5179 + AGGUCCACGUUUCCGCGCGGCUGG 24 9562

HSV2-UL19-5180 + CACCGGACCCGGCCGUGG 18 9563

HSV2-UL19-5181 + CCACCGGACCCGGCCGUGG 19 9564

HSV2-UL19-2710 + CCCACCGGACCCGGCCGUGG 20 2817

HSV2-UL19-5182 + ACCCACCGGACCCGGCCGUGG 21 9565

HSV2-UL19-5183 + GACCCACCGGACCCGGCCGUGG 22 9566

HSV2-UL19-5184 + CGACCCACCGGACCCGGCCGUGG 23 9567

HSV2-UL19-5185 + GCGACCCACCGGACCCGGCCGUGG 24 9568

HSV2-UL19-5186 + CCCGCAAACAGCGCGUGG 18 9569

HSV2-UL19-5187 + GCCCGCAAACAGCGCGUGG 19 9570

HSV2-UL19-2618 + CGCCCGCAAACAGCGCGUGG 20 2748

HSV2-UL19-5188 + GCGCCCGCAAACAGCGCGUGG 21 9571

HSV2-UL19-5189 + GGCGCCCGCAAACAGCGCGUGG 22 9572 HSV2-UL19-5190 + CGGCGCCCGCAAACAGCGCGUGG 23 9573

HSV2-UL19-5191 + UCGGCGCCCGCAAACAGCGCGUGG 24 9574

HSV2-UL19-5192 + GCGCGGGGCUCCGGGUGG 18 9575

HSV2-UL19-5193 + CGCGCGGGGCUCCGGGUGG 19 9576

HSV2-UL19-2734 + ACGCGCGGGGCUCCGGGUGG 20 2832

HSV2-UL19-5194 + AACGCGCGGGGCUCCGGGUGG 21 9577

HSV2-UL19-5195 + GAACGCGCGGGGCUCCGGGUGG 22 9578

HSV2-UL19-5196 + GGAACGCGCGGGGCUCCGGGUGG 23 9579

HSV2-UL19-5197 + GGGAACGCGCGGGGCUCCGGGUGG 24 9580

HSV2-UL19-5198 + GCGGGAUGCAGCGGGUGG 18 9581

HSV2-UL19-5199 + GGCGGGAUGCAGCGGGUGG 19 9582

HSV2-UL19-2639 + UGGCGGGAUGCAGCGGGUGG 20 2763

HSV2-UL19-5200 + UU GGCGGGAUGCAGCGGGUGG 21 9583

HSV2-UL19-5201 + AU UGGCGGGAUGCAGCGGGUGG 22 9584

HSV2-UL19-5202 + GAU UGGCGGGAUGCAGCGGGUGG 23 9585

HSV2-UL19-5203 + AGAUUGGCGGGAUGCAGCGGGUGG 24

9586

HSV2-UL19-5204 + CCGAGGUAAAAGUUUUGG 18 9587

HSV2-UL19-5205 + GCCGAGGUAAAAGUUUUGG 19 9588

HSV2-UL19-1199 + GGCCGAGGUAAAAGUUUUGG 20 1439

HSV2-UL19-5206 + CGGCCGAGGUAAAAGUUUUGG 21 9589

HSV2-UL19-5207 + GCGGCCGAGGUAAAAGUUUUGG 22 9590

HSV2-UL19-5208 + CGCGGCCGAGGUAAAAGUUUUGG 23 9591

HSV2-UL19-5209 + CCGCGGCCGAGGUAAAAGUUUUGG 24 9592

HSV2-UL19-5210 + ACCUGCAGCGUGAGCAUG 18 9593

HSV2-UL19-5211 + CACCUGCAGCGUGAGCAUG 19 9594

HSV2-UL19-2632 + GCACCUGCAGCGUGAGCAUG 20 2758

HSV2-UL19-5212 + AGCACCUGCAGCGUGAGCAUG 21 9595

HSV2-UL19-5213 + CAGCACCUGCAGCGUGAGCAUG 22 9596

HSV2-UL19-5214 + CCAGCACCUGCAGCGUGAGCAUG 23 9597

HSV2-UL19-5215 + GCCAGCACCUGCAGCGUGAGCAUG 24 9598

HSV2-UL19-5216 + ACCACGGGCUGGCGGAUG 18 9599

HSV2-UL19-5217 + CACCACGGGCUGGCGGAUG 19 9600

HSV2-UL19-2596 + GCACCACGGGCUGGCGGAUG 20 2739

HSV2-UL19-5218 + UGCACCACGGGCUGGCGGAUG 21 9601

HSV2-UL19-5219 + CUGCACCACGGGCUGGCGGAUG 22 9602

HSV2-UL19-5220 + GCUGCACCACGGGCUGGCGGAUG 23 9603

HSV2-UL19-5221 + UGCUGCACCACGGGCUGGCGGAUG 24 9604

HSV2-UL19-5222 + CACCUUCAGGAAGGACUG 18 9605

HSV2-UL19-5223 + CCACCUUCAGGAAGGACUG 19 9606

HSV2-UL19-2762 + UCCACCUUCAGGAAGGACUG 20 2850

HSV2-UL19-5224 + CUCCACCUUCAGGAAGGACUG 21 9607

HSV2-UL19-5225 + CCUCCACCUUCAGGAAGGACUG 22 9608

HSV2-UL19-5226 + UCCUCCACCUUCAGGAAGGACUG 23 9609

HSV2-UL19-5227 + GUCCUCCACCUUCAGGAAGGACUG 24 9610

HSV2-UL19-5228 + U CCCCAAAAAAACAG CU G 18 9611 HSV2-UL19-5229 + U U CCCCAAAAAAACAG CU G 19 9612

HSV2-UL19-2724 + UUUCCCCAAAAAAACAGCUG 20 2828

HSV2-UL19-5230 + CU U U CCCCAAAAAAACAG CU G 21 9613

HSV2-UL19-5231 + CCU U U CCCCAAAAAAACAGCU G 22 9614

HSV2-UL19-5232 + UCCUU UCCCCAAAAAAACAGCUG 23 9615

HSV2-UL19-5233 + G U CCU U U CCCCAAAAAAACAGCU G 24 9616

HSV2-UL19-5234 + GGUGUGGUCCAGGUGCUG 18 9617

HSV2-UL19-5235 + UGGUGUGGUCCAGGUGCUG 19 9618

HSV2-UL19-1277 + AUGGUGUGGUCCAGGUGCUG 20 1517

HSV2-UL19-5236 + GAU GGUGUGGUCCAGGUGCUG 21 9619

HSV2-UL19-5237 + GGAU GGUGUGGUCCAGGUGCUG 22 9620

HSV2-UL19-5238 + UGGAUGGUGUGGUCCAGGUGCUG 23 9621

HSV2-UL19-5239 + UUGGAUGGUGUGGUCCAGGUGCUG 24

9622

HSV2-UL19-5240 + GCGCACGCGCGUUGUCUG 18 9623

HSV2-UL19-5241 + CGCGCACGCGCGUUGUCUG 19 9624

HSV2-UL19-1490 + GCGCGCACGCGCGUUGUCUG 20 1730

HSV2-UL19-5242 + CGCGCGCACGCGCGUUGUCUG 21 9625

HSV2-UL19-5243 + CCGCGCGCACGCGCGUUGUCUG 22 9626

HSV2-UL19-5244 + UCCGCGCGCACGCGCGUUGUCUG 23 9627

HSV2-UL19-5245 + AUCCGCGCGCACGCGCGUUGUCUG 24 9628

HSV2-UL19-5246 + CCCAUCACCAGCGCCGUG 18 9629

HSV2-UL19-5247 + GCCCAU CACCAG CGCCGUG 19 9630

HSV2-UL19-2758 + UGCCCAUCACCAGCGCCGUG 20 2847

HSV2-UL19-5248 + UUGCCCAUCACCAGCGCCGUG 21 9631

HSV2-UL19-5249 + CU U G CCCAU CACCAG CGCCGUG 22 9632

HSV2-UL19-5250 + CCUUGCCCAUCACCAGCGCCGUG 23 9633

HSV2-UL19-5251 + GCCUUGCCCAUCACCAGCGCCGUG 24 9634

HSV2-UL19-5252 + CCCCCCGCCCGUUUCGUG 18 9635

HSV2-UL19-5253 + CCCCCCCGCCCGUUUCGUG 19 9636

HSV2-UL19-2572 + ACCCCCCCGCCCGUUUCGUG 20 2721

HSV2-UL19-5254 + GACCCCCCCGCCCGUUUCGUG 21 9637

HSV2-UL19-5255 + UGACCCCCCCGCCCGUUUCGUG 22 9638

HSV2-UL19-5256 + UUGACCCCCCCGCCCGUUUCGUG 23 9639

HSV2-UL19-5257 + GUUGACCCCCCCGCCCGUUUCGUG 24 9640

HSV2-UL19-5258 + GAUGGUGUGGUCCAGGUG 18 9641

HSV2-UL19-5259 + GGAUGGUGUGGUCCAGGUG 19 9642

HSV2-UL19-2622 + UGGAUGGUGUGGUCCAGGUG 20 2752

HSV2-UL19-5260 + UUGGAUGGUGUGGUCCAGGUG 21 9643

HSV2-UL19-5261 + UU UGGAUGGUGUGGUCCAGGUG 22 9644

HSV2-UL19-5262 + UUUUGGAUGGUGUGGUCCAGGUG 23 9645

HSV2-UL19-5263 + AUUUUGGAUGGUGUGGUCCAGGUG 24

9646

HSV2-UL19-5264 + GUGAUGUCGGCCGCGGUG 18 9647

HSV2-UL19-5265 + CGUGAUGUCGGCCGCGGUG 19 9648

HSV2-UL19-2524 + CCGUGAUGUCGGCCGCGGUG 20 2688 HSV2-UL19-5266 + GCCGUGAUGUCGGCCGCGGUG 21 9649

HSV2-UL19-5267 + GGCCGUGAUGUCGGCCGCGGUG 22 9650

HSV2-UL19-5268 + UGGCCGUGAUGUCGGCCGCGGUG 23 9651

HSV2-UL19-5269 + UUGGCCGUGAUGUCGGCCGCGGUG 24 9652

HSV2-UL19-5270 + GUAAUAGUAGAUUUUGUG 18 9653

HSV2-UL19-5271 + CGUAAUAGUAGAUUUUGUG 19 9654

HSV2-UL19-2653 + ACGUAAUAGUAGAUUUUGUG 20 2772

HSV2-UL19-5272 + CACGUAAUAGUAGAUUUUGUG 21 9655

HSV2-UL19-5273 + GCACGUAAUAGUAGAUUUUGUG 22 9656

HSV2-UL19-5274 + AGCACGUAAUAGUAGAUUUUGUG 23 9657

HSV2-UL19-5275 + CAGCACGUAAUAGUAGAUUUUGUG 24 9658

HSV2-UL19-5276 + GUGUUGGCCACCAGAUUG 18 9659

HSV2-UL19-5277 + CGUGU UGGCCACCAGAUUG 19 9660

HSV2-UL19-2635 + CCGUGUUGGCCACCAGAUUG 20 2760

HSV2-UL19-5278 + ACCGUGU UGGCCACCAGAUUG 21 9661

HSV2-UL19-5279 + GACCGUGUUGGCCACCAGAUUG 22 9662

HSV2-UL19-5280 + UGACCGUGUUGGCCACCAGAUUG 23 9663

HSV2-UL19-5281 + UUGACCGUGUUGGCCACCAGAUUG 24 9664

HSV2-UL19-5282 + ACGACGACGCGCCCGUUG 18 9665

HSV2-UL19-5283 + GACGACGACGCGCCCGUUG 19 9666

HSV2-UL19-1291 + CGACGACGACGCGCCCGUUG 20 1531

HSV2-UL19-5284 + UCGACGACGACGCGCCCGUUG 21 9667

HSV2-UL19-5285 + GUCGACGACGACGCGCCCGU UG 22 9668

HSV2-UL19-5286 + CG UCG ACGACGACGCGCCCG U UG 23 9669

HSV2-UL19-5287 + CCGUCGACGACGACGCGCCCGUUG 24 9670

HSV2-UL19-5288 + GUCGCCAGGCGCCCGUUG 18 9671

HSV2-UL19-5289 + GGUCGCCAGGCGCCCGUUG 19 9672

HSV2-UL19-2773 + UGGUCGCCAGGCGCCCGUUG 20 2858

HSV2-UL19-5290 + CU GG U CG CCAGG CG CCCG U U G 21 9673

HSV2-UL19-5291 + CCUGGUCGCCAGGCGCCCGUUG 22 9674

HSV2-UL19-5292 + CCCUGGUCGCCAGGCGCCCGUUG 23 9675

HSV2-UL19-5293 + ACCCUGGUCGCCAGGCGCCCGUUG 24 9676

HSV2-UL19-5294 + AGAGCCAGGUUGGCGUUG 18 9677

HSV2-UL19-5295 + CAGAGCCAGGUUGGCGUUG 19 9678

HSV2-UL19-2706 + CCAGAGCCAGGUUGGCGUUG 20 2813

HSV2-UL19-5296 + UCCAGAGCCAGGUUGGCGUUG 21 9679

HSV2-UL19-5297 + CUCCAGAGCCAGGUUGGCGUUG 22 9680

HSV2-UL19-5298 + GCUCCAGAGCCAGGUUGGCGUUG 23 9681

HSV2-UL19-5299 + AGCUCCAGAGCCAGGUUGGCGUUG 24 9682

HSV2-UL19-5300 + GCGCGUCCCCGCGGGUUG 18 9683

HSV2-UL19-5301 + CGCGCGUCCCCGCGGGUUG 19 9684

HSV2-UL19-2542 + CCGCGCGUCCCCGCGGGUUG 20 2705

HSV2-UL19-5302 + GCCGCGCGUCCCCGCGGGUUG 21 9685

HSV2-UL19-5303 + GGCCGCGCGUCCCCGCGGGUUG 22 9686

HSV2-UL19-5304 + CGGCCGCGCGUCCCCGCGGGUUG 23 9687

HSV2-UL19-5305 + CCGGCCGCGCGUCCCCGCGGGUUG 24 9688 HSV2-UL19-5306 + GCCGAGGUAAAAGUUUUG 18 9689

HSV2-UL19-5307 + GGCCGAGGUAAAAGUUUUG 19 9690

HSV2-UL19-1198 + CGGCCGAGGUAAAAGUUUUG 20 1438

HSV2-UL19-5308 + GCGGCCGAGGUAAAAGUUUUG 21 9691

HSV2-UL19-5309 + CGCGGCCGAGGUAAAAGUUUUG 22 9692

HSV2-UL19-5310 + CCGCGGCCGAGGUAAAAGUUUUG 23 9693

HSV2-UL19-5311 + CCCGCGGCCGAGGUAAAAGUUUUG 24 9694

HSV2-UL19-5312 + CUGGCCGUGGUCGUACAU 18 9695

HSV2-UL19-5313 + UCUGGCCGUGGUCGUACAU 19 9696

HSV2-UL19-2540 + CUCUGGCCGUGGUCGUACAU 20 2703

HSV2-UL19-5314 + GCUCUGGCCGUGGUCGUACAU 21 9697

HSV2-UL19-5315 + CGCUCUGGCCGUGGUCGUACAU 22 9698

HSV2-UL19-5316 + UCGCUCUGGCCGUGGUCGUACAU 23 9699

HSV2-UL19-5317 + GUCGCUCUGGCCGUGGUCGUACAU 24 9700

HSV2-UL19-5318 + ACACGGCCAUGCACUCCU 18 9701

HSV2-UL19-5319 + UACACGGCCAUGCACUCCU 19 9702

HSV2-UL19-1368 + AUACACGGCCAUGCACUCCU 20 1608

HSV2-UL19-5320 + GAUACACGGCCAUGCACUCCU 21 9703

HSV2-UL19-5321 + CGAUACACGGCCAUGCACUCCU 22 9704

HSV2-UL19-5322 + CCGAUACACGGCCAUGCACUCCU 23 9705

HSV2-UL19-5323 + CCCGAUACACGGCCAUGCACUCCU 24 9706

HSV2-UL19-5324 + GAGCCUGCCCGCCCAGCU 18 9707

HSV2-UL19-5325 + UGAGCCUGCCCGCCCAGCU 19 9708

HSV2-UL19-2663 + CUGAGCCUGCCCGCCCAGCU 20 2781

HSV2-UL19-5326 + CCUGAGCCUGCCCGCCCAGCU 21 9709

HSV2-UL19-5327 + GCCUGAGCCUGCCCGCCCAGCU 22 9710

HSV2-UL19-5328 + GGCCUGAGCCUGCCCGCCCAGCU 23 9711

HSV2-UL19-5329 + CGGCCUGAGCCUGCCCGCCCAGCU 24 9712

HSV2-UL19-5330 + ACGCCUCCGACGCGCGCU 18 9713

HSV2-UL19-5331 + UACGCCUCCGACGCGCGCU 19 9714

HSV2-UL19-1218 + GUACGCCUCCGACGCGCGCU 20 1458

HSV2-UL19-5332 + AGUACGCCUCCGACGCGCGCU 21 9715

HSV2-UL19-5333 + AAGUACGCCUCCGACGCGCGCU 22 9716

HSV2-UL19-5334 + AAAGUACGCCUCCGACGCGCGCU 23 9717

HSV2-UL19-5335 + GAAAGUACGCCUCCGACGCGCGCU 24 9718

HSV2-UL19-5336 + GAACU UGAAGCAGGGGCU 18 9719

HSV2-UL19-5337 + AGAACUUGAAGCAGGGGCU 19 9720

HSV2-UL19-2529 + AAGAACUUGAAGCAGGGGCU 20 2692

HSV2-UL19-5338 + GAAGAACUUGAAGCAGGGGCU 21 9721

HSV2-UL19-5339 + UG AAGAACUUGAAGCAGGGGCU 22 9722

HSV2-UL19-5340 + GUGAAGAACUUGAAGCAGGGGCU 23 9723

HSV2-UL19-5341 + GGUGAAGAACUUGAAGCAGGGGCU 24 9724

HSV2-UL19-5342 + UGGUGUGGUCCAGGUGCU 18 9725

HSV2-UL19-5343 + AUGGUGUGGUCCAGGUGCU 19 9726

HSV2-UL19-1276 + GAUGGUGUGGUCCAGGUGCU 20 1516

HSV2-UL19-5344 + GG AUGGUGUGGUCCAGGUGCU 21 9727 HSV2-UL19-5345 + UGGAUGGUGUGGUCCAGGUGCU 22 9728

HSV2-UL19-5346 + UUGGAUGGUGUGGUCCAGGUGCU 23 9729

HSV2-UL19-5347 + UU UGGAUGGUGUGGUCCAGGUGCU 24

9730

HSV2-UL19-5348 + CCUCACCGGGGGCGAUCU 18 9731

HSV2-UL19-5349 + UCCUCACCGGGGGCGAUCU 19 9732

HSV2-UL19-2647 + CUCCUCACCGGGGGCGAUCU 20 2767

HSV2-UL19-5350 + ACUCCUCACCGGGGGCGAUCU 21 9733

HSV2-UL19-5351 + CACUCCUCACCGGGGGCGAUCU 22 9734

HSV2-UL19-5352 + GCACUCCUCACCGGGGGCGAUCU 23 9735

HSV2-UL19-5353 + GGCACUCCUCACCGGGGGCGAUCU 24 9736

HSV2-UL19-5354 + CGCGCACGCGCGUUGUCU 18 9737

HSV2-UL19-5355 + GCGCGCACGCGCGUUGUCU 19 9738

HSV2-UL19-1489 + CGCGCGCACGCGCGUUGUCU 20 1729

HSV2-UL19-5356 + CCGCGCGCACGCGCGUUGUCU 21 9739

HSV2-UL19-5357 + UCCGCGCGCACGCGCGUUGUCU 22 9740

HSV2-UL19-5358 + AUCCGCGCGCACGCGCGUUGUCU 23 9741

HSV2-UL19-5359 + GAUCCGCGCGCACGCGCGUUGUCU 24 9742

HSV2-UL19-5360 + UGUACAGCAGGUCCCCGU 18 9743

HSV2-UL19-5361 + UUGUACAGCAGGUCCCCGU 19 9744

HSV2-UL19-2533 + GUUGUACAGCAGGUCCCCGU 20 2696

HSV2-UL19-5362 + CGUUGUACAGCAGGUCCCCGU 21 9745

HSV2-UL19-5363 + CCGUUGUACAGCAGGUCCCCGU 22 9746

HSV2-UL19-5364 + CCCGUUGUACAGCAGGUCCCCGU 23 9747

HSV2-UL19-5365 + CCCCGUUGUACAGCAGGUCCCCGU 24 9748

HSV2-UL19-5366 + CGAGGUAGGUCACGAUGU 18 9749

HSV2-UL19-5367 + CCGAGGUAGGUCACGAUGU 19 9750

HSV2-UL19-2673 + CCCGAGGUAGGUCACGAUGU 20 2788

HSV2-UL19-5368 + CCCCGAGGUAGGUCACGAUGU 21 9751

HSV2-UL19-5369 + CCCCCGAGGUAGGUCACGAUGU 22 9752

HSV2-UL19-5370 + GCCCCCGAGGUAGGUCACGAUGU 23 9753

HSV2-UL19-5371 + CGCCCCCGAGGUAGGUCACGAUGU 24 9754

HSV2-UL19-5372 + CGCGCGCACGCGCGUUGU 18 9755

HSV2-UL19-5373 + CCGCGCGCACGCGCGUUGU 19 9756

HSV2-UL19-2752 + UCCGCGCGCACGCGCGUUGU 20 2844

HSV2-UL19-5374 + AUCCGCGCGCACGCGCGUUGU 21 9757

HSV2-UL19-5375 + GAUCCGCGCGCACGCGCGUUGU 22 9758

HSV2-UL19-5376 + AGAUCCGCGCGCACGCGCGU UGU 23 9759

HSV2-UL19-5377 + CAGAUCCGCGCGCACGCGCGUUGU 24 9760

HSV2-UL19-5378 + CU CG G U G U CCU CCACCU U 18 9761

HSV2-UL19-5379 + CCUCGGUGUCCUCCACCUU 19 9762

HSV2-UL19-2759 + GCCUCGGUGUCCUCCACCUU 20 2848

HSV2-UL19-5380 + GGCCUCGGUGUCCUCCACCUU 21 9763

HSV2-UL19-5381 + CGGCCUCGGUGUCCUCCACCUU 22 9764

HSV2-UL19-5382 + UCGGCCUCGGUGUCCUCCACCUU 23 9765

HSV2-UL19-5383 + GUCGGCCUCGGUGUCCUCCACCUU 24 9766 HSV2-UL19-5384 + GCGGCCGAGGUAAAAGUU 18 9767

HSV2-UL19-5385 + CGCGGCCGAGGUAAAAGUU 19 9768

HSV2-UL19-2561 + CCGCGGCCGAGGUAAAAGUU 20 2715

HSV2-UL19-5386 + CCCGCGGCCGAGGUAAAAGUU 21 9769

HSV2-UL19-5387 + CCCCGCGGCCGAGGUAAAAGUU 22 9770

HSV2-UL19-5388 + GCCCCGCGGCCGAGGUAAAAGUU 23 9771

HSV2-UL19-5389 + GGCCCCGCGGCCGAGGUAAAAGUU 24 9772

HSV2-UL19-5390 + GACGACGACGCGCCCGUU 18 9773

HSV2-UL19-5391 + CGACGACGACGCGCCCGUU 19 9774

HSV2-UL19-2633 + UCGACGACGACGCGCCCGUU 20 2759

HSV2-UL19-5392 + GUCGACGACGACGCGCCCGUU 21 9775

HSV2-UL19-5393 + CGUCGACGACGACGCGCCCGUU 22 9776

HSV2-UL19-5394 + CCGUCGACGACGACGCGCCCGUU 23 9777

HSV2-UL19-5395 + CCCGUCGACGACGACGCGCCCGUU 24 9778

HSV2-UL19-5396 + GCGCUGCCGCCAGGCGUU 18 9779

HSV2-UL19-5397 + GGCGCUGCCGCCAGGCGUU 19 9780

HSV2-UL19-2711 + AGGCGCUGCCGCCAGGCGU U 20 2818

HSV2-UL19-5398 + GAGGCGCUGCCGCCAGGCGU U 21 9781

HSV2-UL19-5399 + CGAGGCGCUGCCGCCAGGCGUU 22 9782

HSV2-UL19-5400 + GCG AGGCGCUGCCGCCAGGCGU U 23 9783

HSV2-UL19-5401 + GGCGAGGCGCUGCCGCCAGGCGUU 24 9784

HSV2-UL19-5402 + CAUGUCCGUAACGGGGUU 18 9785

HSV2-UL19-5403 + CCAUGUCCGUAACGGGGUU 19 9786

HSV2-UL19-2568 + CCCAUGUCCGUAACGGGGUU 20 2717

HSV2-UL19-5404 + GCCCAUGUCCGUAACGGGGUU 21 9787

HSV2-UL19-5405 + UGCCCAUGUCCGUAACGGGGUU 22 9788

HSV2-UL19-5406 + UUGCCCAUGUCCGUAACGGGGUU 23 9789

HSV2-UL19-5407 + GUUGCCCAUGUCCGUAACGGGGUU 24 9790

HSV2-UL19-5408 + CGGCCGAGGUAAAAGUUU 18 9791

HSV2-UL19-5409 + GCGGCCGAGGUAAAAGUUU 19 9792

HSV2-UL19-1196 + CGCGGCCGAGGUAAAAGUU U 20 1436

HSV2-UL19-5410 + CCGCGGCCGAGGUAAAAGUU U 21 9793

HSV2-UL19-5411 + CCCGCGGCCGAGGUAAAAGUU U 22 9794

HSV2-UL19-5412 + CCCCGCGGCCGAGGUAAAAGUU U 23 9795

HSV2-UL19-5413 + GCCCCGCGGCCGAGGUAAAAGUUU 24 9796

HSV2-UL19-5414 + GGCCGAGGUAAAAGUUUU 18 9797

HSV2-UL19-5415 + CGGCCGAGGUAAAAGUUUU 19 9798

HSV2-UL19-1197 + GCGGCCGAGGUAAAAGUUUU 20 1437

HSV2-UL19-5416 + CGCGGCCGAGGUAAAAGUU UU 21 9799

HSV2-UL19-5417 + CCGCGGCCGAGGUAAAAGUU UU 22 9800

HSV2-UL19-5418 + CCCGCGGCCGAGGUAAAAGUUUU 23 9801

HSV2-UL19-5419 + CCCCGCGGCCGAGGUAAAAGUUUU 24 9802

HSV2-UL19-5420 - CGGGGACCUGCUGUACAA 18 9803

HSV2-UL19-5421 - ACGGGGACCUGCUGUACAA 19 9804

HSV2-UL19-696 - UACGGGGACCUGCUGUACAA 20 936

HSV2-UL19-5422 - GU ACGGGGACCUGCUGUACAA 21 9805 HSV2-UL19-5423 - CGUACGGGGACCUGCUGUACAA 22 9806

HSV2-UL19-5424 - UCGUACGGGGACCUGCUGUACAA 23 9807

HSV2-UL19-5425 - CUCGUACGGGGACCUGCUGUACAA 24 9808

HSV2-UL19-5426 - CGGGGCCUAUCACCUCAA 18 9809

HSV2-UL19-5427 - ACGGGGCCUAUCACCUCAA 19 9810

HSV2-UL19-699 - AACGGGGCCUAUCACCUCAA 20 939

HSV2-UL19-5428 - CAACGGGGCCUAUCACCUCAA 21 9811

HSV2-UL19-5429 - ACAACGGGGCCUAUCACCUCAA 22 9812

HSV2-UL19-5430 - UACAACGGGGCCUAUCACCUCAA 23 9813

HSV2-UL19-5431 - GUACAACGGGGCCUAUCACCUCAA 24 9814

HSV2-UL19-5432 - CAGGUGGCCCGCCACGAA 18 9815

HSV2-UL19-5433 - CCAGGUGGCCCGCCACGAA 19 9816

HSV2-UL19-2183 - UCCAGGUGGCCCGCCACGAA 20 2471

HSV2-UL19-5434 - CUCCAGGUGGCCCGCCACGAA 21 9817

HSV2-UL19-5435 - GCUCCAGGUGGCCCGCCACGAA 22 9818

HSV2-UL19-5436 - AGCUCCAGGUGGCCCGCCACGAA 23 9819

HSV2-UL19-5437 - CAGCUCCAGGUGGCCCGCCACGAA 24 9820

HSV2-UL19-5438 - GGAGCAACUCGAGGCGAA 18 9821

HSV2-UL19-5439 - AGGAGCAACUCGAGGCGAA 19 9822

HSV2-UL19-2038 - GAGGAGCAACUCGAGGCGAA 20 2376

HSV2-UL19-5440 - GG AGGAGCAACUCGAGGCGAA 21 9823

HSV2-UL19-5441 - AGGAGGAGCAACUCGAGGCGAA 22 9824

HSV2-UL19-5442 - CAGG AGGAGCAACUCGAGGCGAA 23 9825

HSV2-UL19-5443 - GCAGGAGGAGCAACUCGAGGCGAA 24 9826

HSV2-UL19-5444 - G AGCG U CU CAU CG U G G AA 18 9827

HSV2-UL19-5445 - GGAGCGUCUCAUCGUGGAA 19 9828

HSV2-UL19-2235 - UGGAGCGUCUCAUCGUGGAA 20 2499

HSV2-UL19-5446 - CUGGAGCGUCUCAUCGUGGAA 21 9829

HSV2-UL19-5447 - CCUGGAGCGUCUCAUCGUGGAA 22 9830

HSV2-UL19-5448 - GCCUGGAGCGUCUCAUCGUGGAA 23 9831

HSV2-UL19-5449 - UGCCUGGAGCGUCUCAUCGUGGAA 24 9832

HSV2-UL19-5450 - CCUGGUCGCCGAGCUGAA 18 9833

HSV2-UL19-5451 - CCCUGGUCGCCGAGCUGAA 19 9834

HSV2-UL19-2006 - ACCCUGGUCGCCGAGCUGAA 20 2351

HSV2-UL19-5452 - GACCCUGGUCGCCGAGCUGAA 21 9835

HSV2-UL19-5453 - CGACCCUGGUCGCCGAGCUGAA 22 9836

HSV2-UL19-5454 - GCGACCCUGGUCGCCGAGCUGAA 23 9837

HSV2-UL19-5455 - GGCG ACCCUGGUCGCCGAGCUGAA 24 9838

HSV2-UL19-5456 - CGGCGAGAUGGUCUUGAA 18 9839

HSV2-UL19-5457 - ACGGCGAGAUGGUCUUGAA 19 9840

HSV2-UL19-386 - UACGGCGAGAUGGUCUUGAA 20 626

HSV2-UL19-5458 - CUACGGCGAGAUGGUCUUGAA 21 9841

HSV2-UL19-5459 - CCU ACGGCGAGAUGGUCUUGAA 22 9842

HSV2-UL19-5460 - ACCUACGGCGAGAUGGUCUUGAA 23 9843

HSV2-UL19-5461 - GACCUACGGCGAGAUGGUCUUGAA 24 9844

HSV2-UL19-5462 - UCAAGCGCAUGUUCCACA 18 9845 HSV2-UL19-5463 - GUCAAGCGCAUGUUCCACA 19 9846

HSV2-UL19-2149 - GGUCAAGCGCAUGUUCCACA 20 2446

HSV2-UL19-5464 - CGGUCAAGCGCAUGUUCCACA 21 9847

HSV2-UL19-5465 - ACGGUCAAGCGCAUGUUCCACA 22 9848

HSV2-UL19-5466 - CACGGUCAAGCGCAUGUUCCACA 23 9849

HSV2-UL19-5467 - ACACGGUCAAGCGCAUGUUCCACA 24 9850

HSV2-UL19-5468 - UGCAACGAUAUCUCGACA 18 9851

HSV2-UL19-5469 - AUGCAACGAUAUCUCGACA 19 9852

HSV2-UL19-2001 - CAUGCAACGAUAUCUCGACA 20 2346

HSV2-UL19-5470 - CCAUGCAACGAUAUCUCGACA 21 9853

HSV2-UL19-5471 - CCCAUGCAACGAUAUCUCGACA 22 9854

HSV2-UL19-5472 - GCCCAUGCAACGAUAUCUCGACA 23 9855

HSV2-UL19-5473 - UGCCCAUGCAACGAUAUCUCGACA 24 9856

HSV2-UL19-5474 - UCCUGAAGGUGGAGGACA 18 9857

HSV2-UL19-5475 - UUCCUGAAGGUGGAGGACA 19 9858

HSV2-UL19-2021 - CU UCCUGAAGGUGGAGGACA 20 2363

HSV2-UL19-5476 - CCU UCCUGAAGGUGGAGGACA 21 9859

HSV2-UL19-5477 - UCCU UCCUGAAGGUGGAGGACA 22 9860

HSV2-UL19-5478 - GUCCU UCCUGAAGGUGGAGGACA 23 9861

HSV2-UL19-5479 - AGUCCUUCCUGAAGGUGGAGGACA 24 9862

HSV2-UL19-5480 - GCGUGUACGCGGGGGACA 18 9863

HSV2-UL19-5481 - GGCGUGUACGCGGGGGACA 19 9864

HSV2-UL19-680 - CGGCGUGUACGCGGGGGACA 20 920

HSV2-UL19-5482 - GCGGCGUGUACGCGGGGGACA 21 9865

HSV2-UL19-5483 - GGCGGCGUGUACGCGGGGGACA 22 9866

HSV2-UL19-5484 - CGGCGGCGUGUACGCGGGGGACA 23 9867

HSV2-UL19-5485 - CCGGCGGCGUGUACGCGGGGGACA 24 9868

HSV2-UL19-5486 - ACGGGGACCUGCUGUACA 18 9869

HSV2-UL19-5487 - UACGGGGACCUGCUGUACA 19 9870

HSV2-UL19-2227 - GU ACGGGGACCUGCUGUACA 20 2495

HSV2-UL19-5488 - CGU ACGGGGACCUGCUGUACA 21 9871

HSV2-UL19-5489 - UCGUACGGGGACCUGCUGUACA 22 9872

HSV2-UL19-5490 - CUCGUACGGGGACCUGCUGUACA 23 9873

HSV2-UL19-5491 - UCUCGUACGGGGACCUGCUGUACA 24 9874

HSV2-UL19-5492 - CGCGGCCCUGGACCGCCA 18 9875

HSV2-UL19-5493 - ACGCGGCCCUGGACCGCCA 19 9876

HSV2-UL19-2127 - CACGCGGCCCUGGACCGCCA 20 2434

HSV2-UL19-5494 - ACACGCGGCCCUGGACCGCCA 21 9877

HSV2-UL19-5495 - GACACGCGGCCCUGGACCGCCA 22 9878

HSV2-UL19-5496 - CGACACGCGGCCCUGGACCGCCA 23 9879

HSV2-UL19-5497 - GCGACACGCGGCCCUGGACCGCCA 24 9880

HSV2-UL19-5498 - GCCAUCGCCGCGAGGCCA 18 9881

HSV2-UL19-5499 - GGCCAUCGCCGCGAGGCCA 19 9882

HSV2-UL19-2012 - GGGCCAUCGCCGCGAGGCCA 20 2356

HSV2-UL19-5500 - CGGGCCAUCGCCGCGAGGCCA 21 9883

HSV2-UL19-5501 - GCGGGCCAUCGCCGCGAGGCCA 22 9884 HSV2-UL19-5502 - GGCGGGCCAUCGCCGCGAGGCCA 23 9885

HSV2-UL19-5503 - AGGCGGGCCAUCGCCGCGAGGCCA 24 9886

HSV2-UL19-5504 - GGCUGUUCAACCCGGCCA 18 9887

HSV2-UL19-5505 - GGGCUGUUCAACCCGGCCA 19 9888

HSV2-UL19-422 - GGGGCUGU UCAACCCGGCCA 20 662

HSV2-UL19-5506 - UGGGGCUGUUCAACCCGGCCA 21 9889

HSV2-UL19-5507 - CUGGGGCUGUUCAACCCGGCCA 22 9890

HSV2-UL19-5508 - CCUGGGGCUGUUCAACCCGGCCA 23 9891

HSV2-UL19-5509 - CCCUGGGGCUGU UCAACCCGGCCA 24 9892

HSV2-UL19-5510 - AGGUUCUGCGGCUGUCCA 18 9893

HSV2-UL19-5511 - CAGGUUCUGCGGCUGUCCA 19 9894

HSV2-UL19-436 - CCAGGUUCUGCGGCUGUCCA 20 676

HSV2-UL19-5512 - ACCAGGUUCUGCGGCUGUCCA 21 9895

HSV2-UL19-5513 - CACCAGGUUCUGCGGCUGUCCA 22 9896

HSV2-UL19-5514 - CCACCAGGUUCUGCGGCUGUCCA 23 9897

HSV2-UL19-5515 - ACCACCAGGUUCUGCGGCUGUCCA 24 9898

HSV2-UL19-5516 - AAGCCGCGAUUCACGGCA 18 9899

HSV2-UL19-5517 - CAAGCCGCGAUUCACGGCA 19 9900

HSV2-UL19-2103 - G CAAG CCGCGAUU CACG G CA 20 2417

HSV2-UL19-5518 - UGCAAGCCGCGAUUCACGGCA 21 9901

HSV2-UL19-5519 - CUGCAAGCCGCGAUUCACGGCA 22 9902

HSV2-UL19-5520 - G CU G CAAG CCGCGAUUCACGGCA 23 9903

HSV2-UL19-5521 - UGCUGCAAGCCGCGAUUCACGGCA 24 9904

HSV2-UL19-5522 - ACGGGGCCUAUCACCUCA 18 9905

HSV2-UL19-5523 - AACGGGGCCUAUCACCUCA 19 9906

HSV2-UL19-2229 - CAACGGGGCCUAUCACCUCA 20 2496

HSV2-UL19-5524 - ACAACGGGGCCUAUCACCUCA 21 9907

HSV2-UL19-5525 - UACAACGGGGCCUAUCACCUCA 22 9908

HSV2-UL19-5526 - GUACAACGGGGCCUAUCACCUCA 23 9909

HSV2-UL19-5527 - UGUACAACGGGGCCUAUCACCUCA 24 9910

HSV2-UL19-5528 - GCCGCACCGGCCGGCCGA 18 9911

HSV2-UL19-5529 - GGCCGCACCGGCCGGCCGA 19 9912

HSV2-UL19-2135 - CGGCCGCACCGGCCGGCCGA 20 2438

HSV2-UL19-5530 - CCGGCCGCACCGGCCGGCCGA 21 9913

HSV2-UL19-5531 - ACCGGCCGCACCGGCCGGCCGA 22 9914

HSV2-UL19-5532 - GACCGGCCGCACCGGCCGGCCGA 23 9915

HSV2-UL19-5533 - CGACCGGCCGCACCGGCCGGCCGA 24 9916

HSV2-UL19-5534 - GCUACGCAGCGCCCGCGA 18 9917

HSV2-UL19-5535 - UGCUACGCAGCGCCCGCGA 19 9918

HSV2-UL19-717 - CUGCUACGCAGCGCCCGCGA 20 957

HSV2-UL19-5536 - CCUGCUACGCAGCGCCCGCGA 21 9919

HSV2-UL19-5537 - CCCUGCUACGCAGCGCCCGCGA 22 9920

HSV2-UL19-5538 - GCCCUGCUACGCAGCGCCCGCGA 23 9921

HSV2-UL19-5539 - CGCCCUGCUACGCAGCGCCCGCGA 24 9922

HSV2-UL19-5540 - CAACAUGCGCAUCUUCGA 18 9923

HSV2-UL19-5541 - CCAACAUGCGCAUCUUCGA 19 9924 HSV2-UL19-581 - GCCAACAU G CG CAU CU U CG A 20 821

HSV2-UL19-5542 - GGCCAACAUGCGCAUCUUCGA 21 9925

HSV2-UL19-5543 - CGGCCAACAUGCGCAUCUUCGA 22 9926

HSV2-UL19-5544 - ACGGCCAACAUGCGCAUCUUCGA 23 9927

HSV2-UL19-5545 - GACGGCCAACAUGCGCAUCUUCGA 24 9928

HSV2-UL19-5546 - GUACGCGGGGGACAAGGA 18 9929

HSV2-UL19-5547 - UGUACGCGGGGGACAAGGA 19 9930

HSV2-UL19-682 - GUGUACGCGGGGGACAAGGA 20 922

HSV2-UL19-5548 - CGUGUACGCGGGGGACAAGGA 21 9931

HSV2-UL19-5549 - GCG UGUACGCGGGGGACAAGGA 22 9932

HSV2-UL19-5550 - GGCGUGUACGCGGGGGACAAGGA 23 9933

HSV2-UL19-5551 - CGGCGUGUACGCGGGGGACAAGGA 24 9934

HSV2-UL19-5552 - CAGCUGUUUUUUUGGGGA 18 9935

HSV2-UL19-5553 - GCAGCUGUUUUUUUGGGGA 19 9936

HSV2-UL19-2065 - GGCAGCUGU UUUUUUGGGGA 20 2391

HSV2-UL19-5554 - CGGCAGCUGUUUUUUUGGGGA 21 9937

HSV2-UL19-5555 - CCGGCAGCUGUUUUUUUGGGGA 22 9938

HSV2-UL19-5556 - CCCGGCAGCUGUUUUUUUGGGGA 23 9939

HSV2-UL19-5557 - CCCCGGCAGCUGUUUUUUUGGGGA 24 9940

HSV2-UL19-5558 - GGCAGGACCGCUUCGUGA 18 9941

HSV2-UL19-5559 - CGGCAGGACCGCUUCGUGA 19 9942

HSV2-UL19-2176 - GCGGCAGGACCGCUUCGUGA 20 2465

HSV2-UL19-5560 - UGCGGCAGGACCGCU UCGUGA 21 9943

HSV2-UL19-5561 - GUGCGGCAGGACCGCUUCGUGA 22 9944

HSV2-UL19-5562 - CGUGCGGCAGGACCGCUUCGUGA 23 9945

HSV2-UL19-5563 - UCGUGCGGCAGGACCGCUUCGUGA 24 9946

HSV2-UL19-5564 - ACGGCGAGAUGGUCUUGA 18 9947

HSV2-UL19-5565 - UACGGCGAGAUGGUCUUGA 19 9948

HSV2-UL19-2024 - CU ACGGCGAGAUGGUCUUGA 20 2366

HSV2-UL19-5566 - CCUACGGCGAGAUGGUCUUGA 21 9949

HSV2-UL19-5567 - ACCU ACGGCGAGAUGGUCUUGA 22 9950

HSV2-UL19-5568 - GACCU ACGGCGAGAUGGUCUUGA 23 9951

HSV2-UL19-5569 - UGACCUACGGCGAGAUGGUCUUGA 24 9952

HSV2-UL19-5570 - UCAGUGCAUCACCAGCUA 18 9953

HSV2-UL19-5571 - CUCAGUGCAUCACCAGCUA 19 9954

HSV2-UL19-2104 - ACU CAG U GCAU CACCAG CU A 20 2418

HSV2-UL19-5572 - GACUCAGUGCAUCACCAGCUA 21 9955

HSV2-UL19-5573 - UGACUCAGUGCAUCACCAGCUA 22 9956

HSV2-UL19-5574 - GUGACUCAGUGCAUCACCAGCUA 23 9957

HSV2-UL19-5575 - CGUGACUCAGUGCAUCACCAGCUA 24 9958

HSV2-UL19-5576 - UACGACGCCUCCCCGCUA 18 9959

HSV2-UL19-5577 - CUACGACGCCUCCCCGCUA 19 9960

HSV2-UL19-2250 - UCUACGACGCCUCCCCGCUA 20 2507

HSV2-UL19-5578 - AUCUACGACGCCUCCCCGCUA 21 9961

HSV2-UL19-5579 - CAUCUACGACGCCUCCCCGCUA 22 9962

HSV2-UL19-5580 - UCAUCUACGACGCCUCCCCGCUA 23 9963 HSV2-UL19-5581 - CUCAUCUACGACGCCUCCCCGCUA 24 9964

HSV2-UL19-5582 - CGAGGCCGCGAAUCCGUA 18 9965

HSV2-UL19-5583 - UCGAGGCCGCGAAUCCGUA 19 9966

HSV2-UL19-446 - GUCGAGGCCGCGAAUCCGUA 20 686

HSV2-UL19-5584 - CG UCGAGGCCGCGAAUCCGUA 21 9967

HSV2-UL19-5585 - CCGUCGAGGCCGCGAAUCCGUA 22 9968

HSV2-UL19-5586 - CCCGUCGAGGCCGCGAAUCCGUA 23 9969

HSV2-UL19-5587 - CCCCGUCGAGGCCGCGAAUCCGUA 24 9970

HSV2-UL19-5588 - GUCGCAGCGGUUCUCGUA 18 9971

HSV2-UL19-5589 - CGUCGCAGCGGUUCUCGUA 19 9972

HSV2-UL19-693 - GCGUCGCAGCGGUUCUCGUA 20 933

HSV2-UL19-5590 - GGCGUCGCAGCGGU UCUCGUA 21 9973

HSV2-UL19-5591 - GGGCGUCGCAGCGGUUCUCGUA 22 9974

HSV2-UL19-5592 - UGGGCGUCGCAGCGGUUCUCGUA 23 9975

HSV2-UL19-5593 - GUGGGCGUCGCAGCGGUUCUCGUA 24 9976

HSV2-UL19-5594 - GGAGUGCAUGGCCGUGUA 18 9977

HSV2-UL19-5595 - AGGAGUGCAUGGCCGUGUA 19 9978

HSV2-UL19-2113 - GAGGAGUGCAUGGCCGUGUA 20 2423

HSV2-UL19-5596 - CGAGGAGUGCAUGGCCGUGUA 21 9979

HSV2-UL19-5597 - CCGAGGAGUGCAUGGCCGUGUA 22 9980

HSV2-UL19-5598 - CCCG AGGAGUGCAUGGCCGUGUA 23 9981

HSV2-UL19-5599 - CCCCGAGGAGUGCAUGGCCGUGUA 24 9982

HSV2-UL19-5600 - ACCCAGCCGCGCGGAAAC 18 9983

HSV2-UL19-5601 - CACCCAGCCGCGCGGAAAC 19 9984

HSV2-UL19-2191 - UCACCCAGCCGCGCGGAAAC 20 2474

HSV2-UL19-5602 - CUCACCCAGCCGCGCGGAAAC 21 9985

HSV2-UL19-5603 - GCUCACCCAGCCGCGCGGAAAC 22 9986

HSV2-UL19-5604 - CGCUCACCCAGCCGCGCGGAAAC 23 9987

HSV2-UL19-5605 - ACGCUCACCCAGCCGCGCGGAAAC 24 9988

HSV2-UL19-5606 - AUAUGCAGGAGGAGCAAC 18 9989

HSV2-UL19-5607 - GAUAUGCAGGAGGAGCAAC 19 9990

HSV2-UL19-2036 - GGAUAUGCAGGAGGAGCAAC 20 2374

HSV2-UL19-5608 - UGGAUAUGCAGGAGGAGCAAC 21 9991

HSV2-UL19-5609 - CUGGAUAUGCAGGAGGAGCAAC 22 9992

HSV2-UL19-5610 - GCUGGAUAUGCAGGAGGAGCAAC 23 9993

HSV2-UL19-5611 - UGCUGGAUAUGCAGGAGGAGCAAC 24 9994

HSV2-UL19-5612 - GAGCAACUCGAGGCGAAC 18 9995

HSV2-UL19-5613 - GGAGCAACUCGAGGCGAAC 19 9996

HSV2-UL19-402 - AGGAGCAACUCGAGGCGAAC 20 642

HSV2-UL19-5614 - GAGGAGCAACUCGAGGCGAAC 21 9997

HSV2-UL19-5615 - GGAGGAGCAACUCGAGGCGAAC 22 9998

HSV2-UL19-5616 - AGGAGGAGCAACUCGAGGCGAAC 23 9999

HSV2-UL19-5617 - CAGGAGGAGCAACUCGAGGCGAAC 24 10000

HSV2-UL19-5618 - GGGAGACGCUGGAUGAAC 18 10001

HSV2-UL19-5619 - CGGGAGACGCUGGAUGAAC 19 10002

HSV2-UL19-2043 - CCGGGAGACGCUGGAUGAAC 20 2379 HSV2-UL19-5620 - ACCGGGAGACGCUGGAUGAAC 21 10003

HSV2-UL19-5621 - AACCGGGAGACGCUGGAUGAAC 22 10004

HSV2-UL19-5622 - GAACCGGGAGACGCUGGAUGAAC 23 10005

HSV2-UL19-5623 - CGAACCGGGAGACGCUGGAUGAAC 24 10006

HSV2-UL19-5624 - GUGCCCGCCCCCGGCCAC 18 10007

HSV2-UL19-5625 - GGUGCCCGCCCCCGGCCAC 19 10008

HSV2-UL19-2059 - UGGUGCCCGCCCCCGGCCAC 20 2389

HSV2-UL19-5626 - CUGGUGCCCGCCCCCGGCCAC 21 10009

HSV2-UL19-5627 - CCUGGUGCCCGCCCCCGGCCAC 22 10010

HSV2-UL19-5628 - ACCUGGUGCCCGCCCCCGGCCAC 23 10011

HSV2-UL19-5629 - GACCUGGUGCCCGCCCCCGGCCAC 24 10012

HSV2-UL19-5630 - CUCAUGGCCCCCCAGCAC 18 10013

HSV2-UL19-5631 - GCUCAUGGCCCCCCAGCAC 19 10014

HSV2-UL19-2155 - UGCUCAUGGCCCCCCAGCAC 20 2451

HSV2-UL19-5632 - CUGCUCAUGGCCCCCCAGCAC 21 10015

HSV2-UL19-5633 - GCUGCUCAUGGCCCCCCAGCAC 22 10016

HSV2-UL19-5634 - UGCUGCUCAUGGCCCCCCAGCAC 23 10017

HSV2-UL19-5635 - GUGCUGCUCAUGGCCCCCCAGCAC 24 10018

HSV2-UL19-5636 - GUGCGGAGCCUGGACGAC 18 10019

HSV2-UL19-5637 - CGUGCGGAGCCUGGACGAC 19 10020

HSV2-UL19-2030 - CCGUGCGGAGCCUGGACGAC 20 2370

HSV2-UL19-5638 - GCCGUGCGGAGCCUGGACGAC 21 10021

HSV2-UL19-5639 - GGCCGUGCGGAGCCUGGACGAC 22 10022

HSV2-UL19-5640 - AGGCCGUGCGGAGCCUGGACGAC 23 10023

HSV2-UL19-5641 - AAGGCCGUGCGGAGCCUGGACGAC 24 10024

HSV2-UL19-5642 - UGCUCCGCGGCGCCCGAC 18 10025

HSV2-UL19-5643 - GUGCUCCGCGGCGCCCGAC 19 10026

HSV2-UL19-2152 - UGUGCUCCGCGGCGCCCGAC 20 2449

HSV2-UL19-5644 - CUGUGCUCCGCGGCGCCCGAC 21 10027

HSV2-UL19-5645 - GCUGUGCUCCGCGGCGCCCGAC 22 10028

HSV2-UL19-5646 - UGCUGUGCUCCGCGGCGCCCGAC 23 10029

HSV2-UL19-5647 - CUGCUGUGCUCCGCGGCGCCCGAC 24 10030

HSV2-UL19-5648 - AAGCGGAGCUUUUGCGAC 18 10031

HSV2-UL19-5649 - GAAGCGGAGCUUUUGCGAC 19 10032

HSV2-UL19-2008 - UGAAGCGGAGCUUUUGCGAC 20 2352

HSV2-UL19-5650 - CUGAAGCGGAGCUUUUGCGAC 21 10033

HSV2-UL19-5651 - GCUGAAGCGGAGCUUUUGCGAC 22 10034

HSV2-UL19-5652 - AGCUGAAGCGGAGCUUUUGCGAC 23 10035

HSV2-UL19-5653 - GAGCUGAAGCGGAGCUUUUGCGAC 24 10036

HSV2-UL19-5654 - CGCAACCCCGUUACGGAC 18 10037

HSV2-UL19-5655 - CCG CAACCCCG U U ACG G AC 19 10038

HSV2-UL19-2196 - UCCGCAACCCCGUUACGGAC 20 2479

HSV2-UL19-5656 - GUCCGCAACCCCGUUACGGAC 21 10039

HSV2-UL19-5657 - CG UCCGCAACCCCGU U ACGG AC 22 10040

HSV2-UL19-5658 - CCG U CCG CAACCCCG U U ACG G AC 23 10041

HSV2-UL19-5659 - ACCG UCCGCAACCCCGU UACGG AC 24 10042 HSV2-UL19-5660 - GGCGUGUACGCGGGGGAC 18 10043

HSV2-UL19-5661 - CGGCGUGUACGCGGGGGAC 19 10044

HSV2-UL19-2216 - GCGGCGUGUACGCGGGGGAC 20 2490

HSV2-UL19-5662 - GGCGGCGUGUACGCGGGGGAC 21 10045

HSV2-UL19-5663 - CGGCGGCGUGUACGCGGGGGAC 22 10046

HSV2-UL19-5664 - CCGGCGGCGUGUACGCGGGGGAC 23 10047

HSV2-UL19-5665 - GCCGGCGGCGUGUACGCGGGGGAC 24 10048

HSV2-UL19-5666 - CGAGACUGCCGGAUUGAC 18 10049

HSV2-UL19-5667 - CCGAGACUGCCGGAUUGAC 19 10050

HSV2-UL19-2129 - ACCGAGACUGCCGGAUUGAC 20 2436

HSV2-UL19-5668 - CACCGAGACUGCCGGAUUGAC 21 10051

HSV2-UL19-5669 - CCACCGAGACUGCCGGAUUGAC 22 10052

HSV2-UL19-5670 - GCCACCGAGACUGCCGGAUUGAC 23 10053

HSV2-UL19-5671 - CGCCACCGAGACUGCCGGAUUGAC 24 10054

HSV2-UL19-5672 - CAGUGCAUCACCAGCUAC 18 10055

HSV2-UL19-5673 - UCAGUGCAUCACCAGCUAC 19 10056

HSV2-UL19-500 - CU CAG U G CAU CACCAG CU AC 20 740

HSV2-UL19-5674 - ACUCAGUGCAUCACCAGCUAC 21 10057

HSV2-UL19-5675 - G ACU CAG U GCAU CACCAG CU AC 22 10058

HSV2-UL19-5676 - UGACU CAGUGCAUCACCAGCUAC 23 10059

HSV2-UL19-5677 - GUGACUCAGUGCAUCACCAGCUAC 24 10060

HSV2-UL19-5678 - UCGCAGCGGUUCUCGUAC 18 10061

HSV2-UL19-5679 - GUCGCAGCGGUUCUCGUAC 19 10062

HSV2-UL19-694 - CGUCGCAGCGGUUCUCGUAC 20 934

HSV2-UL19-5680 - GCGUCGCAGCGGU UCUCGUAC 21 10063

HSV2-UL19-5681 - GGCGUCGCAGCGGUUCUCGUAC 22 10064

HSV2-UL19-5682 - GGGCGUCGCAGCGGUUCUCGUAC 23 10065

HSV2-UL19-5683 - UGGGCGUCGCAGCGGUUCUCGUAC 24 10066

HSV2-UL19-5684 - GCGGCCGGCGGCGUGUAC 18 10067

HSV2-UL19-5685 - CGCGGCCGGCGGCGUGUAC 19 10068

HSV2-UL19-2212 - GCGCGGCCGGCGGCGUGUAC 20 2489

HSV2-UL19-5686 - CGCGCGGCCGGCGGCGUGUAC 21 10069

HSV2-UL19-5687 - ACGCGCGGCCGGCGGCGUGUAC 22 10070

HSV2-UL19-5688 - GACGCGCGGCCGGCGGCGUGUAC 23 10071

HSV2-UL19-5689 - GGACGCGCGGCCGGCGGCGUGUAC 24 10072

HSV2-UL19-5690 - AGCAACUCGAGGCGAACC 18 10073

HSV2-UL19-5691 - GAGCAACUCGAGGCGAACC 19 10074

HSV2-UL19-403 - GG AGCAACUCGAGGCGAACC 20 643

HSV2-UL19-5692 - AGG AGCAACUCGAGGCGAACC 21 10075

HSV2-UL19-5693 - GAGGAGCAACUCGAGGCGAACC 22 10076

HSV2-UL19-5694 - GGAGG AGCAACUCGAGGCGAACC 23 10077

HSV2-UL19-5695 - AGGAGGAGCAACUCGAGGCGAACC 24 10078

HSV2-UL19-5696 - UGCCCGCCCCCGGCCACC 18 10079

HSV2-UL19-5697 - GUGCCCGCCCCCGGCCACC 19 10080

HSV2-UL19-428 - GGUGCCCGCCCCCGGCCACC 20 668

HSV2-UL19-5698 - UGGUGCCCGCCCCCGGCCACC 21 10081 HSV2-UL19-5699 - CUGGUGCCCGCCCCCGGCCACC 22 10082

HSV2-UL19-5700 - CCUGGUGCCCGCCCCCGGCCACC 23 10083

HSV2-UL19-5701 - ACCUGGUGCCCGCCCCCGGCCACC 24 10084

HSV2-UL19-5702 - CGGGGGCGCUGCUGCACC 18 10085

HSV2-UL19-5703 - GCGGGGGCGCUGCUGCACC 19 10086

HSV2-UL19-2139 - CGCGGGGGCGCUGCUGCACC 20 2440

HSV2-UL19-5704 - UCGCGGGGGCGCUGCUGCACC 21 10087

HSV2-UL19-5705 - CUCGCGGGGGCGCUGCUGCACC 22 10088

HSV2-UL19-5706 - UCUCGCGGGGGCGCUGCUGCACC 23 10089

HSV2-UL19-5707 - UUCUCGCGGGGGCGCUGCUGCACC 24 10090

HSV2-UL19-5708 - GGCGCGCCGGCAUGGACC 18 10091

HSV2-UL19-5709 - CGGCGCGCCGGCAUGGACC 19 10092

HSV2-UL19-2205 - GCGGCGCGCCGGCAUGGACC 20 2484

HSV2-UL19-5710 - CGCGGCGCGCCGGCAUGGACC 21 10093

HSV2-UL19-5711 - CCGCGGCGCGCCGGCAUGGACC 22 10094

HSV2-UL19-5712 - GCCGCGGCGCGCCGGCAUGGACC 23 10095

HSV2-UL19-5713 - UGCCGCGGCGCGCCGGCAUGGACC 24 10096

HSV2-UL19-5714 - GCCGAGUGCCAGAUGACC 18 10097

HSV2-UL19-5715 - CGCCGAGUGCCAGAUGACC 19 10098

HSV2-UL19-2085 - UCGCCGAGUGCCAGAUGACC 20 2406

HSV2-UL19-5716 - GUCGCCGAGUGCCAGAUGACC 21 10099

HSV2-UL19-5717 - GGUCGCCGAGUGCCAGAUGACC 22 10100

HSV2-UL19-5718 - GGGU CGCCGAGUGCCAGAUGACC 23 10101

HSV2-UL19-5719 - UGGGUCGCCGAGUGCCAGAUGACC 24 10102

HSV2-UL19-5720 - CAGGACCGCUUCGUGACC 18 10103

HSV2-UL19-5721 - GCAGGACCGCUUCGUGACC 19 10104

HSV2-UL19-2177 - GGCAGGACCGCUUCGUGACC 20 2466

HSV2-UL19-5722 - CGGCAGGACCGCUUCGUGACC 21 10105

HSV2-UL19-5723 - GCGGCAGGACCGCUUCGUGACC 22 10106

HSV2-UL19-5724 - UGCGGCAGGACCGCU UCGUGACC 23 10107

HSV2-UL19-5725 - GUGCGGCAGGACCGCUUCGUGACC 24 10108

HSV2-UL19-5726 - CGUACAUCGUGACCUACC 18 10109

HSV2-UL19-5727 - UCGUACAUCGUGACCUACC 19 10110

HSV2-UL19-2107 - CUCGUACAUCGUGACCUACC 20 2420

HSV2-UL19-5728 - UCUCGUACAUCGUGACCUACC 21 10111

HSV2-UL19-5729 - GUCUCGUACAUCGUGACCUACC 22 10112

HSV2-UL19-5730 - GGUCUCGUACAUCGUGACCUACC 23 10113

HSV2-UL19-5731 - UGGUCUCGUACAUCGUGACCUACC 24 10114

HSV2-UL19-5732 - CGCGGCCACGGCCAACCC 18 10115

HSV2-UL19-5733 - UCGCGGCCACGGCCAACCC 19 10116

HSV2-UL19-2223 - UUCGCGGCCACGGCCAACCC 20 2493

HSV2-UL19-5734 - CUUCGCGGCCACGGCCAACCC 21 10117

HSV2-UL19-5735 - CCUUCGCGGCCACGGCCAACCC 22 10118

HSV2-UL19-5736 - CCCUUCGCGGCCACGGCCAACCC 23 10119

HSV2-UL19-5737 - GCCCUUCGCGGCCACGGCCAACCC 24 10120

HSV2-UL19-5738 - UCGCCGGCCCUGCAACCC 18 10121 HSV2-UL19-5739 - UUCGCCGGCCCUGCAACCC 19 10122

HSV2-UL19-2209 - UU UCGCCGGCCCUGCAACCC 20 2488

HSV2-UL19-5740 - CUUUCGCCGGCCCUGCAACCC 21 10123

HSV2-UL19-5741 - ACUUUCGCCGGCCCUGCAACCC 22 10124

HSV2-UL19-5742 - UACUUUCGCCGGCCCUGCAACCC 23 10125

HSV2-UL19-5743 - CUACUU UCGCCGGCCCUGCAACCC 24 10126

HSV2-UL19-5744 - UGACGUUCGUCCUGCCCC 18 10127

HSV2-UL19-5745 - CUGACGUUCGUCCUGCCCC 19 10128

HSV2-UL19-418 - CCUGACGUUCGUCCUGCCCC 20 658

HSV2-UL19-5746 - ACCUGACGUUCGUCCUGCCCC 21 10129

HSV2-UL19-5747 - GACCUGACGUUCGUCCUGCCCC 22 10130

HSV2-UL19-5748 - GGACCUGACGUUCGUCCUGCCCC 23 10131

HSV2-UL19-5749 - UGGACCUGACGUUCGUCCUGCCCC 24 10132

HSV2-UL19-5750 - CUCGGGGGCGACCUCCCC 18 10133

HSV2-UL19-5751 - CCUCGGGGGCGACCUCCCC 19 10134

HSV2-UL19-2111 - ACCUCGGGGGCGACCUCCCC 20 2422

HSV2-UL19-5752 - UACCUCGGGGGCGACCUCCCC 21 10135

HSV2-UL19-5753 - CUACCUCGGGGGCGACCUCCCC 22 10136

HSV2-UL19-5754 - CCUACCUCGGGGGCGACCUCCCC 23 10137

HSV2-UL19-5755 - ACCUACCUCGGGGGCGACCUCCCC 24 10138

HSV2-UL19-5756 - CCGUGGUGCAGCACGCCC 18 10139

HSV2-UL19-5757 - CCCGUGGUGCAGCACGCCC 19 10140

HSV2-UL19-2161 - GCCCGUGGUGCAGCACGCCC 20 2455

HSV2-UL19-5758 - AGCCCGUGGUGCAGCACGCCC 21 10141

HSV2-UL19-5759 - CAGCCCGUGGUGCAGCACGCCC 22 10142

HSV2-UL19-5760 - CCAGCCCGUGGUGCAGCACGCCC 23 10143

HSV2-UL19-5761 - GCCAGCCCGUGGUGCAGCACGCCC 24 10144

HSV2-UL19-5762 - UGGUCUUCCUGGAGGCCC 18 10145

HSV2-UL19-5763 - CUGGUCUUCCUGGAGGCCC 19 10146

HSV2-UL19-413 - GCUGGUCUUCCUGGAGGCCC 20 653

HSV2-UL19-5764 - GGCUGGUCUUCCUGGAGGCCC 21 10147

HSV2-UL19-5765 - AGGCUGGUCUUCCUGGAGGCCC 22 10148

HSV2-UL19-5766 - CAGGCUGGUCUUCCUGGAGGCCC 23 10149

HSV2-UL19-5767 - ACAGGCUGGUCUUCCUGGAGGCCC 24 10150

HSV2-UL19-5768 - CCCUGGUCCCCCCGGCCC 18 10151

HSV2-UL19-5769 - CCCCUGGUCCCCCCGGCCC 19 10152

HSV2-UL19-595 - CCCCCUGGUCCCCCCGGCCC 20 835

HSV2-UL19-5770 - UCCCCCUGGUCCCCCCGGCCC 21 10153

HSV2-UL19-5771 - GUCCCCCUGGUCCCCCCGGCCC 22 10154

HSV2-UL19-5772 - CGUCCCCCUGGUCCCCCCGGCCC 23 10155

HSV2-UL19-5773 - ACGUCCCCCUGGUCCCCCCGGCCC 24 10156

HSV2-UL19-5774 - CUGACGUUCGUCCUGCCC 18 10157

HSV2-UL19-5775 - CCUGACGUUCGUCCUGCCC 19 10158

HSV2-UL19-2052 - ACCUGACGUUCGUCCUGCCC 20 2386

HSV2-UL19-5776 - GACCUGACGUUCGUCCUGCCC 21 10159

HSV2-UL19-5777 - GGACCUGACGUUCGUCCUGCCC 22 10160 HSV2-UL19-5778 - UGGACCUGACGUUCGUCCUGCCC 23 10161

HSV2-UL19-5779 - AUGGACCUGACGUUCGUCCUGCCC 24 10162

HSV2-UL19-5780 - CGCUUCGCCGCGCACGCC 18 10163

HSV2-UL19-5781 - GCGCUUCGCCGCGCACGCC 19 10164

HSV2-UL19-424 - AGCGCUUCGCCGCGCACGCC 20 664

HSV2-UL19-5782 - GAGCGCUUCGCCGCGCACGCC 21 10165

HSV2-UL19-5783 - GGAGCGCUUCGCCGCGCACGCC 22 10166

HSV2-UL19-5784 - UGGAGCGCU UCGCCGCGCACGCC 23 10167

HSV2-UL19-5785 - AUGGAGCGCUUCGCCGCGCACGCC 24 10168

HSV2-UL19-5786 - GUGGCGU UUCGUGACGCC 18 10169

HSV2-UL19-5787 - GGUGGCGUUUCGUGACGCC 19 10170

HSV2-UL19-483 - CGGUGGCGUUUCGUGACGCC 20 723

HSV2-UL19-5788 - CCGGUGGCGUU UCGUGACGCC 21 10171

HSV2-UL19-5789 - UCCGGUGGCGUUUCGUGACGCC 22 10172

HSV2-UL19-5790 - GUCCGGUGGCGUUUCGUGACGCC 23 10173

HSV2-UL19-5791 - UGUCCGGUGGCGUUUCGUGACGCC 24 10174

HSV2-UL19-5792 - AGCUCCAGGUGGCCCGCC 18 10175

HSV2-UL19-5793 - CAGCUCCAGGUGGCCCGCC 19 10176

HSV2-UL19-2182 - CCAGCUCCAGGUGGCCCGCC 20 2470

HSV2-UL19-5794 - GCCAGCUCCAGGUGGCCCGCC 21 10177

HSV2-UL19-5795 - GGCCAGCUCCAGGUGGCCCGCC 22 10178

HSV2-UL19-5796 - GGGCCAGCUCCAGGUGGCCCGCC 23 10179

HSV2-UL19-5797 - UGGGCCAGCUCCAGGUGGCCCGCC 24 10180

HSV2-UL19-5798 - UACCCCCUGGUGGGCGCC 18 10181

HSV2-UL19-5799 - CUACCCCCUGGUGGGCGCC 19 10182

HSV2-UL19-2051 - CCUACCCCCUGGUGGGCGCC 20 2385

HSV2-UL19-5800 - CCCUACCCCCUGGUGGGCGCC 21 10183

HSV2-UL19-5801 - GCCCUACCCCCUGGUGGGCGCC 22 10184

HSV2-UL19-5802 - UGCCCUACCCCCUGGUGGGCGCC 23 10185

HSV2-UL19-5803 - GUGCCCUACCCCCUGGUGGGCGCC 24 10186

HSV2-UL19-5804 - GCAAGGCGGGCCAUCGCC 18 10187

HSV2-UL19-5805 - GGCAAGGCGGGCCAUCGCC 19 10188

HSV2-UL19-2011 - GGGCAAGGCGGGCCAUCGCC 20 2355

HSV2-UL19-5806 - UGGGCAAGGCGGGCCAUCGCC 21 10189

HSV2-UL19-5807 - CUGGGCAAGGCGGGCCAUCGCC 22 10190

HSV2-UL19-5808 - CCUGGGCAAGGCGGGCCAUCGCC 23 10191

HSV2-UL19-5809 - UCCUGGGCAAGGCGGGCCAUCGCC 24 10192

HSV2-UL19-5810 - CUGGUCUUCCUGGAGGCC 18 10193

HSV2-UL19-5811 - GCUGGUCUUCCUGGAGGCC 19 10194

HSV2-UL19-2047 - GGCUGGUCUUCCUGGAGGCC 20 2382

HSV2-UL19-5812 - AGGCUGGUCUUCCUGGAGGCC 21 10195

HSV2-UL19-5813 - CAGGCUGGUCUUCCUGGAGGCC 22 10196

HSV2-UL19-5814 - ACAGGCUGGUCUUCCUGGAGGCC 23 10197

HSV2-UL19-5815 - GACAGGCUGGUCUUCCUGGAGGCC 24 10198

HSV2-UL19-5816 - GGGCUGUUCAACCCGGCC 18 10199

HSV2-UL19-5817 - GGGGCUGUUCAACCCGGCC 19 10200 HSV2-UL19-2054 - UGGGGCUGUUCAACCCGGCC 20 2387

HSV2-UL19-5818 - CUGGGGCUGUUCAACCCGGCC 21 10201

HSV2-UL19-5819 - CCUGGGGCUGU UCAACCCGGCC 22 10202

HSV2-UL19-5820 - CCCUGGGGCUGUUCAACCCGGCC 23 10203

HSV2-UL19-5821 - CCCCUGGGGCUGUUCAACCCGGCC 24 10204

HSV2-UL19-5822 - CCCCUGGUCCCCCCGGCC 18 10205

HSV2-UL19-5823 - CCCCCUGGUCCCCCCGGCC 19 10206

HSV2-UL19-2158 - UCCCCCUGGUCCCCCCGGCC 20 2454

HSV2-UL19-5824 - GUCCCCCUGGUCCCCCCGGCC 21 10207

HSV2-UL19-5825 - CGUCCCCCUGGUCCCCCCGGCC 22 10208

HSV2-UL19-5826 - ACGUCCCCCUGGUCCCCCCGGCC 23 10209

HSV2-UL19-5827 - GACGUCCCCCUGGUCCCCCCGGCC 24 10210

HSV2-UL19-5828 - CUUAUGCGACACGCGGCC 18 10211

HSV2-UL19-5829 - CCUUAUGCGACACGCGGCC 19 10212

HSV2-UL19-2126 - GCCUUAUGCGACACGCGGCC 20 2433

HSV2-UL19-5830 - GGCCUUAUGCGACACGCGGCC 21 10213

HSV2-UL19-5831 - CGGCCUUAUGCGACACGCGGCC 22 10214

HSV2-UL19-5832 - ACGGCCUUAUGCGACACGCGGCC 23 10215

HSV2-UL19-5833 - GACGGCCUUAUGCGACACGCGGCC 24 10216

HSV2-UL19-5834 - UACACCGCCGUCGCGGCC 18 10217

HSV2-UL19-5835 - CUACACCGCCGUCGCGGCC 19 10218

HSV2-UL19-2194 - GCUACACCGCCGUCGCGGCC 20 2477

HSV2-UL19-5836 - GGCUACACCGCCGUCGCGGCC 21 10219

HSV2-UL19-5837 - GGGCUACACCGCCGUCGCGGCC 22 10220

HSV2-UL19-5838 - UGGGCUACACCGCCGUCGCGGCC 23 10221

HSV2-UL19-5839 - GUGGGCUACACCGCCGUCGCGGCC 24 10222

HSV2-UL19-5840 - AAAACU U U UACCUCGGCC 18 10223

HSV2-UL19-5841 - CAAAACUUUUACCUCGGCC 19 10224

HSV2-UL19-2197 - CCAAAACUUUUACCUCGGCC 20 2480

HSV2-UL19-5842 - CCCAAAACUUUUACCUCGGCC 21 10225

HSV2-UL19-5843 - CCCCAAAACU U U UACCUCGGCC 22 10226

HSV2-UL19-5844 - CCCCCAAAACU U U U ACCU CG GCC 23 10227

HSV2-UL19-5845 - U CCCCCAAAACU U U U ACCUCG GCC 24 10228

HSV2-UL19-5846 - ACUUUACCCUGCCGGGCC 18 10229

HSV2-UL19-5847 - GACUU UACCCUGCCGGGCC 19 10230

HSV2-UL19-519 - CGACUUUACCCUGCCGGGCC 20 759

HSV2-UL19-5848 - ACGACUU UACCCUGCCGGGCC 21 10231

HSV2-UL19-5849 - GACGACUUUACCCUGCCGGGCC 22 10232

HSV2-UL19-5850 - GGACGACU UUACCCUGCCGGGCC 23 10233

HSV2-UL19-5851 - UGGACGACUUUACCCUGCCGGGCC 24 10234

HSV2-UL19-5852 - UUCGUGACGCCCGGGGCC 18 10235

HSV2-UL19-5853 - UUUCGUGACGCCCGGGGCC 19 10236

HSV2-UL19-486 - GUUUCGUGACGCCCGGGGCC 20 726

HSV2-UL19-5854 - CGUUUCGUGACGCCCGGGGCC 21 10237

HSV2-UL19-5855 - GCGUUUCGUGACGCCCGGGGCC 22 10238

HSV2-UL19-5856 - GGCGUUUCGUGACGCCCGGGGCC 23 10239 HSV2-UL19-5857 - UGGCGUUUCGUGACGCCCGGGGCC 24 10240

HSV2-UL19-5858 - AUCGCCACCCCCGUGGCC 18 10241

HSV2-UL19-5859 - CAUCGCCACCCCCGUGGCC 19 10242

HSV2-UL19-2208 - UCAUCGCCACCCCCGUGGCC 20 2487

HSV2-UL19-5860 - UUCAUCGCCACCCCCGUGGCC 21 10243

HSV2-UL19-5861 - GUUCAUCGCCACCCCCGUGGCC 22 10244

HSV2-UL19-5862 - AGUUCAUCGCCACCCCCGUGGCC 23 10245

HSV2-UL19-5863 - GAGU UCAUCGCCACCCCCGUGGCC 24 10246

HSV2-UL19-5864 - CGGCCAAACAUCGCUGCC 18 10247

HSV2-UL19-5865 - ACGGCCAAACAUCGCUGCC 19 10248

HSV2-UL19-704 - CACGGCCAAACAUCGCUGCC 20 944

HSV2-UL19-5866 - UCACGGCCAAACAUCGCUGCC 21 10249

HSV2-UL19-5867 - AUCACGGCCAAACAUCGCUGCC 22 10250

HSV2-UL19-5868 - CAUCACGGCCAAACAUCGCUGCC 23 10251

HSV2-UL19-5869 - ACAUCACGGCCAAACAUCGCUGCC 24 10252

HSV2-UL19-5870 - AGCGCCCGCCGGGGUGCC 18 10253

HSV2-UL19-5871 - AAGCGCCCGCCGGGGUGCC 19 10254

HSV2-UL19-2239 - UAAGCGCCCGCCGGGGUGCC 20 2501

HSV2-UL19-5872 - UUAAGCGCCCGCCGGGGUGCC 21 10255

HSV2-UL19-5873 - UU UAAGCGCCCGCCGGGGUGCC 22 10256

HSV2-UL19-5874 - GUUUAAGCGCCCGCCGGGGUGCC 23 10257

HSV2-UL19-5875 - AGUUUAAGCGCCCGCCGGGGUGCC 24 10258

HSV2-UL19-5876 - ACCUCGGGGGCGACCUCC 18 10259

HSV2-UL19-5877 - UACCUCGGGGGCGACCUCC 19 10260

HSV2-UL19-2110 - CUACCUCGGGGGCGACCUCC 20 2421

HSV2-UL19-5878 - CCUACCUCGGGGGCGACCUCC 21 10261

HSV2-UL19-5879 - ACCUACCUCGGGGGCGACCUCC 22 10262

HSV2-UL19-5880 - GACCUACCUCGGGGGCGACCUCC 23 10263

HSV2-UL19-5881 - UGACCUACCUCGGGGGCGACCUCC 24 10264

HSV2-UL19-5882 - CUACCAUAGCCGCCGUCC 18 10265

HSV2-UL19-5883 - GCUACCAUAGCCGCCGUCC 19 10266

HSV2-UL19-2099 - GGCUACCAUAGCCGCCGUCC 20 2414

HSV2-UL19-5884 - CGGCUACCAUAGCCGCCGUCC 21 10267

HSV2-UL19-5885 - CCGGCUACCAUAGCCGCCGUCC 22 10268

HSV2-UL19-5886 - GCCGGCUACCAUAGCCGCCGUCC 23 10269

HSV2-UL19-5887 - CGCCGGCUACCAUAGCCGCCGUCC 24 10270

HSV2-UL19-3494 - GCAACGUCCAGGCCGUCC 18 7877

HSV2-UL19-3495 - CGCAACGUCCAGGCCGUCC 19 7878

HSV2-UL19-3092 - GCGCAACGUCCAGGCCGUCC 20 7475

HSV2-UL19-3496 - CGCGCAACGUCCAGGCCGUCC 21 7879

HSV2-UL19-3497 - GCGCGCAACGUCCAGGCCGUCC 22 7880

HSV2-UL19-3498 - CGCGCGCAACGUCCAGGCCGUCC 23 7881

HSV2-UL19-3499 - UCGCGCGCAACGUCCAGGCCGUCC 24 7882

HSV2-UL19-5888 - AGUCCCCCCGGCCGGUCC 18 10271

HSV2-UL19-5889 - AAGUCCCCCCGGCCGGUCC 19 10272

HSV2-UL19-473 - GAAGUCCCCCCGGCCGGUCC 20 713 HSV2-UL19-5890 - CGAAGU CCCCCCGGCCGG UCC 21 10273

HSV2-UL19-5891 - GCGAAGUCCCCCCGGCCGGUCC 22 10274

HSV2-UL19-5892 - GGCGAAGUCCCCCCGGCCGGUCC 23 10275

HSV2-UL19-5893 - CGGCGAAGUCCCCCCGGCCGGUCC 24 10276

HSV2-UL19-5894 - UGCAGAACAUGGUGGUCC 18 10277

HSV2-UL19-5895 - CUGCAGAACAUGGUGGUCC 19 10278

HSV2-UL19-563 - GCUGCAGAACAUGGUGGUCC 20 803

HSV2-UL19-5896 - CGCUGCAGAACAUGGUGGUCC 21 10279

HSV2-UL19-5897 - ACGCUGCAGAACAUGGUGGUCC 22 10280

HSV2-UL19-5898 - CACGCUGCAGAACAUGGUGGUCC 23 10281

HSV2-UL19-5899 - CCACGCUGCAGAACAUGGUGGUCC 24 10282

HSV2-UL19-5900 - CAGGUUCUGCGGCUGUCC 18 10283

HSV2-UL19-5901 - CCAGGUUCUGCGGCUGUCC 19 10284

HSV2-UL19-2066 - ACCAGGUUCUGCGGCUGUCC 20 2392

HSV2-UL19-5902 - CACCAGGUUCUGCGGCUGUCC 21 10285

HSV2-UL19-5903 - CCACCAGGUUCUGCGGCUGUCC 22 10286

HSV2-UL19-5904 - ACCACCAGGUUCUGCGGCUGUCC 23 10287

HSV2-UL19-5905 - GACCACCAGGUUCUGCGGCUGUCC 24 10288

HSV2-UL19-5906 - GCGACAGGCUGGUCUUCC 18 10289

HSV2-UL19-5907 - GGCGACAGGCUGGUCUUCC 19 10290

HSV2-UL19-411 - AGGCGACAGGCUGGUCUUCC 20 651

HSV2-UL19-5908 - UAGGCGACAGGCUGGUCUUCC 21 10291

HSV2-UL19-5909 - AUAGGCGACAGGCUGGUCUUCC 22 10292

HSV2-UL19-5910 - CAUAGGCGACAGGCUGGUCUUCC 23 10293

HSV2-UL19-5911 - CCAUAGGCGACAGGCUGGUCUUCC 24 10294

HSV2-UL19-5912 - GGCAAGGCCGUGCGGAGC 18 10295

HSV2-UL19-5913 - GGGCAAGGCCGUGCGGAGC 19 10296

HSV2-UL19-2029 - UGGGCAAGGCCGUGCGGAGC 20 2369

HSV2-UL19-5914 - AUGGGCAAGGCCGUGCGGAGC 21 10297

HSV2-UL19-5915 - GAUGGGCAAGGCCGUGCGGAGC 22 10298

HSV2-UL19-5916 - UGAUGGGCAAGGCCGUGCGGAGC 23 10299

HSV2-UL19-5917 - GUGAUGGGCAAGGCCGUGCGGAGC 24 10300

HSV2-UL19-5918 - UGACGCACGCCGACACGC 18 10301

HSV2-UL19-5919 - CUGACGCACGCCGACACGC 19 10302

HSV2-UL19-2014 - CCUGACGCACGCCGACACGC 20 2358

HSV2-UL19-5920 - GCCUGACGCACGCCGACACGC 21 10303

HSV2-UL19-5921 - CGCCUGACGCACGCCGACACGC 22 10304

HSV2-UL19-5922 - CCGCCUGACGCACGCCGACACGC 23 10305

HSV2-UL19-5923 - CCCGCCU G ACG CACG CCG ACACGC 24 10306

HSV2-UL19-5924 - CGAUGGGGAGGCCCACGC 18 10307

HSV2-UL19-5925 - GCGAUGGGGAGGCCCACGC 19 10308

HSV2-UL19-2248 - CGCGAUGGGGAGGCCCACGC 20 2505

HSV2-UL19-5926 - CCGCGAUGGGGAGGCCCACGC 21 10309

HSV2-UL19-5927 - CCCGCGAUGGGGAGGCCCACGC 22 10310

HSV2-UL19-5928 - GCCCGCGAUGGGGAGGCCCACGC 23 10311

HSV2-UL19-5929 - CGCCCGCGAUGGGGAGGCCCACGC 24 10312 HSV2-UL19-5930 - GCGCUUCGCCGCGCACGC 18 10313

HSV2-UL19-5931 - AGCGCUUCGCCGCGCACGC 19 10314

HSV2-UL19-423 - GAGCGCUUCGCCGCGCACGC 20 663

HSV2-UL19-5932 - GGAGCGCUUCGCCGCGCACGC 21 10315

HSV2-UL19-5933 - UGGAGCGCUUCGCCGCGCACGC 22 10316

HSV2-UL19-5934 - AUGGAGCGCUUCGCCGCGCACGC 23 10317

HSV2-UL19-5935 - CAUGGAGCGCUUCGCCGCGCACGC 24 10318

HSV2-UL19-5936 - GGUGGCGUUUCGUGACGC 18 10319

HSV2-UL19-5937 - CGGUGGCGUUUCGUGACGC 19 10320

HSV2-UL19-2095 - CCGGUGGCGUUUCGUGACGC 20 2412

HSV2-UL19-5938 - UCCGGUGGCGUUUCGUGACGC 21 10321

HSV2-UL19-5939 - GUCCGGUGGCGUUUCGUGACGC 22 10322

HSV2-UL19-5940 - UGUCCGGUGGCGUUUCGUGACGC 23 10323

HSV2-UL19-5941 - GUGUCCGGUGGCGUUUCGUGACGC 24 10324

HSV2-UL19-5942 - AGACUGCCGGAUUGACGC 18 10325

HSV2-UL19-5943 - GAGACUGCCGGAUUGACGC 19 10326

HSV2-UL19-534 - CGAGACUGCCGGAUUGACGC 20 774

HSV2-UL19-5944 - CCG AGACUGCCGGAUUGACGC 21 10327

HSV2-UL19-5945 - ACCGAGACUGCCGGAUUGACGC 22 10328

HSV2-UL19-5946 - CACCGAGACUGCCGGAUUGACGC 23 10329

HSV2-UL19-5947 - CCACCGAGACUGCCGGAUUGACGC 24 10330

HSV2-UL19-5948 - GGCCGGCGGCGUGUACGC 18 10331

HSV2-UL19-5949 - CGGCCGGCGGCGUGUACGC 19 10332

HSV2-UL19-677 - GCGGCCGGCGGCGUGUACGC 20 917

HSV2-UL19-5950 - CGCGGCCGGCGGCGUGUACGC 21 10333

HSV2-UL19-5951 - GCGCGGCCGGCGGCGUGUACGC 22 10334

HSV2-UL19-5952 - CGCGCGGCCGGCGGCGUGUACGC 23 10335

HSV2-UL19-5953 - ACGCGCGGCCGGCGGCGUGUACGC 24 10336

HSV2-UL19-5954 - GCCGGCCCUGCAACCCGC 18 10337

HSV2-UL19-5955 - CGCCGGCCCUGCAACCCGC 19 10338

HSV2-UL19-671 - UCGCCGGCCCUGCAACCCGC 20 911

HSV2-UL19-5956 - UUCGCCGGCCCUGCAACCCGC 21 10339

HSV2-UL19-5957 - UUUCGCCGGCCCUGCAACCCGC 22 10340

HSV2-UL19-5958 - CUUUCGCCGGCCCUGCAACCCGC 23 10341

HSV2-UL19-5959 - ACUUUCGCCGGCCCUGCAACCCGC 24 10342

HSV2-UL19-5960 - GUGGUGCAGCACGCCCGC 18 10343

HSV2-UL19-5961 - CGUGGUGCAGCACGCCCGC 19 10344

HSV2-UL19-2162 - CCGUGGUGCAGCACGCCCGC 20 2456

HSV2-UL19-5962 - CCCGUGGUGCAGCACGCCCGC 21 10345

HSV2-UL19-5963 - GCCCGUGGUGCAGCACGCCCGC 22 10346

HSV2-UL19-5964 - AGCCCGUGGUGCAGCACGCCCGC 23 10347

HSV2-UL19-5965 - CAGCCCGUGGUGCAGCACGCCCGC 24 10348

HSV2-UL19-5966 - UCACGCUCACCCAGCCGC 18 10349

HSV2-UL19-5967 - U U CACG CU CACCCAG CCGC 19 10350

HSV2-UL19-2189 - CUUCACGCUCACCCAGCCGC 20 2473

HSV2-UL19-5968 - ACU U CACG CU CACCCAG CCG C 21 10351 HSV2-UL19-5969 - AACU UCACGCUCACCCAGCCGC 22 10352

HSV2-UL19-5970 - CAACUUCACGCUCACCCAGCCGC 23 10353

HSV2-UL19-5971 - UCAACUUCACGCUCACCCAGCCGC 24 10354

HSV2-UL19-5972 - AUGGGGAGGCCCACGCGC 18 10355

HSV2-UL19-5973 - GAUGGGGAGGCCCACGCGC 19 10356

HSV2-UL19-2249 - CGAUGGGGAGGCCCACGCGC 20 2506

HSV2-UL19-5974 - GCG AUGGGGAGGCCCACGCGC 21 10357

HSV2-UL19-5975 - CGCGAUGGGGAGGCCCACGCGC 22 10358

HSV2-UL19-5976 - CCGCG AUGGGGAGGCCCACGCGC 23 10359

HSV2-UL19-5977 - CCCGCGAUGGGGAGGCCCACGCGC 24 10360

HSV2-UL19-5978 - UGGUGCCGGCCU UCUCGC 18 10361

HSV2-UL19-5979 - CUGGUGCCGGCCUUCUCGC 19 10362

HSV2-UL19-554 - GCUGGUGCCGGCCUUCUCGC 20 794

HSV2-UL19-5980 - UGCUGGUGCCGGCCUUCUCGC 21 10363

HSV2-UL19-5981 - GUGCUGGUGCCGGCCUUCUCGC 22 10364

HSV2-UL19-5982 - CGUGCUGGUGCCGGCCU UCUCGC 23 10365

HSV2-UL19-5983 - ACGUGCUGGUGCCGGCCUUCUCGC 24 10366

HSV2-UL19-5984 - CUCAUGUACGACCACGGC 18 10367

HSV2-UL19-5985 - CCUCAUGUACGACCACGGC 19 10368

HSV2-UL19-2222 - CCCUCAUGUACGACCACGGC 20 2492

HSV2-UL19-5986 - GCCCUCAUGUACGACCACGGC 21 10369

HSV2-UL19-5987 - AGCCCUCAUGUACGACCACGGC 22 10370

HSV2-UL19-5988 - UAGCCCUCAUGUACGACCACGGC 23 10371

HSV2-UL19-5989 - AUAGCCCUCAUGUACGACCACGGC 24 10372

HSV2-UL19-5990 - UCGUGGCGGGAAACCGGC 18 10373

HSV2-UL19-5991 - GUCGUGGCGGGAAACCGGC 19 10374

HSV2-UL19-654 - GGUCGUGGCGGGAAACCGGC 20 894

HSV2-UL19-5992 - CGGUCGUGGCGGGAAACCGGC 21 10375

HSV2-UL19-5993 - GCGG UCGUGGCGGGAAACCGGC 22 10376

HSV2-UL19-5994 - CGCGG UCGUGGCGGGAAACCGGC 23 10377

HSV2-UL19-5995 - ACGCGGUCGUGGCGGGAAACCGGC 24 10378

HSV2-UL19-5996 - GCCCCGGCCGGCCCCGGC 18 10379

HSV2-UL19-5997 - GGCCCCGGCCGGCCCCGGC 19 10380

HSV2-UL19-2080 - CGGCCCCGGCCGGCCCCGGC 20 2401

HSV2-UL19-5998 - GCGGCCCCGGCCGGCCCCGGC 21 10381

HSV2-UL19-5999 - CGCGGCCCCGGCCGGCCCCGGC 22 10382

HSV2-UL19-6000 - UCGCGGCCCCGGCCGGCCCCGGC 23 10383

HSV2-UL19-6001 - GUCGCGGCCCCGGCCGGCCCCGGC 24 10384

HSV2-UL19-6002 - GUGCCGCGGCGCGCCGGC 18 10385

HSV2-UL19-6003 - GGUGCCGCGGCGCGCCGGC 19 10386

HSV2-UL19-2204 - AGGUGCCGCGGCGCGCCGGC 20 2483

HSV2-UL19-6004 - CAGGUGCCGCGGCGCGCCGGC 21 10387

HSV2-UL19-6005 - CCAGGUGCCGCGGCGCGCCGGC 22 10388

HSV2-UL19-6006 - CCCAGGUGCCGCGGCGCGCCGGC 23 10389

HSV2-UL19-6007 - GCCCAGGUGCCGCGGCGCGCCGGC 24 10390

HSV2-UL19-6008 - CGCCCGCGAGAGCGCGGC 18 10391 HSV2-UL19-6009 - ACGCCCGCGAGAGCGCGGC 19 10392

HSV2-UL19-602 - CACGCCCGCGAGAGCGCGGC 20 842

HSV2-UL19-6010 - GCACGCCCGCGAGAGCGCGGC 21 10393

HSV2-UL19-6011 - AGCACGCCCGCGAGAGCGCGGC 22 10394

HSV2-UL19-6012 - CAGCACGCCCGCGAGAGCGCGGC 23 10395

HSV2-UL19-6013 - GCAGCACGCCCGCGAGAGCGCGGC 24 10396

HSV2-UL19-6014 - GCCCGCCACGAAACGGGC 18 10397

HSV2-UL19-6015 - GGCCCGCCACGAAACGGGC 19 10398

HSV2-UL19-627 - UGGCCCGCCACGAAACGGGC 20 867

HSV2-UL19-6016 - GUGGCCCGCCACGAAACGGGC 21 10399

HSV2-UL19-6017 - GGUGGCCCGCCACGAAACGGGC 22 10400

HSV2-UL19-6018 - AGG UGGCCCGCCACGAAACGGGC 23 10401

HSV2-UL19-6019 - CAGGUGGCCCGCCACGAAACGGGC 24 10402

HSV2-UL19-6020 - GACUUUACCCUGCCGGGC 18 10403

HSV2-UL19-6021 - CGACUU UACCCUGCCGGGC 19 10404

HSV2-UL19-2118 - ACGACUUUACCCUGCCGGGC 20 2427

HSV2-UL19-6022 - GACGACUUUACCCUGCCGGGC 21 10405

HSV2-UL19-6023 - GGACGACUUUACCCUGCCGGGC 22 10406

HSV2-UL19-6024 - UGGACGACUUUACCCUGCCGGGC 23 10407

HSV2-UL19-6025 - GUGGACGACUUUACCCUGCCGGGC 24 10408

HSV2-UL19-6026 - UU UCGUGACGCCCGGGGC 18 10409

HSV2-UL19-6027 - GUUUCGUGACGCCCGGGGC 19 10410

HSV2-UL19-2097 - CGUUUCGUGACGCCCGGGGC 20 2413

HSV2-UL19-6028 - GCGUU UCGUGACGCCCGGGGC 21 10411

HSV2-UL19-6029 - GGCGUUUCGUGACGCCCGGGGC 22 10412

HSV2-UL19-6030 - UGGCGU UUCGUGACGCCCGGGGC 23 10413

HSV2-UL19-6031 - GUGGCGUUUCGUGACGCCCGGGGC 24 10414

HSV2-UL19-6032 - GACGCGGCCGUCGGGGGC 18 10415

HSV2-UL19-6033 - CGACGCGGCCGUCGGGGGC 19 10416

HSV2-UL19-2075 - UCGACGCGGCCGUCGGGGGC 20 2397

HSV2-UL19-6034 - AUCGACGCGGCCGUCGGGGGC 21 10417

HSV2-UL19-6035 - CAUCGACGCGGCCGUCGGGGGC 22 10418

HSV2-UL19-6036 - ACAU CGACGCGGCCGUCGGGGGC 23 10419

HSV2-UL19-6037 - AACAUCGACGCGGCCGUCGGGGGC 24 10420

HSV2-UL19-6038 - GGAU UGACGCGGGGGGGC 18 10421

HSV2-UL19-6039 - CGGAUUGACGCGGGGGGGC 19 10422

HSV2-UL19-2134 - CCGGAUUGACGCGGGGGGGC 20 2437

HSV2-UL19-6040 - GCCGGAUUGACGCGGGGGGGC 21 10423

HSV2-UL19-6041 - UGCCGGAU UGACGCGGGGGGGC 22 10424

HSV2-UL19-6042 - CUGCCGGAUUGACGCGGGGGGGC 23 10425

HSV2-UL19-6043 - ACUGCCGGAU UGACGCGGGGGGGC 24 10426

HSV2-UL19-6044 - GCGCAACGCGGUCGUGGC 18 10427

HSV2-UL19-6045 - UGCGCAACGCGGUCGUGGC 19 10428

HSV2-UL19-652 - CUGCGCAACGCGGUCGUGGC 20 892

HSV2-UL19-6046 - CCUGCGCAACGCGGUCGUGGC 21 10429

HSV2-UL19-6047 - ACCUGCGCAACGCGGUCGUGGC 22 10430 HSV2-UL19-6048 - UACCUGCGCAACGCGGUCGUGGC 23 10431

HSV2-UL19-6049 - GUACCUGCGCAACGCGGUCGUGGC 24 10432

HSV2-UL19-6050 - GCCACCUGCUGGAUAUGC 18 10433

HSV2-UL19-6051 - CGCCACCUGCUGGAUAUGC 19 10434

HSV2-UL19-399 - CCGCCACCUGCUGGAUAUGC 20 639

HSV2-UL19-6052 - GCCGCCACCUGCUGGAUAUGC 21 10435

HSV2-UL19-6053 - GGCCGCCACCUGCUGGAUAUGC 22 10436

HSV2-UL19-6054 - GGGCCGCCACCUGCUGGAUAUGC 23 10437

HSV2-UL19-6055 - UGGGCCGCCACCUGCUGGAUAUGC 24 10438

HSV2-UL19-6056 - ACGGCCAAACAUCGCUGC 18 10439

HSV2-UL19-6057 - CACGGCCAAACAUCGCUGC 19 10440

HSV2-UL19-2231 - UCACGGCCAAACAUCGCUGC 20 2497

HSV2-UL19-6058 - AUCACGGCCAAACAUCGCUGC 21 10441

HSV2-UL19-6059 - CAUCACGGCCAAACAUCGCUGC 22 10442

HSV2-UL19-6060 - ACAUCACGGCCAAACAUCGCUGC 23 10443

HSV2-UL19-6061 - GACAUCACGGCCAAACAUCGCUGC 24 10444

HSV2-UL19-6062 - GCCCCCGGUGAGGAGUGC 18 10445

HSV2-UL19-6063 - CGCCCCCGGUGAGGAGUGC 19 10446

HSV2-UL19-2148 - UCGCCCCCGGUGAGGAGUGC 20 2445

HSV2-UL19-6064 - AUCGCCCCCGGUGAGGAGUGC 21 10447

HSV2-UL19-6065 - GAUCGCCCCCGGUGAGGAGUGC 22 10448

HSV2-UL19-6066 - AG AUCGCCCCCGGUGAGGAGUGC 23 10449

HSV2-UL19-6067 - GAGAUCGCCCCCGGUGAGGAGUGC 24 10450

HSV2-UL19-6068 - CCUGGCGACCAGGGU UGC 18 10451

HSV2-UL19-6069 - GCCUGGCGACCAGGGUUGC 19 10452

HSV2-UL19-2003 - CGCCUGGCGACCAGGGU UGC 20 2348

HSV2-UL19-6070 - GCGCCUGGCGACCAGGGUUGC 21 10453

HSV2-UL19-6071 - GGCGCCUGGCGACCAGGGUUGC 22 10454

HSV2-UL19-6072 - GGGCGCCUGGCGACCAGGGUUGC 23 10455

HSV2-UL19-6073 - CGGGCGCCUGGCGACCAGGGUUGC 24 10456

HSV2-UL19-6074 - UGCCUGGAGCGUCUCAUC 18 10457

HSV2-UL19-6075 - CUGCCUGGAGCGUCUCAUC 19 10458

HSV2-UL19-2233 - GCUGCCUGGAGCGUCUCAUC 20 2498

HSV2-UL19-6076 - CGCUGCCUGGAGCGUCUCAUC 21 10459

HSV2-UL19-6077 - UCGCUGCCUGGAGCGUCUCAUC 22 10460

HSV2-UL19-6078 - AUCGCUGCCUGGAGCGUCUCAUC 23 10461

HSV2-UL19-6079 - CAUCGCUGCCUGGAGCGUCUCAUC 24 10462

HSV2-UL19-6080 - UACAUCGUGACCUACCUC 18 10463

HSV2-UL19-6081 - GUACAUCGUGACCUACCUC 19 10464

HSV2-UL19-504 - CGUACAUCGUGACCUACCUC 20 744

HSV2-UL19-6082 - UCGUACAUCGUGACCUACCUC 21 10465

HSV2-UL19-6083 - CUCGUACAUCGUGACCUACCUC 22 10466

HSV2-UL19-6084 - UCUCGUACAUCGUGACCUACCUC 23 10467

HSV2-UL19-6085 - GUCUCGUACAUCGUGACCUACCUC 24 10468

HSV2-UL19-6086 - CCGGGGUGCCGCGAGCUC 18 10469

HSV2-UL19-6087 - GCCGGGGUGCCGCGAGCUC 19 10470 HSV2-UL19-2240 - CGCCGGGGUGCCGCGAGCUC 20 2502

HSV2-UL19-6088 - CCGCCGGGGUGCCGCGAGCUC 21 10471

HSV2-UL19-6089 - CCCGCCGGGGUGCCGCGAGCUC 22 10472

HSV2-UL19-6090 - GCCCGCCGGGGUGCCGCGAGCUC 23 10473

HSV2-UL19-6091 - CGCCCGCCGGGGUGCCGCGAGCUC 24 10474

HSV2-UL19-6092 - GUGUGUCAUCCUUCGCUC 18 10475

HSV2-UL19-6093 - CGUGUGUCAUCCUUCGCUC 19 10476

HSV2-UL19-2071 - CCGUGUGUCAUCCUUCGCUC 20 2395

HSV2-UL19-6094 - ACCGUGUGUCAUCCUUCGCUC 21 10477

HSV2-UL19-6095 - GACCGUGUGUCAUCCUUCGCUC 22 10478

HSV2-UL19-6096 - GGACCGUGUGUCAUCCUUCGCUC 23 10479

HSV2-UL19-6097 - GGGACCGUGUGUCAUCCUUCGCUC 24 10480

HSV2-UL19-6098 - ACAACGCCAACCUGGCUC 18 10481

HSV2-UL19-6099 - GACAACGCCAACCUGGCUC 19 10482

HSV2-UL19-464 - CGACAACGCCAACCUGGCUC 20 704

HSV2-UL19-6100 - CCGACAACGCCAACCUGGCUC 21 10483

HSV2-UL19-6101 - CCCGACAACGCCAACCUGGCUC 22 10484

HSV2-UL19-6102 - GCCCGACAACGCCAACCUGGCUC 23 10485

HSV2-UL19-6103 - AGCCCGACAACGCCAACCUGGCUC 24 10486

HSV2-UL19-6104 - GCCAUCGAGGCCUGGCUC 18 10487

HSV2-UL19-6105 - GGCCAUCGAGGCCUGGCUC 19 10488

HSV2-UL19-2013 - AGGCCAUCGAGGCCUGGCUC 20 2357

HSV2-UL19-6106 - GAGGCCAUCGAGGCCUGGCUC 21 10489

HSV2-UL19-6107 - CGAGGCCAUCGAGGCCUGGCUC 22 10490

HSV2-UL19-6108 - GCGAGGCCAUCGAGGCCUGGCUC 23 10491

HSV2-UL19-6109 - CGCGAGGCCAUCGAGGCCUGGCUC 24 10492

HSV2-UL19-6110 - GCUGGUGCCGGCCUUCUC 18 10493

HSV2-UL19-6111 - UGCUGGUGCCGGCCUUCUC 19 10494

HSV2-UL19-2136 - GUGCUGGUGCCGGCCUUCUC 20 2439

HSV2-UL19-6112 - CGUGCUGGUGCCGGCCU UCUC 21 10495

HSV2-UL19-6113 - ACGUGCUGGUGCCGGCCUUCUC 22 10496

HSV2-UL19-6114 - UACGUGCUGGUGCCGGCCUUCUC 23 10497

HSV2-UL19-6115 - UUACGUGCUGGUGCCGGCCUUCUC 24 10498

HSV2-UL19-3512 - CGCAACGUCCAGGCCGUC 18 7895

HSV2-UL19-3513 - GCGCAACGUCCAGGCCGUC 19 7896

HSV2-UL19-3514 - CGCGCAACGUCCAGGCCGUC 20 7897

HSV2-UL19-3515 - GCGCGCAACGUCCAGGCCGUC 21 7898

HSV2-UL19-3516 - CGCGCGCAACGUCCAGGCCGUC 22 7899

HSV2-UL19-3517 - UCGCGCGCAACGUCCAGGCCGUC 23 7900

HSV2-UL19-3518 - CUCGCGCGCAACGUCCAGGCCGUC 24 7901

HSV2-UL19-6116 - AACAUCGACGCGGCCGUC 18 10499

HSV2-UL19-6117 - GAACAUCGACGCGGCCGUC 19 10500

HSV2-UL19-442 - UGAACAUCGACGCGGCCGUC 20 682

HSV2-UL19-6118 - AUG AACAUCGACGCGGCCGUC 21 10501

HSV2-UL19-6119 - CAUGAACAUCGACGCGGCCGUC 22 10502

HSV2-UL19-6120 - UCAUGAACAUCGACGCGGCCGUC 23 10503 HSV2-UL19-6121 - CUCAUGAACAUCGACGCGGCCGUC 24 10504

HSV2-UL19-6122 - AAGUCCCCCCGGCCGGUC 18 10505

HSV2-UL19-6123 - GAAGUCCCCCCGGCCGGUC 19 10506

HSV2-UL19-472 - CGAAGUCCCCCCGGCCGGUC 20 712

HSV2-UL19-6124 - GCGAAGUCCCCCCGGCCGGUC 21 10507

HSV2-UL19-6125 - GGCGAAGUCCCCCCGGCCGGUC 22 10508

HSV2-UL19-6126 - CGGCGAAGUCCCCCCGGCCGGUC 23 10509

HSV2-UL19-6127 - GCGGCGAAGUCCCCCCGGCCGGUC 24 10510

HSV2-UL19-6128 - ACCUACGGCGAGAUGGUC 18 10511

HSV2-UL19-6129 - GACCUACGGCGAGAUGGUC 19 10512

HSV2-UL19-2023 - UG ACCUACGGCGAGAUGGUC 20 2365

HSV2-UL19-6130 - GUGACCUACGGCGAGAUGGUC 21 10513

HSV2-UL19-6131 - GGUG ACCUACGGCGAGAUGGUC 22 10514

HSV2-UL19-6132 - CGGUGACCUACGGCGAGAUGGUC 23 10515

HSV2-UL19-6133 - CCGGUGACCUACGGCGAGAUGGUC 24 10516

HSV2-UL19-6134 - CUGCAGAACAUGGUGGUC 18 10517

HSV2-UL19-6135 - GCUGCAGAACAUGGUGGUC 19 10518

HSV2-UL19-2143 - CGCUGCAGAACAUGGUGGUC 20 2442

HSV2-UL19-6136 - ACGCUGCAGAACAUGGUGGUC 21 10519

HSV2-UL19-6137 - CACGCUGCAGAACAUGGUGGUC 22 10520

HSV2-UL19-6138 - CCACGCUGCAGAACAUGGUGGUC 23 10521

HSV2-UL19-6139 - GCCACGCUGCAGAACAUGGUGGUC 24 10522

HSV2-UL19-6140 - CGCCUCCUGCAGUCCUUC 18 10523

HSV2-UL19-6141 - GCGCCUCCUGCAGUCCUUC 19 10524

HSV2-UL19-2017 - AGCGCCUCCUGCAGUCCUUC 20 2360

HSV2-UL19-6142 - CAGCGCCUCCUGCAGUCCUUC 21 10525

HSV2-UL19-6143 - ACAGCGCCUCCUGCAGUCCU UC 22 10526

HSV2-UL19-6144 - AACAGCGCCUCCUGCAGUCCUUC 23 10527

HSV2-UL19-6145 - AAACAGCGCCUCCUGCAGUCCUUC 24 10528

HSV2-UL19-6146 - GGCGACAGGCUGGUCUUC 18 10529

HSV2-UL19-6147 - AGGCGACAGGCUGGUCUUC 19 10530

HSV2-UL19-2045 - UAGGCGACAGGCUGGUCUUC 20 2381

HSV2-UL19-6148 - AUAGGCGACAGGCUGGUCUUC 21 10531

HSV2-UL19-6149 - CAUAGGCGACAGGCUGGUCUUC 22 10532

HSV2-UL19-6150 - CCAUAGGCGACAGGCUGGUCUUC 23 10533

HSV2-UL19-6151 - GCCAUAGGCGACAGGCUGGUCUUC 24 10534

HSV2-UL19-6152 - CCCGCGUUCGACUUCU UC 18 10535

HSV2-UL19-6153 - CCCCGCGUUCGACUUCUUC 19 10536

HSV2-UL19-2089 - ACCCCGCGUUCGACUUCUUC 20 2408

HSV2-UL19-6154 - CACCCCGCGUUCGACUUCUUC 21 10537

HSV2-UL19-6155 - GCACCCCGCGUUCGACUUCUUC 22 10538

HSV2-UL19-6156 - UGCACCCCGCGUUCGACUUCUUC 23 10539

HSV2-UL19-6157 - CUGCACCCCGCGUUCGACUUCUUC 24 10540

HSV2-UL19-6158 - UGCGACACGAGCUUCUUC 18 10541

HSV2-UL19-6159 - UUGCGACACGAGCUUCUUC 19 10542

HSV2-UL19-2009 - UU UGCGACACGAGCUUCUUC 20 2353 HSV2-UL19-6160 - UUUUGCGACACGAGCUUCUUC 21 10543

HSV2-UL19-6161 - CUUU UGCGACACGAGCUUCUUC 22 10544

HSV2-UL19-6162 - GCUUUUGCGACACGAGCUUCUUC 23 10545

HSV2-UL19-6163 - AGCUUUUGCGACACGAGCUUCUUC 24 10546

HSV2-UL19-6164 - ACGCGAUGCGCGGCGUUC 18 10547

HSV2-UL19-6165 - CACGCGAUGCGCGGCGUUC 19 10548

HSV2-UL19-2106 - ACACGCGAUGCGCGGCGUUC 20 2419

HSV2-UL19-6166 - AACACGCGAUGCGCGGCGUUC 21 10549

HSV2-UL19-6167 - CAACACGCGAUGCGCGGCGUUC 22 10550

HSV2-UL19-6168 - ACAACACGCGAUGCGCGGCGUUC 23 10551

HSV2-UL19-6169 - AACAACACGCGAUGCGCGGCGUUC 24 10552

HSV2-UL19-6170 - GCCGUCCGCGGGGCGUUC 18 10553

HSV2-UL19-6171 - CGCCGUCCGCGGGGCGUUC 19 10554

HSV2-UL19-2102 - CCGCCGUCCGCGGGGCGUUC 20 2416

HSV2-UL19-6172 - GCCGCCGUCCGCGGGGCGUUC 21 10555

HSV2-UL19-6173 - AGCCGCCGUCCGCGGGGCGUUC 22 10556

HSV2-UL19-6174 - UAGCCGCCGUCCGCGGGGCGUUC 23 10557

HSV2-UL19-6175 - AUAGCCGCCGUCCGCGGGGCGUUC 24 10558

HSV2-UL19-6176 - ACCCGUGCGGCCUGUU UC 18 10559

HSV2-UL19-6177 - GACCCGUGCGGCCUGUUUC 19 10560

HSV2-UL19-716 - AGACCCGUGCGGCCUGU UUC 20 956

HSV2-UL19-6178 - AAGACCCGUGCGGCCUGUUUC 21 10561

HSV2-UL19-6179 - GAAGACCCGUGCGGCCUGUUUC 22 10562

HSV2-UL19-6180 - GGAAGACCCGUGCGGCCUGUUUC 23 10563

HSV2-UL19-6181 - UGGAAGACCCGUGCGGCCUGUUUC 24 10564

HSV2-UL19-6182 - GUGUACGCGGGGGACAAG 18 10565

HSV2-UL19-6183 - CGUGUACGCGGGGGACAAG 19 10566

HSV2-UL19-2218 - GCGUGUACGCGGGGGACAAG 20 2491

HSV2-UL19-6184 - GGCGUGUACGCGGGGGACAAG 21 10567

HSV2-UL19-6185 - CGGCGUGUACGCGGGGGACAAG 22 10568

HSV2-UL19-6186 - GCGGCGUGUACGCGGGGGACAAG 23 10569

HSV2-UL19-6187 - GGCGGCGUGUACGCGGGGGACAAG 24 10570

HSV2-UL19-6188 - AGCUUCUUCCUGGGCAAG 18 10571

HSV2-UL19-6189 - GAGCUUCUUCCUGGGCAAG 19 10572

HSV2-UL19-2010 - CGAGCUUCUUCCUGGGCAAG 20 2354

HSV2-UL19-6190 - ACGAGCUUCUUCCUGGGCAAG 21 10573

HSV2-UL19-6191 - CACGAGCUUCUUCCUGGGCAAG 22 10574

HSV2-UL19-6192 - ACACGAGCUUCUUCCUGGGCAAG 23 10575

HSV2-UL19-6193 - GACACGAGCU UCUUCCUGGGCAAG 24 10576

HSV2-UL19-6194 - CUGUAUCACCAGCUCAAG 18 10577

HSV2-UL19-6195 - CCUGUAUCACCAGCUCAAG 19 10578

HSV2-UL19-2172 - CCCUGUAUCACCAGCUCAAG 20 2461

HSV2-UL19-6196 - GCCCUGUAUCACCAGCUCAAG 21 10579

HSV2-UL19-6197 - GGCCCUGUAUCACCAGCUCAAG 22 10580

HSV2-UL19-6198 - UGGCCCUGUAUCACCAGCUCAAG 23 10581

HSV2-UL19-6199 - GUGGCCCUGUAUCACCAGCUCAAG 24 10582 HSV2-UL19-6200 - AUGGCGGGGUACUUCAAG 18 10583

HSV2-UL19-6201 - CAUGGCGGGGUACUUCAAG 19 10584

HSV2-UL19-2171 - UCAUGGCGGGGUACUUCAAG 20 2460

HSV2-UL19-6202 - CUCAUGGCGGGGUACUUCAAG 21 10585

HSV2-UL19-6203 - GCUCAUGGCGGGGUACUUCAAG 22 10586

HSV2-UL19-6204 - CGCUCAUGGCGGGGUACUUCAAG 23 10587

HSV2-UL19-6205 - GCGCUCAUGGCGGGGUACUUCAAG 24 10588

HSV2-UL19-6206 - CUGCAGUCCUUCCUGAAG 18 10589

HSV2-UL19-6207 - CCUGCAGUCCUUCCUGAAG 19 10590

HSV2-UL19-2018 - UCCUGCAGUCCUUCCUGAAG 20 2361

HSV2-UL19-6208 - CUCCUGCAGUCCUUCCUGAAG 21 10591

HSV2-UL19-6209 - CCUCCUGCAGUCCUUCCUGAAG 22 10592

HSV2-UL19-6210 - GCCUCCUGCAGUCCUUCCUGAAG 23 10593

HSV2-UL19-6211 - CGCCUCCUGCAGUCCUUCCUGAAG 24 10594

HSV2-UL19-6212 - CUGGUCGCCGAGCUGAAG 18 10595

HSV2-UL19-6213 - CCUGGUCGCCGAGCUGAAG 19 10596

HSV2-UL19-359 - CCCUGGUCGCCGAGCUGAAG 20 599

HSV2-UL19-6214 - ACCCUGGUCGCCGAGCUGAAG 21 10597

HSV2-UL19-6215 - GACCCUGGUCGCCGAGCUGAAG 22 10598

HSV2-UL19-6216 - CG ACCCUGGUCGCCGAGCUGAAG 23 10599

HSV2-UL19-6217 - GCGACCCUGGUCGCCGAGCUGAAG 24 10600

HSV2-UL19-6218 - CACCUGCUGGAUAUGCAG 18 10601

HSV2-UL19-6219 - CCACCUGCUGGAUAUGCAG 19 10602

HSV2-UL19-2034 - GCCACCUGCUGGAUAUGCAG 20 2373

HSV2-UL19-6220 - CGCCACCUGCUGGAUAUGCAG 21 10603

HSV2-UL19-6221 - CCGCCACCUGCUGGAUAUGCAG 22 10604

HSV2-UL19-6222 - GCCGCCACCUGCUGGAUAUGCAG 23 10605

HSV2-UL19-6223 - GGCCGCCACCUGCUGGAUAUGCAG 24 10606

HSV2-UL19-6224 - GCGACCCUGGUCGCCGAG 18 10607

HSV2-UL19-6225 - GGCGACCCUGGUCGCCGAG 19 10608

HSV2-UL19-2005 - GGGCGACCCUGGUCGCCGAG 20 2350

HSV2-UL19-6226 - CGGGCGACCCUGGUCGCCGAG 21 10609

HSV2-UL19-6227 - CCGGGCGACCCUGGUCGCCGAG 22 10610

HSV2-UL19-6228 - CCCGGGCGACCCUGGUCGCCGAG 23 10611

HSV2-UL19-6229 - GCCCGGGCGACCCUGGUCGCCGAG 24 10612

HSV2-UL19-6230 - CAGGAGGAGCAACUCGAG 18 10613

HSV2-UL19-6231 - GCAGGAGGAGCAACUCGAG 19 10614

HSV2-UL19-2037 - UGCAGGAGGAGCAACUCGAG 20 2375

HSV2-UL19-6232 - AUGCAGGAGGAGCAACUCGAG 21 10615

HSV2-UL19-6233 - UAUGCAGGAGGAGCAACUCGAG 22 10616

HSV2-UL19-6234 - AUAUGCAGGAGGAGCAACUCGAG 23 10617

HSV2-UL19-6235 - GAUAUGCAGGAGGAGCAACUCGAG 24 10618

HSV2-UL19-6236 - UACGCGGGGGACAAGGAG 18 10619

HSV2-UL19-6237 - GUACGCGGGGGACAAGGAG 19 10620

HSV2-UL19-683 - UGUACGCGGGGGACAAGGAG 20 923

HSV2-UL19-6238 - GUG UACGCGGGGGACAAGGAG 21 10621 HSV2-UL19-6239 - CGUGUACGCGGGGGACAAGGAG 22 10622

HSV2-UL19-6240 - GCGUGUACGCGGGGGACAAGGAG 23 10623

HSV2-UL19-6241 - GGCGUGUACGCGGGGGACAAGGAG 24 10624

HSV2-UL19-6242 - ACCCUGCCGGGCCCGGAG 18 10625

HSV2-UL19-6243 - UACCCUGCCGGGCCCGGAG 19 10626

HSV2-UL19-2120 - UUACCCUGCCGGGCCCGGAG 20 2428

HSV2-UL19-6244 - UUUACCCUGCCGGGCCCGGAG 21 10627

HSV2-UL19-6245 - CUUUACCCUGCCGGGCCCGGAG 22 10628

HSV2-UL19-6246 - ACUUUACCCUGCCGGGCCCGGAG 23 10629

HSV2-UL19-6247 - GACUUUACCCUGCCGGGCCCGGAG 24 10630

HSV2-UL19-6248 - GGGACCUGGUGGCCCACG 18 10631

HSV2-UL19-6249 - CGGGACCUGGUGGCCCACG 19 10632

HSV2-UL19-2115 - UCGGGACCUGGUGGCCCACG 20 2424

HSV2-UL19-6250 - AUCGGGACCUGGUGGCCCACG 21 10633

HSV2-UL19-6251 - UAUCGGGACCUGGUGGCCCACG 22 10634

HSV2-UL19-6252 - GU AUCGGGACCUGGUGGCCCACG 23 10635

HSV2-UL19-6253 - UGUAUCGGGACCUGGUGGCCCACG 24 10636

HSV2-UL19-6254 - AGCGCUUCGCCGCGCACG 18 10637

HSV2-UL19-6255 - GAGCGCUUCGCCGCGCACG 19 10638

HSV2-UL19-2056 - GGAGCGCUUCGCCGCGCACG 20 2388

HSV2-UL19-6256 - UGGAGCGCU UCGCCGCGCACG 21 10639

HSV2-UL19-6257 - AUGGAGCGCUUCGCCGCGCACG 22 10640

HSV2-UL19-6258 - CAUGGAGCGCUUCGCCGCGCACG 23 10641

HSV2-UL19-6259 - CCAUGGAGCGCUUCGCCGCGCACG 24 10642

HSV2-UL19-6260 - UCGCGGGCGUGGCCGACG 18 10643

HSV2-UL19-6261 - UUCGCGGGCGUGGCCGACG 19 10644

HSV2-UL19-2090 - CUUCGCGGGCGUGGCCGACG 20 2409

HSV2-UL19-6262 - UCUUCGCGGGCGUGGCCGACG 21 10645

HSV2-UL19-6263 - UUCU UCGCGGGCGUGGCCGACG 22 10646

HSV2-UL19-6264 - CUUCUUCGCGGGCGUGGCCGACG 23 10647

HSV2-UL19-6265 - ACUUCUUCGCGGGCGUGGCCGACG 24 10648

HSV2-UL19-6266 - GAGACUGCCGGAUUGACG 18 10649

HSV2-UL19-6267 - CGAGACUGCCGGAUUGACG 19 10650

HSV2-UL19-533 - CCGAGACUGCCGGAUUGACG 20 773

HSV2-UL19-6268 - ACCGAGACUGCCGGAUUGACG 21 10651

HSV2-UL19-6269 - CACCGAGACUGCCGGAUUGACG 22 10652

HSV2-UL19-6270 - CCACCGAGACUGCCGGAU UGACG 23 10653

HSV2-UL19-6271 - GCCACCGAGACUGCCGGAUUGACG 24 10654

HSV2-UL19-6272 - ACGUGCCGGUGACCUACG 18 10655

HSV2-UL19-6273 - GACGUGCCGGUGACCUACG 19 10656

HSV2-UL19-2022 - CGACGUGCCGGUGACCUACG 20 2364

HSV2-UL19-6274 - CCGACGUGCCGGUGACCUACG 21 10657

HSV2-UL19-6275 - GCCGACGUGCCGGUGACCUACG 22 10658

HSV2-UL19-6276 - GGCCGACGUGCCGGUGACCUACG 23 10659

HSV2-UL19-6277 - AGGCCGACGUGCCGGUGACCUACG 24 10660

HSV2-UL19-6278 - CGGCCGGCGGCGUGUACG 18 10661 HSV2-UL19-6279 - GCGGCCGGCGGCGUGUACG 19 10662

HSV2-UL19-676 - CGCGGCCGGCGGCGUGUACG 20 916

HSV2-UL19-6280 - GCGCGGCCGGCGGCGUGUACG 21 10663

HSV2-UL19-6281 - CGCGCGGCCGGCGGCGUGUACG 22 10664

HSV2-UL19-6282 - ACGCGCGGCCGGCGGCGUGUACG 23 10665

HSV2-UL19-6283 - GACGCGCGGCCGGCGGCGUGUACG 24 10666

HSV2-UL19-6284 - GGGGGCGCUGCUGCACCG 18 10667

HSV2-UL19-6285 - CGGGGGCGCUGCUGCACCG 19 10668

HSV2-UL19-557 - GCGGGGGCGCUGCUGCACCG 20 797

HSV2-UL19-6286 - CGCGGGGGCGCUGCUGCACCG 21 10669

HSV2-UL19-6287 - UCGCGGGGGCGCUGCUGCACCG 22 10670

HSV2-UL19-6288 - CUCGCGGGGGCGCUGCUGCACCG 23 10671

HSV2-UL19-6289 - UCUCGCGGGGGCGCUGCUGCACCG 24 10672

HSV2-UL19-6290 - CCGAGUGCCAGAUGACCG 18 10673

HSV2-UL19-6291 - GCCGAGUGCCAGAUGACCG 19 10674

HSV2-UL19-462 - CGCCGAGUGCCAGAUGACCG 20 702

HSV2-UL19-6292 - UCGCCGAGUGCCAGAUGACCG 21 10675

HSV2-UL19-6293 - GUCGCCGAGUGCCAGAUGACCG 22 10676

HSV2-UL19-6294 - GGUCGCCGAGUGCCAGAUGACCG 23 10677

HSV2-UL19-6295 - GGGUCGCCGAGUGCCAGAUGACCG 24 10678

HSV2-UL19-6296 - CGCCGGCCCUGCAACCCG 18 10679

HSV2-UL19-6297 - UCGCCGGCCCUGCAACCCG 19 10680

HSV2-UL19-670 - UUCGCCGGCCCUGCAACCCG 20 910

HSV2-UL19-6298 - UUUCGCCGGCCCUGCAACCCG 21 10681

HSV2-UL19-6299 - CUUUCGCCGGCCCUGCAACCCG 22 10682

HSV2-UL19-6300 - ACUU UCGCCGGCCCUGCAACCCG 23 10683

HSV2-UL19-6301 - U ACU U UCGCCGGCCCU GCAACCCG 24 10684

HSV2-UL19-6302 - GCGUGAACCACGACCCCG 18 10685

HSV2-UL19-6303 - GGCGUGAACCACGACCCCG 19 10686

HSV2-UL19-2076 - GGGCGUGAACCACGACCCCG 20 2398

HSV2-UL19-6304 - GGGGCGUGAACCACGACCCCG 21 10687

HSV2-UL19-6305 - GGGGGCGUGAACCACGACCCCG 22 10688

HSV2-UL19-6306 - CGGGGGCGUGAACCACGACCCCG 23 10689

HSV2-UL19-6307 - UCGGGGGCGUGAACCACGACCCCG 24 10690

HSV2-UL19-6308 - UCCCGGAGAUCGCCCCCG 18 10691

HSV2-UL19-6309 - GUCCCGGAGAUCGCCCCCG 19 10692

HSV2-UL19-2145 - GGUCCCGGAGAUCGCCCCCG 20 2443

HSV2-UL19-6310 - UGGUCCCGGAGAUCGCCCCCG 21 10693

HSV2-UL19-6311 - GUGGUCCCGGAGAUCGCCCCCG 22 10694

HSV2-UL19-6312 - GGUGGUCCCGGAGAUCGCCCCCG 23 10695

HSV2-UL19-6313 - UGGUGGUCCCGGAGAUCGCCCCCG 24 10696

HSV2-UL19-6314 - GUGCAGUUUAAGCGCCCG 18 10697

HSV2-UL19-6315 - CGUGCAGUUUAAGCGCCCG 19 10698

HSV2-UL19-2237 - ACGUGCAGUUUAAGCGCCCG 20 2500

HSV2-UL19-6316 - GACGUGCAGUUUAAGCGCCCG 21 10699

HSV2-UL19-6317 - CGACGUGCAGUUUAAGCGCCCG 22 10700 HSV2-UL19-6318 - GCGACGUGCAGUUUAAGCGCCCG 23 10701

HSV2-UL19-6319 - AGCGACGUGCAGUUUAAGCGCCCG 24 10702

HSV2-UL19-6320 - UGAACAUCGACGCGGCCG 18 10703

HSV2-UL19-6321 - AUGAACAUCGACGCGGCCG 19 10704

HSV2-UL19-2072 - CAUGAACAUCGACGCGGCCG 20 2396

HSV2-UL19-6322 - UCAUGAACAUCGACGCGGCCG 21 10705

HSV2-UL19-6323 - CUCAUGAACAUCGACGCGGCCG 22 10706

HSV2-UL19-6324 - GCUCAUGAACAUCGACGCGGCCG 23 10707

HSV2-UL19-6325 - CGCUCAUGAACAUCGACGCGGCCG 24 10708

HSV2-UL19-6326 - AAACU U U UACCUCGGCCG 18 10709

HSV2-UL19-6327 - AAAACUUUUACCUCGGCCG 19 10710

HSV2-UL19-645 - CAAAACUUUUACCUCGGCCG 20 885

HSV2-UL19-6328 - CCAAAACUUUUACCUCGGCCG 21 10711

HSV2-UL19-6329 - CCCAAAACUUUUACCUCGGCCG 22 10712

HSV2-UL19-6330 - CCCCAAAACUU UUACCUCGGCCG 23 10713

HSV2-UL19-6331 - CCCCCAAAACU U U UACCUCGGCCG 24 10714

HSV2-UL19-6332 - UACCAUAGCCGCCGUCCG 18 10715

HSV2-UL19-6333 - CUACCAUAGCCGCCGUCCG 19 10716

HSV2-UL19-491 - GCUACCAUAGCCGCCGUCCG 20 731

HSV2-UL19-6334 - GGCUACCAUAGCCGCCGUCCG 21 10717

HSV2-UL19-6335 - CGGCUACCAUAGCCGCCGUCCG 22 10718

HSV2-UL19-6336 - CCGGCUACCAUAGCCGCCGUCCG 23 10719

HSV2-UL19-6337 - GCCGGCUACCAUAGCCGCCGUCCG 24 10720

HSV2-UL19-6338 - GACUGCCGGAUUGACGCG 18 10721

HSV2-UL19-6339 - AGACUGCCGGAUUGACGCG 19 10722

HSV2-UL19-535 - GAGACUGCCGGAUUGACGCG 20 775

HSV2-UL19-6340 - CG AGACUGCCGGAUUGACGCG 21 10723

HSV2-UL19-6341 - CCG AGACUGCCGGAUUGACGCG 22 10724

HSV2-UL19-6342 - ACCG AGACUGCCGGAUUGACGCG 23 10725

HSV2-UL19-6343 - CACCGAGACUGCCGGAUUGACGCG 24 10726

HSV2-UL19-6344 - GCCGGCGGCGUGUACGCG 18 10727

HSV2-UL19-6345 - GGCCGGCGGCGUGUACGCG 19 10728

HSV2-UL19-678 - CGGCCGGCGGCGUGUACGCG 20 918

HSV2-UL19-6346 - GCGGCCGGCGGCGUGUACGCG 21 10729

HSV2-UL19-6347 - CGCGGCCGGCGGCGUGUACGCG 22 10730

HSV2-UL19-6348 - GCGCGGCCGGCGGCGUGUACGCG 23 10731

HSV2-UL19-6349 - CGCGCGGCCGGCGGCGUGUACGCG 24 10732

HSV2-UL19-6350 - UGCUACGCAGCGCCCGCG 18 10733

HSV2-UL19-6351 - CUGCUACGCAGCGCCCGCG 19 10734

HSV2-UL19-2244 - CCUGCUACGCAGCGCCCGCG 20 2504

HSV2-UL19-6352 - CCCUGCUACGCAGCGCCCGCG 21 10735

HSV2-UL19-6353 - GCCCUGCUACGCAGCGCCCGCG 22 10736

HSV2-UL19-6354 - CGCCCUGCUACGCAGCGCCCGCG 23 10737

HSV2-UL19-6355 - CCGCCCUGCUACGCAGCGCCCGCG 24 10738

HSV2-UL19-6356 - CACG CU CACCCAG CCG CG 18 10739

HSV2-UL19-6357 - UCACGCUCACCCAGCCGCG 19 10740 HSV2-UL19-632 - UUCACGCUCACCCAGCCGCG 20 872

HSV2-UL19-6358 - CUUCACGCUCACCCAGCCGCG 21 10741

HSV2-UL19-6359 - ACUUCACGCUCACCCAGCCGCG 22 10742

HSV2-UL19-6360 - AACUUCACGCUCACCCAGCCGCG 23 10743

HSV2-UL19-6361 - CAACUUCACGCUCACCCAGCCGCG 24 10744

HSV2-UL19-6362 - CACGCCCGCGAGAGCGCG 18 10745

HSV2-UL19-6363 - GCACGCCCGCGAGAGCGCG 19 10746

HSV2-UL19-2163 - AGCACGCCCGCGAGAGCGCG 20 2457

HSV2-UL19-6364 - CAGCACGCCCGCGAGAGCGCG 21 10747

HSV2-UL19-6365 - GCAGCACGCCCGCGAGAGCGCG 22 10748

HSV2-UL19-6366 - UGCAGCACGCCCGCGAGAGCGCG 23 10749

HSV2-UL19-6367 - G U GCAG CACG CCCG CG AG AG CGCG 24 10750

HSV2-UL19-6368 - CUGUUUUCCGAGCGCGCG 18 10751

HSV2-UL19-6369 - GCUGU UUUCCGAGCGCGCG 19 10752

HSV2-UL19-2179 - UGCUGUUUUCCGAGCGCGCG 20 2468

HSV2-UL19-6370 - GUGCUGUUUUCCGAGCGCGCG 21 10753

HSV2-UL19-6371 - CGUGCUGU UUUCCGAGCGCGCG 22 10754

HSV2-UL19-6372 - ACGUGCUGUUUUCCGAGCGCGCG 23 10755

HSV2-UL19-6373 - AACGUGCUGUUUUCCGAGCGCGCG 24 10756

HSV2-UL19-6374 - ACGUCGAGCUUCCCGGCG 18 10757

HSV2-UL19-6375 - GACGUCGAGCUUCCCGGCG 19 10758

HSV2-UL19-2091 - CGACGUCGAGCUUCCCGGCG 20 2410

HSV2-UL19-6376 - CCGACGUCGAGCUUCCCGGCG 21 10759

HSV2-UL19-6377 - GCCGACGUCGAGCUUCCCGGCG 22 10760

HSV2-UL19-6378 - GGCCGACGUCGAGCUUCCCGGCG 23 10761

HSV2-UL19-6379 - UGGCCGACGUCGAGCUUCCCGGCG 24 10762

HSV2-UL19-6380 - GCCCGCGAGAGCGCGGCG 18 10763

HSV2-UL19-6381 - CGCCCGCGAGAGCGCGGCG 19 10764

HSV2-UL19-603 - ACGCCCGCGAGAGCGCGGCG 20 843

HSV2-UL19-6382 - CACGCCCGCGAGAGCGCGGCG 21 10765

HSV2-UL19-6383 - GCACGCCCGCGAGAGCGCGGCG 22 10766

HSV2-UL19-6384 - AGCACGCCCGCGAGAGCGCGGCG 23 10767

HSV2-UL19-6385 - CAGCACGCCCGCGAGAGCGCGGCG 24 10768

HSV2-UL19-6386 - CCCG CCACG AAACGG GCG 18 10769

HSV2-UL19-6387 - GCCCGCCACGAAACGGGCG 19 10770

HSV2-UL19-628 - GGCCCGCCACGAAACGGGCG 20 868

HSV2-UL19-6388 - UGGCCCGCCACGAAACGGGCG 21 10771

HSV2-UL19-6389 - GUGGCCCGCCACGAAACGGGCG 22 10772

HSV2-UL19-6390 - GGUGGCCCGCCACGAAACGGGCG 23 10773

HSV2-UL19-6391 - AGGUGGCCCGCCACGAAACGGGCG 24 10774

HSV2-UL19-6392 - GCCUGGAGCGUCUCAUCG 18 10775

HSV2-UL19-6393 - UGCCUGGAGCGUCUCAUCG 19 10776

HSV2-UL19-705 - CUGCCUGGAGCGUCUCAUCG 20 945

HSV2-UL19-6394 - GCUGCCUGGAGCGUCUCAUCG 21 10777

HSV2-UL19-6395 - CGCUGCCUGGAGCGUCUCAUCG 22 10778

HSV2-UL19-6396 - UCGCUGCCUGGAGCGUCUCAUCG 23 10779 HSV2-UL19-6397 - AUCGCUGCCUGGAGCGUCUCAUCG 24 10780

HSV2-UL19-6398 - CGGGGUGCCGCGAGCUCG 18 10781

HSV2-UL19-6399 - CCGGGGUGCCGCGAGCUCG 19 10782

HSV2-UL19-714 - GCCGGGGUGCCGCGAGCUCG 20 954

HSV2-UL19-6400 - CGCCGGGGUGCCGCGAGCUCG 21 10783

HSV2-UL19-6401 - CCGCCGGGGUGCCGCGAGCUCG 22 10784

HSV2-UL19-6402 - CCCGCCGGGGUGCCGCGAGCUCG 23 10785

HSV2-UL19-6403 - GCCCGCCGGGGUGCCGCGAGCUCG 24 10786

HSV2-UL19-6404 - CUGGUGCCGGCCUUCUCG 18 10787

HSV2-UL19-6405 - GCUGGUGCCGGCCUUCUCG 19 10788

HSV2-UL19-553 - UGCUGGUGCCGGCCUUCUCG 20 793

HSV2-UL19-6406 - GUGCUGGUGCCGGCCUUCUCG 21 10789

HSV2-UL19-6407 - CGUGCUGGUGCCGGCCUUCUCG 22 10790

HSV2-UL19-6408 - ACGUGCUGGUGCCGGCCUUCUCG 23 10791

HSV2-UL19-6409 - UACGUGCUGGUGCCGGCCUUCUCG 24 10792

HSV2-UL19-6410 - ACGGGCGCGUCGUCGUCG 18 10793

HSV2-UL19-6411 - AACGGGCGCGUCGUCGUCG 19 10794

HSV2-UL19-2150 - CAACGGGCGCGUCGUCGUCG 20 2447

HSV2-UL19-6412 - ACAACGGGCGCGUCGUCGUCG 21 10795

HSV2-UL19-6413 - CACAACGGGCGCGUCGUCGUCG 22 10796

HSV2-UL19-6414 - CCACAACGGGCGCGUCGUCGUCG 23 10797

HSV2-UL19-6415 - UCCACAACGGGCGCGUCGUCGUCG 24 10798

HSV2-UL19-6416 - CGCGCGGGCGGCCGGUCG 18 10799

HSV2-UL19-6417 - ACGCGCGGGCGGCCGGUCG 19 10800

HSV2-UL19-2015 - CACGCGCGGGCGGCCGGUCG 20 2359

HSV2-UL19-6418 - ACACGCGCGGGCGGCCGGUCG 21 10801

HSV2-UL19-6419 - GACACGCGCGGGCGGCCGGUCG 22 10802

HSV2-UL19-6420 - CGACACGCGCGGGCGGCCGGUCG 23 10803

HSV2-UL19-6421 - CCGACACGCGCGGGCGGCCGGUCG 24 10804

HSV2-UL19-6422 - CCCGGGCGACCCUGGUCG 18 10805

HSV2-UL19-6423 - GCCCGGGCGACCCUGGUCG 19 10806

HSV2-UL19-2004 - UGCCCGGGCGACCCUGGUCG 20 2349

HSV2-UL19-6424 - UUGCCCGGGCGACCCUGGUCG 21 10807

HSV2-UL19-6425 - GUUGCCCGGGCGACCCUGGUCG 22 10808

HSV2-UL19-6426 - GGUUGCCCGGGCGACCCUGGUCG 23 10809

HSV2-UL19-6427 - GGGUUGCCCGGGCGACCCUGGUCG 24 10810

HSV2-UL19-6428 - CCAACAU G CG CAU CU U CG 18 10811

HSV2-UL19-6429 - GCCAACAU G CG CAU CU U CG 19 10812

HSV2-UL19-2153 - GGCCAACAUGCGCAUCUUCG 20 2450

HSV2-UL19-6430 - CGGCCAACAUGCGCAUCUUCG 21 10813

HSV2-UL19-6431 - ACGG CCAACAU G CGCAUCUUCG 22 10814

HSV2-UL19-6432 - GACGGCCAACAUGCGCAUCUUCG 23 10815

HSV2-UL19-6433 - CGACGGCCAACAUGCGCAUCUUCG 24 10816

HSV2-UL19-6434 - UGUACGCGGGGGACAAGG 18 10817

HSV2-UL19-6435 - GUGUACGCGGGGGACAAGG 19 10818

HSV2-UL19-681 - CGUGUACGCGGGGGACAAGG 20 921 HSV2-UL19-6436 - GCGUGUACGCGGGGGACAAGG 21 10819

HSV2-UL19-6437 - GGCGUGUACGCGGGGGACAAGG 22 10820

HSV2-UL19-6438 - CGGCGUGUACGCGGGGGACAAGG 23 10821

HSV2-UL19-6439 - GCGGCGUGUACGCGGGGGACAAGG 24 10822

HSV2-UL19-6440 - UGCAGUCCUUCCUGAAGG 18 10823

HSV2-UL19-6441 - CUGCAGUCCU UCCUGAAGG 19 10824

HSV2-UL19-380 - CCUGCAGUCCUUCCUGAAGG 20 620

HSV2-UL19-6442 - UCCUGCAGUCCUUCCUGAAGG 21 10825

HSV2-UL19-6443 - CUCCUGCAGUCCUUCCUGAAGG 22 10826

HSV2-UL19-6444 - CCUCCUGCAGUCCUUCCUGAAGG 23 10827

HSV2-UL19-6445 - GCCUCCUGCAGUCCUUCCUGAAGG 24 10828

HSV2-UL19-6446 - ACCUGCUGGAUAUGCAGG 18 10829

HSV2-UL19-6447 - CACCUGCUGGAUAUGCAGG 19 10830

HSV2-UL19-400 - CCACCUGCUGGAUAUGCAGG 20 640

HSV2-UL19-6448 - GCCACCUGCUGGAUAUGCAGG 21 10831

HSV2-UL19-6449 - CGCCACCUGCUGGAUAUGCAGG 22 10832

HSV2-UL19-6450 - CCGCCACCUGCUGGAUAUGCAGG 23 10833

HSV2-UL19-6451 - GCCGCCACCUGCUGGAUAUGCAGG 24 10834

HSV2-UL19-6452 - UGGGCGGGCAGGCUCAGG 18 10835

HSV2-UL19-6453 - CUGGGCGGGCAGGCUCAGG 19 10836

HSV2-UL19-2122 - GCUGGGCGGGCAGGCUCAGG 20 2430

HSV2-UL19-6454 - AGCUGGGCGGGCAGGCUCAGG 21 10837

HSV2-UL19-6455 - GAGCUGGGCGGGCAGGCUCAGG 22 10838

HSV2-UL19-6456 - GGAGCUGGGCGGGCAGGCUCAGG 23 10839

HSV2-UL19-6457 - CGGAGCUGGGCGGGCAGGCUCAGG 24 10840

HSV2-UL19-6458 - UGGCCCGCCACGAAACGG 18 10841

HSV2-UL19-6459 - GUGGCCCGCCACGAAACGG 19 10842

HSV2-UL19-2184 - GGUGGCCCGCCACGAAACGG 20 2472

HSV2-UL19-6460 - AGG UGGCCCGCCACGAAACGG 21 10843

HSV2-UL19-6461 - CAG G U G GCCCGCCACG AAACGG 22 10844

HSV2-UL19-6462 - CCAGGUGGCCCGCCACGAAACGG 23 10845

HSV2-UL19-6463 - UCCAGGUGGCCCGCCACGAAACGG 24 10846

HSV2-UL19-6464 - GUCGUGGCGGGAAACCGG 18 10847

HSV2-UL19-6465 - GGUCGUGGCGGGAAACCGG 19 10848

HSV2-UL19-2202 - CGGUCGUGGCGGGAAACCGG 20 2482

HSV2-UL19-6466 - GCGGUCGUGGCGGGAAACCGG 21 10849

HSV2-UL19-6467 - CGCGGUCGUGGCGGGAAACCGG 22 10850

HSV2-UL19-6468 - ACGCGGUCGUGGCGGGAAACCGG 23 10851

HSV2-UL19-6469 - AACGCGGUCGUGGCGGGAAACCGG 24 10852

HSV2-UL19-6470 - UGUCCAUGGAGAACGCGG 18 10853

HSV2-UL19-6471 - CUGUCCAUGGAGAACGCGG 19 10854

HSV2-UL19-2069 - GCUGUCCAUGGAGAACGCGG 20 2394

HSV2-UL19-6472 - GGCUGUCCAUGGAGAACGCGG 21 10855

HSV2-UL19-6473 - CGGCUGUCCAUGGAGAACGCGG 22 10856

HSV2-UL19-6474 - GCGGCUGUCCAUGGAGAACGCGG 23 10857

HSV2-UL19-6475 - UGCGGCUGUCCAUGGAGAACGCGG 24 10858 HSV2-UL19-6476 - ACUGCCGGAUUGACGCGG 18 10859

HSV2-UL19-6477 - GACUGCCGGAUUGACGCGG 19 10860

HSV2-UL19-536 - AGACUGCCGGAUUGACGCGG 20 776

HSV2-UL19-6478 - GAG ACUGCCGGAUUGACGCGG 21 10861

HSV2-UL19-6479 - CGAGACUGCCGGAUUGACGCGG 22 10862

HSV2-UL19-6480 - CCGAGACUGCCGGAUUGACGCGG 23 10863

HSV2-UL19-6481 - ACCGAGACUGCCGGAUUGACGCGG 24 10864

HSV2-UL19-6482 - ACGCCCGCGAGAGCGCGG 18 10865

HSV2-UL19-6483 - CACGCCCGCGAGAGCGCGG 19 10866

HSV2-UL19-601 - GCACGCCCGCGAGAGCGCGG 20 841

HSV2-UL19-6484 - AGCACGCCCGCGAGAGCGCGG 21 10867

HSV2-UL19-6485 - CAGCACGCCCGCGAGAGCGCGG 22 10868

HSV2-UL19-6486 - GCAGCACGCCCGCGAGAGCGCGG 23 10869

HSV2-UL19-6487 - UGCAGCACGCCCGCGAGAGCGCGG 24 10870

HSV2-UL19-6488 - CCCGCGAGAGCGCGGCGG 18 10871

HSV2-UL19-6489 - GCCCGCGAGAGCGCGGCGG 19 10872

HSV2-UL19-604 - CGCCCGCGAGAGCGCGGCGG 20 844

HSV2-UL19-6490 - ACGCCCGCGAGAGCGCGGCGG 21 10873

HSV2-UL19-6491 - CACGCCCGCGAGAGCGCGGCGG 22 10874

HSV2-UL19-6492 - GCACGCCCGCGAGAGCGCGGCGG 23 10875

HSV2-UL19-6493 - AGCACGCCCGCGAGAGCGCGGCGG 24 10876

HSV2-UL19-6494 - GGGUUCACCGUCGUGCGG 18 10877

HSV2-UL19-6495 - CGGGUUCACCGUCGUGCGG 19 10878

HSV2-UL19-2175 - UCGGGUUCACCGUCGUGCGG 20 2464

HSV2-UL19-6496 - UUCGGGUUCACCGUCGUGCGG 21 10879

HSV2-UL19-6497 - GUUCGGGUUCACCGUCGUGCGG 22 10880

HSV2-UL19-6498 - GGUUCGGGUUCACCGUCGUGCGG 23 10881

HSV2-UL19-6499 - GGGUUCGGGUUCACCGUCGUGCGG 24 10882

HSV2-UL19-6500 - GGCCCGCCACGAAACGGG 18 10883

HSV2-UL19-6501 - UGGCCCGCCACGAAACGGG 19 10884

HSV2-UL19-626 - GUGGCCCGCCACGAAACGGG 20 866

HSV2-UL19-6502 - GGUGGCCCGCCACGAAACGGG 21 10885

HSV2-UL19-6503 - AGGUGGCCCGCCACGAAACGGG 22 10886

HSV2-UL19-6504 - CAGGUGGCCCGCCACGAAACGGG 23 10887

HSV2-UL19-6505 - CCAGGUGGCCCGCCACGAAACGGG 24 10888

HSV2-UL19-6506 - CGCGAGAGCGCGGCGGGG 18 10889

HSV2-UL19-6507 - CCGCGAGAGCGCGGCGGGG 19 10890

HSV2-UL19-2168 - CCCGCGAGAGCGCGGCGGGG 20 2458

HSV2-UL19-6508 - GCCCGCGAGAGCGCGGCGGGG 21 10891

HSV2-UL19-6509 - CGCCCGCGAGAGCGCGGCGGGG 22 10892

HSV2-UL19-6510 - ACGCCCGCGAGAGCGCGGCGGGG 23 10893

HSV2-UL19-6511 - CACGCCCGCGAGAGCGCGGCGGGG 24 10894

HSV2-UL19-6512 - UGCUGGCGCACAACAUGG 18 10895

HSV2-UL19-6513 - GUGCUGGCGCACAACAUGG 19 10896

HSV2-UL19-2151 - GGUGCUGGCGCACAACAUGG 20 2448

HSV2-UL19-6514 - AGGUGCUGGCGCACAACAUGG 21 10897 HSV2-UL19-6515 - CAGGUGCUGGCG CACAACAU G G 22 10898

HSV2-UL19-6516 - GCAGGUGCUGGCGCACAACAUGG 23 10899

HSV2-UL19-6517 - UGCAGGUGCUGGCGCACAACAUGG 24 10900

HSV2-UL19-6518 - UGCCGGGCCCGGAGCUGG 18 10901

HSV2-UL19-6519 - CUGCCGGGCCCGGAGCUGG 19 10902

HSV2-UL19-2121 - CCUGCCGGGCCCGGAGCUGG 20 2429

HSV2-UL19-6520 - CCCUGCCGGGCCCGGAGCUGG 21 10903

HSV2-UL19-6521 - ACCCUGCCGGGCCCGGAGCUGG 22 10904

HSV2-UL19-6522 - UACCCUGCCGGGCCCGGAGCUGG 23 10905

HSV2-UL19-6523 - UUACCCUGCCGGGCCCGGAGCUGG 24 10906

HSV2-UL19-6524 - CGAACCGGGAGACGCUGG 18 10907

HSV2-UL19-6525 - GCGAACCGGGAGACGCUGG 19 10908

HSV2-UL19-2042 - GGCGAACCGGGAGACGCUGG 20 2378

HSV2-UL19-6526 - AGGCGAACCGGGAGACGCUGG 21 10909

HSV2-UL19-6527 - GAGGCGAACCGGGAGACGCUGG 22 10910

HSV2-UL19-6528 - CGAGGCGAACCGGGAGACGCUGG 23 10911

HSV2-UL19-6529 - UCGAGGCGAACCGGGAGACGCUGG 24 10912

HSV2-UL19-6530 - UGCGCAACGCGGUCGUGG 18 10913

HSV2-UL19-6531 - CUGCGCAACGCGGUCGUGG 19 10914

HSV2-UL19-651 - CCUGCGCAACGCGGUCGUGG 20 891

HSV2-UL19-6532 - ACCUGCGCAACGCGGUCGUGG 21 10915

HSV2-UL19-6533 - UACCUGCGCAACGCGGUCGUGG 22 10916

HSV2-UL19-6534 - GUACCUGCGCAACGCGGUCGUGG 23 10917

HSV2-UL19-6535 - UGUACCUGCGCAACGCGGUCGUGG 24 10918

HSV2-UL19-6536 - GUUCUGCGGCUGUCCAUG 18 10919

HSV2-UL19-6537 - GGUUCUGCGGCUGUCCAUG 19 10920

HSV2-UL19-2068 - AGGU UCUGCGGCUGUCCAUG 20 2393

HSV2-UL19-6538 - CAGGUUCUGCGGCUGUCCAUG 21 10921

HSV2-UL19-6539 - CCAGGUUCUGCGGCUGUCCAUG 22 10922

HSV2-UL19-6540 - ACCAGGU UCUGCGGCUGUCCAUG 23 10923

HSV2-UL19-6541 - CACCAGGUUCUGCGGCUGUCCAUG 24 10924

HSV2-UL19-6542 - CUGACCUACGCGCUCAUG 18 10925

HSV2-UL19-6543 - GCUGACCUACGCGCUCAUG 19 10926

HSV2-UL19-2169 - CGCUGACCUACGCGCUCAUG 20 2459

HSV2-UL19-6544 - GCGCUGACCUACGCGCUCAUG 21 10927

HSV2-UL19-6545 - CGCGCUGACCUACGCGCUCAUG 22 10928

HSV2-UL19-6546 - ACGCGCUGACCUACGCGCUCAUG 23 10929

HSV2-UL19-6547 - AACGCGCUGACCUACGCGCUCAUG 24 10930

HSV2-UL19-6548 - UACGCAGCGCCCGCGAUG 18 10931

HSV2-UL19-6549 - CUACGCAGCGCCCGCGAUG 19 10932

HSV2-UL19-719 - GCUACGCAGCGCCCGCGAUG 20 959

HSV2-UL19-6550 - UGCUACGCAGCGCCCGCGAUG 21 10933

HSV2-UL19-6551 - CUGCUACGCAGCGCCCGCGAUG 22 10934

HSV2-UL19-6552 - CCUGCUACGCAGCGCCCGCGAUG 23 10935

HSV2-UL19-6553 - CCCUGCUACGCAGCGCCCGCGAUG 24 10936

HSV2-UL19-6554 - CGCCACCUGCUGGAUAUG 18 10937 HSV2-UL19-6555 - CCGCCACCUGCUGGAUAUG 19 10938

HSV2-UL19-2032 - GCCGCCACCUGCUGGAUAUG 20 2372

HSV2-UL19-6556 - GGCCGCCACCUGCUGGAUAUG 21 10939

HSV2-UL19-6557 - GGGCCGCCACCUGCUGGAUAUG 22 10940

HSV2-UL19-6558 - UGGGCCGCCACCUGCUGGAUAUG 23 10941

HSV2-UL19-6559 - GUGGGCCGCCACCUGCUGGAUAUG 24 10942

HSV2-UL19-6560 - GUGGACGACUUUACCCUG 18 10943

HSV2-UL19-6561 - GGUGGACGACUUUACCCUG 19 10944

HSV2-UL19-2117 - UGGUGGACGACUUUACCCUG 20 2426

HSV2-UL19-6562 - CUGGUGGACGACUUUACCCUG 21 10945

HSV2-UL19-6563 - GCUGGUGGACGACUUUACCCUG 22 10946

HSV2-UL19-6564 - AGCUGGUGGACGACUUUACCCUG 23 10947

HSV2-UL19-6565 - CAGCUGGUGGACGACUUUACCCUG 24 10948

HSV2-UL19-6566 - AACGUGCCCUACCCCCUG 18 10949

HSV2-UL19-6567 - CAACG U G CCCU ACCCCCU G 19 10950

HSV2-UL19-2050 - CCAACGUGCCCUACCCCCUG 20 2384

HSV2-UL19-6568 - ACCAACG U G CCCU ACCCCCUG 21 10951

HSV2-UL19-6569 - CACCAACG U GCCCU ACCCCCU G 22 10952

HSV2-UL19-6570 - CCACCAACG UG CCCU ACCCCCU G 23 10953

HSV2-UL19-6571 - GCCACCAACGUGCCCUACCCCCUG 24 10954

HSV2-UL19-6572 - GUCUUCCUGGAGGCCCUG 18 10955

HSV2-UL19-6573 - GGUCU UCCUGGAGGCCCUG 19 10956

HSV2-UL19-2049 - UGGUCUUCCUGGAGGCCCUG 20 2383

HSV2-UL19-6574 - CUGGUCUUCCUGGAGGCCCUG 21 10957

HSV2-UL19-6575 - GCUGGUCU UCCUGGAGGCCCUG 22 10958

HSV2-UL19-6576 - GGCUGGUCUUCCUGGAGGCCCUG 23 10959

HSV2-UL19-6577 - AGGCUGGUCUUCCUGGAGGCCCUG 24 10960

HSV2-UL19-6578 - GAGGCCCUGGCCCAGCUG 18 10961

HSV2-UL19-6579 - CGAGGCCCUGGCCCAGCUG 19 10962

HSV2-UL19-2116 - UCGAGGCCCUGGCCCAGCUG 20 2425

HSV2-UL19-6580 - GUCGAGGCCCUGGCCCAGCUG 21 10963

HSV2-UL19-6581 - CGUCGAGGCCCUGGCCCAGCUG 22 10964

HSV2-UL19-6582 - ACGUCGAGGCCCUGGCCCAGCUG 23 10965

HSV2-UL19-6583 - CACGUCGAGGCCCUGGCCCAGCUG 24 10966

HSV2-UL19-6584 - CGCGUGUACGCCACGCUG 18 10967

HSV2-UL19-6585 - CCGCGUGUACGCCACGCUG 19 10968

HSV2-UL19-2142 - ACCGCGUGUACGCCACGCUG 20 2441

HSV2-UL19-6586 - GACCGCGUGUACGCCACGCUG 21 10969

HSV2-UL19-6587 - CGACCGCGUGUACGCCACGCUG 22 10970

HSV2-UL19-6588 - UCGACCGCGUGUACGCCACGCUG 23 10971

HSV2-UL19-6589 - UUCGACCGCGUGUACGCCACGCUG 24 10972

HSV2-UL19-6590 - CAGAUGCUGCACGUGCUG 18 10973

HSV2-UL19-6591 - CCAGAUGCUGCACGUGCUG 19 10974

HSV2-UL19-1998 - ACCAGAUGCUGCACGUGCUG 20 2344

HSV2-UL19-6592 - GACCAGAUGCUGCACGUGCUG 21 10975

HSV2-UL19-6593 - CGACCAGAUGCUGCACGUGCUG 22 10976 HSV2-UL19-6594 - CCGACCAGAUGCUGCACGUGCUG 23 10977

HSV2-UL19-6595 - GCCGACCAGAUGCUGCACGUGCUG 24 10978

HSV2-UL19-6596 - GUGAUGGGCAAGGCCGUG 18 10979

HSV2-UL19-6597 - GGUGAUGGGCAAGGCCGUG 19 10980

HSV2-UL19-394 - UGGUGAUGGGCAAGGCCGUG 20 634

HSV2-UL19-6598 - CUGGUGAUGGGCAAGGCCGUG 21 10981

HSV2-UL19-6599 - GCUGGUGAUGGGCAAGGCCGUG 22 10982

HSV2-UL19-6600 - CGCUGGUGAUGGGCAAGGCCGUG 23 10983

HSV2-UL19-6601 - GCGCUGGUGAUGGGCAAGGCCGUG 24 10984

HSV2-UL19-6602 - CUGCUGCCGCCCCUCGUG 18 10985

HSV2-UL19-6603 - GCUGCUGCCGCCCCUCGUG 19 10986

HSV2-UL19-527 - CGCUGCUGCCGCCCCUCGUG 20 767

HSV2-UL19-6604 - GCGCUGCUGCCGCCCCUCGUG 21 10987

HSV2-UL19-6605 - GGCGCUGCUGCCGCCCCUCGUG 22 10988

HSV2-UL19-6606 - CGGCGCUGCUGCCGCCCCUCGUG 23 10989

HSV2-UL19-6607 - CCGGCGCUGCUGCCGCCCCUCGUG 24 10990

HSV2-UL19-6608 - CUGCGCAACGCGGUCGUG 18 10991

HSV2-UL19-6609 - CCUGCGCAACGCGGUCGUG 19 10992

HSV2-UL19-2199 - ACCUGCGCAACGCGGUCGUG 20 2481

HSV2-UL19-6610 - UACCUGCGCAACGCGGUCGUG 21 10993

HSV2-UL19-6611 - GUACCUGCGCAACGCGGUCGUG 22 10994

HSV2-UL19-6612 - UGUACCUGCGCAACGCGGUCGUG 23 10995

HSV2-UL19-6613 - GUGUACCUGCGCAACGCGGUCGUG 24 10996

HSV2-UL19-6614 - CAGUCCUUCCUGAAGGUG 18 10997

HSV2-UL19-6615 - GCAGUCCUUCCUGAAGGUG 19 10998

HSV2-UL19-2020 - UGCAGUCCUUCCUGAAGGUG 20 2362

HSV2-UL19-6616 - CUGCAGUCCUUCCUGAAGGUG 21 10999

HSV2-UL19-6617 - CCUGCAGUCCUUCCUGAAGGUG 22 11000

HSV2-UL19-6618 - UCCUGCAGUCCUUCCUGAAGGUG 23 11001

HSV2-UL19-6619 - CUCCUGCAGUCCUUCCUGAAGGUG 24 11002

HSV2-UL19-6620 - CUCGUCACGGCGCUGGUG 18 11003

HSV2-UL19-6621 - CCUCGUCACGGCGCUGGUG 19 11004

HSV2-UL19-2026 - ACCUCGUCACGGCGCUGGUG 20 2367

HSV2-UL19-6622 - AACCUCGUCACGGCGCUGGUG 21 11005

HSV2-UL19-6623 - CAACCUCGUCACGGCGCUGGUG 22 11006

HSV2-UL19-6624 - CCAACCUCGUCACGGCGCUGGUG 23 11007

HSV2-UL19-6625 - GCCAACCUCGUCACGGCGCUGGUG 24 11008

HSV2-UL19-6626 - AUGCUGCACGUGCUGUUG 18 11009

HSV2-UL19-6627 - GAUGCUGCACGUGCUGUUG 19 11010

HSV2-UL19-2000 - AGAUGCUGCACGUGCUGUUG 20 2345

HSV2-UL19-6628 - CAGAUGCUGCACGUGCUGUUG 21 11011

HSV2-UL19-6629 - CCAGAUGCUGCACGUGCUGUUG 22 11012

HSV2-UL19-6630 - ACCAGAUGCUGCACGUGCUGUUG 23 11013

HSV2-UL19-6631 - GACCAGAUGCUGCACGUGCUGUUG 24 11014

HSV2-UL19-6632 - CCGGCAGCUGUUUUUUUG 18 11015

HSV2-UL19-6633 - CCCGGCAGCUGUUUUUUUG 19 11016 HSV2-UL19-432 - CCCCGGCAGCUGUUUUUUUG 20 672

HSV2-UL19-6634 - UCCCCGGCAGCUGUUUUUUUG 21 11017

HSV2-UL19-6635 - CUCCCCGGCAGCUGUUUUUUUG 22 11018

HSV2-UL19-6636 - CCUCCCCGGCAGCUGUUUUUUUG 23 11019

HSV2-UL19-6637 - CCCUCCCCGGCAGCUGUUUUUUUG 24 11020

HSV2-UL19-6638 - CUACGCAGCGCCCGCGAU 18 11021

HSV2-UL19-6639 - GCUACGCAGCGCCCGCGAU 19 11022

HSV2-UL19-718 - UGCUACGCAGCGCCCGCGAU 20 958

HSV2-UL19-6640 - CUGCUACGCAGCGCCCGCGAU 21 11023

HSV2-UL19-6641 - CCUGCUACGCAGCGCCCGCGAU 22 11024

HSV2-UL19-6642 - CCCUGCUACGCAGCGCCCGCGAU 23 11025

HSV2-UL19-6643 - GCCCUGCUACGCAGCGCCCGCGAU 24 11026

HSV2-UL19-6644 - GAGUGCAUGGCCGUGUAU 18 11027

HSV2-UL19-6645 - GGAGUGCAUGGCCGUGUAU 19 11028

HSV2-UL19-509 - AGGAGUGCAUGGCCGUGUAU 20 749

HSV2-UL19-6646 - GAGGAGUGCAUGGCCGUGUAU 21 11029

HSV2-UL19-6647 - CGAGGAGUGCAUGGCCGUGUAU 22 11030

HSV2-UL19-6648 - CCGAGGAGUGCAUGGCCGUGUAU 23 11031

HSV2-UL19-6649 - CCCGAGGAGUGCAUGGCCGUGUAU 24 11032

HSV2-UL19-6650 - GUACAUCGUGACCUACCU 18 11033

HSV2-UL19-6651 - CGUACAUCGUGACCUACCU 19 11034

HSV2-UL19-503 - UCGUACAUCGUGACCUACCU 20 743

HSV2-UL19-6652 - CUCGUACAUCGUGACCUACCU 21 11035

HSV2-UL19-6653 - UCUCGUACAUCGUGACCUACCU 22 11036

HSV2-UL19-6654 - GUCUCGUACAUCGUGACCUACCU 23 11037

HSV2-UL19-6655 - GGUCUCGUACAUCGUGACCUACCU 24 11038

HSV2-UL19-6656 - CCUGGUCCCCCCGGCCCU 18 11039

HSV2-UL19-6657 - CCCUGGUCCCCCCGGCCCU 19 11040

HSV2-UL19-596 - CCCCUGGUCCCCCCGGCCCU 20 836

HSV2-UL19-6658 - CCCCCUGGUCCCCCCGGCCCU 21 11041

HSV2-UL19-6659 - UCCCCCUGGUCCCCCCGGCCCU 22 11042

HSV2-UL19-6660 - GUCCCCCUGGUCCCCCCGGCCCU 23 11043

HSV2-UL19-6661 - CGUCCCCCUGGUCCCCCCGGCCCU 24 11044

HSV2-UL19-3591 - CAACG UCCAGGCCGUCCU 18 7974

HSV2-UL19-3592 - GCAACGUCCAGGCCGUCCU 19 7975

HSV2-UL19-3085 - CGCAACGUCCAGGCCGUCCU 20 7468

HSV2-UL19-3593 - GCGCAACGUCCAGGCCGUCCU 21 7976

HSV2-UL19-3594 - CGCGCAACGUCCAGGCCGUCCU 22 7977

HSV2-UL19-3595 - GCGCGCAACGUCCAGGCCGUCCU 23 7978

HSV2-UL19-3596 - CGCGCGCAACGUCCAGGCCGUCCU 24 7979

HSV2-UL19-6662 - GACAACGCCAACCUGGCU 18 11045

HSV2-UL19-6663 - CGACAACGCCAACCUGGCU 19 11046

HSV2-UL19-2087 - CCGACAACGCCAACCUGGCU 20 2407

HSV2-UL19-6664 - CCCGACAACGCCAACCUGGCU 21 11047

HSV2-UL19-6665 - GCCCGACAACGCCAACCUGGCU 22 11048

HSV2-UL19-6666 - AGCCCGACAACGCCAACCUGGCU 23 11049 HSV2-UL19-6667 - CAG CCCG ACAACG CCAACCU G G CU 24 11050

HSV2-UL19-6668 - GGUGAUGGGCAAGGCCGU 18 11051

HSV2-UL19-6669 - UGGUGAUGGGCAAGGCCGU 19 11052

HSV2-UL19-2027 - CUGGUGAUGGGCAAGGCCGU 20 2368

HSV2-UL19-6670 - GCUGGUGAUGGGCAAGGCCGU 21 11053

HSV2-UL19-6671 - CGCUGGUGAUGGGCAAGGCCGU 22 11054

HSV2-UL19-6672 - GCGCUGGUGAUGGGCAAGGCCGU 23 11055

HSV2-UL19-6673 - GGCGCUGGUGAUGGGCAAGGCCGU 24 11056

HSV2-UL19-6674 - GAACAUCGACGCGGCCGU 18 11057

HSV2-UL19-6675 - UGAACAUCGACGCGGCCGU 19 11058

HSV2-UL19-441 - AUGAACAUCGACGCGGCCGU 20 681

HSV2-UL19-6676 - CAUGAACAUCGACGCGGCCGU 21 11059

HSV2-UL19-6677 - UCAUGAACAUCGACGCGGCCGU 22 11060

HSV2-UL19-6678 - CUCAUGAACAUCGACGCGGCCGU 23 11061

HSV2-UL19-6679 - GCUCAUGAACAUCGACGCGGCCGU 24 11062

HSV2-UL19-6680 - UCGAGGCCGCGAAUCCGU 18 11063

HSV2-UL19-6681 - GUCGAGGCCGCGAAUCCGU 19 11064

HSV2-UL19-2078 - CGUCGAGGCCGCGAAUCCGU 20 2400

HSV2-UL19-6682 - CCGUCGAGGCCGCGAAUCCGU 21 11065

HSV2-UL19-6683 - CCCGUCGAGGCCGCGAAUCCGU 22 11066

HSV2-UL19-6684 - CCCCGUCGAGGCCGCGAAUCCGU 23 11067

HSV2-UL19-6685 - ACCCCGUCGAGGCCGCGAAUCCGU 24 11068

HSV2-UL19-6686 - UGU UUUCCGAGCGCGCGU 18 11069

HSV2-UL19-6687 - CUGUU UUCCGAGCGCGCGU 19 11070

HSV2-UL19-618 - GCUGUUUUCCGAGCGCGCGU 20 858

HSV2-UL19-6688 - UGCUGUUUUCCGAGCGCGCGU 21 11071

HSV2-UL19-6689 - GUGCUGUUUUCCGAGCGCGCGU 22 11072

HSV2-UL19-6690 - CGUGCUGUUUUCCGAGCGCGCGU 23 11073

HSV2-UL19-6691 - ACGUGCUGUUUUCCGAGCGCGCGU 24 11074

HSV2-UL19-6692 - CCGCCGUCCGCGGGGCGU 18 11075

HSV2-UL19-6693 - GCCGCCGUCCGCGGGGCGU 19 11076

HSV2-UL19-2101 - AGCCGCCGUCCGCGGGGCGU 20 2415

HSV2-UL19-6694 - UAGCCGCCGUCCGCGGGGCGU 21 11077

HSV2-UL19-6695 - AUAGCCGCCGUCCGCGGGGCGU 22 11078

HSV2-UL19-6696 - CAUAGCCGCCGUCCGCGGGGCGU 23 11079

HSV2-UL19-6697 - CCAUAGCCGCCGUCCGCGGGGCGU 24 11080

HSV2-UL19-6698 - AGGCCGUCCUGGGGGCGU 18 11081

HSV2-UL19-6699 - CAGGCCGUCCUGGGGGCGU 19 11082

HSV2-UL19-1997 - CCAGGCCGUCCUGGGGGCGU 20 2343

HSV2-UL19-6700 - UCCAGGCCGUCCUGGGGGCGU 21 11083

HSV2-UL19-6701 - GUCCAGGCCGUCCUGGGGGCGU 22 11084

HSV2-UL19-6702 - CGUCCAGGCCGUCCUGGGGGCGU 23 11085

HSV2-UL19-6703 - ACGUCCAGGCCGUCCUGGGGGCGU 24 11086

HSV2-UL19-6704 - GCUGCUGCCGCCCCUCGU 18 11087

HSV2-UL19-6705 - CGCUGCUGCCGCCCCUCGU 19 11088

HSV2-UL19-2124 - GCGCUGCUGCCGCCCCUCGU 20 2432 HSV2-UL19-6706 - GGCGCUGCUGCCGCCCCUCGU 21 11089

HSV2-UL19-6707 - CGGCGCUGCUGCCGCCCCUCGU 22 11090

HSV2-UL19-6708 - CCGGCGCUGCUGCCGCCCCUCGU 23 11091

HSV2-UL19-6709 - CCCGGCGCUGCUGCCGCCCCUCGU 24 11092

HSV2-UL19-6710 - CGUCGCAGCGGUUCUCGU 18 11093

HSV2-UL19-6711 - GCGUCGCAGCGGUUCUCGU 19 11094

HSV2-UL19-2224 - GGCGUCGCAGCGGUUCUCGU 20 2494

HSV2-UL19-6712 - GGGCGUCGCAGCGGUUCUCGU 21 11095

HSV2-UL19-6713 - UGGGCGUCGCAGCGGUUCUCGU 22 11096

HSV2-UL19-6714 - GUGGGCGUCGCAGCGGUUCUCGU 23 11097

HSV2-UL19-6715 - CGUGGGCGUCGCAGCGGUUCUCGU 24 11098

HSV2-UL19-6716 - CCGGAGAUCGCCCCCGGU 18 11099

HSV2-UL19-6717 - CCCGGAGAUCGCCCCCGGU 19 11100

HSV2-UL19-2146 - UCCCGGAGAUCGCCCCCGGU 20 2444

HSV2-UL19-6718 - GUCCCGGAGAUCGCCCCCGGU 21 11101

HSV2-UL19-6719 - GGUCCCGGAGAUCGCCCCCGGU 22 11102

HSV2-UL19-6720 - UGGUCCCGGAGAUCGCCCCCGGU 23 11103

HSV2-UL19-6721 - GUGGUCCCGGAGAUCGCCCCCGGU 24 11104

HSV2-UL19-6722 - GAAGUCCCCCCGGCCGGU 18 11105

HSV2-UL19-6723 - CGAAG UCCCCCCGGCCGG U 19 11106

HSV2-UL19-2092 - GCGAAGUCCCCCCGGCCGGU 20 2411

HSV2-UL19-6724 - GGCGAAGUCCCCCCGGCCGGU 21 11107

HSV2-UL19-6725 - CGGCGAAGUCCCCCCGGCCGGU 22 11108

HSV2-UL19-6726 - GCGGCGAAGUCCCCCCGGCCGGU 23 11109

HSV2-UL19-6727 - GGCGGCGAAGUCCCCCCGGCCGGU 24 11110

HSV2-UL19-6728 - G U CCAU GG AG AACG CG G U 18 11111

HSV2-UL19-6729 - UGUCCAUGGAGAACGCGGU 19 11112

HSV2-UL19-438 - CUGUCCAUGGAGAACGCGGU 20 678

HSV2-UL19-6730 - GCUGUCCAUGGAGAACGCGGU 21 11113

HSV2-UL19-6731 - GGCUGUCCAUGGAGAACGCGGU 22 11114

HSV2-UL19-6732 - CGGCUGUCCAUGGAGAACGCGGU 23 11115

HSV2-UL19-6733 - GCGGCUGUCCAUGGAGAACGCGGU 24 11116

HSV2-UL19-6734 - GGAAACGUGGACCUGGGU 18 11117

HSV2-UL19-6735 - CGGAAACGUGGACCUGGGU 19 11118

HSV2-UL19-2193 - GCGGAAACGUGGACCUGGGU 20 2476

HSV2-UL19-6736 - CGCGGAAACGUGGACCUGGGU 21 11119

HSV2-UL19-6737 - GCGCGGAAACGUGGACCUGGGU 22 11120

HSV2-UL19-6738 - CGCGCGGAAACGUGGACCUGGGU 23 11121

HSV2-UL19-6739 - CCGCGCGGAAACGUGGACCUGGGU 24 11122

HSV2-UL19-6740 - AGAUGCUGCACGUGCUGU 18 11123

HSV2-UL19-6741 - CAGAUGCUGCACGUGCUGU 19 11124

HSV2-UL19-348 - CCAGAUGCUGCACGUGCUGU 20 588

HSV2-UL19-6742 - ACCAGAUGCUGCACGUGCUGU 21 11125

HSV2-UL19-6743 - GACCAGAUGCUGCACGUGCUGU 22 11126

HSV2-UL19-6744 - CGACCAGAUGCUGCACGUGCUGU 23 11127

HSV2-UL19-6745 - CCGACCAGAUGCUGCACGUGCUGU 24 11128 HSV2-UL19-6746 - ACCGUCCG CAACCCCG U U 18 11129

HSV2-UL19-6747 - CACCGUCCGCAACCCCGUU 19 11130

HSV2-UL19-2195 - GCACCGUCCGCAACCCCGUU 20 2478

HSV2-UL19-6748 - GGCACCG UCCGCAACCCCGU U 21 11131

HSV2-UL19-6749 - GGGCACCGUCCGCAACCCCGUU 22 11132

HSV2-UL19-6750 - CGGGCACCGUCCG CAACCCCG U U 23 11133

HSV2-UL19-6751 - ACGGGCACCGUCCGCAACCCCGUU 24 11134

HSV2-UL19-6752 - GCGUCGGAGGCGUACUUU 18 11135

HSV2-UL19-6753 - CGCGUCGGAGGCGUACUUU 19 11136

HSV2-UL19-2181 - GCGCGUCGGAGGCGUACUUU 20 2469

HSV2-UL19-6754 - CGCGCGUCGGAGGCGUACUUU 21 11137

HSV2-UL19-6755 - GCGCGCGUCGGAGGCGUACUUU 22 11138

HSV2-UL19-6756 - AGCGCGCGUCGGAGGCGUACUUU 23 11139

HSV2-UL19-6757 - GAGCGCGCGUCGGAGGCGUACUUU 24 11140

HSV2-UL19-6758 - GACCCGUGCGGCCUGUUU 18 11141

HSV2-UL19-6759 - AGACCCGUGCGGCCUGUUU 19 11142

HSV2-UL19-2242 - AAGACCCGUGCGGCCUGUUU 20 2503

HSV2-UL19-6760 - GAAGACCCGUGCGGCCUGUUU 21 11143

HSV2-UL19-6761 - GGAAGACCCGUGCGGCCUGUUU 22 11144

HSV2-UL19-6762 - UGGAAGACCCGUGCGGCCUGUUU 23 11145

HSV2-UL19-6763 - GUGGAAGACCCGUGCGGCCUGUUU 24 11146

HSV2-UL19-6764 - CCCGUCCACGCGCUGUUU 18 11147

HSV2-UL19-6765 - GCCCGUCCACGCGCUGUUU 19 11148

HSV2-UL19-2157 - UGCCCGUCCACGCGCUGUUU 20 2453

HSV2-UL19-6766 - CUGCCCGUCCACGCGCUGUUU 21 11149

HSV2-UL19-6767 - CCUGCCCGUCCACGCGCUGUUU 22 11150

HSV2-UL19-6768 - UCCUGCCCGUCCACGCGCUGUUU 23 11151

HSV2-UL19-6769 - GUCCUGCCCGUCCACGCGCUGUUU 24 11152

HSV2-UL19-6770 - GACAUGCAGCAGCGUUUU 18 11153

HSV2-UL19-6771 - GGACAUGCAGCAGCGUUUU 19 11154

HSV2-UL19-2081 - CGGACAUGCAGCAGCGUUUU 20 2402

HSV2-UL19-6772 - GCGGACAUGCAGCAGCGUUUU 21 11155

HSV2-UL19-6773 - CGCGGACAUGCAGCAGCGUUUU 22 11156

HSV2-UL19-6774 - GCGCGGACAUGCAGCAGCGUUUU 23 11157

HSV2-UL19-6775 - GGCGCGGACAUGCAGCAGCGUUUU 24 11158

HSV2-UL19-6776 - CCGAGAACGUGCUGUUUU 18 11159

HSV2-UL19-6777 - ACCGAGAACGUGCUGUUUU 19 11160

HSV2-UL19-2178 - GACCGAGAACGUGCUGUUUU 20 2467

HSV2-UL19-6778 - UGACCGAGAACGUGCUGUUUU 21 11161

HSV2-UL19-6779 - GUGACCGAGAACGUGCUGUUUU 22 11162

HSV2-UL19-6780 - CGUGACCGAGAACGUGCUGUUUU 23 11163

HSV2-UL19-6781 - UCGUGACCGAGAACGUGCUGUUUU 24 11164

HSV2-UL19-6782 - UCCCCGGCAGCUGUUUUU 18 11165

HSV2-UL19-6783 - CUCCCCGGCAGCUGUUUUU 19 11166

HSV2-UL19-2061 - CCUCCCCGGCAGCUGUUUUU 20 2390

HSV2-UL19-6784 - CCCUCCCCGGCAGCUGUUUUU 21 11167 HSV2-UL19-6785 - UCCCUCCCCGGCAGCUGUUUUU 22 11168

HSV2-UL19-6786 - UUCCCUCCCCGGCAGCUGUUUUU 23 11169

HSV2-UL19-6787 - GUUCCCUCCCCGGCAGCUGUUUUU 24 11170

HSV2-UL19-6788 - CCCCGGCAGCUGUUUUUU 18 11171

HSV2-UL19-6789 - UCCCCGGCAGCUGUUUUUU 19 11172

HSV2-UL19-430 - CUCCCCGGCAGCUGUUUUUU 20 670

HSV2-UL19-6790 - CCUCCCCGGCAGCUGUUUUUU 21 11173

HSV2-UL19-6791 - CCCUCCCCGGCAGCUGUUUUUU 22 11174

HSV2-UL19-6792 - UCCCUCCCCGGCAGCUGUUUUUU 23 11175

HSV2-UL19-6793 - UUCCCUCCCCGGCAGCUGUUUUUU 24 11176

HSV2-UL19-6794 - CCCGGCAGCUGUUUUUUU 18 11177

HSV2-UL19-6795 - CCCCGGCAGCUGUUUUUUU 19 11178

HSV2-UL19-431 - UCCCCGGCAGCUGUUUUUUU 20 671

HSV2-UL19-6796 - CUCCCCGGCAGCUGUUUUUUU 21 11179

HSV2-UL19-6797 - CCUCCCCGGCAGCUGUUUUUUU 22 11180

HSV2-UL19-6798 - CCCUCCCCGGCAGCUGUUUUUUU 23 11181

HSV2-UL19-6799 - UCCCUCCCCGGCAGCUGUUUUUUU 24 11182

Table 7 A provides exemplary targeting domains for knocking out the UL19 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 7A

Table 7B provides exemplary targeting domains for knocking out the UL19 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase)

Table 7B

Table 7C provides exemplary targeting domains for knocking out the UL19 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 7C

HSV2-UL19-6812 - UGU UCAACCCGGCCAUG 17 11195

HSV2-UL19-6813 + UGCUCGUUGCCGUGAAU 17 11196

HSV2-UL19-3068 + CGGCACCAGCACGUAAU 17 3065

HSV2-UL19-3062 - GGACCGCCACCGAGACU 17 3060

HSV2-UL19-6814 - AAAGGGCCUGUCUCUGUAAA 20 11197

HSV2-UL19-6815 + CGACG CG CCCG U U G U G G AAC 20 11198

HSV2-UL19-2008 - UGAAGCGGAGCUU UUGCGAC 20 2352

HSV2-UL19-3063 + UGCGCUUGACCGUGU UGGCC 20 3061

HSV2-UL19-6816 - GGGCCUCGCCCGUCCUCAGC 20 11199

HSV2-UL19-1127 + CGAGCUCGCGGCACCCCGGC 20 1367

HSV2-UL19-1353 + GCAGCGCCGGGUCGCGCAUC 20 1593

HSV2-UL19-6817 - GUAAAAUUGGCUUCU UCUUC 20 11200

HSV2-UL19-6818 + AGAAGCUCGUGUCGCAAAAG 20 11201

HSV2-UL19-3059 - GGUGUUUUACCUGCUGCAAG 20 3057

HSV2-UL19-359 - CCCUGGUCGCCGAGCUGAAG 20 599

HSV2-UL19-6819 - GGUUCACCGUCGUGCGGCAG 20 11202

HSV2-UL19-3066 + GGGCCGCGACG UACGCCCCG 20 3063

HSV2-UL19-6820 + UCGCUGGCGCAGGUGAUCGG 20 11203

HSV2-UL19-6821 - GGCGCUGCUGCACCGCGGGG 20 11204

HSV2-UL19-1492 + GCGCACGCGCGUUGUCUGGG 20 1732

HSV2-UL19-6822 - GGCUGUUCAACCCGGCCAUG 20 11205

HSV2-UL19-3064 + GGCCGGCACCAGCACG UAAU 20 3062

HSV2-UL19-3060 - CCUGGACCGCCACCGAGACU 20 3058

Table 8A provides exemplary targeting domains for knocking out the UL30 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 8A

HSV2-U L30-217 + GCGUUCCGGUCUGAGCG 17 3148

HSV2-U L30-162 - GCGCGACGUCCUCCGCG 17 3121

HSV2-U L30-207 + GGUGCGCUGCUCCGCGG 17 3142

HSV2-U L30-188 + GGGGUCGAACCCCUCGG 17 3134

HSV2-UL30-156 - GCCCCUAAGGUGUACUG 17 3116

HSV2-UL30-169 - GCGUCGCU UGCGCCUGU 17 3124

HSV2-UL30-66 + GGUCGAACCCCUCGGGGGCA 20 3090

HSV2-UL30-51 - GGUGCGGACCAUGCCCCCGA 20 3083

HSV2-UL30-58 + GUACACGUGGAAGACGGUGA 20 3196

HSV2-UL30-27 - GCCCCCGCGGAGCAGCGCAC 20 3180

HSV2-UL30-2855 - GCGUAUAGCAGGACAACGAC 20 11209

HSV2-U L30-122 + GCUUCCCCCCGGGGGAAGCC 20 3233

HSV2-UL30-1 - GUU UUGUGCCGCGGGCGGCC 20 3157

HSV2-UL30-70 + GACGCGGCCAGAAGCCCUCC 20 3200

HSV2-UL30-7 - GGCUUCCCCCGGGGGGAAGC 20 3163

HSV2-UL30-2856 + GCGGCACAAAACAUCGCCGC 20 11210

HSV2-UL30-2857 - GCGGCGAUGUU UUGUGCCGC 20 11211

HSV2-UL30-34 - GCGCCCCUAAGGUGUACUGC 20 3072

HSV2-UL30-31 - GGGUCCACGACGGCCGCCUC 20 3070

HSV2-U L30-104 + GGCAAGGCGGCGGUGCCGUC 20 3109

HSV2-UL30-85 + GCGUCCUCGUCCAGCGAACG 20 3098

HSV2-UL30-2858 - GGCGGCGAUGU UUUGUGCCG 20 11212

HSV2-UL30-94 + GCUGCGUUCCGGUCUGAGCG 20 3103

HSV2-UL30-87 + GUCCUCG UCCAGCGAACGCG 20 3210

HSV2-UL30-95 + GCGUUCCGGUCUGAGCGAGG 20 3104

HSV2-UL30-36 - GCCCCUAAGGUGUACUGCGG 20 3073

HSV2-UL30-65 + GGUGGGGUCGAACCCCUCGG 20 3089

HSV2-UL30-2859 - GCGAUGUU UUGUGCCGCGGG 20 11213

HSV2-UL30-97 + GU UCCGGUCUGAGCGAGGUG 20 3105

HSV2-UL30-62 + GACGGUGGGGUCGAACCCCU 20 3086

HSV2-UL30-105 + GCAAGGCGGCGGUGCCGUCU 20 3110

HSV2-UL30-40 - GAGCGCGACGUCCUCCGCGU 20 3077

HSV2-UL30-46 - GCCGCGUCGCU UGCGCCUGU 20 3079

HSV2-U L30-112 + GGUGGCUCCCCGGGGGUUGU 20 3223

Table 8B provides exemplary targeting domains for knocking out the UL30 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 8B

HSV2-U L30-118 + CGAGCCGCCGGCUUCCCCCC 20 3229

HSV2-U L30-117 + CCGAGCCGCCGGCUUCCCCC 20 3228

HSV2-UL30-41 - CGACGUCCUCCGCGUGGGCC 20 3186

HSV2-UL30-53 - CUUCCACGUGUACGACAUCC 20 3084

HSV2-UL30-55 + ACGCGUGUUCCACGUGCUCC 20 3193

HSV2-UL30-2862 + CCAUAAAUCGCUCGUGGAGC 20 11216

HSV2-UL30-89 + UAGUACGUAUGGCGCUGAGC 20 3100

HSV2-UL30-86 + CGUCCUCGUCCAGCGAACGC 20 3099

HSV2-UL30-18 - CGGCACCGCCGCCUUGCCGC 20 3067

HSV2-UL30-76 + AGUACACCU UAGGGGCGCGC 20 3095

HSV2-UL30-24 - AUUUAUCGCCCCGCGUUCGC 20 3178

HSV2-UL30-63 + ACGGUGGGGUCGAACCCCUC 20 3087

HSV2-UL30-69 + CGACGCGGCCAGAAGCCCUC 20 3092

HSV2-UL30-10 - CGGGGGGAAGCCGGCGGCUC 20 3166

HSV2-UL30-75 + CCCCCCGCAG U ACACCU U AG 20 3094

HSV2-UL30-68 + CCCCACAGGCGCAAGCGACG 20 3091

HSV2-UL30-25 - CGCCCCGCGUUCGCUGGACG 20 3069

HSV2-UL30-29 - CCCCGCGGAGCAGCGCACCG 20 3182

HSV2-UL30-50 - CGGUGCGGACCAUGCCCCCG 20 3082

HSV2-UL30-90 + UAUGGCGCUGAGCCGGCCCG 20 3212

HSV2-UL30-39 - CGAGCGCGACGUCCUCCGCG 20 3076

HSV2-UL30-35 - CGCCCCUAAGGUGUACUGCG 20 3185

HSV2-UL30-64 + CGGUGGGGUCGAACCCCUCG 20 3088

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-102 + AAGUUCUGCCGGCGGCAAGG 20 3108

HSV2-UL30-78 + CCUUAGGGGCGCGCCGGAGG 20 3204

HSV2-UL30-2863 - UAUAGCAGGACAACGACCGG 20 11217

HSV2-UL30-110 + CCGUCUGGGUGGCUCCCCGG 20 3111

HSV2-UL30-77 + ACACCU UAGGGGCGCGCCGG 20 3096

HSV2-UL30-84 + CCCGGUGCGCUGCUCCGCGG 20 3097

HSV2-UL30-37 - CCCCUAAGGUGUACUGCGGG 20 3074

HSV2-UL30-38 - CCCUAAGGUGUACUGCGGGG 20 3075

HSV2-UL30-48 - CGUCGCU UGCGCCUGUGGGG 20 3081

HSV2-UL30-2864 + AAAUCGCUCGUGGAGCUGGG 20 11218

HSV2-UL30-33 - CGCGCCCCUAAGGUGUACUG 20 3071

HSV2-UL30-49 - CUUGCGCCUGUGGGGCGGUG 20 3190

HSV2-UL30-47 - CCGCGUCGCUUGCGCCUGUG 20 3080

HSV2-UL30-9 - CCGGGGGGAAGCCGGCGGCU 20 3165

HSV2-UL30-96 + CGUUCCGGUCUGAGCGAGGU 20 3214

HSV2-UL30-60 + ACGUGGAAGACGGUGACGGU 20 3085

Table 8C provides exemplary targeting domains for knocking out the UL30 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 8C

HSV2-UL30-121 + GGCU UCCCCCCGGGGG AAGC 20 3232

HSV2-UL30-23 - G C AG CCA A AG GCCCUCGGGC 20 3177

HSV2-UL30-99 + GGGGGUUGUAGAAGUUCUGC 20 3216

HSV2-UL30-22 - GAACGCAGCCAAAGGCCCUC 20 3176

HSV2-UL30-44 - GCGUGGGCCCGGAGGGCUUC 20 3188

HSV2-UL30-93 + GAGGGCCUUUGGCUGCGUUC 20 3102

HSV2-UL30-52 - GUGCGGACCAUGCCCCCGAG 20 3191

HSV2-UL30-56 + GCUCCAGGAUGUCGUACACG 20 3194

HSV2-UL30-54 - GUACGACAUCCUGGAGCACG 20 3192

HSV2-UL30-119 + GAGCCGCCGGCUUCCCCCCG 20 3230

HSV2-UL30-26 - GCUGGACGAGGACGCCCCCG 20 3179

HSV2-UL30-4 - GGGCGGCCCGGCU UCCCCCG 20 3160

HSV2-UL30-109 + GCCGUCUGGGUGGCUCCCCG 20 3221

HSV2-UL30-123 + GGGGGAAGCCGGGCCGCCCG 20 3234

HSV2-UL30-81 + GACCCCGGUGCGCUGCUCCG 20 3207

HSV2-UL30-5 - GGCGGCCCGGCUUCCCCCGG 20 3161

HSV2-UL30-100 + GGUUGUAGAAGUUCUGCCGG 20 3106

HSV2-UL30-6 - GCGGCCCGGCUUCCCCCGGG 20 3162

HSV2-UL30-11 - GGGGAAGCCGGCGGCUCGGG 20 3167

HSV2-UL30-115 + GGCUCCCCGGGGGUUGUGGG 20 3226

HSV2-UL30-45 - GGCCGCGUCGCU UGCGCCUG 20 3189

HSV2-UL30-113 + GUGGCUCCCCGGGGGUUGUG 20 3224

HSV2-UL30-111 + GGGUGGCUCCCCGGGGGU UG 20 3222

HSV2-UL30-21 - GGAACGCAGCCAAAGGCCCU 20 3175

Table 8D provides exemplary targeting domains for knocking out the UL30 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 8D

HSV2-U L30-150 - CCCGCGGAGCAGCGCAC 17 3258

HSV2-U L30-142 - CCCCACCUCGCUCAGAC 17 3252

HSV2-UL30-2866 - UAUAGCAGGACAACGAC 17 11220

HSV2-U L30-151 - CCGCGGAGCAGCGCACC 17 3259

HSV2-UL30-137 - UUGCCCCCCACAACCCC 17 3248

HSV2-UL30-138 - UGCCCCCCACAACCCCC 17 3249

HSV2-U L30-240 + AGCCGCCGGCUUCCCCC 17 3306

HSV2-UL30-231 + CGUCUGGGUGGCUCCCC 17 3298

HSV2-UL30-230 + CCGUCUGGGUGGCUCCC 17 3297

HSV2-U L30-245 + UCCCCCCGGGGGAAGCC 17 3311

HSV2-UL30-124 - UUGUGCCGCGGGCGGCC 17 3235

HSV2-U L30-164 - CGUCCUCCGCGUGGGCC 17 3264

HSV2-U L30-176 - CCACG UG U ACG ACAU CC 17 3129

HSV2-UL30-178 + CGUGU UCCACGUGCUCC 17 3271

HSV2-U L30-244 + U UCCCCCCGGGGG AAGC 17 3310

HSV2-UL30-130 - UUCCCCCGGGGGGAAGC 17 3241

HSV2-UL30-141 - CACCGCCGCCUUGCCGC 17 3112

HSV2-U L30-157 - CCCCUAAGGUGUACUGC 17 3117

HSV2-UL30-192 + CGCGGCCAGAAGCCCUC 17 3137

HSV2-U L30-145 - CG C AG CCA A AG G CCC U C 17 3254

HSV2-U L30-154 - UCCACGACGGCCGCCUC 17 3115

HSV2-UL30-167 - UGGGCCCGGAGGGCUUC 17 3266

HSV2-U L30-198 + CCCGCAGUACACCUUAG 17 3139

HSV2-U L30-179 + CCAGGAUGUCGUACACG 17 3272

HSV2-UL30-194 + AG CCC U CCG G G CCC ACG 17 3279

HSV2-U L30-177 - CGACAUCCUGGAGCACG 17 3270

HSV2-UL30-242 + CCGCCGGCUUCCCCCCG 17 3308

HSV2-UL30-127 - CGGCCCGGCUUCCCCCG 17 3238

HSV2-U L30-204 + CCCGGUGCGCUGCUCCG 17 3285

HSV2-UL30-225 + UUCUGCCGGCGGCAAGG 17 3153

HSV2-UL30-218 + UUCCGGUCUGAGCGAGG 17 3149

HSV2-UL30-2867 - AGCAGGACAACGACCGG 17 11221

HSV2-UL30-243 + CGCCGGCUUCCCCCCGG 17 3309

HSV2-U L30-233 + UCUGGGUGGCUCCCCGG 17 3156

HSV2-U L30-165 - CCUCCGCGUGGGCCCGG 17 3265

HSV2-UL30-131 - CCCCGGGGGGAAGCCGG 17 3242

HSV2-U L30-223 + UGUAGAAGUUCUGCCGG 17 3151

HSV2-UL30-195 + CCUCCGGGCCCACGCGG 17 3280

HSV2-UL30-226 + UGCCGGCGGCAAGGCGG 17 3295

HSV2-U L30-160 - CUAAGGUGUACUGCGGG 17 3119

HSV2-U L30-161 - UAAGG UGUACUGCGGGG 17 3120

HSV2-U L30-171 - CGCU UGCGCCUG UGGGG 17 3126

HSV2-UL30-2868 + UCGCUCGUGGAGCUGGG 17 11222

HSV2-UL30-229 + CGGCGGUGCCGUCUGGG 17 3296

HSV2-U L30-238 + UCCCCGGGGGUUG UGGG 17 3304

HSV2-U L30-221 + CGGUCUGAGCGAGGUGG 17 3293 HSV2-U L30-237 + CUCCCCGGGGGUUGUGG 17 3303

HSV2-U L30-220 + CCGGUCUGAGCGAGGUG 17 3150

HSV2-U L30-234 + UGGCUCCCCGGGGGU UG 17 3300

HSV2-U L30-144 - ACG CAG CCA AAGG CCCU 17 3253

HSV2-UL30-2869 + AAAUCGCUCGUGGAGCU 17 11223

HSV2-UL30-136 - CGGCUCGGGCGGCGUCU 17 3247

HSV2-U L30-183 + UGGAAGACGGUGACGG U 17 3130

HSV2-UL30-196 + CCCCCGCAGUACACCU U 17 3281

HSV2-UL30-30 - CAGCGCACCGGGGUCCACGA 20 3183

HSV2-UL30-2870 - CCAGCUCCACGAGCGAU UUA 20 11224

HSV2-UL30-19 - AACCCCCACCUCGCUCAGAC 20 3174

HSV2-UL30-14 - U U U U UGCCCCCCACAACCCC 20 3170

HSV2-UL30-15 - U U U U G CCCCCCACA ACCCCC 20 3171

HSV2-UL30-3 - CGGGCGGCCCGGCUUCCCCC 20 3159

HSV2-U L30-108 + UGCCGUCUGGGUGGCUCCCC 20 3220

HSV2-UL30-116 + CCAGACGCCGCCCGAGCCGC 20 3227

HSV2-UL30-82 + ACCCCGGUGCGCUGCUCCGC 20 3208

HSV2-UL30-12 - CCGGCGGCUCGGGCGGCGUC 20 3168

HSV2-UL30-71 + AGAAGCCCUCCGGGCCCACG 20 3201

HSV2-UL30-16 - U U U G CCCCCCAC AACCCCCG 20 3172

HSV2-UL30-83 + CCCCGG UGCGCUGCUCCGCG 20 3209

HSV2-UL30-79 + CGCGCCGGAGGCGGCCGUCG 20 3205

HSV2-UL30-59 + CACGUGGAAGACGGUGACGG 20 3197

HSV2-U L30-120 + AGCCGCCGGCUUCCCCCCGG 20 3231

HSV2-UL30-42 - CGUCCUCCGCGUGGGCCCGG 20 3187

HSV2-U L30-8 - UUCCCCCGGGGGGAAGCCGG 20 3164

HSV2-UL30-72 + AGCCCUCCGGGCCCACGCGG 20 3202

HSV2-UL30-103 + UUCUGCCGGCGGCAAGGCGG 20 3217

HSV2-U L30-106 + AGGCGGCGGUGCCGUCUGGG 20 3218

HSV2-UL30-98 + UUCCGGUCUGAGCGAGGUGG 20 3215

HSV2-UL30-114 + UGGCUCCCCGGGGGUUGUGG 20 3225

HSV2-UL30-61 + CGUGGAAGACGGUGACGGUG 20 3198

HSV2-UL30-2871 + CAU AAAUCGCUCG UGG AGCU 20 11225

HSV2-UL30-13 - CGGCGGCUCGGGCGGCGUCU 20 3169

HSV2-UL30-73 + UCCCCCCCGCAGUACACCU U 20 3203

HSV2-UL30-92 + UGAGCCGGCCCGAGGGCCUU 20 3213

Table 8E provides exemplary targeting domains for knocking out the UL30 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 8E

HSV2-UL30-1485 + CU UGGCCGGGGAGGGCA 17 4355

HSV2-UL30-1537 + CUGCAGUCCCUCGGGCA 17 4407

HSV2-UL30-1777 + AGAGAUCGCGCGGGGCA 17 4647

HSV2-UL30-821 - CUUCGAUAUCGAAUGCA 17 3691

HSV2-UL30-813 - CGUCGAGUUUAACUGCA 17 3683

HSV2-UL30-966 - GCAACAGGCCGCCAUCA 17 3836

HSV2-UL30-1031 - GCAGGUCCCGUCCAUCA 17 3901

HSV2-UL30-1004 - GGGCAAGAUGCUCAUCA 17 3874

HSV2-UL30-857 - GAAGCGCAGCAAGAUCA 17 3727

HSV2-UL30-991 - UU UGUGCCGCGGCCUCA 17 3861

HSV2-UL30-1029 - GGUGUAU UACAAGCUCA 17 3899

HSV2-UL30-1072 - CGUGGCCGCGCGGCUCA 17 3942

HSV2-UL30-1580 + GCGGCCGAUGGUCGUCA 17 4450

HSV2-UL30-1064 - GGCCUGCGUGACGUUCA 17 3934

HSV2-UL30-998 - GGCCAUGGGCGACAAGA 17 3868

HSV2-UL30-1673 + CUUCUUGUCCU UCAAGA 17 4543

HSV2-UL30-865 - CU UG AAGG ACAAG AAG A 17 3735

HSV2-UL30-883 - GCGCCUUGCGGGCCAGA 17 3753

HSV2-UL30-1036 - CCGCGAGGUAGAGGAGA 17 3906

HSV2-UL30-1025 - CAUCACCGACCCGGAGA 17 3895

HSV2-UL30-1656 + CUGCUGGCCGUCGUAGA 17 4526

HSV2-UL30-1695 + G U C A A AC U CCAGGACGA 17 4565

HSV2-UL30-1686 + CAGCUUGGUCAGGACGA 17 4556

HSV2-UL30-1692 + GCCGUACUGCUUGACGA 17 4562

HSV2-UL30-880 - ACCCGCACCAUCUACGA 17 3750

HSV2-UL30-796 - GCGAUCAGGAAGUACGA 17 3666

HSV2-UL30-769 - CUGGGUCUGACCCCCGA 17 3639

HSV2-UL30-811 - CCAACCGCGCCCCCCGA 17 3681

HSV2-UL30-1017 - GCGCGACCCCUGCCCGA 17 3887

HSV2-UL30-1748 + GAGGCGGUACCAGCCGA 17 4618

HSV2-UL30-1530 + GGUCGGUGAUGCGCCGA 17 4400

HSV2-UL30-1579 + GAGCAUCUCGCGGCCGA 17 4449

HSV2-UL30-958 - UCGAGGUGGGGGGCCGA 17 3828

HSV2-UL30-1780 + CGUGAACGGCGACGCGA 17 4650

HSV2-UL30-1660 + GCCCGCCAGGCGCGCGA 17 4530

HSV2-UL30-1610 + CGCCUCCAGGUGCGCGA 17 4480

HSV2-UL30-1675 + CAAGACGGCCUCGGCGA 17 4545

HSV2-UL30-1459 + CGGAACGCCCCGGGCGA 17 4329

HSV2-UL30-1473 + GGGGGGUCGGCAUGCGA 17 4343

HSV2-UL30-1731 + ACACAUGGCCCCCUCGA 17 4601

HSV2-UL30-846 - U ACAAG GUCCCGCUCGA 17 3716

HSV2-UL30-1714 + CAGGAGGAUGUGCUCGA 17 4584

HSV2-UL30-817 - GACAACCUGGCCGUCGA 17 3687

HSV2-UL30-2873 + CGGGACAUCAGCUUCGA 17 11227

HSV2-UL30-917 - AAGGACGACGACGAGGA 17 3787

HSV2-UL30-921 - GAGGACGGGGACGAGGA 17 3791 HSV2-UL30-1533 + AUGGGCGUCUACGAGGA 17 4403

HSV2-UL30-1591 + UUGCU UGUCGAGGAGGA 17 4461

HSV2-UL30-1501 + GGCGUCUAGCUCGCGGA 17 4371

HSV2-UL30-860 - GAUCAAGGUGAACGGGA 17 3730

HSV2-UL30-1483 + GGGCGCUUGGCCGGGGA 17 4353

HSV2-UL30-909 - GGGGAGCGGCCGGGGGA 17 3779

HSV2-UL30-901 - GCCGUGCCUCGGGGGGA 17 3771

HSV2-UL30-1511 + AUCCGGUCCUUGAUGGA 17 4381

HSV2-UL30-1693 + CU UGACGAAGGUCAUGA 17 4563

HSV2-UL30-1785 + ACCCAGAAGCGUGAUGA 17 4655

HSV2-UL30-1028 - GCGCCUGGCCCACCUGA 17 3898

HSV2-UL30-844 - CGUCCUGACCAAGCUGA 17 3714

HSV2-UL30-1789 + GGUCCCGGCGGGCGUGA 17 4659

HSV2-UL30-959 - ACGGCUGUUCUUCGUGA 17 3829

HSV2-UL30-1587 + GUUGCACACCACCUUGA 17 4457

HSV2-UL30-1510 + CGGGAUCCGGUCCU UGA 17 4380

HSV2-UL30-864 - CGCCGAGGCCGUCU UGA 17 3734

HSV2-UL30-915 - GAACGGGGACGAGGAUA 17 3785

HSV2-UL30-1546 + GGCGGCCGCUCCGGAUA 17 4416

HSV2-UL30-1084 - GGCGGGGGCCGGCGCUA 17 3954

HSV2-UL30-986 - UCCAUGCGCAUCAUCUA 17 3856

HSV2-UL30-770 - GUCGCCGUUCACGUCUA 17 3640

HSV2-UL30-840 - ACCUUCGU C A AG C AG U A 17 3710

HSV2-UL30-1569 + AUGAUGCGCAUGGAGUA 17 4439

HSV2-UL30-1507 + UGGGCCACGAUCACGUA 17 4377

HSV2-UL30-848 - GUCCCGCUCGACGGGUA 17 3718

HSV2-UL30-861 - GUGAACAUCGACAUGUA 17 3731

HSV2-UL30-969 - GUGUGCAACUCGGUGUA 17 3839

HSV2-UL30-2865 - GCUCCACGAGCGAUUUA 17 11219

HSV2-UL30-1711 + AGGAUCCGAGCGAAAAC 17 4581

HSV2-UL30-1452 + UGGCGU UAUUUCCAAAC 17 4322

HSV2-UL30-1752 + GGUUGUCCAGGAUAAAC 17 4622

HSV2-UL30-965 - CCUCCUCGACAAGCAAC 17 3835

HSV2-UL30-912 - GGCCGGGGGACGGGAAC 17 3782

HSV2-UL30-859 - G C A AG A U C A AG G U G A AC 17 3729

HSV2-UL30-1625 + AAG U CAA AC ACC ACC AC 17 4495

HSV2-UL30-1615 + AGAGCGUACUGAAGCAC 17 4485

HSV2-UL30-1759 + G G CAA A AG UUGUCGCAC 17 4629

HSV2-UL30-971 - UCGGUGUACGGGUUCAC 17 3841

HSV2-UL30-1719 + CAGGUCGUAGAGCAGAC 17 4589

HSV2-UL30-926 - GAGGUCGCGCGCGAGAC 17 3796

HSV2-UL30-1696 + UCAAACUCCAGGACGAC 17 4566

HSV2-UL30-1724 + UCCGGGCGUUCCGCGAC 17 4594

HSV2-UL30-960 - GCAUCCUGCUGCGCGAC 17 3830

HSV2-UL30-847 - ACAAGGUCCCGCUCGAC 17 3717

HSV2-UL30-843 - ACAUCAUCAACUUCGAC 17 3713 HSV2-UL30-2874 + GGGACAUCAGCUUCGAC 17 11228

HSV2-UL30-1032 - UCCCGUCCAUCAAGGAC 17 3902

HSV2-UL30-918 - AGGACGACGACGAGGAC 17 3788

HSV2-UL30-922 - AGGACGGGGACGAGGAC 17 3792

HSV2-UL30-1570 + GCGCAUGGAGUACGGAC 17 4440

HSV2-UL30-949 - CGCACCUGGAGGCGGAC 17 3819

HSV2-UL30-910 - GGGAGCGGCCGGGGGAC 17 3780

HSV2-UL30-1512 + UCCGGUCCUUGAUGGAC 17 4382

HSV2-UL30-1718 + CGAGGGCGGUGGUGGAC 17 4588

HSV2-UL30-841 - GGCCCCGAGUUCGUGAC 17 3711

HSV2-UL30-987 - CCAUGCGCAUCAUCUAC 17 3857

HSV2-UL30-1762 + CGAAGACGCGGUAGUAC 17 4632

HSV2-UL30-1508 + GGGCCACGAUCACGUAC 17 4378

HSV2-UL30-849 - UCCCGCUCGACGGGUAC 17 3719

HSV2-UL30-1622 + GGAUGAUGCUGGGGUAC 17 4492

HSV2-UL30-970 - UGUGCAACUCGGUGUAC 17 3840

HSV2-UL30-1627 + GGGGUCGACGUGAAACC 17 4497

HSV2-UL30-1753 + GU UGUCCAGGAUAAACC 17 4623

HSV2-UL30-815 - CU GCACGG CGG ACAACC 17 3685

HSV2-UL30-802 - GUU UAUCCUGGACAACC 17 3672

HSV2-UL30-800 - GCGUCGACGCCACCACC 17 3670

HSV2-UL30-1563 + UGUCGCCCAUGGCCACC 17 4433

HSV2-UL30-876 - CAAGUU UCUGCCGCACC 17 3746

HSV2-UL30-946 - CGAGGCCGUCGCGCACC 17 3816

HSV2-UL30-972 - CGGUGUACGGGUUCACC 17 3842

HSV2-UL30-927 - AGGUCGCGCGCGAGACC 17 3797

HSV2-UL30-1023 - UCGGCGCAUCACCGACC 17 3893

HSV2-UL30-1548 + A A AAC AG C AG G U CG ACC 17 4418

HSV2-UL30-1633 + AGGUGGGGUCGAGGACC 17 4503

HSV2-UL30-1571 + CGCAUGGAGUACGGACC 17 4441

HSV2-UL30-950 - GCACCUGGAGGCGGACC 17 3820

HSV2-UL30-842 - GCCCCGAG UUCGUGACC 17 3712

HSV2-UL30-951 - GGCGGACCGGGACUACC 17 3821

HSV2-UL30-1460 + CCGGGCGAUGGCGUACC 17 4330

HSV2-UL30-933 - CCGGCACGUUGGGUACC 17 3803

HSV2-UL30-1688 + GUUGAUGAUGUUGUACC 17 4558

HSV2-UL30-1749 + CCGAAGGUGACAAACCC 17 4619

HSV2-UL30-803 - UUUAUCCUGGACAACCC 17 3673

HSV2-UL30-1681 + AC ACG CG G A AC AC ACCC 17 4551

HSV2-UL30-886 - CAUCCUGCCGGACACCC 17 3756

HSV2-UL30-1586 + AAGACCGUGCUGCACCC 17 4456

HSV2-UL30-1634 + GGUGGGGUCGAGGACCC 17 4504

HSV2-UL30-1461 + CGGGCGAUGGCGUACCC 17 4331

HSV2-UL30-1750 + CGAAGGUGACAAACCCC 17 4620

HSV2-UL30-1068 - CGAGACGUGGCACCCCC 17 3938

HSV2-UL30-1046 - GUCGCAUGCCGACCCCC 17 3916 HSV2-UL30-1047 - UCGCAUGCCGACCCCCC 17 3917

HSV2-UL30-1726 + CAGCUCGUCCUCCCCCC 17 4596

HSV2-UL30-1045 - CCCGGCCAAGCGCCCCC 17 3915

HSV2-UL30-1635 + CGAGGACCCGGGCCCCC 17 4505

HSV2-UL30-1458 + GUUGAGCGGAACGCCCC 17 4328

HSV2-UL30-1770 + CCGCGGAACGACGCCCC 17 4640

HSV2-UL30-1646 + GGCCCCGAAACCGCCCC 17 4516

HSV2-UL30-1039 - CUAGACGCCGCCGCCCC 17 3909

HSV2-UL30-1044 - CCCCGGCCAAGCGCCCC 17 3914

HSV2-UL30-985 - GCCGGCAUGCGCGCCCC 17 3855

HSV2-UL30-1701 + GGGGGCCGGCAGGCCCC 17 4571

HSV2-UL30-1637 + CCCAACGUGCCGGCCCC 17 4507

HSV2-UL30-1638 + UCCCCGUUCCCGUCCCC 17 4508

HSV2-UL30-1494 + GGGGCGGGCUCGUCCCC 17 4364

HSV2-UL30-1457 + UGUUGAGCGGAACGCCC 17 4327

HSV2-UL30-1056 - CGGGUACGCCAUCGCCC 17 3926

HSV2-UL30-1550 + CAGGUCGACCAGGGCCC 17 4420

HSV2-UL30-1010 - CCGCACCUCCAGGGCCC 17 3880

HSV2-UL30-938 - UGGGUACCAGGGGGCCC 17 3808

HSV2-UL30-1054 - AGCUGGCGGAGGAUCCC 17 3924

HSV2-UL30-1043 - AGCGGCCCUGCCCUCCC 17 3913

HSV2-UL30-1468 + GCU UGGACGCGCCUCCC 17 4338

HSV2-UL30-1475 + AUGCGACGGCGUCUCCC 17 4345

HSV2-UL30-807 - UGGUACCGCCUCAAGCC 17 3677

HSV2-UL30-832 - UCCGGUCGCGGAACGCC 17 3702

HSV2-UL30-766 - ACGCCAUCACGCCCGCC 17 3636

HSV2-UL30-1659 + UGAUGUUGAUGCCCGCC 17 4529

HSV2-UL30-1773 + CGCGCAGGGCCGCCGCC 17 4643

HSV2-UL30-1027 - GUACACCAACAAGCGCC 17 3897

HSV2-UL30-895 - GGAGGCGCCCAAGCGCC 17 3765

HSV2-UL30-777 - CGAUCUCUGCGAGCGCC 17 3647

HSV2-UL30-877 - U UCCGCCGUCGCGCGCC 17 3747

HSV2-UL30-1055 - CCGGGUACGCCAUCGCC 17 3925

HSV2-UL30-781 - GCCCUGCGCGAGUCGCC 17 3651

HSV2-UL30-823 - AUAUCGAAUGCAAGGCC 17 3693

HSV2-UL30-852 - CGGGCGCAUGAACGGCC 17 3722

HSV2-UL30-1642 + CCCCCCGAGGCACGGCC 17 4512

HSV2-UL30-1732 + UGGCCCCCUCGACGGCC 17 4602

HSV2-UL30-906 - GGGGAAGGGGAGCGGCC 17 3776

HSV2-UL30-1745 + GGCGCGUUCCCGCGGCC 17 4615

HSV2-UL30-994 - CCUCACGGCCGCGGGCC 17 3864

HSV2-UL30-1666 + CCCGCGCUGCGCGGGCC 17 4536

HSV2-UL30-937 - U UGGGUACCAGGGGGCC 17 3807

HSV2-UL30-1519 + ACACCGUCAGGUGGGCC 17 4389

HSV2-UL30-1618 + AGCACAGGU UGUGGGCC 17 4488

HSV2-UL30-1480 + CCCGGGGGCGCU UGGCC 17 4350 HSV2-UL30-1026 - CCCGGAGAGGGACAUCC 17 3896

HSV2-UL30-943 - G U ACCCCAG C AU CAU CC 17 3813

HSV2-UL30-1053 - GAGCUGGCGGAGGAUCC 17 3923

HSV2-UL30-801 - CACCACCCGGU UUAUCC 17 3671

HSV2-UL30-1694 + AU UCGCUGUCAAACUCC 17 4564

HSV2-UL30-940 - UCCUCGACCCCACCUCC 17 3810

HSV2-UL30-1008 - UUAUCAACCGCACCUCC 17 3878

HSV2-UL30-867 - CCGCCUACUACGCCUCC 17 3737

HSV2-UL30-1609 + AGUCCCGGUCCGCCUCC 17 4479

HSV2-UL30-1467 + GGCU UGGACGCGCCUCC 17 4337

HSV2-UL30-834 - UCAGCGAUCUCGCCUCC 17 3704

HSV2-UL30-1662 + CGACGGCGGAAAGCUCC 17 4532

HSV2-UL30-1607 + CCCCCACCUCGAUCUCC 17 4477

HSV2-UL30-1528 + CCUGGAUGUCCCUCUCC 17 4398

HSV2-UL30-1474 + CAUGCGACGGCGUCUCC 17 4344

HSV2-UL30-1526 + CGGUGAGGACAAAGUCC 17 4396

HSV2-UL30-1608 + CGAUCUCCAGGUAGUCC 17 4478

HSV2-UL30-1444 + GCGCGGCCACGUCGUCC 17 4314

HSV2-UL30-838 - GCCGGCCCCCGUCGUCC 17 3708

HSV2-UL30-1786 + AAGCGUGAUGACGGUCC 17 4656

HSV2-UL30-1751 + CA AACCCCG GGUUGUCC 17 4621

HSV2-UL30-1723 + GGAUGACGAGGUCUUCC 17 4593

HSV2-UL30-792 - GCGUCUUCGUGCGAAGC 17 3662

HSV2-UL30-1547 + UAUCGUCGUAAAACAGC 17 4417

HSV2-UL30-1455 + CG CAG G CCGCCCCCAG C 17 4325

HSV2-UL30-1602 + CCAGCCAGUCGCGCAGC 17 4472

HSV2-UL30-1603 + GCAGCAGGAUGCUCAGC 17 4473

HSV2-UL30-829 - CGGGGGGGAGGACGAGC 17 3699

HSV2-UL30-1050 - GCUGCUGGUGUCCGAGC 17 3920

HSV2-UL30-1683 + GCCCGUACCCGUCGAGC 17 4553

HSV2-UL30-2860 + UAAAUCGCUCGUGGAGC 17 11214

HSV2-UL30-1676 + GGCGUUCAGCUUGUAGC 17 4546

HSV2-UL30-1454 + GGCCU UGAACGUCACGC 17 4324

HSV2-UL30-1440 + GUAGCGCCGGCCCCCGC 17 4310

HSV2-UL30-765 - GACGCCAUCACGCCCGC 17 3635

HSV2-UL30-993 - CGCGGCCUCACGGCCGC 17 3863

HSV2-UL30-809 - GCCUCAAGCCCGGCCGC 17 3679

HSV2-UL30-1486 + GGGGAGGGCAGGGCCGC 17 4356

HSV2-UL30-1074 - GCGCGGCUCAGGGCCGC 17 3944

HSV2-UL30-1613 + CGACGGCCUCGGGCCGC 17 4483

HSV2-UL30-869 - CCGGGCCCGCGCAGCGC 17 3739

HSV2-UL30-1439 + UUCCUCCGCCGUAGCGC 17 4309

HSV2-UL30-810 - CGGCCGCGGGAACGCGC 17 3680

HSV2-UL30-977 - CGUACGUGCACGCGCGC 17 3847

HSV2-UL30-1522 + UGU UGGUGUACGCGCGC 17 4392

HSV2-UL30-1030 - CAUGGCCCGCCGCGCGC 17 3900 HSV2-UL30-793 - GCGAAGCGGGCGCGCGC 17 3663

HSV2-UL30-1775 + GCUCGCAGAGAUCGCGC 17 4645

HSV2-UL30-1771 + CCCCCGGCGACUCGCGC 17 4641

HSV2-UL30-1652 + UCUGGCCCGCAAGGCGC 17 4522

HSV2-UL30-1665 + AUCACCCCGCGCUGCGC 17 4535

HSV2-UL30-1757 + UCGUACUUCCUGAUCGC 17 4627

HSV2-UL30-1504 + CGUCUCCUCUACCUCGC 17 4374

HSV2-UL30-780 - GGCCCUGCGCGAGUCGC 17 3650

HSV2-UL30-1710 + GGACUCGGGGAGGUCGC 17 4580

HSV2-UL30-822 - GAUAUCGAAUGCAAGGC 17 3692

HSV2-UL30-1623 + GAUGCUGGGGUACAGGC 17 4493

HSV2-UL30-1584 + GGCGGCCACGUGCAGGC 17 4454

HSV2-UL30-1670 + CCGGAGGCGUAGUAGGC 17 4540

HSV2-UL30-1728 + CACAUGAGCUUG UAGGC 17 4598

HSV2-UL30-851 - ACGGGCGCAUGAACGGC 17 3721

HSV2-UL30-1641 + UCCCCCCGAGGCACGGC 17 4511

HSV2-UL30-1788 + GUGAUGACGGUCCCGGC 17 4658

HSV2-UL30-1729 + UGAGCUUGUAGGCCGGC 17 4599

HSV2-UL30-1080 - GCGGGGUUCGGGCCGGC 17 3950

HSV2-UL30-1700 + GGACGACGGGGGCCGGC 17 4570

HSV2-UL30-1649 + GCCCCUGGGUGUCCGGC 17 4519

HSV2-UL30-905 - GGGGGAAGGGGAGCGGC 17 3775

HSV2-UL30-1744 + CGGCGCGUUCCCGCGGC 17 4614

HSV2-UL30-1515 + CGGGACCUGCGCGCGGC 17 4385

HSV2-UL30-1038 - GGAGACGGUCGCGCGGC 17 3908

HSV2-UL30-984 - GACU UUCCGGAGGCGGC 17 3854

HSV2-UL30-806 - GGUU UGUCACCUUCGGC 17 3676

HSV2-UL30-1484 + GCU UGGCCGGGGAGGGC 17 4354

HSV2-UL30-1536 + CCUGCAGUCCCUCGGGC 17 4406

HSV2-UL30-1078 - GGCCGCGGGGUUCGGGC 17 3948

HSV2-UL30-1699 + UCCAGGACGACGGGGGC 17 4569

HSV2-UL30-930 - CGCGCGAGACCGGGGGC 17 3800

HSV2-UL30-1083 - UUCGGGCCGGCGGGGGC 17 3953

HSV2-UL30-1493 + AGGGCCGCUGGGGGGGC 17 4363

HSV2-UL30-1742 + GGGGGGCGCGGUUGGGC 17 4612

HSV2-UL30-961 - CCUGCUGCGCGACUGGC 17 3831

HSV2-UL30-879 - GCCGUCGCGCGCCUGGC 17 3749

HSV2-UL30-1015 - CCCCGCAGAGGAGUGGC 17 3885

HSV2-UL30-1479 + UCCCGGGGGCGCU UGGC 17 4349

HSV2-UL30-1574 + ACCGGGGGCGCGCAUGC 17 4444

HSV2-UL30-1619 + GUGGGCCUGGAUGAUGC 17 4489

HSV2-UL30-1778 + GCGGGGCACGGCACUGC 17 4648

HSV2-UL30-1018 - CCUGCCCGAGGGACUGC 17 3888

HSV2-UL30-1059 - UUACUUCUCGCACCUGC 17 3929

HSV2-UL30-820 - GGCCAUGUGUGACCUGC 17 3690

HSV2-UL30-837 - CGCCUCCAGGGGCCUGC 17 3707 HSV2-UL30-885 - GAAGGGCU UCAUCCUGC 17 3755

HSV2-UL30-1049 - CAAGCCCCGCAAGCUGC 17 3919

HSV2-UL30-980 - GGAGUUCGAUCAGCUGC 17 3850

HSV2-UL30-873 - UGUGCAGGACUCGCUGC 17 3743

HSV2-UL30-839 - CGAAUUCGAGAUGCUGC 17 3709

HSV2-UL30-1001 - ACAUCGGCGUCAUCUGC 17 3871

HSV2-UL30-1655 + UGAAGACGCGGAUCUGC 17 4525

HSV2-UL30-1540 + GCCAGCCACUCCUCUGC 17 4410

HSV2-UL30-1636 + CCUGGUACCCAACGUGC 17 4506

HSV2-UL30-1583 + CGGUGGCGGCCACGUGC 17 4453

HSV2-UL30-1658 + GCCGUCG UAGAUGGUGC 17 4528

HSV2-UL30-872 - CGGCGAGUAU UGUGUGC 17 3742

HSV2-UL30-882 - UGCCUCCUGCGCCUUGC 17 3752

HSV2-UL30-1464 + GGACACCAGCAGCUUGC 17 4334

HSV2-UL30-1720 + AGAGCAGACAGGAGAUC 17 4590

HSV2-UL30-794 - ACUU UUGCCCCGCGAUC 17 3664

HSV2-UL30-1509 + CGAUCACGUACGGGAUC 17 4379

HSV2-UL30-1007 - CAUCAAGGGCGUGGAUC 17 3877

HSV2-UL30-1556 + CGCACUCGAGCUUGAUC 17 4426

HSV2-UL30-1011 - UACGACGAUACCGUAUC 17 3881

HSV2-UL30-1690 + UACCCGGUCACGAACUC 17 4560

HSV2-UL30-1707 + UCGCUGAGGUGGGACUC 17 4577

HSV2-UL30-939 - GUCCUCGACCCCACCUC 17 3809

HSV2-UL30-1535 + AACGCCUGCAGUCCCUC 17 4405

HSV2-UL30-866 - CCCGCCUACUACGCCUC 17 3736

HSV2-UL30-1575 + CGCAUGCCGGCCGCCUC 17 4445

HSV2-UL30-1612 + AGGUGCGCGACGGCCUC 17 4482

HSV2-UL30-897 - GCGCCCGGCCGUGCCUC 17 3767

HSV2-UL30-1593 + AGGAGGACGGCCUCCUC 17 4463

HSV2-UL30-1545 + UAACGCGGCGGCCGCUC 17 4415

HSV2-UL30-1071 - ACGUGGCCGCGCGGCUC 17 3941

HSV2-UL30-1679 + GCUUCUGAAAGUGGCUC 17 4549

HSV2-UL30-1596 + CCUCCUCGGGGGUGCUC 17 4466

HSV2-UL30-1527 + UCCUGGAUGUCCCUCUC 17 4397

HSV2-UL30-1449 + GGGGGGUGCCACGUCUC 17 4319

HSV2-UL30-1516 + GCU UGUAAUACACCGUC 17 4386

HSV2-UL30-1735 + AGGUUCCGAACGCCGUC 17 4605

HSV2-UL30-1443 + CGCGCGGCCACGUCGUC 17 4313

HSV2-UL30-1765 + CGCGGUAGUACAGGGUC 17 4635

HSV2-UL30-1505 + CCUCUACCUCGCGGGUC 17 4375

HSV2-UL30-875 - AGGACUCGCUGCUGGUC 17 3745

HSV2-UL30-1685 + UCUCCGUCAGCU UGGUC 17 4555

HSV2-UL30-1648 + AACCGCCCCUGGGUGUC 17 4518

HSV2-UL30-1650 + GCAGGAUGAAGCCCUUC 17 4520

HSV2-UL30-1782 + GCGACGCGAUGGCCUUC 17 4652

HSV2-UL30-767 - GACCGUCAUCACGCU UC 17 3637 HSV2-UL30-1722 + UGGAUGACGAGGUCU UC 17 4592

HSV2-UL30-1020 - AGGGACUGCAGGCGUUC 17 3890

HSV2-UL30-1077 - UCAGGGCCGCGGGGU UC 17 3947

HSV2-UL30-981 - GCUGCUGGCCGACU U UC 17 3851

HSV2-UL30-830 - GGACGAGCUGGCCUUUC 17 3700

HSV2-UL30-891 - CACCCAGGGGCGGUUUC 17 3761

HSV2-UL30-1678 + UGCUGCGCUUCUGAAAG 17 4548

HSV2-UL30-1066 - CCGAGAGUCUGUUAAAG 17 3936

HSV2-UL30-791 - CGCGUCU UCGUGCGAAG 17 3661

HSV2-UL30-1767 + CCACCUCCGCCUCGAAG 17 4637

HSV2-UL30-903 - CGUGCCUCGGGGGGAAG 17 3773

HSV2-UL30-935 - GGCACGUUGGGUACCAG 17 3805

HSV2-UL30-888 - UCCUGCCGGACACCCAG 17 3758

HSV2-UL30-1042 - CGAGCCCGCCCCCCCAG 17 3912

HSV2-UL30-1041 - AG ACG CCG CCG CCCC AG 17 3911

HSV2-UL30-836 - AGCGAUCUCGCCUCCAG 17 3706

HSV2-UL30-1013 - AGCCGAGCGCCCCGCAG 17 3883

HSV2-UL30-1538 + UGCAGUCCCUCGGGCAG 17 4408

HSV2-UL30-797 - CGAUCAGGAAGUACGAG 17 3667

HSV2-UL30-1600 + UCUGGGGGAUCCGCGAG 17 4470

HSV2-UL30-1682 + CGCCCG UACCCGUCGAG 17 4552

HSV2-UL30-818 - ACAACCUGGCCGUCGAG 17 3688

HSV2-UL30-1014 - AGCGCCCCGCAGAGGAG 17 3884

HSV2-UL30-1024 - GCAUCACCGACCCGGAG 17 3894

HSV2-UL30-1012 - CGAUACCGUAUCCGGAG 17 3882

HSV2-UL30-904 - CUCGGGGGGAAGGGGAG 17 3774

HSV2-UL30-1456 + UAAUAGUCCGUGU UGAG 17 4326

HSV2-UL30-1035 - CCAGACCCGCGAGGUAG 17 3905

HSV2-UL30-913 - GCCGGGGGACGGGAACG 17 3783

HSV2-UL30-1543 + GGGGCGCUCGGCUAACG 17 4413

HSV2-UL30-1680 + GGCCGAUGUCCCACACG 17 4550

HSV2-UL30-1626 + AG U C A A AC ACC ACC ACG 17 4496

HSV2-UL30-771 - UUCACGUCUACGGCACG 17 3641

HSV2-UL30-975 - UCUGCCCUGCCUGCACG 17 3845

HSV2-UL30-1761 + CGCUUCGCACGAAGACG 17 4631

HSV2-UL30-1654 + GGAGGCACGUGAAGACG 17 4524

HSV2-UL30-1067 - GGU U U AU UCCCG AG ACG 17 3937

HSV2-UL30-916 - GGAUAAGGACGACGACG 17 3786

HSV2-UL30-1697 + CAAACUCCAGGACGACG 17 4567

HSV2-UL30-1069 - GCACCCCCCGGACGACG 17 3939

HSV2-UL30-2875 + GGACAUCAGCUUCGACG 17 11229

HSV2-UL30-919 - GGACGACGACGAGGACG 17 3789

HSV2-UL30-923 - GGACGGGGACGAGGACG 17 3793

HSV2-UL30-914 - GGACGGGAACGGGGACG 17 3784

HSV2-UL30-920 - CGACGAGGACGGGGACG 17 3790

HSV2-UL30-1721 + AGGAGAUCUGGAUGACG 17 4591 HSV2-UL30-988 - CAUGCGCAUCAUCUACG 17 3858

HSV2-UL30-1532 + GCCGAUGGGCGUCUACG 17 4402

HSV2-UL30-795 - CGCGAUCAGGAAGUACG 17 3665

HSV2-UL30-1604 + GCAGGCUCUCGCGUACG 17 4474

HSV2-UL30-973 - GGUGUACGGGUUCACCG 17 3843

HSV2-UL30-928 - GGUCGCGCGCGAGACCG 17 3798

HSV2-UL30-1572 + GCAUGGAGUACGGACCG 17 4442

HSV2-UL30-804 - UUAUCCUGGACAACCCG 17 3674

HSV2-UL30-1441 + CGCCGGCCCGAACCCCG 17 4311

HSV2-UL30-941 - GUUUCACGUCGACCCCG 17 3811

HSV2-UL30-963 - CCCCCAGAGCACCCCCG 17 3833

HSV2-UL30-1639 + CGCUCCCCU UCCCCCCG 17 4509

HSV2-UL30-1057 - GGGUACGCCAUCGCCCG 17 3927

HSV2-UL30-1564 + CAUGGCCACCAGGCCCG 17 4434

HSV2-UL30-945 - GCUCUCCCUGCGGCCCG 17 3815

HSV2-UL30-1016 - GGCGCGACCCCUGCCCG 17 3886

HSV2-UL30-1469 + CU UGGACGCGCCUCCCG 17 4339

HSV2-UL30-1476 + UGCGACGGCGUCUCCCG 17 4346

HSV2-UL30-1743 + GGGCCGGCGCGU UCCCG 17 4613

HSV2-UL30-782 - CCCUGCGCGAGUCGCCG 17 3652

HSV2-UL30-863 - GCUGAACGCCGUCGCCG 17 3733

HSV2-UL30-824 - UAUCGAAUGCAAGGCCG 17 3694

HSV2-UL30-1566 + CCGCGGCCGUGAGGCCG 17 4436

HSV2-UL30-853 - GGGCGCAUGAACGGCCG 17 3723

HSV2-UL30-992 - CCGCGGCCUCACGGCCG 17 3862

HSV2-UL30-808 - CGCCUCAAGCCCGGCCG 17 3678

HSV2-UL30-907 - GGGAAGGGGAGCGGCCG 17 3777

HSV2-UL30-1073 - CGCGCGGCUCAGGGCCG 17 3943

HSV2-UL30-1481 + CCGGGGGCGCUUGGCCG 17 4351

HSV2-UL30-1769 + GGUCCGCGGAGAUGCCG 17 4639

HSV2-UL30-990 - AUUU UCGUU UUGUGCCG 17 3860

HSV2-UL30-785 - GUUCCGCGGCAUCUCCG 17 3655

HSV2-UL30-1445 + CGCGGCCACGUCGUCCG 17 4315

HSV2-UL30-1768 + CGCCUCGAAGUGGUCCG 17 4638

HSV2-UL30-784 - CCGGGGGCGUCGU UCCG 17 3654

HSV2-UL30-868 - UCCGGGCCCGCGCAGCG 17 3738

HSV2-UL30-1034 - CGUGGCCCAGACCCGCG 17 3904

HSV2-UL30-1442 + CGCGGCCCUGAGCCGCG 17 4312

HSV2-UL30-1075 - CGCGGCUCAGGGCCGCG 17 3945

HSV2-UL30-1070 - CGGACGACGUGGCCGCG 17 3940

HSV2-UL30-870 - CGGGCCCGCGCAGCGCG 17 3740

HSV2-UL30-924 - GGACGGGGACGAGCGCG 17 3794

HSV2-UL30-1521 + UUGU UGGUGUACGCGCG 17 4391

HSV2-UL30-1776 + CUCGCAGAGAUCGCGCG 17 4646

HSV2-UL30-1513 + UGGACGGGACCUGCGCG 17 4383

HSV2-UL30-1774 + CGCUCGCAGAGAUCGCG 17 4644 HSV2-UL30-1758 + CGUACUUCCUGAUCGCG 17 4628

HSV2-UL30-1577 + CGUACGCGCGCGUCGCG 17 4447

HSV2-UL30-1037 - UAGAGGAGACGGUCGCG 17 3907

HSV2-UL30-1671 + CGGAGGCGUAGUAGGCG 17 4541

HSV2-UL30-1081 - CGGGGUUCGGGCCGGCG 17 3951

HSV2-UL30-1006 - GAUGCUCAUCAAGGGCG 17 3876

HSV2-UL30-1739 + AACGCCGUCGGGGGGCG 17 4609

HSV2-UL30-1664 + GAUCACCCCGCGCUGCG 17 4534

HSV2-UL30-1002 - CAUCGGCGUCAUCUGCG 17 3872

HSV2-UL30-1541 + CCAGCCACUCCUCUGCG 17 4411

HSV2-UL30-1465 + GACACCAGCAGCUUGCG 17 4335

HSV2-UL30-952 - GGACUACCUGGAGAUCG 17 3822

HSV2-UL30-1756 + CUCGUACU UCCUGAUCG 17 4626

HSV2-UL30-1033 - GAUCCCGUACGUGAUCG 17 3903

HSV2-UL30-1557 + GCACUCGAGCUUGAUCG 17 4427

HSV2-UL30-1691 + ACCCGGUCACGAACUCG 17 4561

HSV2-UL30-1708 + CGCUGAGGUGGGACUCG 17 4578

HSV2-UL30-1503 + CCGUCUCCUCUACCUCG 17 4373

HSV2-UL30-898 - CGCCCGGCCGUGCCUCG 17 3768

HSV2-UL30-1594 + GGAGGACGGCCUCCUCG 17 4464

HSV2-UL30-1499 + CGGCGGCGUCUAGCUCG 17 4369

HSV2-UL30-962 - GAAAGCAGAUCCGCUCG 17 3832

HSV2-UL30-1790 + CGUCCAUAAAUCGCUCG 17 4660

HSV2-UL30-1733 + AAACUCGACGUCGCUCG 17 4603

HSV2-UL30-1713 + ACAGGAGGAUG UGCUCG 17 4583

HSV2-UL30-1578 + UCGCGAGGAGCAUCUCG 17 4448

HSV2-UL30-1736 + GGUUCCGAACGCCGUCG 17 4606

HSV2-UL30-816 - GGACAACCUGGCCGUCG 17 3686

HSV2-UL30-831 - GCUGGCCUU UCCGGUCG 17 3701

HSV2-UL30-1632 + ACCCGGAGGUGGGGUCG 17 4502

HSV2-UL30-1672 + AGUAGGCGGGGAUGUCG 17 4542

HSV2-UL30-1645 + UGGGCGCCUCCU UGUCG 17 4515

HSV2-UL30-1589 + CGGCCUGU UGCUUGUCG 17 4459

HSV2-UL30-786 - CUCCGCGGACCACUUCG 17 3656

HSV2-UL30-1783 + CGACGCGAUGGCCUUCG 17 4653

HSV2-UL30-1021 - GGGACUGCAGGCGUUCG 17 3891

HSV2-UL30-892 - ACCCAGGGGCGGUUUCG 17 3762

HSV2-UL30-773 - CU UUUACAUGAACAAGG 17 3643

HSV2-UL30-894 - UCGGGGCCUCGACAAGG 17 3764

HSV2-UL30-967 - ACAGGCCGCCAUCAAGG 17 3837

HSV2-UL30-1712 + AUCCGAGCGAAAACAGG 17 4582

HSV2-UL30-936 - GCACGUUGGGUACCAGG 17 3806

HSV2-UL30-1653 + GGCCCGCAAGGCGCAGG 17 4523

HSV2-UL30-1517 + U G U A AU ACACCG U CAG G 17 4387

HSV2-UL30-798 - GAUCAGGAAGUACGAGG 17 3668

HSV2-UL30-964 - CCAGAGCACCCCCGAGG 17 3834 HSV2-UL30-925 - CGGGGACGAGCGCGAGG 17 3795

HSV2-UL30-953 - CUACCUGGAGAUCGAGG 17 3823

HSV2-UL30-819 - CAACCUGGCCGUCGAGG 17 3689

HSV2-UL30-1590 + CCUGUUGCU UGUCGAGG 17 4460

HSV2-UL30-787 - CGCGG ACCACU UCG AGG 17 3657

HSV2-UL30-1629 + GACGUGAAACCCGGAGG 17 4499

HSV2-UL30-983 - GGCCGACUU UCCGGAGG 17 3853

HSV2-UL30-775 - CAUGAACAAGGCGGAGG 17 3645

HSV2-UL30-789 - CCACU UCGAGGCGG AGG 17 3659

HSV2-UL30-948 - CGUCGCGCACCUGGAGG 17 3818

HSV2-UL30-1704 + AGGCGAGAUCGCUGAGG 17 4574

HSV2-UL30-1747 + CGCGGCCGGGCUUGAGG 17 4617

HSV2-UL30-1669 + CCCGGAGGCGUAGUAGG 17 4539

HSV2-UL30-814 - CGAGU UUAACUGCACGG 17 3684

HSV2-UL30-1581 + GCCGAUGGUCGUCACGG 17 4451

HSV2-UL30-1698 + AAACUCCAGGACGACGG 17 4568

HSV2-UL30-1661 + CGCCAGGCGCGCGACGG 17 4531

HSV2-UL30-2876 + GACAUCAGCU UCGACGG 17 11230

HSV2-UL30-1085 - GGGGGCCGGCGCUACGG 17 3955

HSV2-UL30-929 - GUCGCGCGCGAGACCGG 17 3799

HSV2-UL30-1573 + CAUGGAGUACGGACCGG 17 4443

HSV2-UL30-1628 + GUCGACGUGAAACCCGG 17 4498

HSV2-UL30-1667 + GCGCUGCGCGGGCCCGG 17 4537

HSV2-UL30-1470 + UUGGACGCGCCUCCCGG 17 4340

HSV2-UL30-1477 + GCGACGGCGUCUCCCGG 17 4347

HSV2-UL30-1787 + CGUGAUGACGGUCCCGG 17 4657

HSV2-UL30-783 - CCUGCGCGAGUCGCCGG 17 3653

HSV2-UL30-825 - AUCGAAUGCAAGGCCGG 17 3695

HSV2-UL30-908 - GGAAGGGGAGCGGCCGG 17 3778

HSV2-UL30-1079 - CGCGGGGUUCGGGCCGG 17 3949

HSV2-UL30-1446 + GCGGCCACGUCGUCCGG 17 4316

HSV2-UL30-982 - GCUGGCCGACUUUCCGG 17 3852

HSV2-UL30-1544 + GCGCUCGGCUAACGCGG 17 4414

HSV2-UL30-1514 + ACGGGACCUGCGCGCGG 17 4384

HSV2-UL30-1500 + CGGCGUCUAGCUCGCGG 17 4370

HSV2-UL30-774 - U U AC A U G A AC A AG G CG G 17 3644

HSV2-UL30-788 - GG ACCACU UCGAGGCGG 17 3658

HSV2-UL30-1086 - GGCCGGCGCUACGGCGG 17 3956

HSV2-UL30-1082 - GGGGUUCGGGCCGGCGG 17 3952

HSV2-UL30-1716 + GAUGUGCUCGAGGGCGG 17 4586

HSV2-UL30-1498 + CUCGUCCCCUGGGGCGG 17 4368

HSV2-UL30-779 - CUGCGAGCGCCUGGCGG 17 3649

HSV2-UL30-1052 - GGUGUCCGAGCUGGCGG 17 3922

HSV2-UL30-1003 - AUCGGCGUCAUCUGCGG 17 3873

HSV2-UL30-1558 + CACUCGAGCUUGAUCGG 17 4428

HSV2-UL30-899 - GCCCGGCCGUGCCUCGG 17 3769 HSV2-UL30-1595 + GAGGACGGCCUCCUCGG 17 4465

HSV2-UL30-1737 + GUUCCGAACGCCGUCGG 17 4607

HSV2-UL30-1784 + GACGCGAUGGCCU UCGG 17 4654

HSV2-UL30-1524 + GUGUCUGCUCAGU UCGG 17 4394

HSV2-UL30-799 - AUCAGGAAGUACGAGGG 17 3669

HSV2-UL30-1715 + GAGGAUGUGCUCGAGGG 17 4585

HSV2-UL30-1502 + GUCUAGCUCGCGGAGGG 17 4372

HSV2-UL30-2877 + ACAUCAGCUUCGACGGG 17 11231

HSV2-UL30-1754 + GUCCAGGAUAAACCGGG 17 4624

HSV2-UL30-1048 - CAUGCCGACCCCCCGGG 17 3918

HSV2-UL30-1471 + UGGACGCGCCUCCCGGG 17 4341

HSV2-UL30-826 - UCGAAUGCAAGGCCGGG 17 3696

HSV2-UL30-1447 + CGGCCACGUCGUCCGGG 17 4317

HSV2-UL30-1559 + ACUCGAGCUUGAUCGGG 17 4429

HSV2-UL30-900 - CCCGGCCGUGCCUCGGG 17 3770

HSV2-UL30-1738 + UUCCGAACGCCGUCGGG 17 4608

HSV2-UL30-889 - UGCCGGACACCCAGGGG 17 3759

HSV2-UL30-827 - CGAAUGCAAGGCCGGGG 17 3697

HSV2-UL30-1482 + GGGGCGCUUGGCCGGGG 17 4352

HSV2-UL30-1709 + UGAGGUGGGACUCGGGG 17 4579

HSV2-UL30-1560 + CGAGCUUGAUCGGGGGG 17 4430

HSV2-UL30-828 - AUGCAAGGCCGGGGGGG 17 3698

HSV2-UL30-1492 + CAGGGCCGCUGGGGGGG 17 4362

HSV2-UL30-1063 - CUCGCACCUGCUGGGGG 17 3933

HSV2-UL30-1497 + GGGCUCGUCCCCUGGGG 17 4367

HSV2-UL30-1491 + GGGCAGGGCCGCUGGGG 17 4361

HSV2-UL30-2868 + UCGCUCGUGGAGCUGGG 17 11222

HSV2-UL30-1490 + AGGGCAGGGCCGCUGGG 17 4360

HSV2-UL30-979 - CGUGCACGCGCGCUGGG 17 3849

HSV2-UL30-957 - CUGGAGAUCGAGGUGGG 17 3827

HSV2-UL30-1588 + GCACACCACCU UG AUGG 17 4458

HSV2-UL30-947 - GGCCGUCGCGCACCUGG 17 3817

HSV2-UL30-1702 + GGCCGGCAGGCCCCUGG 17 4572

HSV2-UL30-1551 + GUCGACCAGGGCCCUGG 17 4421

HSV2-UL30-778 - UCUCUGCGAGCGCCUGG 17 3648

HSV2-UL30-878 - CGCCGUCGCGCGCCUGG 17 3748

HSV2-UL30-995 - CACGGCCGCGGGCCUGG 17 3865

HSV2-UL30-1051 - GCUGGUGUCCGAGCUGG 17 3921

HSV2-UL30-1489 + GAGGGCAGGGCCGCUGG 17 4359

HSV2-UL30-1062 - CUUCUCGCACCUGCUGG 17 3932

HSV2-UL30-1599 + CCUCGGGGGUGCUCUGG 17 4469

HSV2-UL30-942 - UCACGUCGACCCCGUGG 17 3812

HSV2-UL30-956 - CCUGGAGAUCGAGGUGG 17 3826

HSV2-UL30-790 - CU UCGAGGCGGAGGUGG 17 3660

HSV2-UL30-1582 + GAUGGUCGUCACGGUGG 17 4452

HSV2-UL30-1717 + GUGCUCGAGGGCGGUGG 17 4587 HSV2-UL30-1755 + CAGGAUAAACCGGGUGG 17 4625

HSV2-UL30-1561 + ACAGCGCGCGCGAGAUG 17 4431

HSV2-UL30-1496 + GGCGGGCUCGUCCCCUG 17 4366

HSV2-UL30-944 - UCAGUACGCUCUCCCUG 17 3814

HSV2-UL30-1488 + GGAGGGCAGGGCCGCUG 17 4358

HSV2-UL30-1703 + UGGAGGCGAGAUCGCUG 17 4573

HSV2-UL30-1621 + GGGCCUGGAUGAUGCUG 17 4491

HSV2-UL30-1061 - ACUUCUCGCACCUGCUG 17 3931

HSV2-UL30-1000 - UACAUCGGCGUCAUCUG 17 3870

HSV2-UL30-1539 + CGCCAGCCACUCCUCUG 17 4409

HSV2-UL30-1598 + UCCUCGGGGGUGCUCUG 17 4468

HSV2-UL30-1565 + CCAGGCCCGCGGCCGUG 17 4435

HSV2-UL30-854 - GGGGUGUGUUCCGCGUG 17 3724

HSV2-UL30-1663 + GCGGAAAGCUCCAGGUG 17 4533

HSV2-UL30-955 - ACCUGGAGAUCGAGGUG 17 3825

HSV2-UL30-1631 + CGUGAAACCCGGAGGUG 17 4501

HSV2-UL30-1552 + CCAGGGCCCUGGAGGUG 17 4422

HSV2-UL30-1525 + UGCUCAGU UCGGCGGUG 17 4395

HSV2-UL30-1657 + GGCCGUCGUAGAUGGUG 17 4527

HSV2-UL30-881 - GUGCCUCCUGCGCCUUG 17 3751

HSV2-UL30-1463 + CGGACACCAGCAGCUUG 17 4333

HSV2-UL30-1746 + UCCCGCGGCCGGGCU UG 17 4616

HSV2-UL30-1616 + UACUGAAGCACAGGUUG 17 4486

HSV2-UL30-856 - UUCCGCGUGUGGGACAU 17 3726

HSV2-UL30-999 - GCCAAGAAAAAGUACAU 17 3869

HSV2-UL30-976 - GCCACCGUGACGACCAU 17 3846

HSV2-UL30-1022 - UCCUCGUAGACGCCCAU 17 3892

HSV2-UL30-997 - GCGGGCCUGGUGGCCAU 17 3867

HSV2-UL30-1531 + GUCGGUGAUGCGCCGAU 17 4401

HSV2-UL30-776 - ACAAGGCGGAGGUGGAU 17 3646

HSV2-UL30-871 - GCGCAGCGCGGGGUGAU 17 3741

HSV2-UL30-1555 + UCGCACUCGAGCU UGAU 17 4425

HSV2-UL30-968 - CAAGGUGGUGUGCAACU 17 3838

HSV2-UL30-1689 + GUACCCGGUCACGAACU 17 4559

HSV2-UL30-1706 + AUCGCUGAGGUGGGACU 17 4576

HSV2-UL30-1647 + GCCCCGAAACCGCCCCU 17 4517

HSV2-UL30-1495 + GGGCGGGCUCGUCCCCU 17 4365

HSV2-UL30-1534 + GAACGCCUGCAGUCCCU 17 4404

HSV2-UL30-1674 + GUCCUUCAAGACGGCCU 17 4544

HSV2-UL30-1611 + CAGGUGCGCGACGGCCU 17 4481

HSV2-UL30-896 - AGCGCCCGGCCGUGCCU 17 3766

HSV2-UL30-1592 + GAGGAGGACGGCCUCCU 17 4462

HSV2-UL30-1462 + GGGAUCCUCCGCCAGCU 17 4332

HSV2-UL30-1554 + GGCGAUGAGCAGCAGCU 17 4424

HSV2-UL30-1684 + GUAGAUCUCCGUCAGCU 17 4554

HSV2-UL30-2869 + AAAUCGCUCGUGGAGCU 17 11223 HSV2-UL30-1487 + GGGAGGGCAGGGCCGCU 17 4357

HSV2-UL30-978 - GUACGUGCACGCGCGCU 17 3848

HSV2-UL30-1643 + AGGCACGGCCGGGCGCU 17 4513

HSV2-UL30-1542 + CUCCUCUGCGGGGCGCU 17 4412

HSV2-UL30-1478 + CGUCUCCCGGGGGCGCU 17 4348

HSV2-UL30-833 - AUCCUCCUGUUUUCGCU 17 3703

HSV2-UL30-1466 + CAGCAGCUUGCGGGGCU 17 4336

HSV2-UL30-1620 + UGGGCCUGGAUGAUGCU 17 4490

HSV2-UL30-1060 - UACU UCUCGCACCUGCU 17 3930

HSV2-UL30-1451 + CAGACUCUCGGUGAUCU 17 4321

HSV2-UL30-1450 + CCUCUU UAACAGACUCU 17 4320

HSV2-UL30-1597 + CUCCUCGGGGGUGCUCU 17 4467

HSV2-UL30-1448 + CGGGGGGUGCCACGUCU 17 4318

HSV2-UL30-1506 + CUCUACCUCGCGGGUCU 17 4376

HSV2-UL30-768 - ACCGUCAUCACGCUUCU 17 3638

HSV2-UL30-1553 + GCCGAUGUACU UUUUCU 17 4423

HSV2-UL30-1576 + GGCCGCCUCCGGAAAGU 17 4446

HSV2-UL30-1668 + GGGCCCGGAGGCGUAGU 17 4538

HSV2-UL30-1766 + U UCGUAAUAGUACACGU 17 4636

HSV2-UL30-931 - ACCGGGGGCCGGCACGU 17 3801

HSV2-UL30-1605 + CAGGCUCUCGCGUACGU 17 4475

HSV2-UL30-1606 + U CACG AAG A ACAG CCG U 17 4476

HSV2-UL30-1734 + G AG G U U CCG AACG CCG U 17 4604

HSV2-UL30-1760 + AAAG U UG U CGCACAGG U 17 4630

HSV2-UL30-1518 + GUAAUACACCGUCAGGU 17 4388

HSV2-UL30-954 - UACCUGGAGAUCGAGGU 17 3824

HSV2-UL30-1630 + ACGUGAAACCCGGAGGU 17 4500

HSV2-UL30-1705 + GGCGAGAUCGCUGAGGU 17 4575

HSV2-UL30-1740 + CCGUCGGGGGGCGCGGU 17 4610

HSV2-UL30-1764 + ACGCGGUAGUACAGGGU 17 4634

HSV2-UL30-1529 + GAUGUCCCUCUCCGGGU 17 4399

HSV2-UL30-1472 + CGCGCCUCCCGGGGGGU 17 4342

HSV2-UL30-874 - CAGGACUCGCUGCUGG U 17 3744

HSV2-UL30-855 - GGGUGUGUUCCGCGUG U 17 3725

HSV2-UL30-1677 + GGAGAG UUUGACCU UGU 17 4547

HSV2-UL30-1727 + GAAGCACAUGAGCUUGU 17 4597

HSV2-UL30-1520 + GUGGGCCAGGCGCU UGU 17 4390

HSV2-UL30-1617 + ACUGAAGCACAGGUUGU 17 4487

HSV2-UL30-805 - CCGGGG UUUGUCACCUU 17 3675

HSV2-UL30-1781 + GGCGACGCGAUGGCCU U 17 4651

HSV2-UL30-1644 + GGCACGGCCGGGCGCUU 17 4514

HSV2-UL30-1523 + CGGGUGUCUGCUCAGU U 17 4393

HSV2-UL30-932 - CCGGGGGCCGGCACGUU 17 3802

HSV2-UL30-1019 - GAGGGACUGCAGGCGU U 17 3889

HSV2-UL30-812 - CGCCCCCCGACGGCGU U 17 3682

HSV2-UL30-1741 + CGUCGGGGGGCGCGGU U 17 4611 HSV2-UL30-1076 - CUCAGGGCCGCGGGGUU 17 3946

HSV2-UL30-1065 - ACGUUCAAGGCCCUGUU 17 3935

HSV2-UL30-890 - ACACCCAGGGG CGG U U U 17 3760

HSV2-UL30-1215 + G CCG CG G C AC A A A ACG A A A A 20 4085

HSV2-UL30-683 - GGGGGCAAGAUGCUCAUCAA 20 3553

HSV2-UL30-2878 + GGCUCAUGCUAGAGUAUCAA 20 11232

HSV2-UL30-562 - CUGCGCCU UGCGGGCCAGAA 20 3432

HSV2-UL30-1335 + GUCAGCUUGGUCAGGACGAA 20 4205

HSV2-UL30-1373 + CGGGCGUUCCGCGACCGGAA 20 4243

HSV2-UL30-589 - GAGCGGCCGGGGGACGGGAA 20 3459

HSV2-UL30-580 - CGGCCGUGCCUCGGGGGGAA 20 3450

HSV2-UL30-528 - GACGGGUACGGGCGCAUGAA 20 3398

HSV2-UL30-1427 + CCGCGUGCCGUAGACGUGAA 20 4297

HSV2-UL30-536 - CG C AG C A AG A U C A AG G U G A A 20 3406

HSV2-UL30-1101 + CUUGGCGU UAUUUCCAAACA 20 3971

HSV2-UL30-736 - CCGGGGCGUUCCGCUCAACA 20 3606

HSV2-UL30-450 - GCAGUACU UUUACAUGAACA 20 3320

HSV2-UL30-1378 + G U AGG CCGGCAG G U CACACA 20 4248

HSV2-UL30-540 - GUACGGCAUCAUCACCGACA 20 3410

HSV2-UL30-571 - GCGGUUUCGGGGCCUCGACA 20 3441

HSV2-UL30-667 - GCGCAUCAUCUACGGGGACA 20 3537

HSV2-UL30-523 - CAAGCUGACGGAGAUCUACA 20 3393

HSV2-UL30-1411 + CACGAAGACGCGGUAGUACA 20 4281

HSV2-UL30-1272 + GGCAAAG UCAAACACCACCA 20 4142

HSV2-UL30-1197 + GUAAAACAGCAGGUCGACCA 20 4067

HSV2-UL30-612 - GGCCGGCACGUUGGGUACCA 20 3482

HSV2-UL30-565 - UUCAUCCUGCCGGACACCCA 20 3435

HSV2-UL30-718 - AGCUAGACGCCGCCGCCCCA 20 3588

HSV2-UL30-1210 + GCUCGCCAUCUUG UCGCCCA 20 4080

HSV2-UL30-674 - GGCCGCGGGCCUGGUGGCCA 20 3544

HSV2-UL30-687 - GUUUAUCAACCGCACCUCCA 20 3557

HSV2-UL30-513 - CCUCAGCGAUCUCGCCUCCA 20 3383

HSV2-UL30-652 - GGGUUCACCGGGGUGCAGCA 20 3522

HSV2-UL30-1299 + UGAAGCCCUUCUGGCCCGCA 20 4169

HSV2-UL30-1262 + CGCGACGGCCUCGGGCCGCA 20 4132

HSV2-UL30-1420 + CGCCCCCGGCGACUCGCGCA 20 4290

HSV2-UL30-1216 + GUCCCCGUAGAUGAUGCGCA 20 4086

HSV2-UL30-1249 + CGAGCGGAUCUGCUUUCGCA 20 4119

HSV2-UL30-1233 + GUGGCGGCCACGUGCAGGCA 20 4103

HSV2-UL30-1288 + CUCCCCU UCCCCCCGAGGCA 20 4158

HSV2-UL30-1133 + GCGCUUGGCCGGGGAGGGCA 20 4003

HSV2-UL30-1185 + CGCCUGCAGUCCCUCGGGCA 20 4055

HSV2-UL30-1425 + CGCAGAGAUCGCGCGGGGCA 20 4295

HSV2-UL30-499 - GUGCU UCGAUAUCGAAUGCA 20 3369

HSV2-UL30-491 - CGACGUCGAGU UUAACUGCA 20 3361

HSV2-UL30-644 - CAAGCAACAGGCCGCCAUCA 20 3514 HSV2-UL30-709 - CGCGCAGGUCCCGUCCAUCA 20 3579

HSV2-UL30-682 - CGGGGGCAAGAUGCUCAUCA 20 3552

HSV2-UL30-535 - UCAGAAGCGCAGCAAGAUCA 20 3405

HSV2-UL30-669 - CGUUUUGUGCCGCGGCCUCA 20 3539

HSV2-UL30-707 - GACGGUGUAU UACAAGCUCA 20 3577

HSV2-UL30-750 - CGACGUGGCCGCGCGGCUCA 20 3620

HSV2-UL30-1228 + CUCGCGGCCGAUGG UCGUCA 20 4098

HSV2-UL30-742 - GGCGGCCUGCGUGACGU UCA 20 3612

HSV2-UL30-676 - GGUGGCCAUGGGCGACAAGA 20 3546

HSV2-UL30-1321 + CUUCUUCUUGUCCUUCAAGA 20 4191

HSV2-UL30-543 - CGUCUUGAAGGACAAGAAGA 20 3413

HSV2-UL30-561 - CCUGCGCCU UGCGGGCCAGA 20 3431

HSV2-UL30-714 - GACCCGCGAGGUAGAGGAGA 20 3584

HSV2-UL30-703 - GCGCAUCACCGACCCGGAGA 20 3573

HSV2-UL30-1304 + GAUCUGCUGGCCGUCGUAGA 20 4174

HSV2-UL30-1343 + GCUGUCAAACUCCAGGACGA 20 4213

HSV2-UL30-1334 + CGUCAGCUUGGUCAGGACGA 20 4204

HSV2-UL30-1340 + GGGGCCGUACUGCU UGACGA 20 4210

HSV2-UL30-558 - AUCACCCGCACCAUCUACGA 20 3428

HSV2-UL30-474 - CCCGCGAUCAGGAAGUACGA 20 3344

HSV2-UL30-447 - CUUCUGGGUCUGACCCCCGA 20 3317

HSV2-UL30-489 - GGCCCAACCGCGCCCCCCGA 20 3359

HSV2-UL30-695 - CUGGCGCGACCCCUGCCCGA 20 3565

HSV2-UL30-1396 + CUUGAGGCGGUACCAGCCGA 20 4266

HSV2-UL30-1178 + CCGGGUCGGUGAUGCGCCGA 20 4048

HSV2-UL30-1227 + GAGGAGCAUCUCGCGGCCGA 20 4097

HSV2-UL30-636 - AGAUCGAGGUGGGGGGCCGA 20 3506

HSV2-UL30-1428 + AGACGUGAACGGCGACGCGA 20 4298

HSV2-UL30-1308 + GAUGCCCGCCAGGCGCGCGA 20 4178

HSV2-UL30-1258 + GUCCGCCUCCAGGUGCGCGA 20 4128

HSV2-UL30-1323 + CU UCAAG ACGGCCUCGGCG A 20 4193

HSV2-UL30-1107 + GAGCGGAACGCCCCGGGCGA 20 3977

HSV2-UL30-1121 + CCCGGGGGGUCGGCAUGCGA 20 3991

HSV2-UL30-1379 + GUCACACAUGGCCCCCUCGA 20 4249

HSV2-UL30-524 - AUCUACAAGGUCCCGCUCGA 20 3394

HSV2-UL30-1362 + AAACAGGAGGAUGUGCUCGA 20 4232

HSV2-UL30-495 - GCGGACAACCUGGCCGUCGA 20 3365

HSV2-UL30-2879 + AUGCGGGACAUCAGCUUCGA 20 11233

HSV2-UL30-595 - GAUAAGGACGACGACGAGGA 20 3465

HSV2-UL30-599 - GACGAGGACGGGGACGAGGA 20 3469

HSV2-UL30-1181 + CCGAUGGGCG UCUACGAGGA 20 4051

HSV2-UL30-1239 + CUGUUGCU UGUCGAGGAGGA 20 4109

HSV2-UL30-1149 + GGCGGCGUCUAGCUCGCGGA 20 4019

HSV2-UL30-538 - CAAGAUCAAGGUGAACGGGA 20 3408

HSV2-UL30-1131 + CGGGGGCGCU UGGCCGGGGA 20 4001

HSV2-UL30-587 - GAAGGGGAGCGGCCGGGGGA 20 3457 HSV2-UL30-579 - CCGGCCGUGCCUCGGGGGGA 20 3449

HSV2-UL30-1159 + GGGAUCCGGUCCUUGAUGGA 20 4029

HSV2-UL30-1341 + CUGCUUGACGAAGGUCAUGA 20 4211

HSV2-UL30-1433 + CAGACCCAGAAGCGUGAUGA 20 4303

HSV2-UL30-706 - CAAGCGCCUGGCCCACCUGA 20 3576

HSV2-UL30-522 - CUUCGUCCUGACCAAGCUGA 20 3392

HSV2-UL30-1437 + GACGGUCCCGGCGGGCGUGA 20 4307

HSV2-UL30-637 - CCGACGGCUGU UCUUCGUGA 20 3507

HSV2-UL30-1235 + CGAGUUGCACACCACCUUGA 20 4105

HSV2-UL30-1158 + GUACGGGAUCCGGUCCUUGA 20 4028

HSV2-UL30-542 - CGUCGCCGAGGCCGUCU UGA 20 3412

HSV2-UL30-593 - CGGGAACGGGGACGAGGAUA 20 3463

HSV2-UL30-1194 + CGCGGCGGCCGCUCCGGAUA 20 4064

HSV2-UL30-762 - GCCGGCGGGGGCCGGCGCUA 20 3632

HSV2-UL30-664 - UACUCCAUGCGCAUCAUCUA 20 3534

HSV2-UL30-448 - CGCGUCGCCGUUCACGUCUA 20 3318

HSV2-UL30-518 - AUGACCUUCG UCAAGCAGUA 20 3388

HSV2-UL30-1217 + UAGAUGAUGCGCAUGGAGUA 20 4087

HSV2-UL30-1155 + GUCUGGGCCACGAUCACGUA 20 4025

HSV2-UL30-526 - AAGGUCCCGCUCGACGGGUA 20 3396

HSV2-UL30-539 - AUGGUGAACAUCGACAUGUA 20 3409

HSV2-UL30-647 - GUGGUGUGCAACUCGGUGUA 20 3517

HSV2-UL30-2870 - CCAGCUCCACG AGCG AU U U A 20 11224

HSV2-UL30-1359 + CGCAGGAUCCGAGCGAAAAC 20 4229

HSV2-UL30-1100 + UCUUGGCGUUAUUUCCAAAC 20 3970

HSV2-UL30-1400 + CCGGGUUGUCCAGGAUAAAC 20 4270

HSV2-UL30-643 - CG U CCU CCU CG ACAAGCAAC 20 3513

HSV2-UL30-590 - AGCGGCCGGGGGACGGGAAC 20 3460

HSV2-UL30-537 - G C AG C A AG AUCAAGGUGAAC 20 3407

HSV2-UL30-1273 + GCAAAG U CAAACACCACCAC 20 4143

HSV2-UL30-1263 + GGGAGAGCGUACUGAAGCAC 20 4133

HSV2-UL30-1407 + CGGGGCAAAAGUUGUCGCAC 20 4277

HSV2-UL30-649 - AACUCGGUGUACGGGU UCAC 20 3519

HSV2-UL30-1367 + GGACAGGUCGUAGAGCAGAC 20 4237

HSV2-UL30-604 - GAGGAGGUCGCGCGCGAGAC 20 3474

HSV2-UL30-1344 + CUGUCAAACUCCAGGACGAC 20 4214

HSV2-UL30-1372 + UCUUCCGGGCGUUCCGCGAC 20 4242

HSV2-UL30-638 - UGAGCAUCCUGCUGCGCGAC 20 3508

HSV2-UL30-525 - UCUACAAGGUCCCGCUCGAC 20 3395

HSV2-UL30-521 - ACAACAUCAUCAACUUCGAC 20 3391

HSV2-UL30-2880 + UGCGGGACAUCAGCUUCGAC 20 11234

HSV2-UL30-710 - AGGUCCCGUCCAUCAAGGAC 20 3580

HSV2-UL30-596 - AUAAGGACGACGACGAGGAC 20 3466

HSV2-UL30-600 - ACGAGGACGGGGACGAGGAC 20 3470

HSV2-UL30-1218 + GAUGCGCAUGGAGUACGGAC 20 4088

HSV2-UL30-627 - UCGCGCACCUGGAGGCGGAC 20 3497 HSV2-UL30-588 - AAGGGGAGCGGCCGGGGGAC 20 3458

HSV2-UL30-1160 + GGAUCCGGUCCUUGAUGGAC 20 4030

HSV2-UL30-1366 + GCUCGAGGGCGGUGGUGGAC 20 4236

HSV2-UL30-519 - UACGGCCCCGAGU UCGUGAC 20 3389

HSV2-UL30-665 - ACUCCAUGCGCAUCAUCUAC 20 3535

HSV2-UL30-1410 + GCACGAAGACGCGGUAGUAC 20 4280

HSV2-UL30-1156 + UCUGGGCCACGAUCACGUAC 20 4026

HSV2-UL30-527 - AGGUCCCGCUCGACGGGUAC 20 3397

HSV2-UL30-1270 + CCUGGAUGAUGCUGGGGUAC 20 4140

HSV2-UL30-648 - UGGUGUGCAACUCGGUGUAC 20 3518

HSV2-UL30-1275 + CACGGGGUCGACGUGAAACC 20 4145

HSV2-UL30-1401 + CGGGUUGUCCAGGAUAAACC 20 4271

HSV2-UL30-493 - UAACUGCACGGCGGACAACC 20 3363

HSV2-UL30-480 - CCGGU UUAUCCUGGACAACC 20 3350

HSV2-UL30-478 - GGGGCGUCGACGCCACCACC 20 3348

HSV2-UL30-1211 + UCUUGUCGCCCAUGGCCACC 20 4081

HSV2-UL30-554 - CU UCAAGUUUCUGCCGCACC 20 3424

HSV2-UL30-624 - GCCCGAGGCCGUCGCGCACC 20 3494

HSV2-UL30-650 - ACUCGGUGUACGGGUUCACC 20 3520

HSV2-UL30-605 - AGGAGGUCGCGCGCGAGACC 20 3475

HSV2-UL30-701 - CCAUCGGCGCAUCACCGACC 20 3571

HSV2-UL30-1196 + CG U A A A AC AG CAGGUCGACC 20 4066

HSV2-UL30-1281 + CGGAGGUGGGGUCGAGGACC 20 4151

HSV2-UL30-1219 + AUGCGCAUGGAGUACGGACC 20 4089

HSV2-UL30-628 - CGCGCACCUGGAGGCGGACC 20 3498

HSV2-UL30-520 - ACGGCCCCGAGUUCGUGACC 20 3390

HSV2-UL30-629 - GGAGGCGGACCGGGACUACC 20 3499

HSV2-UL30-1108 + GCCCCGGGCGAUGGCGUACC 20 3978

HSV2-UL30-611 - GGGCCGGCACGUUGGGUACC 20 3481

HSV2-UL30-1336 + GAAGUUGAUGAUGU UGUACC 20 4206

HSV2-UL30-1397 + CAG CCG A AG G U G AC AAACCC 20 4267

HSV2-UL30-481 - CGGUUUAUCCUGGACAACCC 20 3351

HSV2-UL30-1329 + CCCACACGCGGAACACACCC 20 4199

HSV2-UL30-564 - CUUCAUCCUGCCGGACACCC 20 3434

HSV2-UL30-1234 + CAGAAGACCGUGCUGCACCC 20 4104

HSV2-UL30-1282 + GGAGGUGGGGUCGAGGACCC 20 4152

HSV2-UL30-1109 + CCCCGGGCGAUGGCGUACCC 20 3979

HSV2-UL30-1398 + AGCCG AAGG U G ACAAACCCC 20 4268

HSV2-UL30-746 - UCCCGAGACGUGGCACCCCC 20 3616

HSV2-UL30-724 - GCCGUCGCAUGCCGACCCCC 20 3594

HSV2-UL30-725 - CCGUCGCAUGCCGACCCCCC 20 3595

HSV2-UL30-1374 + GGCCAGCUCGUCCUCCCCCC 20 4244

HSV2-UL30-723 - CUCCCCGG CCA AG CG CCCCC 20 3593

HSV2-UL30-1283 + GGUCGAGGACCCGGGCCCCC 20 4153

HSV2-UL30-1106 + CGUGUUGAGCGGAACGCCCC 20 3976

HSV2-UL30-1418 + AUGCCGCGGAACGACGCCCC 20 4288 HSV2-UL30-1294 + CGAGGCCCCGAAACCGCCCC 20 4164

HSV2-UL30-717 - GAGCUAGACGCCGCCGCCCC 20 3587

HSV2-UL30-722 - CCUCCCCGGCCAAGCGCCCC 20 3592

HSV2-UL30-663 - GCGGCCGGCAUGCGCGCCCC 20 3533

HSV2-UL30-1349 + GACGGGGGCCGGCAGGCCCC 20 4219

HSV2-UL30-1285 + GUACCCAACGUGCCGGCCCC 20 4155

HSV2-UL30-1286 + U CG U CCCCG U U CCCG U CCCC 20 4156

HSV2-UL30-1142 + GGGGGGGCGGGCUCGUCCCC 20 4012

HSV2-UL30-1105 + CCGUGUUGAGCGGAACGCCC 20 3975

HSV2-UL30-734 - UCCCGGGUACGCCAUCGCCC 20 3604

HSV2-UL30-1198 + CAGCAGGUCGACCAGGGCCC 20 4068

HSV2-UL30-688 - CAACCGCACCUCCAGGGCCC 20 3558

HSV2-UL30-616 - CGUUGGGUACCAGGGGGCCC 20 3486

HSV2-UL30-732 - CCGAGCUGGCGGAGGAUCCC 20 3602

HSV2-UL30-721 - CCCAGCGGCCCUGCCCUCCC 20 3591

HSV2-UL30-1116 + GGGGCUUGGACGCGCCUCCC 20 3986

HSV2-UL30-1123 + GGCAUGCGACGGCGUCUCCC 20 3993

HSV2-UL30-485 - GGCUGGUACCGCCUCAAGCC 20 3355

HSV2-UL30-510 - CUUUCCGGUCGCGGAACGCC 20 3380

HSV2-UL30-444 - UGGACGCCAUCACGCCCGCC 20 3314

HSV2-UL30-1307 + GGGUGAUGUUGAUGCCCGCC 20 4177

HSV2-UL30-1421 + ACUCGCGCAGGGCCGCCGCC 20 4291

HSV2-UL30-705 - CGCGUACACCAACAAGCGCC 20 3575

HSV2-UL30-573 - CAAGGAGGCGCCCAAGCGCC 20 3443

HSV2-UL30-455 - GCGCGAUCUCUGCGAGCGCC 20 3325

HSV2-UL30-555 - GCUUUCCGCCGUCGCGCGCC 20 3425

HSV2-UL30-733 - AUCCCGGGUACGCCAUCGCC 20 3603

HSV2-UL30-459 - GCGGCCCUGCGCGAGUCGCC 20 3329

HSV2-UL30-501 - UCGAUAUCGAAUGCAAGGCC 20 3371

HSV2-UL30-530 - GUACGGGCGCAUGAACGGCC 20 3400

HSV2-UL30-1290 + CU UCCCCCCG AGGCACGGCC 20 4160

HSV2-UL30-1380 + ACAUGGCCCCCUCGACGGCC 20 4250

HSV2-UL30-584 - CGGGGGGAAGGGGAGCGGCC 20 3454

HSV2-UL30-1393 + GCCGGCGCGUUCCCGCGGCC 20 4263

HSV2-UL30-672 - CGGCCUCACGGCCGCGGGCC 20 3542

HSV2-UL30-1314 + CACCCCGCGCUGCGCGGGCC 20 4184

HSV2-UL30-615 - ACGUUGGGUACCAGGGGGCC 20 3485

HSV2-UL30-1167 + A AU ACACCG U C AGG U G G G CC 20 4037

HSV2-UL30-1266 + UGAAGCACAGGUUGUGGGCC 20 4136

HSV2-UL30-1128 + UCUCCCGGGGGCGCUUGGCC 20 3998

HSV2-UL30-704 - CGACCCGGAGAGGGACAUCC 20 3574

HSV2-UL30-621 - CCUGUACCCCAGCAUCAUCC 20 3491

HSV2-UL30-731 - UCCGAGCUGGCGGAGGAUCC 20 3601

HSV2-UL30-479 - CGCCACCACCCGGUUUAUCC 20 3349

HSV2-UL30-1342 + CGAAU UCGCUGUCAAACUCC 20 4212

HSV2-UL30-618 - GGGUCCUCGACCCCACCUCC 20 3488 HSV2-UL30-686 - CGUUUAUCAACCGCACCUCC 20 3556

HSV2-UL30-545 - UCCCCGCCUACUACGCCUCC 20 3415

HSV2-UL30-1257 + GGUAGUCCCGGUCCGCCUCC 20 4127

HSV2-UL30-1115 + CGGGGCUUGGACGCGCCUCC 20 3985

HSV2-UL30-512 - ACCUCAGCGAUCUCGCCUCC 20 3382

HSV2-UL30-1310 + GCGCGACGGCGGAAAGCUCC 20 4180

HSV2-UL30-1255 + GGCCCCCCACCUCGAUCUCC 20 4125

HSV2-UL30-1176 + AGUCCUGGAUGUCCCUCUCC 20 4046

HSV2-UL30-1122 + CGGCAUGCGACGGCGUCUCC 20 3992

HSV2-UL30-1174 + CGGCGGUGAGGACAAAGUCC 20 4044

HSV2-UL30-1256 + CCUCGAUCUCCAGGUAGUCC 20 4126

HSV2-UL30-1092 + GCCGCGCGGCCACGUCG UCC 20 3962

HSV2-UL30-516 - CCUGCCGGCCCCCG UCGUCC 20 3386

HSV2-UL30-1434 + CAGAAGCGUGAUGACGGUCC 20 4304

HSV2-UL30-1399 + UGACAAACCCCGGGU UGUCC 20 4269

HSV2-UL30-1371 + UCUGGAUGACGAGGUCUUCC 20 4241

HSV2-UL30-470 - ACCGCGUCUUCGUGCGAAGC 20 3340

HSV2-UL30-1195 + CGGUAUCGUCGUAAAACAGC 20 4065

HSV2-UL30-1103 + U CACG C AG G CCG CCCCCAG C 20 3973

HSV2-UL30-1250 + UGGCCAGCCAGUCGCGCAGC 20 4120

HSV2-UL30-1251 + CGCGCAGCAGGAUGCUCAGC 20 4121

HSV2-UL30-507 - GGCCGGGGGGGAGGACGAGC 20 3377

HSV2-UL30-728 - CAAGCUGCUGG UGUCCGAGC 20 3598

HSV2-UL30-1331 + UGCGCCCGUACCCGUCGAGC 20 4201

HSV2-UL30-2862 + CCAUAAAUCGCUCGUGGAGC 20 11216

HSV2-UL30-1324 + GACGGCGUUCAGCUUGUAGC 20 4194

HSV2-UL30-1102 + CAGGGCCUUGAACGUCACGC 20 3972

HSV2-UL30-1088 + GCCGUAGCGCCGGCCCCCGC 20 3958

HSV2-UL30-443 - AUGGACGCCAUCACGCCCGC 20 3313

HSV2-UL30-671 - UGCCGCGGCCUCACGGCCGC 20 3541

HSV2-UL30-487 - ACCGCCUCAAGCCCGGCCGC 20 3357

HSV2-UL30-1134 + GCCGGGGAGGGCAGGGCCGC 20 4004

HSV2-UL30-752 - GCCGCGCGGCUCAGGGCCGC 20 3622

HSV2-UL30-1261 + GCGCGACGGCCUCGGGCCGC 20 4131

HSV2-UL30-547 - CCUCCGGGCCCGCGCAGCGC 20 3417

HSV2-UL30-1087 + AGUUUCCUCCGCCGUAGCGC 20 3957

HSV2-UL30-488 - GCCCGGCCGCGGGAACGCGC 20 3358

HSV2-UL30-655 - GCGCGUACGUGCACGCGCGC 20 3525

HSV2-UL30-1170 + GCUUGUUGGUGUACGCGCGC 20 4040

HSV2-UL30-708 - GCUCAUGGCCCGCCGCGCGC 20 3578

HSV2-UL30-471 - CGUGCGAAGCGGGCGCGCGC 20 3341

HSV2-UL30-1423 + GGCGCUCGCAGAGAUCGCGC 20 4293

HSV2-UL30-1419 + ACGCCCCCGGCGACUCGCGC 20 4289

HSV2-UL30-1300 + CCUUCUGGCCCGCAAGGCGC 20 4170

HSV2-UL30-1313 + CCGAUCACCCCGCGCUGCGC 20 4183

HSV2-UL30-1405 + CCCUCGUACUUCCUGAUCGC 20 4275 HSV2-UL30-1152 + GACCGUCUCCUCUACCUCGC 20 4022

HSV2-UL30-458 - GGCGGCCCUGCGCGAGUCGC 20 3328

HSV2-UL30-1358 + GUGGGACUCGGGGAGG UCGC 20 4228

HSV2-UL30-500 - UUCGAUAUCGAAUGCAAGGC 20 3370

HSV2-UL30-1271 + GAUGAUGCUGGGGUACAGGC 20 4141

HSV2-UL30-1232 + GGUGGCGGCCACGUGCAGGC 20 4102

HSV2-UL30-1318 + GGCCCGGAGGCGUAGUAGGC 20 4188

HSV2-UL30-1376 + AAGCACAUGAGCU UGUAGGC 20 4246

HSV2-UL30-529 - GGUACGGGCGCAUGAACGGC 20 3399

HSV2-UL30-1289 + CCUUCCCCCCGAGGCACGGC 20 4159

HSV2-UL30-1436 + AGCGUGAUGACGGUCCCGGC 20 4306

HSV2-UL30-1377 + ACAUGAGCUUGUAGGCCGGC 20 4247

HSV2-UL30-758 - GCCGCGGGGUUCGGGCCGGC 20 3628

HSV2-UL30-1348 + CCAGGACGACGGGGGCCGGC 20 4218

HSV2-UL30-1297 + ACCGCCCCUGGGUGUCCGGC 20 4167

HSV2-UL30-583 - UCGGGGGGAAGGGGAGCGGC 20 3453

HSV2-UL30-1392 + GGCCGGCGCGU UCCCGCGGC 20 4262

HSV2-UL30-1163 + GGACGGGACCUGCGCGCGGC 20 4033

HSV2-UL30-716 - AGAGGAGACGGUCGCGCGGC 20 3586

HSV2-UL30-662 - GCCGACUU UCCGGAGGCGGC 20 3532

HSV2-UL30-484 - CGGGGUUUGUCACCU UCGGC 20 3354

HSV2-UL30-1132 + GGCGCU UGGCCGGGGAGGGC 20 4002

HSV2-UL30-1184 + ACGCCUGCAGUCCCUCGGGC 20 4054

HSV2-UL30-756 - CAGGGCCGCGGGGUUCGGGC 20 3626

HSV2-UL30-1347 + AACUCCAGGACGACGGGGGC 20 4217

HSV2-UL30-608 - UCGCGCGCGAGACCGGGGGC 20 3478

HSV2-UL30-761 - GGGUUCGGGCCGGCGGGGGC 20 3631

HSV2-UL30-1141 + GGCAGGGCCGCUGGGGGGGC 20 4011

HSV2-UL30-1390 + GUCGGGGGGCGCGGU UGGGC 20 4260

HSV2-UL30-639 - CAUCCUGCUGCGCGACUGGC 20 3509

HSV2-UL30-557 - UCCGCCGUCGCGCGCCUGGC 20 3427

HSV2-UL30-693 - GCGCCCCGCAGAGGAGUGGC 20 3563

HSV2-UL30-1127 + GUCUCCCGGGGGCGCUUGGC 20 3997

HSV2-UL30-1222 + CGGACCGGGGGCGCGCAUGC 20 4092

HSV2-UL30-1267 + GUUGUGGGCCUGGAUGAUGC 20 4137

HSV2-UL30-1426 + CGCGCGGGGCACGGCACUGC 20 4296

HSV2-UL30-696 - ACCCCUGCCCGAGGGACUGC 20 3566

HSV2-UL30-737 - CUAUUACUUCUCGCACCUGC 20 3607

HSV2-UL30-498 - GGGGGCCAUGUGUGACCUGC 20 3368

HSV2-UL30-515 - UCUCGCCUCCAGGGGCCUGC 20 3385

HSV2-UL30-563 - CCAGAAGGGCUUCAUCCUGC 20 3433

HSV2-UL30-727 - G U CCA AG CCCCGCAAGCUGC 20 3597

HSV2-UL30-658 - GGCGGAGUUCGAUCAGCUGC 20 3528

HSV2-UL30-551 - UUGUGUGCAGGACUCGCUGC 20 3421

HSV2-UL30-517 - CAGCGAAUUCGAGAUGCUGC 20 3387

HSV2-UL30-679 - AGUACAUCGGCGUCAUCUGC 20 3549 HSV2-UL30-1303 + ACGUGAAGACGCGGAUCUGC 20 4173

HSV2-UL30-1188 + CGCGCCAGCCACUCCUCUGC 20 4058

HSV2-UL30-1284 + CCCCCUGGUACCCAACGUGC 20 4154

HSV2-UL30-1231 + UCACGGUGGCGGCCACGUGC 20 4101

HSV2-UL30-1306 + CUGGCCG UCGUAGAUGGUGC 20 4176

HSV2-UL30-550 - GAUCGGCGAGUAUUGUGUGC 20 3420

HSV2-UL30-560 - ACGUGCCUCCUGCGCCUUGC 20 3430

HSV2-UL30-1112 + CUCGGACACCAGCAGCU UGC 20 3982

HSV2-UL30-1368 + CGUAGAGCAGACAGGAGAUC 20 4238

HSV2-UL30-472 - ACAACU U U UGCCCCGCG AUC 20 3342

HSV2-UL30-1157 + CCACGAUCACGUACGGGAUC 20 4027

HSV2-UL30-685 - GCUCAUCAAGGGCGUGGAUC 20 3555

HSV2-UL30-1204 + U UUCGCACUCGAGCU UGAUC 20 4074

HSV2-UL30-689 - U UUUACGACGAUACCGUAUC 20 3559

HSV2-UL30-1338 + UUGUACCCGGUCACGAACUC 20 4208

HSV2-UL30-1355 + AGAUCGCUGAGGUGGGACUC 20 4225

HSV2-UL30-617 - CGGGUCCUCGACCCCACCUC 20 3487

HSV2-UL30-1183 + CCGAACGCCUGCAGUCCCUC 20 4053

HSV2-UL30-544 - AUCCCCGCCUACUACGCCUC 20 3414

HSV2-UL30-1223 + GCGCGCAUGCCGGCCGCCUC 20 4093

HSV2-UL30-1260 + UCCAGGUGCGCGACGGCCUC 20 4130

HSV2-UL30-575 - CAAGCGCCCGGCCGUGCCUC 20 3445

HSV2-UL30-1241 + UCGAGGAGGACGGCCUCCUC 20 4111

HSV2-UL30-1193 + GGCUAACGCGGCGGCCGCUC 20 4063

HSV2-UL30-749 - ACGACGUGGCCGCGCGGCUC 20 3619

HSV2-UL30-1327 + UGCGCUUCUGAAAGUGGCUC 20 4197

HSV2-UL30-1244 + CGGCCUCCUCGGGGGUGCUC 20 4114

HSV2-UL30-1175 + AAGUCCUGGAUGUCCCUCUC 20 4045

HSV2-UL30-1097 + UCCGGGGGGUGCCACGUCUC 20 3967

HSV2-UL30-1164 + UGAGCUUGUAAUACACCGUC 20 4034

HSV2-UL30-1383 + UCGAGGUUCCGAACGCCGUC 20 4253

HSV2-UL30-1091 + AGCCGCGCGGCCACGUCGUC 20 3961

HSV2-UL30-1413 + AGACGCGGUAGUACAGGGUC 20 4283

HSV2-UL30-1153 + UCUCCUCUACCUCGCGGGUC 20 4023

HSV2-UL30-553 - UGCAGGACUCGCUGCUGGUC 20 3423

HSV2-UL30-1333 + AGAUCUCCGUCAGCUUGGUC 20 4203

HSV2-UL30-1296 + CGAAACCGCCCCUGGGUGUC 20 4166

HSV2-UL30-1298 + CCGGCAGGAUGAAGCCCUUC 20 4168

HSV2-UL30-1430 + ACGGCGACGCGAUGGCCUUC 20 4300

HSV2-UL30-445 - CGGGACCGUCAUCACGCUUC 20 3315

HSV2-UL30-1370 + AUCUGGAUGACGAGGUCUUC 20 4240

HSV2-UL30-698 - CCGAGGGACUGCAGGCGU UC 20 3568

HSV2-UL30-755 - GGCUCAGGGCCGCGGGGUUC 20 3625

HSV2-UL30-659 - UCAGCUGCUGGCCGACU UUC 20 3529

HSV2-UL30-508 - GGAGGACGAGCUGGCCU UUC 20 3378

HSV2-UL30-569 - GGACACCCAGGGGCGGUU UC 20 3439 HSV2-UL30-1326 + UCUUGCUGCGCU UCUGAAAG 20 4196

HSV2-UL30-744 - UCACCGAGAGUCUGUUAAAG 20 3614

HSV2-UL30-469 - UACCGCGUCUUCGUGCGAAG 20 3339

HSV2-UL30-1415 + CCACCACCU CCGCCU CGAAG 20 4285

HSV2-UL30-581 - GGCCGUGCCUCGGGGGGAAG 20 3451

HSV2-UL30-613 - GCCGGCACGU UGGGUACCAG 20 3483

HSV2-UL30-566 - UCAUCCUGCCGGACACCCAG 20 3436

HSV2-UL30-720 - GGACGAGCCCGCCCCCCCAG 20 3590

HSV2-UL30-719 - GCUAGACGCCGCCGCCCCAG 20 3589

HSV2-UL30-514 - CUCAGCGAUCUCGCCUCCAG 20 3384

HSV2-UL30-691 - GUUAGCCGAGCGCCCCGCAG 20 3561

HSV2-UL30-1186 + GCCUGCAGUCCCUCGGGCAG 20 4056

HSV2-UL30-475 - CCGCGAUCAGGAAGUACGAG 20 3345

HSV2-UL30-1248 + UGCUCUGGGGGAUCCGCGAG 20 4118

HSV2-UL30-1330 + AUGCGCCCGUACCCGUCGAG 20 4200

HSV2-UL30-496 - CGGACAACCUGGCCGUCGAG 20 3366

HSV2-UL30-692 - CCGAGCGCCCCGCAGAGGAG 20 3562

HSV2-UL30-702 - GGCGCAUCACCGACCCGGAG 20 3572

HSV2-UL30-690 - CGACGAUACCGUAUCCGGAG 20 3560

HSV2-UL30-582 - UGCCUCGGGGGGAAGGGGAG 20 3452

HSV2-UL30-1104 + AAGUAAUAGUCCGUGU UGAG 20 3974

HSV2-UL30-713 - GGCCCAGACCCGCGAGGUAG 20 3583

HSV2-UL30-591 - GCGGCCGGGGGACGGGAACG 20 3461

HSV2-UL30-1191 + UGCGGGGCGCUCGGCUAACG 20 4061

HSV2-UL30-1328 + UCUGGCCGAUGUCCCACACG 20 4198

HSV2-UL30-1274 + CAA AG U CA AACACC ACCACG 20 4144

HSV2-UL30-449 - CCGUUCACGUCUACGGCACG 20 3319

HSV2-UL30-653 - UCUUCUGCCCUGCCUGCACG 20 3523

HSV2-UL30-1409 + GCCCGCUUCGCACGAAGACG 20 4279

HSV2-UL30-1302 + GCAGGAGGCACGUGAAGACG 20 4172

HSV2-UL30-745 - AGAGGUUUAUUCCCGAGACG 20 3615

HSV2-UL30-594 - CGAGGAUAAGGACGACGACG 20 3464

HSV2-UL30-1345 + UGUCAAACUCCAGGACGACG 20 4215

HSV2-UL30-747 - GUGGCACCCCCCGGACGACG 20 3617

HSV2-UL30-2881 + GCGGGACAUCAGCUUCGACG 20 11235

HSV2-UL30-597 - UAAGGACGACGACGAGGACG 20 3467

HSV2-UL30-601 - CGAGGACGGGGACGAGGACG 20 3471

HSV2-UL30-592 - GGGGGACGGGAACGGGGACG 20 3462

HSV2-UL30-598 - CGACGACGAGGACGGGGACG 20 3468

HSV2-UL30-1369 + GACAGGAGAUCUGGAUGACG 20 4239

HSV2-UL30-666 - CUCCAUGCGCAUCAUCUACG 20 3536

HSV2-UL30-1180 + UGCGCCGAUGGGCGUCUACG 20 4050

HSV2-UL30-473 - CCCCG CG AU C AG G A AG U ACG 20 3343

HSV2-UL30-1252 + UCAGCAGGCUCUCGCGUACG 20 4122

HSV2-UL30-651 - CUCGGUGUACGGGUUCACCG 20 3521

HSV2-UL30-606 - GGAGGUCGCGCGCGAGACCG 20 3476 HSV2-UL30-1220 + UGCGCAUGGAGUACGGACCG 20 4090

HSV2-UL30-482 - GGUUUAUCCUGGACAACCCG 20 3352

HSV2-UL30-1089 + CCCCGCCGGCCCGAACCCCG 20 3959

HSV2-UL30-619 - CGGGUUUCACGUCGACCCCG 20 3489

HSV2-UL30-641 - GAUCCCCCAGAGCACCCCCG 20 3511

HSV2-UL30-1287 + GGCCGCUCCCCUUCCCCCCG 20 4157

HSV2-UL30-735 - CCCGGGUACGCCAUCGCCCG 20 3605

HSV2-UL30-1212 + GCCCAUGGCCACCAGGCCCG 20 4082

HSV2-UL30-623 - UACGCUCUCCCUGCGGCCCG 20 3493

HSV2-UL30-694 - GCUGGCGCGACCCCUGCCCG 20 3564

HSV2-UL30-1117 + GGGCUUGGACGCGCCUCCCG 20 3987

HSV2-UL30-1124 + GCAUGCGACGGCGUCUCCCG 20 3994

HSV2-UL30-1391 + GUUGGGCCGGCGCGU UCCCG 20 4261

HSV2-UL30-460 - CG G CCC U G CG CG AG U CG CCG 20 3330

HSV2-UL30-541 - CAAGCUGAACGCCGUCGCCG 20 3411

HSV2-UL30-502 - CGAUAUCGAAUGCAAGGCCG 20 3372

HSV2-UL30-1214 + GGCCCGCGGCCGUGAGGCCG 20 4084

HSV2-UL30-531 - UACGGGCGCAUGAACGGCCG 20 3401

HSV2-UL30-670 - GUGCCGCGGCCUCACGGCCG 20 3540

HSV2-UL30-486 - UACCGCCUCAAGCCCGGCCG 20 3356

HSV2-UL30-585 - GGGGGGAAGGGGAGCGGCCG 20 3455

HSV2-UL30-751 - GGCCGCGCGGCUCAGGGCCG 20 3621

HSV2-UL30-1129 + CUCCCGGGGGCGCUUGGCCG 20 3999

HSV2-UL30-1417 + AGUGG UCCGCGGAGAUGCCG 20 4287

HSV2-UL30-668 - UCCAUUUUCGUU UUGUGCCG 20 3538

HSV2-UL30-463 - GUCGUUCCGCGGCAUCUCCG 20 3333

HSV2-UL30-1093 + CCGCGCGGCCACGUCGUCCG 20 3963

HSV2-UL30-1416 + CUCCGCCUCGAAGUGGUCCG 20 4286

HSV2-UL30-462 - UCGCCGGGGGCGUCGU UCCG 20 3332

HSV2-UL30-546 - GCCUCCGGGCCCGCGCAGCG 20 3416

HSV2-UL30-712 - GAUCGUGGCCCAGACCCGCG 20 3582

HSV2-UL30-1090 + CCCCGCGGCCCUGAGCCGCG 20 3960

HSV2-UL30-753 - CCG CG CG G CU CAG GGCCGCG 20 3623

HSV2-UL30-748 - CCCCGGACGACGUGGCCGCG 20 3618

HSV2-UL30-548 - CUCCGGGCCCGCGCAGCGCG 20 3418

HSV2-UL30-602 - CGAGGACGGGGACGAGCGCG 20 3472

HSV2-UL30-1169 + CGCUUGUUGGUGUACGCGCG 20 4039

HSV2-UL30-1424 + GCGCUCGCAGAGAUCGCGCG 20 4294

HSV2-UL30-1161 + UGAUGGACGGGACCUGCGCG 20 4031

HSV2-UL30-1422 + AGGCGCUCGCAGAGAUCGCG 20 4292

HSV2-UL30-1406 + CCUCGUACUUCCUGAUCGCG 20 4276

HSV2-UL30-1225 + GCACGUACGCGCGCG UCGCG 20 4095

HSV2-UL30-715 - AGGUAGAGGAGACGGUCGCG 20 3585

HSV2-UL30-1319 + GCCCGGAGGCG UAGUAGGCG 20 4189

HSV2-UL30-759 - CCGCGGGGUUCGGGCCGGCG 20 3629

HSV2-UL30-684 - CAAGAUGCUCAUCAAGGGCG 20 3554 HSV2-UL30-1387 + CCGAACGCCGUCGGGGGGCG 20 4257

HSV2-UL30-1312 + GCCGAUCACCCCGCGCUGCG 20 4182

HSV2-UL30-680 - GUACAUCGGCGUCAUCUGCG 20 3550

HSV2-UL30-1189 + GCGCCAGCCACUCCUCUGCG 20 4059

HSV2-UL30-1113 + UCGGACACCAGCAGCUUGCG 20 3983

HSV2-UL30-630 - CCGGGACUACCUGGAGAUCG 20 3500

HSV2-UL30-1404 + CCCCUCGUACU UCCUGAUCG 20 4274

HSV2-UL30-711 - CCGGAUCCCGUACGUGAUCG 20 3581

HSV2-UL30-1205 + U UCGCACUCGAGCUUGAUCG 20 4075

HSV2-UL30-1339 + UGUACCCGG UCACGAACUCG 20 4209

HSV2-UL30-1356 + GAUCGCUGAGGUGGGACUCG 20 4226

HSV2-UL30-1151 + CGACCGUCUCCUCUACCUCG 20 4021

HSV2-UL30-576 - AAGCGCCCGGCCGUGCCUCG 20 3446

HSV2-UL30-1242 + CGAGGAGGACGGCCUCCUCG 20 4112

HSV2-UL30-1147 + GGGCGGCGGCGUCUAGCUCG 20 4017

HSV2-UL30-640 - UGCGAAAGCAGAUCCGCUCG 20 3510

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-1381 + GUUAAACUCGACGUCGCUCG 20 4251

HSV2-UL30-1361 + AAAACAGGAGGAUGUGCUCG 20 4231

HSV2-UL30-1226 + GCGUCGCGAGGAGCAUCUCG 20 4096

HSV2-UL30-1384 + CGAGGUUCCGAACGCCG UCG 20 4254

HSV2-UL30-494 - GGCGGACAACCUGGCCGUCG 20 3364

HSV2-UL30-509 - CGAGCUGGCCUU UCCGGUCG 20 3379

HSV2-UL30-1280 + GAAACCCGGAGGUGGGGUCG 20 4150

HSV2-UL30-1320 + CGUAGUAGGCGGGGAUGUCG 20 4190

HSV2-UL30-1293 + GCUUGGGCGCCUCCUUGUCG 20 4163

HSV2-UL30-1237 + UGGCGGCCUGUUGCUUGUCG 20 4107

HSV2-UL30-464 - CAUCUCCGCGGACCACU UCG 20 3334

HSV2-UL30-1431 + CGGCGACGCGAUGGCCU UCG 20 4301

HSV2-UL30-699 - CGAGGGACUGCAGGCGU UCG 20 3569

HSV2-UL30-570 - GACACCCAGGGGCGGUUUCG 20 3440

HSV2-UL30-451 - GUACUU UUACAUGAACAAGG 20 3321

HSV2-UL30-572 - GUUUCGGGGCCUCGACAAGG 20 3442

HSV2-UL30-645 - G C A AC AG GCCGCCAUCAAGG 20 3515

HSV2-UL30-1360 + AGGAUCCGAGCGAAAACAGG 20 4230

HSV2-UL30-614 - CCGGCACGU UGGGUACCAGG 20 3484

HSV2-UL30-1301 + UCUGGCCCGCAAGGCGCAGG 20 4171

HSV2-UL30-1165 + GCU UGUAAUACACCGUCAGG 20 4035

HSV2-UL30-476 - CGCGAUCAGGAAGUACGAGG 20 3346

HSV2-UL30-642 - CCCCCAGAGCACCCCCGAGG 20 3512

HSV2-UL30-603 - GGACGGGGACGAGCGCGAGG 20 3473

HSV2-UL30-631 - GGACUACCUGGAGAUCGAGG 20 3501

HSV2-UL30-497 - GGACAACCUGGCCGUCGAGG 20 3367

HSV2-UL30-1238 + CGGCCUGUUGCUUGUCGAGG 20 4108

HSV2-UL30-465 - CUCCGCGG ACCACU UCG AGG 20 3335

HSV2-UL30-1277 + GUCGACGUGAAACCCGGAGG 20 4147 HSV2-UL30-661 - GCUGGCCGACUU UCCGGAGG 20 3531

HSV2-UL30-453 - UUACAUGAACAAGGCGGAGG 20 3323

HSV2-UL30-467 - GGACCACUUCGAGGCGGAGG 20 3337

HSV2-UL30-626 - GGCCGUCGCGCACCUGGAGG 20 3496

HSV2-UL30-1352 + UGGAGGCGAGAUCGCUGAGG 20 4222

HSV2-UL30-1395 + UCCCGCGGCCGGGCUUGAGG 20 4265

HSV2-UL30-1317 + GGGCCCGGAGGCGUAG UAGG 20 4187

HSV2-UL30-492 - CGUCGAGUUUAACUGCACGG 20 3362

HSV2-UL30-1229 + GCGGCCGAUGGUCGUCACGG 20 4099

HSV2-UL30-1346 + GUCAAACUCCAGGACGACGG 20 4216

HSV2-UL30-1309 + GCCCGCCAGGCGCGCGACGG 20 4179

HSV2-UL30-2882 + CGGGACAUCAGCUUCGACGG 20 11236

HSV2-UL30-763 - GGCGGGGGCCGGCGCUACGG 20 3633

HSV2-UL30-607 - GAGGUCGCGCGCGAGACCGG 20 3477

HSV2-UL30-1221 + GCGCAUGGAGUACGGACCGG 20 4091

HSV2-UL30-1276 + GGGGUCGACGUGAAACCCGG 20 4146

HSV2-UL30-1315 + CCCGCGCUGCGCGGGCCCGG 20 4185

HSV2-UL30-1118 + GGCUUGGACGCGCCUCCCGG 20 3988

HSV2-UL30-1125 + CAUGCGACGGCGUCUCCCGG 20 3995

HSV2-UL30-1435 + AAGCGUGAUGACGGUCCCGG 20 4305

HSV2-UL30-461 - GGCCCUGCGCGAGUCGCCGG 20 3331

HSV2-UL30-503 - GAUAUCGAAUGCAAGGCCGG 20 3373

HSV2-UL30-586 - GGGGGAAGGGGAGCGGCCGG 20 3456

HSV2-UL30-757 - GGCCGCGGGGUUCGGGCCGG 20 3627

HSV2-UL30-1094 + CGCGCGGCCACGUCGUCCGG 20 3964

HSV2-UL30-660 - GCUGCUGGCCGACU UUCCGG 20 3530

HSV2-UL30-1192 + GGGGCGCUCGGCUAACGCGG 20 4062

HSV2-UL30-1162 + UGGACGGGACCUGCGCGCGG 20 4032

HSV2-UL30-1148 + CGGCGGCGUCUAGCUCGCGG 20 4018

HSV2-UL30-452 - CU UUUACAUGAACAAGGCGG 20 3322

HSV2-UL30-466 - CGCGGACCACU UCGAGGCGG 20 3336

HSV2-UL30-764 - GGGGGCCGGCGCUACGGCGG 20 3634

HSV2-UL30-760 - CGCGGGGUUCGGGCCGGCGG 20 3630

HSV2-UL30-1364 + GAGGAUGUGCUCGAGGGCGG 20 4234

HSV2-UL30-1146 + GGGCUCGUCCCCUGGGGCGG 20 4016

HSV2-UL30-457 - UCUCUGCGAGCGCCUGGCGG 20 3327

HSV2-UL30-730 - GCUGGUGUCCGAGCUGGCGG 20 3600

HSV2-UL30-681 - UACAUCGGCGUCAUCUGCGG 20 3551

HSV2-UL30-1206 + UCGCACUCGAGCUUGAUCGG 20 4076

HSV2-UL30-577 - AGCGCCCGGCCGUGCCUCGG 20 3447

HSV2-UL30-1243 + GAGGAGGACGGCCUCCUCGG 20 4113

HSV2-UL30-1385 + GAGGU UCCGAACGCCGUCGG 20 4255

HSV2-UL30-1432 + GGCGACGCGAUGGCCU UCGG 20 4302

HSV2-UL30-1172 + CGGGUGUCUGCUCAGUUCGG 20 4042

HSV2-UL30-477 - GCGAUCAGGAAGUACGAGGG 20 3347

HSV2-UL30-1363 + CAGGAGGAUGUGCUCGAGGG 20 4233 HSV2-UL30-1150 + GGCGUCUAGCUCGCGGAGGG 20 4020

HSV2-UL30-2883 + GGGACAUCAGCUUCGACGGG 20 11237

HSV2-UL30-1402 + GUUGUCCAGGAUAAACCGGG 20 4272

HSV2-UL30-726 - UCGCAUGCCGACCCCCCGGG 20 3596

HSV2-UL30-1119 + GCU UGGACGCGCCUCCCGGG 20 3989

HSV2-UL30-504 - AUAUCGAAUGCAAGGCCGGG 20 3374

HSV2-UL30-1095 + GCGCGGCCACGUCGUCCGGG 20 3965

HSV2-UL30-1207 + CGCACUCGAGCUUGAUCGGG 20 4077

HSV2-UL30-578 - GCGCCCGGCCGUGCCUCGGG 20 3448

HSV2-UL30-1386 + AGGUUCCGAACGCCGUCGGG 20 4256

HSV2-UL30-567 - UCCUGCCGGACACCCAGGGG 20 3437

HSV2-UL30-505 - UAUCGAAUGCAAGGCCGGGG 20 3375

HSV2-UL30-1130 + CCGGGGGCGCU UGGCCGGGG 20 4000

HSV2-UL30-1357 + CGCUGAGGUGGGACUCGGGG 20 4227

HSV2-UL30-1208 + ACUCGAGCUUGAUCGGGGGG 20 4078

HSV2-UL30-506 - CGAAUGCAAGGCCGGGGGGG 20 3376

HSV2-UL30-1140 + GGGCAGGGCCGCUGGGGGGG 20 4010

HSV2-UL30-741 - CUUCUCGCACCUGCUGGGGG 20 3611

HSV2-UL30-1145 + GGCGGGCUCGUCCCCUGGGG 20 4015

HSV2-UL30-1139 + GGAGGGCAGGGCCGCUGGGG 20 4009

HSV2-UL30-2864 + AAAUCGCUCGUGGAGCUGGG 20 11218

HSV2-UL30-1138 + GGGAGGGCAGGGCCGCUGGG 20 4008

HSV2-UL30-657 - GUACGUGCACGCGCGCUGGG 20 3527

HSV2-UL30-635 - UACCUGGAGAUCGAGGUGGG 20 3505

HSV2-UL30-1236 + GU UGCACACCACCUUGAUGG 20 4106

HSV2-UL30-625 - CGAGGCCGUCGCGCACCUGG 20 3495

HSV2-UL30-1350 + GGGGGCCGGCAGGCCCCUGG 20 4220

HSV2-UL30-1199 + CAGGUCGACCAGGGCCCUGG 20 4069

HSV2-UL30-456 - CGAUCUCUGCGAGCGCCUGG 20 3326

HSV2-UL30-556 - UUCCGCCGUCGCGCGCCUGG 20 3426

HSV2-UL30-673 - CCUCACGGCCGCGGGCCUGG 20 3543

HSV2-UL30-729 - GCUGCUGGUGUCCGAGCUGG 20 3599

HSV2-UL30-1137 + GGGGAGGGCAGGGCCGCUGG 20 4007

HSV2-UL30-740 - UUACU UCUCGCACCUGCUGG 20 3610

HSV2-UL30-1247 + CCUCCUCGGGGGUGCUCUGG 20 4117

HSV2-UL30-620 - GU UUCACGUCGACCCCGUGG 20 3490

HSV2-UL30-634 - CUACCUGGAGAUCGAGGUGG 20 3504

HSV2-UL30-468 - CCACUUCGAGGCGGAGG UGG 20 3338

HSV2-UL30-1230 + GCCGAUGGUCGUCACGGUGG 20 4100

HSV2-UL30-1365 + GAUGUGCUCGAGGGCGGUGG 20 4235

HSV2-UL30-1403 + GUCCAGGAUAAACCGGG UGG 20 4273

HSV2-UL30-1209 + G G A AC AG CG CG CG CG AG A U G 20 4079

HSV2-UL30-1144 + GGGGGCGGGCUCGUCCCCUG 20 4014

HSV2-UL30-622 - GCUUCAGUACGCUCUCCCUG 20 3492

HSV2-UL30-1136 + CGGGGAGGGCAGGGCCGCUG 20 4006

HSV2-UL30-1351 + CCCUGGAGGCGAGAUCGCUG 20 4221 HSV2-UL30-1269 + UGUGGGCCUGGAUGAUGCUG 20 4139

HSV2-UL30-739 - AUUACUUCUCGCACCUGCUG 20 3609

HSV2-UL30-678 - AAGUACAUCGGCGUCAUCUG 20 3548

HSV2-UL30-1187 + UCGCGCCAGCCACUCCUCUG 20 4057

HSV2-UL30-1246 + GCCUCCUCGGGGGUGCUCUG 20 4116

HSV2-UL30-1213 + CCACCAGGCCCGCGGCCGUG 20 4083

HSV2-UL30-532 - GCCGGGGUGUGU UCCGCGUG 20 3402

HSV2-UL30-1311 + ACGGCGGAAAGCUCCAGGUG 20 4181

HSV2-UL30-633 - ACUACCUGGAGAUCGAGGUG 20 3503

HSV2-UL30-1279 + CGACG U G AAACCCG G AGG U G 20 4149

HSV2-UL30-1200 + CGACCAGGGCCCUGGAGGUG 20 4070

HSV2-UL30-1173 + GUCUGCUCAGUUCGGCGGUG 20 4043

HSV2-UL30-1305 + GCUGGCCGUCGUAGAUGGUG 20 4175

HSV2-UL30-559 - CACGUGCCUCCUGCGCCU UG 20 3429

HSV2-UL30-1111 + GCUCGG ACACCAGCAGCU UG 20 3981

HSV2-UL30-1394 + CGU UCCCGCGGCCGGGCUUG 20 4264

HSV2-UL30-1264 + GCGUACUGAAGCACAGGUUG 20 4134

HSV2-UL30-534 - GUGUUCCGCGUGUGGGACAU 20 3404

HSV2-UL30-677 - AUCGCCAAG A A A A AG U ACAU 20 3547

HSV2-UL30-654 - GCCGCCACCGUGACGACCAU 20 3524

HSV2-UL30-700 - CCGUCCUCGUAGACGCCCAU 20 3570

HSV2-UL30-675 - GCCGCGGGCCUGGUGGCCAU 20 3545

HSV2-UL30-1179 + CGGGUCGGUGAUGCGCCGAU 20 4049

HSV2-UL30-454 - UGAACAAGGCGGAGGUGGAU 20 3324

HSV2-UL30-549 - CCCGCGCAGCGCGGGGUGAU 20 3419

HSV2-UL30-1203 + UUU UCGCACUCGAGCUUGAU 20 4073

HSV2-UL30-646 - CAUCAAGGUGGUGUGCAACU 20 3516

HSV2-UL30-1337 + GU UGUACCCGGUCACGAACU 20 4207

HSV2-UL30-1354 + GAGAUCGCUGAGGUGGGACU 20 4224

HSV2-UL30-1295 + GAGGCCCCGAAACCGCCCCU 20 4165

HSV2-UL30-1143 + GGGGGGCGGGCUCGUCCCCU 20 4013

HSV2-UL30-1182 + CCCGAACGCCUGCAGUCCCU 20 4052

HSV2-UL30-1322 + CU UGUCCUUCAAGACGGCCU 20 4192

HSV2-UL30-1259 + CUCCAGGUGCGCGACGGCCU 20 4129

HSV2-UL30-574 - CCAAGCGCCCGGCCGUGCCU 20 3444

HSV2-UL30-1240 + GUCGAGGAGGACGGCCUCCU 20 4110

HSV2-UL30-1110 + CCCGGGAUCCUCCGCCAGCU 20 3980

HSV2-UL30-1202 + CU UGGCGAUGAGCAGCAGCU 20 4072

HSV2-UL30-1332 + CU UGUAGAUCUCCGUCAGCU 20 4202

HSV2-UL30-2871 + CAUAAAUCGCUCGUGGAGCU 20 11225

HSV2-UL30-1135 + CCGGGGAGGGCAGGGCCGCU 20 4005

HSV2-UL30-656 - CGCGUACGUGCACGCGCGCU 20 3526

HSV2-UL30-1291 + CCGAGGCACGGCCGGGCGCU 20 4161

HSV2-UL30-1190 + CCACUCCUCUGCGGGGCGCU 20 4060

HSV2-UL30-1126 + CGGCGUCUCCCGGGGGCGCU 20 3996

HSV2-UL30-511 - CACAUCCUCCUGUU UUCGCU 20 3381 HSV2-UL30-1114 + CACCAGCAGCUUGCGGGGCU 20 3984

HSV2-UL30-1268 + U UGUGGGCCUGGAUGAUGCU 20 4138

HSV2-UL30-738 - UAUUACUUCUCGCACCUGCU 20 3608

HSV2-UL30-1099 + UAACAGACUCUCGGUGAUCU 20 3969

HSV2-UL30-1098 + AAACCUCUUUAACAGACUCU 20 3968

HSV2-UL30-1245 + GGCCUCCUCGGGGGUGCUCU 20 4115

HSV2-UL30-1096 + GUCCGGGGGGUGCCACGUCU 20 3966

HSV2-UL30-1154 + CUCCUCUACCUCGCGGGUCU 20 4024

HSV2-UL30-446 - GGGACCGUCAUCACGCU UCU 20 3316

HSV2-UL30-1201 + GACGCCGAUGUACUUU UUCU 20 4071

HSV2-UL30-1224 + GCCGGCCGCCUCCGGAAAGU 20 4094

HSV2-UL30-1316 + CGCGGGCCCGGAGGCGUAGU 20 4186

HSV2-UL30-1414 + CGUUUCGUAAUAGUACACGU 20 4284

HSV2-UL30-609 - GAGACCGGGGGCCGGCACGU 20 3479

HSV2-UL30-1253 + CAGCAGGCUCUCGCG UACGU 20 4123

HSV2-UL30-1254 + CCU UCACGAAGAACAGCCGU 20 4124

HSV2-UL30-1382 + CUCGAGGUUCCGAACGCCGU 20 4252

HSV2-UL30-1408 + G C A A A AG UUGUCGCACAGGU 20 4278

HSV2-UL30-1166 + CU UGUAAUACACCGUCAGGU 20 4036

HSV2-UL30-632 - GACUACCUGGAGAUCGAGGU 20 3502

HSV2-UL30-1278 + UCGACGUGAAACCCGGAGGU 20 4148

HSV2-UL30-1353 + GGAGGCGAGAUCGCUGAGGU 20 4223

HSV2-UL30-1388 + ACGCCGUCGGGGGGCGCGGU 20 4258

HSV2-UL30-1412 + AAG ACGCGG U AG UACAGGG U 20 4282

HSV2-UL30-1177 + CUGGAUGUCCCUCUCCGGGU 20 4047

HSV2-UL30-1120 + GGACGCGCCUCCCGGGGGGU 20 3990

HSV2-UL30-552 - GUGCAGGACUCGCUGCUGGU 20 3422

HSV2-UL30-533 - CCGGGGUGUGUUCCGCGUGU 20 3403

HSV2-UL30-1325 + GCUGGAGAGUUUGACCUUGU 20 4195

HSV2-UL30-1375 + AUCGAAGCACAUGAGCUUGU 20 4245

HSV2-UL30-1168 + CAGGUGGGCCAGGCGCU UGU 20 4038

HSV2-UL30-1265 + CGUACUGAAGCACAGGU UGU 20 4135

HSV2-UL30-483 - AACCCGGGGUUUGUCACCUU 20 3353

HSV2-UL30-1429 + AACGGCGACGCGAUGGCCUU 20 4299

HSV2-UL30-1292 + CGAGGCACGGCCGGGCGCUU 20 4162

HSV2-UL30-1171 + GCGCGGGUGUCUGCUCAGUU 20 4041

HSV2-UL30-610 - AGACCGGGGGCCGGCACGU U 20 3480

HSV2-UL30-697 - CCCGAGGGACUGCAGGCGU U 20 3567

HSV2-UL30-490 - CCGCGCCCCCCGACGGCGU U 20 3360

HSV2-UL30-1389 + CGCCGUCGGGGGGCGCGGUU 20 4259

HSV2-UL30-754 - CGGCUCAGGGCCGCGGGGUU 20 3624

HSV2-UL30-743 - GUGACGU UCAAGGCCCUGUU 20 3613

HSV2-UL30-568 - CGGACACCCAGGGGCGGUU U 20 3438 Table 9A provides exemplary targeting domains for knocking out the UL30 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality, and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 9A

Table 9B provides exemplary targeting domains for knocking out the UL30 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 9B

HSV2-U L30-2934 - ACGCCCCCGCGGAGCAGCGCAC 22 11288

HSV2-U L30-50 - CGGUGCGGACCAUGCCCCCG 20 3082

HSV2-U L30-2935 - CGUACUACAGCGAGUGCG 18 11289

HSV2-U L30-2936 - ACGUACUACAGCGAGUGCG 19 11290

HSV2-UL30-264 - UACG UACUACAGCGAGUGCG 20 4669

HSV2-UL30-2937 - AU ACGUACUACAGCGAGUGCG 21 11291

HSV2-U L30-2938 - CAU ACGUACUACAGCGAGUGCG 22 11292

HSV2-UL30-2939 - CCAU ACGUACUACAGCGAGUGCG 23 11293

HSV2-UL30-2940 - AGCCGGCGGCUCGGGCGGCGU 21 11294

HSV2-U L30-2941 - AAGCCGGCGGCUCGGGCGGCGU 22 11295

Table 9C provides exemplary targeting domains for knocking out the UL30 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 9C

Table 9D provides exemplary targeting domains for knocking out the UL30 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 9D

HSV2-UL30-364 + AGCGAACGCGGGGCGAUAAA 20 4726

HSV2-UL30-2946 + CAGCGAACGCGGGGCGAUAAA 21 11300

HSV2-UL30-2947 + CCAGCGAACGCGGGGCGAUAAA 22 11301

HSV2-UL30-2948 + UCCAGCGAACGCGGGGCGAUAAA 23 11302

HSV2-UL30-2949 + GUCCAGCGAACGCGGGGCGAUAAA 24 11303

HSV2-UL30-2950 + CGGGGGCGUCCUCGUCCA 18 11304

HSV2-UL30-2951 + GCGGGGGCGUCCUCGUCCA 19 11305

HSV2-UL30-361 + CGCGGGGGCGUCCUCGUCCA 20 4724

HSV2-UL30-2952 + CCGCGGGGGCGUCCUCGUCCA 21 11306

HSV2-UL30-2953 + UCCGCGGGGGCGUCCUCGUCCA 22 11307

HSV2-UL30-2954 + CUCCGCGGGGGCGUCCUCGUCCA 23 11308

HSV2-UL30-2955 + GCUCCGCGGGGGCGUCCUCGUCCA 24 11309

HSV2-UL30-2956 + GGCUGCGUUCCGGUCUGA 18 11310

HSV2-UL30-2957 + UGGCUGCGUUCCGGUCUGA 19 11311

HSV2-UL30-369 + UUGGCUGCGUUCCGGUCUGA 20 4731

HSV2-UL30-2958 + UUUGGCUGCGUUCCGGUCUGA 21 11312

HSV2-UL30-2959 + CUUUGGCUGCGUUCCGGUCUGA 22 11313

HSV2-UL30-2960 + CCUUUGGCUGCGUUCCGGUCUGA 23 11314

HSV2-UL30-2961 + GCCUUUGGCUGCGUUCCGGUCUGA 24 11315

HSV2-UL30-2962 + CGUCCUCGUCCAGCGAAC 18 11316

HSV2-UL30-2963 + GCGUCCUCGUCCAGCGAAC 19 11317

HSV2-UL30-362 + GGCGUCCUCGUCCAGCGAAC 20 4725

HSV2-UL30-2964 + GGGCGUCCUCGUCCAGCGAAC 21 11318

HSV2-UL30-2965 + GGGGCGUCCUCGUCCAGCGAAC 22 11319

HSV2-UL30-2966 + GGGGGCGUCCUCGUCCAGCGAAC 23 11320

HSV2-UL30-2967 + CGGGGGCGUCCUCGUCCAGCGAAC 24 11321

HSV2-UL30-2968 + CUCCAGGAUGUCGUACAC 18 11322

HSV2-UL30-2969 + GCUCCAGGAUGUCGUACAC 19 11323

HSV2-UL30-340 + UGCUCCAGGAUGUCGUACAC 20 4712

HSV2-UL30-2970 + GUGCUCCAGGAUGUCGUACAC 21 11324

HSV2-UL30-2971 + CGUGCUCCAGGAUGUCGUACAC 22 11325

HSV2-UL30-2972 + ACGUGCUCCAGGAUGUCGUACAC 23 11326

HSV2-UL30-2973 + CACGUGCUCCAGGAUGUCGUACAC 24 11327

HSV2-UL30-2974 + GAAG CCCU CCG GG CCCAC 18 11328

HSV2-UL30-2975 + AG AAG CCCU CCG GGCCCAC 19 11329

HSV2-UL30-350 + CAG AAG CCCU CCG GG CCCAC 20 4718

HSV2-UL30-2976 + CCAGAAGCCCUCCGGGCCCAC 21 11330

HSV2-UL30-2977 + GCCAGAAGCCCUCCGGGCCCAC 22 11331

HSV2-UL30-2978 + GGCCAGAAGCCCUCCGGGCCCAC 23 11332

HSV2-UL30-2979 + CGGCCAGAAGCCCUCCGGGCCCAC 24 11333

HSV2-UL30-2980 + ACGGUGGGGUCGAACCCC 18 11334

HSV2-UL30-2981 + GACGGUGGGGUCGAACCCC 19 11335

HSV2-UL30-345 + UGACGGUGGGGUCGAACCCC 20 4715

HSV2-UL30-2982 + GUGACGGUGGGGUCGAACCCC 21 11336

HSV2-UL30-2983 + GGUG ACGGUGGGGUCGAACCCC 22 11337

HSV2-UL30-2984 + CGGUGACGGUGGGGUCGAACCCC 23 11338 HSV2-UL30-2985 + ACGGUGACGGUGGGGUCGAACCCC 24 11339

HSV2-UL30-2986 + AGCCGCCGGCUUCCCCCC 18 11340

HSV2-UL30-2987 + GAGCCGCCGGCUUCCCCCC 19 11341

HSV2-UL30-118 + CGAGCCGCCGGCUUCCCCCC 20 3229

HSV2-UL30-2988 + CCGAGCCGCCGGCUUCCCCCC 21 11342

HSV2-UL30-2989 + CCCGAGCCGCCGGCUUCCCCCC 22 11343

HSV2-UL30-2990 + GCCCGAGCCGCCGGCUUCCCCCC 23 11344

HSV2-UL30-2991 + CGCCCGAGCCGCCGGCUUCCCCCC 24 11345

HSV2-UL30-2992 + GAGCCGCCGGCUUCCCCC 18 11346

HSV2-UL30-2993 + CGAGCCGCCGGCUUCCCCC 19 11347

HSV2-UL30-117 + CCGAGCCGCCGGCUUCCCCC 20 3228

HSV2-UL30-2994 + CCCGAGCCGCCGGCUUCCCCC 21 11348

HSV2-UL30-2995 + GCCCGAGCCGCCGGCUUCCCCC 22 11349

HSV2-UL30-2996 + CGCCCGAGCCGCCGGCUUCCCCC 23 11350

HSV2-UL30-2997 + CCGCCCGAGCCGCCGGCUUCCCCC 24 11351

HSV2-UL30-2998 + CGAGCCGCCGGCUUCCCC 18 11352

HSV2-UL30-2999 + CCGAGCCGCCGGCUUCCCC 19 11353

HSV2-UL30-383 + CCCGAGCCGCCGGCUUCCCC 20 4738

HSV2-UL30-3000 + GCCCGAGCCGCCGGCUUCCCC 21 11354

HSV2-UL30-3001 + CGCCCGAGCCGCCGGCUUCCCC 22 11355

HSV2-UL30-3002 + CCGCCCGAGCCGCCGGCUUCCCC 23 11356

HSV2-UL30-3003 + GCCGCCCGAGCCGCCGGCUUCCCC 24 11357

HSV2-UL30-3004 + AUGGCGCUGAGCCGGCCC 18 11358

HSV2-UL30-3005 + UAUGGCGCUGAGCCGGCCC 19 11359

HSV2-UL30-367 + GUAUGGCGCUGAGCCGGCCC 20 4729

HSV2-UL30-3006 + CGUAUGGCGCUGAGCCGGCCC 21 11360

HSV2-UL30-3007 + ACGUAUGGCGCUGAGCCGGCCC 22 11361

HSV2-UL30-3008 + UACGUAUGGCGCUGAGCCGGCCC 23 11362

HSV2-UL30-3009 + GUACGUAUGGCGCUGAGCCGGCCC 24 11363

HSV2-UL30-3010 + GCCGUCUGGGUGGCUCCC 18 11364

HSV2-UL30-3011 + UGCCGUCUGGGUGGCUCCC 19 11365

HSV2-UL30-107 + GUGCCGUCUGGGUGGCUCCC 20 3219

HSV2-UL30-3012 + GGUGCCGUCUGGGUGGCUCCC 21 11366

HSV2-UL30-3013 + CGGUGCCGUCUGGGUGGCUCCC 22 11367

HSV2-UL30-3014 + GCGGUGCCGUCUGGGUGGCUCCC 23 11368

HSV2-UL30-3015 + GGCGGUGCCGUCUGGGUGGCUCCC 24 11369

HSV2-UL30-3016 + UGCCGUCUGGGUGGCUCC 18 11370

HSV2-UL30-3017 + GUGCCGUCUGGGUGGCUCC 19 11371

HSV2-UL30-375 + GGUGCCGUCUGGGUGGCUCC 20 4735

HSV2-UL30-3018 + CGGUGCCGUCUGGGUGGCUCC 21 11372

HSV2-UL30-3019 + GCGGUGCCGUCUGGGUGGCUCC 22 11373

HSV2-UL30-3020 + GGCGGUGCCGUCUGGGUGGCUCC 23 11374

HSV2-UL30-3021 + CGGCGGUGCCGUCUGGGUGGCUCC 24 11375

HSV2-UL30-3022 + ACCCCGGUGCGCUGCUCC 18 11376

HSV2-UL30-3023 + GACCCCGGUGCGCUGCUCC 19 11377

HSV2-UL30-358 + GGACCCCGGUGCGCUGCUCC 20 4723 HSV2-UL30-3024 + UGGACCCCGGUGCGCUGCUCC 21 11378

HSV2-UL30-3025 + GUGGACCCCGGUGCGCUGCUCC 22 11379

HSV2-UL30-3026 + CGUGGACCCCGGUGCGCUGCUCC 23 11380

HSV2-UL30-3027 + UCGUGGACCCCGGUGCGCUGCUCC 24 11381

HSV2-UL30-3028 + AAAAAACCCAGACGCCGC 18 11382

HSV2-UL30-3029 + CAAAAAACCCAGACGCCGC 19 11383

HSV2-UL30-382 + GCAAAAAACCCAGACGCCGC 20 4737

HSV2-UL30-3030 + GGCAAAAAACCCAGACGCCGC 21 11384

HSV2-UL30-3031 + G G G CAAAAAACCCAG ACG CCG C 22 11385

HSV2-UL30-3032 + GGGGCAAAAAACCCAGACGCCGC 23 11386

HSV2-UL30-3033 + G G G G G CAAAAAACCCAG ACG CCG C 24 11387

HSV2-UL30-3034 + CCCGGUGCGCUGCUCCGC 18 11388

HSV2-UL30-3035 + CCCCGGUGCGCUGCUCCGC 19 11389

HSV2-UL30-82 + ACCCCGGUGCGCUGCUCCGC 20 3208

HSV2-UL30-3036 + GACCCCGGUGCGCUGCUCCGC 21 11390

HSV2-UL30-3037 + GGACCCCGGUGCGCUGCUCCGC 22 11391

HSV2-UL30-3038 + UGGACCCCGGUGCGCUGCUCCGC 23 11392

HSV2-UL30-3039 + GUGGACCCCGGUGCGCUGCUCCGC 24 11393

HSV2-UL30-3040 + UACACCU UAGGGGCGCGC 18 11394

HSV2-UL30-3041 + GUACACCUUAGGGGCGCGC 19 11395

HSV2-UL30-76 + AGUACACCUUAGGGGCGCGC 20 3095

HSV2-UL30-3042 + CAGUACACCUUAGGGGCGCGC 21 11396

HSV2-UL30-3043 + GCAGUACACCUUAGGGGCGCGC 22 11397

HSV2-UL30-3044 + CGCAG UACACCU UAGGGGCGCGC 23 11398

HSV2-UL30-3045 + CCGCAGUACACCUUAGGGGCGCGC 24 11399

HSV2-UL30-3046 + GUAUGGCGCUGAGCCGGC 18 11400

HSV2-UL30-3047 + CGUAUGGCGCUGAGCCGGC 19 11401

HSV2-UL30-366 + ACGUAUGGCGCUGAGCCGGC 20 4728

HSV2-UL30-3048 + UACGUAUGGCGCUGAGCCGGC 21 11402

HSV2-UL30-3049 + GU ACGUAUGGCGCUGAGCCGGC 22 11403

HSV2-UL30-3050 + AGU ACGUAUGGCGCUGAGCCGGC 23 11404

HSV2-UL30-3051 + UAGUACGUAUGGCGCUGAGCCGGC 24 11405

HSV2-UL30-3052 + AGGCGCAAGCGACGCGGC 18 11406

HSV2-UL30-3053 + CAGGCGCAAGCGACGCGGC 19 11407

HSV2-UL30-348 + ACAGGCGCAAGCGACGCGGC 20 4716

HSV2-UL30-3054 + CACAGGCGCAAGCGACGCGGC 21 11408

HSV2-UL30-3055 + CCACAGGCGCAAGCGACGCGGC 22 11409

HSV2-UL30-3056 + CCCACAGGCGCAAGCGACGCGGC 23 11410

HSV2-UL30-3057 + CCCCACAGGCGCAAGCGACGCGGC 24 11411

HSV2-UL30-3058 + GGUGGGGUCGAACCCCUC 18 11412

HSV2-UL30-3059 + CGGUGGGGUCGAACCCCUC 19 11413

HSV2-UL30-63 + ACGGUGGGGUCGAACCCCUC 20 3087

HSV2-UL30-3060 + GACGGUGGGGUCGAACCCCUC 21 11414

HSV2-UL30-3061 + UGACGGUGGGGUCGAACCCCUC 22 11415

HSV2-UL30-3062 + GUGACGGUGGGGUCGAACCCCUC 23 11416

HSV2-UL30-3063 + GGUGACGGUGGGGUCGAACCCCUC 24 11417 HSV2-UL30-3064 + GGCGUCCAUAAAUCGCUC 18 11418

HSV2-UL30-3065 + UGGCGUCCAUAAAUCGCUC 19 11419

HSV2-UL30-2593 + AUGGCGUCCAUAAAUCGCUC 20 5300

HSV2-UL30-3066 + GCGCCGGAGGCGGCCGUC 18 11420

HSV2-UL30-3067 + CGCGCCGGAGGCGGCCGUC 19 11421

HSV2-UL30-357 + GCGCGCCGGAGGCGGCCGUC 20 4722

HSV2-UL30-3068 + GGCGCGCCGGAGGCGGCCGUC 21 11422

HSV2-UL30-3069 + GGGCGCGCCGGAGGCGGCCGUC 22 11423

HSV2-UL30-3070 + GGGGCGCGCCGGAGGCGGCCGUC 23 11424

HSV2-UL30-3071 + AGGGGCGCGCCGGAGGCGGCCGUC 24 11425

HSV2-UL30-3072 + GCUUCCCCCCGGGGGAAG 18 11426

HSV2-UL30-3073 + GGCUUCCCCCCGGGGGAAG 19 11427

HSV2-UL30-388 + CGGCUUCCCCCCGGGGGAAG 20 4739

HSV2-UL30-3074 + CCGGCUUCCCCCCGGGGGAAG 21 11428

HSV2-UL30-3075 + GCCGGCUUCCCCCCGGGGGAAG 22 11429

HSV2-UL30-3076 + CGCCGGCUUCCCCCCGGGGGAAG 23 11430

HSV2-UL30-3077 + CCGCCGGCUUCCCCCCGGGGGAAG 24 11431

HSV2-UL30-3078 + CGUUCCGGUCUGAGCGAG 18 11432

HSV2-UL30-3079 + GCGUUCCGGUCUGAGCGAG 19 11433

HSV2-UL30-370 + UGCGU UCCGGUCUGAGCGAG 20 4732

HSV2-UL30-3080 + CUGCGUUCCGGUCUGAGCGAG 21 11434

HSV2-UL30-3081 + GCUGCGU UCCGGUCUGAGCGAG 22 11435

HSV2-UL30-3082 + GGCUGCGU UCCGGUCUGAGCGAG 23 11436

HSV2-UL30-3083 + UGGCUGCGUUCCGGUCUGAGCGAG 24 11437

HSV2-UL30-3084 + CAUAAAUCGCUCGUGGAG 18 11438

HSV2-UL30-3085 + CCAUAAAUCGCUCGUGGAG 19 11439

HSV2-UL30-3086 + UCCAUAAAUCGCUCGUGGAG 20 11440

HSV2-UL30-3087 + GUCCAUAAAUCGCUCGUGGAG 21 11441

HSV2-UL30-3088 + CGUCCAUAAAUCGCUCGUGGAG 22 11442

HSV2-UL30-3089 + GCGUCCAUAAAUCGCUCGUGGAG 23 11443

HSV2-UL30-3090 + GGCGUCCAUAAAUCGCUCGUGGAG 24 11444

HSV2-UL30-3091 + GUCCUCGUCCAGCGAACG 18 11445

HSV2-UL30-3092 + CGUCCUCGUCCAGCGAACG 19 11446

HSV2-UL30-85 + GCGUCCUCGUCCAGCGAACG 20 3098

HSV2-UL30-3093 + GGCGUCCUCGUCCAGCGAACG 21 11447

HSV2-UL30-3094 + GGGCGUCCUCGUCCAGCGAACG 22 11448

HSV2-UL30-3095 + GGGGCGUCCUCGUCCAGCGAACG 23 11449

HSV2-UL30-3096 + GGGGGCGUCCUCGUCCAGCGAACG 24 11450

HSV2-UL30-3097 + UCCAGGAUGUCGUACACG 18 11451

HSV2-UL30-3098 + CUCCAGGAUGUCGUACACG 19 11452

HSV2-UL30-56 + GCUCCAGGAUGUCGUACACG 20 3194

HSV2-UL30-3099 + UGCUCCAGGAUGUCGUACACG 21 11453

HSV2-UL30-3100 + GUGCU CCAG G AU G U CGUACACG 22 11454

HSV2-UL30-3101 + CGUGCUCCAGGAUGUCGUACACG 23 11455

HSV2-UL30-3102 + ACGUGCUCCAGGAUGUCGUACACG 24 11456

HSV2-UL30-3103 + AAGCCCUCCGGGCCCACG 18 11457 HSV2-UL30-3104 + GAAGCCCUCCGGGCCCACG 19 11458

HSV2-UL30-71 + AGAAGCCCUCCGGGCCCACG 20 3201

HSV2-UL30-3105 + CAGAAGCCCUCCGGGCCCACG 21 11459

HSV2-UL30-3106 + CCAGAAGCCCUCCGGGCCCACG 22 11460

HSV2-UL30-3107 + GCCAGAAGCCCUCCGGGCCCACG 23 11461

HSV2-UL30-3108 + GGCCAGAAGCCCUCCGGGCCCACG 24 11462

HSV2-UL30-3109 + ACGUGGAAGACGGUGACG 18 11463

HSV2-UL30-3110 + CACGUGGAAGACGGUGACG 19 11464

HSV2-UL30-342 + ACACGUGGAAGACGGUGACG 20 4713

HSV2-UL30-3111 + UACACGUGGAAGACGGUGACG 21 11465

HSV2-UL30-3112 + GUACACGUGGAAGACGGUGACG 22 11466

HSV2-UL30-3113 + CGU ACACGUGGAAGACGGUGACG 23 11467

HSV2-UL30-3114 + UCGUACACGUGGAAGACGGUGACG 24 11468

HSV2-UL30-3115 + GCCGCCGGCUUCCCCCCG 18 11469

HSV2-UL30-3116 + AGCCGCCGGCUUCCCCCCG 19 11470

HSV2-UL30-119 + GAGCCGCCGGCUUCCCCCCG 20 3230

HSV2-UL30-3117 + CGAGCCGCCGGCUUCCCCCCG 21 11471

HSV2-UL30-3118 + CCGAGCCGCCGGCUUCCCCCCG 22 11472

HSV2-UL30-3119 + CCCGAGCCGCCGGCUUCCCCCCG 23 11473

HSV2-UL30-3120 + GCCCGAGCCGCCGGCUUCCCCCCG 24 11474

HSV2-UL30-3121 + CCCCGGUGCGCUGCUCCG 18 11475

HSV2-UL30-3122 + ACCCCGGUGCGCUGCUCCG 19 11476

HSV2-UL30-81 + GACCCCGGUGCGCUGCUCCG 20 3207

HSV2-UL30-3123 + GGACCCCGGUGCGCUGCUCCG 21 11477

HSV2-UL30-3124 + UGGACCCCGGUGCGCUGCUCCG 22 11478

HSV2-UL30-3125 + GUGGACCCCGGUGCGCUGCUCCG 23 11479

HSV2-UL30-3126 + CGUGGACCCCGGUGCGCUGCUCCG 24 11480

HSV2-UL30-3127 + GCCCUCCGGGCCCACGCG 18 11481

HSV2-UL30-3128 + AGCCCUCCGGGCCCACGCG 19 11482

HSV2-UL30-352 + AAGCCCUCCGGGCCCACGCG 20 4719

HSV2-UL30-3129 + GAAGCCCUCCGGGCCCACGCG 21 11483

HSV2-UL30-3130 + AGAAGCCCUCCGGGCCCACGCG 22 11484

HSV2-UL30-3131 + CAGAAGCCCUCCGGGCCCACGCG 23 11485

HSV2-UL30-3132 + CCAGAAGCCCUCCGGGCCCACGCG 24 11486

HSV2-UL30-3133 + GUACACCUUAGGGGCGCG 18 11487

HSV2-UL30-3134 + AGUACACCUUAGGGGCGCG 19 11488

HSV2-UL30-355 + CAGUACACCUUAGGGGCGCG 20 4721

HSV2-UL30-3135 + GCAGUACACCUUAGGGGCGCG 21 11489

HSV2-UL30-3136 + CGCAGUACACCU UAGGGGCGCG 22 11490

HSV2-UL30-3137 + CCGCAGUACACCUUAGGGGCGCG 23 11491

HSV2-UL30-3138 + CCCGCAGUACACCUUAGGGGCGCG 24 11492

HSV2-UL30-3139 + GCUGUAGUACGUAUGGCG 18 11493

HSV2-UL30-3140 + CGCUGUAGUACGUAUGGCG 19 11494

HSV2-UL30-365 + UCGCUGUAGUACGUAUGGCG 20 4727

HSV2-UL30-3141 + CUCGCUGUAGUACGUAUGGCG 21 11495

HSV2-UL30-3142 + ACUCGCUGUAGUACGUAUGGCG 22 11496 HSV2-UL30-3143 + CACUCGCUGUAGUACGUAUGGCG 23 11497

HSV2-UL30-3144 + GCACUCGCUGUAGUACGUAUGGCG 24 11498

HSV2-UL30-3145 + GCGUCCAUAAAUCGCUCG 18 11499

HSV2-UL30-3146 + GGCGUCCAUAAAUCGCUCG 19 11500

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-3147 + AUGGCGUCCAUAAAUCGCUCG 21 11501

HSV2-UL30-3148 + GAUGGCGUCCAUAAAUCGCUCG 22 11502

HSV2-UL30-3149 + UGAUGGCGUCCAUAAAUCGCUCG 23 11503

HSV2-UL30-3150 + GUGAUGGCGUCCAUAAAUCGCUCG 24 11504

HSV2-UL30-3151 + GUUCCGGUCUGAGCGAGG 18 11505

HSV2-UL30-3152 + CGUUCCGGUCUGAGCGAGG 19 11506

HSV2-UL30-95 + GCGUUCCGGUCUGAGCGAGG 20 3104

HSV2-UL30-3153 + UGCGUUCCGGUCUGAGCGAGG 21 11507

HSV2-UL30-3154 + CUGCGUUCCGGUCUGAGCGAGG 22 11508

HSV2-UL30-3155 + GCUGCGUUCCGGUCUGAGCGAGG 23 11509

HSV2-UL30-3156 + GGCUGCGUUCCGGUCUGAGCGAGG 24 11510

HSV2-UL30-3157 + CCGCCGGCUUCCCCCCGG 18 11511

HSV2-UL30-3158 + GCCGCCGGCUUCCCCCCGG 19 11512

HSV2-UL30-120 + AGCCGCCGGCUUCCCCCCGG 20 3231

HSV2-UL30-3159 + GAGCCGCCGGCUUCCCCCCGG 21 11513

HSV2-UL30-3160 + CGAGCCGCCGGCUUCCCCCCGG 22 11514

HSV2-UL30-3161 + CCGAGCCGCCGGCUUCCCCCCGG 23 11515

HSV2-UL30-3162 + CCCGAGCCGCCGGCUUCCCCCCGG 24 11516

HSV2-UL30-3163 + AAGACGGUGACGGUGGGG 18 11517

HSV2-UL30-3164 + GAAGACGGUGACGGUGGGG 19 11518

HSV2-UL30-344 + GG AAGACGGUGACGGUGGGG 20 4714

HSV2-UL30-3165 + UGGAAGACGGUGACGGUGGGG 21 11519

HSV2-UL30-3166 + GUGG AAGACGGUGACGGUGGGG 22 11520

HSV2-UL30-3167 + CGUGGAAGACGGUGACGGUGGGG 23 11521

HSV2-UL30-3168 + ACGUGGAAGACGGUGACGGUGGGG 24

11522

HSV2-UL30-3169 + GGCUCCCCGGGGGUUGUG 18 11523

HSV2-UL30-3170 + UGGCUCCCCGGGGGUUGUG 19 11524

HSV2-UL30-113 + GUGGCUCCCCGGGGGUUGUG 20 3224

HSV2-UL30-3171 + GGUGGCUCCCCGGGGGUUGUG 21 11525

HSV2-UL30-3172 + GGGUGGCUCCCCGGGGGUUGUG 22 11526

HSV2-UL30-3173 + UGGGUGGCUCCCCGGGGGUUGUG 23 11527

HSV2-UL30-3174 + CUGGGUGGCUCCCCGGGGGUUGUG 24

11528

HSV2-UL30-3175 + GUGGCUCCCCGGGGGUUG 18 11529

HSV2-UL30-3176 + GGUGGCUCCCCGGGGGUUG 19 11530

HSV2-UL30-111 + GGGUGGCUCCCCGGGGGUUG 20 3222

HSV2-UL30-3177 + UGGGUGGCUCCCCGGGGGUUG 21 11531

HSV2-UL30-3178 + CUGGGUGGCUCCCCGGGGGUUG 22 11532

HSV2-UL30-3179 + UCUGGGUGGCUCCCCGGGGGUUG 23 11533 HSV2-UL30-3180 + GUCUGGGUGGCUCCCCGGGGGUUG 24

11534

HSV2-UL30-3181 + UGAGCGAGGUGGGGGUUG 18 11535

HSV2-UL30-3182 + CUGAGCGAGGUGGGGGUUG 19 11536

HSV2-UL30-373 + UCUGAGCGAGGUGGGGGUUG 20 4733

HSV2-UL30-3183 + GUCUGAGCGAGGUGGGGGUUG 21 11537

HSV2-UL30-3184 + GGUCUGAGCGAGGUGGGGGUUG 22 11538

HSV2-UL30-3185 + CGGUCUGAGCGAGGUGGGGGUUG 23 11539

HSV2-UL30-3186 + CCGGUCUGAGCGAGGUGGGGGUUG 24

11540

HSV2-UL30-3187 + CCCCCCCGCAGUACACCU 18 11541

HSV2-UL30-3188 + UCCCCCCCGCAGUACACCU 19 11542

HSV2-UL30-353 + GUCCCCCCCGCAGUACACCU 20 4720

HSV2-UL30-3189 + CGUCCCCCCCGCAGUACACCU 21 11543

HSV2-UL30-3190 + UCGUCCCCCCCGCAGUACACCU 22 11544

HSV2-UL30-3191 + CUCGUCCCCCCCGCAGUACACCU 23 11545

HSV2-UL30-3192 + GCUCGUCCCCCCCGCAGUACACCU 24 11546

HSV2-UL30-3193 + CGGUGGGGUCGAACCCCU 18 11547

HSV2-UL30-3194 + ACGGUGGGGUCGAACCCCU 19 11548

HSV2-UL30-62 + GACGGUGGGGUCGAACCCCU 20 3086

HSV2-UL30-3195 + UG ACGGUGGGGUCGAACCCCU 21 11549

HSV2-UL30-3196 + GUGACGGUGGGGUCGAACCCCU 22 11550

HSV2-UL30-3197 + GGUGACGGUGGGGUCGAACCCCU 23 11551

HSV2-UL30-3198 + CGGUG ACGGUGGGGUCGAACCCCU 24 11552

HSV2-UL30-3199 + GACGCGGCCAGAAGCCCU 18 11553

HSV2-UL30-3200 + CGACGCGGCCAGAAGCCCU 19 11554

HSV2-UL30-349 + GCGACGCGGCCAGAAGCCCU 20 4717

HSV2-UL30-3201 + AGCGACGCGGCCAGAAGCCCU 21 11555

HSV2-UL30-3202 + AAGCGACGCGGCCAGAAGCCCU 22 11556

HSV2-UL30-3203 + CAAGCGACGCGGCCAGAAGCCCU 23 11557

HSV2-UL30-3204 + GCAAGCGACGCGGCCAGAAGCCCU 24 11558

HSV2-UL30-3205 + CUUUGGCUGCGUUCCGGU 18 11559

HSV2-UL30-3206 + CCUUUGGCUGCGUUCCGGU 19 11560

HSV2-UL30-368 + GCCUUUGGCUGCGUUCCGGU 20 4730

HSV2-UL30-3207 + GGCCUUUGGCUGCGUUCCGGU 21 11561

HSV2-UL30-3208 + GGGCCUUUGGCUGCGUUCCGGU 22 11562

HSV2-UL30-3209 + AGGGCCUUUGGCUGCGUUCCGGU 23 11563

HSV2-UL30-3210 + GAGGGCCUUUGGCUGCGUUCCGGU 24 11564

HSV2-UL30-3211 + UGGCUCCCCGGGGGU UGU 18 11565

HSV2-UL30-3212 + GUGGCUCCCCGGGGGUUGU 19 11566

HSV2-UL30-112 + GGUGGCUCCCCGGGGGU UGU 20 3223

HSV2-UL30-3213 + GGGUGGCUCCCCGGGGGUUGU 21 11567

HSV2-UL30-3214 + UGGGUGGCUCCCCGGGGGUUGU 22 11568

HSV2-UL30-3215 + CUGGGUGGCUCCCCGGGGGUUGU 23 11569

HSV2-UL30-3216 + UCUGGGUGGCUCCCCGGGGGUUGU 24

11570 HSV2-UL30-3217 + CCCCCCGCAGUACACCUU 18 11571

HSV2-UL30-3218 + CCCCCCCGCAG U ACACCU U 19 11572

HSV2-UL30-73 + UCCCCCCCGCAGUACACCUU 20 3203

HSV2-UL30-3219 + G U CCCCCCCG CAG U ACACCU U 21 11573

HSV2-UL30-3220 + CGUCCCCCCCGCAGUACACCUU 22 11574

HSV2-UL30-3221 + U CG U CCCCCCCGCAG U ACACCU U 23 11575

HSV2-UL30-3222 + CUCGUCCCCCCCGCAGUACACCUU 24 11576

HSV2-UL30-3223 + GGUGGCUCCCCGGGGGUU 18 11577

HSV2-UL30-3224 + GGGUGGCUCCCCGGGGGUU 19 11578

HSV2-UL30-378 + UGGGUGGCUCCCCGGGGGUU 20 4736

HSV2-UL30-3225 + CUGGGUGGCUCCCCGGGGGUU 21 11579

HSV2-UL30-3226 + UCUGGGUGGCUCCCCGGGGGUU 22 11580

HSV2-UL30-3227 + GUCUGGGUGGCUCCCCGGGGGUU 23 11581

HSV2-UL30-3228 + CGUCUGGGUGGCUCCCCGGGGGUU 24 11582

HSV2-UL30-3229 - CCCCCGCGGAGCAGCGCA 18 11583

HSV2-UL30-3230 - GCCCCCGCGGAGCAGCGCA 19 11584

HSV2-UL30-270 - CGCCCCCGCGGAGCAGCGCA 20 4674

HSV2-UL30-3231 - ACGCCCCCGCGGAGCAGCGCA 21 11585

HSV2-UL30-3232 - GACGCCCCCGCGGAGCAGCGCA 22 11586

HSV2-UL30-3233 - GGACGCCCCCGCGGAGCAGCGCA 23 11587

HSV2-UL30-3234 - AGGACGCCCCCGCGGAGCAGCGCA 24 11588

HSV2-UL30-3235 - ACCCCCACCUCGCUCAGA 18 11589

HSV2-UL30-3236 - AACCCCCACCU CG CUCAG A 19 11590

HSV2-UL30-260 - CAACCCCCACCUCGCUCAGA 20 4666

HSV2-UL30-3237 - ACAACCCCCACCU CG CU CAG A 21 11591

HSV2-UL30-3238 - UACAACCCCCACCUCGCUCAGA 22 11592

HSV2-UL30-3239 - CUACAACCCCCACCUCGCUCAGA 23 11593

HSV2-UL30-3240 - UCUACAACCCCCACCUCGCUCAGA 24 11594

HSV2-UL30-3241 - UACGACAUCCUGGAGCAC 18 11595

HSV2-UL30-3242 - GUACGACAUCCUGGAGCAC 19 11596

HSV2-UL30-291 - UGUACGACAUCCUGGAGCAC 20 4685

HSV2-UL30-3243 - GUGUACGACAUCCUGGAGCAC 21 11597

HSV2-UL30-3244 - CGUGUACGACAUCCUGGAGCAC 22 11598

HSV2-UL30-3245 - ACGUGUACGACAUCCUGGAGCAC 23 11599

HSV2-UL30-3246 - CACGUGUACGACAUCCUGGAGCAC 24 11600

HSV2-UL30-3247 - CCCCCACCUCGCUCAGAC 18 11601

HSV2-UL30-3248 - ACCCCCACCU CG CU CAG AC 19 11602

HSV2-UL30-19 - AACCCCCACCUCGCUCAGAC 20 3174

HSV2-UL30-3249 - CAACCCCCACCU CG CU CAG AC 21 11603

HSV2-UL30-3250 - ACAACCCCCACCUCGCUCAGAC 22 11604

HSV2-UL30-3251 - UACAACCCCCACCUCGCUCAGAC 23 11605

HSV2-UL30-3252 - CUACAACCCCCACCUCGCUCAGAC 24 11606

HSV2-UL30-3253 - GCCCCGCGUUCGCUGGAC 18 11607

HSV2-UL30-3254 - CGCCCCGCGUUCGCUGGAC 19 11608

HSV2-UL30-267 - UCGCCCCGCGUUCGCUGGAC 20 4672

HSV2-UL30-3255 - AUCGCCCCGCGUUCGCUGGAC 21 11609 HSV2-UL30-3256 - UAUCGCCCCGCGUUCGCUGGAC 22 11610

HSV2-UL30-3257 - UUAUCGCCCCGCGUUCGCUGGAC 23 11611

HSV2-UL30-3258 - UUUAUCGCCCCGCGUUCGCUGGAC 24 11612

HSV2-UL30-3259 - U U U U GCCCCCCACAACCC 18 11613

HSV2-UL30-3260 - U U U U U G CCCCCCACAACCC 19 11614

HSV2-UL30-255 - U U U U U UG CCCCCCACAACCC 20 4664

HSV2-UL30-3261 - GUUUUUUG CCCCCCACAACCC 21 11615

HSV2-UL30-3262 - GGUU UUUUGCCCCCCACAACCC 22 11616

HSV2-UL30-3263 - GGGUUUUUUG CCCCCCACAACCC 23 11617

HSV2-UL30-3264 - UGGGUUUUUUGCCCCCCACAACCC 24 11618

HSV2-UL30-3265 - U U U GCCCCCCACAACCCC 18 11619

HSV2-UL30-3266 - U U U U GCCCCCCACAACCCC 19 11620

HSV2-UL30-14 - U U U U U G CCCCCCACAACCCC 20 3170

HSV2-UL30-3267 - U U U U U U GCCCCCCACAACCCC 21 11621

HSV2-UL30-3268 - GUUUUUUGCCCCCCACAACCCC 22 11622

HSV2-UL30-3269 - GG U U U U U UG CCCCCCACAACCCC 23 11623

HSV2-UL30-3270 - GGGUUUUUUG CCCCCCACAACCCC 24 11624

HSV2-UL30-3271 - UUGCCCCCCACAACCCCC 18 11625

HSV2-UL30-3272 - U U U G CCCCCCACAACCCCC 19 11626

HSV2-UL30-15 - U U U U G CCCCCCACAACCCCC 20 3171

HSV2-UL30-3273 - U U U U U G CCCCCCACAACCCCC 21 11627

HSV2-UL30-3274 - U U U U U UG CCCCCCACAACCCCC 22 11628

HSV2-UL30-3275 - GUUUUUUG CCCCCCACAACCCCC 23 11629

HSV2-UL30-3276 - GGU U U U U UGCCCCCCACAACCCCC 24 11630

HSV2-UL30-3277 - CUGGACGAGGACGCCCCC 18 11631

HSV2-UL30-3278 - GCUGGACGAGGACGCCCCC 19 11632

HSV2-UL30-268 - CGCUGGACGAGGACGCCCCC 20 4673

HSV2-UL30-3279 - UCGCUGGACGAGGACGCCCCC 21 11633

HSV2-UL30-3280 - UUCGCUGGACGAGGACGCCCCC 22 11634

HSV2-UL30-3281 - GUUCGCUGGACGAGGACGCCCCC 23 11635

HSV2-UL30-3282 - CGUUCGCUGGACGAGGACGCCCCC 24 11636

HSV2-UL30-3283 - GGUGCGGACCAUGCCCCC 18 11637

HSV2-UL30-3284 - CGGUGCGGACCAUGCCCCC 19 11638

HSV2-UL30-287 - GCGGUGCGGACCAUGCCCCC 20 4683

HSV2-UL30-3285 - GGCGGUGCGGACCAUGCCCCC 21 11639

HSV2-UL30-3286 - GGGCGGUGCGGACCAUGCCCCC 22 11640

HSV2-UL30-3287 - GGGGCGGUGCGGACCAUGCCCCC 23 11641

HSV2-UL30-3288 - UGGGGCGGUGCGGACCAUGCCCCC 24 11642

HSV2-UL30-3289 - GGCGGCCCGGCUUCCCCC 18 11643

HSV2-UL30-3290 - GGGCGGCCCGGCUUCCCCC 19 11644

HSV2-UL30-3 - CGGGCGGCCCGGCUUCCCCC 20 3159

HSV2-UL30-3291 - GCGGGCGGCCCGGCUUCCCCC 21 11645

HSV2-UL30-3292 - CGCGGGCGGCCCGGCUUCCCCC 22 11646

HSV2-UL30-3293 - CCGCGGGCGGCCCGGCUUCCCCC 23 11647

HSV2-UL30-3294 - GCCGCGGGCGGCCCGGCUUCCCCC 24 11648

HSV2-UL30-3295 - GGGCGGCCCGGCUUCCCC 18 11649 HSV2-UL30-3296 - CGGGCGGCCCGGCUUCCCC 19 11650

HSV2-UL30-2 - GCGGGCGGCCCGGCUUCCCC 20 3158

HSV2-UL30-3297 - CGCGGGCGGCCCGGCUUCCCC 21 11651

HSV2-UL30-3298 - CCGCGGGCGGCCCGGCUUCCCC 22 11652

HSV2-UL30-3299 - GCCGCGGGCGGCCCGGCUUCCCC 23 11653

HSV2-UL30-3300 - UGCCGCGGGCGGCCCGGCUUCCCC 24 11654

HSV2-UL30-3301 - GAACGCAGCCAAAGGCCC 18 11655

HSV2-UL30-3302 - GGAACGCAGCCAAAGGCCC 19 11656

HSV2-UL30-262 - CGGAACGCAGCCAAAGGCCC 20 4667

HSV2-UL30-3303 - CCGGAACGCAGCCAAAGGCCC 21 11657

HSV2-UL30-3304 - ACCGGAACGCAGCCAAAGGCCC 22 11658

HSV2-UL30-3305 - GACCGGAACGCAGCCAAAGGCCC 23 11659

HSV2-UL30-3306 - AGACCGGAACGCAGCCAAAGGCCC 24 11660

HSV2-UL30-3307 - GCGGUGCGGACCAUGCCC 18 11661

HSV2-UL30-3308 - GGCGGUGCGGACCAUGCCC 19 11662

HSV2-UL30-286 - GGGCGGUGCGGACCAUGCCC 20 4682

HSV2-UL30-3309 - GGGGCGGUGCGGACCAUGCCC 21 11663

HSV2-UL30-3310 - UGGGGCGGUGCGGACCAUGCCC 22 11664

HSV2-UL30-3311 - GUGGGGCGGUGCGGACCAUGCCC 23 11665

HSV2-UL30-3312 - UGUGGGGCGGUGCGGACCAUGCCC 24 11666

HSV2-UL30-3313 - CGGGCGGCCCGGCUUCCC 18 11667

HSV2-UL30-3314 - GCGGGCGGCCCGGCUUCCC 19 11668

HSV2-UL30-247 - CGCGGGCGGCCCGGCUUCCC 20 4661

HSV2-UL30-3315 - CCGCGGGCGGCCCGGCUUCCC 21 11669

HSV2-UL30-3316 - GCCGCGGGCGGCCCGGCUUCCC 22 11670

HSV2-UL30-3317 - UGCCGCGGGCGGCCCGGCUUCCC 23 11671

HSV2-UL30-3318 - GUGCCGCGGGCGGCCCGGCUUCCC 24 11672

HSV2-UL30-3319 - ACGUCCUCCGCGUGGGCC 18 11673

HSV2-UL30-3320 - GACGUCCUCCGCGUGGGCC 19 11674

HSV2-UL30-41 - CGACGUCCUCCGCGUGGGCC 20 3186

HSV2-UL30-3321 - GCGACGUCCUCCGCGUGGGCC 21 11675

HSV2-UL30-3322 - CGCGACGUCCUCCGCGUGGGCC 22 11676

HSV2-UL30-3323 - GCGCGACGUCCUCCGCGUGGGCC 23 11677

HSV2-UL30-3324 - AGCGCGACGUCCUCCGCGUGGGCC 24 11678

HSV2-UL30-3325 - GCGGCGAUGUUUUGUGCC 18 11679

HSV2-UL30-3326 - GGCGGCGAUGUUUUGUGCC 19 11680

HSV2-UL30-3327 - CGGCGGCGAUGUUUUGUGCC 20 11681

HSV2-UL30-3328 - CCGGCGGCGAUGUUUUGUGCC 21 11682

HSV2-UL30-3329 - ACCGGCGGCGAUGUUUUGUGCC 22 11683

HSV2-UL30-3330 - GACCGGCGGCGAUGUUUUGUGCC 23 11684

HSV2-UL30-3331 - CGACCGGCGGCGAUGUUUUGUGCC 24 11685

HSV2-UL30-3332 - UCCACGUGUACGACAUCC 18 11686

HSV2-UL30-3333 - U U CCACG U G U ACG ACAU CC 19 11687

HSV2-UL30-53 - CUUCCACGUGUACGACAUCC 20 3084

HSV2-UL30-3334 - UCUUCCACGUGUACGACAUCC 21 11688

HSV2-UL30-3335 - GUCUUCCACGUGUACGACAUCC 22 11689 HSV2-UL30-3336 - CG UCU U CCACG U G U ACG ACAU CC 23 11690

HSV2-UL30-3337 - CCGUCUUCCACGUGUACGACAUCC 24 11691

HSV2-UL30-3338 - UGCGCGCCGCCCAGCUCC 18 11692

HSV2-UL30-3339 - AUGCGCGCCGCCCAGCUCC 19 11693

HSV2-UL30-3340 - CAUGCGCGCCGCCCAGCUCC 20 11694

HSV2-UL30-3341 - GCAUGCGCGCCGCCCAGCUCC 21 11695

HSV2-UL30-3342 - AGCAUGCGCGCCGCCCAGCUCC 22 11696

HSV2-UL30-3343 - CAGCAUGCGCGCCGCCCAGCUCC 23 11697

HSV2-UL30-3344 - ACAGCAUGCGCGCCGCCCAGCUCC 24 11698

HSV2-UL30-3345 - GAGCGCGACGUCCUCCGC 18 11699

HSV2-UL30-3346 - CGAGCGCGACGUCCUCCGC 19 11700

HSV2-UL30-279 - ACGAGCGCGACGUCCUCCGC 20 4677

HSV2-UL30-3347 - GACGAGCGCGACGUCCUCCGC 21 11701

HSV2-UL30-3348 - GGACGAGCGCGACGUCCUCCGC 22 11702

HSV2-UL30-3349 - GGGACGAGCGCGACGUCCUCCGC 23 11703

HSV2-UL30-3350 - GGGGACGAGCGCGACGUCCUCCGC 24 11704

HSV2-UL30-3351 - CGGGGGGAAGCCGGCGGC 18 11705

HSV2-UL30-3352 - CCGGGGGGAAGCCGGCGGC 19 11706

HSV2-UL30-253 - CCCGGGGGGAAGCCGGCGGC 20 4662

HSV2-UL30-3353 - CCCCGGGGGGAAGCCGGCGGC 21 11707

HSV2-UL30-3354 - CCCCCGGGGGGAAGCCGGCGGC 22 11708

HSV2-UL30-3355 - UCCCCCGGGGGGAAGCCGGCGGC 23 11709

HSV2-UL30-3356 - UUCCCCCGGGGGGAAGCCGGCGGC 24 11710

HSV2-UL30-3357 - GACGUCCUCCGCGUGGGC 18 11711

HSV2-UL30-3358 - CGACGUCCUCCGCGUGGGC 19 11712

HSV2-UL30-280 - GCGACGUCCUCCGCGUGGGC 20 4678

HSV2-UL30-3359 - CGCGACGUCCUCCGCGUGGGC 21 11713

HSV2-UL30-3360 - GCGCGACGUCCUCCGCGUGGGC 22 11714

HSV2-UL30-3361 - AGCGCGACGUCCUCCGCGUGGGC 23 11715

HSV2-UL30-3362 - GAGCGCGACGUCCUCCGCGUGGGC 24 11716

HSV2-UL30-3363 - GCCCCUAAGGUGUACUGC 18 11717

HSV2-UL30-3364 - CGCCCCUAAGGUGUACUGC 19 11718

HSV2-UL30-34 - GCGCCCCUAAGGUGUACUGC 20 3072

HSV2-UL30-3365 - CGCGCCCCUAAGGUGUACUGC 21 11719

HSV2-UL30-3366 - GCGCGCCCCUAAGGUGUACUGC 22 11720

HSV2-UL30-3367 - GGCGCGCCCCUAAGGUGUACUGC 23 11721

HSV2-UL30-3368 - CGGCGCGCCCCUAAGGUGUACUGC 24 11722

HSV2-UL30-3369 - UUCCACGUGUACGACAUC 18 11723

HSV2-UL30-3370 - CUUCCACGUGUACGACAUC 19 11724

HSV2-UL30-289 - UCUUCCACGUGUACGACAUC 20 4684

HSV2-UL30-3371 - GUCUUCCACGUGUACGACAUC 21 11725

HSV2-UL30-3372 - CGUCUUCCACGUGUACGACAUC 22 11726

HSV2-UL30-3373 - CCGUCUUCCACGUGUACGACAUC 23 11727

HSV2-UL30-3374 - ACCGUCUUCCACGUGUACGACAUC 24 11728

HSV2-UL30-3375 - ACGACAUCCUGGAGCACG 18 11729

HSV2-UL30-3376 - UACGACAUCCUGGAGCACG 19 11730 HSV2-UL30-54 - GUACGACAUCCUGGAGCACG 20 3192

HSV2-UL30-3377 - UGUACGACAUCCUGGAGCACG 21 11731

HSV2-UL30-3378 - GUGUACGACAUCCUGGAGCACG 22 11732

HSV2-UL30-3379 - CG U G U ACG ACAU CCU G G AG CACG 23 11733

HSV2-UL30-3380 - ACGUGUACGACAUCCUGGAGCACG 24 11734

HSV2-UL30-3381 - UGCCCCCCACAACCCCCG 18 11735

HSV2-UL30-3382 - UUGCCCCCCACAACCCCCG 19 11736

HSV2-UL30-16 - U U U GCCCCCCACAACCCCCG 20 3172

HSV2-UL30-3383 - U U U U GCCCCCCACAACCCCCG 21 11737

HSV2-UL30-3384 - U U U U U GCCCCCCACAACCCCCG 22 11738

HSV2-UL30-3385 - U U U U U U G CCCCCCACAACCCCCG 23 11739

HSV2-UL30-3386 - GU U U U U UGCCCCCCACAACCCCCG 24 11740

HSV2-UL30-3387 - UGGACGAGGACGCCCCCG 18 11741

HSV2-UL30-3388 - CUGGACGAGGACGCCCCCG 19 11742

HSV2-UL30-26 - GCUGGACGAGGACGCCCCCG 20 3179

HSV2-UL30-3389 - CGCUGGACGAGGACGCCCCCG 21 11743

HSV2-UL30-3390 - UCGCUGGACGAGGACGCCCCCG 22 11744

HSV2-UL30-3391 - UUCGCUGGACGAGGACGCCCCCG 23 11745

HSV2-UL30-3392 - GU U CGCU GGACGAGG ACGCCCCCG 24 11746

HSV2-UL30-3393 - GCGGCCCGGCUUCCCCCG 18 11747

HSV2-UL30-3394 - GGCGGCCCGGCUUCCCCCG 19 11748

HSV2-UL30-4 - GGGCGGCCCGGCUUCCCCCG 20 3160

HSV2-UL30-3395 - CGGGCGGCCCGGCUUCCCCCG 21 11749

HSV2-UL30-3396 - GCGGGCGGCCCGGCUUCCCCCG 22 11750

HSV2-UL30-3397 - CGCGGGCGGCCCGGCUUCCCCCG 23 11751

HSV2-UL30-3398 - CCGCGGGCGGCCCGGCUUCCCCCG 24 11752

HSV2-UL30-3399 - GUCCUCCGCGUGGGCCCG 18 11753

HSV2-UL30-3400 - CGUCCUCCGCGUGGGCCCG 19 11754

HSV2-UL30-282 - ACGUCCUCCGCGUGGGCCCG 20 4679

HSV2-UL30-3401 - GACGUCCUCCGCGUGGGCCCG 21 11755

HSV2-UL30-3402 - CGACGUCCUCCGCGUGGGCCCG 22 11756

HSV2-UL30-3403 - GCGACGUCCUCCGCGUGGGCCCG 23 11757

HSV2-UL30-3404 - CGCGACGUCCUCCGCGUGGGCCCG 24 11758

HSV2-UL30-3405 - CCCCUAAGGUGUACUGCG 18 11759

HSV2-UL30-3406 - GCCCCUAAGGUGUACUGCG 19 11760

HSV2-UL30-35 - CGCCCCUAAGGUGUACUGCG 20 3185

HSV2-UL30-3407 - GCGCCCCUAAGGUGUACUGCG 21 11761

HSV2-UL30-3408 - CGCGCCCCUAAGGUGUACUGCG 22 11762

HSV2-UL30-3409 - GCGCGCCCCUAAGGUGUACUGCG 23 11763

HSV2-UL30-3410 - GGCGCGCCCCUAAGGUGUACUGCG 24 11764

HSV2-UL30-3411 - UUUAUCGCCCCGCGUUCG 18 11765

HSV2-UL30-3412 - AUUUAUCGCCCCGCGUUCG 19 11766

HSV2-UL30-265 - GAUUUAUCGCCCCGCGUUCG 20 4670

HSV2-UL30-3413 - CGAUUUAUCGCCCCGCGUUCG 21 11767

HSV2-UL30-3414 - UCGAU UUAUCGCCCCGCGUUCG 22 11768

HSV2-UL30-3415 - UUCGAUUUAUCGCCCCGCGUUCG 23 11769 HSV2-UL30-3416 - UUUCGAUUUAUCGCCCCGCGUUCG 24 11770

HSV2-UL30-3417 - CGGCCCGGCUUCCCCCGG 18 11771

HSV2-UL30-3418 - GCGGCCCGGCUUCCCCCGG 19 11772

HSV2-UL30-5 - GGCGGCCCGGCUUCCCCCGG 20 3161

HSV2-UL30-3419 - GGGCGGCCCGGCUUCCCCCGG 21 11773

HSV2-UL30-3420 - CGGGCGGCCCGGCUUCCCCCGG 22 11774

HSV2-UL30-3421 - GCGGGCGGCCCGGCUUCCCCCGG 23 11775

HSV2-UL30-3422 - CGCGGGCGGCCCGGCUUCCCCCGG 24 11776

HSV2-UL30-3423 - CCGCCGCCUUGCCGCCGG 18 11777

HSV2-UL30-3424 - ACCGCCGCCUUGCCGCCGG 19 11778

HSV2-UL30-259 - CACCGCCGCCUUGCCGCCGG 20 4665

HSV2-UL30-3425 - GCACCGCCGCCUUGCCGCCGG 21 11779

HSV2-UL30-3426 - GGCACCGCCGCCUUGCCGCCGG 22 11780

HSV2-UL30-3427 - CGGCACCGCCGCCUUGCCGCCGG 23 11781

HSV2-UL30-3428 - ACGGCACCGCCGCCUUGCCGCCGG 24 11782

HSV2-UL30-3429 - CCCUAAGGUGUACUGCGG 18 11783

HSV2-UL30-3430 - CCCCUAAGGUGUACUGCGG 19 11784

HSV2-UL30-36 - GCCCCUAAGGUGUACUGCGG 20 3073

HSV2-UL30-3431 - CGCCCCUAAGGUGUACUGCGG 21 11785

HSV2-UL30-3432 - GCGCCCCUAAGGUGUACUGCGG 22 11786

HSV2-UL30-3433 - CGCGCCCCUAAGGUGUACUGCGG 23 11787

HSV2-UL30-3434 - GCGCGCCCCUAAGGUGUACUGCGG 24 11788

HSV2-UL30-3435 - GGCCCGGCUUCCCCCGGG 18 11789

HSV2-UL30-3436 - CGGCCCGGCUUCCCCCGGG 19 11790

HSV2-UL30-6 - GCGGCCCGGCUUCCCCCGGG 20 3162

HSV2-UL30-3437 - GGCGGCCCGGCUUCCCCCGGG 21 11791

HSV2-UL30-3438 - GGGCGGCCCGGCUUCCCCCGGG 22 11792

HSV2-UL30-3439 - CGGGCGGCCCGGCUUCCCCCGGG 23 11793

HSV2-UL30-3440 - GCGGGCGGCCCGGCUUCCCCCGGG 24 11794

HSV2-UL30-3441 - CCUAAGGUGUACUGCGGG 18 11795

HSV2-UL30-3442 - CCCUAAGGUGUACUGCGGG 19 11796

HSV2-UL30-37 - CCCCUAAGGUGUACUGCGGG 20 3074

HSV2-UL30-3443 - GCCCCUAAGGUGUACUGCGGG 21 11797

HSV2-UL30-3444 - CGCCCCUAAGGUGUACUGCGGG 22 11798

HSV2-UL30-3445 - GCGCCCCUAAGGUGUACUGCGGG 23 11799

HSV2-UL30-3446 - CGCGCCCCUAAGGUGUACUGCGGG 24 11800

HSV2-UL30-3447 - AGGUGUACUGCGGGGGGG 18 11801

HSV2-UL30-3448 - AAGGUGUACUGCGGGGGGG 19 11802

HSV2-UL30-278 - UAAGGUGUACUGCGGGGGGG 20 4676

HSV2-UL30-3449 - CU AAGGUGUACUGCGGGGGGG 21 11803

HSV2-UL30-3450 - CCUAAGGUGUACUGCGGGGGGG 22 11804

HSV2-UL30-3451 - CCCUAAGGUGUACUGCGGGGGGG 23 11805

HSV2-UL30-3452 - CCCCUAAGGUGUACUGCGGGGGGG 24 11806

HSV2-UL30-3453 - UCGCCCCGCGUUCGCUGG 18 11807

HSV2-UL30-3454 - AUCGCCCCGCGUUCGCUGG 19 11808

HSV2-UL30-266 - UAUCGCCCCGCGUUCGCUGG 20 4671 HSV2-UL30-3455 - UUAUCGCCCCGCGUUCGCUGG 21 11809

HSV2-UL30-3456 - UUUAUCGCCCCGCGUUCGCUGG 22 11810

HSV2-UL30-3457 - AUU UAUCGCCCCGCGUUCGCUGG 23 11811

HSV2-UL30-3458 - GAUUUAUCGCCCCGCGUUCGCUGG 24 11812

HSV2-UL30-3459 - CGCCCCUAAGGUGUACUG 18 11813

HSV2-UL30-3460 - GCGCCCCUAAGGUGUACUG 19 11814

HSV2-UL30-33 - CGCGCCCCUAAGGUGUACUG 20 3071

HSV2-UL30-3461 - GCGCGCCCCUAAGGUGUACUG 21 11815

HSV2-UL30-3462 - GGCGCGCCCCUAAGGUGUACUG 22 11816

HSV2-UL30-3463 - CGGCGCGCCCCUAAGGUGUACUG 23 11817

HSV2-UL30-3464 - CCGGCGCGCCCCUAAGGUGUACUG 24 11818

HSV2-UL30-3465 - CCGCGUCGCUUGCGCCUG 18 11819

HSV2-UL30-3466 - GCCGCGUCGCUUGCGCCUG 19 11820

HSV2-UL30-45 - GGCCGCGUCGCUUGCGCCUG 20 3189

HSV2-UL30-3467 - UGGCCGCGUCGCUUGCGCCUG 21 11821

HSV2-UL30-3468 - CUGGCCGCGUCGCUUGCGCCUG 22 11822

HSV2-UL30-3469 - UCUGGCCGCGUCGCUUGCGCCUG 23 11823

HSV2-UL30-3470 - UUCUGGCCGCGUCGCUUGCGCCUG 24 11824

HSV2-UL30-3471 - GCGCCCCUAAGGUGUACU 18 11825

HSV2-UL30-3472 - CGCGCCCCUAAGGUGUACU 19 11826

HSV2-UL30-272 - GCGCGCCCCUAAGGUGUACU 20 4675

HSV2-UL30-3473 - GGCGCGCCCCUAAGGUGUACU 21 11827

HSV2-UL30-3474 - CGGCGCGCCCCUAAGGUGUACU 22 11828

HSV2-UL30-3475 - CCGGCGCGCCCCUAAGGUGUACU 23 11829

HSV2-UL30-3476 - UCCGGCGCGCCCCUAAGGUGUACU 24 11830

HSV2-UL30-3477 - GCCGCGUCGCUUGCGCCU 18 11831

HSV2-UL30-3478 - GGCCGCGUCGCUUGCGCCU 19 11832

HSV2-UL30-283 - UGGCCGCGUCGCUUGCGCCU 20 4680

HSV2-UL30-3479 - CUGGCCGCGUCGCUUGCGCCU 21 11833

HSV2-UL30-3480 - UCUGGCCGCGUCGCUUGCGCCU 22 11834

HSV2-UL30-3481 - UUCUGGCCGCGUCGCUUGCGCCU 23 11835

HSV2-UL30-3482 - CUUCUGGCCGCGUCGCU UGCGCCU 24 11836

HSV2-UL30-3483 - UUGCGCCUGUGGGGCGGU 18 11837

HSV2-UL30-3484 - CUUGCGCCUGUGGGGCGGU 19 11838

HSV2-UL30-285 - GCUUGCGCCUGUGGGGCGGU 20 4681

HSV2-UL30-3485 - CGCUUGCGCCUGUGGGGCGGU 21 11839

HSV2-UL30-3486 - UCGCUUGCGCCUGUGGGGCGGU 22 11840

HSV2-UL30-3487 - GUCGCUUGCGCCUGUGGGGCGGU 23 11841

HSV2-UL30-3488 - CGUCGCUUGCGCCUGUGGGGCGGU 24 11842

HSV2-UL30-3489 - CAGCUCCACGAGCGAUUU 18 11843

HSV2-UL30-3490 - CCAGCUCCACGAGCGAU U U 19 11844

HSV2-UL30-3491 - CCCAGCUCCACGAGCGAU U U 20 11845

HSV2-UL30-3492 - GCCCAGCUCCACGAGCGAUUU 21 11846

HSV2-UL30-3493 - CGCCCAGCUCCACGAGCGAUUU 22 11847

HSV2-UL30-3494 - CCGCCCAGCUCCACGAGCGAUUU 23 11848

HSV2-UL30-3495 - GCCGCCCAGCUCCACGAGCGAUU U 24 11849 Table 9E provides exemplary targeting domains for knocking out the UL30 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene), and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 9E

HSV2-UL30-3517 + GGCGCAGGAGGCACGUGAAGAC 22 11871

HSV2-UL30-3518 + GUACCAGCCGAAGGUGACAAACC 23 11872

HSV2-UL30-3519 + GGUACCAGCCGAAGGUGACAAACC 24 11873

HSV2-UL30-1108 + GCCCCGGGCGAUGGCGUACC 20 3978

HSV2-UL30-3520 + GAACGCCCCGGGCGAUGGCGUACC 24 11874

HSV2-UL30-3521 + GAGGCCCCGAAACCGCCC 18 11875

HSV2-UL30-3522 + GUCGAGGCCCCGAAACCGCCC 21 11876

HSV2-UL30-3523 + GCU UGUUGGUGUACGCGC 18 11877

HSV2-UL30-2395 + GCGCU UGUUGGUGUACGCGC 20 5157

HSV2-UL30-3524 + GGCGCUUG UUGGUGUACGCGC 21 11878

HSV2-UL30-3525 + GCCCGGAGGCGUAGUAGGC 19 11879

HSV2-UL30-1318 + GGCCCGGAGGCGUAGUAGGC 20 4188

HSV2-UL30-3526 + GGGCCCGGAGGCGUAG UAGGC 21 11880

HSV2-UL30-3527 + GCGGGCCCGGAGGCGUAGUAGGC 23 11881

HSV2-UL30-3528 + GCCUGCAGUCCCUCGGGC 18 11882

HSV2-UL30-3529 + GAACGCCUGCAGUCCCUCGGGC 22 11883

HSV2-UL30-3530 + GAAGCACAGGUUGUGGGC 18 11884

HSV2-UL30-3531 + GUACUGAAGCACAGGUUGUGGGC 23 11885

HSV2-UL30-3532 + GCUUCGACGGGGGGCUCAUGC 21 11886

HSV2-UL30-1267 + GUUGUGGGCCUGGAUGAUGC 20 4137

HSV2-UL30-3533 + GGUUGUGGGCCUGGAUGAUGC 21 11887

HSV2-UL30-3534 + G U C A U G A AG G CC AG C AG C A U C 21 11888

HSV2-UL30-3535 + G G U C A U G A AG G CC AG C AG C A U C 22 11889

HSV2-UL30-3536 + GACCGUCUCCUCUACCUC 18 11890

HSV2-UL30-2385 + GCGACCGUCUCCUCUACCUC 20 5149

HSV2-UL30-3537 + GCGCGACCGUCUCCUCUACCUC 22 11891

HSV2-UL30-3538 + GAGGAGGACGGCCUCCUC 18 11892

HSV2-UL30-3539 + GUCGAGGAGGACGGCCUCCUC 21 11893

HSV2-UL30-3540 + GUCCGGGGGGUGCCACGUCUC 21 11894

HSV2-UL30-3541 + GUCGUCCGGGGGGUGCCACGUCUC 24 11895

HSV2-UL30-3542 + GGCGGUGAGGACAAAGUC 18 11896

HSV2-UL30-3543 + GUUCGGCGG UGAGGACAAAGUC 22 11897

HSV2-UL30-3544 + G ACAAACCCCGGG U U G U C 18 11898

HSV2-UL30-2571 + GUGACAAACCCCGGGUUGUC 20 5284

HSV2-UL30-3545 + GGUG ACAAACCCCGGG UUGUC 21 11899

HSV2-UL30-3546 + GAAGGUGACAAACCCCGGGUUGUC 24 11900

HSV2-UL30-3547 + GGCGACGCGAUGGCCU UC 18 11901

HSV2-UL30-3548 + GAACGGCGACGCGAUGGCCUUC 22 11902

HSV2-UL30-3549 + GUGAACGGCGACGCGAUGGCCUUC 24 11903

HSV2-UL30-3550 + GGAUCCGAGCGAAAACAG 18 11904

HSV2-UL30-3551 + GCAGGAUCCGAGCGAAAACAG 21 11905

HSV2-UL30-3552 + GUCGCAGGAUCCGAGCGAAAACAG 24 11906

HSV2-UL30-3553 + GGCCAGCCAG UCGCGCAG 18 11907

HSV2-UL30-3554 + GCAUGGCCAGCCAGUCGCGCAG 22 11908

HSV2-UL30-3555 + GCUUGGACGCGCCUCCCG 18 11909

HSV2-UL30-3556 + GGCUUGGACGCGCCUCCCG 19 11910 HSV2-UL30-1117 + GGGCU UGGACGCGCCUCCCG 20 3987

HSV2-UL30-3557 + GGGGCU UGGACGCGCCUCCCG 21 11911

HSV2-UL30-3558 + GCGGGGCUUGGACGCGCCUCCCG 23 11912

HSV2-UL30-3559 + GCGCGGCCACGUCGUCCG 18 11913

HSV2-UL30-3560 + GCCGCGCGGCCACGUCGUCCG 21 11914

HSV2-UL30-3561 + GAGCCGCGCGGCCACGUCGUCCG 23 11915

HSV2-UL30-3562 + GUGGGACUCGGGGAGGUCG 19 11916

HSV2-UL30-2541 + GGUGGGACUCGGGGAGGUCG 20 5260

HSV2-UL30-3563 + GAGGUGGGACUCGGGGAGGUCG 22 11917

HSV2-UL30-3564 + GGCUCUAUGCAACAUUCG 18 11918

HSV2-UL30-1277 + GUCGACGUGAAACCCGGAGG 20 4147

HSV2-UL30-3565 + G G U CG ACG U G AAACCCG G AG G 21 11919

HSV2-UL30-3566 + G G G U CG ACG U G AAACCCG GAG G 22 11920

HSV2-UL30-3567 + GGGGUCGACGUGAAACCCGGAGG 23 11921

HSV2-UL30-3568 + GAAACCGCCCCUGGGUG UCCGG 22 11922

HSV2-UL30-3569 + GCGU UCAGCUUGUAGCUG 18 11923

HSV2-UL30-3570 + GGCGUUCAGCUUGUAGCUG 19 11924

HSV2-UL30-3571 + GACGGCGUUCAGCUUGUAGCUG 22 11925

HSV2-UL30-3572 + GCGACGGCGU UCAGCU UGUAGCUG 24 11926

HSV2-UL30-1246 + GCCUCCUCGGGGGUGCUCUG 20 4116

HSV2-UL30-3573 + GGCCUCCUCGGGGGUGCUCUG 21 11927

HSV2-UL30-3574 + GACGGCCUCCUCGGGGGUGCUCUG 24 11928

HSV2-UL30-3575 + GUAGAGCAGACAGGAGAU 18 11929

HSV2-UL30-3576 + GUCGUAGAGCAGACAGGAGAU 21 11930

HSV2-UL30-3577 + GGUCGUAGAGCAGACAGGAGAU 22 11931

HSV2-UL30-3578 + GCUAACGCGGCGGCCGCU 18 11932

HSV2-UL30-3579 + GGCUAACGCGGCGGCCGCU 19 11933

HSV2-UL30-3580 + GCUCGGCUAACGCGGCGGCCGCU 23 11934

HSV2-UL30-3581 + GACAAAGUCCUGGAUGUCCCUCU 23 11935

HSV2-UL30-3582 + GGACAAAGUCCUGGAUGUCCCUCU 24 11936

HSV2-UL30-3583 + GCUGGCCGUCGUAGAUGGU 19 11937

HSV2-UL30-3584 + GAUCUGCUGGCCGUCGUAGAUGGU 24 11938

HSV2-UL30-3585 - GCAGCAAGAUCAAGGUGAA 19 11939

HSV2-UL30-3586 - G CG C AG C A AG A U C A AG G U G A A 21 11940

HSV2-UL30-3587 - GAAGCGCAGCAAGAUCAAGGUGAA 24 11941

HSV2-UL30-3588 - GUCGUCCUGGAGUUUGACA 19 11942

HSV2-UL30-3589 - GGUCCCGUCCAUCAAGGA 18 11943

HSV2-UL30-3590 - GCAGGUCCCGUCCAUCAAGGA 21 11944

HSV2-UL30-3591 - GCGCAGGUCCCGUCCAU C A AG G A 23 11945

HSV2-UL30-1859 - GUACGGCCCCGAGU UCGUGA 20 4811

HSV2-UL30-3592 - GCAGUACGGCCCCGAGU UCGUGA 23 11946

HSV2-UL30-3593 - GCAACUCGGUG UACGGG UUCAC 22 11947

HSV2-UL30-3594 - GUGCAACUCGG UGUACGGGUUCAC 24 11948

HSV2-UL30-3595 - GGGGACGGGAACGGGGAC 18 11949

HSV2-UL30-3596 - GGGGGACGGGAACGGGGAC 19 11950

HSV2-UL30-3597 - GCCGGGGGACGGGAACGGGGAC 22 11951 HSV2-UL30-3598 - GGCCGGGGGACGGGAACGGGGAC 23 11952

HSV2-UL30-3599 - GGUUUAUCCUGGACAACC 18 11953

HSV2-UL30-2047 - GAAAUAACGCCAAGAUCACC 20 4934

HSV2-UL30-3600 - GGAAAUAACG CC AAG A U CACC 21 11954

HSV2-UL30-3601 - GACCUUCGUCAAGCAGUACGGCC 23 11955

HSV2-UL30-1851 - GCUCGGAUCCUGCGACCUCC 20 4805

HSV2-UL30-3602 - GCCUGCCGGCCCCCGUCGUCC 21 11956

HSV2-UL30-3603 - G G CCU GCCG G CCCCCG U CG U CC 22 11957

HSV2-UL30-3604 - GGGCCUGCCGGCCCCCGUCGUCC 23 11958

HSV2-UL30-3605 - GGGGCCUGCCGGCCCCCGUCGUCC 24 11959

HSV2-UL30-1988 - GUUCCUCCCCCCGAUCAAGC 20 4895

HSV2-UL30-3606 - GCUGUUCCUCCCCCCGAUCAAGC 23 11960

HSV2-UL30-1850 - GCACAUCCUCCUGUU UUCGC 20 4804

HSV2-UL30-3607 - GAGCACAUCCUCCUGU UU UCGC 22 11961

HSV2-UL30-3608 - GUACGGGCGCAUGAACGGC 19 11962

HSV2-UL30-529 - GGUACGGGCGCAUGAACGGC 20 3399

HSV2-UL30-3609 - GGGUACGGGCGCAUGAACGGC 21 11963

HSV2-UL30-3610 - GACGGGUACGGGCGCAUGAACGGC 24 11964

HSV2-UL30-1995 - GCAAGAUGCUCAUCAAGGGC 20 4900

HSV2-UL30-3611 - GGCAAGAUGCUCAUCAAGGGC 21 11965

HSV2-UL30-3612 - GGGCAAGAUGCUCAUCAAGGGC 22 11966

HSV2-UL30-3613 - GGGGCAAGAUGCUCAUCAAGGGC 23 11967

HSV2-UL30-3614 - GGGGGCAAGAUGCUCAUCAAGGGC 24 11968

HSV2-UL30-3615 - GCACGUUGGGUACCAGGGGGC 21 11969

HSV2-UL30-3616 - GGCACGUUGGGUACCAGGGGGC 22 11970

HSV2-UL30-3617 - GCCUGGCGGCGGCCCUGC 18 11971

HSV2-UL30-1800 - GCGCCUGGCGGCGGCCCUGC 20 4776

HSV2-UL30-3618 - GAGCGCCUGGCGGCGGCCCUGC 22 11972

HSV2-UL30-3619 - GCGAGCGCCUGGCGGCGGCCCUGC 24 11973

HSV2-UL30-2037 - GUCCGAGCUGGCGGAGGAUC 20 4928

HSV2-UL30-3620 - GUGUCCGAGCUGGCGGAGGAUC 22 11974

HSV2-UL30-3621 - GGUGUCCGAGCUGGCGGAGGAUC 23 11975

HSV2-UL30-3622 - GCGAAAGCAGAUCCGCUC 18 11976

HSV2-UL30-3623 - GCCAUGCGAAAGCAGAUCCGCUC 23 11977

HSV2-UL30-3624 - GGCCAUGCGAAAGCAGAUCCGCUC 24 11978

HSV2-UL30-3625 - G U C U U G A AG G AC A AG A AG 18 11979

HSV2-UL30-3626 - GCCGUCU UGAAGGACAAGAAG 21 11980

HSV2-UL30-3627 - GGCCGUCUUGAAGGACAAGAAG 22 11981

HSV2-UL30-3628 - GAGGCCGUCUUGAAGGACAAGAAG 24 11982

HSV2-UL30-691 - GUUAGCCGAGCGCCCCGCAG 20 3561

HSV2-UL30-3629 - GCGUUAGCCGAGCGCCCCGCAG 22 11983

HSV2-UL30-3630 - GAGACCGGGGGCCGGCACG 19 11984

HSV2-UL30-3631 - GCGAGACCGGGGGCCGGCACG 21 11985

HSV2-UL30-3632 - GCGCGAGACCGGGGGCCGGCACG 23 11986

HSV2-UL30-1829 - GUUCGGAACCUCGAGCGACG 20 4793

HSV2-UL30-3633 - GCGUUCGGAACCUCGAGCGACG 22 11987 HSV2-UL30-3634 - GGCGUUCGGAACCUCGAGCGACG 23 11988

HSV2-UL30-3635 - GCCGCGCGGCUCAGGGCCG 19 11989

HSV2-UL30-751 - GGCCGCGCGGCUCAGGGCCG 20 3621

HSV2-UL30-3636 - GUGGCCGCGCGGCUCAGGGCCG 22 11990

HSV2-UL30-546 - GCCUCCGGGCCCGCGCAGCG 20 3416

HSV2-UL30-3637 - GCUGGUGUCCGAGCUGGCG 19 11991

HSV2-UL30-3638 - GCUGCUGGUGUCCGAGCUGGCG 22 11992

HSV2-UL30-3639 - GCGCAGCGCGGGGUGAUCG 19 11993

HSV2-UL30-3640 - GCCCGCGCAGCGCGGGGUGAUCG 23 11994

HSV2-UL30-3641 - GGCCCGCGCAGCGCGGGGUGAUCG 24 11995

HSV2-UL30-2062 - GCUACGGCGGAGGAAACUCG 20 4944

HSV2-UL30-3642 - GCGCUACGGCGGAGGAAACUCG 22 11996

HSV2-UL30-3643 - GGCGCUACGGCGGAGGAAACUCG 23 11997

HSV2-UL30-1862 - GAUCUACAAGGUCCCGCUCG 20 4813

HSV2-UL30-3644 - GAGAUCUACAAGGUCCCGCUCG 22 11998

HSV2-UL30-3645 - GGAGAUCUACAAGGUCCCGCUCG 23 11999

HSV2-UL30-657 - GUACGUGCACGCGCGCUGGG 20 3527

HSV2-UL30-3646 - GCGUACGUGCACGCGCGCUGGG 22 12000

HSV2-UL30-3647 - GCGCGUACGUGCACGCGCGCUGGG 24 12001

HSV2-UL30-3648 - GGGUCCUCGACCCCACCU 18 12002

HSV2-UL30-3649 - GCCCGGGUCCUCGACCCCACCU 22 12003

HSV2-UL30-3650 - GGCCCGGGUCCUCGACCCCACCU 23 12004

HSV2-UL30-3651 - GGGCCCGGGUCCUCGACCCCACCU 24 12005

HSV2-UL30-3652 - GUGGUGUGCAACUCGGUGU 19 12006

HSV2-UL30-1972 - GGUGGUGUGCAACUCGGUGU 20 4884

HSV2-UL30-3653 - G GGACCG U C AU CACG CU U 18 12007

HSV2-UL30-3654 - GCCGGGACCGUCAUCACGCU U 21 12008

Table 9F provides exemplary targeting domains for knocking out the UL30 gene selected according to the six tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 9F

HSV2-UL30-3656 + CCGCGGCACAAAACGAAAA 19 12010

HSV2-UL30-3657 + AGGCCGCGG C AC A A A ACG A A A A 22 12011

HSV2-UL30-3658 + UGAGGCCGCGGCACAAAACGAAAA 24 12012

HSV2-UL30-3659 + CGGGUUGUCCAGGAUAAA 18 12013

HSV2-UL30-3660 + CCGGGUUGUCCAGGAUAAA 19 12014

HSV2-UL30-2572 + CCCGGGU UGUCCAGGAUAAA 20 5285

HSV2-UL30-3661 + CCCCGGGUUGUCCAGGAUAAA 21 12015

HSV2-UL30-3662 + ACCCCGGGU UGUCCAGGAUAAA 22 12016

HSV2-UL30-3663 + AACCCCGGGU UGUCCAGGAUAAA 23 12017

HSV2-UL30-3664 + AAACCCCGGGUUGUCCAGGAUAAA 24 12018

HSV2-UL30-3665 + CCCCGG U G AACCCG U ACA 18 12019

HSV2-UL30-3666 + ACCCCGG U G AACCCG U ACA 19 12020

HSV2-UL30-2445 + CACCCCGGUGAACCCGUACA 20 5192

HSV2-UL30-3667 + UGCACCCCGGUGAACCCGUACA 22 12021

HSV2-UL30-3668 + CUGCACCCCGGUG AACCCG U ACA 23 12022

HSV2-UL30-3669 + C A A AG U C A A AC ACC ACC A 18 12023

HSV2-UL30-3670 + U G GC AA AG U CA AACACCACCA 21 12024

HSV2-UL30-3671 + C U G G C A A AG U C A A AC ACC ACC A 22 12025

HSV2-UL30-3672 + ACAGCUGCCCGACCAGCA 18 12026

HSV2-UL30-3673 + AACAGCUGCCCGACCAGCA 19 12027

HSV2-UL30-3674 + AGAACAGCUGCCCGACCAGCA 21 12028

HSV2-UL30-3675 + AAG AACAGCUGCCCGACCAGCA 22 12029

HSV2-UL30-3676 + UGAAG AACAGCUGCCCGACCAGCA 24 12030

HSV2-UL30-3677 + CCCCGUAGAUGAUGCGCA 18 12031

HSV2-UL30-3678 + UCCCCGUAGAUGAUGCGCA 19 12032

HSV2-UL30-3679 + UGUCCCCGUAGAUGAUGCGCA 21 12033

HSV2-UL30-3680 + CGUGUCCCCG UAGAUGAUGCGCA 23 12034

HSV2-UL30-3681 + CCGUGUCCCCGUAGAUGAUGCGCA 24 12035

HSV2-UL30-3682 + UGCUCUGGGGGAUCCGCGA 19 12036

HSV2-UL30-3683 + CACGAAGACGCGGUAG UA 18 12037

HSV2-UL30-2576 + CGCACGAAGACGCGGUAGUA 20 5288

HSV2-UL30-3684 + UCGCACGAAGACGCGGUAGUA 21 12038

HSV2-UL30-3685 + UUCGCACGAAGACGCGGUAGUA 22 12039

HSV2-UL30-3686 + CU UCGCACGAAGACGCGGUAGUA 23 12040

HSV2-UL30-3687 + CUGGGCCACGAUCACGUA 18 12041

HSV2-UL30-3688 + UCUGGGCCACGAUCACGUA 19 12042

HSV2-UL30-3689 + CGGGUCUGGGCCACGAUCACGUA 23 12043

HSV2-UL30-3690 + CAGGAGGCACGUGAAGAC 18 12044

HSV2-UL30-2494 + CGCAGGAGGCACG UGAAG AC 20 5228

HSV2-UL30-3691 + AGGCGCAGGAGGCACG UGAAG AC 23 12045

HSV2-UL30-3692 + AAGGCGCAGGAGGCACGUGAAGAC 24 12046

HSV2-UL30-3693 + AGCCGAAGGUGACAAACC 18 12047

HSV2-UL30-3694 + CAGCCGAAGGUGACAAACC 19 12048

HSV2-UL30-2570 + CCAGCCGAAGGUGACAAACC 20 5283

HSV2-UL30-3695 + ACC AG CCG A AG G U G AC A A ACC 21 12049

HSV2-UL30-3696 + UACCAGCCGAAGGUG AC A A ACC 22 12050 HSV2-UL30-3697 + CCCGGGCGAUGGCGUACC 18 12051

HSV2-UL30-3698 + CCCCGGGCGAUGGCGUACC 19 12052

HSV2-UL30-3699 + CGCCCCGGGCGAUGGCGUACC 21 12053

HSV2-UL30-3700 + ACGCCCCGGGCGAUGGCGUACC 22 12054

HSV2-UL30-3701 + AACGCCCCGGGCGAUGGCGUACC 23 12055

HSV2-UL30-3702 + CG AGG CCCCG AAACCG CCC 19 12056

HSV2-UL30-2488 + UCGAGGCCCCGAAACCGCCC 20 5223

HSV2-UL30-3703 + UGUCGAGGCCCCGAAACCGCCC 22 12057

HSV2-UL30-3704 + UUG UCGAGGCCCCGAAACCGCCC 23 12058

HSV2-UL30-3705 + CUUGUCGAGGCCCCGAAACCGCCC 24 12059

HSV2-UL30-3706 + CGCU UGU UGGUGUACGCGC 19 12060

HSV2-UL30-3707 + AGGCGCU UGUUGGUGUACGCGC 22 12061

HSV2-UL30-3708 + CAGGCGCU UGUUGGUGUACGCGC 23 12062

HSV2-UL30-3709 + CCAGGCGCUUGUUGGUGUACGCGC 24 12063

HSV2-UL30-3710 + CCCGGAGGCGUAGUAGGC 18 12064

HSV2-UL30-3711 + CGGGCCCGGAGGCGUAGUAGGC 22 12065

HSV2-UL30-3712 + CGCGGGCCCGGAGGCGUAGUAGGC 24 12066

HSV2-UL30-3713 + CGCCUGCAGUCCCUCGGGC 19 12067

HSV2-UL30-1184 + ACGCCUGCAGUCCCUCGGGC 20 4054

HSV2-UL30-3714 + AACGCCUGCAGUCCCUCGGGC 21 12068

HSV2-UL30-3715 + CGAACGCCUGCAGUCCCUCGGGC 23 12069

HSV2-UL30-3716 + CCGAACGCCUGCAGUCCCUCGGGC 24 12070

HSV2-UL30-3717 + UGAAGCACAGGUUGUGGGC 19 12071

HSV2-UL30-2470 + CUGAAGCACAGGUUGUGGGC 20 5209

HSV2-UL30-3718 + ACUGAAGCACAGGUUGUGGGC 21 12072

HSV2-UL30-3719 + UACUGAAGCACAGGUUGUGGGC 22 12073

HSV2-UL30-3720 + CGUACUGAAGCACAGGU UGUGGGC 24 12074

HSV2-UL30-3721 + UCGACGGGGGGCUCAUGC 18 12075

HSV2-UL30-3722 + U UCGACGGGGGGCUCAUGC 19 12076

HSV2-UL30-3723 + CUUCGACGGGGGGCUCAUGC 20 12077

HSV2-UL30-3724 + AGCU UCGACGGGGGGCUCAUGC 22 12078

HSV2-UL30-3725 + CAGCU UCGACGGGGGGCUCAUGC 23 12079

HSV2-UL30-3726 + UCAGCUUCGACGGGGGGCUCAUGC 24 12080

HSV2-UL30-3727 + UGUGGGCCUGGAUGAUGC 18 12081

HSV2-UL30-3728 + U UGUGGGCCUGGAUGAUGC 19 12082

HSV2-UL30-3729 + AGGU UGUGGGCCUGGAUGAUGC 22 12083

HSV2-UL30-3730 + CAGG UUGUGGGCCUGGAUGAUGC 23 12084

HSV2-UL30-3731 + ACAGGU UGUGGGCCUGGAUGAUGC 24

12085

HSV2-UL30-3732 + A U G A AG G CC AG C AG C A U C 18 12086

HSV2-UL30-3733 + C A U G A AG G CC AG C AG C A U C 19 12087

HSV2-UL30-2526 + UCAUGAAGGCCAGCAGCAUC 20 5252

HSV2-UL30-3734 + AGGUCAUGAAGGCCAGCAGCAUC 23 12088

HSV2-UL30-3735 + AAGG UCAUGAAGGCCAGCAGCAUC 24 12089

HSV2-UL30-3736 + CGACCGUCUCCUCUACCUC 19 12090

HSV2-UL30-3737 + CGCGACCGUCUCCUCUACCUC 21 12091 HSV2-UL30-3738 + CGCGCGACCGUCUCCUCUACCUC 23 12092

HSV2-UL30-3739 + CCGCGCGACCGUCUCCUCUACCUC 24 12093

HSV2-UL30-3740 + CGAGGAGGACGGCCUCCUC 19 12094

HSV2-UL30-1241 + UCGAGGAGGACGGCCUCCUC 20 4111

HSV2-UL30-3741 + UGUCGAGGAGGACGGCCUCCUC 22 12095

HSV2-UL30-3742 + UUGUCGAGGAGGACGGCCUCCUC 23 12096

HSV2-UL30-3743 + CUUGUCGAGGAGGACGGCCUCCUC 24 12097

HSV2-UL30-3744 + CGGGGGGUGCCACGUCUC 18 12098

HSV2-UL30-3745 + CCGGGGGGUGCCACGUCUC 19 12099

HSV2-UL30-1097 + UCCGGGGGG UGCCACGUCUC 20 3967

HSV2-UL30-3746 + CGUCCGGGGGGUGCCACGUCUC 22 12100

HSV2-UL30-3747 + UCGUCCGGGGGGUGCCACGUCUC 23 12101

HSV2-UL30-3748 + CGGCGGUGAGGACAAAGUC 19 12102

HSV2-UL30-2398 + UCGGCGGUGAGGACAAAGUC 20 5160

HSV2-UL30-3749 + U UCGGCGGUGAGGACAAAGUC 21 12103

HSV2-UL30-3750 + AGUUCGGCGGUG AG G AC A A AG U C 23 12104

HSV2-UL30-3751 + CAGU UCGGCGGUGAGGACAAAGUC 24 12105

HSV2-UL30-3752 + U G AC AAACCCCG G G U U G U C 19 12106

HSV2-UL30-3753 + AG G U G AC AAACCCCG G G U U G U C 22 12107

HSV2-UL30-3754 + A AG G U G AC AAACCCCG G G U U G U C 23 12108

HSV2-UL30-3755 + CGGCGACGCGAUGGCCUUC 19 12109

HSV2-UL30-1430 + ACGGCGACGCGAUGGCCUUC 20 4300

HSV2-UL30-3756 + AACGGCGACGCGAUGGCCUUC 21 12110

HSV2-UL30-3757 + UGAACGGCGACGCGAUGGCCUUC 23 12111

HSV2-UL30-3758 + AGGAUCCGAGCGAAAACAG 19 12112

HSV2-UL30-2546 + CAGGAUCCGAGCGAAAACAG 20 5264

HSV2-UL30-3759 + CGCAGGAUCCGAGCGAAAACAG 22 12113

HSV2-UL30-3760 + UCGCAGGAUCCGAGCG A A A AC AG 23 12114

HSV2-UL30-3761 + UGGCCAGCCAGUCGCGCAG 19 12115

HSV2-UL30-2459 + AUGGCCAGCCAGUCGCGCAG 20 5200

HSV2-UL30-3762 + CAUGGCCAGCCAGUCGCGCAG 21 12116

HSV2-UL30-3763 + CGCAUGGCCAGCCAGUCGCGCAG 23 12117

HSV2-UL30-3764 + UCGCAUGGCCAGCCAGUCGCGCAG 24 12118

HSV2-UL30-3765 + CGGGGCUUGGACGCGCCUCCCG 22 12119

HSV2-UL30-3766 + UGCGGGGCUUGGACGCGCCUCCCG 24 12120

HSV2-UL30-3767 + CGCGCGGCCACGUCGUCCG 19 12121

HSV2-UL30-1093 + CCGCGCGGCCACGUCGUCCG 20 3963

HSV2-UL30-3768 + AGCCGCGCGGCCACGUCGUCCG 22 12122

HSV2-UL30-3769 + UGAGCCGCGCGGCCACGUCGUCCG 24 12123

HSV2-UL30-3770 + UGGGACUCGGGGAGGUCG 18 12124

HSV2-UL30-3771 + AGGUGGGACUCGGGGAGGUCG 21 12125

HSV2-UL30-3772 + UGAGG UGGGACUCGGGGAGGUCG 23 12126

HSV2-UL30-3773 + CUGAGGUGGGACUCGGGGAGGUCG 24

12127

HSV2-UL30-3774 + AGGCUCUAUGCAACAUUCG 19 12128

HSV2-UL30-2337 + AAGGCUCU AUGCAACAU UCG 20 5123 HSV2-UL30-3775 + AAAGGCUCUAUGCAACAUUCG 21 12129

HSV2-UL30-3776 + CAAAGGCUCUAUGCAACAUUCG 22 12130

HSV2-UL30-3777 + UCAAAGGCUCUAUGCAACAU UCG 23 12131

HSV2-UL30-3778 + AUCAAAGGCUCUAUGCAACAUUCG 24 12132

HSV2-UL30-3779 + CGACGUGAAACCCGGAGG 18 12133

HSV2-UL30-3780 + UCGACGUGAAACCCGGAGG 19 12134

HSV2-UL30-3781 + CGGGGUCGACGUGAAACCCGGAGG 24 12135

HSV2-UL30-3782 + CCGCCCCUGGGUGUCCGG 18 12136

HSV2-UL30-3783 + ACCGCCCCUGGGUGUCCGG 19 12137

HSV2-UL30-2489 + AACCGCCCCUGGGUG UCCGG 20 5224

HSV2-UL30-3784 + A A ACCG CCCCUGGGUGUCCGG 21 12138

HSV2-UL30-3785 + CGAAACCGCCCCUGGGUGUCCGG 23 12139

HSV2-UL30-3786 + CCGAAACCGCCCCUGGGUGUCCGG 24 12140

HSV2-UL30-2510 + CGGCGUUCAGCUUGUAGCUG 20 5239

HSV2-UL30-3787 + ACGGCGU UCAGCU UGUAGCUG 21 12141

HSV2-UL30-3788 + CGACGGCGU UCAGCUUG UAGCUG 23 12142

HSV2-UL30-3789 + CUCCUCGGGGGUGCUCUG 18 12143

HSV2-UL30-3790 + CCUCCUCGGGGG UGCUCUG 19 12144

HSV2-UL30-3791 + CGGCCUCCUCGGGGGUGCUCUG 22 12145

HSV2-UL30-3792 + ACGGCCUCCUCGGGGGUGCUCUG 23 12146

HSV2-UL30-3793 + CGUAGAGCAGACAGGAGAU 19 12147

HSV2-UL30-2553 + UCGUAGAGCAGACAGGAGAU 20 5270

HSV2-UL30-3794 + AGGUCGUAGAGCAGACAGGAGAU 23 12148

HSV2-UL30-3795 + CAGGUCGUAGAGCAGACAGGAGAU 24 12149

HSV2-UL30-2409 + CGGCUAACGCGGCGGCCGCU 20 5169

HSV2-UL30-3796 + UCGGCUAACGCGGCGGCCGCU 21 12150

HSV2-UL30-3797 + CUCGGCUAACGCGGCGGCCGCU 22 12151

HSV2-UL30-3798 + CGCUCGGCUAACGCGGCGGCCGCU 24 12152

HSV2-UL30-3799 + AGUCCUGGAUGUCCCUCU 18 12153

HSV2-UL30-3800 + AAGUCCUGGAUGUCCCUCU 19 12154

HSV2-UL30-2399 + AAAGUCCUGGAUGUCCCUCU 20 5161

HSV2-UL30-3801 + CAAAGUCCUGGAUGUCCCUCU 21 12155

HSV2-UL30-3802 + ACAAAGUCCUGGAUGUCCCUCU 22 12156

HSV2-UL30-3803 + CUGGCCGUCGUAGAUGGU 18 12157

HSV2-UL30-2495 + UGCUGGCCGUCGUAGAUGGU 20 5229

HSV2-UL30-3804 + CUGCUGGCCGUCGUAGAUGGU 21 12158

HSV2-UL30-3805 + UCUGCUGGCCGUCGUAGAUGGU 22 12159

HSV2-UL30-3806 + AUCUGCUGGCCGUCGUAGAUGGU 23 12160

HSV2-UL30-3807 - CAG C A AG AUCAAGGUGAA 18 12161

HSV2-UL30-536 - CGCAGCAAGAUCAAGGUGAA 20 3406

HSV2-UL30-3808 - AGCGCAGCAAG AUCAAGGUGAA 22 12162

HSV2-UL30-3809 - AAGCGCAGCAAG AUCAAGGUGAA 23 12163

HSV2-UL30-3810 - UCGUCCUGGAGUUUGACA 18 12164

HSV2-UL30-1856 - CGUCGUCCUGGAGUU UGACA 20 4808

HSV2-UL30-3811 - CCGUCGUCCUGGAGUUUGACA 21 12165

HSV2-UL30-3812 - CCCGUCGUCCUGGAGU UUGACA 22 12166 HSV2-UL30-3813 - CCCCGUCGUCCUGGAGUUUGACA 23 12167

HSV2-UL30-3814 - CCCCCGUCGUCCUGGAGUU UGACA 24 12168

HSV2-UL30-3815 - AGGUCCCGUCCAUCAAGGA 19 12169

HSV2-UL30-2017 - CAGG UCCCGUCCAUCAAGGA 20 4916

HSV2-UL30-3816 - CGCAGGUCCCGUCCAUCAAGGA 22 12170

HSV2-UL30-3817 - CGCGCAGGUCCCGUCCAU C A AG G A 24 12171

HSV2-UL30-3818 - ACGGCCCCGAGU UCGUGA 18 12172

HSV2-UL30-3819 - UACGGCCCCGAGUUCGUGA 19 12173

HSV2-UL30-3820 - AGU ACGGCCCCGAGU UCGUGA 21 12174

HSV2-UL30-3821 - C AG UACGGCCCCGAGUUCGUGA 22 12175

HSV2-UL30-3822 - AGCAGUACGGCCCCGAGUUCGUGA 24 12176

HSV2-UL30-3823 - AGCUCAUGUGCUUCGAUA 18 12177

HSV2-UL30-3824 - AAGCUCAUGUGCU UCGAUA 19 12178

HSV2-UL30-1835 - CAAGCUCAUGUGCUUCGAUA 20 4797

HSV2-UL30-3825 - ACAAGCUCAUGUGCUUCGAUA 21 12179

HSV2-UL30-3826 - UACAAGCUCAUGUGCUUCGAUA 22 12180

HSV2-UL30-3827 - CUACAAGCUCAUGUGCU UCGAUA 23 12181

HSV2-UL30-3828 - CCUACAAGCUCAUGUGCUUCGAUA 24 12182

HSV2-UL30-3829 - CUCGGUGUACGGGU UCAC 18 12183

HSV2-UL30-3830 - ACUCGGUGUACGGGUUCAC 19 12184

HSV2-UL30-649 - AACUCGGUGUACGGGUUCAC 20 3519

HSV2-UL30-3831 - CAACUCGGUGUACGGGU UCAC 21 12185

HSV2-UL30-3832 - UGCAACUCGGUGUACGGGUUCAC 23 12186

HSV2-UL30-1923 - CGGGGGACGGGAACGGGGAC 20 4851

HSV2-UL30-3833 - CCGGGGGACGGGAACGGGGAC 21 12187

HSV2-UL30-3834 - CGGCCGGGGGACGGGAACGGGGAC 24 12188

HSV2-UL30-3835 - CGGUU UAUCCUGGACAACC 19 12189

HSV2-UL30-480 - CCGGU UUAUCCUGGACAACC 20 3350

HSV2-UL30-3836 - CCCG GUU UAUCCU GGACAACC 21 12190

HSV2-UL30-3837 - ACCCGGU U U AUCCUGG ACAACC 22 12191

HSV2-UL30-3838 - CACCCGGU UUAUCCUGGACAACC 23 12192

HSV2-UL30-3839 - CCACCCGGUUUAUCCUGGACAACC 24 12193

HSV2-UL30-3840 - AAUAACGCCAAGAUCACC 18 12194

HSV2-UL30-3841 - AAAUAACGCCAAGAUCACC 19 12195

HSV2-UL30-3842 - UGGAAAUAACGCCAAGAUCACC 22 12196

HSV2-UL30-3843 - UUGG AAAUAACGCCAAGAUCACC 23 12197

HSV2-UL30-3844 - UU UGGAAAUAACGCCAAGAUCACC 24 12198

HSV2-UL30-3845 - UCGUCAAGCAGUACGGCC 18 12199

HSV2-UL30-3846 - U UCGUCAAGCAGUACGGCC 19 12200

HSV2-UL30-1858 - C U U CG U C A AG CAGUACGGCC 20 4810

HSV2-UL30-3847 - CCUUCGUCAAGCAGUACGGCC 21 12201

HSV2-UL30-3848 - ACCUUCGUCAAGCAGUACGGCC 22 12202

HSV2-UL30-3849 - UGACCUUCGUCAAGCAGUACGGCC 24 12203

HSV2-UL30-3850 - UCGGAUCCUGCGACCUCC 18 12204

HSV2-UL30-3851 - CUCGGAUCCUGCGACCUCC 19 12205

HSV2-UL30-3852 - CGCUCGGAUCCUGCGACCUCC 21 12206 HSV2-UL30-3853 - UCGCUCGGAUCCUGCGACCUCC 22 12207

HSV2-UL30-3854 - UUCGCUCGGAUCCUGCGACCUCC 23 12208

HSV2-UL30-3855 - UUUCGCUCGGAUCCUGCGACCUCC 24 12209

HSV2-UL30-3856 - UGCCGGCCCCCGUCGUCC 18 12210

HSV2-UL30-3857 - CUGCCGGCCCCCGUCGUCC 19 12211

HSV2-UL30-516 - CCUGCCGGCCCCCGUCGUCC 20 3386

HSV2-UL30-3858 - UCCUCCCCCCGAUCAAGC 18 12212

HSV2-UL30-3859 - U UCCUCCCCCCGAUCAAGC 19 12213

HSV2-UL30-3860 - UGUUCCUCCCCCCGAUCAAGC 21 12214

HSV2-UL30-3861 - CUGU UCCUCCCCCCGAUCAAGC 22 12215

HSV2-UL30-3862 - CGCUGUUCCUCCCCCCGAUCAAGC 24 12216

HSV2-UL30-3863 - ACAUCCUCCUGUUUUCGC 18 12217

HSV2-UL30-3864 - CACAUCCUCCUGUU UUCGC 19 12218

HSV2-UL30-3865 - AGCACAUCCUCCUGU UUUCGC 21 12219

HSV2-UL30-3866 - CGAGCACAUCCUCCUGUU UUCGC 23 12220

HSV2-UL30-3867 - UCGAGCACAUCCUCCUGUU UUCGC 24 12221

HSV2-UL30-3868 - UACGGGCGCAUGAACGGC 18 12222

HSV2-UL30-3869 - CGGGUACGGGCGCAUGAACGGC 22 12223

HSV2-UL30-3870 - ACGGG UACGGGCGCAUGAACGGC 23 12224

HSV2-UL30-3871 - AAGAUGCUCAUCAAGGGC 18 12225

HSV2-UL30-3872 - CAAGAUGCUCAUCAAGGGC 19 12226

HSV2-UL30-3873 - CGUUGGGUACCAGGGGGC 18 12227

HSV2-UL30-3874 - ACGU UGGGUACCAGGGGGC 19 12228

HSV2-UL30-1947 - CACGU UGGGUACCAGGGGGC 20 4866

HSV2-UL30-3875 - CGGCACGU UGGGUACCAGGGGGC 23 12229

HSV2-UL30-3876 - CCGGCACG UUGGGUACCAGGGGGC 24 12230

HSV2-UL30-3877 - CGCCUGGCGGCGGCCCUGC 19 12231

HSV2-UL30-3878 - AGCGCCUGGCGGCGGCCCUGC 21 12232

HSV2-UL30-3879 - CGAGCGCCUGGCGGCGGCCCUGC 23 12233

HSV2-UL30-3880 - CCGAGCUGGCGGAGGAUC 18 12234

HSV2-UL30-3881 - UCCGAGCUGGCGGAGGAUC 19 12235

HSV2-UL30-3882 - UGUCCGAGCUGGCGGAGGAUC 21 12236

HSV2-UL30-3883 - UGGUGUCCGAGCUGGCGGAGGAUC 24 12237

HSV2-UL30-3884 - UGCGAAAGCAGAUCCGCUC 19 12238

HSV2-UL30-1967 - AUGCGAAAGCAGAUCCGCUC 20 4880

HSV2-UL30-3885 - CAUGCGAAAGCAGAUCCGCUC 21 12239

HSV2-UL30-3886 - CCAUGCGAAAGCAGAUCCGCUC 22 12240

HSV2-UL30-3887 - CGUCU UGAAGG AC A AG A AG 19 12241

HSV2-UL30-1881 - CCGUCU UGAAGGACAAGAAG 20 4829

HSV2-UL30-3888 - AGGCCGUCU UGAAGGACAAGAAG 23 12242

HSV2-UL30-3889 - UAGCCGAGCGCCCCGCAG 18 12243

HSV2-UL30-3890 - UUAGCCGAGCGCCCCGCAG 19 12244

HSV2-UL30-3891 - CGU UAGCCGAGCGCCCCGCAG 21 12245

HSV2-UL30-3892 - CG CG U U AG CCG AG CG CCCCG CAG 23 12246

HSV2-UL30-3893 - CCGCG UUAGCCGAGCGCCCCGCAG 24 12247

HSV2-UL30-3894 - UACAUGAACAAGGCGGAG 18 12248 HSV2-UL30-3895 - U U ACAUG AACAAGGCGG AG 19 12249

HSV2-UL30-1798 - U UUACAUGAACAAGGCGGAG 20 4774

HSV2-UL30-3896 - U UUUACAUGAACAAGGCGGAG 21 12250

HSV2-UL30-3897 - CUU UUACAUGAACAAGGCGGAG 22 12251

HSV2-UL30-3898 - ACU UUUACAUGAACAAGGCGGAG 23 12252

HSV2-UL30-3899 - UACUUUUACAUGAACAAGGCGGAG 24 12253

HSV2-UL30-3900 - AGACCGGGGGCCGGCACG 18 12254

HSV2-UL30-1943 - CGAGACCGGGGGCCGGCACG 20 4864

HSV2-UL30-3901 - CGCG AGACCGGGGGCCGGCACG 22 12255

HSV2-UL30-3902 - CGCGCG AGACCGGGGGCCGGCACG 24 12256

HSV2-UL30-3903 - UCGGAACCUCGAGCGACG 18 12257

HSV2-UL30-3904 - U UCGGAACCUCGAGCGACG 19 12258

HSV2-UL30-3905 - CGU UCGGAACCUCGAGCGACG 21 12259

HSV2-UL30-3906 - CGGCGU UCGGAACCUCGAGCGACG 24 12260

HSV2-UL30-3907 - CCGCGCGGCUCAGGGCCG 18 12261

HSV2-UL30-3908 - UGGCCGCGCGGCUCAGGGCCG 21 12262

HSV2-UL30-3909 - CGUGGCCGCGCGGCUCAGGGCCG 23 12263

HSV2-UL30-3910 - ACGUGGCCGCGCGGCUCAGGGCCG 24 12264

HSV2-UL30-3911 - CUCCGGGCCCGCGCAGCG 18 12265

HSV2-UL30-3912 - CCUCCGGGCCCGCGCAGCG 19 12266

HSV2-UL30-3913 - CGCCUCCGGGCCCGCGCAGCG 21 12267

HSV2-UL30-3914 - ACGCCUCCGGGCCCGCGCAGCG 22 12268

HSV2-UL30-3915 - UACGCCUCCGGGCCCGCGCAGCG 23 12269

HSV2-UL30-3916 - CUACGCCUCCGGGCCCGCGCAGCG 24 12270

HSV2-UL30-3917 - CUGGUGUCCGAGCUGGCG 18 12271

HSV2-UL30-2036 - UGCUGGUGUCCGAGCUGGCG 20 4927

HSV2-UL30-3918 - CUGCUGGUGUCCGAGCUGGCG 21 12272

HSV2-UL30-3919 - AGCUGCUGGUGUCCGAGCUGGCG 23 12273

HSV2-UL30-3920 - AAGCUGCUGGUGUCCGAGCUGGCG 24 12274

HSV2-UL30-3921 - CGCAGCGCGGGGUGAUCG 18 12275

HSV2-UL30-1886 - CGCGCAGCGCGGGGUGAUCG 20 4833

HSV2-UL30-3922 - CCGCGCAGCGCGGGGUGAUCG 21 12276

HSV2-UL30-3923 - CCCGCGCAGCGCGGGGUGAUCG 22 12277

HSV2-UL30-3924 - UACGGCGGAGGAAACUCG 18 12278

HSV2-UL30-3925 - CUACGGCGGAGGAAACUCG 19 12279

HSV2-UL30-3926 - CGCUACGGCGGAGGAAACUCG 21 12280

HSV2-UL30-3927 - CGGCGCUACGGCGGAGGAAACUCG 24 12281

HSV2-UL30-3928 - UCUACAAGGUCCCGCUCG 18 12282

HSV2-UL30-3929 - AUCUACAAGGUCCCGCUCG 19 12283

HSV2-UL30-3930 - AGAUCUACAAGGUCCCGCUCG 21 12284

HSV2-UL30-3931 - CGGAGAUCUACAAGGUCCCGCUCG 24 12285

HSV2-UL30-3932 - ACGUGCACGCGCGCUGGG 18 12286

HSV2-UL30-3933 - UACGUGCACGCGCGCUGGG 19 12287

HSV2-UL30-3934 - CGU ACGUGCACGCGCGCUGGG 21 12288

HSV2-UL30-3935 - CGCGU ACGUGCACGCGCGCUGGG 23 12289

HSV2-UL30-3936 - CGGGUCCUCGACCCCACCU 19 12290 HSV2-UL30-1948 - CCGGGUCCUCGACCCCACCU 20 4867

HSV2-UL30-3937 - CCCGGGUCCUCGACCCCACCU 21 12291

HSV2-UL30-3938 - UGGUGUGCAACUCGGUGU 18 12292

HSV2-UL30-3939 - AGGUGGUGUGCAACUCGGUGU 21 12293

HSV2-UL30-3940 - AAGGUGGUGUGCAACUCGGUGU 22 12294

HSV2-UL30-3941 - CAAGGUGGUGUGCAACUCGGUGU 23 12295

HSV2-UL30-3942 - UCAAGG UGGUGUGCAACUCGGUGU 24

12296

HSV2-UL30-3943 - CGGGACCGUCAUCACGCUU 19 12297

HSV2-UL30-1793 - CCGGGACCGUCAUCACGCUU 20 4770

HSV2-UL30-3944 - CGCCGGGACCGUCAUCACGCUU 22 12298

HSV2-UL30-3945 - CCGCCGGGACCGUCAUCACGCUU 23 12299

HSV2-UL30-3946 - CCCGCCGGGACCGUCAUCACGCU U 24 12300

Table 9G provides exemplary targeting domains for knocking out the UL30 gene selected according to the seven tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 9G

HSV2-UL30-3960 + UCUUGGCG UUAUU UCCAAA 19 12314

HSV2-UL30-2347 + AUCUUGGCGU UAU UUCCAAA 20 5128

HSV2-UL30-3961 + GAUCUUGGCGU UAUUUCCAAA 21 12315

HSV2-UL30-3962 + UGAUCU UGGCGU UAUU UCCAAA 22 12316

HSV2-UL30-3963 + GUGAUCU UGGCGUUAUUUCCAAA 23 12317

HSV2-UL30-3964 + GGUGAUCU UGGCGUUAUUUCCAAA 24 12318

HSV2-UL30-3965 + CCGCGGCACAAAACGAAA 18 12319

HSV2-UL30-3966 + GCCGCGGCACAAAACGAAA 19 12320

HSV2-UL30-2428 + GGCCGCGGCACAAAACGAAA 20 5181

HSV2-UL30-3967 + AG G CCG CGG CACAAA ACG A AA 21 12321

HSV2-UL30-3968 + GAGGCCGCGGCACAAAACGAAA 22 12322

HSV2-UL30-3969 + U G AG G CCG CG G CACAAA ACG AAA 23 12323

HSV2-UL30-3970 + GUGAGGCCGCGGCACAAAACGAAA 24 12324

HSV2-UL30-3971 + CGACGGCCUCGGGCCGCA 18 12325

HSV2-UL30-3972 + GCGACGGCCUCGGGCCGCA 19 12326

HSV2-UL30-1262 + CGCGACGGCCUCGGGCCGCA 20 4132

HSV2-UL30-3973 + GCGCGACGGCCUCGGGCCGCA 21 12327

HSV2-UL30-3974 + UGCGCGACGGCCUCGGGCCGCA 22 12328

HSV2-UL30-3975 + GUGCGCGACGGCCUCGGGCCGCA 23 12329

HSV2-UL30-3976 + GGUGCGCGACGGCCUCGGGCCGCA 24 12330

HSV2-UL30-3977 + GACAGGUCGUAGAGCAGA 18 12331

HSV2-UL30-3978 + GGACAGGUCGUAGAGCAGA 19 12332

HSV2-UL30-2551 + UGGACAGGUCGUAGAGCAGA 20 5269

HSV2-UL30-3979 + GUGGACAGGUCGUAGAGCAGA 21 12333

HSV2-UL30-3980 + GGUGGACAGGUCGUAGAGCAGA 22 12334

HSV2-UL30-3981 + UGG UGGACAGGUCGUAGAGCAGA 23 12335

HSV2-UL30-3982 + GUGGUGGACAGGUCGUAGAGCAGA 24

12336

HSV2-UL30-3983 + UGUCAAACUCCAGGACGA 18 12337

HSV2-UL30-3984 + CUGUCAAACUCCAGGACGA 19 12338

HSV2-UL30-1343 + GCUGUCAAACUCCAGGACGA 20 4213

HSV2-UL30-3985 + CGCUGUCAAACUCCAGGACGA 21 12339

HSV2-UL30-3986 + UCGCUGUCAAACUCCAGGACGA 22 12340

HSV2-UL30-3987 + UUCGCUGUCAAACUCCAGGACGA 23 12341

HSV2-UL30-3988 + AUUCGCUGUCAAACUCCAGGACGA 24 12342

HSV2-UL30-3989 + GGAGGUCGCAGGAUCCGA 18 12343

HSV2-UL30-3990 + GGGAGGUCGCAGGAUCCGA 19 12344

HSV2-UL30-2543 + GGGGAGG UCGCAGGAUCCGA 20 5262

HSV2-UL30-3991 + CGGGGAGGUCGCAGGAUCCGA 21 12345

HSV2-UL30-3992 + UCGGGGAGGUCGCAGGAUCCGA 22 12346

HSV2-UL30-3993 + CUCGGGGAGGUCGCAGGAUCCGA 23 12347

HSV2-UL30-3994 + ACUCGGGGAGGUCGCAGGAUCCGA 24 12348

HSV2-UL30-3995 + CUUCCGGGCGU UCCGCGA 18 12349

HSV2-UL30-3996 + UCUUCCGGGCGU UCCGCGA 19 12350

HSV2-UL30-2556 + GUCUUCCGGGCGUUCCGCGA 20 5273

HSV2-UL30-3997 + GGUCU UCCGGGCGU UCCGCGA 21 12351 HSV2-UL30-3998 + AGGUCU UCCGGGCGUUCCGCGA 22 12352

HSV2-UL30-3999 + GAGGUCU UCCGGGCGUUCCGCGA 23 12353

HSV2-UL30-4000 + CGAGGUCUUCCGGGCG UUCCGCGA 24 12354

HSV2-UL30-4001 + UGCGCCCGUACCCGUCGA 18 12355

HSV2-UL30-4002 + AUGCGCCCGUACCCGUCGA 19 12356

HSV2-UL30-2515 + CAUGCGCCCGUACCCGUCGA 20 5243

HSV2-UL30-4003 + UCAUGCGCCCGUACCCGUCGA 21 12357

HSV2-UL30-4004 + U UCA UGCGCCCGUACCCGUCGA 22 12358

HSV2-UL30-4005 + GUUCAUGCGCCCGUACCCGUCGA 23 12359

HSV2-UL30-4006 + CG U U CAUG CGCCCG U ACCCG U CG A 24 12360

HSV2-UL30-4007 + GCGGG ACAUCAGCU UCG A 18 12361

HSV2-UL30-4008 + UGCGGGACAUCAGCU UCGA 19 12362

HSV2-UL30-2879 + AUGCGGG ACAUCAGCU UCGA 20 11233

HSV2-UL30-4009 + AUGCGCAUGGAGUACGGA 18 12363

HSV2-UL30-4010 + GAUGCGCAUGGAGUACGGA 19 12364

HSV2-UL30-2433 + UG AUGCGCAUGGAGUACGGA 20 5184

HSV2-UL30-4011 + AUG AUGCGCAUGGAGUACGGA 21 12365

HSV2-UL30-4012 + GAUG AUGCGCAUGGAGUACGGA 22 12366

HSV2-UL30-4013 + AG AUG AUGCGCAUGGAGUACGGA 23 12367

HSV2-UL30-4014 + UAGAUGAUGCGCAUGGAGUACGGA 24 12368

HSV2-UL30-4015 + GAUCCGGUCCUUGAUGGA 18 12369

HSV2-UL30-4016 + GGAUCCGGUCCU UGAUGGA 19 12370

HSV2-UL30-1159 + GGGAUCCGGUCCUUGAUGGA 20 4029

HSV2-UL30-4017 + CGGGAUCCGGUCCUUGAUGGA 21 12371

HSV2-UL30-4018 + ACGGGAUCCGGUCCUUGAUGGA 22 12372

HSV2-UL30-4019 + UACGGGAUCCGGUCCU UGAUGGA 23 12373

HSV2-UL30-4020 + GUACGGGAUCCGGUCCUUGAUGGA 24 12374

HSV2-UL30-4021 + AAGUCGGCCAGCAGCUGA 18 12375

HSV2-UL30-4022 + AAAG UCGGCCAGCAGCUG A 19 12376

HSV2-UL30-2438 + GAAAGUCGGCCAGCAGCUGA 20 5186

HSV2-UL30-4023 + GGAAAGUCGGCCAGCAGCUGA 21 12377

HSV2-UL30-4024 + CGG AAAG UCGGCCAGCAGCUG A 22 12378

HSV2-UL30-4025 + CCGG AAAG UCGGCCAGCAGCUG A 23 12379

HSV2-UL30-4026 + U CCG G AAAG U CG G CC AG C AG C U G A 24 12380

HSV2-UL30-4027 + UUUCGCACUCGAGCUUGA 18 12381

HSV2-UL30-4028 + UU UUCGCACUCGAGCUUGA 19 12382

HSV2-UL30-2417 + UUU UUCGCACUCGAGCU UGA 20 5176

HSV2-UL30-4029 + UUU UUUCGCACUCGAGCUUGA 21 12383

HSV2-UL30-4030 + GUU UUUUCGCACUCGAGCU UGA 22 12384

HSV2-UL30-4031 + CGUUU UUUCGCACUCGAGCUUGA 23 12385

HSV2-UL30-4032 + ACGU UU UUUCGCACUCGAGCU UGA 24 12386

HSV2-UL30-4033 + AGUAAUAGUCCGUGU UGA 18 12387

HSV2-UL30-4034 + AAGUAAUAGUCCGUGU UGA 19 12388

HSV2-UL30-2352 + GAAGUAAUAGUCCGUGUUGA 20 5133

HSV2-UL30-4035 + AGAAGUAAUAGUCCGUGUUGA 21 12389

HSV2-UL30-4036 + GAGAAGUAAUAGUCCGUGUUGA 22 12390 HSV2-UL30-4037 + CGAGAAGUAAUAGUCCGUGUUGA 23 12391

HSV2-UL30-4038 + GCGAGAAGUAAUAGUCCGUGUUGA 24 12392

HSV2-UL30-4039 + CCGGCCUUGCAUUCGAUA 18 12393

HSV2-UL30-4040 + CCCGGCCUUGCAUUCGAUA 19 12394

HSV2-UL30-2558 + CCCCGGCCUUGCAU UCGAUA 20 5274

HSV2-UL30-4041 + CCCCCGGCCUUGCAU UCGAUA 21 12395

HSV2-UL30-4042 + CCCCCCGGCCUUGCAUUCGAUA 22 12396

HSV2-UL30-4043 + CCCCCCCGGCCUUGCAUUCGAUA 23 12397

HSV2-UL30-4044 + UCCCCCCCGGCCUUGCAUUCGAUA 24 12398

HSV2-UL30-4045 + GGCCGCAGGGAGAGCGUA 18 12399

HSV2-UL30-4046 + GGGCCGCAGGGAGAGCGUA 19 12400

HSV2-UL30-2468 + CGGGCCGCAGGGAGAGCGUA 20 5207

HSV2-UL30-4047 + UCGGGCCGCAGGGAGAGCGUA 21 12401

HSV2-UL30-4048 + CUCGGGCCGCAGGGAGAGCGUA 22 12402

HSV2-UL30-4049 + CCUCGGGCCGCAGGGAGAGCGUA 23 12403

HSV2-UL30-4050 + GCCUCGGGCCGCAGGGAGAGCGUA 24 12404

HSV2-UL30-4051 + CAGGAUCCGAGCGAAAAC 18 12405

HSV2-UL30-4052 + G C AG G A U CCG AG CG A A A AC 19 12406

HSV2-UL30-1359 + CGCAGGAUCCGAGCGAAAAC 20 4229

HSV2-UL30-4053 + UCGCAGGAUCCGAGCGAAAAC 21 12407

HSV2-UL30-4054 + GUCGCAGGAUCCGAGCGAAAAC 22 12408

HSV2-UL30-4055 + GG UCGCAGGAUCCGAGCGAAAAC 23 12409

HSV2-UL30-4056 + AGGUCGCAGGAUCCGAGCGAAAAC 24 12410

HSV2-UL30-4057 + UCCAGGUGCGGCAGAAAC 18 12411

HSV2-UL30-4058 + C U CC AG G U G CG G C AG A A AC 19 12412

HSV2-UL30-2499 + GCUCCAGGUGCGGCAGAAAC 20 5232

HSV2-UL30-4059 + AGCUCCAGGUGCGGCAGAAAC 21 12413

HSV2-UL30-4060 + AAGCUCCAGGUGCGGCAGAAAC 22 12414

HSV2-UL30-4061 + A A AG CU CC AG G UGCGGCAGAAAC 23 12415

HSV2-UL30-4062 + GAAAGCUCCAGGUGCGGCAG A A AC 24 12416

HSV2-UL30-4063 + ACGGGGUCGACGUGAAAC 18 12417

HSV2-UL30-4064 + CACGGGGUCGACGUGAAAC 19 12418

HSV2-UL30-2476 + CCACGGGGUCGACGUGAAAC 20 5213

HSV2-UL30-4065 + ACCACGGGGUCGACGUGAAAC 21 12419

HSV2-UL30-4066 + CACCACGGGG UCGACGUGAAAC 22 12420

HSV2-UL30-4067 + CCACCACGGGGUCGACGUGAAAC 23 12421

HSV2-UL30-4068 + ACCACCACGGGGUCGACGUGAAAC 24 12422

HSV2-UL30-4069 + UUGUACCCGGUCACGAAC 18 12423

HSV2-UL30-4070 + GUUGUACCCGGUCACGAAC 19 12424

HSV2-UL30-2522 + UGUUGUACCCGGUCACGAAC 20 5249

HSV2-UL30-4071 + AUGU UGUACCCGGUCACGAAC 21 12425

HSV2-UL30-4072 + GAUGU UGUACCCGGUCACGAAC 22 12426

HSV2-UL30-4073 + UGAUGUUGUACCCGGUCACGAAC 23 12427

HSV2-UL30-4074 + AUGAUGU UGUACCCGGUCACGAAC 24 12428

HSV2-UL30-4075 + CUGGCCGAUGUCCCACAC 18 12429

HSV2-UL30-4076 + UCUGGCCGAUGUCCCACAC 19 12430 HSV2-UL30-2512 + CUCUGGCCGAUGUCCCACAC 20 5241

HSV2-UL30-4077 + GCUCUGGCCGAUGUCCCACAC 21 12431

HSV2-UL30-4078 + GGCUCUGGCCGAUGUCCCACAC 22 12432

HSV2-UL30-4079 + UGGCUCUGGCCGAUGUCCCACAC 23 12433

HSV2-UL30-4080 + GUGGCUCUGGCCGAUGUCCCACAC 24 12434

HSV2-UL30-4081 + CAU UCGAUAUCGAAGCAC 18 12435

HSV2-UL30-4082 + GCAU UCG AU AUCG AAGCAC 19 12436

HSV2-UL30-2559 + UGCAUUCGAUAUCGAAGCAC 20 5275

HSV2-UL30-4083 + UUGCAUUCGAUAUCGAAGCAC 21 12437

HSV2-UL30-4084 + CUUGCAUUCGAUAUCGAAGCAC 22 12438

HSV2-UL30-4085 + CCUUGCAU UCGAUAUCGAAGCAC 23 12439

HSV2-UL30-4086 + GCCUUGCAUUCGAUAUCGAAGCAC 24 12440

HSV2-UL30-4087 + GUGAACG UUUUUUCGCAC 18 12441

HSV2-UL30-4088 + GGUGAACGUU UUUUCGCAC 19 12442

HSV2-UL30-2416 + UGGUGAACGU UUUU UCGCAC 20 5175

HSV2-UL30-4089 + UUGGUGAACGU UUUU UCGCAC 21 12443

HSV2-UL30-4090 + CU UGGUGAACGU UUUU UCGCAC 22 12444

HSV2-UL30-4091 + GCUUGGUGAACGUUU UUUCGCAC 23 12445

HSV2-UL30-4092 + AGCU UGGUGAACGU UU UUUCGCAC 24 12446

HSV2-UL30-4093 + GCAAGGCGCAGGAGGCAC 18 12447

HSV2-UL30-4094 + CG C A AG G CG C AG G AG G C AC 19 12448

HSV2-UL30-2493 + CCGCAAGGCGCAGGAGGCAC 20 5227

HSV2-UL30-4095 + CCCGCAAGGCGCAGGAGGCAC 21 12449

HSV2-UL30-4096 + GCCCG C A AG GCGCAGGAGGCAC 22 12450

HSV2-UL30-4097 + GGCCCGCAAGGCGCAGGAGGCAC 23 12451

HSV2-UL30-4098 + UGGCCCGCAAGGCGCAGGAGGCAC 24 12452

HSV2-UL30-4099 + ACAGGUCGUAGAGCAGAC 18 12453

HSV2-UL30-4100 + GACAGGUCGUAGAGCAGAC 19 12454

HSV2-UL30-1367 + GGACAGGUCG UAGAGCAGAC 20 4237

HSV2-UL30-4101 + UGG ACAGGUCGUAGAGCAGAC 21 12455

HSV2-UL30-4102 + GUGG ACAGGUCGUAGAGCAGAC 22 12456

HSV2-UL30-4103 + GG UGG ACAGGUCGUAGAGCAGAC 23 12457

HSV2-UL30-4104 + UGGUGGACAGGUCGUAGAGCAGAC 24 12458

HSV2-UL30-4105 + CUGCCGCGUGCCGUAGAC 18 12459

HSV2-UL30-4106 + ACUGCCGCGUGCCGUAGAC 19 12460

HSV2-UL30-2587 + UACUGCCGCGUGCCGUAGAC 20 5296

HSV2-UL30-4107 + GUACUGCCGCGUGCCGUAGAC 21 12461

HSV2-UL30-4108 + AGUACUGCCGCGUGCCGUAGAC 22 12462

HSV2-UL30-4109 + AAG UACUGCCGCGUGCCGUAGAC 23 12463

HSV2-UL30-4110 + AAAGUACUGCCGCGUGCCGUAGAC 24 12464

HSV2-UL30-4111 + GUCAAACUCCAGGACGAC 18 12465

HSV2-UL30-4112 + UGUCAAACUCCAGGACGAC 19 12466

HSV2-UL30-1344 + CUGUCAAACUCCAGGACGAC 20 4214

HSV2-UL30-4113 + GCUGUCAAACUCCAGGACGAC 21 12467

HSV2-UL30-4114 + CGCUGUCAAACUCCAGGACGAC 22 12468

HSV2-UL30-4115 + UCGCUGUCAAACUCCAGGACGAC 23 12469 HSV2-UL30-4116 + UUCGCUGUCAAACUCCAGGACGAC 24 12470

HSV2-UL30-4117 + UUCCGGGCGUUCCGCGAC 18 12471

HSV2-UL30-4118 + CU UCCGGGCGUUCCGCGAC 19 12472

HSV2-UL30-1372 + UCUUCCGGGCGU UCCGCGAC 20 4242

HSV2-UL30-4119 + GUCUUCCGGGCGUUCCGCGAC 21 12473

HSV2-UL30-4120 + GGUCUUCCGGGCGUUCCGCGAC 22 12474

HSV2-UL30-4121 + AGGUCU UCCGGGCGU UCCGCGAC 23 12475

HSV2-UL30-4122 + GAGGUCUUCCGGGCGU UCCGCGAC 24 12476

HSV2-UL30-4123 + GUAAAACAGCAGGUCGAC 18 12477

HSV2-UL30-4124 + CGUAAAACAGCAGGUCGAC 19 12478

HSV2-UL30-2410 + UCGUAAAACAGCAGGUCGAC 20 5170

HSV2-UL30-4125 + GUCG U AAAACAGCAGG U CG AC 21 12479

HSV2-UL30-4126 + CGUCGUAAAACAGCAGGUCGAC 22 12480

HSV2-UL30-4127 + UCGUCGUAAAACAGCAGGUCGAC 23 12481

HSV2-UL30-4128 + AUCG UCGUAAAACAGCAGGUCGAC 24 12482

HSV2-UL30-4129 + CACCACCACGGGGUCGAC 18 12483

HSV2-UL30-4130 + ACACCACCACGGGGUCGAC 19 12484

HSV2-UL30-2475 + AACACCACCACGGGGUCGAC 20 5212

HSV2-UL30-4131 + A A AC ACC ACC ACG GGGUCGAC 21 12485

HSV2-UL30-4132 + CAAACACCACCACGGGGUCGAC 22 12486

HSV2-UL30-4133 + U C A A AC ACC ACC ACG GGGUCGAC 23 12487

HSV2-UL30-4134 + G U C A A AC ACC ACC ACG G G G U CG AC 24 12488

HSV2-UL30-4135 + CGGGACAUCAGCUUCGAC 18 12489

HSV2-UL30-4136 + GCGGG ACAUCAGCU UCG AC 19 12490

HSV2-UL30-2880 + UGCGGGACAUCAGCUUCGAC 20 11234

HSV2-UL30-4137 + GGAGGUGGGGUCGAGGAC 18 12491

HSV2-UL30-4138 + CGGAGGUGGGGUCGAGGAC 19 12492

HSV2-UL30-2482 + CCGGAGGUGGGGUCGAGGAC 20 5217

HSV2-UL30-4139 + CCCGGAGGUGGGGUCGAGGAC 21 12493

HSV2-UL30-4140 + ACCCGGAGGUGGGGUCGAGGAC 22 12494

HSV2-UL30-4141 + AACCCGGAGGUGGGGUCGAGGAC 23 12495

HSV2-UL30-4142 + AAACCCGGAGGUGGGGUCGAGGAC 24 12496

HSV2-UL30-4143 + UGCGCAUGGAGUACGGAC 18 12497

HSV2-UL30-4144 + AUGCGCAUGGAGUACGGAC 19 12498

HSV2-UL30-1218 + GAUGCGCAUGGAGUACGGAC 20 4088

HSV2-UL30-4145 + UGAUGCGCAUGGAGUACGGAC 21 12499

HSV2-UL30-4146 + AUG AUGCGCAUGGAGUACGGAC 22 12500

HSV2-UL30-4147 + G AUG AUGCGCAUGGAGUACGGAC 23 12501

HSV2-UL30-4148 + AGAUGAUGCGCAUGGAGUACGGAC 24 12502

HSV2-UL30-4149 + AGAUCGCUGAGGUGGGAC 18 12503

HSV2-UL30-4150 + GAGAUCGCUGAGGUGGGAC 19 12504

HSV2-UL30-2537 + CG AGAUCGCUGAGGUGGGAC 20 5259

HSV2-UL30-4151 + GCGAGAUCGCUGAGGUGGGAC 21 12505

HSV2-UL30-4152 + GGCG AGAUCGCUGAGGUGGGAC 22 12506

HSV2-UL30-4153 + AGGCG AGAUCGCUGAGGUGGGAC 23 12507

HSV2-UL30-4154 + GAGGCGAGAUCGCUGAGGUGGGAC 24 12508 HSV2-UL30-4155 + GCGCCGAUGGGCGUCUAC 18 12509

HSV2-UL30-4156 + UGCGCCGAUGGGCGUCUAC 19 12510

HSV2-UL30-2402 + AUGCGCCGAUGGGCGUCUAC 20 5164

HSV2-UL30-4157 + GAUGCGCCGAUGGGCGUCUAC 21 12511

HSV2-UL30-4158 + UGAUGCGCCGAUGGGCGUCUAC 22 12512

HSV2-UL30-4159 + GUGAUGCGCCGAUGGGCGUCUAC 23 12513

HSV2-UL30-4160 + GGUGAUGCGCCGAUGGGCGUCUAC 24 12514

HSV2-UL30-4161 + CAGCAGGCUCUCGCGUAC 18 12515

HSV2-UL30-4162 + UCAGCAGGCUCUCGCGUAC 19 12516

HSV2-UL30-2460 + CUCAGCAGGCUCUCGCGUAC 20 5201

HSV2-UL30-4163 + GCUCAGCAGGCUCUCGCGUAC 21 12517

HSV2-UL30-4164 + UGCUCAGCAGGCUCUCGCGUAC 22 12518

HSV2-UL30-4165 + AUGCUCAGCAGGCUCUCGCGUAC 23 12519

HSV2-UL30-4166 + GAUGCUCAGCAGGCUCUCGCGUAC 24 12520

HSV2-UL30-4167 + CCCCGGGCGAUGGCGUAC 18 12521

HSV2-UL30-4168 + GCCCCGGGCGAUGGCGUAC 19 12522

HSV2-UL30-2355 + CGCCCCGGGCGAUGGCG UAC 20 5135

HSV2-UL30-4169 + ACGCCCCGGGCGAUGGCGUAC 21 12523

HSV2-UL30-4170 + AACGCCCCGGGCGAUGGCGUAC 22 12524

HSV2-UL30-4171 + GAACGCCCCGGGCGAUGGCGUAC 23 12525

HSV2-UL30-4172 + GGAACGCCCCGGGCGAUGGCGUAC 24 12526

HSV2-UL30-4173 + CGGGGUCGACGUGAAACC 18 12527

HSV2-UL30-4174 + ACGGGGUCGACGUGAAACC 19 12528

HSV2-UL30-1275 + CACGGGGUCGACGUGAAACC 20 4145

HSV2-UL30-4175 + CCACGGGGUCGACGUGAAACC 21 12529

HSV2-UL30-4176 + ACCACGGGGUCGACGUGAAACC 22 12530

HSV2-UL30-4177 + CACCACGGGGUCGACGUG A A ACC 23 12531

HSV2-UL30-4178 + CCACCACGGGGUCGACGUGAAACC 24 12532

HSV2-UL30-4179 + G CAAAG U CAA ACACCACC 18 12533

HSV2-UL30-4180 + GGCAAAGUCAAACACCACC 19 12534

HSV2-UL30-2473 + U GG CAA AG U CAAAC ACCACC 20 5211

HSV2-UL30-4181 + CUGGCAAAGUCAAACACCACC 21 12535

HSV2-UL30-4182 + G CU GGCAAAG U CA AACACC ACC 22 12536

HSV2-UL30-4183 + G G C U G G C A A AG U C A A AC ACC ACC 23 12537

HSV2-UL30-4184 + AGGCUGGCAAAGU CAA AC ACC ACC 24 12538

HSV2-UL30-4185 + GCCUUCGGGGGUCAGACC 18 12539

HSV2-UL30-4186 + GGCCU UCGGGGGUCAGACC 19 12540

HSV2-UL30-2591 + UGGCCUUCGGGGGUCAGACC 20 5298

HSV2-UL30-4187 + AUGGCCUUCGGGGGUCAGACC 21 12541

HSV2-UL30-4188 + GAUGGCCUUCGGGGGUCAGACC 22 12542

HSV2-UL30-4189 + CG AUGGCCU UCGGGGGUCAGACC 23 12543

HSV2-UL30-4190 + GCGAUGGCCUUCGGGGGUCAGACC 24 12544

HSV2-UL30-4191 + GCGCAUGGAGUACGGACC 18 12545

HSV2-UL30-4192 + UGCGCAUGGAGUACGGACC 19 12546

HSV2-UL30-1219 + AUGCGCAUGGAGUACGGACC 20 4089

HSV2-UL30-4193 + GAUGCGCAUGGAGUACGGACC 21 12547 HSV2-UL30-4194 + UGAUGCGCAUGGAGUACGGACC 22 12548

HSV2-UL30-4195 + AUGAUGCGCAUGGAGUACGGACC 23 12549

HSV2-UL30-4196 + GAUGAUGCGCAUGGAGUACGGACC 24 12550

HSV2-UL30-4197 + CGGGGGCCGGCAGGCCCC 18 12551

HSV2-UL30-4198 + ACGGGGGCCGGCAGGCCCC 19 12552

HSV2-UL30-1349 + GACGGGGGCCGGCAGGCCCC 20 4219

HSV2-UL30-4199 + CG ACGGGGGCCGGCAGGCCCC 21 12553

HSV2-UL30-4200 + ACG ACGGGGGCCGGCAGGCCCC 22 12554

HSV2-UL30-4201 + GACG ACGGGGGCCGGCAGGCCCC 23 12555

HSV2-UL30-4202 + GG ACG ACGGGGGCCGGCAGGCCCC 24 12556

HSV2-UL30-4203 + GGGGGCGGGCUCGUCCCC 18 12557

HSV2-UL30-4204 + GGGGGGCGGGCUCGUCCCC 19 12558

HSV2-UL30-1142 + GGGGGGGCGGGCUCGUCCCC 20 4012

HSV2-UL30-4205 + UGGGGGGGCGGGCUCGUCCCC 21 12559

HSV2-UL30-4206 + CUGGGGGGGCGGGCUCGUCCCC 22 12560

HSV2-UL30-4207 + GCUGGGGGGGCGGGCUCGUCCCC 23 12561

HSV2-UL30-4208 + CGCUGGGGGGGCGGGCUCGUCCCC 24 12562

HSV2-UL30-4209 + CGGCCGCUCCCCUUCCCC 18 12563

HSV2-UL30-4210 + CCGGCCGCUCCCCUUCCCC 19 12564

HSV2-UL30-2483 + CCCGGCCGCUCCCCUUCCCC 20 5218

HSV2-UL30-4211 + CCCCGGCCGCUCCCCU UCCCC 21 12565

HSV2-UL30-4212 + CCCCCGGCCGCUCCCCU UCCCC 22 12566

HSV2-UL30-4213 + UCCCCCGGCCGCUCCCCUUCCCC 23 12567

HSV2-UL30-4214 + GUCCCCCGGCCGCUCCCCUUCCCC 24 12568

HSV2-UL30-4215 + ACGGGGGCCGGCAGGCCC 18 12569

HSV2-UL30-4216 + GACGGGGGCCGGCAGGCCC 19 12570

HSV2-UL30-2531 + CGACGGGGGCCGGCAGGCCC 20 5255

HSV2-UL30-4217 + ACG ACGGGGGCCGGCAGGCCC 21 12571

HSV2-UL30-4218 + GACG ACGGGGGCCGGCAGGCCC 22 12572

HSV2-UL30-4219 + GGACG ACGGGGGCCGGCAGGCCC 23 12573

HSV2-UL30-4220 + AGGACGACGGGGGCCGGCAGGCCC 24 12574

HSV2-UL30-4221 + GCAGGUCGACCAGGGCCC 18 12575

HSV2-UL30-4222 + AGCAGGUCGACCAGGGCCC 19 12576

HSV2-UL30-1198 + C AG CAGGUCGACCAGGGCCC 20 4068

HSV2-UL30-4223 + ACAG CAG G U CG ACCAG G G CCC 21 12577

HSV2-UL30-4224 + AACAGCAGGUCGACCAGGGCCC 22 12578

HSV2-UL30-4225 + AAACAGCAGGUCGACCAGGGCCC 23 12579

HSV2-UL30-4226 + AAAACAGCAGGUCGACCAGGGCCC 24 12580

HSV2-UL30-4227 + GGCUUGGACGCGCCUCCC 18 12581

HSV2-UL30-4228 + GGGCU UGGACGCGCCUCCC 19 12582

HSV2-UL30-1116 + GGGGCUUGGACGCGCCUCCC 20 3986

HSV2-UL30-4229 + CGGGGCUUGGACGCGCCUCCC 21 12583

HSV2-UL30-4230 + GCGGGGCUUGGACGCGCCUCCC 22 12584

HSV2-UL30-4231 + UGCGGGGCUUGGACGCGCCUCCC 23 12585

HSV2-UL30-4232 + UUGCGGGGCU UGGACGCGCCUCCC 24 12586

HSV2-UL30-4233 + CAUGCGACGGCGUCUCCC 18 12587 HSV2-UL30-4234 + GCAUGCGACGGCGUCUCCC 19 12588

HSV2-UL30-1123 + GGCAUGCGACGGCGUCUCCC 20 3993

HSV2-UL30-4235 + CGGCAUGCGACGGCGUCUCCC 21 12589

HSV2-UL30-4236 + UCGGCAUGCGACGGCGUCUCCC 22 12590

HSV2-UL30-4237 + GUCGGCAUGCGACGGCGUCUCCC 23 12591

HSV2-UL30-4238 + GGUCGGCAUGCGACGGCGUCUCCC 24 12592

HSV2-UL30-4239 + CCG AACGCCU GCAG U CCC 18 12593

HSV2-UL30-4240 + CCCGAACGCCUGCAGUCCC 19 12594

HSV2-UL30-2404 + CCCCG AACGCCU GCAG U CCC 20 5166

HSV2-UL30-4241 + GCCCCGAACGCCUGCAGUCCC 21 12595

HSV2-UL30-4242 + GGCCCCGAACGCCUGCAGUCCC 22 12596

HSV2-UL30-4243 + CGGCCCCGAACGCCUGCAGUCCC 23 12597

HSV2-UL30-4244 + ACGGCCCCGAACGCCUGCAGUCCC 24 12598

HSV2-UL30-4245 + GGGGGGCGGGCUCGUCCC 18 12599

HSV2-UL30-4246 + GGGGGGGCGGGCUCGUCCC 19 12600

HSV2-UL30-2380 + UGGGGGGGCGGGCUCG UCCC 20 5146

HSV2-UL30-4247 + CUGGGGGGGCGGGCUCGUCCC 21 12601

HSV2-UL30-4248 + GCUGGGGGGGCGGGCUCGUCCC 22 12602

HSV2-UL30-4249 + CGCUGGGGGGGCGGGCUCGUCCC 23 12603

HSV2-UL30-4250 + CCGCUGGGGGGGCGGGCUCGUCCC 24 12604

HSV2-UL30-4251 + CGUGUUGAGCGGAACGCC 18 12605

HSV2-UL30-4252 + CCGUGUUGAGCGGAACGCC 19 12606

HSV2-UL30-2354 + UCCGUGUUGAGCGGAACGCC 20 5134

HSV2-UL30-4253 + GUCCGUGU UGAGCGGAACGCC 21 12607

HSV2-UL30-4254 + AGUCCGUGUUGAGCGGAACGCC 22 12608

HSV2-UL30-4255 + UAGUCCGUGUUGAGCGGAACGCC 23 12609

HSV2-UL30-4256 + AUAGUCCGUGU UGAGCGGAACGCC 24 12610

HSV2-UL30-4257 + CG CU CCACC ACCU CCG CC 18 12611

HSV2-UL30-4258 + GCGCUCCACCACCUCCGCC 19 12612

HSV2-UL30-2578 + CGCGCUCCACCACCUCCGCC 20 5290

HSV2-UL30-4259 + GCGCGCUCCACCACCUCCGCC 21 12613

HSV2-UL30-4260 + GGCGCGCUCCACCACCUCCGCC 22 12614

HSV2-UL30-4261 + CGGCGCGCUCCACCACCUCCGCC 23 12615

HSV2-UL30-4262 + UCGGCGCGCUCCACCACCUCCGCC 24 12616

HSV2-UL30-4263 + CGCCUCCUUGUCGAGGCC 18 12617

HSV2-UL30-4264 + GCGCCUCCUUG UCGAGGCC 19 12618

HSV2-UL30-2487 + GGCGCCUCCUUGUCGAGGCC 20 5222

HSV2-UL30-4265 + GGGCGCCUCCU UGUCGAGGCC 21 12619

HSV2-UL30-4266 + UGGGCGCCUCCU UGUCGAGGCC 22 12620

HSV2-UL30-4267 + UUGGGCGCCUCCUUGUCGAGGCC 23 12621

HSV2-UL30-4268 + CUUGGGCGCCUCCUUGUCGAGGCC 24 12622

HSV2-UL30-4269 + UCCAGGUGCGCGACGGCC 18 12623

HSV2-UL30-4270 + CUCCAGGUGCGCGACGGCC 19 12624

HSV2-UL30-2463 + CCUCCAGGUGCGCGACGGCC 20 5204

HSV2-UL30-4271 + GCCUCCAGGUGCGCGACGGCC 21 12625

HSV2-UL30-4272 + CGCCUCCAGGUGCGCGACGGCC 22 12626 HSV2-UL30-4273 + CCGCCUCCAGGUGCGCGACGGCC 23 12627

HSV2-UL30-4274 + UCCGCCUCCAGGUGCGCGACGGCC 24 12628

HSV2-UL30-4275 + GCGUCUACGAGGACGGCC 18 12629

HSV2-UL30-4276 + GGCGUCUACGAGGACGGCC 19 12630

HSV2-UL30-2403 + GGGCGUCUACGAGGACGGCC 20 5165

HSV2-UL30-4277 + UGGGCGUCUACGAGGACGGCC 21 12631

HSV2-UL30-4278 + AUGGGCGUCUACGAGGACGGCC 22 12632

HSV2-UL30-4279 + GAUGGGCGUCUACGAGGACGGCC 23 12633

HSV2-UL30-4280 + CGAUGGGCGUCUACGAGGACGGCC 24 12634

HSV2-UL30-4281 + GGCCCGAACCCCGCGGCC 18 12635

HSV2-UL30-4282 + CGGCCCGAACCCCGCGGCC 19 12636

HSV2-UL30-2339 + CCGGCCCGAACCCCGCGGCC 20 5125

HSV2-UL30-4283 + GCCGGCCCGAACCCCGCGGCC 21 12637

HSV2-UL30-4284 + CGCCGGCCCGAACCCCGCGGCC 22 12638

HSV2-UL30-4285 + CCGCCGGCCCGAACCCCGCGGCC 23 12639

HSV2-UL30-4286 + CCCGCCGGCCCGAACCCCGCGGCC 24 12640

HSV2-UL30-4287 + GCCACCAGGCCCGCGGCC 18 12641

HSV2-UL30-4288 + GGCCACCAGGCCCGCGGCC 19 12642

HSV2-UL30-2426 + UGGCCACCAGGCCCGCGGCC 20 5179

HSV2-UL30-4289 + AUGGCCACCAGGCCCGCGGCC 21 12643

HSV2-UL30-4290 + CAUGGCCACCAGGCCCGCGGCC 22 12644

HSV2-UL30-4291 + CCAUGGCCACCAGGCCCGCGGCC 23 12645

HSV2-UL30-4292 + CCCAUGGCCACCAGGCCCGCGGCC 24 12646

HSV2-UL30-4293 + UUAU UUCCAAACAGGGCC 18 12647

HSV2-UL30-4294 + G U U AU U U CC AAAC AG G G CC 19 12648

HSV2-UL30-2348 + CG UUAUUUCCAAACAGGGCC 20 5129

HSV2-UL30-4295 + GCGUUAUUUCCAAACAGGGCC 21 12649

HSV2-UL30-4296 + GGCGUUAUUU CCA A AC AG G G CC 22 12650

HSV2-UL30-4297 + UGGCGUUAUUUCCAAACAGGGCC 23 12651

HSV2-UL30-4298 + UUGGCGUUAUU UCCAAACAGGGCC 24 12652

HSV2-UL30-4299 + AGCAGGUCGACCAGGGCC 18 12653

HSV2-UL30-4300 + CAGCAGGUCGACCAGGGCC 19 12654

HSV2-UL30-2411 + ACAGCAGGUCGACCAGGGCC 20 5171

HSV2-UL30-4301 + AACAGCAGGUCGACCAGGGCC 21 12655

HSV2-UL30-4302 + AAACAGCAGGUCGACCAGGGCC 22 12656

HSV2-UL30-4303 + A A A AC AG CAGGUCGACCAGGGCC 23 12657

HSV2-UL30-4304 + UAAAACAGCAGGUCGACCAGGGCC 24 12658

HSV2-UL30-4305 + CCCCGCGCUGCGCGGGCC 18 12659

HSV2-UL30-4306 + ACCCCGCGCUGCGCGGGCC 19 12660

HSV2-UL30-1314 + CACCCCGCGCUGCGCGGGCC 20 4184

HSV2-UL30-4307 + UCACCCCGCGCUGCGCGGGCC 21 12661

HSV2-UL30-4308 + AUCACCCCGCGCUGCGCGGGCC 22 12662

HSV2-UL30-4309 + GAUCACCCCGCGCUGCGCGGGCC 23 12663

HSV2-UL30-4310 + CGAUCACCCCGCGCUGCGCGGGCC 24 12664

HSV2-UL30-4311 + ACCUGCGCGCGGCGGGCC 18 12665

HSV2-UL30-4312 + GACCUGCGCGCGGCGGGCC 19 12666 HSV2-UL30-2393 + GGACCUGCGCGCGGCGGGCC 20 5155

HSV2-UL30-4313 + GGGACCUGCGCGCGGCGGGCC 21 12667

HSV2-UL30-4314 + CGGGACCUGCGCGCGGCGGGCC 22 12668

HSV2-UL30-4315 + ACGGGACCUGCGCGCGGCGGGCC 23 12669

HSV2-UL30-4316 + GACGGGACCUGCGCGCGGCGGGCC 24 12670

HSV2-UL30-4317 + UCCCGGGGGCGCU UGGCC 18 12671

HSV2-UL30-4318 + CUCCCGGGGGCGCUUGGCC 19 12672

HSV2-UL30-1128 + UCUCCCGGGGGCGCU UGGCC 20 3998

HSV2-UL30-4319 + GUCUCCCGGGGGCGCU UGGCC 21 12673

HSV2-UL30-4320 + CGUCUCCCGGGGGCGCU UGGCC 22 12674

HSV2-UL30-4321 + GCGUCUCCCGGGGGCGCUUGGCC 23 12675

HSV2-UL30-4322 + GGCGUCUCCCGGGGGCGCUUGGCC 24 12676

HSV2-UL30-4323 + GUGGUCCGCGGAGAUGCC 18 12677

HSV2-UL30-4324 + AGUGGUCCGCGGAGAUGCC 19 12678

HSV2-UL30-2581 + AAGUGGUCCGCGGAGAUGCC 20 5292

HSV2-UL30-4325 + GAAGUGGUCCGCGGAGAUGCC 21 12679

HSV2-UL30-4326 + CGAAGUGGUCCGCGGAGAUGCC 22 12680

HSV2-UL30-4327 + UCGAAGUGGUCCGCGGAGAUGCC 23 12681

HSV2-UL30-4328 + CUCGAAGUGGUCCGCGGAGAUGCC 24 12682

HSV2-UL30-4329 + GGGUGCUCUGGGGGAUCC 18 12683

HSV2-UL30-4330 + GGGGUGCUCUGGGGGAUCC 19 12684

HSV2-UL30-2457 + GGGGGUGCUCUGGGGGAUCC 20 5198

HSV2-UL30-4331 + CGGGGGUGCUCUGGGGGAUCC 21 12685

HSV2-UL30-4332 + UCGGGGGUGCUCUGGGGGAUCC 22 12686

HSV2-UL30-4333 + CUCGGGGGUGCUCUGGGGGAUCC 23 12687

HSV2-UL30-4334 + CCUCGGGGGUGCUCUGGGGGAUCC 24 12688

HSV2-UL30-4335 + GGGCUUGGACGCGCCUCC 18 12689

HSV2-UL30-4336 + GGGGCUUGGACGCGCCUCC 19 12690

HSV2-UL30-1115 + CGGGGCUUGGACGCGCCUCC 20 3985

HSV2-UL30-4337 + GCGGGGCUUGGACGCGCCUCC 21 12691

HSV2-UL30-4338 + UGCGGGGCUUGGACGCGCCUCC 22 12692

HSV2-UL30-4339 + UUGCGGGGCUUGGACGCGCCUCC 23 12693

HSV2-UL30-4340 + CU UGCGGGGCUUGGACGCGCCUCC 24 12694

HSV2-UL30-4341 + UCGAGGAGGACGGCCUCC 18 12695

HSV2-UL30-4342 + GUCGAGGAGGACGGCCUCC 19 12696

HSV2-UL30-2450 + UG UCGAGGAGGACGGCCUCC 20 5196

HSV2-UL30-4343 + U UGUCGAGGAGGACGGCCUCC 21 12697

HSV2-UL30-4344 + CUUGUCGAGGAGGACGGCCUCC 22 12698

HSV2-UL30-4345 + GCU UG UCGAGGAGGACGGCCUCC 23 12699

HSV2-UL30-4346 + UGCUUGUCGAGGAGGACGGCCUCC 24 12700

HSV2-UL30-4347 + GCAUGCGACGGCGUCUCC 18 12701

HSV2-UL30-4348 + GGCAUGCGACGGCGUCUCC 19 12702

HSV2-UL30-1122 + CGGCAUGCGACGGCGUCUCC 20 3992

HSV2-UL30-4349 + UCGGCAUGCGACGGCGUCUCC 21 12703

HSV2-UL30-4350 + GUCGGCAUGCGACGGCG UCUCC 22 12704

HSV2-UL30-4351 + GGUCGGCAUGCGACGGCGUCUCC 23 12705 HSV2-UL30-4352 + GGGUCGGCAUGCGACGGCGUCUCC 24 12706

HSV2-UL30-4353 + CGCGCGGCCACGUCGUCC 18 12707

HSV2-UL30-4354 + CCGCGCGGCCACGUCGUCC 19 12708

HSV2-UL30-1092 + GCCGCGCGGCCACGUCGUCC 20 3962

HSV2-UL30-4355 + AGCCGCGCGGCCACGUCGUCC 21 12709

HSV2-UL30-4356 + GAGCCGCGCGGCCACGUCGUCC 22 12710

HSV2-UL30-4357 + UGAGCCGCGCGGCCACGUCGUCC 23 12711

HSV2-UL30-4358 + CUGAGCCGCGCGGCCACGUCGUCC 24 12712

HSV2-UL30-4359 + UCCGCCUCGAAGUGGUCC 18 12713

HSV2-UL30-4360 + CUCCGCCUCGAAGUGGUCC 19 12714

HSV2-UL30-2579 + CCUCCGCCUCGAAGUGG UCC 20 5291

HSV2-UL30-4361 + ACCUCCGCCUCGAAGUGGUCC 21 12715

HSV2-UL30-4362 + CACCUCCGCCUCGAAGUGGUCC 22 12716

HSV2-UL30-4363 + CCACCUCCGCCUCGAAGUGGUCC 23 12717

HSV2-UL30-4364 + ACCACCUCCGCCUCGAAGUGGUCC 24 12718

HSV2-UL30-4365 + CCGGGAUCCUCCGCCAGC 18 12719

HSV2-UL30-4366 + CCCGGGAUCCUCCGCCAGC 19 12720

HSV2-UL30-2357 + ACCCGGGAUCCUCCGCCAGC 20 5136

HSV2-UL30-4367 + UACCCGGGAUCCUCCGCCAGC 21 12721

HSV2-UL30-4368 + GUACCCGGGAUCCUCCGCCAGC 22 12722

HSV2-UL30-4369 + CGUACCCGGGAUCCUCCGCCAGC 23 12723

HSV2-UL30-4370 + GCGUACCCGGGAUCCUCCGCCAGC 24 12724

HSV2-UL30-4371 + CGGCGUUCAGCUUGUAGC 18 12725

HSV2-UL30-4372 + ACGGCGUUCAGCUUGUAGC 19 12726

HSV2-UL30-1324 + GACGGCGUUCAGCUUGUAGC 20 4194

HSV2-UL30-4373 + CGACGGCGUUCAGCU UGUAGC 21 12727

HSV2-UL30-4374 + GCGACGGCGU UCAGCUUGUAGC 22 12728

HSV2-UL30-4375 + GGCGACGGCGU UCAGCUUGUAGC 23 12729

HSV2-UL30-4376 + CGGCGACGGCGUUCAGCU UGUAGC 24 12730

HSV2-UL30-4377 + CGGGGAGGGCAGGGCCGC 18 12731

HSV2-UL30-4378 + CCGGGGAGGGCAGGGCCGC 19 12732

HSV2-UL30-1134 + GCCGGGGAGGGCAGGGCCGC 20 4004

HSV2-UL30-4379 + GGCCGGGGAGGGCAGGGCCGC 21 12733

HSV2-UL30-4380 + UGGCCGGGGAGGGCAGGGCCGC 22 12734

HSV2-UL30-4381 + UUGGCCGGGGAGGGCAGGGCCGC 23 12735

HSV2-UL30-4382 + CUUGGCCGGGGAGGGCAGGGCCGC 24 12736

HSV2-UL30-4383 + GCGACGGCCUCGGGCCGC 18 12737

HSV2-UL30-4384 + CGCGACGGCCUCGGGCCGC 19 12738

HSV2-UL30-1261 + GCGCGACGGCCUCGGGCCGC 20 4131

HSV2-UL30-4385 + UGCGCGACGGCCUCGGGCCGC 21 12739

HSV2-UL30-4386 + GUGCGCGACGGCCUCGGGCCGC 22 12740

HSV2-UL30-4387 + GG UGCGCGACGGCCUCGGGCCGC 23 12741

HSV2-UL30-4388 + AGGUGCGCGACGGCCUCGGGCCGC 24 12742

HSV2-UL30-4389 + GGGGGAGGAACAGCGCGC 18 12743

HSV2-UL30-4390 + GGGGGGAGGAACAGCGCGC 19 12744

HSV2-UL30-2425 + CGGGGGGAGGAACAGCGCGC 20 5178 HSV2-UL30-4391 + UCGGGGGGAGGAACAGCGCGC 21 12745

HSV2-UL30-4392 + AUCGGGGGGAGGAACAGCGCGC 22 12746

HSV2-UL30-4393 + GAUCGGGGGGAGGAACAGCGCGC 23 12747

HSV2-UL30-4394 + UGAUCGGGGGGAGGAACAGCGCGC 24 12748

HSV2-UL30-4395 + UUCUGGCCCGCAAGGCGC 18 12749

HSV2-UL30-4396 + CUUCUGGCCCGCAAGGCGC 19 12750

HSV2-UL30-1300 + CCUUCUGGCCCGCAAGGCGC 20 4170

HSV2-UL30-4397 + CCCUUCUGGCCCGCAAGGCGC 21 12751

HSV2-UL30-4398 + GCCCU UCUGGCCCGCAAGGCGC 22 12752

HSV2-UL30-4399 + AGCCCUUCUGGCCCGCAAGGCGC 23 12753

HSV2-UL30-4400 + AAGCCCUUCUGGCCCGCAAGGCGC 24 12754

HSV2-UL30-4401 + CGAGGCACGGCCGGGCGC 18 12755

HSV2-UL30-4402 + CCGAGGCACGGCCGGGCGC 19 12756

HSV2-UL30-2485 + CCCGAGGCACGGCCGGGCGC 20 5220

HSV2-UL30-4403 + CCCCGAGGCACGGCCGGGCGC 21 12757

HSV2-UL30-4404 + CCCCCGAGGCACGGCCGGGCGC 22 12758

HSV2-UL30-4405 + CCCCCCGAGGCACGGCCGGGCGC 23 12759

HSV2-UL30-4406 + UCCCCCCGAGGCACGGCCGGGCGC 24 12760

HSV2-UL30-4407 + UCCCCGUAGAUGAUGCGC 18 12761

HSV2-UL30-4408 + GUCCCCGUAGAUGAUGCGC 19 12762

HSV2-UL30-2430 + UGUCCCCGUAGAUGAUGCGC 20 5182

HSV2-UL30-4409 + GUGUCCCCGUAGAUGAUGCGC 21 12763

HSV2-UL30-4410 + CGUGUCCCCGUAGAUGAUGCGC 22 12764

HSV2-UL30-4411 + CCGUGUCCCCGUAGAUGAUGCGC 23 12765

HSV2-UL30-4412 + UCCGUGUCCCCGUAGAUGAUGCGC 24 12766

HSV2-UL30-4413 + GGCGCUCGCAGAGAUCGC 18 12767

HSV2-UL30-4414 + AGGCGCUCGCAGAGAUCGC 19 12768

HSV2-UL30-2585 + CAGGCGCUCGCAGAGAUCGC 20 5295

HSV2-UL30-4415 + CCAGGCGCUCGCAGAGAUCGC 21 12769

HSV2-UL30-4416 + GCCAGGCGCUCGCAGAGAUCGC 22 12770

HSV2-UL30-4417 + CG CCAG G CG CU CG CAG AG AU CG C 23 12771

HSV2-UL30-4418 + CCGCCAGGCGCUCGCAGAGAUCGC 24 12772

HSV2-UL30-4419 + AGUUAAACUCGACGUCGC 18 12773

HSV2-UL30-4420 + CAGU UAAACUCGACGUCGC 19 12774

HSV2-UL30-2560 + GCAGUUAAACUCGACGUCGC 20 5276

HSV2-UL30-4421 + UGCAGUUAAACUCGACGUCGC 21 12775

HSV2-UL30-4422 + GUGCAGU UAAACUCGACGUCGC 22 12776

HSV2-UL30-4423 + CGUGCAGUUAAACUCGACGUCGC 23 12777

HSV2-UL30-4424 + CCGUGCAGUUAAACUCGACGUCGC 24 12778

HSV2-UL30-4425 + CACGUACGCGCGCGUCGC 18 12779

HSV2-UL30-4426 + GCACGUACGCGCGCGUCGC 19 12780

HSV2-UL30-2440 + UGCACGUACGCGCGCGUCGC 20 5188

HSV2-UL30-4427 + GUGCACGUACGCGCGCGUCGC 21 12781

HSV2-UL30-4428 + CGUGCACGUACGCGCGCGUCGC 22 12782

HSV2-UL30-4429 + GCGUGCACGUACGCGCGCGUCGC 23 12783

HSV2-UL30-4430 + CGCGUGCACGUACGCGCGCGUCGC 24 12784 HSV2-UL30-4431 + AGCGCGCGCCCGCUUCGC 18 12785

HSV2-UL30-4432 + CAGCGCGCGCCCGCU UCGC 19 12786

HSV2-UL30-2575 + CCAGCGCGCGCCCGCUUCGC 20 5287

HSV2-UL30-4433 + GCC AGCGCGCGCCCGCUUCGC 21 12787

HSV2-UL30-4434 + GGCCAGCGCGCGCCCGCUUCGC 22 12788

HSV2-UL30-4435 + AGGCCAGCGCGCGCCCGCUUCGC 23 12789

HSV2-UL30-4436 + UAGGCCAGCGCGCGCCCGCUUCGC 24 12790

HSV2-UL30-4437 + GCGCCGGCCCCCGCCGGC 18 12791

HSV2-UL30-4438 + AGCGCCGGCCCCCGCCGGC 19 12792

HSV2-UL30-2338 + UAGCGCCGGCCCCCGCCGGC 20 5124

HSV2-UL30-4439 + GUAGCGCCGGCCCCCGCCGGC 21 12793

HSV2-UL30-4440 + CGUAGCGCCGGCCCCCGCCGGC 22 12794

HSV2-UL30-4441 + CCGUAGCGCCGGCCCCCGCCGGC 23 12795

HSV2-UL30-4442 + GCCGUAGCGCCGGCCCCCGCCGGC 24 12796

HSV2-UL30-4443 + GCGUUCCCGCGGCCGGGC 18 12797

HSV2-UL30-4444 + CGCGU UCCCGCGGCCGGGC 19 12798

HSV2-UL30-2568 + GCGCGUUCCCGCGGCCGGGC 20 5281

HSV2-UL30-4445 + GGCGCGUUCCCGCGGCCGGGC 21 12799

HSV2-UL30-4446 + CGGCGCGU UCCCGCGGCCGGGC 22 12800

HSV2-UL30-4447 + CCGGCGCGUUCCCGCGGCCGGGC 23 12801

HSV2-UL30-4448 + GCCGGCGCGUUCCCGCGGCCGGGC 24 12802

HSV2-UL30-4449 + ACCCCGCGCUGCGCGGGC 18 12803

HSV2-UL30-4450 + CACCCCGCGCUGCGCGGGC 19 12804

HSV2-UL30-2503 + UCACCCCGCGCUGCGCGGGC 20 5236

HSV2-UL30-4451 + AUCACCCCGCGCUGCGCGGGC 21 12805

HSV2-UL30-4452 + GAUCACCCCGCGCUGCGCGGGC 22 12806

HSV2-UL30-4453 + CGAUCACCCCGCGCUGCGCGGGC 23 12807

HSV2-UL30-4454 + CCGAUCACCCCGCGCUGCGCGGGC 24 12808

HSV2-UL30-4455 + ACCAGCAGCU UGCGGGGC 18 12809

HSV2-UL30-4456 + CACCAGCAGCU UGCGGGGC 19 12810

HSV2-UL30-2360 + ACACCAGCAGCU UGCGGGGC 20 5138

HSV2-UL30-4457 + G ACACCAGCAGCU UGCGGGGC 21 12811

HSV2-UL30-4458 + GGACACCAGCAGCU UGCGGGGC 22 12812

HSV2-UL30-4459 + CGGACACCAGCAGCUUGCGGGGC 23 12813

HSV2-UL30-4460 + UCGG ACACCAGCAGCU UGCGGGGC 24 12814

HSV2-UL30-4461 + CUCCCGGGGGCGCUUGGC 18 12815

HSV2-UL30-4462 + UCUCCCGGGGGCGCUUGGC 19 12816

HSV2-UL30-1127 + GUCUCCCGGGGGCGCUUGGC 20 3997

HSV2-UL30-4463 + CGUCUCCCGGGGGCGCU UGGC 21 12817

HSV2-UL30-4464 + GCGUCUCCCGGGGGCGCUUGGC 22 12818

HSV2-UL30-4465 + GGCGUCUCCCGGGGGCGCUUGGC 23 12819

HSV2-UL30-4466 + CGGCGUCUCCCGGGGGCGCUUGGC 24 12820

HSV2-UL30-4467 + CCGAUCACCCCGCGCUGC 18 12821

HSV2-UL30-4468 + GCCGAUCACCCCGCGCUGC 19 12822

HSV2-UL30-2502 + CG CCG AU CACCCCGCG C U G C 20 5235

HSV2-UL30-4469 + UCGCCGAUCACCCCGCGCUGC 21 12823 HSV2-UL30-4470 + CUCGCCGAUCACCCCGCGCUGC 22 12824

HSV2-UL30-4471 + ACUCGCCGAUCACCCCGCGCUGC 23 12825

HSV2-UL30-4472 + UACUCGCCGAUCACCCCGCGCUGC 24 12826

HSV2-UL30-4473 + G CCG CCCCCAG C AG G U G C 18 12827

HSV2-UL30-4474 + GGCCGCCCCCAGCAGGUGC 19 12828

HSV2-UL30-2350 + AGGCCGCCCCCAGCAGG UGC 20 5131

HSV2-UL30-4475 + CAGGCCGCCCCCAGCAGGUGC 21 12829

HSV2-UL30-4476 + GCAGGCCGCCCCCAGCAGGUGC 22 12830

HSV2-UL30-4477 + CGCAGGCCGCCCCCAGCAGGUGC 23 12831

HSV2-UL30-4478 + ACGCAGGCCGCCCCCAGCAGGUGC 24 12832

HSV2-UL30-4479 + GAAAACAGGAGGAUGUGC 18 12833

HSV2-UL30-4480 + CGAAAACAGGAGGAUGUGC 19 12834

HSV2-UL30-2547 + GCGAAAACAGGAGGAUGUGC 20 5265

HSV2-UL30-4481 + AGCGAAAACAGGAGGAUGUGC 21 12835

HSV2-UL30-4482 + GAGCGAAAACAGGAGGAUGUGC 22 12836

HSV2-UL30-4483 + CGAGCGAAAACAGGAGGAUGUGC 23 12837

HSV2-UL30-4484 + CCGAGCGAAAACAGGAGGAUGUGC 24 12838

HSV2-UL30-4485 + CCCUCGUACU UCCUGAUC 18 12839

HSV2-UL30-4486 + CCCCUCGUACU UCCUGAUC 19 12840

HSV2-UL30-2573 + CCCCCUCGUACU UCCUGAUC 20 5286

HSV2-UL30-4487 + CCCCCCUCGUACUUCCUGAUC 21 12841

HSV2-UL30-4488 + GCCCCCCUCGUACUUCCUGAUC 22 12842

HSV2-UL30-4489 + CGCCCCCCUCGUACU UCCUGAUC 23 12843

HSV2-UL30-4490 + ACGCCCCCCUCGUACUUCCUGAUC 24 12844

HSV2-UL30-4491 + UCGCACUCGAGCUUGAUC 18 12845

HSV2-UL30-4492 + UUCGCACUCGAGCU UGAUC 19 12846

HSV2-UL30-1204 + UUUCGCACUCGAGCU UGAUC 20 4074

HSV2-UL30-4493 + UUUUCGCACUCGAGCUUGAUC 21 12847

HSV2-UL30-4494 + UUU UUCGCACUCGAGCUUGAUC 22 12848

HSV2-UL30-4495 + U UUUUUCGCACUCGAGCUUGAUC 23 12849

HSV2-UL30-4496 + GU UUUUUCGCACUCGAGCUUGAUC 24 12850

HSV2-UL30-4497 + GAAUUCGCUGUCAAACUC 18 12851

HSV2-UL30-4498 + CGAAUUCGCUGUCAAACUC 19 12852

HSV2-UL30-2527 + UCGAAU UCGCUGUCAAACUC 20 5253

HSV2-UL30-4499 + CUCGAAU UCGCUGUCAAACUC 21 12853

HSV2-UL30-4500 + UCUCGAAUUCGCUGUCAAACUC 22 12854

HSV2-UL30-4501 + AUCUCGAAUUCGCUGUCAAACUC 23 12855

HSV2-UL30-4502 + CAUCUCGAAU UCGCUGUCAAACUC 24 12856

HSV2-UL30-4503 + AUCGCUGAGGUGGGACUC 18 12857

HSV2-UL30-4504 + GAUCGCUGAGGUGGGACUC 19 12858

HSV2-UL30-1355 + AG AUCGCUGAGGUGGGACUC 20 4225

HSV2-UL30-4505 + GAG AUCGCUGAGGUGGGACUC 21 12859

HSV2-UL30-4506 + CG AG AUCGCUGAGGUGGGACUC 22 12860

HSV2-UL30-4507 + GCG AG AUCGCUGAGGUGGGACUC 23 12861

HSV2-UL30-4508 + GGCGAGAUCGCUGAGG UGGGACUC 24 12862

HSV2-UL30-4509 + GCGCAUGCCGGCCGCCUC 18 12863 HSV2-UL30-4510 + CGCGCAUGCCGGCCGCCUC 19 12864

HSV2-UL30-1223 + GCGCGCAUGCCGGCCGCCUC 20 4093

HSV2-UL30-4511 + GGCGCGCAUGCCGGCCGCCUC 21 12865

HSV2-UL30-4512 + GGGCGCGCAUGCCGGCCGCCUC 22 12866

HSV2-UL30-4513 + GGGGCGCGCAUGCCGGCCGCCUC 23 12867

HSV2-UL30-4514 + GGGGGCGCGCAUGCCGGCCGCCUC 24 12868

HSV2-UL30-4515 + GGGGCU UGGACGCGCCUC 18 12869

HSV2-UL30-4516 + CGGGGCUUGGACGCGCCUC 19 12870

HSV2-UL30-2361 + GCGGGGCUUGGACGCGCCUC 20 5139

HSV2-UL30-4517 + UGCGGGGCUUGGACGCGCCUC 21 12871

HSV2-UL30-4518 + U UGCGGGGCU UGGACGCGCCUC 22 12872

HSV2-UL30-4519 + CUUGCGGGGCUUGGACGCGCCUC 23 12873

HSV2-UL30-4520 + GCUUGCGGGGCU UGGACGCGCCUC 24 12874

HSV2-UL30-4521 + GGCGGCGGCGUCUAGCUC 18 12875

HSV2-UL30-4522 + GGGCGGCGGCGUCUAGCUC 19 12876

HSV2-UL30-2382 + GGGGCGGCGGCGUCUAGCUC 20 5147

HSV2-UL30-4523 + UGGGGCGGCGGCGUCUAGCUC 21 12877

HSV2-UL30-4524 + CUGGGGCGGCGGCGUCUAGCUC 22 12878

HSV2-UL30-4525 + CCUGGGGCGGCGGCGUCUAGCUC 23 12879

HSV2-UL30-4526 + CCCUGGGGCGGCGGCGUCUAGCUC 24 12880

HSV2-UL30-3064 + GGCGUCCAUAAAUCGCUC 18 11418

HSV2-UL30-3065 + UGGCGUCCAUAAAUCGCUC 19 11419

HSV2-UL30-2593 + AUGGCGUCCAUAAAUCGCUC 20 5300

HSV2-UL30-4527 + GAUGGCGUCCAUAAAUCGCUC 21 12881

HSV2-UL30-4528 + UGAUGGCGUCCAUAAAUCGCUC 22 12882

HSV2-UL30-4529 + GUGAUGGCGUCCAUAAAUCGCUC 23 12883

HSV2-UL30-4530 + CGUGAUGGCGUCCAUAAAUCGCUC 24 12884

HSV2-UL30-4531 + GCCUCCUCGGGGGUGCUC 18 12885

HSV2-UL30-4532 + GGCCUCCUCGGGGGUGCUC 19 12886

HSV2-UL30-1244 + CGGCCUCCUCGGGGGUGCUC 20 4114

HSV2-UL30-4533 + ACGGCCUCCUCGGGGGUGCUC 21 12887

HSV2-UL30-4534 + GACGGCCUCCUCGGGGGUGCUC 22 12888

HSV2-UL30-4535 + GGACGGCCUCCUCGGGGGUGCUC 23 12889

HSV2-UL30-4536 + AGGACGGCCUCCUCGGGGGUGCUC 24 12890

HSV2-UL30-4537 + AAACAGGAGGAUGUGCUC 18 12891

HSV2-UL30-4538 + AAAACAGGAGGAUGUGCUC 19 12892

HSV2-UL30-2548 + GAAAACAGGAGGAUGUGCUC 20 5266

HSV2-UL30-4539 + CG AAAACAGG AGG AUG UGCUC 21 12893

HSV2-UL30-4540 + GCG AAAACAGGAGGAUGUGCUC 22 12894

HSV2-UL30-4541 + AGCG AAAACAGGAGGAUGUGCUC 23 12895

HSV2-UL30-4542 + GAGCGAAAACAGGAGGAUGUGCUC 24 12896

HSV2-UL30-4543 + GGCAUGCGACGGCGUCUC 18 12897

HSV2-UL30-4544 + CGGCAUGCGACGGCGUCUC 19 12898

HSV2-UL30-2365 + UCGGCAUGCGACGGCGUCUC 20 5140

HSV2-UL30-4545 + GUCGGCAUGCGACGGCGUCUC 21 12899

HSV2-UL30-4546 + GGUCGGCAUGCGACGGCGUCUC 22 12900 HSV2-UL30-4547 + GGGUCGGCAUGCGACGGCGUCUC 23 12901

HSV2-UL30-4548 + GGGGUCGGCAUGCGACGGCGUCUC 24 12902

HSV2-UL30-4549 + UCCGGGGGGUGCCACGUC 18 12903

HSV2-UL30-4550 + GUCCGGGGGGUGCCACGUC 19 12904

HSV2-UL30-2344 + CGUCCGGGGGGUGCCACGUC 20 5127

HSV2-UL30-4551 + UCGUCCGGGGGGUGCCACGUC 21 12905

HSV2-UL30-4552 + GUCGUCCGGGGGGUGCCACGUC 22 12906

HSV2-UL30-4553 + CGUCGUCCGGGGGGUGCCACGUC 23 12907

HSV2-UL30-4554 + ACGUCGUCCGGGGGGUGCCACGUC 24 12908

HSV2-UL30-4555 + G AGG U U CCGAACG CCG U C 18 12909

HSV2-UL30-4556 + CGAGGUUCCGAACGCCGUC 19 12910

HSV2-UL30-1383 + UCGAGGUUCCGAACGCCGUC 20 4253

HSV2-UL30-4557 + CUCGAGGUUCCGAACGCCGUC 21 12911

HSV2-UL30-4558 + GCUCGAGGUUCCGAACGCCGUC 22 12912

HSV2-UL30-4559 + CGCUCGAGGUUCCGAACGCCGUC 23 12913

HSV2-UL30-4560 + UCGCUCGAGGU UCCGAACGCCGUC 24 12914

HSV2-UL30-4561 + UGCACGUACGCGCGCGUC 18 12915

HSV2-UL30-4562 + GUGCACGUACGCGCGCGUC 19 12916

HSV2-UL30-2439 + CGUGCACGUACGCGCGCGUC 20 5187

HSV2-UL30-4563 + GCGUGCACGUACGCGCGCGUC 21 12917

HSV2-UL30-4564 + CGCGUGCACGUACGCGCGCGUC 22 12918

HSV2-UL30-4565 + GCGCGUGCACGUACGCGCGCGUC 23 12919

HSV2-UL30-4566 + CGCGCGUGCACGUACGCGCGCGUC 24 12920

HSV2-UL30-4567 + CCGCGCGGCCACGUCGUC 18 12921

HSV2-UL30-4568 + GCCGCGCGGCCACGUCGUC 19 12922

HSV2-UL30-1091 + AGCCGCGCGGCCACGUCGUC 20 3961

HSV2-UL30-4569 + GAGCCGCGCGGCCACGUCGUC 21 12923

HSV2-UL30-4570 + UGAGCCGCGCGGCCACGUCGUC 22 12924

HSV2-UL30-4571 + CUGAGCCGCGCGGCCACGUCGUC 23 12925

HSV2-UL30-4572 + CCUGAGCCGCGCGGCCACGUCGUC 24 12926

HSV2-UL30-4573 + UACUGCUUGACGAAGGUC 18 12927

HSV2-UL30-4574 + GUACUGCU UGACGAAGGUC 19 12928

HSV2-UL30-2525 + CGUACUGCU UGACGAAGGUC 20 5251

HSV2-UL30-4575 + CCGUACUGCUUGACGAAGGUC 21 12929

HSV2-UL30-4576 + GCCGUACUGCUUGACGAAGGUC 22 12930

HSV2-UL30-4577 + GGCCG UACUGCUUGACGAAGGUC 23 12931

HSV2-UL30-4578 + GGGCCGUACUGCUUGACGAAGGUC 24 12932

HSV2-UL30-4579 + AUGAUGU UGUACCCGGUC 18 12933

HSV2-UL30-4580 + GAUGAUGUUGUACCCGGUC 19 12934

HSV2-UL30-2521 + UGAUGAUGUUGUACCCGGUC 20 5248

HSV2-UL30-4581 + UUGAUGAUGU UGUACCCGGUC 21 12935

HSV2-UL30-4582 + GU UGAUGAUGUUGUACCCGGUC 22 12936

HSV2-UL30-4583 + AGUUGAUGAUGUUGUACCCGGUC 23 12937

HSV2-UL30-4584 + AAGUUGAUGAUGUUGUACCCGGUC 24 12938

HSV2-UL30-4585 + AAACCCGGAGG UGGGGUC 18 12939

HSV2-UL30-4586 + GAAACCCGGAGGUGGGGUC 19 12940 HSV2-UL30-2481 + UGAAACCCGGAGGUGGGGUC 20 5216

HSV2-UL30-4587 + GUGAAACCCGGAGGUGGGGUC 21 12941

HSV2-UL30-4588 + CG UGAAACCCGGAGG UGGGGUC 22 12942

HSV2-UL30-4589 + ACGUGAAACCCGGAGGUGGGGUC 23 12943

HSV2-UL30-4590 + GACGUGAAACCCGGAGGUGGGGUC 24 12944

HSV2-UL30-4591 + GGCGGCCUGUUGCUUG UC 18 12945

HSV2-UL30-4592 + UGGCGGCCUGUUGCUUGUC 19 12946

HSV2-UL30-2447 + AUGGCGGCCUGUUGCU UGUC 20 5194

HSV2-UL30-4593 + GAUGGCGGCCUGUUGCUUGUC 21 12947

HSV2-UL30-4594 + UGAUGGCGGCCUGUUGCUUGUC 22 12948

HSV2-UL30-4595 + UUGAUGGCGGCCUGUUGCUUGUC 23 12949

HSV2-UL30-4596 + CUUGAUGGCGGCCUGU UGCU UGUC 24 12950

HSV2-UL30-4597 + UCGCGUACGUGGGCCU UC 18 12951

HSV2-UL30-4598 + CUCGCGUACGUGGGCCU UC 19 12952

HSV2-UL30-2461 + UCUCGCG UACGUGGGCCUUC 20 5202

HSV2-UL30-4599 + CUCUCGCGUACGUGGGCCUUC 21 12953

HSV2-UL30-4600 + GCUCUCGCGUACGUGGGCCUUC 22 12954

HSV2-UL30-4601 + GGCUCUCGCGUACGUGGGCCUUC 23 12955

HSV2-UL30-4602 + AGGCUCUCGCGUACGUGGGCCUUC 24 12956

HSV2-UL30-4603 + GGCUUGAGGCGG UACCAG 18 12957

HSV2-UL30-4604 + GGGCUUGAGGCGGUACCAG 19 12958

HSV2-UL30-2569 + CGGGCU UGAGGCGGUACCAG 20 5282

HSV2-UL30-4605 + CCGGGCUUGAGGCGG UACCAG 21 12959

HSV2-UL30-4606 + GCCGGGCUUGAGGCGGUACCAG 22 12960

HSV2-UL30-4607 + GGCCGGGCU UGAGGCGGUACCAG 23 12961

HSV2-UL30-4608 + CGGCCGGGCUUGAGGCGGUACCAG 24 12962

HSV2-UL30-4609 + GUCAGGACGAAGGGCCAG 18 12963

HSV2-UL30-4610 + GGUCAGGACGAAGGGCCAG 19 12964

HSV2-UL30-2520 + UGGUCAGGACGAAGGGCCAG 20 5247

HSV2-UL30-4611 + UUGGUCAGGACGAAGGGCCAG 21 12965

HSV2-UL30-4612 + CU UGGUCAGGACGAAGGGCCAG 22 12966

HSV2-UL30-4613 + GCU UGGUCAGGACGAAGGGCCAG 23 12967

HSV2-UL30-4614 + AGCUUGGUCAGGACGAAGGGCCAG 24 12968

HSV2-UL30-4615 + CUUGUAAUACACCGUCAG 18 12969

HSV2-UL30-4616 + GCUUGUAAUACACCGUCAG 19 12970

HSV2-UL30-2394 + AGCU UGUAAUACACCGUCAG 20 5156

HSV2-UL30-4617 + GAGCUUGUAAUACACCGUCAG 21 12971

HSV2-UL30-4618 + UGAGCUUGUAAUACACCGUCAG 22 12972

HSV2-UL30-4619 + AUGAGCUUGUAAUACACCGUCAG 23 12973

HSV2-UL30-4620 + CAUGAGCU UGUAAUACACCGUCAG 24 12974

HSV2-UL30-4621 + GCGCCCGUACCCGUCGAG 18 12975

HSV2-UL30-4622 + UGCGCCCGUACCCGUCGAG 19 12976

HSV2-UL30-1330 + AUGCGCCCGUACCCGUCGAG 20 4200

HSV2-UL30-4623 + CA U G CG CCCG U ACCCG U CG AG 21 12977

HSV2-UL30-4624 + UCAUGCGCCCGUACCCGUCGAG 22 12978

HSV2-UL30-4625 + U UCAUGCGCCCGUACCCGUCGAG 23 12979 HSV2-UL30-4626 + GUUCAUGCGCCCGUACCCGUCGAG 24 12980

HSV2-UL30-4627 + GGCCUGUUGCUUGUCGAG 18 12981

HSV2-UL30-4628 + CGGCCUGU UGCU UGUCGAG 19 12982

HSV2-UL30-2449 + GCGGCCUGUUGCU UGUCGAG 20 5195

HSV2-UL30-4629 + GGCGGCCUGUUGCU UGUCGAG 21 12983

HSV2-UL30-4630 + UGGCGGCCUGUUGCUUGUCGAG 22 12984

HSV2-UL30-4631 + AUGGCGGCCUGU UGCU UGUCGAG 23 12985

HSV2-UL30-4632 + GAUGGCGGCCUGUUGCUUGUCGAG 24

12986

HSV2-UL30-4633 + UCGACGUGAAACCCGGAG 18 12987

HSV2-UL30-4634 + GUCGACGUGAAACCCGGAG 19 12988

HSV2-UL30-2478 + GG UCGACGUGAAACCCGGAG 20 5214

HSV2-UL30-4635 + GGG UCGACGUGAAACCCGGAG 21 12989

HSV2-UL30-4636 + GGGG UCGACGUGAAACCCGGAG 22 12990

HSV2-UL30-4637 + CGGGG UCGACGUGAAACCCGGAG 23 12991

HSV2-UL30-4638 + ACGGGGUCGACGUGAAACCCGGAG 24 12992

HSV2-UL30-4639 + GCCGGCAGGCCCCUGGAG 18 12993

HSV2-UL30-4640 + GGCCGGCAGGCCCCUGGAG 19 12994

HSV2-UL30-2533 + GGGCCGGCAGGCCCCUGGAG 20 5256

HSV2-UL30-4641 + GGGGCCGGCAGGCCCCUGGAG 21 12995

HSV2-UL30-4642 + GGGGGCCGGCAGGCCCCUGGAG 22 12996

HSV2-UL30-4643 + CGGGGGCCGGCAGGCCCCUGGAG 23 12997

HSV2-UL30-4644 + ACGGGGGCCGGCAGGCCCCUGGAG 24 12998

HSV2-UL30-3084 + CAU AAAUCGCUCG UGG AG 18 11438

HSV2-UL30-3085 + CCAUAAAUCGCUCGUGGAG 19 11439

HSV2-UL30-3086 + UCCAUAAAUCGCUCGUGGAG 20 11440

HSV2-UL30-3087 + GUCCAU AAAUCGCUCG UGG AG 21 11441

HSV2-UL30-3088 + CGUCCAUAAAUCGCUCGUGGAG 22 11442

HSV2-UL30-3089 + GCG UCCAUAAAUCGCUCGUGGAG 23 11443

HSV2-UL30-3090 + GGCGUCCAUAAAUCGCUCGUGGAG 24 11444

HSV2-UL30-4645 + GGAGGCGAGAUCGCUGAG 18 12999

HSV2-UL30-4646 + UGGAGGCGAGAUCGCUGAG 19 13000

HSV2-UL30-2535 + CUGGAGGCGAGAUCGCUGAG 20 5258

HSV2-UL30-4647 + CCUGGAGGCGAGAUCGCUGAG 21 13001

HSV2-UL30-4648 + CCCUGGAGGCGAGAUCGCUGAG 22 13002

HSV2-UL30-4649 + CCCCUGGAGGCGAGAUCGCUGAG 23 13003

HSV2-UL30-4650 + GCCCCUGGAGGCGAGAUCGCUGAG 24 13004

HSV2-UL30-4651 + GUAAUAGUCCGUGUUGAG 18 13005

HSV2-UL30-4652 + AGUAAUAGUCCGUGUUGAG 19 13006

HSV2-UL30-1104 + AAGUAAUAGUCCGUGUUGAG 20 3974

HSV2-UL30-4653 + GAAGUAAUAGUCCGUG UUGAG 21 13007

HSV2-UL30-4654 + AGAAGUAAUAGUCCGUGUUGAG 22 13008

HSV2-UL30-4655 + GAGAAGUAAUAGUCCGUGUUGAG 23 13009

HSV2-UL30-4656 + CGAGAAGUAAUAGUCCGUGUUGAG 24 13010

HSV2-UL30-4657 + GGCCCGGAGGCGUAGUAG 18 13011

HSV2-UL30-4658 + GGGCCCGGAGGCGUAG UAG 19 13012 HSV2-UL30-2505 + CGGGCCCGGAGGCGUAGUAG 20 5237

HSV2-UL30-4659 + GCGGGCCCGGAGGCGUAGUAG 21 13013

HSV2-UL30-4660 + CGCGGGCCCGGAGGCGUAGUAG 22 13014

HSV2-UL30-4661 + GCGCGGGCCCGGAGGCGUAGUAG 23 13015

HSV2-UL30-4662 + UGCGCGGGCCCGGAGGCGUAGUAG 24 13016

HSV2-UL30-4663 + ACGGCGUUCAGCUUGUAG 18 13017

HSV2-UL30-4664 + GACGGCGUUCAGCU UGUAG 19 13018

HSV2-UL30-2508 + CGACGGCGU UCAGCUUG UAG 20 5238

HSV2-UL30-4665 + GCG ACGGCGUUCAGCU UGUAG 21 13019

HSV2-UL30-4666 + GGCG ACGGCGUUCAGCU UGUAG 22 13020

HSV2-UL30-4667 + CGGCG ACGGCG U UCAGCU UGU AG 23 13021

HSV2-UL30-4668 + UCGGCGACGGCGUUCAGCUUGUAG 24 13022

HSV2-UL30-4669 + UGGCCGAUGUCCCACACG 18 13023

HSV2-UL30-4670 + CUGGCCGAUGUCCCACACG 19 13024

HSV2-UL30-1328 + UCUGGCCGAUGUCCCACACG 20 4198

HSV2-UL30-4671 + CUCUGGCCGAUGUCCCACACG 21 13025

HSV2-UL30-4672 + GCUCUGGCCGAUGUCCCACACG 22 13026

HSV2-UL30-4673 + GGCUCUGGCCGAUGUCCCACACG 23 13027

HSV2-UL30-4674 + UGGCUCUGGCCGAUGUCCCACACG 24 13028

HSV2-UL30-4675 + CGUACGUGGGCCU UCACG 18 13029

HSV2-UL30-4676 + GCGUACGUGGGCCUUCACG 19 13030

HSV2-UL30-2462 + CGCGUACGUGGGCCUUCACG 20 5203

HSV2-UL30-4677 + UCGCGUACGUGGGCCU UCACG 21 13031

HSV2-UL30-4678 + CUCGCGUACGUGGGCCU UCACG 22 13032

HSV2-UL30-4679 + UCUCGCGUACGUGGGCCUUCACG 23 13033

HSV2-UL30-4680 + CUCUCGCGUACGUGGGCCUUCACG 24 13034

HSV2-UL30-4681 + CCCGCCAGGCGCGCGACG 18 13035

HSV2-UL30-4682 + GCCCGCCAGGCGCGCGACG 19 13036

HSV2-UL30-2496 + UGCCCGCCAGGCGCGCGACG 20 5230

HSV2-UL30-4683 + AUGCCCGCCAGGCGCGCGACG 21 13037

HSV2-UL30-4684 + GAUGCCCGCCAGGCGCGCGACG 22 13038

HSV2-UL30-4685 + UG AUGCCCGCCAGGCGCGCGACG 23 13039

HSV2-UL30-4686 + UUGAUGCCCGCCAGGCGCGCGACG 24 13040

HSV2-UL30-4687 + GGGACAUCAGCUUCGACG 18 13041

HSV2-UL30-4688 + CGGGACAUCAGCUUCGACG 19 13042

HSV2-UL30-2881 + GCGGGACAUCAGCUUCGACG 20 11235

HSV2-UL30-4689 + UGCGGGACAUCAGCUUCGACG 21 13043

HSV2-UL30-4690 + AUGCGGGACAUCAGCUUCGACG 22 13044

HSV2-UL30-4691 + GAUGCGGG ACAUCAGCU UCG ACG 23 13045

HSV2-UL30-4692 + AGAUGCGGGACAUCAGCUUCGACG 24 13046

HSV2-UL30-4693 + CUGUCAAACUCCAGGACG 18 13047

HSV2-UL30-4694 + GCUGUCAAACUCCAGGACG 19 13048

HSV2-UL30-2528 + CGCUGUCAAACUCCAGGACG 20 5254

HSV2-UL30-4695 + UCGCUGUCAAACUCCAGGACG 21 13049

HSV2-UL30-4696 + UUCGCUGUCAAACUCCAGGACG 22 13050

HSV2-UL30-4697 + AU UCGCUGUCAAACUCCAGGACG 23 13051 HSV2-UL30-4698 + AAU UCGCUGUCAAACUCCAGGACG 24 13052

HSV2-UL30-4699 + GUCAGCUUGGUCAGGACG 18 13053

HSV2-UL30-4700 + CGUCAGCU UGGUCAGGACG 19 13054

HSV2-UL30-2519 + CCGUCAGCUUGGUCAGGACG 20 5246

HSV2-UL30-4701 + UCCGUCAGCUUGGUCAGGACG 21 13055

HSV2-UL30-4702 + CUCCGUCAGCU UGGUCAGGACG 22 13056

HSV2-UL30-4703 + UCUCCGUCAGCUUGGUCAGGACG 23 13057

HSV2-UL30-4704 + AUCUCCGUCAGCUUGGUCAGGACG 24 13058

HSV2-UL30-4705 + UUCAUGCGCCCGUACCCG 18 13059

HSV2-UL30-4706 + GUUCAUGCGCCCGUACCCG 19 13060

HSV2-UL30-2514 + CGU UCAUGCGCCCGUACCCG 20 5242

HSV2-UL30-4707 + CCGUUCAUGCGCCCGUACCCG 21 13061

HSV2-UL30-4708 + GCCGUUCAUGCGCCCGUACCCG 22 13062

HSV2-UL30-4709 + GGCCGUUCAUGCGCCCGUACCCG 23 13063

HSV2-UL30-4710 + CGGCCG U UCAUGCGCCCGUACCCG 24 13064

HSV2-UL30-4711 + AGACCGUGCUGCACCCCG 18 13065

HSV2-UL30-4712 + AAGACCGUGCUGCACCCCG 19 13066

HSV2-UL30-2444 + G A AG ACCG U G CU G CACCCCG 20 5191

HSV2-UL30-4713 + AGAAGACCG UGCUGCACCCCG 21 13067

HSV2-UL30-4714 + CAGAAGACCGUGCUGCACCCCG 22 13068

HSV2-UL30-4715 + GCAG AAGACCGUGCUGCACCCCG 23 13069

HSV2-UL30-4716 + GGCAG AAGACCGUGCUGCACCCCG 24 13070

HSV2-UL30-4717 + AGCGUGAUGACGGUCCCG 18 13071

HSV2-UL30-4718 + AAGCGUGAUGACGGUCCCG 19 13072

HSV2-UL30-2592 + GAAGCGUGAUGACGGUCCCG 20 5299

HSV2-UL30-4719 + AGAAGCGUGAUGACGGUCCCG 21 13073

HSV2-UL30-4720 + CAG AAGCGUGAUGACGGUCCCG 22 13074

HSV2-UL30-4721 + CCAG AAGCGUGAUGACGGUCCCG 23 13075

HSV2-UL30-4722 + CCCAGAAGCGUGAUGACGGUCCCG 24 13076

HSV2-UL30-4723 + UCGAGGUUCCGAACGCCG 18 13077

HSV2-UL30-4724 + CUCGAGGUUCCGAACGCCG 19 13078

HSV2-UL30-2562 + GCUCGAGGUUCCGAACGCCG 20 5278

HSV2-UL30-4725 + CGCUCGAGGUUCCGAACGCCG 21 13079

HSV2-UL30-4726 + UCGCUCGAGGUUCCGAACGCCG 22 13080

HSV2-UL30-4727 + GUCGCUCGAGGU UCCGAACGCCG 23 13081

HSV2-UL30-4728 + CGUCGCUCGAGGU UCCGAACGCCG 24 13082

HSV2-UL30-4729 + CGGGUCGGUGAUGCGCCG 18 13083

HSV2-UL30-4730 + CCGGGUCGGUGAUGCGCCG 19 13084

HSV2-UL30-2400 + UCCGGGUCGGUGAUGCGCCG 20 5162

HSV2-UL30-4731 + CUCCGGGUCGGUGAUGCGCCG 21 13085

HSV2-UL30-4732 + UCUCCGGGUCGGUGAUGCGCCG 22 13086

HSV2-UL30-4733 + CUCUCCGGGUCGGUGAUGCGCCG 23 13087

HSV2-UL30-4734 + CCUCUCCGGGUCGGUGAUGCGCCG 24 13088

HSV2-UL30-4735 + CCGGGGAGGGCAGGGCCG 18 13089

HSV2-UL30-4736 + GCCGGGGAGGGCAGGGCCG 19 13090

HSV2-UL30-2374 + GGCCGGGGAGGGCAGGGCCG 20 5144 HSV2-UL30-4737 + UGGCCGGGGAGGGCAGGGCCG 21 13091

HSV2-UL30-4738 + UUGGCCGGGGAGGGCAGGGCCG 22 13092

HSV2-UL30-4739 + CU UGGCCGGGGAGGGCAGGGCCG 23 13093

HSV2-UL30-4740 + GCUUGGCCGGGGAGGGCAGGGCCG 24 13094

HSV2-UL30-4741 + CGCGACGGCCUCGGGCCG 18 13095

HSV2-UL30-4742 + GCGCGACGGCCUCGGGCCG 19 13096

HSV2-UL30-2464 + UGCGCGACGGCCUCGGGCCG 20 5205

HSV2-UL30-4743 + GUGCGCGACGGCCUCGGGCCG 21 13097

HSV2-UL30-4744 + GG UGCGCGACGGCCUCGGGCCG 22 13098

HSV2-UL30-4745 + AGG UGCGCGACGGCCUCGGGCCG 23 13099

HSV2-UL30-4746 + CAGG UGCGCGACGGCCUCGGGCCG 24 13100

HSV2-UL30-4747 + CCCGGGGGCGCU UGGCCG 18 13101

HSV2-UL30-4748 + UCCCGGGGGCGCUUGGCCG 19 13102

HSV2-UL30-1129 + CUCCCGGGGGCGCU UGGCCG 20 3999

HSV2-UL30-4749 + UCUCCCGGGGGCGCUUGGCCG 21 13103

HSV2-UL30-4750 + GUCUCCCGGGGGCGCU UGGCCG 22 13104

HSV2-UL30-4751 + CGUCUCCCGGGGGCGCU UGGCCG 23 13105

HSV2-UL30-4752 + GCGUCUCCCGGGGGCGCUUGGCCG 24 13106

HSV2-UL30-4753 + UGGUCCGCGGAGAUGCCG 18 13107

HSV2-UL30-4754 + GUGGUCCGCGGAGAUGCCG 19 13108

HSV2-UL30-1417 + AG UGGUCCGCGGAGAUGCCG 20 4287

HSV2-UL30-4755 + AAG UGGUCCGCGGAGAUGCCG 21 13109

HSV2-UL30-4756 + GAAG UGGUCCGCGGAGAUGCCG 22 13110

HSV2-UL30-4757 + CG AAG UGGUCCGCGGAGAUGCCG 23 13111

HSV2-UL30-4758 + UCGAAGUGGUCCGCGGAGAUGCCG 24 13112

HSV2-UL30-4759 + CCGCCUCGAAGUGGUCCG 18 13113

HSV2-UL30-4760 + UCCGCCUCGAAGUGGUCCG 19 13114

HSV2-UL30-1416 + CUCCGCCUCGAAGUGGUCCG 20 4286

HSV2-UL30-4761 + CCUCCGCCUCGAAGUGG UCCG 21 13115

HSV2-UL30-4762 + ACCUCCGCCUCGAAGUGGUCCG 22 13116

HSV2-UL30-4763 + CACCU CCGCCU CG AAG U GG UCCG 23 13117

HSV2-UL30-4764 + CCACCUCCGCCUCGAAGUGGUCCG 24 13118

HSV2-UL30-4765 + GGACGGGACCUGCGCGCG 18 13119

HSV2-UL30-4766 + UGGACGGGACCUGCGCGCG 19 13120

HSV2-UL30-2392 + AUGGACGGGACCUGCGCGCG 20 5154

HSV2-UL30-4767 + GAUGGACGGGACCUGCGCGCG 21 13121

HSV2-UL30-4768 + UG AUGGACGGGACCUGCGCGCG 22 13122

HSV2-UL30-4769 + UUGAUGGACGGGACCUGCGCGCG 23 13123

HSV2-UL30-4770 + CU UGAUGGACGGGACCUGCGCGCG 24 13124

HSV2-UL30-4771 + GCGCUCGCAGAGAUCGCG 18 13125

HSV2-UL30-4772 + GGCGCUCGCAGAGAUCGCG 19 13126

HSV2-UL30-1422 + AGGCGCUCGCAGAGAUCGCG 20 4292

HSV2-UL30-4773 + CAGGCGCUCGCAGAGAUCGCG 21 13127

HSV2-UL30-4774 + CCAGGCGCUCGCAGAGAUCGCG 22 13128

HSV2-UL30-4775 + GCCAGGCGCUCGCAGAGAUCGCG 23 13129

HSV2-UL30-4776 + CGCCAGGCGCUCGCAGAGAUCGCG 24 13130 HSV2-UL30-4777 + CGCCCCCGGCGACUCGCG 18 13131

HSV2-UL30-4778 + ACGCCCCCGGCGACUCGCG 19 13132

HSV2-UL30-2583 + GACG CCCCCGG CG ACU CGCG 20 5293

HSV2-UL30-4779 + CGACGCCCCCGGCGACUCGCG 21 13133

HSV2-UL30-4780 + ACGACGCCCCCGGCGACUCGCG 22 13134

HSV2-UL30-4781 + AACGACGCCCCCGGCGACUCGCG 23 13135

HSV2-UL30-4782 + GAACGACGCCCCCGGCGACUCGCG 24 13136

HSV2-UL30-4783 + GGCGGCG UCUAGCUCGCG 18 13137

HSV2-UL30-4784 + CGGCGGCGUCUAGCUCGCG 19 13138

HSV2-UL30-2384 + GCGGCGGCGUCUAGCUCGCG 20 5148

HSV2-UL30-4785 + GGCGGCGGCGUCUAGCUCGCG 21 13139

HSV2-UL30-4786 + GGGCGGCGGCGUCUAGCUCGCG 22 13140

HSV2-UL30-4787 + GGGGCGGCGGCGUCUAGCUCGCG 23 13141

HSV2-UL30-4788 + UGGGGCGGCGGCGUCUAGCUCGCG 24 13142

HSV2-UL30-4789 + ACGUACGCGCGCGUCGCG 18 13143

HSV2-UL30-4790 + CACGUACGCGCGCGUCGCG 19 13144

HSV2-UL30-1225 + GCACGUACGCGCGCGUCGCG 20 4095

HSV2-UL30-4791 + UGCACGUACGCGCGCGUCGCG 21 13145

HSV2-UL30-4792 + GUGCACGUACGCGCGCGUCGCG 22 13146

HSV2-UL30-4793 + CGUGCACGUACGCGCGCGUCGCG 23 13147

HSV2-UL30-4794 + GCGUGCACGUACGCGCGCGUCGCG 24 13148

HSV2-UL30-4795 + CU UCUGGCCCGCAAGGCG 18 13149

HSV2-UL30-4796 + CCUUCUGGCCCGCAAGGCG 19 13150

HSV2-UL30-2491 + CCCUUCUGGCCCGCAAGGCG 20 5226

HSV2-UL30-4797 + GCCCUUCUGGCCCGCAAGGCG 21 13151

HSV2-UL30-4798 + AGCCCUUCUGGCCCGCAAGGCG 22 13152

HSV2-UL30-4799 + AAGCCCUUCUGGCCCGCAAGGCG 23 13153

HSV2-UL30-4800 + GAAGCCCUUCUGGCCCGCAAGGCG 24 13154

HSV2-UL30-4801 + UGUCUGCUCAGUUCGGCG 18 13155

HSV2-UL30-4802 + GUGUCUGCUCAGUUCGGCG 19 13156

HSV2-UL30-2396 + GGUGUCUGCUCAGUUCGGCG 20 5158

HSV2-UL30-4803 + GGGUGUCUGCUCAGUUCGGCG 21 13157

HSV2-UL30-4804 + CGGGUGUCUGCUCAG UUCGGCG 22 13158

HSV2-UL30-4805 + GCGGGUGUCUGCUCAGUUCGGCG 23 13159

HSV2-UL30-4806 + CGCGGGUGUCUGCUCAGU UCGGCG 24 13160

HSV2-UL30-4807 + AUGUACUUUUUCUUGGCG 18 13161

HSV2-UL30-4808 + GAUGUACUU UUUCU UGGCG 19 13162

HSV2-UL30-2414 + CGAUGUACUUUU UCUUGGCG 20 5173

HSV2-UL30-4809 + CCGAUGUACUU UUUCU UGGCG 21 13163

HSV2-UL30-4810 + GCCGAUGUACU UUUUCU UGGCG 22 13164

HSV2-UL30-4811 + CGCCGAUGUACU UUUUCUUGGCG 23 13165

HSV2-UL30-4812 + ACGCCGAUGUACUUUU UCUUGGCG 24 13166

HSV2-UL30-4813 + CCCCUGGAGGCGAGAUCG 18 13167

HSV2-UL30-4814 + GCCCCUGGAGGCGAGAUCG 19 13168

HSV2-UL30-2534 + GGCCCCUGGAGGCGAGAUCG 20 5257

HSV2-UL30-4815 + AGGCCCCUGGAGGCGAGAUCG 21 13169 HSV2-UL30-4816 + CAGGCCCCUGGAGGCGAGAUCG 22 13170

HSV2-UL30-4817 + GCAGGCCCCUGGAGGCGAGAUCG 23 13171

HSV2-UL30-4818 + GGCAGGCCCCUGGAGGCGAGAUCG 24 13172

HSV2-UL30-4819 + CCUCGUACUUCCUGAUCG 18 13173

HSV2-UL30-4820 + CCCUCGUACUUCCUGAUCG 19 13174

HSV2-UL30-1404 + CCCCUCGUACU UCCUGAUCG 20 4274

HSV2-UL30-4821 + CCCCCUCGUACUUCCUGAUCG 21 13175

HSV2-UL30-4822 + CCCCCCUCGUACUUCCUGAUCG 22 13176

HSV2-UL30-4823 + GCCCCCCUCGUACUUCCUGAUCG 23 13177

HSV2-UL30-4824 + CGCCCCCCUCGUACU UCCUGAUCG 24 13178

HSV2-UL30-4825 + CGCACUCGAGCUUGAUCG 18 13179

HSV2-UL30-4826 + UCGCACUCGAGCUUGAUCG 19 13180

HSV2-UL30-1205 + UUCGCACUCGAGCUUGAUCG 20 4075

HSV2-UL30-4827 + UUUCGCACUCGAGCU UGAUCG 21 13181

HSV2-UL30-4828 + U UUUCGCACUCGAGCUUGAUCG 22 13182

HSV2-UL30-4829 + UUUUUCGCACUCGAGCU UGAUCG 23 13183

HSV2-UL30-4830 + UUUUUUCGCACUCGAGCUUGAUCG 24 13184

HSV2-UL30-4831 + UCGCUGAGGUGGGACUCG 18 13185

HSV2-UL30-4832 + AUCGCUGAGGUGGGACUCG 19 13186

HSV2-UL30-1356 + GAUCGCUGAGGUGGGACUCG 20 4226

HSV2-UL30-4833 + AG AUCGCUGAGGUGGGACUCG 21 13187

HSV2-UL30-4834 + GAG AUCGCUGAGGUGGGACUCG 22 13188

HSV2-UL30-4835 + CG AG AUCGCUGAGGUGGGACUCG 23 13189

HSV2-UL30-4836 + GCGAGAUCGCUGAGGUGGGACUCG 24 13190

HSV2-UL30-4837 + GCGGCGGCGUCUAGCUCG 18 13191

HSV2-UL30-4838 + GGCGGCGGCGUCUAGCUCG 19 13192

HSV2-UL30-1147 + GGGCGGCGGCGUCUAGCUCG 20 4017

HSV2-UL30-4839 + GGGGCGGCGGCGUCUAGCUCG 21 13193

HSV2-UL30-4840 + UGGGGCGGCGGCGUCUAGCUCG 22 13194

HSV2-UL30-4841 + CUGGGGCGGCGGCGUCUAGCUCG 23 13195

HSV2-UL30-4842 + CCUGGGGCGGCGGCGUCUAGCUCG 24 13196

HSV2-UL30-4843 + GCCGCCGCCAGGCGCUCG 18 13197

HSV2-UL30-4844 + GGCCGCCGCCAGGCGCUCG 19 13198

HSV2-UL30-2584 + GGGCCGCCGCCAGGCGCUCG 20 5294

HSV2-UL30-4845 + AGGGCCGCCGCCAGGCGCUCG 21 13199

HSV2-UL30-4846 + CAGGGCCGCCGCCAGGCGCUCG 22 13200

HSV2-UL30-4847 + GCAGGGCCGCCGCCAGGCGCUCG 23 13201

HSV2-UL30-4848 + CGCAGGGCCGCCGCCAGGCGCUCG 24 13202

HSV2-UL30-3145 + GCGUCCAUAAAUCGCUCG 18 11499

HSV2-UL30-3146 + GGCGUCCAUAAAUCGCUCG 19 11500

HSV2-UL30-1438 + UGGCGUCCAUAAAUCGCUCG 20 4308

HSV2-UL30-3147 + AUGGCGUCCAUAAAUCGCUCG 21 11501

HSV2-UL30-3148 + GAUGGCGUCCAUAAAUCGCUCG 22 11502

HSV2-UL30-3149 + UGAUGGCGUCCAUAAAUCGCUCG 23 11503

HSV2-UL30-3150 + GUGAUGGCGUCCAUAAAUCGCUCG 24 11504

HSV2-UL30-4849 + AGGUUCCGAACGCCGUCG 18 13203 HSV2-UL30-4850 + G AG G U U CCG AACG CCG U CG 19 13204

HSV2-UL30-1384 + CGAGGUUCCGAACGCCGUCG 20 4254

HSV2-UL30-4851 + UCGAGGU UCCGAACGCCGUCG 21 13205

HSV2-UL30-4852 + CUCGAGGU UCCGAACGCCGUCG 22 13206

HSV2-UL30-4853 + GCUCGAGGUUCCGAACGCCGUCG 23 13207

HSV2-UL30-4854 + CGCUCGAGGUUCCGAACGCCGUCG 24 13208

HSV2-UL30-4855 + GCGGUGGUGGACAGGUCG 18 13209

HSV2-UL30-4856 + GGCGGUGGUGGACAGGUCG 19 13210

HSV2-UL30-2550 + GGGCGGUGGUGGACAGGUCG 20 5268

HSV2-UL30-4857 + AGGGCGGUGGUGGACAGGUCG 21 13211

HSV2-UL30-4858 + GAGGGCGGUGGUGGACAGGUCG 22 13212

HSV2-UL30-4859 + CGAGGGCGGUGG UGGACAGG UCG 23 13213

HSV2-UL30-4860 + UCGAGGGCGGUGGUGGACAGGUCG 24

13214

HSV2-UL30-4861 + GCGGCCUGUUGCU UGUCG 18 13215

HSV2-UL30-4862 + GGCGGCCUGUUGCUUG UCG 19 13216

HSV2-UL30-1237 + UGGCGGCCUGUUGCUUGUCG 20 4107

HSV2-UL30-4863 + AUGGCGGCCUGUUGCUUGUCG 21 13217

HSV2-UL30-4864 + GAUGGCGGCCUG UUGCUUGUCG 22 13218

HSV2-UL30-4865 + UGAUGGCGGCCUGUUGCUUGUCG 23 13219

HSV2-UL30-4866 + UUGAUGGCGGCCUGUUGCUUGUCG 24

13220

HSV2-UL30-4867 + UGCGGGACAUCAGCUUCG 18 13221

HSV2-UL30-4868 + AUGCGGGACAUCAGCUUCG 19 13222

HSV2-UL30-4869 + GAUGCGGGACAUCAGCU UCG 20 13223

HSV2-UL30-4870 + ACGGCCUCGGGCCGCAGG 18 13224

HSV2-UL30-4871 + GACGGCCUCGGGCCGCAGG 19 13225

HSV2-UL30-2467 + CG ACGGCCUCGGGCCGCAGG 20 5206

HSV2-UL30-4872 + GCG ACGGCCUCGGGCCGCAGG 21 13226

HSV2-UL30-4873 + CGCG ACGGCCUCGGGCCGCAGG 22 13227

HSV2-UL30-4874 + GCGCG ACGGCCUCGGGCCGCAGG 23 13228

HSV2-UL30-4875 + UGCGCGACGGCCUCGGGCCGCAGG 24 13229

HSV2-UL30-4876 + CCCUGGG UGUCCGGCAGG 18 13230

HSV2-UL30-4877 + CCCCUGGGUGUCCGGCAGG 19 13231

HSV2-UL30-2490 + GCCCCUGGGUGUCCGGCAGG 20 5225

HSV2-UL30-4878 + CGCCCCUGGGUGUCCGGCAGG 21 13232

HSV2-UL30-4879 + CCGCCCCUGGG UGUCCGGCAGG 22 13233

HSV2-UL30-4880 + ACCGCCCCUGGGUGUCCGGCAGG 23 13234

HSV2-UL30-4881 + AACCGCCCCUGGGUGUCCGGCAGG 24 13235

HSV2-UL30-4882 + GUGGCGGCCACGUGCAGG 18 13236

HSV2-UL30-4883 + GGUGGCGGCCACGUGCAGG 19 13237

HSV2-UL30-2442 + CGGUGGCGGCCACGUGCAGG 20 5189

HSV2-UL30-4884 + ACGGUGGCGGCCACGUGCAGG 21 13238

HSV2-UL30-4885 + CACGGUGGCGGCCACGUGCAGG 22 13239

HSV2-UL30-4886 + UCACGGUGGCGGCCACGUGCAGG 23 13240

HSV2-UL30-4887 + GUCACGGUGGCGGCCACGUGCAGG 24 13241 HSV2-UL30-4888 + UCCGUCAGCUUGGUCAGG 18 13242

HSV2-UL30-4889 + CUCCGUCAGCUUGGUCAGG 19 13243

HSV2-UL30-2518 + UCUCCGUCAGCUUGGUCAGG 20 5245

HSV2-UL30-4890 + AUCUCCGUCAGCUUGGUCAGG 21 13244

HSV2-UL30-4891 + GAUCUCCGUCAGCUUGGUCAGG 22 13245

HSV2-UL30-4892 + AGAUCUCCGUCAGCUUGGUCAGG 23 13246

HSV2-UL30-4893 + UAGAUCUCCGUCAGCU UGGUCAGG 24 13247

HSV2-UL30-4894 + GAGGCGAGAUCGCUGAGG 18 13248

HSV2-UL30-4895 + GGAGGCGAGAUCGCUGAGG 19 13249

HSV2-UL30-1352 + UGGAGGCGAGAUCGCUGAGG 20 4222

HSV2-UL30-4896 + CUGGAGGCGAGAUCGCUGAGG 21 13250

HSV2-UL30-4897 + CCUGGAGGCGAGAUCGCUGAGG 22 13251

HSV2-UL30-4898 + CCCUGGAGGCGAGAUCGCUGAGG 23 13252

HSV2-UL30-4899 + CCCCUGGAGGCGAGAUCGCUGAGG 24 13253

HSV2-UL30-4900 + GCCCGGAGGCGUAGUAGG 18 13254

HSV2-UL30-4901 + GGCCCGGAGGCGUAGUAGG 19 13255

HSV2-UL30-1317 + GGGCCCGGAGGCGUAGUAGG 20 4187

HSV2-UL30-4902 + CGGGCCCGGAGGCGUAGUAGG 21 13256

HSV2-UL30-4903 + GCGGGCCCGGAGGCGUAGUAGG 22 13257

HSV2-UL30-4904 + CGCGGGCCCGGAGGCGUAGUAGG 23 13258

HSV2-UL30-4905 + GCGCGGGCCCGGAGGCGUAGUAGG 24 13259

HSV2-UL30-4906 + CU UCCCCCCG AGGCACGG 18 13260

HSV2-UL30-4907 + CCU U CCCCCCG AG GCACGG 19 13261

HSV2-UL30-2484 + CCCU UCCCCCCG AGGCACGG 20 5219

HSV2-UL30-4908 + CCCCU UCCCCCCG AGGCACGG 21 13262

HSV2-UL30-4909 + U CCCC U U CCCCCCG AG G CACG G 22 13263

HSV2-UL30-4910 + CUCCCCUUCCCCCCGAGGCACGG 23 13264

HSV2-UL30-4911 + GCUCCCCUUCCCCCCGAGGCACGG 24 13265

HSV2-UL30-4912 + CCGCCAGGCGCGCGACGG 18 13266

HSV2-UL30-4913 + CCCGCCAGGCGCGCGACGG 19 13267

HSV2-UL30-1309 + GCCCGCCAGGCGCGCGACGG 20 4179

HSV2-UL30-4914 + UGCCCGCCAGGCGCGCGACGG 21 13268

HSV2-UL30-4915 + AUGCCCGCCAGGCGCGCGACGG 22 13269

HSV2-UL30-4916 + GAUGCCCGCCAGGCGCGCGACGG 23 13270

HSV2-UL30-4917 + UG AUGCCCGCCAGGCGCGCGACGG 24 13271

HSV2-UL30-4918 + GCCGGCGCGUUCCCGCGG 18 13272

HSV2-UL30-4919 + GGCCGGCGCGUUCCCGCGG 19 13273

HSV2-UL30-2567 + GGGCCGGCGCGU UCCCGCGG 20 5280

HSV2-UL30-4920 + UGGGCCGGCGCGUUCCCGCGG 21 13274

HSV2-UL30-4921 + UUGGGCCGGCGCGUUCCCGCGG 22 13275

HSV2-UL30-4922 + GUUGGGCCGGCGCG UUCCCGCGG 23 13276

HSV2-UL30-4923 + GGUUGGGCCGGCGCGU UCCCGCGG 24 13277

HSV2-UL30-4924 + CGCCGUCGGGGGGCGCGG 18 13278

HSV2-UL30-4925 + ACGCCGUCGGGGGGCGCGG 19 13279

HSV2-UL30-2566 + AACGCCGUCGGGGGGCGCGG 20 5279

HSV2-UL30-4926 + GAACGCCGUCGGGGGGCGCGG 21 13280 HSV2-UL30-4927 + CGAACGCCGUCGGGGGGCGCGG 22 13281

HSV2-UL30-4928 + CCGAACGCCGUCGGGGGGCGCGG 23 13282

HSV2-UL30-4929 + UCCGAACGCCGUCGGGGGGCGCGG 24 13283

HSV2-UL30-4930 + GGAAAGCUCCAGGUGCGG 18 13284

HSV2-UL30-4931 + CGGAAAGCUCCAGGUGCGG 19 13285

HSV2-UL30-2498 + GCGGAAAGCUCCAGGUGCGG 20 5231

HSV2-UL30-4932 + GGCGGAAAGCUCCAGGUGCGG 21 13286

HSV2-UL30-4933 + CGGCGGAAAGCUCCAGGUGCGG 22 13287

HSV2-UL30-4934 + ACGGCGGAAAGCUCCAGGUGCGG 23 13288

HSV2-UL30-4935 + GACGGCGGAAAGCUCCAGGUGCGG 24 13289

HSV2-UL30-4936 + GCACUCGAGCUUGAUCGG 18 13290

HSV2-UL30-4937 + CGCACUCGAGCUUGAUCGG 19 13291

HSV2-UL30-1206 + UCGCACUCGAGCUUGAUCGG 20 4076

HSV2-UL30-4938 + U UCGCACUCGAGCUUGAUCGG 21 13292

HSV2-UL30-4939 + U UUCGCACUCGAGCU UGAUCGG 22 13293

HSV2-UL30-4940 + UUUUCGCACUCGAGCUUGAUCGG 23 13294

HSV2-UL30-4941 + UUUUUCGCACUCGAGCU UGAUCGG 24 13295

HSV2-UL30-4942 + AGACGCGGUAGUACAGGG 18 13296

HSV2-UL30-4943 + AAGACGCGGUAGUACAGGG 19 13297

HSV2-UL30-2577 + GAAGACGCGGUAGUACAGGG 20 5289

HSV2-UL30-4944 + CG AAGACGCGGUAGUACAGGG 21 13298

HSV2-UL30-4945 + ACG AAGACGCGGUAGUACAGGG 22 13299

HSV2-UL30-4946 + CACGAAGACGCGGUAGUACAGGG 23 13300

HSV2-UL30-4947 + GCACG AAGACGCGGUAGUACAGGG 24 13301

HSV2-UL30-4948 + GGCCACGUGCAGGCAGGG 18 13302

HSV2-UL30-4949 + CGGCCACG UGCAGGCAGGG 19 13303

HSV2-UL30-2443 + GCGGCCACGUGCAGGCAGGG 20 5190

HSV2-UL30-4950 + GGCGGCCACG UGCAGGCAGGG 21 13304

HSV2-UL30-4951 + UGGCGGCCACGUGCAGGCAGGG 22 13305

HSV2-UL30-4952 + GUGGCGGCCACGUGCAGGCAGGG 23 13306

HSV2-UL30-4953 + GGUGGCGGCCACGUGCAGGCAGGG 24 13307

HSV2-UL30-4954 + GCGCUUGGCCGGGGAGGG 18 13308

HSV2-UL30-4955 + GGCGCU UGGCCGGGGAGGG 19 13309

HSV2-UL30-2373 + GGGCGCUUGGCCGGGGAGGG 20 5143

HSV2-UL30-4956 + GGGGCGCUUGGCCGGGGAGGG 21 13310

HSV2-UL30-4957 + GGGGGCGCU UGGCCGGGGAGGG 22 13311

HSV2-UL30-4958 + CGGGGGCGCUUGGCCGGGGAGGG 23 13312

HSV2-UL30-4959 + CCGGGGGCGCU UGGCCGGGGAGGG 24 13313

HSV2-UL30-4960 + CGGGGGCGCUUGGCCGGG 18 13314

HSV2-UL30-4961 + CCGGGGGCGCUUGGCCGGG 19 13315

HSV2-UL30-2372 + CCCGGGGGCGCUUGGCCGGG 20 5142

HSV2-UL30-4962 + UCCCGGGGGCGCU UGGCCGGG 21 13316

HSV2-UL30-4963 + CUCCCGGGGGCGCUUGGCCGGG 22 13317

HSV2-UL30-4964 + UCUCCCGGGGGCGCUUGGCCGGG 23 13318

HSV2-UL30-4965 + GUCUCCCGGGGGCGCUUGGCCGGG 24 13319

HSV2-UL30-4966 + CACUCGAGCUUGAUCGGG 18 13320 HSV2-UL30-4967 + GCACUCGAGCUUGAUCGGG 19 13321

HSV2-UL30-1207 + CGCACUCGAGCUUGAUCGGG 20 4077

HSV2-UL30-4968 + UCGCACUCGAGCUUGAUCGGG 21 13322

HSV2-UL30-4969 + UUCGCACUCGAGCU UGAUCGGG 22 13323

HSV2-UL30-4970 + UU UCGCACUCGAGCUUGAUCGGG 23 13324

HSV2-UL30-4971 + UU UUCGCACUCGAGCUUGAUCGGG 24 13325

HSV2-UL30-4972 + CGCCUGCAGUCCCUCGGG 18 13326

HSV2-UL30-4973 + ACGCCUGCAGUCCCUCGGG 19 13327

HSV2-UL30-2405 + AACGCCUGCAGUCCCUCGGG 20 5167

HSV2-UL30-4974 + GAACGCCUGCAGUCCCUCGGG 21 13328

HSV2-UL30-4975 + CGAACGCCUGCAGUCCCUCGGG 22 13329

HSV2-UL30-4976 + CCGAACGCCUGCAGUCCCUCGGG 23 13330

HSV2-UL30-4977 + CCCGAACGCCUGCAGUCCCUCGGG 24 13331

HSV2-UL30-4978 + CUCGAGCU UGAUCGGGGG 18 13332

HSV2-UL30-4979 + ACUCGAGCUUGAUCGGGGG 19 13333

HSV2-UL30-2423 + CACUCGAGCUUGAUCGGGGG 20 5177

HSV2-UL30-4980 + GCACUCGAGCUUGAUCGGGGG 21 13334

HSV2-UL30-4981 + CGCACUCGAGCU UGAUCGGGGG 22 13335

HSV2-UL30-4982 + UCGCACUCGAGCUUGAUCGGGGG 23 13336

HSV2-UL30-4983 + UUCGCACUCGAGCUUGAUCGGGGG 24 13337

HSV2-UL30-4984 + UCGAGCU UGAUCGGGGGG 18 13338

HSV2-UL30-4985 + CUCGAGCUUGAUCGGGGGG 19 13339

HSV2-UL30-1208 + ACUCGAGCUUGAUCGGGGGG 20 4078

HSV2-UL30-4986 + CACUCGAGCU UGAUCGGGGGG 21 13340

HSV2-UL30-4987 + GCACUCGAGCUUGAUCGGGGGG 22 13341

HSV2-UL30-4988 + CGCACUCGAGCUUGAUCGGGGGG 23 13342

HSV2-UL30-4989 + UCGCACUCGAGCUUGAUCGGGGGG 24 13343

HSV2-UL30-4990 + GGCAGGGCCGCUGGGGGG 18 13344

HSV2-UL30-4991 + GGGCAGGGCCGCUGGGGGG 19 13345

HSV2-UL30-2379 + AGGGCAGGGCCGCUGGGGGG 20 5145

HSV2-UL30-4992 + GAGGGCAGGGCCGCUGGGGGG 21 13346

HSV2-UL30-4993 + GG AGGGCAGGGCCGCUGGGGGG 22 13347

HSV2-UL30-4994 + GGG AGGGCAGGGCCGCUGGGGGG 23 13348

HSV2-UL30-4995 + GGGGAGGGCAGGGCCGCUGGGGGG 24

13349

HSV2-UL30-4996 + UGAAACCCGGAGGUGGGG 18 13350

HSV2-UL30-4997 + GUGAAACCCGGAGGUGGGG 19 13351

HSV2-UL30-2480 + CG UGAAACCCGGAGGUGGGG 20 5215

HSV2-UL30-4998 + ACG UGAAACCCGGAGGUGGGG 21 13352

HSV2-UL30-4999 + GACG UGAAACCCGGAGGUGGGG 22 13353

HSV2-UL30-5000 + CG ACG UGAAACCCGGAGGUGGGG 23 13354

HSV2-UL30-5001 + UCGACGUGAAACCCGGAGGUGGGG 24 13355

HSV2-UL30-5002 + GGAUCCGGUCCUUGAUGG 18 13356

HSV2-UL30-5003 + GGGAUCCGGUCCU UGAUGG 19 13357

HSV2-UL30-2390 + CGGGAUCCGGUCCU UGAUGG 20 5153

HSV2-UL30-5004 + ACGGGAUCCGGUCCU UGAUGG 21 13358 HSV2-UL30-5005 + UACGGGAUCCGG UCCUUGAUGG 22 13359

HSV2-UL30-5006 + GUACGGGAUCCGGUCCU UGAUGG 23 13360

HSV2-UL30-5007 + CGUACGGGAUCCGGUCCUUGAUGG 24 13361

HSV2-UL30-5008 + GGAGGGCAGGGCCGCUGG 18 13362

HSV2-UL30-5009 + GGGAGGGCAGGGCCGCUGG 19 13363

HSV2-UL30-1137 + GGGGAGGGCAGGGCCGCUGG 20 4007

HSV2-UL30-5010 + CGGGGAGGGCAGGGCCGCUGG 21 13364

HSV2-UL30-5011 + CCGGGGAGGGCAGGGCCGCUGG 22 13365

HSV2-UL30-5012 + GCCGGGGAGGGCAGGGCCGCUGG 23 13366

HSV2-UL30-5013 + GGCCGGGGAGGGCAGGGCCGCUGG 24 13367

HSV2-UL30-5014 + UCUCCCGGGGGCGCUUGG 18 13368

HSV2-UL30-5015 + GUCUCCCGGGGGCGCUUGG 19 13369

HSV2-UL30-2368 + CGUCUCCCGGGGGCGCUUGG 20 5141

HSV2-UL30-5016 + GCGUCUCCCGGGGGCGCUUGG 21 13370

HSV2-UL30-5017 + GGCGUCUCCCGGGGGCGCUUGG 22 13371

HSV2-UL30-5018 + CGGCGUCUCCCGGGGGCGCU UGG 23 13372

HSV2-UL30-5019 + ACGGCGUCUCCCGGGGGCGCUUGG 24 13373

HSV2-UL30-5020 + AGACAGGAGAUCUGGAUG 18 13374

HSV2-UL30-5021 + CAGACAGGAGAUCUGGAUG 19 13375

HSV2-UL30-2554 + GCAGACAGGAGAUCUGGAUG 20 5271

HSV2-UL30-5022 + AGCAGACAGGAGAUCUGGAUG 21 13376

HSV2-UL30-5023 + GAGCAGACAGGAGAUCUGGAUG 22 13377

HSV2-UL30-5024 + AGAGCAGACAGGAGAUCUGGAUG 23 13378

HSV2-UL30-5025 + U AG AGCAGACAGGAGAUCUGGAUG 24 13379

HSV2-UL30-5026 + U UGUGGGCCUGGAUGAUG 18 13380

HSV2-UL30-5027 + GUUGUGGGCCUGGAUGAUG 19 13381

HSV2-UL30-2471 + GG UUGUGGGCCUGGAUGAUG 20 5210

HSV2-UL30-5028 + AGGUUGUGGGCCUGGAUGAUG 21 13382

HSV2-UL30-5029 + CAGG UUGUGGGCCUGGAUGAUG 22 13383

HSV2-UL30-5030 + ACAGG UUGUGGGCCUGGAUGAUG 23 13384

HSV2-UL30-5031 + CACAGGUUGUGGGCCUGGAUGAUG 24

13385

HSV2-UL30-5032 + GGGAGGGCAGGGCCGCUG 18 13386

HSV2-UL30-5033 + GGGGAGGGCAGGGCCGCUG 19 13387

HSV2-UL30-1136 + CGGGGAGGGCAGGGCCGCUG 20 4006

HSV2-UL30-5034 + CCGGGGAGGGCAGGGCCGCUG 21 13388

HSV2-UL30-5035 + GCCGGGGAGGGCAGGGCCGCUG 22 13389

HSV2-UL30-5036 + GGCCGGGGAGGGCAGGGCCGCUG 23 13390

HSV2-UL30-5037 + UGGCCGGGGAGGGCAGGGCCGCUG 24 13391

HSV2-UL30-5038 + GCGCCAGCCACUCCUCUG 18 13392

HSV2-UL30-5039 + CGCGCCAGCCACUCCUCUG 19 13393

HSV2-UL30-1187 + UCGCGCCAGCCACUCCUCUG 20 4057

HSV2-UL30-5040 + GUCGCGCCAGCCACUCCUCUG 21 13394

HSV2-UL30-5041 + GGUCGCGCCAGCCACUCCUCUG 22 13395

HSV2-UL30-5042 + GGGUCGCGCCAGCCACUCCUCUG 23 13396

HSV2-UL30-5043 + GGGGUCGCGCCAGCCACUCCUCUG 24 13397 HSV2-UL30-5044 + GAGAAGUAAUAGUCCGUG 18 13398

HSV2-UL30-5045 + CGAGAAGUAAUAGUCCGUG 19 13399

HSV2-UL30-2351 + GCGAGAAGUAAUAGUCCGUG 20 5132

HSV2-UL30-5046 + UGCGAGAAGUAAUAGUCCGUG 21 13400

HSV2-UL30-5047 + GUGCGAGAAGUAAUAGUCCGUG 22 13401

HSV2-UL30-5048 + GG UGCGAGAAGUAAUAGUCCGUG 23 13402

HSV2-UL30-5049 + AGGUGCGAGAAGUAAUAGUCCGUG 24 13403

HSV2-UL30-5050 + AUGUGCUCGAGGGCGGUG 18 13404

HSV2-UL30-5051 + GAUGUGCUCGAGGGCGGUG 19 13405

HSV2-UL30-2549 + GGAUGUGCUCGAGGGCGGUG 20 5267

HSV2-UL30-5052 + AGG AUGUGCUCGAGGGCGGUG 21 13406

HSV2-UL30-5053 + GAGG AUGUGCUCGAGGGCGGUG 22 13407

HSV2-UL30-5054 + GGAGGAUGUGCUCGAGGGCGGUG 23 13408

HSV2-UL30-5055 + AGGAGGAUGUGCUCGAGGGCGGUG 24

13409

HSV2-UL30-5056 + AGGUGCGGCAGAAACUUG 18 13410

HSV2-UL30-5057 + CAGGUGCGGCAGAAACUUG 19 13411

HSV2-UL30-2500 + CCAGGUGCGGCAGAAACU UG 20 5233

HSV2-UL30-5058 + UCCAGGUGCGGCAGAAACU UG 21 13412

HSV2-UL30-5059 + CUCCAGGUGCGGCAGAAACU UG 22 13413

HSV2-UL30-5060 + GCUCCAGGUGCGGCAGAAACUUG 23 13414

HSV2-UL30-5061 + AGCUCCAGGUGCGGCAGAAACUUG 24 13415

HSV2-UL30-5062 + ACCAGAUCCACGCCCUUG 18 13416

HSV2-UL30-5063 + CACCAGAUCCACGCCCUUG 19 13417

HSV2-UL30-2413 + GCACCAGAUCCACGCCCUUG 20 5172

HSV2-UL30-5064 + CGCACCAGAUCCACGCCCUUG 21 13418

HSV2-UL30-5065 + GCGCACCAGAUCCACGCCCUUG 22 13419

HSV2-UL30-5066 + UGCGCACCAGAUCCACGCCCUUG 23 13420

HSV2-UL30-5067 + UUGCGCACCAGAUCCACGCCCUUG 24 13421

HSV2-UL30-5068 + CGCUUGGGCGCCUCCUUG 18 13422

HSV2-UL30-5069 + GCGCUUGGGCGCCUCCUUG 19 13423

HSV2-UL30-2486 + GGCGCUUGGGCGCCUCCUUG 20 5221

HSV2-UL30-5070 + GGGCGCUUGGGCGCCUCCU UG 21 13424

HSV2-UL30-5071 + CGGGCGCU UGGGCGCCUCCUUG 22 13425

HSV2-UL30-5072 + CCGGGCGCUUGGGCGCCUCCUUG 23 13426

HSV2-UL30-5073 + GCCGGGCGCUUGGGCGCCUCCUUG 24 13427

HSV2-UL30-5074 + UACGGGAUCCGGUCCU UG 18 13428

HSV2-UL30-5075 + GUACGGGAUCCGGUCCUUG 19 13429

HSV2-UL30-2389 + CGUACGGGAUCCGGUCCUUG 20 5152

HSV2-UL30-5076 + ACGUACGGGAUCCGGUCCU UG 21 13430

HSV2-UL30-5077 + CACGUACGGGAUCCGGUCCUUG 22 13431

HSV2-UL30-5078 + UCACGUACGGGAUCCGGUCCUUG 23 13432

HSV2-UL30-5079 + AUCACGUACGGGAUCCGGUCCUUG 24 13433

HSV2-UL30-5080 + UCGGACACCAGCAGCUUG 18 13434

HSV2-UL30-5081 + CUCGGACACCAGCAGCUUG 19 13435

HSV2-UL30-1111 + GCUCGGACACCAGCAGCUUG 20 3981 HSV2-UL30-5082 + AGCUCGGACACCAGCAGCUUG 21 13436

HSV2-UL30-5083 + CAGCUCGG ACACCAGCAGCU UG 22 13437

HSV2-UL30-5084 + CCAGCUCGGACACCAGCAGCUUG 23 13438

HSV2-UL30-5085 + GCCAGCUCGGACACCAGCAGCUUG 24 13439

HSV2-UL30-5086 + GCGAUGAGCAGCAGCUUG 18 13440

HSV2-UL30-5087 + GGCGAUGAGCAGCAGCUUG 19 13441

HSV2-UL30-2415 + UGGCGAUGAGCAGCAGCUUG 20 5174

HSV2-UL30-5088 + UUGGCGAUGAGCAGCAGCUUG 21 13442

HSV2-UL30-5089 + CU UGGCGAUGAGCAGCAGCU UG 22 13443

HSV2-UL30-5090 + UCU UGGCGAUGAGCAGCAGCU UG 23 13444

HSV2-UL30-5091 + UUCUUGGCGAUGAGCAGCAGCUUG 24 13445

HSV2-UL30-5092 + UCGGGGCCGUACUGCU UG 18 13446

HSV2-UL30-5093 + CUCGGGGCCGUACUGCU UG 19 13447

HSV2-UL30-2524 + ACUCGGGGCCGUACUGCUUG 20 5250

HSV2-UL30-5094 + AACUCGGGGCCGUACUGCU UG 21 13448

HSV2-UL30-5095 + GAACUCGGGGCCGUACUGCUUG 22 13449

HSV2-UL30-5096 + CGAACUCGGGGCCGUACUGCUUG 23 13450

HSV2-UL30-5097 + ACGAACUCGGGGCCGUACUGCU UG 24 13451

HSV2-UL30-5098 + AUGGCGGCCUGUUGCU UG 18 13452

HSV2-UL30-5099 + GAUGGCGGCCUGUUGCUUG 19 13453

HSV2-UL30-2446 + UGAUGGCGGCCUGUUGCU UG 20 5193

HSV2-UL30-5100 + UUGAUGGCGGCCUGUUGCU UG 21 13454

HSV2-UL30-5101 + CUUGAUGGCGGCCUGUUGCUUG 22 13455

HSV2-UL30-5102 + CCUUGAUGGCGGCCUG UUGCU UG 23 13456

HSV2-UL30-5103 + ACCUUGAUGGCGGCCUGUUGCUUG 24 13457

HSV2-UL30-5104 + UCGGGGAGGUCGCAGGAU 18 13458

HSV2-UL30-5105 + CUCGGGGAGGUCGCAGGAU 19 13459

HSV2-UL30-2542 + ACUCGGGGAGGUCGCAGGAU 20 5261

HSV2-UL30-5106 + GACUCGGGGAGGUCGCAGGAU 21 13460

HSV2-UL30-5107 + GGACUCGGGGAGGUCGCAGGAU 22 13461

HSV2-UL30-5108 + GGG ACUCGGGGAGGUCGCAGGAU 23 13462

HSV2-UL30-5109 + UGGGACUCGGGGAGGUCGCAGGAU 24

13463

HSV2-UL30-5110 + UUCGCACUCGAGCUUGAU 18 13464

HSV2-UL30-5111 + UUUCGCACUCGAGCUUGAU 19 13465

HSV2-UL30-1203 + UUU UCGCACUCGAGCUUGAU 20 4073

HSV2-UL30-5112 + UUU UUCGCACUCGAGCU UGAU 21 13466

HSV2-UL30-5113 + UUU UUUCGCACUCGAGCUUGAU 22 13467

HSV2-UL30-5114 + GUUU UUUCGCACUCGAGCU UGAU 23 13468

HSV2-UL30-5115 + CGU UUUUUCGCACUCGAGCUUGAU 24 13469

HSV2-UL30-5116 + U G U ACCCG G U C ACG A AC U 18 13470

HSV2-UL30-5117 + UUGUACCCGGUCACGAACU 19 13471

HSV2-UL30-1337 + GUUGUACCCGGUCACGAACU 20 4207

HSV2-UL30-5118 + UGUUGUACCCGGUCACGAACU 21 13472

HSV2-UL30-5119 + AUGUUGUACCCGGUCACGAACU 22 13473

HSV2-UL30-5120 + GAUGU UGUACCCGGUCACGAACU 23 13474 HSV2-UL30-5121 + UGAUGUUGUACCCGGUCACGAACU 24 13475

HSV2-UL30-5122 + GAUCGCUGAGGUGGGACU 18 13476

HSV2-UL30-5123 + AGAUCGCUGAGGUGGGACU 19 13477

HSV2-UL30-1354 + GAGAUCGCUGAGGUGGGACU 20 4224

HSV2-UL30-5124 + CGAGAUCGCUGAGGUGGGACU 21 13478

HSV2-UL30-5125 + GCG AGAUCGCUGAGGUGGGACU 22 13479

HSV2-UL30-5126 + GGCG AGAUCGCUGAGGUGGGACU 23 13480

HSV2-UL30-5127 + AGGCGAGAUCGCUGAGGUGGGACU 24 13481

HSV2-UL30-5128 + CGCGCAUGCCGGCCGCCU 18 13482

HSV2-UL30-5129 + GCGCGCAUGCCGGCCGCCU 19 13483

HSV2-UL30-2436 + GGCGCGCAUGCCGGCCGCCU 20 5185

HSV2-UL30-5130 + GGGCGCGCAUGCCGGCCGCCU 21 13484

HSV2-UL30-5131 + GGGGCGCGCAUGCCGGCCGCCU 22 13485

HSV2-UL30-5132 + GGGGGCGCGCAUGCCGGCCGCCU 23 13486

HSV2-UL30-5133 + CGGGGGCGCGCAUGCCGGCCGCCU 24 13487

HSV2-UL30-5134 + ACGGCGACGCGAUGGCCU 18 13488

HSV2-UL30-5135 + AACGGCGACGCGAUGGCCU 19 13489

HSV2-UL30-2588 + GAACGGCGACGCGAUGGCCU 20 5297

HSV2-UL30-5136 + UG AACGGCGACGCGAUGGCCU 21 13490

HSV2-UL30-5137 + GUGAACGGCGACGCGAUGGCCU 22 13491

HSV2-UL30-5138 + CGUG AACGGCGACGCGAUGGCCU 23 13492

HSV2-UL30-5139 + ACGUG AACGGCGACGCGAUGGCCU 24 13493

HSV2-UL30-5140 + CGAGGAGGACGGCCUCCU 18 13494

HSV2-UL30-5141 + UCGAGGAGGACGGCCUCCU 19 13495

HSV2-UL30-1240 + GUCGAGGAGGACGGCCUCCU 20 4110

HSV2-UL30-5142 + UGUCGAGGAGGACGGCCUCCU 21 13496

HSV2-UL30-5143 + UUGUCGAGGAGGACGGCCUCCU 22 13497

HSV2-UL30-5144 + CUUGUCGAGGAGGACGGCCUCCU 23 13498

HSV2-UL30-5145 + GCUUGUCGAGGAGGACGGCCUCCU 24 13499

HSV2-UL30-5146 + GGGGAGGGCAGGGCCGCU 18 13500

HSV2-UL30-5147 + CGGGGAGGGCAGGGCCGCU 19 13501

HSV2-UL30-1135 + CCGGGGAGGGCAGGGCCGCU 20 4005

HSV2-UL30-5148 + GCCGGGGAGGGCAGGGCCGCU 21 13502

HSV2-UL30-5149 + GGCCGGGGAGGGCAGGGCCGCU 22 13503

HSV2-UL30-5150 + UGGCCGGGGAGGGCAGGGCCGCU 23 13504

HSV2-UL30-5151 + UUGGCCGGGGAGGGCAGGGCCGCU 24 13505

HSV2-UL30-5152 + GGCCUCCUCGGGGGUGCU 18 13506

HSV2-UL30-5153 + CGGCCUCCUCGGGGGUGCU 19 13507

HSV2-UL30-2453 + ACGGCCUCCUCGGGGGUGCU 20 5197

HSV2-UL30-5154 + GACGGCCUCCUCGGGGGUGCU 21 13508

HSV2-UL30-5155 + GGACGGCCUCCUCGGGGGUGCU 22 13509

HSV2-UL30-5156 + AGG ACGGCCUCCUCGGGGGUGCU 23 13510

HSV2-UL30-5157 + GAGGACGGCCUCCUCGGGGGUGCU 24 13511

HSV2-UL30-5158 + CGCGCCAGCCACUCCUCU 18 13512

HSV2-UL30-5159 + UCGCGCCAGCCACUCCUCU 19 13513

HSV2-UL30-2407 + GUCGCGCCAGCCACUCCUCU 20 5168 HSV2-UL30-5160 + GGUCGCGCCAGCCACUCCUCU 21 13514

HSV2-UL30-5161 + GGGUCGCGCCAGCCACUCCUCU 22 13515

HSV2-UL30-5162 + GGGGUCGCGCCAGCCACUCCUCU 23 13516

HSV2-UL30-5163 + AG GG G U CG CG CCAG CCACU CCU CU 24 13517

HSV2-UL30-5164 + CCUCCUCGGGGGUGCUCU 18 13518

HSV2-UL30-5165 + GCCUCCUCGGGGGUGCUCU 19 13519

HSV2-UL30-1245 + GGCCUCCUCGGGGGUGCUCU 20 4115

HSV2-UL30-5166 + CGGCCUCCUCGGGGGUGCUCU 21 13520

HSV2-UL30-5167 + ACGGCCUCCUCGGGGGUGCUCU 22 13521

HSV2-UL30-5168 + GACGGCCUCCUCGGGGGUGCUCU 23 13522

HSV2-UL30-5169 + GGACGGCCUCCUCGGGGGUGCUCU 24 13523

HSV2-UL30-5170 + CCGGGGGGUGCCACGUCU 18 13524

HSV2-UL30-5171 + UCCGGGGGGUGCCACGUCU 19 13525

HSV2-UL30-1096 + GUCCGGGGGGUGCCACGUCU 20 3966

HSV2-UL30-5172 + CGUCCGGGGGGUGCCACGUCU 21 13526

HSV2-UL30-5173 + UCGUCCGGGGGGUGCCACGUCU 22 13527

HSV2-UL30-5174 + GUCGUCCGGGGGGUGCCACGUCU 23 13528

HSV2-UL30-5175 + CGUCGUCCGGGGGGUGCCACGUCU 24 13529

HSV2-UL30-5176 + AUGCGCCGAUGGGCGUCU 18 13530

HSV2-UL30-5177 + GAUGCGCCGAUGGGCGUCU 19 13531

HSV2-UL30-2401 + UGAUGCGCCGAUGGGCGUCU 20 5163

HSV2-UL30-5178 + GUG AUGCGCCGAUGGGCGUCU 21 13532

HSV2-UL30-5179 + GG UGAUGCGCCGAUGGGCGUCU 22 13533

HSV2-UL30-5180 + CGG UGAUGCGCCGAUGGGCGUCU 23 13534

HSV2-UL30-5181 + UCGGUGAUGCGCCGAUGGGCGUCU 24 13535

HSV2-UL30-5182 + AGAUGAUGCGCAUGGAGU 18 13536

HSV2-UL30-5183 + UAGAUGAUGCGCAUGGAGU 19 13537

HSV2-UL30-2432 + GU AGAUGAUGCGCAUGGAGU 20 5183

HSV2-UL30-5184 + CGU AGAUGAUGCGCAUGGAGU 21 13538

HSV2-UL30-5185 + CCG UAGAUGAUGCGCAUGGAGU 22 13539

HSV2-UL30-5186 + CCCGU AGAUGAUGCGCAUGGAGU 23 13540

HSV2-UL30-5187 + CCCCGUAGAUGAUGCGCAUGGAGU 24 13541

HSV2-UL30-5188 + UCUGGGCCACGAUCACGU 18 13542

HSV2-UL30-5189 + GUCUGGGCCACGAUCACGU 19 13543

HSV2-UL30-2387 + GGUCUGGGCCACGAUCACGU 20 5151

HSV2-UL30-5190 + GGG UCUGGGCCACGAUCACGU 21 13544

HSV2-UL30-5191 + CGGGUCUGGGCCACGAUCACGU 22 13545

HSV2-UL30-5192 + GCGGGUCUGGGCCACGAUCACGU 23 13546

HSV2-UL30-5193 + CGCGGGUCUGGGCCACGAUCACGU 24 13547

HSV2-UL30-5194 + CG AGG U UCCG AACGCCG U 18 13548

HSV2-UL30-5195 + UCGAGGU UCCGAACGCCGU 19 13549

HSV2-UL30-1382 + CUCGAGGUUCCGAACGCCGU 20 4252

HSV2-UL30-5196 + GCUCGAGGUUCCGAACGCCGU 21 13550

HSV2-UL30-5197 + CGCUCGAGGUUCCGAACGCCGU 22 13551

HSV2-UL30-5198 + UCGCUCGAGGUUCCGAACGCCGU 23 13552

HSV2-UL30-5199 + GUCGCUCGAGGU UCCGAACGCCGU 24 13553 HSV2-UL30-5200 + GCCGCGCGGCCACGUCGU 18 13554

HSV2-UL30-5201 + AGCCGCGCGGCCACGUCGU 19 13555

HSV2-UL30-2340 + GAGCCGCGCGGCCACGUCGU 20 5126

HSV2-UL30-5202 + UGAGCCGCGCGGCCACGUCGU 21 13556

HSV2-UL30-5203 + CUGAGCCGCGCGGCCACGUCGU 22 13557

HSV2-UL30-5204 + CCUGAGCCGCGCGGCCACGUCGU 23 13558

HSV2-UL30-5205 + CCCUGAGCCGCGCGGCCACGUCGU 24 13559

HSV2-UL30-5206 + AGGCCGCCCCCAGCAGGU 18 13560

HSV2-UL30-5207 + CAGGCCGCCCCCAGCAGGU 19 13561

HSV2-UL30-2349 + GCAGGCCGCCCCCAGCAGGU 20 5130

HSV2-UL30-5208 + CGCAGGCCGCCCCCAGCAGGU 21 13562

HSV2-UL30-5209 + ACGCAGGCCGCCCCCAGCAGGU 22 13563

HSV2-UL30-5210 + CACGCAGGCCGCCCCCAGCAGGU 23 13564

HSV2-UL30-5211 + UCACGCAGGCCGCCCCCAGCAGGU 24 13565

HSV2-UL30-5212 + UCUGCUCAGU UCGGCGGU 18 13566

HSV2-UL30-5213 + GUCUGCUCAGU UCGGCGGU 19 13567

HSV2-UL30-2397 + UGUCUGCUCAGUUCGGCGGU 20 5159

HSV2-UL30-5214 + GUGUCUGCUCAGUUCGGCGGU 21 13568

HSV2-UL30-5215 + GGUGUCUGCUCAGUUCGGCGGU 22 13569

HSV2-UL30-5216 + GGGUGUCUGCUCAGUUCGGCGGU 23 13570

HSV2-UL30-5217 + CGGGUGUCUGCUCAGU UCGGCGGU 24 13571

HSV2-UL30-5218 + CUCCUCUACCUCGCGGGU 18 13572

HSV2-UL30-5219 + UCUCCUCUACCUCGCGGGU 19 13573

HSV2-UL30-2386 + GUCUCCUCUACCUCGCGGGU 20 5150

HSV2-UL30-5220 + CGUCUCCUCUACCUCGCGGGU 21 13574

HSV2-UL30-5221 + CCGUCUCCUCUACCUCGCGGGU 22 13575

HSV2-UL30-5222 + ACCGUCUCCUCUACCUCGCGGGU 23 13576

HSV2-UL30-5223 + GACCGUCUCCUCUACCUCGCGGGU 24 13577

HSV2-UL30-5224 + GAUCUCCGUCAGCU UGGU 18 13578

HSV2-UL30-5225 + AGAUCUCCGUCAGCUUGGU 19 13579

HSV2-UL30-2517 + UAGAUCUCCGUCAGCU UGGU 20 5244

HSV2-UL30-5226 + GUAGAUCUCCGUCAGCU UGGU 21 13580

HSV2-UL30-5227 + UGUAGAUCUCCGUCAGCUUGGU 22 13581

HSV2-UL30-5228 + UUGUAGAUCUCCGUCAGCUUGGU 23 13582

HSV2-UL30-5229 + CUUGUAGAUCUCCGUCAGCUUGGU 24 13583

HSV2-UL30-5230 + CGGCGACGCGAUGGCCU U 18 13584

HSV2-UL30-5231 + ACGGCG ACGCG AUGGCCU U 19 13585

HSV2-UL30-1429 + AACGGCGACGCGAUGGCCUU 20 4299

HSV2-UL30-5232 + G AACGGCG ACGCG AUGGCCU U 21 13586

HSV2-UL30-5233 + UG AACGGCGACGCGAUGGCCUU 22 13587

HSV2-UL30-5234 + GUGAACGGCG ACGCG AUGGCCU U 23 13588

HSV2-UL30-5235 + CGUGAACGGCGACGCGAUGGCCUU 24 13589

HSV2-UL30-5236 + CUCGG ACACCAGCAGCU U 18 13590

HSV2-UL30-5237 + GCUCGGACACCAGCAGCUU 19 13591

HSV2-UL30-2358 + AGCUCGGACACCAGCAGCUU 20 5137

HSV2-UL30-5238 + CAGCUCGGACACCAGCAGCU U 21 13592 HSV2-UL30-5239 + CCAGCUCGG ACACCAGCAGCU U 22 13593

HSV2-UL30-5240 + GCCAGCUCGGACACCAGCAGCU U 23 13594

HSV2-UL30-5241 + CGCCAGCUCGGACACCAGCAGCUU 24 13595

HSV2-UL30-5242 + CUUGAUCUUGCUGCGCU U 18 13596

HSV2-UL30-5243 + CCUUGAUCUUGCUGCGCUU 19 13597

HSV2-UL30-2511 + ACCU UGAUCUUGCUGCGCU U 20 5240

HSV2-UL30-5244 + CACCUUGAUCUUGCUGCGCUU 21 13598

HSV2-UL30-5245 + UCACCUUGAUCUUGCUGCGCUU 22 13599

HSV2-UL30-5246 + UUCACCUUGAUCUUGCUGCGCUU 23 13600

HSV2-UL30-5247 + GUUCACCU UGAUCUUGCUGCGCUU 24 13601

HSV2-UL30-5248 + UCUGGAUGACGAGGUCU U 18 13602

HSV2-UL30-5249 + AUCUGGAUGACGAGGUCUU 19 13603

HSV2-UL30-2555 + GAUCUGGAUGACGAGGUCUU 20 5272

HSV2-UL30-5250 + AGAUCUGGAUGACGAGGUCUU 21 13604

HSV2-UL30-5251 + GAGAUCUGGAUGACGAGGUCUU 22 13605

HSV2-UL30-5252 + GGAGAUCUGGAUGACGAGGUCU U 23 13606

HSV2-UL30-5253 + AGGAGAUCUGGAUGACGAGGUCUU 24

13607

HSV2-UL30-5254 + CGUACUGAAGCACAGGUU 18 13608

HSV2-UL30-5255 + GCG UACUGAAGCACAGGUU 19 13609

HSV2-UL30-2469 + AGCGUACUGAAGCACAGGU U 20 5208

HSV2-UL30-5256 + GAGCGUACUGAAGCACAGGUU 21 13610

HSV2-UL30-5257 + AG AGCGUACUGAAGCACAGGUU 22 13611

HSV2-UL30-5258 + GAGAGCGUACUGAAGCACAGGUU 23 13612

HSV2-UL30-5259 + GGAGAGCGUACUGAAGCACAGGU U 24 13613

HSV2-UL30-5260 + UCGACGUCGCUCGAGGU U 18 13614

HSV2-UL30-5261 + CUCGACGUCGCUCGAGGUU 19 13615

HSV2-UL30-2561 + ACUCGACGUCGCUCGAGGUU 20 5277

HSV2-UL30-5262 + AACUCGACGUCGCUCGAGGUU 21 13616

HSV2-UL30-5263 + AAACUCGACGUCGCUCGAGGUU 22 13617

HSV2-UL30-5264 + UAAACUCGACGUCGCUCGAGGU U 23 13618

HSV2-UL30-5265 + UUAAACUCGACGUCGCUCGAGGUU 24 13619

HSV2-UL30-5266 - UUUGUCCUCACCGCCGAA 18 13620

HSV2-UL30-5267 - CU UUGUCCUCACCGCCGAA 19 13621

HSV2-UL30-2015 - ACU UUGUCCUCACCGCCGAA 20 4914

HSV2-UL30-5268 - GACUUUGUCCUCACCGCCGAA 21 13622

HSV2-UL30-5269 - GGACUUUGUCCUCACCGCCGAA 22 13623

HSV2-UL30-5270 - AGGACUU UGUCCUCACCGCCGAA 23 13624

HSV2-UL30-5271 - CAGGACUUUGUCCUCACCGCCGAA 24 13625

HSV2-UL30-5272 - ACCGCGUCUUCGUGCGAA 18 13626

HSV2-UL30-5273 - UACCGCGUCUUCGUGCGAA 19 13627

HSV2-UL30-1812 - CUACCGCGUCUUCGUGCGAA 20 4784

HSV2-UL30-5274 - ACUACCGCG UCUUCGUGCGAA 21 13628

HSV2-UL30-5275 - UACUACCGCGUCU UCGUGCGAA 22 13629

HSV2-UL30-5276 - GUACUACCGCGUCUUCGUGCGAA 23 13630

HSV2-UL30-5277 - UGUACUACCGCGUCU UCGUGCGAA 24 13631 HSV2-UL30-5278 - GCGGCCGGGGGACGGGAA 18 13632

HSV2-UL30-5279 - AGCGGCCGGGGGACGGGAA 19 13633

HSV2-UL30-589 - GAGCGGCCGGGGGACGGGAA 20 3459

HSV2-UL30-5280 - GGAGCGGCCGGGGGACGGGAA 21 13634

HSV2-UL30-5281 - GGG AGCGGCCGGGGGACGGGAA 22 13635

HSV2-UL30-5282 - GGGG AGCGGCCGGGGGACGGGAA 23 13636

HSV2-UL30-5283 - AGGGGAGCGGCCGGGGGACGGGAA 24 13637

HSV2-UL30-5284 - GCCGUGCCUCGGGGGGAA 18 13638

HSV2-UL30-5285 - GGCCGUGCCUCGGGGGGAA 19 13639

HSV2-UL30-580 - CGGCCGUGCCUCGGGGGGAA 20 3450

HSV2-UL30-5286 - CCGGCCGUGCCUCGGGGGGAA 21 13640

HSV2-UL30-5287 - CCCGGCCGUGCCUCGGGGGGAA 22 13641

HSV2-UL30-5288 - GCCCGGCCGUGCCUCGGGGGGAA 23 13642

HSV2-UL30-5289 - CGCCCGGCCGUGCCUCGGGGGGAA 24 13643

HSV2-UL30-5290 - GGUUUCGGGGCCUCGACA 18 13644

HSV2-UL30-5291 - CGGU UUCGGGGCCUCGACA 19 13645

HSV2-UL30-571 - GCGGUUUCGGGGCCUCGACA 20 3441

HSV2-UL30-5292 - GGCGGUUUCGGGGCCUCGACA 21 13646

HSV2-UL30-5293 - GGGCGGUUUCGGGGCCUCGACA 22 13647

HSV2-UL30-5294 - GGGGCGGUU UCGGGGCCUCGACA 23 13648

HSV2-UL30-5295 - AGGGGCGGUU UCGGGGCCUCGACA 24 13649

HSV2-UL30-5296 - CCGGCACGUUGGGUACCA 18 13650

HSV2-UL30-5297 - GCCGGCACGUUGGGUACCA 19 13651

HSV2-UL30-612 - GGCCGGCACGU UGGGUACCA 20 3482

HSV2-UL30-5298 - GGGCCGGCACG UUGGGUACCA 21 13652

HSV2-UL30-5299 - GGGGCCGGCACGUUGGGUACCA 22 13653

HSV2-UL30-5300 - GGGGGCCGGCACGUUGGGUACCA 23 13654

HSV2-UL30-5301 - CGGGGGCCGGCACGU UGGGUACCA 24 13655

HSV2-UL30-5302 - CUAGACGCCGCCGCCCCA 18 13656

HSV2-UL30-5303 - GCUAGACGCCGCCGCCCCA 19 13657

HSV2-UL30-718 - AGCUAGACGCCGCCGCCCCA 20 3588

HSV2-UL30-5304 - GAGCUAGACGCCGCCGCCCCA 21 13658

HSV2-UL30-5305 - CGAGCUAGACGCCGCCGCCCCA 22 13659

HSV2-UL30-5306 - GCGAGCUAGACGCCGCCGCCCCA 23 13660

HSV2-UL30-5307 - CGCGAGCUAGACGCCGCCGCCCCA 24 13661

HSV2-UL30-5308 - U UAGCCGAGCGCCCCGCA 18 13662

HSV2-UL30-5309 - GUUAGCCGAGCGCCCCGCA 19 13663

HSV2-UL30-2001 - CGU UAGCCGAGCGCCCCGCA 20 4905

HSV2-UL30-5310 - G CG U U AG CCG AG CG CCCCG CA 21 13664

HSV2-UL30-5311 - CGCGU UAGCCGAGCGCCCCGCA 22 13665

HSV2-UL30-5312 - CCGCGUUAGCCGAGCGCCCCGCA 23 13666

HSV2-UL30-5313 - GCCGCGU UAGCCGAGCGCCCCGCA 24 13667

HSV2-UL30-5314 - AACUCGUCGAAUGU UGCA 18 13668

HSV2-UL30-5315 - AAACUCGUCGAAUGU UGCA 19 13669

HSV2-UL30-2063 - GAAACUCGUCGAAUGUUGCA 20 4945

HSV2-UL30-5316 - GGAAACUCGUCGAAUGU UGCA 21 13670 HSV2-UL30-5317 - AGGAAACUCGUCGAAUGUUGCA 22 13671

HSV2-UL30-5318 - GAGGAAACUCGUCGAAUGUUGCA 23 13672

HSV2-UL30-5319 - GGAGGAAACUCGUCGAAUGU UGCA 24 13673

HSV2-UL30-5320 - GAAAUAACGCCAAGAUCA 18 13674

HSV2-UL30-5321 - GGAAAUAACGCCAAGAUCA 19 13675

HSV2-UL30-2046 - UGGAAAUAACG CCA AG A U C A 20 4933

HSV2-UL30-5322 - UUGGAAAUAACGCCAAGAUCA 21 13676

HSV2-UL30-5323 - UUUGGAAAUAACGCCAAGAUCA 22 13677

HSV2-UL30-5324 - GU U UGGAAAUAACGCCAAGAUCA 23 13678

HSV2-UL30-5325 - UGUU UGGAAAUAACGCCAAGAUCA 24 13679

HSV2-UL30-5326 - ACUCGGUGUACGGGUUCA 18 13680

HSV2-UL30-5327 - AACUCGGUGUACGGGU UCA 19 13681

HSV2-UL30-1973 - CAACUCGGUGUACGGGU UCA 20 4885

HSV2-UL30-5328 - GCAACUCGGUGUACGGGUUCA 21 13682

HSV2-UL30-5329 - UGCAACUCGGUGUACGGGUUCA 22 13683

HSV2-UL30-5330 - GUGCAACUCGGUGUACGGGUUCA 23 13684

HSV2-UL30-5331 - UGUGCAACUCGGUGUACGGGUUCA 24 13685

HSV2-UL30-5332 - AGGAGGUCGCGCGCGAGA 18 13686

HSV2-UL30-5333 - GAGGAGGUCGCGCGCGAGA 19 13687

HSV2-UL30-1940 - CG AGGAGGUCGCGCGCGAGA 20 4863

HSV2-UL30-5334 - GCGAGGAGGUCGCGCGCGAGA 21 13688

HSV2-UL30-5335 - CGCG AGGAGGUCGCGCGCGAGA 22 13689

HSV2-UL30-5336 - GCGCG AGGAGGUCGCGCGCGAGA 23 13690

HSV2-UL30-5337 - AGCGCGAGGAGGUCGCGCGCGAGA 24 13691

HSV2-UL30-5338 - ACCGGGACUACCUGGAGA 18 13692

HSV2-UL30-5339 - GACCGGGACUACCUGGAGA 19 13693

HSV2-UL30-1957 - GG ACCGGGACUACCUGGAGA 20 4873

HSV2-UL30-5340 - CGG ACCGGGACUACCUGGAGA 21 13694

HSV2-UL30-5341 - GCGG ACCGGGACUACCUGGAGA 22 13695

HSV2-UL30-5342 - GGCGG ACCGGGACUACCUGGAGA 23 13696

HSV2-UL30-5343 - AGGCGGACCGGGACUACCUGGAGA 24 13697

HSV2-UL30-5344 - CGCGAUCAGGAAGUACGA 18 13698

HSV2-UL30-5345 - CCGCGAUCAGGAAGUACGA 19 13699

HSV2-UL30-474 - CCCGCGAUCAGGAAGUACGA 20 3344

HSV2-UL30-5346 - CCCCGCGAUCAGGAAGUACGA 21 13700

HSV2-UL30-5347 - GCCCCGCGAUCAGGAAGUACGA 22 13701

HSV2-UL30-5348 - UGCCCCGCGAUCAGGAAGUACGA 23 13702

HSV2-UL30-5349 - UUGCCCCGCGAUCAGGAAGUACGA 24 13703

HSV2-UL30-5350 - GGACAACCUGGCCGUCGA 18 13704

HSV2-UL30-5351 - CGGACAACCUGGCCGUCGA 19 13705

HSV2-UL30-495 - GCGGACAACCUGGCCGUCGA 20 3365

HSV2-UL30-5352 - GGCGGACAACCUGGCCGUCGA 21 13706

HSV2-UL30-5353 - CGGCGGACAACCUGGCCGUCGA 22 13707

HSV2-UL30-5354 - ACGGCGGACAACCUGGCCGUCGA 23 13708

HSV2-UL30-5355 - CACGGCGGACAACCUGGCCGUCGA 24 13709

HSV2-UL30-5356 - UAAGGACGACGACGAGGA 18 13710 HSV2-UL30-5357 - AUAAGGACGACGACGAGGA 19 13711

HSV2-UL30-595 - GAUAAGGACGACGACGAGGA 20 3465

HSV2-UL30-5358 - GG AUAAGGACGACGACGAGGA 21 13712

HSV2-UL30-5359 - AGG AUAAGGACGACGACGAGGA 22 13713

HSV2-UL30-5360 - GAGG AUAAGGACGACGACGAGGA 23 13714

HSV2-UL30-5361 - CGAGGAUAAGGACGACGACGAGGA 24 13715

HSV2-UL30-5362 - CGAGGACGGGGACGAGGA 18 13716

HSV2-UL30-5363 - ACGAGGACGGGGACGAGGA 19 13717

HSV2-UL30-599 - GACGAGGACGGGGACGAGGA 20 3469

HSV2-UL30-5364 - CG ACGAGGACGGGGACGAGGA 21 13718

HSV2-UL30-5365 - ACG ACGAGGACGGGGACGAGGA 22 13719

HSV2-UL30-5366 - GACG ACGAGGACGGGGACGAGGA 23 13720

HSV2-UL30-5367 - CGACGACGAGGACGGGGACGAGGA 24 13721

HSV2-UL30-5368 - GCGCAUCACCGACCCGGA 18 13722

HSV2-UL30-5369 - GGCGCAUCACCGACCCGGA 19 13723

HSV2-UL30-2011 - CG G CG CA U CACCG ACCCGG A 20 4911

HSV2-UL30-5370 - UCGGCGCAUCACCGACCCGGA 21 13724

HSV2-UL30-5371 - AUCGGCGCAUCACCGACCCGGA 22 13725

HSV2-UL30-5372 - CAUCGGCGCAUCACCGACCCGGA 23 13726

HSV2-UL30-5373 - CCAUCGGCGCAUCACCGACCCGGA 24 13727

HSV2-UL30-5374 - CGCGCACCUGGAGGCGGA 18 13728

HSV2-UL30-5375 - UCGCGCACCUGGAGGCGGA 19 13729

HSV2-UL30-1953 - GUCGCGCACCUGGAGGCGGA 20 4871

HSV2-UL30-5376 - CG UCGCGCACCUGGAGGCGGA 21 13730

HSV2-UL30-5377 - CCG UCGCGCACCUGGAGGCGGA 22 13731

HSV2-UL30-5378 - GCCG UCGCGCACCUGGAGGCGGA 23 13732

HSV2-UL30-5379 - GGCCG UCGCGCACCUGGAGGCGGA 24 13733

HSV2-UL30-5380 - AGCGGCCGGGGGACGGGA 18 13734

HSV2-UL30-5381 - GAGCGGCCGGGGGACGGGA 19 13735

HSV2-UL30-1919 - GG AGCGGCCGGGGGACGGGA 20 4849

HSV2-UL30-5382 - GGG AGCGGCCGGGGGACGGGA 21 13736

HSV2-UL30-5383 - GGGG AGCGGCCGGGGGACGGGA 22 13737

HSV2-UL30-5384 - AGGGG AGCGGCCGGGGGACGGGA 23 13738

HSV2-UL30-5385 - AAGGGGAGCGGCCGGGGGACGGGA 24 13739

HSV2-UL30-5386 - AGGGGAGCGGCCGGGGGA 18 13740

HSV2-UL30-5387 - AAGGGGAGCGGCCGGGGGA 19 13741

HSV2-UL30-587 - GAAGGGGAGCGGCCGGGGGA 20 3457

HSV2-UL30-5388 - GG AAGGGGAGCGGCCGGGGGA 21 13742

HSV2-UL30-5389 - GGG AAGGGGAGCGGCCGGGGGA 22 13743

HSV2-UL30-5390 - GGGG AAGGGGAGCGGCCGGGGGA 23 13744

HSV2-UL30-5391 - GGGGGAAGGGGAGCGGCCGGGGGA 24

13745

HSV2-UL30-5392 - GGCCGUGCCUCGGGGGGA 18 13746

HSV2-UL30-5393 - CGGCCGUGCCUCGGGGGGA 19 13747

HSV2-UL30-579 - CCGGCCGUGCCUCGGGGGGA 20 3449

HSV2-UL30-5394 - CCCGGCCGUGCCUCGGGGGGA 21 13748 HSV2-UL30-5395 - GCCCGGCCGUGCCUCGGGGGGA 22 13749

HSV2-UL30-5396 - CGCCCGGCCGUGCCUCGGGGGGA 23 13750

HSV2-UL30-5397 - GCGCCCGGCCGUGCCUCGGGGGGA 24 13751

HSV2-UL30-5398 - UCGUCCUGACCAAGCUGA 18 13752

HSV2-UL30-5399 - UUCGUCCUGACCAAGCUGA 19 13753

HSV2-UL30-522 - CU UCGUCCUGACCAAGCUGA 20 3392

HSV2-UL30-5400 - CCUUCGUCCUGACCAAGCUGA 21 13754

HSV2-UL30-5401 - CCCUUCGUCCUGACCAAGCUGA 22 13755

HSV2-UL30-5402 - GCCCUUCGUCCUGACCAAGCUGA 23 13756

HSV2-UL30-5403 - GGCCCUUCGUCCUGACCAAGCUGA 24 13757

HSV2-UL30-5404 - GCAGCAAGAUCAAGGUGA 18 13758

HSV2-UL30-5405 - CG C AG C A AG A U C A AG G U G A 19 13759

HSV2-UL30-1872 - GCGCAGCAAGAUCAAGGUGA 20 4821

HSV2-UL30-5406 - AGCGCAGCAAGAUCAAGGUGA 21 13760

HSV2-UL30-5407 - AAGCGCAGCAAGAUCAAGGUGA 22 13761

HSV2-UL30-5408 - GAAGCGCAGCAAGAUCAAGGUGA 23 13762

HSV2-UL30-5409 - AGAAGCGCAGCAAGAUCAAGGUGA 24 13763

HSV2-UL30-5410 - CUCCAUGCGCAUCAUCUA 18 13764

HSV2-UL30-5411 - ACUCCAUGCGCAUCAUCUA 19 13765

HSV2-UL30-664 - UACUCCAUGCGCAUCAUCUA 20 3534

HSV2-UL30-5412 - GUACUCCAUGCGCAUCAUCUA 21 13766

HSV2-UL30-5413 - CGUACUCCAUGCGCAUCAUCUA 22 13767

HSV2-UL30-5414 - CCGUACUCCAUGCGCAUCAUCUA 23 13768

HSV2-UL30-5415 - UCCGUACUCCAUGCGCAUCAUCUA 24 13769

HSV2-UL30-5416 - GCCCAG ACCCGCG AGG U A 18 13770

HSV2-UL30-5417 - GGCCCAGACCCGCGAGGUA 19 13771

HSV2-UL30-2020 - UGGCCCAG ACCCGCG AGG U A 20 4919

HSV2-UL30-5418 - GUGGCCCAG ACCCGCG AGGU A 21 13772

HSV2-UL30-5419 - CG UGGCCCAG ACCCGCG AGG U A 22 13773

HSV2-UL30-5420 - UCGUGGCCCAGACCCGCGAGGUA 23 13774

HSV2-UL30-5421 - AUCGUGGCCCAGACCCGCGAGG UA 24 13775

HSV2-UL30-5422 - AUCACCGAGAGUCUGU UA 18 13776

HSV2-UL30-5423 - GAUCACCGAGAGUCUGU UA 19 13777

HSV2-UL30-2048 - AGAUCACCGAGAGUCUG UUA 20 4935

HSV2-UL30-5424 - AAGAUCACCGAGAGUCUGUUA 21 13778

HSV2-UL30-5425 - CAAGAUCACCGAGAGUCUGUUA 22 13779

HSV2-UL30-5426 - CCAAGAUCACCGAGAGUCUGUUA 23 13780

HSV2-UL30-5427 - GCCAAGAUCACCGAGAGUCUGUUA 24 13781

HSV2-UL30-5428 - CGG UUUAUCCUGGACAAC 18 13782

HSV2-UL30-5429 - CCGGUU UAUCCUGGACAAC 19 13783

HSV2-UL30-1821 - CCCGGUUUAUCCUGGACAAC 20 4789

HSV2-UL30-5430 - ACCCGGUUUAUCCUGGACAAC 21 13784

HSV2-UL30-5431 - CACCCGGU UUAUCCUGGACAAC 22 13785

HSV2-UL30-5432 - CCACCCGGUUUAUCCUGGACAAC 23 13786

HSV2-UL30-5433 - ACCACCCGGUU UAUCCUGGACAAC 24 13787

HSV2-UL30-5434 - CGGGGCGUUCCGCUCAAC 18 13788 HSV2-UL30-5435 - CCGGGGCG UUCCGCUCAAC 19 13789

HSV2-UL30-2040 - CCCGGGGCGUUCCGCUCAAC 20 4930

HSV2-UL30-5436 - GCCCGGGGCGUUCCGCUCAAC 21 13790

HSV2-UL30-5437 - CGCCCGGGGCGUUCCGCUCAAC 22 13791

HSV2-UL30-5438 - UCGCCCGGGGCGU UCCGCU C A AC 23 13792

HSV2-UL30-5439 - AUCGCCCGGGGCGU UCCGCUCAAC 24 13793

HSV2-UL30-5440 - CGGCCGGGGGACGGGAAC 18 13794

HSV2-UL30-5441 - GCGGCCGGGGGACGGGAAC 19 13795

HSV2-UL30-590 - AGCGGCCGGGGGACGGGAAC 20 3460

HSV2-UL30-5442 - GAGCGGCCGGGGGACGGGAAC 21 13796

HSV2-UL30-5443 - GGAGCGGCCGGGGGACGGGAAC 22 13797

HSV2-UL30-5444 - GGG AGCGGCCGGGGGACGGGAAC 23 13798

HSV2-UL30-5445 - GGGGAGCGGCCGGGGGACGGGAAC 24 13799

HSV2-UL30-5446 - UUCAAGUUUCUGCCGCAC 18 13800

HSV2-UL30-5447 - CUUCAAGU UUCUGCCGCAC 19 13801

HSV2-UL30-1889 - UCUUCAAGUUUCUGCCGCAC 20 4836

HSV2-UL30-5448 - UUCUUCAAGUUUCUGCCGCAC 21 13802

HSV2-UL30-5449 - GU UCUUCAAGUUUCUGCCGCAC 22 13803

HSV2-UL30-5450 - UGUUCUUCAAGUUUCUGCCGCAC 23 13804

HSV2-UL30-5451 - CUGUUCU UCAAGU UUCUGCCGCAC 24 13805

HSV2-UL30-5452 - CCCGAGGCCGUCGCGCAC 18 13806

HSV2-UL30-5453 - GCCCGAGGCCGUCGCGCAC 19 13807

HSV2-UL30-1950 - GGCCCGAGGCCGUCGCGCAC 20 4869

HSV2-UL30-5454 - CGGCCCGAGGCCGUCGCGCAC 21 13808

HSV2-UL30-5455 - GCGGCCCGAGGCCGUCGCGCAC 22 13809

HSV2-UL30-5456 - UGCGGCCCGAGGCCGUCGCGCAC 23 13810

HSV2-UL30-5457 - CUGCGGCCCGAGGCCGUCGCGCAC 24 13811

HSV2-UL30-5458 - GGAGGUCGCGCGCGAGAC 18 13812

HSV2-UL30-5459 - AGGAGGUCGCGCGCGAGAC 19 13813

HSV2-UL30-604 - GAGGAGGUCGCGCGCGAGAC 20 3474

HSV2-UL30-5460 - CG AGGAGGUCGCGCGCGAGAC 21 13814

HSV2-UL30-5461 - GCG AGGAGGUCGCGCGCGAGAC 22 13815

HSV2-UL30-5462 - CGCG AGGAGGUCGCGCGCGAGAC 23 13816

HSV2-UL30-5463 - GCGCGAGGAGGUCGCGCGCGAGAC 24 13817

HSV2-UL30-5464 - GAGGAUAAGGACGACGAC 18 13818

HSV2-UL30-5465 - CGAGGAUAAGGACGACGAC 19 13819

HSV2-UL30-1926 - ACGAGGAUAAGGACGACGAC 20 4854

HSV2-UL30-5466 - GACGAGGAUAAGGACGACGAC 21 13820

HSV2-UL30-5467 - GG ACGAGGAUAAGGACGACGAC 22 13821

HSV2-UL30-5468 - GGG ACGAGGAUAAGGACGACGAC 23 13822

HSV2-UL30-5469 - GGGGACGAGGAUAAGGACGACGAC 24 13823

HSV2-UL30-5470 - CAUCGGCGCAUCACCGAC 18 13824

HSV2-UL30-5471 - CCAUCGGCGCAUCACCGAC 19 13825

HSV2-UL30-2008 - CCCAUCGGCGCAUCACCGAC 20 4909

HSV2-UL30-5472 - GCCCAUCGGCGCAUCACCGAC 21 13826

HSV2-UL30-5473 - CGCCCAUCGGCGCAUCACCGAC 22 13827 HSV2-UL30-5474 - ACGCCCAUCGGCGCAUCACCGAC 23 13828

HSV2-UL30-5475 - GACGCCCAUCGGCGCAUCACCGAC 24 13829

HSV2-UL30-5476 - CGGUUUCGGGGCCUCGAC 18 13830

HSV2-UL30-5477 - GCGGUUUCGGGGCCUCGAC 19 13831

HSV2-UL30-1900 - GGCGGUUUCGGGGCCUCGAC 20 4844

HSV2-UL30-5478 - GGGCGGU UUCGGGGCCUCGAC 21 13832

HSV2-UL30-5479 - GGGGCGGUU UCGGGGCCUCGAC 22 13833

HSV2-UL30-5480 - AGGGGCGGUUUCGGGGCCUCGAC 23 13834

HSV2-UL30-5481 - CAGGGGCGGUU UCGGGGCCUCGAC 24 13835

HSV2-UL30-5482 - GAGGCCGUCUUGAAGGAC 18 13836

HSV2-UL30-5483 - CGAGGCCGUCUUGAAGGAC 19 13837

HSV2-UL30-1879 - CCGAGGCCGUCUUGAAGGAC 20 4827

HSV2-UL30-5484 - GCCGAGGCCGUCUUGAAGGAC 21 13838

HSV2-UL30-5485 - CGCCGAGGCCGUCUUGAAGGAC 22 13839

HSV2-UL30-5486 - UCGCCGAGGCCGUCUUGAAGGAC 23 13840

HSV2-UL30-5487 - GUCGCCGAGGCCGUCUUGAAGGAC 24 13841

HSV2-UL30-5488 - AAGGACGACGACGAGGAC 18 13842

HSV2-UL30-5489 - UAAGGACGACGACGAGGAC 19 13843

HSV2-UL30-596 - AU AAGGACGACGACGAGGAC 20 3466

HSV2-UL30-5490 - GAU AAGGACGACGACGAGGAC 21 13844

HSV2-UL30-5491 - GGAU AAGGACGACGACGAGGAC 22 13845

HSV2-UL30-5492 - AGGAU AAGGACGACGACGAGGAC 23 13846

HSV2-UL30-5493 - GAGGAUAAGGACGACGACGAGGAC 24 13847

HSV2-UL30-5494 - GAGGACGGGGACGAGGAC 18 13848

HSV2-UL30-5495 - CGAGGACGGGGACGAGGAC 19 13849

HSV2-UL30-600 - ACGAGGACGGGGACGAGGAC 20 3470

HSV2-UL30-5496 - GACGAGGACGGGGACGAGGAC 21 13850

HSV2-UL30-5497 - CGACGAGGACGGGGACGAGGAC 22 13851

HSV2-UL30-5498 - ACG ACGAGGACGGGGACGAGGAC 23 13852

HSV2-UL30-5499 - GACGACGAGGACGGGGACGAGGAC 24 13853

HSV2-UL30-5500 - GCGCACCUGGAGGCGGAC 18 13854

HSV2-UL30-5501 - CGCGCACCUGGAGGCGGAC 19 13855

HSV2-UL30-627 - UCGCGCACCUGGAGGCGGAC 20 3497

HSV2-UL30-5502 - GUCGCGCACCUGGAGGCGGAC 21 13856

HSV2-UL30-5503 - CGUCGCGCACCUGGAGGCGGAC 22 13857

HSV2-UL30-5504 - CCGUCGCGCACCUGGAGGCGGAC 23 13858

HSV2-UL30-5505 - GCCGUCGCGCACCUGGAGGCGGAC 24 13859

HSV2-UL30-5506 - GACGACGAGGACGGGGAC 18 13860

HSV2-UL30-5507 - CGACGACGAGGACGGGGAC 19 13861

HSV2-UL30-1931 - ACGACGACGAGGACGGGGAC 20 4857

HSV2-UL30-5508 - GACGACGACGAGGACGGGGAC 21 13862

HSV2-UL30-5509 - GG ACGACGACGAGGACGGGGAC 22 13863

HSV2-UL30-5510 - AGG ACGACGACGAGGACGGGGAC 23 13864

HSV2-UL30-5511 - AAGG ACGACGACGAGGACGGGGAC 24 13865

HSV2-UL30-5512 - CGCAUCAUCUACGGGGAC 18 13866

HSV2-UL30-5513 - GCGCAUCAUCUACGGGGAC 19 13867 HSV2-UL30-1984 - UGCGCAUCAUCUACGGGGAC 20 4891

HSV2-UL30-5514 - AUGCGCAUCAUCUACGGGGAC 21 13868

HSV2-UL30-5515 - CAUGCGCAUCAUCUACGGGGAC 22 13869

HSV2-UL30-5516 - CCAUGCGCAUCAUCUACGGGGAC 23 13870

HSV2-UL30-5517 - UCCAUGCGCAUCAUCUACGGGGAC 24 13871

HSV2-UL30-5518 - GGGGAGCGGCCGGGGGAC 18 13872

HSV2-UL30-5519 - AGGGGAGCGGCCGGGGGAC 19 13873

HSV2-UL30-588 - AAGGGGAGCGGCCGGGGGAC 20 3458

HSV2-UL30-5520 - GAAGGGGAGCGGCCGGGGGAC 21 13874

HSV2-UL30-5521 - GGAAGGGGAGCGGCCGGGGGAC 22 13875

HSV2-UL30-5522 - GGG AAGGGGAGCGGCCGGGGGAC 23 13876

HSV2-UL30-5523 - GGGGAAGGGGAGCGGCCGGGGGAC 24

13877

HSV2-UL30-5524 - GAGGCGGACCGGGACUAC 18 13878

HSV2-UL30-5525 - GGAGGCGGACCGGGACUAC 19 13879

HSV2-UL30-1955 - UGGAGGCGGACCGGGACUAC 20 4872

HSV2-UL30-5526 - CUGGAGGCGGACCGGGACUAC 21 13880

HSV2-UL30-5527 - CCUGGAGGCGGACCGGGACUAC 22 13881

HSV2-UL30-5528 - ACCUGGAGGCGGACCGGGACUAC 23 13882

HSV2-UL30-5529 - CACCUGGAGGCGGACCGGGACUAC 24 13883

HSV2-UL30-5530 - UCCAUGCGCAUCAUCUAC 18 13884

HSV2-UL30-5531 - CUCCAUGCGCAUCAUCUAC 19 13885

HSV2-UL30-665 - ACUCCAUGCGCAUCAUCUAC 20 3535

HSV2-UL30-5532 - UACUCCAUGCGCAUCAUCUAC 21 13886

HSV2-UL30-5533 - GUACUCCAUGCGCAUCAUCUAC 22 13887

HSV2-UL30-5534 - CGUACUCCAUGCGCAUCAUCUAC 23 13888

HSV2-UL30-5535 - CCGUACUCCAUGCGCAUCAUCUAC 24 13889

HSV2-UL30-5536 - CCCGCGAUCAGGAAGUAC 18 13890

HSV2-UL30-5537 - CCCCGCGAUCAGGAAGUAC 19 13891

HSV2-UL30-1816 - GCCCCGCGAUCAGGAAGUAC 20 4787

HSV2-UL30-5538 - UGCCCCGCGAUCAGGAAGUAC 21 13892

HSV2-UL30-5539 - UUGCCCCGCGAUCAGGAAGUAC 22 13893

HSV2-UL30-5540 - UUUGCCCCGCGAUCAGGAAGUAC 23 13894

HSV2-UL30-5541 - U UUUGCCCCGCGAUCAGGAAGUAC 24 13895

HSV2-UL30-5542 - UCGUGAAGGCCCACGUAC 18 13896

HSV2-UL30-5543 - UUCGUGAAGGCCCACGUAC 19 13897

HSV2-UL30-1963 - CUUCGUGAAGGCCCACG UAC 20 4876

HSV2-UL30-5544 - UCUUCGUGAAGGCCCACGUAC 21 13898

HSV2-UL30-5545 - UUCU UCGUGAAGGCCCACGUAC 22 13899

HSV2-UL30-5546 - GUUCUUCGUGAAGGCCCACGUAC 23 13900

HSV2-UL30-5547 - UGUUCUUCGUGAAGGCCCACGUAC 24 13901

HSV2-UL30-5548 - GGCCGGCACGUUGGGUAC 18 13902

HSV2-UL30-5549 - GGGCCGGCACGUUGGG UAC 19 13903

HSV2-UL30-1944 - GGGGCCGGCACGU UGGGUAC 20 4865

HSV2-UL30-5550 - GGGGGCCGGCACGUUGGGUAC 21 13904

HSV2-UL30-5551 - CGGGGGCCGGCACGU UGGGUAC 22 13905 HSV2-UL30-5552 - CCGGGGGCCGGCACGUUGGGUAC 23 13906

HSV2-UL30-5553 - ACCGGGGGCCGGCACGUUGGGUAC 24 13907

HSV2-UL30-5554 - ACGCGGCAGUACUU UUAC 18 13908

HSV2-UL30-5555 - CACGCGGCAG UACUU UUAC 19 13909

HSV2-UL30-1795 - GCACGCGGCAGUACUUUUAC 20 4772

HSV2-UL30-5556 - GGCACGCGGCAGUACUU UUAC 21 13910

HSV2-UL30-5557 - CGGCACGCGGCAGUACU UUUAC 22 13911

HSV2-UL30-5558 - ACGGCACGCGGCAGUACUUUUAC 23 13912

HSV2-UL30-5559 - UACGGCACGCGGCAGUACUUUUAC 24 13913

HSV2-UL30-5560 - CCGACGGCGUUCGGAACC 18 13914

HSV2-UL30-5561 - CCCGACGGCGU UCGGAACC 19 13915

HSV2-UL30-1828 - CCCCGACGGCGUUCGGAACC 20 4792

HSV2-UL30-5562 - CCCCCGACGGCGUUCGGAACC 21 13916

HSV2-UL30-5563 - CCCCCCGACGGCGUUCGGAACC 22 13917

HSV2-UL30-5564 - GCCCCCCGACGGCGUUCGGAACC 23 13918

HSV2-UL30-5565 - CGCCCCCCGACGGCGU UCGGAACC 24 13919

HSV2-UL30-5566 - UUCAUCCUGCCGGACACC 18 13920

HSV2-UL30-5567 - CUUCAUCCUGCCGGACACC 19 13921

HSV2-UL30-1896 - GCUUCAUCCUGCCGGACACC 20 4842

HSV2-UL30-5568 - GGCUUCAUCCUGCCGGACACC 21 13922

HSV2-UL30-5569 - GGGCU UCAUCCUGCCGGACACC 22 13923

HSV2-UL30-5570 - AGGGCUUCAUCCUGCCGGACACC 23 13924

HSV2-UL30-5571 - AAGGGCU UCAUCCUGCCGGACACC 24 13925

HSV2-UL30-5572 - U C A AG U UUCUGCCGCACC 18 13926

HSV2-UL30-5573 - U U C A AG UUUCUGCCGCACC 19 13927

HSV2-UL30-554 - CUUCAAGUU UCUGCCGCACC 20 3424

HSV2-UL30-5574 - UCUUCAAGUU UCUGCCGCACC 21 13928

HSV2-UL30-5575 - UUCUUCAAGUU UCUGCCGCACC 22 13929

HSV2-UL30-5576 - GUUCUUCAAGU UUCUGCCGCACC 23 13930

HSV2-UL30-5577 - UGUUCUUCAAG UUUCUGCCGCACC 24 13931

HSV2-UL30-5578 - CCGAGGCCGUCGCGCACC 18 13932

HSV2-UL30-5579 - CCCGAGGCCGUCGCGCACC 19 13933

HSV2-UL30-624 - GCCCGAGGCCGUCGCGCACC 20 3494

HSV2-UL30-5580 - GGCCCGAGGCCGUCGCGCACC 21 13934

HSV2-UL30-5581 - CGGCCCG AGGCCG UCGCGCACC 22 13935

HSV2-UL30-5582 - G CG G CCCG AG G CCG U CG CG C ACC 23 13936

HSV2-UL30-5583 - UGCGGCCCGAGGCCGUCGCGCACC 24 13937

HSV2-UL30-5584 - GAGGUCGCGCGCGAGACC 18 13938

HSV2-UL30-5585 - GGAGGUCGCGCGCGAGACC 19 13939

HSV2-UL30-605 - AGGAGGUCGCGCGCGAGACC 20 3475

HSV2-UL30-5586 - GAGGAGGUCGCGCGCGAGACC 21 13940

HSV2-UL30-5587 - CG AGGAGGUCGCGCGCGAGACC 22 13941

HSV2-UL30-5588 - GCGAGGAGGUCGCGCGCGAGACC 23 13942

HSV2-UL30-5589 - CGCGAGGAGGUCGCGCGCGAGACC 24 13943

HSV2-UL30-5590 - AUCGGCGCAUCACCGACC 18 13944

HSV2-UL30-5591 - CAUCGGCGCAUCACCGACC 19 13945 HSV2-UL30-701 - CCAUCGGCGCAUCACCGACC 20 3571

HSV2-UL30-5592 - CCCAUCGGCGCAUCACCGACC 21 13946

HSV2-UL30-5593 - GCCCAUCGGCGCAUCACCGACC 22 13947

HSV2-UL30-5594 - CGCCCAUCGGCGCAUCACCGACC 23 13948

HSV2-UL30-5595 - ACGCCCAUCGGCGCAUCACCGACC 24 13949

HSV2-UL30-5596 - AGGCGGACCGGGACUACC 18 13950

HSV2-UL30-5597 - GAGGCGGACCGGGACUACC 19 13951

HSV2-UL30-629 - GG AGGCGGACCGGGACUACC 20 3499

HSV2-UL30-5598 - UGG AGGCGGACCGGGACUACC 21 13952

HSV2-UL30-5599 - CUGG AGGCGGACCGGGACUACC 22 13953

HSV2-UL30-5600 - CCUGG AGGCGGACCGGGACUACC 23 13954

HSV2-UL30-5601 - ACCUGGAGGCGGACCGGGACUACC 24 13955

HSV2-UL30-5602 - GCCGGCACGU UGGGUACC 18 13956

HSV2-UL30-5603 - GGCCGGCACGU UGGGUACC 19 13957

HSV2-UL30-611 - GGGCCGGCACGUUGGGUACC 20 3481

HSV2-UL30-5604 - GGGGCCGGCACGUUGGGUACC 21 13958

HSV2-UL30-5605 - GGGGGCCGGCACGUUGGGUACC 22 13959

HSV2-UL30-5606 - CGGGGGCCGGCACGUUGGGUACC 23 13960

HSV2-UL30-5607 - CCGGGGGCCGGCACGUUGGGUACC 24 13961

HSV2-UL30-5608 - UCAUCCUGCCGGACACCC 18 13962

HSV2-UL30-5609 - UUCAUCCUGCCGGACACCC 19 13963

HSV2-UL30-564 - CU UCAUCCUGCCGGACACCC 20 3434

HSV2-UL30-5610 - GCUUCAUCCUGCCGGACACCC 21 13964

HSV2-UL30-5611 - GGCUUCAUCCUGCCGGACACCC 22 13965

HSV2-UL30-5612 - GGGCUUCAUCCUGCCGGACACCC 23 13966

HSV2-UL30-5613 - AGGGCUUCAUCCUGCCGGACACCC 24 13967

HSV2-UL30-5614 - G G AU CCCCCAG AG C ACCC 18 13968

HSV2-UL30-5615 - CGGAUCCCCCAGAGCACCC 19 13969

HSV2-UL30-1969 - G CG G A U CCCCCAG AG CACCC 20 4882

HSV2-UL30-5616 - CGCGGAUCCCCCAGAGCACCC 21 13970

HSV2-UL30-5617 - U CG CG G AU CCCCCAG AG CACCC 22 13971

HSV2-UL30-5618 - CUCGCGGAUCCCCCAGAGCACCC 23 13972

HSV2-UL30-5619 - GCUCGCGGAUCCCCCAGAGCACCC 24 13973

HSV2-UL30-5620 - UGAUCGUGGCCCAGACCC 18 13974

HSV2-UL30-5621 - GUGAUCG UGGCCCAGACCC 19 13975

HSV2-UL30-2018 - CGUGAUCGUGG CCCAG ACCC 20 4917

HSV2-UL30-5622 - ACGUGAUCGUGGCCCAGACCC 21 13976

HSV2-UL30-5623 - UACGUGAUCGUGGCCCAGACCC 22 13977

HSV2-UL30-5624 - GUACGUGAUCGUGGCCCAGACCC 23 13978

HSV2-UL30-5625 - CGUACGUGAUCGUGGCCCAGACCC 24 13979

HSV2-UL30-5626 - CGCUUCUGGGUCUGACCC 18 13980

HSV2-UL30-5627 - ACGCU UCUGGGUCUGACCC 19 13981

HSV2-UL30-1794 - CACGCUUCUGGGUCUGACCC 20 4771

HSV2-UL30-5628 - UCACGCUUCUGGGUCUGACCC 21 13982

HSV2-UL30-5629 - AUCACGCUUCUGGGUCUGACCC 22 13983

HSV2-UL30-5630 - CAUCACGCU UCUGGGUCUGACCC 23 13984 HSV2-UL30-5631 - UCAUCACGCUUCUGGGUCUGACCC 24 13985

HSV2-UL30-5632 - CCCGAGACGUGGCACCCC 18 13986

HSV2-UL30-5633 - UCCCGAGACGUGGCACCCC 19 13987

HSV2-UL30-2050 - UUCCCGAGACGUGGCACCCC 20 4937

HSV2-UL30-5634 - AUUCCCGAGACGUGGCACCCC 21 13988

HSV2-UL30-5635 - UAUUCCCGAGACGUGGCACCCC 22 13989

HSV2-UL30-5636 - UU AU UCCCGAGACGUGGCACCCC 23 13990

HSV2-UL30-5637 - UUUAU UCCCGAGACGUGGCACCCC 24 13991

HSV2-UL30-5638 - CCGUCGCAUGCCGACCCC 18 13992

HSV2-UL30-5639 - GCCGUCGCAUGCCGACCCC 19 13993

HSV2-UL30-2030 - CGCCGUCGCAUGCCGACCCC 20 4924

HSV2-UL30-5640 - ACGCCGUCGCAUGCCGACCCC 21 13994

HSV2-UL30-5641 - GACG CCG U CG CAU G CCG ACCCC 22 13995

HSV2-UL30-5642 - AGACGCCGUCGCAUGCCGACCCC 23 13996

HSV2-UL30-5643 - GAGACGCCGUCGCAUGCCGACCCC 24 13997

HSV2-UL30-5644 - AUCCCCCAGAGCACCCCC 18 13998

HSV2-UL30-5645 - GAUCCCCCAGAGCACCCCC 19 13999

HSV2-UL30-1970 - GGAUCCCCCAGAGCACCCCC 20 4883

HSV2-UL30-5646 - CGGAUCCCCCAGAGCACCCCC 21 14000

HSV2-UL30-5647 - G CG G A U CCCCCAG AG CACCCCC 22 14001

HSV2-UL30-5648 - CGCGGAUCCCCCAGAGCACCCCC 23 14002

HSV2-UL30-5649 - U CG CG G AU CCCCCAG AG CACCCCC 24 14003

HSV2-UL30-5650 - CGUCGCAUGCCGACCCCC 18 14004

HSV2-UL30-5651 - CCGUCGCAUGCCGACCCCC 19 14005

HSV2-UL30-724 - GCCGUCGCAUGCCGACCCCC 20 3594

HSV2-UL30-5652 - CGCCGUCGCAUGCCGACCCCC 21 14006

HSV2-UL30-5653 - ACGCCGUCGCAUGCCGACCCCC 22 14007

HSV2-UL30-5654 - G ACGCCG UCGCAUGCCG ACCCCC 23 14008

HSV2-UL30-5655 - AGACGCCGUCGCAUGCCGACCCCC 24 14009

HSV2-UL30-5656 - G UCGCAUGCCG ACCCCCC 18 14010

HSV2-UL30-5657 - CG UCGCAUGCCG ACCCCCC 19 14011

HSV2-UL30-725 - CCG UCGCAUGCCG ACCCCCC 20 3595

HSV2-UL30-5658 - GCCGUCGCAUGCCGACCCCCC 21 14012

HSV2-UL30-5659 - CGCCGUCGCAUGCCGACCCCCC 22 14013

HSV2-UL30-5660 - ACGCCGUCGCAUGCCGACCCCCC 23 14014

HSV2-UL30-5661 - GACGCCG UCGCAUGCCG ACCCCCC 24 14015

HSV2-UL30-5662 - CCCCG G CCA AG CG CCCCC 18 14016

HSV2-UL30-5663 - UCCCCGGCCAAGCGCCCCC 19 14017

HSV2-UL30-723 - CUCCCCGGCCAAGCGCCCCC 20 3593

HSV2-UL30-5664 - CCUCCCCGGCCAAGCGCCCCC 21 14018

HSV2-UL30-5665 - CCCUCCCCGG CCA AG CG CCCCC 22 14019

HSV2-UL30-5666 - GCCCUCCCCGGCCAAGCGCCCCC 23 14020

HSV2-UL30-5667 - U G CCC U CCCCG G CCA AG CG CCCCC 24 14021

HSV2-UL30-5668 - GCUAGACGCCGCCGCCCC 18 14022

HSV2-UL30-5669 - AGCUAGACGCCGCCGCCCC 19 14023

HSV2-UL30-717 - GAGCUAGACGCCGCCGCCCC 20 3587 HSV2-UL30-5670 - CGAGCUAGACGCCGCCGCCCC 21 14024

HSV2-UL30-5671 - GCGAGCUAGACGCCGCCGCCCC 22 14025

HSV2-UL30-5672 - CGCGAGCUAGACGCCGCCGCCCC 23 14026

HSV2-UL30-5673 - CCGCGAGCUAGACGCCGCCGCCCC 24 14027

HSV2-UL30-5674 - UCCCCGG CCA AG CG CCCC 18 14028

HSV2-UL30-5675 - CUCCCCGGCCAAGCGCCCC 19 14029

HSV2-UL30-722 - CCU CCCCG G CCAAG CG CCCC 20 3592

HSV2-UL30-5676 - CCCUCCCCGGCCAAGCGCCCC 21 14030

HSV2-UL30-5677 - GCCCUCCCCGGCCAAGCGCCCC 22 14031

HSV2-UL30-5678 - UGCCCUCCCCGGCCAAGCGCCCC 23 14032

HSV2-UL30-5679 - CUGCCCUCCCCGG CC A AG CG CCCC 24 14033

HSV2-UL30-5680 - AUCCGCUCGCGGAUCCCC 18 14034

HSV2-UL30-5681 - GAUCCGCUCGCGGAUCCCC 19 14035

HSV2-UL30-1968 - AGAUCCGCUCGCGGAUCCCC 20 4881

HSV2-UL30-5682 - CAGAUCCGCUCGCGGAUCCCC 21 14036

HSV2-UL30-5683 - GCAGAUCCGCUCGCGGAUCCCC 22 14037

HSV2-UL30-5684 - AGCAGAUCCGCUCGCGGAUCCCC 23 14038

HSV2-UL30-5685 - AAGCAGAUCCGCUCGCGGAUCCCC 24 14039

HSV2-UL30-5686 - ACCUGU CCACC ACCG CCC 18 14040

HSV2-UL30-5687 - GACCUGUCCACCACCGCCC 19 14041

HSV2-UL30-1849 - CG ACCU G U CCACCACCG CCC 20 4803

HSV2-UL30-5688 - ACGACCUGUCCACCACCGCCC 21 14042

HSV2-UL30-5689 - UACGACCUGUCCACCACCGCCC 22 14043

HSV2-UL30-5690 - CUACGACCUGUCCACCACCGCCC 23 14044

HSV2-UL30-5691 - UCUACGACCUGUCCACCACCGCCC 24 14045

HSV2-UL30-5692 - AGCUAGACGCCGCCGCCC 18 14046

HSV2-UL30-5693 - GAGCUAGACGCCGCCGCCC 19 14047

HSV2-UL30-2023 - CGAGCUAGACGCCGCCGCCC 20 4921

HSV2-UL30-5694 - GCGAGCUAGACGCCGCCGCCC 21 14048

HSV2-UL30-5695 - CGCGAGCUAGACGCCGCCGCCC 22 14049

HSV2-UL30-5696 - CCGCGAGCUAGACGCCGCCGCCC 23 14050

HSV2-UL30-5697 - UCCGCGAGCUAGACGCCGCCGCCC 24 14051

HSV2-UL30-5698 - CU CCCCG G CCAAG CG CCC 18 14052

HSV2-UL30-5699 - CCUCCCCGGCCAAGCGCCC 19 14053

HSV2-UL30-2027 - CCC UCCCCGG CCAAG CG CCC 20 4923

HSV2-UL30-5700 - GCCCUCCCCGG CCA AG CG CCC 21 14054

HSV2-UL30-5701 - UGCCCUCCCCGG CCAAG CG CCC 22 14055

HSV2-UL30-5702 - CU GCCCU CCCCGG CCAAGCGCCC 23 14056

HSV2-UL30-5703 - CCUGCCCUCCCCGGCCAAGCGCCC 24 14057

HSV2-UL30-5704 - CUGGCGCGACCCCUGCCC 18 14058

HSV2-UL30-5705 - GCUGGCGCGACCCCUGCCC 19 14059

HSV2-UL30-2004 - GGCUGGCGCGACCCCUGCCC 20 4907

HSV2-UL30-5706 - UGGCUGGCGCGACCCCUGCCC 21 14060

HSV2-UL30-5707 - GUGGCUGGCGCGACCCCUGCCC 22 14061

HSV2-UL30-5708 - AGUGGCUGGCGCGACCCCUGCCC 23 14062

HSV2-UL30-5709 - GAGUGGCUGGCGCGACCCCUGCCC 24 14063 HSV2-UL30-5710 - UUCCGGUCGCGGAACGCC 18 14064

HSV2-UL30-5711 - UUUCCGGUCGCGGAACGCC 19 14065

HSV2-UL30-510 - CUU UCCGGUCGCGGAACGCC 20 3380

HSV2-UL30-5712 - CCUUUCCGGUCGCGGAACGCC 21 14066

HSV2-UL30-5713 - GCCUUUCCGGUCGCGGAACGCC 22 14067

HSV2-UL30-5714 - GGCCUUUCCGGUCGCGGAACGCC 23 14068

HSV2-UL30-5715 - UGGCCUUUCCGGUCGCGGAACGCC 24 14069

HSV2-UL30-5716 - CCCGGGUACGCCAUCGCC 18 14070

HSV2-UL30-5717 - UCCCGGG UACGCCAUCGCC 19 14071

HSV2-UL30-733 - AUCCCGGGUACGCCAUCGCC 20 3603

HSV2-UL30-5718 - GAUCCCGGGUACGCCAUCGCC 21 14072

HSV2-UL30-5719 - GGAUCCCGGGUACGCCAUCGCC 22 14073

HSV2-UL30-5720 - AGGAUCCCGGGUACGCCAUCGCC 23 14074

HSV2-UL30-5721 - GAGGAUCCCGGGUACGCCAUCGCC 24 14075

HSV2-UL30-5722 - AAGCUGCUGCUCAUCGCC 18 14076

HSV2-UL30-5723 - CAAGCUGCUGCUCAUCGCC 19 14077

HSV2-UL30-1990 - CCAAGCUGCUGCUCAUCGCC 20 4897

HSV2-UL30-5724 - ACCAAGCUGCUGCUCAUCGCC 21 14078

HSV2-UL30-5725 - CACCAAGCUGCUGCUCAUCGCC 22 14079

HSV2-UL30-5726 - UCACCAAGCUGCUGCUCAUCGCC 23 14080

HSV2-UL30-5727 - UUCACCAAGCUGCUGCUCAUCGCC 24 14081

HSV2-UL30-5728 - GGCCCUGCGCGAGUCGCC 18 14082

HSV2-UL30-5729 - CGGCCCUGCGCGAGUCGCC 19 14083

HSV2-UL30-459 - GCGGCCCUGCGCGAGUCGCC 20 3329

HSV2-UL30-5730 - GGCGGCCCUGCGCGAGUCGCC 21 14084

HSV2-UL30-5731 - CGGCGGCCCUGCGCGAGUCGCC 22 14085

HSV2-UL30-5732 - GCGGCGGCCCUGCGCGAGUCGCC 23 14086

HSV2-UL30-5733 - GGCGGCGGCCCUGCGCGAGUCGCC 24 14087

HSV2-UL30-5734 - GAUAUCGAAUGCAAGGCC 18 14088

HSV2-UL30-5735 - CGAUAUCGAAUGCAAGGCC 19 14089

HSV2-UL30-501 - UCGAUAUCGAAUGCAAGGCC 20 3371

HSV2-UL30-5736 - U UCG AU AUCG AAUGCAAGGCC 21 14090

HSV2-UL30-5737 - CUUCGAUAUCGAAUGCAAGGCC 22 14091

HSV2-UL30-5738 - GCU UCGAUAUCGAAUGCAAGGCC 23 14092

HSV2-UL30-5739 - UGCUUCGAUAUCGAAUGCAAGGCC 24 14093

HSV2-UL30-5740 - UGCCGCGGCCUCACGGCC 18 14094

HSV2-UL30-5741 - GUGCCGCGGCCUCACGGCC 19 14095

HSV2-UL30-1985 - UGUGCCGCGGCCUCACGGCC 20 4892

HSV2-UL30-5742 - UUGUGCCGCGGCCUCACGGCC 21 14096

HSV2-UL30-5743 - UUUGUGCCGCGGCCUCACGGCC 22 14097

HSV2-UL30-5744 - UUUUGUGCCGCGGCCUCACGGCC 23 14098

HSV2-UL30-5745 - GUUU UGUGCCGCGGCCUCACGGCC 24 14099

HSV2-UL30-5746 - ACCGCCUCAAGCCCGGCC 18 14100

HSV2-UL30-5747 - UACCGCCUCAAGCCCGGCC 19 14101

HSV2-UL30-1823 - GU ACCGCCUCAAGCCCGGCC 20 4790

HSV2-UL30-5748 - GGUACCGCCUCAAGCCCGGCC 21 14102 HSV2-UL30-5749 - UGGUACCGCCUCAAGCCCGGCC 22 14103

HSV2-UL30-5750 - CUGGUACCGCCUCAAGCCCGGCC 23 14104

HSV2-UL30-5751 - GCUGGUACCGCCUCAAGCCCGGCC 24 14105

HSV2-UL30-5752 - GGGGGAAGGGGAGCGGCC 18 14106

HSV2-UL30-5753 - GGGGGGAAGGGGAGCGGCC 19 14107

HSV2-UL30-584 - CGGGGGGAAGGGGAGCGGCC 20 3454

HSV2-UL30-5754 - UCGGGGGGAAGGGGAGCGGCC 21 14108

HSV2-UL30-5755 - CUCGGGGGGAAGGGGAGCGGCC 22 14109

HSV2-UL30-5756 - CCUCGGGGGGAAGGGGAGCGGCC 23 14110

HSV2-UL30-5757 - GCCUCGGGGGGAAGGGGAGCGGCC 24 14111

HSV2-UL30-5758 - CCGUGACGACCAUCGGCC 18 14112

HSV2-UL30-5759 - ACCGUGACGACCAUCGGCC 19 14113

HSV2-UL30-1975 - CACCGUGACGACCAUCGGCC 20 4886

HSV2-UL30-5760 - CCACCGUGACGACCAUCGGCC 21 14114

HSV2-UL30-5761 - GCCACCG UGACG ACCAUCGGCC 22 14115

HSV2-UL30-5762 - CGCCACCGUGACGACCAUCGGCC 23 14116

HSV2-UL30-5763 - CCGCCACCGUGACGACCAUCGGCC 24 14117

HSV2-UL30-5764 - GCCGCGCGGCUCAGGGCC 18 14118

HSV2-UL30-5765 - GGCCGCGCGGCUCAGGGCC 19 14119

HSV2-UL30-2052 - UGGCCGCGCGGCUCAGGGCC 20 4939

HSV2-UL30-5766 - GUGGCCGCGCGGCUCAGGGCC 21 14120

HSV2-UL30-5767 - CGUGGCCGCGCGGCUCAGGGCC 22 14121

HSV2-UL30-5768 - ACGUGGCCGCGCGGCUCAGGGCC 23 14122

HSV2-UL30-5769 - GACGUGGCCGCGCGGCUCAGGGCC 24 14123

HSV2-UL30-5770 - GCCGCGGGCCUGGUGGCC 18 14124

HSV2-UL30-5771 - GGCCGCGGGCCUGGUGGCC 19 14125

HSV2-UL30-1986 - CGGCCGCGGGCCUGGUGGCC 20 4893

HSV2-UL30-5772 - ACGGCCGCGGGCCUGGUGGCC 21 14126

HSV2-UL30-5773 - CACGGCCGCGGGCCUGGUGGCC 22 14127

HSV2-UL30-5774 - UCACGGCCGCGGGCCUGGUGGCC 23 14128

HSV2-UL30-5775 - CUCACGGCCGCGGGCCUGGUGGCC 24 14129

HSV2-UL30-5776 - CA AG CG CCCG G CCG U G CC 18 14130

HSV2-UL30-5777 - CCA AG CGCCCGGCCGUGCC 19 14131

HSV2-UL30-1902 - CCCAAGCGCCCGGCCGUGCC 20 4845

HSV2-UL30-5778 - GCCCA AG CGCCCGGCCGUGCC 21 14132

HSV2-UL30-5779 - CG CCC A AG CGCCCGGCCGUGCC 22 14133

HSV2-UL30-5780 - GCGCCCAAGCGCCCGGCCGUGCC 23 14134

HSV2-UL30-5781 - GGCGCCCAAGCGCCCGGCCGUGCC 24 14135

HSV2-UL30-5782 - CGCGGCUGGCCGCCCUCC 18 14136

HSV2-UL30-5783 - GCGCGGCUGGCCGCCCUCC 19 14137

HSV2-UL30-2022 - CGCGCGGCUGGCCGCCCUCC 20 4920

HSV2-UL30-5784 - UCGCGCGGCUGGCCGCCCUCC 21 14138

HSV2-UL30-5785 - GUCGCGCGGCUGGCCGCCCUCC 22 14139

HSV2-UL30-5786 - GGUCGCGCGGCUGGCCGCCCUCC 23 14140

HSV2-UL30-5787 - CGGUCGCGCGGCUGGCCGCCCUCC 24 14141

HSV2-UL30-5788 - CUCAGCGAUCUCGCCUCC 18 14142 HSV2-UL30-5789 - CCUCAGCGAUCUCGCCUCC 19 14143

HSV2-UL30-512 - ACCUCAGCGAUCUCGCCUCC 20 3382

HSV2-UL30-5790 - CACCUCAGCGAUCUCGCCUCC 21 14144

HSV2-UL30-5791 - CCACCUCAGCGAUCUCGCCUCC 22 14145

HSV2-UL30-5792 - CCCACCUCAGCGAUCUCGCCUCC 23 14146

HSV2-UL30-5793 - UCCCACCUCAGCGAUCUCGCCUCC 24 14147

HSV2-UL30-3338 - UGCGCGCCGCCCAGCUCC 18 11692

HSV2-UL30-3339 - AUGCGCGCCGCCCAGCUCC 19 11693

HSV2-UL30-3340 - CAUGCGCGCCGCCCAGCUCC 20 11694

HSV2-UL30-3341 - GCAUGCGCGCCGCCCAGCUCC 21 11695

HSV2-UL30-3342 - AGCAUGCGCGCCGCCCAGCUCC 22 11696

HSV2-UL30-3343 - CAGCAUGCGCGCCGCCCAGCUCC 23 11697

HSV2-UL30-3344 - ACAGCAUGCGCGCCGCCCAGCUCC 24 11698

HSV2-UL30-5794 - UCGUUCCGCGGCAUCUCC 18 14148

HSV2-UL30-5795 - GUCGU UCCGCGGCAUCUCC 19 14149

HSV2-UL30-1804 - CGUCGUUCCGCGGCAUCUCC 20 4778

HSV2-UL30-5796 - GCGUCGUUCCGCGGCAUCUCC 21 14150

HSV2-UL30-5797 - GGCGUCGU UCCGCGGCAUCUCC 22 14151

HSV2-UL30-5798 - GGGCGUCGUUCCGCGGCAUCUCC 23 14152

HSV2-UL30-5799 - GGGGCGUCGU UCCGCGGCAUCUCC 24 14153

HSV2-UL30-5800 - CCUCCGGGCCCGCGCAGC 18 14154

HSV2-UL30-5801 - GCCUCCGGGCCCGCGCAGC 19 14155

HSV2-UL30-1884 - CGCCUCCGGGCCCGCGCAGC 20 4832

HSV2-UL30-5802 - ACGCCUCCGGGCCCGCGCAGC 21 14156

HSV2-UL30-5803 - UACGCCUCCGGGCCCGCGCAGC 22 14157

HSV2-UL30-5804 - CUACGCCUCCGGGCCCGCGCAGC 23 14158

HSV2-UL30-5805 - ACUACGCCUCCGGGCCCGCGCAGC 24 14159

HSV2-UL30-5806 - ACGAGGACGGGGACGAGC 18 14160

HSV2-UL30-5807 - GACGAGGACGGGGACGAGC 19 14161

HSV2-UL30-1936 - GG ACGAGGACGGGGACGAGC 20 4860

HSV2-UL30-5808 - GGG ACGAGGACGGGGACGAGC 21 14162

HSV2-UL30-5809 - GGGG ACGAGGACGGGGACGAGC 22 14163

HSV2-UL30-5810 - CGGGG ACGAGGACGGGGACGAGC 23 14164

HSV2-UL30-5811 - ACGGGGACGAGGACGGGGACGAGC 24 14165

HSV2-UL30-5812 - AGCCUUUGAUACUCUAGC 18 14166

HSV2-UL30-5813 - GAGCCUUUGAUACUCUAGC 19 14167

HSV2-UL30-5814 - AGAGCCUU UGAUACUCUAGC 20 14168

HSV2-UL30-5815 - UAGAGCCU UUGAUACUCUAGC 21 14169

HSV2-UL30-5816 - AUAGAGCCUUUGAUACUCUAGC 22 14170

HSV2-UL30-5817 - CAUAGAGCCUU UGAUACUCUAGC 23 14171

HSV2-UL30-5818 - GCAUAGAGCCU UUGAUACUCUAGC 24 14172

HSV2-UL30-5819 - UUUCCGGUCGCGGAACGC 18 14173

HSV2-UL30-5820 - CUUUCCGGUCGCGGAACGC 19 14174

HSV2-UL30-1847 - CCUU UCCGGUCGCGGAACGC 20 4802

HSV2-UL30-5821 - GCCUU UCCGGUCGCGGAACGC 21 14175

HSV2-UL30-5822 - GGCCU UUCCGGUCGCGGAACGC 22 14176 HSV2-UL30-5823 - UGGCCUUUCCGG UCGCGGAACGC 23 14177

HSV2-UL30-5824 - CUGGCCU UUCCGGUCGCGGAACGC 24 14178

HSV2-UL30-5825 - GUGAAGGCCCACGUACGC 18 14179

HSV2-UL30-5826 - CGUGAAGGCCCACGUACGC 19 14180

HSV2-UL30-1964 - UCGUGAAGGCCCACGUACGC 20 4877

HSV2-UL30-5827 - UUCGUGAAGGCCCACGUACGC 21 14181

HSV2-UL30-5828 - CU UCGUGAAGGCCCACGUACGC 22 14182

HSV2-UL30-5829 - UCUUCGUGAAGGCCCACGUACGC 23 14183

HSV2-UL30-5830 - UUCUUCGUGAAGGCCCACGUACGC 24 14184

HSV2-UL30-5831 - GGACGCCAUCACGCCCGC 18 14185

HSV2-UL30-5832 - UGGACGCCAUCACGCCCGC 19 14186

HSV2-UL30-443 - AUGGACGCCAUCACGCCCGC 20 3313

HSV2-UL30-5833 - UAUGGACGCCAUCACGCCCGC 21 14187

HSV2-UL30-5834 - UUAUGGACGCCAUCACGCCCGC 22 14188

HSV2-UL30-5835 - UUUAUGGACGCCAUCACGCCCGC 23 14189

HSV2-UL30-5836 - AUUUAUGGACGCCAUCACGCCCGC 24 14190

HSV2-UL30-5837 - CGCCUCAAGCCCGGCCGC 18 14191

HSV2-UL30-5838 - CCGCCUCAAGCCCGGCCGC 19 14192

HSV2-UL30-487 - ACCGCCUCAAGCCCGGCCGC 20 3357

HSV2-UL30-5839 - UACCGCCU C A AG CCCGGCCGC 21 14193

HSV2-UL30-5840 - GUACCGCCUCAAGCCCGGCCGC 22 14194

HSV2-UL30-5841 - GGUACCGCCUCAAGCCCGGCCGC 23 14195

HSV2-UL30-5842 - UGGUACCGCCUCAAGCCCGGCCGC 24 14196

HSV2-UL30-5843 - GAGGACGGGGACGAGCGC 18 14197

HSV2-UL30-5844 - CGAGGACGGGGACGAGCGC 19 14198

HSV2-UL30-1937 - ACGAGGACGGGGACGAGCGC 20 4861

HSV2-UL30-5845 - GACGAGGACGGGGACGAGCGC 21 14199

HSV2-UL30-5846 - GG ACGAGGACGGGGACGAGCGC 22 14200

HSV2-UL30-5847 - GGG ACGAGGACGGGGACGAGCGC 23 14201

HSV2-UL30-5848 - GGGGACGAGGACGGGGACGAGCGC 24 14202

HSV2-UL30-5849 - AGCGCGAGGAGGUCGCGC 18 14203

HSV2-UL30-5850 - GAGCGCGAGGAGGUCGCGC 19 14204

HSV2-UL30-1939 - CG AGCGCGAGGAGGUCGCGC 20 4862

HSV2-UL30-5851 - ACG AGCGCGAGGAGGUCGCGC 21 14205

HSV2-UL30-5852 - GACG AGCGCGAGGAGGUCGCGC 22 14206

HSV2-UL30-5853 - GGACG AGCGCGAGGAGGUCGCGC 23 14207

HSV2-UL30-5854 - GGGACGAGCGCGAGGAGGUCGCGC 24 14208

HSV2-UL30-5855 - CUCGACGGGUACGGGCGC 18 14209

HSV2-UL30-5856 - GCUCGACGGGUACGGGCGC 19 14210

HSV2-UL30-1864 - CGCUCGACGGGUACGGGCGC 20 4815

HSV2-UL30-5857 - CCGCUCGACGGGUACGGGCGC 21 14211

HSV2-UL30-5858 - CCCGCUCGACGGGUACGGGCGC 22 14212

HSV2-UL30-5859 - UCCCGCUCGACGGGUACGGGCGC 23 14213

HSV2-UL30-5860 - GUCCCGCUCGACGGGUACGGGCGC 24 14214

HSV2-UL30-5861 - UCCCGGGUACGCCAUCGC 18 14215

HSV2-UL30-5862 - AUCCCGGGUACGCCAUCGC 19 14216 HSV2-UL30-2038 - GAUCCCGGGUACGCCAUCGC 20 4929

HSV2-UL30-5863 - GGAUCCCGGGUACGCCAUCGC 21 14217

HSV2-UL30-5864 - AGGAUCCCGGGUACGCCAUCGC 22 14218

HSV2-UL30-5865 - GAGGAUCCCGGGUACGCCAUCGC 23 14219

HSV2-UL30-5866 - GG AGGAUCCCGGGUACGCCAUCGC 24 14220

HSV2-UL30-5867 - CGGCCCUGCGCGAGUCGC 18 14221

HSV2-UL30-5868 - GCGGCCCUGCGCGAGUCGC 19 14222

HSV2-UL30-458 - GGCGGCCCUGCGCGAGUCGC 20 3328

HSV2-UL30-5869 - CGGCGGCCCUGCGCG AG UCGC 21 14223

HSV2-UL30-5870 - GCGGCGGCCCUGCGCGAGUCGC 22 14224

HSV2-UL30-5871 - GGCGGCGGCCCUGCGCGAGUCGC 23 14225

HSV2-UL30-5872 - UGGCGGCGGCCCUGCGCGAGUCGC 24 14226

HSV2-UL30-5873 - CGAUAUCGAAUGCAAGGC 18 14227

HSV2-UL30-5874 - UCGAUAUCGAAUGCAAGGC 19 14228

HSV2-UL30-500 - UUCGAUAUCGAAUGCAAGGC 20 3370

HSV2-UL30-5875 - CU UCGAUAUCGAAUGCAAGGC 21 14229

HSV2-UL30-5876 - GCU UCGAUAUCGAAUGCAAGGC 22 14230

HSV2-UL30-5877 - UGCU UCGAUAUCGAAUGCAAGGC 23 14231

HSV2-UL30-5878 - GUGCUUCGAUAUCGAAUGCAAGGC 24 14232

HSV2-UL30-5879 - CGCGGGGUUCGGGCCGGC 18 14233

HSV2-UL30-5880 - CCGCGGGGUUCGGGCCGGC 19 14234

HSV2-UL30-758 - GCCGCGGGGU UCGGGCCGGC 20 3628

HSV2-UL30-5881 - GGCCGCGGGGU UCGGGCCGGC 21 14235

HSV2-UL30-5882 - GGGCCGCGGGGUUCGGGCCGGC 22 14236

HSV2-UL30-5883 - AGGGCCGCGGGGUUCGGGCCGGC 23 14237

HSV2-UL30-5884 - CAGGGCCGCGGGGU UCGGGCCGGC 24 14238

HSV2-UL30-5885 - GGGGGGAAGGGGAGCGGC 18 14239

HSV2-UL30-5886 - CGGGGGGAAGGGGAGCGGC 19 14240

HSV2-UL30-583 - UCGGGGGGAAGGGGAGCGGC 20 3453

HSV2-UL30-5887 - CUCGGGGGGAAGGGGAGCGGC 21 14241

HSV2-UL30-5888 - CCUCGGGGGGAAGGGGAGCGGC 22 14242

HSV2-UL30-5889 - GCCUCGGGGGGAAGGGGAGCGGC 23 14243

HSV2-UL30-5890 - UGCCUCGGGGGGAAGGGGAGCGGC 24 14244

HSV2-UL30-5891 - GUACGCUCUCCCUGCGGC 18 14245

HSV2-UL30-5892 - AGUACGCUCUCCCUGCGGC 19 14246

HSV2-UL30-1949 - CAGUACGCUCUCCCUGCGGC 20 4868

HSV2-UL30-5893 - UCAGUACGCUCUCCCUGCGGC 21 14247

HSV2-UL30-5894 - UUCAGUACGCUCUCCCUGCGGC 22 14248

HSV2-UL30-5895 - CU UCAGUACGCUCUCCCUGCGGC 23 14249

HSV2-UL30-5896 - GCUUCAGUACGCUCUCCCUGCGGC 24 14250

HSV2-UL30-5897 - CGCGUGUGGGACAUCGGC 18 14251

HSV2-UL30-5898 - CCGCGUG UGGGACAUCGGC 19 14252

HSV2-UL30-1869 - UCCGCGUGUGGGACAUCGGC 20 4818

HSV2-UL30-5899 - UUCCGCGUGUGGGACAUCGGC 21 14253

HSV2-UL30-5900 - GUUCCGCGUGUGGGACAUCGGC 22 14254

HSV2-UL30-5901 - UGUUCCGCGUGUGGGACAUCGGC 23 14255 HSV2-UL30-5902 - GUGUUCCGCGUGUGGGACAUCGGC 24 14256

HSV2-UL30-5903 - CUCCUGCGCCU UGCGGGC 18 14257

HSV2-UL30-5904 - CCUCCUGCGCCUUGCGGGC 19 14258

HSV2-UL30-1893 - GCCUCCUGCGCCUUGCGGGC 20 4839

HSV2-UL30-5905 - UGCCUCCUGCGCCU UGCGGGC 21 14259

HSV2-UL30-5906 - GUGCCUCCUGCGCCU UGCGGGC 22 14260

HSV2-UL30-5907 - CGUGCCUCCUGCGCCUUGCGGGC 23 14261

HSV2-UL30-5908 - ACGUGCCUCCUGCGCCUUGCGGGC 24 14262

HSV2-UL30-5909 - AUUACUUCUCGCACCUGC 18 14263

HSV2-UL30-5910 - UAU UACUUCUCGCACCUGC 19 14264

HSV2-UL30-737 - CUAUUACUUCUCGCACCUGC 20 3607

HSV2-UL30-5911 - ACUAU UACU UCUCGCACCUGC 21 14265

HSV2-UL30-5912 - GACUAU UACUUCUCGCACCUGC 22 14266

HSV2-UL30-5913 - GGACUAUUACUUCUCGCACCUGC 23 14267

HSV2-UL30-5914 - CGGACUAU UACU UCUCGCACCUGC 24 14268

HSV2-UL30-5915 - GGCUGGCGCGACCCCUGC 18 14269

HSV2-UL30-5916 - UGGCUGGCGCGACCCCUGC 19 14270

HSV2-UL30-2003 - GUGGCUGGCGCGACCCCUGC 20 4906

HSV2-UL30-5917 - AGUGGCUGGCGCGACCCCUGC 21 14271

HSV2-UL30-5918 - GAGUGGCUGGCGCGACCCCUGC 22 14272

HSV2-UL30-5919 - GGAG UGGCUGGCGCGACCCCUGC 23 14273

HSV2-UL30-5920 - AGGAGUGGCUGGCGCGACCCCUGC 24 14274

HSV2-UL30-5921 - UACAUCGGCGUCAUCUGC 18 14275

HSV2-UL30-5922 - GUACAUCGGCGUCAUCUGC 19 14276

HSV2-UL30-679 - AGUACAUCGGCGUCAUCUGC 20 3549

HSV2-UL30-5923 - AAGUACAUCGGCGUCAUCUGC 21 14277

HSV2-UL30-5924 - AAAGUACAUCGGCGUCAUCUGC 22 14278

HSV2-UL30-5925 - AAAAGUACAUCGGCGUCAUCUGC 23 14279

HSV2-UL30-5926 - AAAAAGUACAUCGGCGUCAUCUGC 24 14280

HSV2-UL30-5927 - GACCCGGAGAGGGACAUC 18 14281

HSV2-UL30-5928 - CGACCCGGAGAGGGACAUC 19 14282

HSV2-UL30-2013 - CCGACCCGGAGAGGGACAUC 20 4912

HSV2-UL30-5929 - ACCGACCCGGAGAGGGACAUC 21 14283

HSV2-UL30-5930 - C ACCG ACCCG G AG AG G G AC A U C 22 14284

HSV2-UL30-5931 - UCACCGACCCGGAGAGGGACAUC 23 14285

HSV2-UL30-5932 - AUCACCGACCCGGAGAGGGACAUC 24 14286

HSV2-UL30-5933 - GCGCAGGUCCCGUCCAUC 18 14287

HSV2-UL30-5934 - CGCGCAGGUCCCGUCCAUC 19 14288

HSV2-UL30-2016 - GCGCGCAGGUCCCGUCCAUC 20 4915

HSV2-UL30-5935 - CGCGCGCAGGUCCCGUCCAUC 21 14289

HSV2-UL30-5936 - CCGCGCGCAGGUCCCGUCCAUC 22 14290

HSV2-UL30-5937 - GCCGCGCGCAGGUCCCGUCCAUC 23 14291

HSV2-UL30-5938 - CG CCG CGCG CAG G U CCCG U CC AU C 24 14292

HSV2-UL30-5939 - GGGGGCAAGAUGCUCAUC 18 14293

HSV2-UL30-5940 - CGGGGGCAAGAUGCUCAUC 19 14294

HSV2-UL30-1994 - GCGGGGGCAAGAUGCUCAUC 20 4899 HSV2-UL30-5941 - UGCGGGGGCAAGAUGCUCAUC 21 14295

HSV2-UL30-5942 - CUGCGGGGGCAAGAUGCUCAUC 22 14296

HSV2-UL30-5943 - UCUGCGGGGGCAAGAUGCUCAUC 23 14297

HSV2-UL30-5944 - AUCUGCGGGGGCAAGAUGCUCAUC 24 14298

HSV2-UL30-5945 - AACUUU UGCCCCGCGAUC 18 14299

HSV2-UL30-5946 - CAACU UUUGCCCCGCGAUC 19 14300

HSV2-UL30-472 - ACAACU UUUGCCCCGCGAUC 20 3342

HSV2-UL30-5947 - GACAACUUU UGCCCCGCGAUC 21 14301

HSV2-UL30-5948 - CGACAACUU UUGCCCCGCGAUC 22 14302

HSV2-UL30-5949 - GCGACAACU UUUGCCCCGCGAUC 23 14303

HSV2-UL30-5950 - UGCGACAACUUU UGCCCCGCGAUC 24 14304

HSV2-UL30-5951 - UUACGACGAUACCGUAUC 18 14305

HSV2-UL30-5952 - UUUACGACGAUACCGUAUC 19 14306

HSV2-UL30-689 - UUUUACGACGAUACCGUAUC 20 3559

HSV2-UL30-5953 - GUUU UACGACGAUACCGUAUC 21 14307

HSV2-UL30-5954 - UGUUUUACGACGAUACCGUAUC 22 14308

HSV2-UL30-5955 - CU G U U U UACGACGAUACCG U AUC 23 14309

HSV2-UL30-5956 - GCUGUUUUACGACGAUACCGUAUC 24 14310

HSV2-UL30-5957 - GCCACCACCCGGU UUAUC 18 14311

HSV2-UL30-5958 - CGCCACCACCCGGUU UAUC 19 14312

HSV2-UL30-1820 - ACGCCACCACCCGGUUUAUC 20 4788

HSV2-UL30-5959 - GACGCCACCACCCGGUU UAUC 21 14313

HSV2-UL30-5960 - CG ACGCCACCACCCGG U U U AU C 22 14314

HSV2-UL30-5961 - UCGACGCCACCACCCGGU UUAUC 23 14315

HSV2-UL30-5962 - GUCGACGCCACCACCCGGU UUAUC 24 14316

HSV2-UL30-5963 - GUUUAUCAACCGCACCUC 18 14317

HSV2-UL30-5964 - CGU UUAUCAACCGCACCUC 19 14318

HSV2-UL30-1996 - GCGU UUAUCAACCGCACCUC 20 4901

HSV2-UL30-5965 - CGCGUUUAUCAACCGCACCUC 21 14319

HSV2-UL30-5966 - GCGCGU UUAUCAACCGCACCUC 22 14320

HSV2-UL30-5967 - UGCGCGUU UAUCAACCGCACCUC 23 14321

HSV2-UL30-5968 - CUGCGCGUUUAUCAACCGCACCUC 24 14322

HSV2-UL30-5969 - CCUCAGCGAUCUCGCCUC 18 14323

HSV2-UL30-5970 - ACCUCAGCGAUCUCGCCUC 19 14324

HSV2-UL30-1852 - CACCUCAGCGAUCUCGCCUC 20 4806

HSV2-UL30-5971 - CCACCUCAGCGAUCUCGCCUC 21 14325

HSV2-UL30-5972 - CCCACCUCAGCGAUCUCGCCUC 22 14326

HSV2-UL30-5973 - UCCCACCUCAGCGAUCUCGCCUC 23 14327

HSV2-UL30-5974 - GUCCCACCUCAGCGAUCUCGCCUC 24 14328

HSV2-UL30-5975 - AGCGCCCGGCCGUGCCUC 18 14329

HSV2-UL30-5976 - AAGCGCCCGGCCGUGCCUC 19 14330

HSV2-UL30-575 - CAAGCGCCCGGCCGUGCCUC 20 3445

HSV2-UL30-5977 - CCA AG CGCCCGGCCGUGCCUC 21 14331

HSV2-UL30-5978 - CCCAAGCGCCCGGCCGUGCCUC 22 14332

HSV2-UL30-5979 - GCCCAAGCGCCCGGCCG UGCCUC 23 14333

HSV2-UL30-5980 - CGCCCA AG CGCCCGGCCGUGCCUC 24 14334 HSV2-UL30-5981 - GCCGUCGCCGAGGCCGUC 18 14335

HSV2-UL30-5982 - CGCCGUCGCCGAGGCCGUC 19 14336

HSV2-UL30-1877 - ACGCCGUCGCCGAGGCCGUC 20 4825

HSV2-UL30-5983 - AACGCCG U CG CCG AG G CCG U C 21 14337

HSV2-UL30-5984 - GAACGCCGUCGCCGAGGCCGUC 22 14338

HSV2-UL30-5985 - UGAACGCCGUCGCCGAGGCCGUC 23 14339

HSV2-UL30-5986 - CUGAACGCCGUCGCCGAGGCCGUC 24 14340

HSV2-UL30-5987 - GCGGACAACCUGGCCGUC 18 14341

HSV2-UL30-5988 - GGCGGACAACCUGGCCGUC 19 14342

HSV2-UL30-1832 - CGGCGGACAACCUGGCCGUC 20 4796

HSV2-UL30-5989 - ACGGCGGACAACCUGGCCGUC 21 14343

HSV2-UL30-5990 - CACGGCGGACAACCUGGCCGUC 22 14344

HSV2-UL30-5991 - GCACGGCGGACAACCUGGCCGUC 23 14345

HSV2-UL30-5992 - UGCACGGCGGACAACCUGGCCGUC 24 14346

HSV2-UL30-5993 - CUGCCGGCCCCCGUCGUC 18 14347

HSV2-UL30-5994 - CCUGCCGGCCCCCGUCGUC 19 14348

HSV2-UL30-1854 - GCCUGCCGGCCCCCGUCGUC 20 4807

HSV2-UL30-5995 - GGCCUGCCGGCCCCCGUCGUC 21 14349

HSV2-UL30-5996 - GGGCCUGCCGGCCCCCG UCGUC 22 14350

HSV2-UL30-5997 - GGGGCCUGCCGGCCCCCGUCGUC 23 14351

HSV2-UL30-5998 - AGGGGCCUGCCGGCCCCCGUCGUC 24 14352

HSV2-UL30-5999 - GAGCUGGCCUU UCCGGUC 18 14353

HSV2-UL30-6000 - CGAGCUGGCCUUUCCGG UC 19 14354

HSV2-UL30-1845 - ACGAGCUGGCCUUUCCGGUC 20 4801

HSV2-UL30-6001 - GACGAGCUGGCCUUUCCGGUC 21 14355

HSV2-UL30-6002 - GGACGAGCUGGCCU UUCCGGUC 22 14356

HSV2-UL30-6003 - AGGACGAGCUGGCCU UUCCGGUC 23 14357

HSV2-UL30-6004 - GAGGACGAGCUGGCCUUUCCGGUC 24 14358

HSV2-UL30-6005 - UGUUAAAGAGGU UUAU UC 18 14359

HSV2-UL30-6006 - CUGUUAAAGAGGUUUAUUC 19 14360

HSV2-UL30-2049 - UCUGU UAAAGAGGUUUAUUC 20 4936

HSV2-UL30-6007 - GUCUGU UAAAGAGGUU UAUUC 21 14361

HSV2-UL30-6008 - AGUCUGUUAAAGAGGUUUAU UC 22 14362

HSV2-UL30-6009 - GAGUCUGUUAAAGAGGUUUAU UC 23 14363

HSV2-UL30-6010 - AGAGUCUGU UAAAGAGGUU UAUUC 24

14364

HSV2-UL30-6011 - GGCCGACGGCUGUUCU UC 18 14365

HSV2-UL30-6012 - GGGCCGACGGCUGUUCU UC 19 14366

HSV2-UL30-1962 - GGGGCCGACGGCUGUUCUUC 20 4875

HSV2-UL30-6013 - GGGGGCCGACGGCUGUUCUUC 21 14367

HSV2-UL30-6014 - GGGGGGCCGACGGCUGU UCUUC 22 14368

HSV2-UL30-6015 - UGGGGGGCCGACGGCUGU UCUUC 23 14369

HSV2-UL30-6016 - GUGGGGGGCCGACGGCUGUUCUUC 24 14370

HSV2-UL30-6017 - AGCUGCUGGCCGACUU UC 18 14371

HSV2-UL30-6018 - CAGCUGCUGGCCGACU UUC 19 14372

HSV2-UL30-659 - UCAGCUGCUGGCCGACU UUC 20 3529 HSV2-UL30-6019 - AUCAGCUGCUGGCCGACUUUC 21 14373

HSV2-UL30-6020 - GAUCAGCUGCUGGCCGACUUUC 22 14374

HSV2-UL30-6021 - CGAUCAGCUGCUGGCCGACU UUC 23 14375

HSV2-UL30-6022 - UCGAUCAGCUGCUGGCCGACU UUC 24 14376

HSV2-UL30-6023 - UACUU UUACAUGAACAAG 18 14377

HSV2-UL30-6024 - G U ACU U U U ACAU G AACAAG 19 14378

HSV2-UL30-1796 - AGUACU UUUACAUGAACAAG 20 4773

HSV2-UL30-6025 - CAGUACUUU UACAUG AACAAG 21 14379

HSV2-UL30-6026 - GCAG U ACU U U U ACAU G AACAAG 22 14380

HSV2-UL30-6027 - GGCAGUACU UUUACAUGAACAAG 23 14381

HSV2-UL30-6028 - CGGCAGUACUU UUACAUGAACAAG 24 14382

HSV2-UL30-6029 - GCCGUCU UGAAGGACAAG 18 14383

HSV2-UL30-6030 - GGCCGUCUUGAAGGACAAG 19 14384

HSV2-UL30-1880 - AGGCCGUCU UGAAGGACAAG 20 4828

HSV2-UL30-6031 - GAGGCCGUCUUGAAGGACAAG 21 14385

HSV2-UL30-6032 - CGAGGCCGUCUUGAAGGACAAG 22 14386

HSV2-UL30-6033 - CCGAGGCCGUCU UGAAGGACAAG 23 14387

HSV2-UL30-6034 - GCCGAGGCCGUCUUGAAGGACAAG 24 14388

HSV2-UL30-6035 - AAACUCUCCAGCUACAAG 18 14389

HSV2-UL30-6036 - CAAACUCUCCAGCUACAAG 19 14390

HSV2-UL30-1875 - UCAAACUCUCCAGCUACAAG 20 4823

HSV2-UL30-6037 - GUCAAACUCUCCAGCUACAAG 21 14391

HSV2-UL30-6038 - GGUCAAACUCUCCAGCUACAAG 22 14392

HSV2-UL30-6039 - AGGUCAAACUCUCCAGCUACAAG 23 14393

HSV2-UL30-6040 - A AG G U C A A AC UCUCCAGCU AC A AG 24 14394

HSV2-UL30-6041 - AAGCGCAGCAAGAUCAAG 18 14395

HSV2-UL30-6042 - GAAGCGCAGCAAGAUCAAG 19 14396

HSV2-UL30-1871 - AG A AG CG C AG C A AG A U C A AG 20 4820

HSV2-UL30-6043 - CAG AAGCGCAGCAAGAUCAAG 21 14397

HSV2-UL30-6044 - U C AG A AG CG C AG C A AG A U C A AG 22 14398

HSV2-UL30-6045 - U UCAG AAGCGCAGCAAGAUCAAG 23 14399

HSV2-UL30-6046 - U U U C AG A AG CG C AG C A AG A U C A AG 24 14400

HSV2-UL30-6047 - CCGUGCCUCGGGGGGAAG 18 14401

HSV2-UL30-6048 - GCCGUGCCUCGGGGGGAAG 19 14402

HSV2-UL30-581 - GGCCGUGCCUCGGGGGGAAG 20 3451

HSV2-UL30-6049 - CGGCCGUGCCUCGGGGGGAAG 21 14403

HSV2-UL30-6050 - CCGGCCGUGCCUCGGGGGGAAG 22 14404

HSV2-UL30-6051 - CCCGGCCGUGCCUCGGGGGGAAG 23 14405

HSV2-UL30-6052 - GCCCGGCCGUGCCUCGGGGGGAAG 24 14406

HSV2-UL30-6053 - CUGCGCCUUGCGGGCCAG 18 14407

HSV2-UL30-6054 - CCUGCGCCUUGCGGGCCAG 19 14408

HSV2-UL30-1894 - UCCUGCGCCUUGCGGGCCAG 20 4840

HSV2-UL30-6055 - CUCCUGCGCCUUGCGGGCCAG 21 14409

HSV2-UL30-6056 - CCUCCUGCGCCUUGCGGGCCAG 22 14410

HSV2-UL30-6057 - GCCUCCUGCGCCUUGCGGGCCAG 23 14411

HSV2-UL30-6058 - UGCCUCCUGCGCCUUGCGGGCCAG 24 14412 HSV2-UL30-6059 - GCGAUCAGGAAGUACGAG 18 14413

HSV2-UL30-6060 - CGCGAUCAGGAAGUACGAG 19 14414

HSV2-UL30-475 - CCGCGAUCAGGAAGUACGAG 20 3345

HSV2-UL30-6061 - CCCGCGAUCAGGAAGUACGAG 21 14415

HSV2-UL30-6062 - CCCCGCGAUCAGGAAGUACGAG 22 14416

HSV2-UL30-6063 - GCCCCGCGAUCAGGAAGUACGAG 23 14417

HSV2-UL30-6064 - UGCCCCGCGAUCAGGAAGUACGAG 24 14418

HSV2-UL30-6065 - GACUACCUGGAGAUCGAG 18 14419

HSV2-UL30-6066 - GGACUACCUGGAGAUCGAG 19 14420

HSV2-UL30-1958 - GGGACUACCUGGAGAUCGAG 20 4874

HSV2-UL30-6067 - CGGGACUACCUGGAGAUCGAG 21 14421

HSV2-UL30-6068 - CCGGGACUACCUGGAGAUCGAG 22 14422

HSV2-UL30-6069 - ACCGGGACUACCUGGAGAUCGAG 23 14423

HSV2-UL30-6070 - GACCGGGACUACCUGGAGAUCGAG 24 14424

HSV2-UL30-6071 - UCCGCGG ACCACU UCG AG 18 14425

HSV2-UL30-6072 - CUCCGCGG ACCACU UCG AG 19 14426

HSV2-UL30-1806 - UCUCCGCGGACCACUUCGAG 20 4780

HSV2-UL30-6073 - AUCUCCGCGGACCACUUCGAG 21 14427

HSV2-UL30-6074 - CAUCUCCGCGGACCACUUCGAG 22 14428

HSV2-UL30-6075 - GCAUCUCCGCGGACCACUUCGAG 23 14429

HSV2-UL30-6076 - GGCAUCUCCGCGGACCACUUCGAG 24 14430

HSV2-UL30-6077 - CG CA U CACCG ACCCG G AG 18 14431

HSV2-UL30-6078 - GCGCAUCACCGACCCGGAG 19 14432

HSV2-UL30-702 - GGCGCAUCACCGACCCGGAG 20 3572

HSV2-UL30-6079 - CGGCGCAUCACCGACCCGGAG 21 14433

HSV2-UL30-6080 - UCGGCGCAUCACCGACCCGGAG 22 14434

HSV2-UL30-6081 - AUCGGCGCAUCACCGACCCGGAG 23 14435

HSV2-UL30-6082 - CAUCGGCGCAUCACCGACCCGGAG 24 14436

HSV2-UL30-6083 - GCCGUCGCGCACCUGGAG 18 14437

HSV2-UL30-6084 - GGCCGUCGCGCACCUGGAG 19 14438

HSV2-UL30-1952 - AGGCCGUCGCGCACCUGGAG 20 4870

HSV2-UL30-6085 - GAGGCCGUCGCGCACCUGGAG 21 14439

HSV2-UL30-6086 - CGAGGCCGUCGCGCACCUGGAG 22 14440

HSV2-UL30-6087 - CCG AGGCCGUCGCGCACCUGGAG 23 14441

HSV2-UL30-6088 - CCCGAGGCCGUCGCGCACCUGGAG 24 14442

HSV2-UL30-6089 - CCCAGACCCGCGAGGUAG 18 14443

HSV2-UL30-6090 - GCCCAGACCCGCGAGGUAG 19 14444

HSV2-UL30-713 - GGCCCAGACCCGCGAGGUAG 20 3583

HSV2-UL30-6091 - UGGCCCAGACCCGCGAGGUAG 21 14445

HSV2-UL30-6092 - GUGGCCCAGACCCGCGAGGUAG 22 14446

HSV2-UL30-6093 - CG UGGCCCAGACCCGCGAGGUAG 23 14447

HSV2-UL30-6094 - UCGUGGCCCAGACCCGCGAGGUAG 24 14448

HSV2-UL30-6095 - GAGCGGCCGCCGCGUUAG 18 14449

HSV2-UL30-6096 - GGAGCGGCCGCCGCGU UAG 19 14450

HSV2-UL30-1999 - CGGAGCGGCCGCCGCGU UAG 20 4903

HSV2-UL30-6097 - CCGGAGCGGCCGCCGCGUUAG 21 14451 HSV2-UL30-6098 - UCCGGAGCGGCCGCCGCGUUAG 22 14452

HSV2-UL30-6099 - AUCCGGAGCGGCCGCCGCGUUAG 23 14453

HSV2-UL30-6100 - UAUCCGGAGCGGCCGCCGCGUUAG 24 14454

HSV2-UL30-6101 - GUCGAGUU UAACUGCACG 18 14455

HSV2-UL30-6102 - CGUCGAGUUUAACUGCACG 19 14456

HSV2-UL30-1830 - ACGUCGAGUUUAACUGCACG 20 4794

HSV2-UL30-6103 - GACG UCGAGU UUAACUGCACG 21 14457

HSV2-UL30-6104 - CGACGUCGAGU UUAACUGCACG 22 14458

HSV2-UL30-6105 - GCGACGUCGAGU UUAACUGCACG 23 14459

HSV2-UL30-6106 - AGCGACGUCGAGU UUAACUGCACG 24 14460

HSV2-UL30-6107 - ACGAGGAUAAGGACGACG 18 14461

HSV2-UL30-6108 - GACGAGGAUAAGGACGACG 19 14462

HSV2-UL30-1925 - GGACGAGGAUAAGGACGACG 20 4853

HSV2-UL30-6109 - GGG ACGAGGAUAAGGACGACG 21 14463

HSV2-UL30-6110 - GGGG ACGAGGAUAAGGACGACG 22 14464

HSV2-UL30-6111 - CGGGG ACGAGGAUAAGGACGACG 23 14465

HSV2-UL30-6112 - ACGGGGACGAGGAUAAGGACGACG 24 14466

HSV2-UL30-6113 - GCGGGGGCCGGCGCUACG 18 14467

HSV2-UL30-6114 - GGCGGGGGCCGGCGCUACG 19 14468

HSV2-UL30-2058 - CGGCGGGGGCCGGCGCUACG 20 4942

HSV2-UL30-6115 - CCGGCGGGGGCCGGCGCUACG 21 14469

HSV2-UL30-6116 - GCCGGCGGGGGCCGGCGCUACG 22 14470

HSV2-UL30-6117 - GGCCGGCGGGGGCCGGCGCUACG 23 14471

HSV2-UL30-6118 - GGGCCGGCGGGGGCCGGCGCUACG 24 14472

HSV2-UL30-6119 - CCGCGAUCAGGAAGUACG 18 14473

HSV2-UL30-6120 - CCCGCGAUCAGGAAGUACG 19 14474

HSV2-UL30-473 - CCCCGCGAUCAGGAAGUACG 20 3343

HSV2-UL30-6121 - GCCCCGCGAUCAGGAAGUACG 21 14475

HSV2-UL30-6122 - UGCCCCGCGAUCAGGAAGUACG 22 14476

HSV2-UL30-6123 - UUGCCCCGCGAUCAGGAAGUACG 23 14477

HSV2-UL30-6124 - UUUGCCCCGCGAUCAGGAAGUACG 24 14478

HSV2-UL30-6125 - AGGACU UUGUCCUCACCG 18 14479

HSV2-UL30-6126 - CAGGACUU UGUCCUCACCG 19 14480

HSV2-UL30-2014 - CCAGGACUUUGUCCUCACCG 20 4913

HSV2-UL30-6127 - UCCAGGACUU UGUCCUCACCG 21 14481

HSV2-UL30-6128 - AUCCAGGACUUUGUCCUCACCG 22 14482

HSV2-UL30-6129 - CAUCCAGGACUU UGUCCUCACCG 23 14483

HSV2-UL30-6130 - ACAUCCAGGACUUUGUCCUCACCG 24 14484

HSV2-UL30-6131 - CGGCGCAUCACCGACCCG 18 14485

HSV2-UL30-6132 - UCGGCGCAUCACCGACCCG 19 14486

HSV2-UL30-2010 - AUCGGCGCAUCACCGACCCG 20 4910

HSV2-UL30-6133 - CAUCGGCGCAUCACCGACCCG 21 14487

HSV2-UL30-6134 - CCAUCGGCGCAUCACCGACCCG 22 14488

HSV2-UL30-6135 - CCC AU CG G CG CA U CACCG ACCCG 23 14489

HSV2-UL30-6136 - GCCCAUCGGCGCAUCACCGACCCG 24 14490

HSV2-UL30-6137 - UCCCCCAGAGCACCCCCG 18 14491 HSV2-UL30-6138 - AUCCCCCAGAGCACCCCCG 19 14492

HSV2-UL30-641 - GAUCCCCCAGAGCACCCCCG 20 3511

HSV2-UL30-6139 - GGAUCCCCCAGAGCACCCCCG 21 14493

HSV2-UL30-6140 - CGGAUCCCCCAGAGCACCCCCG 22 14494

HSV2-UL30-6141 - GCGGAUCCCCCAGAGCACCCCCG 23 14495

HSV2-UL30-6142 - CGCGGAUCCCCCAGAGCACCCCCG 24 14496

HSV2-UL30-6143 - UCUAGCAUGAGCCCCCCG 18 14497

HSV2-UL30-6144 - CUCUAGCAUGAGCCCCCCG 19 14498

HSV2-UL30-6145 - ACUCUAGCAUGAGCCCCCCG 20 14499

HSV2-UL30-6146 - UACUCUAGCAUGAGCCCCCCG 21 14500

HSV2-UL30-6147 - AUACUCUAGCAUGAGCCCCCCG 22 14501

HSV2-UL30-6148 - GAUACUCUAGCAUGAGCCCCCCG 23 14502

HSV2-UL30-6149 - UGAUACUCUAGCAUGAGCCCCCCG 24 14503

HSV2-UL30-6150 - CGUUAGCCGAGCGCCCCG 18 14504

HSV2-UL30-6151 - GCGUUAGCCGAGCGCCCCG 19 14505

HSV2-UL30-2000 - CG CG U U AG CCG AG CG CCCCG 20 4904

HSV2-UL30-6152 - CCGCGUUAGCCGAGCGCCCCG 21 14506

HSV2-UL30-6153 - GCCGCGUUAGCCGAGCGCCCCG 22 14507

HSV2-UL30-6154 - CGCCGCGUUAGCCGAGCGCCCCG 23 14508

HSV2-UL30-6155 - CCGCCGCGUUAGCCGAGCGCCCCG 24 14509

HSV2-UL30-6156 - UGGACGCCAUCACGCCCG 18 14510

HSV2-UL30-6157 - AUGGACGCCAUCACGCCCG 19 14511

HSV2-UL30-1791 - UAUGGACGCCAUCACGCCCG 20 4769

HSV2-UL30-6158 - UUAUGGACGCCAUCACGCCCG 21 14512

HSV2-UL30-6159 - UUUAUGGACGCCAUCACGCCCG 22 14513

HSV2-UL30-6160 - AUUUAUGGACGCCAUCACGCCCG 23 14514

HSV2-UL30-6161 - GAUUUAUGGACGCCAUCACGCCCG 24 14515

HSV2-UL30-6162 - UGGCGCGACCCCUGCCCG 18 14516

HSV2-UL30-6163 - CUGGCGCGACCCCUGCCCG 19 14517

HSV2-UL30-694 - GCUGGCGCGACCCCUGCCCG 20 3564

HSV2-UL30-6164 - GGCUGGCGCGACCCCUGCCCG 21 14518

HSV2-UL30-6165 - UGGCUGGCGCGACCCCUGCCCG 22 14519

HSV2-UL30-6166 - GUGGCUGGCGCGACCCCUGCCCG 23 14520

HSV2-UL30-6167 - AGUGGCUGGCGCGACCCCUGCCCG 24 14521

HSV2-UL30-6168 - AUAUCGAAUGCAAGGCCG 18 14522

HSV2-UL30-6169 - GAUAUCGAAUGCAAGGCCG 19 14523

HSV2-UL30-502 - CG AUAUCGAAUGCAAGGCCG 20 3372

HSV2-UL30-6170 - UCG AUAUCGAAUGCAAGGCCG 21 14524

HSV2-UL30-6171 - UUCG AUAUCGAAUGCAAGGCCG 22 14525

HSV2-UL30-6172 - CU UCG AUAUCGAAUGCAAGGCCG 23 14526

HSV2-UL30-6173 - GCUUCGAUAUCGAAUGCAAGGCCG 24 14527

HSV2-UL30-6174 - CCG CC U C A AG CCCGGCCG 18 14528

HSV2-UL30-6175 - ACCGCCUCAAGCCCGGCCG 19 14529

HSV2-UL30-486 - UACCGCCUCAAGCCCGGCCG 20 3356

HSV2-UL30-6176 - GU ACCGCCUCAAGCCCGGCCG 21 14530

HSV2-UL30-6177 - GG UACCGCCUCAAGCCCGGCCG 22 14531 HSV2-UL30-6178 - UGGUACCGCCUCAAGCCCGGCCG 23 14532

HSV2-UL30-6179 - CUGGUACCGCCUCAAGCCCGGCCG 24 14533

HSV2-UL30-6180 - GGGGAAGGGGAGCGGCCG 18 14534

HSV2-UL30-6181 - GGGGGAAGGGGAGCGGCCG 19 14535

HSV2-UL30-585 - GGGGGGAAGGGGAGCGGCCG 20 3455

HSV2-UL30-6182 - CGGGGGGAAGGGGAGCGGCCG 21 14536

HSV2-UL30-6183 - UCGGGGGGAAGGGGAGCGGCCG 22 14537

HSV2-UL30-6184 - CUCGGGGGGAAGGGGAGCGGCCG 23 14538

HSV2-UL30-6185 - CCUCGGGGGGAAGGGGAGCGGCCG 24 14539

HSV2-UL30-6186 - GCCGCGGGGUUCGGGCCG 18 14540

HSV2-UL30-6187 - GGCCGCGGGGUUCGGGCCG 19 14541

HSV2-UL30-2055 - GGGCCGCGGGGU UCGGGCCG 20 4941

HSV2-UL30-6188 - AGGGCCGCGGGGUUCGGGCCG 21 14542

HSV2-UL30-6189 - CAGGGCCGCGGGGUUCGGGCCG 22 14543

HSV2-UL30-6190 - UCAGGGCCGCGGGGUUCGGGCCG 23 14544

HSV2-UL30-6191 - CUCAGGGCCGCGGGGUUCGGGCCG 24 14545

HSV2-UL30-6192 - CGGCGGACAACCUGGCCG 18 14546

HSV2-UL30-6193 - ACGGCGGACAACCUGGCCG 19 14547

HSV2-UL30-1831 - CACGGCGGACAACCUGGCCG 20 4795

HSV2-UL30-6194 - GCACGGCGGACAACCUGGCCG 21 14548

HSV2-UL30-6195 - UGCACGGCGGACAACCUGGCCG 22 14549

HSV2-UL30-6196 - CUGCACGGCGGACAACCUGGCCG 23 14550

HSV2-UL30-6197 - ACUGCACGGCGGACAACCUGGCCG 24 14551

HSV2-UL30-6198 - AGGACGGGGACGAGCGCG 18 14552

HSV2-UL30-6199 - GAGGACGGGGACGAGCGCG 19 14553

HSV2-UL30-602 - CG AGGACGGGGACGAGCGCG 20 3472

HSV2-UL30-6200 - ACG AGGACGGGGACGAGCGCG 21 14554

HSV2-UL30-6201 - GACG AGGACGGGGACGAGCGCG 22 14555

HSV2-UL30-6202 - GG ACG AGGACGGGGACGAGCGCG 23 14556

HSV2-UL30-6203 - GGGACGAGGACGGGGACGAGCGCG 24 14557

HSV2-UL30-6204 - CGCGUACGUGCACGCGCG 18 14558

HSV2-UL30-6205 - GCGCGUACGUGCACGCGCG 19 14559

HSV2-UL30-1976 - CGCGCGUACGUGCACGCGCG 20 4887

HSV2-UL30-6206 - G CG CG CG U ACG U G CACG CG CG 21 14560

HSV2-UL30-6207 - CGCGCGCGUACGUGCACGCGCG 22 14561

HSV2-UL30-6208 - ACGCGCGCGUACGUGCACGCGCG 23 14562

HSV2-UL30-6209 - GACGCGCGCGUACGUGCACGCGCG 24 14563

HSV2-UL30-6210 - GGGGCCGGCGCUACGGCG 18 14564

HSV2-UL30-6211 - GGGGGCCGGCGCUACGGCG 19 14565

HSV2-UL30-2060 - CGGGGGCCGGCGCUACGGCG 20 4943

HSV2-UL30-6212 - GCGGGGGCCGGCGCUACGGCG 21 14566

HSV2-UL30-6213 - GGCGGGGGCCGGCGCUACGGCG 22 14567

HSV2-UL30-6214 - CGGCGGGGGCCGGCGCUACGGCG 23 14568

HSV2-UL30-6215 - CCGGCGGGGGCCGGCGCUACGGCG 24 14569

HSV2-UL30-6216 - GCGCCCGGCCGUGCCUCG 18 14570

HSV2-UL30-6217 - AGCGCCCGGCCGUGCCUCG 19 14571 HSV2-UL30-576 - AAGCGCCCGGCCGUGCCUCG 20 3446

HSV2-UL30-6218 - CAAGCGCCCGGCCGUGCCUCG 21 14572

HSV2-UL30-6219 - CCAAGCGCCCGGCCGUGCCUCG 22 14573

HSV2-UL30-6220 - CCCAAGCGCCCGGCCGUGCCUCG 23 14574

HSV2-UL30-6221 - GCCCAAGCGCCCGGCCG UGCCUCG 24 14575

HSV2-UL30-6222 - GCGGCCCUGCGCGAGUCG 18 14576

HSV2-UL30-6223 - GGCGGCCCUGCGCGAGUCG 19 14577

HSV2-UL30-1801 - CGGCGGCCCUGCGCGAGUCG 20 4777

HSV2-UL30-6224 - GCGGCGGCCCUGCGCGAGUCG 21 14578

HSV2-UL30-6225 - GGCGGCGGCCCUGCGCGAGUCG 22 14579

HSV2-UL30-6226 - UGGCGGCGGCCCUGCGCGAGUCG 23 14580

HSV2-UL30-6227 - CUGGCGGCGGCCCUGCGCGAGUCG 24 14581

HSV2-UL30-6228 - ACAAGCUGAACGCCGUCG 18 14582

HSV2-UL30-6229 - UACAAGCUGAACGCCGUCG 19 14583

HSV2-UL30-1876 - CUACAAGCUGAACGCCGUCG 20 4824

HSV2-UL30-6230 - GCU ACAAGCUGAACGCCGUCG 21 14584

HSV2-UL30-6231 - AGCU ACAAGCUGAACGCCGUCG 22 14585

HSV2-UL30-6232 - CAGCU ACAAGCUGAACGCCGUCG 23 14586

HSV2-UL30-6233 - CCAGCU ACAAGCUGAACGCCGUCG 24 14587

HSV2-UL30-6234 - CGGACAACCUGGCCGUCG 18 14588

HSV2-UL30-6235 - GCGGACAACCUGGCCGUCG 19 14589

HSV2-UL30-494 - GGCGGACAACCUGGCCGUCG 20 3364

HSV2-UL30-6236 - CGGCGGACAACCUGGCCGUCG 21 14590

HSV2-UL30-6237 - ACGGCGGACAACCUGGCCGUCG 22 14591

HSV2-UL30-6238 - CACGGCGGACAACCUGGCCGUCG 23 14592

HSV2-UL30-6239 - GCACGGCGGACAACCUGGCCGUCG 24 14593

HSV2-UL30-6240 - AGCUGGCCU UUCCGGUCG 18 14594

HSV2-UL30-6241 - GAGCUGGCCUUUCCGGUCG 19 14595

HSV2-UL30-509 - CGAGCUGGCCUUUCCGG UCG 20 3379

HSV2-UL30-6242 - ACGAGCUGGCCUUUCCGGUCG 21 14596

HSV2-UL30-6243 - GACGAGCUGGCCUUUCCGGUCG 22 14597

HSV2-UL30-6244 - GGACGAGCUGGCCU UUCCGGUCG 23 14598

HSV2-UL30-6245 - AGGACGAGCUGGCCUUUCCGGUCG 24 14599

HSV2-UL30-6246 - ACUUU UACAUGAACAAGG 18 14600

HSV2-UL30-6247 - UACUU UUACAUGAACAAGG 19 14601

HSV2-UL30-451 - GUACUU UUACAUGAACAAGG 20 3321

HSV2-UL30-6248 - AGUACU UUUACAUGAACAAGG 21 14602

HSV2-UL30-6249 - CAG U ACU U U U ACAUG AACAAGG 22 14603

HSV2-UL30-6250 - GCAGUACUUU UACAUGAACAAGG 23 14604

HSV2-UL30-6251 - GGCAGUACU UUUACAUGAACAAGG 24 14605

HSV2-UL30-6252 - UCGAUAUCGAAUGCAAGG 18 14606

HSV2-UL30-6253 - UUCGAUAUCGAAUGCAAGG 19 14607

HSV2-UL30-1836 - CU UCGAUAUCGAAUGCAAGG 20 4798

HSV2-UL30-6254 - GCU UCGAUAUCGAAUGCAAGG 21 14608

HSV2-UL30-6255 - UGCU UCGAUAUCGAAUGCAAGG 22 14609

HSV2-UL30-6256 - GUGCU UCGAUAUCGAAUGCAAGG 23 14610 HSV2-UL30-6257 - UGUGCUUCGAUAUCGAAUGCAAGG 24 14611

HSV2-UL30-6258 - AUAAGGACGACGACGAGG 18 14612

HSV2-UL30-6259 - GAUAAGGACGACGACGAGG 19 14613

HSV2-UL30-1927 - GGAUAAGGACGACGACGAGG 20 4855

HSV2-UL30-6260 - AGGAUAAGGACGACGACGAGG 21 14614

HSV2-UL30-6261 - GAGGAUAAGGACGACGACGAGG 22 14615

HSV2-UL30-6262 - CG AGGAUAAGGACGACGACGAGG 23 14616

HSV2-UL30-6263 - ACGAGGAUAAGGACGACGACGAGG 24 14617

HSV2-UL30-6264 - ACGAGGACGGGGACGAGG 18 14618

HSV2-UL30-6265 - GACGAGGACGGGGACGAGG 19 14619

HSV2-UL30-1932 - CG ACGAGGACGGGGACGAGG 20 4858

HSV2-UL30-6266 - ACG ACGAGGACGGGGACGAGG 21 14620

HSV2-UL30-6267 - GACG ACGAGGACGGGGACGAGG 22 14621

HSV2-UL30-6268 - CG ACG ACGAGGACGGGGACGAGG 23 14622

HSV2-UL30-6269 - ACGACGACGAGGACGGGGACGAGG 24 14623

HSV2-UL30-6270 - UGGCCCAGACCCGCGAGG 18 14624

HSV2-UL30-6271 - GUGGCCCAGACCCGCGAGG 19 14625

HSV2-UL30-2019 - CGUGGCCCAGACCCGCGAGG 20 4918

HSV2-UL30-6272 - UCG UGGCCCAGACCCGCGAGG 21 14626

HSV2-UL30-6273 - AUCG UGGCCCAGACCCGCGAGG 22 14627

HSV2-UL30-6274 - GAUCG UGGCCCAGACCCGCGAGG 23 14628

HSV2-UL30-6275 - UGAUCGUGGCCCAGACCCGCGAGG 24 14629

HSV2-UL30-6276 - ACUACCUGGAGAUCGAGG 18 14630

HSV2-UL30-6277 - GACUACCUGGAGAUCGAGG 19 14631

HSV2-UL30-631 - GG ACUACCUGGAGAUCGAGG 20 3501

HSV2-UL30-6278 - GGG ACUACCUGGAGAUCGAGG 21 14632

HSV2-UL30-6279 - CGGGACUACCUGGAGAUCGAGG 22 14633

HSV2-UL30-6280 - CCGGG ACUACCUGGAGAUCGAGG 23 14634

HSV2-UL30-6281 - ACCGGG ACUACCUGGAGAUCGAGG 24 14635

HSV2-UL30-6282 - CCGCGGACCACU UCGAGG 18 14636

HSV2-UL30-6283 - UCCGCGGACCACUUCGAGG 19 14637

HSV2-UL30-465 - CUCCGCGGACCACUUCGAGG 20 3335

HSV2-UL30-6284 - UCUCCGCGGACCACUUCGAGG 21 14638

HSV2-UL30-6285 - AUCUCCGCGGACCACUUCGAGG 22 14639

HSV2-UL30-6286 - CAUCUCCGCGGACCACUUCGAGG 23 14640

HSV2-UL30-6287 - GCAUCUCCGCGGACCACUUCGAGG 24 14641

HSV2-UL30-6288 - GCAAGGCCGGGGGGGAGG 18 14642

HSV2-UL30-6289 - UGCAAGGCCGGGGGGGAGG 19 14643

HSV2-UL30-1844 - AUGCAAGGCCGGGGGGGAGG 20 4800

HSV2-UL30-6290 - AAUGCAAGGCCGGGGGGGAGG 21 14644

HSV2-UL30-6291 - GAAUGCAAGGCCGGGGGGGAGG 22 14645

HSV2-UL30-6292 - CG AAUGCAAGGCCGGGGGGGAGG 23 14646

HSV2-UL30-6293 - UCGAAUGCAAGGCCGGGGGGGAGG 24 14647

HSV2-UL30-6294 - GUACGGGCGCAUGAACGG 18 14648

HSV2-UL30-6295 - GGUACGGGCGCAUGAACGG 19 14649

HSV2-UL30-1865 - GGGUACGGGCGCAUGAACGG 20 4816 HSV2-UL30-6296 - CGGGUACGGGCGCAUGAACGG 21 14650

HSV2-UL30-6297 - ACGGG U ACGGGCGCAUG AACGG 22 14651

HSV2-UL30-6298 - GACGGGUACGGGCGCAUGAACGG 23 14652

HSV2-UL30-6299 - CGACGGGUACGGGCGCAUGAACGG 24 14653

HSV2-UL30-6300 - CGGGGGCCGGCGCUACGG 18 14654

HSV2-UL30-6301 - GCGGGGGCCGGCGCUACGG 19 14655

HSV2-UL30-763 - GGCGGGGGCCGGCGCUACGG 20 3633

HSV2-UL30-6302 - CGGCGGGGGCCGGCGCUACGG 21 14656

HSV2-UL30-6303 - CCGGCGGGGGCCGGCGCUACGG 22 14657

HSV2-UL30-6304 - GCCGGCGGGGGCCGGCGCUACGG 23 14658

HSV2-UL30-6305 - GGCCGGCGGGGGCCGGCGCUACGG 24 14659

HSV2-UL30-6306 - UAUCGAAUGCAAGGCCGG 18 14660

HSV2-UL30-6307 - AUAUCGAAUGCAAGGCCGG 19 14661

HSV2-UL30-503 - GAUAUCGAAUGCAAGGCCGG 20 3373

HSV2-UL30-6308 - CGAUAUCGAAUGCAAGGCCGG 21 14662

HSV2-UL30-6309 - UCG AUAUCGAAUGCAAGGCCGG 22 14663

HSV2-UL30-6310 - UUCG AUAUCGAAUGCAAGGCCGG 23 14664

HSV2-UL30-6311 - CU UCG AUAUCGAAUGCAAGGCCGG 24 14665

HSV2-UL30-6312 - CCGCGGGG UUCGGGCCGG 18 14666

HSV2-UL30-6313 - GCCGCGGGGUUCGGGCCGG 19 14667

HSV2-UL30-757 - GGCCGCGGGGUUCGGGCCGG 20 3627

HSV2-UL30-6314 - GGGCCGCGGGGUUCGGGCCGG 21 14668

HSV2-UL30-6315 - AGGGCCGCGGGGU UCGGGCCGG 22 14669

HSV2-UL30-6316 - CAGGGCCGCGGGGUUCGGGCCGG 23 14670

HSV2-UL30-6317 - UCAGGGCCGCGGGGUUCGGGCCGG 24 14671

HSV2-UL30-6318 - CGGGGGGAAGGGGAGCGG 18 14672

HSV2-UL30-6319 - UCGGGGGGAAGGGGAGCGG 19 14673

HSV2-UL30-1912 - CUCGGGGGGAAGGGGAGCGG 20 4847

HSV2-UL30-6320 - CCUCGGGGGGAAGGGGAGCGG 21 14674

HSV2-UL30-6321 - GCCUCGGGGGGAAGGGGAGCGG 22 14675

HSV2-UL30-6322 - UGCCUCGGGGGGAAGGGGAGCGG 23 14676

HSV2-UL30-6323 - GUGCCUCGGGGGGAAGGGGAGCGG 24

14677

HSV2-UL30-6324 - GGGCCGGCGCUACGGCGG 18 14678

HSV2-UL30-6325 - GGGGCCGGCGCUACGGCGG 19 14679

HSV2-UL30-764 - GGGGGCCGGCGCUACGGCGG 20 3634

HSV2-UL30-6326 - CGGGGGCCGGCGCUACGGCGG 21 14680

HSV2-UL30-6327 - GCGGGGGCCGGCGCUACGGCGG 22 14681

HSV2-UL30-6328 - GGCGGGGGCCGGCGCUACGGCGG 23 14682

HSV2-UL30-6329 - CGGCGGGGGCCGGCGCUACGGCGG 24 14683

HSV2-UL30-6330 - CGCCCGGCCGUGCCUCGG 18 14684

HSV2-UL30-6331 - GCGCCCGGCCGUGCCUCGG 19 14685

HSV2-UL30-577 - AGCGCCCGGCCGUGCCUCGG 20 3447

HSV2-UL30-6332 - AAGCGCCCGGCCGUGCCUCGG 21 14686

HSV2-UL30-6333 - CAAGCGCCCGGCCGUGCCUCGG 22 14687

HSV2-UL30-6334 - CCAAGCGCCCGGCCGUGCCUCGG 23 14688 HSV2-UL30-6335 - CCCAAGCGCCCGGCCGUGCCUCGG 24 14689

HSV2-UL30-6336 - AUCGAAUGCAAGGCCGGG 18 14690

HSV2-UL30-6337 - UAUCGAAUGCAAGGCCGGG 19 14691

HSV2-UL30-504 - AU AUCGAAUGCAAGGCCGGG 20 3374

HSV2-UL30-6338 - GAU AUCGAAUGCAAGGCCGGG 21 14692

HSV2-UL30-6339 - CGAU AUCGAAUGCAAGGCCGGG 22 14693

HSV2-UL30-6340 - UCGAU AUCGAAUGCAAGGCCGGG 23 14694

HSV2-UL30-6341 - UUCGAUAUCGAAUGCAAGGCCGGG 24 14695

HSV2-UL30-6342 - GCCCGGCCGUGCCUCGGG 18 14696

HSV2-UL30-6343 - CGCCCGGCCGUGCCUCGGG 19 14697

HSV2-UL30-578 - GCGCCCGGCCGUGCCUCGGG 20 3448

HSV2-UL30-6344 - AGCGCCCGGCCGUGCCUCGGG 21 14698

HSV2-UL30-6345 - AAGCGCCCGGCCGUGCCUCGGG 22 14699

HSV2-UL30-6346 - CAAGCGCCCGGCCGUGCCUCGGG 23 14700

HSV2-UL30-6347 - CCAAGCGCCCGGCCGUGCCUCGGG 24 14701

HSV2-UL30-6348 - ACGCCGCCGCCCCAGGGG 18 14702

HSV2-UL30-6349 - GACGCCGCCGCCCCAGGGG 19 14703

HSV2-UL30-2026 - AGACGCCGCCGCCCCAGGGG 20 4922

HSV2-UL30-6350 - U AG ACGCCGCCGCCCCAGGGG 21 14704

HSV2-UL30-6351 - CUAGACGCCGCCGCCCCAGGGG 22 14705

HSV2-UL30-6352 - GCU AGACGCCGCCGCCCCAGGGG 23 14706

HSV2-UL30-6353 - AGCU AG ACGCCGCCGCCCCAGGGG 24 14707

HSV2-UL30-6354 - CGGGGGACGGGAACGGGG 18 14708

HSV2-UL30-6355 - CCGGGGGACGGGAACGGGG 19 14709

HSV2-UL30-1922 - GCCGGGGGACGGGAACGGGG 20 4850

HSV2-UL30-6356 - GGCCGGGGGACGGGAACGGGG 21 14710

HSV2-UL30-6357 - CGGCCGGGGGACGGGAACGGGG 22 14711

HSV2-UL30-6358 - GCGGCCGGGGGACGGGAACGGGG 23 14712

HSV2-UL30-6359 - AGCGGCCGGGGGACGGGAACGGGG 24 14713

HSV2-UL30-6360 - ACGACGACGAGGACGGGG 18 14714

HSV2-UL30-6361 - GACGACGACGAGGACGGGG 19 14715

HSV2-UL30-1930 - GGACGACGACGAGGACGGGG 20 4856

HSV2-UL30-6362 - AGG ACGACGACGAGGACGGGG 21 14716

HSV2-UL30-6363 - AAGG ACGACGACGAGGACGGGG 22 14717

HSV2-UL30-6364 - UAAGG ACGACGACGAGGACGGGG 23 14718

HSV2-UL30-6365 - AUAAGGACGACGACGAGGACGGGG 24 14719

HSV2-UL30-6366 - ACGGGGACGAGGACGGGG 18 14720

HSV2-UL30-6367 - GACGGGGACGAGGACGGGG 19 14721

HSV2-UL30-1935 - GG ACGGGGACGAGGACGGGG 20 4859

HSV2-UL30-6368 - AGG ACGGGGACGAGGACGGGG 21 14722

HSV2-UL30-6369 - G AGG ACGGGGACGAGGACGGGG 22 14723

HSV2-UL30-6370 - CG AGG ACGGGGACGAGGACGGGG 23 14724

HSV2-UL30-6371 - ACGAGGACGGGGACGAGGACGGGG 24 14725

HSV2-UL30-6372 - UCGAAUGCAAGGCCGGGG 18 14726

HSV2-UL30-6373 - AUCGAAUGCAAGGCCGGGG 19 14727

HSV2-UL30-505 - UAUCGAAUGCAAGGCCGGGG 20 3375 HSV2-UL30-6374 - AUAUCGAAUGCAAGGCCGGGG 21 14728

HSV2-UL30-6375 - GAUAUCGAAUGCAAGGCCGGGG 22 14729

HSV2-UL30-6376 - CG AUAUCGAAUGCAAGGCCGGGG 23 14730

HSV2-UL30-6377 - UCGAUAUCGAAUGCAAGGCCGGGG 24 14731

HSV2-UL30-6378 - AAGGGGAGCGGCCGGGGG 18 14732

HSV2-UL30-6379 - GAAGGGGAGCGGCCGGGGG 19 14733

HSV2-UL30-1916 - GG AAGGGGAGCGGCCGGGGG 20 4848

HSV2-UL30-6380 - GGGAAGGGGAGCGGCCGGGGG 21 14734

HSV2-UL30-6381 - GGGG AAGGGGAGCGGCCGGGGG 22 14735

HSV2-UL30-6382 - GGGGG AAGGGGAGCGGCCGGGGG 23 14736

HSV2-UL30-6383 - GGGGGGAAGGGGAGCGGCCGGGGG 24

14737

HSV2-UL30-6384 - GAAUGCAAGGCCGGGGGG 18 14738

HSV2-UL30-6385 - CGAAUGCAAGGCCGGGGGG 19 14739

HSV2-UL30-1843 - UCGAAUGCAAGGCCGGGGGG 20 4799

HSV2-UL30-6386 - AUCGAAUGCAAGGCCGGGGGG 21 14740

HSV2-UL30-6387 - UAUCGAAUGCAAGGCCGGGGGG 22 14741

HSV2-UL30-6388 - AU AUCGAAUGCAAGGCCGGGGGG 23 14742

HSV2-UL30-6389 - GAUAUCGAAUGCAAGGCCGGGGGG 24 14743

HSV2-UL30-6390 - CGGCCGUGCCUCGGGGGG 18 14744

HSV2-UL30-6391 - CCGGCCGUGCCUCGGGGGG 19 14745

HSV2-UL30-1908 - CCCGGCCGUGCCUCGGGGGG 20 4846

HSV2-UL30-6392 - GCCCGGCCGUGCCUCGGGGGG 21 14746

HSV2-UL30-6393 - CGCCCGGCCGUGCCUCGGGGGG 22 14747

HSV2-UL30-6394 - GCGCCCGGCCGUGCCUCGGGGGG 23 14748

HSV2-UL30-6395 - AGCGCCCGGCCGUGCCUCGGGGGG 24 14749

HSV2-UL30-6396 - UGCUGGUGUCCGAGCUGG 18 14750

HSV2-UL30-6397 - CUGCUGGUGUCCGAGCUGG 19 14751

HSV2-UL30-729 - GCUGCUGGUGUCCGAGCUGG 20 3599

HSV2-UL30-6398 - AGCUGCUGGUG UCCGAGCUGG 21 14752

HSV2-UL30-6399 - AAGCUGCUGGUGUCCGAGCUGG 22 14753

HSV2-UL30-6400 - CAAGCUGCUGGUGUCCGAGCUGG 23 14754

HSV2-UL30-6401 - GCAAGCUGCUGGUGUCCGAGCUGG 24 14755

HSV2-UL30-6402 - UACGUGCACGCGCGCUGG 18 14756

HSV2-UL30-6403 - GUACGUGCACGCGCGCUGG 19 14757

HSV2-UL30-1977 - CGUACGUGCACGCGCGCUGG 20 4888

HSV2-UL30-6404 - GCG UACGUGCACGCGCGCUGG 21 14758

HSV2-UL30-6405 - CGCGUACGUGCACGCGCGCUGG 22 14759

HSV2-UL30-6406 - GCGCG UACGUGCACGCGCGCUGG 23 14760

HSV2-UL30-6407 - CGCGCGUACGUGCACGCGCGCUGG 24 14761

HSV2-UL30-6408 - UGCAGGACUCGCUGCUGG 18 14762

HSV2-UL30-6409 - GUGCAGGACUCGCUGCUGG 19 14763

HSV2-UL30-1888 - UGUGCAGGACUCGCUGCUGG 20 4835

HSV2-UL30-6410 - GUG UGCAGGACUCGCUGCUGG 21 14764

HSV2-UL30-6411 - UGUGUGCAGGACUCGCUGCUGG 22 14765

HSV2-UL30-6412 - UUGUGUGCAGGACUCGCUGCUGG 23 14766 HSV2-UL30-6413 - AUUGUGUGCAGGACUCGCUGCUGG 24 14767

HSV2-UL30-6414 - ACU UCGAGGCGGAGGUGG 18 14768

HSV2-UL30-6415 - CACUUCGAGGCGGAGGUGG 19 14769

HSV2-UL30-468 - CCACUUCGAGGCGGAGGUGG 20 3338

HSV2-UL30-6416 - ACCACU UCGAGGCGGAGGUGG 21 14770

HSV2-UL30-6417 - GACCACUUCGAGGCGGAGGUGG 22 14771

HSV2-UL30-6418 - GGACCACUUCGAGGCGGAGGUGG 23 14772

HSV2-UL30-6419 - CGGACCACUUCGAGGCGGAGGUGG 24 14773

HSV2-UL30-6420 - GCCAUGGGCGACAAGAUG 18 14774

HSV2-UL30-6421 - GGCCAUGGGCGACAAGAUG 19 14775

HSV2-UL30-1987 - UGGCCAUGGGCGACAAGAUG 20 4894

HSV2-UL30-6422 - GUGGCCAUGGGCGACAAGAUG 21 14776

HSV2-UL30-6423 - GG UGGCCAUGGGCGACAAGAUG 22 14777

HSV2-UL30-6424 - UGGUGGCCAUGGGCGACAAGAUG 23 14778

HSV2-UL30-6425 - CUGGUGGCCAUGGGCGACAAGAUG 24 14779

HSV2-UL30-6426 - AUCAAGGUGAACGGGAUG 18 14780

HSV2-UL30-6427 - GAUCAAGGUGAACGGGAUG 19 14781

HSV2-UL30-1874 - AG AUCAAGGUGAACGGGAUG 20 4822

HSV2-UL30-6428 - AAG AUCAAGGUGAACGGGAUG 21 14782

HSV2-UL30-6429 - CAAG AUCAAGGUGAACGGGAUG 22 14783

HSV2-UL30-6430 - GCAAG AUCAAGGUGAACGGGAUG 23 14784

HSV2-UL30-6431 - AGCAAGAUCAAGGUGAACGGGAUG 24 14785

HSV2-UL30-6432 - UAUUACUUCUCGCACCUG 18 14786

HSV2-UL30-6433 - CUAUUACUUCUCGCACCUG 19 14787

HSV2-UL30-2041 - ACUAUUACUUCUCGCACCUG 20 4931

HSV2-UL30-6434 - GACUAU UACUUCUCGCACCUG 21 14788

HSV2-UL30-6435 - GGACUAUUACUUCUCGCACCUG 22 14789

HSV2-UL30-6436 - CGG ACU AU U ACU UCUCGCACCUG 23 14790

HSV2-UL30-6437 - ACGGACUAUUACUUCUCGCACCUG 24 14791

HSV2-UL30-6438 - CACGUACGCGAGAGCCUG 18 14792

HSV2-UL30-6439 - CCACGUACGCGAGAGCCUG 19 14793

HSV2-UL30-1965 - CCCACGUACGCGAGAGCCUG 20 4878

HSV2-UL30-6440 - GCCCACGUACGCGAGAGCCUG 21 14794

HSV2-UL30-6441 - GGCCCACG U ACGCG AG AGCCUG 22 14795

HSV2-UL30-6442 - AGGCCCACGUACGCGAGAGCCUG 23 14796

HSV2-UL30-6443 - AAGGCCCACGUACGCGAGAGCCUG 24 14797

HSV2-UL30-6444 - UCCGCCGUCGCGCGCCUG 18 14798

HSV2-UL30-6445 - UUCCGCCGUCGCGCGCCUG 19 14799

HSV2-UL30-1891 - UU UCCGCCGUCGCGCGCCUG 20 4837

HSV2-UL30-6446 - CUUUCCGCCGUCGCGCGCCUG 21 14800

HSV2-UL30-6447 - GCUUUCCGCCGUCGCGCGCCUG 22 14801

HSV2-UL30-6448 - AGCUU UCCGCCGUCGCGCGCCUG 23 14802

HSV2-UL30-6449 - GAGCUUUCCGCCGUCGCGCGCCUG 24 14803

HSV2-UL30-6450 - CAGAAGGGCUUCAUCCUG 18 14804

HSV2-UL30-6451 - CCAGAAGGGCUUCAUCCUG 19 14805

HSV2-UL30-1895 - GCCAGAAGGGCUUCAUCCUG 20 4841 HSV2-UL30-6452 - GGCCAGAAGGGCU UCAUCCUG 21 14806

HSV2-UL30-6453 - GGGCCAGAAGGGCU UCAUCCUG 22 14807

HSV2-UL30-6454 - CGGGCCAGAAGGGCU UCAUCCUG 23 14808

HSV2-UL30-6455 - GCGGGCCAGAAGGGCU UCAUCCUG 24 14809

HSV2-UL30-6456 - UUCGUCCUGACCAAGCUG 18 14810

HSV2-UL30-6457 - CU UCGUCCUGACCAAGCUG 19 14811

HSV2-UL30-1860 - CCUUCG UCCUGACCAAGCUG 20 4812

HSV2-UL30-6458 - CCCUUCGUCCUGACCAAGCUG 21 14812

HSV2-UL30-6459 - GCCCUUCGUCCUGACCAAGCUG 22 14813

HSV2-UL30-6460 - GGCCCUUCGUCCUGACCAAGCUG 23 14814

HSV2-UL30-6461 - UGGCCCU UCGUCCUGACCAAGCUG 24 14815

HSV2-UL30-6462 - CUGCUGGUGUCCGAGCUG 18 14816

HSV2-UL30-6463 - GCUGCUGGUGUCCGAGCUG 19 14817

HSV2-UL30-2034 - AGCUGCUGGUGUCCGAGCUG 20 4926

HSV2-UL30-6464 - AAGCUGCUGGUGUCCGAGCUG 21 14818

HSV2-UL30-6465 - CAAGCUGCUGGUGUCCGAGCUG 22 14819

HSV2-UL30-6466 - GCAAGCUGCUGGUGUCCGAGCUG 23 14820

HSV2-UL30-6467 - CGCAAGCUGCUGGUGUCCGAGCUG 24 14821

HSV2-UL30-6468 - GUACAUCGGCGUCAUCUG 18 14822

HSV2-UL30-6469 - AGUACAUCGGCGUCAUCUG 19 14823

HSV2-UL30-678 - AAGUACAUCGGCGUCAUCUG 20 3548

HSV2-UL30-6470 - AAAGUACAUCGGCGUCAUCUG 21 14824

HSV2-UL30-6471 - AAAAGUACAUCGGCGUCAUCUG 22 14825

HSV2-UL30-6472 - A A A A AG UACAUCGGCGUCAUCUG 23 14826

HSV2-UL30-6473 - GAAAAAGUACAUCGGCGUCAUCUG 24 14827

HSV2-UL30-6474 - CGGGGUGUGU UCCGCGUG 18 14828

HSV2-UL30-6475 - CCGGGGUGUGUUCCGCGUG 19 14829

HSV2-UL30-532 - GCCGGGG UGUGUUCCGCGUG 20 3402

HSV2-UL30-6476 - GGCCGGGGUGUGUUCCGCGUG 21 14830

HSV2-UL30-6477 - CGGCCGGGGUGUGUUCCGCGUG 22 14831

HSV2-UL30-6478 - ACGGCCGGGGUGUGUUCCGCGUG 23 14832

HSV2-UL30-6479 - AACGGCCGGGGUGUGU UCCGCGUG 24 14833

HSV2-UL30-6480 - UACCUGGAGAUCGAGGUG 18 14834

HSV2-UL30-6481 - CUACCUGGAGAUCGAGGUG 19 14835

HSV2-UL30-633 - ACUACCUGGAGAUCGAGGUG 20 3503

HSV2-UL30-6482 - GACUACCUGGAGAUCGAGGUG 21 14836

HSV2-UL30-6483 - GG ACUACCUGGAGAUCGAGGUG 22 14837

HSV2-UL30-6484 - GGG ACUACCUGGAGAUCGAGGUG 23 14838

HSV2-UL30-6485 - CGGGACUACCUGGAGAUCGAGGUG 24 14839

HSV2-UL30-6486 - CACUUCGAGGCGGAGGUG 18 14840

HSV2-UL30-6487 - CCACU UCGAGGCGGAGGUG 19 14841

HSV2-UL30-1808 - ACCACUUCGAGGCGGAGGUG 20 4781

HSV2-UL30-6488 - GACCACUUCGAGGCGGAGGUG 21 14842

HSV2-UL30-6489 - GGACCACUUCGAGGCGGAGGUG 22 14843

HSV2-UL30-6490 - CGGACCACU UCGAGGCGGAGGUG 23 14844

HSV2-UL30-6491 - GCGGACCACU UCGAGGCGGAGGUG 24 14845 HSV2-UL30-6492 - AUCGGCGAGUAUUGUGUG 18 14846

HSV2-UL30-6493 - GAUCGGCGAGUAUUG UGUG 19 14847

HSV2-UL30-1887 - UGAUCGGCGAGUAU UGUGUG 20 4834

HSV2-UL30-6494 - GUG AUCGGCGAGUAUUGUGUG 21 14848

HSV2-UL30-6495 - GGUGAUCGGCGAGUAU UGUGUG 22 14849

HSV2-UL30-6496 - GGGUGAUCGGCGAGUAU UGUGUG 23 14850

HSV2-UL30-6497 - GGGGUGAUCGGCGAGUAUUGUGUG 24

14851

HSV2-UL30-6498 - GUCGCCGAGGCCGUCUUG 18 14852

HSV2-UL30-6499 - CGUCGCCGAGGCCGUCU UG 19 14853

HSV2-UL30-1878 - CCGUCGCCGAGGCCGUCUUG 20 4826

HSV2-UL30-6500 - GCCGUCGCCGAGGCCGUCUUG 21 14854

HSV2-UL30-6501 - CGCCGUCGCCGAGGCCGUCUUG 22 14855

HSV2-UL30-6502 - ACGCCGUCGCCGAGGCCGUCUUG 23 14856

HSV2-UL30-6503 - AACGCCGUCGCCGAGGCCGUCUUG 24 14857

HSV2-UL30-6504 - UGGAGU UUGACAGCGAAU 18 14858

HSV2-UL30-6505 - CUGGAGUU UGACAGCGAAU 19 14859

HSV2-UL30-1857 - CCUGGAGUUUGACAGCGAAU 20 4809

HSV2-UL30-6506 - UCCUGGAGU UUGACAGCGAAU 21 14860

HSV2-UL30-6507 - GUCCUGGAGUUUGACAGCGAAU 22 14861

HSV2-UL30-6508 - CG UCCUGGAGU UUGACAGCGAAU 23 14862

HSV2-UL30-6509 - UCGUCCUGGAGU UUGACAGCGAAU 24 14863

HSV2-UL30-6510 - GCGCGACUGGCUGGCCAU 18 14864

HSV2-UL30-6511 - UGCGCGACUGGCUGGCCAU 19 14865

HSV2-UL30-1966 - CUGCGCGACUGGCUGGCCAU 20 4879

HSV2-UL30-6512 - GCUGCGCGACUGGCUGGCCAU 21 14866

HSV2-UL30-6513 - UGCUGCGCGACUGGCUGGCCAU 22 14867

HSV2-UL30-6514 - CUGCUGCGCGACUGGCUGGCCAU 23 14868

HSV2-UL30-6515 - CCUGCUGCGCGACUGGCUGGCCAU 24 14869

HSV2-UL30-6516 - CAACUUU UGCCCCGCGAU 18 14870

HSV2-UL30-6517 - ACAACUU UUGCCCCGCGAU 19 14871

HSV2-UL30-1813 - GACAACUU UUGCCCCGCGAU 20 4785

HSV2-UL30-6518 - CGACAACUUUUGCCCCGCGAU 21 14872

HSV2-UL30-6519 - GCGACAACUUUUGCCCCGCGAU 22 14873

HSV2-UL30-6520 - UGCG ACAACU U U UGCCCCGCG AU 23 14874

HSV2-UL30-6521 - GUGCGACAACU U UUGCCCCGCGAU 24 14875

HSV2-UL30-6522 - AAGGACAAGAAGAAGGAU 18 14876

HSV2-UL30-6523 - GAAGGACAAGAAGAAGGAU 19 14877

HSV2-UL30-1882 - UG AAGGACAAGAAGAAGGAU 20 4830

HSV2-UL30-6524 - UUG AAGGACAAGAAGAAGGAU 21 14878

HSV2-UL30-6525 - CUUGAAGGACAAG A AG A AG G A U 22 14879

HSV2-UL30-6526 - UCU UG AAGGACAAGAAGAAGGAU 23 14880

HSV2-UL30-6527 - GUCUUGAAGGACAAGAAGAAGGAU 24 14881

HSV2-UL30-6528 - GGGAACGGGGACGAGGAU 18 14882

HSV2-UL30-6529 - CGGGAACGGGGACGAGGAU 19 14883

HSV2-UL30-1924 - ACGGGAACGGGGACGAGGAU 20 4852 HSV2-UL30-6530 - GACGGGAACGGGGACGAGGAU 21 14884

HSV2-UL30-6531 - GGACGGGAACGGGGACGAGGAU 22 14885

HSV2-UL30-6532 - GGGACGGGAACGGGGACGAGGAU 23 14886

HSV2-UL30-6533 - GGGGACGGGAACGGGGACGAGGAU 24

14887

HSV2-UL30-6534 - UUUACGACGAUACCGUAU 18 14888

HSV2-UL30-6535 - UUUUACGACGAUACCGUAU 19 14889

HSV2-UL30-1997 - G U U U UACGACGAUACCG U AU 20 4902

HSV2-UL30-6536 - UGU UUUACGACGAUACCGUAU 21 14890

HSV2-UL30-6537 - CUGUUU UACGACGAUACCGUAU 22 14891

HSV2-UL30-6538 - GCUGUU UUACGACGAUACCGUAU 23 14892

HSV2-UL30-6539 - UGCUGUUUUACGACGAUACCGUAU 24 14893

HSV2-UL30-6540 - GCAUCUCCGCGGACCACU 18 14894

HSV2-UL30-6541 - GGCAUCUCCGCGGACCACU 19 14895

HSV2-UL30-1805 - CGGCAUCUCCGCGGACCACU 20 4779

HSV2-UL30-6542 - GCGGCAUCUCCGCGGACCACU 21 14896

HSV2-UL30-6543 - CGCGGCAUCUCCGCGGACCACU 22 14897

HSV2-UL30-6544 - CCGCGGCAUCUCCGCGGACCACU 23 14898

HSV2-UL30-6545 - UCCGCGGCAUCUCCGCGGACCACU 24 14899

HSV2-UL30-6546 - UCCCCGCCUACUACGCCU 18 14900

HSV2-UL30-6547 - AUCCCCGCCUACUACGCCU 19 14901

HSV2-UL30-1883 - CAUCCCCGCCUACUACGCCU 20 4831

HSV2-UL30-6548 - ACAUCCCCGCCUACUACGCCU 21 14902

HSV2-UL30-6549 - GACAUCCCCGCCUACUACGCCU 22 14903

HSV2-UL30-6550 - CGACAUCCCCGCCUACUACGCCU 23 14904

HSV2-UL30-6551 - GCGACAUCCCCGCCUACUACGCCU 24 14905

HSV2-UL30-6552 - AAGCGCCCGGCCGUGCCU 18 14906

HSV2-UL30-6553 - CAAGCGCCCGGCCGUGCCU 19 14907

HSV2-UL30-574 - CCAAGCGCCCGGCCGUGCCU 20 3444

HSV2-UL30-6554 - CCCAAGCGCCCGGCCGUGCCU 21 14908

HSV2-UL30-6555 - G CCCA AG CG CCCG GCCGUGCCU 22 14909

HSV2-UL30-6556 - CGCCCAAGCGCCCGGCCGUGCCU 23 14910

HSV2-UL30-6557 - GCGCCCAAGCGCCCGGCCGUGCCU 24 14911

HSV2-UL30-6558 - CGACGUGGCCGCGCGGCU 18 14912

HSV2-UL30-6559 - ACGACGUGGCCGCGCGGCU 19 14913

HSV2-UL30-2051 - GACGACGUGGCCGCGCGGCU 20 4938

HSV2-UL30-6560 - GG ACGACGUGGCCGCGCGGCU 21 14914

HSV2-UL30-6561 - CGGACGACGUGGCCGCGCGGCU 22 14915

HSV2-UL30-6562 - CCGG ACGACGUGGCCGCGCGGCU 23 14916

HSV2-UL30-6563 - CCCGGACGACGUGGCCGCGCGGCU 24 14917

HSV2-UL30-6564 - UUACUUCUCGCACCUGCU 18 14918

HSV2-UL30-6565 - AUUACUUCUCGCACCUGCU 19 14919

HSV2-UL30-738 - UAUUACUUCUCGCACCUGCU 20 3608

HSV2-UL30-6566 - CUAUUACUUCUCGCACCUGCU 21 14920

HSV2-UL30-6567 - ACUAU UACUUCUCGCACCUGCU 22 14921

HSV2-UL30-6568 - GACUAU UACUUCUCGCACCUGCU 23 14922 HSV2-UL30-6569 - GGACUAU UACUUCUCGCACCUGCU 24 14923

HSV2-UL30-6570 - ACUCCAUGCGCAUCAUCU 18 14924

HSV2-UL30-6571 - UACUCCAUGCGCAUCAUCU 19 14925

HSV2-UL30-1981 - GUACUCCAUGCGCAUCAUCU 20 4890

HSV2-UL30-6572 - CGUACUCCAUGCGCAUCAUCU 21 14926

HSV2-UL30-6573 - CCGUACUCCAUGCGCAUCAUCU 22 14927

HSV2-UL30-6574 - UCCGUACUCCAUGCGCAUCAUCU 23 14928

HSV2-UL30-6575 - GUCCGUACUCCAUGCGCAUCAUCU 24 14929

HSV2-UL30-6576 - AGUACAUCGGCGUCAUCU 18 14930

HSV2-UL30-6577 - AAGUACAUCGGCGUCAUCU 19 14931

HSV2-UL30-1991 - AAAGUACAUCGGCGUCAUCU 20 4898

HSV2-UL30-6578 - AAAAGUACAUCGGCGUCAUCU 21 14932

HSV2-UL30-6579 - A A A A AG UACAUCGGCGUCAUCU 22 14933

HSV2-UL30-6580 - G A A A A AG UACAUCGGCGUCAUCU 23 14934

HSV2-UL30-6581 - AGAAAAAG UACAUCGGCGUCAUCU 24 14935

HSV2-UL30-6582 - GUGCCCCGCGCGAUCUCU 18 14936

HSV2-UL30-6583 - CGUGCCCCGCGCGAUCUCU 19 14937

HSV2-UL30-1799 - CCGUGCCCCGCGCGAUCUCU 20 4775

HSV2-UL30-6584 - GCCGUGCCCCGCGCGAUCUCU 21 14938

HSV2-UL30-6585 - UGCCGUGCCCCGCGCGAUCUCU 22 14939

HSV2-UL30-6586 - GUGCCGUGCCCCGCGCGAUCUCU 23 14940

HSV2-UL30-6587 - AGUGCCGUGCCCCGCGCGAUCUCU 24 14941

HSV2-UL30-6588 - GCCCCGCG AUCAGG AAG U 18 14942

HSV2-UL30-6589 - UGCCCCGCGAUCAGGAAGU 19 14943

HSV2-UL30-1815 - UUGCCCCGCGAUCAGGAAGU 20 4786

HSV2-UL30-6590 - U UUGCCCCGCGAUCAGGAAGU 21 14944

HSV2-UL30-6591 - UUUUGCCCCGCGAUCAGGAAGU 22 14945

HSV2-UL30-6592 - CUUUUGCCCCGCGAUCAGGAAGU 23 14946

HSV2-UL30-6593 - ACUUU UGCCCCGCGAUCAGGAAGU 24 14947

HSV2-UL30-6594 - CCCCGAUCAAGCUCGAGU 18 14948

HSV2-UL30-6595 - CCCCCGAUCAAGCUCGAGU 19 14949

HSV2-UL30-1989 - CCCCCCG AUCAAG CU CG AG U 20 4896

HSV2-UL30-6596 - UCCCCCCGAUCAAGCUCGAGU 21 14950

HSV2-UL30-6597 - CUCCCCCCGAUCAAGCUCGAGU 22 14951

HSV2-UL30-6598 - CCUCCCCCCGAUCAAGCUCGAGU 23 14952

HSV2-UL30-6599 - UCCUCCCCCCGAUCAAGCUCGAGU 24 14953

HSV2-UL30-6600 - CCGGGGUGUGU UCCGCGU 18 14954

HSV2-UL30-6601 - GCCGGGGUG UGUUCCGCGU 19 14955

HSV2-UL30-1867 - GGCCGGGGUG UGU UCCGCGU 20 4817

HSV2-UL30-6602 - CGGCCGGGGUGUGU UCCGCGU 21 14956

HSV2-UL30-6603 - ACGGCCGGGGUGUGUUCCGCGU 22 14957

HSV2-UL30-6604 - AACGGCCGGGGUGUGU UCCGCGU 23 14958

HSV2-UL30-6605 - GAACGGCCGGGGUGUGU UCCGCGU 24 14959

HSV2-UL30-6606 - CCGAGGGACUGCAGGCGU 18 14960

HSV2-UL30-6607 - CCCGAGGGACUGCAGGCGU 19 14961

HSV2-UL30-2006 - GCCCGAGGGACUGCAGGCGU 20 4908 HSV2-UL30-6608 - UGCCCGAGGGACUGCAGGCGU 21 14962

HSV2-UL30-6609 - CUGCCCGAGGGACUGCAGGCGU 22 14963

HSV2-UL30-6610 - CCUGCCCGAGGGACUGCAGGCGU 23 14964

HSV2-UL30-6611 - CCCUGCCCGAGGGACUGCAGGCGU 24 14965

HSV2-UL30-6612 - CGCGCCCCCCGACGGCGU 18 14966

HSV2-UL30-6613 - CCGCGCCCCCCGACGGCGU 19 14967

HSV2-UL30-1826 - ACCGCGCCCCCCGACGGCGU 20 4791

HSV2-UL30-6614 - AACCGCGCCCCCCGACGGCGU 21 14968

HSV2-UL30-6615 - CA ACCG CG CCCCCCG ACG G CG U 22 14969

HSV2-UL30-6616 - CCAACCGCGCCCCCCGACGGCGU 23 14970

HSV2-UL30-6617 - CCCA ACCG CG CCCCCCG ACG G CG U 24 14971

HSV2-UL30-6618 - GUACUACCGCGUCUUCGU 18 14972

HSV2-UL30-6619 - UGUACUACCGCGUCU UCGU 19 14973

HSV2-UL30-1811 - CUGUACUACCGCGUCUUCGU 20 4783

HSV2-UL30-6620 - CCUGUACUACCGCGUCU UCGU 21 14974

HSV2-UL30-6621 - CCCUGUACUACCGCGUCUUCGU 22 14975

HSV2-UL30-6622 - ACCCUGUACUACCGCGUCUUCGU 23 14976

HSV2-UL30-6623 - GACCCUGUACUACCGCGUCUUCGU 24 14977

HSV2-UL30-6624 - CUACCUGGAGAUCGAGGU 18 14978

HSV2-UL30-6625 - ACUACCUGGAGAUCGAGGU 19 14979

HSV2-UL30-632 - GACUACCUGGAGAUCGAGGU 20 3502

HSV2-UL30-6626 - GG ACUACCUGGAGAUCGAGGU 21 14980

HSV2-UL30-6627 - GGG ACUACCUGGAGAUCGAGGU 22 14981

HSV2-UL30-6628 - CGGG ACUACCUGGAGAUCGAGGU 23 14982

HSV2-UL30-6629 - CCGGGACUACCUGGAGAUCGAGGU 24 14983

HSV2-UL30-6630 - AGGUCCCGCUCGACGGGU 18 14984

HSV2-UL30-6631 - AAGGUCCCGCUCGACGGGU 19 14985

HSV2-UL30-1863 - CAAGGUCCCGCUCGACGGGU 20 4814

HSV2-UL30-6632 - ACAAGGUCCCGCUCGACGGGU 21 14986

HSV2-UL30-6633 - UACAAGGUCCCGCUCGACGGGU 22 14987

HSV2-UL30-6634 - CUACAAGGUCCCGCUCGACGGGU 23 14988

HSV2-UL30-6635 - UCUACAAGGUCCCGCUCGACGGGU 24 14989

HSV2-UL30-6636 - GGCUCAGGGCCGCGGGGU 18 14990

HSV2-UL30-6637 - CGGCUCAGGGCCGCGGGGU 19 14991

HSV2-UL30-2054 - GCGGCUCAGGGCCGCGGGGU 20 4940

HSV2-UL30-6638 - CGCGGCUCAGGGCCGCGGGGU 21 14992

HSV2-UL30-6639 - GCGCGGCUCAGGGCCGCGGGGU 22 14993

HSV2-UL30-6640 - CGCGCGGCUCAGGGCCGCGGGGU 23 14994

HSV2-UL30-6641 - CCGCGCGGCUCAGGGCCGCGGGGU 24 14995

HSV2-UL30-6642 - UGACGUUCAAGGCCCUGU 18 14996

HSV2-UL30-6643 - G U G ACG U U CA AG GCCCUGU 19 14997

HSV2-UL30-2044 - CGUGACGU U C A AG G CCC U G U 20 4932

HSV2-UL30-6644 - GCGUGACGUUCAAGGCCCUGU 21 14998

HSV2-UL30-6645 - UGCGUGACGUUCAAGGCCCUGU 22 14999

HSV2-UL30-6646 - CUGCGUGACGU UCAAGGCCCUGU 23 15000

HSV2-UL30-6647 - CCUGCGUGACGU UCAAGGCCCUGU 24 15001 HSV2-UL30-6648 - CCCGCAAGCUGCUGG UGU 18 15002

HSV2-UL30-6649 - CCCCGCAAGCUGCUGGUGU 19 15003

HSV2-UL30-2033 - GCCCCGCAAGCUGCUGGUGU 20 4925

HSV2-UL30-6650 - AGCCCCGCAAGCUGCUGGUGU 21 15004

HSV2-UL30-6651 - AAGCCCCGCAAGCUGCUGGUGU 22 15005

HSV2-UL30-6652 - CAAGCCCCGCAAGCUGCUGGUGU 23 15006

HSV2-UL30-6653 - CCA AG CCCCGCAAGCUGCUGGUG U 24 15007

HSV2-UL30-6654 - GCGCCGACGUGUACUAUU 18 15008

HSV2-UL30-6655 - CGCGCCGACGUGUACUAUU 19 15009

HSV2-UL30-1810 - GCGCGCCGACGUGUACUAUU 20 4782

HSV2-UL30-6656 - AGCGCGCCGACGUGUACUAUU 21 15010

HSV2-UL30-6657 - GAGCGCGCCGACGUGUACUAUU 22 15011

HSV2-UL30-6658 - GGAGCGCGCCGACGUGUACUAU U 23 15012

HSV2-UL30-6659 - UGGAGCGCGCCGACGUGUACUAUU 24 15013

HSV2-UL30-6660 - ACGUGCCUCCUGCGCCU U 18 15014

HSV2-UL30-6661 - CACGUGCCUCCUGCGCCU U 19 15015

HSV2-UL30-1892 - UCACGUGCCUCCUGCGCCUU 20 4838

HSV2-UL30-6662 - UUCACGUGCCUCCUGCGCCUU 21 15016

HSV2-UL30-6663 - CU UCACGUGCCUCCUGCGCCUU 22 15017

HSV2-UL30-6664 - UCUUCACGUGCCUCCUGCGCCU U 23 15018

HSV2-UL30-6665 - GUCUUCACGUGCCUCCUGCGCCU U 24 15019

HSV2-UL30-6666 - CGAGGGACUGCAGGCGUU 18 15020

HSV2-UL30-6667 - CCGAGGGACUGCAGGCGU U 19 15021

HSV2-UL30-697 - CCCGAGGGACUGCAGGCGUU 20 3567

HSV2-UL30-6668 - GCCCGAGGGACUGCAGGCGUU 21 15022

HSV2-UL30-6669 - UGCCCGAGGGACUGCAGGCGUU 22 15023

HSV2-UL30-6670 - CUGCCCGAGGGACUGCAGGCGU U 23 15024

HSV2-UL30-6671 - CCUGCCCGAGGGACUGCAGGCGUU 24 15025

HSV2-UL30-6672 - GCGCCCCCCGACGGCG U U 18 15026

HSV2-UL30-6673 - CGCGCCCCCCGACGGCGUU 19 15027

HSV2-UL30-490 - CCGCGCCCCCCGACGGCG UU 20 3360

HSV2-UL30-6674 - ACCGCGCCCCCCGACGGCGUU 21 15028

HSV2-UL30-6675 - AACCGCGCCCCCCGACGGCGU U 22 15029

HSV2-UL30-6676 - CAACCG CGCCCCCCGACGGCGUU 23 15030

HSV2-UL30-6677 - CCAACCGCGCCCCCCGACGGCGU U 24 15031

HSV2-UL30-6678 - GGACACCCAGGGGCGGU U 18 15032

HSV2-UL30-6679 - CGGACACCCAGGGGCGGU U 19 15033

HSV2-UL30-1898 - CCGGACACCCAGGGGCGGUU 20 4843

HSV2-UL30-6680 - GCCGGACACCCAGGGGCGGUU 21 15034

HSV2-UL30-6681 - UGCCGGACACCCAGGGGCGGUU 22 15035

HSV2-UL30-6682 - CUGCCGGACACCCAGGGGCGGUU 23 15036

HSV2-UL30-6683 - CCUGCCGGACACCCAGGGGCGGUU 24 15037

HSV2-UL30-6684 - GACGUUCAAGGCCCUGUU 18 15038

HSV2-UL30-6685 - UGACGUUCAAGGCCCUGUU 19 15039

HSV2-UL30-743 - GUGACGUUCAAGGCCCUGUU 20 3613

HSV2-UL30-6686 - CGUGACGUUCAAGGCCCUGUU 21 15040 HSV2-UL30-6687 - GCGUGACGUUCAAGGCCCUGUU 22 15041

HSV2-UL30-6688 - UGCGUGACGU UCAAGGCCCUGUU 23 15042

HSV2-UL30-6689 - CUGCGUGACGUUCAAGGCCCUGU U 24 15043

HSV2-UL30-3489 - CAGCUCCACG AGCG AU U U 18 11843

HSV2-UL30-3490 - CCAGCUCCACGAGCGAUUU 19 11844

HSV2-UL30-3491 - CCCAGCUCCACGAGCGAUUU 20 11845

HSV2-UL30-3492 - GCCCAGCUCCACGAGCGAUU U 21 11846

HSV2-UL30-3493 - CGCCCAGCUCCACGAGCGAUUU 22 11847

HSV2-UL30-3494 - CCGCCCAGCUCCACGAGCGAUUU 23 11848

HSV2-UL30-3495 - GCCGCCCAGCUCCACGAGCGAU UU 24 11849

HSV2-UL30-6690 - AUCGGCCAGAGCCACUU U 18 15044

HSV2-UL30-6691 - CAUCGGCCAGAGCCACU UU 19 15045

HSV2-UL30-1870 - ACAUCGGCCAGAGCCACU UU 20 4819

HSV2-UL30-6692 - GACAUCGGCCAGAGCCACUU U 21 15046

HSV2-UL30-6693 - GGACAUCGGCCAGAGCCACU UU 22 15047

HSV2-UL30-6694 - GGGACAUCGGCCAGAGCCACUUU 23 15048

HSV2-UL30-6695 - UGGGACAUCGGCCAGAGCCACUUU 24 15049

HSV2-UL30-6696 - CAGCUGCUGGCCGACUU U 18 15050

HSV2-UL30-6697 - UCAGCUGCUGGCCGACUUU 19 15051

HSV2-UL30-1979 - AUCAGCUGCUGGCCGACUUU 20 4889

HSV2-UL30-6698 - GAUCAGCUGCUGGCCGACU UU 21 15052

HSV2-UL30-6699 - CGAUCAGCUGCUGGCCGACUUU 22 15053

HSV2-UL30-6700 - UCGAUCAGCUGCUGGCCGACUUU 23 15054

HSV2-UL30-6701 - UUCGAUCAGCUGCUGGCCGACUU U 24 15055

HSV2-UL30-6702 - GACACCCAGGGGCGGUU U 18 15056

HSV2-UL30-6703 - GGACACCCAGGGGCGGUU U 19 15057

HSV2-UL30-568 - CGGACACCCAGGGGCGGUUU 20 3438

HSV2-UL30-6704 - CCGGACACCCAGGGGCGGUUU 21 15058

HSV2-UL30-6705 - GCCGGACACCCAGGGGCGGUUU 22 15059

HSV2-UL30-6706 - UGCCGGACACCCAGGGGCGGUU U 23 15060

HSV2-UL30-6707 - CUGCCGGACACCCAGGGGCGGUUU 24 15061

Table 10A provides exemplary targeting domains for knocking out the UL30 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 10A

1st Tier DNA Target Site gRNA Name Targeting Domain

Strand Length Seq ID

HSV2-UL30-442 - GCGCCGCCCAGCUCCAC 17 5482

HSV2-UL30-6708 - GCCCGGAGGGCUUCUGGCCG 20 15062

Table 10B provides exemplary targeting domains for knocking out the UL30 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 10B

Table IOC provides exemplary targeting domains for knocking out the UL30 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table IOC

Table 10D provides exemplary targeting domains for knocking out the UL30 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 10D

HSV2-UL30-6728 + CCUCCGCCAGCUCGGACACC 20 15082

HSV2-U L30-554 - CU UCAAGU UUCUGCCGCACC 20 3424

HSV2-UL30-1290 + CU UCCCCCCG AGGCACGGCC 20 4160

HSV2-UL30-6729 + ACACCACCU UG AUGGCGGCC 20 15083

HSV2-UL30-2393 + GGACCUGCGCGCGGCGGGCC 20 5155

HSV2-UL30-1167 + AAUACACCGUCAGGUGGGCC 20 4037

HSV2-UL30-6730 + GCGGGACCU UGUAGAUCUCC 20 15084

HSV2-UL30-6731 + ACU UUUUCUUGGCGAUGAGC 20 15085

HSV2-UL30-6732 + GCACAGGUAGGCCAGCGCGC 20 15086

HSV2-UL30-6733 - ACCUGCCGGCCUACAAGCUC 20 15087

HSV2-UL30-1894 - UCCUGCGCCUUGCGGGCCAG 20 4840

HSV2-UL30-6734 + CGGUCACGAACUCGGGGCCG 20 15088

HSV2-UL30-6735 + AGACGGCCUCGGCGACGGCG 20 15089

HSV2-UL30-1125 + CAUGCGACGGCGUCUCCCGG 20 3995

HSV2-UL30-6736 + UCUGGGGGAUCCGCGAGCGG 20 15090

HSV2-UL30-2567 + GGGCCGGCGCGU UCCCGCGG 20 5280

HSV2-UL30-1111 + GCUCGGACACCAGCAGCUUG 20 3981

HSV2-UL30-6737 + UCCCGU UCACCUUGAUCUUG 20 15091

Table 11A provides exemplary targeting domains for knocking out the UL48 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 11A

HSV2-U L48-179 + GCACUCCCGGUACAUGU 17 5558

HSV2-UL48-1134 - GAGCUACCGGACCGUGU 17 15093

HSV2-UL48-77 + GUCGAGGAGACGGUU A A AC A 20 5567

HSV2-UL48-33 - GCCU UCGUACUACGAGGCCA 20 5508

HSV2-UL48-43 + GCCAUGGCCUCGUAGUACGA 20 5521

HSV2-UL48-3 - GAUCUGUUUGCGGACGCGGA 20 5484

HSV2-UL48-11 - GCCCCUCCGCUGUACGCCAC 20 5489

HSV2-UL48-24 - GACCAACGCCGACAUGUACC 20 5500

HSV2-UL48-6 - G G G U U U CG CCACCG CCCCCC 20 5596

HSV2-UL48-18 - GACGAUCUGGGCUUCAGCGC 20 5495

HSV2-UL48-45 + GUACGAAGGCAGCUCGUCGC 20 5523

HSV2-U L48-106 + GUGUUCUUGGGACCCCCGGC 20 5590

HSV2-U L48-100 + GCGUACAGCGGAGGGGCGGC 20 5640

HSV2-UL48-48 + GGCAGCUCGUCGCGGGUGGC 20 5526

HSV2-UL48-36 - GUUUU UCCGCGGUGAGCUGC 20 5511

HSV2-UL48-68 + GGUACAUGUCGGCGUUGGUC 20 5557

HSV2-UL48-14 - GCCCAUGCCCGUCCCCCCCG 20 5601

HSV2-UL48-78 + GAGACGGU UAAACAGGGCCG 20 5568

HSV2-UL48-34 - GCCAUGGCGCAGUUUU UCCG 20 5509

HSV2-UL48-80 + GACGGUUAAACAGGGCCGCG 20 5570

HSV2-UL48-58 + GUGGGCGCGAAUGUCGAUCG 20 5539

HSV2-UL48-94 + GGCCUGACUCAGCCGACCGG 20 5580

HSV2-UL48-83 + GGUUAAACAGGGCCGCGGGG 20 5627

HSV2-UL48-1135 + GUCGACCAACAGGUCCAUGG 20 15094

HSV2-UL48-1 - GUUGGUCGACGAUCUGUUUG 20 5594

HSV2-UL48-92 + GGGCGGCGAGGGCAUCAGCU 20 5636

HSV2-UL48-22 - GGAACGAGGACCUGUUCUCU 20 5606

HSV2-UL48-55 + GGGGAACGCCACGUCGCCGU 20 5536

HSV2-UL48-1136 - GGAGAGCUACCGGACCGUGU 20 15095

Table 11B provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 11B

HSV2-U L48-144 - AUCG ACAU UCGCGCCCA 17 5513

HSV2-U L48-147 - UUCGUACUACGAGGCCA 17 5516

HSV2-U L48-157 + AUGGCCUCGUAGUACGA 17 5528

HSV2-U L48-211 + CGGUGGCGUACAGCGGA 17 5586

HSV2-UL48-117 - CUGUUUGCGGACGCGGA 17 5486

HSV2-UL48-190 + CGAGGAGACGGUUAAAC 17 5573

HSV2-U L48-228 + ACAGAUCG UCGACCAAC 17 5593

HSV2-UL48-125 - CCUCCGCUGUACGCCAC 17 5492

HSV2-UL48-139 - CCAGCGACGUGAUCGAC 17 5503

HSV2-U L48-118 - UGUU UGCGGACGCGGAC 17 5653

HSV2-UL48-137 - CCAACGCCGACAUGUAC 17 5665

HSV2-U L48-138 - CAACGCCGACAUGUACC 17 5502

HSV2-U L48-193 + CGGU UAAACAGGGCCGC 17 5576

HSV2-UL48-176 + CCAGUCGAUCACGUCGC 17 5552

HSV2-U L48-159 + CGAAGGCAGCUCGUCGC 17 5530

HSV2-UL48-214 + UACAGCGGAGGGGCGGC 17 5698

HSV2-UL48-162 + AGCUCGUCGCGGGUGGC 17 5533

HSV2-U L48-150 - UUUCCGCGGUGAGCUGC 17 5519

HSV2-UL48-129 - CCGUCUCCUCGACGAUC 17 5660

HSV2-U L48-174 + UCGAUCGGGGAGCGCUC 17 5550

HSV2-UL48-1137 + AAU UUGCCAACACGGUC 17 15096

HSV2-UL48-182 + ACAUGUCGGCGUUGGUC 17 5561

HSV2-U L48-116 - CGAUCUGUU UGCGGACG 17 5485

HSV2-U L48-192 + ACG G U U A A AC AG G GCCG 17 5575

HSV2-UL48-148 - AUGGCGCAGUU UUUCCG 17 5517

HSV2-U L48-131 - CGAUCUGGGCUUCAGCG 17 5496

HSV2-UL48-175 + CGAUCGGGGAGCGCUCG 17 5551

HSV2-UL48-188 + UGAAGCCCAGAUCGUCG 17 5683

HSV2-U L48-158 + ACGAAGGCAGCUCG UCG 17 5529

HSV2-UL48-210 + CCGGUGGCGUACAGCGG 17 5696

HSV2-UL48-196 + UUAAACAGGGCCGCGGG 17 5579

HSV2-U L48-160 + AGGCAGCUCGUCGCGGG 17 5531

HSV2-UL48-143 - CGACGUGAUCGACUGGG 17 5512

HSV2-U L48-161 + CAGCUCGUCGCGGG UGG 17 5532

HSV2-U L48-141 - AGCGACGUGAUCGACUG 17 5667

HSV2-UL48-149 - UUUUCCGCGGUGAGCUG 17 5518

HSV2-UL48-1138 + U CG ACC AACAG GUCCAU 17 15097

HSV2-UL48-170 + UGGGCGCGAAUGUCGAU 17 5546

HSV2-UL48-140 - CAGCG ACG UG AUCG ACU 17 5666

HSV2-U L48-206 + CGGCGAGGGCAUCAGCU 17 5694

HSV2-U L48-177 + CAGUCGAUCACG UCGCU 17 5553

HSV2-U L48-130 - CGUCUCCUCGACGAUCU 17 5661

HSV2-U L48-180 + CCGGUACAUGUCGGCG U 17 5559

HSV2-UL48-126 - CGCUGUACGCCACCGGU 17 5493

HSV2-UL48-1139 + CGAGCAAAAAUUUGCCAACA 20 15098

HSV2-UL48-1140 - CCCGGACCCAACCGCCCCCA 20 15099 HSV2-UL48-30 - CCGAUCGACAUUCGCGCCCA 20 5505

HSV2-UL48-42 + ACCGCGGAAAAACUGCGCCA 20 5520

HSV2-UL48-1141 + AUCG UCGACCAACAGGUCCA 20 15100

HSV2-UL48-71 + CUCGUUCCAGGUAUCUAGCA 20 5562

HSV2-UL48-50 + CUCGUCGCGGGUGGCGGGCA 20 5534

HSV2-UL48-84 + AAACAGGGCCGCGGGGGGGA 20 5628

HSV2-UL48-76 + CGUCGAGGAGACGGUUAAAC 20 5566

HSV2-UL48-114 + CAAACAGAUCGUCGACCAAC 20 5591

HSV2-UL48-93 + CUGGGCCUGACUCAGCCGAC 20 5637

HSV2-UL48-25 - UGCCCAGCGACGUGAUCGAC 20 5501

HSV2-UL48-4 - AUCUGUUUGCGGACGCGGAC 20 5595

HSV2-UL48-85 + AACAGGGCCGCGGGGGGGAC 20 5629

HSV2-UL48-23 - CGACCAACGCCGACAUGUAC 20 5607

HSV2-UL48-107 + CU UGGGACCCCCGGCUGGCC 20 5645

HSV2-UL48-1142 + CCAUGGGGGCGGU UGGGUCC 20 15101

HSV2-UL48-91 + UGGGCGGCGAGGGCAUCAGC 20 5635

HSV2-UL48-79 + AGACGGUUAAACAGGGCCGC 20 5569

HSV2-UL48-73 + UCUAGCAUGGUACACAGCGC 20 5564

HSV2-UL48-62 + CCCCCAG UCG AU CACG U CGC 20 5543

HSV2-UL48-15 - UAACCGUCUCCUCGACGAUC 20 5602

HSV2-UL48-57 + CGUGGGCGCGAAUGUCGAUC 20 5538

HSV2-UL48-60 + AUGUCGAUCGGGGAGCGCUC 20 5541

HSV2-UL48-13 - CGCCACCGGUCGGCUGAGUC 20 5491

HSV2-UL48-1143 + A A A A A U U U G CC A AC ACG G U C 20 15102

HSV2-UL48-1144 + UCCAUGGGGGCGGUUGGGUC 20 15103

HSV2-UL48-95 + AGCCGACCGGUGGCGUACAG 20 5581

HSV2-UL48-98 + ACCGGUGGCGUACAGCGGAG 20 5583

HSV2-UL48-20 - CAUGCUAGAUACCUGGAACG 20 5498

HSV2-UL48-31 - CAUUCGCGCCCACGGCGACG 20 5506

HSV2-UL48-5 - UCUGUUUGCGGACGCGGACG 20 5487

HSV2-UL48-2 - CGACGAUCUGU UUGCGGACG 20 5483

HSV2-UL48-32 - CGAGCUGCCUUCGUACUACG 20 5507

HSV2-UL48-54 + UGGGGAACGCCACGUCGCCG 20 5620

HSV2-UL48-17 - CGACGAUCUGGGCUUCAGCG 20 5494

HSV2-UL48-61 + UGUCGAUCGGGGAGCGCUCG 20 5542

HSV2-UL48-74 + CGCUGAAGCCCAGAUCGUCG 20 5625

HSV2-UL48-96 + CGACCGGUGGCGUACAGCGG 20 5638

HSV2-UL48-81 + ACGGUUAAACAGGGCCGCGG 20 5571

HSV2-UL48-82 + CGG UUAAACAGGGCCGCGGG 20 5572

HSV2-UL48-46 + CGAAGGCAGCUCGUCGCGGG 20 5524

HSV2-UL48-29 - CAGCGACGUGAUCGACUGGG 20 5504

HSV2-UL48-28 - CCAGCGACGUGAUCGACUGG 20 5610

HSV2-UL48-1145 + CG U CG ACC A AC AG G U CC A U G 20 15104

HSV2-UL48-27 - CCCAGCGACGUGAUCGACUG 20 5609

HSV2-UL48-35 - AGUU U UUCCGCGGUGAGCUG 20 5510

HSV2-UL48-1146 + UCGUCGACCAACAGGUCCAU 20 15105 HSV2-UL48-56 + CCGUGGGCGCGAAUGUCGAU 20 5537

HSV2-UL48-59 + AAUGUCGAUCGGGGAGCGCU 20 5540

HSV2-UL48-63 + CCCCAGUCGAUCACGUCGCU 20 5544

HSV2-U L48-101 + CGUACAGCGGAGGGGCGGCU 20 5588

HSV2-UL48-16 - AACCGUCUCCUCGACGAUCU 20 5603

HSV2-UL48-103 + AGGGGCGGCUGGGGUGUUCU 20 5642

HSV2-UL48-66 + CUCCCGGUACAUGUCGGCGU 20 5555

HSV2-UL48-12 - CUCCGCUGUACGCCACCGGU 20 5490

HSV2-UL48-1147 + AACAGGUCCAUGGGGGCGGU 20 15106

HSV2-UL48-67 + CGGUACAUGUCGGCGUUGGU 20 5556

HSV2-UL48-1148 - AACCGCCCCCAUGGACCUGU 20 15107

HSV2-UL48-1149 + ACAGGUCCAUGGGGGCGGUU 20 15108

Table 11C provides exemplary targeting domains for knocking out the UL48 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 11C

HSV2-U L48-151 - GCGGUGAGCUGCGGGCG 17 5669

HSV2-U L48-227 + GCUGGCCUGGGGGGCGG 17 5709

HSV2-U L48-165 + GCGGGUGGCGGGCAGGG 17 5675

HSV2-U L48-213 + GGCGUACAGCGGAGGGG 17 5697

HSV2-UL48-202 + GGGGGGACGGGCAUGGG 17 5690

HSV2-UL48-1152 + GACCAACAGGUCCAUGG 17 15111

HSV2-U L48-223 + GACCCCCGGCUGGCCUG 17 5705

HSV2-UL48-167 + GGGUGGCGGGCAGGGUG 17 5677

HSV2-UL48-201 + GCGGGGGGGACGGGCAU 17 5689

HSV2-U L48-222 + GGACCCCCGGCUGGCCU 17 5704

HSV2-UL48-422 - GUUGGCAAAUU UUUGCU 17 5904

HSV2-U L48-217 + GGCGGCUGGGGUGUUCU 17 5700

HSV2-U L48-169 + G AACGCCACG U CGCCG U 17 5545

HSV2-UL48-218 + GCGGCUGGGGUGUUCUU 17 5701

HSV2-UL48-1153 + GGUCCAUGGGGGCGGU U 17 15112

HSV2-UL48-86 + GGCCGCGGGGGGGACGGGCA 20 5630

HSV2-UL48-90 + GGGACGGGCAUGGGCGGCGA 20 5634

HSV2-UL48-97 + GACCGGUGGCGUACAGCGGA 20 5582

HSV2-UL48-40 - GGGCGCGGGAGGAGAGCUAC 20 5614

HSV2-UL48-49 + GCUCGUCGCGGGUGGCGGGC 20 5616

HSV2-UL48-89 + GGGGACGGGCAUGGGCGGCG 20 5633

HSV2-UL48-51 + GUCGCGGGUGGCGGGCAGGG 20 5617

HSV2-UL48-99 + GGUGGCGUACAGCGGAGGGG 20 5639

HSV2-UL48-1154 + G ACCAAC AG GUCCAUGGGGG 20 15113

HSV2-UL48-88 + GCGGGGGGGACGGGCAUGGG 20 5632

HSV2-U L48-111 + GGACCCCCGGCUGGCCUGGG 20 5649

HSV2-UL48-110 + GGGACCCCCGGCUGGCCUGG 20 5648

HSV2-UL48-102 + GUACAGCGGAGGGGCGGCUG 20 5641

HSV2-UL48-87 + GCCGCGGGGGGGACGGGCAU 20 5631

HSV2-UL48-26 - GCCCAGCGACGUGAUCGACU 20 5608

HSV2-UL48-104 + GGGGCGGCUGGGGUGU UCUU 20 5643

Table 11D provides exemplary targeting domains for knocking out the UL48 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 11D

Strand Site

Length

HSV2-UL48-200 + CGCGGGGGGGACGGGCA 17 5688

HSV2-UL48-189 + CCAGAUCGUCGAGGAGA 17 5684

HSV2-UL48-204 + ACGGGCAUGGGCGGCGA 17 5692

HSV2-UL48-198 + CAGGGCCGCGGGGGGGA 17 5686

HSV2-UL48-183 + UCGGGAACCCAGAGAAC 17 5680

HSV2-UL48-199 + AGGGCCGCGGGGGGGAC 17 5687

HSV2-UL48-154 - CGCGGGAGGAGAGCUAC 17 5672

HSV2-UL48-120 - UUUCGCCACCGCCCCCC 17 5654

HSV2-UL48-122 - CGCCCCCCAGGCCAGCC 17 5656

HSV2-UL48-178 + ACAGAAACUUGCACUCC 17 5679

HSV2-UL48-1155 + UGGGGGCGGUUGGGUCC 17 15114

HSV2-UL48-121 - CCGCCCCCCAGGCCAGC 17 5655

HSV2-UL48-187 + AGCAUGGUACACAGCGC 17 5565

HSV2-UL48-152 - CGGUGAGCUGCGGGCGC 17 5670

HSV2-UL48-220 + UUCUUGGGACCCCCGGC 17 5592

HSV2-UL48-163 + CGUCGCGGGUGGCGGGC 17 5674

HSV2-UL48-135 - AACGAGGACCUGUUCUC 17 5663

HSV2-UL48-1156 - CACCGGUCGGCUGAGUC 17 15115

HSV2-UL48-1157 + AUGGGGGCGGUUGGGUC 17 15116

HSV2-UL48-209 + CGACCGGUGGCGUACAG 17 5585

HSV2-UL48-145 - UCGCGCCCACGGCGACG 17 5514

HSV2-UL48-128 - CAUGCCCGUCCCCCCCG 17 5659

HSV2-UL48-186 + UAGCAUGGUACACAGCG 17 5682

HSV2-UL48-208 + CUGACUCAGCCGACCGG 17 5584

HSV2-UL48-124 - CCCCCCAGGCCAGCCGG 17 5658

HSV2-UL48-153 - UGAGCUGCGGGCGCGGG 17 5671

HSV2-UL48-197 + UAAACAGGGCCGCGGGG 17 5685

HSV2-UL48-226 + CCGGCUGGCCUGGGGGG 17 5708

HSV2-UL48-1158 + CAACAGGUCCAUGGGGG 17 15117

HSV2-UL48-225 + CCCCCGGCUGGCCUGGG 17 5707

HSV2-UL48-224 + ACCCCCGGCUGGCCUGG 17 5706

HSV2-UL48-1159 + CGACCAACAGGUCCAUG 17 15118

HSV2-UL48-216 + CAGCGGAGGGGCGGCUG 17 5699

HSV2-UL48-215 + ACAGCGGAGGGGCGGCU 17 5589

HSV2-UL48-136 - ACGAGGACCUGUUCUCU 17 5664

HSV2-UL48-1160 + AGGUCCAUGGGGGCGGU 17 15119

HSV2-UL48-166 + CGGGUGGCGGGCAGGGU 17 5676

HSV2-UL48-181 + UACAUGUCGGCGUUGGU 17 5560

HSV2-UL48-1161 - CGCCCCCAUGGACCUGU 17 15120

HSV2-UL48-75 + AGCCCAGAUCGUCGAGGAGA 20 5626

HSV2-UL48-69 + UGGUCGGGAACCCAGAGAAC 20 5622

HSV2-UL48-19 - UGUGUACCAUGCUAGAUACC 20 5604

HSV2-UL48-105 + UGGGGUGUUCUUGGGACCCC 20 5644

HSV2-UL48-8 - CACCGCCCCCCAGGCCAGCC 20 5598 HSV2-UL48-64 + UCGACAGAAACUUGCACUCC 20 5621

HSV2-UL48-70 + AGAGAACAGGUCCUCGU UCC 20 5623

HSV2-UL48-7 - CCACCGCCCCCCAGGCCAGC 20 5597

HSV2-UL48-38 - CCGCGGUGAGCUGCGGGCGC 20 5612

HSV2-UL48-21 - UGGAACGAGGACCUGU UCUC 20 5605

HSV2-UL48-41 + CCCGCGCCCGCAGCUCACCG 20 5615

HSV2-UL48-9 - ACCG CCCCCCAG G CCAG CCG 20 5599

HSV2-UL48-72 + AUCUAGCAUGGUACACAGCG 20 5624

HSV2-UL48-37 - UCCGCGG UGAGCUGCGGGCG 20 5611

HSV2-UL48-44 + AGUACGAAGGCAGCUCGUCG 20 5522

HSV2-UL48-10 - CCGCCCCCCAGGCCAGCCGG 20 5600

HSV2-UL48-113 + CCGGCUGGCCUGGGGGGCGG 20 5651

HSV2-UL48-39 - CGGUGAGCUGCGGGCGCGGG 20 5613

HSV2-UL48-112 + CCCCCGGCUGGCCUGGGGGG 20 5650

HSV2-UL48-47 + AGGCAGCUCGUCGCGGG UGG 20 5525

HSV2-UL48-109 + UGGGACCCCCGGCUGGCCUG 20 5647

HSV2-UL48-53 + CGCGGG UGGCGGGCAGGGUG 20 5619

HSV2-UL48-108 + UUGGGACCCCCGGCUGGCCU 20 5646

HSV2-UL48-1132 - CGUGU UGGCAAAUU UUUGCU 20 5710

HSV2-UL48-52 + UCGCGGGUGGCGGGCAGGGU 20 5618

HSV2-UL48-65 + CUUGCACUCCCGGUACAUGU 20 5554

Table HE provides exemplary targeting domains for knocking out the UL48 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table HE

HSV2-U L48-529 + CA AG U CGAAG U CG U CCA 17 6011

HSV2-U L48-587 + CACGGGAACUCCCCGCA 17 6069

HSV2-U L48-459 - ACCCCGCCCGUCCUGCA 17 5941

HSV2-U L48-589 + UCCGUUGAUAAACAUCA 17 6071

HSV2-UL48-516 + CAAAGCCCCAUACGAGA 17 5998

HSV2-UL48-476 - UACGGAUCGACAAUCGA 17 5958

HSV2-U L48-437 - CGGCCCGAUCUGU UCGA 17 5919

HSV2-UL48-568 + CUGGUUGCCCUGCAGGA 17 6050

HSV2-UL48-468 - AGCGUCGCGACCUCGGA 17 5950

HSV2-U L48-525 + UGGGUCAUUCCCGGGGA 17 6007

HSV2-UL48-489 - GACGAACUCCGCCUGGA 17 5971

HSV2-U L48-558 + CGCGUGCUCCCGCAUGA 17 6040

HSV2-U L48-574 + GACGGGCGGGGUCGUGA 17 6056

HSV2-UL48-434 - UGAGCCGCGAGAUCCUA 17 5916

HSV2-U L48-502 - CACGACCCCGUCUCGUA 17 5984

HSV2-UL48-474 - CG G ACC AG A A AC A A U U A 17 5956

HSV2-UL48-601 + GGUAUAGAUGCAGAAAC 17 6083

HSV2-U L48-591 + GAAACAGACACGCCAAC 17 6073

HSV2-UL48-586 + AGUCGCCGGGCCUCCAC 17 6068

HSV2-U L48-517 + AAAGCCCCAUACGAGAC 17 5999

HSV2-UL48-1162 - GGGGUAGGAUGUGCGAC 17 15121

HSV2-UL48-569 + UGGUUGCCCUGCAGGAC 17 6051

HSV2-U L48-432 - GCACCACGAUCGCGGAC 17 5914

HSV2-U L48-154 - CGCGGGAGGAGAGCUAC 17 5672

HSV2-UL48-613 + CGCUGGCGCGCAGGUAC 17 6095

HSV2-U L48-614 + CGCGCAGGUACCGGUAC 17 6096

HSV2-UL48-423 - UUUGCUCGGCCCUGUAC 17 5905

HSV2-UL48-460 - CGUCCUGCAGGGCAACC 17 5942

HSV2-U L48-455 - GCGGCGGCGGAGGAACC 17 5937

HSV2-UL48-438 - CGGCCUCUGCUGCGACC 17 5920

HSV2-UL48-1163 - GGGUAGGAUGUGCGACC 17 15122

HSV2-U L48-456 - CGGCGGCGGAGGAACCC 17 5938

HSV2-UL48-577 + GUCGUGAGGGGCGCCCC 17 6059

HSV2-U L48-501 - GUGGAGUCCCCCUCCCC 17 5983

HSV2-U L48-493 - GACGCCCGCCGACGCCC 17 5975

HSV2-UL48-576 + GGUCGUGAGGGGCGCCC 17 6058

HSV2-U L48-500 - CGUGGAGUCCCCCUCCC 17 5982

HSV2-UL48-535 + GGCGGAGUUCGUCUCCC 17 6017

HSV2-UL48-522 + GGUCGUGGGUCAUUCCC 17 6004

HSV2-U L48-550 + GCGCGGCGCCACCAGCC 17 6032

HSV2-U L48-486 - CAU U ACCG ACG U C AG CC 17 5968

HSV2-U L48-425 - UCGGCAGCUACACCGCC 17 5907

HSV2-UL48-1164 + GGGGGGGGCGCGCCGCC 17 15123

HSV2-UL48-488 - GGGAGACGAACUCCGCC 17 5970

HSV2-U L48-602 + GAAACAGGACGCGCGCC 17 6084

HSV2-U L48-433 - CCGCGAGACCGCGCGCC 17 5915 HSV2-U L48-584 + UAGCUCCCGCAGUCGCC 17 6066

HSV2-U L48-481 - ACGCUCCUGCGGAGGCC 17 5963

HSV2-U L48-447 - GAGUUCCCGUGGAGGCC 17 5929

HSV2-U L48-566 + ACCCAGAGGAGCGGGCC 17 6048

HSV2-UL48-562 + ACGAGUCCAACUUGGCC 17 6044

HSV2-UL48-609 + GGCCCCGCAUGUGUGCC 17 6091

HSV2-U L48-464 - CUUUAUGCUGCUGAUCC 17 5946

HSV2-UL48-477 - GGGCCUGCUCGACCUCC 17 5959

HSV2-UL48-592 + ACUGGCGCCAGCUCUCC 17 6074

HSV2-U L48-533 + CCACCUCCUCGCCGUCC 17 6015

HSV2-UL48-528 + CCA AG UCGAAGUCGUCC 17 6010

HSV2-U L48-553 + GAGCGUCAUCGUCGUCC 17 6035

HSV2-U L48-521 + GGGUCGUGGGUCAUUCC 17 6003

HSV2-UL48-579 + CCGCACU UCGCACCAGC 17 6061

HSV2-U L48-439 - GCUGCGACCUGGAGAGC 17 5921

HSV2-UL48-555 + CGUCCGGGAGGUCGAGC 17 6037

HSV2-UL48-565 + AAAGUACCCAGAGGAGC 17 6047

HSV2-U L48-611 + GUGUAGCUGCCGAACGC 17 6093

HSV2-UL48-560 + CUCCGAGGUCGCGACGC 17 6042

HSV2-U L48-450 - GCACCUGAACCUCCCGC 17 5932

HSV2-U L48-606 + UGCGCAGCAUCUCCCGC 17 6088

HSV2-UL48-472 - AGCACGCGUAUAGCCGC 17 5954

HSV2-U L48-551 + ACCAGCCCGGCCUCCGC 17 6033

HSV2-U L48-485 - AUGUCGU UUCUCUCCGC 17 5967

HSV2-UL48-612 + GCCGAACGCUGGCGCGC 17 6094

HSV2-U L48-583 + UUAGCUCCCGCAGUCGC 17 6065

HSV2-UL48-480 - GACGCUCCUGCGGAGGC 17 5962

HSV2-UL48-532 + UCGUCCAGGGCGUCGGC 17 6014

HSV2-U L48-571 + UGCCCUGCAGGACGGGC 17 6053

HSV2-UL48-482 - UCCUGCGGAGGCCGGGC 17 5964

HSV2-UL48-427 - CCG CC AG G C AC AC A U G C 17 5909

HSV2-U L48-470 - GGGCGAGUCCGUCAUGC 17 5952

HSV2-UL48-495 - CU UCGACUUGGAGAUGC 17 5977

HSV2-U L48-449 - GGAGGCCCGGCGACUGC 17 5931

HSV2-U L48-430 - CCGCAACCGCGACCUGC 17 5912

HSV2-UL48-567 + GGGCCUGGU UGCCCUGC 17 6049

HSV2-U L48-458 - GACCCCGCCCGUCCUGC 17 5940

HSV2-UL48-545 + GGUGGUGGACAGUCUGC 17 6027

HSV2-UL48-443 - CGGAUCGCUCACCGUGC 17 5925

HSV2-U L48-588 + AUCCGUUGAUAAACAUC 17 6070

HSV2-U L48-595 + AGGCCGUCGAACAGAUC 17 6077

HSV2-U L48-463 - ACUUUAUGCUGCUGAUC 17 5945

HSV2-U L48-461 - AACCAGGCCCGCUCCUC 17 5943

HSV2-UL48-552 + GGAGCGUCAUCGUCGUC 17 6034

HSV2-UL48-1137 + A A U U U G CC A AC ACG G U C 17 15096

HSV2-U L48-581 + GCACCAGCGGGAGGU UC 17 6063 HSV2-U L48-556 + CGAUCCGUAAUUGUUUC 17 6038

HSV2-U L48-600 + UCUCGCGGCUCAGAAAG 17 6082

HSV2-U L48-578 + GCCGCACUUCGCACCAG 17 6060

HSV2-U L48-563 + C AG C A U A A AG U ACCC AG 17 6045

HSV2-UL48-593 + UCUCCAGGUCGCAGCAG 17 6075

HSV2-UL48-590 + CCG U U G A U A A AC A U C AG 17 6072

HSV2-U L48-564 + UAAAGUACCCAGAGGAG 17 6046

HSV2-UL48-526 + GGGUCAUUCCCGGGGAG 17 6008

HSV2-UL48-575 + ACGGGCGGGGUCGUGAG 17 6057

HSV2-U L48-518 + AAGCCCCAUACGAGACG 17 6000

HSV2-UL48-499 - GGAGAUGCUGGGGGACG 17 5981

HSV2-U L48-506 - GUAUGGGGCUUUGGACG 17 5988

HSV2-U L48-597 + CAUCUGCUCGGCGUACG 17 6079

HSV2-UL48-457 - GGCGGCGGAGGAACCCG 17 5939

HSV2-U L48-557 + UGUU UCUGGUCCGCCCG 17 6039

HSV2-UL48-548 + GCGGGGGCGU UGUCCCG 17 6030

HSV2-UL48-523 + GUCGUGGGUCAUUCCCG 17 6005

HSV2-U L48-445 - CGUGCGGGGAGU UCCCG 17 5927

HSV2-UL48-471 - GAGCACGCGUAUAGCCG 17 5953

HSV2-U L48-559 + GACGGACUCGCCCUCCG 17 6041

HSV2-U L48-484 - CAUGUCGU UUCUCUCCG 17 5966

HSV2-UL48-603 + GACG CG CG CCAG G CG CG 17 6085

HSV2-U L48-490 - ACUCCGCCUGGACGGCG 17 5972

HSV2-U L48-572 + GCCCUGCAGGACGGGCG 17 6054

HSV2-UL48-428 - CGCCAGGCACACAUGCG 17 5910

HSV2-U L48-549 + G AG AG A A ACG AC A U G CG 17 6031

HSV2-UL48-546 + GUGGUGGACAGUCUGCG 17 6028

HSV2-UL48-444 - GGAUCGCUCACCGUGCG 17 5926

HSV2-U L48-475 - UUACGGAUCGACAAUCG 17 5957

HSV2-UL48-431 - GCUGCGCACCACGAUCG 17 5913

HSV2-UL48-605 + GUACCUGUCCGCGAUCG 17 6087

HSV2-U L48-599 + CGGCCCAUAGGAUCUCG 17 6081

HSV2-UL48-604 + CCAGGCGCGCGGUCUCG 17 6086

HSV2-U L48-607 + GCAUCUCCCGCAGG UCG 17 6089

HSV2-U L48-519 + CAUACGAGACGGGGUCG 17 6001

HSV2-UL48-534 + CCUCCUCGCCGUCCAGG 17 6016

HSV2-U L48-491 - CCGCCUGGACGGCGAGG 17 5973

HSV2-UL48-492 - CCUGGACGGCGAGGAGG 17 5974

HSV2-UL48-527 + GGUCAUUCCCGGGGAGG 17 6009

HSV2-U L48-454 - GCGAAGUGCGGCGGCGG 17 5936

HSV2-U L48-453 - GGUGCGAAGUGCGGCGG 17 5935

HSV2-U L48-542 + GUCGGUAAUGGGGGCGG 17 6024

HSV2-U L48-479 - CGAUGACGCUCCUGCGG 17 5961

HSV2-UL48-547 + UGGUGGACAGUCUGCGG 17 6029

HSV2-U L48-452 - GCUGGUGCGAAGUGCGG 17 5934

HSV2-U L48-467 - CAGCGUCGCGACCUCGG 17 5949 HSV2-U L48-531 + GUCGUCCAGGGCGUCGG 17 6013

HSV2-U L48-570 + UUGCCCUGCAGGACGGG 17 6052

HSV2-UL48-1165 - UAGGAUGUGCGACCGGG 17 15124

HSV2-U L48-554 + CGUCAUCGUCGUCCGGG 17 6036

HSV2-UL48-580 + CACU UCGCACCAGCGGG 17 6062

HSV2-UL48-473 - ACGCGUAUAGCCGCGGG 17 5955

HSV2-U L48-153 - UGAGCUGCGGGCGCGGG 17 5671

HSV2-UL48-524 + GUGGGUCAUUCCCGGGG 17 6006

HSV2-UL48-541 + GACG UCGGUAAUGGGGG 17 6023

HSV2-U L48-513 - GCAU UGACGACU UUGGG 17 5995

HSV2-UL48-540 + GCUGACGUCGGUAAUGG 17 6022

HSV2-U L48-610 + CCCGCAUGUGUGCCUGG 17 6092

HSV2-U L48-483 - UGCGGAGGCCGGGCUGG 17 5965

HSV2-UL48-498 - CGACUUGGAGAUGCUGG 17 5980

HSV2-U L48-446 - GCGGGGAGUUCCCGUGG 17 5928

HSV2-UL48-543 + GGUAAUGGGGGCGGUGG 17 6025

HSV2-UL48-512 - GGCAUUGACGACU UUGG 17 5994

HSV2-U L48-582 + UCAGGUGCUCGCGAAUG 17 6064

HSV2-UL48-539 + GGCUGACGUCGGUAAUG 17 6021

HSV2-U L48-426 - ACCGCCAGGCACACAUG 17 5908

HSV2-U L48-469 - AGGGCGAGUCCGUCAUG 17 5951

HSV2-UL48-436 - ACGCCGAGCAGAUGAUG 17 5918

HSV2-U L48-504 - CGACCCCGUCUCGUAUG 17 5986

HSV2-U L48-448 - UGGAGGCCCGGCGACUG 17 5930

HSV2-UL48-429 - GCCGCAACCGCGACCUG 17 5911

HSV2-U L48-478 - CGACGAUGACGCUCCUG 17 5960

HSV2-UL48-497 - UCGACU UGGAGAUGCUG 17 5979

HSV2-UL48-544 + CGGUGGUGGACAGUCUG 17 6026

HSV2-U L48-451 - CCCGCUGGUGCGAAGUG 17 5933

HSV2-UL48-442 - ACGGAUCGCUCACCGUG 17 5924

HSV2-UL48-573 + GGACGGGCGGGGUCGUG 17 6055

HSV2-U L48-608 + CCCGCAGG UCGCGGUUG 17 6090

HSV2-UL48-511 - GGGCAUUGACGACUUUG 17 5993

HSV2-U L48-538 + AGGCUGACGUCGGUAAU 17 6020

HSV2-U L48-598 + CGGCGUACGCGGCCCAU 17 6080

HSV2-UL48-508 - AUGUU UACCGAUGCCAU 17 5990

HSV2-U L48-515 + GUCAAUGCCCAUGGCAU 17 5997

HSV2-UL48-594 + GAGGCCGUCGAACAGAU 17 6076

HSV2-UL48-435 - GAGCCGCGAGAUCCUAU 17 5917

HSV2-U L48-503 - ACGACCCCGUCUCGUAU 17 5985

HSV2-U L48-561 + GGAGUACGAGUCCAACU 17 6043

HSV2-U L48-494 - CCUGGACGACUUCGACU 17 5976

HSV2-U L48-466 - CUCCAGCGUCGCGACCU 17 5948

HSV2-UL48-487 - AUUACCGACGUCAGCCU 17 5969

HSV2-U L48-496 - UUCGACUUGGAGAUGCU 17 5978

HSV2-U L48-596 + GGGCCGCAUCAUCUGCU 17 6078 HSV2-U L48-422 - GUUGGCAAAUUUUUGCU 17 5904

HSV2-U L48-462 - ACCAGGCCCGCUCCUCU 17 5944

HSV2-U L48-465 - GCUGAUCCGGGCCAAGU 17 5947

HSV2-U L48-440 - GGAGAGCUGGCGCCAGU 17 5922

HSV2-UL48-536 + GUCUCCCAGGCUGACGU 17 6018

HSV2-UL48-530 + GAAGUCGUCCAGGGCGU 17 6012

HSV2-U L48-520 + AUACGAGACGGGGUCGU 17 6002

HSV2-UL48-1166 - UGACGACUUUGGGGGGU 17 15125

HSV2-UL48-1134 - GAGCUACCGGACCGUGU 17 15093

HSV2-U L48-509 - AUGGGCAU UGACGACU U 17 5991

HSV2-UL48-505 - CGUCUCGUAUGGGGCU U 17 5987

HSV2-U L48-424 - ACCUGCGCGCCAGCGUU 17 5906

HSV2-U L48-510 - UGGGCAU UGACGACUU U 17 5992

HSV2-UL48-247 - CAGCCCCUGAUGUUUAUCAA 20 5729

HSV2-U L48-343 + UCCCAGGCUGACGUCGGUAA 20 5825

HSV2-UL48-1139 + CGAGCAAAAAUU UGCCAACA 20 15098

HSV2-UL48-421 + GGCGCGCAGGUACCGGUACA 20 5903

HSV2-U L48-320 + CCCAAAGUCGUCAAUGCCCA 20 5802

HSV2-UL48-313 - ACAGAUGU UUACCGAUGCCA 20 5795

HSV2-U L48-391 + CCGCAGUCGCCGGGCCUCCA 20 5873

HSV2-U L48-335 + CUCCAAGUCGAAGUCGUCCA 20 5817

HSV2-UL48-393 + CUCCACGGGAACUCCCCGCA 20 5875

HSV2-U L48-265 - ACGACCCCGCCCGUCCUGCA 20 5747

HSV2-U L48-395 + CGAUCCGUUGAUAAACAUCA 20 5877

HSV2-UL48-322 + GUCCAAAGCCCCAUACGAGA 20 5804

HSV2-U L48-282 - AAUUACGGAUCGACAAUCGA 20 5764

HSV2-UL48-243 - AUGCGGCCCGAUCUGU UCGA 20 5725

HSV2-UL48-374 + GGCCUGGU UGCCCUGCAGGA 20 5856

HSV2-U L48-274 - UCCAGCGUCGCGACCUCGGA 20 5756

HSV2-UL48-331 + UCGUGGGUCAUUCCCGGGGA 20 5813

HSV2-UL48-295 - GGAGACGAACUCCGCCUGGA 20 5777

HSV2-U L48-364 + AUACGCGUGCUCCCGCAUGA 20 5846

HSV2-UL48-380 + CAGGACGGGCGGGGUCGUGA 20 5862

HSV2-U L48-240 - UUCUGAGCCGCGAGAUCCUA 20 5722

HSV2-U L48-308 - ACCCACG ACCCCG UCU CG U A 20 5790

HSV2-UL48-280 - GGGCGG ACCAG AAACAAU U A 20 5762

HSV2-U L48-407 + AGAGGUAUAGAUGCAGAAAC 20 5889

HSV2-UL48-397 + G CU G AAACAG ACACG CCA AC 20 5879

HSV2-UL48-392 + CG CAG U CG CCG GGCCU CCAC 20 5874

HSV2-U L48-323 + UCCAAAGCCCCAUACGAGAC 20 5805

HSV2-UL48-1167 - UGGGGGGUAGGAUGUGCGAC 20 15126

HSV2-U L48-375 + GCCUGGUUGCCCUGCAGGAC 20 5857

HSV2-U L48-238 - UGCGCACCACGAUCGCGGAC 20 5720

HSV2-UL48-40 - GGGCGCGGGAGGAGAGCUAC 20 5614

HSV2-U L48-419 + GAACGCUGGCGCGCAGG UAC 20 5901

HSV2-U L48-420 + UGGCGCGCAGGUACCGG UAC 20 5902 HSV2-U L48-229 - AUUU UUGCUCGGCCCUGUAC 20 5711

HSV2-U L48-266 - GCCCGUCCUGCAGGGCAACC 20 5748

HSV2-U L48-261 - AGUGCGGCGGCGGAGGAACC 20 5743

HSV2-U L48-244 - CGACGGCCUCUGCUGCGACC 20 5726

HSV2-UL48-1168 - GGGGGGUAGGAUGUGCGACC 20 15127

HSV2-UL48-262 - GUGCGGCGGCGGAGGAACCC 20 5744

HSV2-U L48-383 + GGGGUCGUGAGGGGCGCCCC 20 5865

HSV2-UL48-307 - GACGUGGAGUCCCCCUCCCC 20 5789

HSV2-UL48-299 - UAUGACGCCCGCCGACGCCC 20 5781

HSV2-U L48-382 + CGGGGUCGUGAGGGGCGCCC 20 5864

HSV2-UL48-306 - GGACGUGGAGUCCCCCUCCC 20 5788

HSV2-U L48-341 + CCAGGCGGAGUUCG UCUCCC 20 5823

HSV2-U L48-328 + CGGGGUCGUGGGUCAUUCCC 20 5810

HSV2-UL48-356 + CAUGCGCGGCGCCACCAGCC 20 5838

HSV2-U L48-292 - CCCCAUUACCGACGUCAGCC 20 5774

HSV2-UL48-231 - CGU UCGGCAGCUACACCGCC 20 5713

HSV2-UL48-294 - CCUGGGAGACGAACUCCGCC 20 5776

HSV2-U L48-408 + GCAGAAACAGGACGCGCGCC 20 5890

HSV2-UL48-239 - CUACCGCGAGACCGCGCGCC 20 5721

HSV2-U L48-390 + GUUUAGCUCCCGCAGUCGCC 20 5872

HSV2-U L48-287 - AUGACGCUCCUGCGGAGGCC 20 5769

HSV2-UL48-253 - GGGGAGU UCCCGUGGAGGCC 20 5735

HSV2-U L48-372 + AGUACCCAGAGGAGCGGGCC 20 5854

HSV2-U L48-368 + AG U ACG AG UCCAACU UGGCC 20 5850

HSV2-UL48-415 + UGCGGCCCCGCAUGUGUGCC 20 5897

HSV2-U L48-270 - GUACU UUAUGCUGCUGAUCC 20 5752

HSV2-UL48-283 - CGAGGGCCUGCUCGACCUCC 20 5765

HSV2-UL48-398 + CCAACUGGCGCCAGCUCUCC 20 5880

HSV2-U L48-339 + UAUCCACCUCCUCGCCGUCC 20 5821

HSV2-UL48-334 + U C U CCA AG UCGAAGUCG UCC 20 5816

HSV2-UL48-359 + CAGGAGCGUCAUCGUCG UCC 20 5841

HSV2-U L48-327 + ACGGGGUCGUGGGUCAUUCC 20 5809

HSV2-UL48-385 + CCGCCGCACUUCGCACCAGC 20 5867

HSV2-U L48-245 - UCUGCUGCGACCUGGAGAGC 20 5727

HSV2-U L48-361 + CGUCGUCCGGGAGGUCGAGC 20 5843

HSV2-UL48-371 + C A U A A AG UACCCAGAGGAGC 20 5853

HSV2-U L48-417 + GCGGUGUAGCUGCCGAACGC 20 5899

HSV2-UL48-366 + GCCCUCCGAGG UCGCGACGC 20 5848

HSV2-UL48-256 - CGAGCACCUGAACCUCCCGC 20 5738

HSV2-U L48-412 + UGGUGCGCAGCAUCUCCCGC 20 5894

HSV2-U L48-278 - GGGAGCACGCGUAUAGCCGC 20 5760

HSV2-U L48-357 + GCCACCAGCCCGGCCUCCGC 20 5839

HSV2-U L48-291 - CGCAUGUCGUU UCUCUCCGC 20 5773

HSV2-UL48-418 + GCUGCCGAACGCUGGCGCGC 20 5900

HSV2-U L48-389 + GGU UUAGCUCCCGCAGUCGC 20 5871

HSV2-U L48-286 - GAUGACGCUCCUGCGGAGGC 20 5768 HSV2-U L48-338 + AAGUCGUCCAGGGCGUCGGC 20 5820

HSV2-U L48-377 + GGUUGCCCUGCAGGACGGGC 20 5859

HSV2-U L48-288 - CGCUCCUGCGGAGGCCGGGC 20 5770

HSV2-U L48-233 - ACACCGCCAGGCACACAUGC 20 5715

HSV2-UL48-276 - GGAGGGCGAGUCCGUCAUGC 20 5758

HSV2-UL48-301 - CGACUUCGACUUGGAGAUGC 20 5783

HSV2-U L48-255 - CGUGGAGGCCCGGCGACUGC 20 5737

HSV2-UL48-236 - GGGCCGCAACCGCGACCUGC 20 5718

HSV2-UL48-373 + AGCGGGCCUGGU UGCCCUGC 20 5855

HSV2-U L48-264 - CACGACCCCGCCCGUCCUGC 20 5746

HSV2-UL48-351 + GGCGGUGGUGGACAGUCUGC 20 5833

HSV2-U L48-249 - CAACGGAUCGCUCACCG UGC 20 5731

HSV2-U L48-394 + GCGAUCCGU UGAUAAACAUC 20 5876

HSV2-UL48-401 + CAGAGGCCGUCGAACAGAUC 20 5883

HSV2-U L48-269 - GGUACUU UAUGCUGCUGAUC 20 5751

HSV2-UL48-267 - GGCAACCAGGCCCGCUCCUC 20 5749

HSV2-UL48-358 + GCAGGAGCGUCAUCGUCGUC 20 5840

HSV2-UL48-1143 + A A A A A UUUGCCAACACGGUC 20 15102

HSV2-UL48-387 + UUCGCACCAGCGGGAGGUUC 20 5869

HSV2-U L48-362 + UGUCGAUCCGUAAUUGUUUC 20 5844

HSV2-U L48-406 + GGAUCUCGCGGCUCAGAAAG 20 5888

HSV2-UL48-384 + GCCGCCGCACUUCGCACCAG 20 5866

HSV2-U L48-369 + CAGCAGCAUAAAGUACCCAG 20 5851

HSV2-U L48-399 + AGCUCUCCAGGUCGCAGCAG 20 5881

HSV2-UL48-396 + GAUCCGUUGAUAAACAUCAG 20 5878

HSV2-U L48-370 + GCAUAAAGUACCCAGAGGAG 20 5852

HSV2-UL48-332 + CGUGGGUCAUUCCCGGGGAG 20 5814

HSV2-UL48-381 + AGGACGGGCGGGGUCGUGAG 20 5863

HSV2-U L48-324 + CCAAAGCCCCAUACGAGACG 20 5806

HSV2-UL48-305 - CUUGGAGAUGCUGGGGGACG 20 5787

HSV2-UL48-312 - CUCGUAUGGGGCU UUGGACG 20 5794

HSV2-U L48-403 + CAUCAUCUGCUCGGCGUACG 20 5885

HSV2-UL48-263 - UGCGGCGGCGGAGGAACCCG 20 5745

HSV2-U L48-363 + AAUUGUU UCUGGUCCGCCCG 20 5845

HSV2-U L48-354 + UCUGCGGGGGCGUUG UCCCG 20 5836

HSV2-UL48-329 + GGGGUCGUGGGUCAU UCCCG 20 5811

HSV2-U L48-251 - CACCGUGCGGGGAGUUCCCG 20 5733

HSV2-UL48-277 - CGGGAGCACGCGUAUAGCCG 20 5759

HSV2-UL48-365 + CAUGACGGACUCGCCCUCCG 20 5847

HSV2-U L48-290 - GCGCAUGUCGU UUCUCUCCG 20 5772

HSV2-U L48-409 + CAGGACGCGCGCCAGGCGCG 20 5891

HSV2-U L48-296 - CGAACUCCGCCUGGACGGCG 20 5778

HSV2-U L48-378 + GU UGCCCUGCAGGACGGGCG 20 5860

HSV2-UL48-234 - CACCGCCAGGCACACAUGCG 20 5716

HSV2-U L48-355 + GCGGAGAGAAACGACAUGCG 20 5837

HSV2-U L48-352 + GCGGUGGUGGACAG UCUGCG 20 5834 HSV2-U L48-250 - AACGGAUCGCUCACCGUGCG 20 5732

HSV2-U L48-281 - CAAUUACGGAUCGACAAUCG 20 5763

HSV2-U L48-237 - GAUGCUGCGCACCACGAUCG 20 5719

HSV2-U L48-411 + GUAGUACCUGUCCGCGAUCG 20 5893

HSV2-UL48-405 + ACGCGGCCCAUAGGAUCUCG 20 5887

HSV2-UL48-410 + GCGCCAGGCGCGCGGUCUCG 20 5892

HSV2-U L48-413 + GCAGCAUCUCCCGCAGGUCG 20 5895

HSV2-UL48-325 + CCCCAUACGAGACGGGGUCG 20 5807

HSV2-UL48-340 + CCACCUCCUCGCCGUCCAGG 20 5822

HSV2-U L48-297 - ACUCCGCCUGGACGGCGAGG 20 5779

HSV2-UL48-298 - CCGCCUGGACGGCGAGGAGG 20 5780

HSV2-U L48-333 + GUGGGUCAUUCCCGGGGAGG 20 5815

HSV2-U L48-260 - GGUGCGAAGUGCGGCGGCGG 20 5742

HSV2-UL48-259 - GCUGGUGCGAAGUGCGGCGG 20 5741

HSV2-U L48-348 + GACGUCGGUAAUGGGGGCGG 20 5830

HSV2-UL48-285 - CGACGAUGACGCUCCUGCGG 20 5767

HSV2-UL48-353 + CGGUGGUGGACAGUCUGCGG 20 5835

HSV2-U L48-258 - CCCGCUGGUGCGAAGUGCGG 20 5740

HSV2-UL48-273 - CUCCAGCGUCGCGACCUCGG 20 5755

HSV2-U L48-337 + GAAGUCGUCCAGGGCGUCGG 20 5819

HSV2-U L48-376 + UGGUUGCCCUGCAGGACGGG 20 5858

HSV2-UL48-1169 - GGGUAGGAUGUGCGACCGGG 20 15128

HSV2-U L48-360 + GAGCGUCAUCG UCGUCCGGG 20 5842

HSV2-U L48-386 + CCGCACU UCGCACCAGCGGG 20 5868

HSV2-UL48-279 - AGCACGCGUAUAGCCGCGGG 20 5761

HSV2-U L48-330 + GUCGUGGGUCAU UCCCGGGG 20 5812

HSV2-UL48-347 + GCUGACGUCGGUAAUGGGGG 20 5829

HSV2-UL48-319 - UGGGCAUUGACGACUUUGGG 20 5801

HSV2-U L48-346 + CAGGCUGACGUCGGUAAUGG 20 5828

HSV2-UL48-416 + GGCCCCGCAUG UGUGCCUGG 20 5898

HSV2-UL48-289 - UCCUGCGGAGGCCGGGCUGG 20 5771

HSV2-U L48-304 - CU UCGACUUGGAGAUGCUGG 20 5786

HSV2-UL48-252 - CGUGCGGGGAGU UCCCGUGG 20 5734

HSV2-U L48-349 + GUCGGUAAUGGGGGCGGUGG 20 5831

HSV2-U L48-318 - AUGGGCAUUGACGACUU UGG 20 5800

HSV2-UL48-388 + GGUUCAGGUGCUCGCGAAUG 20 5870

HSV2-U L48-345 + CCAGGCUGACGUCGGUAAUG 20 5827

HSV2-UL48-232 - UACACCGCCAGGCACACAUG 20 5714

HSV2-UL48-275 - CGGAGGGCGAGUCCGUCAUG 20 5757

HSV2-U L48-242 - CGUACGCCGAGCAGAUGAUG 20 5724

HSV2-U L48-310 - CCACGACCCCGUCUCGUAUG 20 5792

HSV2-U L48-254 - CCGUGGAGGCCCGGCGACUG 20 5736

HSV2-U L48-235 - GGGGCCGCAACCGCGACCUG 20 5717

HSV2-UL48-284 - GGACGACGAUGACGCUCCUG 20 5766

HSV2-U L48-303 - ACU UCGACUUGGAGAUGCUG 20 5785

HSV2-U L48-350 + GGGCGGUGGUGGACAGUCUG 20 5832 HSV2-U L48-257 - CCUCCCGCUGGUGCGAAGUG 20 5739

HSV2-U L48-248 - UCAACGGAUCGCUCACCGUG 20 5730

HSV2-U L48-379 + GCAGGACGGGCGGGGUCGUG 20 5861

HSV2-U L48-414 + UCUCCCGCAGGUCGCGGUUG 20 5896

HSV2-UL48-317 - CAUGGGCAUUGACGACUUUG 20 5799

HSV2-UL48-344 + CCCAGGCUGACGUCGGUAAU 20 5826

HSV2-U L48-404 + GCUCGGCGUACGCGGCCCAU 20 5886

HSV2-UL48-314 - CAGAUGUUUACCGAUGCCAU 20 5796

HSV2-UL48-321 + GUCGUCAAUGCCCAUGGCAU 20 5803

HSV2-U L48-400 + GCAGAGGCCGUCGAACAGAU 20 5882

HSV2-UL48-241 - UCUGAGCCGCGAGAUCCUAU 20 5723

HSV2-U L48-309 - CCCACGACCCCGUCUCGUAU 20 5791

HSV2-U L48-367 + GCUGGAGUACGAGUCCAACU 20 5849

HSV2-UL48-300 - CGCCCUGG ACG ACU UCG ACU 20 5782

HSV2-U L48-272 - GUACUCCAGCGUCGCGACCU 20 5754

HSV2-UL48-293 - CCCAUUACCGACGUCAGCCU 20 5775

HSV2-UL48-302 - GACU UCGACU UGGAGAUGCU 20 5784

HSV2-U L48-402 + AUCGGGCCGCAUCAUCUGCU 20 5884

HSV2-UL48-1132 - CGUGUUGGCAAAUUUU UGCU 20 5710

HSV2-U L48-268 - GCAACCAGGCCCGCUCCUCU 20 5750

HSV2-U L48-271 - GCUGCUGAUCCGGGCCAAGU 20 5753

HSV2-UL48-246 - CCUGGAGAGCUGGCGCCAGU 20 5728

HSV2-U L48-342 + UUCGUCUCCCAGGCUGACGU 20 5824

HSV2-U L48-336 + GUCGAAGUCGUCCAGGGCGU 20 5818

HSV2-UL48-326 + CCCAUACGAGACGGGGUCGU 20 5808

HSV2-UL48-1170 - CAU UGACGACUU UGGGGGGU 20 15129

HSV2-UL48-1136 - GGAGAGCUACCGGACCGUGU 20 15095

HSV2-UL48-315 - GCCAUGGGCAU UGACGACUU 20 5797

HSV2-U L48-311 - CCCCGUCUCGUAUGGGGCUU 20 5793

HSV2-UL48-230 - GGUACCUGCGCGCCAGCGUU 20 5712

HSV2-UL48-316 - CCAUGGGCAUUGACGACU UU 20 5798

Table 12A provides exemplary targeting domains for knocking out the UL48 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality, and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 12A

1st Tier Target

DNA

gRNA Name Targeting Domain Site

Strand

Length Seq ID

HSV2-UL48-1171 + GUCCAUGGGGGCGGUUGGGUCC 22 15130

HSV2-UL48-1172 + GGUCCAUGGGGGCGGU UGGGUCC 23 15131

HSV2-UL48-1173 + GUACAGCGGAGGGGCGGC 18 15132

HSV2-UL48-100 + GCGUACAGCGGAGGGGCGGC 20 5640

HSV2-UL48-1174 + GGCGUACAGCGGAGGGGCGGC 21 15133

HSV2-UL48-1175 + GUGGCGUACAGCGGAGGGGCGGC 23 15134

HSV2-UL48-1176 + GGUGGCGUACAGCGGAGGGGCGGC 24 15135

HSV2-UL48-1177 + GAACGCCACGUCGCCGUGGGC 21 15136

HSV2-UL48-1178 + GGAACGCCACGUCGCCGUGGGC 22 15137

HSV2-UL48-1179 + GGGAACGCCACGUCGCCGUGGGC 23 15138

HSV2-UL48-1180 + GGGGAACGCCACGUCGCCGUGGGC 24 15139

HSV2-UL48-1181 + GUACGAAGGCAGCUCGUC 18 15140

HSV2-UL48-1182 + GUAGUACGAAGGCAGCUCGUC 21 15141

HSV2-UL48-1183 + GACCAACAGGUCCAUGGGGGCGG 23 15142

HSV2-UL48-1184 + GCUCGUCGCGGGUGGCGGG 19 15143

HSV2-UL48-1185 + GCAGCUCGUCGCGGGUGGCGGG 22 15144

HSV2-UL48-1186 + GGCAGCUCGUCGCGGGUGGCGGG 23 15145

HSV2-UL48-3 - GAUCUGUUUGCGGACGCGGA 20 5484

HSV2-UL48-1187 - GACGAUCUGUUUGCGGACGCGGA 23 15146

HSV2-UL48-24 - GACCAACGCCGACAUGUACC 20 5500

HSV2-UL48-1188 - GCCACCGCCCCCCAGGCCAGCC 22 15147

HSV2-UL48-1189 - GACGAUCUGGGCUUCAGC 18 15148

HSV2-UL48-1190 - GCUGUACGCCACCGGUCGG 19 15149

HSV2-UL48-1191 - GCCCAGCGACGUGAUCGACUGG 22 15150

Table 12B provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 12B

HSV2-UL48-1142 + CCAUGGGGGCGGU UGGGUCC 20 15101

HSV2-UL48-1194 + UCCAUGGGGGCGGUUGGGUCC 21 15153

HSV2-UL48-1195 + AGGUCCAUGGGGGCGG UUGGGUCC 24

15154

HSV2-UL48-1196 + CGUACAGCGGAGGGGCGGC 19 15155

HSV2-UL48-1197 + UGGCGUACAGCGGAGGGGCGGC 22 15156

HSV2-UL48-1198 + CGCCACGUCGCCGUGGGC 18 15157

HSV2-UL48-1199 + ACGCCACGUCGCCGUGGGC 19 15158

HSV2-UL48-733 + AACGCCACGUCGCCGUGGGC 20 6168

HSV2-UL48-1200 + AGUACGAAGGCAGCUCG UC 19 15159

HSV2-UL48-741 + UAGUACGAAGGCAGCUCGUC 20 6173

HSV2-UL48-1201 + CGU AGUACGAAGGCAGCUCGUC 22 15160

HSV2-UL48-1202 + UCG UAGUACGAAGGCAGCUCGUC 23 15161

HSV2-UL48-1203 + CUCGUAGUACGAAGGCAGCUCGUC 24 15162

HSV2-UL48-1204 + ACAGGUCCAUGGGGGCGG 18 15163

HSV2-UL48-1205 + AACAGGUCCAUGGGGGCGG 19 15164

HSV2-UL48-1206 + CAACAGGUCCAUGGGGGCGG 20 15165

HSV2-UL48-1207 + CCAACAGGUCCAUGGGGGCGG 21 15166

HSV2-UL48-1208 + ACCAACAGGUCCAUGGGGGCGG 22 15167

HSV2-UL48-1209 + CGACCAACAGGUCCAUGGGGGCGG 24 15168

HSV2-UL48-1210 + CUCGUCGCGGGUGGCGGG 18 15169

HSV2-UL48-739 + AGCUCGUCGCGGGUGGCGGG 20 6171

HSV2-UL48-1211 + CAGCUCGUCGCGGGUGGCGGG 21 15170

HSV2-UL48-1212 + AGGCAGCUCGUCGCGGGUGGCGGG 24 15171

HSV2-UL48-1213 - UCUGUU UGCGGACGCGGA 18 15172

HSV2-UL48-1214 - AUCUGUUUGCGGACGCGGA 19 15173

HSV2-UL48-1215 - CGAUCUGU UUGCGGACGCGGA 21 15174

HSV2-UL48-1216 - ACG AUCUGUUUGCGGACGCGGA 22 15175

HSV2-UL48-1217 - CGACGAUCUGU UUGCGGACGCGGA 24 15176

HSV2-UL48-1218 - CCAACG CCG AC AU G U ACC 18 15177

HSV2-UL48-1219 - ACCAACGCCGACAUGUACC 19 15178

HSV2-UL48-1220 - CGACCAACGCCGACAUGUACC 21 15179

HSV2-UL48-1221 - CCGACCAACGCCGACAUGUACC 22 15180

HSV2-UL48-1222 - CCCGACCAACGCCGACAUGUACC 23 15181

HSV2-UL48-1223 - UCCCG ACCAACGCCGACAUGUACC 24 15182

HSV2-UL48-1224 - CCACCGCCCCCCAGGCCAGCC 21 15183

HSV2-UL48-1225 - CG CCACCGCCCCCCAG G CCAG CC 23 15184

HSV2-UL48-1226 - U CG CCACCGCCCCCCAG G CCAG CC 24 15185

HSV2-UL48-1227 - CGACGAUCUGGGCUUCAGC 19 15186

HSV2-UL48-626 - UCGACGAUCUGGGCUUCAGC 20 6105

HSV2-UL48-1228 - CUCGACGAUCUGGGCU UCAGC 21 15187

HSV2-UL48-1229 - CCUCGACGAUCUGGGCUUCAGC 22 15188

HSV2-UL48-1230 - UCCUCGACGAUCUGGGCUUCAGC 23 15189

HSV2-UL48-1231 - CUCCUCGACGAUCUGGGCU UCAGC 24 15190

HSV2-UL48-1232 - CUGUACGCCACCGGUCGG 18 15191

HSV2-UL48-624 - CGCUGUACGCCACCGGUCGG 20 6103 HSV2-UL48-1233 - CCGCUGUACGCCACCGGUCGG 21 15192

HSV2-UL48-1234 - UCCGCUGUACGCCACCGGUCGG 22 15193

HSV2-UL48-1235 - CUCCGCUGUACGCCACCGGUCGG 23 15194

HSV2-UL48-1236 - CCUCCGCUGUACGCCACCGGUCGG 24 15195

HSV2-UL48-1237 - AGCGACGUGAUCGACUGG 18 15196

HSV2-UL48-1238 - CAGCGACGUGAUCGACUGG 19 15197

HSV2-UL48-28 - CCAGCGACGUGAUCGACUGG 20 5610

HSV2-UL48-1239 - CCCAGCGACGUGAUCGACUGG 21 15198

HSV2-UL48-1240 - UGCCCAGCGACGUGAUCGACUGG 23 15199

HSV2-UL48-1241 - CUGCCCAGCGACGUGAUCGACUGG 24 15200

HSV2-UL48-1242 - CCUGGAACGAGGACCUGUUCU 21 15201

HSV2-UL48-1243 - ACCUGGAACGAGGACCUGUUCU 22 15202

HSV2-UL48-1244 - UACCUGGAACGAGGACCUGUUCU 23 15203

HSV2-UL48-1245 - AUACCUGGAACGAGGACCUGU UCU 24 15204

Table 12C provides exemplary targeting domains for knocking out the UL48 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 12C

Table 12D provides exemplary targeting domains for knocking out the UL48 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 12D

HSV2-UL48-1284 + GUCGCCGUGGGCGCGAAUGUCGA 23 15243

HSV2-UL48-1285 + CGUCGCCGUGGGCGCGAAUGUCGA 24 15244

HSV2-UL48-1286 + CGGCGUUGGUCGGGAACC 18 15245

HSV2-UL48-1287 + UCGGCGUUGGUCGGGAACC 19 15246

HSV2-UL48-720 + GUCGGCGU UGGUCGGGAACC 20 6162

HSV2-UL48-1288 + UGUCGGCGUUGGUCGGGAACC 21 15247

HSV2-UL48-1289 + AUG UCGGCGU UGGUCGGGAACC 22 15248

HSV2-UL48-1290 + CAUG UCGGCGUUGGUCGGGAACC 23 15249

HSV2-UL48-1291 + ACAUGUCGGCGU UGGUCGGGAACC 24 15250

HSV2-UL48-1292 + CCGCGCCCGCAGCUCACC 18 15251

HSV2-UL48-1293 + CCCGCGCCCGCAGCUCACC 19 15252

HSV2-UL48-744 + UCCCGCGCCCGCAGCUCACC 20 6175

HSV2-UL48-1294 + CUCCCGCGCCCGCAGCUCACC 21 15253

HSV2-UL48-1295 + CCUCCCGCGCCCGCAGCUCACC 22 15254

HSV2-UL48-1296 + UCCUCCCGCGCCCGCAGCUCACC 23 15255

HSV2-UL48-1297 + CUCCUCCCGCGCCCGCAGCUCACC 24 15256

HSV2-UL48-1298 + GGGGAACGCCACGUCGCC 18 15257

HSV2-UL48-1299 + UGGGGAACGCCACGUCGCC 19 15258

HSV2-UL48-734 + GUGGGGAACGCCACGUCGCC 20 6169

HSV2-UL48-1300 + GG UGGGGAACGCCACGUCGCC 21 15259

HSV2-UL48-1301 + GGG UGGGGAACGCCACGUCGCC 22 15260

HSV2-UL48-1302 + AGGGUGGGGAACGCCACGUCGCC 23 15261

HSV2-UL48-1303 + CAGGG UGGGGAACGCCACGUCGCC 24 15262

HSV2-UL48-1304 + AGACGGU UAAACAGGGCC 18 15263

HSV2-UL48-1305 + GAGACGGUUAAACAGGGCC 19 15264

HSV2-UL48-711 + GGAGACGGU UAAACAGGGCC 20 6155

HSV2-UL48-1306 + AGGAGACGGUUAAACAGGGCC 21 15265

HSV2-UL48-1307 + GAGGAGACGGUUAAACAGGGCC 22 15266

HSV2-UL48-1308 + CG AGGAGACGGUUAAACAGGGCC 23 15267

HSV2-UL48-1309 + UCGAGGAGACGGU UAAACAGGGCC 24 15268

HSV2-UL48-1310 + UGGGACCCCCGGCUGGCC 18 15269

HSV2-UL48-1311 + UUGGGACCCCCGGCUGGCC 19 15270

HSV2-UL48-107 + CU UGGGACCCCCGGCUGGCC 20 5645

HSV2-UL48-1312 + UCUUGGGACCCCCGGCUGGCC 21 15271

HSV2-UL48-1313 + UUCUUGGGACCCCCGGCUGGCC 22 15272

HSV2-UL48-1314 + GUUCUUGGGACCCCCGGCUGGCC 23 15273

HSV2-UL48-1315 + UGUUCUUGGGACCCCCGGCUGGCC 24 15274

HSV2-UL48-1316 + UCGUCGACCAACAGGUCC 18 15275

HSV2-UL48-1317 + AUCGUCGACCAACAGGUCC 19 15276

HSV2-UL48-1318 + G AU CG U CG ACCA ACAG G U CC 20 15277

HSV2-UL48-1319 + AGAUCGUCGACCAACAGGUCC 21 15278

HSV2-UL48-1320 + CAGAUCGUCGACCAACAGGUCC 22 15279

HSV2-UL48-1321 + ACAGAUCGUCGACCAACAGGUCC 23 15280

HSV2-UL48-1322 + AACAGAUCG UCGACCAACAGGUCC 24 15281

HSV2-UL48-1323 + UCUAGCAUGGUACACAGC 18 15282

HSV2-UL48-1324 + AUCUAGCAUGGUACACAGC 19 15283 HSV2-UL48-718 + UAUCUAGCAUGGUACACAGC 20 6160

HSV2-UL48-1325 + GUAUCUAGCAUGGUACACAGC 21 15284

HSV2-UL48-1326 + GG UAUCUAGCAUGGUACACAGC 22 15285

HSV2-UL48-1327 + AGG UAUCUAGCAUGGUACACAGC 23 15286

HSV2-UL48-1328 + CAGG UAUCUAGCAUGGUACACAGC 24 15287

HSV2-UL48-1329 + ACG G U U A A AC AG G GCCGC 18 15288

HSV2-UL48-1330 + G ACG G U U A A AC AG G G CCG C 19 15289

HSV2-UL48-79 + AGACGGU UAAACAGGGCCGC 20 5569

HSV2-UL48-1331 + GAGACGGU UAAACAGGGCCGC 21 15290

HSV2-UL48-1332 + GGAGACGGUUAAACAGGGCCGC 22 15291

HSV2-UL48-1333 + AGGAGACGGUUAAACAGGGCCGC 23 15292

HSV2-UL48-1334 + GAGGAGACGGUUAAACAGGGCCGC 24 15293

HSV2-UL48-1335 + AUGUCGAUCGGGGAGCGC 18 15294

HSV2-UL48-1336 + AAUGUCGAUCGGGGAGCGC 19 15295

HSV2-UL48-728 + GAAUGUCGAUCGGGGAGCGC 20 6166

HSV2-UL48-1337 + CG AAUGUCGAUCGGGGAGCGC 21 15296

HSV2-UL48-1338 + GCG AAUGUCGAUCGGGGAGCGC 22 15297

HSV2-UL48-1339 + CGCG AAUGUCGAUCGGGGAGCGC 23 15298

HSV2-UL48-1340 + GCGCGAAUGUCGAUCGGGGAGCGC 24 15299

HSV2-UL48-1341 + GGGACGGGCAUGGGCGGC 18 15300

HSV2-UL48-1342 + GGGGACGGGCAUGGGCGGC 19 15301

HSV2-UL48-702 + GGGGGACGGGCAUGGGCGGC 20 6151

HSV2-UL48-1343 + GGGGGGACGGGCAUGGGCGGC 21 15302

HSV2-UL48-1344 + GGGGGGGACGGGCAUGGGCGGC 22 15303

HSV2-UL48-1345 + CGGGGGGGACGGGCAUGGGCGGC 23 15304

HSV2-UL48-1346 + GCGGGGGGGACGGGCAUGGGCGGC 24

15305

HSV2-UL48-1347 + GCCGCGGGGGGGACGGGC 18 15306

HSV2-UL48-1348 + GGCCGCGGGGGGGACGGGC 19 15307

HSV2-UL48-704 + GGGCCGCGGGGGGGACGGGC 20 6153

HSV2-UL48-1349 + AGGGCCGCGGGGGGGACGGGC 21 15308

HSV2-UL48-1350 + CAGGGCCGCGGGGGGGACGGGC 22 15309

HSV2-UL48-1351 + ACAGGGCCGCGGGGGGGACGGGC 23 15310

HSV2-UL48-1352 + AACAGGGCCGCGGGGGGGACGGGC 24 15311

HSV2-UL48-1353 + UUGGGACCCCCGGCUGGC 18 15312

HSV2-UL48-1354 + CUUGGGACCCCCGGCUGGC 19 15313

HSV2-UL48-692 + UCU UGGGACCCCCGGCUGGC 20 6145

HSV2-UL48-1355 + UUCUUGGGACCCCCGGCUGGC 21 15314

HSV2-UL48-1356 + GUUCUUGGGACCCCCGGCUGGC 22 15315

HSV2-UL48-1357 + UGUUCUUGGGACCCCCGGCUGGC 23 15316

HSV2-UL48-1358 + GUGU UCUUGGGACCCCCGGCUGGC 24 15317

HSV2-UL48-1359 + UGGGCGCGAAUGUCGAUC 18 15318

HSV2-UL48-1360 + GUGGGCGCGAAUGUCGAUC 19 15319

HSV2-UL48-57 + CGUGGGCGCGAAUGUCGAUC 20 5538

HSV2-UL48-1361 + CCGUGGGCGCGAAUGUCGAUC 21 15320

HSV2-UL48-1362 + GCCG UGGGCGCGAAUGUCGAUC 22 15321 HSV2-UL48-1363 + CGCCGUGGGCGCGAAUGUCGAUC 23 15322

HSV2-UL48-1364 + UCGCCGUGGGCGCGAAUGUCGAUC 24 15323

HSV2-UL48-1365 + GUCGAUCGGGGAGCGCUC 18 15324

HSV2-UL48-1366 + UGUCGAUCGGGGAGCGCUC 19 15325

HSV2-UL48-60 + AUGUCGAUCGGGGAGCGCUC 20 5541

HSV2-UL48-1367 + AAUGUCGAUCGGGGAGCGCUC 21 15326

HSV2-UL48-1368 + GAAUGUCGAUCGGGGAGCGCUC 22 15327

HSV2-UL48-1369 + CG AAUGUCGAUCGGGGAGCGCUC 23 15328

HSV2-UL48-1370 + GCGAAUGUCGAUCGGGGAGCGCUC 24 15329

HSV2-UL48-1371 + GCUGAAGCCCAGAUCGUC 18 15330

HSV2-UL48-1372 + CGCUGAAGCCCAGAUCGUC 19 15331

HSV2-UL48-714 + GCGCUGAAGCCCAGAUCGUC 20 6157

HSV2-UL48-1373 + CGCGCUGAAGCCCAGAUCGUC 21 15332

HSV2-UL48-1374 + CCGCGCUGAAGCCCAGAUCGUC 22 15333

HSV2-UL48-1375 + CCCGCGCUGAAGCCCAGAUCGUC 23 15334

HSV2-UL48-1376 + ACCCGCGCUGAAGCCCAGAUCGUC 24 15335

HSV2-UL48-1377 + CAUGGGGGCGGUUGGGUC 18 15336

HSV2-UL48-1378 + CCAUGGGGGCGGU UGGGUC 19 15337

HSV2-UL48-1144 + UCCAUGGGGGCGGU UGGGUC 20 15103

HSV2-UL48-1379 + GUCCAUGGGGGCGGUUGGGUC 21 15338

HSV2-UL48-1380 + GG UCCAUGGGGGCGGU UGGGUC 22 15339

HSV2-UL48-1381 + AGG UCCAUGGGGGCGGUUGGGUC 23 15340

HSV2-UL48-1382 + CAGGUCCAUGGGGGCGGU UGGGUC 24

15341

HSV2-UL48-1383 + UACAUGUCGGCGUUGG UC 18 15342

HSV2-UL48-1384 + GUACAUGUCGGCGUUGGUC 19 15343

HSV2-UL48-68 + GGUACAUGUCGGCGUUGGUC 20 5557

HSV2-UL48-1385 + CGGUACAUGUCGGCGU UGGUC 21 15344

HSV2-UL48-1386 + CCGGUACAUGUCGGCGU UGGUC 22 15345

HSV2-UL48-1387 + CCCGGUACAUGUCGGCGUUGGUC 23 15346

HSV2-UL48-1388 + UCCCGGUACAUGUCGGCGU UGGUC 24 15347

HSV2-UL48-1389 + GGGGCGGCUGGGGUGU UC 18 15348

HSV2-UL48-1390 + AGGGGCGGCUGGGGUGUUC 19 15349

HSV2-UL48-694 + GAGGGGCGGCUGGGGUGUUC 20 6146

HSV2-UL48-1391 + GGAGGGGCGGCUGGGGUGUUC 21 15350

HSV2-UL48-1392 + CGG AGGGGCGGCUGGGGUGUUC 22 15351

HSV2-UL48-1393 + GCGG AGGGGCGGCUGGGGUGUUC 23 15352

HSV2-UL48-1394 + AGCGGAGGGGCGGCUGGGGUGUUC 24

15353

HSV2-UL48-1395 + CCGACCGGUGGCGUACAG 18 15354

HSV2-UL48-1396 + GCCGACCGGUGGCGUACAG 19 15355

HSV2-UL48-95 + AGCCGACCGGUGGCGUACAG 20 5581

HSV2-UL48-1397 + CAGCCGACCGGUGGCGUACAG 21 15356

HSV2-UL48-1398 + UCAGCCGACCGGUGGCGUACAG 22 15357

HSV2-UL48-1399 + CUCAGCCGACCGGUGGCGUACAG 23 15358

HSV2-UL48-1400 + ACUCAGCCGACCGGUGGCGUACAG 24 15359 HSV2-UL48-1401 + GGGCGGCGAGGGCAUCAG 18 15360

HSV2-UL48-1402 + UGGGCGGCGAGGGCAUCAG 19 15361

HSV2-UL48-701 + AUGGGCGGCGAGGGCAUCAG 20 6150

HSV2-UL48-1403 + CAUGGGCGGCGAGGGCAUCAG 21 15362

HSV2-UL48-1404 + GCAUGGGCGGCGAGGGCAUCAG 22 15363

HSV2-UL48-1405 + GGCAUGGGCGGCGAGGGCAUCAG 23 15364

HSV2-UL48-1406 + GGGCAUGGGCGGCGAGGGCAUCAG 24 15365

HSV2-UL48-1407 + CGCGCCCGCAGCUCACCG 18 15366

HSV2-UL48-1408 + CCGCGCCCGCAGCUCACCG 19 15367

HSV2-UL48-41 + CCCGCGCCCGCAGCUCACCG 20 5615

HSV2-UL48-1409 + UCCCGCGCCCGCAGCUCACCG 21 15368

HSV2-UL48-1410 + CUCCCGCGCCCGCAGCUCACCG 22 15369

HSV2-UL48-1411 + CCUCCCGCGCCCGCAGCUCACCG 23 15370

HSV2-UL48-1412 + UCCUCCCGCGCCCGCAGCUCACCG 24 15371

HSV2-UL48-1413 + GACGGUUAAACAGGGCCG 18 15372

HSV2-UL48-1414 + AG ACG G U U A A AC AG G GCCG 19 15373

HSV2-UL48-78 + GAGACGGUUAAACAGGGCCG 20 5568

HSV2-UL48-1415 + GGAGACGGU UAAACAGGGCCG 21 15374

HSV2-UL48-1416 + AGGAGACGGU UAAACAGGGCCG 22 15375

HSV2-UL48-1417 + GAGGAGACGGUUAAACAGGGCCG 23 15376

HSV2-UL48-1418 + CGAGGAGACGGUUAAACAGGGCCG 24 15377

HSV2-UL48-1419 + CUAGCAUGGUACACAGCG 18 15378

HSV2-UL48-1420 + UCUAGCAUGGUACACAGCG 19 15379

HSV2-UL48-72 + AUCUAGCAUGGUACACAGCG 20 5624

HSV2-UL48-1421 + UAUCUAGCAUGGUACACAGCG 21 15380

HSV2-UL48-1422 + GU AUCUAGCAUGGUACACAGCG 22 15381

HSV2-UL48-1423 + GG UAUCUAGCAUGGUACACAGCG 23 15382

HSV2-UL48-1424 + AGGUAUCUAGCAUGGUACACAGCG 24 15383

HSV2-UL48-1425 + GACCGGUGGCGUACAGCG 18 15384

HSV2-UL48-1426 + CGACCGGUGGCGUACAGCG 19 15385

HSV2-UL48-698 + CCGACCGGUGGCGUACAGCG 20 6148

HSV2-UL48-1427 + GCCGACCGGUGGCGUACAGCG 21 15386

HSV2-UL48-1428 + AGCCGACCGGUGGCGUACAGCG 22 15387

HSV2-UL48-1429 + CAGCCGACCGGUGGCGUACAGCG 23 15388

HSV2-UL48-1430 + UCAGCCGACCGGUGGCGUACAGCG 24 15389

HSV2-UL48-1431 + CACAGCGCGGGACCCGCG 18 15390

HSV2-UL48-1432 + ACACAGCGCGGGACCCGCG 19 15391

HSV2-UL48-716 + UACACAGCGCGGGACCCGCG 20 6159

HSV2-UL48-1433 + GU ACACAGCGCGGGACCCGCG 21 15392

HSV2-UL48-1434 + GGUACACAGCGCGGGACCCGCG 22 15393

HSV2-UL48-1435 + UGG UACACAGCGCGGGACCCGCG 23 15394

HSV2-UL48-1436 + AUGG UACACAGCGCGGGACCCGCG 24 15395

HSV2-UL48-1437 + CGGUUAAACAGGGCCGCG 18 15396

HSV2-UL48-1438 + ACG G U U AAACAGG G CCG CG 19 15397

HSV2-UL48-80 + GACGGUUAAACAGGGCCGCG 20 5570

HSV2-UL48-1439 + AGACGGU UAAACAGGGCCGCG 21 15398 HSV2-UL48-1440 + GAGACGGU UAAACAGGGCCGCG 22 15399

HSV2-UL48-1441 + GGAGACGGU UAAACAGGGCCGCG 23 15400

HSV2-UL48-1442 + AGGAGACGGUUAAACAGGGCCGCG 24 15401

HSV2-UL48-1443 + GGGGGACGGGCAUGGGCG 18 15402

HSV2-UL48-1444 + GGGGGGACGGGCAUGGGCG 19 15403

HSV2-UL48-703 + GGGGGGGACGGGCAUGGGCG 20 6152

HSV2-UL48-1445 + CGGGGGGGACGGGCAUGGGCG 21 15404

HSV2-UL48-1446 + GCGGGGGGGACGGGCAUGGGCG 22 15405

HSV2-UL48-1447 + CGCGGGGGGGACGGGCAUGGGCG 23 15406

HSV2-UL48-1448 + CCGCGGGGGGGACGGGCAUGGGCG 24 15407

HSV2-UL48-1449 + GCGCUGAAGCCCAGAUCG 18 15408

HSV2-UL48-1450 + CGCGCUGAAGCCCAGAUCG 19 15409

HSV2-UL48-715 + CCGCGCUGAAGCCCAGAUCG 20 6158

HSV2-UL48-1451 + CCCGCGCUGAAGCCCAGAUCG 21 15410

HSV2-UL48-1452 + ACCCGCGCUGAAGCCCAGAUCG 22 15411

HSV2-UL48-1453 + GACCCGCGCUGAAGCCCAGAUCG 23 15412

HSV2-UL48-1454 + GG ACCCGCGCUGAAGCCCAGAUCG 24 15413

HSV2-UL48-1455 + GGGCGCGAAUGUCGAUCG 18 15414

HSV2-UL48-1456 + UGGGCGCGAAUGUCGAUCG 19 15415

HSV2-UL48-58 + GUGGGCGCGAAUGUCGAUCG 20 5539

HSV2-UL48-1457 + CG UGGGCGCGAAUGUCGAUCG 21 15416

HSV2-UL48-1458 + CCGUGGGCGCGAAUGUCGAUCG 22 15417

HSV2-UL48-1459 + GCCGUGGGCGCGAAUGUCGAUCG 23 15418

HSV2-UL48-1460 + CGCCGUGGGCGCGAAUGUCGAUCG 24 15419

HSV2-UL48-1461 + CCCCAGUCGAUCACG UCG 18 15420

HSV2-UL48-1462 + CCCCCAGUCGAUCACGUCG 19 15421

HSV2-UL48-725 + CCCCCCAGUCGAUCACG UCG 20 6165

HSV2-UL48-1463 + UCCCCCCAGUCGAUCACGUCG 21 15422

HSV2-UL48-1464 + AUCCCCCCAGUCGAUCACGUCG 22 15423

HSV2-UL48-1465 + CAUCCCCCCAGUCGAUCACGUCG 23 15424

HSV2-UL48-1466 + GCAUCCCCCCAG UCGAUCACGUCG 24 15425

HSV2-UL48-1467 + CUGAAGCCCAGAUCGUCG 18 15426

HSV2-UL48-1468 + GCUGAAGCCCAGAUCGUCG 19 15427

HSV2-UL48-74 + CGCUGAAGCCCAGAUCGUCG 20 5625

HSV2-UL48-1469 + GCGCUGAAGCCCAGAUCGUCG 21 15428

HSV2-UL48-1470 + CGCGCUGAAGCCCAGAUCGUCG 22 15429

HSV2-UL48-1471 + CCGCGCUGAAGCCCAGAUCGUCG 23 15430

HSV2-UL48-1472 + CCCGCGCUGAAGCCCAGAUCGUCG 24 15431

HSV2-UL48-1473 + UCGCGGGUGGCGGGCAGG 18 15432

HSV2-UL48-1474 + GUCGCGGGUGGCGGGCAGG 19 15433

HSV2-UL48-738 + CG UCGCGGGUGGCGGGCAGG 20 6170

HSV2-UL48-1475 + UCG UCGCGGGUGGCGGGCAGG 21 15434

HSV2-UL48-1476 + CUCG UCGCGGGUGGCGGGCAGG 22 15435

HSV2-UL48-1477 + GCUCG UCGCGGGUGGCGGGCAGG 23 15436

HSV2-UL48-1478 + AGCUCGUCGCGGGUGGCGGGCAGG 24 15437

HSV2-UL48-1479 + ACCGGUGGCGUACAGCGG 18 15438 HSV2-UL48-1480 + GACCGGUGGCGUACAGCGG 19 15439

HSV2-UL48-96 + CGACCGGUGGCGUACAGCGG 20 5638

HSV2-UL48-1481 + CCGACCGGUGGCGUACAGCGG 21 15440

HSV2-UL48-1482 + GCCGACCGGUGGCGUACAGCGG 22 15441

HSV2-UL48-1483 + AGCCGACCGGUGGCGUACAGCGG 23 15442

HSV2-UL48-1484 + CAGCCGACCGGUGGCGUACAGCGG 24 15443

HSV2-UL48-1485 + GGUUAAACAGGGCCGCGG 18 15444

HSV2-UL48-1486 + CGGUUAAACAGGGCCGCGG 19 15445

HSV2-UL48-81 + ACG G U U A A AC AG GGCCGCGG 20 5571

HSV2-UL48-1487 + GACGG UUAAACAGGGCCGCGG 21 15446

HSV2-UL48-1488 + AGACGGU UAAACAGGGCCGCGG 22 15447

HSV2-UL48-1489 + GAGACGGU UAAACAGGGCCGCGG 23 15448

HSV2-UL48-1490 + GGAGACGGU UAAACAGGGCCGCGG 24 15449

HSV2-UL48-1491 + CGUACAGCGGAGGGGCGG 18 15450

HSV2-UL48-1492 + GCGUACAGCGGAGGGGCGG 19 15451

HSV2-UL48-696 + GGCGUACAGCGGAGGGGCGG 20 6147

HSV2-UL48-1493 + UGGCGUACAGCGGAGGGGCGG 21 15452

HSV2-UL48-1494 + GUGGCGUACAGCGGAGGGGCGG 22 15453

HSV2-UL48-1495 + GG UGGCGUACAGCGGAGGGGCGG 23 15454

HSV2-UL48-1496 + CGGUGGCGUACAGCGGAGGGGCGG 24 15455

HSV2-UL48-1497 + CGCGGGUGGCGGGCAGGG 18 15456

HSV2-UL48-1498 + UCGCGGGUGGCGGGCAGGG 19 15457

HSV2-UL48-51 + GUCGCGGGUGGCGGGCAGGG 20 5617

HSV2-UL48-1499 + CGUCGCGGGUGGCGGGCAGGG 21 15458

HSV2-UL48-1500 + UCGUCGCGGGUGGCGGGCAGGG 22 15459

HSV2-UL48-1501 + CUCG UCGCGGGUGGCGGGCAGGG 23 15460

HSV2-UL48-1502 + GCUCGUCGCGGGUGGCGGGCAGGG 24 15461

HSV2-UL48-1503 + G U U A A AC AG GGCCGCGGG 18 15462

HSV2-UL48-1504 + GGUUAAACAGGGCCGCGGG 19 15463

HSV2-UL48-82 + CGGUUAAACAGGGCCGCGGG 20 5572

HSV2-UL48-1505 + ACGGU UAAACAGGGCCGCGGG 21 15464

HSV2-UL48-1506 + GACGG UUAAACAGGGCCGCGGG 22 15465

HSV2-UL48-1507 + AG ACG G U U A A AC AG G GCCGCGGG 23 15466

HSV2-UL48-1508 + GAGACGGU UAAACAGGGCCGCGGG 24 15467

HSV2-UL48-1509 + AACAGGGCCGCGGGGGGG 18 15468

HSV2-UL48-1510 + AAACAGGGCCGCGGGGGGG 19 15469

HSV2-UL48-705 + UAAACAGGGCCGCGGGGGGG 20 6154

HSV2-UL48-1511 + UU AAACAGGGCCGCGGGGGGG 21 15470

HSV2-UL48-1512 + GUUAAACAGGGCCGCGGGGGGG 22 15471

HSV2-UL48-1513 + GGU UAAACAGGGCCGCGGGGGGG 23 15472

HSV2-UL48-1514 + CGGUUAAACAGGGCCGCGGGGGGG 24 15473

HSV2-UL48-1515 + GGUACAUGUCGGCGUUGG 18 15474

HSV2-UL48-1516 + CGGUACAUGUCGGCGU UGG 19 15475

HSV2-UL48-723 + CCGGUACAUGUCGGCGU UGG 20 6163

HSV2-UL48-1517 + CCCGGUACAUGUCGGCG UUGG 21 15476

HSV2-UL48-1518 + UCCCGGUACAUGUCGGCGUUGG 22 15477 HSV2-UL48-1519 + CUCCCGGUACAUGUCGGCGU UGG 23 15478

HSV2-UL48-1520 + ACUCCCGGUACAUGUCGGCGUUGG 24 15479

HSV2-UL48-1521 + GGACCCCCGGCUGGCCUG 18 15480

HSV2-UL48-1522 + GGGACCCCCGGCUGGCCUG 19 15481

HSV2-UL48-109 + UGGGACCCCCGGCUGGCCUG 20 5647

HSV2-UL48-1523 + UUGGGACCCCCGGCUGGCCUG 21 15482

HSV2-UL48-1524 + CU UGGGACCCCCGGCUGGCCUG 22 15483

HSV2-UL48-1525 + UCUUGGGACCCCCGGCUGGCCUG 23 15484

HSV2-UL48-1526 + UUCUUGGGACCCCCGGCUGGCCUG 24 15485

HSV2-UL48-1527 + CUGGCCUGGGGGGCGGUG 18 15486

HSV2-UL48-1528 + GCUGGCCUGGGGGGCGGUG 19 15487

HSV2-UL48-688 + GGCUGGCCUGGGGGGCGGUG 20 6144

HSV2-UL48-1529 + CGGCUGGCCUGGGGGGCGGUG 21 15488

HSV2-UL48-1530 + CCGGCUGGCCUGGGGGGCGGUG 22 15489

HSV2-UL48-1531 + CCCGGCUGGCCUGGGGGGCGGUG 23 15490

HSV2-UL48-1532 + CCCCGGCUGGCCUGGGGGGCGGUG 24 15491

HSV2-UL48-1533 + CGGGUGGCGGGCAGGGUG 18 15492

HSV2-UL48-1534 + GCGGGUGGCGGGCAGGGUG 19 15493

HSV2-UL48-53 + CGCGGGUGGCGGGCAGGGUG 20 5619

HSV2-UL48-1535 + UCGCGGGUGGCGGGCAGGGUG 21 15494

HSV2-UL48-1536 + GUCGCGGGUGGCGGGCAGGGUG 22 15495

HSV2-UL48-1537 + CG UCGCGGGUGGCGGGCAGGGUG 23 15496

HSV2-UL48-1538 + UCGUCGCGGGUGGCGGGCAGGGUG 24

15497

HSV2-UL48-1539 + GGCAGCUCGUCGCGGGUG 18 15498

HSV2-UL48-1540 + AGGCAGCUCGUCGCGGG UG 19 15499

HSV2-UL48-740 + AAGGCAGCUCGUCGCGGGUG 20 6172

HSV2-UL48-1541 + GAAGGCAGCUCGUCGCGGGUG 21 15500

HSV2-UL48-1542 + CG AAGGCAGCUCGUCGCGGGUG 22 15501

HSV2-UL48-1543 + ACG AAGGCAGCUCGUCGCGGGUG 23 15502

HSV2-UL48-1544 + UACG AAGGCAGCUCGUCGCGGGUG 24 15503

HSV2-UL48-1545 + GUCGACCAACAGGUCCAU 18 15504

HSV2-UL48-1546 + CG U CG ACCAACAGG U CCA U 19 15505

HSV2-UL48-1146 + UCGUCGACCAACAGGUCCAU 20 15105

HSV2-UL48-1547 + AUCG UCG ACCAACAGG UCCAU 21 15506

HSV2-UL48-1548 + GAUCGUCGACCAACAGGUCCAU 22 15507

HSV2-UL48-1549 + AG AUCGUCG ACCAACAGG UCCAU 23 15508

HSV2-UL48-1550 + CAGAUCGUCGACCAACAGGUCCAU 24 15509

HSV2-UL48-1551 + GUGGGCGCGAAUGUCGAU 18 15510

HSV2-UL48-1552 + CGUGGGCGCGAAUGUCGAU 19 15511

HSV2-UL48-56 + CCGUGGGCGCGAAUGUCGAU 20 5537

HSV2-UL48-1553 + GCCGUGGGCGCGAAUGUCGAU 21 15512

HSV2-UL48-1554 + CGCCGUGGGCGCGAAUGUCGAU 22 15513

HSV2-UL48-1555 + UCGCCGUGGGCGCGAAUGUCGAU 23 15514

HSV2-UL48-1556 + GUCGCCG UGGGCGCGAAUGUCGAU 24 15515

HSV2-UL48-1557 + GGGACCCCCGGCUGGCCU 18 15516 HSV2-UL48-1558 + UGGGACCCCCGGCUGGCCU 19 15517

HSV2-UL48-108 + UUGGGACCCCCGGCUGGCCU 20 5646

HSV2-UL48-1559 + CUUGGGACCCCCGGCUGGCCU 21 15518

HSV2-UL48-1560 + UCUUGGGACCCCCGGCUGGCCU 22 15519

HSV2-UL48-1561 + U UCUUGGGACCCCCGGCUGGCCU 23 15520

HSV2-UL48-1562 + GUUCUUGGGACCCCCGGCUGGCCU 24 15521

HSV2-UL48-1563 + UGUCGAUCGGGGAGCGCU 18 15522

HSV2-UL48-1564 + AUGUCGAUCGGGGAGCGCU 19 15523

HSV2-UL48-59 + AAUGUCGAUCGGGGAGCGCU 20 5540

HSV2-UL48-1565 + GAAUGUCGAUCGGGGAGCGCU 21 15524

HSV2-UL48-1566 + CGAAUGUCGAUCGGGGAGCGCU 22 15525

HSV2-UL48-1567 + GCG AAUG UCGAUCGGGGAGCGCU 23 15526

HSV2-UL48-1568 + CGCGAAUGUCGAUCGGGGAGCGCU 24 15527

HSV2-UL48-1569 + GGGCGGCUGGGGUGUUCU 18 15528

HSV2-UL48-1570 + GGGGCGGCUGGGGUGU UCU 19 15529

HSV2-UL48-103 + AGGGGCGGCUGGGGUGUUCU 20 5642

HSV2-UL48-1571 + GAGGGGCGGCUGGGGUGUUCU 21 15530

HSV2-UL48-1572 + GGAGGGGCGGCUGGGGUGUUCU 22 15531

HSV2-UL48-1573 + CGG AGGGGCGGCUGGGGUGUUCU 23 15532

HSV2-UL48-1574 + GCGGAGGGGCGGCUGGGGUGUUCU 24

15533

HSV2-UL48-1575 + GCGCCAUGGCCUCGUAG U 18 15534

HSV2-UL48-1576 + UGCGCCAUGGCCUCGUAGU 19 15535

HSV2-UL48-742 + CUGCGCCAUGGCCUCGUAGU 20 6174

HSV2-UL48-1577 + ACUGCGCCAUGGCCUCGUAGU 21 15536

HSV2-UL48-1578 + AACUGCGCCAUGGCCUCGUAGU 22 15537

HSV2-UL48-1579 + AAACUGCGCCAUGGCCUCGUAGU 23 15538

HSV2-UL48-1580 + AAAACUGCGCCAUGGCCUCGUAGU 24 15539

HSV2-UL48-1581 + GCGGGUGGCGGGCAGGGU 18 15540

HSV2-UL48-1582 + CGCGGGUGGCGGGCAGGG U 19 15541

HSV2-UL48-52 + UCGCGGGUGGCGGGCAGGGU 20 5618

HSV2-UL48-1583 + GUCGCGGGUGGCGGGCAGGGU 21 15542

HSV2-UL48-1584 + CG UCGCGGGUGGCGGGCAGGGU 22 15543

HSV2-UL48-1585 + UCGUCGCGGGUGGCGGGCAGGGU 23 15544

HSV2-UL48-1586 + CUCGUCGCGGGUGGCGGGCAGGGU 24 15545

HSV2-UL48-1587 + CCAUGGGGGCGGUUGGGU 18 15546

HSV2-UL48-1588 + UCCAUGGGGGCGGUUGGGU 19 15547

HSV2-UL48-1589 + GUCCAUGGGGGCGGUUGGGU 20 15548

HSV2-UL48-1590 + GG UCCAUGGGGGCGGU UGGGU 21 15549

HSV2-UL48-1591 + AGG UCCAUGGGGGCGGU UGGGU 22 15550

HSV2-UL48-1592 + CAGG UCCAUGGGGGCGGUUGGGU 23 15551

HSV2-UL48-1593 + ACAGGUCCAUGGGGGCGGUUGGGU 24

15552

HSV2-UL48-1594 + GUACAUGUCGGCGUUGGU 18 15553

HSV2-UL48-1595 + GGUACAUGUCGGCGUUGGU 19 15554

HSV2-UL48-67 + CGGUACAUGUCGGCGU UGGU 20 5556 HSV2-UL48-1596 + CCGGUACAUGUCGGCGUUGGU 21 15555

HSV2-UL48-1597 + CCCGGUACAUGUCGGCG UUGGU 22 15556

HSV2-UL48-1598 + UCCCGGUACAUGUCGGCGUUGGU 23 15557

HSV2-UL48-1599 + CUCCCGGUACAUGUCGGCGUUGGU 24 15558

HSV2-UL48-1600 - GCCCAGCGACGUGAUCGA 18 15559

HSV2-UL48-1601 - UGCCCAGCGACGUGAUCGA 19 15560

HSV2-UL48-635 - CUGCCCAGCGACGUGAUCGA 20 6111

HSV2-UL48-1602 - GCUGCCCAGCGACGUGAUCGA 21 15561

HSV2-UL48-1603 - CGCUGCCCAGCGACGUGAUCGA 22 15562

HSV2-UL48-1604 - ACGCUGCCCAGCGACGUGAUCGA 23 15563

HSV2-UL48-1605 - GACGCUGCCCAGCGACGUGAUCGA 24 15564

HSV2-UL48-1606 - CCAUGCUAGAUACCUGGA 18 15565

HSV2-UL48-1607 - ACCAUGCUAGAUACCUGGA 19 15566

HSV2-UL48-629 - UACCAUGCUAGAUACCUGGA 20 6107

HSV2-UL48-1608 - GU ACCAUGCUAGAUACCUGGA 21 15567

HSV2-UL48-1609 - UGUACCAUGCUAGAUACCUGGA 22 15568

HSV2-UL48-1610 - GUG UACCAUGCUAGAUACCUGGA 23 15569

HSV2-UL48-1611 - UGUGUACCAUGCUAGAUACCUGGA 24 15570

HSV2-UL48-1612 - GGCGCGGGAGGAGAGCUA 18 15571

HSV2-UL48-1613 - GGGCGCGGGAGGAGAGCUA 19 15572

HSV2-UL48-651 - CGGGCGCGGGAGGAGAGCUA 20 6120

HSV2-UL48-1614 - GCGGGCGCGGGAGGAGAGCUA 21 15573

HSV2-UL48-1615 - UGCGGGCGCGGGAGGAGAGCUA 22 15574

HSV2-UL48-1616 - CUGCGGGCGCGGGAGGAGAGCUA 23 15575

HSV2-UL48-1617 - GCUGCGGGCGCGGGAGGAGAGCUA 24 15576

HSV2-UL48-1618 - GACCAACGCCGACAUGUA 18 15577

HSV2-UL48-1619 - CG ACCAACGCCG ACAUG U A 19 15578

HSV2-UL48-632 - CCGACCAACGCCGACAUGUA 20 6110

HSV2-UL48-1620 - CCCG ACCAACGCCG ACAUG U A 21 15579

HSV2-UL48-1621 - UCCCGACCAACGCCGACAUGUA 22 15580

HSV2-UL48-1622 - UUCCCGACCAACGCCGACAUGUA 23 15581

HSV2-UL48-1623 - GUUCCCGACCAACGCCGACAUGUA 24 15582

HSV2-UL48-1624 - AUGCUAGAUACCUGGAAC 18 15583

HSV2-UL48-1625 - CAUGCUAGAUACCUGGAAC 19 15584

HSV2-UL48-630 - CCAUGCUAGAUACCUGGAAC 20 6108

HSV2-UL48-1626 - ACCAUGCUAGAUACCUGGAAC 21 15585

HSV2-UL48-1627 - UACCAUGCUAGAUACCUGGAAC 22 15586

HSV2-UL48-1628 - GUACCAUGCUAGAUACCUGGAAC 23 15587

HSV2-UL48-1629 - UGUACCAUGCUAGAUACCUGGAAC 24 15588

HSV2-UL48-1630 - CCCAGCGACGUGAUCGAC 18 15589

HSV2-UL48-1631 - GCCCAGCGACGUGAUCGAC 19 15590

HSV2-UL48-25 - UGCCCAGCGACGUGAUCGAC 20 5501

HSV2-UL48-1632 - CUGCCCAGCGACGUGAUCGAC 21 15591

HSV2-UL48-1633 - GCUGCCCAGCGACGUGAUCGAC 22 15592

HSV2-UL48-1634 - CGCUGCCCAGCGACGUGAUCGAC 23 15593

HSV2-UL48-1635 - ACGCUGCCCAGCGACG UGAUCGAC 24 15594 HSV2-UL48-1636 - GACGAUCUGUUUGCGGAC 18 15595

HSV2-UL48-1637 - CGACGAUCUGU UUGCGGAC 19 15596

HSV2-UL48-617 - UCGACGAUCUGUUUGCGGAC 20 6099

HSV2-UL48-1638 - GUCGACGAUCUGUUUGCGGAC 21 15597

HSV2-UL48-1639 - GGUCGACGAUCUGUUUGCGGAC 22 15598

HSV2-UL48-1640 - UGGUCGACGAUCUGUU UGCGGAC 23 15599

HSV2-UL48-1641 - UUGGUCGACGAUCUGU UUGCGGAC 24 15600

HSV2-UL48-1642 - GUGUACCAUGCUAGAUAC 18 15601

HSV2-UL48-1643 - UGUGUACCAUGCUAGAUAC 19 15602

HSV2-UL48-627 - CUGUGUACCAUGCUAGAUAC 20 6106

HSV2-UL48-1644 - GCUGUGUACCAUGCUAGAUAC 21 15603

HSV2-UL48-1645 - CGCUGUGUACCAUGCUAGAUAC 22 15604

HSV2-UL48-1646 - GCGCUGUGUACCAUGCUAGAUAC 23 15605

HSV2-UL48-1647 - CGCGCUGUGUACCAUGCUAGAUAC 24 15606

HSV2-UL48-1648 - ACCAACGCCGACAUGUAC 18 15607

HSV2-UL48-1649 - GACCAACGCCGACAUGUAC 19 15608

HSV2-UL48-23 - CG ACCAACGCCGACAUGUAC 20 5607

HSV2-UL48-1650 - CCG ACCAACGCCGACAUGUAC 21 15609

HSV2-UL48-1651 - CCCGACCAACGCCGACAUGUAC 22 15610

HSV2-UL48-1652 - UCCCGACCAACGCCGACAUGUAC 23 15611

HSV2-UL48-1653 - UUCCCG ACCAACGCCGACAUG UAC 24 15612

HSV2-UL48-1654 - UGUACCAUGCUAGAUACC 18 15613

HSV2-UL48-1655 - GUGUACCAUGCUAGAUACC 19 15614

HSV2-UL48-19 - UGUGUACCAUGCUAGAUACC 20 5604

HSV2-UL48-1656 - CUGUGUACCAUGCUAGAUACC 21 15615

HSV2-UL48-1657 - GCUGUGUACCAUGCUAGAUACC 22 15616

HSV2-UL48-1658 - CGCUGUGUACCAUGCUAGAUACC 23 15617

HSV2-UL48-1659 - GCGCUGUGUACCAUGCUAGAUACC 24 15618

HSV2-UL48-1660 - CCGGACCCAACCGCCCCC 18 15619

HSV2-UL48-1661 - CCCGGACCCAACCGCCCCC 19 15620

HSV2-UL48-1662 - U CCCG G ACCC AACCG CCCCC 20 15621

HSV2-UL48-1663 - U U CCCG G ACCCAACCG CCCCC 21 15622

HSV2-UL48-1664 - AUUCCCGGACCCAACCGCCCCC 22 15623

HSV2-UL48-1665 - UAUUCCCGGACCCAACCGCCCCC 23 15624

HSV2-UL48-1666 - UU AU UCCCGGACCCAACCGCCCCC 24 15625

HSV2-UL48-1667 - AGGCCAGCCGGGGGUCCC 18 15626

HSV2-UL48-1668 - CAGGCCAGCCGGGGGUCCC 19 15627

HSV2-UL48-623 - CCAGGCCAGCCGGGGGUCCC 20 6102

HSV2-UL48-1669 - CCCAGGCCAGCCGGGGGUCCC 21 15628

HSV2-UL48-1670 - CCCCAGGCCAGCCGGGGGUCCC 22 15629

HSV2-UL48-1671 - CCCCCAGGCCAGCCGGGGGUCCC 23 15630

HSV2-UL48-1672 - CCCCCCAGGCCAGCCGGGGGUCCC 24 15631

HSV2-UL48-1673 - GGGGGGAUGCGCACGUCC 18 15632

HSV2-UL48-1674 - UGGGGGGAUGCGCACGUCC 19 15633

HSV2-UL48-640 - CUGGGGGGAUGCGCACGUCC 20 6112

HSV2-UL48-1675 - ACUGGGGGGAUGCGCACGUCC 21 15634 HSV2-UL48-1676 - GACUGGGGGGAUGCGCACGUCC 22 15635

HSV2-UL48-1677 - CGACUGGGGGGAUGCGCACGUCC 23 15636

HSV2-UL48-1678 - UCGACUGGGGGGAUGCGCACGUCC 24 15637

HSV2-UL48-1679 - ACCGCCCCCCAGGCCAGC 18 15638

HSV2-UL48-1680 - CACCG CCCCCCAG GCCAG C 19 15639

HSV2-UL48-7 - CCACCGCCCCCCAGGCCAGC 20 5597

HSV2-UL48-1681 - GCCACCGCCCCCCAGGCCAGC 21 15640

HSV2-UL48-1682 - CG CCACCG CCCCCCAG GCCAG C 22 15641

HSV2-UL48-1683 - U CGCCACCG CCCCCCAG G CCAG C 23 15642

HSV2-UL48-1684 - U U CG CCACCG CCCCCCAG G CCAG C 24 15643

HSV2-UL48-1685 - GCGGUGAGCUGCGGGCGC 18 15644

HSV2-UL48-1686 - CGCGGUGAGCUGCGGGCGC 19 15645

HSV2-UL48-38 - CCGCGGUGAGCUGCGGGCGC 20 5612

HSV2-UL48-1687 - UCCGCGG UGAGCUGCGGGCGC 21 15646

HSV2-UL48-1688 - UUCCGCGGUGAGCUGCGGGCGC 22 15647

HSV2-UL48-1689 - UU UCCGCGGUGAGCUGCGGGCGC 23 15648

HSV2-UL48-1690 - UUU UCCGCGGUGAGCUGCGGGCGC 24 15649

HSV2-UL48-1691 - CCGCGGUGAGCUGCGGGC 18 15650

HSV2-UL48-1692 - UCCGCGG UGAGCUGCGGGC 19 15651

HSV2-UL48-645 - UUCCGCGGUGAGCUGCGGGC 20 6117

HSV2-UL48-1693 - UUUCCGCGGUGAGCUGCGGGC 21 15652

HSV2-UL48-1694 - U UUUCCGCGGUGAGCUGCGGGC 22 15653

HSV2-UL48-1695 - UUU UUCCGCGGUGAGCUGCGGGC 23 15654

HSV2-UL48-1696 - GUUUU UCCGCGGUGAGCUGCGGGC 24 15655

HSV2-UL48-1697 - CACCG CCCCCC AGG CCAG 18 15656

HSV2-UL48-1698 - CCACCG CCCCCCAG G CCAG 19 15657

HSV2-UL48-620 - G CCACCG CCCCCCAG G CCAG 20 6101

HSV2-UL48-1699 - CGCCACCGCCCCCCAGGCCAG 21 15658

HSV2-UL48-1700 - UCGCCACCGCCCCCCAGGCCAG 22 15659

HSV2-UL48-1701 - U U CG CCACCGCCCCCCAG G CCAG 23 15660

HSV2-UL48-1702 - U U U CG CCACCGCCCCCCAG G CCAG 24 15661

HSV2-UL48-1703 - GAGCUGCGGGCGCGGGAG 18 15662

HSV2-UL48-1704 - UGAGCUGCGGGCGCGGGAG 19 15663

HSV2-UL48-650 - GUGAGCUGCGGGCGCGGGAG 20 6119

HSV2-UL48-1705 - GG UGAGCUGCGGGCGCGGGAG 21 15664

HSV2-UL48-1706 - CGG UGAGCUGCGGGCGCGGGAG 22 15665

HSV2-UL48-1707 - GCGG UGAGCUGCGGGCGCGGGAG 23 15666

HSV2-UL48-1708 - CGCGGUGAGCUGCGGGCGCGGGAG 24 15667

HSV2-UL48-1709 - CCCUGCCCGCCACCCGCG 18 15668

HSV2-UL48-1710 - ACCCUGCCCGCCACCCGCG 19 15669

HSV2-UL48-641 - CACCCUG CCCG CCACCCG CG 20 6113

HSV2-UL48-1711 - CCACCCUGCCCGCCACCCGCG 21 15670

HSV2-UL48-1712 - CCCACCCUGCCCGCCACCCGCG 22 15671

HSV2-UL48-1713 - CCCCACCCUGCCCGCCACCCGCG 23 15672

HSV2-UL48-1714 - UCCCCACCCUGCCCGCCACCCGCG 24 15673

HSV2-UL48-1715 - UGGCGCAGUU UUUCCGCG 18 15674 HSV2-UL48-1716 - AUGGCGCAGUUU UUCCGCG 19 15675

HSV2-UL48-643 - CAUGGCGCAGUU UUUCCGCG 20 6115

HSV2-UL48-1717 - CCAUGGCGCAGUU UUUCCGCG 21 15676

HSV2-UL48-1718 - GCCAUGGCGCAGUU UU UCCGCG 22 15677

HSV2-UL48-1719 - GGCCAUGGCGCAGUUU UUCCGCG 23 15678

HSV2-UL48-1720 - AGGCCAUGGCGCAGUUU UUCCGCG 24 15679

HSV2-UL48-1721 - CGCGGUGAGCUGCGGGCG 18 15680

HSV2-UL48-1722 - CCGCGGUGAGCUGCGGGCG 19 15681

HSV2-UL48-37 - UCCGCGG UGAGCUGCGGGCG 20 5611

HSV2-UL48-1723 - UUCCGCGGUGAGCUGCGGGCG 21 15682

HSV2-UL48-1724 - U UUCCGCGGUGAGCUGCGGGCG 22 15683

HSV2-UL48-1725 - UUU UCCGCGGUGAGCUGCGGGCG 23 15684

HSV2-UL48-1726 - UUU UUCCGCGGUGAGCUGCGGGCG 24 15685

HSV2-UL48-1727 - AUCUGUUUGCGGACGCGG 18 15686

HSV2-UL48-1728 - GAUCUGU UUGCGGACGCGG 19 15687

HSV2-UL48-618 - CGAUCUGUU UGCGGACGCGG 20 6100

HSV2-UL48-1729 - ACGAUCUGUUUGCGGACGCGG 21 15688

HSV2-UL48-1730 - GACGAUCUGUUUGCGGACGCGG 22 15689

HSV2-UL48-1731 - CGACGAUCUGU UUGCGGACGCGG 23 15690

HSV2-UL48-1732 - UCGACGAUCUG UUUGCGGACGCGG 24 15691

HSV2-UL48-1733 - GGUGAGCUGCGGGCGCGG 18 15692

HSV2-UL48-1734 - CGGUGAGCUGCGGGCGCGG 19 15693

HSV2-UL48-648 - GCGGUGAGCUGCGGGCGCGG 20 6118

HSV2-UL48-1735 - CGCGGUGAGCUGCGGGCGCGG 21 15694

HSV2-UL48-1736 - CCGCGGUGAGCUGCGGGCGCGG 22 15695

HSV2-UL48-1737 - UCCGCGGUGAGCUGCGGGCGCGG 23 15696

HSV2-UL48-1738 - UUCCGCGGUGAGCUGCGGGCGCGG 24 15697

HSV2-UL48-1739 - GUGAGCUGCGGGCGCGGG 18 15698

HSV2-UL48-1740 - GGUGAGCUGCGGGCGCGGG 19 15699

HSV2-UL48-39 - CGGUGAGCUGCGGGCGCGGG 20 5613

HSV2-UL48-1741 - GCGGUGAGCUGCGGGCGCGGG 21 15700

HSV2-UL48-1742 - CGCGGUGAGCUGCGGGCGCGGG 22 15701

HSV2-UL48-1743 - CCGCGGUGAGCUGCGGGCGCGGG 23 15702

HSV2-UL48-1744 - UCCGCGG UGAGCUGCGGGCGCGGG 24 15703

HSV2-UL48-1745 - CAGCGACGUGAUCGACUG 18 15704

HSV2-UL48-1746 - CCAGCGACGUGAUCGACUG 19 15705

HSV2-UL48-27 - CCCAGCGACGUGAUCGACUG 20 5609

HSV2-UL48-1747 - GCCCAGCGACGUGAUCGACUG 21 15706

HSV2-UL48-1748 - UGCCCAGCGACGUGAUCGACUG 22 15707

HSV2-UL48-1749 - CUGCCCAGCGACGUGAUCGACUG 23 15708

HSV2-UL48-1750 - GCUGCCCAGCGACGUGAUCGACUG 24 15709

HSV2-UL48-1751 - AACCGUCUCCUCGACGAU 18 15710

HSV2-UL48-1752 - UAACCGUCUCCUCGACGAU 19 15711

HSV2-UL48-625 - UUAACCGUCUCCUCGACGAU 20 6104

HSV2-UL48-1753 - UUUAACCGUCUCCUCGACGAU 21 15712

HSV2-UL48-1754 - GUU UAACCGUCUCCUCGACGAU 22 15713 HSV2-UL48-1755 - UGU UUAACCGUCUCCUCGACGAU 23 15714

HSV2-UL48-1756 - CUGUUUAACCGUCUCCUCGACGAU 24 15715

HSV2-UL48-1757 - CCAGCGACGUGAUCGACU 18 15716

HSV2-UL48-1758 - CCCAGCGACGUGAUCGACU 19 15717

HSV2-UL48-26 - GCCCAGCGACGUGAUCGACU 20 5608

HSV2-UL48-1759 - UGCCCAGCGACGUGAUCGACU 21 15718

HSV2-UL48-1760 - CUGCCCAGCGACGUGAUCGACU 22 15719

HSV2-UL48-1761 - GCUGCCCAGCGACGUGAUCGACU 23 15720

HSV2-UL48-1762 - CGCUGCCCAGCGACGUGAUCGACU 24 15721

HSV2-UL48-1763 - ACGAGCUGCCUUCGUACU 18 15722

HSV2-UL48-1764 - GACGAGCUGCCUUCGUACU 19 15723

HSV2-UL48-642 - CGACGAGCUGCCU UCGUACU 20 6114

HSV2-UL48-1765 - GCGACGAGCUGCCUUCGUACU 21 15724

HSV2-UL48-1766 - CGCGACGAGCUGCCUUCGUACU 22 15725

HSV2-UL48-1767 - CCGCGACGAGCUGCCU UCGUACU 23 15726

HSV2-UL48-1768 - CCCGCGACGAGCUGCCU UCGUACU 24 15727

HSV2-UL48-1769 - GUUUUUCCGCGGUGAGCU 18 15728

HSV2-UL48-1770 - AGU UU UUCCGCGGUGAGCU 19 15729

HSV2-UL48-644 - CAGU UUUUCCGCGGUGAGCU 20 6116

HSV2-UL48-1771 - GCAGUUU UUCCGCGGUGAGCU 21 15730

HSV2-UL48-1772 - CGCAGU UUU UCCGCGGUGAGCU 22 15731

HSV2-UL48-1773 - GCGCAGUUUU UCCGCGGUGAGCU 23 15732

HSV2-UL48-1774 - GGCGCAGUUU UUCCGCGGUGAGCU 24 15733

HSV2-UL48-1775 - UUGGUCGACGAUCUGUUU 18 15734

HSV2-UL48-1776 - GUUGGUCGACGAUCUGUUU 19 15735

HSV2-UL48-616 - UGUUGGUCGACGAUCUGUUU 20 6098

HSV2-UL48-1777 - CUGUUGGUCGACGAUCUGU UU 21 15736

HSV2-UL48-1778 - CCUGUUGGUCGACGAUCUGUUU 22 15737

HSV2-UL48-1779 - ACCUGU UGGUCGACGAUCUGUU U 23 15738

HSV2-UL48-1780 - GACCUGUUGGUCGACGAUCUGU UU 24 15739

Table 12E provides exemplary targeting domains for knocking out the UL48 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene), and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 12E

Strand Site

Length

HSV2-UL48-1781 + GCUCGGCGUACGCGGCCCA 19 15740

HSV2-UL48-322 + GUCCAAAGCCCCAUACGAGA 20 5804

HSV2-U L48-1782 + GGGGUCGUGAGGGGCGCC 18 15741

HSV2-UL48-1001 + GCGGGGUCGUGAGGGGCGCC 20 6338

HSV2-UL48-1783 + GGCGGGGUCGUGAGGGGCGCC 21 15742

HSV2-U L48-1784 + GGGCGGGGUCGUGAGGGGCGCC 22 15743

HSV2-UL48-366 + GCCCUCCGAGGUCGCGACGC 20 5848

HSV2-U L48-1785 + GUACGAG UCCAACUUGGC 18 15744

HSV2-U L48-1013 + GAGUACGAGUCCAACUUGGC 20 6347

HSV2-U L48-1786 + GGAGUACGAGUCCAACUUGGC 21 15745

HSV2-U L48-1787 + GCUGGAGUACGAGUCCAACU UGGC 24 15746

HSV2-UL48-1788 + GCGGGAGGUUCAGGUGCUC 19 15747

HSV2-UL48-1049 + GCCCCAUACGAGACGGGGUC 20 6367

HSV2-U L48-1789 + GUUGCCCUGCAGGACGGG 18 15748

HSV2-UL48-1790 + GGUUGCCCUGCAGGACGGG 19 15749

HSV2-U L48-1791 + GCCUGGUUGCCCUGCAGGACGGG 23 15750

HSV2-U L48-1792 + GGCCUGGU UGCCCUGCAGGACGGG 24 15751

HSV2-UL48-1793 + GAGGUCGCGACGCUGGAGU 19 15752

HSV2-U L48-1794 - GUUUCAGCCCCUGAUGU UUAUCA 23 15753

HSV2-UL48-307 - GACG UGGAGUCCCCCUCCCC 20 5789

HSV2-UL48-1795 - GGACGUGGAGUCCCCCUCCCC 21 15754

HSV2-U L48-1796 - GGGACGUGGAGUCCCCCUCCCC 22 15755

HSV2-UL48-1797 - GGGGACGUGGAGUCCCCCUCCCC 23 15756

HSV2-UL48-1798 - GGGGGACGUGGAGUCCCCCUCCCC 24 15757

HSV2-U L48-1799 - GAACUCCGCCUGGACGGCGAGGAG 24 15758

HSV2-UL48-1800 - GACU UGGAGAUGCUGGGGGACG 22 15759

HSV2-U L48-1801 - GCGUCGCGACCUCGGAGG 18 15760

HSV2-U L48-1802 - GCAU UGACGACU UUGGGGGG 20 15761

HSV2-UL48-1803 - GGCAUUGACGACU UUGGGGGG 21 15762

HSV2-U L48-1804 - GGGCAUUGACGACUUUGGGGGG 22 15763

HSV2-U L48-1805 - GCCAUGGGCAU UGACGACUUUG 22 15764

HSV2-UL48-1806 - GCAACCAGGCCCGCUCCU 18 15765

HSV2-U L48-1807 - GGCAACCAGGCCCGCUCCU 19 15766

HSV2-UL48-836 - GGGCAACCAGGCCCGCUCCU 20 6229

HSV2-UL48-1808 - GCAGGGCAACCAGGCCCGCUCCU 23 15767

HSV2-U L48-1809 - GGCGG ACCAG AAACAAU U 18 15768

HSV2-UL48-1810 - GGGCGG ACCAG AAACAAU U 19 15769

HSV2-U L48-1811 - GCGGGCGG ACCAG AAACAAU U 21 15770

HSV2-U L48-1812 - GCCGCGGGCGG ACCAG AAACAAU U 24 15771

HSV2-UL48-1813 - GCU UUGGACGUGGACGAU U 19 15772

HSV2-UL48-883 - GGCUUUGGACGUGGACGAUU 20 6260

HSV2-U L48-1814 - GGGCUU UGGACGUGGACGAUU 21 15773

HSV2-UL48-1815 - GGGGCUUUGGACGUGGACGAUU 22 15774 Table 12F provides exemplary targeting domains for knocking out the UL48 gene selected according to the six tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 12F

HSV2-UL48-1843 + CACCAGCGGGAGGUUCAGGUGCUC 24 15802

HSV2-UL48-1844 + CCCAUACGAGACGGGGUC 18 15803

HSV2-UL48-1845 + CCCCAUACGAGACGGGGUC 19 15804

HSV2-UL48-1846 + AGCCCCAUACGAGACGGGGUC 21 15805

HSV2-U L48-1847 + AAGCCCCAUACGAGACGGGGUC 22 15806

HSV2-U L48-1848 + AAAGCCCCAUACGAGACGGGGUC 23 15807

HSV2-UL48-1849 + CAAAGCCCCAUACGAGACGGGGUC 24 15808

HSV2-U L48-1850 + ACCUCCUCGCCGUCCAGG 18 15809

HSV2-U L48-1851 + CACCUCCUCGCCGUCCAGG 19 15810

HSV2-UL48-340 + CCACCUCCUCGCCGUCCAGG 20 5822

HSV2-U L48-1852 + UCCACCUCCUCGCCGUCCAGG 21 15811

HSV2-UL48-1853 + AUCCACCUCCUCGCCGUCCAGG 22 15812

HSV2-UL48-1854 + UAUCCACCUCCUCGCCGUCCAGG 23 15813

HSV2-U L48-1855 + AUAUCCACCUCCUCGCCGUCCAGG 24 15814

HSV2-UL48-376 + UGGUUGCCCUGCAGGACGGG 20 5858

HSV2-U L48-1856 + CUGGUUGCCCUGCAGGACGGG 21 15815

HSV2-U L48-1857 + CCUGGUUGCCCUGCAGGACGGG 22 15816

HSV2-UL48-1858 + AGGUCGCGACGCUGGAGU 18 15817

HSV2-U L48-1014 + CGAGGUCGCGACGCUGGAGU 20 6348

HSV2-UL48-1859 + CCG AGGUCGCGACGCUGGAGU 21 15818

HSV2-UL48-1860 + UCCG AGGUCGCGACGCUGGAGU 22 15819

HSV2-U L48-1861 + CUCCG AGGUCGCGACGCUGGAGU 23 15820

HSV2-UL48-1862 + CCUCCGAGGUCGCGACGCUGGAGU 24 15821

HSV2-UL48-1863 - AGCCCCUGAUGU UUAUCA 18 15822

HSV2-U L48-1864 - CAGCCCCUGAUGUUUAUCA 19 15823

HSV2-UL48-816 - UCAGCCCCUGAUGU U UAUCA 20 6218

HSV2-U L48-1865 - U UCAGCCCCUGAUGUU UAUCA 21 15824

HSV2-U L48-1866 - U UUCAGCCCCUGAUGU UUAUCA 22 15825

HSV2-UL48-1867 - UGUUUCAGCCCCUGAUG UUUAUCA 24 15826

HSV2-U L48-1868 - CGUGGAGUCCCCCUCCCC 18 15827

HSV2-U L48-1869 - ACGUGGAGUCCCCCUCCCC 19 15828

HSV2-UL48-1870 - CGCCUGGACGGCGAGGAG 18 15829

HSV2-U L48-1871 - CCGCCUGGACGGCGAGGAG 19 15830

HSV2-UL48-866 - UCCGCCUGGACGGCGAGGAG 20 6251

HSV2-UL48-1872 - CUCCGCCUGGACGGCGAGGAG 21 15831

HSV2-U L48-1873 - ACUCCGCCUGGACGGCGAGGAG 22 15832

HSV2-UL48-1874 - AACUCCGCCUGGACGGCGAGGAG 23 15833

HSV2-U L48-1875 - UGGAGAUGCUGGGGGACG 18 15834

HSV2-U L48-1876 - UUGGAGAUGCUGGGGGACG 19 15835

HSV2-UL48-305 - CU UGGAGAUGCUGGGGGACG 20 5787

HSV2-UL48-1877 - ACU UGGAGAUGCUGGGGGACG 21 15836

HSV2-UL48-1878 - CGACU UGGAGAUGCUGGGGGACG 23 15837

HSV2-UL48-1879 - UCGACUUGGAGAUGCUGGGGGACG 24

15838

HSV2-U L48-1880 - CGGAUCGCUCACCGUGCG 18 15839

HSV2-UL48-1881 - ACGGAUCGCUCACCGUGCG 19 15840 HSV2-UL48-250 - AACGGAUCGCUCACCGUGCG 20 5732

HSV2-UL48-1882 - CAACGGAUCGCUCACCGUGCG 21 15841

HSV2-UL48-1883 - U CAACGGAU CG C U CACCG UGCG 22 15842

HSV2-UL48-1884 - AUCAACGGAUCGCUCACCGUGCG 23 15843

HSV2-U L48-1885 - UAUCAACGGAUCGCUCACCGUGCG 24 15844

HSV2-U L48-1886 - AGCGUCGCGACCUCGGAGG 19 15845

HSV2-UL48-842 - CAGCGUCGCGACCUCGGAGG 20 6234

HSV2-U L48-1887 - CCAGCGUCGCGACCUCGGAGG 21 15846

HSV2-U L48-1888 - UCCAGCGUCGCGACCUCGGAGG 22 15847

HSV2-UL48-1889 - CUCCAGCGUCGCGACCUCGGAGG 23 15848

HSV2-U L48-1890 - ACUCCAGCGUCGCGACCUCGGAGG 24 15849

HSV2-UL48-1891 - AUUGACGACUUUGGGGGG 18 15850

HSV2-UL48-1892 - CAU UGACGACUU UGGGGGG 19 15851

HSV2-U L48-1893 - UGGGCAUUGACGACUU UGGGGGG 23 15852

HSV2-UL48-1894 - AUGGGCAUUGACGACU UUGGGGGG 24

15853

HSV2-U L48-1895 - UGGGCAUUGACGACUU UG 18 15854

HSV2-U L48-1896 - AUGGGCAUUGACGACUU UG 19 15855

HSV2-UL48-317 - CAUGGGCAU UGACGACUUUG 20 5799

HSV2-U L48-1897 - CCAUGGGCAUUGACGACUU UG 21 15856

HSV2-U L48-1898 - UGCCAUGGGCAUUGACGACU UUG 23 15857

HSV2-UL48-1899 - AUGCCAUGGGCAUUGACGACU UUG 24 15858

HSV2-U L48-1900 - AGGGCAACCAGGCCCGCUCCU 21 15859

HSV2-UL48-1901 - CAGGGCAACCAGGCCCGCUCCU 22 15860

HSV2-UL48-1902 - UGCAGGGCAACCAGGCCCGCUCCU 24 15861

HSV2-UL48-849 - CGGGCGGACCAGAAACAAUU 20 6239

HSV2-UL48-1903 - CGCGGGCGGACCAGAAACAAUU 22 15862

HSV2-U L48-1904 - CCGCGGGCGGACCAGAAACAAUU 23 15863

HSV2-U L48-1905 - CUUUGGACGUGGACGAU U 18 15864

HSV2-UL48-1906 - UGGGGCUUUGGACGUGGACGAU U 23 15865

HSV2-UL48-1907 - AUGGGGCUUUGGACGUGGACGAU U 24

15866

Table 12G provides exemplary targeting domains for knocking out the UL48 gene selected according to the seven tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase). Table 12G

HSV2-UL48-1942 + CGCAGCAGAGGCCGUCGAACAGA 23 15901

HSV2-UL48-1943 + UCGCAGCAGAGGCCGUCGAACAGA 24 15902

HSV2-UL48-1944 + AGGAUCUCGCGGCUCAGA 18 15903

HSV2-UL48-1945 + UAGGAUCUCGCGGCUCAGA 19 15904

HSV2-UL48-983 + AU AGGAUCUCGCGGCUCAGA 20 6325

HSV2-UL48-1946 + CAU AGGAUCUCGCGGCUCAGA 21 15905

HSV2-UL48-1947 + CCAUAGGAUCUCGCGGCUCAGA 22 15906

HSV2-UL48-1948 + CCCAUAGGAUCUCGCGGCUCAGA 23 15907

HSV2-UL48-1949 + GCCCAUAGGAUCUCGCGGCUCAGA 24 15908

HSV2-UL48-1950 + CAUAAAGUACCCAGAGGA 18 15909

HSV2-UL48-1951 + GCAUAAAGUACCCAGAGGA 19 15910

HSV2-UL48-1009 + AGCAUAAAGUACCCAGAGGA 20 6344

HSV2-UL48-1952 + C AG C A U A A AG U ACCC AG AG G A 21 15911

HSV2-UL48-1953 + GCAGCAUAAAGUACCCAGAGGA 22 15912

HSV2-UL48-1954 + AGCAGCAUAAAGUACCCAGAGGA 23 15913

HSV2-UL48-1955 + CAG C AG C A U A A AG U ACCC AG AG G A 24 15914

HSV2-UL48-1956 + GGGGCGUUGUCCCGCGGA 18 15915

HSV2-UL48-1957 + GGGGGCGUUGUCCCGCGGA 19 15916

HSV2-UL48-1025 + CGGGGGCGUUGUCCCGCGGA 20 6355

HSV2-UL48-1958 + GCGGGGGCGU UGUCCCGCGGA 21 15917

HSV2-UL48-1959 + UGCGGGGGCG UUGUCCCGCGGA 22 15918

HSV2-UL48-1960 + CUGCGGGGGCGUUGUCCCGCGGA 23 15919

HSV2-UL48-1961 + UCUGCGGGGGCGU UGUCCCGCGGA 24 15920

HSV2-UL48-1962 + GUGGGUCAUUCCCGGGGA 18 15921

HSV2-UL48-1963 + CGUGGGUCAUUCCCGGGGA 19 15922

HSV2-UL48-331 + UCGUGGGUCAUUCCCGGGGA 20 5813

HSV2-UL48-1964 + GUCGUGGGUCAUUCCCGGGGA 21 15923

HSV2-UL48-1965 + GG UCGUGGGUCAU UCCCGGGGA 22 15924

HSV2-UL48-1966 + GGGUCGUGGGUCAUUCCCGGGGA 23 15925

HSV2-UL48-1967 + GGGGUCGUGGGUCAUUCCCGGGGA 24

15926

HSV2-UL48-1968 + GGCGCGCAGGUACCGGUA 18 15927

HSV2-UL48-1969 + UGGCGCGCAGGUACCGGUA 19 15928

HSV2-UL48-979 + CUGGCGCGCAGGUACCGGUA 20 6321

HSV2-UL48-1970 + GCUGGCGCGCAGGUACCGGUA 21 15929

HSV2-UL48-1971 + CGCUGGCGCGCAGGUACCGGUA 22 15930

HSV2-UL48-1972 + ACGCUGGCGCGCAGGUACCGGUA 23 15931

HSV2-UL48-1973 + AACGCUGGCGCGCAGGUACCGGUA 24 15932

HSV2-UL48-1974 + CCCAGGCUGACGUCGGUA 18 15933

HSV2-UL48-1975 + UCCCAGGCUGACGUCGGUA 19 15934

HSV2-UL48-1035 + CUCCCAGGCUGACGUCGGUA 20 6360

HSV2-UL48-1976 + UCUCCCAGGCUGACGUCGGUA 21 15935

HSV2-UL48-1977 + GUCUCCCAGGCUGACGUCGGUA 22 15936

HSV2-UL48-1978 + CGUCUCCCAGGCUGACGUCGGUA 23 15937

HSV2-UL48-1979 + UCGUCUCCCAGGCUGACGUCGGUA 24 15938

HSV2-UL48-1980 + CAGUCGCCGGGCCUCCAC 18 15939 HSV2-UL48-1981 + GCAG UCGCCGGGCCUCCAC 19 15940

HSV2-UL48-392 + CGCAGUCGCCGGGCCUCCAC 20 5874

HSV2-UL48-1982 + CCGCAGUCGCCGGGCCUCCAC 21 15941

HSV2-UL48-1983 + CCCGCAGUCGCCGGGCCUCCAC 22 15942

HSV2-UL48-1984 + UCCCGCAGUCGCCGGGCCUCCAC 23 15943

HSV2-UL48-1985 + CUCCCGCAGUCGCCGGGCCUCCAC 24 15944

HSV2-UL48-1986 + UCAGCAGCAUAAAGUACC 18 15945

HSV2-UL48-1987 + AUCAGCAGCAUAAAGUACC 19 15946

HSV2-UL48-1012 + G A U C AG C AG C A U A A AG U ACC 20 6346

HSV2-UL48-1988 + GGAUCAGCAGCAUAAAG UACC 21 15947

HSV2-UL48-1989 + CGG AUCAGCAGCAUAAAGUACC 22 15948

HSV2-UL48-1990 + CCG G A U C AG C AG C A U A A AG U ACC 23 15949

HSV2-UL48-1991 + CCCGGAUCAGCAGCAUAAAGUACC 24 15950

HSV2-UL48-1992 + CUGCGGGGGCGUUGUCCC 18 15951

HSV2-UL48-1993 + UCUGCGGGGGCGU UGUCCC 19 15952

HSV2-UL48-1028 + GUCUGCGGGGGCGU UGUCCC 20 6357

HSV2-UL48-1994 + AGUCUGCGGGGGCGUUGUCCC 21 15953

HSV2-UL48-1995 + CAGUCUGCGGGGGCGU UGUCCC 22 15954

HSV2-UL48-1996 + ACAGUCUGCGGGGGCGU UGUCCC 23 15955

HSV2-UL48-1997 + GACAGUCUGCGGGGGCGUUGUCCC 24 15956

HSV2-UL48-1998 + GGGUCGUGGGUCAUUCCC 18 15957

HSV2-UL48-1999 + GGGGUCGUGGGUCAUUCCC 19 15958

HSV2-UL48-328 + CGGGGUCGUGGGUCAUUCCC 20 5810

HSV2-UL48-2000 + ACGGGGUCGUGGGUCAUUCCC 21 15959

HSV2-UL48-2001 + GACGGGGUCGUGGGUCAUUCCC 22 15960

HSV2-UL48-2002 + AGACGGGGUCGUGGGUCAU UCCC 23 15961

HSV2-UL48-2003 + GAGACGGGGUCGUGGGUCAUUCCC 24 15962

HSV2-UL48-2004 + CGCAGUCGCCGGGCCUCC 18 15963

HSV2-UL48-2005 + CCGCAGUCGCCGGGCCUCC 19 15964

HSV2-UL48-995 + CCCGCAGUCGCCGGGCCUCC 20 6334

HSV2-UL48-2006 + UCCCGCAGUCGCCGGGCCUCC 21 15965

HSV2-UL48-2007 + CUCCCGCAGUCGCCGGGCCUCC 22 15966

HSV2-UL48-2008 + GCUCCCGCAGUCGCCGGGCCUCC 23 15967

HSV2-UL48-2009 + AGCUCCCGCAGUCGCCGGGCCUCC 24 15968

HSV2-UL48-2010 + GGAGCGUCAUCGUCGUCC 18 15969

HSV2-UL48-2011 + AGGAGCGUCAUCGUCGUCC 19 15970

HSV2-UL48-359 + CAGGAGCGUCAUCGUCGUCC 20 5841

HSV2-UL48-2012 + GCAGGAGCGUCAUCGUCGUCC 21 15971

HSV2-UL48-2013 + CGCAGGAGCGUCAUCGUCGUCC 22 15972

HSV2-UL48-2014 + CCGCAGGAGCGUCAUCG UCGUCC 23 15973

HSV2-UL48-2015 + UCCGCAGGAGCGUCAUCGUCGUCC 24 15974

HSV2-UL48-2016 + GGGGUCGUGGGUCAUUCC 18 15975

HSV2-UL48-2017 + CGGGGUCGUGGGUCAUUCC 19 15976

HSV2-UL48-327 + ACGGGGUCGUGGGUCAUUCC 20 5809

HSV2-UL48-2018 + GACGGGGUCGUGGGUCAUUCC 21 15977

HSV2-UL48-2019 + AGACGGGGUCGUGGGUCAU UCC 22 15978 HSV2-UL48-2020 + GAGACGGGGUCGUGGG UCAUUCC 23 15979

HSV2-UL48-2021 + CGAGACGGGGUCGUGGGUCAUUCC 24 15980

HSV2-UL48-2022 + GCCGCACUUCGCACCAGC 18 15981

HSV2-UL48-2023 + CGCCGCACUUCGCACCAGC 19 15982

HSV2-UL48-385 + CCGCCGCACU UCGCACCAGC 20 5867

HSV2-UL48-2024 + GCCGCCGCACUUCGCACCAGC 21 15983

HSV2-UL48-2025 + CGCCGCCGCACUUCGCACCAGC 22 15984

HSV2-UL48-2026 + CCGCCGCCGCACU UCGCACCAGC 23 15985

HSV2-UL48-2027 + UCCGCCGCCGCACUUCGCACCAGC 24 15986

HSV2-UL48-2028 + CACCAGCCCGGCCUCCGC 18 15987

HSV2-UL48-2029 + CCACCAGCCCGGCCUCCGC 19 15988

HSV2-UL48-357 + GCCACCAGCCCGGCCUCCGC 20 5839

HSV2-UL48-2030 + CGCCACCAGCCCGGCCUCCGC 21 15989

HSV2-UL48-2031 + GCGCCACCAGCCCGGCCUCCGC 22 15990

HSV2-UL48-2032 + GGCGCCACCAGCCCGGCCUCCGC 23 15991

HSV2-UL48-2033 + CGGCGCCACCAGCCCGGCCUCCGC 24 15992

HSV2-UL48-2034 + CGG UGGUGGACAGUCUGC 18 15993

HSV2-UL48-2035 + GCGGUGGUGGACAGUCUGC 19 15994

HSV2-UL48-351 + GGCGGUGGUGGACAGUCUGC 20 5833

HSV2-UL48-2036 + GGGCGGUGGUGGACAGUCUGC 21 15995

HSV2-UL48-2037 + GGGGCGGUGGUGGACAGUCUGC 22 15996

HSV2-UL48-2038 + GGGGGCGGUGGUGGACAGUCUGC 23 15997

HSV2-UL48-2039 + UGGGGGCGGUGG UGGACAGUCUGC 24

15998

HSV2-UL48-2040 + GAUCCGUUGAUAAACAUC 18 15999

HSV2-UL48-2041 + CGAUCCGU UGAUAAACAUC 19 16000

HSV2-UL48-394 + GCGAUCCGU UGAUAAACAUC 20 5876

HSV2-UL48-2042 + AGCGAUCCGUUGAUAAACAUC 21 16001

HSV2-UL48-2043 + GAGCGAUCCGU UGAUAAACAUC 22 16002

HSV2-UL48-2044 + UGAGCGAUCCGUUGAUAAACAUC 23 16003

HSV2-UL48-2045 + GUGAGCGAUCCGU UGAUAAACAUC 24 16004

HSV2-UL48-2046 + CUCCAAGUCGAAGUCGUC 18 16005

HSV2-UL48-2047 + UCUCCAAGUCGAAGUCGUC 19 16006

HSV2-UL48-1039 + AUCUCCAAGUCGAAGUCGUC 20 6363

HSV2-UL48-2048 + CAUCUCCAAGUCGAAGUCGUC 21 16007

HSV2-UL48-2049 + GCAUCUCCAAG UCGAAGUCGUC 22 16008

HSV2-UL48-2050 + AG C A U C U CCA AG UCGAAGUCGUC 23 16009

HSV2-UL48-2051 + CAGCAUCUCCAAGUCGAAGUCGUC 24 16010

HSV2-UL48-2052 + AGGAGCGUCAUCGUCGUC 18 16011

HSV2-UL48-2053 + CAGGAGCGUCAUCGUCGUC 19 16012

HSV2-UL48-358 + GCAGGAGCGUCAUCGUCGUC 20 5840

HSV2-UL48-2054 + CGCAGGAGCGUCAUCGUCGUC 21 16013

HSV2-UL48-2055 + CCGCAGGAGCG UCAUCGUCGUC 22 16014

HSV2-UL48-2056 + UCCGCAGGAGCGUCAUCGUCGUC 23 16015

HSV2-UL48-2057 + CUCCGCAGGAGCGUCAUCGUCGUC 24 16016

HSV2-UL48-2058 + UGCAGGACGGGCGGGGUC 18 16017 HSV2-UL48-2059 + CUGCAGGACGGGCGGGGUC 19 16018

HSV2-UL48-1004 + CCUGCAGGACGGGCGGGGUC 20 6340

HSV2-UL48-2060 + CCCUGCAGGACGGGCGGGGUC 21 16019

HSV2-UL48-2061 + GCCCUGCAGGACGGGCGGGGUC 22 16020

HSV2-UL48-2062 + UGCCCUGCAGGACGGGCGGGGUC 23 16021

HSV2-UL48-2063 + UUGCCCUGCAGGACGGGCGGGGUC 24 16022

HSV2-UL48-2064 + CGGGGUCGUGGGUCAU UC 18 16023

HSV2-UL48-2065 + ACGGGGUCGUGGGUCAUUC 19 16024

HSV2-UL48-1048 + GACGGGGUCGUGGGUCAUUC 20 6366

HSV2-UL48-2066 + AGACGGGGUCGUGGGUCAU UC 21 16025

HSV2-UL48-2067 + GAGACGGGGUCGUGGGUCAUUC 22 16026

HSV2-UL48-2068 + CGAGACGGGGUCGUGGGUCAUUC 23 16027

HSV2-UL48-2069 + ACGAGACGGGGUCGUGGGUCAUUC 24 16028

HSV2-UL48-2070 + UCCCCCAGCAUCUCCAAG 18 16029

HSV2-UL48-2071 + GUCCCCCAGCAUCUCCAAG 19 16030

HSV2-UL48-1040 + CG UCCCCCAGCAU CU CCAAG 20 6364

HSV2-UL48-2072 + ACG U CCCCCAGCAU CUCCAAG 21 16031

HSV2-UL48-2073 + CACGUCCCCCAGCAUCU CCAAG 22 16032

HSV2-UL48-2074 + CCACG U CCCCCAGCAU CU CCAAG 23 16033

HSV2-UL48-2075 + UCCACGUCCCCCAGCAUCUCCAAG 24 16034

HSV2-UL48-2076 + CGCCGCACUUCGCACCAG 18 16035

HSV2-UL48-2077 + CCGCCGCACUUCGCACCAG 19 16036

HSV2-UL48-384 + GCCGCCGCACUUCGCACCAG 20 5866

HSV2-UL48-2078 + CGCCGCCGCACUUCGCACCAG 21 16037

HSV2-UL48-2079 + CCGCCGCCGCACUUCGCACCAG 22 16038

HSV2-UL48-2080 + UCCGCCGCCGCACUUCGCACCAG 23 16039

HSV2-UL48-2081 + CUCCGCCGCCGCACU UCGCACCAG 24 16040

HSV2-UL48-2082 + GCAGCAUAAAGUACCCAG 18 16041

HSV2-UL48-2083 + AG C AG C A U A A AG U ACCC AG 19 16042

HSV2-UL48-369 + CAGCAGCAUAAAGUACCCAG 20 5851

HSV2-UL48-2084 + U C AG C AG C A U A A AG U ACCC AG 21 16043

HSV2-UL48-2085 + AUCAGCAGCAUAAAGUACCCAG 22 16044

HSV2-UL48-2086 + GAUCAGCAGCAUAAAGUACCCAG 23 16045

HSV2-UL48-2087 + GGAUCAGCAGCAUAAAG UACCCAG 24 16046

HSV2-UL48-2088 + CACCUCCUCGCCGUCCAG 18 16047

HSV2-UL48-2089 + CCACCUCCUCGCCGUCCAG 19 16048

HSV2-UL48-1037 + UCCACCUCCUCGCCGUCCAG 20 6361

HSV2-UL48-2090 + AUCCACCUCCUCGCCGUCCAG 21 16049

HSV2-UL48-2091 + UAUCCACCUCCUCGCCGUCCAG 22 16050

HSV2-UL48-2092 + AU AU CCACCU CCU CGCCG UCCAG 23 16051

HSV2-UL48-2093 + CAUAUCCACCUCCUCGCCGUCCAG 24 16052

HSV2-UL48-2094 + CAGCUCUCCAGGUCGCAG 18 16053

HSV2-UL48-2095 + CCAGCUCUCCAGGUCGCAG 19 16054

HSV2-UL48-988 + GCCAGCUCUCCAGGUCGCAG 20 6330

HSV2-UL48-2096 + CGCCAGCUCUCCAGGUCGCAG 21 16055

HSV2-UL48-2097 + GCGCCAGCUCUCCAGGUCGCAG 22 16056 HSV2-UL48-2098 + GGCGCCAGCUCUCCAGGUCGCAG 23 16057

HSV2-UL48-2099 + UGGCGCCAGCUCUCCAGGUCGCAG 24 16058

HSV2-UL48-2100 + UCCAAAGCCCCAUACGAG 18 16059

HSV2-UL48-2101 + GUCCAAAGCCCCAUACGAG 19 16060

HSV2-UL48-1051 + CGUCCAAAGCCCCAUACGAG 20 6368

HSV2-UL48-2102 + ACG U CCAAAGCCCCAU ACG AG 21 16061

HSV2-UL48-2103 + CACGU CCA A AG CCCCAUACGAG 22 16062

HSV2-UL48-2104 + CC ACG U CCA A AG CCCCAUACGAG 23 16063

HSV2-UL48-2105 + UCCACGUCCAAAGCCCCAUACGAG 24 16064

HSV2-UL48-2106 + UGGGUCAUUCCCGGGGAG 18 16065

HSV2-UL48-2107 + GUGGGUCAU UCCCGGGGAG 19 16066

HSV2-UL48-332 + CG UGGGUCAU UCCCGGGGAG 20 5814

HSV2-UL48-2108 + UCG UGGGUCAUUCCCGGGGAG 21 16067

HSV2-UL48-2109 + GUCGUGGGUCAUUCCCGGGGAG 22 16068

HSV2-UL48-2110 + GGUCGUGGGUCAUUCCCGGGGAG 23 16069

HSV2-UL48-2111 + GGGUCGUGGGUCAUUCCCGGGGAG 24

16070

HSV2-UL48-2112 + ACGGGAACUCCCCGCACG 18 16071

HSV2-UL48-2113 + CACGGGAACUCCCCGCACG 19 16072

HSV2-UL48-992 + CCACGGGAACUCCCCGCACG 20 6333

HSV2-UL48-2114 + UCCACGGGAACUCCCCGCACG 21 16073

HSV2-UL48-2115 + CUCCACGGGAACUCCCCGCACG 22 16074

HSV2-UL48-2116 + CCUCCACGGGAACUCCCCGCACG 23 16075

HSV2-UL48-2117 + GCCUCCACGGGAACUCCCCGCACG 24 16076

HSV2-UL48-2118 + CCCUCCGAGGUCGCGACG 18 16077

HSV2-UL48-2119 + GCCCUCCGAGG UCGCGACG 19 16078

HSV2-UL48-1016 + CGCCCUCCGAGGUCGCGACG 20 6349

HSV2-UL48-2120 + UCGCCCUCCGAGGUCGCGACG 21 16079

HSV2-UL48-2121 + CUCGCCCUCCGAGG UCGCGACG 22 16080

HSV2-UL48-2122 + ACUCGCCCUCCGAGGUCGCGACG 23 16081

HSV2-UL48-2123 + GACUCGCCCUCCGAGGUCGCGACG 24 16082

HSV2-UL48-2124 + UGCGGGGGCGUUGUCCCG 18 16083

HSV2-UL48-2125 + CUGCGGGGGCGUUGUCCCG 19 16084

HSV2-UL48-354 + UCUGCGGGGGCGUUGUCCCG 20 5836

HSV2-UL48-2126 + GUCUGCGGGGGCGU UGUCCCG 21 16085

HSV2-UL48-2127 + AGUCUGCGGGGGCGUUGUCCCG 22 16086

HSV2-UL48-2128 + CAGUCUGCGGGGGCGUUGUCCCG 23 16087

HSV2-UL48-2129 + ACAGUCUGCGGGGGCGU UGUCCCG 24 16088

HSV2-UL48-2130 + GGUCGUGGGUCAUUCCCG 18 16089

HSV2-UL48-2131 + GGGUCGUGGGUCAUUCCCG 19 16090

HSV2-UL48-329 + GGGGUCGUGGGUCAUUCCCG 20 5811

HSV2-UL48-2132 + CGGGGUCGUGGGUCAU UCCCG 21 16091

HSV2-UL48-2133 + ACGGGGUCGUGGGUCAU UCCCG 22 16092

HSV2-UL48-2134 + GACGGGGUCGUGGGUCAUUCCCG 23 16093

HSV2-UL48-2135 + AGACGGGGUCGUGGGUCAU UCCCG 24 16094

HSV2-UL48-2136 + AGGUCGCAGCAGAGGCCG 18 16095 HSV2-UL48-2137 + CAGG U CG C AG CAG AG G CCG 19 16096

HSV2-UL48-987 + CCAGGUCGCAGCAGAGGCCG 20 6329

HSV2-UL48-2138 + UCCAGGUCGCAGCAGAGGCCG 21 16097

HSV2-UL48-2139 + CUCCAGGUCGCAGCAGAGGCCG 22 16098

HSV2-UL48-2140 + UCUCCAGGUCGCAGCAGAGGCCG 23 16099

HSV2-UL48-2141 + CUCUCCAGGUCGCAGCAGAGGCCG 24 16100

HSV2-UL48-2142 + CCACCAGCCCGG CCU CCG 18 16101

HSV2-UL48-2143 + GCCACCAGCCCGGCCUCCG 19 16102

HSV2-UL48-1024 + CGCCACCAGCCCGGCCUCCG 20 6354

HSV2-UL48-2144 + GCGCCACCAGCCCGGCCUCCG 21 16103

HSV2-UL48-2145 + GGCGCCACCAGCCCGGCCUCCG 22 16104

HSV2-UL48-2146 + CGGCGCCACCAGCCCGGCCUCCG 23 16105

HSV2-UL48-2147 + GCGGCGCCACCAGCCCGGCCUCCG 24 16106

HSV2-UL48-2148 + CGGGGGCGUUGUCCCGCG 18 16107

HSV2-UL48-2149 + GCGGGGGCGUUGUCCCGCG 19 16108

HSV2-UL48-1026 + UGCGGGGGCGUUGUCCCGCG 20 6356

HSV2-UL48-2150 + CUGCGGGGGCGUUGUCCCGCG 21 16109

HSV2-UL48-2151 + UCUGCGGGGGCGUUGUCCCGCG 22 16110

HSV2-UL48-2152 + GUCUGCGGGGGCGUUG UCCCGCG 23 16111

HSV2-UL48-2153 + AGUCUGCGGGGGCGUUGUCCCGCG 24 16112

HSV2-UL48-2154 + GUUUAGCUCCCGCAGUCG 18 16113

HSV2-UL48-2155 + GGUUUAGCUCCCGCAGUCG 19 16114

HSV2-UL48-996 + UGGU UUAGCUCCCGCAGUCG 20 6335

HSV2-UL48-2156 + GUGGU UUAGCUCCCGCAGUCG 21 16115

HSV2-UL48-2157 + UGUGGUUUAGCUCCCGCAGUCG 22 16116

HSV2-UL48-2158 + AUGUGGUUUAGCUCCCGCAG UCG 23 16117

HSV2-UL48-2159 + AAUGUGGUU UAGCUCCCGCAGUCG 24 16118

HSV2-UL48-2160 + AAGUCGUCCAGGGCGUCG 18 16119

HSV2-UL48-2161 + GAAGUCG UCCAGGGCGUCG 19 16120

HSV2-UL48-1038 + CGAAGUCGUCCAGGGCGUCG 20 6362

HSV2-UL48-2162 + UCGAAGUCGUCCAGGGCGUCG 21 16121

HSV2-UL48-2163 + GUCGAAGUCGUCCAGGGCGUCG 22 16122

HSV2-UL48-2164 + AGUCGAAGUCGUCCAGGGCGUCG 23 16123

HSV2-UL48-2165 + AAGUCGAAGUCGUCCAGGGCGUCG 24 16124

HSV2-UL48-2166 + GCCUGGUUGCCCUGCAGG 18 16125

HSV2-UL48-2167 + GGCCUGGU UGCCCUGCAGG 19 16126

HSV2-UL48-1007 + GGGCCUGGUUGCCCUGCAGG 20 6342

HSV2-UL48-2168 + CGGGCCUGGUUGCCCUGCAGG 21 16127

HSV2-UL48-2169 + GCGGGCCUGGUUGCCCUGCAGG 22 16128

HSV2-UL48-2170 + AGCGGGCCUGGUUGCCCUGCAGG 23 16129

HSV2-UL48-2171 + GAGCGGGCCUGGUUGCCCUGCAGG 24 16130

HSV2-UL48-2172 + UCAUCGUCG UCCGGGAGG 18 16131

HSV2-UL48-2173 + GUCAUCGUCGUCCGGGAGG 19 16132

HSV2-UL48-1019 + CGUCAUCGUCGUCCGGGAGG 20 6352

HSV2-UL48-2174 + GCG UCAUCGUCGUCCGGGAGG 21 16133

HSV2-UL48-2175 + AGCG UCAUCGUCGUCCGGGAGG 22 16134 HSV2-UL48-2176 + GAGCGUCAUCGUCGUCCGGGAGG 23 16135

HSV2-UL48-2177 + GGAGCGUCAUCGUCGUCCGGGAGG 24 16136

HSV2-UL48-2178 + GGUUGCCCUGCAGGACGG 18 16137

HSV2-UL48-2179 + UGGUUGCCCUGCAGGACGG 19 16138

HSV2-UL48-1006 + CUGGUUGCCCUGCAGGACGG 20 6341

HSV2-UL48-2180 + CCUGGUUGCCCUGCAGGACGG 21 16139

HSV2-UL48-2181 + GCCUGGUUGCCCUGCAGGACGG 22 16140

HSV2-UL48-2182 + GGCCUGGU UGCCCUGCAGGACGG 23 16141

HSV2-UL48-2183 + GGGCCUGGU UGCCCUGCAGGACGG 24 16142

HSV2-UL48-2184 + GCAGGUACCGGUACAGGG 18 16143

HSV2-UL48-2185 + CGCAGGUACCGGUACAGGG 19 16144

HSV2-UL48-978 + GCGCAGGUACCGGUACAGGG 20 6320

HSV2-UL48-2186 + CGCGCAGGUACCGGUACAGGG 21 16145

HSV2-UL48-2187 + GCGCGCAGGUACCGGUACAGGG 22 16146

HSV2-UL48-2188 + GGCGCGCAGG UACCGGUACAGGG 23 16147

HSV2-UL48-2189 + UGGCGCGCAGGUACCGGUACAGGG 24 16148

HSV2-UL48-2190 + UCGUGGGUCAUUCCCGGG 18 16149

HSV2-UL48-2191 + GUCGUGGGUCAUUCCCGGG 19 16150

HSV2-UL48-1044 + GGUCGUGGGUCAUUCCCGGG 20 6365

HSV2-UL48-2192 + GGG UCGUGGGUCAUUCCCGGG 21 16151

HSV2-UL48-2193 + GGGG UCGUGGGUCAUUCCCGGG 22 16152

HSV2-UL48-2194 + CGGGG UCGUGGGUCAUUCCCGGG 23 16153

HSV2-UL48-2195 + ACGGGGUCGUGGGUCAUUCCCGGG 24 16154

HSV2-UL48-2196 + GUUGAUAAACAUCAGGGG 18 16155

HSV2-UL48-2197 + CGU UGAUAAACAUCAGGGG 19 16156

HSV2-UL48-989 + CCGU UGAUAAACAUCAGGGG 20 6331

HSV2-UL48-2198 + UCCGUUGAUAAACAUCAGGGG 21 16157

HSV2-UL48-2199 + AUCCGUUGAUAAACAUCAGGGG 22 16158

HSV2-UL48-2200 + GAUCCGUUGAUAAACAUCAGGGG 23 16159

HSV2-UL48-2201 + CGAUCCGU UGAUAAACAUCAGGGG 24 16160

HSV2-UL48-2202 + CGUGGGUCAU UCCCGGGG 18 16161

HSV2-UL48-2203 + UCGUGGGUCAUUCCCGGGG 19 16162

HSV2-UL48-330 + GUCGUGGGUCAUUCCCGGGG 20 5812

HSV2-UL48-2204 + GG UCGUGGGUCAUUCCCGGGG 21 16163

HSV2-UL48-2205 + GGG UCGUGGGUCAUUCCCGGGG 22 16164

HSV2-UL48-2206 + GGGG UCGUGGGUCAUUCCCGGGG 23 16165

HSV2-UL48-2207 + CGGGGUCGUGGGUCAUUCCCGGGG 24 16166

HSV2-UL48-2208 + UACGCGUGCUCCCGCAUG 18 16167

HSV2-UL48-2209 + AUACGCGUGCUCCCGCAUG 19 16168

HSV2-UL48-1018 + UAUACGCGUGCUCCCGCAUG 20 6351

HSV2-UL48-2210 + CUAUACGCGUGCUCCCGCAUG 21 16169

HSV2-UL48-2211 + GCUAUACGCG UGCUCCCGCAUG 22 16170

HSV2-UL48-2212 + GGCUAUACGCGUGCUCCCGCAUG 23 16171

HSV2-UL48-2213 + CGGCUAUACGCGUGCUCCCGCAUG 24 16172

HSV2-UL48-2214 + CAGAAAGAGGUAUAGAUG 18 16173

HSV2-UL48-2215 + UCAGAAAGAGGUAUAGAUG 19 16174 HSV2-UL48-982 + CUCAGAAAGAGGUAUAGAUG 20 6324

HSV2-UL48-2216 + GCUCAGAAAGAGGUAUAGAUG 21 16175

HSV2-UL48-2217 + GGCUCAGAAAGAGGUAUAGAUG 22 16176

HSV2-UL48-2218 + CGGCUCAGAAAGAGGUAUAGAUG 23 16177

HSV2-UL48-2219 + GCGGCUCAGAAAGAGGUAUAGAUG 24 16178

HSV2-UL48-2220 + GCGGGCCUGGUUGCCCUG 18 16179

HSV2-UL48-2221 + AGCGGGCCUGGUUGCCCUG 19 16180

HSV2-UL48-1008 + GAGCGGGCCUGGUUGCCCUG 20 6343

HSV2-UL48-2222 + GGAGCGGGCCUGGUUGCCCUG 21 16181

HSV2-UL48-2223 + AGGAGCGGGCCUGGUUGCCCUG 22 16182

HSV2-UL48-2224 + GAGGAGCGGGCCUGGUUGCCCUG 23 16183

HSV2-UL48-2225 + AGAGGAGCGGGCCUGGU UGCCCUG 24 16184

HSV2-UL48-2226 + UGCCUGGCGGUG UAGCUG 18 16185

HSV2-UL48-2227 + GUGCCUGGCGGUGUAGCUG 19 16186

HSV2-UL48-980 + UGUGCCUGGCGG UGUAGCUG 20 6322

HSV2-UL48-2228 + GUGUGCCUGGCGGUGUAGCUG 21 16187

HSV2-UL48-2229 + UGUGUGCCUGGCGGUGUAGCUG 22 16188

HSV2-UL48-2230 + AUG UGUGCCUGGCGGUGUAGCUG 23 16189

HSV2-UL48-2231 + CAUGUG UGCCUGGCGGUGUAGCUG 24

16190

HSV2-UL48-2232 + GCGGUGGUGGACAG UCUG 18 16191

HSV2-UL48-2233 + GGCGGUGGUGGACAGUCUG 19 16192

HSV2-UL48-350 + GGGCGGUGGUGGACAGUCUG 20 5832

HSV2-UL48-2234 + GGGGCGGUGGUGGACAGUCUG 21 16193

HSV2-UL48-2235 + GGGGGCGGUGGUGGACAGUCUG 22 16194

HSV2-UL48-2236 + UGGGGGCGGUGGUGGACAGUCUG 23 16195

HSV2-UL48-2237 + AUGGGGGCGGUGGUGGACAGUCUG 24

16196

HSV2-UL48-2238 + AGGACGGGCGGGGUCG UG 18 16197

HSV2-UL48-2239 + CAGGACGGGCGGGGUCGUG 19 16198

HSV2-UL48-379 + GCAGGACGGGCGGGGUCGUG 20 5861

HSV2-UL48-2240 + UGCAGGACGGGCGGGGUCGUG 21 16199

HSV2-UL48-2241 + CUGCAGGACGGGCGGGGUCGUG 22 16200

HSV2-UL48-2242 + CCUGCAGGACGGGCGGGGUCGUG 23 16201

HSV2-UL48-2243 + CCCUGCAGGACGGGCGGGGUCGUG 24 16202

HSV2-UL48-2244 + UCGGUAAUGGGGGCGGUG 18 16203

HSV2-UL48-2245 + GUCGGUAAUGGGGGCGGUG 19 16204

HSV2-UL48-1032 + CGUCGGUAAUGGGGGCGGUG 20 6359

HSV2-UL48-2246 + ACG UCGGUAAUGGGGGCGGUG 21 16205

HSV2-UL48-2247 + GACG UCGGUAAUGGGGGCGGUG 22 16206

HSV2-UL48-2248 + UGACGUCGGUAAUGGGGGCGGUG 23 16207

HSV2-UL48-2249 + CUGACGUCGGUAAUGGGGGCGGUG 24

16208

HSV2-UL48-2250 + CAGGCUGACGUCGGUAAU 18 16209

HSV2-UL48-2251 + CCAGGCUGACGUCGGUAAU 19 16210

HSV2-UL48-344 + CCCAGGCUGACGUCGGUAAU 20 5826 HSV2-UL48-2252 + UCCCAGGCUGACGUCGGUAAU 21 16211

HSV2-UL48-2253 + CUCCCAGGCUGACGUCGGUAAU 22 16212

HSV2-UL48-2254 + UCUCCCAGGCUGACGUCGGUAAU 23 16213

HSV2-UL48-2255 + GUCUCCCAGGCUGACGUCGGUAAU 24 16214

HSV2-UL48-2256 + CGAUCCGUUGAUAAACAU 18 16215

HSV2-UL48-2257 + GCGAUCCGU UGAUAAACAU 19 16216

HSV2-UL48-991 + AGCGAUCCGU UGAUAAACAU 20 6332

HSV2-UL48-2258 + GAGCGAUCCGU UGAUAAACAU 21 16217

HSV2-UL48-2259 + UGAGCGAUCCGUUGAUAAACAU 22 16218

HSV2-UL48-2260 + GUG AGCGAUCCGUUGAUAAACAU 23 16219

HSV2-UL48-2261 + GGUGAGCGAUCCGUUGAUAAACAU 24 16220

HSV2-UL48-2262 + CCACG UCCAAAG CCCCAU 18 16221

HSV2-UL48-2263 + UCCACGUCCAAAGCCCCAU 19 16222

HSV2-UL48-1052 + G U CCACG U CCAAAGCCCCAU 20 6369

HSV2-UL48-2264 + CGUCCACGU CCA A AG CCCCAU 21 16223

HSV2-UL48-2265 + U CG U CCACG U CCAAAG CCCCAU 22 16224

HSV2-UL48-2266 + AUCGUCCACGU CCAAAG CCCCAU 23 16225

HSV2-UL48-2267 + A A U CG U CC ACG U CC A A AG CCCC A U 24 16226

HSV2-UL48-2268 + GCAUGACGGACUCGCCCU 18 16227

HSV2-UL48-2269 + CGCAUGACGGACUCGCCCU 19 16228

HSV2-UL48-1017 + CCGCAUGACGGACUCGCCCU 20 6350

HSV2-UL48-2270 + CCCGCAUGACGGACUCGCCCU 21 16229

HSV2-UL48-2271 + UCCCGCAUGACGGACUCGCCCU 22 16230

HSV2-UL48-2272 + CUCCCGCAUGACGGACUCGCCCU 23 16231

HSV2-UL48-2273 + GCUCCCGCAUGACGGACUCGCCCU 24 16232

HSV2-UL48-2274 + CCAUAGGAUCUCGCGGCU 18 16233

HSV2-UL48-2275 + CCCAUAGGAUCUCGCGGCU 19 16234

HSV2-UL48-984 + GCCCAUAGGAUCUCGCGGCU 20 6326

HSV2-UL48-2276 + GGCCCAUAGGAUCUCGCGGCU 21 16235

HSV2-UL48-2277 + CGGCCCAUAGGAUCUCGCGGCU 22 16236

HSV2-UL48-2278 + GCGGCCCAUAGGAUCUCGCGGCU 23 16237

HSV2-UL48-2279 + CGCGGCCCAUAGGAUCUCGCGGCU 24 16238

HSV2-UL48-2280 + GGCGGUGGUGGACAGUCU 18 16239

HSV2-UL48-2281 + GGGCGGUGGUGGACAGUCU 19 16240

HSV2-UL48-1031 + GGGGCGGUGGUGGACAGUCU 20 6358

HSV2-UL48-2282 + GGGGGCGGUGGUGGACAGUCU 21 16241

HSV2-UL48-2283 + UGGGGGCGGUGGUGGACAGUCU 22 16242

HSV2-UL48-2284 + AUGGGGGCGGUGG UGGACAGUCU 23 16243

HSV2-UL48-2285 + AAUGGGGGCGGUGGUGGACAGUCU 24

16244

HSV2-UL48-2286 + CAGGAGCGUCAUCGUCGU 18 16245

HSV2-UL48-2287 + GCAGGAGCGUCAUCGUCGU 19 16246

HSV2-UL48-1022 + CGCAGGAGCGUCAUCGUCGU 20 6353

HSV2-UL48-2288 + CCGCAGGAGCGUCAUCGUCGU 21 16247

HSV2-UL48-2289 + UCCGCAGGAGCGUCAUCGUCGU 22 16248

HSV2-UL48-2290 + CUCCGCAGGAGCGUCAUCGUCGU 23 16249 HSV2-UL48-2291 + CCUCCGCAGGAGCGUCAUCGUCGU 24 16250

HSV2-UL48-2292 + CAGGACGGGCGGGGUCGU 18 16251

HSV2-UL48-2293 + GCAGGACGGGCGGGGUCGU 19 16252

HSV2-UL48-1003 + UGCAGGACGGGCGGGGUCGU 20 6339

HSV2-UL48-2294 + CUGCAGGACGGGCGGGGUCGU 21 16253

HSV2-UL48-2295 + CCUGCAGGACGGGCGGGGUCGU 22 16254

HSV2-UL48-2296 + CCCUGCAGGACGGGCGGGGUCGU 23 16255

HSV2-UL48-2297 + GCCCUGCAGGACGGGCGGGGUCGU 24 16256

HSV2-UL48-2298 - ACAAUUACGGAUCGACAA 18 16257

HSV2-UL48-2299 - AACAAU U ACGG AUCG ACAA 19 16258

HSV2-UL48-850 - A A AC A A UUACGGAU CG AC A A 20 6240

HSV2-UL48-2300 - GAAACAAUU ACGG AUCG ACAA 21 16259

HSV2-UL48-2301 - AG AAACAAUU ACGG AUCG ACAA 22 16260

HSV2-UL48-2302 - CAGAAACAAUUACGGAUCGACAA 23 16261

HSV2-UL48-2303 - CCAGAAACAAUU ACGG AUCG ACAA 24 16262

HSV2-UL48-2304 - GGGGGGUAGGAUGUGCGA 18 16263

HSV2-UL48-2305 - UGGGGGGUAGGAUGUGCGA 19 16264

HSV2-UL48-2306 - UUGGGGGGUAGGAUGUGCGA 20 16265

HSV2-UL48-2307 - U U UGGGGGGUAGGAUGUGCGA 21 16266

HSV2-UL48-2308 - CU U UGGGGGG UAGGAUGUGCGA 22 16267

HSV2-UL48-2309 - ACU UUGGGGGGUAGGAUGUGCGA 23 16268

HSV2-UL48-2310 - GACUUUGGGGGGUAGGAUGUGCGA 24

16269

HSV2-UL48-1612 - GGCGCGGGAGGAGAGCUA 18 15571

HSV2-UL48-1613 - GGGCGCGGGAGGAGAGCUA 19 15572

HSV2-UL48-651 - CGGGCGCGGGAGGAGAGCUA 20 6120

HSV2-UL48-1614 - GCGGGCGCGGGAGGAGAGCUA 21 15573

HSV2-UL48-1615 - UGCGGGCGCGGGAGGAGAGCUA 22 15574

HSV2-UL48-1616 - CUGCGGGCGCGGGAGGAGAGCUA 23 15575

HSV2-UL48-1617 - GCUGCGGGCGCGGGAGGAGAGCUA 24 15576

HSV2-UL48-2311 - CCACGACCCCGUCUCGUA 18 16270

HSV2-UL48-2312 - CCCACG ACCCCG U CU CG U A 19 16271

HSV2-UL48-308 - ACCCACG ACCCCG UCUCGUA 20 5790

HSV2-UL48-2313 - GACCCACGACCCCGUCUCGUA 21 16272

HSV2-UL48-2314 - UGACCCACGACCCCGUCUCGUA 22 16273

HSV2-UL48-2315 - AUG ACCCACG ACCCCG UCUCGUA 23 16274

HSV2-UL48-2316 - AAUG ACCCACG ACCCCG UCUCGUA 24 16275

HSV2-UL48-2317 - GUGCGGCGGCGGAGGAAC 18 16276

HSV2-UL48-2318 - AGUGCGGCGGCGGAGGAAC 19 16277

HSV2-UL48-833 - AAGUGCGGCGGCGGAGGAAC 20 6227

HSV2-UL48-2319 - GAAGUGCGGCGGCGGAGGAAC 21 16278

HSV2-UL48-2320 - CG AAGUGCGGCGGCGGAGGAAC 22 16279

HSV2-UL48-2321 - GCG AAGUGCGGCGGCGGAGGAAC 23 16280

HSV2-UL48-2322 - UGCGAAGUGCGGCGGCGGAGGAAC 24 16281

HSV2-UL48-2323 - A ACC AC A U U CG CG AG C AC 18 16282

HSV2-UL48-2324 - AAACCACAU UCGCGAGCAC 19 16283 HSV2-UL48-827 - UAAACCACAUUCGCGAGCAC 20 6223

HSV2-UL48-2325 - CUAAACCACAUUCGCGAGCAC 21 16284

HSV2-UL48-2326 - GCUAAACCACAUUCGCGAGCAC 22 16285

HSV2-UL48-2327 - AGCUAAACCACAUUCGCGAGCAC 23 16286

HSV2-UL48-2328 - G AG CU AAACC ACAU U CG CG AG C AC 24 16287

HSV2-UL48-2329 - GACGGCCUCUGCUGCGAC 18 16288

HSV2-UL48-2330 - CGACGGCCUCUGCUGCGAC 19 16289

HSV2-UL48-813 - UCGACGGCCUCUGCUGCGAC 20 6216

HSV2-UL48-2331 - UUCGACGGCCUCUGCUGCGAC 21 16290

HSV2-UL48-2332 - GUUCGACGGCCUCUGCUGCGAC 22 16291

HSV2-UL48-2333 - UGUUCGACGGCCUCUGCUGCGAC 23 16292

HSV2-UL48-2334 - CUGU UCGACGGCCUCUGCUGCGAC 24 16293

HSV2-UL48-2335 - GCCCUGGACGACUUCGAC 18 16294

HSV2-UL48-2336 - CGCCCUGGACGACUUCGAC 19 16295

HSV2-UL48-868 - ACGCCCUGGACGACUUCGAC 20 6253

HSV2-UL48-2337 - GACGCCCUGGACGACUUCGAC 21 16296

HSV2-UL48-2338 - CGACGCCCUGGACGACUUCGAC 22 16297

HSV2-UL48-2339 - CCGACGCCCUGGACGACUUCGAC 23 16298

HSV2-UL48-2340 - GCCG ACGCCCUGGACGACU UCGAC 24 16299

HSV2-UL48-2341 - GUAUAGCCGCGGGCGGAC 18 16300

HSV2-UL48-2342 - CGUAUAGCCGCGGGCGGAC 19 16301

HSV2-UL48-848 - GCGUAUAGCCGCGGGCGGAC 20 6238

HSV2-UL48-2343 - CGCGUAUAGCCGCGGGCGGAC 21 16302

HSV2-UL48-2344 - ACGCGUAUAGCCGCGGGCGGAC 22 16303

HSV2-UL48-2345 - CACGCGUAUAGCCGCGGGCGGAC 23 16304

HSV2-UL48-2346 - GCACGCGUAUAGCCGCGGGCGGAC 24 16305

HSV2-UL48-2347 - UUGGAGAUGCUGGGGGAC 18 16306

HSV2-UL48-2348 - CUUGGAGAUGCUGGGGGAC 19 16307

HSV2-UL48-874 - ACU UGGAGAUGCUGGGGGAC 20 6255

HSV2-UL48-2349 - GACU UGGAGAUGCUGGGGGAC 21 16308

HSV2-UL48-2350 - CG ACUUGGAGAUGCUGGGGGAC 22 16309

HSV2-UL48-2351 - UCG ACUUGGAGAUGCUGGGGGAC 23 16310

HSV2-UL48-2352 - UUCGACU UGGAGAUGCUGGGGGAC 24

16311

HSV2-UL48-2353 - UCGUAUGGGGCUUUGGAC 18 16312

HSV2-UL48-2354 - CUCGUAUGGGGCUUUGGAC 19 16313

HSV2-UL48-882 - UCUCGUAUGGGGCUUUGGAC 20 6259

HSV2-UL48-2355 - GUCUCGUAUGGGGCUU UGGAC 21 16314

HSV2-UL48-2356 - CGUCUCGUAUGGGGCUUUGGAC 22 16315

HSV2-UL48-2357 - CCGUCUCGUAUGGGGCUUUGGAC 23 16316

HSV2-UL48-2358 - CCCGUCUCGUAUGGGGCUUUGGAC 24 16317

HSV2-UL48-2359 - UGCGGCGGCGGAGGAACC 18 16318

HSV2-UL48-2360 - GUGCGGCGGCGGAGGAACC 19 16319

HSV2-UL48-261 - AG UGCGGCGGCGGAGGAACC 20 5743

HSV2-UL48-2361 - AAG UGCGGCGGCGGAGGAACC 21 16320

HSV2-UL48-2362 - GAAG UGCGGCGGCGGAGGAACC 22 16321 HSV2-UL48-2363 - CGAAGUGCGGCGGCGGAGGAACC 23 16322

HSV2-UL48-2364 - GCGAAGUGCGGCGGCGGAGGAACC 24 16323

HSV2-UL48-2365 - UACUCCAGCGUCGCGACC 18 16324

HSV2-UL48-2366 - GUACUCCAGCGUCGCGACC 19 16325

HSV2-UL48-839 - CGUACUCCAGCGUCGCGACC 20 6232

HSV2-UL48-2367 - UCGUACUCCAGCGUCGCGACC 21 16326

HSV2-UL48-2368 - CUCGUACUCCAGCGUCGCGACC 22 16327

HSV2-UL48-2369 - ACUCGUACUCCAGCGUCGCGACC 23 16328

HSV2-UL48-2370 - GACUCGUACUCCAGCGUCGCGACC 24 16329

HSV2-UL48-2371 - ACGGCCUCUGCUGCGACC 18 16330

HSV2-UL48-2372 - GACGGCCUCUGCUGCGACC 19 16331

HSV2-UL48-244 - CGACGGCCUCUGCUGCGACC 20 5726

HSV2-UL48-2373 - UCGACGGCCUCUGCUGCGACC 21 16332

HSV2-UL48-2374 - UUCGACGGCCUCUGCUGCGACC 22 16333

HSV2-UL48-2375 - GU UCGACGGCCUCUGCUGCGACC 23 16334

HSV2-UL48-2376 - UGUUCGACGGCCUCUGCUGCGACC 24 16335

HSV2-UL48-2377 - UCGCGGACAGGUACUACC 18 16336

HSV2-UL48-2378 - AUCGCGGACAGGUACUACC 19 16337

HSV2-UL48-808 - GAUCGCGGACAGGUACUACC 20 6211

HSV2-UL48-2379 - CG AUCGCGGACAGGUACUACC 21 16338

HSV2-UL48-2380 - ACG AUCGCGGACAGGUACUACC 22 16339

HSV2-UL48-2381 - CACG AUCGCGGACAGGUACUACC 23 16340

HSV2-UL48-2382 - CCACGAUCGCGGACAGGUACUACC 24 16341

HSV2-UL48-2383 - ACGUGGAGUCCCCCUCCC 18 16342

HSV2-UL48-2384 - GACGUGGAGUCCCCCUCCC 19 16343

HSV2-UL48-306 - GGACGUGGAGUCCCCCUCCC 20 5788

HSV2-UL48-2385 - GGGACGUGGAGUCCCCCUCCC 21 16344

HSV2-UL48-2386 - GGGGACGUGGAGUCCCCCUCCC 22 16345

HSV2-UL48-2387 - GGGGGACGUGGAGUCCCCCUCCC 23 16346

HSV2-UL48-2388 - UGGGGGACGUGGAGUCCCCCUCCC 24 16347

HSV2-UL48-2389 - ACCGUGCGGGGAGUUCCC 18 16348

HSV2-UL48-2390 - CACCGUGCGGGGAGUUCCC 19 16349

HSV2-UL48-821 - UCACCGUGCGGGGAGU UCCC 20 6220

HSV2-UL48-2391 - CUCACCGUGCGGGGAGU UCCC 21 16350

HSV2-UL48-2392 - GCUCACCGUGCGGGGAG UUCCC 22 16351

HSV2-UL48-2393 - CGCUCACCGUGCGGGGAGUUCCC 23 16352

HSV2-UL48-2394 - UCGCUCACCGUGCGGGGAGUUCCC 24 16353

HSV2-UL48-2395 - CCAUUACCGACGUCAGCC 18 16354

HSV2-UL48-2396 - CCCAU UACCGACGUCAGCC 19 16355

HSV2-UL48-292 - CCCCAUUACCGACGUCAGCC 20 5774

HSV2-UL48-2397 - CCCCCAUUACCGACGUCAGCC 21 16356

HSV2-UL48-2398 - GCCCCCAUUACCGACGUCAGCC 22 16357

HSV2-UL48-2399 - CGCCCCCAUUACCGACGUCAGCC 23 16358

HSV2-UL48-2400 - CCGCCCCCAU UACCGACGUCAGCC 24 16359

HSV2-UL48-2401 - UAUACCUCU UUCUGAGCC 18 16360

HSV2-UL48-2402 - CUAUACCUCU UUCUGAGCC 19 16361 HSV2-UL48-810 - UCUAUACCUCUUUCUGAGCC 20 6213

HSV2-UL48-2403 - AUCUAUACCUCUU UCUGAGCC 21 16362

HSV2-UL48-2404 - CAUCUAUACCUCUUUCUGAGCC 22 16363

HSV2-UL48-2405 - GCAUCUAUACCUCUUUCUGAGCC 23 16364

HSV2-UL48-2406 - UGCAUCUAUACCUCUUUCUGAGCC 24 16365

HSV2-UL48-2407 - GGGAGCACGCGUAUAGCC 18 16366

HSV2-UL48-2408 - CGGGAGCACGCGUAUAGCC 19 16367

HSV2-UL48-846 - GCGGGAGCACGCGUAUAGCC 20 6236

HSV2-UL48-2409 - UGCGGGAGCACGCGUAUAGCC 21 16368

HSV2-UL48-2410 - AUGCGGGAGCACGCGUAUAGCC 22 16369

HSV2-UL48-2411 - CAUGCGGGAGCACGCGUAUAGCC 23 16370

HSV2-UL48-2412 - UCAUGCGGGAGCACGCGUAUAGCC 24 16371

HSV2-UL48-2413 - AUGACGCCCGCCGACGCC 18 16372

HSV2-UL48-2414 - UAUGACGCCCGCCGACGCC 19 16373

HSV2-UL48-867 - AUAUGACGCCCGCCGACGCC 20 6252

HSV2-UL48-2415 - GAUAUGACGCCCGCCGACGCC 21 16374

HSV2-UL48-2416 - GGAUAUGACGCCCGCCGACGCC 22 16375

HSV2-UL48-2417 - UGGAUAUGACGCCCGCCGACGCC 23 16376

HSV2-UL48-2418 - GUGGAUAUGACGCCCGCCGACGCC 24 16377

HSV2-UL48-2419 - CAGAUGU UUACCGAUGCC 18 16378

HSV2-UL48-2420 - ACAGAUGUU UACCGAUGCC 19 16379

HSV2-UL48-885 - AACAGAUGU UUACCGAUGCC 20 6262

HSV2-UL48-2421 - GAACAGAUGUUUACCGAUGCC 21 16380

HSV2-UL48-2422 - UGAACAGAUGUUUACCGAUGCC 22 16381

HSV2-UL48-2423 - UUGAACAGAUGUUUACCGAUGCC 23 16382

HSV2-UL48-2424 - UUUGAACAGAUGUUUACCGAUGCC 24 16383

HSV2-UL48-2425 - GACGUGGAGUCCCCCUCC 18 16384

HSV2-UL48-2426 - GGACGUGGAGUCCCCCUCC 19 16385

HSV2-UL48-876 - GGGACGUGGAGUCCCCCUCC 20 6256

HSV2-UL48-2427 - GGGGACGUGGAGUCCCCCUCC 21 16386

HSV2-UL48-2428 - GGGGGACGUGGAGUCCCCCUCC 22 16387

HSV2-UL48-2429 - UGGGGGACGUGGAGUCCCCCUCC 23 16388

HSV2-UL48-2430 - CUGGGGGACGUGGAGUCCCCCUCC 24 16389

HSV2-UL48-2431 - CGCAUGUCGU UUCUCUCC 18 16390

HSV2-UL48-2432 - GCGCAUGUCGU UUCUCUCC 19 16391

HSV2-UL48-856 - CGCGCAUGUCGUU UCUCUCC 20 6245

HSV2-UL48-2433 - CCGCGCAUGUCGU UUCUCUCC 21 16392

HSV2-UL48-2434 - GCCGCGCAUGUCGUUUCUCUCC 22 16393

HSV2-UL48-2435 - CGCCGCGCAUGUCGUU UCUCUCC 23 16394

HSV2-UL48-2436 - GCGCCGCGCAUGUCGU U UCUCUCC 24 16395

HSV2-UL48-2437 - CCCAUUACCGACGUCAGC 18 16396

HSV2-UL48-2438 - CCCCAUUACCGACGUCAGC 19 16397

HSV2-UL48-858 - CCCCCAUUACCGACGUCAGC 20 6246

HSV2-UL48-2439 - GCCCCCAUUACCGACGUCAGC 21 16398

HSV2-UL48-2440 - CGCCCCCAUUACCGACGUCAGC 22 16399

HSV2-UL48-2441 - CCGCCCCCAUUACCGACGUCAGC 23 16400 HSV2-UL48-2442 - ACCGCCCCCAU UACCGACGUCAGC 24 16401

HSV2-UL48-2443 - CUGGGAGACGAACUCCGC 18 16402

HSV2-UL48-2444 - CCUGGGAGACGAACUCCGC 19 16403

HSV2-UL48-862 - GCCUGGGAGACGAACUCCGC 20 6248

HSV2-UL48-2445 - AGCCUGGGAGACGAACUCCGC 21 16404

HSV2-UL48-2446 - CAGCCUGGGAGACGAACUCCGC 22 16405

HSV2-UL48-2447 - UCAGCCUGGGAGACGAACUCCGC 23 16406

HSV2-UL48-2448 - GUCAGCCUGGGAGACGAACUCCGC 24 16407

HSV2-UL48-2449 - GAACUCCGCCUGGACGGC 18 16408

HSV2-UL48-2450 - CGAACUCCGCCUGGACGGC 19 16409

HSV2-UL48-864 - ACGAACUCCGCCUGGACGGC 20 6250

HSV2-UL48-2451 - GACGAACUCCGCCUGGACGGC 21 16410

HSV2-UL48-2452 - AG ACGAACUCCGCCUGGACGGC 22 16411

HSV2-UL48-2453 - GAG ACGAACUCCGCCUGGACGGC 23 16412

HSV2-UL48-2454 - GGAG ACGAACUCCGCCUGGACGGC 24 16413

HSV2-UL48-2455 - AGGGCGAGUCCGUCAUGC 18 16414

HSV2-UL48-2456 - GAGGGCGAGUCCGUCAUGC 19 16415

HSV2-UL48-276 - GGAGGGCGAGUCCGUCAUGC 20 5758

HSV2-UL48-2457 - CGGAGGGCGAGUCCGUCAUGC 21 16416

HSV2-UL48-2458 - UCGGAGGGCGAGUCCG UCAUGC 22 16417

HSV2-UL48-2459 - CUCGG AGGGCGAGUCCG UCAUGC 23 16418

HSV2-UL48-2460 - CCUCGGAGGGCGAGUCCGUCAUGC 24 16419

HSV2-UL48-2461 - ACUUCGACU UGGAGAUGC 18 16420

HSV2-UL48-2462 - GACU UCGACU UGGAGAUGC 19 16421

HSV2-UL48-301 - CGACUUCGACU UGGAGAUGC 20 5783

HSV2-UL48-2463 - ACG ACUUCGACUUGGAGAUGC 21 16422

HSV2-UL48-2464 - GACG ACUUCGACUUGGAGAUGC 22 16423

HSV2-UL48-2465 - GG ACG ACUUCGACUUGGAGAUGC 23 16424

HSV2-UL48-2466 - UGGACGACUUCGACUUGGAGAUGC 24 16425

HSV2-UL48-2467 - UGGAGGCCCGGCGACUGC 18 16426

HSV2-UL48-2468 - GUGGAGGCCCGGCGACUGC 19 16427

HSV2-UL48-255 - CG UGGAGGCCCGGCGACUGC 20 5737

HSV2-UL48-2469 - CCG UGGAGGCCCGGCGACUGC 21 16428

HSV2-UL48-2470 - CCCGUGGAGGCCCGGCGACUGC 22 16429

HSV2-UL48-2471 - UCCCGUGGAGGCCCGGCGACUGC 23 16430

HSV2-UL48-2472 - UUCCCGUGGAGGCCCGGCGACUGC 24 16431

HSV2-UL48-2473 - GCCGCAACCGCGACCUGC 18 16432

HSV2-UL48-2474 - GGCCGCAACCGCGACCUGC 19 16433

HSV2-UL48-236 - GGGCCGCAACCGCGACCUGC 20 5718

HSV2-UL48-2475 - GGGGCCGCAACCGCGACCUGC 21 16434

HSV2-UL48-2476 - CGGGGCCGCAACCGCGACCUGC 22 16435

HSV2-UL48-2477 - GCGGGGCCGCAACCGCGACCUGC 23 16436

HSV2-UL48-2478 - UGCGGGGCCGCAACCGCGACCUGC 24 16437

HSV2-UL48-2479 - ACGGAUCGCUCACCGUGC 18 16438

HSV2-UL48-2480 - AACGGAUCGCUCACCG UGC 19 16439

HSV2-UL48-249 - CAACGGAUCGCUCACCGUGC 20 5731 HSV2-UL48-2481 - UCAACGGAUCGCUCACCGUGC 21 16440

HSV2-UL48-2482 - AUCAACGGAUCGCUCACCGUGC 22 16441

HSV2-UL48-2483 - UAUCAACGGAUCGCUCACCGUGC 23 16442

HSV2-UL48-2484 - UUAUCAACGGAUCGCUCACCGUGC 24 16443

HSV2-UL48-2485 - AAUUACGGAUCGACAAUC 18 16444

HSV2-UL48-2486 - CAAUUACGGAUCGACAAUC 19 16445

HSV2-UL48-851 - ACAAUUACGGAUCGACAAUC 20 6241

HSV2-UL48-2487 - AACAAUUACGGAUCGACAAUC 21 16446

HSV2-UL48-2488 - A A AC A A UUACGGAUCG AC A A U C 22 16447

HSV2-UL48-2489 - GAAACAAUUACGGAUCGACAAUC 23 16448

HSV2-UL48-2490 - AGAAACAAUUACGGAUCGACAAUC 24 16449

HSV2-UL48-2491 - AUGCUGCGCACCACGAUC 18 16450

HSV2-UL48-2492 - GAUGCUGCGCACCACGAUC 19 16451

HSV2-UL48-807 - AGAUGCUGCGCACCACGAUC 20 6210

HSV2-UL48-2493 - GAG AUGCUGCGCACCACGAUC 21 16452

HSV2-UL48-2494 - GG AG AUGCUGCGCACCACGAUC 22 16453

HSV2-UL48-2495 - GGG AG AUGCUGCGCACCACGAUC 23 16454

HSV2-UL48-2496 - CGGGAGAUGCUGCGCACCACGAUC 24 16455

HSV2-UL48-2497 - GAGGGCCUGCUCGACCUC 18 16456

HSV2-UL48-2498 - CGAGGGCCUGCUCGACCUC 19 16457

HSV2-UL48-852 - UCGAGGGCCUGCUCGACCUC 20 6242

HSV2-UL48-2499 - AUCGAGGGCCUGCUCGACCUC 21 16458

HSV2-UL48-2500 - AAUCGAGGGCCUGCUCGACCUC 22 16459

HSV2-UL48-2501 - CAAUCGAGGGCCUGCUCGACCUC 23 16460

HSV2-UL48-2502 - ACAAUCGAGGGCCUGCUCGACCUC 24 16461

HSV2-UL48-2503 - G G G AG CU AA ACCAC AU U C 18 16462

HSV2-UL48-2504 - CGGG AGCU AAACCACAU UC 19 16463

HSV2-UL48-826 - GCGGGAGCUAAACCACAUUC 20 6222

HSV2-UL48-2505 - UGCGGG AGCU AAACCACAU UC 21 16464

HSV2-UL48-2506 - CUGCGGG AGCU AAACCACAU UC 22 16465

HSV2-UL48-2507 - ACUGCGGG AGCU AAACCACAU UC 23 16466

HSV2-UL48-2508 - GACUGCGGGAGCUAAACCACAUUC 24 16467

HSV2-UL48-2509 - CUGCUGAUCCGGGCCAAG 18 16468

HSV2-UL48-2510 - GCUGCUGAUCCGGGCCAAG 19 16469

HSV2-UL48-838 - UGCUGCUGAUCCGGGCCAAG 20 6231

HSV2-UL48-2511 - AUGCUGCUGAUCCGGGCCAAG 21 16470

HSV2-UL48-2512 - UAUGCUGCUGAUCCGGGCCAAG 22 16471

HSV2-UL48-2513 - UUAUGCUGCUGAUCCGGGCCAAG 23 16472

HSV2-UL48-2514 - U UUAUGCUGCUGAUCCGGGCCAAG 24 16473

HSV2-UL48-2515 - CCGACGUCAGCCUGGGAG 18 16474

HSV2-UL48-2516 - ACCGACGUCAGCCUGGGAG 19 16475

HSV2-UL48-861 - UACCGACGUCAGCCUGGGAG 20 6247

HSV2-UL48-2517 - UU ACCGACGUCAGCCUGGGAG 21 16476

HSV2-UL48-2518 - AU UACCGACGUCAGCCUGGGAG 22 16477

HSV2-UL48-2519 - CAU UACCGACG UCAGCCUGGGAG 23 16478

HSV2-UL48-2520 - CCAUUACCGACGUCAGCCUGGGAG 24 16479 HSV2-UL48-2521 - ACGAACUCCGCCUGGACG 18 16480

HSV2-UL48-2522 - GACGAACUCCGCCUGGACG 19 16481

HSV2-UL48-863 - AG ACGAACUCCGCCUGGACG 20 6249

HSV2-UL48-2523 - GAG ACGAACUCCGCCUGGACG 21 16482

HSV2-UL48-2524 - GGAG ACGAACUCCGCCUGGACG 22 16483

HSV2-UL48-2525 - GGG AG ACGAACUCCGCCUGGACG 23 16484

HSV2-UL48-2526 - UGGGAGACGAACUCCGCCUGGACG 24 16485

HSV2-UL48-2527 - UCCUAUGGGCCGCGUACG 18 16486

HSV2-UL48-2528 - AUCCUAUGGGCCGCGUACG 19 16487

HSV2-UL48-812 - GAUCCUAUGGGCCGCGUACG 20 6215

HSV2-UL48-2529 - AG AUCCUAUGGGCCGCGUACG 21 16488

HSV2-UL48-2530 - GAG AUCCUAUGGGCCGCGUACG 22 16489

HSV2-UL48-2531 - CGAG AUCCUAUGGGCCGCGUACG 23 16490

HSV2-UL48-2532 - GCGAGAUCCUAUGGGCCGCGUACG 24 16491

HSV2-UL48-2533 - CCGUGCGGGGAGUUCCCG 18 16492

HSV2-UL48-2534 - ACCGUGCGGGGAGUUCCCG 19 16493

HSV2-UL48-251 - CACCGUGCGGGGAGU UCCCG 20 5733

HSV2-UL48-2535 - UCACCGUGCGGGGAGU UCCCG 21 16494

HSV2-UL48-2536 - CUCACCGUGCGGGGAGUUCCCG 22 16495

HSV2-UL48-2537 - GCUCACCGUGCGGGGAGUUCCCG 23 16496

HSV2-UL48-2538 - CGCUCACCGUGCGGGGAGU UCCCG 24 16497

HSV2-UL48-2539 - GCAUGUCGUU UCUCUCCG 18 16498

HSV2-UL48-2540 - CGCAUGUCGUU UCUCUCCG 19 16499

HSV2-UL48-290 - GCGCAUGUCGU UUCUCUCCG 20 5772

HSV2-UL48-2541 - CGCGCAUGUCGUUUCUCUCCG 21 16500

HSV2-UL48-2542 - CCGCGCAUG UCGU U UCUCUCCG 22 16501

HSV2-UL48-2543 - GCCGCGCAUGUCGU UUCUCUCCG 23 16502

HSV2-UL48-2544 - CGCCGCGCAUGUCGUUUCUCUCCG 24 16503

HSV2-UL48-2545 - AACUCCGCCUGGACGGCG 18 16504

HSV2-UL48-2546 - GAACUCCGCCUGGACGGCG 19 16505

HSV2-UL48-296 - CGAACUCCGCCUGGACGGCG 20 5778

HSV2-UL48-2547 - ACG AACUCCGCCUGGACGGCG 21 16506

HSV2-UL48-2548 - GACG AACUCCGCCUGGACGGCG 22 16507

HSV2-UL48-2549 - AGACGAACUCCGCCUGGACGGCG 23 16508

HSV2-UL48-2550 - GAG ACG AACUCCGCCUGGACGGCG 24 16509

HSV2-UL48-2551 - GUGCGAAGUGCGGCGGCG 18 16510

HSV2-UL48-2552 - GGUGCGAAGUGCGGCGGCG 19 16511

HSV2-UL48-831 - UGGUGCGAAGUGCGGCGGCG 20 6226

HSV2-UL48-2553 - CUGGUGCGAAGUGCGGCGGCG 21 16512

HSV2-UL48-2554 - GCUGGUGCGAAGUGCGGCGGCG 22 16513

HSV2-UL48-2555 - CGCUGGUGCGAAGUGCGGCGGCG 23 16514

HSV2-UL48-2556 - CCGCUGGUGCGAAG UGCGGCGGCG 24 16515

HSV2-UL48-2557 - CUGGUGCGAAGUGCGGCG 18 16516

HSV2-UL48-2558 - GCUGGUGCGAAGUGCGGCG 19 16517

HSV2-UL48-829 - CGCUGGUGCGAAGUGCGGCG 20 6225

HSV2-UL48-2559 - CCGCUGGUGCGAAGUGCGGCG 21 16518 HSV2-UL48-2560 - CCCGCUGGUGCGAAGUGCGGCG 22 16519

HSV2-UL48-2561 - UCCCGCUGGUGCGAAGUGCGGCG 23 16520

HSV2-UL48-2562 - CUCCCGCUGGUGCGAAGUGCGGCG 24 16521

HSV2-UL48-2563 - UCCAGCGUCGCGACCUCG 18 16522

HSV2-UL48-2564 - CUCCAGCGUCGCGACCUCG 19 16523

HSV2-UL48-841 - ACUCCAGCGUCGCGACCUCG 20 6233

HSV2-UL48-2565 - UACUCCAGCGUCGCGACCUCG 21 16524

HSV2-UL48-2566 - GUACUCCAGCGUCGCGACCUCG 22 16525

HSV2-UL48-2567 - CGUACUCCAGCGUCGCGACCUCG 23 16526

HSV2-UL48-2568 - UCGUACUCCAGCGUCGCGACCUCG 24 16527

HSV2-UL48-2569 - AUGACGCUCCUGCGGAGG 18 16528

HSV2-UL48-2570 - GAUGACGCUCCUGCGGAGG 19 16529

HSV2-UL48-855 - CGAUGACGCUCCUGCGGAGG 20 6244

HSV2-UL48-2571 - ACG AUGACGCUCCUGCGGAGG 21 16530

HSV2-UL48-2572 - GACG AUGACGCUCCUGCGGAGG 22 16531

HSV2-UL48-2573 - CGACG AUGACGCUCCUGCGGAGG 23 16532

HSV2-UL48-2574 - ACG ACG AUGACGCUCCUGCGGAGG 24 16533

HSV2-UL48-2575 - GCACGCGUAUAGCCGCGG 18 16534

HSV2-UL48-2576 - AGCACGCGUAUAGCCGCGG 19 16535

HSV2-UL48-847 - GAGCACGCGUAUAGCCGCGG 20 6237

HSV2-UL48-2577 - GG AGCACGCGUAUAGCCGCGG 21 16536

HSV2-UL48-2578 - GGG AGCACGCGUAUAGCCGCGG 22 16537

HSV2-UL48-2579 - CGGGAGCACGCGUAUAGCCGCGG 23 16538

HSV2-UL48-2580 - GCGGGAGCACGCGUAUAGCCGCGG 24 16539

HSV2-UL48-2581 - UGCGAAG UGCGGCGGCGG 18 16540

HSV2-UL48-2582 - GUGCGAAGUGCGGCGGCGG 19 16541

HSV2-UL48-260 - GG UGCGAAGUGCGGCGGCGG 20 5742

HSV2-UL48-2583 - UGG UGCGAAGUGCGGCGGCGG 21 16542

HSV2-UL48-2584 - CUGGUGCGAAGUGCGGCGGCGG 22 16543

HSV2-UL48-2585 - GCUGGUGCGAAGUGCGGCGGCGG 23 16544

HSV2-UL48-2586 - CGCUGGUGCGAAGUGCGGCGGCGG 24 16545

HSV2-UL48-2587 - UGGUGCGAAGUGCGGCGG 18 16546

HSV2-UL48-2588 - CUGGUGCGAAGUGCGGCGG 19 16547

HSV2-UL48-259 - GCUGGUGCGAAGUGCGGCGG 20 5741

HSV2-UL48-2589 - CGCUGGUGCGAAGUGCGGCGG 21 16548

HSV2-UL48-2590 - CCGCUGGUGCGAAGUGCGGCGG 22 16549

HSV2-UL48-2591 - CCCGCUGGUGCGAAGUGCGGCGG 23 16550

HSV2-UL48-2592 - UCCCGCUGGUGCGAAGUGCGGCGG 24 16551

HSV2-UL48-2593 - CACCGCCAGGCACACAUG 18 16552

HSV2-UL48-2594 - ACACCGCCAGGCACACAUG 19 16553

HSV2-UL48-232 - UACACCGCCAGGCACACAUG 20 5714

HSV2-UL48-2595 - CUACACCGCCAGGCACACAUG 21 16554

HSV2-UL48-2596 - GCUACACCGCCAGGCACACAUG 22 16555

HSV2-UL48-2597 - AGCU ACACCGCCAGGCACACAUG 23 16556

HSV2-UL48-2598 - CAGCUACACCGCCAGGCACACAUG 24 16557

HSV2-UL48-2599 - GAGGGCGAGUCCGUCAUG 18 16558 HSV2-UL48-2600 - GGAGGGCGAGUCCGUCAUG 19 16559

HSV2-UL48-275 - CGGAGGGCGAGUCCGUCAUG 20 5757

HSV2-UL48-2601 - UCGGAGGGCGAGUCCGUCAUG 21 16560

HSV2-UL48-2602 - CUCGGAGGGCGAGUCCG UCAUG 22 16561

HSV2-UL48-2603 - CCUCGGAGGGCGAGUCCGUCAUG 23 16562

HSV2-UL48-2604 - ACCUCGGAGGGCGAGUCCGUCAUG 24 16563

HSV2-UL48-2605 - GACUUCGACU UGGAGAUG 18 16564

HSV2-UL48-2606 - CGACUUCGACUUGGAGAUG 19 16565

HSV2-UL48-870 - ACGACUUCGACUUGGAGAUG 20 6254

HSV2-UL48-2607 - GACGACUUCGACU UGGAGAUG 21 16566

HSV2-UL48-2608 - GG ACGACUUCGACU UGGAGAUG 22 16567

HSV2-UL48-2609 - UGGACGACU UCGACU UGGAGAUG 23 16568

HSV2-UL48-2610 - CUGGACGACUUCGACUUGGAGAUG 24 16569

HSV2-UL48-2611 - GUGGAGGCCCGGCGACUG 18 16570

HSV2-UL48-2612 - CGUGGAGGCCCGGCGACUG 19 16571

HSV2-UL48-254 - CCGUGGAGGCCCGGCGACUG 20 5736

HSV2-UL48-2613 - CCCGUGGAGGCCCGGCGACUG 21 16572

HSV2-UL48-2614 - UCCCGUGGAGGCCCGGCGACUG 22 16573

HSV2-UL48-2615 - UUCCCGUGGAGGCCCGGCGACUG 23 16574

HSV2-UL48-2616 - GU UCCCGUGGAGGCCCGGCGACUG 24 16575

HSV2-UL48-2617 - GGCCGCAACCGCGACCUG 18 16576

HSV2-UL48-2618 - GGGCCGCAACCGCGACCUG 19 16577

HSV2-UL48-235 - GGGGCCGCAACCGCGACCUG 20 5717

HSV2-UL48-2619 - CGGGGCCGCAACCGCGACCUG 21 16578

HSV2-UL48-2620 - GCGGGGCCGCAACCGCGACCUG 22 16579

HSV2-UL48-2621 - UGCGGGGCCGCAACCGCGACCUG 23 16580

HSV2-UL48-2622 - AUGCGGGGCCGCAACCGCGACCUG 24 16581

HSV2-UL48-2623 - GGCCUCUGCUGCGACCUG 18 16582

HSV2-UL48-2624 - CGGCCUCUGCUGCGACCUG 19 16583

HSV2-UL48-815 - ACGGCCUCUGCUGCGACCUG 20 6217

HSV2-UL48-2625 - GACGGCCUCUGCUGCGACCUG 21 16584

HSV2-UL48-2626 - CGACGGCCUCUGCUGCGACCUG 22 16585

HSV2-UL48-2627 - UCGACGGCCUCUGCUGCGACCUG 23 16586

HSV2-UL48-2628 - UUCGACGGCCUCUGCUGCGACCUG 24 16587

HSV2-UL48-2629 - ACGACGAUGACGCUCCUG 18 16588

HSV2-UL48-2630 - GACGACGAUGACGCUCCUG 19 16589

HSV2-UL48-284 - GG ACGACGAUGACGCUCCUG 20 5766

HSV2-UL48-2631 - CGG ACGACGAUGACGCUCCUG 21 16590

HSV2-UL48-2632 - CCGGACGACGAUGACGCUCCUG 22 16591

HSV2-UL48-2633 - CCCGGACGACGAUGACGCUCCUG 23 16592

HSV2-UL48-2634 - UCCCGGACGACGAUGACGCUCCUG 24 16593

HSV2-UL48-2635 - ACGACCCCGCCCGUCCUG 18 16594

HSV2-UL48-2636 - CACG ACCCCGCCCG UCCUG 19 16595

HSV2-UL48-835 - UCACG ACCCCGCCCG UCCUG 20 6228

HSV2-UL48-2637 - CUCACGACCCCGCCCGUCCUG 21 16596

HSV2-UL48-2638 - CCUCACGACCCCGCCCGUCCUG 22 16597 HSV2-UL48-2639 - CCCUCACGACCCCGCCCG UCCUG 23 16598

HSV2-UL48-2640 - CCCCUCACGACCCCGCCCGUCCUG 24 16599

HSV2-UL48-2641 - UUCGACU UGGAGAUGCUG 18 16600

HSV2-UL48-2642 - CUUCGACUUGGAGAUGCUG 19 16601

HSV2-UL48-303 - ACU UCGACUUGGAGAUGCUG 20 5785

HSV2-UL48-2643 - GACU UCGACUUGGAGAUGCUG 21 16602

HSV2-UL48-2644 - CGACUUCGACUUGGAGAUGCUG 22 16603

HSV2-UL48-2645 - ACG ACUUCGACUUGGAGAUGCUG 23 16604

HSV2-UL48-2646 - GACGACU UCGACUUGGAGAUGCUG 24 16605

HSV2-UL48-2647 - AACGGAUCGCUCACCGUG 18 16606

HSV2-UL48-2648 - CAACGGAUCGCUCACCGUG 19 16607

HSV2-UL48-248 - UCAACGGAUCGCUCACCGUG 20 5730

HSV2-UL48-2649 - AUCAACGGAUCGCUCACCGUG 21 16608

HSV2-UL48-2650 - UAUCAACGGAUCGCUCACCGUG 22 16609

HSV2-UL48-2651 - UUAUCAACGGAUCGCUCACCGUG 23 16610

HSV2-UL48-2652 - UUUAUCAACGGAUCGCUCACCGUG 24 16611

HSV2-UL48-2653 - AC ACCG CC AG G C AC AC A U 18 16612

HSV2-UL48-2654 - UACACCGCCAGGCACACAU 19 16613

HSV2-UL48-802 - CUACACCGCCAGGCACACAU 20 6208

HSV2-UL48-2655 - GCUACACCGCCAGGCACACAU 21 16614

HSV2-UL48-2656 - AGCUACACCGCCAGGCACACAU 22 16615

HSV2-UL48-2657 - CAGCUACACCGCCAGGCACACAU 23 16616

HSV2-UL48-2658 - GCAGCUACACCGCCAGGCACACAU 24 16617

HSV2-UL48-2659 - GGAGGGCGAGUCCGUCAU 18 16618

HSV2-UL48-2660 - CGGAGGGCGAGUCCGUCAU 19 16619

HSV2-UL48-843 - UCGGAGGGCGAGUCCGUCAU 20 6235

HSV2-UL48-2661 - CUCGGAGGGCGAGUCCGUCAU 21 16620

HSV2-UL48-2662 - CCUCGGAGGGCGAGUCCGUCAU 22 16621

HSV2-UL48-2663 - ACCUCGGAGGGCGAGUCCGUCAU 23 16622

HSV2-UL48-2664 - GACCUCGGAGGGCGAGUCCGUCAU 24 16623

HSV2-UL48-2665 - GUACUUUAUGCUGCUGAU 18 16624

HSV2-UL48-2666 - GGUACUUUAUGCUGCUGAU 19 16625

HSV2-UL48-837 - GGGUACUUUAUGCUGCUGAU 20 6230

HSV2-UL48-2667 - UGGGUACUUUAUGCUGCUGAU 21 16626

HSV2-UL48-2668 - CUGGGUACUU UAUGCUGCUGAU 22 16627

HSV2-UL48-2669 - UCUGGGUACU UUAUGCUGCUGAU 23 16628

HSV2-UL48-2670 - CUCUGGGUACUU UAUGCUGCUGAU 24 16629

HSV2-UL48-2671 - CCAUGGGCAUUGACGACU 18 16630

HSV2-UL48-2672 - GCCAUGGGCAU UG ACG ACU 19 16631

HSV2-UL48-886 - UGCCAUGGGCAUUGACGACU 20 6263

HSV2-UL48-2673 - AUGCCAUGGGCAU UGACGACU 21 16632

HSV2-UL48-2674 - GAUGCCAUGGGCAUUGACGACU 22 16633

HSV2-UL48-2675 - CG AUGCCAUGGGCAUUGACGACU 23 16634

HSV2-UL48-2676 - CCGAUGCCAUGGGCAUUGACGACU 24 16635

HSV2-UL48-2677 - CGUGGAGGCCCGGCGACU 18 16636

HSV2-UL48-2678 - CCGUGGAGGCCCGGCGACU 19 16637 HSV2-UL48-823 - CCCGUGGAGGCCCGGCGACU 20 6221

HSV2-UL48-2679 - UCCCGUGGAGGCCCGGCGACU 21 16638

HSV2-UL48-2680 - U UCCCGUGGAGGCCCGGCGACU 22 16639

HSV2-UL48-2681 - GUUCCCGUGGAGGCCCGGCGACU 23 16640

HSV2-UL48-2682 - AGUUCCCGUGGAGGCCCGGCGACU 24 16641

HSV2-UL48-2683 - CCCUGGACGACUUCGACU 18 16642

HSV2-UL48-2684 - GCCCUGGACGACUUCGACU 19 16643

HSV2-UL48-300 - CGCCCUGGACGACUUCGACU 20 5782

HSV2-UL48-2685 - ACGCCCUGGACGACUUCGACU 21 16644

HSV2-UL48-2686 - GACGCCCUGGACGACU UCGACU 22 16645

HSV2-UL48-2687 - CGACGCCCUGGACGACUUCGACU 23 16646

HSV2-UL48-2688 - CCGACGCCCUGGACGACUUCGACU 24 16647

HSV2-UL48-2689 - GGGCCGCAACCGCGACCU 18 16648

HSV2-UL48-2690 - GGGGCCGCAACCGCGACCU 19 16649

HSV2-UL48-804 - CGGGGCCGCAACCGCGACCU 20 6209

HSV2-UL48-2691 - GCGGGGCCGCAACCGCGACCU 21 16650

HSV2-UL48-2692 - UGCGGGGCCGCAACCGCGACCU 22 16651

HSV2-UL48-2693 - AUGCGGGGCCGCAACCGCGACCU 23 16652

HSV2-UL48-2694 - CAUGCGGGGCCGCAACCGCGACCU 24 16653

HSV2-UL48-2695 - ACUCCAGCG UCGCGACCU 18 16654

HSV2-UL48-2696 - UACUCCAGCGUCGCGACCU 19 16655

HSV2-UL48-272 - GUACUCCAGCGUCGCGACCU 20 5754

HSV2-UL48-2697 - CGUACUCCAGCGUCGCGACCU 21 16656

HSV2-UL48-2698 - UCGUACUCCAGCGUCGCGACCU 22 16657

HSV2-UL48-2699 - CUCGUACUCCAGCGUCGCGACCU 23 16658

HSV2-UL48-2700 - ACUCGUACUCCAGCGUCGCGACCU 24 16659

HSV2-UL48-2701 - CAU UACCGACGUCAGCCU 18 16660

HSV2-UL48-2702 - CCAUUACCGACGUCAGCCU 19 16661

HSV2-UL48-293 - CCCAUUACCGACGUCAGCCU 20 5775

HSV2-UL48-2703 - CCCCAUUACCGACGUCAGCCU 21 16662

HSV2-UL48-2704 - CCCCCAU UACCGACGUCAGCCU 22 16663

HSV2-UL48-2705 - GCCCCCAUUACCGACGUCAGCCU 23 16664

HSV2-UL48-2706 - CGCCCCCAUUACCGACGUCAGCCU 24 16665

HSV2-UL48-2707 - UCUGAGCCGCGAGAUCCU 18 16666

HSV2-UL48-2708 - U UCUGAGCCGCGAGAUCCU 19 16667

HSV2-UL48-811 - UUUCUGAGCCGCGAGAUCCU 20 6214

HSV2-UL48-2709 - CUUUCUGAGCCGCGAGAUCCU 21 16668

HSV2-UL48-2710 - UCUUUCUGAGCCGCGAGAUCCU 22 16669

HSV2-UL48-2711 - CUCU UUCUGAGCCGCGAGAUCCU 23 16670

HSV2-UL48-2712 - CCUCUUUCUGAGCCGCGAGAUCCU 24 16671

HSV2-UL48-2713 - GACGACGAUGACGCUCCU 18 16672

HSV2-UL48-2714 - GGACGACGAUGACGCUCCU 19 16673

HSV2-UL48-853 - CGGACGACGAUGACGCUCCU 20 6243

HSV2-UL48-2715 - CCGGACGACGAUGACGCUCCU 21 16674

HSV2-UL48-2716 - CCCGGACGACGAUGACGCUCCU 22 16675

HSV2-UL48-2717 - UCCCGGACGACGAUGACGCUCCU 23 16676 HSV2-UL48-2718 - CUCCCGGACGACGAUGACGCUCCU 24 16677

HSV2-UL48-2719 - CCCGUCUCGUAUGGGGCU 18 16678

HSV2-UL48-2720 - CCCCGUCUCGUAUGGGGCU 19 16679

HSV2-UL48-881 - ACCCCGUCUCGUAUGGGGCU 20 6258

HSV2-UL48-2721 - GACCCCG UCUCGUAUGGGGCU 21 16680

HSV2-UL48-2722 - CGACCCCGUCUCGUAUGGGGCU 22 16681

HSV2-UL48-2723 - ACGACCCCGUCUCGUAUGGGGCU 23 16682

HSV2-UL48-2724 - CACGACCCCGUCUCGUAUGGGGCU 24 16683

HSV2-UL48-2725 - CU UCGACU UGGAGAUGCU 18 16684

HSV2-UL48-2726 - ACU UCGACU UGGAGAUGCU 19 16685

HSV2-UL48-302 - GACUUCGACU UGGAGAUGCU 20 5784

HSV2-UL48-2727 - CGACUUCGACUUGGAGAUGCU 21 16686

HSV2-UL48-2728 - ACGACUUCGACUUGGAGAUGCU 22 16687

HSV2-UL48-2729 - GACG ACUUCGACUUGGAGAUGCU 23 16688

HSV2-UL48-2730 - GGACGACUUCGACUUGGAGAUGCU 24 16689

HSV2-UL48-2731 - ACGUGGACGAUUU UGAGU 18 16690

HSV2-UL48-2732 - GACGUGGACGAUUU UGAGU 19 16691

HSV2-UL48-884 - GGACG UGGACGAUUU UGAGU 20 6261

HSV2-UL48-2733 - UGGACGUGGACGAUU UUGAGU 21 16692

HSV2-UL48-2734 - UUGGACGUGGACGAU U UUGAG U 22 16693

HSV2-UL48-2735 - UUUGGACGUGGACGAU UUUGAGU 23 16694

HSV2-UL48-2736 - CU UUGGACGUGGACGAUUU UGAGU 24

16695

HSV2-UL48-2737 - CAACGGAUCGCUCACCGU 18 16696

HSV2-UL48-2738 - UCAACGGAUCGCUCACCGU 19 16697

HSV2-UL48-817 - AUCAACGGAUCGCUCACCGU 20 6219

HSV2-UL48-2739 - UAUCAACGGAUCGCUCACCGU 21 16698

HSV2-UL48-2740 - UUAUCAACGGAUCGCUCACCGU 22 16699

HSV2-UL48-2741 - UUUAUCAACGGAUCGCUCACCGU 23 16700

HSV2-UL48-2742 - GUUUAUCAACGGAUCGCUCACCGU 24 16701

HSV2-UL48-2743 - CCCACG ACCCCG UCUCG U 18 16702

HSV2-UL48-2744 - ACCCACG ACCCCG UCU CG U 19 16703

HSV2-UL48-879 - G ACCCACG ACCCCG UCUCG U 20 6257

HSV2-UL48-2745 - UGACCCACG ACCCCG UCUCG U 21 16704

HSV2-UL48-2746 - AUGACCCACGACCCCGUCUCGU 22 16705

HSV2-UL48-2747 - AAUG ACCCACG ACCCCG UCUCG U 23 16706

HSV2-UL48-2748 - GAAUG ACCCACG ACCCCG UCUCG U 24 16707

HSV2-UL48-2749 - CCUGAACCUCCCGCUGG U 18 16708

HSV2-UL48-2750 - ACCUGAACCUCCCGCUGGU 19 16709

HSV2-UL48-828 - CACCUGAACCUCCCGCUGGU 20 6224

HSV2-UL48-2751 - GCACCUGAACCUCCCGCUGGU 21 16710

HSV2-UL48-2752 - AGCACCUGAACCUCCCGCUGGU 22 16711

HSV2-UL48-2753 - GAGCACCUGAACCUCCCGCUGGU 23 16712

HSV2-UL48-2754 - CGAGCACCUGAACCUCCCGCUGGU 24 16713

HSV2-UL48-2755 - CAUGGGCAUUGACGACU U 18 16714

HSV2-UL48-2756 - CCAUGGGCAUUGACGACUU 19 16715 HSV2-UL48-315 - GCCAUGGGCAU UGACGACUU 20 5797

HSV2-UL48-2757 - UGCCAUGGGCAUUGACGACUU 21 16716

HSV2-UL48-2758 - AUGCCAUGGGCAU UGACGACU U 22 16717

HSV2-UL48-2759 - GAUGCCAUGGGCAUUGACGACUU 23 16718

HSV2-UL48-2760 - CGAUGCCAUGGGCAUUGACGACUU 24 16719

HSV2-UL48-2761 - AUGGGCAUUGACGACU UU 18 16720

HSV2-UL48-2762 - CAUGGGCAU UG ACG ACU U U 19 16721

HSV2-UL48-316 - CCAUGGGCAUUGACGACUUU 20 5798

HSV2-UL48-2763 - GCCAUGGGCAU UGACGACUUU 21 16722

HSV2-UL48-2764 - UGCCAUGGGCAUUGACGACUU U 22 16723

HSV2-UL48-2765 - AUGCCAUGGGCAUUGACGACU UU 23 16724

HSV2-UL48-2766 - GAUGCCAUGGGCAUUGACGACUUU 24 16725

HSV2-UL48-2767 - CUGCAUCUAUACCUCUU U 18 16726

HSV2-UL48-2768 - UCUGCAUCUAUACCUCU UU 19 16727

HSV2-UL48-809 - U UCUGCAUCUAUACCUCUUU 20 6212

HSV2-UL48-2769 - U UUCUGCAUCUAUACCUCUUU 21 16728

HSV2-UL48-2770 - GU UUCUGCAUCUAUACCUCUU U 22 16729

HSV2-UL48-2771 - UGUU UCUGCAUCUAUACCUCUU U 23 16730

HSV2-UL48-2772 - CUGUUUCUGCAUCUAUACCUCUUU 24 16731

Table 13A provides exemplary targeting domains for knocking out the UL48 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase) Table 13A

Table 13B provides exemplary targeting domains for knocking out the UL48 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 13B

Table 14A provides exemplary targeting domains for knocking out the UL54 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 14A

HSV-UL54-1202 + GUCGGUAGCCAUGUUGU 17 16733

HSV-UL54-93 + GCGAGGCGUCGAGGCUU 17 6516

HSV-UL54-16 - GCUUCCCCCCGGGAACCGCA 20 6439

HSV-UL54-27 + GGAUGCGGUGCCUUGGUCGA 20 6450

HSV-UL54-1203 + GUAGGUCGCCGGGGCUGGGA 20 16734

HSV-UL54-11 - GAUCCGCCACCCGCGACCAC 20 6434

HSV-UL54-28 + GAUGCGGUGCCUUGGUCGAC 20 6451

HSV-UL54-121 - GGCGAUUCCCAAAGGUCCCC 20 6544

HSV-UL54-22 - GGCCGGGGUCGAUACGGCCC 20 6445

HSV-UL54-25 + GGCCGUAUCGACCCCGGCCC 20 6448

HSV-UL54-150 + GGUGUGGAGUCGGCGCCGCC 20 6573

HSV-UL54-1204 + GGGCGUUUCUGGAGACGCGC 20 16735

HSV-UL54-1205 + GUAGCCAUGUUGUAGGUCGC 20 16736

HSV-UL54-40 + GACCACACGCCGGUGGUCGC 20 6463

HSV-UL54-1206 + GUU UCUGGAGACGCGCCGGC 20 16737

HSV-UL54-32 + GGUCGACGGGGGUUGGAUGC 20 6455

HSV-UL54-169 + GGGCGAGGCGUCGAGGCUUC 20 6592

HSV-UL54-114 - GGAGUGUUCCUCGUCGGACG 20 6537

HSV-UL54-151 + GUGUGGAGUCGGCGCCGCCG 20 6574

HSV-UL54-42 + GGCGAGGCGUCGAGGCUUCG 20 6465

HSV-UL54-134 - GGAACCGCACGGGGGGAAGG 20 6557

HSV-UL54-15 - GGUCGCGCCUCGGGACCAGG 20 6438

HSV-UL54-38 + GAGGCGCGACCACACGCCGG 20 6461

HSV-UL54-178 + GCGUCCUCGGGGCGGGCCGG 20 6601

HSV-UL54-43 + GCGAGGCGUCGAGGCUUCGG 20 6466

HSV-UL54-23 + GCGCCGGCGGGGUUUUGGUG 20 6446

HSV-UL54-50 + GUCCUCCUCGAGCUCGCUGU 20 6473

HSV-UL54-45 + GGGCGGGCCGGGGGACCUUU 20 6468

HSV-UL54-1207 + GAGACGCGCCGGCGGGGUUU 20 16738

Table 14B provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 14B

HSV-UL54-218 - CCAACCCCCG U CG ACCA 17 6641

HSV-UL54-79 + UGCGGUGCCUUGGUCGA 17 6502

HSV-UL54-59 - ACCCCUGCGGAGACGGA 17 6482

HSV-UL54-73 - CGGGGCCGGGGUCGAUA 17 6496

HSV-UL54-103 + CGAGCUCGCUGUCGGAC 17 6526

HSV-UL54-76 + CGGGGCCGUAUCGACCC 17 6499

HSV-UL54-74 - CGGGGUCGAUACGGCCC 17 6497

HSV-UL54-77 + CGUAUCGACCCCGGCCC 17 6500

HSV-UL54-242 + CCCCCCGUGCGGUUCCC 17 6665

HSV-UL54-241 + UCCCCCCGUGCGGUUCC 17 6664

HSV-UL54-194 - CCG AG U CCGACAG CAG C 17 6617

HSV-UL54-232 + UGUGGAGUCGGCGCCGC 17 6655

HSV-UL54-1208 + CGUUUCUGGAGACGCGC 17 16739

HSV-UL54-92 + CACACGCCGGUGGUCGC 17 6515

HSV-UL54-1209 + UCUGGAGACGCGCCGGC 17 16740

HSV-UL54-1210 + UUGUAGGUCGCCGGGGC 17 16741

HSV-UL54-84 + CGACGGGGGUUGGAUGC 17 6507

HSV-UL54-252 + CGAGGCGUCGAGGCUUC 17 6675

HSV-UL54-99 + CAUGUCCUCGUCCGACG 17 6522

HSV-UL54-81 + CGGUGCCUUGGUCGACG 17 6504

HSV-UL54-243 + CCCCCGUGCGGUUCCCG 17 6666

HSV-UL54-1211 + CAUGUUGUAGGUCGCCG 17 16742

HSV-UL54-195 - CG AG U CCGACAG CAGCG 17 6618

HSV-UL54-1212 + CUGGAGACGCGCCGGCG 17 16743

HSV-UL54-101 + CGCUGCUGUCGGACUCG 17 6524

HSV-UL54-67 - CGCGCCUCGGGACCAGG 17 6490

HSV-UL54-70 - CCCCGGGAACCGCACGG 17 6493

HSV-UL54-86 + CCCCGUGCGGUUCCCGG 17 6509

HSV-UL54-1213 + UUCUGGAGACGCGCCGG 17 16744

HSV-UL54-95 + AGGCGUCGAGGCUUCGG 17 6518

HSV-UL54-245 + ACGCCGGUGGUCGCGGG 17 6668

HSV-UL54-240 + UCGACGGGGGUUGGAUG 17 6663

HSV-UL54-64 - CCGCGACCACCGGCGUG 17 6487

HSV-UL54-100 + CCCGCUGCUGUCGGACU 17 6523

HSV-UL54-1214 + UGUAGGUCGCCGGGGCU 17 16745

HSV-UL54-247 + CGCGGGUGGCGGAUCGU 17 6670

HSV-UL54-196 - CGGGGAGUGUUCCUCGU 17 6619

HSV-UL54-102 + CUCCUCGAGCUCGCUGU 17 6525

HSV-UL54-97 + CGGGCCGGGGGACCUUU 17 6520

HSV-UL54-1215 + ACGCGCCGGCGGGGUUU 17 16746

HSV-UL54-9 - AUCGACGCGGCGAU UCCCAA 20 6432

HSV-UL54-6 - UUCCUCGUCGGACGAGGACA 20 6429

HSV-UL54-1216 - UCCAGAAACGCCCACAACCA 20 16747

HSV-UL54-135 - CAU CCAACCCCCG U CG ACCA 20 6558

HSV-UL54-181 + UCCGCCCCUCCGUCUCCGCA 20 6604

HSV-UL54-116 - AUGGAAGACCCCUGCGGAGA 20 6539 HSV-UL54-46 + UUUGGGAAUCGCCGCGUCGA 20 6469

HSV-UL54-7 - AAGACCCCUGCGGAGACGGA 20 6430

HSV-UL54-133 - CCGGGAACCGCACGGGGGGA 20 6556

HSV-UL54-21 - CGCCGGGGCCGGGGUCGAUA 20 6444

HSV-UL54-132 - CUUCCCCCCGGGAACCGCAC 20 6555

HSV-UL54-14 - UGUGGUCGCGCCUCGGGACC 20 6437

HSV-UL54-24 + CGCCGGGGCCGUAUCGACCC 20 6447

HSV-UL54-130 - CCAGGCGGUCGGCUUCCCCC 20 6553

HSV-UL54-159 + CUUCCCCCCGUGCGGUUCCC 20 6582

HSV-UL54-1217 + UAGCCAUGUUGUAGGUCGCC 20 16748

HSV-UL54-158 + CCUUCCCCCCGUGCGGUUCC 20 6581

HSV-UL54-111 - ACCCCGAGUCCGACAGCAGC 20 6534

HSV-UL54-37 + CCCGAGGCGCGACCACACGC 20 6460

HSV-UL54-170 + CGUCGAGGCUUCGGGGGUGC 20 6593

HSV-UL54-184 + UCCCCGCUGCUGUCGGACUC 20 6607

HSV-UL54-13 - CCGGCGUGUGGUCGCGCCUC 20 6436

HSV-UL54-44 + UCGGGGGUGCCGGCGUCCUC 20 6467

HSV-UL54-5 - CGAGCUCGAGGAGGACGCUC 20 6428

HSV-UL54-182 + CCG CCCCU CCG UCU CCG CAG 20 6605

HSV-UL54-17 - UUCCCCCCGGGAACCGCACG 20 6440

HSV-UL54-47 + UUCCAUGUCCUCGUCCGACG 20 6470

HSV-UL54-8 - AGGGGCGGAGGCCAUCGACG 20 6431

HSV-UL54-29 + AUGCGGUGCCU UGGUCGACG 20 6452

HSV-UL54-136 - ACCAAGGCACCGCAUCCCCG 20 6559

HSV-UL54-36 + AAGCCGACCGCCUGGUCCCG 20 6459

HSV-UL54-160 + UUCCCCCCGUGCGGUUCCCG 20 6583

HSV-UL54-1218 + AGCCAUGUUGUAGGUCGCCG 20 16749

HSV-UL54-145 - CGAGGUCGCCGCCGGGGCCG 20 6568

HSV-UL54-112 - CCCCGAGUCCGACAGCAGCG 20 6535

HSV-UL54-124 - CGACGCCUCGCCCGGCAGCG 20 6547

HSV-UL54-1219 + UUUCUGGAGACGCGCCGGCG 20 16750

HSV-UL54-139 - CAUCCCCGAGGCGGGCGGCG 20 6562

HSV-UL54-49 + CCCCGCUGCUGUCGGACUCG 20 6472

HSV-UL54-3 - CCUGUCCGACAGCGAGCUCG 20 6426

HSV-UL54-39 + CGACCACACGCCGGUGGUCG 20 6462

HSV-UL54-272 - AGAAACGCCCACAACCAAGG 20 6695

HSV-UL54-137 - AAGGCACCGCAUCCCCGAGG 20 6560

HSV-UL54-18 - UCCCCCCGGGAACCGCACGG 20 6441

HSV-UL54-30 + UGCGGUGCCUUGGUCGACGG 20 6453

HSV-UL54-146 - CGGGGUCGAUACGGCCCCGG 20 6569

HSV-UL54-26 + CGUAUCGACCCCGGCCCCGG 20 6449

HSV-UL54-34 + UCCCCCCGUGCGGUUCCCGG 20 6457

HSV-UL54-1220 + CGUUUCUGGAGACGCGCCGG 20 16751

HSV-UL54-35 + CCCCCCGUGCGGUUCCCGGG 20 6458

HSV-UL54-162 + CACACGCCGGUGGUCGCGGG 20 6585

HSV-UL54-20 - CACCGCAUCCCCGAGGCGGG 20 6443 HSV-UL54-157 + UGGUCGACGGGGGUUGGAUG 20 6580

HSV-UL54-33 + CGGGCCACCUUCCCCCCGUG 20 6456

HSV-UL54-12 - CACCCGCGACCACCGGCGUG 20 6435

HSV-UL54-2 - UAUGCUAAUCGACCUAGGAU 20 6425

HSV-UL54-1 - AUUGAUAUGCUAAUCGACCU 20 6424

HSV-UL54-128 - ACCGGCGUGUGGUCGCGCCU 20 6551

HSV-UL54-52 + UGUCGGACAGGUCCAAUCCU 20 6475

HSV-UL54-171 + UUCGGGGGUGCCGGCGUCCU 20 6594

HSV-UL54-1221 + UGUUGUAGGUCGCCGGGGCU 20 16752

HSV-UL54-113 - CAGCGGGGAGUGUUCCUCGU 20 6536

HSV-UL54-1222 + AAUGUCGGUAGCCAUGUUGU 20 16753

HSV-UL54-41 + CGGGCGAGGCGUCGAGGCUU 20 6464

Table 14C provides exemplary targeting domains for knocking out the UL54 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 14C

HSV-UL54-248 + GCUCCCCGCCGCGCUGC 17 6671

HSV-UL54-96 + GGGGUGCCGGCGUCCUC 17 6519

HSV-UL54-57 - GCUCGAGGAGGACGCUC 17 6480

HSV-UL54-260 + GUCCUCGGGGCGGGCCG 17 6683

HSV-UL54-228 - GGUCGCCGCCGGGGCCG 17 6651

HSV-UL54-255 + GGGUGCCGGCGUCCUCG 17 6678

HSV-UL54-55 - GUCCGACAGCGAGCUCG 17 6478

HSV-UL54-220 - GCACCGCAUCCCCGAGG 17 6643

HSV-UL54-200 - GACCCCUGCGGAGACGG 17 6623

HSV-UL54-198 - GACAUGGAAGACCCCUG 17 6621

HSV-UL54-85 + G CCACCU U CCCCCCG U G 17 6508

HSV-UL54-235 + GACCUCGCCGCCCGCCU 17 6658

HSV-UL54-211 - GGCGUGUGGUCGCGCCU 17 6634

HSV-UL54-254 + GGGGGUGCCGGCGUCCU 17 6677

HSV-UL54-231 + GGGUUUUGGUGUGGAGU 17 6654

HSV-UL54-270 + GAUUAGCAUAUCAAUGU 17 6693

HSV-UL54-1227 + GCGACCCCCUUGGUUGU 17 16758

HSV-UL54-262 + GCGGGCCGGGGGACCUU 17 6685

HSV-UL54-109 - GCUCUGGAGCGGGACGAGGA 20 6532

HSV-UL54-144 - GCGAGGUCGCCGCCGGGGCC 20 6567

HSV-UL54-140 - GCGGGCGGCGAGGUCGCCGC 20 6563

HSV-UL54-126 - GCCUCGCCCGGCAGCGCGGC 20 6549

HSV-UL54-143 - GGCGAGGUCGCCGCCGGGGC 20 6566

HSV-UL54-174 + GGUGCCGGCGUCCUCGGGGC 20 6597

HSV-UL54-1228 + GUCGCCGGGGCUGGGAUGGC 20 16759

HSV-UL54-153 + GCGACCUCGCCGCCCGCCUC 20 6576

HSV-UL54-110 - GACCCCGAGUCCGACAGCAG 20 6533

HSV-UL54-107 - GGACGCUCUGGAGCGGGACG 20 6530

HSV-UL54-142 - GGGCGGCGAGGUCGCCGCCG 20 6565

HSV-UL54-177 + GGCGUCCUCGGGGCGGGCCG 20 6600

HSV-UL54-4 - GUCCGACAGCGAGCUCGAGG 20 6427

HSV-UL54-117 - GAAGACCCCUGCGGAGACGG 20 6540

HSV-UL54-173 + GGGUGCCGGCGUCCUCGGGG 20 6596

HSV-UL54-1229 + GGUCGCCGGGGCUGGGAUGG 20 16760

HSV-UL54-115 - GAGGACAUGGAAGACCCCUG 20 6538

HSV-UL54-152 + GGCGACCUCGCCGCCCGCCU 20 6575

HSV-UL54-156 + GCCUCGGGGAUGCGGUGCCU 20 6579

HSV-UL54-148 + GCGGGGUUUUGGUGUGGAGU 20 6571

HSV-UL54-164 + GGUCGCGGGUGGCGGAUCGU 20 6587

HSV-UL54-129 - GCGCCUCGGGACCAGGCGGU 20 6552

HSV-UL54-31 + GUGCCUUGGUCGACGGGGGU 20 6454

HSV-UL54-187 + GUCGAUUAGCAUAUCAAUGU 20 6610

HSV-UL54-183 + GGAACACUCCCCGCUGCUGU 20 6606

HSV-UL54-179 + GGGGCGGGCCGGGGGACCUU 20 6602 Table 14D provides exemplary targeting domains for knocking out the UL54 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 14D

HSV-UL54-207 - CGCCUCGCCCGGCAGCG 17 6630

HSV-UL54-210 - CGCCCGGCAGCGCGGCG 17 6633

HSV-UL54-222 - CCCCGAGGCGGGCGGCG 17 6645

HSV-UL54-250 + CGCCGCGCUGCCGGGCG 17 6673

HSV-UL54-237 + CCUCGCCGCCCGCCUCG 17 6660

HSV-UL54-251 + UGCCGGGCGAGGCGUCG 17 6674

HSV-UL54-268 + CGGACUCGGGGUCGUCG 17 6691

HSV-UL54-91 + CCACACGCCGGUGGUCG 17 6514

HSV-UL54-468 - AACG CCCACAACCAAG G 17 6889

HSV-UL54-217 - ACCGCACGGGGGGAAGG 17 6640

HSV-UL54-191 - UCUGGAGCGGGACGAGG 17 6614

HSV-UL54-56 - CGACAGCGAGCUCGAGG 17 6479

HSV-UL54-78 + AUCGACCCCGGCCCCGG 17 6501

HSV-UL54-261 + UCCUCGGGGCGGGCCGG 17 6684

HSV-UL54-208 - CUCGCCCGGCAGCGCGG 17 6631

HSV-UL54-269 + ACUCGGGGUCGUCGCGG 17 6692

HSV-UL54-203 - CGGAGACGGAGGGGCGG 17 6626

HSV-UL54-71 - CCCGGGAACCGCACGGG 17 6494

HSV-UL54-87 + CCCGUGCGGUUCCCGGG 17 6510

HSV-UL54-72 - CGCAUCCCCGAGGCGGG 17 6495

HSV-UL54-202 - CUGCGGAGACGGAGGGG 17 6625

HSV-UL54-256 + UGCCGGCGUCCUCGGGG 17 6679

HSV-UL54-1233 + CGCCGGGGCUGGGAUGG 17 16764

HSV-UL54-246 + CCGGUGGUCGCGGGUGG 17 6669

HSV-UL54-238 + CGCCCGCCUCGGGGAUG 17 6661

HSV-UL54-75 + CCGGCGGGGUUU UGGUG 17 6498

HSV-UL54-239 + UCGGGGAUGCGGUGCCU 17 6662

HSV-UL54-104 + CGGACAGGUCCAAUCCU 17 6527

HSV-UL54-212 - CCUCGGGACCAGGCGGU 17 6635

HSV-UL54-83 + CCUUGGUCGACGGGGGU 17 6506

HSV-UL54-266 + ACACUCCCCGCUGCUGU 17 6689

HSV-UL54-1234 - CCAGAAACGCCCACAACCAA 20 16765

HSV-UL54-1235 - AG CCCCGG CG ACCU ACAACA 20 16766

HSV-UL54-51 + CCUCGAGCUCGCUGUCGGAC 20 6474

HSV-UL54-131 - CAGGCGGUCGGCUUCCCCCC 20 6554

HSV-UL54-161 + CCGGGGGGAAGCCGACCGCC 20 6584

HSV-UL54-141 - CGGGCGGCGAGGUCGCCGCC 20 6564

HSV-UL54-10 - CGAAGCCUCGACGCCUCGCC 20 6433

HSV-UL54-176 + CGGCGUCCUCGGGGCGGGCC 20 6599

HSV-UL54-166 + CGGCUCCCCGCCGCGCUGCC 20 6589

HSV-UL54-106 - CGAGGAGGACGCUCUGGAGC 20 6529

HSV-UL54-123 - CCGGCCCGCCCCGAGGACGC 20 6546

HSV-UL54-147 - ACUCCACACCAAAACCCCGC 20 6570

HSV-UL54-149 + UGGUGUGGAGUCGGCGCCGC 20 6572

HSV-UL54-180 + CUCCGCCCCUCCGUCUCCGC 20 6603

HSV-UL54-138 - AGGCACCGCAUCCCCGAGGC 20 6561 HSV-UL54-175 + CCGGCGUCCUCGGGGCGGGC 20 6598

HSV-UL54-1236 + AUGUUGUAGGUCGCCGGGGC 20 16767

HSV-UL54-165 + UCGGCUCCCCGCCGCGCUGC 20 6588

HSV-UL54-1237 + CCCUUGGUUGUGGGCGUUUC 20 16768

HSV-UL54-271 - CAGAAACG CCCACAACCAAG 20 6694

HSV-UL54-118 - AGACCCCUGCGGAGACGGAG 20 6541

HSV-UL54-105 - UCGAGGAGGACGCUCUGGAG 20 6528

HSV-UL54-122 - AGGUCCCCCGGCCCGCCCCG 20 6545

HSV-UL54-127 - CCUCGCCCGGCAGCGCGGCG 20 6550

HSV-UL54-167 + CCCCGCCGCGCUGCCGGGCG 20 6590

HSV-UL54-154 + CGACCUCGCCGCCCGCCUCG 20 6577

HSV-UL54-172 + CGGGGGUGCCGGCGUCCUCG 20 6595

HSV-UL54-168 + CGCUGCCGGGCGAGGCGUCG 20 6591

HSV-UL54-185 + UGUCGGACUCGGGGUCGUCG 20 6608

HSV-UL54-108 - CGCUCUGGAGCGGGACGAGG 20 6531

HSV-UL54-125 - CGCCUCGCCCGGCAGCGCGG 20 6548

HSV-UL54-186 + CGGACUCGGGGUCGUCGCGG 20 6609

HSV-UL54-120 - CUGCGGAGACGGAGGGGCGG 20 6543

HSV-UL54-19 - CCCCCCGGGAACCGCACGGG 20 6442

HSV-UL54-119 - CCCCUGCGGAGACGGAGGGG 20 6542

HSV-UL54-163 + ACGCCGGUGGUCGCGGGUGG 20 6586

HSV-UL54-155 + CGCCGCCCGCCUCGGGGAUG 20 6578

HSV-UL54-48 + CUCCCCGCUGCUGUCGGACU 20 6471

Table 14E provides exemplary targeting domains for knocking out the UL54 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a S. pyogenes Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 14E

HSV-UL54-1242 + AAACGGCAUCCCGCCAA 17 16773

HSV-UL54-1243 + CAGGUAGCACUGCAGAA 17 16774

HSV-UL54-1244 + U CGCAG AACCG CCCGAA 17 16775

HSV-UL54-493 - CCCGGUCCCGGAGCGAA 17 6914

HSV-UL54-530 - GGACCCUAUCAUCGGAA 17 6951

HSV-UL54-1245 + UUGCCGUGCACAUAUAA 17 16776

HSV-UL54-1231 - AGAAACGCCCACAACCA 17 16762

HSV-UL54-1246 + CGGGCCGUGAGCGACCA 17 16777

HSV-UL54-1247 + GGGGUCU UGCAUGACCA 17 16778

HSV-UL54-474 - GGCCCCGGCGCCACCCA 17 6895

HSV-UL54-1248 + GCCGGCCCCAACCCCCA 17 16779

HSV-UL54-1249 + GCCAGCACGGGAGCCCA 17 16780

HSV-UL54-521 - GGCCGCGUCGACAGCCA 17 6942

HSV-UL54-1250 + CUCAUCCGCGGACGCCA 17 16781

HSV-UL54-618 + CGCUUGGGUGGCCAGCA 17 7039

HSV-UL54-1251 + GGCGAGGUUUUCCAGCA 17 16782

HSV-UL54-1252 + CAG CACG CAG U CG CG CA 17 16783

HSV-UL54-557 - CAUCCCGGGGGCCUGCA 17 6978

HSV-UL54-563 - GCCCCCUUAUAUGUGCA 17 6984

HSV-UL54-518 - G AAACCCU G G U CGCU CA 17 6939

HSV-UL54-1253 + CGCGUGCGGGGGGGUCA 17 16784

HSV-UL54-1254 + UCGAUGGUGUCGGCAGA 17 16785

HSV-UL54-1255 + UUUCCCAGGAAACCCGA 17 16786

HSV-UL54-1256 + CCGAACGGCGGGGUCGA 17 16787

HSV-UL54-472 - GCGUCGGCGCGGACGGA 17 6893

HSV-UL54-1257 + ACAGCGCCAUCAGCGGA 17 16788

HSV-UL54-490 - ACCCCCCCGCACGCGGA 17 6911

HSV-UL54-479 - GCGAGGCGCGGCGCGGA 17 6900

HSV-UL54-471 - CCCCGCGUCGGCGCGGA 17 6892

HSV-UL54-533 - UCUGCAGUGCUACCUGA 17 6954

HSV-UL54-562 - UCGCGUGUGCGAGCUGA 17 6983

HSV-UL54-488 - GGCCCCCCCUCCGCUGA 17 6909

HSV-UL54-1258 + UAUUCUUGCCUAAAAUA 17 16789

HSV-UL54-1259 + CGCCGCGCGCUCGGAUA 17 16790

HSV-UL54-1260 + CCGCCGUUCCGAUGAUA 17 16791

HSV-UL54-1261 + UUUGCCGUGCACAUAUA 17 16792

HSV-UL54-540 - ACGCCUGUCGGACAUUA 17 6961

HSV-UL54-619 + GCUUGGGUGGCCAGCAC 17 7040

HSV-UL54-1262 + GGCGUCGCCGCGAGCAC 17 16793

HSV-UL54-1263 + GGUCGUCCAGCCCGCAC 17 16794

HSV-UL54-1264 + UUCCCAGGAAACCCGAC 17 16795

HSV-UL54-1265 + UAUCCUUAAUGUCCGAC 17 16796

HSV-UL54-1266 + CAGCUCGCACACGCGAC 17 16797

HSV-UL54-1267 + GCCUUUCGCUCCGGGAC 17 16798

HSV-UL54-1268 + GGGGGCGAUAGUGUGAC 17 16799

HSV-UL54-560 - GGGUCUCAUUGAAAUAC 17 6981 HSV-UL54-1269 + UCGGGCCGUGAGCGACC 17 16800

HSV-UL54-1270 + CCUUUCGCUCCGGGACC 17 16801

HSV-UL54-1271 + AGGGGUCUUGCAUGACC 17 16802

HSV-UL54-517 - G G U U U CCU GGGAAACCC 17 6938

HSV-UL54-528 - UCUGCCGCUCCGCCCCC 17 6949

HSV-UL54-1272 + CCGCCAUGCAGGCCCCC 17 16803

HSV-UL54-504 - CCGCCGCGAACAGCCCC 17 6925

HSV-UL54-1273 + CCCGCCAUGCAGGCCCC 17 16804

HSV-UL54-1274 + UCGUGGCCGCGGGCCCC 17 16805

HSV-UL54-1275 + GGCCAGCACGGGAGCCC 17 16806

HSV-UL54-491 - CGCGGACGGCCGCGCCC 17 6912

HSV-UL54-524 - AAAU CU CAU CG AGG CCC 17 6945

HSV-UL54-473 - GCGCGGACGGACGGCCC 17 6894

HSV-UL54-500 - CUUUGGACGCAGUGCCC 17 6921

HSV-UL54-554 - AUGCACU UUUACAUCCC 17 6975

HSV-UL54-1276 + AGCGACCAGGGUUUCCC 17 16807

HSV-UL54-1277 + U CU CAU CCG CGGACG CC 17 16808

HSV-UL54-561 - ACUGGACACGCACCGCC 17 6982

HSV-UL54-487 - GCCACGAGGCACGCGCC 17 6908

HSV-UL54-1278 + UCGCCGUGGGUGGCGCC 17 16809

HSV-UL54-522 - CGACUGCGUGCUGCGCC 17 6943

HSV-UL54-1279 + GGUUGGCGAGGCGGGCC 17 16810

HSV-UL54-639 + UCAGCGGAGGGGGGGCC 17 7060

HSV-UL54-553 - GAUGCACUUUUACAUCC 17 6974

HSV-UL54-542 - CUUUGUGUUGGUCAUCC 17 6963

HSV-UL54-1280 + CGCGACUGGAACACUCC 17 16811

HSV-UL54-1281 + AGGGCGCCUUUCGCUCC 17 16812

HSV-UL54-482 - AGCAGCUCGACGUCUCC 17 6903

HSV-UL54-492 - CGGCCGCGCCCCGGUCC 17 6913

HSV-UL54-597 + UUCCGAUGAUAGGGUCC 17 7018

HSV-UL54-515 - AGACCCGUCGGGU UUCC 17 6936

HSV-UL54-1282 + CCUCGAUGAGAUUUUCC 17 16813

HSV-UL54-508 - GUGCUGGCCACCCAAGC 17 6929

HSV-UL54-526 - GUCCGCGGAUGAGACGC 17 6947

HSV-UL54-655 + UCCGUCCGCGCCGACGC 17 7076

HSV-UL54-520 - AUUCGCAGCCAACCCGC 17 6941

HSV-UL54-230 - CCACACCAAAACCCCGC 17 6653

HSV-UL54-1283 + CUGUCGACGCGGCCCGC 17 16814

HSV-UL54-1284 + GCGUGCCUCGUGGCCGC 17 16815

HSV-UL54-1208 + CGUUUCUGGAGACGCGC 17 16739

HSV-UL54-1285 + ACUGCAGAAAGGGGCGC 17 16816

HSV-UL54-1286 + CUCGCCGUGGGUGGCGC 17 16817

HSV-UL54-1287 + AAAGUGCAUCGUCUCGC 17 16818

HSV-UL54-1288 + GACGCGGUUGGCGAGGC 17 16819

HSV-UL54-1289 + AG AACCG CCCG AACGG C 17 16820

HSV-UL54-1209 + UCUGGAGACGCGCCGGC 17 16740 HSV-UL54-1290 + CAGGGGCUGUUCGCGGC 17 16821

HSV-UL54-537 - CCGAGGCCUGUGCGGGC 17 6958

HSV-UL54-552 - ACCGUGGGGGU UGGGGC 17 6973

HSV-UL54-559 - CCGGGGGCCUGCAUGGC 17 6980

HSV-UL54-503 - UGCAAGACCCCUUUGGC 17 6924

HSV-UL54-1291 + AAUGAGACCCGCCAUGC 17 16822

HSV-UL54-506 - CCCCUGGGCUCCCGUGC 17 6927

HSV-UL54-532 - AACGGCGGCCGCCGUGC 17 6953

HSV-UL54-1292 + CGCGGCCGUCCGCGUGC 17 16823

HSV-UL54-536 - AGGCCCGAGGCCUGUGC 17 6957

HSV-UL54-1293 + CAGCCCGCACAGGCCUC 17 16824

HSV-UL54-1294 + GAGGGCGCCUU UCGCUC 17 16825

HSV-UL54-1295 + AUGUGCGGUAGAGGCUC 17 16826

HSV-UL54-481 - GAGCAGCUCGACGUCUC 17 6902

HSV-UL54-514 - UGACGCCGAGACCCGUC 17 6935

HSV-UL54-1296 + CCGCGUGCGGGGGGGUC 17 16827

HSV-UL54-1147 + ACAGGCCUCGGGCCUUC 17 7419

HSV-UL54-495 - CAU CG ACCCCG CCG U U C 17 6916

HSV-UL54-661 + UUGGUUGUGGGCGUUUC 17 7082

HSV-UL54-1297 + ACGGCAUCCCGCCAAAG 17 16828

HSV-UL54-1298 + GGUAGCACUGCAGAAAG 17 16829

HSV-UL54-467 - AAACGCCCACAACCAAG 17 6888

HSV-UL54-507 - CGUGCUGGCCACCCAAG 17 6928

HSV-UL54-1299 + UGCCGUGCACAUAUAAG 17 16830

HSV-UL54-1300 + CCAGCACGGGAGCCCAG 17 16831

HSV-UL54-1301 + GUCGAUGGUGUCGGCAG 17 16832

HSV-UL54-1302 + AGGGACAGCGCCAUCAG 17 16833

HSV-UL54-1303 + CAGCGCCAUCAGCGGAG 17 16834

HSV-UL54-480 - CGAGGCGCGGCGCGGAG 17 6901

HSV-UL54-1304 + GGGUCCUGGGGGCGGAG 17 16835

HSV-UL54-1305 + CUGCGAAUGUGCGGUAG 17 16836

HSV-UL54-486 - GGGGGCCCGCGGCCACG 17 6907

HSV-UL54-489 - CCUGACCCCCCCGCACG 17 6910

HSV-UL54-1306 + GUCCGUCCGCGCCGACG 17 16837

HSV-UL54-583 + ACACGCCGCGCUCGACG 17 7004

HSV-UL54-1307 + GGCCUUGGCUGUCGACG 17 16838

HSV-UL54-545 - GAUCGACUACACGACCG 17 6966

HSV-UL54-1308 + CUUUCGCUCCGGGACCG 17 16839

HSV-UL54-519 - CAU U CG CAG CCAACCCG 17 6940

HSV-UL54-534 - UGCUACCUGAAGGCCCG 17 6955

HSV-UL54-1309 + GCUGUCGACGCGGCCCG 17 16840

HSV-UL54-485 - ACGUCUCCGGGGGCCCG 17 6906

HSV-UL54-555 - UGCACUUUUACAUCCCG 17 6976

HSV-UL54-652 + CGCCGUGGGUGGCGCCG 17 7073

HSV-UL54-1192 + CGCGCCGCGCCUCGCCG 17 7448

HSV-UL54-1310 + CGCGUGCCUCGUGGCCG 17 16841 HSV-UL54-600 + CGCCAGCGUCUCAUCCG 17 7021

HSV-UL54-483 - GCAGCUCGACGUCUCCG 17 6904

HSV-UL54-525 - CGAGGCCCUGGCGUCCG 17 6946

HSV-UL54-509 - UGCUGGCCACCCAAGCG 17 6930

HSV-UL54-543 - GCCAACCGCGUCGAGCG 17 6964

HSV-UL54-1311 + CCGUCCGCGCCGACGCG 17 16842

HSV-UL54-497 - AUCCAUAUCCGAGCGCG 17 6918

HSV-UL54-1312 + AAAGGGGCGCAGGCGCG 17 16843

HSV-UL54-476 - CCACCCACGGCGAGGCG 17 6897

HSV-UL54-1313 + GAACCG CCCG AACGG CG 17 16844

HSV-UL54-475 - CGGCGCCACCCACGGCG 17 6896

HSV-UL54-1212 + CUGGAGACGCGCCGGCG 17 16743

HSV-UL54-477 - CACGGCGAGGCGCGGCG 17 6898

HSV-UL54-470 - GCCACCCCGCGUCGGCG 17 6891

HSV-UL54-1314 + GCUCCGGGACCGGGGCG 17 16845

HSV-UL54-527 - CGGAUGAGACGCUGGCG 17 6948

HSV-UL54-1315 + GGCGCAGGCGCGUGGCG 17 16846

HSV-UL54-1316 + GCUCGACGCGGU UGGCG 17 16847

HSV-UL54-1317 + GCGGCCGUCCGCGUGCG 17 16848

HSV-UL54-523 - CCAGGAAAAUCUCAUCG 17 6944

HSV-UL54-1318 + GCCUGGCGCGUGCCUCG 17 16849

HSV-UL54-538 - UGGACGACCUGUGCUCG 17 6959

HSV-UL54-1319 + AGCCCAGGGGCUGUUCG 17 16850

HSV-UL54-1320 + G AU G ACCAACACAAAG G 17 16851

HSV-UL54-468 - AACGCCCACAACCAAGG 17 6889

HSV-UL54-1321 + GCCGUGCACAUAUAAGG 17 16852

HSV-UL54-1322 + CGACGCGGUUGGCGAGG 17 16853

HSV-UL54-1323 + AGCGCCAUCAGCGGAGG 17 16854

HSV-UL54-1324 + CAGAACCGCCCGAACGG 17 16855

HSV-UL54-531 - CCCUAUCAUCGGAACGG 17 6952

HSV-UL54-1325 + GAGGU UUUCCAGCACGG 17 16856

HSV-UL54-556 - GCACUUUUACAUCCCGG 17 6977

HSV-UL54-1213 + UUCUGGAGACGCGCCGG 17 16744

HSV-UL54-484 - CAGCUCGACGUCUCCGG 17 6905

HSV-UL54-510 - GCUGGCCACCCAAGCGG 17 6931

HSV-UL54-1326 + GACAGCGCCAUCAGCGG 17 16857

HSV-UL54-498 - CAUAUCCGAGCGCGCGG 17 6919

HSV-UL54-478 - GGCGAGGCGCGGCGCGG 17 6899

HSV-UL54-1327 + CCAGGGGCUGU UCGCGG 17 16858

HSV-UL54-1328 + CGGCCGUCCGCGUGCGG 17 16859

HSV-UL54-1329 + GCGCCAUCAGCGGAGGG 17 16860

HSV-UL54-511 - CUGGCCACCCAAGCGGG 17 6932

HSV-UL54-1330 + GGCCGUCCGCGUGCGGG 17 16861

HSV-UL54-496 - CGACCCCGCCGUUCGGG 17 6917

HSV-UL54-1331 + CGCCAUCAGCGGAGGGG 17 16862

HSV-UL54-512 - UGGCCACCCAAGCGGGG 17 6933 HSV-UL54-656 + UCCGCGCCGACGCGGGG 17 7077

HSV-UL54-1332 + GCCGUCCGCGUGCGGGG 17 16863

HSV-UL54-1153 + UGAUAGGGUCCUGGGGG 17 7423

HSV-UL54-1333 + CCGCGCCUCGCCGUGGG 17 16864

HSV-UL54-1334 + AAACCCCCCCGCUUGGG 17 16865

HSV-UL54-558 - CCCGGGGGCCUGCAUGG 17 6979

HSV-UL54-1335 + GACUGGAACACUCCUGG 17 16866

HSV-UL54-1336 + CGAUGAUAGGGUCCUGG 17 16867

HSV-UL54-548 - CGACUACACGACCGUGG 17 6969

HSV-UL54-502 - AUGCAAGACCCCU UUGG 17 6923

HSV-UL54-1337 + CCGAUGAUAGGGUCCUG 17 16868

HSV-UL54-547 - UCGACUACACGACCGUG 17 6968

HSV-UL54-1338 + GCGCGGCCGUCCGCGUG 17 16869

HSV-UL54-75 + CCGGCGGGGUUUUGGUG 17 6498

HSV-UL54-1339 + GGUUGGCUGCGAAUGUG 17 16870

HSV-UL54-535 - AAGGCCCGAGGCCUGUG 17 6956

HSV-UL54-1340 + GGCGGAGCGGCAGAUUG 17 16871

HSV-UL54-551 - CACGACCGUGGGGGUUG 17 6972

HSV-UL54-1201 + GGCGACCCCCUUGGUUG 17 16732

HSV-UL54-1341 + UUAUUCUUGCCUAAAAU 17 16872

HSV-UL54-529 - CCCCAGGACCCUAUCAU 17 6950

HSV-UL54-1342 + GCCGCCGUUCCGAUGAU 17 16873

HSV-UL54-1343 + CGGGGUGGCGACCCCCU 17 16874

HSV-UL54-505 - CGCCGCGAACAGCCCCU 17 6926

HSV-UL54-1344 + CCAGCCCGCACAGGCCU 17 16875

HSV-UL54-1345 + GCAGUCGCGCAUGGCCU 17 16876

HSV-UL54-1346 + UCCGAUGAUAGGGUCCU 17 16877

HSV-UL54-516 - GACCCGUCGGGUUUCCU 17 6937

HSV-UL54-1166 + CG U CAAACCCCCCCG CU 17 7433

HSV-UL54-1347 + CUCGACCGCCGCGCGCU 17 16878

HSV-UL54-1348 + AAUGUGCGGUAGAGGCU 17 16879

HSV-UL54-612 + GGAAACCCGACGGGUCU 17 7033

HSV-UL54-546 - AUCGACUACACGACCGU 17 6967

HSV-UL54-513 - UUGACGCCGAGACCCGU 17 6934

HSV-UL54-1349 + GCGCCGCGCCUCGCCGU 17 16880

HSV-UL54-469 - GGGUCGCCACCCCGCGU 17 6890

HSV-UL54-1350 + GCCGCGCUCGACGCGGU 17 16881

HSV-UL54-1351 + CGACGCGGCCCGCGGGU 17 16882

HSV-UL54-549 - UACACGACCGUGGGGGU 17 6970

HSV-UL54-539 - CUCGCGGCGACGCCUGU 17 6960

HSV-UL54-544 - CGUCGAGCGCGGCGUGU 17 6965

HSV-UL54-1352 + GGCGGGGUCGAUGGUGU 17 16883

HSV-UL54-541 - UAUUGCCUCCUUUGUGU 17 6962

HSV-UL54-1227 + GCGACCCCCUUGGUUGU 17 16758

HSV-UL54-501 - GUCAUGCAAGACCCCUU 17 6922

HSV-UL54-499 - CGCAUCAGCGAAAGCUU 17 6920 HSV-UL54-1353 + GUCAAACCCCCCCGCUU 17 16884

HSV-UL54-494 - CCAU CG ACCCCG CCG U U 17 6915

HSV-UL54-550 - ACACGACCGUGGGGGUU 17 6971

HSV-UL54-1215 + ACGCGCCGGCGGGGUU U 17 16746

HSV-UL54-1354 - CUGCAACUCCCUAUUUU 17 16885

HSV-UL54-1355 + GGAAACGGCAUCCCGCCAAA 20 16886

HSV-UL54-1356 + UUCAGGUAGCACUGCAGAAA 20 16887

HSV-UL54-1025 + GGGCUGUUCGCGGCGGGAAA 20 7339

HSV-UL54-1357 + GGCCAGGAUGACCAACACAA 20 16888

HSV-UL54-1234 - CCAG AAACG CCCACAACCAA 20 16765

HSV-UL54-1358 + G G G AAACG G CAU CCCG CCAA 20 16889

HSV-UL54-1359 + CUUCAGGUAGCACUGCAGAA 20 16890

HSV-UL54-1360 + GGAUCGCAGAACCGCCCGAA 20 16891

HSV-UL54-297 - CGCCCCGGUCCCGGAGCGAA 20 6720

HSV-UL54-334 - CCAGGACCCUAUCAUCGGAA 20 6757

HSV-UL54-1361 + UAUUUGCCGUGCACAUAUAA 20 16892

HSV-UL54-1216 - UCCAGAAACGCCCACAACCA 20 16747

HSV-UL54-1362 + GCUCGGGCCGUGAGCGACCA 20 16893

HSV-UL54-1363 + AAAGGGGUCUUGCAUGACCA 20 16894

HSV-UL54-278 - GACGGCCCCGGCGCCACCCA 20 6701

HSV-UL54-1364 + CUCGCCGGCCCCAACCCCCA 20 16895

HSV-UL54-1365 + GUGGCCAGCACGGGAGCCCA 20 16896

HSV-UL54-325 - GCGGGCCGCGUCGACAGCCA 20 6748

HSV-UL54-1366 + CGUCUCAUCCGCGGACGCCA 20 16897

HSV-UL54-419 + CCCCGCUUGGGUGGCCAGCA 20 6842

HSV-UL54-1367 + CGUGGCGAGGUUUUCCAGCA 20 16898

HSV-UL54-1012 + GCGCAGCACGCAGUCGCGCA 20 7332

HSV-UL54-361 - UUACAUCCCGGGGGCCUGCA 20 6784

HSV-UL54-367 - AUCGCCCCCUUAUAUGUGCA 20 6790

HSV-UL54-322 - UGGGAAACCCUGGUCGCUCA 20 6745

HSV-UL54-1368 + GUCCGCGUGCGGGGGGGUCA 20 16899

HSV-UL54-1369 + GGGUCGAUGGUGUCGGCAGA 20 16900

HSV-UL54-414 + GG G U U U CCCAG G AAACCCG A 20 6837

HSV-UL54-1370 + CGCCCGAACGGCGGGGUCGA 20 16901

HSV-UL54-276 - CCCGCGUCGGCGCGGACGGA 20 6699

HSV-UL54-1371 + GGGACAGCGCCAUCAGCGGA 20 16902

HSV-UL54-294 - CUGACCCCCCCGCACGCGGA 20 6717

HSV-UL54-283 - ACGGCGAGGCGCGGCGCGGA 20 6706

HSV-UL54-275 - CCACCCCGCGUCGGCGCGGA 20 6698

HSV-UL54-337 - CUUUCUGCAGUGCUACCUGA 20 6760

HSV-UL54-366 - CAGUCGCGUGUGCGAGCUGA 20 6789

HSV-UL54-292 - CCAGGCCCCCCCUCCGCUGA 20 6715

HSV-UL54-1372 + GUUUAUUCUUGCCUAAAAUA 20 16903

HSV-UL54-1373 + GACCGCCGCGCGCUCGGAUA 20 16904

HSV-UL54-1374 + CGGCCGCCGUUCCGAUGAUA 20 16905

HSV-UL54-1375 + GUAUUUGCCGUGCACAUAUA 20 16906 HSV-UL54-344 - GCGACGCCUGUCGGACAUUA 20 6767

HSV-UL54-420 + CCCGCUUGGGUGGCCAGCAC 20 6843

HSV-UL54-392 + ACAGGCGUCGCCGCGAGCAC 20 6815

HSV-UL54-1376 + ACAGGUCGUCCAGCCCGCAC 20 16907

HSV-UL54-1377 + G G U U U CCCAGG AAACCCG AC 20 16908

HSV-UL54-1378 + CAAUAUCCUUAAUGUCCGAC 20 16909

HSV-UL54-1379 + CGUCAGCUCGCACACGCGAC 20 16910

HSV-UL54-1380 + GGCGCCUUUCGCUCCGGGAC 20 16911

HSV-UL54-1381 + UAAGGGGGCGAUAGUGUGAC 20 16912

HSV-UL54-364 - GGCGGGUCUCAUUGAAAUAC 20 6787

HSV-UL54-1382 + GGCUCGGGCCGUGAGCGACC 20 16913

HSV-UL54-1383 + GCGCCUUUCGCUCCGGGACC 20 16914

HSV-UL54-1028 + CAAAGGGGUCUUGCAUGACC 20 7341

HSV-UL54-321 - UCGGGUUUCCUGGGAAACCC 20 6744

HSV-UL54-332 - CAAUCUG CCG CUCCG CCCCC 20 6755

HSV-UL54-1384 + GACCCGCCAUGCAGGCCCCC 20 16915

HSV-UL54-308 - UUCCCGCCGCGAACAGCCCC 20 6731

HSV-UL54-1385 + AGACCCGCCAUGCAGGCCCC 20 16916

HSV-UL54-1386 + GCCUCGUGGCCGCGGGCCCC 20 16917

HSV-UL54-1387 + GGUGGCCAGCACGGGAGCCC 20 16918

HSV-UL54-295 - GCACGCGGACGGCCGCGCCC 20 6718

HSV-UL54-328 - GGAAAAUCUCAUCGAGGCCC 20 6751

HSV-UL54-277 - UCGGCGCGGACGGACGGCCC 20 6700

HSV-UL54-304 - AAGCUUUGGACGCAGUGCCC 20 6727

HSV-UL54-358 - ACGAUGCACUUUUACAUCCC 20 6781

HSV-UL54-1388 + GUGAGCGACCAGGGUUUCCC 20 16919

HSV-UL54-405 + GCGUCUCAUCCGCGGACGCC 20 6828

HSV-UL54-365 - AAUACUGGACACGCACCGCC 20 6788

HSV-UL54-291 - GCGGCCACGAGGCACGCGCC 20 6714

HSV-UL54-1389 + GCCUCGCCGUGGGUGGCGCC 20 16920

HSV-UL54-326 - GCGCGACUGCGUGCUGCGCC 20 6749

HSV-UL54-996 + CGCGGUUGGCGAGGCGGGCC 20 7319

HSV-UL54-1390 + CCAUCAGCGGAGGGGGGGCC 20 16921

HSV-UL54-357 - GACGAUGCACUUUUACAUCC 20 6780

HSV-UL54-346 - CUCCUUUGUGUUGGUCAUCC 20 6769

HSV-UL54-981 + ACACGCG ACU G G AACACU CC 20 7309

HSV-UL54-1391 + CAGAGGGCGCCUUUCGCUCC 20 16922

HSV-UL54-286 - GGGAGCAGCUCGACGUCUCC 20 6709

HSV-UL54-296 - GGACGGCCGCGCCCCGGUCC 20 6719

HSV-UL54-400 + CCGUUCCGAUGAUAGGGUCC 20 6823

HSV-UL54-319 - CCGAGACCCGUCGGGUUUCC 20 6742

HSV-UL54-407 + GGGCCUCGAUGAGAUUUUCC 20 6830

HSV-UL54-312 - CCCGUGCUGGCCACCCAAGC 20 6735

HSV-UL54-330 - GGCGUCCGCGGAUGAGACGC 20 6753

HSV-UL54-1392 + CCGUCCGUCCGCGCCGACGC 20 16923

HSV-UL54-324 - CACAUUCGCAGCCAACCCGC 20 6747 HSV-UL54-410 + UGGCUGUCGACGCGGCCCGC 20 6833

HSV-UL54-444 + GGCGCGUGCCUCGUGGCCGC 20 6867

HSV-UL54-1204 + GGGCGUU UCUGGAGACGCGC 20 16735

HSV-UL54-1393 + AGCACUGCAGAAAGGGGCGC 20 16924

HSV-UL54-455 + CGCCUCGCCGUGGGUGGCGC 20 6878

HSV-UL54-1394 + GUAAAAGUGCAUCGUCUCGC 20 16925

HSV-UL54-1395 + CUCGACGCGGUUGGCGAGGC 20 16926

HSV-UL54-1396 + CGCAGAACCGCCCGAACGGC 20 16927

HSV-UL54-1206 + GUU UCUGGAGACGCGCCGGC 20 16737

HSV-UL54-1397 + GCCCAGGGGCUGUUCGCGGC 20 16928

HSV-UL54-341 - GGCCCGAGGCCUGUGCGGGC 20 6764

HSV-UL54-356 - ACGACCGUGGGGGUUGGGGC 20 6779

HSV-UL54-363 - AUCCCGGGGGCCUGCAUGGC 20 6786

HSV-UL54-307 - UCAUGCAAGACCCCUUUGGC 20 6730

HSV-UL54-1398 + U U CAAU G AG ACCCG CCAU GC 20 16929

HSV-UL54-310 - CAGCCCCUGGGCUCCCGUGC 20 6733

HSV-UL54-336 - CGGAACGGCGGCCGCCGUGC 20 6759

HSV-UL54-440 + GGGCGCGGCCGUCCGCGUGC 20 6863

HSV-UL54-340 - UGAAGGCCCGAGGCCUGUGC 20 6763

HSV-UL54-1399 + GUCCAGCCCGCACAGGCCUC 20 16930

HSV-UL54-1400 + GCAGAGGGCGCCUUUCGCUC 20 16931

HSV-UL54-1401 + CGAAUGUGCGGUAGAGGCUC 20 16932

HSV-UL54-285 - GGGGAGCAGCUCGACGUCUC 20 6708

HSV-UL54-318 - GUUUGACGCCGAGACCCGUC 20 6741

HSV-UL54-1402 + CGUCCGCGUGCGGGGGGGUC 20 16933

HSV-UL54-1403 + CGCACAGGCCUCGGGCCUUC 20 16934

HSV-UL54-299 - CACCAUCGACCCCGCCGUUC 20 6722

HSV-UL54-1237 + CCCUUGGUUGUGGGCGUUUC 20 16768

HSV-UL54-1404 + GAAACGGCAUCCCGCCAAAG 20 16935

HSV-UL54-398 + UCAGGUAGCACUGCAGAAAG 20 6821

HSV-UL54-271 - CAG AAACG CCCACAACCA AG 20 6694

HSV-UL54-311 - UCCCGUGCUGGCCACCCAAG 20 6734

HSV-UL54-1405 + AUUUGCCGUGCACAUAUAAG 20 16936

HSV-UL54-1406 + UGGCCAGCACGGGAGCCCAG 20 16937

HSV-UL54-1407 + GGGGUCGAUGGUGUCGGCAG 20 16938

HSV-UL54-1408 + GUCAGGGACAGCGCCAUCAG 20 16939

HSV-UL54-1409 + GGACAGCGCCAUCAGCGGAG 20 16940

HSV-UL54-284 - CGGCGAGGCGCGGCGCGGAG 20 6707

HSV-UL54-1410 + AUAGGGUCCUGGGGGCGGAG 20 16941

HSV-UL54-1411 + UGGCUGCGAAUGUGCGGUAG 20 16942

HSV-UL54-290 - UCCGGGGGCCCGCGGCCACG 20 6713

HSV-UL54-293 - GUCCCUGACCCCCCCGCACG 20 6716

HSV-UL54-1412 + GCCGUCCGUCCGCGCCGACG 20 16943

HSV-UL54-1413 + CCGACACGCCGCGCUCGACG 20 16944

HSV-UL54-1414 + CAUGGCCUUGGCUGUCGACG 20 16945

HSV-UL54-349 - GGAGAUCGACUACACGACCG 20 6772 HSV-UL54-1415 + CGCCUUUCGCUCCGGGACCG 20 16946

HSV-UL54-323 - GCACAUUCGCAGCCAACCCG 20 6746

HSV-UL54-338 - CAGUGCUACCUGAAGGCCCG 20 6761

HSV-UL54-1416 + UUGGCUGUCGACGCGGCCCG 20 16947

HSV-UL54-289 - UCGACGUCUCCGGGGGCCCG 20 6712

HSV-UL54-359 - CGAUGCACUUUUACAUCCCG 20 6782

HSV-UL54-1417 + CCUCGCCGUGGGUGGCGCCG 20 16948

HSV-UL54-451 + CUCCGCGCCGCGCCUCGCCG 20 6874

HSV-UL54-1418 + UGGCGCGUGCCUCGUGGCCG 20 16949

HSV-UL54-1419 + CCACGCCAGCGUCUCAUCCG 20 16950

HSV-UL54-287 - GGAGCAGCUCGACGUCUCCG 20 6710

HSV-UL54-329 - CAUCGAGGCCCUGGCGUCCG 20 6752

HSV-UL54-313 - CCGUGCUGGCCACCCAAGCG 20 6736

HSV-UL54-347 - CUCGCCAACCGCGUCGAGCG 20 6770

HSV-UL54-1420 + CGUCCGUCCGCGCCGACGCG 20 16951

HSV-UL54-301 - GCGAUCCAUAUCCGAGCGCG 20 6724

HSV-UL54-1421 + CAGAAAGGGGCGCAGGCGCG 20 16952

HSV-UL54-280 - GCGCCACCCACGGCGAGGCG 20 6703

HSV-UL54-1422 + GCAGAACCGCCCGAACGGCG 20 16953

HSV-UL54-279 - CCCCGGCGCCACCCACGGCG 20 6702

HSV-UL54-1219 + UUUCUGGAGACGCGCCGGCG 20 16750

HSV-UL54-281 - ACCCACGGCGAGGCGCGGCG 20 6704

HSV-UL54-274 - GUCGCCACCCCGCGUCGGCG 20 6697

HSV-UL54-438 + UUCGCUCCGGGACCGGGGCG 20 6861

HSV-UL54-331 - CCGCGGAUGAGACGCUGGCG 20 6754

HSV-UL54-1423 + AGGGGCGCAGGCGCGUGGCG 20 16954

HSV-UL54-1424 + CGCGCUCGACGCGGUUGGCG 20 16955

HSV-UL54-1425 + GGCGCGGCCGUCCGCGUGCG 20 16956

HSV-UL54-327 - G CGCCAG G AAAAU CU CAU CG 20 6750

HSV-UL54-1426 + GGGGCCUGGCGCGUGCCUCG 20 16957

HSV-UL54-342 - GGCUGGACGACCUGUGCUCG 20 6765

HSV-UL54-1427 + GGGAGCCCAGGGGCUGUUCG 20 16958

HSV-UL54-1428 + CAGGAUGACCAACACAAAGG 20 16959

HSV-UL54-272 - AGAAACGCCCACAACCAAGG 20 6695

HSV-UL54-1429 + UUUGCCGUGCACAUAUAAGG 20 16960

HSV-UL54-1430 + GCUCGACGCGGUUGGCGAGG 20 16961

HSV-UL54-1431 + GACAGCGCCAUCAGCGGAGG 20 16962

HSV-UL54-1432 + UCGCAGAACCGCCCGAACGG 20 16963

HSV-UL54-335 - GGACCCUAUCAUCGGAACGG 20 6758

HSV-UL54-1433 + GGCGAGGUUUUCCAGCACGG 20 16964

HSV-UL54-360 - GAUGCACUUUUACAUCCCGG 20 6783

HSV-UL54-1220 + CGUUUCUGGAGACGCGCCGG 20 16751

HSV-UL54-288 - GAGCAGCUCGACGUCUCCGG 20 6711

HSV-UL54-314 - CGUGCUGGCCACCCAAGCGG 20 6737

HSV-UL54-1434 + AGGGACAGCGCCAUCAGCGG 20 16965

HSV-UL54-302 - AUCCAUAUCCGAGCGCGCGG 20 6725 HSV-UL54-282 - CACGGCGAGGCGCGGCGCGG 20 6705

HSV-UL54-1435 + AGCCCAGGGGCUGUUCGCGG 20 16966

HSV-UL54-1436 + GCGCGGCCGUCCGCGUGCGG 20 16967

HSV-UL54-1437 + ACAGCGCCAUCAGCGGAGGG 20 16968

HSV-UL54-315 - GUGCUGGCCACCCAAGCGGG 20 6738

HSV-UL54-1438 + CGCGGCCGUCCGCGUGCGGG 20 16969

HSV-UL54-300 - CAUCGACCCCGCCGUUCGGG 20 6723

HSV-UL54-1439 + CAGCGCCAUCAGCGGAGGGG 20 16970

HSV-UL54-316 - UGCUGGCCACCCAAGCGGGG 20 6739

HSV-UL54-1440 + CCGUCCGCGCCGACGCGGGG 20 16971

HSV-UL54-1441 + GCGGCCGUCCGCGUGCGGGG 20 16972

HSV-UL54-1442 + CGAUGAUAGGGUCCUGGGGG 20 16973

HSV-UL54-1443 + GCGCCGCGCCUCGCCGUGGG 20 16974

HSV-UL54-1444 + GUCAAACCCCCCCGCUUGGG 20 16975

HSV-UL54-362 - CAUCCCGGGGGCCUGCAUGG 20 6785

HSV-UL54-1445 + CGCGACUGGAACACUCCUGG 20 16976

HSV-UL54-401 + UUCCGAUGAUAGGGUCCUGG 20 6824

HSV-UL54-352 - GAUCGACUACACGACCGUGG 20 6775

HSV-UL54-306 - GUCAUGCAAGACCCCUUUGG 20 6729

HSV-UL54-1446 + GUUCCGAUGAUAGGGUCCUG 20 16977

HSV-UL54-351 - AGAUCGACUACACGACCGUG 20 6774

HSV-UL54-1447 + GGGGCGCGGCCGUCCGCGUG 20 16978

HSV-UL54-23 + GCGCCGGCGGGGUUUUGGUG 20 6446

HSV-UL54-411 + GCGGGUUGGCUGCGAAUGUG 20 6834

HSV-UL54-339 - CUGAAGGCCCGAGGCCUGUG 20 6762

HSV-UL54-403 + GGGGGCGGAGCGGCAGAUUG 20 6826

HSV-UL54-355 - CUACACGACCGUGGGGGUUG 20 6778

HSV-UL54-1448 + GGUGGCGACCCCCUUGGUUG 20 16979

HSV-UL54-1449 + UGUUUAUUCUUGCCUAAAAU 20 16980

HSV-UL54-333 - CGCCCCCAGGACCCUAUCAU 20 6756

HSV-UL54-1450 + GCGGCCGCCGUUCCGAUGAU 20 16981

HSV-UL54-462 + ACGCGGGGUGGCGACCCCCU 20 6885

HSV-UL54-309 - UCCCGCCGCGAACAGCCCCU 20 6732

HSV-UL54-1451 + CGUCCAGCCCGCACAGGCCU 20 16982

HSV-UL54-1452 + CACGCAGUCGCGCAUGGCCU 20 16983

HSV-UL54-1453 + CGUUCCGAUGAUAGGGUCCU 20 16984

HSV-UL54-320 - CGAGACCCGUCGGGUU UCCU 20 6743

HSV-UL54-1454 + CGGCGUCAAACCCCCCCGCU 20 16985

HSV-UL54-1455 + GCGCUCGACCGCCGCGCGCU 20 16986

HSV-UL54-1456 + GCGAAUGUGCGGUAGAGGCU 20 16987

HSV-UL54-1457 + CCAG G AAACCCG ACG G GU CU 20 16988

HSV-UL54-350 - GAGAUCGACUACACGACCGU 20 6773

HSV-UL54-317 - GGUUUGACGCCGAGACCCGU 20 6740

HSV-UL54-452 + UCCGCGCCGCGCCUCGCCGU 20 6875

HSV-UL54-273 - AGGGGGUCGCCACCCCGCGU 20 6696

HSV-UL54-995 + CACGCCGCGCUCGACGCGGU 20 7318 HSV-UL54-1458 + UGUCGACGCGGCCCGCGGGU 20 16989

HSV-UL54-353 - GACUACACGACCGUGGGGGU 20 6776

HSV-UL54-343 - GUGCUCGCGGCGACGCCUGU 20 6766

HSV-UL54-348 - CCGCGUCGAGCGCGGCGUGU 20 6771

HSV-UL54-437 + AACGGCGGGGUCGAUGGUGU 20 6860

HSV-UL54-345 - GGAUAUUGCCUCCUUUGUGU 20 6768

HSV-UL54-1459 + GUGGCGACCCCCUUGGUUGU 20 16990

HSV-UL54-305 - CUGGUCAUGCAAGACCCCUU 20 6728

HSV-UL54-303 - GAGCGCAUCAGCGAAAGCUU 20 6726

HSV-UL54-1460 + GGCGUCAAACCCCCCCGCUU 20 16991

HSV-UL54-298 - ACACCAUCGACCCCGCCGUU 20 6721

HSV-UL54-354 - ACUACACGACCGUGGGGGUU 20 6777

HSV-UL54-1207 + GAGACGCGCCGGCGGGGUUU 20 16738

HSV-UL54-1461 - CUACUGCAACUCCCUAUUUU 20 16992

Table 15A provides exemplary targeting domains for knocking out the UL54 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality, and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 15A

HSV-UL54-1474 + G CG ACC ACACG CCG G U G G U C 20 17005

HSV-UL54-1475 + GCGCGACCACACGCCGG UGGUC 22 17006

HSV-UL54-1476 + GGCGCGACCACACGCCGGUGGUC 23 17007

HSV-UL54-1477 + GCGAGGCGUCGAGGCU UC 18 17008

HSV-UL54-1478 + GGCGAGGCGUCGAGGCU UC 19 17009

HSV-UL54-169 + GGGCGAGGCGUCGAGGCUUC 20 6592

HSV-UL54-1479 + GCCGGGCGAGGCGUCGAGGCUUC 23 17010

HSV-UL54-1480 + GUUUCUGGAGACGCGCCGG 19 17011

HSV-UL54-1481 + GCGUUUCUGGAGACGCGCCGG 21 17012

HSV-UL54-1482 + GGCGUUUCUGGAGACGCGCCGG 22 17013

HSV-UL54-1483 + GGGCGUUUCUGGAGACGCGCCGG 23 17014

HSV-UL54-1484 + GCGGUGCCUUGGUCGACGGGGG 22 17015

HSV-UL54-1485 + GCCGGCGGGGUUU UGGUG 18 17016

HSV-UL54-23 + GCGCCGGCGGGGU U UUGGUG 20 6446

HSV-UL54-1486 + GACGCGCCGGCGGGGUU U UGGUG 23 17017

HSV-UL54-1487 + GCGGGCCGGGGGACCU UU 18 17018

HSV-UL54-1488 + GGCGGGCCGGGGGACCUUU 19 17019

HSV-UL54-45 + GGGCGGGCCGGGGGACCUU U 20 6468

HSV-UL54-1489 + GGGGCGGGCCGGGGGACCUU U 21 17020

HSV-UL54-1490 - GUCUCCAGAAACGCCCACAACCAA 24 17021

HSV-UL54-1491 - GAGGAGGGCCGCCGCGACGACC 22 17022

HSV-UL54-1492 - GACAUUGAUAUGCUAAUCGACC 22 17023

HSV-UL54-1493 - GCGGCGAGGUCGCCGCCGGGGC 22 17024

HSV-UL54-1494 - GGCGGCGAGGUCGCCGCCGGGGC 23 17025

HSV-UL54-1495 - GGGCGGCGAGGUCGCCGCCGGGGC 24 17026

HSV-UL54-1496 - GACCCCGAGUCCGACAGCAGCG 22 17027

Table 15B provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), and have a high level of orthogonality. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 15B

HSV-UL54-1499 + CGGGGAUGCGGUGCCUUGGUCGAC 24 17030

HSV-UL54-1500 + CCGCCCCUCCGUCUCCGC 18 17031

HSV-UL54-1501 + UCCGCCCCUCCGUCUCCGC 19 17032

HSV-UL54-180 + CUCCGCCCCUCCGUCUCCGC 20 6603

HSV-UL54-1502 + CCUCCGCCCCUCCGUCUCCGC 21 17033

HSV-UL54-1503 + UGGCCUCCGCCCCUCCGUCUCCGC 24 17034

HSV-UL54-1504 + UGUUGUAGGUCGCCGGGGC 19 17035

HSV-UL54-1236 + AUGUUGUAGGUCGCCGGGGC 20 16767

HSV-UL54-1505 + CAUGUUGUAGGUCGCCGGGGC 21 17036

HSV-UL54-1506 + CCAUGUUGUAGGUCGCCGGGGC 22 17037

HSV-UL54-1507 + AGCCAUGUUGUAGGUCGCCGGGGC 24 17038

HSV-UL54-1508 + CGACCUCGCCGCCCGCCUC 19 17039

HSV-UL54-1509 + CGGCGACCUCGCCGCCCGCCUC 22 17040

HSV-UL54-1510 + CGACCACACGCCGGUGGUC 19 17041

HSV-UL54-1511 + CGCGACCACACGCCGGUGGUC 21 17042

HSV-UL54-1512 + AGGCGCGACCACACGCCGGUGGUC 24 17043

HSV-UL54-1513 + CGGGCGAGGCGUCGAGGCUUC 21 17044

HSV-UL54-1514 + CCGGGCGAGGCGUCGAGGCUUC 22 17045

HSV-UL54-1515 + UGCCGGGCGAGGCGUCGAGGCUUC 24 17046

HSV-UL54-1516 + UUUCUGGAGACGCGCCGG 18 17047

HSV-UL54-1220 + CGUUUCUGGAGACGCGCCGG 20 16751

HSV-UL54-1517 + UGGGCGU UUCUGGAGACGCGCCGG 24 17048

HSV-UL54-1518 + CGGUGCCUUGGUCGACGGGGG 21 17049

HSV-UL54-1519 + UGCGGUGCCUUGGUCGACGGGGG 23 17050

HSV-UL54-1520 + AUGCGGUGCCUUGGUCGACGGGGG 24

17051

HSV-UL54-1521 + CGCCGGUGGUCGCGGGUG 18 17052

HSV-UL54-1522 + ACGCCGGUGGUCGCGGGUG 19 17053

HSV-UL54-1523 + CACGCCGGUGGUCGCGGGUG 20 17054

HSV-UL54-1524 + ACACGCCGGUGGUCGCGGGUG 21 17055

HSV-UL54-1525 + CACACGCCGGUGGUCGCGGGUG 22 17056

HSV-UL54-1526 + CCACACGCCGGUGGUCGCGGGUG 23 17057

HSV-UL54-1527 + ACCACACGCCGGUGGUCGCGGGUG 24 17058

HSV-UL54-1528 + CGCCGGCGGGGUUUUGGUG 19 17059

HSV-UL54-1529 + CGCGCCGGCGGGGUUUUGGUG 21 17060

HSV-UL54-1530 + ACGCGCCGGCGGGGUU UUGGUG 22 17061

HSV-UL54-1531 + AGACGCGCCGGCGGGGUUUUGGUG 24

17062

HSV-UL54-1532 + ACUCCCCGCUGCUGUCGGACU 21 17063

HSV-UL54-1533 + CACUCCCCGCUGCUGUCGGACU 22 17064

HSV-UL54-1534 + ACACUCCCCGCUGCUGUCGGACU 23 17065

HSV-UL54-1535 + AACACUCCCCGCUGCUGUCGGACU 24 17066

HSV-UL54-1536 + CGGGGCGGGCCGGGGGACCU UU 22 17067

HSV-UL54-1537 + UCGGGGCGGGCCGGGGGACCUUU 23 17068

HSV-UL54-1538 + CUCGGGGCGGGCCGGGGGACCUUU 24 17069

HSV-UL54-1539 - U CCAG AAACG CCCACAACCAA 21 17070 HSV-UL54-1540 - CUCCAGAAACGCCCACAACCAA 22 17071

HSV-UL54-1541 - U CU CCAG AAACGCCCACAACCAA 23 17072

HSV-UL54-1542 - AGGAGGGCCGCCGCGACGACC 21 17073

HSV-UL54-1543 - CGAGGAGGGCCGCCGCGACGACC 23 17074

HSV-UL54-1544 - ACGAGGAGGGCCGCCGCGACGACC 24 17075

HSV-UL54-1545 - UUGAUAUGCUAAUCGACC 18 17076

HSV-UL54-1546 - AUUGAUAUGCUAAUCGACC 19 17077

HSV-UL54-677 - CAUUGAUAUGCUAAUCGACC 20 7092

HSV-UL54-1547 - ACAUUGAUAUGCUAAUCGACC 21 17078

HSV-UL54-1548 - CGACAUUGAUAUGCUAAUCGACC 23 17079

HSV-UL54-1549 - CCGACAUUGAUAUGCUAAUCGACC 24 17080

HSV-UL54-1550 - CGGCGAGGUCGCCGCCGGGGC 21 17081

HSV-UL54-1551 - CCGAGUCCGACAGCAGCG 18 17082

HSV-UL54-1552 - CCCG AG U CCG ACAGCAG CG 19 17083

HSV-UL54-112 - CCCCGAGUCCGACAGCAGCG 20 6535

HSV-UL54-1553 - ACCCCGAGUCCGACAGCAGCG 21 17084

HSV-UL54-1554 - CGACCCCGAGUCCGACAGCAGCG 23 17085

HSV-UL54-1555 - ACGACCCCGAGUCCGACAGCAGCG 24 17086

Table 15C provides exemplary targeting domains for knocking out the UL54 gene selected according to the third tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 15C

HSV-UL54-693 - GGAGGGCCGCCGCGACGACC 20 7105

HSV-UL54-1565 - CG AGG UCGCCGCCGGGGC 18 17096

HSV-UL54-1566 - GCGAGGUCGCCGCCGGGGC 19 17097

HSV-UL54-143 - GGCG AGG UCGCCGCCGGGGC 20 6566

Table 15D provides exemplary targeting domains for knocking out the UL54 gene selected according to the fourth tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 15D

HSV-UL54-1590 + ACCUUCCCCCCGUGCGGUUCCC 22 17121

HSV-UL54-1591 + CACCUUCCCCCCGUGCGGUUCCC 23 17122

HSV-UL54-1592 + CCACCUUCCCCCCGUGCGGUUCCC 24 17123

HSV-UL54-1593 + GCGACCUCGCCGCCCGCC 18 17124

HSV-UL54-1594 + GGCGACCUCGCCGCCCGCC 19 17125

HSV-UL54-739 + CGGCGACCUCGCCGCCCGCC 20 7130

HSV-UL54-1595 + GCGGCGACCUCGCCGCCCGCC 21 17126

HSV-UL54-1596 + GGCGGCGACCUCGCCGCCCGCC 22 17127

HSV-UL54-1597 + CGGCGGCGACCUCGCCGCCCGCC 23 17128

HSV-UL54-1598 + CCGGCGGCGACCUCGCCGCCCGCC 24 17129

HSV-UL54-1599 + GCGUCCUCGGGGCGGGCC 18 17130

HSV-UL54-1600 + GGCGUCCUCGGGGCGGGCC 19 17131

HSV-UL54-176 + CGGCGUCCUCGGGGCGGGCC 20 6599

HSV-UL54-1601 + CCGGCGUCCUCGGGGCGGGCC 21 17132

HSV-UL54-1602 + GCCGGCGUCCUCGGGGCGGGCC 22 17133

HSV-UL54-1603 + UGCCGGCGUCCUCGGGGCGGGCC 23 17134

HSV-UL54-1604 + GUGCCGGCGUCCUCGGGGCGGGCC 24 17135

HSV-UL54-1605 + CGCUCCAGAGCGUCCUCC 18 17136

HSV-UL54-1606 + CCGCUCCAGAGCGUCCUCC 19 17137

HSV-UL54-779 + CCCGCUCCAGAGCGUCCUCC 20 7168

HSV-UL54-1607 + UCCCGCUCCAGAGCGUCCUCC 21 17138

HSV-UL54-1608 + GUCCCGCUCCAGAGCGUCCUCC 22 17139

HSV-UL54-1609 + CGUCCCGCUCCAGAGCGUCCUCC 23 17140

HSV-UL54-1610 + U CG U CCCG CUCCAG AG CG U CCU CC 24 17141

HSV-UL54-1611 + UCGGGGGUGCCGGCGUCC 18 17142

HSV-UL54-1612 + UUCGGGGGUGCCGGCGUCC 19 17143

HSV-UL54-1613 + CUUCGGGGGUGCCGGCGUCC 20 17144

HSV-UL54-1614 + GCUUCGGGGGUGCCGGCGUCC 21 17145

HSV-UL54-1615 + GGCUUCGGGGGUGCCGGCGUCC 22 17146

HSV-UL54-1616 + AGGCU UCGGGGGUGCCGGCGUCC 23 17147

HSV-UL54-1617 + GAGGCUUCGGGGGUGCCGGCGUCC 24 17148

HSV-UL54-1618 + UUCCCCCCGUGCGGUUCC 18 17149

HSV-UL54-1619 + CUUCCCCCCGUGCGGUUCC 19 17150

HSV-UL54-158 + CCUUCCCCCCGUGCGGUUCC 20 6581

HSV-UL54-1620 + ACCUUCCCCCCGUGCGGUUCC 21 17151

HSV-UL54-1621 + CACCUUCCCCCCGUGCGGUUCC 22 17152

HSV-UL54-1622 + CCACCUUCCCCCCGUGCGGUUCC 23 17153

HSV-UL54-1623 + GCCACCUUCCCCCCGUGCGGUUCC 24 17154

HSV-UL54-1624 + GUGUGGAGUCGGCGCCGC 18 17155

HSV-UL54-1625 + GGUGUGGAGUCGGCGCCGC 19 17156

HSV-UL54-149 + UGGUGUGGAGUCGGCGCCGC 20 6572

HSV-UL54-1626 + UUGGUGUGGAGUCGGCGCCGC 21 17157

HSV-UL54-1627 + UUUGGUGUGGAGUCGGCGCCGC 22 17158

HSV-UL54-1628 + UUUUGGUGUGGAGUCGGCGCCGC 23 17159

HSV-UL54-1629 + GUUUUGGUGUGGAGUCGGCGCCGC 24

17160 HSV-UL54-1630 + AGCCAUGUUGUAGGUCGC 18 17161

HSV-UL54-1631 + UAGCCAUGUUGUAGGUCGC 19 17162

HSV-UL54-1205 + GUAGCCAUGUUGUAGGUCGC 20 16736

HSV-UL54-1632 + GG UAGCCAUGUUGUAGGUCGC 21 17163

HSV-UL54-1633 + CGGUAGCCAUGUUGUAGGUCGC 22 17164

HSV-UL54-1634 + UCGGUAGCCAUGUUGUAGGUCGC 23 17165

HSV-UL54-1635 + GUCGGUAGCCAUGUUGUAGGUCGC 24 17166

HSV-UL54-1636 + GGCGUCCUCGGGGCGGGC 18 17167

HSV-UL54-1637 + CGGCGUCCUCGGGGCGGGC 19 17168

HSV-UL54-175 + CCGGCGUCCUCGGGGCGGGC 20 6598

HSV-UL54-1638 + GCCGGCGUCCUCGGGGCGGGC 21 17169

HSV-UL54-1639 + UGCCGGCGUCCUCGGGGCGGGC 22 17170

HSV-UL54-1640 + GUGCCGGCGUCCUCGGGGCGGGC 23 17171

HSV-UL54-1641 + GGUGCCGGCGUCCUCGGGGCGGGC 24 17172

HSV-UL54-1642 + GCCCUCCUCGUCCCGCUC 18 17173

HSV-UL54-1643 + GGCCCUCCUCGUCCCGCUC 19 17174

HSV-UL54-1644 + CGGCCCUCCUCGUCCCGCUC 20 17175

HSV-UL54-1645 + GCGGCCCUCCUCGUCCCGCUC 21 17176

HSV-UL54-1646 + GGCGGCCCUCCUCGUCCCGCUC 22 17177

HSV-UL54-1647 + CGGCGGCCCUCCUCGUCCCGCUC 23 17178

HSV-UL54-1648 + GCGGCGGCCCUCCUCGUCCCGCUC 24 17179

HSV-UL54-1649 + GAAGCCGACCGCCUGGUC 18 17180

HSV-UL54-1650 + GGAAGCCGACCGCCUGGUC 19 17181

HSV-UL54-1651 + GGGAAGCCGACCGCCUGGUC 20 17182

HSV-UL54-1652 + GGGGAAGCCGACCGCCUGGUC 21 17183

HSV-UL54-1653 + GGGGGAAGCCGACCGCCUGGUC 22 17184

HSV-UL54-1654 + GGGGGGAAGCCGACCGCCUGGUC 23 17185

HSV-UL54-1655 + CGGGGGGAAGCCGACCGCCUGGUC 24 17186

HSV-UL54-1656 + CUUCCCCCCGUGCGGUUC 18 17187

HSV-UL54-1657 + CCUUCCCCCCGUGCGGUUC 19 17188

HSV-UL54-1658 + ACCUUCCCCCCGUGCGGUUC 20 17189

HSV-UL54-1659 + CACCUUCCCCCCGUGCGGUUC 21 17190

HSV-UL54-1660 + CCACCUUCCCCCCGUGCGGUUC 22 17191

HSV-UL54-1661 + GCCACCUUCCCCCCGUGCGGUUC 23 17192

HSV-UL54-1662 + GGCCACCUUCCCCCCGUGCGGUUC 24 17193

HSV-UL54-1663 + CUUGGUUGUGGGCGUUUC 18 17194

HSV-UL54-1664 + CCUUGGUUGUGGGCGUUUC 19 17195

HSV-UL54-1237 + CCCUUGGUUGUGGGCGUUUC 20 16768

HSV-UL54-1665 + CCCCUUGGUUGUGGGCGUUUC 21 17196

HSV-UL54-1666 + CCCCCUUGGUUGUGGGCGUUUC 22 17197

HSV-UL54-1667 + ACCCCCUUGGUUGUGGGCGUUUC 23 17198

HSV-UL54-1668 + GACCCCCUUGGUUGUGGGCGUUUC 24 17199

HSV-UL54-1669 + CCAUGUCCUCGUCCGACG 18 17200

HSV-UL54-1670 + UCCAUGUCCUCGUCCGACG 19 17201

HSV-UL54-47 + UUCCAUGUCCUCGUCCGACG 20 6470

HSV-UL54-1671 + CUUCCAUGUCCUCGUCCGACG 21 17202 HSV-UL54-1672 + UCUUCCAUGUCCUCGUCCGACG 22 17203

HSV-UL54-1673 + GUCUUCCAUGUCCUCGUCCGACG 23 17204

HSV-UL54-1674 + GGUCUUCCAUGUCCUCGUCCGACG 24 17205

HSV-UL54-1675 + CCCCCCGUGCGGUUCCCG 18 17206

HSV-UL54-1676 + U CCCCCCG U GCG G U U CCCG 19 17207

HSV-UL54-160 + UUCCCCCCGUGCGGUUCCCG 20 6583

HSV-UL54-1677 + CUUCCCCCCGUGCGGUUCCCG 21 17208

HSV-UL54-1678 + CCUUCCCCCCGUGCGGUUCCCG 22 17209

HSV-UL54-1679 + ACCUUCCCCCCGUGCGGUUCCCG 23 17210

HSV-UL54-1680 + CACCUUCCCCCCGUGCGGUUCCCG 24 17211

HSV-UL54-1681 + GUUUCUGGAGACGCGCCG 18 17212

HSV-UL54-1682 + CGUUUCUGGAGACGCGCCG 19 17213

HSV-UL54-1683 + GCGUU UCUGGAGACGCGCCG 20 17214

HSV-UL54-1684 + GGCGUUUCUGGAGACGCGCCG 21 17215

HSV-UL54-1685 + GGGCGUU UCUGGAGACGCGCCG 22 17216

HSV-UL54-1686 + UGGGCGUUUCUGGAGACGCGCCG 23 17217

HSV-UL54-1687 + GUGGGCGUUUCUGGAGACGCGCCG 24 17218

HSV-UL54-1688 + GGUGUGGAGUCGGCGCCG 18 17219

HSV-UL54-1689 + UGGUGUGGAGUCGGCGCCG 19 17220

HSV-UL54-1690 + UUGGUGUGGAGUCGGCGCCG 20 17221

HSV-UL54-1691 + UU UGGUGUGGAGUCGGCGCCG 21 17222

HSV-UL54-1692 + UUUUGGUGUGGAGUCGGCGCCG 22 17223

HSV-UL54-1693 + GUU UUGGUGUGGAGUCGGCGCCG 23 17224

HSV-UL54-1694 + GGUUUUGGUGUGGAGUCGGCGCCG 24

17225

HSV-UL54-1695 + CGUCCUCGGGGCGGGCCG 18 17226

HSV-UL54-1696 + GCGUCCUCGGGGCGGGCCG 19 17227

HSV-UL54-177 + GGCGUCCUCGGGGCGGGCCG 20 6600

HSV-UL54-1697 + CGGCGUCCUCGGGGCGGGCCG 21 17228

HSV-UL54-1698 + CCGGCGUCCUCGGGGCGGGCCG 22 17229

HSV-UL54-1699 + GCCGGCGUCCUCGGGGCGGGCCG 23 17230

HSV-UL54-1700 + UGCCGGCGUCCUCGGGGCGGGCCG 24 17231

HSV-UL54-1701 + UCCGCCCCUCCGUCUCCG 18 17232

HSV-UL54-1702 + CUCCGCCCCUCCGUCUCCG 19 17233

HSV-UL54-1703 + CCUCCGCCCCUCCGUCUCCG 20 17234

HSV-UL54-1704 + GCCUCCGCCCCUCCGUCUCCG 21 17235

HSV-UL54-1705 + GGCCUCCGCCCCUCCGUCUCCG 22 17236

HSV-UL54-1706 + UGGCCUCCGCCCCUCCGUCUCCG 23 17237

HSV-UL54-1707 + AUGGCCUCCGCCCCUCCGUCUCCG 24 17238

HSV-UL54-1708 + CUUCCAUGUCCUCGUCCG 18 17239

HSV-UL54-1709 + UCUUCCAUGUCCUCGUCCG 19 17240

HSV-UL54-1710 + GUCUUCCAUGUCCUCGUCCG 20 17241

HSV-UL54-1711 + GGUCUUCCAUGUCCUCGUCCG 21 17242

HSV-UL54-1712 + GGGUCU UCCAUGUCCUCGUCCG 22 17243

HSV-UL54-1713 + GGGGUCUUCCAUGUCCUCGUCCG 23 17244

HSV-UL54-1714 + AGGGGUCUUCCAUGUCCUCGUCCG 24 17245 HSV-UL54-1715 + GCGCUGCCGGGCGAGGCG 18 17246

HSV-UL54-1716 + CGCGCUGCCGGGCGAGGCG 19 17247

HSV-UL54-753 + CCGCGCUGCCGGGCGAGGCG 20 7144

HSV-UL54-1717 + GCCGCGCUGCCGGGCGAGGCG 21 17248

HSV-UL54-1718 + CGCCGCGCUGCCGGGCGAGGCG 22 17249

HSV-UL54-1719 + CCGCCGCGCUGCCGGGCGAGGCG 23 17250

HSV-UL54-1720 + CCCGCCGCGCUGCCGGGCGAGGCG 24 17251

HSV-UL54-1721 + UAGCCAUGUUGUAGGUCG 18 17252

HSV-UL54-1722 + GUAGCCAUGUUGUAGGUCG 19 17253

HSV-UL54-1723 + GGUAGCCAUGUUGUAGGUCG 20 17254

HSV-UL54-1724 + CGG UAGCCAUGUUGUAGGUCG 21 17255

HSV-UL54-1725 + UCGGUAGCCAUGUUGUAGGUCG 22 17256

HSV-UL54-1726 + GUCGGUAGCCAUGUUGUAGGUCG 23 17257

HSV-UL54-1727 + UGUCGGUAGCCAUGUUGUAGGUCG 24

17258

HSV-UL54-1728 + GAUGCGGUGCCUUGGUCG 18 17259

HSV-UL54-1729 + GGAUGCGGUGCCUUGGUCG 19 17260

HSV-UL54-1730 + GGGAUGCGGUGCCUUGGUCG 20 17261

HSV-UL54-1731 + GGGGAUGCGGUGCCUUGGUCG 21 17262

HSV-UL54-1732 + CGGGGAUGCGGUGCCUUGGUCG 22 17263

HSV-UL54-1733 + UCGGGGAUGCGGUGCCUUGGUCG 23 17264

HSV-UL54-1734 + CUCGGGGAUGCGGUGCCUUGGUCG 24 17265

HSV-UL54-1735 + CCCCCGUGCGGUUCCCGG 18 17266

HSV-UL54-1736 + CCCCCCGUGCGGUUCCCGG 19 17267

HSV-UL54-34 + UCCCCCCGUGCGGUUCCCGG 20 6457

HSV-UL54-1737 + U U CCCCCCG UGCGGU UCCCGG 21 17268

HSV-UL54-1738 + CUUCCCCCCGUGCGGUUCCCGG 22 17269

HSV-UL54-1739 + CCUUCCCCCCGUGCGGUUCCCGG 23 17270

HSV-UL54-1740 + ACCUUCCCCCCGUGCGGUUCCCGG 24 17271

HSV-UL54-1741 + UCCCCGCCGCGCUGCCGG 18 17272

HSV-UL54-1742 + CUCCCCGCCGCGCUGCCGG 19 17273

HSV-UL54-1743 + GCUCCCCGCCGCGCUGCCGG 20 17274

HSV-UL54-1744 + GGCUCCCCGCCGCGCUGCCGG 21 17275

HSV-UL54-1745 + CGGCUCCCCGCCGCGCUGCCGG 22 17276

HSV-UL54-1746 + UCGGCUCCCCGCCGCGCUGCCGG 23 17277

HSV-UL54-1747 + GUCGGCUCCCCGCCGCGCUGCCGG 24 17278

HSV-UL54-1748 + CCCCGUGCGGUUCCCGGG 18 17279

HSV-UL54-1749 + CCCCCGUGCGGUUCCCGGG 19 17280

HSV-UL54-35 + CCCCCCGUGCGGUUCCCGGG 20 6458

HSV-UL54-1750 + UCCCCCCGUGCGGUUCCCGGG 21 17281

HSV-UL54-1751 + UUCCCCCCGUGCGGUUCCCGGG 22 17282

HSV-UL54-1752 + CUUCCCCCCGUGCGGUUCCCGGG 23 17283

HSV-UL54-1753 + CCUUCCCCCCGUGCGGUUCCCGGG 24 17284

HSV-UL54-1754 + CGGCGUCCUCGGGGCGGG 18 17285

HSV-UL54-1755 + CCGGCGUCCUCGGGGCGGG 19 17286

HSV-UL54-1756 + GCCGGCGUCCUCGGGGCGGG 20 17287 HSV-UL54-1757 + UGCCGGCGUCCUCGGGGCGGG 21 17288

HSV-UL54-1758 + GUGCCGGCGUCCUCGGGGCGGG 22 17289

HSV-UL54-1759 + GGUGCCGGCGUCCUCGGGGCGGG 23 17290

HSV-UL54-1760 + GGGUGCCGGCGUCCUCGGGGCGGG 24 17291

HSV-UL54-1761 + GGUGCCGGCGUCCUCGGG 18 17292

HSV-UL54-1762 + GGGUGCCGGCGUCCUCGGG 19 17293

HSV-UL54-1763 + GGGGUGCCGGCGUCCUCGGG 20 17294

HSV-UL54-1764 + GGGGGUGCCGGCGUCCUCGGG 21 17295

HSV-UL54-1765 + CGGGGGUGCCGGCGUCCUCGGG 22 17296

HSV-UL54-1766 + UCGGGGGUGCCGGCGUCCUCGGG 23 17297

HSV-UL54-1767 + UUCGGGGGUGCCGGCGUCCUCGGG 24 17298

HSV-UL54-1768 + UGUUGUAGGUCGCCGGGG 18 17299

HSV-UL54-1769 + AUGUUGUAGGUCGCCGGGG 19 17300

HSV-UL54-1770 + CAUGUUGUAGGUCGCCGGGG 20 17301

HSV-UL54-1771 + CCAUGUUGUAGGUCGCCGGGG 21 17302

HSV-UL54-1772 + GCCAUGU UGUAGGUCGCCGGGG 22 17303

HSV-UL54-1773 + AGCCAUGUUGUAGGUCGCCGGGG 23 17304

HSV-UL54-1774 + UAGCCAUGUUGUAGGUCGCCGGGG 24 17305

HSV-UL54-1775 + GUCGCCGGGGCUGGGAUG 18 17306

HSV-UL54-1776 + GGUCGCCGGGGCUGGGAUG 19 17307

HSV-UL54-1777 + AGGUCGCCGGGGCUGGGAUG 20 17308

HSV-UL54-1778 + UAGGUCGCCGGGGCUGGGAUG 21 17309

HSV-UL54-1779 + GU AGGUCGCCGGGGCUGGGAUG 22 17310

HSV-UL54-1780 + UGU AGGUCGCCGGGGCUGGGAUG 23 17311

HSV-UL54-1781 + UUGUAGGUCGCCGGGGCUGGGAUG 24

17312

HSV-UL54-1782 + CGGCUCCCCGCCGCGCUG 18 17313

HSV-UL54-1783 + UCGGCUCCCCGCCGCGCUG 19 17314

HSV-UL54-1784 + GUCGGCUCCCCGCCGCGCUG 20 17315

HSV-UL54-1785 + CGUCGGCUCCCCGCCGCGCUG 21 17316

HSV-UL54-1786 + UCGUCGGCUCCCCGCCGCGCUG 22 17317

HSV-UL54-1787 + AUCGUCGGCUCCCCGCCGCGCUG 23 17318

HSV-UL54-1788 + GAUCGUCGGCUCCCCGCCGCGCUG 24 17319

HSV-UL54-1789 + UCCUCCUCGAGCUCGCUG 18 17320

HSV-UL54-1790 + GUCCUCCUCGAGCUCGCUG 19 17321

HSV-UL54-1791 + CGUCCUCCUCGAGCUCGCUG 20 17322

HSV-UL54-1792 + GCGUCCUCCUCGAGCUCGCUG 21 17323

HSV-UL54-1793 + AGCGUCCUCCUCGAGCUCGCUG 22 17324

HSV-UL54-1794 + GAGCGUCCUCCUCGAGCUCGCUG 23 17325

HSV-UL54-1795 + AGAGCGUCCUCCUCGAGCUCGCUG 24 17326

HSV-UL54-1796 + GAACACUCCCCGCUGCUG 18 17327

HSV-UL54-1797 + GGAACACUCCCCGCUGCUG 19 17328

HSV-UL54-1798 + AGGAACACUCCCCGCUGCUG 20 17329

HSV-UL54-1799 + GAGGAACACUCCCCGCUGCUG 21 17330

HSV-UL54-1800 + CGAGGAACACUCCCCGCUGCUG 22 17331

HSV-UL54-1801 + ACGAGGAACACUCCCCGCUGCUG 23 17332 HSV-UL54-1802 + GACGAGGAACACUCCCCGCUGCUG 24 17333

HSV-UL54-1803 + GGUCGACGGGGGUUGGAU 18 17334

HSV-UL54-1804 + UGGUCGACGGGGGUUGGAU 19 17335

HSV-UL54-1805 + UU GGUCGACGGGGGUUGGAU 20 17336

HSV-UL54-1806 + CU UGGUCGACGGGGGUUGGAU 21 17337

HSV-UL54-1807 + CCUUGGUCGACGGGGGUUGGAU 22 17338

HSV-UL54-1808 + GCCU UGGUCGACGGGGGUUGGAU 23 17339

HSV-UL54-1809 + UGCCUUGGUCGACGGGGGUUGGAU 24

17340

HSV-UL54-1810 + GGGCGGGCCGGGGGACCU 18 17341

HSV-UL54-1811 + GGGGCGGGCCGGGGGACCU 19 17342

HSV-UL54-1812 + CGGGGCGGGCCGGGGGACCU 20 17343

HSV-UL54-1813 + UCGGGGCGGGCCGGGGGACCU 21 17344

HSV-UL54-1814 + CUCGGGGCGGGCCGGGGGACCU 22 17345

HSV-UL54-1815 + CCUCGGGGCGGGCCGGGGGACCU 23 17346

HSV-UL54-1816 + UCCUCGGGGCGGGCCGGGGGACCU 24 17347

HSV-UL54-1817 + CGACCUCGCCGCCCGCCU 18 17348

HSV-UL54-1818 + GCGACCUCGCCGCCCGCCU 19 17349

HSV-UL54-152 + GGCGACCUCGCCGCCCGCCU 20 6575

HSV-UL54-1819 + CGGCGACCUCGCCGCCCGCCU 21 17350

HSV-UL54-1820 + GCGGCGACCUCGCCGCCCGCCU 22 17351

HSV-UL54-1821 + GGCGGCGACCUCGCCGCCCGCCU 23 17352

HSV-UL54-1822 + CGGCGGCGACCUCGCCGCCCGCCU 24 17353

HSV-UL54-1823 + CGGGGGUGCCGGCGUCCU 18 17354

HSV-UL54-1824 + UCGGGGGUGCCGGCGUCCU 19 17355

HSV-UL54-171 + UUCGGGGGUGCCGGCGUCCU 20 6594

HSV-UL54-1825 + CUUCGGGGGUGCCGGCGUCCU 21 17356

HSV-UL54-1826 + GCUUCGGGGGUGCCGGCGUCCU 22 17357

HSV-UL54-1827 + GGCUUCGGGGGUGCCGGCGUCCU 23 17358

HSV-UL54-1828 + AGGCUUCGGGGGUGCCGGCGUCCU 24 17359

HSV-UL54-1829 + GGGCGAGGCGUCGAGGCU 18 17360

HSV-UL54-1830 + CGGGCGAGGCGUCGAGGCU 19 17361

HSV-UL54-1831 + CCGGGCGAGGCGUCGAGGCU 20 17362

HSV-UL54-1832 + GCCGGGCGAGGCGUCGAGGCU 21 17363

HSV-UL54-1833 + UGCCGGGCGAGGCGUCGAGGCU 22 17364

HSV-UL54-1834 + CUGCCGGGCGAGGCGUCGAGGCU 23 17365

HSV-UL54-1835 + GCUGCCGGGCGAGGCGUCGAGGCU 24 17366

HSV-UL54-1836 + CGCCGGCGGGGUUU UGGU 18 17367

HSV-UL54-1837 + GCGCCGGCGGGGUUUUGGU 19 17368

HSV-UL54-1838 + CGCGCCGGCGGGGUUUUGGU 20 17369

HSV-UL54-1839 + ACGCGCCGGCGGGGUUUUGGU 21 17370

HSV-UL54-1840 + GACGCGCCGGCGGGGUUUUGGU 22 17371

HSV-UL54-1841 + AGACGCGCCGGCGGGGUUUUGGU 23 17372

HSV-UL54-1842 + GAGACGCGCCGGCGGGGUUUUGGU 24

17373

HSV-UL54-1843 + GGCGGGCCGGGGGACCUU 18 17374 HSV-UL54-1844 + GGGCGGGCCGGGGGACCUU 19 17375

HSV-UL54-179 + GGGGCGGGCCGGGGGACCU U 20 6602

HSV-UL54-1845 + CGGGGCGGGCCGGGGGACCUU 21 17376

HSV-UL54-1846 + UCGGGGCGGGCCGGGGGACCUU 22 17377

HSV-UL54-1847 + CUCGGGGCGGGCCGGGGGACCU U 23 17378

HSV-UL54-1848 + CCUCGGGGCGGGCCGGGGGACCUU 24 17379

HSV-UL54-1849 + GGCGAGGCGUCGAGGCUU 18 17380

HSV-UL54-1850 + GGGCGAGGCGUCGAGGCUU 19 17381

HSV-UL54-41 + CGGGCGAGGCGUCGAGGCUU 20 6464

HSV-UL54-1851 + CCGGGCGAGGCGUCGAGGCUU 21 17382

HSV-UL54-1852 + GCCGGGCGAGGCGUCGAGGCUU 22 17383

HSV-UL54-1853 + UGCCGGGCGAGGCGUCGAGGCUU 23 17384

HSV-UL54-1854 + CUGCCGGGCGAGGCGUCGAGGCUU 24 17385

HSV-UL54-1855 + CCUUGGU UGUGGGCGUUU 18 17386

HSV-UL54-1856 + CCCUUGGUUGUGGGCGUUU 19 17387

HSV-UL54-1857 + CCCCUUGGUUGUGGGCGUUU 20 17388

HSV-UL54-1858 + CCCCCUUGGUUGUGGGCGUUU 21 17389

HSV-UL54-1859 + ACCCCCUUGGUUGUGGGCGUUU 22 17390

HSV-UL54-1860 + GACCCCCUUGGUUGUGGGCGUUU 23 17391

HSV-UL54-1861 + CGACCCCCUUGGUUGUGGGCGUUU 24 17392

HSV-UL54-1862 - GAUUGGACCUGUCCGACA 18 17393

HSV-UL54-1863 - GGAUUGGACCUGUCCGACA 19 17394

HSV-UL54-686 - AGGAUUGGACCUGUCCGACA 20 7099

HSV-UL54-1864 - UAGGAUUGGACCUGUCCGACA 21 17395

HSV-UL54-1865 - CUAGGAUUGGACCUGUCCGACA 22 17396

HSV-UL54-1866 - CCUAGGAUUGGACCUGUCCGACA 23 17397

HSV-UL54-1867 - ACCUAGGAUUGGACCUGUCCGACA 24 17398

HSV-UL54-1868 - CCUCGUCGGACGAGGACA 18 17399

HSV-UL54-1869 - UCCUCGUCGGACGAGGACA 19 17400

HSV-UL54-6 - UUCCUCGUCGGACGAGGACA 20 6429

HSV-UL54-1870 - GU UCCUCGUCGGACGAGGACA 21 17401

HSV-UL54-1871 - UGUUCCUCGUCGGACGAGGACA 22 17402

HSV-UL54-1872 - GUGU UCCUCGUCGGACGAGGACA 23 17403

HSV-UL54-1873 - AGUGUUCCUCGUCGGACGAGGACA 24 17404

HSV-UL54-1874 - CAGAAACG CCCACAACCA 18 17405

HSV-UL54-1875 - CCAG AAACG CCCACAACCA 19 17406

HSV-UL54-1216 - UCCAGAAACGCCCACAACCA 20 16747

HSV-UL54-1876 - CUCCAGAAACGCCCACAACCA 21 17407

HSV-UL54-1877 - UCUCCAGAAACGCCCACAACCA 22 17408

HSV-UL54-1878 - G U CU CCAG AAACGCCCACAACCA 23 17409

HSV-UL54-1879 - CG U CU CCAG AAACGCCCACAACCA 24 17410

HSV-UL54-1880 - ACCCCGAGUCCGACAGCA 18 17411

HSV-UL54-1881 - GACCCCGAGUCCGACAGCA 19 17412

HSV-UL54-694 - CGACCCCGAGUCCGACAGCA 20 7106

HSV-UL54-1882 - ACGACCCCGAGUCCGACAGCA 21 17413

HSV-UL54-1883 - GACGACCCCGAGUCCGACAGCA 22 17414 HSV-UL54-1884 - CG ACGACCCCGAG UCCGACAGCA 23 17415

HSV-UL54-1885 - GCGACGACCCCGAGUCCGACAGCA 24 17416

HSV-UL54-1886 - UUCCCCCCGGGAACCGCA 18 17417

HSV-UL54-1887 - CUUCCCCCCGGGAACCGCA 19 17418

HSV-UL54-16 - GCUUCCCCCCGGGAACCGCA 20 6439

HSV-UL54-1888 - GGCUUCCCCCCGGGAACCGCA 21 17419

HSV-UL54-1889 - CGGCUUCCCCCCGGGAACCGCA 22 17420

HSV-UL54-1890 - UCGGCUUCCCCCCGGGAACCGCA 23 17421

HSV-UL54-1891 - GUCGGCUUCCCCCCGGGAACCGCA 24 17422

HSV-UL54-1892 - GGAAGACCCCUGCGGAGA 18 17423

HSV-UL54-1893 - UGGAAGACCCCUGCGGAGA 19 17424

HSV-UL54-116 - AUGGAAGACCCCUGCGGAGA 20 6539

HSV-UL54-1894 - CAUGGAAGACCCCUGCGGAGA 21 17425

HSV-UL54-1895 - ACAUGGAAGACCCCUGCGGAGA 22 17426

HSV-UL54-1896 - GACAUGGAAGACCCCUGCGGAGA 23 17427

HSV-UL54-1897 - GGACAUGGAAGACCCCUGCGGAGA 24 17428

HSV-UL54-1898 - AUGCUAAUCGACCUAGGA 18 17429

HSV-UL54-1899 - UAUGCUAAUCGACCUAGGA 19 17430

HSV-UL54-685 - AU AUGCUAAUCGACCUAGGA 20 7098

HSV-UL54-1900 - GAU AUGCUAAUCGACCUAGGA 21 17431

HSV-UL54-1901 - UGAUAUGCUAAUCGACCUAGGA 22 17432

HSV-UL54-1902 - UUGAU AUGCUAAUCGACCUAGGA 23 17433

HSV-UL54-1903 - AUUGAUAUGCUAAUCGACCUAGGA 24 17434

HSV-UL54-1904 - CGAGGAGGACGCUCUGGA 18 17435

HSV-UL54-1905 - UCGAGGAGGACGCUCUGGA 19 17436

HSV-UL54-690 - CUCGAGGAGGACGCUCUGGA 20 7102

HSV-UL54-1906 - GCUCGAGGAGGACGCUCUGGA 21 17437

HSV-UL54-1907 - AGCUCGAGGAGGACGCUCUGGA 22 17438

HSV-UL54-1908 - GAGCUCGAGGAGGACGCUCUGGA 23 17439

HSV-UL54-1909 - CGAGCUCGAGGAGGACGCUCUGGA 24 17440

HSV-UL54-1910 - UCCCCCCGGGAACCGCAC 18 17441

HSV-UL54-1911 - UUCCCCCCGGGAACCGCAC 19 17442

HSV-UL54-132 - CUUCCCCCCGGGAACCGCAC 20 6555

HSV-UL54-1912 - GCUUCCCCCCGGGAACCGCAC 21 17443

HSV-UL54-1913 - GGCUUCCCCCCGGGAACCGCAC 22 17444

HSV-UL54-1914 - CGGCUUCCCCCCGGGAACCGCAC 23 17445

HSV-UL54-1915 - UCGGCUUCCCCCCGGGAACCGCAC 24 17446

HSV-UL54-1916 - UCCUCGUCGGACGAGGAC 18 17447

HSV-UL54-1917 - UUCCUCGUCGGACGAGGAC 19 17448

HSV-UL54-700 - GUUCCUCGUCGGACGAGGAC 20 7110

HSV-UL54-1918 - UGU UCCUCGUCGGACGAGGAC 21 17449

HSV-UL54-1919 - GUGUUCCUCGUCGGACGAGGAC 22 17450

HSV-UL54-1920 - AG UGUUCCUCGUCGGACGAGGAC 23 17451

HSV-UL54-1921 - GAGUGUUCCUCGUCGGACGAGGAC 24 17452

HSV-UL54-1922 - GAGUGUUCCUCGUCGGAC 18 17453

HSV-UL54-1923 - GGAGUGUUCCUCGUCGGAC 19 17454 HSV-UL54-699 - GGGAGUGUUCCUCGUCGGAC 20 7109

HSV-UL54-1924 - GGGGAGUGUUCCUCGUCGGAC 21 17455

HSV-UL54-1925 - CGGGGAGUGUUCCUCGUCGGAC 22 17456

HSV-UL54-1926 - GCGGGGAGUGUUCCUCGUCGGAC 23 17457

HSV-UL54-1927 - AGCGGGGAGUGUUCCUCGUCGGAC 24 17458

HSV-UL54-1928 - GACGCUCUGGAGCGGGAC 18 17459

HSV-UL54-1929 - GGACGCUCUGGAGCGGGAC 19 17460

HSV-UL54-683 - AGGACGCUCUGGAGCGGGAC 20 7097

HSV-UL54-1930 - GAGGACGCUCUGGAGCGGGAC 21 17461

HSV-UL54-1931 - GGAGGACGCUCUGGAGCGGGAC 22 17462

HSV-UL54-1932 - AGGAGGACGCUCUGGAGCGGGAC 23 17463

HSV-UL54-1933 - GAGGAGGACGCUCUGGAGCGGGAC 24 17464

HSV-UL54-1934 - CCAG AAACG CCCACAACC 18 17465

HSV-UL54-1935 - UCCAGAAACGCCCACAACC 19 17466

HSV-UL54-1936 - CUCCAGAAACGCCCACAACC 20 17467

HSV-UL54-1937 - U CU CCAG AAACG CCCACAACC 21 17468

HSV-UL54-1938 - G U CU CCAG AAACGCCCACAACC 22 17469

HSV-UL54-1939 - CG U CU CCAG AAACGCCCACAACC 23 17470

HSV-UL54-1940 - G CG U CU CCAG AAACGCCCACAACC 24 17471

HSV-UL54-1941 - CCGAGGACGCCGGCACCC 18 17472

HSV-UL54-1942 - CCCGAGGACGCCGGCACCC 19 17473

HSV-UL54-710 - CCCCGAGGACGCCGGCACCC 20 7115

HSV-UL54-1943 - GCCCCGAGGACGCCGGCACCC 21 17474

HSV-UL54-1944 - CGCCCCGAGGACGCCGGCACCC 22 17475

HSV-UL54-1945 - CCGCCCCGAGGACGCCGGCACCC 23 17476

HSV-UL54-1946 - CCCGCCCCGAGGACGCCGGCACCC 24 17477

HSV-UL54-1947 - GGCGGUCGGCUUCCCCCC 18 17478

HSV-UL54-1948 - AGGCGGUCGGCUUCCCCCC 19 17479

HSV-UL54-131 - CAGGCGGUCGGCUUCCCCCC 20 6554

HSV-UL54-1949 - CCAGGCGGUCGGCUUCCCCCC 21 17480

HSV-UL54-1950 - ACCAGGCGGUCGGCUUCCCCCC 22 17481

HSV-UL54-1951 - GACCAGGCGGUCGGCUUCCCCCC 23 17482

HSV-UL54-1952 - GGACCAGGCGGUCGGCUUCCCCCC 24 17483

HSV-UL54-1953 - AGGCGGUCGGCUUCCCCC 18 17484

HSV-UL54-1954 - CAGGCGGUCGGCUUCCCCC 19 17485

HSV-UL54-130 - CCAGGCGGUCGGCUUCCCCC 20 6553

HSV-UL54-1955 - ACCAGGCGGUCGGCUUCCCCC 21 17486

HSV-UL54-1956 - GACCAGGCGGUCGGCU UCCCCC 22 17487

HSV-UL54-1957 - GGACCAGGCGGUCGGCUUCCCCC 23 17488

HSV-UL54-1958 - GGGACCAGGCGGUCGGCUUCCCCC 24 17489

HSV-UL54-1959 - GGUCCCCCGGCCCGCCCC 18 17490

HSV-UL54-1960 - AGGUCCCCCGGCCCGCCCC 19 17491

HSV-UL54-709 - AAGGUCCCCCGGCCCGCCCC 20 7114

HSV-UL54-1961 - AAAGGUCCCCCGGCCCGCCCC 21 17492

HSV-UL54-1962 - CAAAGGUCCCCCGGCCCGCCCC 22 17493

HSV-UL54-1963 - CCAAAGGUCCCCCGGCCCGCCCC 23 17494 HSV-UL54-1964 - CCCAAAG GUCCCCCGGCCCGCCCC 24 17495

HSV-UL54-1965 - CAGGCGGUCGGCUUCCCC 18 17496

HSV-UL54-1966 - CCAGGCGGUCGGCUUCCCC 19 17497

HSV-UL54-717 - ACCAGGCGGUCGGCUUCCCC 20 7118

HSV-UL54-1967 - GACCAGGCGGUCGGCUUCCCC 21 17498

HSV-UL54-1968 - GGACCAGGCGGUCGGCU UCCCC 22 17499

HSV-UL54-1969 - GGGACCAGGCGGUCGGCUUCCCC 23 17500

HSV-UL54-1970 - CGGGACCAGGCGGUCGGCU UCCCC 24 17501

HSV-UL54-1971 - AAGGUCCCCCGGCCCGCC 18 17502

HSV-UL54-1972 - AAAGGUCCCCCGGCCCGCC 19 17503

HSV-UL54-708 - CAAAGGUCCCCCGGCCCGCC 20 7113

HSV-UL54-1973 - CCAAAGGUCCCCCGGCCCGCC 21 17504

HSV-UL54-1974 - CCCAAAG GUCCCCCGGCCCGCC 22 17505

HSV-UL54-1975 - UCCCAAAGGUCCCCCGGCCCGCC 23 17506

HSV-UL54-1976 - UUCCCAAAGGUCCCCCGGCCCGCC 24 17507

HSV-UL54-1977 - CCGGCGUGUGGUCGCGCC 18 17508

HSV-UL54-1978 - ACCGGCGUGUGGUCGCGCC 19 17509

HSV-UL54-715 - CACCGGCGUGUGGUCGCGCC 20 7117

HSV-UL54-1979 - CCACCGGCGUGUGGUCGCGCC 21 17510

HSV-UL54-1980 - ACCACCGGCGUGUGGUCGCGCC 22 17511

HSV-UL54-1981 - GACCACCGGCGUGUGGUCGCGCC 23 17512

HSV-UL54-1982 - CGACCACCGGCGUGUGGUCGCGCC 24 17513

HSV-UL54-1983 - GACCAAGGCACCGCAUCC 18 17514

HSV-UL54-1984 - CGACCAAGGCACCGCAUCC 19 17515

HSV-UL54-726 - UCGACCAAGGCACCGCAUCC 20 7120

HSV-UL54-1985 - GUCGACCAAGGCACCGCAUCC 21 17516

HSV-UL54-1986 - CG U CGACCAAG G CACCG CAU CC 22 17517

HSV-UL54-1987 - CCG U CGACCAAG GCACCGCAU CC 23 17518

HSV-UL54-1988 - CCCGUCGACCAAGGCACCGCAUCC 24 17519

HSV-UL54-1989 - CCCGAG UCCGACAGCAGC 18 17520

HSV-UL54-1990 - CCCCGAGUCCGACAGCAGC 19 17521

HSV-UL54-111 - ACCCCGAGUCCGACAGCAGC 20 6534

HSV-UL54-1991 - GACCCCGAGUCCGACAGCAGC 21 17522

HSV-UL54-1992 - CGACCCCGAGUCCGACAGCAGC 22 17523

HSV-UL54-1993 - ACG ACCCCG AG U CCG ACAGCAG C 23 17524

HSV-UL54-1994 - GACGACCCCGAGUCCGACAGCAGC 24 17525

HSV-UL54-1995 - ACCUGUCCGACAGCGAGC 18 17526

HSV-UL54-1996 - GACCUGUCCGACAGCGAGC 19 17527

HSV-UL54-678 - GGACCUG U CCG ACAG CG AG C 20 7093

HSV-UL54-1997 - UGGACCUGUCCGACAGCGAGC 21 17528

HSV-UL54-1998 - UUGGACCUGUCCGACAGCGAGC 22 17529

HSV-UL54-1999 - AU UGGACCUGUCCGACAGCGAGC 23 17530

HSV-UL54-2000 - GAUUGGACCUGUCCGACAGCGAGC 24 17531

HSV-UL54-2001 - CUUCCCCCCGGGAACCGC 18 17532

HSV-UL54-2002 - GCUUCCCCCCGGGAACCGC 19 17533

HSV-UL54-720 - GGCUUCCCCCCGGGAACCGC 20 7119 HSV-UL54-2003 - CGGCUUCCCCCCGGGAACCGC 21 17534

HSV-UL54-2004 - UCGGCUUCCCCCCGGGAACCGC 22 17535

HSV-UL54-2005 - GUCGGCUUCCCCCCGGGAACCGC 23 17536

HSV-UL54-2006 - GGUCGGCUUCCCCCCGGGAACCGC 24 17537

HSV-UL54-2007 - GGGCGGCGAGGUCGCCGC 18 17538

HSV-UL54-2008 - CGGGCGGCGAGGUCGCCGC 19 17539

HSV-UL54-140 - GCGGGCGGCGAGGUCGCCGC 20 6563

HSV-UL54-2009 - GGCGGGCGGCGAGGUCGCCGC 21 17540

HSV-UL54-2010 - AGGCGGGCGGCGAGGUCGCCGC 22 17541

HSV-UL54-2011 - GAGGCGGGCGGCGAGGUCGCCGC 23 17542

HSV-UL54-2012 - CGAGGCGGGCGGCGAGGUCGCCGC 24 17543

HSV-UL54-2013 - CUCGCCCGGCAGCGCGGC 18 17544

HSV-UL54-2014 - CCUCGCCCGGCAGCGCGGC 19 17545

HSV-UL54-126 - GCCUCGCCCGGCAGCGCGGC 20 6549

HSV-UL54-2015 - CGCCUCGCCCGGCAGCGCGGC 21 17546

HSV-UL54-2016 - ACGCCUCGCCCGGCAGCGCGGC 22 17547

HSV-UL54-2017 - GACGCCUCGCCCGGCAGCGCGGC 23 17548

HSV-UL54-2018 - CGACGCCUCGCCCGGCAGCGCGGC 24 17549

HSV-UL54-2019 - CUGUCCGACAGCGAGCUC 18 17550

HSV-UL54-2020 - CCUGUCCGACAGCGAGCUC 19 17551

HSV-UL54-679 - ACCUGUCCGACAGCGAGCUC 20 7094

HSV-UL54-2021 - GACCUGUCCGACAGCGAGCUC 21 17552

HSV-UL54-2022 - GGACCUGUCCGACAGCGAGCUC 22 17553

HSV-UL54-2023 - UGGACCUGUCCGACAGCGAGCUC 23 17554

HSV-UL54-2024 - UUGGACCUGUCCGACAGCGAGCUC 24 17555

HSV-UL54-2025 - AGCUCGAGGAGGACGCUC 18 17556

HSV-UL54-2026 - GAGCUCGAGGAGGACGCUC 19 17557

HSV-UL54-5 - CGAGCUCGAGGAGGACGCUC 20 6428

HSV-UL54-2027 - GCGAGCUCGAGGAGGACGCUC 21 17558

HSV-UL54-2028 - AGCG AGCUCGAGGAGGACGCUC 22 17559

HSV-UL54-2029 - CAGCGAGCUCGAGGAGGACGCUC 23 17560

HSV-UL54-2030 - ACAGCGAGCUCGAGGAGGACGCUC 24 17561

HSV-UL54-2031 - ACCCCGCCGGCGCGUCUC 18 17562

HSV-UL54-2032 - AACCCCGCCGGCGCGUCUC 19 17563

HSV-UL54-2033 - AAACCCCGCCGGCGCGUCUC 20 17564

HSV-UL54-2034 - AAAACCCCGCCGGCGCGUCUC 21 17565

HSV-UL54-2035 - CAAAACCCCGCCGGCGCGUCUC 22 17566

HSV-UL54-2036 - CCAAAACCCCGCCGGCGCGUCUC 23 17567

HSV-UL54-2037 - ACCAAA ACCCCG CCG G CG CG U CU C 24 17568

HSV-UL54-2038 - CCCCGAGUCCGACAGCAG 18 17569

HSV-UL54-2039 - ACCCCG AGUCCGACAGCAG 19 17570

HSV-UL54-110 - GACCCCGAGUCCGACAGCAG 20 6533

HSV-UL54-2040 - CGACCCCGAGUCCGACAGCAG 21 17571

HSV-UL54-2041 - ACGACCCCGAG UCCGACAGCAG 22 17572

HSV-UL54-2042 - GACGACCCCGAGUCCGACAGCAG 23 17573

HSV-UL54-2043 - CGACGACCCCG AGUCCGACAGCAG 24 17574 HSV-UL54-2044 - GCUCUGGAGCGGGACGAG 18 17575

HSV-UL54-2045 - CGCUCUGGAGCGGGACGAG 19 17576

HSV-UL54-692 - ACGCUCUGGAGCGGGACGAG 20 7104

HSV-UL54-2046 - GACGCUCUGGAGCGGGACGAG 21 17577

HSV-UL54-2047 - GGACGCUCUGGAGCGGGACGAG 22 17578

HSV-UL54-2048 - AGGACGCUCUGGAGCGGGACGAG 23 17579

HSV-UL54-2049 - GAGGACGCUCUGGAGCGGGACGAG 24 17580

HSV-UL54-2050 - AGGCACCGCAUCCCCGAG 18 17581

HSV-UL54-2051 - AAGGCACCGCAUCCCCGAG 19 17582

HSV-UL54-727 - CAAGGCACCGCAUCCCCGAG 20 7121

HSV-UL54-2052 - CCAAGGCACCGCAUCCCCGAG 21 17583

HSV-UL54-2053 - ACCAAGGCACCGCAUCCCCGAG 22 17584

HSV-UL54-2054 - GACCAAG G CACCG CAU CCCCG AG 23 17585

HSV-UL54-2055 - CGACCAAGGCACCGCAUCCCCGAG 24 17586

HSV-UL54-2056 - UCCGACAGCGAGCUCGAG 18 17587

HSV-UL54-2057 - GUCCGACAGCGAGCUCGAG 19 17588

HSV-UL54-687 - UGUCCGACAGCGAGCUCGAG 20 7100

HSV-UL54-2058 - CUGUCCGACAGCGAGCUCGAG 21 17589

HSV-UL54-2059 - CCUGUCCGACAGCGAGCUCGAG 22 17590

HSV-UL54-2060 - ACCUGUCCGACAGCGAGCUCGAG 23 17591

HSV-UL54-2061 - GACCUGUCCGACAGCGAGCUCGAG 24 17592

HSV-UL54-2062 - UGGAAGACCCCUGCGGAG 18 17593

HSV-UL54-2063 - AUGGAAGACCCCUGCGGAG 19 17594

HSV-UL54-682 - CAUGGAAGACCCCUGCGGAG 20 7096

HSV-UL54-2064 - ACAUGGAAGACCCCUGCGGAG 21 17595

HSV-UL54-2065 - GACAUGGAAGACCCCUGCGGAG 22 17596

HSV-UL54-2066 - GGACAUGGAAGACCCCUGCGGAG 23 17597

HSV-UL54-2067 - AGGACAUGGAAGACCCCUGCGGAG 24 17598

HSV-UL54-2068 - GAGGAGGACGCUCUGGAG 18 17599

HSV-UL54-2069 - CGAGGAGGACGCUCUGGAG 19 17600

HSV-UL54-105 - UCGAGGAGGACGCUCUGGAG 20 6528

HSV-UL54-2070 - CUCGAGGAGGACGCUCUGGAG 21 17601

HSV-UL54-2071 - GCUCGAGGAGGACGCUCUGGAG 22 17602

HSV-UL54-2072 - AGCUCGAGGAGGACGCUCUGGAG 23 17603

HSV-UL54-2073 - GAGCUCGAGGAGGACGCUCUGGAG 24 17604

HSV-UL54-2074 - CCCCCCGGGAACCGCACG 18 17605

HSV-UL54-2075 - UCCCCCCGGGAACCGCACG 19 17606

HSV-UL54-17 - UUCCCCCCGGGAACCGCACG 20 6440

HSV-UL54-2076 - CUUCCCCCCGGGAACCGCACG 21 17607

HSV-UL54-2077 - GCUUCCCCCCGGGAACCGCACG 22 17608

HSV-UL54-2078 - GGCUUCCCCCCGGGAACCGCACG 23 17609

HSV-UL54-2079 - CGGCUUCCCCCCGGGAACCGCACG 24 17610

HSV-UL54-2080 - AAGACCCCUGCGGAGACG 18 17611

HSV-UL54-2081 - GAAGACCCCUGCGGAGACG 19 17612

HSV-UL54-704 - GGAAGACCCCUGCGGAGACG 20 7111

HSV-UL54-2082 - UGGAAGACCCCUGCGGAGACG 21 17613 HSV-UL54-2083 - AUGGAAGACCCCUGCGGAGACG 22 17614

HSV-UL54-2084 - CAUGGAAGACCCCUGCGGAGACG 23 17615

HSV-UL54-2085 - ACAUGGAAGACCCCUGCGGAGACG 24 17616

HSV-UL54-2086 - ACGCUCUGGAGCGGGACG 18 17617

HSV-UL54-2087 - GACGCUCUGGAGCGGGACG 19 17618

HSV-UL54-107 - GGACGCUCUGGAGCGGGACG 20 6530

HSV-UL54-2088 - AGGACGCUCUGGAGCGGGACG 21 17619

HSV-UL54-2089 - GAGG ACGCUCUGGAGCGGGACG 22 17620

HSV-UL54-2090 - GG AGGACGCUCUGGAGCGGGACG 23 17621

HSV-UL54-2091 - AGGAGGACGCUCUGGAGCGGGACG 24 17622

HSV-UL54-2092 - CGGGCGGCGAGGUCGCCG 18 17623

HSV-UL54-2093 - GCGGGCGGCGAGGUCGCCG 19 17624

HSV-UL54-729 - GGCGGGCGGCGAGGUCGCCG 20 7123

HSV-UL54-2094 - AGGCGGGCGGCGAGGUCGCCG 21 17625

HSV-UL54-2095 - GAGGCGGGCGGCGAGGUCGCCG 22 17626

HSV-UL54-2096 - CG AGGCGGGCGGCGAGGUCGCCG 23 17627

HSV-UL54-2097 - CCGAGGCGGGCGGCGAGGUCGCCG 24 17628

HSV-UL54-2098 - GCCUCGCCCGGCAGCGCG 18 17629

HSV-UL54-2099 - CGCCUCGCCCGGCAGCGCG 19 17630

HSV-UL54-711 - ACGCCUCGCCCGGCAGCGCG 20 7116

HSV-UL54-2100 - GACGCCUCGCCCGGCAGCGCG 21 17631

HSV-UL54-2101 - CGACGCCUCGCCCGGCAGCGCG 22 17632

HSV-UL54-2102 - UCGACGCCUCGCCCGGCAGCGCG 23 17633

HSV-UL54-2103 - CUCGACGCCUCGCCCGGCAGCGCG 24 17634

HSV-UL54-2104 - UCGCCCGGCAGCGCGGCG 18 17635

HSV-UL54-2105 - CUCGCCCGGCAGCGCGGCG 19 17636

HSV-UL54-127 - CCUCGCCCGGCAGCGCGGCG 20 6550

HSV-UL54-2106 - GCCUCGCCCGGCAGCGCGGCG 21 17637

HSV-UL54-2107 - CGCCUCGCCCGGCAGCGCGGCG 22 17638

HSV-UL54-2108 - ACGCCUCGCCCGGCAGCGCGGCG 23 17639

HSV-UL54-2109 - GACGCCUCGCCCGGCAGCGCGGCG 24 17640

HSV-UL54-2110 - GCAUCCCCGAGGCGGGCG 18 17641

HSV-UL54-2111 - CGCAUCCCCGAGGCGGGCG 19 17642

HSV-UL54-728 - CCGCAUCCCCGAGGCGGGCG 20 7122

HSV-UL54-2112 - ACCGCAUCCCCGAGGCGGGCG 21 17643

HSV-UL54-2113 - CACCGCAUCCCCGAGGCGGGCG 22 17644

HSV-UL54-2114 - GCACCGCAUCCCCGAGGCGGGCG 23 17645

HSV-UL54-2115 - GGCACCGCAUCCCCGAGGCGGGCG 24 17646

HSV-UL54-2116 - AGCGGGGAGUGUUCCUCG 18 17647

HSV-UL54-2117 - CAGCGGGGAGUGUUCCUCG 19 17648

HSV-UL54-697 - GCAGCGGGGAGUGUUCCUCG 20 7107

HSV-UL54-2118 - AGCAGCGGGGAGUGUUCCUCG 21 17649

HSV-UL54-2119 - CAGCAGCGGGGAGUGUUCCUCG 22 17650

HSV-UL54-2120 - ACAGCAGCGGGGAGUGUUCCUCG 23 17651

HSV-UL54-2121 - GACAGCAGCGGGGAGUGUUCCUCG 24 17652

HSV-UL54-2122 - UGUCCGACAGCGAGCUCG 18 17653 HSV-UL54-2123 - CUGUCCGACAGCGAGCUCG 19 17654

HSV-UL54-3 - CCUGUCCGACAGCGAGCUCG 20 6426

HSV-UL54-2124 - ACCUGUCCGACAGCGAGCUCG 21 17655

HSV-UL54-2125 - GACCUGUCCGACAGCGAGCUCG 22 17656

HSV-UL54-2126 - GG ACCUGUCCGACAGCGAGCUCG 23 17657

HSV-UL54-2127 - UGGACCUGUCCGACAGCGAGCUCG 24 17658

HSV-UL54-2128 - CCCCCGGGAACCGCACGG 18 17659

HSV-UL54-2129 - CCCCCCGGGAACCGCACGG 19 17660

HSV-UL54-18 - UCCCCCCGGGAACCGCACGG 20 6441

HSV-UL54-2130 - UUCCCCCCGGGAACCGCACGG 21 17661

HSV-UL54-2131 - CUUCCCCCCGGGAACCGCACGG 22 17662

HSV-UL54-2132 - GCUUCCCCCCGGGAACCGCACGG 23 17663

HSV-UL54-2133 - GGCUUCCCCCCGGGAACCGCACGG 24 17664

HSV-UL54-2134 - AGACCCCUGCGGAGACGG 18 17665

HSV-UL54-2135 - AAGACCCCUGCGGAGACGG 19 17666

HSV-UL54-117 - GAAGACCCCUGCGGAGACGG 20 6540

HSV-UL54-2136 - GGAAGACCCCUGCGGAGACGG 21 17667

HSV-UL54-2137 - UGGAAGACCCCUGCGGAGACGG 22 17668

HSV-UL54-2138 - AUGG AAGACCCCUGCGGAGACGG 23 17669

HSV-UL54-2139 - CAUGGAAGACCCCUGCGGAGACGG 24 17670

HSV-UL54-2140 - CCUCGCCCGGCAGCGCGG 18 17671

HSV-UL54-2141 - GCCUCGCCCGGCAGCGCGG 19 17672

HSV-UL54-125 - CGCCUCGCCCGGCAGCGCGG 20 6548

HSV-UL54-2142 - ACGCCUCGCCCGGCAGCGCGG 21 17673

HSV-UL54-2143 - GACGCCUCGCCCGGCAGCGCGG 22 17674

HSV-UL54-2144 - CGACGCCUCGCCCGGCAGCGCGG 23 17675

HSV-UL54-2145 - UCGACGCCUCGCCCGGCAGCGCGG 24 17676

HSV-UL54-2146 - GGGAGUGUUCCUCGUCGG 18 17677

HSV-UL54-2147 - GGGGAGUGUUCCUCGUCGG 19 17678

HSV-UL54-698 - CGGGGAGUGUUCCUCGUCGG 20 7108

HSV-UL54-2148 - GCGGGGAGUGUUCCUCGUCGG 21 17679

HSV-UL54-2149 - AGCGGGGAGUGUUCCUCGUCGG 22 17680

HSV-UL54-2150 - CAGCGGGGAGUGUUCCUCGUCGG 23 17681

HSV-UL54-2151 - GCAGCGGGGAGUGUUCCUCGUCGG 24 17682

HSV-UL54-2152 - CCCUGCGGAGACGGAGGG 18 17683

HSV-UL54-2153 - CCCCUGCGGAGACGGAGGG 19 17684

HSV-UL54-706 - ACCCCUGCGGAGACGGAGGG 20 7112

HSV-UL54-2154 - GACCCCUGCGGAGACGGAGGG 21 17685

HSV-UL54-2155 - AG ACCCCUGCGGAGACGGAGGG 22 17686

HSV-UL54-2156 - AAGACCCCUGCGGAGACGGAGGG 23 17687

HSV-UL54-2157 - GAAGACCCCUGCGGAGACGGAGGG 24 17688

HSV-UL54-2158 - CCCCGGGAACCGCACGGG 18 17689

HSV-UL54-2159 - CCCCCGGGAACCGCACGGG 19 17690

HSV-UL54-19 - CCCCCCGGGAACCGCACGGG 20 6442

HSV-UL54-2160 - UCCCCCCGGGAACCGCACGGG 21 17691

HSV-UL54-2161 - UUCCCCCCGGGAACCGCACGGG 22 17692 HSV-UL54-2162 - CUUCCCCCCGGGAACCGCACGGG 23 17693

HSV-UL54-2163 - GCUUCCCCCCGGGAACCGCACGGG 24 17694

HSV-UL54-2164 - AGGACGCUCUGGAGCGGG 18 17695

HSV-UL54-2165 - GAGGACGCUCUGGAGCGGG 19 17696

HSV-UL54-691 - GGAGGACGCUCUGGAGCGGG 20 7103

HSV-UL54-2166 - AGGAGGACGCUCUGGAGCGGG 21 17697

HSV-UL54-2167 - GAGG AGGACGCUCUGGAGCGGG 22 17698

HSV-UL54-2168 - CGAGGAGGACGCUCUGGAGCGGG 23 17699

HSV-UL54-2169 - UCGAGGAGGACGCUCUGGAGCGGG 24 17700

HSV-UL54-2170 - CCUGCGGAGACGGAGGGG 18 17701

HSV-UL54-2171 - CCCUGCGGAGACGGAGGGG 19 17702

HSV-UL54-119 - CCCCUGCGGAGACGGAGGGG 20 6542

HSV-UL54-2172 - ACCCCUGCGGAGACGGAGGGG 21 17703

HSV-UL54-2173 - GACCCCUGCGGAGACGGAGGGG 22 17704

HSV-UL54-2174 - AGACCCCUGCGGAGACGGAGGGG 23 17705

HSV-UL54-2175 - AAGACCCCUGCGGAGACGGAGGGG 24 17706

HSV-UL54-2176 - GCGAGGUCGCCGCCGGGG 18 17707

HSV-UL54-2177 - GGCGAGGUCGCCGCCGGGG 19 17708

HSV-UL54-731 - CGGCGAGGUCGCCGCCGGGG 20 7124

HSV-UL54-2178 - GCGGCGAGGUCGCCGCCGGGG 21 17709

HSV-UL54-2179 - GGCGGCGAGGUCGCCGCCGGGG 22 17710

HSV-UL54-2180 - GGGCGGCGAGGUCGCCGCCGGGG 23 17711

HSV-UL54-2181 - CGGGCGGCGAGGUCGCCGCCGGGG 24 17712

HSV-UL54-2182 - GGACAUGGAAGACCCCUG 18 17713

HSV-UL54-2183 - AGGACAUGGAAGACCCCUG 19 17714

HSV-UL54-115 - GAGGACAUGGAAGACCCCUG 20 6538

HSV-UL54-2184 - CGAGGACAUGGAAGACCCCUG 21 17715

HSV-UL54-2185 - ACGAGGACAUGGAAGACCCCUG 22 17716

HSV-UL54-2186 - GACGAGGACAUGGAAGACCCCUG 23 17717

HSV-UL54-2187 - GGACGAGGACAUGGAAGACCCCUG 24 17718

HSV-UL54-2188 - AGGACAUGGAAGACCCCU 18 17719

HSV-UL54-2189 - GAGGACAUGGAAGACCCCU 19 17720

HSV-UL54-681 - CG AGGACAUGGAAGACCCCU 20 7095

HSV-UL54-2190 - ACGAGGACAUGGAAGACCCCU 21 17721

HSV-UL54-2191 - GACGAGGACAUGGAAGACCCCU 22 17722

HSV-UL54-2192 - GGACG AGGACAUGGAAGACCCCU 23 17723

HSV-UL54-2193 - CGGACGAGGACAUGGAAGACCCCU 24 17724

HSV-UL54-2194 - CGGCGUGUGGUCGCGCCU 18 17725

HSV-UL54-2195 - CCGGCGUGUGGUCGCGCCU 19 17726

HSV-UL54-128 - ACCGGCGUGUGGUCGCGCCU 20 6551

HSV-UL54-2196 - CACCGGCGUGUGGUCGCGCCU 21 17727

HSV-UL54-2197 - CCACCGGCGUGUGGUCGCGCCU 22 17728

HSV-UL54-2198 - ACCACCGGCGUGUGGUCGCGCCU 23 17729

HSV-UL54-2199 - GACCACCGGCGUGUGGUCGCGCCU 24 17730

HSV-UL54-2200 - GAGCUCGAGGAGGACGCU 18 17731

HSV-UL54-2201 - CGAGCUCGAGGAGGACGCU 19 17732 HSV-UL54-688 - GCGAGCUCGAGGAGGACGCU 20 7101

HSV-UL54-2202 - AGCGAGCUCGAGGAGGACGCU 21 17733

HSV-UL54-2203 - CAGCGAGCUCGAGGAGGACGCU 22 17734

HSV-UL54-2204 - ACAGCGAGCUCGAGGAGGACGCU 23 17735

HSV-UL54-2205 - GACAGCGAGCUCGAGGAGGACGCU 24 17736

Table 15E provides exemplary targeting domains for knocking out the UL54 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene), and start with a 5'G. The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 15E

HSV-UL54-2227 + GACGCGGUUGGCGAGGCGGGC 21 17758

HSV-UL54-1412 + GCCGUCCGUCCGCGCCGACG 20 16943

HSV-UL54-2228 + GGCCGUCCGUCCGCGCCGACG 21 17759

HSV-UL54-2229 + GGGCCGUCCGUCCGCGCCGACG 22 17760

HSV-UL54-2230 + GGGGCCGUCCGUCCGCGCCGACG 23 17761

HSV-UL54-2231 + GGUU UCCCAGGAAACCCG 18 17762

HSV-UL54-2232 + GGGUU UCCCAGGAAACCCG 19 17763

HSV-UL54-2233 + G ACCAGGG U U UCCCAGGAAACCCG 24 17764

HSV-UL54-2234 + GCAGAACCGCCCGAACGG 18 17765

HSV-UL54-2235 + GAUCGCAGAACCGCCCGAACGG 22 17766

HSV-UL54-2236 + GGAUCGCAGAACCGCCCGAACGG 23 17767

HSV-UL54-1480 + GUUUCUGGAGACGCGCCGG 19 17011

HSV-UL54-1481 + GCGUUUCUGGAGACGCGCCGG 21 17012

HSV-UL54-1482 + GGCGUUUCUGGAGACGCGCCGG 22 17013

HSV-UL54-1483 + GGGCGUU UCUGGAGACGCGCCGG 23 17014

HSV-UL54-2237 + GCGGCCGUCCGCGUGCGG 18 17768

HSV-UL54-1436 + GCGCGGCCGUCCGCGUGCGG 20 16967

HSV-UL54-2238 + GGCGCGGCCGUCCGCGUGCGG 21 17769

HSV-UL54-2239 + GGGCGCGGCCGUCCGCGUGCGG 22 17770

HSV-UL54-2240 + GGGGCGCGGCCGUCCGCGUGCGG 23 17771

HSV-UL54-2241 + GCGGAGCGGCAGAUUGUG 18 17772

HSV-UL54-2242 + GGCGGAGCGGCAGAUUGUG 19 17773

HSV-UL54-2243 + GGGCGGAGCGGCAGAUUGUG 20 17774

HSV-UL54-2244 + GGGGCGGAGCGGCAGAUUGUG 21 17775

HSV-UL54-2245 + GGGGGCGGAGCGGCAGAUUGUG 22 17776

HSV-UL54-2246 + GACCGCCGCGCGCUCGGAU 19 17777

HSV-UL54-2247 + GCUCGACCGCCGCGCGCUCGGAU 23 17778

HSV-UL54-2248 + GCGGCCCGCGGGUUGGCU 18 17779

HSV-UL54-2249 + GACGCGGCCCGCGGGUUGGCU 21 17780

HSV-UL54-2250 + GUCGACGCGGCCCGCGGGUUGGCU 24 17781

HSV-UL54-1490 - G U CUCCAG AAACG CCCACAACCAA 24 17021

HSV-UL54-2251 - GAAAUACUGGACACGCACCGCC 22 17782

HSV-UL54-2252 - GCAACUCCCUAU UUUAGGC 19 17783

HSV-UL54-2253 - G U U U G ACGCCG AG ACCCG 18 17784

HSV-UL54-2254 - GGUUUGACGCCGAGACCCG 19 17785

HSV-UL54-939 - GGGUUUGACGCCGAG ACCCG 20 7282

HSV-UL54-2255 - GGGGUUUGACGCCGAGACCCG 21 17786

HSV-UL54-2256 - GGGGGUUUGACGCCGAG ACCCG 22 17787

HSV-UL54-2257 - GGGGGGUUUGACGCCGAGACCCG 23 17788

HSV-UL54-2258 - GGGGGGGUUUGACGCCGAGACCCG 24 17789

HSV-UL54-2259 - GUGCUGGCCACCCAAGCGG 19 17790

HSV-UL54-306 - GUCAUGCAAGACCCCUUUGG 20 6729

HSV-UL54-2260 - GGUCAUGCAAGACCCCUUUGG 21 17791

HSV-UL54-2261 - GAUCGACUACACGACCGU 18 17792

HSV-UL54-350 - GAGAUCGACUACACGACCGU 20 6773

HSV-UL54-2262 - GGAGAUCGACUACACGACCGU 21 17793 HSV-UL54-2263 - GUCGGAGAUCGACUACACGACCGU 24 17794

HSV-UL54-2264 - GCGACGCCUGUCGGACAUU 19 17795

HSV-UL54-960 - GGCGACGCCUGUCGGACAUU 20 7297

HSV-UL54-2265 - GCGGCGACGCCUGUCGGACAUU 22 17796

Table 15F provides exemplary targeting domains for knocking out the UL54 gene selected according to the six tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRT. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 15F

HSV-UL54-2289 + UGGCCUUGGCUGUCGACGCGGCCC 24 17820

HSV-UL54-2290 + UCCGCGCCGCGCCUCGCC 18 17821

HSV-UL54-2291 + CUCCGCGCCGCGCCUCGCC 19 17822

HSV-UL54-2292 + CCUCCGCGCCGCGCCUCGCC 20 17823

HSV-UL54-2293 + CCCUCCGCGCCGCGCCUCGCC 21 17824

HSV-UL54-2294 + CCCCUCCGCGCCGCGCCUCGCC 22 17825

HSV-UL54-2295 + UCCCCUCCGCGCCGCGCCUCGCC 23 17826

HSV-UL54-2296 + CUCCCCUCCGCGCCGCGCCUCGCC 24 17827

HSV-UL54-2297 + CGGCGUCAAACCCCCCCGC 19 17828

HSV-UL54-2298 + UCGGCGUCAAACCCCCCCGC 20 17829

HSV-UL54-2299 + CUCGGCGUCAAACCCCCCCGC 21 17830

HSV-UL54-2300 + UCUCGGCGUCAAACCCCCCCGC 22 17831

HSV-UL54-2301 + CGCUCGACCGCCGCGCGC 18 17832

HSV-UL54-2302 + UGCGCUCGACCGCCGCGCGC 20 17833

HSV-UL54-2303 + AUGCGCUCGACCGCCGCGCGC 21 17834

HSV-UL54-2304 + UGAUGCGCUCGACCGCCGCGCGC 23 17835

HSV-UL54-2305 + CUGAUGCGCUCGACCGCCGCGCGC 24 17836

HSV-UL54-2306 + CGCGGUUGGCGAGGCGGGC 19 17837

HSV-UL54-2307 + ACGCGGUUGGCGAGGCGGGC 20 17838

HSV-UL54-2308 + CGACGCGGUUGGCGAGGCGGGC 22 17839

HSV-UL54-2309 + UCGACGCGGUUGGCGAGGCGGGC 23 17840

HSV-UL54-2310 + CUCGACGCGGUUGGCGAGGCGGGC 24 17841

HSV-UL54-2311 + CGUCCGUCCGCGCCGACG 18 17842

HSV-UL54-2312 + CCGUCCGUCCGCGCCGACG 19 17843

HSV-UL54-2313 + CGGGGCCGUCCGUCCGCGCCGACG 24 17844

HSV-UL54-2314 + AGGGU U UCCCAGGAAACCCG 20 17845

HSV-UL54-2315 + CAGGGUUUCCCAGGAAACCCG 21 17846

HSV-UL54-2316 + CCAGGG U U U CCCAG G AAACCCG 22 17847

HSV-UL54-2317 + ACCAGGGUUU CCCAG G AAACCCG 23 17848

HSV-UL54-2318 + CG CAG AACCGCCCG AACG G 19 17849

HSV-UL54-1432 + UCG CAG AACCG CCCG AACG G 20 16963

HSV-UL54-2319 + AUCGCAG AACCGCCCG AACGG 21 17850

HSV-UL54-2320 + UGGAUCGCAGAACCGCCCGAACGG 24 17851

HSV-UL54-1516 + UUUCUGGAGACGCGCCGG 18 17047

HSV-UL54-1220 + CGUUUCUGGAGACGCGCCGG 20 16751

HSV-UL54-1517 + UGGGCGUUUCUGGAGACGCGCCGG 24 17048

HSV-UL54-2321 + CGCGGCCGUCCGCGUGCGG 19 17852

HSV-UL54-2322 + CGGGGCGCGGCCGUCCGCGUGCGG 24 17853

HSV-UL54-2323 + UGGGGGCGGAGCGGCAGAUUGUG 23 17854

HSV-UL54-2324 + CUGGGGGCGGAGCGGCAGAUUGUG 24

17855

HSV-UL54-2325 + ACCGCCGCGCGCUCGGAU 18 17856

HSV-UL54-2326 + CGACCGCCGCGCGCUCGGAU 20 17857

HSV-UL54-2327 + UCGACCGCCGCGCGCUCGGAU 21 17858

HSV-UL54-2328 + CUCGACCGCCGCGCGCUCGGAU 22 17859

HSV-UL54-2329 + CGCUCGACCGCCGCGCGCUCGGAU 24 17860 HSV-UL54-2330 + CGCGGCCCGCGGGUUGGCU 19 17861

HSV-UL54-2331 + ACGCGGCCCGCGGGUUGGCU 20 17862

HSV-UL54-2332 + CGACGCGGCCCGCGGGUUGGCU 22 17863

HSV-UL54-2333 + UCGACGCGGCCCGCGGGUUGGCU 23 17864

HSV-UL54-1561 - AGAAACGCCCACAACCAA 18 17092

HSV-UL54-1562 - CAGAAACGCCCACAACCAA 19 17093

HSV-UL54-1234 - CCAGAAACGCCCACAACCAA 20 16765

HSV-UL54-1539 - UCCAGAAACGCCCACAACCAA 21 17070

HSV-UL54-1540 - CU CCAG AAACG CCCACAACCAA 22 17071

HSV-UL54-1541 - U CU CCAGAAACGCCCACAACCAA 23 17072

HSV-UL54-2334 - UACUGGACACGCACCGCC 18 17865

HSV-UL54-2335 - AUACUGGACACGCACCGCC 19 17866

HSV-UL54-365 - AAUACUGGACACGCACCGCC 20 6788

HSV-UL54-2336 - AAAUACUGGACACGCACCGCC 21 17867

HSV-UL54-2337 - UGAAAUACUGGACACGCACCGCC 23 17868

HSV-UL54-2338 - UUGAAAUACUGGACACGCACCGCC 24 17869

HSV-UL54-2339 - AUCGAGGCCCUGGCGUCC 18 17870

HSV-UL54-2340 - CAUCGAGGCCCUGGCGUCC 19 17871

HSV-UL54-948 - UCAUCGAGGCCCUGGCGUCC 20 7288

HSV-UL54-2341 - CUCAUCGAGGCCCUGGCGUCC 21 17872

HSV-UL54-2342 - UCUCAUCGAGGCCCUGGCGUCC 22 17873

HSV-UL54-2343 - AUCUCAUCGAGGCCCUGGCGUCC 23 17874

HSV-UL54-2344 - AAUCUCAUCGAGGCCCUGGCGUCC 24 17875

HSV-UL54-2345 - CAACUCCCUAUUUUAGGC 18 17876

HSV-UL54-2346 - UGCAACUCCCUAUUUUAGGC 20 17877

HSV-UL54-2347 - CUGCAACUCCCUAUUUUAGGC 21 17878

HSV-UL54-2348 - ACUGCAACUCCCUAUUUUAGGC 22 17879

HSV-UL54-2349 - UACUGCAACUCCCUAUUUUAGGC 23 17880

HSV-UL54-2350 - CUACUGCAACUCCCUAUUUUAGGC 24 17881

HSV-UL54-2351 - UGCUGGCCACCCAAGCGG 18 17882

HSV-UL54-314 - CGUGCUGGCCACCCAAGCGG 20 6737

HSV-UL54-2352 - CCGUGCUGGCCACCCAAGCGG 21 17883

HSV-UL54-2353 - CCCGUGCUGGCCACCCAAGCGG 22 17884

HSV-UL54-2354 - UCCCGUGCUGGCCACCCAAGCGG 23 17885

HSV-UL54-2355 - CUCCCGUGCUGGCCACCCAAGCGG 24 17886

HSV-UL54-2356 - CAUGCAAGACCCCU UUGG 18 17887

HSV-UL54-2357 - UCAUGCAAGACCCCUUUGG 19 17888

HSV-UL54-2358 - UGGUCAUGCAAGACCCCUUUGG 22 17889

HSV-UL54-2359 - CUGGUCAUGCAAGACCCCUUUGG 23 17890

HSV-UL54-2360 - CCU GG U CAU G CAAG ACCCCU U UG G 24 17891

HSV-UL54-2361 - AUCCCGGGGGCCUGCAUG 18 17892

HSV-UL54-2362 - CAUCCCGGGGGCCUGCAUG 19 17893

HSV-UL54-973 - ACAUCCCGGGGGCCUGCAUG 20 7304

HSV-UL54-2363 - UACAUCCCGGGGGCCUGCAUG 21 17894

HSV-UL54-2364 - UUACAUCCCGGGGGCCUGCAUG 22 17895

HSV-UL54-2365 - UUUACAUCCCGGGGGCCUGCAUG 23 17896 HSV-UL54-2366 - UUUUACAUCCCGGGGGCCUGCAUG 24 17897

HSV-UL54-2367 - AGAUCGACUACACGACCGU 19 17898

HSV-UL54-2368 - CGGAGAUCGACUACACGACCGU 22 17899

HSV-UL54-2369 - UCGG AGAUCGACUACACGACCGU 23 17900

HSV-UL54-2370 - CGACGCCUGUCGGACAUU 18 17901

HSV-UL54-2371 - CGGCGACGCCUGUCGGACAUU 21 17902

HSV-UL54-2372 - CGCGGCGACGCCUGUCGGACAUU 23 17903

HSV-UL54-2373 - UCGCGGCGACGCCUGUCGGACAUU 24 17904

Table 15G provides exemplary targeting domains for knocking out the UL54 gene selected according to the seven tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). The PAM is NNGRRV. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a S. aureus Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single-stranded break (Cas9 nickase).

Table 15G

HSV-UL54-2392 + GGGCCUUCAGGUAGCACUGCAGAA 24 17923

HSV-UL54-2393 + UUUGCCGUGCACAUAUAA 18 17924

HSV-UL54-2394 + AUUUGCCGUGCACAUAUAA 19 17925

HSV-UL54-1361 + UAU UUGCCGUGCACAUAUAA 20 16892

HSV-UL54-2395 + GUAUUUGCCGUGCACAUAUAA 21 17926

HSV-UL54-2396 + AGUAUUUGCCGUGCACAUAUAA 22 17927

HSV-UL54-2397 + AAG UAUUUGCCGUGCACAUAUAA 23 17928

HSV-UL54-2398 + GAAGUAUUUGCCGUGCACAUAUAA 24 17929

HSV-UL54-2399 + GCCAGGAUGACCAACACA 18 17930

HSV-UL54-2400 + GGCCAGGAUGACCAACACA 19 17931

HSV-UL54-2401 + GGGCCAGGAUGACCAACACA 20 17932

HSV-UL54-2402 + CG G G CCAG G AU G ACCAACACA 21 17933

HSV-UL54-2403 + GCGGGCCAGGAUGACCAACACA 22 17934

HSV-UL54-2404 + GGCGGGCCAGGAUGACCAACACA 23 17935

HSV-UL54-2405 + AGGCGGGCCAGGAUGACCAACACA 24 17936

HSV-UL54-2406 + GGAAACGGCAUCCCGCCA 18 17937

HSV-UL54-2407 + GGGAAACGGCAUCCCGCCA 19 17938

HSV-UL54-2408 + CG G G AAACG G CAU CCCG CCA 20 17939

HSV-UL54-2409 + GCGGGAAACGGCAUCCCGCCA 21 17940

HSV-UL54-2410 + GGCGGGAAACGGCAUCCCGCCA 22 17941

HSV-UL54-2411 + CGGCGGGAAACGGCAUCCCGCCA 23 17942

HSV-UL54-2412 + GCGGCGGGAAACGGCAUCCCGCCA 24 17943

HSV-UL54-2413 + CCGCUUGGGUGGCCAGCA 18 17944

HSV-UL54-2414 + CCCGCUUGGGUGGCCAGCA 19 17945

HSV-UL54-419 + CCCCGCUUGGGUGGCCAGCA 20 6842

HSV-UL54-2415 + CCCCCGCUUGGGUGGCCAGCA 21 17946

HSV-UL54-2416 + CCCCCCGCUUGGGUGGCCAGCA 22 17947

HSV-UL54-2417 + CCCCCCCGCUUGGGUGGCCAGCA 23 17948

HSV-UL54-2418 + ACCCCCCCGCUUGGGUGGCCAGCA 24 17949

HSV-UL54-2419 + GGGUCGAUGGUGUCGGCA 18 17950

HSV-UL54-2420 + GGGGUCGAUGGUGUCGGCA 19 17951

HSV-UL54-2421 + CGGGGUCGAUGGUGUCGGCA 20 17952

HSV-UL54-2422 + GCGGGGUCGAUGGUGUCGGCA 21 17953

HSV-UL54-2423 + GGCGGGGUCGAUGGUGUCGGCA 22 17954

HSV-UL54-2424 + CGGCGGGGUCGAUGGUGUCGGCA 23 17955

HSV-UL54-2425 + ACGGCGGGGUCGAUGGUGUCGGCA 24 17956

HSV-UL54-2426 + UCAGGGACAGCGCCAUCA 18 17957

HSV-UL54-2427 + GUCAGGGACAGCGCCAUCA 19 17958

HSV-UL54-2428 + GG UCAGGGACAGCGCCAUCA 20 17959

HSV-UL54-2429 + GGGUCAGGGACAGCGCCAUCA 21 17960

HSV-UL54-2430 + GGGGUCAGGGACAGCGCCAUCA 22 17961

HSV-UL54-2431 + GGGGG UCAGGGACAGCGCCAUCA 23 17962

HSV-UL54-2432 + GGGGGGUCAGGGACAGCGCCAUCA 24 17963

HSV-UL54-2433 + UGCGUGUCCAGUAUUUCA 18 17964

HSV-UL54-2434 + GUGCGUGUCCAGUAUUUCA 19 17965

HSV-UL54-2435 + GGUGCGUGUCCAGUAUUUCA 20 17966 HSV-UL54-2436 + CGGUGCGUGUCCAGUAUUUCA 21 17967

HSV-UL54-2437 + GCGGUGCGUGUCCAGUAUUUCA 22 17968

HSV-UL54-2438 + GGCGGUGCGUGUCCAGUAUUUCA 23 17969

HSV-UL54-2439 + UGGCGGUGCGUGUCCAGUAUUUCA 24 17970

HSV-UL54-2440 + UUCAGGUAGCACUGCAGA 18 17971

HSV-UL54-2441 + CUUCAGGUAGCACUGCAGA 19 17972

HSV-UL54-2442 + CCUUCAGGUAGCACUGCAGA 20 17973

HSV-UL54-2443 + GCCUUCAGGUAGCACUGCAGA 21 17974

HSV-UL54-2444 + GGCCUUCAGGUAGCACUGCAGA 22 17975

HSV-UL54-2445 + GGGCCUUCAGGUAGCACUGCAGA 23 17976

HSV-UL54-2446 + CGGGCCUUCAGGUAGCACUGCAGA 24 17977

HSV-UL54-2447 + GUCAGCUCGCACACGCGA 18 17978

HSV-UL54-2448 + CGUCAGCUCGCACACGCGA 19 17979

HSV-UL54-2449 + CCGUCAGCUCGCACACGCGA 20 17980

HSV-UL54-2450 + GCCGUCAGCUCGCACACGCGA 21 17981

HSV-UL54-2451 + GGCCGUCAGCUCGCACACGCGA 22 17982

HSV-UL54-2452 + UGGCCGUCAGCUCGCACACGCGA 23 17983

HSV-UL54-2453 + CUGGCCGUCAGCUCGCACACGCGA 24 17984

HSV-UL54-2454 + GACAGCGCCAUCAGCGGA 18 17985

HSV-UL54-2455 + GGACAGCGCCAUCAGCGGA 19 17986

HSV-UL54-1371 + GGGACAGCGCCAUCAGCGGA 20 16902

HSV-UL54-2456 + AGGGACAGCGCCAUCAGCGGA 21 17987

HSV-UL54-2457 + CAGGGACAGCGCCAUCAGCGGA 22 17988

HSV-UL54-2458 + UCAGGGACAGCGCCAUCAGCGGA 23 17989

HSV-UL54-2459 + GUCAGGGACAGCGCCAUCAGCGGA 24 17990

HSV-UL54-2460 + GCGCCUUUCGCUCCGGGA 18 17991

HSV-UL54-2461 + GGCGCCUUUCGCUCCGGGA 19 17992

HSV-UL54-2462 + GGGCGCCUUUCGCUCCGGGA 20 17993

HSV-UL54-2463 + AGGGCGCCUUUCGCUCCGGGA 21 17994

HSV-UL54-2464 + GAGGGCGCCUU UCGCUCCGGGA 22 17995

HSV-UL54-2465 + AGAGGGCGCCU UUCGCUCCGGGA 23 17996

HSV-UL54-2466 + CAGAGGGCGCCUU UCGCUCCGGGA 24 17997

HSV-UL54-2467 + AUUUGCCGUGCACAUAUA 18 17998

HSV-UL54-2468 + UAUUUGCCGUGCACAUAUA 19 17999

HSV-UL54-1375 + GUAUUUGCCGUGCACAUAUA 20 16906

HSV-UL54-2469 + AGUAUUUGCCGUGCACAUAUA 21 18000

HSV-UL54-2470 + AAGUAUUUGCCGUGCACAUAUA 22 18001

HSV-UL54-2471 + GAAG UAUUUGCCGUGCACAUAUA 23 18002

HSV-UL54-2472 + AGAAGUAUUUGCCGUGCACAUAUA 24 18003

HSV-UL54-2473 + CGCUUGGGUGGCCAGCAC 18 18004

HSV-UL54-2474 + CCGCUUGGGUGGCCAGCAC 19 18005

HSV-UL54-420 + CCCGCUUGGGUGGCCAGCAC 20 6843

HSV-UL54-2475 + CCCCGCUUGGGUGGCCAGCAC 21 18006

HSV-UL54-2476 + CCCCCGCUUGGGUGGCCAGCAC 22 18007

HSV-UL54-2477 + CCCCCCGCUUGGGUGGCCAGCAC 23 18008

HSV-UL54-2478 + CCCCCCCGCUUGGGUGGCCAGCAC 24 18009 HSV-UL54-2479 + CCGUCCGUCCGCGCCGAC 18 18010

HSV-UL54-2480 + GCCGUCCGUCCGCGCCGAC 19 18011

HSV-UL54-2481 + GGCCGUCCGUCCGCGCCGAC 20 18012

HSV-UL54-2482 + GGGCCGUCCGUCCGCGCCGAC 21 18013

HSV-UL54-2483 + GGGGCCGUCCGUCCGCGCCGAC 22 18014

HSV-UL54-2484 + CGGGGCCGUCCGUCCGCGCCGAC 23 18015

HSV-UL54-2485 + CCGGGGCCGUCCGUCCGCGCCGAC 24 18016

HSV-UL54-2486 + UCAGCUCGCACACGCGAC 18 18017

HSV-UL54-2487 + G U CAGCU CG CACACG CG AC 19 18018

HSV-UL54-1379 + CGUCAGCUCGCACACGCGAC 20 16910

HSV-UL54-2488 + CCGUCAGCUCGCACACGCGAC 21 18019

HSV-UL54-2489 + GCCGUCAGCUCGCACACGCGAC 22 18020

HSV-UL54-2490 + GGCCGUCAGCUCGCACACGCGAC 23 18021

HSV-UL54-2491 + UGGCCGUCAGCUCGCACACGCGAC 24 18022

HSV-UL54-2492 + CGCCUUUCGCUCCGGGAC 18 18023

HSV-UL54-2493 + GCGCCUUUCGCUCCGGGAC 19 18024

HSV-UL54-1380 + GGCGCCUUUCGCUCCGGGAC 20 16911

HSV-UL54-2494 + GGGCGCCUUUCGCUCCGGGAC 21 18025

HSV-UL54-2495 + AGGGCGCCUUUCGCUCCGGGAC 22 18026

HSV-UL54-2496 + GAGGGCGCCUUUCGCUCCGGGAC 23 18027

HSV-UL54-2497 + AGAGGGCGCCUUUCGCUCCGGGAC 24 18028

HSV-UL54-2498 + AAAGGGGUCUUGCAUGAC 18 18029

HSV-UL54-2499 + CAAAGGGGUCUUGCAUGAC 19 18030

HSV-UL54-429 + CCAAAGGGGUCUUGCAUGAC 20 6852

HSV-UL54-2500 + GCCAAAGGGGUCUUGCAUGAC 21 18031

HSV-UL54-2501 + CGCCAAAGGGGUCUUGCAUGAC 22 18032

HSV-UL54-2502 + CCGCCAAAGGGGUCUUGCAUGAC 23 18033

HSV-UL54-2503 + CCCGCCAAAGGGGUCU UGCAUGAC 24 18034

HSV-UL54-2504 + CUCGUGGCCGCGGGCCCC 18 18035

HSV-UL54-2505 + CCUCGUGGCCGCGGGCCCC 19 18036

HSV-UL54-1386 + GCCUCGUGGCCGCGGGCCCC 20 16917

HSV-UL54-2506 + UGCCUCGUGGCCGCGGGCCCC 21 18037

HSV-UL54-2507 + GUGCCUCGUGGCCGCGGGCCCC 22 18038

HSV-UL54-2508 + CGUGCCUCGUGGCCGCGGGCCCC 23 18039

HSV-UL54-2509 + GCGUGCCUCGUGGCCGCGGGCCCC 24 18040

HSV-UL54-2510 + UGGCCAGCACGGGAGCCC 18 18041

HSV-UL54-2511 + GUGGCCAGCACGGGAGCCC 19 18042

HSV-UL54-1387 + G G U G G CCAG CACG G G AG CCC 20 16918

HSV-UL54-2512 + GGGUGGCCAGCACGGGAGCCC 21 18043

HSV-UL54-2513 + UGGGUGGCCAGCACGGGAGCCC 22 18044

HSV-UL54-2514 + UUGGG UGGCCAGCACGGGAGCCC 23 18045

HSV-UL54-2515 + CUUGGGUGGCCAGCACGGGAGCCC 24 18046

HSV-UL54-2516 + GACCCGCCAUGCAGGCCC 18 18047

HSV-UL54-2517 + AGACCCGCCAUGCAGGCCC 19 18048

HSV-UL54-2518 + G AG ACCCG CCAUGCAG G CCC 20 18049

HSV-UL54-2519 + UGAGACCCGCCAUGCAGGCCC 21 18050 HSV-UL54-2520 + AUGAGACCCGCCAUGCAGGCCC 22 18051

HSV-UL54-2521 + AAUGAGACCCGCCAUGCAGGCCC 23 18052

HSV-UL54-2522 + CAAUGAGACCCGCCAUGCAGGCCC 24 18053

HSV-UL54-2523 + CCUCGUGGCCGCGGGCCC 18 18054

HSV-UL54-2524 + GCCUCGUGGCCGCGGGCCC 19 18055

HSV-UL54-2525 + UGCCUCGUGGCCGCGGGCCC 20 18056

HSV-UL54-2526 + GUGCCUCGUGGCCGCGGGCCC 21 18057

HSV-UL54-2527 + CGUGCCUCGUGGCCGCGGGCCC 22 18058

HSV-UL54-2528 + GCGUGCCUCGUGGCCGCGGGCCC 23 18059

HSV-UL54-2529 + CGCGUGCCUCGUGGCCGCGGGCCC 24 18060

HSV-UL54-2530 + GAGCGACCAGGGUUUCCC 18 18061

HSV-UL54-2531 + UGAGCGACCAGGGUUUCCC 19 18062

HSV-UL54-1388 + GUGAGCGACCAGGGUUUCCC 20 16919

HSV-UL54-2532 + CGUGAGCGACCAGGGUUUCCC 21 18063

HSV-UL54-2533 + CCGUGAGCGACCAGGGUUUCCC 22 18064

HSV-UL54-2534 + GCCGUGAGCGACCAGGGUUUCCC 23 18065

HSV-UL54-2535 + GGCCGUGAGCGACCAGGGUUUCCC 24 18066

HSV-UL54-2536 + GUGGCCAGCACGGGAGCC 18 18067

HSV-UL54-2537 + GGUGGCCAGCACGGGAGCC 19 18068

HSV-UL54-2538 + GGGUGGCCAGCACGGGAGCC 20 18069

HSV-UL54-2539 + UGGGUGGCCAGCACGGGAGCC 21 18070

HSV-UL54-2540 + UUGGGUGGCCAGCACGGGAGCC 22 18071

HSV-UL54-2541 + CU UGGGUGGCCAGCACGGGAGCC 23 18072

HSV-UL54-2542 + GCUUGGGUGGCCAGCACGGGAGCC 24 18073

HSV-UL54-2543 + UGUCCGACAGGCGUCGCC 18 18074

HSV-UL54-2544 + AUGUCCGACAGGCGUCGCC 19 18075

HSV-UL54-997 + AAUGUCCGACAGGCGUCGCC 20 7320

HSV-UL54-2545 + UAAUGUCCGACAGGCGUCGCC 21 18076

HSV-UL54-2546 + UUAAUGUCCGACAGGCGUCGCC 22 18077

HSV-UL54-2547 + CUUAAUGUCCGACAGGCGUCGCC 23 18078

HSV-UL54-2548 + CCUUAAUGUCCGACAGGCGUCGCC 24 18079

HSV-UL54-2549 + GUCCAGCCCGCACAGGCC 18 18080

HSV-UL54-2550 + CGUCCAGCCCGCACAGGCC 19 18081

HSV-UL54-2551 + UCGUCCAGCCCGCACAGGCC 20 18082

HSV-UL54-2552 + GUCGUCCAGCCCGCACAGGCC 21 18083

HSV-UL54-2553 + GGUCGUCCAGCCCGCACAGGCC 22 18084

HSV-UL54-2554 + AGGUCGUCCAGCCCGCACAGGCC 23 18085

HSV-UL54-2555 + CAGGUCGUCCAGCCCGCACAGGCC 24 18086

HSV-UL54-2556 + GCGGUAGAGGCUCGGGCC 18 18087

HSV-UL54-2557 + UGCGGUAGAGGCUCGGGCC 19 18088

HSV-UL54-2558 + GUGCGGUAGAGGCUCGGGCC 20 18089

HSV-UL54-2559 + UGU GCGGUAGAGGCUCGGGCC 21 18090

HSV-UL54-2560 + AUG UGCGGUAGAGGCUCGGGCC 22 18091

HSV-UL54-2561 + AAUGUGCGGUAGAGGCUCGGGCC 23 18092

HSV-UL54-2562 + GAAUGUGCGGUAGAGGCUCGGGCC 24 18093

HSV-UL54-2563 + GGCGCGUGCCUCGUGGCC 18 18094 HSV-UL54-2564 + UGGCGCGUGCCUCGUGGCC 19 18095

HSV-UL54-2565 + CUGGCGCGUGCCUCGUGGCC 20 18096

HSV-UL54-2566 + CCUGGCGCGUGCCUCGUGGCC 21 18097

HSV-UL54-2567 + GCCUGGCGCGUGCCUCGUGGCC 22 18098

HSV-UL54-2568 + GGCCUGGCGCGUGCCUCGUGGCC 23 18099

HSV-UL54-2569 + GGGCCUGGCGCGUGCCUCGUGGCC 24 18100

HSV-UL54-2570 + CACGCCAGCGUCUCAUCC 18 18101

HSV-UL54-2571 + CCACGCCAGCGUCUCAUCC 19 18102

HSV-UL54-2572 + ACCACGCCAGCGUCUCAUCC 20 18103

HSV-UL54-2573 + CACCACGCCAGCGUCUCAUCC 21 18104

HSV-UL54-2574 + GCACCACGCCAGCGUCUCAUCC 22 18105

HSV-UL54-2575 + UGCACCACGCCAGCGUCUCAUCC 23 18106

HSV-UL54-2576 + U U G CACCACG CCAGCG U CU CAUCC 24 18107

HSV-UL54-2577 + GUUCCGAUGAUAGGGUCC 18 18108

HSV-UL54-2578 + CGUUCCGAUGAUAGGGUCC 19 18109

HSV-UL54-400 + CCGUUCCGAUGAUAGGGUCC 20 6823

HSV-UL54-2579 + GCCGUUCCGAUGAUAGGGUCC 21 18110

HSV-UL54-2580 + CGCCGUUCCGAUGAUAGGGUCC 22 18111

HSV-UL54-2581 + CCGCCGUUCCGAUGAUAGGGUCC 23 18112

HSV-UL54-2582 + GCCGCCGUUCCGAUGAUAGGGUCC 24 18113

HSV-UL54-2583 + UGAGCGACCAGGGUUUCC 18 18114

HSV-UL54-2584 + GUGAGCGACCAGGGUUUCC 19 18115

HSV-UL54-2585 + CGUGAGCGACCAGGGUUUCC 20 18116

HSV-UL54-2586 + CCGUGAGCGACCAGGGUUUCC 21 18117

HSV-UL54-2587 + GCCGUGAGCGACCAGGGUUUCC 22 18118

HSV-UL54-2588 + GGCCG UGAGCGACCAGGGUUUCC 23 18119

HSV-UL54-2589 + GGGCCGUGAGCGACCAGGGUUUCC 24 18120

HSV-UL54-2590 + CCCGCUUGGGUGGCCAGC 18 18121

HSV-UL54-2591 + CCCCGCUUGGGUGGCCAGC 19 18122

HSV-UL54-2592 + CCCCCGCUUGGGUGGCCAGC 20 18123

HSV-UL54-2593 + CCCCCCGCUUGGGUGGCCAGC 21 18124

HSV-UL54-2594 + CCCCCCCGCUUGGGUGGCCAGC 22 18125

HSV-UL54-2595 + ACCCCCCCGCUUGGGUGGCCAGC 23 18126

HSV-UL54-2596 + AACCCCCCCGCUUGGGUGGCCAGC 24 18127

HSV-UL54-2597 + CGUCUCAUCCGCGGACGC 18 18128

HSV-UL54-2598 + GCGUCUCAUCCGCGGACGC 19 18129

HSV-UL54-2599 + AGCGUCUCAUCCGCGGACGC 20 18130

HSV-UL54-2600 + CAGCGUCUCAUCCGCGGACGC 21 18131

HSV-UL54-2601 + CCAGCGUCUCAUCCGCGGACGC 22 18132

HSV-UL54-2602 + GCCAGCGUCUCAUCCGCGGACGC 23 18133

HSV-UL54-2603 + CGCCAGCGUCUCAUCCGCGGACGC 24 18134

HSV-UL54-2604 + UAUGGAUCGCAGAACCGC 18 18135

HSV-UL54-2605 + AUAUGGAUCGCAGAACCGC 19 18136

HSV-UL54-2606 + GAUAUGGAUCGCAGAACCGC 20 18137

HSV-UL54-2607 + GGAUAUGGAUCGCAGAACCGC 21 18138

HSV-UL54-2608 + CGG AUAUGGAUCGCAGAACCGC 22 18139 HSV-UL54-2609 + UCGGAUAUGGAUCGCAGAACCGC 23 18140

HSV-UL54-2610 + CUCGGAUAUGGAUCGCAGAACCGC 24 18141

HSV-UL54-2611 + CCUCGCCGUGGGUGGCGC 18 18142

HSV-UL54-2612 + GCCUCGCCGUGGGUGGCGC 19 18143

HSV-UL54-455 + CGCCUCGCCGUGGGUGGCGC 20 6878

HSV-UL54-2613 + GCGCCUCGCCGUGGGUGGCGC 21 18144

HSV-UL54-2614 + CGCGCCUCGCCGUGGGUGGCGC 22 18145

HSV-UL54-2615 + CCGCGCCUCGCCGUGGGUGGCGC 23 18146

HSV-UL54-2616 + GCCGCGCCUCGCCGUGGGUGGCGC 24 18147

HSV-UL54-2617 + CGAAUGUGCGGUAGAGGC 18 18148

HSV-UL54-2618 + GCGAAUGUGCGGUAGAGGC 19 18149

HSV-UL54-2619 + UGCGAAUGUGCGGUAGAGGC 20 18150

HSV-UL54-2620 + CUGCGAAUGUGCGGUAGAGGC 21 18151

HSV-UL54-2621 + GCUGCGAAUGUGCGGUAGAGGC 22 18152

HSV-UL54-2622 + GGCUGCGAAUGUGCGGUAGAGGC 23 18153

HSV-UL54-2623 + UGGCUGCGAAUGUGCGGUAGAGGC 24

18154

HSV-UL54-2624 + CCAGGGGCUGUUCGCGGC 18 18155

HSV-UL54-2625 + CCCAGGGGCUGUUCGCGGC 19 18156

HSV-UL54-1397 + GCCCAGGGGCUGUUCGCGGC 20 16928

HSV-UL54-2626 + AGCCCAGGGGCUGUUCGCGGC 21 18157

HSV-UL54-2627 + GAGCCCAGGGGCUGUUCGCGGC 22 18158

HSV-UL54-2628 + GGAGCCCAGGGGCUGUUCGCGGC 23 18159

HSV-UL54-2629 + GGGAGCCCAGGGGCUGUUCGCGGC 24 18160

HSV-UL54-2630 + GCGCGGCCGUCCGCGUGC 18 18161

HSV-UL54-2631 + GGCGCGGCCGUCCGCGUGC 19 18162

HSV-UL54-440 + GGGCGCGGCCGUCCGCGUGC 20 6863

HSV-UL54-2632 + GGGGCGCGGCCGUCCGCGUGC 21 18163

HSV-UL54-2633 + CGGGGCGCGGCCGUCCGCGUGC 22 18164

HSV-UL54-2634 + CCGGGGCGCGGCCGUCCGCGUGC 23 18165

HSV-UL54-2635 + ACCGGGGCGCGGCCGUCCGCGUGC 24 18166

HSV-UL54-2636 + AGAGGGCGCCUUUCGCUC 18 18167

HSV-UL54-2637 + CAGAGGGCGCCUUUCGCUC 19 18168

HSV-UL54-1400 + GCAGAGGGCGCCUUUCGCUC 20 16931

HSV-UL54-2638 + GGCAGAGGGCGCCU UUCGCUC 21 18169

HSV-UL54-2639 + CGGCAGAGGGCGCCUUUCGCUC 22 18170

HSV-UL54-2640 + UCGGCAGAGGGCGCCU UUCGCUC 23 18171

HSV-UL54-2641 + GUCGGCAGAGGGCGCCUUUCGCUC 24 18172

HSV-UL54-2642 + CGUUCCGAUGAUAGGGUC 18 18173

HSV-UL54-2643 + CCGUUCCGAUGAUAGGGUC 19 18174

HSV-UL54-2644 + GCCGUUCCGAUGAUAGGGUC 20 18175

HSV-UL54-2645 + CGCCGUUCCGAUGAUAGGGUC 21 18176

HSV-UL54-2646 + CCGCCGUUCCGAUGAUAGGGUC 22 18177

HSV-UL54-2647 + GCCGCCGUUCCGAUGAUAGGGUC 23 18178

HSV-UL54-2648 + GGCCGCCGUUCCGAUGAUAGGGUC 24 18179

HSV-UL54-2649 + UCCGCGUGCGGGGGGGUC 18 18180 HSV-UL54-2650 + GUCCGCGUGCGGGGGGGUC 19 18181

HSV-UL54-1402 + CGUCCGCGUGCGGGGGGGUC 20 16933

HSV-UL54-2651 + CCGUCCGCGUGCGGGGGGGUC 21 18182

HSV-UL54-2652 + GCCGUCCGCGUGCGGGGGGGUC 22 18183

HSV-UL54-2653 + GGCCGUCCGCGUGCGGGGGGGUC 23 18184

HSV-UL54-2654 + CGGCCGUCCGCGUGCGGGGGGGUC 24 18185

HSV-UL54-1663 + CUUGGU UGUGGGCGUUUC 18 17194

HSV-UL54-1664 + CCUUGGUUGUGGGCGUUUC 19 17195

HSV-UL54-1237 + CCCUUGGUUGUGGGCGUUUC 20 16768

HSV-UL54-1665 + CCCCUUGGUUGUGGGCGUUUC 21 17196

HSV-UL54-1666 + CCCCCUUGGUUGUGGGCGUUUC 22 17197

HSV-UL54-1667 + ACCCCCUUGGUUGUGGGCGUUUC 23 17198

HSV-UL54-1668 + GACCCCCUUGGUUGUGGGCGUUUC 24 17199

HSV-UL54-2655 + UAAAAUAGGGAGUUGCAG 18 18186

HSV-UL54-2656 + CUAAAAUAGGGAGUUGCAG 19 18187

HSV-UL54-2657 + CCUAAAAUAGGGAGUUGCAG 20 18188

HSV-UL54-2658 + GCCUAAAAUAGGGAGUUGCAG 21 18189

HSV-UL54-2659 + UGCCUAAAAUAGGGAGUUGCAG 22 18190

HSV-UL54-2660 + UUGCCUAAAAUAGGGAGUUGCAG 23 18191

HSV-UL54-2661 + CUUGCCUAAAAUAGGGAGUUGCAG 24 18192

HSV-UL54-2662 + CAGGGACAGCGCCAUCAG 18 18193

HSV-UL54-2663 + UCAGGGACAGCGCCAUCAG 19 18194

HSV-UL54-1408 + GUCAGGGACAGCG CCAU CAG 20 16939

HSV-UL54-2664 + GGUCAGGGACAGCGCCAUCAG 21 18195

HSV-UL54-2665 + GGG UCAGGGACAGCGCCAUCAG 22 18196

HSV-UL54-2666 + GGGGU CAGGGACAGCGCCAUCAG 23 18197

HSV-UL54-2667 + GGGGGUCAGGGACAGCGCCAUCAG 24 18198

HSV-UL54-2668 + CUCGACGCGGUUGGCGAG 18 18199

HSV-UL54-2669 + GCUCGACGCGGUUGGCGAG 19 18200

HSV-UL54-388 + CGCUCGACGCGGUUGGCGAG 20 6811

HSV-UL54-2670 + GCGCUCGACGCGGUUGGCGAG 21 18201

HSV-UL54-2671 + CGCGCUCGACGCGGUUGGCGAG 22 18202

HSV-UL54-2672 + CCGCGCUCGACGCGGUUGGCGAG 23 18203

HSV-UL54-2673 + GCCGCGCUCGACGCGGUUGGCGAG 24 18204

HSV-UL54-2674 + ACAGCGCCAUCAGCGGAG 18 18205

HSV-UL54-2675 + GACAGCGCCAUCAGCGGAG 19 18206

HSV-UL54-1409 + GGACAGCGCCAUCAGCGGAG 20 16940

HSV-UL54-2676 + G G G ACAG CG CCAU CAG CG G AG 21 18207

HSV-UL54-2677 + AGGGACAGCGCCAUCAGCGGAG 22 18208

HSV-UL54-2678 + CAG G G ACAG CG CCAU CAG CG GAG 23 18209

HSV-UL54-2679 + UCAGGGACAGCGCCAUCAGCGGAG 24 18210

HSV-UL54-2680 + CGCAGAACCGCCCGAACG 18 18211

HSV-UL54-2681 + U CG CAG AACCG CCCGAACG 19 18212

HSV-UL54-2682 + AUCGCAGAACCGCCCGAACG 20 18213

HSV-UL54-2683 + GAU CGCAGAACCGCCCGAACG 21 18214

HSV-UL54-2684 + GGAUCGCAGAACCGCCCGAACG 22 18215 HSV-UL54-2685 + UGGAUCGCAGAACCGCCCGAACG 23 18216

HSV-UL54-2686 + AUGGAUCGCAGAACCGCCCGAACG 24 18217

HSV-UL54-2687 + CGCGGGCCCCCGGAGACG 18 18218

HSV-UL54-2688 + CCGCGGGCCCCCGGAGACG 19 18219

HSV-UL54-2689 + GCCGCGGGCCCCCGGAGACG 20 18220

HSV-UL54-2690 + GGCCGCGGGCCCCCGGAGACG 21 18221

HSV-UL54-2691 + UGGCCGCGGGCCCCCGGAGACG 22 18222

HSV-UL54-2692 + GUGGCCGCGGGCCCCCGGAGACG 23 18223

HSV-UL54-2693 + CGUGGCCGCGGGCCCCCGGAGACG 24 18224

HSV-UL54-1681 + GUU UCUGGAGACGCGCCG 18 17212

HSV-UL54-1682 + CGUUUCUGGAGACGCGCCG 19 17213

HSV-UL54-1683 + GCGUUUCUGGAGACGCGCCG 20 17214

HSV-UL54-1684 + GGCGUU UCUGGAGACGCGCCG 21 17215

HSV-UL54-1685 + GGGCGUUUCUGGAGACGCGCCG 22 17216

HSV-UL54-1686 + UGGGCGU UUCUGGAGACGCGCCG 23 17217

HSV-UL54-1687 + GUGGGCGUUUCUGGAGACGCGCCG 24 17218

HSV-UL54-2694 + GGGACAGCGCCAUCAGCG 18 18225

HSV-UL54-2695 + AGGGACAGCGCCAUCAGCG 19 18226

HSV-UL54-2696 + CAGGGACAGCGCCAUCAGCG 20 18227

HSV-UL54-2697 + UCAGGGACAGCGCCAUCAGCG 21 18228

HSV-UL54-2698 + GUCAGGGACAGCGCCAUCAGCG 22 18229

HSV-UL54-2699 + GGUCAGGGACAGCGCCAUCAGCG 23 18230

HSV-UL54-2700 + GGGUCAGGGACAGCGCCAUCAGCG 24 18231

HSV-UL54-2701 + GCCCAGGGGCUGUUCGCG 18 18232

HSV-UL54-2702 + AGCCCAGGGGCUGUUCGCG 19 18233

HSV-UL54-2703 + GAGCCCAGGGGCUGUUCGCG 20 18234

HSV-UL54-2704 + GGAGCCCAGGGGCUGUUCGCG 21 18235

HSV-UL54-2705 + GGGAGCCCAGGGGCUGUUCGCG 22 18236

HSV-UL54-2706 + CGGG AGCCCAGGGGCUGUUCGCG 23 18237

HSV-UL54-2707 + ACGGGAGCCCAGGGGCUGUUCGCG 24 18238

HSV-UL54-2708 + GCCUCGCCGUGGGUGGCG 18 18239

HSV-UL54-2709 + CGCCUCGCCGUGGGUGGCG 19 18240

HSV-UL54-2710 + GCGCCUCGCCGUGGGUGGCG 20 18241

HSV-UL54-2711 + CGCGCCUCGCCGUGGGUGGCG 21 18242

HSV-UL54-2712 + CCGCGCCUCGCCGUGGGUGGCG 22 18243

HSV-UL54-2713 + GCCGCGCCUCGCCGUGGGUGGCG 23 18244

HSV-UL54-2714 + CGCCGCGCCUCGCCGUGGGUGGCG 24 18245

HSV-UL54-2715 + CGCGGCCGUCCGCGUGCG 18 18246

HSV-UL54-2716 + GCGCGGCCGUCCGCGUGCG 19 18247

HSV-UL54-1425 + GGCGCGGCCGUCCGCGUGCG 20 16956

HSV-UL54-2717 + GGGCGCGGCCGUCCGCGUGCG 21 18248

HSV-UL54-2718 + GGGGCGCGGCCGUCCGCGUGCG 22 18249

HSV-UL54-2719 + CGGGGCGCGGCCGUCCGCGUGCG 23 18250

HSV-UL54-2720 + CCGGGGCGCGGCCGUCCGCGUGCG 24 18251

HSV-UL54-2721 + GCGCUCGGAUAUGGAUCG 18 18252

HSV-UL54-2722 + CGCGCUCGGAUAUGGAUCG 19 18253 HSV-UL54-2723 + GCGCGCUCGGAUAUGGAUCG 20 18254

HSV-UL54-2724 + CGCGCGCUCGGAUAUGGAUCG 21 18255

HSV-UL54-2725 + CCGCGCGCUCGGAUAUGGAUCG 22 18256

HSV-UL54-2726 + GCCGCGCGCUCGGAUAUGGAUCG 23 18257

HSV-UL54-2727 + CGCCGCGCGCUCGGAUAUGGAUCG 24 18258

HSV-UL54-2728 + GCGGACGCCAGGGCCUCG 18 18259

HSV-UL54-2729 + CGCGGACGCCAGGGCCUCG 19 18260

HSV-UL54-1008 + CCGCGGACGCCAGGGCCUCG 20 7329

HSV-UL54-2730 + UCCGCGGACGCCAGGGCCUCG 21 18261

HSV-UL54-2731 + AUCCGCGGACGCCAGGGCCUCG 22 18262

HSV-UL54-2732 + CAUCCGCGGACGCCAGGGCCUCG 23 18263

HSV-UL54-2733 + UCAUCCGCGGACGCCAGGGCCUCG 24 18264

HSV-UL54-2734 + CAGCGCCAUCAGCGGAGG 18 18265

HSV-UL54-2735 + ACAGCGCCAUCAGCGGAGG 19 18266

HSV-UL54-1431 + GACAGCGCCAUCAGCGGAGG 20 16962

HSV-UL54-2736 + GGACAGCGCCAUCAGCGGAGG 21 18267

HSV-UL54-2737 + GGGACAGCGCCAUCAGCGGAGG 22 18268

HSV-UL54-2738 + AGGGACAGCGCCAUCAGCGGAGG 23 18269

HSV-UL54-2739 + CAGGGACAGCGCCAUCAGCGGAGG 24 18270

HSV-UL54-2740 + GGACAGCGCCAUCAGCGG 18 18271

HSV-UL54-2741 + GGGACAGCGCCAUCAGCGG 19 18272

HSV-UL54-1434 + AGGGACAGCGCCAUCAGCGG 20 16965

HSV-UL54-2742 + CAGGGACAGCG CCAU CAG CG G 21 18273

HSV-UL54-2743 + UCAGGGACAGCGCCAUCAGCGG 22 18274

HSV-UL54-2744 + GUCAGGGACAGCGCCAUCAGCGG 23 18275

HSV-UL54-2745 + GGUCAGGGACAGCGCCAUCAGCGG 24 18276

HSV-UL54-2746 + CCCAGGGGCUGUUCGCGG 18 18277

HSV-UL54-2747 + GCCCAGGGGCUGU UCGCGG 19 18278

HSV-UL54-1435 + AGCCCAGGGGCUGUUCGCGG 20 16966

HSV-UL54-2748 + GAGCCCAGGGGCUGUUCGCGG 21 18279

HSV-UL54-2749 + GGAGCCCAGGGGCUGUUCGCGG 22 18280

HSV-UL54-2750 + GGG AGCCCAGGGGCUGUUCGCGG 23 18281

HSV-UL54-2751 + CGGGAGCCCAGGGGCUGUUCGCGG 24 18282

HSV-UL54-2752 + UUGGCUGCGAAUGUGCGG 18 18283

HSV-UL54-2753 + GUUGGCUGCGAAUGUGCGG 19 18284

HSV-UL54-2754 + GGUUGGCUGCGAAUGUGCGG 20 18285

HSV-UL54-2755 + GGGUUGGCUGCGAAUGUGCGG 21 18286

HSV-UL54-2756 + CGGG UUGGCUGCGAAUGUGCGG 22 18287

HSV-UL54-2757 + GCGGGUUGGCUGCGAAUGUGCGG 23 18288

HSV-UL54-2758 + CGCGGGUUGGCUGCGAAUGUGCGG 24

18289

HSV-UL54-2759 + CGGGGUCGAUGGUGUCGG 18 18290

HSV-UL54-2760 + GCGGGGUCGAUGGUGUCGG 19 18291

HSV-UL54-2761 + GGCGGGGUCGAUGGUGUCGG 20 18292

HSV-UL54-2762 + CGGCGGGGUCGAUGGUGUCGG 21 18293

HSV-UL54-2763 + ACGGCGGGGUCGAUGGUGUCGG 22 18294 HSV-UL54-2764 + AACGGCGGGGUCGAUGGUGUCGG 23 18295

HSV-UL54-2765 + GAACGGCGGGGUCGAUGGUGUCGG 24

18296

HSV-UL54-2766 + AUGAUAGGGUCCUGGGGG 18 18297

HSV-UL54-2767 + GAUGAUAGGGUCCUGGGGG 19 18298

HSV-UL54-1442 + CGAUGAUAGGGUCCUGGGGG 20 16973

HSV-UL54-2768 + CCGAUGAUAGGGUCCUGGGGG 21 18299

HSV-UL54-2769 + UCCGAUGAUAGGGUCCUGGGGG 22 18300

HSV-UL54-2770 + UUCCGAUGAUAGGGUCCUGGGGG 23 18301

HSV-UL54-2771 + GUUCCGAUGAUAGGGUCCUGGGGG 24

18302

HSV-UL54-2772 + GAUGAUAGGGUCCUGGGG 18 18303

HSV-UL54-2773 + CGAUGAUAGGGUCCUGGGG 19 18304

HSV-UL54-2774 + CCGAUGAUAGGGUCCUGGGG 20 18305

HSV-UL54-2775 + UCCGAUGAUAGGGUCCUGGGG 21 18306

HSV-UL54-2776 + UUCCGAUGAUAGGGUCCUGGGG 22 18307

HSV-UL54-2777 + GU UCCGAUGAUAGGGUCCUGGGG 23 18308

HSV-UL54-2778 + CGUUCCGAUGAUAGGGUCCUGGGG 24 18309

HSV-UL54-2779 + GGCCUUCAGGUAGCACUG 18 18310

HSV-UL54-2780 + GGGCCUUCAGGUAGCACUG 19 18311

HSV-UL54-397 + CGGGCCUUCAGGUAGCACUG 20 6820

HSV-UL54-2781 + UCGGGCCUUCAGGUAGCACUG 21 18312

HSV-UL54-2782 + CUCGGGCCUUCAGGUAGCACUG 22 18313

HSV-UL54-2783 + CCUCGGGCCUUCAGGUAGCACUG 23 18314

HSV-UL54-2784 + GCCUCGGGCCUUCAGGUAGCACUG 24 18315

HSV-UL54-2785 + GGCGCGGCCGUCCGCGUG 18 18316

HSV-UL54-2786 + GGGCGCGGCCGUCCGCGUG 19 18317

HSV-UL54-1447 + GGGGCGCGGCCGUCCGCGUG 20 16978

HSV-UL54-2787 + CGGGGCGCGGCCGUCCGCGUG 21 18318

HSV-UL54-2788 + CCGGGGCGCGGCCGUCCGCGUG 22 18319

HSV-UL54-2789 + ACCGGGGCGCGGCCGUCCGCGUG 23 18320

HSV-UL54-2790 + GACCGGGGCGCGGCCGUCCGCGUG 24 18321

HSV-UL54-2791 + AAGGGGCGCAGGCGCGUG 18 18322

HSV-UL54-2792 + AAAGGGGCGCAGGCGCGUG 19 18323

HSV-UL54-2793 + GAAAGGGGCGCAGGCGCGUG 20 18324

HSV-UL54-2794 + AGAAAGGGGCGCAGGCGCGUG 21 18325

HSV-UL54-2795 + CAG AAAGGGGCGCAGGCGCGUG 22 18326

HSV-UL54-2796 + GCAGAAAGGGGCGCAGGCGCGUG 23 18327

HSV-UL54-2797 + UGCAGAAAGGGGCGCAGGCGCGUG 24 18328

HSV-UL54-1485 + GCCGGCGGGGUU UUGGUG 18 17016

HSV-UL54-1528 + CGCCGGCGGGGUUUUGGUG 19 17059

HSV-UL54-23 + GCGCCGGCGGGGUUUUGGUG 20 6446

HSV-UL54-1529 + CGCGCCGGCGGGGUUUUGGUG 21 17060

HSV-UL54-1530 + ACGCGCCGGCGGGGUUUUGGUG 22 17061

HSV-UL54-1486 + GACGCGCCGGCGGGGUUUUGGUG 23 17017 HSV-UL54-1531 + AGACGCGCCGGCGGGGUUUUGGUG 24

17062

HSV-UL54-2798 + CCGCGCUCGACGCGGUUG 18 18329

HSV-UL54-2799 + GCCGCGCUCGACGCGGUUG 19 18330

HSV-UL54-2800 + CGCCGCGCUCGACGCGGUUG 20 18331

HSV-UL54-2801 + ACGCCGCGCUCGACGCGGUUG 21 18332

HSV-UL54-2802 + CACGCCGCGCUCGACGCGGUUG 22 18333

HSV-UL54-2803 + ACACGCCGCGCUCGACGCGGUUG 23 18334

HSV-UL54-2804 + GACACGCCGCGCUCGACGCGGUUG 24 18335

HSV-UL54-2805 + UUUAUUCUUGCCUAAAAU 18 18336

HSV-UL54-2806 + GUUUAUUCUUGCCUAAAAU 19 18337

HSV-UL54-1449 + UGUUUAUUCUUGCCUAAAAU 20 16980

HSV-UL54-2807 + AUGUUUAUUCUUGCCUAAAAU 21 18338

HSV-UL54-2808 + UAUGUUUAUUCUUGCCUAAAAU 22 18339

HSV-UL54-2809 + AUAUGUUUAUUCUUGCCUAAAAU 23 18340

HSV-UL54-2810 + AAUAUGUUUAUUCUUGCCUAAAAU 24 18341

HSV-UL54-2811 + UAU UUGCCGUGCACAUAU 18 18342

HSV-UL54-2812 + GUAUUUGCCGUGCACAUAU 19 18343

HSV-UL54-2813 + AGUAUUUGCCGUGCACAUAU 20 18344

HSV-UL54-2814 + AAGUAUUUGCCGUGCACAUAU 21 18345

HSV-UL54-2815 + GAAGUAUUUGCCGUGCACAUAU 22 18346

HSV-UL54-2816 + AGAAGUAUUUGCCGUGCACAUAU 23 18347

HSV-UL54-2817 + UAGAAGUAUUUGCCGUGCACAUAU 24 18348

HSV-UL54-2818 + UUCCGAUGAUAGGGUCCU 18 18349

HSV-UL54-2819 + GUUCCGAUGAUAGGGUCCU 19 18350

HSV-UL54-1453 + CGUUCCGAUGAUAGGGUCCU 20 16984

HSV-UL54-2820 + CCGUUCCGAUGAUAGGGUCCU 21 18351

HSV-UL54-2821 + GCCGUUCCGAUGAUAGGGUCCU 22 18352

HSV-UL54-2822 + CGCCGUUCCGAUGAUAGGGUCCU 23 18353

HSV-UL54-2823 + CCGCCGUUCCGAUGAUAGGGUCCU 24 18354

HSV-UL54-2824 + CAGAGGGCGCCUUUCGCU 18 18355

HSV-UL54-2825 + GCAGAGGGCGCCU UUCGCU 19 18356

HSV-UL54-2826 + GGCAGAGGGCGCCUUUCGCU 20 18357

HSV-UL54-2827 + CGGCAGAGGGCGCCUUUCGCU 21 18358

HSV-UL54-2828 + UCGGCAGAGGGCGCCUUUCGCU 22 18359

HSV-UL54-2829 + GUCGGCAGAGGGCGCCUUUCGCU 23 18360

HSV-UL54-2830 + UGUCGGCAGAGGGCGCCUUUCGCU 24 18361

HSV-UL54-2831 + GGGCGCGGCCGUCCGCGU 18 18362

HSV-UL54-2832 + GGGGCGCGGCCGUCCGCGU 19 18363

HSV-UL54-2833 + CGGGGCGCGGCCGUCCGCGU 20 18364

HSV-UL54-2834 + CCGGGGCGCGGCCGUCCGCGU 21 18365

HSV-UL54-2835 + ACCGGGGCGCGGCCGUCCGCGU 22 18366

HSV-UL54-2836 + GACCGGGGCGCGGCCGUCCGCGU 23 18367

HSV-UL54-2837 + GGACCGGGGCGCGGCCGUCCGCGU 24 18368

HSV-UL54-2838 + GUCCGCGUGCGGGGGGGU 18 18369

HSV-UL54-2839 + CGUCCGCGUGCGGGGGGGU 19 18370 HSV-UL54-2840 + CCGUCCGCGUGCGGGGGGGU 20 18371

HSV-UL54-2841 + GCCGUCCGCGUGCGGGGGGGU 21 18372

HSV-UL54-2842 + GGCCGUCCGCGUGCGGGGGGGU 22 18373

HSV-UL54-2843 + CGGCCGUCCGCGUGCGGGGGGGU 23 18374

HSV-UL54-2844 + GCGGCCGUCCGCGUGCGGGGGGGU 24 18375

HSV-UL54-1836 + CGCCGGCGGGGUUU UGGU 18 17367

HSV-UL54-1837 + GCGCCGGCGGGGUUUUGGU 19 17368

HSV-UL54-1838 + CGCGCCGGCGGGGUUUUGGU 20 17369

HSV-UL54-1839 + ACGCGCCGGCGGGGUU UUGGU 21 17370

HSV-UL54-1840 + GACGCGCCGGCGGGGUUUUGGU 22 17371

HSV-UL54-1841 + AGACGCGCCGGCGGGGU UUUGGU 23 17372

HSV-UL54-1842 + GAGACGCGCCGGCGGGGUUUUGGU 24

17373

HSV-UL54-2845 + GUGGCGACCCCCUUGGUU 18 18376

HSV-UL54-2846 + GGUGGCGACCCCCUUGGUU 19 18377

HSV-UL54-2847 + GGGUGGCGACCCCCUUGGUU 20 18378

HSV-UL54-2848 + GGGGUGGCGACCCCCUUGGUU 21 18379

HSV-UL54-2849 + CGGGGUGGCGACCCCCUUGGUU 22 18380

HSV-UL54-2850 + GCGGGGUGGCGACCCCCUUGGUU 23 18381

HSV-UL54-2851 + CGCGGGGUGGCGACCCCCUUGGU U 24 18382

HSV-UL54-1855 + CCUUGGU UGUGGGCGUUU 18 17386

HSV-UL54-1856 + CCCUUGGUUGUGGGCGUUU 19 17387

HSV-UL54-1857 + CCCCUUGGUUGUGGGCGUUU 20 17388

HSV-UL54-1858 + CCCCCUUGGUUGUGGGCGUUU 21 17389

HSV-UL54-1859 + ACCCCCUUGGUUGUGGGCGUUU 22 17390

HSV-UL54-1860 + GACCCCCUUGGUUGUGGGCGUUU 23 17391

HSV-UL54-1861 + CGACCCCCUUGGUUGUGGGCGUUU 24 17392

HSV-UL54-2852 - CCCGUGCUGGCCACCCAA 18 18383

HSV-UL54-2853 - UCCCGUGCUGGCCACCCAA 19 18384

HSV-UL54-933 - CUCCCGUGCUGGCCACCCAA 20 7280

HSV-UL54-2854 - GCUCCCGUGCUGGCCACCCAA 21 18385

HSV-UL54-2855 - GGCUCCCGUGCUGGCCACCCAA 22 18386

HSV-UL54-2856 - GGGCUCCCGUGCUGGCCACCCAA 23 18387

HSV-UL54-2857 - UGGGCUCCCGUGCUGGCCACCCAA 24 18388

HSV-UL54-1874 - CAGAAACGCCCACAACCA 18 17405

HSV-UL54-1875 - CCAG AAACG CCCACAACCA 19 17406

HSV-UL54-1216 - UCCAGAAACGCCCACAACCA 20 16747

HSV-UL54-1876 - CUCCAGAAACGCCCACAACCA 21 17407

HSV-UL54-1877 - UCUCCAGAAACGCCCACAACCA 22 17408

HSV-UL54-1878 - GUCUCCAGAAACGCCCACAACCA 23 17409

HSV-UL54-1879 - CG U CU CCAG AAACG CCCACAACCA 24 17410

HSV-UL54-2858 - CGGCGGUCGAGCGCAUCA 18 18389

HSV-UL54-2859 - GCGGCGGUCGAGCGCAUCA 19 18390

HSV-UL54-927 - CGCGGCGGUCGAGCGCAUCA 20 7275

HSV-UL54-2860 - GCGCGGCGGUCGAGCGCAUCA 21 18391

HSV-UL54-2861 - CGCGCGGCGGUCGAGCGCAUCA 22 18392 HSV-UL54-2862 - GCGCGCGGCGGUCGAGCGCAUCA 23 18393

HSV-UL54-2863 - AGCGCGCGGCGGUCGAGCGCAUCA 24 18394

HSV-UL54-2864 - GCCCCCAGGACCCUAUCA 18 18395

HSV-UL54-2865 - CGCCCCCAGGACCCUAUCA 19 18396

HSV-UL54-951 - CCGCCCCCAGGACCCUAUCA 20 7290

HSV-UL54-2866 - UCCGCCCCCAGGACCCUAUCA 21 18397

HSV-UL54-2867 - CUCCGCCCCCAGGACCCUAUCA 22 18398

HSV-UL54-2868 - GCUCCGCCCCCAGGACCCUAUCA 23 18399

HSV-UL54-2869 - CGCUCCGCCCCCAGGACCCUAUCA 24 18400

HSV-UL54-2870 - UGCGCCAGGAAAAUCUCA 18 18401

HSV-UL54-2871 - CUGCGCCAGGAAAAUCUCA 19 18402

HSV-UL54-947 - GCUGCGCCAGGAAAAUCUCA 20 7287

HSV-UL54-2872 - UGCUGCGCCAGGAAAAUCUCA 21 18403

HSV-UL54-2873 - GUGCUGCGCCAGGAAAAUCUCA 22 18404

HSV-UL54-2874 - CGUGCUGCGCCAGG AAAAU CU CA 23 18405

HSV-UL54-2875 - GCGUGCUGCGCCAGGAAAAUCUCA 24 18406

HSV-UL54-2876 - CCUGCAUGGCGGGUCUCA 18 18407

HSV-UL54-2877 - GCCUGCAUGGCGGGUCUCA 19 18408

HSV-UL54-974 - GGCCUGCAUGGCGGGUCUCA 20 7305

HSV-UL54-2878 - GGGCCUGCAUGGCGGGUCUCA 21 18409

HSV-UL54-2879 - GGGGCCUGCAUGGCGGGUCUCA 22 18410

HSV-UL54-2880 - GGGGGCCUGCAUGGCGGGUCUCA 23 18411

HSV-UL54-2881 - CGGGGGCCUGCAUGGCGGGUCUCA 24 18412

HSV-UL54-2882 - CCGCGCCCCGGUCCCGGA 18 18413

HSV-UL54-2883 - GCCGCGCCCCGGUCCCGGA 19 18414

HSV-UL54-923 - GGCCGCGCCCCGGUCCCGGA 20 7271

HSV-UL54-2884 - CGGCCGCGCCCCGGUCCCGGA 21 18415

HSV-UL54-2885 - ACGGCCGCGCCCCGGUCCCGGA 22 18416

HSV-UL54-2886 - GACGGCCGCGCCCCGGUCCCGGA 23 18417

HSV-UL54-2887 - GGACGGCCGCGCCCCGGUCCCGGA 24 18418

HSV-UL54-2888 - GGCGAGGCGCGGCGCGGA 18 18419

HSV-UL54-2889 - CGGCGAGGCGCGGCGCGGA 19 18420

HSV-UL54-283 - ACGGCGAGGCGCGGCGCGGA 20 6706

HSV-UL54-2890 - CACGGCGAGGCGCGGCGCGGA 21 18421

HSV-UL54-2891 - CCACGGCGAGGCGCGGCGCGGA 22 18422

HSV-UL54-2892 - CCCACGGCGAGGCGCGGCGCGGA 23 18423

HSV-UL54-2893 - ACCCACGGCGAGGCGCGGCGCGGA 24 18424

HSV-UL54-2894 - GCGGGUCUCAUUGAAAUA 18 18425

HSV-UL54-2895 - GGCGGGUCUCAUUGAAAUA 19 18426

HSV-UL54-975 - UGGCGGGUCUCAUUGAAAUA 20 7306

HSV-UL54-2896 - AUGGCGGGUCUCAUUGAAAUA 21 18427

HSV-UL54-2897 - CAUGGCGGGUCUCAUUGAAAUA 22 18428

HSV-UL54-2898 - GCAUGGCGGGUCUCAUUGAAAUA 23 18429

HSV-UL54-2899 - UGCAUGGCGGGUCUCAUUGAAAUA 24 18430

HSV-UL54-2900 - UCCCUGACCCCCCCGCAC 18 18431

HSV-UL54-2901 - GUCCCUGACCCCCCCGCAC 19 18432 HSV-UL54-920 - UGUCCCUGACCCCCCCGCAC 20 7269

HSV-UL54-2902 - CUGUCCCUGACCCCCCCGCAC 21 18433

HSV-UL54-2903 - GCUGUCCCUGACCCCCCCGCAC 22 18434

HSV-UL54-2904 - CGCUGUCCCUGACCCCCCCGCAC 23 18435

HSV-UL54-2905 - GCGCUGUCCCUGACCCCCCCGCAC 24 18436

HSV-UL54-2906 - CCCUUUCUGCAGUGCUAC 18 18437

HSV-UL54-2907 - CCCCUUUCUGCAGUGCUAC 19 18438

HSV-UL54-955 - GCCCCUUUCUGCAGUGCUAC 20 7292

HSV-UL54-2908 - CGCCCCUUUCUGCAGUGCUAC 21 18439

HSV-UL54-2909 - GCGCCCCUUUCUGCAGUGCUAC 22 18440

HSV-UL54-2910 - UGCGCCCCUUUCUGCAGUGCUAC 23 18441

HSV-UL54-2911 - CUGCGCCCCUUUCUGCAGUGCUAC 24 18442

HSV-UL54-1934 - CCAGAAACGCCCACAACC 18 17465

HSV-UL54-1935 - UCCAGAAACGCCCACAACC 19 17466

HSV-UL54-1936 - CUCCAGAAACGCCCACAACC 20 17467

HSV-UL54-1937 - U CU CCAGAAACGCCCACAACC 21 17468

HSV-UL54-1938 - G U CUCCAG AAACG CCCACAACC 22 17469

HSV-UL54-1939 - CGUCUCCAGAAACGCCCACAACC 23 17470

HSV-UL54-1940 - GCGUCUCCAG AAACG CCCACAACC 24 17471

HSV-UL54-2912 - GAGAUCGACUACACGACC 18 18443

HSV-UL54-2913 - GGAGAUCGACUACACGACC 19 18444

HSV-UL54-964 - CGGAGAUCGACUACACGACC 20 7300

HSV-UL54-2914 - UCGGAGAUCGACUACACGACC 21 18445

HSV-UL54-2915 - GUCGGAGAUCGACUACACGACC 22 18446

HSV-UL54-2916 - UGUCGGAGAUCGACUACACGACC 23 18447

HSV-UL54-2917 - GUGUCGGAGAUCGACUACACGACC 24 18448

HSV-UL54-2918 - CACAU U CG CAG CCA ACCC 18 18449

HSV-UL54-2919 - G CACAU U CG CAG CCAACCC 19 18450

HSV-UL54-944 - CGCACAUUCGCAGCCAACCC 20 7285

HSV-UL54-2920 - CCG CACAU U CG CAGCCAACCC 21 18451

HSV-UL54-2921 - ACCGCACAUUCGCAGCCAACCC 22 18452

HSV-UL54-2922 - UACCGCACAUUCGCAGCCAACCC 23 18453

HSV-UL54-2923 - CUACCGCACAUUCGCAGCCAACCC 24 18454

HSV-UL54-2924 - AAUCUGCCGCUCCGCCCC 18 18455

HSV-UL54-2925 - CAAUCUGCCGCUCCGCCCC 19 18456

HSV-UL54-950 - ACAAUCUGCCGCUCCGCCCC 20 7289

HSV-UL54-2926 - CACAAUCUGCCGCUCCGCCCC 21 18457

HSV-UL54-2927 - CCACAAUCUGCCGCUCCGCCCC 22 18458

HSV-UL54-2928 - ACCACAAUCUGCCGCUCCGCCCC 23 18459

HSV-UL54-2929 - CACCACAAUCUGCCGCUCCGCCCC 24 18460

HSV-UL54-2930 - UCCCGCCGCGAACAGCCC 18 18461

HSV-UL54-2931 - UUCCCGCCGCGAACAGCCC 19 18462

HSV-UL54-932 - UUUCCCGCCGCGAACAGCCC 20 7279

HSV-UL54-2932 - G U U U CCCG CCG CG AACAG CCC 21 18463

HSV-UL54-2933 - CGUUUCCCGCCGCGAACAGCCC 22 18464

HSV-UL54-2934 - CCGUUUCCCGCCGCGAACAGCCC 23 18465 HSV-UL54-2935 - GCCGUUUCCCGCCGCGAACAGCCC 24 18466

HSV-UL54-2936 - GAUGCACUUUUACAUCCC 18 18467

HSV-UL54-2937 - CGAUGCACUUUUACAUCCC 19 18468

HSV-UL54-358 - ACGAUGCACUUUUACAUCCC 20 6781

HSV-UL54-2938 - GACGAUGCACUUUUACAUCCC 21 18469

HSV-UL54-2939 - AGACGAUGCACUUUUACAUCCC 22 18470

HSV-UL54-2940 - GAGACGAUGCACUUUUACAUCCC 23 18471

HSV-UL54-2941 - CGAGACGAUGCACUUUUACAUCCC 24 18472

HSV-UL54-2942 - GGGAUGCCGUUUCCCGCC 18 18473

HSV-UL54-2943 - CGGGAUGCCGUUUCCCGCC 19 18474

HSV-UL54-931 - GCGGGAUGCCGUUUCCCGCC 20 7278

HSV-UL54-2944 - GGCGGGAUGCCGUUUCCCGCC 21 18475

HSV-UL54-2945 - UGGCGGGAUGCCGUUUCCCGCC 22 18476

HSV-UL54-2946 - UUGGCGGGAUGCCGUUUCCCGCC 23 18477

HSV-UL54-2947 - UUUGGCGGGAUGCCGUUUCCCGCC 24 18478

HSV-UL54-2948 - GCGACUGCGUGCUGCGCC 18 18479

HSV-UL54-2949 - CGCGACUGCGUGCUGCGCC 19 18480

HSV-UL54-326 - GCGCGACUGCGUGCUGCGCC 20 6749

HSV-UL54-2950 - UGCGCGACUGCGUGCUGCGCC 21 18481

HSV-UL54-2951 - AUGCGCGACUGCGUGCUGCGCC 22 18482

HSV-UL54-2952 - CAUGCGCGACUGCGUGCUGCGCC 23 18483

HSV-UL54-2953 - CCAUGCGCGACUGCGUGCUGCGCC 24 18484

HSV-UL54-2954 - CUCCGGGGGCCCGCGGCC 18 18485

HSV-UL54-2955 - UCUCCGGGGGCCCGCGGCC 19 18486

HSV-UL54-910 - GUCUCCGGGGGCCCGCGGCC 20 7264

HSV-UL54-2956 - CGUCUCCGGGGGCCCGCGGCC 21 18487

HSV-UL54-2957 - ACGUCUCCGGGGGCCCGCGGCC 22 18488

HSV-UL54-2958 - GACGUCUCCGGGGGCCCGCGGCC 23 18489

HSV-UL54-2959 - CGACGUCUCCGGGGGCCCGCGGCC 24 18490

HSV-UL54-2960 - CGAUGCACUUUUACAUCC 18 18491

HSV-UL54-2961 - ACGAUGCACUUUUACAUCC 19 18492

HSV-UL54-357 - GACGAUGCACUUUUACAUCC 20 6780

HSV-UL54-2962 - AGACGAUGCACUUUUACAUCC 21 18493

HSV-UL54-2963 - GAGACGAUGCACUUUUACAUCC 22 18494

HSV-UL54-2964 - CGAGACGAUGCACUUUUACAUCC 23 18495

HSV-UL54-2965 - GCGAGACGAUGCACUUUUACAUCC 24 18496

HSV-UL54-2966 - GAGCAGCUCGACGUCUCC 18 18497

HSV-UL54-2967 - GGAGCAGCUCGACGUCUCC 19 18498

HSV-UL54-286 - GGGAGCAGCUCGACGUCUCC 20 6709

HSV-UL54-2968 - GGGGAGCAGCUCGACGUCUCC 21 18499

HSV-UL54-2969 - AGGGGAGCAGCUCGACGUCUCC 22 18500

HSV-UL54-2970 - GAGGGGAGCAGCUCGACGUCUCC 23 18501

HSV-UL54-2971 - GGAGGGGAGCAGCUCGACGUCUCC 24 18502

HSV-UL54-2972 - ACGGCCGCGCCCCGGUCC 18 18503

HSV-UL54-2973 - GACGGCCGCGCCCCGGUCC 19 18504

HSV-UL54-296 - GGACGGCCGCGCCCCGGUCC 20 6719 HSV-UL54-2974 - CGGACGGCCGCGCCCCGGUCC 21 18505

HSV-UL54-2975 - GCGGACGGCCGCGCCCCGGUCC 22 18506

HSV-UL54-2976 - CGCGGACGGCCGCGCCCCGGUCC 23 18507

HSV-UL54-2977 - ACGCGGACGGCCGCGCCCCGGUCC 24 18508

HSV-UL54-2978 - GAGACCCGUCGGGUUUCC 18 18509

HSV-UL54-2979 - CGAGACCCGUCGGGUUUCC 19 18510

HSV-UL54-319 - CCGAGACCCGUCGGGUUUCC 20 6742

HSV-UL54-2980 - GCCGAGACCCGUCGGGUUUCC 21 18511

HSV-UL54-2981 - CGCCGAGACCCGUCGGGUUUCC 22 18512

HSV-UL54-2982 - ACGCCGAGACCCGUCGGGUUUCC 23 18513

HSV-UL54-2983 - GACGCCGAGACCCGUCGGGUUUCC 24 18514

HSV-UL54-2984 - CGUGCUGGCCACCCAAGC 18 18515

HSV-UL54-2985 - CCGUGCUGGCCACCCAAGC 19 18516

HSV-UL54-312 - CCCGUGCUGGCCACCCAAGC 20 6735

HSV-UL54-2986 - UCCCGUGCUGGCCACCCAAGC 21 18517

HSV-UL54-2987 - CUCCCGUGCUGGCCACCCAAGC 22 18518

HSV-UL54-2988 - GCUCCCGUGCUGGCCACCCAAGC 23 18519

HSV-UL54-2989 - GGCUCCCGUGCUGGCCACCCAAGC 24 18520

HSV-UL54-2990 - AUACUGGACACGCACCGC 18 18521

HSV-UL54-2991 - AAUACUGGACACGCACCGC 19 18522

HSV-UL54-976 - AAAUACUGGACACGCACCGC 20 7307

HSV-UL54-2992 - GAAAUACUGGACACGCACCGC 21 18523

HSV-UL54-2993 - UGAAAUACUGGACACGCACCGC 22 18524

HSV-UL54-2994 - UUG AAAUACUGGACACGCACCGC 23 18525

HSV-UL54-2995 - AUU GAAAUACUGGACACGCACCGC 24 18526

HSV-UL54-2996 - CGCGACUGCGUGCUGCGC 18 18527

HSV-UL54-2997 - GCGCGACUGCGUGCUGCGC 19 18528

HSV-UL54-945 - UGCGCGACUGCGUGCUGCGC 20 7286

HSV-UL54-2998 - AUGCGCGACUGCGUGCUGCGC 21 18529

HSV-UL54-2999 - CAUGCGCGACUGCGUGCUGCGC 22 18530

HSV-UL54-3000 - CCAUGCGCGACUGCGUGCUGCGC 23 18531

HSV-UL54-3001 - GCCAUGCGCGACUGCGUGCUGCGC 24 18532

HSV-UL54-3002 - GCAGUGCUACCUGAAGGC 18 18533

HSV-UL54-3003 - UGCAGUGCUACCUGAAGGC 19 18534

HSV-UL54-956 - CUGCAGUGCUACCUGAAGGC 20 7293

HSV-UL54-3004 - UCUGCAGUGCUACCUGAAGGC 21 18535

HSV-UL54-3005 - UUCUGCAGUGCUACCUGAAGGC 22 18536

HSV-UL54-3006 - UUUCUGCAGUGCUACCUGAAGGC 23 18537

HSV-UL54-3007 - CUUUCUGCAGUGCUACCUGAAGGC 24 18538

HSV-UL54-3008 - ACCCUGGUCGCUCACGGC 18 18539

HSV-UL54-3009 - AACCCUGGUCGCUCACGGC 19 18540

HSV-UL54-943 - AAACCCUGGUCGCUCACGGC 20 7284

HSV-UL54-3010 - GAAACCCUGGUCGCUCACGGC 21 18541

HSV-UL54-3011 - GG AAACCCU G G U CG CU CACG GC 22 18542

HSV-UL54-3012 - GGGAAACCCUGGUCGCUCACGGC 23 18543

HSV-UL54-3013 - UGGGAAACCCUGGUCGCUCACGGC 24 18544 HSV-UL54-3014 - CCCACGGCGAGGCGCGGC 18 18545

HSV-UL54-3015 - ACCCACGGCGAGGCGCGGC 19 18546

HSV-UL54-908 - CACCCACGGCGAGGCGCGGC 20 7263

HSV-UL54-3016 - CCACCCACGGCGAGGCGCGGC 21 18547

HSV-UL54-3017 - GCCACCCACGGCGAGGCGCGGC 22 18548

HSV-UL54-3018 - CGCCACCCACGGCGAGGCGCGGC 23 18549

HSV-UL54-3019 - GCGCCACCCACGGCGAGGCGCGGC 24 18550

HSV-UL54-3020 - UCGCCACCCCGCGUCGGC 18 18551

HSV-UL54-3021 - GUCGCCACCCCGCGUCGGC 19 18552

HSV-UL54-911 - GGUCGCCACCCCGCGUCGGC 20 7265

HSV-UL54-3022 - GGGUCGCCACCCCGCGUCGGC 21 18553

HSV-UL54-3023 - GGGGUCGCCACCCCGCGUCGGC 22 18554

HSV-UL54-3024 - GGGGGUCGCCACCCCGCGUCGGC 23 18555

HSV-UL54-3025 - AGGGGGUCGCCACCCCGCGUCGGC 24 18556

HSV-UL54-3026 - GAACGGCGGCCGCCGUGC 18 18557

HSV-UL54-3027 - GGAACGGCGGCCGCCGUGC 19 18558

HSV-UL54-336 - CGGAACGGCGGCCGCCGUGC 20 6759

HSV-UL54-3028 - UCGGAACGGCGGCCGCCGUGC 21 18559

HSV-UL54-3029 - AUCGGAACGGCGGCCGCCGUGC 22 18560

HSV-UL54-3030 - CAUCGGAACGGCGGCCGCCGUGC 23 18561

HSV-UL54-3031 - UCAUCGGAACGGCGGCCGCCGUGC 24 18562

HSV-UL54-3032 - ACGAUGCACUUUUACAUC 18 18563

HSV-UL54-3033 - GACGAUGCACUUUUACAUC 19 18564

HSV-UL54-970 - AGACGAUGCACUUUUACAUC 20 7303

HSV-UL54-3034 - GAGACGAUGCACUUUUACAUC 21 18565

HSV-UL54-3035 - CGAGACGAUGCACUUUUACAUC 22 18566

HSV-UL54-3036 - GCGAGACGAUGCACUUUUACAUC 23 18567

HSV-UL54-3037 - GGCGAGACGAUGCACUUUUACAUC 24 18568

HSV-UL54-3038 - GGAGCAGCUCGACGUCUC 18 18569

HSV-UL54-3039 - GGGAGCAGCUCGACGUCUC 19 18570

HSV-UL54-285 - GGGGAGCAGCUCGACGUCUC 20 6708

HSV-UL54-3040 - AGGGGAGCAGCUCGACGUCUC 21 18571

HSV-UL54-3041 - GAGGGGAGCAGCUCGACGUCUC 22 18572

HSV-UL54-3042 - GG AGGGGAGCAGCUCGACGUCUC 23 18573

HSV-UL54-3043 - CGGAGGGGAGCAGCUCGACGUCUC 24 18574

HSV-UL54-2031 - ACCCCGCCGGCGCGUCUC 18 17562

HSV-UL54-2032 - AACCCCGCCGGCGCGUCUC 19 17563

HSV-UL54-2033 - AAACCCCGCCGGCGCGUCUC 20 17564

HSV-UL54-2034 - AAAACCCCGCCGGCGCGUCUC 21 17565

HSV-UL54-2035 - CAAAACCCCGCCGGCGCGUCUC 22 17566

HSV-UL54-2036 - CCAAAACCCCGCCGGCGCGUCUC 23 17567

HSV-UL54-2037 - ACCAAAACCCCGCCGGCGCGUCUC 24 17568

HSV-UL54-3044 - GACGGCCGCGCCCCGGUC 18 18575

HSV-UL54-3045 - GGACGGCCGCGCCCCGGUC 19 18576

HSV-UL54-921 - CGGACGGCCGCGCCCCGGUC 20 7270

HSV-UL54-3046 - GCGGACGGCCGCGCCCCGGUC 21 18577 HSV-UL54-3047 - CGCGGACGGCCGCGCCCCGGUC 22 18578

HSV-UL54-3048 - ACGCGGACGGCCGCGCCCCGGUC 23 18579

HSV-UL54-3049 - CACGCGGACGGCCGCGCCCCGGUC 24 18580

HSV-UL54-3050 - CGAGACCCGUCGGGUUUC 18 18581

HSV-UL54-3051 - CCGAGACCCGUCGGGUUUC 19 18582

HSV-UL54-940 - GCCGAGACCCGUCGGGUUUC 20 7283

HSV-UL54-3052 - CGCCGAGACCCGUCGGGUUUC 21 18583

HSV-UL54-3053 - ACGCCGAGACCCGUCGGGUUUC 22 18584

HSV-UL54-3054 - GACGCCGAGACCCGUCGGGUUUC 23 18585

HSV-UL54-3055 - UGACGCCGAGACCCGUCGGGUUUC 24 18586

HSV-UL54-3056 - CCGUGCUGGCCACCCAAG 18 18587

HSV-UL54-3057 - CCCGUGCUGGCCACCCAAG 19 18588

HSV-UL54-311 - UCCCGUGCUGGCCACCCAAG 20 6734

HSV-UL54-3058 - CUCCCGUGCUGGCCACCCAAG 21 18589

HSV-UL54-3059 - GCUCCCGUGCUGGCCACCCAAG 22 18590

HSV-UL54-3060 - GGCUCCCGUGCUGGCCACCCAAG 23 18591

HSV-UL54-3061 - GGGCUCCCGUGCUGGCCACCCAAG 24 18592

HSV-UL54-3062 - GCGAGGCGCGGCGCGGAG 18 18593

HSV-UL54-3063 - GGCGAGGCGCGGCGCGGAG 19 18594

HSV-UL54-284 - CGGCGAGGCGCGGCGCGGAG 20 6707

HSV-UL54-3064 - ACGGCGAGGCGCGGCGCGGAG 21 18595

HSV-UL54-3065 - CACGGCGAGGCGCGGCGCGGAG 22 18596

HSV-UL54-3066 - CCACGGCGAGGCGCGGCGCGGAG 23 18597

HSV-UL54-3067 - CCCACGGCGAGGCGCGGCGCGGAG 24 18598

HSV-UL54-3068 - GCCCCGGCGCCACCCACG 18 18599

HSV-UL54-3069 - GGCCCCGGCGCCACCCACG 19 18600

HSV-UL54-913 - CGGCCCCGGCGCCACCCACG 20 7267

HSV-UL54-3070 - ACGGCCCCGGCGCCACCCACG 21 18601

HSV-UL54-3071 - GACGGCCCCGGCGCCACCCACG 22 18602

HSV-UL54-3072 - GGACGGCCCCGGCGCCACCCACG 23 18603

HSV-UL54-3073 - CGGACGGCCCCGGCGCCACCCACG 24 18604

HSV-UL54-3074 - AAGCGGGGGGGUUUGACG 18 18605

HSV-UL54-3075 - CAAGCGGGGGGGUUUGACG 19 18606

HSV-UL54-938 - CCAAGCGGGGGGGUUUGACG 20 7281

HSV-UL54-3076 - CCCAAGCGGGGGGGUUUGACG 21 18607

HSV-UL54-3077 - ACCCAAGCGGGGGGGUUUGACG 22 18608

HSV-UL54-3078 - CACCCAAGCGGGGGGGU UUGACG 23 18609

HSV-UL54-3079 - CCACCCAAGCGGGGGGGUUUGACG 24 18610

HSV-UL54-3080 - AG AU CGACU ACACGACCG 18 18611

HSV-UL54-3081 - GAGAUCGACUACACGACCG 19 18612

HSV-UL54-349 - GGAGAUCGACU ACACGACCG 20 6772

HSV-UL54-3082 - CGGAGAUCGACU ACACGACCG 21 18613

HSV-UL54-3083 - UCGGAGAUCGACU ACACGACCG 22 18614

HSV-UL54-3084 - GUCGGAGAUCGACUACACGACCG 23 18615

HSV-UL54-3085 - UGUCGGAGAUCGACUACACGACCG 24 18616

HSV-UL54-3086 - CCGUGGGGGUUGGGGCCG 18 18617 HSV-UL54-3087 - ACCGUGGGGGUUGGGGCCG 19 18618

HSV-UL54-969 - GACCGUGGGGGUUGGGGCCG 20 7302

HSV-UL54-3088 - CGACCGUGGGGGU UGGGGCCG 21 18619

HSV-UL54-3089 - ACGACCGUGGGGGUUGGGGCCG 22 18620

HSV-UL54-3090 - CACGACCGUGGGGGUUGGGGCCG 23 18621

HSV-UL54-3091 - ACACGACCGUGGGGGUUGGGGCCG 24 18622

HSV-UL54-3092 - GUGCUGGCCACCCAAGCG 18 18623

HSV-UL54-3093 - CGUGCUGGCCACCCAAGCG 19 18624

HSV-UL54-313 - CCGUGCUGGCCACCCAAGCG 20 6736

HSV-UL54-3094 - CCCGUGCUGGCCACCCAAGCG 21 18625

HSV-UL54-3095 - UCCCGUGCUGGCCACCCAAGCG 22 18626

HSV-UL54-3096 - CUCCCGUGCUGG CCACCCAAG CG 23 18627

HSV-UL54-3097 - GCUCCCGUGCUGGCCACCCAAGCG 24 18628

HSV-UL54-3098 - CCCGCCUCGCCAACCGCG 18 18629

HSV-UL54-3099 - GCCCGCCUCGCCAACCGCG 19 18630

HSV-UL54-961 - GGCCCGCCUCGCCAACCGCG 20 7298

HSV-UL54-3100 - UGGCCCGCCUCGCCAACCGCG 21 18631

HSV-UL54-3101 - CUGGCCCGCCUCGCCAACCGCG 22 18632

HSV-UL54-3102 - CCUGGCCCGCCUCGCCAACCGCG 23 18633

HSV-UL54-3103 - UCCUGGCCCGCCUCGCCAACCGCG 24 18634

HSV-UL54-3104 - ACGGCGAGGCGCGGCGCG 18 18635

HSV-UL54-3105 - CACGGCGAGGCGCGGCGCG 19 18636

HSV-UL54-907 - CCACGGCGAGGCGCGGCGCG 20 7262

HSV-UL54-3106 - CCCACGGCGAGGCGCGGCGCG 21 18637

HSV-UL54-3107 - ACCCACGGCGAGGCGCGGCGCG 22 18638

HSV-UL54-3108 - CACCCACGGCGAGGCGCGGCGCG 23 18639

HSV-UL54-3109 - CCACCCACGGCGAGGCGCGGCGCG 24 18640

HSV-UL54-3110 - CCACGGCGAGGCGCGGCG 18 18641

HSV-UL54-3111 - CCCACGGCGAGGCGCGGCG 19 18642

HSV-UL54-281 - ACCCACGGCGAGGCGCGGCG 20 6704

HSV-UL54-3112 - CACCCACGGCGAGGCGCGGCG 21 18643

HSV-UL54-3113 - CCACCCACGGCGAGGCGCGGCG 22 18644

HSV-UL54-3114 - GCCACCCACGGCGAGGCGCGGCG 23 18645

HSV-UL54-3115 - CGCCACCCACGGCGAGGCGCGGCG 24 18646

HSV-UL54-3116 - AGGCCCUGGCGUCCGCGG 18 18647

HSV-UL54-3117 - GAGGCCCUGGCGUCCGCGG 19 18648

HSV-UL54-671 - CGAGGCCCUGGCGUCCGCGG 20 7089

HSV-UL54-3118 - UCGAGGCCCUGGCGUCCGCGG 21 18649

HSV-UL54-3119 - AUCGAGGCCCUGGCGUCCGCGG 22 18650

HSV-UL54-3120 - CAUCGAGGCCCUGGCGUCCGCGG 23 18651

HSV-UL54-3121 - UCAUCGAGGCCCUGGCGUCCGCGG 24 18652

HSV-UL54-3122 - CGGCGAGGCGCGGCGCGG 18 18653

HSV-UL54-3123 - ACGGCGAGGCGCGGCGCGG 19 18654

HSV-UL54-282 - CACGGCGAGGCGCGGCGCGG 20 6705

HSV-UL54-3124 - CCACGGCGAGGCGCGGCGCGG 21 18655

HSV-UL54-3125 - CCCACGGCGAGGCGCGGCGCGG 22 18656 HSV-UL54-3126 - ACCCACGGCGAGGCGCGGCGCGG 23 18657

HSV-UL54-3127 - CACCCACGGCGAGGCGCGGCGCGG 24 18658

HSV-UL54-3128 - CACCCCGCGUCGGCGCGG 18 18659

HSV-UL54-3129 - CCACCCCGCGUCGGCGCGG 19 18660

HSV-UL54-912 - GCCACCCCGCGUCGGCGCGG 20 7266

HSV-UL54-3130 - CGCCACCCCGCGUCGGCGCGG 21 18661

HSV-UL54-3131 - UCGCCACCCCGCGUCGGCGCGG 22 18662

HSV-UL54-3132 - GUCGCCACCCCGCGUCGGCGCGG 23 18663

HSV-UL54-3133 - GGUCGCCACCCCGCGUCGGCGCGG 24 18664

HSV-UL54-3134 - UAUCCGAGCGCGCGGCGG 18 18665

HSV-UL54-3135 - AUAUCCGAGCGCGCGGCGG 19 18666

HSV-UL54-926 - CAUAUCCGAGCGCGCGGCGG 20 7274

HSV-UL54-3136 - CCAUAUCCGAGCGCGCGGCGG 21 18667

HSV-UL54-3137 - UCCAUAUCCGAGCGCGCGGCGG 22 18668

HSV-UL54-3138 - AUCCAUAUCCGAGCGCGCGGCGG 23 18669

HSV-UL54-3139 - GAUCCAUAUCCGAGCGCGCGGCGG 24 18670

HSV-UL54-3140 - GCCCGAGGCCUGUGCGGG 18 18671

HSV-UL54-3141 - GGCCCGAGGCCUGUGCGGG 19 18672

HSV-UL54-958 - AGGCCCGAGGCCUGUGCGGG 20 7295

HSV-UL54-3142 - AAGGCCCGAGGCCUGUGCGGG 21 18673

HSV-UL54-3143 - GAAGGCCCGAGGCCUGUGCGGG 22 18674

HSV-UL54-3144 - UG AAGGCCCGAGGCCUGUGCGGG 23 18675

HSV-UL54-3145 - CUGAAGGCCCGAGGCCUGUGCGGG 24 18676

HSV-UL54-3146 - ACUACACGACCGUGGGGG 18 18677

HSV-UL54-3147 - GACUACACGACCGUGGGGG 19 18678

HSV-UL54-967 - CGACUACACGACCGUGGGGG 20 7301

HSV-UL54-3148 - UCG ACUACACGACCGUGGGGG 21 18679

HSV-UL54-3149 - AUCGACUACACGACCGUGGGGG 22 18680

HSV-UL54-3150 - GAUCGACUACACGACCGUGGGGG 23 18681

HSV-UL54-3151 - AGAUCGACUACACGACCGUGGGGG 24 18682

HSV-UL54-3152 - UGCUCGCGGCGACGCCUG 18 18683

HSV-UL54-3153 - GUGCUCGCGGCGACGCCUG 19 18684

HSV-UL54-959 - UGUGCUCGCGGCGACGCCUG 20 7296

HSV-UL54-3154 - CUGUGCUCGCGGCGACGCCUG 21 18685

HSV-UL54-3155 - CCUGUGCUCGCGGCGACGCCUG 22 18686

HSV-UL54-3156 - ACCUGUGCUCGCGGCGACGCCUG 23 18687

HSV-UL54-3157 - GACCUGUGCUCGCGGCGACGCCUG 24 18688

HSV-UL54-3158 - GGAACGGCGGCCGCCGUG 18 18689

HSV-UL54-3159 - CGGAACGGCGGCCGCCGUG 19 18690

HSV-UL54-953 - UCGGAACGGCGGCCGCCGUG 20 7291

HSV-UL54-3160 - AUCGGAACGGCGGCCGCCGUG 21 18691

HSV-UL54-3161 - CAUCGGAACGGCGGCCGCCGUG 22 18692

HSV-UL54-3162 - UCAUCGGAACGGCGGCCGCCGUG 23 18693

HSV-UL54-3163 - AUCAUCGGAACGGCGGCCGCCGUG 24 18694

HSV-UL54-3164 - CGCGUCGAGCGCGGCGUG 18 18695

HSV-UL54-3165 - CCGCGUCGAGCGCGGCGUG 19 18696 HSV-UL54-962 - ACCGCGUCGAGCGCGGCGUG 20 7299

HSV-UL54-3166 - AACCGCGUCGAGCGCGGCGUG 21 18697

HSV-UL54-3167 - CAACCGCGUCGAGCGCGGCGUG 22 18698

HSV-UL54-3168 - CCAACCGCGUCGAGCGCGGCGUG 23 18699

HSV-UL54-3169 - GCCAACCGCGUCGAGCGCGGCGUG 24 18700

HSV-UL54-3170 - U CAU G CAAG ACCCCU U U G 18 18701

HSV-UL54-3171 - GUCAUGCAAGACCCCUUUG 19 18702

HSV-UL54-929 - GGUCAUGCAAGACCCCUUUG 20 7277

HSV-UL54-3172 - UGGUCAUGCAAGACCCCUUUG 21 18703

HSV-UL54-3173 - CUGGUCAUGCAAGACCCCUUUG 22 18704

HSV-UL54-3174 - CCUGGUCAUGCAAGACCCCUUUG 23 18705

HSV-UL54-3175 - CCCUGG UCAUGCAAGACCCCU U UG 24 18706

HSV-UL54-3176 - CCCCCAGGACCCUAUCAU 18 18707

HSV-UL54-3177 - GCCCCCAGGACCCUAUCAU 19 18708

HSV-UL54-333 - CGCCCCCAGGACCCUAUCAU 20 6756

HSV-UL54-3178 - CCGCCCCCAGGACCCUAUCAU 21 18709

HSV-UL54-3179 - UCCGCCCCCAGGACCCUAUCAU 22 18710

HSV-UL54-3180 - CUCCGCCCCCAGGACCCUAUCAU 23 18711

HSV-UL54-3181 - GCUCCGCCCCCAGGACCCUAUCAU 24 18712

HSV-UL54-3182 - CGGUUCUGCGAUCCAUAU 18 18713

HSV-UL54-3183 - GCGGUUCUGCGAUCCAUAU 19 18714

HSV-UL54-925 - GGCGGUUCUGCGAUCCAUAU 20 7273

HSV-UL54-3184 - GGGCGGUUCUGCGAUCCAUAU 21 18715

HSV-UL54-3185 - CGGGCGGUUCUGCGAUCCAUAU 22 18716

HSV-UL54-3186 - UCGGGCGGUUCUGCGAUCCAUAU 23 18717

HSV-UL54-3187 - UUCGGGCGGUUCUGCGAUCCAUAU 24 18718

HSV-UL54-3188 - AGACCCGUCGGGUUUCCU 18 18719

HSV-UL54-3189 - GAGACCCGUCGGGUUUCCU 19 18720

HSV-UL54-320 - CGAGACCCGUCGGGUU UCCU 20 6743

HSV-UL54-3190 - CCGAGACCCG UCGGGU U UCCU 21 18721

HSV-UL54-3191 - GCCGAGACCCGUCGGGUUUCCU 22 18722

HSV-UL54-3192 - CGCCGAGACCCGUCGGGUUUCCU 23 18723

HSV-UL54-3193 - ACGCCGAGACCCGUCGGGUUUCCU 24 18724

HSV-UL54-3194 - AGCGCAUCAGCGAAAGCU 18 18725

HSV-UL54-3195 - GAGCGCAUCAGCGAAAGCU 19 18726

HSV-UL54-928 - CG AGCGCAUCAGCGAAAGCU 20 7276

HSV-UL54-3196 - UCGAGCGCAUCAGCGAAAGCU 21 18727

HSV-UL54-3197 - GUCGAGCGCAUCAGCGAAAGCU 22 18728

HSV-UL54-3198 - GG UCGAGCGCAUCAGCGAAAGCU 23 18729

HSV-UL54-3199 - CGGUCGAGCGCAUCAGCGAAAGCU 24 18730

HSV-UL54-3200 - GGGAGCAGCUCGACGUCU 18 18731

HSV-UL54-3201 - GGGGAGCAGCUCGACGUCU 19 18732

HSV-UL54-917 - AGGGGAGCAGCUCGACGUCU 20 7268

HSV-UL54-3202 - GAGGGGAGCAGCUCGACGUCU 21 18733

HSV-UL54-3203 - GGAGGGGAGCAGCUCGACGUCU 22 18734

HSV-UL54-3204 - CGG AGGGGAGCAGCUCGACGUCU 23 18735 HSV-UL54-3205 - GCGGAGGGGAGCAGCUCGACGUCU 24 18736

HSV-UL54-3206 - CACCAUCGACCCCGCCGU 18 18737

HSV-UL54-3207 - ACACCAUCGACCCCGCCGU 19 18738

HSV-UL54-924 - GACACCAUCGACCCCGCCGU 20 7272

HSV-UL54-3208 - CGACACCAUCGACCCCGCCGU 21 18739

HSV-UL54-3209 - CCGACACCAUCGACCCCGCCGU 22 18740

HSV-UL54-3210 - GCCGACACCAUCGACCCCGCCGU 23 18741

HSV-UL54-3211 - U GCCG ACACCAU CG ACCCCG CCG U 24 18742

HSV-UL54-3212 - CUACACGACCGUGGGGGU 18 18743

HSV-UL54-3213 - ACUACACGACCGUGGGGGU 19 18744

HSV-UL54-353 - GACUACACGACCGUGGGGGU 20 6776

HSV-UL54-3214 - CG ACUACACGACCGUGGGGGU 21 18745

HSV-UL54-3215 - UCGACUACACGACCGUGGGGGU 22 18746

HSV-UL54-3216 - AUCGACUACACGACCGUGGGGGU 23 18747

HSV-UL54-3217 - GAUCGACUACACGACCGUGGGGGU 24 18748

HSV-UL54-3218 - UGAAGGCCCGAGGCCUGU 18 18749

HSV-UL54-3219 - CUGAAGGCCCGAGGCCUGU 19 18750

HSV-UL54-957 - CCUGAAGGCCCGAGGCCUGU 20 7294

HSV-UL54-3220 - ACCUGAAGGCCCGAGGCCUGU 21 18751

HSV-UL54-3221 - UACCUGAAGGCCCGAGGCCUGU 22 18752

HSV-UL54-3222 - CUACCUGAAGGCCCGAGGCCUGU 23 18753

HSV-UL54-3223 - GCUACCUGAAGGCCCGAGGCCUGU 24 18754

HSV-UL54-3224 - AGUGUUCCAGUCGCGUGU 18 18755

HSV-UL54-3225 - GAGUGUUCCAGUCGCGUGU 19 18756

HSV-UL54-978 - GGAGUGUUCCAGUCGCGUGU 20 7308

HSV-UL54-3226 - AGGAGUGUUCCAGUCGCGUGU 21 18757

HSV-UL54-3227 - CAGGAGUGUUCCAGUCGCGUGU 22 18758

HSV-UL54-3228 - CCAGGAGUGUUCCAGUCGCGUGU 23 18759

HSV-UL54-3229 - GCCAGGAGUGUUCCAGUCGCGUGU 24 18760

HSV-UL54-3230 - GCGUCGAGCGCGGCGUGU 18 18761

HSV-UL54-3231 - CGCGUCGAGCGCGGCGUGU 19 18762

HSV-UL54-348 - CCGCGUCGAGCGCGGCGUGU 20 6771

HSV-UL54-3232 - ACCGCGUCGAGCGCGGCGUGU 21 18763

HSV-UL54-3233 - AACCGCGUCGAGCGCGGCGUGU 22 18764

HSV-UL54-3234 - CAACCGCGUCGAGCGCGGCGUGU 23 18765

HSV-UL54-3235 - CCAACCGCGUCGAGCGCGGCGUGU 24 18766

Table 16A provides exemplary targeting domains for knocking out the UL54 gene selected according to the first tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon), have a high level of orthogonality and start with a 5'G. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase).

Table 16A

Table 16B provides exemplary targeting domains for knocking out the UL54 gene selected according to the second tier parameters. The targeting domains bind within the first 500 bp of the coding sequence (e.g., within 500 bp downstream from the start codon) and have a high level of orthogonality. It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 16B

Table 16C provides exemplary targeting domains for knocking out the UL54 gene selected according to the fifth tier parameters. The targeting domains fall in the coding sequence of the gene, downstream of the first 500bp of coding sequence (e.g., anywhere from +500 (relative to the start codon) to the stop codon of the gene). It is contemplated herein that in an embodiment the targeting domain hybridizes to the target domain through

complementary base pairing. Any of the targeting domains in the table can be used with a N. meningitidis Cas9 molecule that generates a double stranded break (Cas9 nuclease) or a single- stranded break (Cas9 nickase). Table 16C

III. Cas9 Molecules

Cas9 molecules of a variety of species can be used in the methods and compositions described herein. While the S. pyogenes, S. aureus and S. thermophilus Cas9 molecules are the subject of much of the disclosure herein, Cas9 molecules of, derived from, or based on the Cas9 proteins of other species listed herein can be used as well. In other words, while the much of the description herein uses S. pyogenes and S. thermophilus Cas9 molecules, Cas9 molecules from the other species can replace them, e.g., Staphylococcus aureus and Neisseria meningitidis Cas9 molecules. Additional Cas9 species include: Acidovorax avenae, Actinobacillus pleuropneumoniae, Actinobacillus succinogenes, Actinobacillus suis, Actinomyces sp., cycliphilus denitrificans, Aminomonas paucivorans, Bacillus cereus, Bacillus smithii, Bacillus thuringiensis, Bacteroides sp., Blastopirellula marina,

Bradyrhizobium sp., Brevibacillus laterosporus, Campylobacter coli, Campylobacter jejuni, Campylobacter lari, Candidatus Puniceispirillum, Clostridium cellulolyticum, Clostridium perfringens, Corynebacterium accolens, Corynebacterium diphtheria, Corynebacterium matruchotii, Dinoroseobacter shibae, Eubacterium dolichum, gamma proteobacterium, Gluconacetobacter diazotrophicus, Haemophilus parainfluenzae, Haemophilus sputorum, Helicobacter canadensis, Helicobacter cinaedi, Helicobacter mustelae, Ilyobacter polytropus, Kingella kingae, Lactobacillus crispatus, Listeria ivanovii, Listeria

monocytogenes, Listeriaceae bacterium, Methylocystis sp., Methylosinus trichosporium, Mobiluncus mulieris, Neisseria bacilliformis, Neisseria cinerea, Neisseria flavescens, Neisseria lactamica, Neisseria meningitidis, Neisseria sp., Neisseria wadsworthii,

Nitrosomonas sp., Parvibaculum lavamentivorans, Pasteurella multocida, Phascolarctobacterium succinatutens, Ralstonia syzygii, Rhodopseudomonas palustris, Rhodovulum sp., Simonsiella muelleri, Sphingomonas sp., Sporolactobacillus vineae, Staphylococcus lugdunensis, Streptococcus sp., Subdoligranulum sp., Tistrella mobilis, Treponema sp., or Verminephrobacter eiseniae.

A Cas9 molecule, or Cas9 polypeptide, as that term is used herein, refers to a molecule or polypeptide that can interact with a guide RNA (gRNA) molecule and, in concert with the gRNA molecule, home or localizes to a site which comprises a target domain and PAM sequence. Cas9 molecule and Cas9 polypeptide, as those terms are used herein, refer to naturally occurring Cas9 molecules and to engineered, altered, or modified Cas9 molecules or Cas9 polypeptides that differ, e.g., by at least one amino acid residue, from a reference sequence, e.g., the most similar naturally occurring Cas9 molecule or a sequence of Table 17 or 18.

Cas9 Domains

Crystal structures have been determined for two different naturally occurring bacterial

Cas9 molecules (Jinek et al., Science, 343(6176): 1247997, 2014) and for 5". pyogenes Cas9 with a guide RNA (e.g., a synthetic fusion of crRNA and tracrRNA) (Nishimasu et al., Cell, 156:935-949, 2014; and Anders et al., Nature, 2014, doi: 10.1038/naturel3579).

A naturally occurring Cas9 molecule comprises two lobes: a recognition (REC) lobe and a nuclease (NUC) lobe; each of which further comprises domains described herein. Figs. 9A-9B provide a schematic of the organization of important Cas9 domains in the primary structure. The domain nomenclature and the numbering of the amino acid residues encompassed by each domain used throughout this disclosure is as described in Nishimasu et al. The numbering of the amino acid residues is with reference to Cas9 from S. pyogenes.

The REC lobe comprises the arginine-rich bridge helix (BH), the REC1 domain, and the REC2 domain. The REC lobe does not share structural similarity with other known proteins, indicating that it is a Cas9-specific functional domain. The BH domain is a long a helix and arginine rich region and comprises amino acids 60-93 of the sequence of S.

pyogenes Cas9. The REC1 domain is important for recognition of the repeat: anti-repeat duplex, e.g., of a gRNA or a tracrRNA, and is therefore critical for Cas9 activity by recognizing the target sequence. The REC1 domain comprises two REC1 motifs at amino acids 94 to 179 and 308 to 717 of the sequence of S. pyogenes Cas9. These two REC1 domains, though separated by the REC2 domain in the linear primary structure, assemble in the tertiary structure to form the REC1 domain. The REC2 domain, or parts thereof, may also play a role in the recognition of the repeat: anti-repeat duplex. The REC2 domain comprises amino acids 180-307 of the sequence of S. pyogenes Cas9.

The NUC lobe comprises the RuvC domain (also referred to herein as RuvC-like domain), the HNH domain (also referred to herein as HNH-like domain), and the PAM- interacting (PI) domain. The RuvC domain shares structural similarity to retroviral integrase superfamily members and cleaves a single strand, e.g., the non-complementary strand of the target nucleic acid molecule. The RuvC domain is assembled from the three split RuvC motifs (RuvC I, RuvCII, and RuvCIII, which are often commonly referred to in the art as RuvCI domain, or N-terminal RuvC domain, RuvCII domain, and RuvCIII domain) at amino acids 1-59, 718-769, and 909-1098, respectively, of the sequence of S. pyogenes Cas9.

Similar to the REC1 domain, the three RuvC motifs are linearly separated by other domains in the primary structure, however in the tertiary structure, the three RuvC motifs assemble and form the RuvC domain. The HNH domain shares structural similarity with HNH endonucleases, and cleaves a single strand, e.g., the complementary strand of the target nucleic acid molecule. The HNH domain lies between the RuvC II-III motifs and comprises amino acids 775-908 of the sequence of S. pyogenes Cas9. The PI domain interacts with the PAM of the target nucleic acid molecule, and comprises amino acids 1099-1368 of the sequence of S. pyogenes Cas9.

A RuvC-like domain and an HNH-like domain

In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises an HNH-like domain and a RuvC-like domain. In an embodiment, cleavage activity is dependent on a RuvC-like domain and an HNH-like domain. A Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, can comprise one or more of the following domains: a RuvC-like domain and an HNH-like domain. In an embodiment, a Cas9 molecule or Cas9 polypeptide is an eaCas9 molecule or eaCas9 polypeptide and the eaCas9 molecule or eaCas9 polypeptide comprises a RuvC-like domain, e.g., a RuvC-like domain described below, and/or an HNH-like domain, e.g., an HNH-like domain described below.

RuvC-like domains

In an embodiment, a RuvC-like domain cleaves, a single strand, e.g., the non- complementary strand of the target nucleic acid molecule. The Cas9 molecule or Cas9 polypeptide can include more than one RuvC-like domain (e.g., one, two, three or more RuvC-like domains). In an embodiment, a RuvC-like domain is at least 5, 6, 7, 8 amino acids in length but not more than 20, 19, 18, 17, 16 or 15 amino acids in length. In an embodiment, the Cas9 molecule or Cas9 polypeptide comprises an N-terminal RuvC-like domain of about 10 to 20 amino acids, e.g., about 15 amino acids in length.

N-terminal RuvC-like domains

Some naturally occurring Cas9 molecules comprise more than one RuvC-like domain with cleavage being dependent on the N-terminal RuvC-like domain. Accordingly, Cas9 molecules or Cas9 polypeptide can comprise an N-terminal RuvC-like domain. Exemplary N-terminal RuvC-like domains are described below.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an N- terminal RuvC-like domain comprising an amino acid sequence of formula I:

D-X1-G-X2-X3-X4-X5-G-X6-X7-X8-X9 (SEQ ID NO: 8),

wherein,

XI is selected from I, V, M, L and T (e.g., selected from I, V, and L);

X2 is selected from T, I, V, S, N, Y, E and L (e.g., selected from T, V, and I);

X3 is selected from N, S, G, A, D, T, R, M and F (e.g., A or N);

X4 is selected from S, Y, N and F (e.g., S);

X5 is selected from V, I, L, C, T and F (e.g., selected from V, I and L);

X6 is selected from W, F, V, Y, S and L (e.g., W);

X7 is selected from A, S, C, V and G (e.g., selected from A and S);

X8 is selected from V, I, L, A, M and H (e.g., selected from V, I, M and L); and

X9 is selected from any amino acid or is absent (e.g., selected from T, V, I, L, Δ, F, S, A, Y, M and R, or, e.g., selected from T, V, I, L and Δ).

In an embodiment, the N-terminal RuvC-like domain differs from a sequence of SEQ

ID NO:8, by as many as 1 but no more than 2, 3, 4, or 5 residues.

In embodiment, the N-terminal RuvC-like domain is cleavage competent.

In embodiment, the N-terminal RuvC-like domain is cleavage incompetent.

In an embodiment, a eaCas9 molecule or eaCas9 polypeptide comprises an N-terminal RuvC-like domain comprising an amino acid sequence of formula II:

D-X1-G-X2-X3-S-X5-G-X6-X7-X8-X9, (SEQ ID NO: 9),

wherein

XI is selected from I, V, M, L and T (e.g., selected from I, V, and L);

X2 is selected from T, I, V, S, N, Y, E and L (e.g., selected from T, V, and I); X3 is selected from N, S, G, A, D, T, R, M and F (e.g., A or N);

X5 is selected from V, I, L, C, T and F (e.g., selected from V, I and L);

X6 is selected from W, F, V, Y, S and L (e.g., W);

X7 is selected from A, S, C, V and G (e.g., selected from A and S);

X8 is selected from V, I, L, A, M and H (e.g., selected from V, I, M and L); and

X9 is selected from any amino acid or is absent (e.g., selected from T, V, I, L, Δ, F, S, A, Y, M and R or selected from e.g., T, V, I, L and Δ).

In an embodiment, the N-terminal RuvC-like domain differs from a sequence of SEQ ID NO: 9 by as many as 1 but no more than 2, 3, 4, or 5 residues.

In an embodiment, the N-terminal RuvC-like domain comprises an amino acid sequence of formula III:

D-I-G-X2-X3-S-V-G-W-A-X8-X9 (SEQ ID NO: 10),

wherein

X2 is selected from T, I, V, S, N, Y, E and L (e.g., selected from T, V, and I);

X3 is selected from N, S, G, A, D, T, R, M and F (e.g., A or N);

X8 is selected from V, I, L, A, M and H (e.g., selected from V, I, M and L); and

X9 is selected from any amino acid or is absent (e.g., selected from T, V, I, L, Δ, F, S, A, Y, M and R or selected from e.g., T, V, I, L and Δ).

In an embodiment, the N-terminal RuvC-like domain differs from a sequence of SEQ ID NO: 10 by as many as 1 but no more than, 2, 3, 4, or 5 residues.

In an embodiment, the N-terminal RuvC-like domain comprises an amino acid sequence of formula III:

D-I-G-T-N-S-V-G-W-A-V-X (SEQ ID NO: 11),

wherein

X is a non-polar alkyl amino acid or a hydroxyl amino acid, e.g., X is selected from

V, I, L and T (e.g., the eaCas9 molecule can comprise an N-terminal RuvC-like domain shown in Figs. 2A-2G (is depicted as Y)).

In an embodiment, the N-terminal RuvC-like domain differs from a sequence of SEQ ID NO: 11 by as many as 1 but no more than, 2, 3, 4, or 5 residues.

In an embodiment, the N-terminal RuvC-like domain differs from a sequence of an N- terminal RuvC like domain disclosed herein, e.g., in Figs. 3A-3B or Figs. 7A-7B, as many as 1 but no more than 2, 3, 4, or 5 residues. In an embodiment, 1, 2, 3 or all of the highly conserved residues identified in Figs. 3A-3B or Figs. 7A-7B are present. In an embodiment, the N-terminal RuvC-like domain differs from a sequence of an N- terminal RuvC-like domain disclosed herein, e.g., in Figs. 4A-4B or Figs. 7A-7B, as many as 1 but no more than 2, 3, 4, or 5 residues. In an embodiment, 1, 2, or all of the highly conserved residues identified in Figs. 4A-4B or Figs. 7A-7B are present.

Additional RuvC-like domains

In addition to the N-terminal RuvC-like domain, the Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, can comprise one or more additional RuvC-like domains. In an embodiment, the Cas9 molecule or Cas9 polypeptide can comprise two additional RuvC-like domains. Preferably, the additional RuvC-like domain is at least 5 amino acids in length and, e.g., less than 15 amino acids in length, e.g., 5 to 10 amino acids in length, e.g., 8 amino acids in length.

An additional RuvC-like domain can comprise an amino acid sequence:

I-X1-X2-E-X3-A-R-E (SEQ ID NO: 12), wherein

XI is V or H,

X2 is I, L or V (e.g., I or V); and

X3 is M or T.

In an embodiment, the additional RuvC-like domain comprises the amino acid sequence:

I-V-X2-E-M-A-R-E (SEQ ID NO: 13), wherein

X2 is I, L or V (e.g., I or V) (e.g., the eaCas9 molecule or eaCas9 polypeptide can comprise an additional RuvC-like domain shown in Fig. 2A-2G or Figs. 7A-7B (depicted as B)).

An additional RuvC-like domain can comprise an amino acid sequence:

H-H-A-X1-D-A-X2-X3 (SEQ ID NO: 14), wherein

XI is H or L;

X2 is R or V; and

X3 is E or V.

In an embodiment, the additional RuvC-like domain comprises the amino acid sequence : H-H- A-H-D- A- Y-L (SEQ ID NO : 15) .

In an embodiment, the additional RuvC-like domain differs from a sequence of SEQ ID NO: 12, 13, 14 or 15 by as many as 1 but no more than 2, 3, 4, or 5 residues.

In some embodiments, the sequence flanking the N-terminal RuvC-like domain is a sequences of formula V: K-X1'-Y-X2'-X3'-X4'-Z-T-D-X9'-Y, (SEQ ID NO: 16).

wherein

XI ' is selected from K and P,

X2' is selected from V, L, I, and F (e.g., V, I and L);

X3' is selected from G, A and S (e.g., G),

X4' is selected from L, I, V and F (e.g., L);

X9' is selected from D, E, N and Q; and

Z is an N-terminal RuvC-like domain, e.g., as described above.

HNH-like domains

In an embodiment, an HNH-like domain cleaves a single stranded complementary domain, e.g., a complementary strand of a double stranded nucleic acid molecule. In an embodiment, an HNH-like domain is at least 15, 20, 25 amino acids in length but not more than 40, 35 or 30 amino acids in length, e.g., 20 to 35 amino acids in length, e.g., 25 to 30 amino acids in length. Exemplary HNH-like domains are described below.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an HNH- like domain having an amino acid sequence of formula VI:

X1-X2-X3-H-X4-X5-P-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-N-X16-X17- X18-X19-X20-X21-X22-X23-N (SEQ ID NO: 17), wherein

XI is selected from D, E, Q and N (e.g., D and E);

X2 is selected from L, I, R, Q, V, M and K;

X3 is selected from D and E;

X4 is selected from I, V, T, A and L (e.g., A, I and V);

X5 is selected from V, Y, I, L, F and W (e.g., V, I and L);

X6 is selected from Q, H, R, K, Y, I, L, F and W;

X7 is selected from S, A, D, T and K (e.g., S and A);

X8 is selected from F, L, V, K, Y, M, I, R, A, E, D and Q (e.g., F);

X9 is selected from L, R, T, I, V, S, C, Y, K, F and G;

X10 is selected from K, Q, Y, T, F, L, W, M, A, E, G, and S;

XI I is selected from D, S, N, R, L and T (e.g., D);

X12 is selected from D, N and S;

X13 is selected from S, A, T, G and R (e.g., S);

X14 is selected from I, L, F, S, R, Y, Q, W, D, K and H (e.g., I, L and F);

X15 is selected from D, S, I, N, E, A, H, F, L, Q, M, G, Y and V; X16 is selected from K, L, R, M, T and F (e.g., L, R and K);

X17 is selected from V, L, I, A and T;

X18 is selected from L, I, V and A (e.g., L and I);

X19 is selected from T, V, C, E, S and A (e.g., T and V);

X20 is selected from R, F, T, W, E, L, N, C, K, V, S, Q, I, Y, H and A;

X21 is selected from S, P, R, K, N, A, H, Q, G and L;

X22 is selected from D, G, T, N, S, K, A, I, E, L, Q, R and Y; and

X23 is selected from K, V, A, E, Y, I, C, L, S, T, G, K, M, D and F.

In an embodiment, a HNH-like domain differs from a sequence of SEQ ID NO: 17 by at least one but no more than, 2, 3, 4, or 5 residues.

In an embodiment, the HNH-like domain is cleavage competent.

In an embodiment, the HNH-like domain is cleavage incompetent.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an HNH- like domain comprising an amino acid sequence of formula VII:

X1-X2-X3-H-X4-X5-P-X6-S-X8-X9-X10-D-D-S-X14-X15-N-K-V-L-X19-X20- X21-X22-X23-N (SEQ ID NO: 18),

wherein

XI is selected from D and E;

X2 is selected from L, I, R, Q, V, M and K;

X3 is selected from D and E;

X4 is selected from I, V, T, A and L (e.g., A, I and V);

X5 is selected from V, Y, I, L, F and W (e.g., V, I and L);

X6 is selected from Q, H, R, K, Y, I, L, F and W;

X8 is selected from F, L, V, K, Y, M, I, R, A, E, D and Q (e.g., F);

X9 is selected from L, R, T, I, V, S, C, Y, K, F and G;

X10 is selected from K, Q, Y, T, F, L, W, M, A, E, G, and S;

X14 is selected from I, L, F, S, R, Y, Q, W, D, K and H (e.g., I, L and F);

X15 is selected from D, S, I, N, E, A, H, F, L, Q, M, G, Y and V;

X19 is selected from T, V, C, E, S and A (e.g., T and V);

X20 is selected from R, F, T, W, E, L, N, C, K, V, S, Q, I, Y, H and A;

X21 is selected from S, P, R, K, N, A, H, Q, G and L;

X22 is selected from D, G, T, N, S, K, A, I, E, L, Q, R and Y; and

X23 is selected from K, V, A, E, Y, I, C, L, S, T, G, K, M, D and F. In an embodiment, the HNH-like domain differs from a sequence of SEQ ID NO: 18 by 1, 2, 3, 4, or 5 residues.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an HNH- like domain comprising an amino acid sequence of formula VII:

X1-V-X3-H-I-V-P-X6-S-X8-X9-X10-D-D-S-X14-X15-N-K-V-L-T-X20-X21-X22- X23-N (SEQ ID NO: 19),

wherein

XI is selected from D and E;

X3 is selected from D and E;

X6 is selected from Q, H, R, K, Y, I, L and W;

X8 is selected from F, L, V, K, Y, M, I, R, A, E, D and Q (e.g., F);

X9 is selected from L, R, T, I, V, S, C, Y, K, F and G;

X10 is selected from K, Q, Y, T, F, L, W, M, A, E, G, and S;

X14 is selected from I, L, F, S, R, Y, Q, W, D, K and H (e.g., I, L and F);

X15 is selected from D, S, I, N, E, A, H, F, L, Q, M, G, Y and V;

X20 is selected from R, F, T, W, E, L, N, C, K, V, S, Q, I, Y, H and A;

X21 is selected from S, P, R, K, N, A, H, Q, G and L;

X22 is selected from D, G, T, N, S, K, A, I, E, L, Q, R and Y; and

X23 is selected from K, V, A, E, Y, I, C, L, S, T, G, K, M, D and F.

In an embodiment, the HNH-like domain differs from a sequence of SEQ ID NO: 19 by 1, 2, 3, 4, or 5 residues.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an HNH- like domain having an amino acid sequence of formula VIII:

D-X2-D-H-I-X5-P-Q-X7-F-X9-X10-D-X12-S-I-D-N-X16-V-L-X19-X20-S-X22- X23-N (SEQ ID NO:20),

wherein

X2 is selected from I and V;

X5 is selected from I and V;

X7 is selected from A and S;

X9 is selected from I and L;

X10 is selected from K and T;

X12 is selected from D and N;

X16 is selected from R, K and L; X19 is selected from T and V;

X20 is selected from S and R; X22 is selected from K, D and A; and

X23 is selected from E, K, G and N (e.g., the eaCas9 molecule or eaCas9 polypeptide can comprise an HNH-like domain as described herein).

In an embodiment, the HNH-like domain differs from a sequence of SEQ ID NO: 20 by as many as 1 but no more than 2, 3, 4, or 5 residues.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises the amino acid sequence of formula IX:

L-Y- Y-L-Q-N-G-Xl ' -D-M-Y-X2' -X3 ' -X4' -X5 ' -L-D-I— X6' -XT -L-S-X8 ' -Y-Z-N- R-X9'-K-X10'-D-X11'-V-P (SEQ ID NO: 21),

wherein

XI ' is selected from K and R;

X2' is selected from V and T;

X3' is selected from G and D;

X4' is selected from E, Q and D;

X5' is selected from E and D;

X6' is selected from D, N and H;

X7' is selected from Y, R and N;

X8' is selected from Q, D and N; X9' is selected from G and E;

X10' is selected from S and G;

XI 1 ' is selected from D and N; and

Z is an HNH-like domain, e.g., as described above.

In an embodiment, the eaCas9 molecule or eaCas9 polypeptide comprises an amino acid sequence that differs from a sequence of SEQ ID NO:21 by as many as 1 but no more than 2, 3, 4, or 5 residues.

In an embodiment, the HNH-like domain differs from a sequence of an HNH-like domain disclosed herein, e.g., in Figs. 5A-5C or Figs. 7A-7B, as many as 1 but no more than

2, 3, 4, or 5 residues. In an embodiment, 1 or both of the highly conserved residues identified in Figs. 5A-5C or Figs. 7A-7B are present.

In an embodiment, the HNH -like domain differs from a sequence of an HNH-like domain disclosed herein, e.g., in Figs. 6A-6B or Figs. 7A-7B, as many as 1 but no more than

2, 3, 4, or 5 residues. In an embodiment, 1, 2, all 3 of the highly conserved residues identified in Figs. 6A-6B or Figs. 7A-7B are present. Cas9 Activities

Nuclease and Helicase Activities

In an embodiment, the Cas9 molecule or Cas9 polypeptide is capable of cleaving a target nucleic acid molecule. Typically wild type Cas9 molecules cleave both strands of a target nucleic acid molecule. Cas9 molecules and Cas9 polypeptides can be engineered to alter nuclease cleavage (or other properties), e.g., to provide a Cas9 molecule or Cas9 peolypeptide which is a nickase, or which lacks the ability to cleave target nucleic acid. A Cas9 molecule or Cas9 polypeptide that is capable of cleaving a target nucleic acid molecule is referred to herein as an eaCas9 (an enzymatically active Cas9) molecule or eaCas9 polypeptide. In an embodiment, an eaCas9 molecule, comprises one or more of the following activities:

a nickase activity, i.e., the ability to cleave a single strand, e.g., the non- complementary strand or the complementary strand, of a nucleic acid molecule;

a double stranded nuclease activity, i.e., the ability to cleave both strands of a double stranded nucleic acid and create a double stranded break, which in an embodiment is the presence of two nickase activities;

an endonuclease activity;

an exonuclease activity; and

a helicase activity, i.e., the ability to unwind the helical structure of a double stranded nucleic acid.

In an embodiment, an enzymatically active Cas9 or an eaCas9 molecule or an eaCas9 polypeptide cleaves both DNA strands and results in a double stranded break. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide cleaves only one strand, e.g., the strand to which the gRNA hybridizes to, or the strand complementary to the strand the gRNA hybridizes with. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises cleavage activity associated with an HNH-like domain. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises cleavage activity associated with an N-terminal RuvC-like domain. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises cleavage activity associated with an HNH-like domain and cleavage activity associated with an N-terminal RuvC-like domain. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an active, or cleavage competent, HNH-like domain and an inactive, or cleavage incompetent, N-terminal RuvC-like domain. In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an inactive, or cleavage incompetent, HNH-like domain and an active, or cleavage competent, N-terminal RuvC-like domain. Some Cas9 molecules or Cas9 polypeptides have the ability to interact with a gRNA molecule, and in conjunction with the gRNA molecule localize to a core target domain, but are incapable of cleaving the target nucleic acid, or incapable of cleaving at efficient rates. Cas9 molecules having no, or no substantial, cleavage activity are referred to herein as an eiCas9 molecule or eiCas9 polypeptide. For example, an eiCas9 molecule or eiCas9 polypeptide can lack cleavage activity or have substantially less, e.g., less than 20, 10, 5, 1 or 0.1 % of the cleavage activity of a reference Cas9 molecule or eiCas9 polypeptide, as measured by an assay described herein. Targeting and PAMs

A Cas9 molecule or Cas9 polypeptide, is a polypeptide that can interact with a guide RNA (gRNA) molecule and, in concert with the gRNA molecule, localizes to a site which comprises a target domain and PAM sequence.

In an embodiment, the ability of an eaCas9 molecule or eaCas9 polypeptide to interact with and cleave a target nucleic acid is PAM sequence dependent. A PAM sequence is a sequence in the target nucleic acid. In an embodiment, cleavage of the target nucleic acid occurs upstream from the PAM sequence. EaCas9 molecules from different bacterial species can recognize different sequence motifs (e.g., PAM sequences). In an embodiment, an eaCas9 molecule of S. pyogenes recognizes the sequence motif NGG and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. See, e.g., Mali et al. , SCIENCE 2013; 339(6121): 823-826. In an embodiment, an eaCas9 molecule of S. thermophilus recognizes the sequence motif NGGNG and NNAGAAW (W \ or T) and directs cleavage of a core target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from these sequences. See, e.g., Horvath et al., SCIENCE 2010;

327(5962): 167-170, and Deveau et al., J BACTERIOL 2008; 190(4): 1390-1400. In an embodiment, an eaCas9 molecule of S. mutans recognizes the sequence motif NGG and/or NAAR (R = A or G) and directs cleavage of a core target nucleic acid sequence 1 to 10, e.g., 3 to 5 base pairs, upstream from this sequence. See, e.g., Deveau et al., J BACTERIOL 2008; 190(4): 1390-1400. In an embodiment, an eaCas9 molecule of S. aureus recognizes the sequence motif NNGRR (R = A or G) and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. In an embodiment, an eaCas9 molecule of S. aureus recognizes the sequence motif NNGRRN (R = A or G) and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. In an embodiment, an eaCas9 molecule of S. aureus recognizes the sequence motif NNGRRT (R = A or G) and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. In an embodiment, an eaCas9 molecule of S. aureus recognizes the sequence motif NNGRRV (R = A or G, V = A, G or C) and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. In an embodiment, an eaCas9 molecule of Neisseria meningitidis recognizes the sequence motif NNNNGATT or NNNGCTT and directs cleavage of a target nucleic acid sequence 1 to 10, e.g., 3 to 5, base pairs upstream from that sequence. See, e.g., Hou et al., PNAS Early Edition 2013, 1-6. The ability of a Cas9 molecule to recognize a PAM sequence can be determined, e.g., using a transformation assay described in Jinek et al., SCIENCE 2012 337:816. In the aforementioned embodiments, N can be any nucleotide residue, e.g., any of A, G, C or T.

As is discussed herein, Cas9 molecules can be engineered to alter the PAM specificity of the Cas9 molecule.

Exemplary naturally occurring Cas9 molecules are described in Chylinski et al., RNA BIOLOGY 2013 10:5, 727-737. Such Cas9 molecules include Cas9 molecules of a cluster 1 bacterial family, cluster 2 bacterial family, cluster 3 bacterial family, cluster 4 bacterial family, cluster 5 bacterial family, cluster 6 bacterial family, a cluster 7 bacterial family, a cluster 8 bacterial family, a cluster 9 bacterial family, a cluster 10 bacterial family, a cluster 11 bacterial family, a cluster 12 bacterial family, a cluster 13 bacterial family, a cluster 14 bacterial fami iy. a cluster 15 bacterial fami iy. a cluster 16 bacterial fami iy. a cluster 17 bacterial fami iy. a cluster 18 bacterial fami iy. a cluster 19 bacterial fami iy. a cluster 20 bacterial fami iy. a cluster 21 bacterial fami iy. a cluster 22 bacterial fami iy. a cluster 23 bacterial fami iy. a cluster 24 bacterial fami iy. a cluster 25 bacterial fami iy. a cluster 26 bacterial fami iy. a cluster 27 bacterial fami iy. a cluster 28 bacterial fami iy. a cluster 29 bacterial fami iy. a cluster 30 bacterial fami iy. a cluster 31 bacterial fami iy. a cluster 32 bacterial fami iy. a cluster 33 bacterial fami iy. a cluster 34 bacterial fami iy. a cluster 35 bacterial fami iy. a cluster 36 bacterial fami iy. a cluster 37 bacterial fami iy. a cluster 38 bacterial fami iy. a cluster 39 bacterial fami iy. a cluster 40 bacterial fami iy. a cluster 41 bacterial fami iy. a cluster 42 bacterial fami iy. a cluster 43 bacterial fami iy. a cluster 44 bacterial fami iy. a cluster 45 bacterial fami iy. a cluster 46 bacterial fami iy. a cluster 47 bacterial fami iy. a cluster 48 bacterial fami iy. a cluster 49 bacterial fami iy. a cluster 50 bacterial fami iy. a cluster 51 bacterial fami iy. a cluster 52 bacterial fami iy. a cluster 53 bacterial fami iy. a cluster 54 bacterial fami iy. a cluster 55 bacterial fami iy. a cluster 56 bacterial fami iy. a cluster 57 bacterial fami iy. a cluster 58 bacterial fami iy. a cluster 59 bacterial family, a cluster 60 bacterial family, a cluster 61 bacterial family, a cluster 62 bacterial family, a cluster 63 bacterial family, a cluster 64 bacterial family, a cluster 65 bacterial family, a cluster 66 bacterial family, a cluster 67 bacterial family, a cluster 68 bacterial family, a cluster 69 bacterial family, a cluster 70 bacterial family, a cluster 71 bacterial family, a cluster 72 bacterial family, a cluster 73 bacterial family, a cluster 74 bacterial family, a cluster 75 bacterial family, a cluster 76 bacterial family, a cluster 77 bacterial family, or a cluster 78 bacterial family.

Exemplary naturally occurring Cas9 molecules include a Cas9 molecule of a cluster 1 bacterial family. Examples include a Cas9 molecule of: S. pyogenes (e.g., strain SF370, MGAS10270, MGAS10750, MGAS2096, MGAS315, MGAS5005, MGAS6180,

MGAS9429, NZ131 and SSI-1), 5". thermophilus (e.g., strain LMD-9), 5". pseudoporcinus (e.g., strain SPIN 20026), S. mutans (e.g., strain UA159, NN2025), S. macacae (e.g., strain NCTC11558), S. gallolyticus (e.g., strain UCN34, ATCC BAA-2069), S. equines (e.g., strain ATCC 9812, MGCS 124), S. dysdalactiae (e.g., strain GGS 124), S. bovis (e.g., strain ATCC 700338), S. anginosus (e.g., strain F0211), S. agalactiae (e.g., strain NEM316, A909), Listeria monocytogenes (e.g., strain F6854), Listeria innocua (L. innocua, e.g., strain Clipl l262), Enterococcus italicus (e.g., strain DSM 15952), or Enterococcus faecium (e.g., strain 1,231,408). Additional exemplary Cas9 molecules are a Cas9 molecule of Neisseria meningitidis (Hou et al., PNAS Early Edition 2013, 1-6 and a S. aureus cas9 molecule.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence:

having 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology with;

differs at no more than, 2, 5, 10, 15, 20, 30, or 40% of the amino acid residues when compared with;

differs by at least 1, 2, 5, 10 or 20 amino acids, but by no more than 100, 80, 70, 60, 50, 40 or 30 amino acids from; or

is identical to any Cas9 molecule sequence described herein, or a naturally occurring Cas9 molecule sequence, e.g., a Cas9 molecule from a species listed herein or described in Chylinski et al, RNA BIOLOGY 2013 10:5, 727-737; Hou et al , PNAS Early Edition 2013, 1- 6; SEQ ID NO: 1-4. In an embodiment, the Cas9 molecule or Cas9 polypeptide comprises one or more of the following activities: a nickase activity; a double stranded cleavage activity (e.g., an endonuclease and/or exonuclease activity); a helicase activity; or the ability, together with a gRNA molecule, to localize to a target nucleic acid. In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises any of the amino acid sequence of the consensus sequence of Figs. 2A-2G, wherein "*" indicates any amino acid found in the corresponding position in the amino acid sequence of a Cas9 molecule or Cas9 polypeptide of S. pyogenes, S. thermophilus, S. mutans and L. innocua, and "-" indicates any amino acid. In an embodiment, a Cas9 molecule differs from the sequence of the consensus sequence of Figs. 2A-2G by at least 1, but no more than 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acid residues. In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises the amino acid sequence of SEQ ID NO:7 of Figs. 7A-7B, wherein "*" indicates any amino acid found in the corresponding position in the amino acid sequence of a Cas9 molecule of S. pyogenes, or N. meningitidis, "-" indicates any amino acid, and "-" indicates any amino acid or absent. In an embodiment, a Cas9 molecule or Cas9 polypeptide differs from the sequence of SEQ ID NO:6 or 7 disclosed in Figs. 7A-7B by at least 1, but no more than 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acid residues.

A comparison of the sequence of a number of Cas9 molecules or Cas9 polypeptide indicate that certain regions are conserved. These are identified below as:

region 1 ( residuesl to 180, or in the case of region 1 'residues 120 to 180) region 2 ( residues360 to 480);

region 3 ( residues 660 to 720);

region 4 ( residues 817 to 900); and

region 5 ( residues 900 to 960);

In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises regions 1-5, together with sufficient additional Cas9 molecule sequence to provide a biologically active molecule, e.g., a Cas9 molecule having at least one activity described herein. In an embodiment, each of regions 1-5, independently, have 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology with the corresponding residues of a Cas9 molecule or Cas9 polypeptide described herein, e.g., a sequence from Figs. 2A-2G or from Figs. 7A- 7B.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence referred to as region 1:

having 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology with amino acids 1-180 (the numbering is according to the motif sequence in Figs. 2A-2G; 52% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes;

differs by at least 1, 2, 5, 10 or 20 amino acids but by no more than 90, 80, 70, 60, 50, 40 or 30 amino acids from amino acids 1-180 of the amino acid sequence of Cas9 of S.

pyogenes, S. thermophilus, S. mutans or Listeria innocua; or

is identical to 1-180 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence referred to as region :

having 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology with amino acids 120-180 (55% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, .V. mutans or L. innocua;

differs by at least 1, 2, or 5 amino acids but by no more than 35, 30, 25, 20 or 10 amino acids from amino acids 120-180 of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or /.. innocua ; or

is identical to 120-180 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence referred to as region 2:

having 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% homology with amino acids 360-480 (52% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua;

differs by at least 1, 2, or 5 amino acids but by no more than 35, 30, 25, 20 or 10 amino acids from amino acids 360-480 of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or /.. innocua; or

is identical to 360-480 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence referred to as region 3:

having 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% homology with amino acids 660-720 (56% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or L. innocua;

differs by at least 1, 2, or 5 amino acids but by no more than 35, 30, 25, 20 or 10 amino acids from amino acids 660-720 of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or /.. innocua; or

is identical to 660-720 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or easCas9 polypeptide, comprises an amino acid sequence referred to as region 4:

having 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% homology with amino acids 817-900 (55% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua;

differs by at least 1, 2, or 5 amino acids but by no more than 35, 30, 25, 20 or 10 amino acids from amino acids 817-900 of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or /.. innocua; or

is identical to 817-900 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

In an embodiment, a Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, comprises an amino acid sequence referred to as region 5:

having 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% homology with amino acids 900-960 (60% of residues in the four Cas9 sequences in Figs. 2A-2G are conserved) of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua;

differs by at least 1, 2, or 5 amino acids but by no more than 35, 30, 25, 20 or 10 amino acids from amino acids 900-960 of the amino acid sequence of Cas9 of S. pyogenes, S. thermophilus, S. mutans or /.. innocua; or

is identical to 900-960 of the amino acid sequence of Cas9 of S. pyogenes, S.

thermophilus, S. mutans or /.. innocua.

Engineered or Altered Cas9 Molecules and Cas9 Polypeptides

Cas9 molecules and Cas9 polypeptides described herein, e.g., naturally occurring Cas9 molecules can possess any of a number of properties, including: nickase activity, nuclease activity (e.g., endonuclease and/or exonuc lease activity); helicase activity; the ability to associate functionally with a gRNA molecule; and the ability to target (or localize to) a site on a nucleic acid (e.g., PAM recognition and specificity). In an embodiment, a Cas9 molecule or Cas9 polypeptide can include all or a subset of these properties. In typical embodiments, a Cas9 molecule or Cas9 polypeptide have the ability to interact with a gRNA molecule and, in concert with the gRNA molecule, localize to a site in a nucleic acid. Other activities, e.g., PAM specificity, cleavage activity, or helicase activity can vary more widely in Cas9 molecules and Cas9 polypeptides.

Cas9 molecules include engineered Cas9 molecules and engineered Cas9

polypeptides (engineered, as used in this context, means merely that the Cas9 molecule or Cas9 polypeptide differs from a reference sequences, and implies no process or origin limitation). An engineered Cas9 molecule or Cas9 polypeptide can comprise altered enzymatic properties, e.g., altered nuclease activity, (as compared with a naturally occurring or other reference Cas9 molecule) or altered helicase activity. As discussed herein, an engineered Cas9 molecule or Cas9 polypeptide can have nickase activity (as opposed to double strand nuclease activity). In an embodiment an engineered Cas9 molecule or Cas9 polypeptide can have an alteration that alters its size, e.g., a deletion of amino acid sequence that reduces its size, e.g., without significant effect on one or more, or any Cas9 activity. In an embodiment, an engineered Cas9 molecule or Cas9 polypeptide can comprise an alteration that affects PAM recognition. E.g., an engineered Cas9 molecule can be altered to recognize a PAM sequence other than that recognized by the endogenous wild- type PI domain. In an embodiment, a Cas9 molecule or Cas9 polypeptide can differ in sequence from a naturally occurring Cas9 molecule but not have significant alteration in one or more Cas9 activities.

Cas9 molecules or Cas9 polypeptides with desired properties can be made in a number of ways, e.g., by alteration of a parental, e.g., naturally occurring Cas9 molecules or Cas9 polypeptides to provide an altered Cas9 molecule or Cas9 polypeptides having a desired property. For example, one or more mutations or differences relative to a parental Cas9 molecule can be introduced. Such mutations and differences comprise: substitutions (e.g., conservative substitutions or substitutions of non-essential amino acids); insertions; or deletions. In an embodiment, a Cas9 molecule or Cas9 polypeptide can comprises one or more mutations or differences, e.g., at least 1, 2, 3, 4, 5, 10, 15, 20, 30, 40 or 50 mutations, but less than 200, 100, or 80 mutations relative to a reference, e.g., a parental, Cas9 molecule.

In an embodiment, a mutation or mutations do not have a substantial effect on a Cas9 activity, e.g. a Cas9 activity described herein. In an embodiment, a mutation or mutations have a substantial effect on a Cas9 activity, e.g. a Cas9 activity described herein.

Non-Cleaving and Modified-Cleavage Cas9 Molecules and Cas9 Polypeptides

In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises a cleavage property that differs from naturally occurring Cas9 molecules, e.g., that differs from the naturally occurring Cas9 molecule having the closest homology. For example, a Cas9 molecule or Cas9 polypeptide can differ from naturally occurring Cas9 molecules, e.g., a Cas9 molecule of S. pyogenes, as follows: its ability to modulate, e.g., decreased or increased, cleavage of a double stranded nucleic acid (endonuc lease and/or exonuclease activity), e.g., as compared to a naturally occurring Cas9 molecule (e.g., a Cas9 molecule of S. pyogenes); its ability to modulate, e.g., decreased or increased, cleavage of a single strand of a nucleic acid, e.g., a non-complementary strand of a nucleic acid molecule or a complementary strand of a nucleic acid molecule (nickase activity) , e.g., as compared to a naturally occurring Cas9 molecule (e.g., a Cas9 molecule of S. pyogenes); or the ability to cleave a nucleic acid molecule, e.g., a double stranded or single stranded nucleic acid molecule, can be eliminated.

Modified Cleavage eaCas9 Molecules and eaCas9 Polypeptides

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises one or more of the following activities: cleavage activity associated with an N-terminal RuvC-like domain; cleavage activity associated with an HNH-like domain; cleavage activity associated with an HNH-like domain and cleavage activity associated with an N-terminal RuvC-like domain.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an active, or cleavage competent, HNH-like domain (e.g., an HNH-like domain described herein, e.g., SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 or SEQ ID NO: 21) and an inactive, or cleavage incompetent, N-terminal RuvC-like domain. An exemplary inactive, or cleavage incompetent N-terminal RuvC-like domain can have a mutation of an aspartic acid in an N-terminal RuvC-like domain, e.g., an aspartic acid at position 9 of the consensus sequence disclosed in Figs. 2A-2G or an aspartic acid at position 10 of SEQ ID NO: 7, e.g., can be substituted with an alanine. In an embodiment, the eaCas9 molecule or eaCas9 polypeptide differs from wild type in the N-terminal RuvC-like domain and does not cleave the target nucleic acid, or cleaves with significantly less efficiency, e.g., less than 20, 10, 5, 1 or .1 % of the cleavage activity of a reference Cas9 molecule, e.g., as measured by an assay described herein. The reference Cas9 molecule can by a naturally occurring unmodified Cas9 molecule, e.g., a naturally occurring Cas9 molecule such as a Cas9 molecule of S.

pyogenes, or S. thermophilus. In an embodiment, the reference Cas9 molecule is the naturally occurring Cas9 molecule having the closest sequence identity or homology.

In an embodiment, an eaCas9 molecule or eaCas9 polypeptide comprises an inactive, or cleavage incompetent, HNH domain and an active, or cleavage competent, N-terminal RuvC-like domain (e.g., a RuvC-like domain described herein, e.g., SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, or SEQ ID NO: 16). Exemplary inactive, or cleavage incompetent HNH- like domains can have a mutation at one or more of: a histidine in an HNH-like domain, e.g., a histidine shown at position 856 of the consensus sequence disclosed in Figs. 2A-2G, e.g., can be substituted with an alanine; and one or more asparagines in an HNH-like domain, e.g., an asparagine shown at position 870 of the consensus sequence disclosed in Figs. 2A-2G and/or at position 879 of the consensus sequence disclosed in Figs. 2A-2G, e.g., can be substituted with an alanine. In an embodiment, the eaCas9 differs from wild type in the HNH-like domain and does not cleave the target nucleic acid, or cleaves with significantly less efficiency, e.g., less than 20, 10, 5, 1 or 0.1% of the cleavage activity of a reference Cas9 molecule, e.g., as measured by an assay described herein. The reference Cas9 molecule can by a naturally occurring unmodified Cas9 molecule, e.g., a naturally occurring Cas9 molecule such as a Cas9 molecule of S. pyogenes, or S. thermophilus. In an embodiment, the reference Cas9 molecule is the naturally occurring Cas9 molecule having the closest sequence identity or homology.

Alterations in the Ability to Cleave One or Both Strands of a Target Nucleic Acid In an embodiment, exemplary Cas9 activities comprise one or more of PAM specificity, cleavage activity, and helicase activity. A mutation(s) can be present, e.g., in one or more RuvC-like domain, e.g., an N-terminal RuvC-like domain; an HNH-like domain; a region outside the RuvC-like domains and the HNH-like domain. In some embodiments, a mutation(s) is present in a RuvC-like domain, e.g., an N-terminal RuvC-like domain. In some embodiments, a mutation(s) is present in an HNH-like domain. In some embodiments, mutations are present in both a RuvC-like domain, e.g., an N-terminal RuvC-like domain and an HNH-like domain.

Exemplary mutations that may be made in the RuvC domain or HNH domain with reference to the S. pyogenes sequence include: D10A, E762A, H840A, N854A, N863A and/or D986A.

In an embodiment, a Cas9 molecule or Cas9 polypeptide is an eiCas9 molecule or eiCas9 polypeptide comprising one or more differences in a RuvC domain and/or in an HNH domain as compared to a reference Cas9 molecule, and the eiCas9 molecule or eiCas9 polypeptide does not cleave a nucleic acid, or cleaves with significantly less efficiency than does wildype, e.g., when compared with wild type in a cleavage assay, e.g., as described herein, cuts with less than 50, 25, 10, or 1% of a reference Cas9 molecule, as measured by an assay described herein.

Whether or not a particular sequence, e.g., a substitution, may affect one or more activity, such as targeting activity, cleavage activity, etc., can be evaluated or predicted, e.g., by evaluating whether the mutation is conservative or by the method described in Section IV. In an embodiment, a "non-essential" amino acid residue, as used in the context of a Cas9 molecule, is a residue that can be altered from the wild-type sequence of a Cas9 molecule, e.g., a naturally occurring Cas9 molecule, e.g., an eaCas9 molecule, without abolishing or more preferably, without substantially altering a Cas9 activity (e.g., cleavage activity), whereas changing an "essential" amino acid residue results in a substantial loss of activity (e.g., cleavage activity).

In an embodiment, a Cas9 molecule or Cas9 polypeptide comprises a cleavage property that differs from naturally occurring Cas9 molecules, e.g., that differs from the naturally occurring Cas9 molecule having the closest homology. For example, a Cas9 molecule or Cas9 polypeptide can differ from naturally occurring Cas9 molecules, e.g., a Cas9 molecule of S aureus, S. pyogenes, or C. jejuni as follows: its ability to modulate, e.g., decreased or increased, cleavage of a double stranded break (endonuclease and/or exonuclease activity), e.g., as compared to a naturally occurring Cas9 molecule (e.g., a Cas9 molecule of S aureus, S. pyogenes, or C. jejuni); its ability to modulate, e.g., decreased or increased, cleavage of a single strand of a nucleic acid, e.g., a non-complimentary strand of a nucleic acid molecule or a complementary strand of a nucleic acid molecule (nickase activity), e.g., as compared to a naturally occurring Cas9 molecule (e.g., a Cas9 molecule of S aureus, S. pyogenes, or C. jejuni); or the ability to cleave a nucleic acid molecule, e.g., a double stranded or single stranded nucleic acid molecule, can be eliminated.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide is an eaCas9 molecule or eaCas9 polypeptide comprising one or more of the following activities: cleavage activity associated with a RuvC domain; cleavage activity associated with an HNH domain; cleavage activity associated with an HNH domain and cleavage activity associated with a RuvC domain.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide is an eiCas9 molecule or eiCas9 polypeptide which does not cleave a nucleic acid molecule (either double stranded or single stranded nucleic acid molecules) or cleaves a nucleic acid molecule with significantly less efficiency, e.g., less than 20, 10, 5, 1 or 0.1% of the cleavage activity of a reference Cas9 molecule, e.g., as measured by an assay described herein. The reference Cas9 molecule can be a naturally occurring unmodified Cas9 molecule, e.g., a naturally occurring Cas9 molecule such as a Cas9 molecule of S. pyogenes, S. thermophilus, S. aureus, C. jejuni or N. meningitidis. In an embodiment, the reference Cas9 molecule is the naturally occurring Cas9 molecule having the closest sequence identity or homology. In an embodiment, the eiCas9 molecule or eiCas9 polypeptide lacks substantial cleavage activity associated with a RuvC domain and cleavage activity associated with an ΗΝΗ domain.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide is an eaCas9 molecule or eaCas9 polypeptides comprising the fixed amino acid residues of S. pyogenes shown in the consensus sequence disclosed in Figs. 2A-2G, and has one or more amino acids that differ from the amino acid sequence of S. pyogenes (e.g., has a substitution) at one or more residue (e.g., 2, 3, 5, 10, 15, 20, 30, 50, 70, 80, 90, 100, 200 amino acid residues) represented by an "-" in the consensus sequence disclosed in Figs. 2A-2G or SEQ ID NO:7.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptides comprises a sequence in which:

the sequence corresponding to the fixed sequence of the consensus sequence disclosed in Figs. 2A-2Gdiffers at no more than 1, 2, 3, 4, 5, 10, 15, or 20% of the fixed residues in the consensus sequence disclosed in Figs. 2A-2G;

the sequence corresponding to the residues identified by "*" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, or 40% of the "*" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. pyogenes Cas9 molecule; and,

the sequence corresponding to the residues identified by "-" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 5, 10, 15, 20, 25, 30, 35, 40, 45, 55, or 60% of the "-" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. pyogenes Cas9 molecule.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptides is an eaCas9 molecule or eaCas9 polypeptidecomprising the fixed amino acid residues of S. thermophilus shown in the consensus sequence disclosed in Figs. 2A-2G, and has one or more amino acids that differ from the amino acid sequence of S. thermophilus (e.g., has a substitution) at one or more residue (e.g., 2, 3, 5, 10, 15, 20, 30, 50, 70, 80, 90, 100, 200 amino acid residues) represented by an "-" in the consensus sequence disclosed in Figs. 2A-2G. In an

embodiment

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide comprises a sequence in which: the sequence corresponding to the fixed sequence of the consensus sequence disclosed in Figs. 2A-2G differs at no more than 1, 2, 3, 4, 5, 10, 15, or 20% of the fixed residues in the consensus sequence disclosed in Figs. 2A-2G;

the sequence corresponding to the residues identified by "*" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, or 40% of the "*" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. thermophilus Cas9 molecule; and,

the sequence corresponding to the residues identified by "-" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 5, 10, 15, 20, 25, 30, 35, 40, 45, 55, or 60% of the "-" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. thermophilus Cas9 molecule.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide is an eaCas9 molecule or ea Cas9 polypeptide comprising the fixed amino acid residues of S. mutans shown in the consensus sequence disclosed in Figs. 2A-2G, and has one or more amino acids that differ from the amino acid sequence of S. mutans (e.g., has a substitution) at one or more residue (e.g., 2, 3, 5, 10, 15, 20, 30, 50, 70, 80, 90, 100, 200 amino acid residues) represented by an "-" in the consensus sequence disclosed in Figs. 2A-2G.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide comprises a sequence in which:

the sequence corresponding to the fixed sequence of the consensus sequence disclosed in Figs. 2A-2G differs at no more than 1, 2, 3, 4, 5, 10, 15, or 20% of the fixed residues in the consensus sequence disclosed in Figs. 2A-2G;

the sequence corresponding to the residues identified by "*" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, or 40% of the "*" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. mutans Cas9 molecule; and,

the sequence corresponding to the residues identified by "-" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 5, 10, 15, 20, 25, 30, 35, 40, 45, 55, or 60% of the "-" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an S. mutans Cas9 molecule.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide is an eaCas9 molecule or eaCas9 polypeptide comprising the fixed amino acid residues of L. innocula shown in the consensus sequence disclosed in Figs. 2A-2G, and has one or more amino acids that differ from the amino acid sequence of L. innocula (e.g., has a substitution) at one or more residue (e.g., 2, 3, 5, 10, 15, 20, 30, 50, 70, 80, 90, 100, 200 amino acid residues) represented by an "-"in the consensus sequence disclosed in Figs. 2A-2G.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide comprises a sequence in which:

the sequence corresponding to the fixed sequence of the consensus sequence disclosed in Figs. 2A-2G differs at no more than 1, 2, 3, 4, 5, 10, 15, or 20% of the fixed residues in the consensus sequence disclosed in Figs. 2A-2G;

the sequence corresponding to the residues identified by "*" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, or 40% of the "*" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an L. innocula Cas9 molecule; and,

the sequence corresponding to the residues identified by "-" in the consensus sequence disclosed in Figs. 2A-2G differ at no more than 5, 10, 15, 20, 25, 30, 35, 40, 45, 55, or 60% of the "-" residues from the corresponding sequence of naturally occurring Cas9 molecule, e.g., an L. innocula Cas9 molecule.

In an embodiment, the altered Cas9 molecule or Cas9 polypeptide, e.g., an eaCas9 molecule or eaCas9 polypeptide, can be a fusion, e.g., of two of more different Cas9 molecules, e.g., of two or more naturally occurring Cas9 molecules of different species. For example, a fragment of a naturally occurring Cas9 molecule of one species can be fused to a fragment of a Cas9 molecule of a second species. As an example, a fragment of a Cas9 molecule of S. pyoge es comprising an N-terminal RuvC-like domain can be fused to a fragment of a Cas9 molecule of a species other than S. pyogenes (e.g., S. thermophilus) comprising an HNH-like domain. Cas9 Molecules and Cas9 polypeptides with Altered PAM Recognition or no PAM

Recognition

Naturally occurring Cas9 molecules can recognize specific PAM sequences, for example, the PAM recognition sequences described above for S. pyogenes, S. thermophiles, S. mutans, S. aureus and N. meningitidis.

In an embodiment, a Cas9 molecule or Cas9 polypeptide has the same PAM specificities as a naturally occurring Cas9 molecule. In other embodiments, a Cas9 molecule or Cas9 polypeptide has a PAM specificity not associated with a naturally occurring Cas9 molecule, or a PAM specificity not associated with the naturally occurring Cas9 molecule to which it has the closest sequence homology. For example, a naturally occurring Cas9 molecule or Cas9 polypeptide can be altered, e.g., to alter PAM recognition, e.g., to alter the PAM sequence that the Cas9 molecule recognizes to decrease off target sites and/or improve specificity; or eliminate a PAM recognition requirement. In an embodiment, a Cas9 molecule or Cas9 polypeptide can be altered, e.g., to increase length of PAM recognition sequence and/or improve Cas9 specificity to high level of identity (e.g., 98%, 99% or 100% match between gRNA and a PAM sequence), e.g., to decrease off target sites and increase specificity. In an embodiment, the length of the PAM recognition sequence is at least 4, 5, 6, 7, 8, 9, 10 or 15 amino acids in length. In an embodiment, the Cas9 specificity requires at least 90%, 95%, 96%, 97%, 98%, 99% or more homology between the gRNA and the PAM sequence. Cas9 molecules or Cas9 polypeptide that recognize different PAM sequences and/or have reduced off-target activity can be generated using directed evolution. Exemplary methods and systems that can be used for directed evolution of Cas9 molecules are described, e.g., in Esvelt et al. NATURE 2011, 472(7344): 499-503. Candidate Cas9 molecules can be evaluated, e.g., by methods described in Section IV.

Alterations of the PI domain, which mediates PAM recognition, are discussed below.

Synthetic Cas9 Molecules and Cas9 Polypeptides with Altered PI Domains

Current genome-editing methods are limited in the diversity of target sequences that can be targeted by the PAM sequence that is recognized by the Cas9 molecule utilized. A synthetic Cas9 molecule (or Syn-Cas9 molecule), or synthetic Cas9 polypeptide (or Syn-Cas9 polypeptide), as that term is used herein, refers to a Cas9 molecule or Cas9 polypeptide that comprises a Cas9 core domain from one bacterial species and a functional altered PI domain, i.e., a PI domain other than that naturally associated with the Cas9 core domain, e.g., from a different bacterial species.

In an embodiment, the altered PI domain recognizes a PAM sequence that is different from the PAM sequence recognized by the naturally-occurring Cas9 from which the Cas9 core domain is derived. In an embodiment, the altered PI domain recognizes the same PAM sequence recognized by the naturally-occurring Cas9 from which the Cas9 core domain is derived, but with different affinity or specificity. A Syn-Cas9 molecule or Syn-Cas9 polypetide can be, respectively, a Syn-eaCas9 molecule or Syn-eaCas9 polypeptide or a Syn- eiCas9 molecule Syn-eiCas9 polypeptide.

An exemplary Syn-Cas9 molecule or Syn-Cas9 polypetide comprises:

a) a Cas9 core domain, e.g., a Cas9 core domain from Table 17 or 18, e.g., a S.

aureus, S. pyogenes, or C. jejuni Cas9 core domain; and b) an altered PI domain from a species X Cas9 sequence selected from Tables 20 and

21.

In an embodiment, the RKR motif (the PAM binding motif) of said altered PI domain comprises: differences at 1, 2, or 3 amino acid residues; a difference in amino acid sequence at the first, second, or third position; differences in amino acid sequence at the first and second positions, the first and third positions, or the second and third positions; as compared with the sequence of the RKR motif of the native or endogenous PI domain associated with the Cas9 core domain.

In an embodiment, the Cas9 core domain comprises the Cas9 core domain from a species X Cas9 from Table 17 and said altered PI domain comprises a PI domain from a species Y Cas9 from Table 17.

In an embodiment, the RKR motif of the species X Cas9 is other than the RKR motif of the species Y Cas9.

In an embodiment, the RKR motif of the altered PI domain is selected from XXY, XNG, and XNQ.

In an embodiment, the altered PI domain has at least 60, 70, 80, 90, 95, or 100% homology with the amino acid sequence of a naturally occurring PI domain of said species Y from Table 17.

In an embodiment, the altered PI domain differs by no more than 50, 40, 30, 25, 20, 15, 10, 5, 4, 3, 2, or 1 amino acid residue from the amino acid sequence of a naturally occurring PI domain of said second species from Table 17.

In an embodiment, the Cas9 core domain comprises a S. aureus core domain and altered PI domain comprises: an A. denitrificans PI domain; a C. jejuni PI domain; a H. mustelae PI domain; or an altered PI domain of species X PI domain, wherein species X is selected from Table 21.

In an embodiment, the Cas9 core domain comprises a S. pyogenes core domain and the altered PI domain comprises: an A. denitrificans PI domain; a C. jejuni PI domain; a H. mustelae PI domain; or an altered PI domain of species X PI domain, wherein species X is selected from Table 21.

In an embodiment, the Cas9 core domain comprises a C. jejuni core domain and the altered PI domain comprises: an A. denitrificans PI domain; a H. mustelae PI domain; or an altered PI domain of species X PI domain, wherein species X is selected from Table 21.

In an embodiment, the Cas9 molecule or Cas9 polypeptide further comprises a linker disposed between said Cas9 core domain and said altered PI domain. In an embodiment, the linker comprises: a linker described elsewhere herein disposed between the Cas9 core domain and the heterologous PI domain. Suitable linkers are further described in Section V.

Exemplary altered PI domains for use in Syn-Cas9 molecules are described in Tables 20 and 21. The sequences for the 83 Cas9 orthologs referenced in Tables 20 and 21 are provided in Table 17. Table 19 provides the Cas9 orthologs with known PAM sequences and the corresponding RKR motif.

In an embodiment, a Syn-Cas9 molecule or Syn-Cas9 polypeptide may also be size- optimized, e.g., the Syn-Cas9 molecule or Syn-Cas9 polypeptide comprises one or more deletions, and optionally one or more linkers disposed between the amino acid residues flanking the deletions. In an embodiment, a Syn-Cas9 molecule or Syn-Cas9 polypeptide comprises a REC deletion.

Size- Optimized Cas9 Molecules and Cas9 Polypeptides

Engineered Cas9 molecules and engineered Cas9 polypeptides described herein include a Cas9 molecule or Cas9 polypeptide comprising a deletion that reduces the size of the molecule while still retaining desired Cas9 properties, e.g., essentially native

conformation, Cas9 nuclease activity, and/or target nucleic acid molecule recognition.

Provided herein are Cas9 molecules or Cas9 polypeptides comprising one or more deletions and optionally one or more linkers, wherein a linker is disposed between the amino acid residues that flank the deletion. Methods for identifying suitable deletions in a reference Cas9 molecule, methods for generating Cas9 molecules with a deletion and a linker, and methods for using such Cas9 molecules will be apparent to one of ordinary skill in the art upon review of this document.

A Cas9 molecule, e.g., a S. aureus, S. pyogenes, or C. jejuni, Cas9 molecule, having a deletion is smaller, e.g., has reduced number of amino acids, than the corresponding naturally-occurring Cas9 molecule. The smaller size of the Cas9 molecules allows increased flexibility for delivery methods, and thereby increases utility for genome-editing. A Cas9 molecule or Cas9 polypeptide can comprise one or more deletions that do not substantially affect or decrease the activity of the resultant Cas9 molecules or Cas9 polypeptides described herein. Activities that are retained in the Cas9 molecules or Cas9 polypeptides comprising a deletion as described herein include one or more of the following:

a nickase activity, i.e., the ability to cleave a single strand, e.g., the non- complementary strand or the complementary strand, of a nucleic acid molecule; a double stranded nuclease activity, i.e., the ability to cleave both strands of a double stranded nucleic acid and create a double stranded break, which in an embodiment is the presence of two nickase activities;

an endonuclease activity;

an exonuclease activity;

a helicase activity, i.e., the ability to unwind the helical structure of a double stranded nucleic acid;

and recognition activity of a nucleic acid molecule, e.g., a target nucleic acid or a gRNA.

Activity of the Cas9 molecules or Cas9 polypeptides described herein can be assessed using the activity assays described herein or in the art.

Identifying regions suitable for deletion

Suitable regions of Cas9 molecules for deletion can be identified by a variety of methods. Naturally-occurring orthologous Cas9 molecules from various bacterial species, e.g., any one of those listed in Table 17, can be modeled onto the crystal structure of S. pyogenes Cas9 (Nishimasu et al., Cell, 156:935-949, 2014) to examine the level of conservation across the selected Cas9 orthologs with respect to the three-dimensional conformation of the protein. Less conserved or unconserved regions that are spatially located distant from regions involved in Cas9 activity, e.g., interface with the target nucleic acid molecule and/or gRNA, represent regions or domains are candidates for deletion without substantially affecting or decreasing Cas9 activity.

REC-Optimized Cas9 Molecules and Cas9 Polypeptides

A REC-optimized Cas9 molecule, or a REC-optimized Cas9 polypeptide, as that term is used herein, refers to a Cas9 molecule or Cas9 polypeptide that comprises a deletion in one or both of the REC2 domain and the REICT domain (collectively a REC deletion), wherein the deletion comprises at least 10% of the amino acid residues in the cognate domain. A REC-optimized Cas9 molecule or Cas9 polypeptide can be an eaCas9 molecule or eaCas9 polypetide, or an eiCas9 molecule or eiCas9 polypeptide. An exemplary REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide comprises:

a) a deletion selected from:

i) a REC2 deletion;

ii) a RECICT deletion; or iii) a REC 1 SUB deletion.

Optionally, a linker is disposed between the amino acid residues that flank the deletion. In an embodiment, a Cas9 molecule or Cas9 polypeptide includes only one deletion, or only two deletions. A Cas9 molecule or Cas9 polypeptide can comprise a REC2 deletion and a REClcr deletion. A Cas9 molecule or Cas9 polypeptide can comprise a REC2 deletion and a REC 1 SUB deletion.

Generally, the deletion will contain at least 10% of the amino acids in the cognate domain, e.g., a REC2 deletion will include at least 10% of the amino acids in the REC2 domain. A deletion can comprise: at least 10, 20, 30, 40, 50, 60, 70, 80, or 90% of the amino acid residues of its cognate domain; all of the amino acid residues of its cognate domain; an amino acid residue outside its cognate domain; a plurality of amino acid residues outside its cognate domain; the amino acid residue immediately N terminal to its cognate domain; the amino acid residue immediately C terminal to its cognate domain; the amino acid residue immediately N terminal to its cognate and the amino acid residue immediately C terminal to its cognate domain; a plurality of, e.g., up to 5, 10, 15, or 20, amino acid residues N terminal to its cognate domain; a plurality of, e.g., up to 5, 10, 15, or 20, amino acid residues C terminal to its cognate domain; a plurality of, e.g., up to 5, 10, 15, or 20, amino acid residues N terminal to to its cognate domain and a plurality of e.g., up to 5, 10, 15, or 20, amino acid residues C terminal to its cognate domain.

In an embodiment, a deletion does not extend beyond: its cognate domain; the N terminal amino acid residue of its cognate domain; the C terminal amino acid residue of its cognate domain.

A REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide can include a linker disposed between the amino acid residues that flank the deletion. Any linkers known in the art that maintain the conformation or native fold of the Cas9 molecule (thereby retaining Cas9 activity) can be used between the amino acid resides that flank a REC deletion in a REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide. Linkers for use in generating recombinant proteins, e.g., multi-domain proteins, are known in the art (Chen et al., Adv Drug Delivery Rev, 65: 1357-69, 2013).

In an embodiment, a REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide comprises an amino acid sequence that, other than any REC deletion and associated linker, has at least 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99, or 100% homology with the amino acid sequence of a naturally occurring Cas 9, e.g., a Cas9 molecule described in Table 17, e.g., a S. aureus Cas9 molecule, a S. pyogenes Cas9 molecule, or a C. jejuni Cas9 molecule.

In an embodiment, a a REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide comprises an amino acid sequence that, other than any REC deletion and associated linker, differs by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25, amino acid residues from the amino acid sequence of a naturally occurring Cas 9, e.g., a Cas9 molecule described in Table 17, e.g., a S. aureus Cas9 molecule, a S. pyogenes Cas9 molecule, or a C. jejuni Cas9 molecule.

In an embodiment, a REC-optimized Cas9 molecule or REC-optimized Cas9 polypeptide comprises an amino acid sequence that, other than any REC deletion and associate linker, differs by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25% of the, amino acid residues from the amino acid sequence of a naturally occurring Cas 9, e.g., a Cas9 molecule described in Table 17, e.g., a S. aureus Cas9 molecule, a S. pyogenes Cas9 molecule, or a C. jejuni Cas9 molecule.

For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. Methods of alignment of sequences for comparison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman, (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, (1970) J. Mol. Biol. 48:443, by the search for similarity method of Pearson and Lipman, (1988) Proc. Nat'l. Acad. Sci. USA 85:2444, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics

Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI), or by manual alignment and visual inspection (see, e.g., Brent et al., (2003) Current Protocols in Molecular Biology).

Two examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., (1977) Nuc. Acids Res. 25:3389-3402; and Altschul et al., (1990) J. Mol. Biol. 215:403-410, respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information.

The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller, (1988) Comput. Appl. Biosci. 4:11-17) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (1970) J. Mol. Biol. 48:444-453) algorithm which has been incorporated into the GAP program in the GCG software package (available at www.gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6.

Sequence information for exemplary REC deletions are provided for 83 naturally- occurring Cas9 orthologs in Table 17.

The amino acid sequences of exemplary Cas9 molecules from different bacterial species are shown below.

Table 17. Amino Acid Sequence of Cas9 Orthologs

17330 312

gil366983953lgblEHN59352.11

Fructobacillus fructosus KCTC SEQ ID NO: 168 314 147 488 571 76 488 571 76

3544 313

gil339625081lreflZP_08660870.

1

Catenibacterium mitsuokai SEQ ID NO: 173 318 146 511 594 78 511 594 78 DSM 15897 314

gil224543312lreflZP_03683851.

1

Finegoldia magna ATCC 29328 SEQ ID NO: 168 313 146 452 534 77 452 534 77 gill69823755lreflYP_00169136 315

6.1

CoriobacteriumglomeransPW2 SEQ ID NO: 175 318 144 511 592 82 511 592 82 gil328956315lreflYP_00437364 316

8.1

Eubacterium yurii ATCC 43715 SEQ ID NO: 169 310 142 552 633 76 552 633 76 gil306821691 lreflZP_07455288. 317

1

Peptoniphilus duerdenii ATCC SEQ ID NO: 171 311 141 535 615 76 535 615 76

BAA- 1640 318

gil304438954lreflZP_07398877.

1

Acidaminococcus sp. D21 SEQ ID NO: 167 306 140 511 591 75 511 591 75 gil227824983lreflZP_03989815. 319

1

Lactobacillus farciminis KCTC SEQ ID NO: 171 310 140 542 621 85 542 621 85

3681 320

gil336394882lreflZP_08576281.

1

Streptococcus sanguinis SK49 SEQ ID NO: 185 324 140 411 490 85 411 490 85 gil422884106lreflZP_16930555. 321

1

Coprococcus catus GD-7 SEQ ID NO: 172 310 139 556 634 76 556 634 76 gil291520705lemblCBK78998.1 322

1

Streptococcus mutans UA159 SEQ ID NO: 176 314 139 392 470 84 392 470 84 gil24379809lreflNP_721764.1l 323

Streptococcus pyogenes Ml SEQ ID NO: 176 314 139 523 600 82 523 600 82 GAS 324

gill3622193lgblAAK33936.1l

Streptococcus thermophilus SEQ ID NO: 176 314 139 481 558 81 481 558 81 LMD-9 325

gil 116628213 Irefl YP_820832.11

Fusobacteriumnucleatum SEQ ID NO: 171 308 138 537 614 76 537 614 76 ATCC49256 326

gil34762592lreflZP_00143587.1

1

Planococcus antarcticus DSM SEQ ID NO: 162 299 138 538 614 94 538 614 94

14505 327

gil389815359lreflZP_10206685.

1

Treponema denticola ATCC SEQ ID NO: 169 305 137 524 600 81 524 600 81 35405 328

gil42525843lref INP_970941.11

Solobacterium moorei F0204 SEQ ID NO: 179 314 136 544 619 77 544 619 77

329 gil320528778lreflZP_08029929.

1

Staphylococcus SEQ ID NO: 164 299 136 531 606 92 531 606 92 pseudintermedius ED99 330

gil323463801lgblADX75954.1l

Flavobacterium branchiophilum SEQ ID NO: 162 286 125 538 613 63 538 613 63

FL-15 331

gil347536497lreflYP_00484392

2.1

Ignavibacterium album JCM SEQ ID NO: 223 329 107 357 432 90 357 432 90 16511 332

gil385811609lrellYP_00584800

5.1

Bergeyella zoohelcum ATCC SEQ ID NO: 165 261 97 529 604 56 529 604 56

43767 333

gil423317190lreflZP_17295095.

1

Nitrobacter hamburgensis X14 SEQ ID NO: 169 253 85 536 611 48 536 611 48 gil92109262lreflYP_571550.1l 334

Odoribacter laneus YIT 12061 SEQ ID NO: 164 242 79 535 610 63 535 610 63 gil374384763lreflZP_09642280. 335

1

Legionella pneumophila str. SEQ ID NO: 164 239 76 402 476 67 402 476 67

Paris 336

gil54296138lreflYP_122507.1l

Bacteroides sp. 20 3 SEQ ID NO: 198 269 72 530 604 83 530 604 83 gil301311869lrellZP_07217791. 337

1

Akkermansia muciniphila SEQ ID NO: 136 202 67 348 418 62 348 418 62 ATCC BAA-835 338

gill87736489lreflYP_00187860

1.

Prevotella sp. C561 SEQ ID NO: 184 250 67 357 425 78 357 425 78 gil345885718lrellZP_08837074. 339

1

Wolinella succinogenes DSM SEQ ID NO: 157 218 36 401 468 60 401 468 60

1740 340

gil34557932lreflNP_907747.1l

Alicyclobacillus hesperidum SEQ ID NO: 142 196 55 416 482 61 416 482 61 U H17-3-68 341

gil403744858lreflZP_10953934.

1

Caenispirillum salinarum AK4 SEQ ID NO: 161 214 54 330 393 68 330 393 68 gil427429481 lreflZP_l 8919511. 342

1

Eubacterium rectale ATCC SEQ ID NO: 133 185 53 322 384 60 322 384 60 33656 343

gil238924075lrellYP_00293759

1.1

Mycoplasma synoviae 53 SEQ ID NO: 187 239 53 319 381 80 319 381 80 gil71894592lref 1 YP_278700.11 344

Porphyromonas sp. oral taxon SEQ ID NO: 150 202 53 309 371 60 309 371 60

279 str. F0450 345

gil402847315lrellZP_10895610.

1

Streptococcus thermophilus SEQ ID NO: 127 178 139 424 486 81 424 486 81 LMD-9 346

gil 116627542lrell YP_820161.11 oseburia inulinivorans DSM SEQ ID NO: 154 204 51 318 380 69 318 380 69 16841 347

gil225377804lreflZP_03755025.

1

Methylosinus trichosporium SEQ ID NO: 144 193 50 426 488 64 426 488 64 OB 3b 348

gil296446027lreflZP_06887976.

1

Ruminococcus albus 8 SEQ ID NO: 139 187 49 351 412 55 351 412 55 gil325677756lreflZP_08157403. 349

1

Bifidobacterium longum SEQ ID NO: 183 230 48 370 431 44 370 431 44 DJO10A 350

gill89440764lreflYP_00195584

5.

Enterococcus faecalis TX0012 SEQ ID NO: 123 170 48 327 387 60 327 387 60 gil315149830lgblEFT93846.11 351

Mycoplasma mobile 163K SEQ ID NO: 179 226 48 314 374 79 314 374 79 gil47458868lreflYP_015730.1l 352

Actinomyces coleocanis DSM SEQ ID NO: 147 193 47 358 418 40 358 418 40

15436 353

gil227494853lreflZP_03925169.

1

Dinoroseobacter shibae DFL 12 SEQ ID NO: 138 184 47 338 398 48 338 398 48 gil 159042956lrefl YP_00153175 354

0.1

Actinomyces sp. oral taxon 180 SEQ ID NO: 183 228 46 349 409 40 349 409 40 str. F0310 355

gil315605738lreflZP_07880770.

1

Alcanivorax sp. Wl l-5 SEQ ID NO: 139 183 45 344 404 61 344 404 61 gil407803669lreflZP_l 1150502. 356

1

Aminomonas paucivorans DSM SEQ ID NO: 134 178 45 341 401 63 341 401 63

12260 357

gil312879015lreflZP_07738815.

1

Mycoplasma canis PG 14 SEQ ID NO: 139 183 45 319 379 76 319 379 76 gil384393286lgblEIE39736.11 358

Lactobacillus coryniformis SEQ ID NO: 141 184 44 328 387 61 328 387 61 KCTC 3535 359

gil336393381lreflZP_08574780.

1

Elusimicrobium minutum SEQ ID NO: 177 219 43 322 381 47 322 381 47 Peil91 360

gill87250660lreflYP_00187514

2.1

Neisseria meningitidis Z2491 SEQ ID NO: 147 189 43 360 419 61 360 419 61 gil218767588lreflYP_00234210 361

0.1

Pasteurella multocida str. Pm70 SEQ ID NO: 139 181 43 319 378 61 319 378 61 gil 15602992lref INP_246064.11 362

Rhodovulum sp. PH10 SEQ ID NO: 141 183 43 319 378 48 319 378 48 gil402849997lreflZP_10898214. 363

1

Eubacterium dolichum DSM SEQ ID NO: 131 172 42 303 361 59 303 361 59

3991 364 gil 160915782lreflZP_02077990.

1

Nitratifractor salsuginis DSM SEQ ID NO: 143 184 42 347 404 61 347 404 61

16511 365

gil319957206lreflYP_00416846

9.1

Rhodospirillum rubrum ATCC SEQ ID NO: 139 180 42 314 371 55 314 371 55

11170 366

gil83591793lref 1 YP_425545.11

Clostridium cellulolyticum H10 SEQ ID NO: 137 176 40 320 376 61 320 376 61 gil220930482lreflYP_00250739 367

1.1

Helicobacter mustelae 12198 SEQ ID NO: 148 187 40 298 354 48 298 354 48 gil291276265lrellYP_00351603 368

7.1

Ilyobacter polytropus DSM SEQ ID NO: 134 173 40 462 517 63 462 517 63 2926 369

gil310780384lrellYP_00396871

6.1

Sphaerochaeta globus str. SEQ ID NO: 163 202 40 335 389 45 335 389 45 Buddy 370

gil325972003lrellYP_00424819

4.1

Staphylococcus lugdunensis SEQ ID NO: 128 167 40 337 391 57 337 391 57 M23590 371

gil315659848lrellZP_07912707.

1

Treponema sp. JC4 SEQ ID NO: 144 183 40 328 382 63 328 382 63 gil384109266lrellZP_10010146. 372

1

uncultured delta SEQ ID NO: 154 193 40 313 365 55 313 365 55 proteobacterium HF0070 373

07E19

gil297182908lgblADI19058.1 l

Alicycliphilus denitrificans SEQ ID NO: 140 178 39 317 366 48 317 366 48 K601 374

gil330822845lreflYP_00438614

8.1

Azospirillum sp. B510 SEQ ID NO: 205 243 39 342 389 46 342 389 46 gil288957741lreflYP_00344808 375

2.1

Bradyrhizobium sp. BTAil SEQ ID NO: 143 181 39 323 370 48 323 370 48 gill48255343lreflYP_00123992 376

8.1

Parvibaculum lavamentivorans SEQ ID NO: 138 176 39 327 374 58 327 374 58

DS-1 377

gill54250555lreflYP_00141137

9.1

Prevotella timonensis CRIS 5C- SEQ ID NO: 170 208 39 328 375 61 328 375 61

B l 378

gil282880052lreflZP_06288774.

1

Bacillus smithii 7 3 47FAA SEQ ID NO: 134 171 38 401 448 63 401 448 63 gil365156657lrellZP_09352959. 379

1

Cand. Puniceispirillum SEQ ID NO: 135 172 38 344 391 53 344 391 53 marinum IMCC1322 380

gil294086111lreflYP_00355287

1.1 Barnesiella intestinihominis SEQ ID NO: 140 176 37 371 417 60 371 417 60 YIT 11860 381

gil404487228lreflZP_l 1022414.

1

alstonia syzygii R24 SEQ ID NO: 140 176 37 395 440 50 395 440 50 gil344171927lemblCCA84553.1 382

1

Wolinella succinogenes DSM SEQ ID NO: 145 180 36 348 392 60 348 392 60

1740 383

gil34557790lref INP_907605.11

Mycoplasma gallisepticum str. SEQ ID NO: 144 177 34 373 416 71 373 416 71

F 384

gil284931710lgblADC31648.1l

Acidothermus cellulolyticus SEQ ID NO: 150 182 33 341 380 58 341 380 58

11B 385

gil 117929158lreflYP_873709.11

Mycoplasma ovipneumoniae SEQ ID NO: 156 184 29 381 420 62 381 420 62

SCOl 386

gil363542550lreflZP_09312133.

1

Table 18. Amino Acid Sequence of Cas9 Core Domains

Table 19. Identified PAM sequences and corresponding RKR motifs.

PI domains are provided in Tables 20 and 21. Table 20. Altered PI Domains

Table 21. Other Altered PI Domains

Actinomyces sp. oral taxon 180 str. F0310 895 1181 287 KEK

Treponema sp. JC4 832 1062 231 KIS

Fusobacteriumnucleatum ATCC49256 1073 1374 302 KKV

Lactobacillus farciminis KCTC 3681 1101 1356 256 KKV

Nitratifractor salsuginis DSM 16511 840 1132 293 KMR

Lactobacillus coryniformis KCTC 3535 850 1119 270 KNK

Mycoplasma mobile 163K 916 1236 321 KNY

Flavobacterium branchiophilum FL-15 1182 1473 292 KQK

Prevotella timonensis CRIS 5C-B1 957 1218 262 KQQ

Methylosinus trichosporium OB 3b 830 1082 253 KRP

Prevotella sp. C561 1099 1424 326 KRY

Mycoplasma gallisepticum str. F 911 1269 359 KTA

Lactobacillus rhamnosus GG 1077 1363 287 KYG

Wolinella succinogenes DSM 1740 811 1059 249 LPN

Streptococcus thermophilus LMD-9 1099 1388 290 MLA

Treponema denticola ATCC 35405 1092 1395 304 NDS

Bergeyella zoohelcum ATCC 43767 1098 1415 318 NEK

Veillonella atypica ACS-134-V-Col7a 1107 1398 292 NGF

Neisseria meningitidis Z2491 835 1082 248 NHN

Ignavibacterium album JCM 16511 1296 1688 393 NKK

Ruminococcus albus 8 853 1156 304 NNF

Streptococcus thermophilus LMD-9 811 1121 311 NNK

Barnesiella intestinihominis YIT 11860 871 1153 283 NPV

Azospirillum sp. B510 911 1168 258 PFH

Rhodospirillum rubrum ATCC 11170 863 1173 311 PRG

Planococcus antarcticus DSM 14505 1087 1333 247 PYY

Staphylococcus pseudintermedius ED99 1073 1334 262 QIV

Alcanivorax sp. Wll-5 843 1113 271 RIE

Bradyrhizobium sp. BTAil 811 1064 254 RIY

Streptococcus pyogenes M 1 GAS 1099 1368 270 RKR

Streptococcus mutans UA159 1078 1345 268 RKR

Streptococcus Pyogenes 1099 1368 270 RKR

Bacteroides sp. 20 3 1147 1517 371 RNI

S. aureus 772 1053 282 RNK

Solobacterium moorei F0204 1062 1327 266 RSG

Finegoldia magna ATCC 29328 1081 1348 268 RTE uncultured delta proteobacterium HF0070 07E19 770 1011 242 SGG

Acidaminococcus sp. D21 1064 1358 295 SIG

Eubacterium rectale ATCC 33656 824 1114 291 SKK

Caenispirillum salinarum AK4 1048 1442 395 SLV

Acidothermus cellulolyticus 1 IB 830 1138 309 SPS

Catenibacterium mitsuokai DSM 15897 1068 1329 262 SPT Parvibaculum lavamentivorans DS-1 827 1037 211 TGN

Staphylococcus lugdunensis M23590 772 1054 283 TKK

Streptococcus sanguinis SK49 1123 1421 299 TRM

Elusimicrobium minutum Peil91 910 1195 286 TTG

Nitrobacter hamburgensis X14 914 1166 253 VAY

Mycoplasma synoviae 53 991 1314 324 VGF

Sphaerochaeta globus str. Buddy 877 1179 303 VKG

Ilyobacter polytropus DSM 2926 837 1092 256 VNG

Rhodovulum sp. PH10 821 1059 239 VPY

Bifidobacterium longum DJO10A 904 1187 284 VRK

Amino acid sequences described in Table 17:

SEQ ID NO: 304

MKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRH RIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNVNEV EEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKV QKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVK YAYNADLYNALNDLNNLVITRDENEKLEYYEKFQI IENVFKQKKKPTLKQIAKEILVNEEDI KGYRVTSTGKPEFTNLKVYHDIKDITARKEI IENAELLDQIAKILTIYQSSEDIQEELTNLN SELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQK EIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQK RNRQTNERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHI IPRSVSFDNSFNNKVLVKQEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISK TKKEYLLEERDINRFSVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTS FLRRKWKFKKERNKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEI ETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLN GLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKY SKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKWKLSLKPYRFDVYLDNGVYKFVTVKN LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNRI EVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIKKG

SEQ ID NO: 305

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEAT RLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFI QLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGL TPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNT EITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEF YKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLK DNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT NFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIV LTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKW DELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQL QNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSD NVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAV VGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLAN GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNS DKLIARKKDWDPKKYGGFDSPTVAYSVLWAKVEKGKSKKLKSVKELLGITIMERSSFEKNP IDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI REQAENI IHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ LGGD

SEQ ID NO: 306

MARILAFDIGI SSIGWAFSENDELKDCGVRIFTKVENPKTGESLALPRRLARSARKRLARRK ARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYELRFRALNELLSKQDFARVILH IAKRRGYDDIKNSDDKEKGAILKAIKQNEEKLANYQSVGEYLYKEYFQKFKENSKEFTNVRN KKESYERCIAQSFLKDELKLIFKKQREFGFSFSKKFEEEVLSVAFYKRALKDFSHLVGNCSF FTDEKRAPKNSPLAFMFVALTRI INLLNNLKNTEGILYTKDDLNALLNEVLKNGTLTYKQTK KLLGLSDDYEFKGEKGTYFIEFKKYKEFIKALGEHNLSQDDLNEIAKDITLIKDEIKLKKAL AKYDLNQNQIDSLSKLEFKDHLNISFKALKLVTPLMLEGKKYDEACNELNLKVAINEDKKDF LPAFNETYYKDEVTNPWLRAIKEYRKVLNALLKKYGKVHKINIELAREVGKNHSQRAKIEK EQNENYKAKKDAELECEKLGLKINSKNILKLRLFKEQKEFCAYSGEKIKISDLQDEKMLEID HIYPYSRSFDDSYMNKVLVFTKQNQEKLNQTPFEAFGNDSAKWQKIEVLAKNLPTKKQKRIL DKNYKDKEQKNFKDRNLNDTRYIARLVLNYTKDYLDFLPLSDDENTKLNDTQKGSKVHVEAK SGMLTSALRHTWGFSAKDRNNHLHHAIDAVI IAYANNSIVKAFSDFKKEQESNSAELYAKKI SELDYKNKRKFFEPFSGFRQKVLDKIDEIFVSKPERKKPSGALHEETFRKEEEFYQSYGGKE GVLKALELGKIRKVNGKIVKNGDMFRVDIFKHKKTNKFYAVPIYTMDFALKVLPNKAVARSK KGEIKDWILMDENYEFCFSLYKDSLILIQTKDMQEPEFVYYNAFTSSTVSLIVSKHDNKFET LSKNQKILFKNANEKEVIAKSIGIQNLKVFEKYIVSALGEVTKAEFRQREDFKK

SEQ ID NO: 307

MKRILGLDLGTNSIGWALVNEAENKDERSSIVKLGVRVNPLTVDELTNFEKGKSITTNADRT LKRGMRRNLQRYKLRRETLTEVLKEHKLITEDTILSENGNRTTFETYRLRAKAVTEEISLEE FARVLLMINKKRGYKSSRKAKGVEEGTLIDGMDIARELYNNNLTPGELCLQLLDAGKKFLPD FYRSDLQNELDRIWEKQKEYYPEILTDVLKEELRGKKRDAVWAICAKYFVWKENYTEWNKEK GKTEQQEREHKLEGIYSKRKRDEAKRENLQWRVNGLKEKLSLEQLVIVFQEMNTQINNSSGY LGAISDRSKELYFNKQTVGQYQMEMLDKNPNASLRNMVFYRQDYLDEFNMLWEKQAVYHKEL TEELKKEIRDI IIFYQRRLKSQKGLIGFCEFESRQIEVDIDGKKKIKTVGNRVISRSSPLFQ EFKIWQILNNIEVTVVGKKRKRRKLKENYSALFEELNDAEQLELNGSRRLCQEEKELLAQEL FIRDKMTKSEVLKLLFDNPQELDLNFKTIDGNKTGYALFQAYSKMIEMSGHEPVDFKKPVEK WEYIKAVFDLLNWNTDILGFNSNEELDNQPYYKLWHLLYSFEGDNTPTGNGRLIQKMTELY GFEKEYATILANVSFQDDYGSLSAKAIHKILPHLKEGNRYDVACVYAGYRHSESSLTREEIA NKVLKDRLMLLPKNSLHNPWEKILNQMVNVINVI IDIYGKPDEIRVELARELKKNAKEREE LTKSIAQTTKAHEEYKTLLQTEFGLTNVSRTDILRYKLYKELESCGYKTLYSNTYISREKLF SKEFDIEHIIPQARLFDDSFSNKTLEARSVNIEKGNKTAYDFVKEKFGESGADNSLEHYLNN IEDLFKSGKISKTKYNKLKMAEQDIPDGFIERDLRNTQYIAKKALSMLNEISHRVVATSGSV TDKLREDWQLIDVMKELNWEKYKALGLVEYFEDRDGRQIGRIKDWTKRNDHRHHAMDALTVA FTKDVFIQYFNNKNASLDPNANEHAIKNKYFQNGRAIAPMPLREFRAEAKKHLENTLISIKA KNKVITGNINKTRKKGGVNKNMQQTPRGQLHLETIYGSGKQYLTKEEKVNASFDMRKIGTVS KSAYRDALLKRLYENDNDPKKAFAGKNSLDKQPIWLDKEQMRKVPEKVKIVTLEAIYTIRKE ISPDLKVDKVIDVGVRKILIDRLNEYGNDAKKAFSNLDKNPIWLNKEKGISIKRVTISGISN AQSLHVKKDKDGKPILDENGRNIPVDFVNTGNNHHVAVYYRPVIDKRGQLVVDEAGNPKYEL EEVWSFFEAVTRANLGLPIIDKDYKTTEGWQFLFSMKQNEYFVFPNEKTGFNPKEIDLLDV ENYGLISPNLFRVQKFSLKNYVFRHHLETTIKDTSSILRGITWIDFRSSKGLDTIVKVRVNH IGQIVSVGEY SEQ ID NO: 308

MSRKNYVDDYAISLDIGNASVGWSAFTPNYRLVRAKGHELIGVRLFDPADTAESRRMARTTR RRYSRRRWRLRLLDALFDQALSEIDPSFLARRKYSWVHPDDENNADCWYGSVLFDSNEQDKR FYEKYPTIYHLRKALMEDDSQHDIREIYLAIHHMVKYRGNFLVEGTLESSNAFKEDELLKLL GRITRYEMSEGEQNSDIEQDDENKLVAPANGQLADALCATRGSRSMRVDNALEALSAVNDLS REQRAIVKAIFAGLEGNKLDLAKIFVSKEFSSENKKILGIYFNKSDYEEKCVQIVDSGLLDD EEREFLDRMQGQYNAIALKQLLGRSTSVSDSKCASYDAHRANWNLIKLQLRTKENEKDINEN YGILVGWKIDSGQRKSVRGESAYENMRKKANVFFKKMIETSDLSETDKNRLIHDIEEDKLFP IQRDSDNGVIPHQLHQNELKQIIKKQGKYYPFLLDAFEKDGKQINKIEGLLTFRVPYFVGPL WPEDLQKSDNSENHWMVRKKKGEITPWNFDEMVDKDASGRKFIERLVGTDSYLLGEPTLPK NSLLYQEYEVLNELNNVRLSVRTGNHWNDKRRMRLGREEKTLLCQRLFMKGQTVTKRTAENL LRKEYGRTYELSGLSDESKFTSSLSTYGKMCRIFGEKYVNEHRDLMEKIVELQTVFEDKETL LHQLRQLEGISEADCALLVNTHYTGWGRLSRKLLTTKAGECKISDDFAPRKHSIIEIMRAED RNLMEIITDKQLGFSDWIEQENLGAENGSSLMEWDDLRVSPKVKRGIIQSIRLIDDISKAV GKRPSRIFLELADDIQPSGRTISRKSRLQDLYRNANLGKEFKGIADELNACSDKDLQDDRLF LYYTQLGKDMYTGEELDLDRLSSAYDIDHIIPQAVTQNDSIDNRVLVARAENARKTDSFTYM PQIADRMRNFWQILLDNGLISRVKFERLTRQNEFSEREKERFVQRSLVETRQIMKNVATLMR QRYGNSAAVIGLNAELTKEMHRYLGFSHKNRDINDYHHAQDALCVGIAGQFAANRGFFADGE VSDGAQNSYNQYLRDYLRGYREKLSAEDRKQGRAFGFIVGSMRSQDEQKRVNPRTGEWWSE EDKDYLRKVMNYRKMLVTQKVGDDFGALYDETRYAATDPKGIKGIPFDGAKQDTSLYGGFSS AKPAYAVLIESKGKTRLVNVTMQEYSLLGDRPSDDELRKVLAKKKSEYAKANILLRHVPKMQ LIRYGGGLMVIKSAGELNNAQQLWLPYEEYCYFDDLSQGKGSLEKDDLKKLLDSILGSVQCL YPWHRFTEEELADLHVAFDKLPEDEKKNVITGIVSALHADAKTANLSIVGMTGSWRRMNNKS GYTFSDEDEFIFQSPSGLFEKRVTVGELKRKAKKEVNSKYRTNEKRLPTLSGASQP

SEQ ID NO: 309

METQTSNQLITSHLKDYPKQDYFVGLDIGTNSVGWAVTNTSYELLKFHSHKMWGSRLFEEGE SAVTRRGFRSMRRRLERRKLRLKLLEELFADAMAQVDSTFFIRLHESKYHYEDKTTGHSSKH ILFIDEDYTDQDYFTEYPTIYHLRKDLMENGTDDIRKLFLAVHHILKYRGNFLYEGATFNSN AFTFEDVLKQALVNITFNCFDTNSAISSISNILMESGKTKSDKAKAIERLVDTYTVFDEVNT PDKPQKEQVKEDKKTLKAFANLVLGLSANLIDLFGSVEDIDDDLKKLQIVGDTYDEKRDELA KVWGDEIHIIDDCKSVYDAIILMSIKEPGLTISQSKVKAFDKHKEDLVILKSLLKLDRNVYN EMFKSDKKGLHNYVHYIKQGRTEETSCSREDFYKYTKKIVEGLADSKDKEYILNEIELQTLL PLQRIKDNGVIPYQLHLEELKVILDKCGPKFPFLHTVSDGFSVTEKLIKMLEFRIPYYVGPL NTHHNIDNGGFSWAVRKQAGRVTPWNFEEKIDREKSAAAFIKNLTNKCTYLFGEDVLPKSSL LYSEFMLLNELNNVRIDGKALAQGVKQHLIDSIFKQDHKKMTKNRIELFLKDNNYITKKHKP EITGLDGEIKNDLTSYRDMVRILGNNFDVSMAEDI ITDITIFGESKKMLRQTLRNKFGSQLN DETIKKLSKLRYRDWGRLSKKLLKGIDGCDKAGNGAPKTIIELMRNDSYNLMEILGDKFSFM ECIEEENAKLAQGQVVNPHDIIDELALSPAVKRAVWQALRIVDEVAHIKKALPSRIFVEVAR TNKSEKKKKDSRQKRLSDLYSAIKKDDVLQSGLQDKEFGALKSGLANYDDAALRSKKLYLYY TQMGRCAYTGNIIDLNQLNTDNYDIDHIYPRSLTKDDSFDNLVLCERTANAKKSDIYPIDNR IQTKQKPFWAFLKHQGLISERKYERLTRIAPLTADDLSGFIARQLVETNQSVKATTTLLRRL YPDIDWFVKAENVSDFRHNNNFIKVRSLNHHHHAKDAYLNIVVGNVYHEKFTRNFRLFFKK NGANRTYNLAKMFNYDVICTNAQDGKAWDVKTSMNTVKKMMASNDVRVTRRLLEQSGALADA TIYKASVAAKAKDGAYIGMKTKYSVFADVTKYGGMTKIKNAYSIIVQYTGKKGEEIKEIVPL PIYLINRNATDIELIDYVKSVIPKAKDISIKYRKLCINQLVKVNGFYYYLGGKTNDKIYIDN AIELWPHDIATYIKLLDKYDLLRKENKTLKASSITTSIYNINTSTVVSLNKVGIDVFDYFM SKLRTPLYMKMKGNKVDELSSTGRSKFIKMTLEEQSIYLLEVLNLLTNSKTTFDVKPLGITG SRSTIGVKIHNLDEFKIINESITGLYSNEVTIV SEQ ID NO: 310

MTKLNQPYGIGLDIGSNSIGFAVVDANSHLLRLKGETAIGARLFREGQSAADRRGSRTTRRR LSRTRWRLSFLRDFFAPHITKIDPDFFLRQKYSEISPKDKDRFKYEKRLFNDRTDAEFYEDY PSMYHLRLHLMTHTHKADPREIFLAIHHILKSRGHFLTPGAAKDFNTDKVDLEDIFPALTEA YAQVYPDLELTFDLAKADDFKAKLLDEQATPSDTQKALVNLLLSSDGEKEIVKKRKQVLTEF AKAITGLKTKFNLALGTEVDEADASNWQFSMGQLDDKWSNIETSMTDQGTEIFEQIQELYRA RLLNGIVPAGMSLSQAKVADYGQHKEDLELFKTYLKKLNDHELAKTIRGLYDRYINGDDAKP FLREDFVKALTKEVTAHPNEVSEQLLNRMGQANFMLKQRTKANGAIPIQLQQRELDQIIANQ SKYYDWLAAPNPVEAHRWKMPYQLDELLNFHIPYYVGPLITPKQQAESGENVFAWMVRKDPS GNITPYNFDEKVDREASANTFIQRMKTTDTYLIGEDVLPKQSLLYQKYEVLNELNNVRINNE CLGTDQKQRLIREVFERHSSVTIKQVADNLVAHGDFARRPEIRGLADEKRFLSSLSTYHQLK EILHEAIDDPTKLLDIENI ITWSTVFEDHTIFETKLAEIEWLDPKKINELSGIRYRGWGQFS RKLLDGLKLGNGHTVIQELMLSNHNLMQILADETLKETMTELNQDKLKTDDIEDVINDAYTS PSNKKALRQVLRWEDIKHAANGQDPSWLFIETADGTGTAGKRTQSRQKQIQTVYANAAQEL IDSAVRGELEDKIADKASFTDRLVLYFMQGGRDIYTGAPLNIDQLSHYDIDHILPQSLIKDD SLDNRVLVNATINREKNNVFASTLFAGKMKATWRKWHEAGLISGRKLRNLMLRPDEIDKFAK GFVARQLVETRQIIKLTEQIAAAQYPNTKIIAVKAGLSHQLREELDFPKNRDVNHYHHAFDA FLAARIGTYLLKRYPKLAPFFTYGEFAKVDVKKFREFNFIGALTHAKKNIIAKDTGEIVWDK ERDIRELDRIYNFKRMLITHEVYFETADLFKQTIYAAKDSKERGGSKQLIPKKQGYPTQVYG GYTQESGSYNALVRVAEADTTAYQVIKISAQNASKIASANLKSREKGKQLLNEIVVKQLAKR RKNWKPSANSFKIVIPRFGMGTLFQNAKYGLFMVNSDTYYRNYQELWLSRENQKLLKKLFSI KYEKTQMNHDALQVYKAIIDQVEKFFKLYDINQFRAKLSDAIERFEKLPINTDGNKIGKTET LRQILIGLQANGTRSNVKNLGIKTDLGLLQVGSGIKLDKDTQIVYQSPSGLFKRRIPLADL SEQ ID NO: 311

MTKEYYLGLDVGTNSVGWAVTDSQYNLCKFKKKDMWGIRLFESANTAKDRRLQRGNRRRLER KKQRIDLLQEIFSPEICKIDPTFFIRLNESRLHLEDKSNDFKYPLFIEKDYSDIEYYKEFPT IFHLRKHLIESEEKQDIRLIYLALHNI IKTRGHFLIDGDLQSAKQLRPILDTFLLSLQEEQN LSVSLSENQKDEYEEILKNRSIAKSEKVKKLKNLFEISDELEKEEKKAQSAVIENFCKFIVG NKGDVCKFLRVSKEELEIDSFSFSEGKYEDDIVKNLEEKVPEKVYLFEQMKAMYDWNILVDI LETEEYISFAKVKQYEKHKTNLRLLRDIILKYCTKDEYNRMFNDEKEAGSYTAYVGKLKKNN KKYWIEKKRNPEEFYKSLGKLLDKIEPLKEDLEVLTMMIEECKNHTLLPIQKNKDNGVIPHQ VHEVELKKILENAKKYYSFLTETDKDGYSWQKIESIFRFRIPYYVGPLSTRHQEKGSNVWM VRKPGREDRIYPWNMEEIIDFEKSNENFITRMTNKCTYLIGEDVLPKHSLLYSKYMVLNELN NVKVRGKKLPTSLKQKVFEDLFENKSKVTGKNLLEYLQIQDKDIQIDDLSGFDKDFKTSLKS YLDFKKQIFGEEIEKESIQNMIEDIIKWITIYGNDKEMLKRVIRANYSNQLTEEQMKKITGF QYSGWGNFSKMFLKGISGSDVSTGETFDIITAMWETDNNLMQILSKKFTFMDNVEDFNSGKV GKIDKITYDSTVKEMFLSPENKRAVWQTIQVAEEIKKVMGCEPKKIFIEMARGGEKVKKRTK SRKAQLLELYAACEEDCRELIKEIEDRDERDFNSMKLFLYYTQFGKCMYSGDDIDINELIRG NSKWDRDHIYPQSKIKDDSIDNLVLVNKTYNAKKSNELLSEDIQKKMHSFWLSLLNKKLITK SKYDRLTRKGDFTDEELSGFIARQLVETRQSTKAIADIFKQIYSSEVVYVKSSLVSDFRKKP LNYLKSRRVNDYHHAKDAYLNIVVGNVYNKKFTSNPIQWMKKNRDTNYSLNKVFEHDWING EVIWEKCTYHEDTNTYDGGTLDRIRKIVERDNILYTEYAYCEKGELFNATIQNKNGNSTVSL KKGLDVKKYGGYFSANTSYFSLIEFEDKKGDRARHIIGVPIYIANMLEHSPSAFLEYCEQKG YQNVRILVEKIKKNSLLIINGYPLRIRGENEVDTSFKRAIQLKLDQKNYELVRNIEKFLEKY VEKKGNYPIDENRDHITHEKMNQLYEVLLSKMKKFNKKGMADPSDRIEKSKPKFIKLEDLID KINVINKMLNLLRCDNDTKADLSLIELPKNAGSFVVKKNTIGKSKIILVNQSVTGLYENRRE L SEQ ID NO: 312 MARDYSVGLDIGTSSVGWAAIDNKYHLIRAKSKNLIGVRLFDSAVTAEKRRGYRTTRRRLSR RHWRLRLLNDIFAGPLTDFGDENFLARLKYSWVHPQDQSNQAHFAAGLLFDSKEQDKDFYRK YPTIYHLRLALMNDDQKHDLREVYLAIHHLVKYRGHFLIEGDVKADSAFDVHTFADAIQRYA ESNNSDENLLGKIDEKKLSAALTDKHGSKSQRAETAETAFDILDLQSKKQIQAILKSWGNQ ANLMAIFGLDSSAISKDEQKNYKFSFDDADIDEKIADSEALLSDTEFEFLCDLKAAFDGLTL KMLLGDDKTVSAAMVRRFNEHQKDWEYIKSHIRNAKNAGNGLYEKSKKFDGINAAYLALQSD NEDDRKKAKKIFQDEISSADIPDDVKADFLKKIDDDQFLPIQRTKNNGTIPHQLHRNELEQI IEKQGIYYPFLKDTYQENSHELNKITALINFRVPYYVGPLVEEEQKIADDGKNIPDPTNHWM VRKSNDTITPWNLSQWDLDKSGRRFIERLTGTDTYLIGEPTLPKNSLLYQKFDVLQELNNI RVSGRRLDIRAKQDAFEHLFKVQKTVSATNLKDFLVQAGYISEDTQIEGLADVNGKNFNNAL TTYNYLVSVLGREFVENPSNEELLEEITELQTVFEDKKVLRRQLDQLDGLSDHNREKLSRKH YTGWGRISKKLLTTKIVQNADKIDNQTFDVPRMNQSIIDTLYNTKMNLMEI INNAEDDFGVR AWIDKQNTTDGDEQDVYSLIDELAGPKEIKRGIVQSFRILDDITKAVGYAPKRVYLEFARKT QESHLTNSRKNQLSTLLKNAGLSELVTQVSQYDAAALQNDRLYLYFLQQGKDMYSGEKLNLD NLSNYDIDHIIPQAYTKDNSLDNRVLVSNITNRRKSDSSNYLPALIDKMRPFWSVLSKQGLL SKHKFANLTRTRDFDDMEKERFIARSLVETRQIIKNVASLIDSHFGGETKAVAIRSSLTADM RRYVDIPKNRDINDYHHAFDALLFSTVGQYTENSGLMKKGQLSDSAGNQYNRYIKEWIHAAR LNAQSQRVNPFGFWGSMRNAAPGKLNPETGEITPEENADWSIADLDYLHKVMNFRKITVTR RLKDQKGQLYDESRYPSVLHDAKSKASINFDKHKPVDLYGGFSSAKPAYAALIKFKNKFRLV NVLRQWTYSDKNSEDYILEQIRGKYPKAEMVLSHIPYGQLVKKDGALVTISSATELHNFEQL WLPLADYKLINTLLKTKEDNLVDILHNRLDLPEMTIESAFYKAFDSILSFAFNRYALHQNAL VKLQAHRDDFNALNYEDKQQTLERILDALHASPASSDLKKINLSSGFGRLFSPSHFTLADTD EFIFQSVTGLFSTQKTVAQLYQETK SEQ ID NO: 313

MVYDVGLDIGTGSVGWVALDENGKLARAKGKNLVGVRLFDTAQTAADRRGFRTTRRRLSRRK WRLRLLDELFSAEINEIDSSFFQRLKYSYVHPKDEENKAHYYGGYLFPTEEETKKFHRSYPT IYHLRQELMAQPNKRFDIREIYLAIHHLVKYRGHFLSSQEKITIGSTYNPEDLANAIEVYAD EKGLSWELNNPEQLTEIISGEAGYGLNKSMKADEALKLFEFDNNQDKVAIKTLLAGLTGNQI DFAKLFGKDISDKDEAKLWKLKLDDEALEEKSQTILSQLTDEEIELFHAWQAYDGFVLIGL LNGADSVSAAMVQLYDQHREDRKLLKSLAQKAGLKHKRFSEIYEQLALATDEATIKNGISTA RELVEESNLSKEVKEDTLRRLDENEFLPKQRTKANSVIPHQLHLAELQKILQNQGQYYPFLL DTFEKEDGQDNKIEELLRFRIPYYVGPLVTKKDVEHAGGDADNHWVERNEGFEKSRVTPWNF DKVFNRDKAARDFIERLTGNDTYLIGEKTLPQNSLRYQLFTVLNELNNVRVNGKKFDSKTKA DLINDLFKARKTVSLSALKDYLKAQGKGDVTITGLADESKFNSSLSSYNDLKKTFDAEYLEN EDNQETLEKIIEIQTVFEDSKIASRELSKLPLDDDQVKKLSQTHYTGWGRLSEKLLDSKIID ERGQKVSILDKLKSTSQNFMSIINNDKYGVQAWITEQNTGSSKLTFDEKVNELTTSPANKRG IKQSFAVLNDIKKAMKEEPRRVYLEFAREDQTSVRSVPRYNQLKEKYQSKSLSEEAKVLKKT LDGNKNKMSDDRYFLYFQQQGKDMYTGRPINFERLSQDYDIDHIIPQAFTKDDSLDNRVLVS RPENARKSDSFAYTDEVQKQDGSLWTSLLKSGFINRKKYERLTKAGKYLDGQKTGFIARQLV ETRQ11KNVASLIEGEYENSKAVAIRSEITADMRLLVGIKKHREINSFHHAFDALLITAAGQ YMQNRYPDRDSTNVYNEFDRYTNDYLKNLRQLSSRDEVRRLKSFGFVVGTMRKGNEDWSEEN TSYLRKVMMFKNILTTKKTEKDRGPLNKETIFSPKSGKKLIPLNSKRSDTALYGGYSNVYSA YMTLVRANGKNLLIKIPISIANQIEVGNLKINDYIVNNPAIKKFEKILISKLPLGQLVNEDG NLIYLASNEYRHNAKQLWLSTTDADKIASISENSSDEELLEAYDILTSENVKNRFPFFKKDI DKLSQVRDEFLDSDKRIAVIQTILRGLQIDAAYQAPVKI ISKKVSDWHKLQQSGGIKLSDNS EMIYQSATGIFETRVKISDLL

SEQ ID NO: 314

IVDYCIGLDLGTGSVGWAVVDMNHRLMKRNGKHLWGSRLFSNAETAANRRASRSIRRRYNKR RERIRLLRAILQDMVLEKDPTFFIRLEHTSFLDEEDKAKYLGTDYKDNYNLFIDEDFNDYTY YHKYPTIYHLRKALCESTEKADPRLIYLALHHIVKYRGNFLYEGQKFNMDASNIEDKLSDIF TQFTSFNNIPYEDDEKKNLEILEILKKPLSKKAKVDEVMTLIAPEKDYKSAFKELVTGIAGN KMNVTKMILCEPIKQGDSEIKLKFSDSNYDDQFSEVEKDLGEYVEFVDALHNVYSWVELQTI MGATHTDNASISEAMVSRYNKHHDDLKLLKDCIKNNVPNKYFDMFRNDSEKSKGYYNYINRP SKAPVDEFYKYVKKCIEKVDTPEAKQILNDIELENFLLKQNSRTNGSVPYQMQLDEMIKIID NQAEYYPILKEKREQLLSILTFRIPYYFGPLNETSEHAWIKRLEGKENQRILPWNYQDIVDV DATAEGFIKRMRSYCTYFPDEEVLPKNSLIVSKYEVYNELNKIRVDDKLLEVDVKNDIYNEL FMKNKTVTEKKLKNWLVNNQCCSKDAEIKGFQKENQFSTSLTPWIDFTNIFGKIDQSNFDLI E I IYDLTVFEDKKIMKRRLKKKYALPDDKVKQILKLKYKDWSRLSKKLLDGIVADNRFGSS VTVLDVLEMSRLNLMEIINDKDLGYAQMIEEATSCPEDGKFTYEEVERLAGSPALKRGIWQS LQIVEEITKVMKCRPKYIYIEFERSEEAKERTESKIKKLENVYKDLDEQTKKEYKSVLEELK GFDNTKKISSDSLFLYFTQLGKCMYSGKKLDIDSLDKYQIDHIVPQSLVKDDSFDNRVLWP SENQRKLDDLVVPFDIRDKMYRFWKLLFDHELISPKKFYSLIKTEYTERDEERFINRQLVET RQITKNVTQIIEDHYSTTKVAAIRANLSHEFRVKNHIYKNRDINDYHHAHDAYIVALIGGFM RDRYPNMHDSKAVYSEYMKMFRKNKNDQKRWKDGFVINSMNYPYEVDGKLIWNPDLINEIKK CFYYKDCYCTTKLDQKSGQLFNLTVLSNDAHADKGVTKAWPVNKNRSDVHKYGGFSGLQYT IVAIEGQKKKGKKTELVKKISGVPLHLKAASINEKINYIEEKEGLSDVRIIKDNIPVNQMIE MDGGEYLLTSPTEYVNARQLVLNEKQCALIADIYNAIYKQDYDNLDDILMIQLYIELTNKMK VLYPAYRGIAEKFESMNENYWISKEEKA I IKQMLIVMHRGPQNG IVYDDFKISDRIGRL KTKNHNLNNIVFISQSPTGIYTKKYKL

SEQ ID NO: 315

MKSEKKYYIGLDVGTNSVGWAVTDEFYNILRAKGKDLWGVRLFEKADTAANTRIFRSGRRRN DRKGMRLQILREIFEDEIKKVDKDFYDRLDESKFWAEDKKVSGKYSLFNDKNFSDKQYFEKF PTIFHLRKYLMEEHGKVDIRYYFLAINQMMKRRGHFLIDGQISHVTDDKPLKEQLILLINDL LKIELEEELMDSIFEILADVNEKRTDKKNNLKELIKGQDFNKQEGNILNSIFESIVTGKAKI KNI ISDEDILEKIKEDNKEDFVLTGDSYEENLQYFEEVLQENITLFNTLKSTYDFLILQSIL KGKSTLSDAQVERYDEHKKDLEILKKVIKKYDEDGKLFKQVFKEDNGNGYVSYIGYYLNKNK KITAKKKISNIEFTKYVKGILEKQCDCEDEDVKYLLGKIEQENFLLKQISSINSVIPHQIHL FELDKILENLAKNYPSFNNKKEEFTKIEKIRKTFTFRIPYYVGPLNDYHKNNGGNAWIFRNK GEKIRPWNFEKIVDLHKSEEEFIKRMLNQCTYLPEETVLPKSSILYSEYMVLNELNNLRING KPLDTDVKLKLIEELFKKKTKVTLKSIRDYMVRNNFADKEDFDNSEKNLEIASNMKSYIDFN NILEDKFDVEMVEDLIEKITIHTGNKKLLKKYIEETYPDLSSSQIQKIINLKYKDWGRLSRK LLDGIKGTKKETEKTDTVINFLRNSSDNLMQIIGSQNYSFNEYIDKLRKKYIPQEISYEWE NLYVSPSVKKMIWQVIRVTEEITKVMGYDPDKIFIEMAKSEEEKKTTISRKNKLLDLYKAIK KDERDSQYEKLLTGLNKLDDSDLRSRKLYLYYTQMGRDMYTGEKIDLDKLFDSTHYDKDHII PQSMKKDDSIINNLVLVNKNANQTTKGNIYPVPSSIRNNPKIYNYWKYLMEKEFISKEKYNR LIRNTPLTNEELGGFINRQLVETRQSTKAIKELFEKFYQKSKI IPVKASLASDLRKDMNTLK SREVNDLHHAHDAFLNIVAGDVWNREFTSNPINYVKENREGDKVKYSLSKDFTRPRKSKGKV IWTPEKGRKLIVDTLNKPSVLISNESHVKKGELFNATIAGKKDYKKGKIYLPLKKDDRLQDV SKYGGYKAINGAFFFLVEHTKSKKRIRSIELFPLHLLSKFYEDKNTVLDYAINVLQLQDPKI IIDKINYRTEI IIDNFSYLISTKSNDGSITVKPNEQMYWRVDEISNLKKIENKYKKDAILTE EDRKIMESYIDKIYQQFKAGKYKNRRTTDTI IEKYEIIDLDTLDNKQLYQLLVAFISLSYKT SNNAVDFTVIGLGTECGKPRITNLPDNTYLVYKSITGIYEKRIRIK

SEQ ID NO: 316

MKLRGIEDDYSIGLDMGTSSVGWAVTDERGTLAHFKRKPTWGSRLFREAQTAAVARMPRGQR RRYVRRRWRLDLLQKLFEQQMEQADPDFFIRLRQSRLLRDDRAEEHADYRWPLFNDCKFTER DYYQRFPTIYHVRSWLMETDEQADIRLIYLALHNIVKHRGNFLREGQSLSAKSARPDEALNH LRETLRVWSSERGFECSIADNGSILAMLTHPDLSPSDRRKKIAPLFDVKSDDAAADKKLGIA LAGAVIGLKTEFKNIFGDFPCEDSSIYLSNDEAVDAVRSACPDDCAELFDRLCEVYSAYVLQ GLLSYAPGQTISANMVEKYRRYGEDLALLKKLVKIYAPDQYRMFFSGATYPGTGIYDAAQAR GYTKYNLGPKKSEYKPSESMQYDDFRKAVEKLFAKTDARADERYRMMMDRFDKQQFLRRLKT SDNGSIYHQLHLEELKAIVENQGRFYPFLKRDADKLVSLVSFRIPYYVGPLSTRNARTDQHG ENRFAWSERKPGMQDEPIFPWNWESIIDRSKSAEKFILRMTGMCTYLQQEPVLPKSSLLYEE FCVLNELNGAHWSIDGDDEHRFDAADREGIIEELFRRKRTVSYGDVAGWMERERNQIGAHVC GGQGEKGFESKLGSYIFFCKDVFKVERLEQSDYPMIERI ILWNTLFEDRKILSQRLKEEYGS RLSAEQIKTICKKRFTGWGRLSEKFLTGITVQVDEDSVSIMDVLREGCPVSGKRGRAMVMME ILRDEELGFQKKVDDFNRAFFAENAQALGVNELPGSPAVRRSLNQSIRIVDEIASIAGKAPA NIFIEVTRDEDPKKKGRRTKRRYNDLKDALEAFKKEDPELWRELCETAPNDMDERLSLYFMQ RGKCLYSGRAIDIHQLSNAGIYEVDHI IPRTYVKDDSLENKALVYREENQRKTDMLLIDPEI RRRMSGYWRMLHEAKLIGDKKFRNLLRSRIDDKALKGFIARQLVETGQMVKLVRSLLEARYP ETNIISVKASISHDLRTAAELVKCREANDFHHAHDAFLACRVGLFIQKRHPCVYENPIGLSQ WRNYVRQQADIFKRCRTIPGSSGFIVNSFMTSGFDKETGEIFKDDWDAEAEVEGIRRSLNF RQCFISRMPFEDHGVFWDATIYSPRAKKTAALPLKQGLNPSRYGSFSREQFAYFFIYKARNP RKEQTLFEFAQVPVRLSAQIRQDENALERYARELAKDQGLEFIRIERSKILKNQLIEIDGDR LCITGKEEVRNACELAFAQDEMRVIRMLVSEKPVSRECVISLFNRILLHGDQASRRLSKQLK LALLSEAFSEASDNVQRNVVLGLIAIFNGSTNMVNLSDIGGSKFAGNVRIKYKKELASPKVN VHLIDQSVTGMFERRTKIGL SEQ ID NO: 317

MENKQYYIGLDVGTNSVGWAVTDTSYNLLRAKGKDMWGARLFEKANTAAERRTKRTSRRRSE REKARKAMLKELFADEINRVDPSFFIRLEESKFFLDDRSENNRQRYTLFNDATFTDKDYYEK YKTIFHLRSALINSDEKFDVRLVFLAILNLFSHRGHFLNASLKGDGDIQGMDVFYNDLVESC EYFEIELPRITNIDNFEKILSQKGKSRTKILEELSEELSISKKDKSKYNLIKLISGLEASW ELYNIEDIQDENKKIKIGFRESDYEESSLKVKEIIGDEYFDLVERAKSVHDMGLLSNIIGNS KYLCEARVEAYENHHKDLLKIKELLKKYDKKAYNDMFRKMTDKNYSAYVGSVNSNIAKERRS VDKRKIEDLYKYIEDTALKNIPDDNKDKIEILEKIKLGEFLKKQLTASNGVIPNQLQSRELR AILKKAENYLPFLKEKGEKNLTVSEMI IQLFEFQIPYYVGPLDKNPKKDNKANSWAKIKQGG RILPWNFEDKVDVKGSRKEFIEKMVRKCTYISDEHTLPKQSLLYEKFMVLNEINNIKIDGEK ISVEAKQKIYNDLFVKGKKVSQKDIKKELISLNIMDKDSVLSGTDTVCNAYLSSIGKFTGVF KEEINKQSIVDMIEDIIFLKTVYGDEKRFVKEEIVEKYGDEIDKDKIKRILGFKFSNWGNLS KSFLELEGADVGTGEVRSI IQSLWETNFNLMELLSSRFTYMDELEKRVKKLEKPLSEWTIED LDDMYLSSPVKRMIWQSMKIVDEIQTVIGYAPKRIFVEMTRSEGEKVRTKSRKDRLKELYNG IKEDSKQWVKELDSKDESYFRSKKMYLYYLQKGRCMYSGEVIELDKLMDDNLYDIDHIYPRS FVKDDSLDNLVLVKKEINNRKQNDPITPQIQASCQGFWKILHDQGFMSNEKYSRLTRKTQEF SDEEKLSFINRQIVETGQATKCMAQILQKSMGEDVDWFSKARLVSEFRHKFELFKSRLIND FHHANDAYLNIWGNSYFVKFTRNPANFIKDARKNPDNPVYKYHMDRFFERDVKSKSEVAWI GQSEGNSGTIVIVKKTMAKNSPLITKKVEEGHGSITKETIVGVKEIKFGRNKVEKADKTPKK PNLQAYRPIKTSDERLCNILRYGGRTSISISGYCLVEYVKKRKTIRSLEAIPVYLGRKDSLS EEKLLNYFRYNLNDGGKDSVSDIRLCLPFISTNSLVKIDGYLYYLGGKNDDRIQLYNAYQLK MKKEEVEYIRKIEKAVSMSKFDEIDREKNPVLTEEKNIELYNKIQDKFENTVFSKRMSLVKY NKKDLSFGDFLKNKKSKFEEIDLEKQCKVLYNIIFNLSNLKEVDLSDIGGSKSTGKCRCKKN ITNYKEFKLIQQSITGLYSCEKDLMTI SEQ ID NO: 318

MKNLKEYYIGLDIGTASVGWAVTDESYNIPKFNGKKMWGVRLFDDAKTAEERRTQRGSRRRL NRRKERINLLQDLFATEISKVDPNFFLRLDNSDLYREDKDEKLKSKYTLFNDKDFKDRDYHK KYPTIHHLIMDLIEDEGKKDIRLLYLACHYLLKNRGHFIFEGQKFDTKNSFDKSINDLKIHL RDEYNIDLEFNNEDLIEIITDTTLNKTNKKKELKNIVGDTKFLKAISAIMIGSSQKLVDLFE DGEFEETTVKSVDFSTTAFDDKYSEYEEALGDTISLLNILKSIYDSSILENLLKDADKSKDG NKYISKAFVKKFNKHGKDLKTLKRIIKKYLPSEYANIFRNKSINDNYVAYTKSNITSNKRTK ASKFTKQEDFYKFIKKHLDTIKETKLNSSENEDLKLIDEMLTDIEFKTFIPKLKSSDNGVIP YQLKLMELKKILDNQSKYYDFLNESDEYGTVKDKVESIMEFRIPYYVGPLNPDSKYAWIKRE NTKITPWNFKDIVDLDSSREEFIDRLIGRCTYLKEEKVLPKASLIYNEFMVLNELNNLKLNE FLITEEMKKAIFEELFKTKKKVTLKAVSNLLKKEFNLTGDILLSGTDGDFKQGLNSYIDFKN IIGDKVDRDDYRIKIEEIIKLIVLYEDDKTYLKKKIKSAYKNDFTDDEIKKIAALNYKDWGR LSKRFLTGIEGVDKTTGEKGSIIYFMREYNLNLMELMSGHYTFTEEVEKLNPVENRELCYEM VDELYLSPSVKRMLWQSLRWDEIKRI IGKDPKKIFIEMARAKEAKNSRKESRKNKLLEFYK FGKKAFINEIGEERYNYLLNEINSEEESKFRWDNLYLYYTQLGRCMYSLEPIDLADLKSNNI YDQDHIYPKSKIYDDSLENRVLVKKNLNHEKGNQYPIPEKVLNKNAYGFWKILFDKGLIGQK KYTRLTRRTPFEERELAEFIERQIVETRQATKETANLLKNICQDSEIVYSKAENASRFRQEF DIIKCRTVNDLHHMHDAYLNIWGNVYNTKFTKNPLNFIKDKDNVRSYNLENMFKYDWRGS YTAWIADDSEGNVKAATIKKVKRELEGKNYRFTRMSYIGTGGLYDQNLMRKGKGQIPQKENT NKSNIEKYGGYNKASSAYFALIESDGKAGRERTLETIPIMVYNQEKYGNTEAVDKYLKDNLE LQDPKILKDKIKINSLIKLDGFLYNIKGKTGDSLSIAGSVQLIVNKEEQKLIKKMDKFLVKK KDNKDIKVTSFDNIKEEELIKLYKTLSDKLNNGIYSNKRNNQAKNISEALDKFKEISIEEKI DVLNQIILLFQSYNNGCNLKSIGLSAKTGWFIPKKLNYKECKLINQSITGLFENEVDLLNL

SEQ ID NO: 319

MGKMYYLGLDIGTNSVGYAVTDPSYHLLKFKGEPMWGAHVFAAGNQSAERRSFRTSRRRLDR RQQRVKLVQEIFAPVISPIDPRFFIRLHESALWRDDVAETDKHIFFNDPTYTDKEYYSDYPT IHHLIVDLMESSEKHDPRLVYLAVAWLVAHRGHFLNEVDKDNIGDVLSFDAFYPEFLAFLSD NGVSPWVCESKALQATLLSRNSVNDKYKALKSLIFGSQKPEDNFDANISEDGLIQLLAGKKV KVNKLFPQESNDASFTLNDKEDAIEEILGTLTPDECEWIAHIRRLFDWAIMKHALKDGRTIS ESKVKLYEQHHHDLTQLKYFVKTYLAKEYDDIFRNVDSETTKNYVAYSYHVKEVKGTLPKNK ATQEEFCKYVLGKVKNIECSEADKVDFDEMIQRLTDNSFMPKQVSGENRVIPYQLYYYELKT ILNKAASYLPFLTQCGKDAISNQDKLLSIMTFRIPYFVGPLRKDNSEHAWLERKAGKIYPWN FNDKVDLDKSEEAFIRRMTNTCTYYPGEDVLPLDSLIYEKFMILNEINNIRIDGYPISVDVK QQVFGLFEKKRRVTVKDIQNLLLSLGALDKHGKLTGIDTTIHSNYNTYHHFKSLMERGVLTR DDVERIVERMTYSDDTKRVRLWLNNNYGTLTADDVKHISRLRKHDFGRLSKMFLTGLKGVHK ETGERASILDFMWNTNDNLMQLLSECYTFSDEITKLQEAYYAKAQLSLNDFLDSMYISNAVK RPIYRTLAWNDIRKACGTAPKRIFIEMARDGESKKKRSVTRREQIKNLYRSIRKDFQQEVD FLEKILENKSDGQLQSDALYLYFAQLGRDMYTGDPIKLEHIKDQSFYNIDHIYPQSMVKDDS LDNKVLVQSEINGEKSSRYPLDAAIRNKMKPLWDAYYNHGLISLKKYQRLTRSTPFTDDEKW DFINRQLVETRQSTKALAILLKRKFPDTEIVYSKAGLSSDFRHEFGLVKSRNINDLHHAKDA FLAIVTGNVYHERFNRRWFMVNQPYSVKTKTLFTHSIKNGNFVAWNGEEDLGRIVKMLKQNK NTIHFTRFSFDRKEGLFDIQPLKASTGLVPRKAGLDWKYGGYDKSTAAYYLLVRFTLEDKK TQHKLMMIPVEGLYKARIDHDKEFLTDYAQTTISEILQKDKQKVINIMFPMGTRHIKLNSMI SIDGFYLSIGGKSSKGKSVLCHAMVPLIVPHKIECYIKAMESFARKFKENNKLRIVEKFDKI TVEDNLNLYELFLQKLQHNPYNKFFSTQFDVLTNGRSTFTKLSPEEQVQTLLNILSIFKTCR SSGCDLKSINGSAQAARIMISADLTGLSKKYSDIRLVEQSASGLFVSKSQNLLEYL

SEQ ID NO: 320

MTKKEQPYNIGLDIGTSSVGWAVTNDNYDLLNIKKKNLWGVRLFEEAQTAKETRLNRSTRRR YRRRKNRINWLNEIFSEELAKTDPSFLIRLQNSWVSKKDPDRKRDKYNLFIDGPYTDKEYYR EFPTIFHLRKELILNKDKADIRLIYLALHNILKYRGNFTYEHQKFNISNLNNNLSKELIELN QQLIKYDISFPDDCDWNHISDILIGRGNATQKSSNILKDFTLDKETKKLLKEVINLILGNVA HLNTIFKTSLTKDEEKLNFSGKDIESKLDDLDSILDDDQFTVLDAANRIYSTITLNEILNGE SYFSMAKVNQYENHAIDLCKLRDMWHTTKNEEAVEQSRQAYDDYINKPKYGTKELYTSLKKF LKVALPTNLAKEAEEKISKGTYLVKPRNSENGWPYQLNKIEMEKIIDNQSQYYPFLKENKE KLLSILSFRIPYYVGPLQSAEKNPFAWMERKSNGHARPWNFDEIVDREKSSNKFIRRMTVTD SYLVGEPVLPKNSLIYQRYEVLNELNNIRITENLKTNPIGSRLTVETKQRIYNELFKKYKKV TVKKLTKWLIAQGYYKNPILIGLSQKDEFNSTLTTYLDMKKIFGSSFMEDNKNYDQIEELIE WLTIFEDKQILNEKLHSSKYSYTPDQIKKISNMRYKGWGRLSKKILMDITTETNTPQLLQLS NYSILDLMWATNNNFISIMSNDKYDFKNYIENHNLNKNEDQNISDLVNDIHVSPALKRGITQ SIKIVQEIVKFMGHAPKHIFIEVTRETKKSEITTSREKRIKRLQSKLLNKANDFKPQLREYL VPNKKIQEELKKHKNDLSSERIMLYFLQNGKSLYSEESLNINKLSDYQVDHILPRTYIPDDS LENKALVLAKENQRKADDLLLNSNVIDRNLERWTYMLNNNMIGLKKFKNLTRRVITDKDKLG FIHRQLVQTSQMVKGVANILDNMYKNQGTTCIQARANLSTAFRKALSGQDDTYHFKHPELVK NRNVNDFHHAQDAYLASFLGTYRLRRFPTNEMLLMNGEYNKFYGQVKELYSKKKKLPDSRKN GFI ISPLVNGTTQYDRNTGEIIWNVGFRDKILKIFNYHQCNVTRKTEIKTGQFYDQTIYSPK NPKYKKLIAQKKDMDPNIYGGFSGDNKSSITIVKIDNNKIKPVAIPIRLINDLKDKKTLQNW LEENVKHKKSIQIIKNNVPIGQI IYSKKVGLLSLNSDREVANRQQLILPPEHSALLRLLQIP DEDLDQILAFYDKNILVEILQELITKMKKFYPFYKGEREFLIANIENFNQATTSEKVNSLEE LITLLHANSTSAHLIFNNIEKKAFGRKTHGLTLNNTDFIYQSVTGLYETRIHIE SEQ ID NO: 321

MTKFNKNYSIGLDIGVSSVGYAVVTEDYRVPAFKFKVLGNTEKEKIKKNLIGSTTFVSAQPA KGTRVFRVNRRRIDRRNHRITYLRDIFQKEIEKVDKNFYRRLDESFRVLGDKSEDLQIKQPF FGDKELETAYHKKYPTIYHLRKHLADADKNSPVADIREVYMAISHILKYRGHFLTLDKINPN NINMQNSWIDFIESCQEVFDLEISDESKNIADIFKSSENRQEKVKKILPYFQQELLKKDKSI FKQLLQLLFGLKTKFKDCFELEEEPDLNFSKENYDENLENFLGSLEEDFSDVFAKLKVLRDT ILLSGMLTYTGATHARFSATMVERYEEHRKDLQRFKFFIKQNLSEQDYLDIFGRKTQNGFDV DKETKGYVGYITNKMVLTNPQKQKTIQQNFYDYISGKITGIEGAEYFLNKISDGTFLRKLRT SDNGAIPNQIHAYELEKIIERQGKDYPFLLENKDKLLSILTFKIPYYVGPLAKGSNSRFAWI KRATSSDILDDNDEDTRNGKIRPWNYQKLINMDETRDAFITNLIGNDIILLNEKVLPKRSLI YEEVMLQNELTRVKYKDKYGKAHFFDSELRQNIINGLFKNNSKRVNAKSLIKYLSDNHKDLN AIEIVSGVEKGKSFNSTLKTYNDLKTIFSEELLDSEIYQKELEEIIKVITVFDDKKSIKNYL TKFFGHLEILDEEKINQLSKLRYSGWGRYSAKLLLDIRDEDTGFNLLQFLRNDEENRNLTKL ISDNTLSFEPKIKDIQSKSTIEDDIFDEIKKLAGSPAIKRGILNSIKIVDELVQI IGYPPHN IVIEMARENMTTEEGQKKAKTRKTKLESALKNIENSLLENGKVPHSDEQLQSEKLYLYYLQN GKDMYTLDKTGSPAPLYLDQLDQYEVDHIIPYSFLPIDSIDNKVLTHRENNQQKLNNIPDKE TVANMKPFWEKLYNAKLISQTKYQRLTTSERTPDGVLTESMKAGFIERQLVETRQIIKHVAR ILDNRFSDTKI ITLKSQLITNFRNTFHIAKIRELNDYHHAHDAYLAVWGQTLLKVYPKLAP ELIYGHHAHFNRHEENKATLRKHLYSNIMRFFNNPDSKVSKDIWDCNRDLPIIKDVIYNSQI NFVKRTMIKKGAFYNQNPVGKFNKQLAANNRYPLKTKALCLDTSIYGGYGPMNSALSII IIA ERFNEKKGKIETVKEFHDIFIIDYEKFNNNPFQFLNDTSENGFLKKNNINRVLGFYRIPKYS LMQKIDGTRMLFESKSNLHKATQFKLTKTQNELFFHMKRLLTKSNLMDLKSKSAIKESQNFI LKHKEEFDNISNQLSAFSQKMLGNTTSLKNLIKGYNERKIKEIDIRDETIKYFYDNFIKMFS FVKSGAPKDINDFFDNKCTVARMRPKPDKKLLNATLIHQSITGLYETRIDLSKLGED SEQ ID NO: 322

MKQEYFLGLDMGTGSLGWAVTDSTYQVMRKHGKALWGTRLFESASTAEERRMFRTARRRLDR RNWRIQVLQEIFSEEISKVDPGFFLRMKESKYYPEDKRDAEGNCPELPYALFVDDNYTDKNY HKDYPTIYHLRKMLMETTEIPDIRLVYLVLHHMMKHRGHFLLSGDISQIKEFKSTFEQLIQN IQDEELEWHISLDDAAIQFVEHVLKDRNLTRSTKKSRLIKQLNAKSACEKAILNLLSGGTVK LSDIFNNKELDESERPKVSFADSGYDDYIGIVEAELAEQYYIIASAKAVYDWSVLVEILGNS VSISEAKIKVYQKHQADLKTLKKIVRQYMTKEDYKRVFVDTEEKLNNYSAYIGMTKKNGKKV DLKSKQCTQADFYDFLKKNVIKVIDHKEITQEIESEIEKENFLPKQVTKDNGVIPYQVHDYE LKKILDNLGTRMPFIKENAEKIQQLFEFRIPYYVGPLNRVDDGKDGKFTWSVRKSDARIYPW NFTEVIDVEASAEKFIRRMTNKCTYLVGEDVLPKDSLVYSKFMVLNELNNLRLNGEKISVEL KQRIYEELFCKYRKVTRKKLERYLVIEGIAKKGVEITGIDGDFKASLTAYHDFKERLTDVQL SQRAKEAIVLNWLFGDDKKLLKQRLSKMYPNLTTGQLKGICSLSYQGWGRLSKTFLEEITV PAPGTGEVWNIMTALWQTNDNLMQLLSRNYGFTNEVEEFNTLKKETDLSYKTVDELYVSPAV KRQIWQTLKWKEIQKVMGNAPKRVFVEMAREKQEGKRSDSRKKQLVELYRACKNEERDWIT ELNAQSDQQLRSDKLFLYYIQKGRCMYSGETIQLDELWDNTKYDIDHIYPQSKTMDDSLNNR VLVKKNYNAIKSDTYPLSLDIQKKMMSFWKMLQQQGFITKEKYVRLVRSDELSADELAGFIE RQIVETRQSTKAVATILKEALPDTEIVYVKAGNVSNFRQTYELLKVREMNDLHHAKDAYLNI WGNAYFVKFTKNAAWFIRNNPGRSYNLKRMFEFDIERSGEIAWKAGNKGSIVTVKKVMQKN NILVTRKAYEVKGGLFDQQIMKKGKGQVPIKGNDERLADIEKYGGYNKAAGTYFMLVKSLDK KGKEIRTIEFVPLYLKNQIEINHESAIQYLAQERGLNSPEILLSKIKIDTLFKVDGFKMWLS GRTGNQLIFKGANQLILSHQEAAILKGWKYVNRKNENKDAKLSERDGMTEEKLLQLYDTFL DKLSNTVYSIRLSAQIKTLTEKRAKFIGLSNEDQCIVLNEILHMFQCQSGSANLKLIGGPGS AGILVMNNNITACKQISVINQSPTGIYEKEIDLIKL

SEQ ID NO: 323

MKKPYSIGLDIGTNSVGWAWTDDYKVPAKKMKVLGNTDKSHIEKNLLGALLFDSGNTAEDR RLKRTARRRYTRRRNRILYLQEIFSEEMGKVDDSFFHRLEDSFLVTEDKRGERHPIFGNLEE EVKYHENFPTIYHLRQYLADNPEKVDLRLVYLALAHIIKFRGHFLIEGKFDTRNNDVQRLFQ EFLAVYDNTFENSSLQEQNVQVEEILTDKISKSAKKDRVLKLFPNEKSNGRFAEFLKLIVGN QADFKKHFELEEKAPLQFSKDTYEEELEVLLAQIGDNYAELFLSAKKLYDSILLSGILTVTD VGTKAPLSASMIQRYNEHQMDLAQLKQFIRQKLSDKYNEVFSDVSKDGYAGYIDGKTNQEAF YKYLKGLLNKIEGSGYFLDKIEREDFLRKQRTFDNGSIPHQIHLQEMRAIIRRQAEFYPFLA DNQDRIEKLLTFRIPYYVGPLARGKSDFAWLSRKSADKITPWNFDEIVDKESSAEAFINRMT NYDLYLPNQKVLPKHSLLYEKFTVYNELTKVKYKTEQGKTAFFDANMKQEIFDGVFKVYRKV TKDKLMDFLEKEFDEFRIVDLTGLDKENKVFNASYGTYHDLCKILDKDFLDNSKNEKILEDI VLTLTLFEDREMIRKRLENYSDLLTKEQVKKLERRHYTGWGRLSAELIHGIRNKESRKTILD YLIDDGNSNRNFMQLINDDALSFKEEIAKAQVIGETDNLNQWSDIAGSPAIKKGILQSLKI VDELVKIMGHQPENIWEMARENQFTNQGRRNSQQRLKGLTDSIKEFGSQILKEHPVENSQL QNDRLFLYYLQNGRDMYTGEELDIDYLSQYDIDHI IPQAFIKDNSIDNRVLTSSKENRGKSD DVPSKDVVRKMKSYWSKLLSAKLITQRKFDNLTKAERGGLTDDDKAGFIKRQLVETRQITKH VARILDERFNTETDENNKKIRQVKIVTLKSNLVSNFRKEFELYKVREINDYHHAHDAYLNAV IGKALLGVYPQLEPEFVYGDYPHFHGHKENKATAKKFFYSNIMNFFKKDDVRTDKNGEI IWK KDEHISNIKKVLSYPQVNIVKKVEEQTGGFSKESILPKGNSDKLIPRKTKKFYWDTKKYGGF DSPIVAYSILVIADIEKGKSKKLKTVKALVGVTIMEKMTFERDPVAFLERKGYRNVQEE il KLPKYSLFKLENGRKRLLASARELQKGNEIVLPNHLGTLLYHAKNIHKVDEPKHLDYVDKHK DEFKELLDWSNFSKKYTLAEGNLEKIKELYAQNNGEDLKELASSFINLLTFTAIGAPATFK FFDKNIDRKRYTSTTEILNATLIHQSITGLYETRIDLNKLGGD

SEQ ID NO: 324

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEAT RLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFI QLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGL TPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNT EITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEF YKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLK DNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT NFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIV LTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKW DELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQL QNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSD NVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAV VGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLAN GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNS DKLIARKKDWDPKKYGGFDSPTVAYSVLWAKVEKGKSKKLKSVKELLGITIMERSSFEKNP IDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI REQAENI IHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ LGGD

SEQ ID NO: 325

MTKPYSIGLDIGTNSVGWAVTTDNYKVPSKKMKVLGNTSKKYIKKNLLGVLLFDSGITAEGR RLKRTARRRYTRRRNRILYLQEIFSTEMATLDDAFFQRLDDSFLVPDDKRDSKYPIFGNLVE EKAYHDEFPTIYHLRKYLADSTKKADLRLVYLALAHMIKYRGHFLIEGEFNSKNNDIQKNFQ DFLDTYNAIFESDLSLENSKQLEEIVKDKISKLEKKDRILKLFPGEKNSGIFSEFLKLIVGN QADFRKCFNLDEKASLHFSKESYDEDLETLLGYIGDDYSDVFLKAKKLYDAILLSGFLTVTD NETEAPLSSAMIKRYNEHKEDLALLKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDF YVYLKKLLAEFEGADYFLEKIDREDFLRKQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLA KNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFEDVIDKESSAEAFINRMT SFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESMRDYQFLDSKQKKDIVRLYFKDKRK VTDKDIIEYLHAIYGYDGIELKGIEKQFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIH TLTIFEDREMIKQRLSKFENIFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYL IDDGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGNIKEWKSLPGSPAIKKGILQSIKI VDELVKVMGGRKPESIWEMARENQYTNQGKSNSQQRLKRLEKSLKELGSKILKENIPAKLS KIDNNALQNDRLYLYYLQNGKDMYTGDDLDIDRLSNYDIDHIIPQAFLKDNSIDNKVLVSSA SNRGKSDDVPSLEWKKRKTFWYQLLKSKLISQRKFDNLTKAERGGLSPEDKAGFIQRQLVE TRQITKHVARLLDEKFNNKKDENNRAVRTVKIITLKSTLVSQFRKDFELYKVREINDFHHAH DAYLNAVVASALLKKYPKLEPEFVYGDYPKYNSFRERKSATEKVYFYSNIMNIFKKSISLAD GRVIERPLIEVNEETGESVWNKESDLATVRRVLSYPQVNWKKVEEQNHGLDRGKPKGLFNA NLSSKPKPNSNENLVGAKEYLDPKKYGGYAGISNSFTVLVKGTIEKGAKKKITNVLEFQGIS ILDRINYRKDKLNFLLEKGYKDIELIIELPKYSLFELSDGSRRMLASILSTNNKRGEIHKGN QIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEELFYYILEFNENYVGAKKNGKLLN SAFQSWQNHSIDELCSSFIGPTGSERKGLFELTSRGSAADFEFLGVKIPRYRDYTPSSLLKD ATLIHQSVTGLYETRIDLAKLGEG

SEQ ID NO: 326

MKKQKFSDYYLGFDIGTNSVGWCVTDLDYNVLRFNKKDMWGSRLFDEAKTAAERRVQRNSRR RLKRRKWRLNLLEEIFSDEIMKIDSNFFRRLKESSLWLEDKNSKEKFTLFNDDNYKDYDFYK QYPTIFHLRDELIKNPEKKDIRLIYLALHSIFKSRGHFLFEGQNLKEIKNFETLYNNLISFL EDNGINKSIDKDNIEKLEKIICDSGKGLKDKEKEFKGIFNSDKQLVAIFKLSVGSSVSLNDL FDTDEYKKEEVEKEKISFREQIYEDDKPIYYSILGEKIELLDIAKSFYDFMVLNNILSDSNY ISEAKVKLYEEHKKDLKNLKYIIRKYNKENYDKLFKDKNENNYPAYIGLNKEKDKKEWEKS RLKIDDLIKVIKGYLPKPERIEEKDKTIFNEILNKIELKTILPKQRISDNGTLPYQIHEVEL EKILENQSKYYDFLNYEENGVSTKDKLLKTFKFRIPYYVGPLNSYHKDKGGNSWIVRKEEGK ILPWNFEQKVDIEKSAEEFIKRMTNKCTYLNGEDVIPKDSFLYSEYI ILNELNKVQVNDEFL NEENKRKIIDELFKENKKVSEKKFKEYLLVNQIANRTVELKGIKDSFNSNYVSYIKFKDIFG EKLNLDIYKEISEKSILWKCLYGDDKKIFEKKIKNEYGDILNKDEIKKINSFKFNTWGRLSE KLLTGIEFINLETGECYSSVMEALRRTNYNLMELLSSKFTLQESIDNENKEMNEVSYRDLIE ESYVSPSLKRAILQTLKIYEEIKKITGRVPKKVFIEMARGGDESMKNKKIPARQEQLKKLYD SCGNDIANFSIDIKEMKNSLSSYDNNSLRQKKLYLYYLQFGKCMYTGREIDLDRLLQNNDTY DIDHIYPRSKVIKDDSFDNLVLVLKNENAEKSNEYPVKKEIQEKMKSFWRFLKEKNFISDEK YKRLTGKDDFELRGFMARQLVNVRQTTKEVGKILQQIEPEIKIVYSKAEIASSFREMFDFIK VRELNDTHHAKDAYLNIVAGNVYNTKFTEKPYRYLQEIKENYDVKKIYNYDIKNAWDKENSL EIVKKNMEKNTVNITRFIKEEKGELFNLNPIKKGETSNEIISIKPKLYDGKDNKLNEKYGYY TSLKAAYFIYVEHEKKNKKVKTFERITRIDSTLIKNEKNLIKYLVSQKKLLNPKI IKKIYKE QTLIIDSYPYTFTGVDSNKKVELKNKKQLYLEKKYEQILKNALKFVEDNQGETEENYKFIYL KKRNNNEKNETIDAVKERYNIEFNEMYDKFLEKLSSKDYKNYINNKLYTNFLNSKEKFKKLK LWEKSLILREFLKIFNKNTYGKYEIKDSQTKEKLFSFPEDTGRIRLGQSSLGNNKELLEESV TGLFVKKIKL SEQ ID NO: 327

MKNYTIGLDIGVASVGWVCIDE YKILNYNNRHAFGVHEFESAESAAGRRLKRGMRRRYNRR KKRLQLLQSLFDSYITDSGFFSKTDSQHFWKNNNEFENRSLTEVLSSLRISSRKYPTIYHLR SDLIESNKKMDLRLVYLALHNLVKYRGHFLQEGNWSEAASAEGMDDQLLELVTRYAELENLS PLDLSESQWKAAETLLLNRNLTKTDQSKELTAMFGKEYEPFCKLVAGLGVSLHQLFPSSEQA LAYKETKTKVQLSNENVEEVMELLLEEESALLEAVQPFYQQWLYELLKGETYVAKAKVSAF KQYQKDMASLKNLLDKTFGEKVYRSYFISDKNSQREYQKSHKVEVLCKLDQFNKEAKFAETF YKDLKKLLEDKSKTSIGTTEKDEMLRI IKAIDSNQFLQKQKGIQNAAIPHQNSLYEAEKILR NQQAHYPFITTEWIEKVKQILAFRIPYYIGPLVKDTTQSPFSWVERKGDAPITPWNFDEQID KAASAEAFISRMRKTCTYLKGQEVLPKSSLTYERFEVLNELNGIQLRTTGAESDFRHRLSYE MKCWIIDNVFKQYKTVSTKRLLQELKKSPYADELYDEHTGEIKEVFGTQKENAFATSLSGYI SMKSILGAWDDNPAMTEELIYWIAVFEDREILHLKIQEKYPSITDVQRQKLALVKLPGWGR FSRLLIDGLPLDEQGQSVLDHMEQYSSVFMEVLKNKGFGLEKKIQKMNQHQVDGTKKIRYED IEELAGSPALKRGIWRSVKIVEELVSIFGEPANIVLEVAREDGEKKRTKSRKDQWEELTKTT LKNDPDLKSFIGEIKSQGDQRFNEQRFWLYVTQQGKCLYTGKALDIQNLSMYEVDHILPQNF VKDDSLDNLALVMPEANQRKNQVGQNKMPLEIIEANQQYAMRTLWERLHELKLISSGKLGRL KKPSFDEVDKDKFIARQLVETRQIIKHVRDLLDERFSKSDIHLVKAGIVSKFRRFSEIPKIR DYNNKHHAMDALFAAALIQSILGKYGKNFLAFDLSKKDRQKQWRSVKGSNKEFFLFKNFGNL RLQSPVTGEEVSGVEYMKHVYFELPWQTTKMTQTGDGMFYKESIFSPKVKQAKYVSPKTEKF VHDEVKNHSICLVEFTFMKKEKEVQETKFIDLKVIEHHQFLKEPESQLAKFLAEKETNSPII HARIIRTIPKYQKIWIEHFPYYFISTRELHNARQFEISYELMEKVKQLSERSSVEELKIVFG LLIDQMNDNYPIYTKSSIQDRVQKFVDTQLYDFKSFEIGFEELKKAVAANAQRSDTFGSRIS KKPKPEEVAIGYESITGLKYRKPRSWGTKR

SEQ ID NO: 328

MKKEIKDYFLGLDVGTGSVGWAVTDTDYKLLKANRKDLWGMRCFETAETAEVRRLHRGARRR IERRKKRIKLLQELFSQEIAKTDEGFFQRMKESPFYAEDKTILQENTLFNDKDFADKTYHKA YPTINHLIKAWIENKVKPDPRLLYLACHNIIKKRGHFLFEGDFDSENQFDTSIQALFEYLRE DMEVDIDADSQKVKEILKDSSLKNSEKQSRLNKILGLKPSDKQKKAITNLISGNKINFADLY DNPDLKDAEKNSISFSKDDFDALSDDLASILGDSFELLLKAKAVYNCSVLSKVIGDEQYLSF AKVKIYEKHKTDLTKLKNVIKKHFPKDYKKVFGYNKNEKNNNNYSGYVGVCKTKSKKLI INN SVNQEDFYKFLKTILSAKSEIKEVNDILTEIETGTFLPKQISKSNAEIPYQLRKMELEKILS NAEKHFSFLKQKDEKGLSHSEKI IMLLTFKIPYYIGPINDNHKKFFPDRCWWKKEKSPSGK TTPWNFFDHIDKEKTAEAFITSRTNFCTYLVGESVLPKSSLLYSEYTVLNEINNLQIIIDGK NICDIKLKQKIYEDLFKKYKKITQKQISTFIKHEGICNKTDEVIILGIDKECTSSLKSYIEL KNIFGKQVDEISTKNMLEEIIRWATIYDEGEGKTILKTKIKAEYGKYCSDEQIKKILNLKFS GWGRLSRKFLETVTSEMPGFSEPVNIITAMRETQNNLMELLSSEFTFTENIKKINSGFEDAE KQFSYDGLVKPLFLSPSVKKMLWQTLKLVKEISHITQAPPKKIFIEMAKGAELEPARTKTRL KILQDLYNNCKNDADAFSSEIKDLSGKIENEDNLRLRSDKLYLYYTQLGKCMYCGKPIEIGH VFDTSNYDIDHIYPQSKIKDDSISNRVLVCSSCNKNKEDKYPLKSEIQSKQRGFWNFLQRNN FISLEKLNRLTRATPISDDETAKFIARQLVETRQATKVAAKVLEKMFPETKIVYSKAETVSM FRNKFDIVKCREINDFHHAHDAYLNIVVGNVYNTKFTNNPWNFIKEKRDNPKIADTYNYYKV FDYDVKRN ITAWEKGKTI ITVKDMLKRNTPIYTRQAACKKGELFNQTIMKKGLGQHPLKKE GPFSNISKYGGYNKVSAAYYTLIEYEEKGNKIRSLETIPLYLVKDIQKDQDVLKSYLTDLLG KKEFKILVPKIKINSLLKINGFPCHITGKTNDSFLLRPAVQFCCSNNEVLYFKKI IRFSEIR SQREKIGKTISPYEDLSFRSYIKENLWKKTKNDEIGEKEFYDLLQKKNLEIYDMLLTKHKDT IYKKRPNSATIDILVKGKEKFKSLIIENQFEVILEILKLFSATRNVSDLQHIGGSKYSGVAK IGNKISSLDNCILIYQSITGIFEKRIDLLKV

SEQ ID NO: 329

MEGQMKNNGNNLQQGNYYLGLDVGTSSVGWAVTDTDYNVLKFRGKSMWGARLFDEASTAEER RTHRGNRRRLARRKYRLLLLEQLFEKEIRKIDDNFFVRLHESNLWADDKSKPSKFLLFNDTN FTDKDYLKKYPTIYHLRSDLIHNSTEHDIRLVFLALHHLIKYRGHFIYDNSANGDVKTLDEA VSDFEEYLNENDIEFNIENKKEFINVLSDKHLTKKEKKISLKKLYGDITDSENINISVLIEM LSGSSISLSNLFKDIEFDGKQNLSLDSDIEETLNDWDILGDNIDLLIHAKEVYDIAVLTSS LGKHKYLCDAKVELFEKNKKDLMILKKYIKKNHPEDYKKIFSSPTEKKNYAAYSQTNSKNVC SQEEFCLFIKPYIRDMVKSENEDEVRIAKEVEDKSFLTKLKGTNNSVVPYQIHERELNQILK NIVAYLPFMNDEQEDISWDKIKLIFKFKIPYYVGPLNTKSTRSWVYRSDEKIYPWNFSNVI DLDKTAHEFMNRLIGRCTYTNDPVLPMDSLLYSKYNVLNEINPIKVNGKAIPVEVKQAIYTD LFENSKKKVTRKSIYIYLLKNGYIEKEDIVSGIDIEIKSKLKSHHDFTQIVQENKCTPEEIE RIIKGILVYSDDKSMLRRWLKNNIKGLSENDVKYLAKLNYKEWGRLSKTLLTDIYTINPEDG EACSILDIMWNTNATLMEILSNEKYQFKQNIENYKAENYDEKQNLHEELDDMYISPAARRSI WQALRIVDEIVDIKKSAPKKIFIEMAREKKSAMKKKRTESRKDTLLELYKSCKSQADGFYDE ELFEKLSNESNSRLRRDQLYLYYTQMGRSMYTGKRIDFDKLINDKNTYDIDHIYPRSKIKDD SITNRVLVEKDINGEKTDIYPISEDIRQKMQPFWKILKEKGLINEEKYKRLTRNYELTDEEL SSFVARQLVETQQSTKALATLLKKEYPSAKIVYSKAGNVSEFRNRKDKELPKFREINDLHHA KDAYLNIWGNVYDTKFTEKFFNNIRNENYSLKRVFDFSVPGAWDAKGSTFNTIKKYMAKNN PIIAFAPYEVKGELFDQQIVPKGKGQFPIKQGKDIEKYGGYNKLSSAFLFAVEYKGKKARER SLETVYIKDVELYLQDPIKYCESVLGLKEPQIIKPKILMGSLFSINNKKLVVTGRSGKQYVC HHIYQLSINDEDSQYLKNIAKYLQEEPDGNIERQNILNITSVNNIKLFDVLCTKFNSNTYEI ILNSLKNDVNEGREKFSELDILEQCNILLQLLKAFKCNRESSNLEKLNNKKQAGVIVIPHLF TKCSVFKVIHQSITGLFEKEMDLLK

SEQ ID NO: 330

MGRKPYILSLDIGTGSVGYACMDKGFNVLKYHDKDALGVYLFDGALTAQERRQFRTSRRRKN RRIKRLGLLQELLAPLVQNPNFYQFQRQFAWKNDNMDFKNKSLSEVLSFLGYESKKYPTIYH LQEALLLKDEKFDPELIYMALYHLVKYRGHFLFDHLKIENLTNNDNMHDFVELIETYENLNN IKLNLDYEKTKVIYEILKDNEMTKNDRAKRVKNMEKKLEQFSIMLLGLKFNEGKLFNHADNA EELKGANQSHTFADNYEENLTPFLTVEQSEFIERANKIYLSLTLQDILKGKKSMAMSKVAAY DKFRNELKQVKDIVYKADSTRTQFKKIFVSSKKSLKQYDATPNDQTFSSLCLFDQYLIRPKK QYSLLIKELKKIIPQDSELYFEAENDTLLKVLNTTDNASIPMQINLYEAETILRNQQKYHAE ITDEMIEKVLSLIQFRIPYYVGPLVNDHTASKFGWMERKSNESIKPWNFDEWDRSKSATQF IRRMTNKCSYLINEDVLPKNSLLYQEMEVLNELNATQIRLQTDPKNRKYRMMPQIKLFAVEH IFKKYKTVSHSKFLEIMLNSNHRENFMNHGEKLSIFGTQDDKKFASKLSSYQDMTKIFGDIE GKRAQIEEIIQWITIFEDKKILVQKLKECYPELTSKQINQLKKLNYSGWGRLSEKLLTHAYQ GHSIIELLRHSDENFMEILTNDVYGFQNFIKEENQVQSNKIQHQDIANLTTSPALKKGIWST IKLVRELTSIFGEPEKIIMEFATEDQQKGKKQKSRKQLWDDNIKKNKLKSVDEYKYIIDVAN KLNNEQLQQEKLWLYLSQNGKCMYSGQSIDLDALLSPNATKHYEVDHIFPRSFIKDDSIDNK VLVIKKMNQTKGDQVPLQFIQQPYERIAYWKSLNKAGLISDSKLHKLMKPEFTAMDKEGFIQ RQLVETRQISVHVRDFLKEEYPNTKVIPMKAKMVSEFRKKFDIPKIRQMNDAHHAIDAYLNG WYHGAQLAYPNVDLFDFNFKWEKVREKWKALGEFNTKQKSRELFFFKKLEKMEVSQGERLI SKIKLDMNHFKINYSRKLANIPQQFYNQTAVSPKTAELKYESNKSNEWYKGLTPYQTYWA IKSVNKKGKEKMEYQMIDHYVFDFYKFQNGNEKELALYLAQRENKDEVLDAQIVYSLNKGDL LYINNHPCYFVSRKEVINAKQFELTVEQQLSLYNVMNNKETNVEKLLIEYDFIAEKVINEYH HYLNSKLKEKRVRTFFSESNQTHEDFIKALDELFKWTASATRSDKIGSRKNSMTHRAFLGK GKDVKIAYTSISGLKTTKPKSLFKLAESRNEL SEQ ID NO: 331

MAKILGLDLGTNSIGWAWERENIDFSLIDKGVRIFSEGVKSEKGIESSRAAERTGYRSARK IKYRRKLRKYETLKVLSLNRMCPLSIEEVEEWKKSGFKDYPLNPEFLKWLSTDEESNVNPYF FRDRASKHKVSLFELGRAFYHIAQRRGFLSNRLDQSAEGILEEHCPKIEAIVEDLISIDEIS TNITDYFFETGILDSNEKNGYAKDLDEGDKKLVSLYKSLLAILKKNESDFENCKSEIIERLN KKDVLGKVKGKIKDISQAMLDGNYKTLGQYFYSLYSKEKIRNQYTSREEHYLSEFITICKVQ GIDQINEEEKINEKKFDGLAKDLYKAIFFQRPLKSQKGLIGKCSFEKSKSRCAISHPDFEEY RMWTYLNTIKIGTQSDKKLRFLTQDEKLKLVPKFYRKNDFNFDVLAKELIEKGSSFGFYKSS KKNDFFYWFNYKPTDTVAACQVAASLKNAIGEDWKTKSFKYQTINSNKEQVSRTVDYKDLWH LLTVATSDVYLYEFAIDKLGLDEKNAKAFSKTKLKKDFASLSLSAINKILPYLKEGLLYSHA VFVA IE IVDE IWKDEKQRDYIKTQISEI IENYTLEKSRFEIINGLLKEYKSENEDGKRV YYSKEAEQSFENDLKKKLVLFYKSNEIENKEQQETIFNELLPIFIQQLKDYEFIKIQRLDQK VLIFLKGKNETGQIFCTEEKGTAEEKEKKIKNRLKKLYHPSDIEKFKKKIIKDEFGNEKIVL GSPLTPSIKNPMAMRALHQLRKVLNALILEGQIDEKTIIHIEMARELNDANKRKGIQDYQND NKKFREDAIKEIKKLYFEDCKKEVEPTEDDILRYQLWMEQNRSEIYEEGKNISICDIIGSNP AYDIEHTIPRSRSQDNSQMNKTLCSQRFNREVKKQSMPIELNNHLEILPRIAHWKEEADNLT REIEIISRSIKAAATKEIKDKKIRRRHYLTLKRDYLQGKYDRFIWEEPKVGFKNSQIPDTGI ITKYAQAYLKSYFKKVESVKGGMVAEFRKIWGIQESFIDENGMKHYKVKDRSKHTHHTIDAI TIACMTKEKYDVLAHAWTLEDQQNKKEARSI IEASKPWKTFKEDLLKIEEEILVSHYTPDNV KKQAKKIVRVRGKKQFVAEVERDVNGKAVPKKAASGKTIYKLDGEGKKLPRLQQGDTIRGSL HQDSIYGAIKNPLNTDEIKYVIRKDLESIKGSDVESIVDEWKEKIKEAIANKVLLLSSNAQ QKNKLVGTVWMNEEKRIAINKVRIYANSVKNPLHIKEHSLLSKSKHVHKQKVYGQNDENYAM AIYELDGKRDFELINIFNLAKLIKQGQGFYPLHKKKEIKGKIVFVPIEKRNKRDVVLKRGQQ WFYDKEVENPKDISEIVDFKGRIYIIEGLSIQRIVRPSGKVDEYGVIMLRYFKEARKADDI KQDNFKPDGVFKLGENKPTRKMNHQFTAFVEGIDFKVLPSGKFEKI

SEQ ID NO: 332

MEFKKVLGLDIGTNSIGCALLSLPKSIQDYGKGGRLEWLTSRVIPLDADYMKAFIDGKNGLP QVITPAGKRRQKRGSRRLKHRYKLRRSRLIRVFKTLNWLPEDFPLDNPKRIKETISTEGKFS FRISDYVPISDESYREFYREFGYPENEIEQVIEEINFRRKTKGKNKNPMIKLLPEDWWYYL RKKALIKPTTKEELIRIIYLFNQRRGFKSSRKDLTETAILDYDEFAKRLAEKEKYSAENYET KFVSITKVKEVVELKTDGRKGKKRFKVILEDSRIEPYEIERKEKPDWEGKEYTFLVTQKLEK GKFKQNKPDLPKEEDWALCTTALDNRMGSKHPGEFFFDELLKAFKEKRGYKIRQYPVNRWRY KKELEFIWTKQCQLNPELNNLNINKEILRKLATVLYPSQSKFFGPKIKEFENSDVLHIISED IIYYQRDLKSQKSLISECRYEKRKGIDGEIYGLKCIPKSSPLYQEFRIWQDIHNIKVIRKES EVNGKKKINIDETQLYINENIKEKLFELFNSKDSLSEKDILELISLNIINSGIKISKKEEET THRINLFANRKELKGNETKSRYRKVFKKLGFDGEYILNHPSKLNRLWHSDYSNDYADKEKTE KSILSSLGWKNRNGKWEKSKNYDVFNLPLEVAKAIANLPPLKKEYGSYSALAIRKMLWMRD GKYWQHPDQIAKDQENTSLMLFDKNLIQLTNNQRKVLNKYLLTLAEVQKRSTLIKQKLNEIE HNPYKLELVSDQDLEKQVLKSFLEKKNESDYLKGLKTYQAGYLIYGKHSEKDVPIVNSPDEL GEYIRKKLPNNSLRNPIVEQVIRETIFIVRDVWKSFGIIDEIHIELGRELKNNSEERKKTSE SQEKNFQEKERARKLLKELLNSSNFEHYDENGNKIFSSFTVNPNPDSPLDIEKFRIWKNQSG LTDEELNKKLKDEKIPTEIEVKKYILWLTQKCRSPYTGKIIPLSKLFDSNVYEIEHIIPRSK MKNDSTNNLVICELGVNKAKGDRLAANFISESNGKCKFGEVEYTLLKYGDYLQYCKDTFKYQ KAKYKNLLATEPPEDFIERQINDTRYIGRKLAELLTPWKDSKNIIFTIGSITSELKITWGL NGVWKDILRPRFKRLESIINKKLIFQDEDDPNKYHFDLSINPQLDKEGLKRLDHRHHALDAT IIAATTREHVRYLNSLNAADNDEEKREYFLSLCNHKIRDFKLPWENFTSEVKSKLLSCVVSY KESKPILSDPFNKYLKWEYKNGKWQKVFAIQIKNDRWKAVRRSMFKEPIGTVWIKKIKEVSL KEAIKIQAIWEEVKNDPVRKKKEKYIYDDYAQKVIAKIVQELGLSSSMRKQDDEKLNKFINE AKVSAGVNKNLNTTNKTIYNLEGRFYEKIKVAEYVLYKAKRMPLNKKEYIEKLSLQKMFNDL PNFILEKSILDNYPEILKELESDNKYI IEPHKKNNPVNRLLLEHILEYHNNPKEAFSTEGLE KLNKKAINKIGKPIKYITRLDGDINEEEIFRGAVFETDKGSNVYFVMYENNQTKDREFLKPN PSISVLKAIEHKNKIDFFAPNRLGFSRIILSPGDLVYVPTNDQYVLIKDNSSNETIINWDDN EFISNRIYQVKKFTGNSCYFLKNDIASLILSYSASNGVGEFGSQNISEYSVDDPPIRIKDVC IKIRVDRLGNVRPL SEQ ID NO: 333

MKHILGLDLGTNSIGWALIERNIEEKYGKIIGMGSRIVPMGAELSKFEQGQAQTKNADRRTN RGARRLNKRYKQRRNKLIYILQKLDMLPSQIKLKEDFSDPNKIDKITILPISKKQEQLTAFD LVSLRVKALTEKVGLEDLGKIIYKYNQLRGYAGGSLEPEKEDIFDEEQSKDKKNKSFIAFSK IVFLGEPQEEIFKNKKLNRRAIIVETEEGNFEGSTFLENIKVGDSLELLINISASKSGDTIT IKLPNKTNWRKKMENIENQLKEKSKEMGREFYISEFLLELLKENRWAKIRNNTILRARYESE FEAIWNEQVKHYPFLENLDKKTLIEIVSFIFPGEKESQKKYRELGLEKGLKYIIKNQWFYQ RELKDQSHLISDCRYEPNEKAIAKSHPVFQEYKVWEQINKLIVNTKIEAGTNRKGEKKYKYI DRPIPTALKEWIFEELQNKKEITFSAIFKKLKAEFDLREGIDFLNGMSPKDKLKGNETKLQL QKSLGELWDVLGLDSINRQIELWNILYNEKGNEYDLTSDRTSKVLEFINKYGNNIVDDNAEE TAIRISKIKFARAYSSLSLKAVERILPLVRAGKYFNNDFSQQLQSKILKLLNENVEDPFAKA AQTYLDNNQSVLSEGGVGNSIATILVYDKHTAKEYSHDELYKSYKEINLLKQGDLRNPLVEQ IINEALVLIRDIWKNYGIKPNEIRVELARDLKNSAKERATIHKRNKDNQTINNKIKETLVKN KKELSLANIEKVKLWEAQRHLSPYTGQPIPLSDLFDKEKYDVDHIIPISRYFDDSFTNKVIS EKSVNQEKANRTAMEYFEVGSLKYSIFTKEQFIAHVNEYFSGVKRKNLLATSIPEDPVQRQI KDTQYIAIRVKEELNKIVGNENVKTTTGSITDYLRNHWGLTDKFKLLLKERYEALLESEKFL EAEYDNYKKDFDSRKKEYEEKEVLFEEQELTREEFIKEYKENYIRYKKNKLIIKGWSKRIDH RHHAIDALIVACTEPAHIKRLNDLNKVLQDWLVEHKSEFMPNFEGSNSELLEEILSLPENER TEIFTQIEKFRAIEMPWKGFPEQVEQKLKEI IISHKPKDKLLLQYNKAGDRQIKLRGQLHEG TLYGISQGKEAYRIPLTKFGGSKFATEKNIQKIVSPFLSGFIANHLKEYNNKKEEAFSAEGI MDLNNKLAQYRNEKGELKPHTPISTVKIYYKDPSKNKKKKDEEDLSLQKLDREKAFNEKLYV KTGDNYLFAVLEGEIKTKKTSQIKRLYDIISFFDATNFLKEEFRNAPDKKTFDKDLLFRQYF EERNKAKLLFTLKQGDFVYLPNENEEVILDKESPLYNQYWGDLKERGKNIYWQKFSKKQIY FIKHTIADIIKKDVEFGSQNCYETVEGRSIKENCFKLEIDRLGNIVKVIKR SEQ ID NO: 334

MHVEIDFPHFSRGDSHLAMNKNEILRGSSVLYRLGLDLGSNSLGWFVTHLEKRGDRHEPVAL GPGGVRIFPDGRDPQSGTSNAVDRRMARGARKRRDRFVERRKELIAALIKYNLLPDDARERR ALEVLDPYALRKTALTDTLPAHHVGRALFHLNQRRGFQSNRKTDSKQSEDGAIKQAASRLAT DKGNETLGVFFADMHLRKSYEDRQTAIRAELVRLGKDHLTGNARKKIWAKVRKRLFGDEVLP RADAPHGVRARATITGTKASYDYYPTRDMLRDEFNAIWAGQSAHHATITDEARTEIEHI IFY QRPLKPAIVGKCTLDPATRPFKEDPEGYRAPWSHPLAQRFRILSEARNLEIRDTGKGSRRLT KEQSDLVVAALLANREVKFDKLRTLLKLPAEARFNLESDRRAALDGDQTAARLSDKKGFNKA WRGFPPERQIAIVARLEETEDENELIAWLEKECALDGAAAARVANTTLPDGHCRLGLRAIKK IVPIMQDGLDEDGVAGAGYHIAAKRAGYDHAKLPTGEQLGRLPYYGQWLQDAWGSGDARDQ KEKQYGQFPNPTVHIGLGQLRRVVNDLIDKYGPPTEISIEFTRALKLSEQQKAERQREQRRN QDKNKARAEELAKFGRPANPRNLLKMRLWEELAHDPLDRKCVYTGEQISIERLLSDEVDIDH ILPVAMTLDDSPANKIICMRYANRHKRKQTPSEAFGSSPTLQGHRYNWDDIAARATGLPRNK RWRFDANAREEFDKRGGFLARQLNETGWLARLAKQYLGAVTDPNQIWWPGRLTSMLRGKWG LNGLLPSDNYAGVQDKAEEFLASTDDMEFSGVKNRADHRHHAIDGLVTALTDRSLLWKMANA YDEEHEKFVIEPPWPTMRDDLKAALEKMWSHKPDHGIEGKLHEDSAYGFVKPLDATGLKEE EAGNLVYRKAIESLNENEVDRIRDIQLRTIVRDHVNVEKTKGVALADALRQLQAPSDDYPQF KHGLRHVRILKKEKGDYLVPIANRASGVAYKAYSAGENFCVEVFETAGGKWDGEAVRRFDAN KKNAGPKIAHAPQWRDANEGAKLVMRIHKGDLIRLDHEGRARIMWHRLDAAAGRFKLADHN ETGNLDKRHATNNDIDPFRWLMASYNTLKKLAAVPVRVDELGRVWRVMPN SEQ ID NO: 335

METTLGIDLGTNSIGLALVDQEEHQILYSGVRIFPEGINKDTIGLGEKEESRNATRRAKRQM RRQYFRKKLRKAKLLELLIAYDMCPLKPEDVRRWKNWDKQQKSTVRQFPDTPAFREWLKQNP YELRKQAVTEDVTRPELGRILYQMIQRRGFLSSRKGKEEGKIFTGKDRMVGIDETRKNLQKQ TLGAYLYDIAPKNGEKYRFRTERVRARYTLRDMYIREFEIIWQRQAGHLGLAHEQATRKKNI FLEGSATNVRNSKLITHLQAKYGRGHVLIEDTRITVTFQLPLKEVLGGKIEIEEEQLKFKSN ESVLFWQRPLRSQKSLLSKCVFEGRNFYDPVHQKWIIAGPTPAPLSHPEFEEFRAYQFINNI IYGKNEHLTAIQREAVFELMCTESKDFNFEKIPKHLKLFEKFNFDDTTKVPACTTISQLRKL FPHPVWEEKREEIWHCFYFYDDNTLLFEKLQKDYALQTNDLEKIKKIRLSESYGNVSLKAIR RINPYLKKGYAYSTAVLLGGIRNSFGKRFEYFKEYEPEIEKAVCRILKEKNAEGEVIRKIKD YLVHNRFGFAKNDRAFQKLYHHSQAITTQAQKERLPETGNLRNPIVQQGLNELRRTVNKLLA TCREKYGPSFKFDHIHVEMGRELRSSKTEREKQSRQIRENEKKNEAAKVKLAEYGLKAYRDN IQKYLLYKEIEEKGGTVCCPYTGKTL ISHTLGSDNSVQIEHI IPYSISLDDSLANKTLCDA TFNREKGELTPYDFYQKDPSPEKWGASSWEEIEDRAFRLLPYAKAQRFIRRKPQESNEFISR QLNDTRYISKKAVEYLSAICSDVKAFPGQLTAELRHLWGLNNILQSAPDITFPLPVSATENH REYYVITNEQNEVIRLFPKQGETPRTEKGELLLTGEVERKVFRCKGMQEFQTDVSDGKYWRR IKLSSSVTWSPLFAPKPISADGQIVLKGRIEKGVFVCNQLKQKLKTGLPDGSYWISLPVISQ TFKEGESVNNSKLTSQQVQLFGRVREGIFRCHNYQCPASGADGNFWCTLDTDTAQPAFTPIK NAPPGVGGGQI ILTGDVDDKGIFHADDDLHYELPASLPKGKYYGIFTVESCDPTLIPIELSA PKTSKGENLIEGNIWVDEHTGEVRFDPKKNREDQRHHAIDAIVIALSSQSLFQRLSTYNARR ENKKRGLDSTEHFPSPWPGFAQDVRQSWPLLVSYKQNPKTLCKISKTLYKDGKKIHSCGNA VRGQLHKETVYGQRTAPGATEKSYHIRKDIRELKTSKHIGKWDITIRQMLLKHLQENYHID ITQEFNIPSNAFFKEGVYRIFLPNKHGEPVPIKKIRMKEELGNAERLKDNINQYVNPRNNHH VMIYQDADGNLKEEIVSFWSVIERQNQGQPIYQLPREGRNIVSILQINDTFLIGLKEEEPEV YRNDLSTLSKHLYRVQKLSGMYYTFRHHLASTLNNEREEFRIQSLEAWKRANPVKVQIDEIG RITFLNGPLC

SEQ ID NO: 336

MESSQILSPIGIDLGGKFTGVCLSHLEAFAELPNHANTKYSVILIDHNNFQLSQAQRRATRH RVRNKKRNQFVKRVALQLFQHILSRDLNAKEETALCHYLNNRGYTYVDTDLDEYIKDETTIN LLKELLPSESEHNFIDWFLQKMQSSEFRKILVSKVEEKKDDKELKNAVKNIKNFITGFEKNS VEGHRHRKVYFENIKSDITKDNQLDSIKKKIPSVCLSNLLGHLSNLQWKNLHRYLAKNPKQF DEQTFGNEFLRMLKNFRHLKGSQESLAVRNLIQQLEQSQDYISILEKTPPEITIPPYEARTN TGMEKDQSLLLNPEKLNNLYPNWRNLIPGIIDAHPFLEKDLEHTKLRDRKRIISPSKQDEKR DSYILQRYLDLNKKIDKFKIKKQLSFLGQGKQLPANLIETQKEMETHFNSSLVSVLIQIASA YNKEREDAAQGIWFDNAFSLCELSNINPPRKQKILPLLVGAILSEDFINNKDKWAKFKIFWN THKIGRTSLKSKCKEIEEARKNSGNAFKIDYEEALNHPEHSNNKALIKIIQTIPDIIQAIQS HLGHNDSQALIYHNPFSLSQLYTILETKRDGFHKNCVAVTCENYWRSQKTEIDPEISYASRL PADSVRPFDGVLARMMQRLAYEIAMAKWEQIKHIPDNSSLLIPIYLEQNRFEFEESFKKIKG SSSDKTLEQAIEKQNIQWEEKFQRIINASMNICPYKGASIGGQGEIDHIYPRSLSKKHFGVI FNSEVNLIYCSSQGNREKKEEHYLLEHLSPLYLKHQFGTDNVSDIKNFISQNVANIKKYISF HLLTPEQQKAARHALFLDYDDEAFKTITKFLMSQQKARVNGTQKFLGKQIMEFLSTLADSKQ LQLEFSIKQITAEEVHDHRELLSKQEPKLVKSRQQSFPSHAIDATLTMSIGLKEFPQFSQEL DNSWFINHLMPDEVHLNPVRSKEKYNKPNISSTPLFKDSLYAERFIPVWVKGETFAIGFSEK DLFEIKPSNKEKLFTLLKTYSTKNPGESLQELQAKSKAKWLYFPINKTLALEFLHHYFHKEI VTPDDTTVCHFINSLRYYTKKESITVKILKEPMPVLSVKFESSKKNVLGSFKHTIALPATKD WERLFNHPNFLALKANPAPNPKEFNEFIRKYFLSDNNPNSDIPNNGHNIKPQKHKAVRKVFS LPVIPGNAGTMMRIRRKDNKGQPLYQLQTIDDTPSMGIQINEDRLVKQEVLMDAYKTRNLST IDGINNSEGQAYATFDNWLTLPVSTFKPEIIKLEMKPHSKTRRYIRITQSLADFIKTIDEAL MIKPSDSIDDPLNMPNEIVCKNKLFGNELKPRDGKMKIVSTGKIVTYEFESDSTPQWIQTLY VTQLKKQP

SEQ ID NO: 337

MKKIVGLDLGTNSIGWALINAYINKEHLYGIEACGSRIIPMDAAILGNFDKGNSISQTADRT SYRGIRRLRERHLLRRERLHRILDLLGFLPKHYSDSLNRYGKFLNDIECKLPWVKDETGSYK FIFQESFKEMLANFTEHHPILIANNKKVPYDWTIYYLRKKALTQKISKEELAWILLNFNQKR GYYQLRGEEEETPNKLVEYYSLKVEKVEDSGERKGKDTWYNVHLENGMIYRRTSNIPLDWEG KTKEFIVTTDLEADGSPKKDKEGNIKRSFRAPKDDDWTLIKKKTEADIDKIKMTVGAYIYDT LLQKPDQKIRGKLVRTIERKYYKNELYQILKTQSEFHEELRDKQLYIACLNELYPNNEPRRN SISTRDFCHLFIEDI IFYQRPLKSKKSLIDNCPYEENRYIDKESGEIKHASIKCIAKSHPLY QEFRLWQFIVNLRIYRKETDVDVTQELLPTEADYVTLFEWLNEKKEIDQKAFFKYPPFGFKK TTSNYRWNYVEDKPYPCNETHAQIIARLGKAHIPKAFLSKEKEETLWHILYSIEDKQEIEKA LHSFANKNNLSEEFIEQFKNFPPFKKEYGSYSAKAIKKLLPLMRMGKYWSIENIDNGTRIRI NKI IDGEYDE IRERVRQKAINLTDITHFRALPLWLACYLVYDRHSEVKDIVKWKTPKDIDL YLKSFKQHSLRNPIVEQVITETLRTVRDIWQQVGHIDEIHIELGREMKNPADKRARMSQQMI KNENTNLRIKALLTEFLNPEFGIENVRPYSPSQQDLLRIYEEGVLNSILELPEDIGIILGKF NQTDTLKRPTRSEILRYKLWLEQKYRSPYTGEMIPLSKLFTPAYEIEHIIPQSRYFDDSLSN KVICESEINKLKDRSLGYEFIKNHHGEKVELAFDKPVEVLSVEAYEKLVHESYSHNRSKMKK LLMEDIPDQFIERQLNDSRYISKWKSLLSNIVREENEQEAISKNVIPCTGGITDRLKKDWG INDVWNKIVLPRFIRLNELTESTRFTSINTNNTMIPSMPLELQKGFNKKRIDHRHHAMDAII IACANRNIVNYLNNVSASKNTKITRRDLQTLLCHKDKTDNNGNYKWVIDKPWETFTQDTLTA LQKITVSFKQNLRVINKTTNHYQHYENGKKIVSNQSKGDSWAIRKSMHKETVHGEVNLRMIK TVSFNEALKKPQAIVEMDLKKKILAMLELGYDTKRIKNYFEENKDTWQDINPSKIKVYYFTK ETKDRYFAVRKPIDTSFDKKKIKESITDTGIQQIMLRHLETKDNDPTLAFSPDGIDEMNRNI LILNKGKKHQPIYKVRVYEKAEKFTVGQKGNKRTKFVEAAKGTNLFFAIYETEEIDKDTKKV IRKRSYSTIPLNWIERQKQGLSSAPEDENGNLPKYILSPNDLVYVPTQEEINKGEWMPID RDRIYKMVDSSGITANFIPASTANLIFALPKATAEIYCNGENCIQNEYGIGSPQSKNQKAIT GEMVKEICFPIKVDRLGNI IQVGSCILTN

SEQ ID NO: 338

MSRSLTFSFDIGYASIGWAVIASASHDDADPSVCGCGTVLFPKDDCQAFKRREYRRLRRNIR SRRVRIERIGRLLVQAQIITPEMKETSGHPAPFYLASEALKGHRTLAPIELWHVLRWYAHNR GYDNNASWSNSLSEDGGNGEDTERVKHAQDLMDKHGTATMAETICRELKLEEGKADAPMEVS TPAYKNLNTAFPRLIVEKEVRRILELSAPLIPGLTAEIIELIAQHHPLTTEQRGVLLQHGIK LARRYRGSLLFGQLIPRFDNRIISRCPVTWAQVYEAELKKGNSEQSARERAEKLSKVPTANC PEFYEYRMARILCNIRADGEPLSAEIRRELMNQARQEGKLTKASLEKAISSRLGKETETNVS NYFTLHPDSEEALYLNPAVEVLQRSGIGQILSPSVYRIAANRLRRGKSVTPNYLLNLLKSRG ESGEALEKKIEKESKKKEADYADTPLKPKYATGRAPYARTVLKKWEEILDGEDPTRPARGE AHPDGELKAHDGCLYCLLDTDSSVNQHQKERRLDTMTNNHLVRHRMLILDRLLKDLIQDFAD GQKDRISRVCVEVGKELTTFSAMDSKKIQRELTLRQKSHTDAVNRLKRKLPGKALSANLIRK CRIAMDMNWTCPFTGATYGDHELENLELEHIVPHSFRQSNALSSLVLTWPGVNRMKGQRTGY DFVEQEQENPVPDKPNLHICSLNNYRELVEKLDDKKGHEDDRRRKKKRKALLMVRGLSHKHQ SQNHEAMKEIGMTEGMMTQSSHLMKLACKSIKTSLPDAHIDMIPGAVTAEVRKAWDVFGVFK ELCPEAADPDSGKILKENLRSLTHLHHALDACVLGLIPYIIPAHHNGLLRRVLAMRRIPEKL IPQVRPVANQRHYVLNDDGRMMLRDLSASLKENIREQLMEQRVIQHVPADMGGALLKETMQR VLSVDGSGEDAMVSLSKKKDGKKEKNQVKASKLVGVFPEGPSKLKALKAAIEIDGNYGVALD PKPWIRHIKVFKRIMALKEQNGGKPVRILKKGMLIHLTSSKDPKHAGVWRIESIQDSKGGV KLDLQRAHCAVPKNKTHECNWREVDLISLLKKYQMKRYPTSYTGTPR SEQ ID NO: 339

MTQKVLGLDLGTNSIGSAVRNLDLSDDLQWQLEFFSSDIFRSSVNKESNGREYSLAAQRSAH RRSRGLNEVRRRRLWATLNLLIKHGFCPMSSESLMRWCTYDKRKGLFREYPIDDKDFNAWIL LDFNGDGRPDYSSPYQLRRELVTRQFDFEQPIERYKLGRALYHIAQHRGFKSSKGETLSQQE TNSKPSSTDEIPDVAGAMKASEEKLSKGLSTYMKEHNLLTVGAAFAQLEDEGVRVRNNNDYR AIRSQFQHEIETIFKFQQGLSVESELYERLISEKKNVGTIFYKRPLRSQRGNVGKCTLERSK PRCAIGHPLFEKFRAWTLINNIKVRMSVDTLDEQLPMKLRLDLYNECFLAFVRTEFKFEDIR KYLEKRLGIHFSYNDKTINYKDSTSVAGCPITARFRKMLGEEWESFRVEGQKERQAHSKN I SFHRVSYSIEDIWHFCYDAEEPEAVLAFAQETLRLERKKAEELVRIWSAMPQGYAMLSQKAI RNINKILMLGLKYSDAVILAKVPELVDVSDEELLSIAKDYYLVEAQVNYDKRINSIVNGLIA KYKSVSEEYRFADHNYEYLLDESDEKDIIRQIENSLGARRWSLMDANEQTDILQKVRDRYQD FFRSHERKFVESPKLGESFENYLTKKFPMVEREQWKKLYHPSQITIYRPVSVGKDRSVLRLG NPDIGAIKNPTVLRVLNTLRRRVNQLLDDGVISPDETRVWETARELNDANRKWALDTYNRI RHDENEKIKKILEEFYPKRDGISTDDIDKARYVIDQREVDYFTGSKTYNKDIKKYKFWLEQG GQCMYTGRTINLSNLFDPNAFDIEHTIPESLSFDSSDMNLTLCDAHYNRFIKKNHIPTDMPN YDKAITIDGKEYPAITSQLQRWVERVERLNRNVEYWKGQARRAQNKDRKDQCMREMHLWKME LEYWKKKLERFTVTEVTDGFKNSQLVDTRVITRHAVLYLKSIFPHVDVQRGDVTAKFRKILG IQSVDEKKDRSLHSHHAIDATTLTIIPVSAKRDRMLELFAKIEEINKMLSFSGSEDRTGLIQ ELEGLKNKLQMEVKVCRIGHNVSEIGTFINDNIIVNHHIKNQALTPVRRRLRKKGYIVGGVD NPRWQTGDALRGEIHKASYYGAITQFAKDDEGKVLMKEGRPQVNPTIKFVIRRELKYKKSAA DSGFASWDDLGKAIVDKELFALMKGQFPAETSFKDACEQGIYMIKKGKNGMPDIKLHHIRHV RCEAPQSGLKIKEQTYKSEKEYKRYFYAAVGDLYAMCCYTNGKIREFRIYSLYDVSCHRKSD IEDIPEFITDKKGNRLMLDYKLRTGDMILLYKDNPAELYDLDNVNLSRRLYKINRFESQSNL VLMTHHLSTSKERGRSLGKTVDYQNLPESIRSSVKSLNFLIMGENRDFVIKNGKI IFNHR

SEQ ID NO: 340

MLVSPISVDLGGKNTGFFSFTDSLDNSQSGTVIYDESFVLSQVGRRSKRHSKRNNLRNKLVK RLFLLILQEHHGLSIDVLPDEIRGLFNKRGYTYAGFELDEKKKDALESDTLKEFLSEKLQSI DRDSDVEDFLNQIASNAESFKDYKKGFEAVFASATHSPNKKLELKDELKSEYGENAKELLAG LRVTKEILDEFDKQENQGNLPRAKYFEELGEYIATNEKVKSFFDSNSLKLTDMTKLIGNISN YQLKELRRYFNDKEMEKGDIWIPNKLHKITERFVRSWHPKNDADRQRRAELMKDLKSKEIME LLTTTEPVMTIPPYDDMNNRGAVKCQTLRLNEEYLDKHLPNWRDIAKRLNHGKFNDDLADST VKGYSEDSTLLHRLLDTSKEIDIYELRGKKPNELLVKTLGQSDANRLYGFAQNYYELIRQKV RAGIWVPVKNKDDSLNLEDNSNMLKRCNHNPPHKKNQIHNLVAGILGVKLDEAKFAEFEKEL WSAKVGNKKLSAYCKNIEELRKTHGNTFKIDIEELRKKDPAELSKEEKAKLRLTDDVILNEW SQKIANFFDIDDKHRQRFNNLFSMAQLHTVIDTPRSGFSSTCKRCTAENRFRSETAFYNDET GEFHKKATATCQRLPADTQRPFSGKIERYIDKLGYELAKIKAKELEGMEAKEIKVPIILEQN AFEYEESLRKSKTGSNDRVINSKKDRDGKKLAKAKENAEDRLKDKDKRIKAFSSGICPYCGD TIGDDGEIDHILPRSHTLKIYGTVFNPEGNLIYVHQKCNQAKADSIYKLSDIKAGVSAQWIE EQVANIKGYKTFSVLSAEQQKAFRYALFLQNDNEAYKKVVDWLRTDQSARVNGTQKYLAKKI QEKLTKMLPNKHLSFEFILADATEVSELRRQYARQNPLLAKAEKQAPSSHAIDAVMAFVARY QKVFKDGTPPNADEVAKLAMLDSWNPASNEPLTKGLSTNQKIEKMIKSGDYGQKNMREVFGK SIFGENAIGERYKPIWQEGGYYIGYPATVKKGYELKNCKWTSKNDIAKLEKIIKNQDLIS LKENQYIKIFSINKQTISELSNRYFNMNYKNLVERDKEIVGLLEFIVENCRYYTKKVDVKFA PKYIHETKYPFYDDWRRFDEAWRYLQENQNKTSSKDRFVIDKSSLNEYYQPDKNEYKLDVDT QPIWDDFCRWYFLDRYKTANDKKSIRIKARKTFSLLAESGVQGKVFRAKRKIPTGYAYQALP MDNNVIAGDYANILLEANSKTLSLVPKSGISIEKQLDKKLDVIKKTDVRGLAIDNNSFFNAD FDTHGIRLIVENTSVKVGNFPISAIDKSAKRMIFRALFEKEKGKRKKKTTISFKESGPVQDY LKVFLKKIVKIQLRTDGSISNIVVRKNAADFTLSFRSEHIQKLLK SEQ ID NO: 341

MAYRLGLDIGITSVGWAWALEKDESGLKPVRIQDLGVRIFDKAEDSKTGASLALPRREARS ARRRTRRRRHRLWRVKRLLEQHGILSMEQIEALYAQRTSSPDVYALRVAGLDRCLIAEEIAR VLIHIAHRRGFQSNRKSEIKDSDAGKLLKAVQENENLMQSKGYRTVAEMLVSEATKTDAEGK LVHGKKHGYVSNVRNKAGEYRHTVSRQAIVDEVRKIFAAQRALGNDVMSEELEDSYLKILCS QRNFDDGPGGDSPYGHGSVSPDGVRQSIYERMVGSCTFETGEKRAPRSSYSFERFQLLTKW NLRIYRQQEDGGRYPCELTQTERARVIDCAYEQTKITYGKLRKLLDMKDTESFAGLTYGLNR SRNKTEDTVFVEMKFYHEVRKALQRAGVFIQDLSIETLDQIGWILSVWKSDDNRRKKLSTLG LSDNVIEELLPLNGSKFGHLSLKAIRKILPFLEDGYSYDVACELAGYQFQGKTEYVKQRLLP PLGEGEVTNPVVRRALSQAIKWNAVIRKHGSPESIHIELARELSKNLDERRKIEKAQKENQ KNNEQIKDEIREILGSAHVTGRDIVKYKLFKQQQEFCMYSGEKLDVTRLFEPGYAEVDHIIP YGISFDDSYDNKVLVKTEQNRQKGNRTPLEYLRDKPEQKAKFIALVESIPLSQKKKNHLLMD KRAIDLEQEGFRERNLSDTRYITRALMNHIQAWLLFDETASTRSKRVVCVNGAVTAYMRARW GLTKDRDAGDKHHAADAWVACIGDSLIQRVTKYDKFKRNALADRNRYVQQVSKSEGITQYV DKETGEVFTWESFDERKFLPNEPLEPWPFFRDELLARLSDDPSKNIRAIGLLTYSETEQIDP IFVSRMPTRKVTGAAHKETIRSPRIVKVDDNKGTEIQWVSKVALTELKLTKDGEIKDYFRP EDDPRLYNTLRERLVQFGGDAKAAFKEPVYKISKDGSVRTPVRKVKIQEKLTLGVPVHGGRG IAENGGMVRIDVFAKGGKYYFVPIYVADVLKRELPNRLATAHKPYSEWRWDDSYQFKFSLY PNDAVMIKPSREVDITYKDRKEPVGCRIMYFVSANIASASISLRTHDNSGELEGLGIQGLEV FEKYWGPLGDTHPVYKERRMPFRVERKMN

SEQ ID NO: 342

MPVLSPLSPNAAQGRRRWSLALDIGEGSIGWAVAEVDAEGRVLQLTGTGVTLFPSAWSNENG TYVAHGAADRAVRGQQQRHDSRRRRLAGLARLCAPVLERSPEDLKDLTRTPPKADPRAIFFL RADAARRPLDGPELFRVLHHMAAHRGIRLAELQEVDPPPESDADDAAPAATEDEDGTRRAAA DERAFRRLMAEHMHRHGTQPTCGEIMAGRLRETPAGAQPVTRARDGLRVGGGVAVPTRALIE QEFDAIRAIQAPRHPDLPWDSLRRLVLDQAPIAVPPATPCLFLEELRRRGETFQGRTITREA IDRGLTVDPLIQALRIRETVGNLRLHERITEPDGRQRYVPRAMPELGLSHGELTAPERDTLV RALMHDPDGLAAKDGRIPYTRLRKLIGYDNSPVCFAQERDTSGGGITVNPTDPLMARWIDGW VDLPLKARSLYVRDVVARGADSAALARLLAEGAHGVPPVAAAAVPAATAAILESDIMQPGRY SVCPWAAEAILDAWANAPTEGFYDVTRGLFGFAPGEIVLEDLRRARGALLAHLPRTMAAART PNRAAQQRGPLPAYESVIPSQLITSLRRAHKGRAADWSAADPEERNPFLRTWTGNAATDHIL NQVRKTANEVITKYGNRRGWDPLPSRITVELAREAKHGVIRRNEIAKENRENEGRRKKESAA LDTFCQDNTVSWQAGGLPKERAALRLRLAQRQEFFCPYCAERPKLRATDLFSPAETEIDHVI ERRMGGDGPDNLVLAHKDCNNAKGKKTPHEHAGDLLDSPALAALWQGWRKENADRLKGKGHK ARTPREDKDFMDRVGWRFEEDARAKAEENQERRGRRMLHDTARATRLARLYLAAAVMPEDPA EIGAPPVETPPSPEDPTGYTAIYRTISRVQPVNGSVTHMLRQRLLQRDKNRDYQTHHAEDAC LLLLAGPAWQAFNTEAAQHGADAPDDRPVDLMPTSDAYHQQRRARALGRVPLATVDAALAD IVMPESDRQDPETGRVHWRLTRAGRGLKRRIDDLTRNCVILSRPRRPSETGTPGALHNATHY GRREITVDGRTDTWTQRMNARDLVALLDNAKIVPAARLDAAAPGDTILKEICTEIADRHDR WDPEGTHARRWISARLAALVPAHAEAVARDIAELADLDALADADRTPEQEARRSALRQSPY LGRAISAKKADGRARAREQEILTRALLDPHWGPRGLRHLIMREARAPSLVRIRANKTDAFGR PVPDAAVWVKTDGNAVSQLWRLTSWTDDGRRIPLPKPIEKRIEISNLEYARLNGLDEGAGV TGNNAPPRPLRQDIDRLTPLWRDHGTAPGGYLGTAVGELEDKARSALRGKAMRQTLTDAGIT AEAGWRLDSEGAVCDLEVAKGDTVKKDGKTYKVGVITQGIFGMPVDAAGSAPRTPEDCEKFE EQYGIKPWKAKGIPLA

SEQ ID NO: 343

MNYTEKEKLFMKYILALDIGIASVGWAILDKESETVIEAGSNIFPEASAADNQLRRDMRGAK RNNRRLKTRINDFIKLWENNNLSIPQFKSTEIVGLKVRAITEEITLDELYLILYSYLKHRGI SYLEDALDDTVSGSSAYANGLKLNAKELETHYPCEIQQERLNTIGKYRGQSQIINENGEVLD LSNVFTIGAYRKEIQRVFEIQKKYHPELTDEFCDGYMLIFNRKRKYYEGPGNEKSRTDYGRF TTKLDANGNYITEDNIFEKLIGKCSVYPDELRAAAASYTAQEYNVLNDLNNLTINGRKLEEN EKHEIVERIKSSNTINMRKIISDCMGENIDDFAGARIDKSGKEIFHKFEVYNKMRKALLEIG IDISNYSREELDEIGYIMTINTDKEAMMEAFQKSWIDLSDDVKQCLINMRKTNGALFNKWQS FSLKIMNELIPEMYAQPKEQMTLLTEMGVTKGTQEEFAGLKYIPVDVVSEDIFNPWRRSVR ISFKILNAVLKKYKALDTIVIEMPRDRNSEEQKKRINDSQKLNEKEMEYIEKKLAVTYGIKL SPSDFSSQKQLSLKLKLWNEQDGICLYSGKTIDPNDIINNPQLFEIDHIIPRSISFDDARSN KVLVYRSENQKKGNQTPYYYLTHSHSEWSFEQYKATVMNLSKKKEYAISRKKIQNLLYSEDI TKMDVLKGFINRNINDTSYASRLVLNTIQNFFMANEADTKVKVIKGSYTHQMRCNLKLDKNR DESYSHHAVDAMLIGYSELGYEAYHKLQGEFIDFETGEILRKDMWDENMSDEVYADYLYGKK WANIRNEWKAEKNVKYWHYVMRKSNRGLCNQTIRGTREYDGKQYKINKLDIRTKEGIKVFA KLAFSKKDSDRERLLVYLNDRRTFDDLCKIYEDYSDAANPFVQYEKETGDI IRKYSKKHNGP RIDKLKYKDGEVGACIDISHKYGFEKGSKKVILESLVPYRMDVYYKEENHSYYLVGVKQSDI KFEKGRNVIDEEAYARILVNEKMIQPGQSRADLENLGFKFKLSFYKNDIIEYEKDGKIYTER LVSRTMPKQRNYIETKPIDKAKFEKQNLVGLGKTKFIKKYRYDILGNKYSCSEEKFTSFC

SEQ ID NO: 344

MLRLYCANNLVLNNVQNLWKYLLLLIFDKKI IFLFKIKVILIRRYMENNNKEKIVIGFDLGV ASVGWSIVNAETKEVIDLGVRLFSEPEKADYRRAKRTTRRLLRRKKFKREKFHKLILKNAEI FGLQSRNEILNVYKDQSSKYRNILKLKINALKEEIKPSELVWILRDYLQNRGYFYKNEKLTD EFVSNSFPSKKLHEHYEKYGFFRGSVKLDNKLDNKKDKAKEKDEEEESDAKKESEELIFSNK QWINEIVKVFENQSYLTESFKEEYLKLFNYVRPFNKGPGSKNSRTAYGVFSTDIDPETNKFK DYSNIWDKTIGKCSLFEEEIRAPKNLPSALIFNLQNEICTIKNEFTEFKNWWLNAEQKSEIL KFVFTELFNWKDKKYSDKKFNKNLQDKIKKYLLNFALENFNLNEEILKNRDLENDTVLGLKG VKYYEKSNATADAALEFSSLKPLYVFIKFLKEKKLDLNYLLGLENTEILYFLDSIYLAISYS SDLKERNEWFKKLLKELYPKIKNNNLEIIENVEDIFEITDQEKFESFSKTHSLSREAFNHII PLLLSNNEGKNYESLKHSNEELKKRTEKAELKAQQNQKYLKDNFLKEALVPLSVKTSVLQAI KIFNQIIKNFGKKYEISQVVIEMARELTKPNLEKLLNNATNSNIKILKEKLDQTEKFDDFTK KKFIDKIENSVVFRNKLFLWFEQDRKDPYTQLDIKINEIEDETEIDHVIPYSKSADDSWFNK LLVKKSTNQLKKNKTVWEYYQNESDPEAKWNKFVAWAKRIYLVQKSDKESKDNSEKNSIFKN KKPNLKFKNITKKLFDPYKDLGFLARNLNDTRYATKVFRDQLNNYSKHHSKDDENKLFKWC MNGSITSFLRKSMWRKNEEQVYRFNFWKKDRDQFFHHAVDASI IAIFSLLTKTLYNKLRVYE SYDVQRREDGVYLINKETGEVKKADKDYWKDQHNFLKIRENAIEIKNVLNNVDFQNQVRYSR KANTKLNTQLFNETLYGVKEFENNFYKLEKVNLFSRKDLRKFILEDLNEESEKNKKNENGSR KRILTEKYIVDEILQILENEEFKDSKSDINALNKYMDSLPSKFSEFFSQDFINKCKKENSLI LTFDAIKHNDPKKVIKIKNLKFFREDATLKNKQAVHKDSKNQIKSFYESYKCVGFIWLKNKN DLEESIFVPINSRVIHFGDKDKDIFDFDSYNKEKLLNEINLKRPENKKFNSINEIEFVKFVK PGALLLNFENQQIYYISTLESSSLRAKIKLLNKMDKGKAVSMKKITNPDEYKIIEHVNPLGI NLNWTKKLENNN

SEQ ID NO: 345

MLMSKHVLGLDLGVGSIGWCLIALDAQGDPAEILGMGSRWPLNNATKAIEAFNAGAAFTAS QERTARRTMRRGFARYQLRRYRLRRELEKVGMLPDAALIQLPLLELWELRERAATAGRRLTL PELGRVLCHINQKRGYRHVKSDAAAIVGDEGEKKKDSNSAYLAGIRANDEKLQAEHKTVGQY FAEQLRQNQSESPTGGISYRIKDQIFSRQCYIDEYDQIMAVQRVHYPDILTDEFIRMLRDEV IFMQRPLKSCKHLVSLCEFEKQERVMRVQQDDGKGGWQLVERRVKFGPKVAPKSSPLFQLCC IYEAVNNIRLTRPNGSPCDITPEERAKIVAHLQSSASLSFAALKKLLKEKALIADQLTSKSG LKGNSTRVALASALQPYPQYHHLLDMELETRMMTVQLTDEETGEVTEREVAWTDSYVRKPL YRLWHILYSIEEREAMRRALITQLGMKEEDLDGGLLDQLYRLDFVKPGYGNKSAKFICKLLP QLQQGLGYSEACAAVGYRHSNSPTSEEITERTLLEKIPLLQRNELRQPLVEKILNQMINLVN ALKAEYGIDEVRVELARELKMSREERERMARNNKDREERNKGVAAKIRECGLYPTKPRIQKY MLWKEAGRQCLYCGRSIEEEQCLREGGMEVEHIIPKSVLYDDSYGNKTCACRRCNKEKGNRT ALEYIRAKGREAEYMKRINDLLKEKKISYSKHQRLRWLKEDIPSDFLERQLRLTQYISRQAM AILQQGIRRVSASEGGVTARLRSLWGYGKILHTLNLDRYDSMGETERVSREGEATEELHITN WSKRMDHRHHAIDALWACTRQSYIQRLNRLSSEFGREDKKKEDQEAQEQQATETGRLSNLE RWLTQRPHFSVRTVSDKVAEILISYRPGQRVVTRGR IYRKKMADGREVSCVQRGVLVPRGE LMEASFYGKILSQGRVRIVKRYPLHDLKGEVVDPHLRELITTYNQELKSREKGAPIPPLCLD KDKKQEVRSVRCYAKTLSLDKAIPMCFDEKGEPTAFVKSASNHHLALYRTPKGKLVESIVTF WDAVDRARYGIPLVITHPREVMEQVLQRGDIPEQVLSLLPPSDWVFVDSLQQDEMWIGLSD EELQRALEAQNYRKISEHLYRVQKMSSSYYVFRYHLETSVADDKNTSGRIPKFHRVQSLKAY EERNIRKVRVDLLGRISLL

SEQ ID NO: 346

MSDLVLGLDIGIGSVGVGILNKVTGEI IHK SRIFPAAQAENNLVRRTNRQGRRLARRKKHR RVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEELFIALKNMVKHRGISYLDDA SDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQLRGDFTVEKDGKKHRLINVFPT SAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKRKYYHGPGNEKSRTDYGRYRTSGETL DNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLNDLNNLTVPTETKKLSKEQKNQIINYVKN EKAMGPAKLFKYIAKLLSCDVADIKGYRIDKSGKAEIHTFEAYRKMKTLETLDIEQMDRETL DKLAYVLTLNTEREGIQEALEHEFADGSFSQKQVDELVQFRKANSSIFGKGWHNFSVKLMME LIPELYETSEEQMTILTRLGKQKTTSSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAA IKEYGDFDNIVIEMARETNEDDEKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHG HKQLATKIRLWHQQGERCLYTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYAT ANQEKGQRTPYQALDSMDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIER NLVDTRYASRVVLNALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALI IAASSQLNLWKKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFED SILFSYQVDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKD KSKFLMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGP EIKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQFEK GTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTMPKQKH YVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQHI IKNEGDK PKLDF

SEQ ID NO: 347

MNAEHGKEGLLIMEENFQYRIGLDIGITSVGWAVLQNNSQDEPVRITDLGVRIFDVAENPKN GDALAAPRRDARTTRRRLRRRRHRLERIKFLLQENGLIEMDSFMERYYKGNLPDVYQLRYEG LDRKLKDEELAQVLIHIAKHRGFRSTRKAETKEKEGGAVLKATTENQKIMQEKGYRTVGEML YLDEAFHTECLWNEKGYVLTPRNRPDDYKHTILRSMLVEEVHAIFAAQRAHGNQKATEGLEE AYVEIMTSQRSFDMGPGLQPDGKPSPYAMEGFGDRVGKCTFEKDEYRAPKATYTAELFVALQ KINHTKLIDEFGTGRFFSEEERKTIIGLLLSSKELKYGTIRKKLNIDPSLKFNSLNYSAKKE GETEEERVLDTEKAKFASMFWTYEYSKCLKDRTEEMPVGEKADLFDRIGEILTAYKNDDSRS SRLKELGLSGEEIDGLLDLSPAKYQRVSLKAMRKMQPYLEDGLIYDKACEAAGYDFRALNDG NKKHLLKGEEINAIVNDITNPWKRSVSQTIKVINAIIQKYGSPQAVNIELAREMSKNFQDR TNLEKEMKKRQQENERAKQQIIELGKQNPTGQDILKYRLWNDQGGYCLYSGKKIPLEELFDG GYDIDHILPYSITFDDSYRNKVLVTAQENRQKGNRTPYEYFGADEKRWEDYEASVRLLVRDY KKQQKLLKKNFTEEERKEFKERNLNDTKYITRWYNMIRQNLELEPFNHPEKKKQVWAVNGA VTSYLRKRWGLMQKDRSTDRHHAMDAVVIACCTDGMIHKISRYMQGRELAYSRNFKFPDEET GEILNRDNFTREQWDEKFGVKVPLPWNSFRDELDIRLLNEDPKNFLLTHADVQRELDYPGWM YGEEESPIEEGRYINYIRPLFVSRMPNHKVTGSAHDATIRSARDYETRGWITKVPLTDLKL NKDNEIEGYYDKDSDRLLYQALVRQLLLHGNDGKKAFAEDFHKPKADGTEGPWRKVKIEKK QTSGVMVRGGTGIAANGEMVRIDVFRENGKYYFVPVYTADWRKVLPNRAATHTKPYSEWRV MDDANFVFSLYSRDLIHVKSKKDIKTNLVNGGLLLQKEIFAYYTGADIATASIAGFANDSNF KFRGLGIQSLEIFEKCQVDILGNISWRHENRQEFH

SEQ ID NO: 348

MRVLGLDAGIASLGWALIEIEESNRGELSQGTIIGAGTWMFDAPEEKTQAGAKLKSEQRRTF RGQRRWRRRRQRMNEVRRILHSHGLLPSSDRDALKQPGLDPWRIRAEALDRLLGPVELAVA LGHIARHRGFKSNSKGAKTNDPADDTSKMKRAVNETREKLARFGSAAKMLVEDESFVLRQTP TKNGASEIVRRFRNREGDYSRSLLRDDLAAEMRALFTAQARFQSAIATADLQTAFTKAAFFQ RPLQDSEKLVGPCPFEVDEKRAPKRGYSFELFRFLSRLNHVTLRDGKQERTLTRDELALAAA DFGAAAKVSFTALRKKLKLPETTVFVGVKADEESKLDWARSGKAAEGTARLRSVIVDALGE LAWGALLCSPEKLDKIAEVISFRSDIGRISEGLAQAGCNAPLVDALTAAASDGRFDPFTGAG HISSKAARNILSGLRQGMTYDKACCAADYDHTASRERGAFDVGGHGREALKRILQEERISRE LVGSPTARKALIESIKQVKAIVERYGVPDRIHVELARDVGKSIEEREEITRGIEKRNRQKDK LRGLFEKEVGRPPQDGARGKEELLRFELWSEQMGRCLYTDDYISPSQLVATDDAVQVDHILP WSRFADDSYANKTLCMAKANQDKKGRTPYEWFKAEKTDTEWDAFIVRVEALADMKGFKKRNY KLRNAEEAAAKFRNRNLNDTRWACRLLAEALKQLYPKGEKDKDGKERRRVFSRPGALTDRLR RAWGLQWMKKSTKGDRIPDDRHHALDAIVIAATTESLLQRATREVQEIEDKGLHYDLVKNVT PPWPGFREQAVEAVEKVFVARAERRRARGKAHDATIRHIAVREGEQRVYERRKVAELKLADL DRVKDAERNARLIEKLRNWIEAGSPKDDPPLSPKGDPIFKVRLVTKSKVNIALDTGNPKRPG TVDRGEMARVDVFRKASKKGKYEYYLVPIYPHDIATMKTPPIRAVQAYKPEDEWPEMDSSYE FCWSLVPMTYLQVISSKGEIFEGYYRGMNRSVGAIQLSAHSNSSDWQGIGARTLTEFKKFN VDRFGRKHEVERELRTWRGETWRGKAYI

SEQ ID NO: 349

MGNYYLGLDVGIGSIGWAVINIEKKRIEDFNVRIFKSGEIQEKNRNSRASQQCRRSRGLRRL YRRKSHRKLRLKNYLSIIGLTTSEKIDYYYETADNNVIQLRNKGLSEKLTPEEIAACLIHIC NNRGYKDFYEVNVEDIEDPDERNEYKEEHDSIVLISNLMNEGGYCTPAEMICNCREFDEPNS VYRKFHNSAASKNHYLITRHMLVKEVDLILENQSKYYGILDDKTIAKIKDI IFAQRDFEIGP GKNERFRRFTGYLDSIGKCQFFKDQERGSRFTVIADIYAFVNVLSQYTYTNNRGESVFDTSF ANDLINSALKNGSMDKRELKAIAKSYHIDISDKNSDTSLTKCFKYIKWKPLFEKYGYDWDK LIENYTDTDNNVLNRIGIVLSQAQTPKRRREKLKALNIGLDDGLINELTKLKLSGTANVSYK YMQGSIEAFCEGDLYGKYQAKFNKEIPDIDENAKPQKLPPFKNEDDCEFFKNPWFRSINET RKLINAI IDKYGYPAAVNIETADELNKTFEDRAIDTKRNNDNQKENDRIVKEIIECIKCDEV HARHLIEKYKLWEAQEGKCLYSGETITKEDMLRDKDKLFEVDHIVPYSLILDNTINNKALVY AEENQKKGQRTPLMYMNEAQAADYRVRVNTMFKSKKCSKKKYQYLMLPDLNDQELLGGWRSR NLNDTRYICKYLVNYLRKNLRFDRSYESSDEDDLKIRDHYRVFPVKSRFTSMFRRWWLNEKT WGRYDKAELKKLTYLDHAADAII IANCRPEYWLAGEKLKLNKMYHQAGKRITPEYEQSKKA CIDNLYKLFRMDRRTAEKLLSGHGRLTPIIPNLSEEVDKRLWDKNIYEQFWKDDKDKKSCEE LYRENVASLYKGDPKFASSLSMPVISLKPDHKYRGTITGEEAIRVKEIDGKLIKLKRKSISE ITAESINSIYTDDKILIDSLKTIFEQADYKDVGDYLKKTNQHFFTTSSGKRVNKVTVIEKVP SRWLRKEIDDNNFSLLNDSSYYCIELYKDSKGDNNLQGIAMSDIVHDRKTKKLYLKPDFNYP DDYYTHVMYIFPGDYLRIKSTSKKSGEQLKFEGYFISVKNVNENSFRFISDNKPCAKDKRVS ITKKDIVIKLAVDLMGKVQGENNGKGISCGEPLSLLKEKN SEQ ID NO: 350

MLSRQLLGASHLARPVSYSYNVQDNDVHCSYGERCFMRGKRYRIGIDVGLNSVGLAAVEVSD ENSPVRLLNAQSVIHDGGVDPQKNKEAITRKNMSGVARRTRRMRRRKRERLHKLDMLLGKFG YPVIEPESLDKPFEEWHVRAELATRYIEDDELRRESISIALRHMARHRGWRNPYRQVDSLIS DNPYSKQYGELKEKAKAYNDDATAAEEESTPAQLVVAMLDAGYAEAPRLRWRTGSKKPDAEG YLPVRLMQEDNANELKQIFRVQRVPADEWKPLFRSVFYAVSPKGSAEQRVGQDPLAPEQARA LKASLAFQEYRIANVITNLRIKDASAELRKLTVDEKQSIYDQLVSPSSEDITWSDLCDFLGF KRSQLKGVGSLTEDGEERISSRPPRLTSVQRIYESDNKIRKPLVAWWKSASDNEHEAMIRLL SNTVDIDKVREDVAYASAIEFIDGLDDDALTKLDSVDLPSGRAAYSVETLQKLTRQMLTTDD DLHEARKTLFNVTDSWRPPADPIGEPLGNPSVDRVLKNVNRYLMNCQQRWGNPVSVNIEHVR SSFSSVAFARKDKREYEKNNEKRSIFRSSLSEQLRADEQMEKVRESDLRRLEAIQRQNGQCL YCGRTITFRTCEMDHIVPRKGVGSTNTRTNFAAVCAECNRMKSNTPFAIWARSEDAQTRGVS LAEAKKRVTMFTFNPKSYAPREVKAFKQAVIARLQQTEDDAAIDNRSIESVAWMADELHRRI DWYFNAKQYVNSASIDDAEAETMKTTVSVFQGRVTASARRAAGIEGKIHFIGQQSKTRLDRR HHAVDASVIAMMNTAAAQTLMERESLRESQRLIGLMPGERSWKEYPYEGTSRYESFHLWLDN MDVLLELLNDALDNDRIAVMQSQRYVLGNSIAHDATIHPLEKVPLGSAMSADLIRRASTPAL WCALTRLPDYDEKEGLPEDSHREIRVHDTRYSADDEMGFFASQAAQIAVQEGSADIGSAIHH ARVYRCWKTNAKGVRKYFYGMIRVFQTDLLRACHDDLFTVPLPPQSISMRYGEPRWQALQS GNAQYLGSLWGDEIEMDFSSLDVDGQIGEYLQFFSQFSGGNLAWKHWWDGFFNQTQLRIR PRYLAAEGLAKAFSDDWPDGVQKIVTKQGWLPPVNTASKTAVRIVRRNAFGEPRLSSAHHM PCSWQWRHE

SEQ ID NO: 351

MYSIGLDLGISSVGWSVIDERTGNVIDLGVRLFSAKNSEKNLERRTNRGGRRLIRRKTNRLK DAKKILAAVGFYEDKSLKNSCPYQLRVKGLTEPLSRGEIYKVTLHILKKRGISYLDEVDTEA AKESQDYKEQVRKNAQLLTKYTPGQIQLQRLKENNRVKTGINAQGNYQLNVFKVSAYANELA TILKTQQAFYPNELTDDWIALFVQPGIAEEAGLIYRKRPYYHGPGNEANNSPYGRWSDFQKT GEPATNIFDKLIGKDFQGELRASGLSLSAQQYNLLNDLTNLKIDGEVPLSSEQKEYILTELM TKEFTRFGVNDWKLLGVKKERLSGWRLDKKGKPEIHTLKGYRNWRKIFAEAGIDLATLPTE TIDCLAKVLTLNTEREGIENTLAFELPELSESVKLLVLDRYKELSQSISTQSWHRFSLKTLH LLIPELMNATSEQNTLLEQFQLKSDVRKRYSEYKKLPTKDVLAEIYNPTVNKTVSQAFKVID ALLVKYGKEQIRYITIEMPRDDNEEDEKKRIKELHAKNSQRKNDSQSYFMQKSGWSQEKFQT TIQKNRRFLAKLLYYYEQDGICAYTGLPISPELLVSDSTEIDHIIPISISLDDSINNKVLVL SKANQVKGQQTPYDAWMDGSFKKINGKFSNWDDYQKWVESRHFSHKKENNLLETRNIFDSEQ VEKFLARNLNDTRYASRLVLNTLQSFFTNQETKVRWNGSFTHTLRKKWGADLDKTRETHHH HAVDATLCAVTSFVKVSRYHYAVKEETGEKVMREIDFETGEIVNEMSYWEFKKSKKYERKTY QVKWPNFREQLKPVNLHPRIKFSHQVDRKANRKLSDATIYSVREKTEVKTLKSGKQKITTDE YTIGKIKDIYTLDGWEAFKKKQDKLLMKDLDEKTYERLLSIAETTPDFQEVEEKNGKVKRVK RSPFAVYCEENDIPAIQKYAKKNNGPLIRSLKYYDGKLNKHINITKDSQGRPVEKTKNGRKV TLQSLKPYRYDIYQDLETKAYYTVQLYYSDLRFVEGKYGITEKEYMKKVAEQTKGQWRFCF SLQKNDGLEIEWKDSQRYDVRFYNFQSANSINFKGLEQEMMPAENQFKQKPYNNGAINLNIA KYGKEGKKLRKFNTDILGKKHYLFYEKEPKNIIK

SEQ ID NO: 352

MYFYKNKENKLNKKVVLGLDLGIASVGWCLTDISQKEDNKFPI ILHGVRLFETVDDSDDKLL NETRRKKRGQRRRNRRLFTRKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLE LKYKSVTNLPIGFHNLRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNE IEIFDKNESIKNAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYE KFAMEEKSFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESK IGKCSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAVE KILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNENDLSKLM SFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEGYFEFSNLKK DVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYEEKALQNQKNNFSH SNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEKIVVEVTRSSNNKHERKK IEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGYDAYSLRKIEANDVINKPWNYD IDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQFIEKIYGIEKLKEAKENWGNWYLRN ANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRNLSDTSYITNALVNHLTFSNSKYKYSVVSV NGKQTSNLRNQIAFVGIKNNKETEREWKRPEGFKSINSNDFLIREEGKNDVKDDVLIKDRSF NGHHAEDAYFITIISQYFRSFKRIERLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINA LDELNEKLNQMRFSRMVITKKNTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIE EFFDEDKLKENELTKLHIFNHDKNLYETLKI IWNEVKIEIKNKNLNEKNYFKYFVNKKLQEG KISFNEWVPILDNDFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQ IWVYKNQKDQYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENE EKELFYIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK

SEQ ID NO: 353

MDNKNYRIGIDVGLNSIGFCAVEVDQHDTPLGFLNLSVYRHDAGIDPNGKKTNTTRLAMSGV ARRTRRLFRKRKRRLAALDRFIEAQGWTLPDHADYKDPYTPWLVRAELAQTPIRDENDLHEK LAIAVRHIARHRGWRSPWVPVRSLHVEQPPSDQYLALKERVEAKTLLQMPEGATPAEMVVAL DLSVDVNLRPKNREKTDTRPENKKPGFLGGKLMQSDNANELRKIAKIQGLDDALLRELIELV FAADSPKGASGELVGYDVLPGQHGKRRAEKAHPAFQRYRIASIVSNLRIRHLGSGADERLDV ETQKRVFEYLLNAKPTADITWSDVAEEIGVERNLLMGTATQTADGERASAKPPVDVTNVAFA TCKIKPLKEWWLNADYEARCVMVSALSHAEKLTEGTAAEVEVAEFLQNLSDEDNEKLDSFSL PIGRAAYSVDSLERLTKRMIENGEDLFEARVNEFGVSEDWRPPAEPIGARVGNPAVDRVLKA VNRYLMAAEAEWGAPLSVNIEHVREGFISKRQAVEIDRENQKRYQRNQAVRSQIADHINATS GVRGSDVTRYLAIQRQNGECLYCGTAITFVNSEMDHIVPRAGLGSTNTRDNLVATCERCNKS KSNKPFAVWAAECGIPGVSVAEALKRVDFWIADGFASSKEHRELQKGVKDRLKRKVSDPEID NRSMESVAWMARELAHRVQYYFDEKHTGTKVRVFRGSLTSAARKASGFESRVNFIGGNGKTR LDRRHHAMDAATVAMLRNSVAKTLVLRGNIRASERAIGAAETWKSFRGENVADRQIFESWSE NMRVLVEKFNLALYNDEVSIFSSLRLQLGNGKAHDDTITKLQMHKVGDAWSLTEIDRASTPA LWCALTRQPDFTWKDGLPANEDRTIIVNGTHYGPLDKVGIFGKAAASLLVRGGSVDIGSAIH HARIYRIAGKKPTYGMVRVFAPDLLRYRNEDLFNVELPPQSVSMRYAEPKVREAIREGKAEY LGWLWGDELLLDLSSETSGQIAELQQDFPGTTHWTVAGFFSPSRLRLRPVYLAQEGLGEDV SEGSKSI IAGQGWRPAVNKVFGSAMPEVIRRDGLGRKRRFSYSGLPVSWQG

SEQ ID NO: 354

MRLGLDIGTSSIGWWLYETDGAGSDARITGVVDGGVRIFSDGRDPKSGASLAVDRRAARAMR RRRDRYLRRRATLMKVLAETGLMPADPAEAKALEALDPFALRAAGLDEPLPLPHLGRALFHL NQRRGFKSNRKTDRGDNESGKIKDATARLDMEMMANGARTYGEFLHKRRQKATDPRHVPSVR TRLSIANRGGPDGKEEAGYDFYPDRRHLEEEFHKLWAAQGAHHPELTETLRDLLFEKIFFQR PLKEPEVGLCLFSGHHGVPPKDPRLPKAHPLTQRRVLYETVNQLRVTADGREARPLTREERD QVIHALDNKKPTKSLSSMVLKLPALAKVLKLRDGERFTLETGVRDAIACDPLRASPAHPDRF GPRWSILDADAQWEVISRIRRVQSDAEHAALVDWLTEAHGLDRAHAEATAHAPLPDGYGRLG LTATTRILYQLTADVVTYADAVKACGWHHSDGRTGECFDRLPYYGEVLERHVIPGSYHPDDD DITRFGRITNPTVHIGLNQLRRLVNRI IETHGKPHQIWELARDLKKSEEQKRADIKRIRDT TEAAKKRSEKLEELEIEDNGRNRMLLRLWEDLNPDDAMRRFCPYTGTRISAAMIFDGSCDVD HILPYSRTLDDSFPNRTLCLREANRQKRNQTPWQAWGDTPHWHAIAANLKNLPENKRWRFAP DAMTRFEGENGFLDRALKDTQYLARISRSYLDTLFTKGGHVWVVPGRFTEMLRRHWGLNSLL SDAGRGAVKAKNRTDHRHHAIDAAVIAATDPGLLNRISRAAGQGEAAGQSAELIARDTPPPW EGFRDDLRVRLDRIIVSHRADHGRIDHAARKQGRDSTAGQLHQETAYSIVDDIHVASRTDLL SLKPAQLLDEPGRSGQVRDPQLRKALRVATGGKTGKDFENALRYFASKPGPYQAIRRVRIIK PLQAQARVPVPAQDPIKAYQGGSNHLFEIWRLPDGEIEAQVITSFEAHTLEGEKRPHPAAKR LLRVHKGDMVALERDGRRVVGHVQKMDIANGLFIVPHNEANADTRNNDKSDPFKWIQIGARP AIASGIRRVSVDEIGRLRDGGTRPI

SEQ ID NO: 355

MLHCIAVIRVPPSEEPGFFETHADSCALCHHGCMTYAANDKAIRYRVGIDVGLRSIGFCAVE VDDEDHPIRILNSWHVHDAGTGGPGETESLRKRSGVAARARRRGRAEKQRLKKLDVLLEEL GWGVSSNELLDSHAPWHIRKRLVSEYIEDETERRQCLSVAMAHIARHRGWRNSFSKVDTLLL EQAPSDRMQGLKERVEDRTGLQFSEEVTQGELVATLLEHDGDVTIRGFVRKGGKATKVHGVL EGKYMQSDLVAELRQICRTQRVSETTFEKLVLSIFHSKEPAPSAARQRERVGLDELQLALDP AAKQPRAERAHPAFQKFKVVATLANMRIREQSAGERSLTSEELNRVARYLLNHTESESPTWD DVARKLEVPRHRLRGSSRASLETGGGLTYPPVDDTTVRVMSAEVDWLADWWDCANDESRGHM IDAISNGCGSEPDDVEDEEVNELISSATAEDMLKLELLAKKLPSGRVAYSLKTLREVTAAIL ETGDDLSQAITRLYGVDPGWVPTPAPIEAPVGNPSVDRVLKQVARWLKFASKRWGVPQTVNI EHTREGLKSASLLEEERERWERFEARREIRQKEMYKRLGISGPFRRSDQVRYEILDLQDCAC LYCGNEINFQTFEVDHIIPRVDASSDSRRTNLAAVCHSCNSAKGGLAFGQWVKRGDCPSGVS LENAIKRVRSWSKDRLGLTEKAMGKRKSEVISRLKTEMPYEEFDGRSMESVAWMAIELKKRI EGYFNSDRPEGCAAVQVNAYSGRLTACARRAAHVDKRVRLIRLKGDDGHHKNRFDRRNHAMD ALVIALMTPAIARTIAVREDRREAQQLTRAFESWKNFLGSEERMQDRWESWIGDVEYACDRL NELIDADKIPVTENLRLRNSGKLHADQPESLKKARRGSKRPRPQRYVLGDALPADVINRVTD PGLWTALVRAPGFDSQLGLPADLNRGLKLRGKRISADFPIDYFPTDSPALAVQGGYVGLEFH HARLYRI IGPKEKVKYALLRVCAIDLCGIDCDDLFEVELKPSSISMRTADAKLKEAMGNGSA KQIGWLVLGDEIQIDPTKFPKQSIGKFLKECGPVSSWRVSALDTPSKITLKPRLLSNEPLLK TSRVGGHESDLWAECVEKIMKKTGWVVEINALCQSGLIRVIRRNALGEVRTSPKSGLPISL NLR

SEQ ID NO: 356

MRYRVGLDLGTASVGAAVFSMDEQGNPMELIWHYERLFSEPLVPDMGQLKPKKAARRLARQQ RRQIDRRASRLRRIAIVSRRLGIAPGRNDSGVHGNDVPTLRAMAVNERIELGQLRAVLLRMG KKRGYGGTFKAVRKVGEAGEVASGASRLEEEMVALASVQNKDSVTVGEYLAARVEHGLPSKL KVAANNEYYAPEYALFRQYLGLPAIKGRPDCLPNMYALRHQIEHEFERIWATQSQFHDVMKD HGVKEEIRNAIFFQRPLKSPADKVGRCSLQTNLPRAPRAQIAAQNFRIEKQMADLRWGMGRR AEMLNDHQKAVIRELLNQQKELSFRKIYKELERAGCPGPEGKGLNMDRAALGGRDDLSGNTT LAAWRKLGLEDRWQELDEVTQIQVINFLADLGSPEQLDTDDWSCRFMGKNGRPRNFSDEFVA FMNELRMTDGFDRLSKMGFEGGRSSYSIKALKALTEWMIAPHWRETPETHRVDEEAAIRECY PESLATPAQGGRQSKLEPPPLTGNEWDVALRQVRHTINMMIDDLGSVPAQIWEMAREMKG GVTRRNDIEKQNKRFASERKKAAQSIEENGKTPTPARILRYQLWIEQGHQCPYCESNISLEQ ALSGAYTNFEHILPRTLTQIGRKRSELVLAHRECNDEKGNRTPYQAFGHDDRRWRIVEQRAN ALPKKSSRKTRLLLLKDFEGEALTDESIDEFADRQLHESSWLAKVTTQWLSSLGSDVYVSRG SLTAELRRRWGLDTVIPQVRFESGMPVVDEEGAEITPEEFEKFRLQWEGHRVTREMRTDRRP DKRIDHRHHLVDAIVTALTSRSLYQQYAKAWKVADEKQRHGRVDVKVELPMPILTIRDIALE AVRSVRISHKPDRYPDGRFFEATAYGIAQRLDERSGEKVDWLVSRKSLTDLAPEKKSIDVDK VRANISRIVGEAIRLHISNIFEKRVSKGMTPQQALREPIEFQGNILRKVRCFYSKADDCVRI EHSSRRGHHYKMLLNDGFAYMEVPCKEGILYGVPNLVRPSEAVGIKRAPESGDFIRFYKGDT VKNIKTGRVYTIKQILGDGGGKLILTPVTETKPADLLSAKWGRLKVGGRNIHLLRLCAE

SEQ ID NO: 357

MIGEHVRGGCLFDDHWTPNWGAFRLPNTVRTFTKAENPKDGSSLAEPRRQARGLRRRLRRKT QRLEDLRRLLAKEGVLSLSDLETLFRETPAKDPYQLRAEGLDRPLSFPEWVRVLYHITKHRG FQSNRRNPVEDGQERSRQEEEGKLLSGVGENERLLREGGYRTAGEMLARDPKFQDHRRNRAG DYSHTLSRSLLLEEARRLFQSQRTLGNPHASSNLEEAFLHLVAFQNPFASGEDIRNKAGHCS LEPDQIRAPRRSASAETFMLLQKTGNLRLIHRRTGEERPLTDKEREQIHLLAWKQEKVTHKT LRRHLEIPEEWLFTGLPYHRSGDKAEEKLFVHLAGIHEIRKALDKGPDPAVWDTLRSRRDLL DSIADTLTFYKNEDEILPRLESLGLSPENARALAPLSFSGTAHLSLSALGKLLPHLEEGKSY TQARADAGYAAPPPDRHPKLPPLEEADWRNPWFRALTQTRKVVNALVRRYGPPWCIHLETA RELSQPAKVRRRIETEQQANEKKKQQAEREFLDIVGTAPGPGDLLKMRLWREQGGFCPYCEE YLNPTRLAEPGYAEMDHILPYSRSLDNGWHNRVLVHGKDNRDKGNRTPFEAFGGDTARWDRL VAWVQASHLSAPKKRNLLREDFGEEAERELKDRNLTDTRFITKTAATLLRDRLTFHPEAPKD PVMTLNGRLTAFLRKQWGLHKNRKNGDLHHALDAAVLAVASRSFVYRLSSHNAAWGELPRGR EAENGFSLPYPAFRSEVLARLCPTREEILLRLDQGGVGYDEAFRNGLRPVFVSRAPSRRLRG KAHMETLRSPKWKDHPEGPRTASRIPLKDLNLEKLERMVGKDRDRKLYEALRERLAAFGGNG KKAFVAPFRKPCRSGEGPLVRSLRIFDSGYSGVELRDGGEVYAVADHESMVRVDVYAKKNRF YLVPVYVADVARGIVKNRAIVAHKSEEEWDLVDGSFDFRFSLFPGDLVEIEKKDGAYLGYYK SCHRGDGRLLLDRHDRMPRESDCGTFYVSTRKDVLSMSKYQVDPLGEIRLVGSEKPPFVL

SEQ ID NO: 358

MEKKRKVTLGFDLGIASVGWAIVDSETNQVYKLGSRLFDAPDTNLERRTQRGTRRLLRRRKY RNQKFYNLVKRTEVFGLSSREAIENRFRELSIKYPNIIELKTKALSQEVCPDEIAWILHDYL KNRGYFYDEKETKEDFDQQTVESMPSYKLNEFYKKYGYFKGALSQPTESEMKDNKDLKEAFF FDFSNKEWLKEINYFFNVQKNILSETFIEEFKKIFSFTRDISKGPGSDNMPSPYGIFGEFGD NGQGGRYEHIWDKNIGKCSIFTNEQRAPKYLPSALIFNFLNELANIRLYSTDKKNIQPLWKL SSVDKLNILLNLFNLPISEKKKKLTSTNINDIVKKESIKSIMISVEDIDMIKDEWAGKEPNV YGVGLSGLNIEESAKENKFKFQDLKILNVLINLLDNVGIKFEFKDRNDIIKNLELLDNLYLF LIYQKESNNKDSSIDLFIAKNESLNIENLKLKLKEFLLGAGNEFENHNSKTHSLSKKAIDEI LPKLLDNNEGWNLEAIKNYDEEIKSQIEDNSSLMAKQDKKYLNDNFLKDAILPPNVKVTFQQ AILIFNKIIQKFSKDFEIDKWIELAREMTQDQENDALKGIAKAQKSKKSLVEERLEANNID KSVFNDKYEKLIYKIFLWISQDFKDPYTGAQISVNEIVNNKVEIDHI IPYSLCFDDSSANKV LVHKQSNQEKSNSLPYEYIKQGHSGWNWDEFTKYVKRVFVNNVDSILSKKERLKKSENLLTA SYDGYDKLGFLARNLNDTRYATILFRDQLNNYAEHHLIDNKKMFKVIAMNGAVTSFIRKNMS YDNKLRLKDRSDFSHHAYDAAIIALFSNKTKTLYNLIDPSLNGIISKRSEGYWVIEDRYTGE IKELKKEDWTSIKNNVQARKIAKEIEEYLIDLDDEVFFSRKTKRKTNRQLYNETIYGIATKT DEDGITNYYKKEKFSILDDKDIYLRLLREREKFVINQSNPEVIDQIIEIIESYGKENNIPSR DEAINIKYTKNKINYNLYLKQYMRSLTKSLDQFSEEFINQMIANKTFVLYNPTKNTTRKIKF LRLVNDVKINDIRKNQVINKFNGKNNEPKAFYE I SLGAIVFKNSANNFKTLSI TQIAIF GDKNWDIEDFKTYNMEKIEKYKEIYGIDKTYNFHSFIFPGTILLDKQNKEFYYISSIQTVRD IIEIKFLNKIEFKDENKNQDTSKTPKRLMFGIKSIMNNYEQVDISPFGINKKIFE

SEQ ID NO: 359

MGYRIGLDVGITSTGYAVLKTDKNGLPYKILTLDSVIYPRAENPQTGASLAEPRRIKRGLRR RTRRTKFRKQRTQQLFIHSGLLSKPEIEQILATPQAKYSVYELRVAGLDRRLTNSELFRVLY FFIGHRGFKSNRKAELNPENEADKKQMGQLLNSIEEIRKAIAEKGYRTVGELYLKDPKYNDH KRNKGYIDGYLSTPNRQMLVDEIKQILDKQRELGNEKLTDEFYATYLLGDENRAGIFQAQRD FDEGPGAGPYAGDQIKKMVGKDIFEPTEDRAAKATYTFQYFNLLQKMTSLNYQNTTGDTWHT LNGLDRQAIIDAVFAKAEKPTKTYKPTDFGELRKLLKLPDDARFNLVNYGSLQTQKEIETVE KKTRFVDFKAYHDLVKVLPEEMWQSRQLLDHIGTALTLYSSDKRRRRYFAEELNLPAELIEK LLPLNFSKFGHLSIKSMQNIIPYLEMGQVYSEATTNTGYDFRKKQISKDTIREEITNPVVRR AVTKTIKIVEQIIRRYGKPDGINIELARELGRNFKERGDIQKRQDKNRQTNDKIAAELTELG IPVNGQNIIRYKLHKEQNGVDPYTGDQIPFERAFSEGYEVDHI IPYSISWDDSYTNKVLTSA KCNREKGNRIPMVYLANNEQRLNALTNIADNIIRNSRKRQKLLKQKLSDEELKDWKQRNIND TRFITRVLYNYFRQAIEFNPELEKKQRVLPLNGEVTSKIRSRWGFLKVREDGDLHHAIDATV IAAITPKFIQQVTKYSQHQEVKNNQALWHDAEIKDAEYAAEAQRMDADLFNKIFNGFPLPWP EFLDELLARISDNPVEMMKSRSWNTYTPIEIAKLKPVFVVRLANHKISGPAHLDTIRSAKLF DEKGIVLSRVSITKLKINKKGQVATGDGIYDPENSNNGDKWYSAIRQALEAHNGSGELAFP DGYLEYVDHGTKKLVRKVRVAKKVSLPVRLKNKAAADNGSMVRIDVFNTGKKFVFVPIYIKD TVEQVLPNKAIARGKSLWYQITESDQFCFSLYPGDMVHIESKTGIKPKYSNKENNTSWPIK NFYGYFDGADIATASILVRAHDSSYTARSIGIAGLLKFEKYQVDYFGRYHKVHEKKRQLFVK RDE SEQ ID NO: 360 MQKNINTKQNHIYIKQAQKIKEKLGDKPYRIGLDLGVGSIGFAIVSMEENDGNVLLPKEIIM VGSRIFKASAGAADRKLSRGQRNNHRHTRERMRYLWKVLAEQKLALPVPADLDRKENSSEGE TSAKRFLGDVLQKDIYELRVKSLDERLSLQELGYVLYHIAGHRGSSAIRTFENDSEEAQKEN TENKKIAGNIKRLMAKKNYRTYGEYLYKEFFENKEKHKREKISNAANNHKFSPTRDLVIKEA EAILKKQAGKDGFHKELTEEYIEKLTKAIGYESEKLIPESGFCPYLKDEKRLPASHKLNEER RLWETLNNARYSDPIVDIVTGEITGYYEKQFTKEQKQKLFDYLLTGSELTPAQTKKLLGLKN TNFEDIILQGRDKKAQKIKGYKLIKLESMPFWARLSEAQQDSFLYDWNSCPDEKLLTEKLSN EYHLTEEEIDNAFNEIVLSSSYAPLGKSAMLIILEKIKNDLSYTEAVEEALKEGKLTKEKQA IKDRLPYYGAVLQESTQKI IAKGFSPQFKDKGYKTPHTNKYELEYGRIANPWHQTLNELRK LVNEIIDILGKKPCEIGLETARELKKSAEDRSKLSREQNDNESNRNRIYEIYIRPQQQVIIT RRENPRNYILKFELLEEQKSQCPFCGGQISPNDIINNQADIEHLFPIAESEDNGRNNLVISH SACNADKAKRSPWAAFASAAKDSKYDYNRILSNVKENIPHKAWRFNQGAFEKFIENKPMAAR FKTDNSYISKVAHKYLACLFEKPNIICVKGSLTAQLRMAWGLQGLMIPFAKQLITEKESESF NKDVNSNKKIRLDNRHHALDAIVIAYASRGYGNLLNKMAGKDYKINYSERNWLSKILLPPNN IVWENIDADLESFESSVKTALKNAFISVKHDHSDNGELVKGTMYKIFYSERGYTLTTYKKLS ALKLTDPQKKKTPKDFLETALLKFKGRESEMKNEKIKSAIENNKRLFDVIQDNLEKAKKLLE EENEKSKAEGKKEKNINDASIYQKAISLSGDKYVQLSKKEPGKFFAISKPTPTTTGYGYDTG DSLCVDLYYDNKGKLCGEI IRKIDAQQKNPLKYKEQGFTLFERIYGGDILEVDFDIHSDKNS FRNNTGSAPENRVFIKVGTFTEITNN IQIWFGNI IKSTGGQDDSFTINSMQQYNPRKLILS SCGFIKYRSPILKNKEG

SEQ ID NO: 361

MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVRVFERAEVPKTGDSLAMAR RLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIKSLPNTPWQLRAAALDRKLTP LEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVADNAHALQTGDFRTPAELALNKFE KESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSG DAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERATLMDEPY RKSKLTYAQARKLLGLEDTAFFKGLRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPL NLSPELQDEIGTAFSLFKTDEDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPL MEQGKRYDEACAEIYGDHYGKKNTEEKIYLPPIPADEIRNPWLRALSQARKVINGWRRYG SPARIHIETAREVGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRL YEQQHGKCLYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPY EYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAWVACSTVAMQQKITRF VRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKL RTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLK LKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAWQGKDEEDWQLID DSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEGIGVKT ALSFQKYQIDELGKEIRPCRLKKRPPVR

SEQ ID NO: 362

MQTTNLSYILGLDLGIASVGWAVVEINENEDPIGLIDVGVRIFERAEVPKTGESLALSRRLA RSTRRLIRRRAHRLLLAKRFLKREGILSTIDLEKGLPNQAWELRVAGLERRLSAIEWGAVLL HLIKHRGYLSKRKNESQTNNKELGALLSGVAQNHQLLQSDDYRTPAELALKKFAKEEGHIRN QRGAYTHTFNRLDLLAELNLLFAQQHQFGNPHCKEHIQQYMTELLMWQKPALSGEAILKMLG KCTHEKNEFKAAKHTYSAERFVWLTKLNNLRILEDGAERALNEEERQLLINHPYEKSKLTYA QVRKLLGLSEQAIFKHLRYSKENAESATFMELKAWHAIRKALENQGLKDTWQDLAKKPDLLD EIGTAFSLYKTDEDIQQYLTNKVPNSVINALLVSLNFDKFIELSLKSLRKILPLMEQGKRYD QACREIYGHHYGEANQKTSQLLPAIPAQEIRNPWLRTLSQARKVINAIIRQYGSPARVHIE TGRELGKSFKERREIQKQQEDNRTKRESAVQKFKELFSDFSSEPKSKDILKFRLYEQQHGKC LYSGKEINIHRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLASENQNKGNQTPYEWLQGKIN SERWKNFVALVLGSQCSAAKKQRLLTQVIDDNKFIDRNLNDTRYIARFLSNYIQENLLLVGK NKKNVFTPNGQITALLRSRWGLIKARENNNRHHALDAIVVACATPSMQQKITRFIRFKEVHP YKIENRYEMVDQESGEIISPHFPEPWAYFRQEVNIRVFDNHPDTVLKEMLPDRPQANHQFVQ PLFVSRAPTRKMSGQGHMETIKSAKRLAEGISVLRIPLTQLKPNLLENMVNKEREPALYAGL KARLAEFNQDPAKAFATPFYKQGGQQVKAIRVEQVQKSGVLVRENNGVADNASIVRTDVFIK NNKFFLVPIYTWQVAKGILPNKAIVAHKNEDEWEEMDEGAKFKFSLFPNDLVELKTKKEYFF GYYIGLDRATGNISLKEHDGEISKGKDGVYRVGVKLALSFEKYQVDELGKNRQICRPQQRQP VR

SEQ ID NO: 363

MGIRFAFDLGTNSIGWAVWRTGPGVFGEDTAASLDGSGVLIFKDGRNPKDGQSLATMRRVPR QSRKRRDRFVLRRRDLLAALRKAGLFPVDVEEGRRLAATDPYHLRAKALDESLTPHEMGRVI FHLNQRRGFRSNRKADRQDREKGKIAEGSKRLAETLAATNCRTLGEFLWSRHRGTPRTRSPT RIRMEGEGAKALYAFYPTREMVRAEFERLWTAQSRFAPDLLTPERHEEIAGILFRQRDLAPP KIGCCTFEPSERRLPRALPSVEARGIYERLAHLRITTGPVSDRGLTRPERDVLASALLAGKS LTFKAVRKTLKILPHALVNFEEAGEKGLDGALTAKLLSKPDHYGAAWHGLSFAEKDTFVGKL LDEADEERLIRRLVTENRLSEDAARRCASIPLADGYGRLGRTANTEILAALVEETDETGTW TYAEAVRRAGERTGRNWHHSDERDGVILDRLPYYGEILQRHWPGSGEPEEKNEAARWGRLA NPTVHIGLNQLRKWNRLIAAHGRPDQIWELARELKLNREQKERLDRENRKNREENERRTA ILAEHGQRDTAENKIRLRLFEEQARANAGIALCPYTGRAIGIAELFTSEVEIDHILPVSLTL DDSLANRVLCRREA REKRRQTPFQAFGATPAWNDIVARAAKLPPNKRWRFDPAALERFERE GGFLGRQLNETKYLSRLAKIYLGKICDPDRVYVTPGTLTGLLRARWGLNSILSDSNFKNRSD HRHHAVDAWIGVLTRGMIQRIAHDAARAEDQDLDRVFRDVPVPFEDFRDHVRERVSTITVA VKPEHGKGGALHEDTSYGLVPDTDPNAALGNLWRKPIRSLTAGEVDRVRDRALRARLGALA APFRDESGRVRDAKGLAQALEAFGAENGIRRVRILKPDASWTIADRRTGVPYRAVAPGENH HVDIVQMRDGSWRGFAASVFEVNRPGWRPEWEVKKLGGKLVMRLHKGDMVELSDKDGQRRVK WQQIEISANRVRLSPHNDGGKLQDRHADADDPFRWDLATIPLLKDRGCVAVRVDPIGVVTL RRSNV

SEQ ID NO: 364

MMEVFMGRLVLGLDIGITSVGFGIIDLDESEIVDYGVRLFKEGTAAENETRRTKRGGRRLKR RRVTRREDMLHLLKQAGIISTSFHPLNNPYDVRVKGLNERLNGEELATALLHLCKHRGSSVE TIEDDEAKAKEAGETKKVLSMNDQLLKSGKYVCEIQKERLRTNGHIRGHENNFKTRAYVDEA FQILSHQDLSNELKSAIITIISRKRMYYDGPGGPLSPTPYGRYTYFGQKEPIDLIEKMRGKC SLFPNEPRAPKLAYSAELFNLLNDLNNLSIEGEKLTSEQKAMILKIVHEKGKITPKQLAKEV GVSLEQIRGFRIDTKGSPLLSELTGYKMIREVLEKSNDEHLEDHVFYDEIAEILTKTKDIEG RKKQISELSSDLNEESVHQLAGLTKFTAYHSLSFKALRLINEEMLKTELNQMQSITLFGLKQ NNELSVKGMKNIQADDTAILSPVAKRAQRETFKWNRLREIYGEFDSIWEMAREKNSEEQR KAIRERQKFFEMRNKQVADIIGDDRKINAKLREKLVLYQEQDGKTAYSLEPIDLKLLIDDPN AYEVDHI IPISISLDDSITNKVLVTHRENQEKGNLTPISAFVKGRFTKGSLAQYKAYCLKLK EKNIKTNKGYRKKVEQYLLNENDIYKYDIQKEFINRNLVDTSYASRVVLNTLTTYFKQNEIP TKVFTVKGSLTNAFRRKINLKKDRDEDYGHHAIDALIIASMPKMRLLSTIFSRYKIEDIYDE STGEVFSSGDDSMYYDDRYFAFIASLKAIKVRKFSHKIDTKPNRSVADETIYSTRVIDGKEK WKKYKDIYDPKFTALAEDILNNAYQEKYLMALHDPQTFDQIVKWNYYFEEMSKSEKYFTK DKKGRIKISGMNPLSLYRDEHGMLKKYSKKGDGPAITQMKYFDGVLGNHIDISAHYQVRDKK WLQQISPYRTDFYYSKENGYKFVTIRYKDVRWSEKKKKYVIDQQDYAMKKAEKKIDDTYEF QFSMHRDELIGITKAEGEALIYPDETWHNFNFFFHAGETPEILKFTATNNDKSNKIEVKPIH CYCKMRLMPTISKKIVRIDKYATDWGNLYKVKKNTLKFEFD

SEQ ID NO: 365 MKKILGVDLGITSFGYAILQETGKDLYRCLDNSWMRNNPYDEKSGESSQSIRSTQKSMRRL IEKRKKRIRCVAQTMERYGILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIF AHMAKHRGYKSIATEDLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGET IAQTIHRAVEAGDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDE LKACITDQEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDRE KVIEWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLAD IAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDRELLELFK NKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLHLREGNLFEK EENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRKEIDKAMREREKAL DKI IGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLLDGSADIEHIVPQSLGGL STDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSEKGLIDWKKRKNLLAQSLDEIY TENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKNGIGVRMIPGKVTSKTRSLLGIKSKS RETNFHHAEDALILSTLTRGWQNRLHRMLRDNYGKSEAELKELWKKYMPHIEGLTLADYIDE AFRRFMSKGEESLFYRDMFDTIRSISYWVDKKPLSASSHKETVYSSRHEVPTLRKNILEAFD SLNVIKDRHKLTTEEFMKRYDKEIRQKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMD AQNDKEIDEKFQQALKELITSPIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHIS KGPNEKLIFRRMDVNNAHELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYI ALFNPRFPANPKAQPSKFTSDSKIKQVGIGSATGI IKAHLDLDGHVRSYEVFGTLPEGSIEW FKEESGYGRVEDDPHH

SEQ ID NO: 366

MRPIEPWILGLDIGTDSLGWAVFSCEEKGPPTAKELLGGGVRLFDSGRDAKDHTSRQAERGA FRRARRQTRTWPWRRDRLIALFQAAGLTPPAAETRQIALALRREAVSRPLAPDALWAALLHL AHHRGFRSNRIDKRERAAAKALAKAKPAKATAKATAPAKEADDEAGFWEGAEAALRQRMAAS GAPTVGALLADDLDRGQPVRMRYNQSDRDGVVAPTRALIAEELAEIVARQSSAYPGLDWPAV TRLVLDQRPLRSKGAGPCAFLPGEDRALRALPTVQDFIIRQTLANLRLPSTSADEPRPLTDE EHAKALALLSTARFVEWPALRRALGLKRGVKFTAETERNGAKQAARGTAGNLTEAILAPLIP GWSGWDLDRKDRVFSDLWAARQDRSALLALIGDPRGPTRVTEDETAEAVADAIQIVLPTGRA SLSAKAARAIAQAMAPGIGYDEAVTLALGLHHSHRPRQERLARLPYYAAALPDVGLDGDPVG PPPAEDDGAAAEAYYGRIGNISVHIALNETRKIVNALLHRHGPILRLVMVETTRELKAGADE RKRMIAEQAERERE AEIDVELRKSDRWMA ARERRQRVRLARRQNNLCPYTSTPIGHADLL GDAYDIDHVIPLARGGRDSLDNMVLCQSDANKTKGDKTPWEAFHDKPGWIAQRDDFLARLDP QTAKALAWRFADDAGERVARKSAEDEDQGFLPRQLTDTGYIARVALRYLSLVTNEPNAVVAT NGRLTGLLRLAWDITPGPAPRDLLPTPRDALRDDTAARRFLDGLTPPPLAKAVEGAVQARLA ALGRSRVADAGLADALGLTLASLGGGGKNRADHRHHFIDAAMIAVTTRGLINQINQASGAGR ILDLRKWPRTNFEPPYPTFRAEVMKQWDHIHPSIRPAHRDGGSLHAATVFGVRNRPDARVLV QRKPVEKLFLDANAKPLPADKIAEIIDGFASPRMAKRFKALLARYQAAHPEVPPALAALAVA RDPAFGPRGMTANTVIAGRSDGDGEDAGLITPFRANPKAAVRTMGNAVYEVWEIQVKGRPRW THRVLTRFDRTQPAPPPPPENARLVMRLRRGDLVYWPLESGDRLFLVKKMAVDGRLALWPAR LATGKATALYAQLSCPNINLNGDQGYCVQSAEGIRKEKIRTTSCTALGRLRLSKKAT

SEQ ID NO: 367

MKYTLGLDVGIASVGWAVIDKDNNKIIDLGVRCFDKAEESKTGESLATARRIARGMRRRISR RSQRLRLVKKLFVQYEIIKDSSEFNRIFDTSRDGWKDPWELRYNALSRILKPYELVQVLTHI TKRRGFKSNRKEDLSTTKEGWITSIKNNSEMLRTKNYRTIGEMIFMETPENSNKRNKVDEY IHTIAREDLLNEIKYIFSIQRKLGSPFVTEKLEHDFLNIWEFQRPFASGDSILSKVGKCTLL KEELRAPTSCYTSEYFGLLQSINNLVLVEDNNTLTLNNDQRAKIIEYAHFKNEIKYSEIRKL LDIEPEILFKAHNLTHKNPSGNNESKKFYEMKSYHKLKSTLPTDIWGKLHSNKESLDNLFYC LTVYKNDNEIKDYLQANNLDYLIEYIAKLPTFNKFKHLSLVAMKRIIPFMEKGYKYSDACNM AELDFTGSSKLEKCNKLTVEPIIENVTNPWIRALTQARKVINAIIQKYGLPYMVNIELARE AGMTRQDRDNLKKEHENNRKAREKISDLIRQNGRVASGLDILKWRLWEDQGGRCAYSGKPIP VCDLLNDSLTQIDHIYPYSRSMDDSYMNKVLVLTDENQNKRSYTPYEVWGSTEKWEDFEARI YSMHLPQSKEKRLLNRNFITKDLDSFISRNLNDTRYISRFLKNYIESYLQFSNDSPKSCWC VNGQCTAQLRSRWGLNKNREESDLHHALDAAVIACADRKIIKEITNYYNERENHNYKVKYPL PWHSFRQDLMETLAGVFISRAPRRKITGPAHDETIRSPKHFNKGLTSVKIPLTTVTLEKLET MVKNTKGGISDKAVYNVLKNRLIEHNNKPLKAFAEKIYKPLKNGTNGAIIRSIRVETPSYTG VFRNEGKGISDNSLMVRVDVFKKKDKYYLVPIYVAHMIKKELPSKAIVPLKPESQWELIDST HEFLFSLYQNDYLVIKTKKGITEGYYRSCHRGTGSLSLMPHFANNKNVKIDIGVRTAISIEK YNVDILGNKSIVKGEPRRGMEKYNSFKSN SEQ ID NO: 368

MIRTLGIDIGIASIGWAVIEGEYTDKGLENKEIVASGVRVFTKAENPKNKESLALPRTLARS ARRRNARKKGRIQQVKHYLSKALGLDLECFVQGEKLATLFQTSKDFLSPWELRERALYRVLD KEELARVILHIAKRRGYDDITYGVEDNDSGKIKKAIAENSKRIKEEQCKTIGEMMYKLYFQK SLNVRNKKESYNRCVGRSELREELKTIFQIQQELKSPWVNEELIYKLLGNPDAQSKQEREGL IFYQRPLKGFGDKIGKCSHIKKGENSPYRACKHAPSAEEFVALTKSINFLKNLTNRHGLCFS QEDMCVYLGKILQEAQKNEKGLTYSKLKLLLDLPSDFEFLGLDYSGKNPEKAVFLSLPSTFK LNKITQDRKTQDKIANILGANKDWEAILKELESLQLSKEQIQTIKDAKLNFSKHINLSLEAL YHLLPLMREGKRYDEGVEILQERGIFSKPQPKNRQLLPPLSELAKEESYFDIPNPVLRRALS EFRKWNALLEKYGGFHYFHIELTRDVCKAKSARMQLEKINKKNKSENDAASQLLEVLGLPN TYNNRLKCKLWKQQEEYCLYSGEKITIDHLKDQRALQIDHAFPLSRSLDDSQSNKVLCLTSS NQEKSNKTPYEWLGSDEKKWDMYVGRVYSSNFSPSKKRKLTQKNFKERNEEDFLARNLVDTG YIGRVTKEYIKHSLSFLPLPDGKKEHIRIISGSMTSTMRSFWGVQEKNRDHHLHHAQDAIII ACIEPSMIQKYTTYLKDKETHRLKSHQKAQILREGDHKLSLRWPMSNFKDKIQESIQ I IPS HHVSHKVTGELHQETVRTKEFYYQAFGGEEGVKKALKFGKIREINQGIVDNGAMVRVDIFKS KDKGKFYAVPIYTYDFAIGKLPNKAIVQGKKNGIIKDWLEMDENYEFCFSLFKNDCIKIQTK EMQEAVLAIYKSTNSAKATIELEHLSKYALKNEDEEKMFTDTDKEKNKTMTRESCGIQGLKV FQKVKLSVLGEVLEHKPRNRQNIALKTTPKHV

SEQ ID NO: 369

MKYSIGLDIGIASVGWSVINKDKERIEDMGVRIFQKAENPKDGSSLASSRREKRGSRRRNRR KKHRLDRIKNILCESGLVKKNEIEKIYKNAYLKSPWELRAKSLEAKISNKEIAQILLHIAKR RGFKSFRKTDRNADDTGKLLSGIQENKKIMEEKGYLTIGDMVAKDPKFNTHVRNKAGSYLFS FSRKLLEDEVRKIQAKQKELGNTHFTDDVLEKYIEVFNSQRNFDEGPSKPSPYYSEIGQIAK MIGNCTFESSEKRTAKNTWSGERFVFLQKLNNFRIVGLSGKRPLTEEERDIVEKEVYLKKEV RYEKLRKILYLKEEERFGDLNYSKDEKQDKKTEKTKFISLIGNYTIKKLNLSEKLKSEIEED KSKLDKI IEILTFNKSDKTIESNLKKLELSREDIEILLSEEFSGTLNLSLKAIKKILPYLEK GLSYNEACEKADYDYKNNGIKFKRGELLPWDKDLIANPWLRAISQTRKVVNAI IRKYGTP HTIHVEVARDLAKSYDDRQTIIKENKKRELENEKTKKFISEEFGIKNVKGKLLLKYRLYQEQ EGRCAYSRKELSLSEVILDESMTDIDHIIPYSRSMDDSYSNKVLVLSGENRKKSNLLPKEYF DRQGRDWDTFVLNVKAMKIHPRKKSNLLKEKFTREDNKDWKSRALNDTRYISRFVANYLENA LEYRDDSPKKRVFMIPGQLTAQLRARWRLNKVRENGDLHHALDAAWAVTDQKAINNISNIS RYKELKNCKDVIPSIEYHADEETGEVYFEEVKDTRFPMPWSGFDLELQKRLESENPREEFYN LLSDKRYLGWFNYEEGFIEKLRPVFVSRMPNRGVKGQAHQETIRSSKKISNQIAVSKKPLNS IKLKDLEKMQGRDTDRKLYEALKNRLEEYDDKPEKAFAEPFYKPTNSGKRGPLVRGIKVEEK QNVGVYVNGGQASNGSMVRIDVFRKNGKFYTVPIYVHQTLLKELPNRAINGKPYKDWDLIDG SFEFLYSFYPNDLIEIEFGKSKSIKNDNKLTKTEIPEVNLSEVLGYYRGMDTSTGAATIDTQ DGKIQMRIGIKTVKNIKKYQVDVLGNVYKVKREKRQTF

SEQ ID NO: 370

MSKKVSRRYEEQAQEICQRLGSRPYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFI PSTGAAERRQKRGQRNSLRHRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAWRAN PYELRLKGLNEQLTLSELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKE KGISTFIEVLTAFNTNGLIGYRNSESVKSKGVPVPTRDI ISNEIDVLLQTQKQFYQEILSDE YCDRIVSAILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAA KRYQSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFRR LEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEWDALKTVSLIGDYGPI GKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGSTQALMGKYW HSAFKEKRDSEGFFKPNTNSDEEKYGRIANPWHQTLNELRKLMNELITILGAKPQEITVEL ARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIERFRLLEDQAFVCPYCLEH ISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIKGKRTPYAAFSNTSAWGPIMHY LDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESDNSYIAKAAKEYLRCLFNPNNVTAVG SLKGMETSILRKAWNLQGIDDLLGSRHWSKDADTSPTMRKNRDDNRHHGLDAIVALYCSRSL VQMINTMSEQGKRAVEIEAMIPIPGYASEPNLSFEAQRELFRKKILEFMDLHAFVSMKTDND ANGALLKDTVYSILGADTQGEDLVFWKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPM EMKDKIEQLQSKNEAALQKYKESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVG GYYYLISNNKRTKTFWKEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEI IRKIQAMNPSY KPAYMKQGYSLYVRLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVII ITFTEMG SGYQIYFSNLAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCG E SEQ ID NO: 371

MNQKFILGLDIGITSVGYGLIDYETKNIIDAGVRLFPEANVENNEGRRSKRGSRRLKRRRIH RLERVKKLLEDYNLLDQSQIPQSTNPYAIRVKGLSEALSKDELVIALLHIAKRRGIHKIDVI DSNDDVGNELSTKEQLNKNSKLLKDKFVCQIQLERMNEGQVRGEKNRFKTADIIKEIIQLLN VQKNFHQLDENFINKYIELVEMRREYFEGPGKGSPYGWEGDPKAWYETLMGHCTYFPDELRS VKYAYSADLFNALNDLNNLVIQRDGLSKLEYHEKYHIIENVFKQKKKPTLKQIANEINVNPE DIKGYRITKSGKPQFTEFKLYHDLKSVLFDQSILENEDVLDQIAEILTIYQDKDSIKSKLTE LDILLNEEDKENIAQLTGYTGTHRLSLKCIRLVLEEQWYSSRNQMEIFTHLNIKPKKINLTA ANKIPKAMIDEFILSPWKRTFGQAINLINKIIEKYGVPEDII IELARENNSKDKQKFINEM QKKNENTRKRINEIIGKYGNQNAKRLVEKIRLHDEQEGKCLYSLESIPLEDLLNNPNHYEVD HIIPRSVSFDNSYHNKVLVKQSENSKKSNLTPYQYFNSGKSKLSYNQFKQHILNLSKSQDRI SKKKKEYLLEERDINKFEVQKEFINRNLVDTRYATRELTNYLKAYFSANNMNVKVKTINGSF TDYLRKVWKFKKERNHGYKHHAEDALI IANADFLFKENKKLKAVNSVLEKPEIESKQLDIQV DSEDNYSEMFI IPKQVQDIKDFRNFKYSHRVDKKPNRQLINDTLYSTRKKDNSTYIVQTIKD IYAKDNTTLKKQFDKSPEKFLMYQHDPRTFEKLEVIMKQYANEKNPLAKYHEETGEYLTKYS KKNNGPIVKSLKYIGNKLGSHLDVTHQFKSSTKKLVKLSIKPYRFDVYLTDKGYKFITISYL DVLKKDNYYYIPEQKYDKLKLGKAIDKNAKFIASFYKNDLIKLDGEIYKIIGVNSDTRNMIE LDLPDIRYKEYCELNNIKGEPRIKKTIGKKVNSIEKLTTDVLGNVFTNTQYTKPQLLFKRGN

SEQ ID NO: 372

MIMKLEKWRLGLDLGTNSIGWSVFSLDKDNSVQDLIDMGVRIFSDGRDPKTKEPLAVARRTA RSQRKLIYRRKLRRKQVFKFLQEQGLFPKTKEECMTLKSLNPYELRIKALDEKLEPYELGRA LFNLAVRRGFKSNRKDGSREEVSEKKSPDEIKTQADMQTHLEKAIKENGCRTITEFLYKNQG ENGGIRFAPGRMTYYPTRKMYEEEFNLIRSKQEKYYPQVDWDDIYKAIFYQRPLKPQQRGYC IYENDKERTFKAMPCSQKLRILQDIGNLAYYEGGSKKRVELNDNQDKVLYELLNSKDKVTFD QMRKALCLADSNSFNLEENRDFLIGNPTAVKMRSKNRFGKLWDEIPLEEQDLIIETIITADE DDAVYEVIKKYDLTQEQRDFIVKNTILQSGTSMLCKEVSEKLVKRLEEIADLKYHEAVESLG YKFADQTVEKYDLLPYYGKVLPGSTMEIDLSAPETNPEKHYGKISNPTVHVALNQTRWVNA LIKEYGKPSQIAIELSRDLKNNVEKKAEIARKQNQRAKENIAINDTISALYHTAFPGKSFYP NRNDRMKYRLWSELGLGNKCIYCGKGISGAELFTKEIEIEHILPFSRTLLDAESNLTVAHSS CNAFKAERSPFEAFGTNPSGYSWQEIIQRANQLKNTSKKNKFSPNAMDSFEKDSSFIARQLS DNQYIAKAALRYLKCLVENPSDVWTTNGSMTKLLRDKWEMDSILCRKFTEKEVALLGLKPEQ IGNYKKNRFDHRHHAIDAVVIGLTDRSMVQKLATKNSHKGNRIEIPEFPILRSDLIEKVKNI WSFKPDHGAEGKLSKETLLGKIKLHGKETFVCRENIVSLSEKNLDDIVDEIKSKVKDYVAK HKGQKIEAVLSDFSKENGIKKVRCVNRVQTPIEITSGKISRYLSPEDYFAAVIWEIPGEKKT FKAQYIRRNEVEKNSKGLNWKPAVLENGKPHPAAKQVCLLHKDDYLEFSDKGKMYFCRIAG YAATNNKLDIRPVYAVSYCADWINSTNETMLTGYWKPTPTQNWVSVNVLFDKQKARLVTVSP IGRVFRK

SEQ ID NO: 373

MSSKAIDSLEQLDLFKPQEYTLGLDLGIKSIGWAILSGERIANAGVYLFETAEELNSTGNKL ISKAAERGRKRRIRRMLDRKARRGRHIRYLLEREGLPTDELEEWVHQSNRTLWDVRAEAVE RKLTKQELAAVLFHLVRHRGYFPNTKKLPPDDESDSADEEQGKINRATSRLREELKASDCKT IGQFLAQNRDRQRNREGDYSNLMARKLVFEEALQILAFQRKQGHELSKDFEKTYLDVLMGQR SGRSPKLGNCSLIPSELRAPSSAPSTEWFKFLQNLGNLQISNAYREEWSIDAPRRAQIIDAC SQRSTSSYWQIRRDFQIPDEYRFNLVNYERRDPDVDLQEYLQQQERKTLANFRNWKQLEKII GTGHPIQTLDEAARLITLIKDDEKLSDQLADLLPEASDKAITQLCELDFTTAAKISLEAMYR ILPHMNQGMGFFDACQQESLPEIGVPPAGDRVPPFDEMYNPWNRVLSQSRKLINAVIDEYG MPAKIRVELARDLGKGRELRERIKLDQLDKSKQNDQRAEDFRAEFQQAPRGDQSLRYRLWKE QNCTCPYSGRMIPVNSVLSEDTQIDHILPISQSFDNSLSNKVLCFTEENAQKSNRTPFEYLD AADFQRLEAISGNWPEAKRNKLLHKSFGKVAEEWKSRALNDTRYLTSALADHLRHHLPDSKI QTVNGRITGYLRKQWGLEKDRDKHTHHAVDAIWACTTPAIVQQVTLYHQDIRRYKKLGEKR PTPWPETFRQDVLDVEEEIFITRQPKKVSGGIQTKDTLRKHRSKPDRQRVALTKVKLADLER LVEKDASNRNLYEHLKQCLEESGDQPTKAFKAPFYMPSGPEAKQRPILSKVTLLREKPEPPK QLTELSGGRRYDSMAQGRLDIYRYKPGGKRKDEYRWLQRMIDLMRGEENVHVFQKGVPYDQ GPEIEQNYTFLFSLYFDDLVEFQRSADSEVIRGYYRTFNIANGQLKISTYLEGRQDFDFFGA NRLAHFAKVQVNLLGKVIK

SEQ ID NO: 374

MRSLRYRLALDLGSTSLGWALFRLDACNRPTAVIKAGVRIFSDGRNPKDGSSLAVTRRAARA MRRRRDRLLKRKTRMQAKLVEHGFFPADAGKRKALEQLNPYALRAKGLQEALLPGEFARALF HINQRRGFKSNRKTDKKDNDSGVLKKAIGQLRQQMAEQGSRTVGEYLWTRLQQGQGVRARYR EKPYTTEEGKKRIDKSYDLYIDRAMIEQEFDALWAAQAAFNPTLFHEAARADLKDTLLHQRP LRPVKPGRCTLLPEEERAPLALPSTQRFRIHQEVNHLRLLDENLREVALTLAQRDAWTALE TKAKLSFEQIRKLLKLSGSVQFNLEDAKRTELKGNATSAALARKELFGAAWSGFDEALQDEI VWQLVTEEGEGALIAWLQTHTGVDEARAQAIVDVSLPEGYGNLSRKALARIVPALRAAVITY DKAVQAAGFDHHSQLGFEYDASEVEDLVHPETGEIRSVFKQLPYYGKALQRHVAFGSGKPED PDEKRYGKIANPTVHIGLNQVRMWNALIRRYGRPTEWIELARDLKQSREQKVEAQRRQAD NQRRNARIRRSIAEVLGIGEERVRGSDIQKWICWEELSFDAADRRCPYSGVQISAAMLLSDE VEVEHILPFSKTLDDSLNNRTVAMRQANRIKRNRTPWDARAEFEAQGWSYEDILQRAERMPL RKRYRFAPDGYERWLGDDKDFLARALNDTRYLSRVAAEYLRLVCPGTRVIPGQLTALLRGKF GLNDVLGLDGEKNRNDHRHHAVDACVIGVTDQGLMQRFATASAQARGDGLTRLVDGMPMPWP TYRDHVERAVRHIWVSHRPDHGFEGAMMEETSYGIRKDGSIKQRRKADGSAGREISNLIRIH EATQPLRHGVSADGQPLAYKGYVGGSNYCIEITVNDKGKWEGEVISTFRAYGWRAGGMGRL RNPHEGQNGRKLIMRLVIGDSVRLEVDGAERTMRIVKISGSNGQIFMAPIHEANVDARNTDK QDAFTYTSKYAGSLQKAKTRRVTISPIGEVRDPGFKG

SEQ ID NO: 375

MARPAFRAPRREHVNGWTPDPHRISKPFFILVSWHLLSRWIDSSSGCFPGTSRDHTDKFAE WECAVQPYRLSFDLGTNSIGWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLA RQMRRRRDRYLTRRTRLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGR ALFHLNQRRGYKPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRA RLAGKGKEAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPV GRCTLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPKA GRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTALPLEE QDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVAELLPVLERE TRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLERHVAFGTGNPADPE EKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLKRSAEDRRREDKRQADNQ KRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPYSGETISMRMLLSEQVDIDHIL PFSVSLDDSAANKWCLREANRIKRNRSPWEAFGHDSERWAGILARAEALPKNKRWRFAPDA LEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVCPKVRVSPGRLTALLRRRWGIDAILAEADG PPPEVPAETLDPSPAEKNRADHRHHALDAWIGCIDRSMVQRVQLAAASAEREAAARED IR RVLEGFKEEPWDGFRAELERRARTIWSHRPEHGIGGALHKETAYGPVDPPEEGFNLWRKP IDGLSKDEINSVRDPRLRRALIDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLK KESNPIRVEHGGNPSGPRSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGA DGAAAPPRLGDGERFLMRLHKGDCLKLEHKGRVRVMQWKLEPSSNSWWEPHQVKTDRSK HVKISCDQLRARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS

SEQ ID NO: 376

MKRTSLRAYRLGVDLGANSLGWFWWLDDHGQPEGLGPGGVRIFPDGRNPQSKQSNAAGRRL ARSARRRRDRYLQRRGKLMGLLVKHGLMPADEPARKRLECLDPYGLRAKALDEVLPLHHVGR ALFHLNQRRGLFANRAIEQGDKDASAIKAAAGRLQTSMQACGARTLGEFLNRRHQLRATVRA RSPVGGDVQARYEFYPTRAMVDAEFEAIWAAQAPHHPTMTAEAHDTIREAIFSQRAMKRPSI GKCSLDPATSQDDVDGFRCAWSHPLAQRFRIWQDVRNLAWETGPTSSRLGKEDQDKVARAL LQTDQLSFDEIRGLLGLPSDARFNLESDRRDHLKGDATGAILSARRHFGPAWHDRSLDRQID IVALLESALDEAAIIASLGTTHSLDEAAAQRALSALLPDGYCRLGLRAIKRVLPLMEAGRTY AEAASAAGYDHALLPGGKLSPTGYLPYYGQWLQNDWGSDDERDTNERRWGRLPNPTVHIGI GQLRRWNELIRWHGPPAEITVELTRDLKLSPRRLAELEREQAENQRKNDKRTSLLRKLGLP ASTHNLLKLRLWDEQGDVASECPYTGEAIGLERLVSDDVDIDHLIPFSISWDDSAANKVVCM RYANREKGNRTPFEAFGHRQGRPYDWADIAERAARLPRGKRWRFGPGARAQFEELGDFQARL LNETSWLARVAKQYLAAVTHPHRIHVLPGRLTALLRATWELNDLLPGSDDRAAKSRKDHRHH AIDALVAALTDQALLRRMANAHDDTRRKIEVLLPWPTFRIDLETRLKAMLVSHKPDHGLQAR LHEDTAYGTVEHPETEDGANLVYRKTFVDISEKEIDRIRDRRLRDLVRAHVAGERQQGKTLK AAVLSFAQRRDIAGHPNGIRHVRLTKSIKPDYLVPIRDKAGRIYKSYNAGENAFVDILQAES GRWIARATTVFQANQANESHDAPAAQPIMRVFKGDMLRIDHAGAEKFVKIVRLSPSNNLLYL VEHHQAGVFQTRHDDPEDSFRWLFASFDKLREWNAELVRIDTLGQPWRRKRGLETGSEDATR IGWTRPKKWP

SEQ ID NO: 377

MERIFGFDIGTTSIGFSVIDYSSTQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQ LRRRRIRRKALNETLHEAGFLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLA QHRHFKGRELEESDTPDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHA HRNWAEEFERLWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCP KGSWLSQQRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQR GEPGAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYGE TPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPALNLFLA ELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQLRNPTVVRT QNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQRKKATEDLIKNGI ANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIWPRSRSFDNSPRNKTLCR KDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTGMSPGKVKRFLAKTMPEDFAAR QLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQVTAQLRKLWTLNNILADDGEKTRAD HRHHAIDALTVACTHPGMTNKLSRYWQLRDDPRAEKPALTPPWDTIRADAEKAVSEIWSHR VRKKVSGPLHKETTYGDTGTDIKTKSGTYRQFVTRKKIESLSKGELDEIRDPRIKEIVAAHV AGRGGDPKKAFPPYPCVSPGGPEIRKVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDG KADFEIVSLFDASRRLAQRNPIVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWAS GQVVLERDTDADHSTTTRPMPNPILKDDAKKVSIDPIGRVRPSND

SEQ ID NO: 378

MNKRILGLDTGTNSLGWAVVDWDEHAQSYELIKYGDVIFQEGVKIEKGIESSKAAERSGYKA IRKQYFRRRLRKIQVLKVLVKYHLCPYLSDDDLRQWHLQKQYPKSDELMLWQRTSDEEGKNP YYDRHRCLHEKLDLTVEADRYTLGRALYHLTQRRGFLSNRLDTSADNKEDGWKSGISQLST EMEEAGCEYLGDYFYKLYDAQGNKVRIRQRYTDRNKHYQHEFDAICEKQELSSELIEDLQRA IFFQLPLKSQRHGVGRCTFERGKPRCADSHPDYEEFRMLCFVNNIQVKGPHDLELRPLTYEE REKIEPLFFRKSKPNFDFEDIAKALAGKKNYAWIHDKEERAYKFNYRMTQGVPGCPTIAQLK SIFGDDWKTGIAETYTLIQKKNGSKSLQEMVDDVWNVLYSFSSVEKLKEFAHHKLQLDEESA EKFAKIKLSHSFAALSLKAIRKFLPFLRKGMYYTHASFFANIPTIVGKEIWNKEQNRKYIME NVGELVFNYQPKHREVQGTIEMLIKDFLANNFELPAGATDKLYHPSMIETYPNAQRNEFGIL QLGSPRTNAIRNPMAMRSLHILRRWNQLLKESIIDENTEVHVEYARELNDANKRRAIADRQ KEQDKQHKKYGDEIRKLYKEETGKDIEPTQTDVLKFQLWEEQNHHCLYTGEQIGITDFIGSN PKFDIEHTIPQSVGGDSTQMNLTLCDNRFNREVKKAKLPTELANHEEILTRIEPWKNKYEQL VKERDKQRTFAGMDKAVKDIRIQKRHKLQMEIDYWRGKYERFTMTEVPEGFSRRQGTGIGLI SRYAGLYLKSLFHQADSRNKSNVYWKGVATAEFRKMWGLQSEYEKKCRDNHSHHCMDAITI ACIGKREYDLMAEYYRMEETFKQGRGSKPKFSKPWATFTEDVLNIYKNLLVVHDTPNNMPKH TKKYVQTSIGKVLAQGDTARGSLHLDTYYGAIERDGEIRYWRRPLSSFTKPEELENIVDET VKRTIKEAIADKNFKQAIAEPIYMNEEKGILIKKVRCFAKSVKQPINIRQHRDLSKKEYKQQ YHVMNENNYLLAIYEGLVKNKWREFEIVSYIEAAKYYKRSQDRNIFSSIVPTHSTKYGLPL KTKLLMGQLVLMFEENPDEIQVDNTKDLVKRLYKVVGIEKDGRIKFKYHQEARKEGLPIFST PYKNNDDYAPIFRQSINNINILVDGIDFTIDILGKVTLKE

SEQ ID NO: 379

MNYKMGLDIGIASVGWAVINLDLKRIEDLGVRIFDKAEHPQNGESLALPRRIARSARRRLRR RKHRLERIRRLLVSENVLTKEEMNLLFKQKKQIDVWQLRVDALERKLNNDELARVLLHLAKR RGFKSNRKSERNSKESSEFLKNIEENQSILAQYRSVGEMIVKDSKFAYHKRNKLDSYSNMIA RDDLEREIKLIFEKQREFNNPVCTERLEEKYLNIWSSQRPFASKEDIEKKVGFCTFEPKEKR APKATYTFQSFIVWEHINKLRLVSPDETRALTEIERNLLYKQAFSKNKMTYYDIRKLLNLSD DIHFKGLLYDPKSSLKQIENIRFLELDSYHKIRKCIENVYGKDGIRMFNETDIDTFGYALTI FKDDEDIVAYLQNEYITKNGKRVSNLANKVYDKSLIDELLNLSFSKFAHLSMKAIRNILPYM EQGEIYSKACELAGYNFTGPKKKEKALLLPVIPNIANPVVMRALTQSRKWNAIIKKYGSPV SIHIELARDLSHSFDERKKIQKDQTENRKKNETAIKQLIEYELTKNPTGLDIVKFKLWSEQQ GRCMYSLKPIELERLLEPGYVEVDHILPYSRSLDDSYANKVLVLTKENREKGNHTPVEYLGL GSERWKKFEKFVLANKQFSKKKKQNLLRLRYEETEEKEFKERNLNDTRYISKFFANFIKEHL KFADGDGGQKVYTINGKITAHLRSRWDFNKNREESDLHHAVDAVIVACATQGMIKKITEFYK AREQNKESAKKKEPIFPQPWPHFADELKARLSKFPQESIEAFALGNYDRKKLESLRPVFVSR MPKRSVTGAAHQETLRRCVGIDEQSGKIQTAVKTKLSDIKLDKDGHFPMYQKESDPRTYEAI RQRLLEHNNDPKKAFQEPLYKPKKNGEPGPVIRTVKIIDTKNKWHLDGSKTVAYNSNIVRT DVFEKDGKYYCVPVYTMDIMKGTLPNKAIEANKPYSEWKEMTEEYTFQFSLFPNDLVRIVLP REKTIKTSTNEEIIIKDIFAYYKTIDSATGGLELISHDRNFSLRGVGSKTLKRFEKYQVDVL GNIHKVKGEKRVGLAAPTNQKKGKTVDSLQSVSD

SEQ ID NO: 380

MRRLGLDLGTNSIGWCLLDLGDDGEPVSIFRTGARIFSDGRDPKSLGSLKATRREARLTRRR RDRFIQRQKNLINALVKYGLMPADEIQRQALAYKDPYPIRKKALDEAIDPYEMGRAIFHINQ RRGFKSNRKSADNEAGWKQSIADLEMKLGEAGARTIGEFLADRQATNDTVRARRLSGTNAL YEFYPDRYMLEQEFDTLWAKQAAFNPSLYIEAARERLKEIVFFQRKLKPQEVGRCIFLSDED RISKALPSFQRFRIYQELSNLAWIDHDGVAHRITASLALRDHLFDELEHKKKLTFKAMRAIL RKQGWDYPVGFNLESDNRDHLIGNLTSCIMRDAKKMIGSAWDRLDEEEQDSFILMLQDDQK GDDEVRSILTQQYGLSDDVAEDCLDVRLPDGHGSLSKKAIDRILPVLRDQGLIYYDAVKEAG LGEANLYDPYAALSDKLDYYGKALAGHVMGASGKFEDSDEKRYGTISNPTVHIALNQVRAW NELIRLHGKPDEWIEIGRDLPMGADGKRELERFQKEGRAKNERARDELKKLGHIDSRESRQ KFQLWEQLAKEPVDRCCPFTGKMMSISDLFSDKVEIEHLLPFSLTLDDSMANKTVCFRQANR DKGNRAPFDAFGNSPAGYDWQEILGRSQNLPYAKRWRFLPDAMKRFEADGGFLERQLNDTRY ISRYTTEYISTIIPKNKIWWTGRLTSLLRGFWGLNSILRGHNTDDGTPAKKSRDDHRHHAI DAIWGMTSRGLLQKVSKAARRSEDLDLTRLFEGRIDPWDGFRDEVKKHIDAIIVSHRPRKK SQGALHNDTAYGIVEHAENGASTWHRVPITSLGKQSDIEKVRDPLIKSALLNETAGLSGKS FENAVQKWCADNSIKSLRIVETVSIIPITDKEGVAYKGYKGDGNAYMDIYQDPTSSKWKGEI VSRFDANQKGFIPSWQSQFPTARLIMRLRINDLLKLQDGEIEEIYRVQRLSGSKILMAPHTE ANVDARDRDKNDTFKLTSKSPGKLQSASARKVHISPTGLIREG

SEQ ID NO: 381

MKNILGLDLGLSSIGWSVIRENSEEQELVAMGSRVVSLTAAELSSFTQGNGVSINSQRTQKR TQRKGYDRYQLRRTLLRNKLDTLGMLPDDSLSYLPKLQLWGLRAKAVTQRIELNELGRVLLH LNQKRGYKSIKSDFSGDKKITDYVKTVKTRYDELKEMRLTIGELFFRRLTENAFFRCKEQVY PRQAYVEEFDCIMNCQRKFYPDILTDETIRCIRDEIIYYQRPLKSCKYLVSRCEFEKRFYLN AAGKKTEAGPKVSPRTSPLFQVCRLWESINNIWKDRRNEIVFISAEQRAALFDFLNTHEKL KGSDLLKLLGLSKTYGYRLGEQFKTGIQGNKTRVEIERALGNYPDKKRLLQFNLQEESSSMV NTETGEI IPMISLSFEQEPLYRLWHVLYSIDDREQLQSVLRQKFGIDDDEVLERLSAIDLVK AGFGNKSSKAIRRILPFLQLGMNYAEACEAAGYNHSNNYTKAENEARALLDRLPAIKKNELR QPVVEKILNQMVNWNALMEKYGRFDEIRVELARELKQSKEERSNTYKSINKNQRENEQIAK RIVEYGVPTRSRIQKYKMWEESKHCCIYCGQPVDVGDFLRGFDVEVEHIIPKSLYFDDSFAN KVCSCRSCNKEKNNRTAYDYMKSKGEKALSDYVERVNTMYTNNQISKTKWQNLLTPVDKISI DFIDRQLRESQYIARKAKEILTSICYNVTATSGSVTSFLRHVWGWDTVLHDLNFDRYKKVGL TEVIEVNHRGSVIRREQIKDWSKRFDHRHHAIDALTIACTKQAYIQRLNNLRAEEGPDFNKM SLERYIQSQPHFSVAQVREAVDRILVSFRAGKRAVTPGKRYIRKNRKRISVQSVLIPRGALS EESVYGVIHVWEKDEQGHVIQKQRAVMKYPITSINREMLDKEKWDKRIHRILSGRLAQYND NPKEAFAKPVYIDKECRIPIRTVRCFAKPAINTLVPLKKDDKGNPVAWVNPGNNHHVAIYRD EDGKYKERTVTFWEAVDRCRVGIPAIVTQPDTIWDNILQRNDISENVLESLPDVKWQFVLSL QQNEMFILGMNEEDYRYAMDQQDYALLNKYLYRVQKLSKSDYSFRYHTETSVEDKYDGKPNL KLSMQMGKLKRVSIKSLLGLNPHKVHISVLGEIKEIS SEQ ID NO: 382

MAEKQHRWGLDIGTNSIGWAVIALIEGRPAGLVATGSRIFSDGRNPKDGSSLAVERRGPRQM RRRRDRYLRRRDRFMQALINVGLMPGDAAARKALVTENPYVLRQRGLDQALTLPEFGRALFH LNQRRGFQSNRKTDRATAKESGKVKNAIAAFRAGMGNARTVGEALARRLEDGRPVRARMVGQ GKDEHYELYIAREWIAQEFDALWASQQRFHAEVLADAARDRLRAILLFQRKLLPVPVGKCFL EPNQPRVAAALPSAQRFRLMQELNHLRVMTLADKRERPLSFQERNDLLAQLVARPKCGFDML RKIVFGANKEAYRFTIESERRKELKGCDTAAKLAKVNALGTRWQALSLDEQDRLVCLLLDGE NDAVLADALREHYGLTDAQIDTLLGLSFEDGHMRLGRSALLRVLDALESGRDEQGLPLSYDK AWAAGYPAHTADLENGERDALPYYGELLWRYTQDAPTAKNDAERKFGKIANPTVHIGLNQL RKLVNALIQRYGKPAQIWELARNLKAGLEEKERIKKQQTANLERNERIRQKLQDAGVPDNR ENRLRMRLFEELGQGNGLGTPCIYSGRQISLQRLFSNDVQVDHILPFSKTLDDSFANKVLAQ HDANRYKGNRGPFEAFGANRDGYAWDDIRARAAVLPRNKRNRFAETAMQDWLHNETDFLARQ LTDTAYLSRVARQYLTAICSKDDVYVSPGRLTAMLRAKWGLNRVLDGVMEEQGRPAVKNRDD HRHHAIDAWIGATDRAMLQQVATLAARAREQDAERLIGDMPTPWPNFLEDVRAAVARCWS HKPDHGPEGGLHNDTAYGIVAGPFEDGRYRVRHRVSLFDLKPGDLSNVRCDAPLQAELEPIF EQDDARAREVALTALAERYRQRKVWLEELMSVLPIRPRGEDGKTLPDSAPYKAYKGDSNYCY ELFINERGRWDGELISTFRANQAAYRRFRNDPARFRRYTAGGRPLLMRLCINDYIAVGTAAE RTIFRWKMSENKITLAEHFEGGTLKQRDADKDDPFKYLTKSPGALRDLGARRIFVDLIGRV LDPGIKGD

SEQ ID NO: 383

MIERILGVDLGISSLGWAIVEYDKDDEAANRIIDCGVRLFTAAETPKKKESPNKARREARGI RRVLNRRRVRMNMIKKLFLRAGLIQDVDLDGEGGMFYSKANRADVWELRHDGLYRLLKGDEL ARVLIHIAKHRGYKFIGDDEADEESGKVKKAGWLRQNFEAAGCRTVGEWLWRERGANGKKR NKHGDYEISIHRDLLVEEVEAIFVAQQEMRSTIATDALKAAYREIAFFVRPMQRIEKMVGHC TYFPEERRAPKSAPTAEKFIAISKFFSTVIIDNEGWEQKIIERKTLEELLDFAVSREKVEFR HLRKFLDLSDNEIFKGLHYKGKPKTAKKREATLFDPNEPTELEFDKVEAEKKAWISLRGAAK LREALGNEFYGRFVALGKHADEATKILTYYKDEGQKRRELTKLPLEAEMVERLVKIGFSDFL KLSLKAIRDILPAMESGARYDEAVLMLGVPHKEKSAILPPLNKTDIDILNPTVIRAFAQFRK VANALVRKYGAFDRVHFELAREINTKGEIEDIKESQRKNEKERKEAADWIAETSFQVPLTRK NILKKRLYIQQDGRCAYTGDVIELERLFDEGYCEIDHILPRSRSADDSFANKVLCLARANQQ KTDRTPYEWFGHDAARWNAFETRTSAPSNRVRTGKGKIDRLLKKNFDENSEMAFKDRNLNDT RYMARAIKTYCEQYWVFKNSHTKAPVQVRSGKLTSVLRYQWGLESKDRESHTHHAVDAI IIA FSTQGMVQKLSEYYRFKETHREKERPKLAVPLANFRDAVEEATRIENTETVKEGVEVKRLLI SRPPRARVTGQAHEQTAKPYPRIKQVKNKKKWRLAPIDEEKFESFKADRVASANQKNFYETS TIPRVDVYHKKGKFHLVPIYLHEMVLNELPNLSLGTNPEAMDENFFKFSIFKDDLISIQTQG TPKKPAKIIMGYFKNMHGANMVLSSINNSPCEGFTCTPVSMDKKHKDKCKLCPEENRIAGRC LQGFLDYWSQEGLRPPRKEFECDQGVKFALDVKKYQIDPLGYYYEVKQEKRLGTIPQMRSAK KLVKK

SEQ ID NO: 384

MNNSIKSKPEVTIGLDLGVGSVGWAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIR RRKYKLKRFVNLIWKYNSYFGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWI LHDYLKNRGHFYEDNRDFNVYPTKELAKYFDKYGYYKGI IDSKEDNDNKLEEELTKYKFSNK HWLEEVKKVLSNQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQK YNNIWDKTIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLN KLLDLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLNT NGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSNRVDSA NLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAITRMTNLDND EDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFINDAILSPGVKRILRE ATKVFNAILKQFSEEYDVTKWIELARELSEEKELENTKNYKKLIKKNGDKISEGLKALGIS EDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFTKTEKFEIDHI IPYSISFDDSS SNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEAYCRKFKDGDSSLLDSTQRSKKFAKM MKTDTSSKYDIGFLARNLNDTRYATIVFRDALEDYANNHLVEDKPMFKWCINGSVTSFLRK NFDDSSYAKKDRDKNIHHAVDASIISIFSNETKTLFNQLTQFADYKLFKNTDGSWKKIDPKT GWTEVTDENWKQIRVRNQVSEIAKVIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSA KKVGYEDQIKRKNLKTLDIHESAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDIL MYRANPWVINLAEQIFNEYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAII YDDKKNIVHRIKRLKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTA KKQYIRVPLNTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIH KKDKKLMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDA KDNFDILGLSKNRIDEILNSKLGLDKIVK

SEQ ID NO: 385

MGGSEVGTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERSGASRLA LRGMARRARRLRRFRRARLRDLDMLLSELGWTPLPDKNVSPVDAWLARKRLAEEYWDETER RRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQPSDSWERTRESLEARYSVSLEPGTVGQWAG YLLQRAPGIRLNPTQQSAGRRAELSNATAFETRLRQEDVLWELRCIADVQGLPEDWSNVID AVFCQKRPSVPAERIGRDPLDPSQLRASRACLEFQEYRIVAAVANLRIRDGSGSRPLSLEER NAVIEALLAQTERSLTWSDIALEILKLPNESDLTSVPEEDGPSSLAYSQFAPFDETSARIAE FIAKNRRKIPTFAQWWQEQDRTSRSDLVAALADNSIAGEEEQELLVHLPDAELEALEGLALP SGRVAYSRLTLSGLTRVMRDDGVDVHNARKTCFGVDDNWRPPLPALHEATGHPWDRNLAIL RKFLSSATMRWGPPQSIWELARGASESRERQAEEEAARRAHRKANDRIRAELRASGLSDPS PADLVRARLLELYDCHCMYCGAPISWENSELDHIVPRTDGGSNRHENLAITCGACNKEKGRR PFASWAETSNRVQLRDVIDRVQKLKYSGNMYWTRDEFSRYKKSWARLKRRTSDPEVIQSIE STGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARRWLDISIERLFSRVAIFAQSTSTKR LDRRHHAVDAVVLTTLTPGVAKTLADARSRRVSAEFWRRPSDVNRHSTEEPQSPAYRQWKES CSGLGDLLISTAARDSIAVAAPLRLRPTGALHEETLRAFSEHTVGAAWKGAELRRIVEPEVY AAFLALTDPGGRFLKVSPSEDVLPADENRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGAAY IASFHHARVFRWGSSHSPSFALLRVSLADLAVAGLLRDGVDVFTAELPPWTPAWRYASIALV KAVESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSMFLKYFPERHWVVTGFEDDKRINLKPA FLSAEQAEVLRTERSDRPDTLTEAGEILAQFFPRCWRATVAKVLCHPGLTVIRRTALGQPRW RRGHLPYSWRPWSADPWSGGTP

SEQ ID NO: 386

MHNKKNITIGFDLGIASIGWAIIDSTTSKILDWGTRTFEERKTANERRAFRSTRRNIRRKAY RNQRFINLILKYKDLFELKNISDIQRANKKDTENYEKIISFFTEIYKKCAAKHSNILEVKVK ALDSKIEKLDLIWILHDYLENRGFFYDLEEENVADKYEGIEHPSILLYDFFKKNGFFKSNSS IPKDLGGYSFSNLQWVNEIKKLFEVQEINPEFSEKFLNLFTSVRDYAKGPGSEHSASEYGIF QKDEKGKVFKKYDNIWDKTIGKCSFFVEENRSPVNYPSYEIFNLLNQLINLSTDLKTTNKKI WQLSSNDRNELLDELLKVKEKAKIISISLKKNEIKKIILKDFGFEKSDIDDQDTIEGRKIIK EEPTTKLEVTKHLLATIYSHSSDSNWININNILEFLPYLDAICIILDREKSRGQDEVLKKLT EKNIFEVLKIDREKQLDFVKSIFSNTKFNFKKIGNFSLKAIREFLPKMFEQNKNSEYLKWKD EEIRRKWEEQKSKLGKTDKKTKYLNPRIFQDEIISPGTKNTFEQAVLVLNQIIKKYSKENII DAI IIESPREKNDKKTIEEIKKRNKKGKGKTLEKLFQILNLENKGYKLSDLETKPAKLLDRL RFYHQQDGIDLYTLDKINIDQLINGSQKYEIEHIIPYSMSYDNSQANKILTEKAENLKKGKL IASEYIKRNGDEFYNKYYEKAKELFINKYKKNKKLDSYVDLDEDSAKNRFRFLTLQDYDEFQ VEFLARNLNDTRYSTKLFYHALVEHFENNEFFTYIDENSSKHKVKISTIKGHVTKYFRAKPV QKNNGPNENLNNNKPEKIEKNRENNEHHAVDAAIVAIIGNKNPQIANLLTLADNKTDKKFLL HDENYKENIETGELVKIPKFEVDKLAKVEDLKKIIQEKYEEAKKHTAIKFSRKTRTILNGGL SDETLYGFKYDEKEDKYFKIIKKKLVTSKNEELKKYFENPFGKKADGKSEYTVLMAQSHLSE FNKLKEIFEKYNGFSNKTGNAFVEYMNDLALKEPTLKAEIESAKSVEKLLYYNFKPSDQFTY HDNINNKSFKRFYKNIRIIEYKSIPIKFKILSKHDGGKSFKDTLFSLYSLVYKVYENGKESY KSIPVTSQMRNFGIDEFDFLDENLYNKEKLDIYKSDFAKPIPVNCKPVFVLKKGSILKKKSL DIDDFKETKETEEGNYYFISTISKRFNRDTAYGLKPLKLSWKPVAEPSTNPIFKEYIPIHL DELGNEYPVKIKEHTDDEKLMCTIK Nucleic Acids Encoding Cas9 Molecules

Nucleic acids encoding the Cas9 molecules or Cas9 polypeptides, e.g., an eaCas9 molecule or eaCas9 poypeptides are provided herein.

Exemplary nucleic acids encoding Cas9 molecules or Cas9 polypeptides are described in Cong etal, ScffiNCE 2013, 399(6121):819-823; Wang et al, CELL 2013, 153(4):910-918; Mali et al, ScffiNCE 2013, 399(6121):823-826; Jinek et al, SCIENCE 2012, 337(6096):816- 821. Another exemplary nucleic acid encoding a Cas9 molecule or Cas9 polypeptides is shown in Fig. 8. In an embodiment, a nucleic acid encoding a Cas9 molecule or Cas9 polypeptides can be a synthetic nucleic acid sequence. For example, the synthetic nucleic acid molecule can be chemically modified, e.g., as described in Section VIII. In an embodiment, the Cas9 mRNA has one or more (e.g., all of the following properties: it is capped, polyadenylated, substituted with 5-methylcytidine and/or pseudouridine.

In addition, or alternatively, the synthetic nucleic acid sequence can be codon optimized, e.g., at least one non-common codon or less-common codon has been replaced by a common codon. For example, the synthetic nucleic acid can direct the synthesis of an optimized messenger mRNA, e.g., optimized for expression in a mammalian expression system, e.g., described herein.

In addition, or alternatively, a nucleic acid encoding a Cas9 molecule or Cas9 polypeptides may comprise a nuclear localization sequence (NLS). Nuclear localization sequences are known in the art.

Provided below is an exemplary codon optimized nucleic acid sequence encoding a Cas9 molecule of S. pyogenes.

ATGGATAAAA AGTACAGCAT CGGGCTGGAC ATCGGTACAA ACTCAGTGGG GTGGGCCGTG ATTACGGACG AGTACAAGGT ACCCTCCAAA AAATTTAAAG TGCTGGGTAA CACGGACAGA CACTCTATAA AGAAAAATCT TATTGGAGCC TTGCTGTTCG ACTCAGGCGA GACAGCCGAA GCCACAAGGT TGAAGCGGAC CGCCAGGAGG CGGTATACCA GGAGAAAGAA CCGCATATGC TACCTGCAAG AAATCTTCAG TAACGAGATG GCAAAGGTTG ACGATAGCTT TTTCCATCGC CTGGAAGAAT CCTTTCTTGT TGAGGAAGAC AAGAAGCACG AACGGCACCC CATCTTTGGC AATATTGTCG ACGAAGTGGC ATATCACGAA AAGTACCCGA CTATCTACCA CCTCAGGAAG AAGCTGGTGG ACTCTACCGA TAAGGCGGAC CTCAGACTTA TTTATTTGGC ACTCGCCCAC AT GAT T AAA T TTAGAGGACA TTTCTTGATC GAGGGCGACC TGAACCCGGA CAACAGTGAC GTCGATAAGC TGTTCATCCA ACTTGTGCAG ACCTACAATC AACTGTTCGA AGAAAACCCT ATAAATGCTT CAGGAGTCGA CGCTAAAGCA ATCCTGTCCG CGCGCCTCTC AAAATCTAGA AGACTTGAGA ATCTGATTGC TCAGTTGCCC GGGGAAAAGA AAAATGGATT GTTTGGCAAC CTGATCGCCC TCAGTCTCGG ACTGACCCCA AATTTCAAAA GTAACTTCGA CCTGGCCGAA GACGCTAAGC TCCAGCTGTC CAAGGACACA TACGATGACG ACCTCGACAA TCTGCTGGCC CAGATTGGGG ATCAGTACGC CGATCTCTTT TTGGCAGCAA AGAACCTGTC CGACGCCATC CTGTTGAGCG ATATCTTGAG AGTGAACACC GAAATTACTA AAGCACCCCT TAGCGCATCT ATGATCAAGC GGTACGACGA GCATCATCAG GATCTGACCC TGCTGAAGGC TCTTGTGAGG CAACAGCTCC CCGAAAAATA CAAGGAAATC TTCTTTGACC AG AG C AAAAA CGGCTACGCT GGCTATATAG ATGGTGGGGC CAGTCAGGAG GAATTCTATA AATTCATCAA GCCCATTCTC GAGAAAATGG ACGGCACAGA GGAGTTGCTG GTCAAACTTA ACAGGGAGGA CCTGCTGCGG AAGCAGCGGA CCTTTGACAA CGGGTCTATC CCCCACCAGA TTCATCTGGG CGAACTGCAC GCAATCCTGA GGAGGCAGGA GGATTTTTAT CCTTTTCTTA AAGATAACCG CGAGAAAATA GAAAAGATTC TTACATTCAG GATCCCGTAC TACGTGGGAC CTCTCGCCCG GGGCAATTCA CGGTTTGCCT GGATGACAAG GAAGTCAGAG GAGACTATTA CACCTTGGAA CTTCGAAGAA GTGGTGGACA AGGGTGCATC TGCCCAGTCT TTCATCGAGC GGATGACAAA TTTTGACAAG AACCTCCCTA ATGAGAAGGT GCTGCCCAAA CATTCTCTGC TCTACGAGTA CTTTACCGTC TACAATGAAC TGACTAAAGT CAAGTACGTC ACCGAGGGAA TGAGGAAGCC GGCATTCCTT AGTGGAGAAC AGAAGAAGGC GATTGTAGAC CTGTTGTTCA AGACCAACAG GAAGGTGACT GTGAAGCAAC TTAAAGAAGA CTACTTTAAG AAGATCGAAT GTTTTGACAG TGTGGAAATT TCAGGGGTTG AAGACCGCTT CAATGCGTCA TTGGGGACTT AC CAT GAT CT TCTCAAGATC ATAAAGGACA AAGACTTCCT GGACAACGAA GAAAATGAGG ATATTCTCGA AGACATCGTC CTCACCCTGA CCCTGTTCGA AGACAGGGAA ATGATAGAAG AGCGCTTGAA AACCTATGCC CACCTCTTCG ACGATAAAGT TAT GAAG C AG CTGAAGCGCA GGAGATACAC AGGATGGGGA AGATTGTCAA GGAAGCTGAT CAATGGAATT AGGGATAAAC AGAGTGGCAA GACCATACTG GATTTCCTCA AATCTGATGG CTTCGCCAAT AGGAACTTCA TGCAACTGAT TCACGATGAC TCTCTTACCT TCAAGGAGGA CATTCAAAAG GCTCAGGTGA GCGGGCAGGG AGACTCCCTT CATGAACACA TCGCGAATTT GGCAGGTTCC CCCGCTATTA AAAAGGGCAT CCTTCAAACT GTCAAGGTGG TGGATGAATT GGTCAAGGTA ATGGGCAGAC ATAAGCCAGA AAATATTGTG ATCGAGATGG CCCGCGAAAA CCAGACCACA CAGAAGGGCC AGAAAAATAG TAGAGAGCGG ATGAAGAGGA TCGAGGAGGG CATCAAAGAG CTGGGATCTC AGATTCTCAA AGAACACCCC GTAGAAAACA CACAGCTGCA GAACGAAAAA TTGTACTTGT ACTATCTGCA GAACGGCAGA GACATGTACG TCGACCAAGA AC TT GAT ATT AATAGACTGT CCGACTATGA CGTAGACCAT ATCGTGCCCC AGTCCTTCCT GAAGGACGAC TCCATTGATA ACAAAGTCTT GACAAGAAGC GACAAGAACA GGGGTAAAAG TGATAATGTG CCTAGCGAGG AGGTGGTGAA AAAAAT GAAG AACTACTGGC GACAGCTGCT TAATGCAAAG CTCATTACAC AACGGAAGTT CGATAATCTG ACGAAAGCAG AGAGAGGTGG CTTGTCTGAG TTGGACAAGG CAGGGTTTAT TAAGCGGCAG CTGGTGGAAA CTAGGCAGAT CACAAAGCAC GTGGCGCAGA TTTTGGACAG CCGGATGAAC ACAAAATACG ACGAAAATGA TAAACTGATA CGAGAGGTCA AAGTTATCAC GCTGAAAAGC AAGCTGGTGT CCGATTTTCG GAAAGACTTC CAGTTCTACA AAGTTCGCGA GATTAATAAC TACCATCATG CTCACGATGC GTACCTGAAC GCTGTTGTCG GGACCGCCTT GATAAAGAAG TACCCAAAGC TGGAATCCGA GTTCGTATAC GGGGATTACA AAGTGTACGA TGTGAGGAAA ATGATAGCCA AGTCCGAGCA GGAGATTGGA AAGGCCACAG CTAAGTACTT CTTTTATTCT AACATCATGA ATTTTTTTAA GACGGAAATT ACCCTGGCCA ACGGAGAGAT CAGAAAGCGG CCCCTTATAG AGACAAATGG TGAAACAGGT GAAATCGTCT GGGATAAGGG CAGGGATTTC GCTACTGTGA GGAAGGTGCT GAGTATGCCA CAGGTAAATA TCGTGAAAAA AACCGAAGTA CAGACCGGAG GATTTTCCAA GGAAAGCATT TTGCCTAAAA GAAACTCAGA CAAGCTCATC GCCCGCAAGA AAGATTGGGA CCCTAAGAAA TACGGGGGAT TTGACTCACC CACCGTAGCC TATTCTGTGC TGGTGGTAGC TAAGGTGGAA AAAGGAAAGT CTAAGAAGCT GAAGTCCGTG AAGGAACTCT TGGGAATCAC TATCATGGAA AG AT CATC CT TTGAAAAGAA CCCTATCGAT TTCCTGGAGG CTAAGGGTTA CAAGGAGGTC AAGAAAGACC TCATCATTAA ACTGCCAAAA TACTCTCTCT TCGAGCTGGA AAATGGCAGG AAGAGAATGT TGGCCAGCGC CGGAGAGCTG CAAAAGGGAA ACGAGCTTGC TCTGCCCTCC AAATATGTTA ATTTTCTCTA TCTCGCTTCC CACTATGAAA AGCTGAAAGG GTCTCCCGAA GATAACGAGC AGAAGCAGCT GTTCGTCGAA CAGCACAAGC ACTATCTGGA TGAAATAATC GAACAAATAA GCGAGTTCAG CAAAAGGGTT ATCCTGGCGG ATGCTAATTT GGACAAAGTA CTGTCTGCTT ATAACAAGCA CCGGGATAAG CCTATTAGGG AACAAGCCGA GAATATAATT CACCTCTTTA CACTCACGAA TCTCGGAGCC CCCGCCGCCT TCAAATACTT TGATACGACT ATCGACCGGA

AACGGTATAC CAGTACCAAA GAGGTCCTCG ATGCCACCCT CATCCACCAG TCAATTACTG GCCTGTACGA AACACGGATC GACCTCTCTC AACTGGGCGG CGACTAG

(SEQ ID NO: 22)

Provided below is the corresponding amino acid sequence of a S. pyogenes Cas9 molecule.

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEAT RLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFI QLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGL TPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNT EITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEF YKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLK DNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT NFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIV LTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKW DELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQL QNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSD NVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAV VGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLAN GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNS DKLIARKKDWDPKKYGGFDSPTVAYSVLWAKVEKGKSKKLKSVKELLGITIMERSSFEKNP IDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI REQAENI IHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ LGGD*

(SEQ ID NO: 23)

Provided below is an exemplary codon optimized nucleic acid sequence encoding a Cas9 molecule of N. meningitidis.

ATGGCCGCCTTCAAGCCCAACCCCATCAACTACATCCTGGGCCTGGACATCGGCATCGCCAG CGTGGGCTGGGCCATGGTGGAGATCGACGAGGACGAGAACCCCATCTGCCTGATCGACCTGG GTGTGCGCGTGTTCGAGCGCGCTGAGGTGCCCAAGACTGGTGACAGTCTGGCTATGGCTCGC CGGCTTGCTCGCTCTGTTCGGCGCCTTACTCGCCGGCGCGCTCACCGCCTTCTGCGCGCTCG CCGCCTGCTGAAGCGCGAGGGTGTGCTGCAGGCTGCCGACTTCGACGAGAACGGCCTGATCA AGAGCCTGCCCAACACTCCTTGGCAGCTGCGCGCTGCCGCTCTGGACCGCAAGCTGACTCCT CTGGAGTGGAGCGCCGTGCTGCTGCACCTGATCAAGCACCGCGGCTACCTGAGCCAGCGCAA GAACGAGGGCGAGACCGCCGACAAGGAGCTGGGTGCTCTGCTGAAGGGCGTGGCCGACAACG CCCACGCCCTGCAGACTGGTGACTTCCGCACTCCTGCTGAGCTGGCCCTGAACAAGTTCGAG AAGGAGAGCGGCCACATCCGCAACCAGCGCGGCGACTACAGCCACACCTTCAGCCGCAAGGA CCTGCAGGCCGAGCTGATCCTGCTGTTCGAGAAGCAGAAGGAGTTCGGCAACCCCCACGTGA GCGGCGGCCTGAAGGAGGGCATCGAGACCCTGCTGATGACCCAGCGCCCCGCCCTGAGCGGC GACGCCGTGCAGAAGATGCTGGGCCACTGCACCTTCGAGCCAGCCGAGCCCAAGGCCGCCAA GAACACCTACACCGCCGAGCGCTTCATCTGGCTGACCAAGCTGAACAACCTGCGCATCCTGG AGCAGGGCAGCGAGCGCCCCCTGACCGACACCGAGCGCGCCACCCTGATGGACGAGCCCTAC CGCAAGAGCAAGCTGACCTACGCCCAGGCCCGCAAGCTGCTGGGTCTGGAGGACACCGCCTT CTTCAAGGGCCTGCGCTACGGCAAGGACAACGCCGAGGCCAGCACCCTGATGGAGATGAAGG CCTACCACGCCATCAGCCGCGCCCTGGAGAAGGAGGGCCTGAAGGACAAGAAGAGTCCTCTG AACCTGAGCCCCGAGCTGCAGGACGAGATCGGCACCGCCTTCAGCCTGTTCAAGACCGACGA GGACATCACCGGCCGCCTGAAGGACCGCATCCAGCCCGAGATCCTGGAGGCCCTGCTGAAGC ACATCAGCTTCGACAAGTTCGTGCAGATCAGCCTGAAGGCCCTGCGCCGCATCGTGCCCCTG ATGGAGCAGGGCAAGCGCTACGACGAGGCCTGCGCCGAGATCTACGGCGACCACTACGGCAA GAAGAACACCGAGGAGAAGATCTACCTGCCTCCTATCCCCGCCGACGAGATCCGCAACCCCG TGGTGCTGCGCGCCCTGAGCCAGGCCCGCAAGGTGATCAACGGCGTGGTGCGCCGCTACGGC AGCCCCGCCCGCATCCACATCGAGACCGCCCGCGAGGTGGGCAAGAGCTTCAAGGACCGCAA GGAGATCGAGAAGCGCCAGGAGGAGAACCGCAAGGACCGCGAGAAGGCCGCCGCCAAGTTCC GCGAGTACTTCCCCAACTTCGTGGGCGAGCCCAAGAGCAAGGACATCCTGAAGCTGCGCCTG TACGAGCAGCAGCACGGCAAGTGCCTGTACAGCGGCAAGGAGATCAACCTGGGCCGCCTGAA CGAGAAGGGCTACGTGGAGATCGACCACGCCCTGCCCTTCAGCCGCACCTGGGACGACAGCT TCAACAACAAGGTGCTGGTGCTGGGCAGCGAGAACCAGAACAAGGGCAACCAGACCCCCTAC GAGTACTTCAACGGCAAGGACAACAGCCGCGAGTGGCAGGAGTTCAAGGCCCGCGTGGAGAC CAGCCGCTTCCCCCGCAGCAAGAAGCAGCGCATCCTGCTGCAGAAGTTCGACGAGGACGGCT TCAAGGAGCGCAACCTGAACGACACCCGCTACGTGAACCGCTTCCTGTGCCAGTTCGTGGCC GACCGCATGCGCCTGACCGGCAAGGGCAAGAAGCGCGTGTTCGCCAGCAACGGCCAGATCAC CAACCTGCTGCGCGGCTTCTGGGGCCTGCGCAAGGTGCGCGCCGAGAACGACCGCCACCACG CCCTGGACGCCGTGGTGGTGGCCTGCAGCACCGTGGCCATGCAGCAGAAGATCACCCGCTTC GTGCGCTACAAGGAGATGAACGCCTTCGACGGTAAAACCATCGACAAGGAGACCGGCGAGGT GCTGCACCAGAAGACCCACTTCCCCCAGCCCTGGGAGTTCTTCGCCCAGGAGGTGATGATCC GCGTGTTCGGCAAGCCCGACGGCAAGCCCGAGTTCGAGGAGGCCGACACCCCCGAGAAGCTG CGCACCCTGCTGGCCGAGAAGCTGAGCAGCCGCCCTGAGGCCGTGCACGAGTACGTGACTCC TCTGTTCGTGAGCCGCGCCCCCAACCGCAAGATGAGCGGTCAGGGTCACATGGAGACCGTGA AGAGCGCCAAGCGCCTGGACGAGGGCGTGAGCGTGCTGCGCGTGCCCCTGACCCAGCTGAAG CTGAAGGACCTGGAGAAGATGGTGAACCGCGAGCGCGAGCCCAAGCTGTACGAGGCCCTGAA GGCCCGCCTGGAGGCCCACAAGGACGACCCCGCCAAGGCCTTCGCCGAGCCCTTCTACAAGT ACGACAAGGCCGGCAACCGCACCCAGCAGGTGAAGGCCGTGCGCGTGGAGCAGGTGCAGAAG ACCGGCGTGTGGGTGCGCAACCACAACGGCATCGCCGACAACGCCACCATGGTGCGCGTGGA CGTGTTCGAGAAGGGCGACAAGTACTACCTGGTGCCCATCTACAGCTGGCAGGTGGCCAAGG GCATCCTGCCCGACCGCGCCGTGGTGCAGGGCAAGGACGAGGAGGACTGGCAGCTGATCGAC GACAGCTTCAACTTCAAGTTCAGCCTGCACCCCAACGACCTGGTGGAGGTGATCACCAAGAA GGCCCGCATGTTCGGCTACTTCGCCAGCTGCCACCGCGGCACCGGCAACATCAACATCCGCA TCCACGACCTGGACCACAAGATCGGCAAGAACGGCATCCTGGAGGGCATCGGCGTGAAGACC GCCCTGAGCTTCCAGAAGTACCAGATCGACGAGCTGGGCAAGGAGATCCGCCCCTGCCGCCT GAAGAAGCGCCCTCCTGTGCGCTAA

(SEQ ID NO: 24)

Provided below is the corresponding amino acid sequence of a N. meningitidisCas9 molecule.

MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVRVFERAEVPKTGDSLAMAR RLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIKSLPNTPWQLRAAALDRKLTP LEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVADNAHALQTGDFRTPAELALNKFE KESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSG DAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERATLMDEPY RKSKLTYAQARKLLGLEDTAFFKGLRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPL NLSPELQDEIGTAFSLFKTDEDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPL MEQGKRYDEACAEIYGDHYGKKNTEEKIYLPPIPADEIRNPWLRALSQARKVINGWRRYG SPARIHIETAREVGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRL YEQQHGKCLYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPY EYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAWVACSTVAMQQKITRF VRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKL RTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLK LKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAWQGKDEEDWQLID DSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEGIGVKT ALSFQKYQIDELGKEIRPCRLKKRPPVR*

(SEQ ID NO: 25)

Provided below is an amino acid sequence of a S. aureus Cas9 molecule.

MKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRH RIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNVNEV EEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKV QKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVK YAYNADLYNALNDLNNLVITRDENEKLEYYEKFQI IENVFKQKKKPTLKQIAKEILVNEEDI KGYRVTSTGKPEFTNLKVYHDIKDITARKEI IENAELLDQIAKILTIYQSSEDIQEELTNLN SELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQK EIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQK RNRQTNERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHI IPRSVSFDNSFNNKVLVKQEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISK TKKEYLLEERDINRFSVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTS FLRRKWKFKKERNKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEI ETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLN GLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKY SKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKWKLSLKPYRFDVYLDNGVYKFVTVKN LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNRI EVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIKKG* (SEQ ID NO: 26)

Provided below is an exemplary codon optimized nucleic acid sequence encoding a

Cas9 molecule of S. aureus Cas9.

ATGAAAAGGAACTACATTCTGGGGCTGGACATCGGGATTACAAGCGTGGGGTATGGGATTAT TGACTATGAAACAAGGGACGTGATCGACGCAGGCGTCAGACTGTTCAAGGAGGCCAACGTGG AAAACAATGAGGGACGGAGAAGCAAGAGGGGAGCCAGGCGCCTGAAACGACGGAGAAGGCAC AGAATCCAGAGGGTGAAGAAACTGCTGTTCGATTACAACCTGCTGACCGACCATTCTGAGCT GAGTGGAATTAATCCTTATGAAGCCAGGGTGAAAGGCCTGAGTCAGAAGCTGTCAGAGGAAG AGTTTTCCGCAGCTCTGCTGCACCTGGCTAAGCGCCGAGGAGTGCATAACGTCAATGAGGTG GAAGAGGACACCGGCAACGAGCTGTCTACAAAGGAACAGATCTCACGCAATAGCAAAGCTCT GGAAGAGAAGTATGTCGCAGAGCTGCAGCTGGAACGGCTGAAGAAAGATGGCGAGGTGAGAG GGTCAATTAATAGGTTCAAGACAAGCGACTACGTCAAAGAAGCCAAGCAGCTGCTGAAAGTG CAGAAGGCTTACCACCAGCTGGATCAGAGCTTCATCGATACTTATATCGACCTGCTGGAGAC TCGGAGAACCTACTATGAGGGACCAGGAGAAGGGAGCCCCTTCGGATGGAAAGACATCAAGG AATGGTACGAGATGCTGATGGGACATTGCACCTATTTTCCAGAAGAGCTGAGAAGCGTCAAG TACGCTTATAACGCAGATCTGTACAACGCCCTGAATGACCTGAACAACCTGGTCATCACCAG GGATGAAAACGAGAAACTGGAATACTATGAGAAGTTCCAGATCATCGAAAACGTGTTTAAGC AGAAGAAAAAGCCTACACTGAAACAGATTGCTAAGGAGATCCTGGTCAACGAAGAGGACATC AAGGGCTACCGGGTGACAAGCACTGGAAAACCAGAGTTCACCAATCTGAAAGTGTATCACGA TATTAAGGACATCACAGCACGGAAAGAAATCATTGAGAACGCCGAACTGCTGGATCAGATTG CTAAGATCCTGACTATCTACCAGAGCTCCGAGGACATCCAGGAAGAGCTGACTAACCTGAAC AGCGAGCTGACCCAGGAAGAGATCGAACAGATTAGTAATCTGAAGGGGTACACCGGAACACA CAACCTGTCCCTGAAAGCTATCAATCTGATTCTGGATGAGCTGTGGCATACAAACGACAATC AGATTGCAATCTTTAACCGGCTGAAGCTGGTCCCAAAAAAGGTGGACCTGAGTCAGCAGAAA GAGATCCCAACCACACTGGTGGACGATTTCATTCTGTCACCCGTGGTCAAGCGGAGCTTCAT CCAGAGCATCAAAGTGATCAACGCCATCATCAAGAAGTACGGCCTGCCCAATGATATCATTA TCGAGCTGGCTAGGGAGAAGAACAGCAAGGACGCACAGAAGATGATCAATGAGATGCAGAAA CGAAACCGGCAGACCAATGAACGCATTGAAGAGATTATCCGAACTACCGGGAAAGAGAACGC AAAGTACCTGATTGAAAAAATCAAGCTGCACGATATGCAGGAGGGAAAGTGTCTGTATTCTC TGGAGGCCATCCCCCTGGAGGACCTGCTGAACAATCCATTCAACTACGAGGTCGATCATATT ATCCCCAGAAGCGTGTCCTTCGACAATTCCTTTAACAACAAGGTGCTGGTCAAGCAGGAAGA GAACTCTAAAAAGGGCAATAGGACTCCTTTCCAGTACCTGTCTAGTTCAGATTCCAAGATCT CTTACGAAACCTTTAAAAAGCACATTCTGAATCTGGCCAAAGGAAAGGGCCGCATCAGCAAG ACCAAAAAGGAGTACCTGCTGGAAGAGCGGGACATCAACAGATTCTCCGTCCAGAAGGATTT TATTAACCGGAATCTGGTGGACACAAGATACGCTACTCGCGGCCTGATGAATCTGCTGCGAT CCTATTTCCGGGTGAACAATCTGGATGTGAAAGTCAAGTCCATCAACGGCGGGTTCACATCT TTTCTGAGGCGCAAATGGAAGTTTAAAAAGGAGCGCAACAAAGGGTACAAGCACCATGCCGA AGATGCTCTGATTATCGCAAATGCCGACTTCATCTTTAAGGAGTGGAAAAAGCTGGACAAAG CCAAGAAAGTGATGGAGAACCAGATGTTCGAAGAGAAGCAGGCCGAATCTATGCCCGAAATC GAGACAGAACAGGAGTACAAGGAGATTTTCATCACTCCTCACCAGATCAAGCATATCAAGGA TTTCAAGGACTACAAGTACTCTCACCGGGTGGATAAAAAGCCCAACAGAGAGCTGATCAATG ACACCCTGTATAGTACAAGAAAAGACGATAAGGGGAATACCCTGATTGTGAACAATCTGAAC GGACTGTACGACAAAGATAATGACAAGCTGAAAAAGCTGATCAACAAAAGTCCCGAGAAGCT GCTGATGTACCACCATGATCCTCAGACATATCAGAAACTGAAGCTGATTATGGAGCAGTACG GCGACGAGAAGAACCCACTGTATAAGTACTATGAAGAGACTGGGAACTACCTGACCAAGTAT AGCAAAAAGGATAATGGCCCCGTGATCAAGAAGATCAAGTACTATGGGAACAAGCTGAATGC CCATCTGGACATCACAGACGATTACCCTAACAGTCGCAACAAGGTGGTCAAGCTGTCACTGA AGCCATACAGATTCGATGTCTATCTGGACAACGGCGTGTATAAATTTGTGACTGTCAAGAAT CTGGATGTCATCAAAAAGGAGAACTACTATGAAGTGAATAGCAAGTGCTACGAAGAGGCTAA AAAGCTGAAAAAGATTAGCAACCAGGCAGAGTTCATCGCCTCCTTTTACAACAACGACCTGA TTAAGATCAATGGCGAACTGTATAGGGTCATCGGGGTGAACAATGATCTGCTGAACCGCATT GAAGTGAATATGATTGACATCACTTACCGAGAGTATCTGGAAAACATGAATGATAAGCGCCC CCCTCGAATTATCAAAACAATTGCCTCTAAGACTCAGAGTATCAAAAAGTACTCAACCGACA TTCTGGGAAACCTGTATGAGGTGAAGAGCAAAAAGCACCCTCAGATTATCAAAAAGGGC

(SEQ ID NO: 39)

If any of the above Cas9 sequences are fused with a peptide or polypeptide at the C- terminus, it is understood that the stop codon will be removed.

Other Cas Molecules and Cas9 polypeptides

Various types of Cas molecules or Cas9 polypeptides can be used to practice the inventions disclosed herein. In some embodiments, Cas molecules of Type II Cas systems are used. In other embodiments, Cas molecules of other Cas systems are used. For example Type I or Type III Cas molecules may be used. Exemplary Cas molecules (and Cas systems) are described, e.g., in Haft et al, PLoS COMPUTATIONAL BIOLOGY 2005, 1(6): e60 and Makarova et al, NATURE REVIEW MICROBIOLOGY 2011, 9:467-477, the contents of both references are incorporated herein by reference in their entirety. Exemplary Cas molecules (and Cas systems) are also shown in Table 22.

Table 22: Cas Systems

Gene System type Name from Structure of Families (and Representatives name* or subtype Haft et al.^% encoded superfamily) of

protein (PDB encoded

accessions)^*" protein*^**

cas8b • Subtype I- cshl and NA BH0338-like MTH1090 and

B** TM1802 TM1802 cas8c • Subtype I- csdl and csp2 NA BH0338-like BH0338

C**

cas9 • Type II** csnl and csxl2 NA COG3513 FTN_0757 and

SPyl046 cas 10 • Type III** cmr2, csml NA COG1353 MTH326, Rv2823c^§§ and csxll and TM1794^§§ cas 10 d • Subtype I- csc3 NA COG1353 slr7011

D**

csyl • Subtype I- csyl NA yl724-like yl724

F**

csy2 • Subtype I-F csy2 NA (RAMP) yl725

csy3 • Subtype I-F csy3 NA (RAMP) yl726

csel • Subtype I- csel NA YgcL-like ygcL

E**

cse2 • Subtype I-E cse2 2ZCA YgcK-like ygcK

cscl • Subtype I-D cscl NA air 1563 -like alrl563

(RAMP)

csc2 • Subtype I-D cscl and csc2 NA COG1337 slr7012

(RAMP)

csa5 • Subtype I-A csa5 NA AF1870 AF1870, MJ0380,

PF0643 and SS01398 csn2 • Subtype II- csn2 NA SPyl049-like SPyl049

A

csm2 • Subtype III- csm2 NA COG1421 MTH1081 and

A** SERP2460 csm3 • Subtype III- csc2 and csm3 NA COG1337 MTH1080 and A (RAMP) SERP2459 csm4 • Subtype III- csm4 NA COG1567 MTH1079 and A (RAMP) SERP2458 csm5 • Subtype III- csm5 NA COG1332 MTH1078 and A (RAMP) SERP2457 csm6 • Subtype III- APE2256 and 2WTE COG1517 APE2256 and

A csm6 SS01445 cmrl • Subtype III- cmrl NA COG1367 PF1130

B (RAMP)

cmr3 • Subtype Hi- cmr3 NA COG1769 PF1128

fi (RAMP)

cmr4 • Subtype III- cmr4 NA COG1336 PF1126

B (RAMP)

cmr5 • Subtype Ill- cmr5 2ZOP and COG3337 MTH324 and PF1125 Table 22: Cas Systems

Gene System type Name from Structure of Families (and Representatives name* or subtype Haft et al.^% encoded superfamily) of

protein (PDB encoded

accessions)^*" protein*^**

B** 20EB

cmr6 • Subtype III- cmr6 NA COG1604 PF1124

B (RAMP)

csbl • Subtype I-U GSU0053 NA (RAMP) Balac_1306 and

GSU0053 csbl • Subtype I- NA NA (RAMP) Balac_1305 and

GSU0054 csb3 • Subtype I-U NA NA (RAMP) Balac_1303^§§ csxl7 • Subtype I-U NA NA NA Btus_2683 csxl4 • Subtype I-U NA NA NA GSU0052 csxlO • Subtype I-U csxlO NA (RAMP) Caur_2274 csxl6 • Subtype III- VVA1548 NA NA VVA1548

U

csaX • Subtype III- csaX NA NA SS01438

U

csx3 • Subtype III- csx3 NA NA AF1864

U

csxl • Subtype III- csa3, csxl, 1XMX and 2171 COG1517 and MJ1666, NE0113, U csx2, DXTHG, COG4006 PF1127 and TM1812

NE0113 and

TIG 02710

csxl5 • Unknown NA NA TTE2665 TTE2665 csfl • Type U csfl NA NA AFE_1038 csfl • Type U csfl NA (RAMP) AFE_1039 csfl • Type U csfl NA (RAMP) AFE_1040 csfl • Type U csfl NA NA AFE_1037

IV. Functional Analysis of Candidate Molecules

Candidate Cas9 molecules, candidate gRNA molecules, candidate Cas9

molecule/gRNA molecule complexes, can be evaluated by art-known methods or as described herein. For example, exemplary methods for evaluating the endonuclease activity of Cas9 molecule are described, e.g., in Jinek et ah, SCIENCE 2012, 337(6096):816-821.

Binding and Cleavage Assay: Testing the endonuclease activity of Cas9 molecule The ability of a Cas9 molecule/gRNA molecule complex to bind to and cleave a target nucleic acid can be evaluated in a plasmid cleavage assay. In this assay, synthetic or in vitro- transcribed gRNA molecule is pre-annealed prior to the reaction by heating to 95 °C and slowly cooling down to room temperature. Native or restriction digest- linearized plasmid DNA (300 ng (~8 nM)) is incubated for 60 min at 37°C with purified Cas9 protein molecule (50-500 nM) and gRNA (50-500 nM, 1:1) in a Cas9 plasmid cleavage buffer (20 mM HEPES pH 7.5, 150 mM KCl, 0.5 mM DTT, 0.1 mM EDTA) with or without 10 mM MgCl₂. The reactions are stopped with 5X DNA loading buffer (30% glycerol, 1.2% SDS, 250 mM EDTA), resolved by a 0.8 or 1% agarose gel electrophoresis and visualized by ethidium bromide staining. The resulting cleavage products indicate whether the Cas9 molecule cleaves both DNA strands, or only one of the two strands. For example, linear DNA products indicate the cleavage of both DNA strands. Nicked open circular products indicate that only one of the two strands is cleaved.

Alternatively, the ability of a Cas9 molecule/gRNA molecule complex to bind to and cleave a target nucleic acid can be evaluated in an oligonucleotide DNA cleavage assay. In this assay, DNA oligonucleotides (10 pmol) are radiolabeled by incubating with 5 units T4 polynucleotide kinase and -3-6 pmol (-20^40 mCi) [γ-32Ρ]-ΑΤΡ in IX T4 polynucleotide kinase reaction buffer at 37°C for 30 min, in a 50 μΕ reaction. After heat inactivation (65°C for 20 min), reactions are purified through a column to remove unincorporated label. Duplex substrates (100 nM) are generated by annealing labeled oligonucleotides with equimolar amounts of unlabeled complementary oligonucleotide at 95°C for 3 min, followed by slow cooling to room temperature. For cleavage assays, gRNA molecules are annealed by heating to 95 °C for 30 s, followed by slow cooling to room temperature. Cas9 (500 nM final concentration) is pre-incubated with the annealed gRNA molecules (500 nM) in cleavage assay buffer (20 mM HEPES pH 7.5, 100 mM KCl, 5 mM MgC12, 1 mM DTT, 5% glycerol) in a total volume of 9 μΐ. Reactions are initiated by the addition of 1 μΐ target DNA (10 nM) and incubated for 1 h at 37 °C. Reactions are quenched by the addition of 20 μΐ of loading dye (5 mM EDTA, 0.025% SDS, 5% glycerol in formamide) and heated to 95°C for 5 min. Cleavage products are resolved on 12% denaturing polyacrylamide gels containing 7 M urea and visualized by phosphorimaging. The resulting cleavage products indicate that whether the complementary strand, the non-complementary strand, or both, are cleaved.

One or both of these assays can be used to evaluate the suitability of a candidate gRNA molecule or candidate Cas9 molecule.

Binding Assay: Testing the binding of Cas9 molecule to target DNA

Exemplary methods for evaluating the binding of Cas9 molecule to target DNA are described, e.g., in Jinek et al, SCIENCE 2012; 337(6096):816-821. For example, in an electrophoretic mobility shift assay, target DNA duplexes are formed by mixing of each strand (10 nmol) in deionized water, heating to 95 °C for 3 min and slow cooling to room temperature. All DNAs are purified on 8% native gels containing IX TBE. DNA bands are visualized by UV shadowing, excised, and eluted by soaking gel pieces in DEPC-treated H₂0. Eluted DNA is ethanol precipitated and dissolved in DEPC- treated H₂0. DNA samples are 5' end labeled with [γ-32Ρ]-ΑΤΡ using T4 polynucleotide kinase for 30 min at 37°C. Polynucleotide kinase is heat denatured at 65°C for 20 min, and unincorporated radiolabel is removed using a column. Binding assays are performed in buffer containing 20 mM HEPES pH 7.5, 100 mM KC1, 5 mM MgCl₂, 1 mM DTT and 10% glycerol in a total volume of 10 μΐ. Cas9 protein molecule is programmed with equimolar amounts of pre-annealed gRNA molecule and titrated from 100 pM to 1 μΜ. Radiolabeled DNA is added to a final concentration of 20 pM. Samples are incubated for 1 h at 37°C and resolved at 4°C on an 8% native polyacrylamide gel containing IX TBE and 5 mM MgCl₂. Gels are dried and DNA visualized by phosphorimaging.

Differential Scanning Flourimetry (DSF)

The thermostability of Cas9-gRNA ribonucleoprotein (RNP) complexes can be measured via DSF. This technique measures the thermostability of a protein, which can increase under favorable conditions such as the addition of a binding RNA molecule, e.g., a gRNA.

The assay is performed using two different protocols, one to test the best

stoichiometric ratio of gRNA:Cas9 protein and another to determine the best solution conditions for RNP formation.

To determine the best solution to form RNP complexes, a 2uM solution of Cas9 in water+lOx SYPRO Orange® (Life Techonologies cat#S-6650) and dispensed into a 384 well plate. An equimolar amount of gRNA diluted in solutions with varied pH and salt is then added. After incubating at room temperature for 10' and brief centrifugation to remove any bubbles,a Bio-Rad CFX384™ Real-Time System CIOOO Touch™ Thermal Cycler with the Bio-Rad CFX Manager software is used to run a gradient from 20°C to 90°C with a 1° increase in temperature every lOseconds.

The second assay consists of mixing various concentrations of gRNA with 2uM Cas9 in optimal buffer from assay 1 above and incubating at RT for 10' in a 384 well plate. An equal volume of optimal buffer + lOx SYPRO Orange® (Life Techonologies cat#S-6650) is added and the plate sealed with Microseal® B adhesive (MSB- 1001). Following brief centrifugation to remove any bubbles, a Bio-Rad CFX384™ Real-Time System CIOOO Touch™ Thermal Cycler with the Bio-Rad CFX Manager software is used to run a gradient from 20°C to 90°C with a 1° increase in temperature every lOseconds. V. Genome Editing Approaches

Described herein are methods for targeted knockout of one or more copies (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more copies) of the UL19, UL30, UL48 and/or UL54 gene, e.g., using one or more of the approaches or pathways described herein, e.g., using NHEJ.

V.l NHEJ Approaches for Gene Targeting

As described herein, nuclease-induced non-homologous end-joining (NHEJ) can be used to target gene-specific knockouts. Nuclease-induced NHEJ can also be used to remove (e.g., delete) sequence (e.g., coding sequence, non-coding sequence, or sequence insertions) in a gene of interest.

While not wishing to be bound by theory, it is believed that, in an embodiment, the genomic alterations associated with the methods described herein rely on nuclease-induced NHEJ and the error-prone nature of the NHEJ repair pathway. NHEJ repairs a double-strand break in the DNA by joining together the two ends; however, generally, the original sequence is restored only if two compatible ends, exactly as they were formed by the double- strand break, are perfectly ligated. The DNA ends of the double-strand break are frequently the subject of enzymatic processing, resulting in the addition or removal of nucleotides, at one or both strands, prior to rejoining of the ends. This results in the presence of insertion and/or deletion (indel) mutations in the DNA sequence at the site of the NHEJ repair. Two-thirds of these mutations typically alter the reading frame and, therefore, produce a non-functional protein. Additionally, mutations that maintain the reading frame, but which insert or delete a significant amount of sequence, can destroy functionality of the protein. This is locus dependent as mutations in critical functional domains are likely less tolerable than mutations in non-critical regions of the protein.

The indel mutations generated by NHEJ are unpredictable in nature; however, at a given break site certain indel sequences are favored and are over represented in the population, likely due to small regions of microhomology. The lengths of deletions can vary widely; most commonly in the 1-50 bp range, but they can easily reach greater than 100-200 bp. Insertions tend to be shorter and often include short duplications of the sequence immediately surrounding the break site. However, it is possible to obtain large insertions, and in these cases, the inserted sequence has often been traced to other regions of the genome or to plasmid DNA present in the cells.

Because NHEJ is a mutagenic process, it can also be used to delete small sequence motifs as long as the generation of a specific final sequence is not required. If a double- strand break is targeted near to a short target sequence, the deletion mutations caused by the NHEJ repair often span, and therefore remove, the unwanted nucleotides. For the deletion of larger DNA segments, introducing two double-strand breaks, one on each side of the sequence, can result in NHEJ between the ends with removal of the entire intervening sequence. Both of these approaches can be used to delete specific DNA sequences; however, the error-prone nature of NHEJ may still produce indel mutations at the site of repair.

Both double strand cleaving eaCas9 molecules and single strand, or nickase, eaCas9 molecules can be used in the methods and compositions described herein to generate NHEJ- mediated indels. NHEJ-mediated indels targeted to the the gene, e.g., a coding region, e.g., an early coding region of a gene, of interest can be used to knockout (i.e., eliminate expression of) a gene of interest. For example, early coding region of a gene of interest includes sequence immediately following a transcription start site, within a first exon of the coding sequence, or within 500 bp of the transcription start site (e.g., less than 500, 450, 400, 350, 300, 250, 200, 150, 100 or 50 bp).

In some embodiments, NHEJ-mediated indels are introduced into more than one HSV-2 target gene. In an embodiment, in which Cas9 double- stranded nuclease or Cas9 single- stranded nickase is used to introduce mutations into two HSV-2 genes, e.g., UL19 and UL30, UL19 and UL48, UL19 and UL54, UL30 and UL48, UL30 and UL54, or UL48 and UL54, individual gRNAs or gRNA pairs targeting both genes are provided together with Cas9 double- stranded nuclease or single- stranded nickase. In an embodiment, in which Cas9 double- stranded nuclease or Cas9 single-stranded nickase is used to introduce mutations into three HSV-2 genes, e.g., UL19 and UL30 and UL48, or UL19 and UL30 and UL54, UL19 and UL48 and UL54, or UL30 and UL48 and UL54, individual gRNAs or gRNA pairs targeting all three genes are provided together with Cas9 double- stranded nuclease or single- stranded nickase. In an embodiment, in which Cas9 double-stranded nuclease or Cas9 single- stranded nickase is used to introduce mutations into four HSV-2 genes, e.g., UL19 and UL30 and UL48 and UL54, individual gRNAs or gRNA pairs targeting all four genes are provided together with Cas9 double-stranded nuclease or single-stranded nickase.

Placement of double strand or single strand breaks relative to the target position In an embodiment, in which a gRNA and Cas9 nuclease generate a double strand break for the purpose of inducing NHEJ-mediated indels, a gRNA, e.g., a unimolecular (or chimeric) or modular gRNA molecule, is configured to position one double-strand break in close proximity to a nucleotide of the target position, e.g., a HSV-2 target UL19 position, a HSV-2 target UL30 position, a HSV-2 target UL48 position or a HSV-2 target UL54 position. In an embodiment, the cleavage site is between 0-30 bp away from the target position (e.g., less than 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 bp from the target position).

In an embodiment, in which two gRNAs complexing with Cas9 nickases induce two single strand breaks for the purpose of inducing NHEJ-mediated indels, two gRNAs, e.g., independently, unimolecular (or chimeric) or modular gRNA, are configured to position two single-strand breaks to provide for NHEJ repair a nucleotide of the target position. In an embodiment, the gRNAs are configured to position cuts at the same position, or within a few nucleotides of one another, on different strands, essentially mimicking a double strand break. In an embodiment, the closer nick is between 0-30 bp away from the target position (e.g., less than 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 bp from the target position), and the two nicks are within 25-55 bp of each other (e.g., between 25 to 50, 25 to 45, 25 to 40, 25 to 35, 25 to 30, 50 to 55, 45 to 55, 40 to 55, 35 to 55, 30 to 55, 30 to 50, 35 to 50, 40 to 50 , 45 to 50, 35 to 45, or 40 to 45 bp) and no more than 100 bp away from each other (e.g., no more than 90, 80, 70, 60, 50, 40, 30, 20 or 10 bp). In an embodiment, the gRNAs are configured to place a single strand break on either side of a nucleotide of the target position.

Both double strand cleaving eaCas9 molecules and single strand, or nickase, eaCas9 molecules can be used in the methods and compositions described herein to generate breaks both sides of a target position. Double strand or paired single strand breaks may be generated on both sides of a target position to remove the nucleic acid sequence between the two cuts (e.g., the region between the two breaks in deleted). In one embodiment, two gRNAs, e.g., independently, unimolecular (or chimeric) or modular gRNA, are configured to position a double-strand break on both sides of a target position. In an alternate embodiment, three gRNAs, e.g., independently, unimolecular (or chimeric) or modular gRNA, are configured to position a double strand break (i.e., one gRNA complexes with a cas9 nuclease) and two single strand breaks or paired single stranded breaks (i.e., two gRNAs complex with Cas9 nickases) on either side of the target position. In another embodiment, four gRNAs, e.g., independently, unimolecular (or chimeric) or modular gRNA, are configured to generate two pairs of single stranded breaks (i.e., two pairs of two gRNAs complex with Cas9 nickases) on either side of the target position. The double strand break(s) or the closer of the two single strand nicks in a pair will ideally be within 0-500 bp of the target position (e.g., no more than 450, 400, 350, 300, 250, 200, 150, 100, 50 or 25 bp from the target position). When nickases are used, the two nicks in a pair are within 25-55 bp of each other (e.g., between 25 to 50, 25 to 45, 25 to 40, 25 to 35, 25 to 30, 50 to 55, 45 to 55, 40 to 55, 35 to 55, 30 to 55, 30 to 50, 35 to 50, 40 to 50 , 45 to 50, 35 to 45, or 40 to 45 bp) and no more than 100 bp away from each other (e.g., no more than 90, 80, 70, 60, 50, 40, 30, 20 or 10 bp).

V.2 Single-Strand Annealing

Single strand annealing (SSA) is another DNA repair process that repairs a double- strand break between two repeat sequences present in a target nucleic acid. Repeat sequences utilized by the SSA pathway are generally greater than 30 nucleotides in length. Resection at the break ends occurs to reveal repeat sequences on both strands of the target nucleic acid. After resection, single strand overhangs containing the repeat sequences are coated with RPA protein to prevent the repeats sequences from inappropriate annealing, e.g., to themselves. RAD52 binds to and each of the repeat sequences on the overhangs and aligns the sequences to enable the annealing of the complementary repeat sequences. After annealing, the single- strand flaps of the overhangs are cleaved. New DNA synthesis fills in any gaps, and ligation restores the DNA duplex. As a result of the processing, the DNA sequence between the two repeats is deleted. The length of the deletion can depend on many factors including the location of the two repeats utilized, and the pathway or processivity of the resection.

In contrast to HDR pathways, SSA does not require a template nucleic acid to alter or correct a target nucleic acid sequence. Instead, the complementary repeat sequence is utilized. V. 3 Other DNA Repair Pathways

SSBR (single strand break repair)

Single- stranded breaks (SSB) in the genome are repaired by the SSBR pathway, which is a distinct mechanism from the DSB repair mechanisms discussed above. The SSBR pathway has four major stages: SSB detection, DNA end processing, DNA gap filling, and DNA ligation. A more detailed explanation is given in Caldecott, Nature Reviews Genetics 9, 619-631 (August 2008), and a summary is given here.

In the first stage, when a SSB forms, PARP1 and/or PARP2 recognize the break and recruit repair machinery. The binding and activity of PARP1 at DNA breaks is transient and it seems to accelerate SSBr by promoting the focal accumulation or stability of SSBr protein complexes at the lesion. Arguably the most important of these SSBr proteins is XRCC1, which functions as a molecular scaffold that interacts with, stabilizes, and stimulates multiple enzymatic components of the SSBr process including the protein responsible for cleaning the DNA 3' and 5' ends. For instance, XRCC1 interacts with several proteins (DNA polymerase beta, PNK, and three nucleases, APE1, APTX, and APLF) that promote end processing. APE1 has endonuc lease activity. APLF exhibits endonuclease and 3' to 5' exonuclease activities. APTX has endonuclease and 3' to 5' exonuclease activity.

This end processing is an important stage of SSBR since the 3' - and/or 5 '-termini of most, if not all, SSBs are 'damaged'. End processing generally involves restoring a damaged 3 '-end to a hydroxylated state and and/or a damaged 5' end to a phosphate moiety, so that the ends become ligation-competent. Enzymes that can process damaged 3 ' termini include PNKP, APE1, and TDP1. Enzymes that can process damaged 5' termini include PNKP, DNA polymerase beta, and APTX. LIG3 (DNA ligase III) can also participate in end processing. Once the ends are cleaned, gap filling can occur.

At the DNA gap filling stage, the proteins typically present are PARP1, DNA polymerase beta, XRCC1, FEN1 (flap endonculease 1), DNA polymerase delta/epsilon, PCNA, and LIG1. There are two ways of gap filling, the short patch repair and the long patch repair. Short patch repair involves the insertion of a single nucleotide that is missing. At some SSBs, "gap filling" might continue displacing two or more nucleotides

(displacement of up to 12 bases have been reported). FENl is an endonuclease that removes the displaced 5 '-residues. Multiple DNA polymerases, including Pol β , are involved in the repair of SSBs, with the choice of DNA polymerase influenced by the source and type of SSB.

In the fourth stage, a DNA ligase such as LIG1 (Ligase I) or LIG3 (Ligase III) catalyzes joining of the ends. Short patch repair uses Ligase III and long patch repair uses Ligase I.

Sometimes, SSBR is replication-coupled. This pathway can involve one or more of CtIP, MRN, ERCC1, and FENl. Additional factors that may promote SSBR include:

aPARP, PARP1, PARP2, PARG, XRCC1, DNA polymerase b, DNA polymerase d, DNA polymerase e, PCNA, LIG1, PNK, PNKP, APE1, APTX, APLF, TDP1, LIG3, FENl, CtIP, MRN, and ERCC1.

MMR (mismatch repair) Cells contain three excision repair pathways: MMR, BER, and NER. The excision repair pathways hace a common feature in that they typically recognize a lesion on one strand of the DNA, then exo/endonucleaseases remove the lesion and leave a 1-30 nucleotide gap that is sub- sequentially filled in by DNA polymerase and finally sealed with ligase. A more complete picture is given in Li, Cell Research (2008) 18:85-98, and a summary is provided here.

Mismatch repair (MMR) operates on mispaired DNA bases.

The MSH2/6 or MSH2/3 complexes both have ATPases activity that plays an important role in mismatch recognition and the initiation of repair. MSH2/6 preferentially recognizes base-base mismatches and identifies mispairs of 1 or 2 nucleotides, while MSH2/3 preferentially recognizes larger ID mispairs.

hMLHl heterodimerizes with hPMS2 to form hMutL which possesses an ATPase activity and is important for multiple steps of MMR. It possesses a PCNA/replication factor C (RFC)-dependent endonuclease activity which plays an important role in 3' nick-directed MMR involving EXOl. (EXOl is a participant in both HR and MMR.) It regulates termination of mismatch-provoked excision. Ligase I is the relevant ligase for this pathway. Additional factors that may promote MMR include: EXOl, MSH2, MSH3, MSH6, MLH1, PMS2, MLH3, DNA Pol d, RPA, HMGB1, RFC, and DNA ligase I. Base excision repair (BER)

The base excision repair (BER) pathway is active throughout the cell cycle; it is responsible primarily for removing small, non-helix-distorting base lesions from the genome. In contrast, the related Nucleotide Excision Repair pathway (discussed in the next section) repairs bulky helix-distorting lesions. A more detailed explanation is given in Caldecott, Nature Reviews Genetics 9, 619-631 (August 2008), and a summary is given here.

Upon DNA base damage, base excision repair (BER) is initiated and the process can be simplified into five major steps: (a) removal of the damaged DNA base; (b) incision of the subsequent a basic site; (c) clean-up of the DNA ends; (d) insertion of the correct nucleotide into the repair gap; and (e) ligation of the remaining nick in the DNA backbone. These last steps are similar to the SSBR.

In the first step, a damage-specific DNA glycosylase excises the damaged base through cleavage of the N-glycosidic bond linking the base to the sugar phosphate backbone. Then AP endonuclease- 1 (APE1) or bifunctional DNA glycosylases with an associated lyase activity incised the phosphodiester backbone to create a DNA single strand break (SSB). The third step of BER involves cleaning-up of the DNA ends. The fourth step in BER is conducted by Pol β that adds a new complementary nucleotide into the repair gap and in the final step XRCCl/Ligase III seals the remaining nick in the DNA backbone. This completes the short-patch BER pathway in which the majority (-80%) of damaged DNA bases are repaired. However, if the 5' -ends in step 3 are resistant to end processing activity, following one nucleotide insertion by Pol β there is then a polymerase switch to the replicative DNA polymerases, Pol δ/ε, which then add -2-8 more nucleotides into the DNA repair gap. This creates a 5' -flap structure, which is recognized and excised by flap endonuclease-1 (FEN-1) in association with the processivity factor proliferating cell nuclear antigen (PCNA). DNA ligase I then seals the remaining nick in the DNA backbone and completes long-patch BER. Additional factors that may promote the BER pathway include: DNA glycosylase, APE1, Polb, Pold, Pole, XRCC1, Ligase III, FEN-1, PCNA, RECQL4, WRN, MYH, PNKP, and APTX.

Nucleotide excision repair (NER)

Nucleotide excision repair (NER) is an important excision mechanism that removes bulky helix-distorting lesions from DNA. Additional details about NER are given in Marteijn et al., Nature Reviews Molecular Cell Biology 15, 465-481 (2014), and a summary is given here. NER a broad pathway encompassing two smaller pathways: global genomic NER (GG- NER) and transcription coupled repair NER (TC-NER). GG-NER and TC-NER use different factors for recognizing DNA damage. However, they utilize the same machinery for lesion incision, repair, and ligation.

Once damage is recognized, the cell removes a short single- stranded DNA segment that contains the lesion. Endonuc leases XPF/ERCC1 and XPG (encoded by ERCC5) remove the lesion by cutting the damaged strand on either side of the lesion, resulting in a single- strand gap of 22-30 nucleotides. Next, the cell performs DNA gap filling synthesis and ligation. Involved in this process are: PCNA, RFC, DNA Pol δ, DNA Pol ε or DNA Pol κ, and DNA ligase I or XRCCl/Ligase III. Replicating cells tend to use DNA pol ε and DNA ligase I, while non-replicating cells tend to use DNA Pol δ, DNA Pol κ, and the XRCC1/ Ligase III complex to perform the ligation step.

NER can involve the following factors: XPA-G, POLH, XPF, ERCC1, XPA-G, and LIG1. Transcription-coupled NER (TC-NER) can involve the following factors: CSA, CSB, XPB, XPD, XPG, ERCCl, and TTDA. Additional factors that may promote the NER repair pathway include XPA-G, POLH, XPF, ERCCl, XPA-G, LIG1, CSA, CSB, XPA, XPB, XPC, XPD, XPF, XPG, TTDA, UVSSA, USP7, CETN2, RAD23B, UV-DDB, CAK subcomplex, RPA, and PCNA.

Interstrand Crosslink (ICL)

A dedicated pathway called the ICL repair pathway repairs interstrand crosslinks. Interstrand crosslinks, or covalent crosslinks between bases in different DNA strand, can occur during replication or transcription. ICL repair involves the coordination of multiple repair processes, in particular, nucleolytic activity, translesion synthesis (TLS), and HDR. Nucleases are recruited to excise the ICL on either side of the crosslinked bases, while TLS and HDR are coordinated to repair the cut strands. ICL repair can involve the following factors: endonucleases, e.g., XPF and RAD51C, endonucleases such as RAD51, translesion polymerases, e.g., DNA polymerase zeta and Revl), and the Fanconi anemia (FA) proteins, e.g., FancJ.

Other pathways

Several other DNA repair pathways exist in mammals.

Translesion synthesis (TLS) is a pathway for repairing a single stranded break left after a defective replication event and involves translesion polymerases, e.g., DNA pol and Revl..

Error-free postreplication repair (PRR) is another pathway for repairing a single stranded break left after a defective replication event. V.4 Examples of gRNAs in Genome Editing Methods

gRNA molecules as described herein can be used with Cas9 molecules that generate a double strand break or a single strand break to alter the sequence of a target nucleic acid, e.g., a target position or target genetic signature. gRNA molecules useful in these methods are described below.

In an embodiment, the gRNA, e.g., a chimeric gRNA, is configured such that it comprises one or more of the following properties;

a) it can position, e.g., when targeting a Cas9 molecule that makes double strand breaks, a double strand break (i) within 50, 100, 150, 200, 250, 300, 350, 400, 450, or 500 nucleotides of a target position, or (ii) sufficiently close that the target position is within the region of end resection;

b) it has a targeting domain of at least 16 nucleotides, e.g., a targeting domain of (i) 16, (ii), 17, (iii) 18, (iv) 19, (v) 20, (vi) 21, (vii) 22, (viii) 23, (ix) 24, (x) 25, or (xi) 26 nucleotides; and

c)

(i) the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail and proximal domain, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(ii) there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S.

thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(iii) there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain, e.g., at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than l, 2, 3, 4, 5; 6, 7, 8, 9 or l0 nucleotides therefrom;

(iv) the tail domain is at least 10, 15, 20, 25, 30, 35 or 40 nucleotides in length, e.g., it comprises at least 10, 15, 20, 25, 30, 35 or 40 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom; or

(v) the tail domain comprises 15, 20, 25, 30, 35, 40 nucleotides or all of the corresponding portions of a naturally occurring tail domain, e.g., a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain.

In an embodiment, the gRNA is configured such that it comprises properties: a and In an embodiment, the gRNA is configured such that it comprises properties: a and b(ii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(iii).

In an embodiment, the gRNA is configured such that it comprises properties b(iv).

In an embodiment, the gRNA is configured such that it comprises properties b(v).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(vi).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(vii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(viii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(ix).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(x).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(xi).

In an embodiment, the gRNA is configured such that it comprises properties: a and c.

In an embodiment, the gRNA is configured such that in comprises properties: a, b, and c.

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(i), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(i), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(ii), and c(i). In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iv), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iv), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(v), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(v), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vi), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vi), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(viii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(viii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ix), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ix), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(x), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(x), and c(ii). In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(xi), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(xi), and c(ii).

In an embodiment, the gRNA, e.g., a chimeric gRNA, is configured such that it comprises one or more of the following properties;

a) one or both of the gRNAs can position, e.g., when targeting a Cas9 molecule that makes single strand breaks, a single strand break within (i) 50, 100, 150, 200, 250, 300, 350, 400, 450, or 500 nucleotides of a target position, or (ii) sufficiently close that the target position is within the region of end resection;

b) one or both have a targeting domain of at least 16 nucleotides, e.g., a targeting domain of (i) 16, (ii), 17, (iii) 18, (iv) 19, (v) 20, (vi) 21, (vii) 22, (viii) 23, (ix) 24, (x) 25, or (xi) 26 nucleotides; and

c)

(i) the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail and proximal domain, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(ii) there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S.

thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(iii) there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain, e.g., at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than l, 2, 3, 4, 5; 6, 7, 8, 9 or l0 nucleotides therefrom; (iv) the tail domain is at least 10, 15, 20, 25, 30, 35 or 40 nucleotides in length, e.g., it comprises at least 10, 15, 20, 25, 30, 35 or 40 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom; or

(v) the tail domain comprises 15, 20, 25, 30, 35, 40 nucleotides or all of the corresponding portions of a naturally occurring tail domain, e.g., a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain.

In an embodiment, the gRNA is configured such that it comprises properties: a and b(i).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(ii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(iii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(iv).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(v).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(vi).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(vii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(viii).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(ix).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(x).

In an embodiment, the gRNA is configured such that it comprises properties: a and b(xi).

In an embodiment, the gRNA is configured such that it comprises properties: a and c. In an embodiment, the gRNA is configured such that in comprises properties: a, b, and c. In an embodiment, the gRNA is configured such that in comprises properties: a(i b(i), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(i), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iv), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(iv), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(v), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(v), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vi), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vi), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(vii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(viii), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(viii), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i b(ix), and c(i). In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(ix), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(x), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(x), and c(ii).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(xi), and c(i).

In an embodiment, the gRNA is configured such that in comprises properties: a(i), b(xi), and c(ii).

In an embodiment, the gRNA is used with a Cas9 nickase molecule having HNH activity, e.g., a Cas9 molecule having the RuvC activity inactivated, e.g., a Cas9 molecule having a mutation at D10, e.g., the D10A mutation.

In an embodiment, the gRNA is used with a Cas9 nickase molecule having RuvC activity, e.g., a Cas9 molecule having the HNH activity inactivated, e.g., a Cas9 molecule having a mutation at 840, e.g., the H840A.

In an embodiment, the gRNAs are used with a Cas9 nickase molecule having RuvC activity, e.g., a Cas9 molecule having the HNH activity inactivated, e.g., a Cas9 molecule having a mutation at N863, e.g., the N863A mutation.

In an embodiment, a pair of gRNAs, e.g., a pair of chimeric gRNAs, comprising a first and a second gRNA, is configured such that they comprises one or more of the following properties;

a) one or both of the gRNAs can position, e.g., when targeting a Cas9 molecule that makes single strand breaks, a single strand break within (i) 50, 100, 150, 200, 250, 300, 350, 400, 450, or 500 nucleotides of a target position, or (ii) sufficiently close that the target position is within the region of end resection;

b) one or both have a targeting domain of at least 16 nucleotides, e.g., a targeting domain of (i) 16, (ii), 17, (iii) 18, (iv) 19, (v) 20, (vi) 21, (vii) 22, (viii) 23, (ix) 24, (x) 25, or (xi) 26 nucleotides;

c) for one or both:

(i) the proximal and tail domain, when taken together, comprise at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail and proximal domain, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(ii) there are at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides 3' to the last nucleotide of the second complementarity domain, e.g., at least 15, 18, 20, 25, 30, 31, 35, 40, 45, 49, 50, or 53 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S.

thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom;

(iii) there are at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides 3' to the last nucleotide of the second complementarity domain that is complementary to its corresponding nucleotide of the first complementarity domain, e.g., at least 16, 19, 21, 26, 31, 32, 36, 41, 46, 50, 51, or 54 nucleotides from the corresponding sequence of a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis gRNA, or a sequence that differs by no more than l, 2, 3, 4, 5; 6, 7, 8, 9 or l0 nucleotides therefrom;

(iv) the tail domain is at least 10, 15, 20, 25, 30, 35 or 40 nucleotides in length, e.g., it comprises at least 10, 15, 20, 25, 30, 35 or 40 nucleotides from a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain; or, or a sequence that differs by no more than 1, 2, 3, 4, 5; 6, 7, 8, 9 or 10 nucleotides therefrom; or

(v) the tail domain comprises 15, 20, 25, 30, 35, 40 nucleotides or all of the corresponding portions of a naturally occurring tail domain, e.g., a naturally occurring S. pyogenes, S. thermophilus, S. aureus, or N. meningitidis tail domain;

d) the gRNAs are configured such that, when hybridized to target nucleic acid, they are separated by 0-50, 0-100, 0-200, at least 10, at least 20, at least 30 or at least 50 nucleotides;

e) the breaks made by the first gRNA and second gRNA are on different strands; and f) the PAMs are facing outwards.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(ii). In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(iii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(iv).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(v).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(vi).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(vii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(viii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(ix).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(x).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a and b(xi).

In an embodiment, one or both of the gRNAs configured such that it comprises properties: a and c.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a, b, and c.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(i), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(i), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(i), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(i), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(i), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ii), and c(i). In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ii), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ii), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ii), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ii), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iii), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iii), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iii), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iii), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iii), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iv), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iv), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iv), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iv), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(iv), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(v), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(v), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(v), c, and d. In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(v), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(v), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vi), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vi), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vi), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vi), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vi), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vii), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vii), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vii), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vii), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(vii), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(viii), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(viii), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(viii), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(viii), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(viii), c, d, and e. In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ix), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ix), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ix), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ix), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(ix), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(x), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(x), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(x), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(x), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(x), c, d, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(xi), and c(i).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(xi), and c(ii).

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(xi), c, and d.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(xi), c, and e.

In an embodiment, one or both of the gRNAs is configured such that it comprises properties: a(i), b(xi), c, d, and e.

In an embodiment, the gRNAs are used with a Cas9 nickase molecule having HNH activity, e.g., a Cas9 molecule having the RuvC activity inactivated, e.g., a Cas9 molecule having a mutation at D10, e.g., the D10A mutation. In an embodiment, the gRNAs are used with a Cas9 nickase molecule having RuvC activity, e.g., a Cas9 molecule having the HNH activity inactivated, e.g., a Cas9 molecule having a mutation at H840, e.g., the H840A mutation.

In an embodiment, the gRNAs are used with a Cas9 nickase molecule having RuvC activity, e.g., a Cas9 molecule having the HNH activity inactivated, e.g., a Cas9 molecule having a mutation at N863, e.g., the N863A mutation.

VI. Target Cells

Cas9 molecules and gRNA molecules, e.g., a Cas9 molecule/gRNA molecule complex, can be used to manipulate a cell, e.g., to edit a target nucleic acid, in a wide variety of cells.

In an embodiment, a cell is manipulated by editing (e.g., introducing a mutation in) one or more target genes, e.g., HSV-2 target genes, e.g., target genes as described herein, e.g., one or more of the UL19, UL30, UL48 or UL54 genes. In an embodiment, the expression of one or more target genes (e.g., one or more target genes described herein, e.g., one or more of the UL19, UL30, UL48 or UL54 genes) is modulated, e.g., in vivo.

The Cas9 and gRNA molecules described herein can be delivered to a target cell. In an embodiment, the target cell is an epithelial cell, e.g., an epithelial cell of the oropharynx (including, e.g., an epithelial cell of the nose, gums, lips, tongue or pharynx), an epithelial cell of the finger or fingernail bed, or an epithelial cell of the ano-genital area (including, e.g., an epithelial cell of the penis, scrotum, vulva, vagina, cervix, anus or thighs). In an embodiment, the target cell is a neuronal cell, e.g., a cranial ganglion neuron (e.g. a trigeminal ganglion neuron, e.g., an oculomotor nerve ganglion neuron, e.g., an abducens nerve ganglion neuron, e.g., a trochlear nerve ganglion neuron), e.g. a cervical ganglion neuron, e.g., a sacral ganglion neuron, a sensory ganglion neuron, a cortical neuron, a cerebellar neuron or a hippocampal neuron. In an embodiment, the target cell is an optic cell, e.g. an epithelial cell of the eye, e.g. an epithelial cell of the eyelid, e.g., a conjunctival cell, e.g., a conjunctival epithelial cell, e.g., a corneal keratocyte, e.g., a limbus cell, e.g., a corneal epithelial cell, e.g., a corneal stromal cell, e.g., a ciliary body cell, e.g., a scleral cell, e.g., a lens cell, e.g., a choroidal cell, e.g., a retinal cell, e.g., a rod photoreceptor cell, e.g., a cone photoreceptor cell, e.g., a retinal pigment epithelium cell, e.g., a horizontal cell, e.g., an amacrine cell, e.g., a ganglion cell. VII. Delivery, Formulations and Routes of Administration

The components, e.g., a Cas9 molecule and gRNA molecule can be delivered or formulated in a variety of forms, see, e.g., Tables 23-24. In an embodiment, one Cas9 molecule and two or more (e.g., 2, 3, 4, or more) different gRNA molecules are delivered, e.g., by an AAV vector. In an embodiment, the sequence encoding the Cas9 molecule and the sequence(s) encoding the two or more (e.g., 2, 3, 4, or more) different gRNA molecules are present on the same nucleic acid molecule, e.g., an AAV vector. When a Cas9 or gRNA component is encoded as DNA for delivery, the DNA will typically but not necessarily include a control region, e.g., comprising a promoter, to effect expression. Useful promoters for Cas9 molecule sequences include CMV, EFS, EF-la, MSCV, PGK, CAG control promoters. In an embodiment, the promoter is a constitutive promoter. In another embodiment, the promoter is a tissue specific promoter. Useful promoters for gRNAs include HI, 7SK, tRNA, and U6 promoters. Promoters with similar or dissimilar strengths can be selected to tune the expression of components. Sequences encoding a Cas9 molecule can comprise a nuclear localization signal (NLS), e.g., an SV40 NLS. In an embodiment, the sequence encoding a Cas9 molecule comprises at least two nuclear localization signals. In an embodiment a promoter for a Cas9 molecule or a gRNA molecule can be, independently, inducible, tissue specific, or cell specific.

Table 23 provides examples of how the components can be formulated, delivered, or administered.

Table 23

in vitro transcribed or synthesized RNA. mRNA DNA In this embodiment, a Cas9 molecule, typically

an eaCas9 molecule, is translated from in vitro transcribed mRNA, and a gRNA is transcribed from DNA.

Protein DNA In this embodiment, a Cas9 molecule, typically

an eaCas9 molecule, is provided as a protein,

and a gRNA is transcribed from DNA.

Protein RNA In this embodiment, an eaCas9 molecule is

provided as a protein, and a gRNA is provided

as transcribed or synthesized RNA.

Table 24 summarizes various delivery methods for the components of a Cas system, e.g., the Cas9 molecule component and the gRNA molecule component, as described herein. Table 24

Mammalian YES Transient NO Nucleic Acids

Virus-like

Particles

Biological YES Transient NO Nucleic Acids liposomes:

Erythrocyte

Ghosts and

Exosomes

DNA-based Delivery of a Cas9 molecule and/or one or more gRNA molecule

Nucleic acids encoding Cas9 molecules (e.g., eaCas9 molecules) and/or gRNA molecules, can be administered to subjects or delivered into cells by art-known methods or as described herein. For example, Cas9-encoding and/or gRNA-encoding DNA can be delivered, e.g., by vectors (e.g., viral or non-viral vectors), non- vector based methods (e.g., using naked DNA or DNA complexes), or a combination thereof.

DNA encoding Cas9 molecules (e.g., eaCas9 molecules) and/or gRNA molecules can be conjugated to molecules promoting uptake by the target cells (e.g., the target cells described herein).

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a vector (e.g., viral vector/virus or plasmid).

A vector can comprise a sequence that encodes a Cas9 molecule and/or a gRNA molecule. A vector can also comprise a sequence encoding a signal peptide (e.g., for nuclear localization, nucleolar localization, mitochondrial localization), fused, e.g., to a Cas9 molecule sequence. For example, ae vector can comprise a nuclear localization sequence (e.g., from SV40) fused to the sequence encoding the Cas9 molecule.

One or more regulatory/control elements, e.g., a promoter, an enhancer, an intron, a polyadenylation signal, a Kozak consensus sequence, internal ribosome entry sites (IRES), a 2A sequence, and splice acceptor or donor can be included in the vectors. In some embodiments, the promoter is recognized by RNA polymerase II (e.g., a CMV promoter). In other embodiments, the promoter is recognized by RNA polymerase III (e.g., a U6 promoter). In some embodiments, the promoter is a regulated promoter (e.g., inducible promoter). In other embodiments, the promoter is a constitutive promoter. In some embodiments, the promoter is a tissue specific promoter. In some embodiments, the promoter is a viral promoter. In other embodiments, the promoter is a non- viral promoter.

In some embodiments, the vector or delivery vehicle is a viral vector (e.g., for generation of recombinant viruses). In some embodiments, the virus is a DNA virus (e.g., dsDNA or ssDNA virus). In other embodiments, the virus is an RNA virus (e.g., an ssRNA virus). Exemplary viral vectors/viruses include, e.g., retroviruses, lentiviruses, adenovirus, adeno-associated virus (AAV), vaccinia viruses, poxviruses, and herpes simplex viruses.

In some embodiments, the virus infects dividing cells. In other embodiments, the virus infects non-dividing cells. In some embodiments, the virus infects both dividing and non-dividing cells. In some embodiments, the virus can integrate into the host genome. In some embodiments, the virus is engineered to have reduced immunity, e.g., in human. In some embodiments, the virus is replication-competent. In other embodiments, the virus is replication-defective, e.g., having one or more coding regions for the genes necessary for additional rounds of virion replication and/or packaging replaced with other genes or deleted. In some embodiments, the virus causes transient expression of the Cas9 molecule and/or the gRNA molecule. In other embodiments, the virus causes long-lasting, e.g., at least 1 week, 2 weeks, 1 month, 2 months, 3 months, 6 months, 9 months, 1 year, 2 years, or permanent expression, of the Cas9 molecule and/or the gRNA molecule. The packaging capacity of the viruses may vary, e.g., from at least about 4 kb to at least about 30 kb, e.g., at least about 5 kb, 10 kb, 15 kb, 20 kb, 25 kb, 30 kb, 35 kb, 40 kb, 45 kb, or 50 kb.

In an embodiment, the viral vector recognizes a specific cell type or tissue. For example, the viral vector can be pseudotyped with a different/alternative viral envelope glycoprotein; engineered with a cell type-specific receptor (e.g., genetic modification(s) of one or more viral envelope glycoproteins to incorporate a targeting ligand such as a peptide ligand, a single chain antibody, or a growth factor); and/or engineered to have a molecular bridge with dual specificities with one end recognizing a viral glycoprotein and the other end recognizing a moiety of the target cell surface (e.g., a ligand-receptor, monoclonal antibody, avidin-biotin and chemical conjugation).

Exemplary viral vectors/viruses include, e.g., retroviruses, lentiviruses, adenovirus, adeno-associated virus (AAV), vaccinia viruses, poxviruses, and herpes simplex viruses.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a recombinant retrovirus. In some embodiments, the retrovirus (e.g., Moloney murine leukemia virus) comprises a reverse transcriptase, e.g., that allows integration into the host genome. In some embodiments, the retrovirus is replication-competent. In other embodiments, the retrovirus is replication-defective, e.g., having one of more coding regions for the genes necessary for additional rounds of virion replication and packaging replaced with other genes, or deleted. In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a recombinant lentivirus. For example, the lentivirus is replication-defective, e.g., does not comprise one or more genes required for viral replication.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a recombinant adenovirus. In some embodiments, the adenovirus is engineered to have reduced immunity in human.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a recombinant AAV. In some embodiments, the AAV does not incorporate its genome into that of a host cell, e.g., a target cell as describe herein. In some embodiments, the AAV can incorporate at least part of its genome into that of a host cell, e.g., a target cell as described herein. In some embodiments, the AAV is a self-complementary adeno-associated virus (scAAV), e.g., a scAAV that packages both strands which anneal together to form double stranded DNA. AAV serotypes that may be used in the disclosed methods, include AAV1, AAV2, modified AAV2 (e.g., modifications at Y444F, Y500F, Y730F and/or S662V), AAV3, modified AAV3 (e.g., modifications at Y705F, Y731F and/or T492V), AAV4,

AAV5, AAV6, modified AAV6 (e.g., modifications at S663V and/or T492V), AAV8, AAV 8.2, AAV9, AAV rh 10, and pseudotyped AAV, such as AAV2/8, AAV2/5 and AAV2/6 can also be used in the disclosed methods. In an embodiment, an AAV capsid that can be used in the methods described herein is a capsid sequence from serotype AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV.rh8, AAV.rhlO, AAV.rh32/33, AAV.rh43, AAV.rh64Rl, or AAV7m8.

In an embodiment, the Cas9- and/or gRNA-encoding DNA is delivered in a re- engineered AAV capsid, e.g., with 50% or greater, e.g., 60% or greater, 70% or greater, 80% or greater, 90% or greater, or 95% or greater, sequence homology with a capsid sequence from serotypes AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV.rh8, AAV.rhlO, AAV.rh32/33, AAV.rh43, or AAV.rh64Rl.

In an embodiment, the Cas9- and/or gRNA-encoding DNA is delivered by a chimeric AAV capsid. Exemplary chimeric AAV capsids include, but are not limited to, AAV9il, AAV2i8, AAV-DJ, AAV2G9, AAV2i8G9, or AAV8G9.

In an embodiment, the AAV is a self-complementary adeno-associated virus

(scAAV), e.g., a scAAV that packages both strands which anneal together to form double stranded DNA.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a hybrid virus, e.g., a hybrid of one or more of the viruses described herein. In an embodiment, the hybrid virus is hybrid of an AAV (e.g., of any AAV serotype), with a Bocavirus, B 19 virus, porcine AAV, goose AAV, feline AAV, canine AAV, or MVM.

A Packaging cell is used to form a virus particle that is capable of infecting a target cell. Such a cell includes a 293 cell, which can package adenovirus, and a ψ2 cell or a PA317 cell, which can package retrovirus. A viral vector used in gene therapy is usually generated by a producer cell line that packages a nucleic acid vector into a viral particle. The vector typically contains the minimal viral sequences required for packaging and subsequent integration into a host or target cell (if applicable), with other viral sequences being replaced by an expression cassette encoding the protein to be expressed, eg. Cas9. For example, an AAV vector used in gene therapy typically only possesses inverted terminal repeat (ITR) sequences from the AAV genome which are required for packaging and gene expression in the host or target cell. The missing viral functions can be supplied in trans by the packaging cell line and/or plasmid containing E2A, E4, and VA genes from adenovirus, and plasmid encoding Rep and Cap genes from AAV, as described in "Triple Transfection Protocol." Henceforth, the viral DNA is packaged in a cell line, which contains a helper plasmid encoding the other AAV genes, namely rep and cap, but lacking ITR sequences. In embodiment, the viral DNA is packaged in a producer cell line, which contains E1A and/or E1B genes from adenovirus. The cell line is also infected with adenovirus as a helper. The helper virus (e.g., adenovirus or HSV) or helper plasmid promotes replication of the AAV vector and expression of AAV genes from the helper plasmid with ITRs. The helper plasmid is not packaged in significant amounts due to a lack of ITR sequences. Contamination with adenovirus can be reduced by, e.g., heat treatment to which adenovirus is more sensitive than AAV.

In an embodiment, the viral vector has the ability of cell type and/or tissue type recognition. For example, the viral vector can be pseudotyped with a different/alternative viral envelope glycoprotein; engineered with a cell type-specific receptor (e.g.,

geneticmodification of the viral envelope glycoproteins to incorporate targeting ligands such as a peptide ligand, a single chain antibodie, a growth factor); and/or engineered to have a molecular bridge with dual specificities with one end recognizing a viral glycoprotein and the other end recognizing a moiety of the target cell surface (e.g., ligand-receptor, monoclonal antibody, avidin-biotin and chemical conjugation).

In an embodiment, the viral vector achieves cell type specific expression. For example, a tissue-specific promoter can be constructed to restrict expression of the transgene (Cas 9 and gRNA) in only the target cell. The specificity of the vector can also be mediated by microRNA-dependent control of transgene expression. In an embodiment, the viral vector has increased efficiency of fusion of the viral vector and a target cell membrane. For example, a fusion protein such as fusion-competent hemagglutin (HA) can be incorporated to increase viral uptake into cells. In an embodiment, the viral vector has the ability of nuclear localization. For example, aviruse that requires the breakdown of the nuclear envelope (during cell division) and therefore will not infect a non-diving cell can be altered to incorporate a nuclear localization peptide in the matrix protein of the virus thereby enabling the transduction of non-proliferating cells.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a non- vector based method (e.g., using naked DNA or DNA complexes). For example, the DNA can be delivered, e.g., by organically modified silica or silicate (Ormosil), electroporation, gene gun, sonoporation, magnetofection, lipid- mediated transfection, dendrimers, inorganic nanoparticles, calcium phosphates, or a combination thereof.

In some embodiments, the Cas9- and/or gRNA-encoding DNA is delivered by a combination of a vector and a non- vector based method. For example, a virosome comprises a liposome combined with an inactivated virus (e.g., HIV or influenza virus), which can result in more efficient gene transfer, e.g., in a respiratory epithelial cell than either a viral or a liposomal method alone.

In an embodiment, the delivery vehicle is a non- viral vector. In an embodiment, the non- viral vector is an inorganic nanoparticle. Exemplary inorganic nanoparticles include, e.g., magnetic nanoparticles (e.g., Fe₃Mn0₂) or silica. The outer surface of the nanoparticle can be conjugated with a positively charged polymer (e.g., polyethylenimine, polylysine, polyserine) which allows for attachment (e.g., conjugation or entrapment) of payload.. In an embodiment, the non- viral vector is an organic nanoparticle (e.g., entrapment of the payload inside the nanoparticle). Exemplary organic nanoparticles include, e.g., SNALP liposomes that contain cationic lipids together with neutral helper lipids which are coated with polyethylene glycol (PEG) and protamine and nucleic acid complex coated with lipid coating.

Exemplary lipids for gene transfer are shown below in Table 25.

Table 25: Lipids Used for Gene Transfer

Lipid Abbreviation Feature l,2-Dioleoyl-sn-glycero-3-phosphatidylcholine DOPC Helper l,2-Dioleoyl-sn-glycero-3-phosphatidylethanolamine DOPE Helper

Cholesterol Helper

A-[l-(2,3-Dioleyloxy)prophyl]AWA -trimethylammonium chloride DOTMA Cationic l,2-Dioleoyloxy-3-trimethylammonium-propane DOTAP Cationic

Dioctadecylamidoglycylspermine DOGS Cationic

noey- met y- - met yamno utyrate n- - atonc

Exemplary polymers for gene transfer are shown below in Table 26.

Table 26: Polymers Used for Gene Transfer

extran-sperm ne -

In an embodiment, the vehicle has targeting modifications to increase target cell update of nanoparticles and liposomes, e.g., cell specific antigens, monoclonal antibodies, single chain antibodies, aptamers, polymers, sugars, and cell penetrating peptides. In an embodiment, the vehicle uses fusogenic and endosome-destabilizing peptides/polymers. In an embodiment, the vehicle undergoes acid-triggered conformational changes (e.g., to accelerate endosomal escape of the cargo). In an embodiment, a stimuli-cleavable polymer is used, e.g., for release in a cellular compartment. For example, disulfide-based cationic polymers that are cleaved in the reducing cellular environment can be used.

In an embodiment, the delivery vehicle is a biological non- viral delivery vehicle. In an embodiment, the vehicle is an attenuated bacterium (e.g., naturally or artificially engineered to be invasive but attenuated to prevent pathogenesis and expressing the transgene (e.g., Listeria monocytogenes, certain Salmonella strains, Bifidobacterium longum, and modified Escherichia coli), bacteria having nutritional and tissue-specific tropism to target specific tissues, bacteria having modified surface proteins to alter target tissue specificity). In an embodiment, the vehicle is a genetically modified bacteriophage (e.g., engineered phages having large packaging capacity, less immunogenic, containing mammalian plasmid maintenance sequences and having incorporated targeting ligands). In an embodiment, the vehicle is a mammalian virus-like particle. For example, modified viral particles can be generated (e.g., by purification of the "empty" particles followed by ex vivo assembly of the virus with the desired cargo). The vehicle can also be engineered to incorporate targeting ligands to alter target tissue specificity. In an embodiment, the vehicle is a biological liposome. For example, the biological liposome is a phospholipid-based particle derived from human cells (e.g., erythrocyte ghosts, which are red blood cells broken down into spherical structures derived from the subject (e.g., tissue targeting can be achieved by attachment of various tissue or cell-specific ligands), or secretory exosomes -subject (i.e., patient) derived membrane-bound nanovescicle (30 -100 nm) of endocytic origin (e.g., can be produced from various cell types and can therefore be taken up by cells without the need of for targeting ligands).

In an embodiment, one or more nucleic acid molecules (e.g., DNA molecules) other than the components of a Cas system, e.g., the Cas9 molecule component and/or the gRNA molecule component described herein, are delivered. In an embodiment, the nucleic acid molecule is delivered at the same time as one or more of the components of the Cas system are delivered. In an embodiment, the nucleic acid molecule is delivered before or after (e.g., less than about 30 minutes, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours, 12 hours, 1 day, 2 days, 3 days, 1 week, 2 weeks, or 4 weeks) one or more of the components of the Cas system are delivered. In an embodiment, the nucleic acid molecule is delivered by a different means than one or more of the components of the Cas system, e.g., the Cas9 molecule component and/or the gRNA molecule component, are delivered. The nucleic acid molecule can be delivered by any of the delivery methods described herein. For example, the nucleic acid molecule can be delivered by a viral vector, e.g., an integration-deficient lentivirus, and the Cas9 molecule component and/or the gRNA molecule component can be delivered by electroporation, e.g., such that the toxicity caused by nucleic acids (e.g., DNAs) can be reduced. In an embodiment, the nucleic acid molecule encodes a therapeutic protein, e.g., a protein described herein. In an embodiment, the nucleic acid molecule encodes an RNA molecule, e.g., an RNA molecule described herein.

Delivery of RNA encoding a Cas 9 molecule

RNA encoding Cas9 molecules (e.g., eaCas9 molecules or eiCas9 molecules) and/or gRNA molecules, can be delivered into cells, e.g., target cells described herein, by art-known methods or as described herein. For example, Cas9-encoding and/or gRNA-encoding RNA can be delivered, e.g., by microinjection, electroporation, lipid-mediated transfection, peptide-mediated delivery, or a combination thereof. Cas9-encoding and/or gRNA-encoding RNA can be conjugated to molecules promoting uptake by the target cells (e.g., target cells described herein). Delivery Cas9 molecule protein

Cas9 molecules (e.g., eaCas9 molecules or eiCas9 molecules) can be delivered into cells by art-known methods or as described herein. For example, Cas9 protein molecules can be delivered, e.g., by microinjection, electroporation, lipid-mediated transfection, peptide- mediated delivery, or a combination thereof. Delivery can be accompanied by DNA encoding a gRNA or by a gRNA. Cas9 protein can be conjugated to molecules promoting uptake by the target cells (e.g., target cells described herein).

Multiplex Delivery of gRNAs

In some embodiments, the Cas9 molecule, e.g., double-stranded nuclease or single- stranded nickase, is delivered together with gRNAs or gRNA pairs targeting more than one HSV-2 target position. The gRNAs can be provided in different forms, e.g., the forms described in Table 23. The gRNAs can be delivered by different methods, e.g., the methods described in Table 24. It is contemplated herein that the Cas9 molecule, and gRNAs or gRNA pairs targeting more than one HSV-2 target position could be contained within a single or multiple delivery vector or formulation including, e.g., those described above.

Route of Administration

Systemic modes of administration include oral and parenteral routes. Parenteral routes include, by way of example, intravenous, intrarterial, intraosseous, intramuscular, intradermal, subcutaneous, intranasal and intraperitoneal routes. Components administered systemically may be modified or formulated to target the components to epithelial or neuronal cells.

Local modes of administration include, by way of example, topical, intrathecal, intraspinal, intra-cerebroventricular, intraparenchymal (e.g., into the parenchyma of the brain or spinal cord), and intraocular, including intra vitreal, intracorneal, and intraretinal routes. In an embodiment, local modes of administration include intraocular, e.g., intrascleral, e.g., intracorneal, e.g., intravitreal, e.g., intraretinal, e.g. submacular, e.g., intra-lens. In an embodiment, components described herein are delivered subretinally, e.g., by subretinal injection. In an embodiment, components described herein are delivered by intravitreal injection.

In an embodiment, local modes of administration include topical, e.g., topical optic delivery, e.g., topical dermal delivery. In an embodiment, local modes of administration include intra-parenchymal into the dorsal root ganglion at the level of the trigeminal nerve. In an embodiment, local modes of administration include intra-parenchymal into the dorsal root ganglion at the level of the sacral ganglia. In an embodiment, local modes of administration include intra-parenchymal into the dorsal root ganglion at the level of the lumbar ganglia. In an embodiment, local modes of administration include intra-parenchymal into the dorsal root ganglion at the level of the thoracic ganglia. In an embodiment, local modes of administration include intra- parenchymal into the dorsal root ganglion at the level of the cervical ganglia, e.g., superior cervical ganglion, e.g., middle cervical ganglion, e.g., inferior cervical ganglion.

In an embodiment, significantly smaller amounts of the components (compared with systemic approaches) may exert an effect when administered locally (for example, topically; for example intracorneally) compared to when administered systemically (for example, intravenously). Local modes of administration can reduce or eliminate the incidence of potentially toxic side effects that may occur when therapeutically effective amounts of a component are administered systemically.

In an embodiment, components described herein are delivered by topical

administration to epithelial cells, e.g., epithelial cells of the oropharynx (including, e.g., epithelial cells of the nose, gums, lips, tongue or pharynx), epithelial cells of the finger or fingernail bed, or epithelial cells of the ano-genital area (including, e.g., epithelial cells of the penis, vulva, vagina or anus). In an embodiment, components described herein are delivered by topical administration to the eye (including, e.g., corneal epithelium, e.g., corneal stroma, e.g., epithelium of upper and lower eyelid, e.g., lens).

In an embodiment, nanoparticle or viral, e.g., AAV vector, delivery is via topical administration.

Administration may be provided as a periodic bolus or as continuous infusion from an internal reservoir or from an external reservoir (for example, from an intravenous bag). Components may be administered locally, for example, by continuous release from a sustained release drug delivery device.

Administration may be provided as continuous infusion from an internal reservoir (for example, from an implant disposed at an intra- or extra-ocular location (see, U.S. Pat. Nos. 5,443,505 and 5,766,242)) or from an external reservoir (for example, from an intravenous bag). Components may be administered locally, for example, by continuous release from a sustained release drug delivery device immobilized to an inner wall of the eye or via targeted transscleral controlled release into the choroid (see, for example, PCT/USOO/00207, PCT/US02/14279, Ambati et al. (2000) Invest. Ophthalmol. Vis. Sci. 41: 1181-1185, and Ambati et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:1186-1191). A variety of devices suitable for administering components locally to the inside of the eye are known in the art. See, for example, U.S. Pat. Nos. 6,251,090, 6,299,895, 6,416,777, 6,413,540, and

PCT/USOO/28187.

In addition, components may be formulated to permit release over a prolonged period of time. A release system can include a matrix of a biodegradable material or a material which releases the incorporated components by diffusion. The components can be homogeneously or heterogeneously distributed within the release system. A variety of release systems may be useful, however, the choice of the appropriate system will depend upon rate of release required by a particular application. Both non-degradable and degradable release systems can be used. Suitable release systems include polymers and polymeric matrices, non-polymeric matrices, or inorganic and organic excipients and diluents such as, but not limited to, calcium carbonate and sugar (for example, trehalose). Release systems may be natural or synthetic. However, synthetic release systems are preferred because generally they are more reliable, more reproducible and produce more defined release profiles. The release system material can be selected so that components having different molecular weights are released by diffusion through or degradation of the material.

Representative synthetic, biodegradable polymers include, for example: polyamides such as poly(amino acids) and poly(peptides); polyesters such as poly(lactic acid), poly(glycolic acid), poly(lactic-co-glycolic acid), and poly(caprolactone); poly(anhydrides); polyorthoesters; polycarbonates; and chemical derivatives thereof (substitutions, additions of chemical groups, for example, alkyl, alkylene, hydroxylations, oxidations, and other modifications routinely made by those skilled in the art), copolymers and mixtures thereof. Representative synthetic, non-degradable polymers include, for example: polyethers such as poly(ethylene oxide), poly(ethylene glycol), and poly(tetramethylene oxide); vinyl polymers- polyacrylates and polymethacrylates such as methyl, ethyl, other alkyl, hydroxyethyl methacrylate, acrylic and methacrylic acids, and others such as poly(vinyl alcohol), poly(vinyl pyrolidone), and poly(vinyl acetate); poly(urethanes); cellulose and its derivatives such as alkyl, hydroxyalkyl, ethers, esters, nitrocellulose, and various cellulose acetates; polysiloxanes; and any chemical derivatives thereof (substitutions, additions of chemical groups, for example, alkyl, alkylene, hydroxylations, oxidations, and other modifications routinely made by those skilled in the art), copolymers and mixtures thereof.

Poly(lactide-co-glycolide) microsphere can also be used for injection. Typically the microspheres are composed of a polymer of lactic acid and glycolic acid, which are structured to form hollow spheres. The spheres can be approximately 15-30 microns in diameter and can be loaded with components described herein. Bi-Modal or Differential Delivery of Components

Separate delivery of the components of a Cas system, e.g., the Cas9 molecule component and the gRNA molecule component, and more particularly, delivery of the components by differing modes, can enhance performance, e.g., by improving tissue specificity and safety.

In an embodiment, the Cas 9 molecule and the gRNA molecule are delivered by different modes, or as sometimes referred to herein as differential modes. Different or differential modes, as used herein, refer modes of delivery that confer different

pharmacodynamic or pharmacokinetic properties on the subject component molecule, e.g., a Cas9 molecule, or gRNA molecule,. For example, the modes of delivery can result in different tissue distribution, different half-life, or different temporal distribution, e.g., in a selected compartment, tissue, or organ.

Some modes of delivery, e.g., delivery by a nucleic acid vector that persists in a cell, or in progeny of a cell, e.g., by autonomous replication or insertion into cellular nucleic acid, result in more persistent expression of and presence of a component. Examples include viral, e.g., adeno- associated virus or lentivirus, delivery.

By way of example, the components, e.g., a Cas9 molecule and a gRNA molecule, can be delivered by modes that differ in terms of resulting half-life or persistent of the delivered component the body, or in a particular compartment, tissue or organ. In an embodiment, a gRNA molecule can be delivered by such modes. The Cas9 molecule component can be delivered by a mode which results in less persistence or less exposure to the body or a particular compartment or tissue or organ.

More generally, in an embodiment, a first mode of delivery is used to deliver a first component and a second mode of delivery is used to deliver a second component. The first mode of delivery confers a first pharmacodynamic or pharmacokinetic property. The first pharmacodynamic property can be, e.g., distribution, persistence, or exposure, of the component, or of a nucleic acid that encodes the component, in the body, a compartment, tissue or organ. The second mode of delivery confers a second pharmacodynamic or pharmacokinetic property. The second pharmacodynamic property can be, e.g., distribution, persistence, or exposure, of the component, or of a nucleic acid that encodes the component, in the body, a compartment, tissue or organ.

In an embodiment, the first pharmacodynamic or pharmacokinetic property, e.g., distribution, persistence or exposure, is more limited than the second pharmacodynamic or pharmacokinetic property.

In an embodiment, the first mode of delivery is selected to optimize, e.g., minimize, a pharmacodynamic or pharmacokinetic property, e.g., distribution, persistence or exposure.

In an embodiment, the second mode of delivery is selected to optimize, e.g., maximize, a pharmacodynamic or pharmcokinetic property, e.g., distribution, persistence or exposure.

In an embodiment, the first mode of delivery comprises the use of a relatively persistent element, e.g., a nucleic acid, e.g., a plasmid or viral vector, e.g., an AAV or lentivirus. As such vectors are relatively persistent product transcribed from them would be relatively persistent.

In an embodiment, the second mode of delivery comprises a relatively transient element, e.g., an RNA or protein.

In an embodiment, the first component comprises gRNA, and the delivery mode is relatively persistent, e.g., the gRNA is transcribed from a plasmid or viral vector, e.g., an

AAV or lentivirus. Transcription of these genes would be of little physiological consequence because the genes do not encode for a protein product, and the gRNAs are incapable of acting in isolation. The second component, a Cas9 molecule, is delivered in a transient manner, for example as mRNA or as protein, ensuring that the full Cas9 molecule/gRNA molecule complex is only present and active for a short period of time.

Furthermore, the components can be delivered in different molecular form or with different delivery vectors that complement one another to enhance safety and tissue specificity.

Use of differential delivery modes can enhance performance, safety and efficacy. E.g., the likelihood of an eventual off-target modification can be reduced. Delivery of immunogenic components, e.g., Cas9 molecules, by less persistent modes can reduce immunogenicity, as peptides from the bacterially-derived Cas enzyme are displayed on the surface of the cell by MHC molecules. A two-part delivery system can alleviate these drawbacks.

Differential delivery modes can be used to deliver components to different, but overlapping target regions. The formation active complex is minimized outside the overlap of the target regions. Thus, in an embodiment, a first component, e.g., a gRNA molecule is delivered by a first delivery mode that results in a first spatial, e.g., tissue, distribution. A second component, e.g., a Cas9 molecule is delivered by a second delivery mode that results in a second spatial, e.g., tissue, distribution. In an embodiment, the first mode comprises a first element selected from a liposome, nanoparticle, e.g., polymeric nanoparticle, and a nucleic acid, e.g., viral vector. The second mode comprises a second element selected from the group. In an embodiment, the first mode of delivery comprises a first targeting element, e.g., a cell specific receptor or an antibody, and the second mode of delivery does not include that element. In embodiment, the second mode of delivery comprises a second targeting element, e.g., a second cell specific receptor or second antibody.

When the Cas9 molecule is delivered in a virus delivery vector, a liposome, or polymeric nanoparticle, there is the potential for delivery to and therapeutic activity in multiple tissues, when it may be desirable to only target a single tissue. A two-part delivery system can resolve this challenge and enhance tissue specificity. If the gRNA molecule and the Cas9 molecule are packaged in separated delivery vehicles with distinct but overlapping tissue tropism, the fully functional complex is only be formed in the tissue that is targeted by both vectors.

Ex vivo delivery

In some embodiments, components described in Table 23 are introduced into cells which are then introduced into the subject, e.g., cells are removed from a subject, manipulated ex vivo and then introduced into the subject. Methods of introducing the components can include, e.g., any of the delivery methods described in Table 24. VIII. Modified Nucleosides, Nucleotides, and Nucleic Acids

Modified nucleosides and modified nucleotides can be present in nucleic acids, e.g., particularly gRNA, but also other forms of RNA, e.g., mRNA, RNAi, or siRNA. As described herein, "nucleoside" is defined as a compound containing a five-carbon sugar molecule (a pentose or ribose) or derivative thereof, and an organic base, purine or pyrimidine, or a derivative thereof. As described herein, "nucleotide" is defined as a nucleoside further comprising a phosphate group.

Modified nucleosides and nucleotides can include one or more of: (i) alteration, e.g., replacement, of one or both of the non-linking phosphate oxygens and/or of one or more of the linking phosphate oxygens in the phosphodiester backbone linkage;

(ii) alteration, e.g., replacement, of a constituent of the ribose sugar, e.g., of the 2' hydroxyl on the ribose sugar;

(iii) wholesale replacement of the phosphate moiety with "dephospho" linkers;

(iv) modification or replacement of a naturally occurring nucleobase;

(v) replacement or modification of the ribose-phosphate backbone;

(vi) modification of the 3' end or 5' end of the oligonucleotide, e.g., removal, modification or replacement of a terminal phosphate group or conjugation of a moiety; and

(vii) modification of the sugar.

The modifications listed above can be combined to provide modified nucleosides and nucleotides that can have two, three, four, or more modifications. For example, a modified nucleoside or nucleotide can have a modified sugar and a modified nucleobase. In an embodiment, every base of a gRNA is modified, e.g., all bases have a modified phosphate group, e.g., all are phosphorothioate groups. In an embodiment, all, or substantially all, of the phosphate groups of a unimolecular or modular gRNA molecule are replaced with phosphorothioate groups.

In an embodiment, modified nucleotides, e.g., nucleotides having modifications as described herein, can be incorporated into a nucleic acid, e.g., a "modified nucleic acid." In some embodiments, the modified nucleic acids comprise one, two, three or more modified nucleotides. In some embodiments, at least 5% (e.g., at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or about 100%) of the positions in a modified nucleic acid are a modified nucleotides.

Unmodified nucleic acids can be prone to degradation by, e.g., cellular nucleases. For example, nucleases can hydrolyze nucleic acid phosphodiester bonds. Accordingly, in one aspect the modified nucleic acids described herein can contain one or more modified nucleosides or nucleotides, e.g., to introduce stability toward nucleases.

In some embodiments, the modified nucleosides, modified nucleotides, and modified nucleic acids described herein can exhibit a reduced innate immune response when introduced into a population of cells, both in vivo and ex vivo. The term "innate immune response" includes a cellular response to exogenous nucleic acids, including single stranded nucleic acids, generally of viral or bacterial origin, which involves the induction of cytokine expression and release, particularly the interferons, and cell death. In some embodiments, the modified nucleosides, modified nucleotides, and modified nucleic acids described herein can disrupt binding of a major groove interacting partner with the nucleic acid. In some embodiments, the modified nucleosides, modified nucleotides, and modified nucleic acids described herein can exhibit a reduced innate immune response when introduced into a population of cells, both in vivo and ex vivo, and also disrupt binding of a major groove interacting partner with the nucleic acid.

Definitions of Chemical Groups

As used herein, "alkyl" is meant to refer to a saturated hydrocarbon group which is straight-chained or branched. Example alkyl groups include methyl (Me), ethyl (Et), propyl (e.g. , n-propyl and isopropyl), butyl (e.g. , n-butyl, isobutyl, t-butyl), pentyl (e.g. , n-pentyl, isopentyl, neopentyl), and the like. An alkyl group can contain from 1 to about 20, from 2 to about 20, from 1 to about 12, from 1 to about 8, from 1 to about 6, from 1 to about 4, or from 1 to about 3 carbon atoms.

As used herein, "aryl" refers to monocyclic or polycyclic (e.g. , having 2, 3 or 4 fused rings) aromatic hydrocarbons such as, for example, phenyl, naphthyl, anthracenyl, phenanthrenyl, indanyl, indenyl, and the like. In some embodiments, aryl groups have from 6 to about 20 carbon atoms.

As used herein, "alkenyl" refers to an aliphatic group containing at least one double bond.

As used herein, "alkynyl" refers to a straight or branched hydrocarbon chain containing 2-12 carbon atoms and characterized in having one or more triple bonds.

Examples of alkynyl groups include, but are not limited to, ethynyl, propargyl, and 3- hexynyl.

As used herein, "arylalkyl" or "aralkyl" refers to an alkyl moiety in which an alkyl hydrogen atom is replaced by an aryl group. Aralkyl includes groups in which more than one hydrogen atom has been replaced by an aryl group. Examples of "arylalkyl" or "aralkyl" include benzyl, 2-phenylethyl, 3-phenylpropyl, 9-fluorenyl, benzhydryl, and trityl groups.

As used herein, "cycloalkyl" refers to a cyclic, bicyclic, tricyclic, or polycyclic non- aromatic hydrocarbon groups having 3 to 12 carbons. Examples of cycloalkyl moieties include, but are not limited to, cyclopropyl, cyclopentyl, and cyclohexyl. As used herein, "heterocyclyl" refers to a monovalent radical of a heterocyclic ring system. Representative heterocyclyls include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, pyrrolidonyl, piperidinyl, pyrrolinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, and morpholinyl.

As used herein, "heteroaryl" refers to a monovalent radical of a heteroaromatic ring system. Examples of heteroaryl moieties include, but are not limited to, imidazolyl, oxazolyl, thiazolyl, triazolyl, pyrrolyl, furanyl, indolyl, thiophenyl pyrazolyl, pyridinyl, pyrazinyl, pyridazinyl, pyrimidinyl, indolizinyl, purinyl, naphthyridinyl, quinolyl, and pteridinyl. Phosphate Backbone Modifications

The Phosphate Group

In some embodiments, the phosphate group of a modified nucleotide can be modified by replacing one or more of the oxygens with a different substituent. Further, the modified nucleotide, e.g., modified nucleotide present in a modified nucleic acid, can include the wholesale replacement of an unmodified phosphate moiety with a modified phosphate as described herein. In some embodiments, the modification of the phosphate backbone can include alterations that result in either an uncharged linker or a charged linker with unsymmetrical charge distribution.

Examples of modified phosphate groups include, phosphorothioate,

phosphoroselenates, borano phosphates, borano phosphate esters, hydrogen phosphonates, phosphoroamidates, alkyl or aryl phosphonates and phosphotriesters. In some embodiments, one of the non-bridging phosphate oxygen atoms in the phosphate backbone moiety can be replaced by any of the following groups: sulfur (S), selenium (Se), BR₃ (wherein R can be, e.g., hydrogen, alkyl, or aryl), C (e.g., an alkyl group, an aryl group, and the like), H, NR₂ (wherein R can be, e.g., hydrogen, alkyl, or aryl), or OR (wherein R can be, e.g., alkyl or aryl). The phosphorous atom in an unmodified phosphate group is achiral. However, replacement of one of the non-bridging oxygens with one of the above atoms or groups of atoms can render the phosphorous atom chiral; that is to say that a phosphorous atom in a phosphate group modified in this way is a stereogenic center. The stereogenic phosphorous atom can possess either the "R" configuration (herein Rp) or the "S" configuration (herein Sp).

Phosphorodithioates have both non-bridging oxygens replaced by sulfur. The phosphorus center in the phosphorodithioates is achiral which precludes the formation of oligoribonucleotide diastereomers. In some embodiments, modifications to one or both non- bridging oxygens can also include the replacement of the non-bridging oxygens with a group independently selected from S, Se, B, C, H, N, and OR (R can be, e.g., alkyl or aryl).

The phosphate linker can also be modified by replacement of a bridging oxygen, (i.e., the oxygen that links the phosphate to the nucleoside), with nitrogen (bridged

phosphoroamidates), sulfur (bridged phosphorothioates) and carbon (bridged

methylenephosphonates). The replacement can occur at either linking oxygen or at both of the linking oxygens.

Replacement of the Phosphate Group

The phosphate group can be replaced by non-phosphorus containing connectors. In some embodiments, the charge phosphate group can be replaced by a neutral moiety.

Examples of moieties which can replace the phosphate group can include, without limitation, e.g., methyl phosphonate, hydroxylamino, siloxane, carbonate, carboxymethyl, carbamate, amide, thioether, ethylene oxide linker, sulfonate, sulfonamide, thioformacetal, formacetal, oxime, methyleneimino, methylenemethylimino, methylenehydrazo,

methylenedimethylhydrazo and methyleneoxymethylimino.

Replacement of the Ribophosphate Backbone

Scaffolds that can mimic nucleic acids can also be constructed wherein the phosphate linker and ribose sugar are replaced by nuclease resistant nucleoside or nucleotide surrogates. In some embodiments, the nucleobases can be tethered by a surrogate backbone. Examples can include, without limitation, the morpholino, cyclobutyl, pyrrolidine and peptide nucleic acid (PNA) nucleoside surrogates.

Sugar Modifications

The modified nucleosides and modified nucleotides can include one or more modifications to the sugar group. For example, the 2' hydroxyl group (OH) can be modified or replaced with a number of different "oxy" or "deoxy" substituents. In some embodiments, modifications to the 2' hydroxyl group can enhance the stability of the nucleic acid since the hydroxyl can no longer be deprotonated to form a 2'-alkoxide ion. The 2'-alkoxide can catalyze degradation by intramolecular nucleophilic attack on the linker phosphorus atom.

Examples of "oxy"-2' hydroxyl group modifications can include alkoxy or aryloxy

(OR, wherein "R" can be, e.g., alkyl, cycloalkyl, aryl, aralkyl, heteroaryl or a sugar);

polyethyleneglycols (PEG), 0(CH₂CH₂0)_nCH₂CH₂OR wherein R can be, e.g., H or optionally substituted alkyl, and n can be an integer from 0 to 20 (e.g., from 0 to 4, from 0 to 8, from 0 to 10, from 0 to 16, from 1 to 4, from 1 to 8, from 1 to 10, from 1 to 16, from 1 to 20, from 2 to 4, from 2 to 8, from 2 to 10, from 2 to 16, from 2 to 20, from 4 to 8, from 4 to 10, from 4 to 16, and from 4 to 20). In some embodiments, the "oxy"-2' hydroxyl group modification can include "locked" nucleic acids (LNA) in which the 2' hydroxyl can be connected, e.g., by a Ci_6 alkylene or Ci_6 heteroalkylene bridge, to the 4' carbon of the same ribose sugar, where exemplary bridges can include methylene, propylene, ether, or amino bridges; O-amino (wherein amino can be, e.g., NH₂; alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, or diheteroarylamino, ethylenediamine, or polyamino) and aminoalkoxy, 0(CH₂)_n-amino, (wherein amino can be, e.g., NH₂;

alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, or diheteroarylamino, ethylenediamine, or polyamino). In some embodiments, the "oxy"-2' hydroxyl group modification can include the methoxyethyl group (MOE), (OCH₂CH₂OCH₃, e.g., a PEG derivative).

"Deoxy" modifications can include hydrogen (i.e. deoxyribose sugars, e.g., at the overhang portions of partially ds RNA); halo (e.g., bromo, chloro, fluoro, or iodo); amino (wherein amino can be, e.g., NH₂; alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, diheteroarylamino, or amino acid);

NH(CH₂CH₂NH)_nCH₂CH₂-amino (wherein amino can be, e.g., as described herein), - NHC(0)R (wherein R can be, e.g., alkyl, cycloalkyl, aryl, aralkyl, heteroaryl or sugar), cyano; mercapto; alkyl-thio-alkyl; thioalkoxy; and alkyl, cycloalkyl, aryl, alkenyl and alkynyl, which may be optionally substituted with e.g., an amino as described herein.

The sugar group can also contain one or more carbons that possess the opposite stereochemical configuration than that of the corresponding carbon in ribose. Thus, a modified nucleic acid can include nucleotides containing e.g., arabinose, as the sugar. The nucleotide "monomer" can have an alpha linkage at the position on the sugar, e.g., alpha- nucleosides. The modified nucleic acids can also include "abasic" sugars, which lack a nucleobase at C- 1 ' . These abasic sugars can also be further modified at one or more of the constituent sugar atoms. The modified nucleic acids can also include one or more sugars that are in the L form, e.g. L- nucleosides.

Generally, RNA includes the sugar group ribose, which is a 5-membered ring having an oxygen. Exemplary modified nucleosides and modified nucleotides can include, without limitation, replacement of the oxygen in ribose (e.g., with sulfur (S), selenium (Se), or alkylene, such as, e.g., methylene or ethylene); addition of a double bond (e.g., to replace ribose with cyclopentenyl or cyclohexenyl); ring contraction of ribose (e.g., to form a 4- membered ring of cyclobutane or oxetane); ring expansion of ribose (e.g., to form a 6- or 7- membered ring having an additional carbon or heteroatom, such as for example,

anhydrohexitol, altritol, mannitol, cyclohexanyl, cyclohexenyl, and morpholino that also has a phosphoramidate backbone). In some embodiments, the modified nucleotides can include multicyclic forms (e.g., tricyclo; and "unlocked" forms, such as glycol nucleic acid (GNA) (e.g., R-GNA or S-GNA, where ribose is replaced by glycol units attached to phosphodiester bonds), threose nucleic acid (TNA, where ribose is replaced with a-L-threofuranosyl- (3'→2')).

Modifications on the Nucleobase

The modified nucleosides and modified nucleotides described herein, which can be incorporated into a modified nucleic acid, can include a modified nucleobase. Examples of nucleobases include, but are not limited to, adenine (A), guanine (G), cytosine (C), and uracil (U). These nucleobases can be modified or wholly replaced to provide modified nucleosides and modified nucleotides that can be incorporated into modified nucleic acids. The nucleobase of the nucleotide can be independently selected from a purine, a pyrimidine, a purine or pyrimidine analog. In some embodiments, the nucleobase can include, for example, naturally-occurring and synthetic derivatives of a base.

Uracil

In some embodiments, the modified nucleobase is a modified uracil. Exemplary nucleobases and nucleosides having a modified uracil include without limitation

pseudouridine (ψ), pyridin-4-one ribonucleoside, 5-aza-uridine, 6-aza-uridine, 2-thio-5-aza- uridine, 2-thio-uridine (s2U), 4-thio-uridine (s4U), 4-thio-pseudouridine, 2-thio- pseudouridine, 5 -hydroxy-uridine (ho⁵U), 5-aminoallyl-uridine, 5-halo-uridine (e.g., 5-iodo- uridine or 5-bromo-uridine), 3-methyl-uridine (m³U), 5-methoxy-uridine (mo⁵U), uridine 5- oxyacetic acid (cmo⁵U), uridine 5-oxyacetic acid methyl ester (mcmo⁵U), 5-carboxymethyl- uridine (cm⁵U), 1-carboxymethyl-pseudouridine, 5-carboxyhydroxymethyl-uridine (chm⁵U), 5-carboxyhydroxymethyl-uridine methyl ester (mchm⁵U), 5-methoxycarbonylmethyl-uridine (mcm⁵U), 5-methoxycarbonylmethyl-2-thio-uridine (mcm⁵s2U), 5-aminomethyl-2-thio- uridine (nm⁵s2U), 5-methylaminomethyl-uridine (mnm⁵U), 5-methylaminomethyl-2-thio- uridine (mnm⁵s2U), 5-methylaminomethyl-2-seleno-uridine (mnm⁵se²U), 5- carbamoylmethyl-uridine (ncm⁵U), 5-carboxymethylaminomethyl-uridine (cmnm⁵U), 5- carboxymethylaminomethyl-2-thio-uridine (cmnm ⁵s2U), 5 -propynyl -uridine, 1-propynyl- pseudouridine, 5-taurinomethyl-uridine (tcm⁵U), 1-taurinomethyl-pseudouridine, 5- taurinomethyl-2-thio-uridine(Tm⁵s2U), l-taurinomethyl-4-thio-pseudouridine, 5-methyl- uridine (m⁵U, i.e., having the nucleobase deoxy thy mine), 1 -methyl -pseudouridine (ηι ψ), 5- methyl-2-thio-uridine (m⁵s2U), l-methyl-4-thio-pseudouridine (m vi/), 4-thio-l-methyl- pseudouridine, 3-methyl-pseudouridine (ιη³ψ), 2-thio- 1-methyl-pseudouridine, 1-methyl-l- deaza-pseudouridine, 2-thio-l-methyl-l-deaza-pseudouridine, dihydrouridine (D), dihydropseudouridine, 5,6-dihydrouridine, 5-methyl-dihydrouridine (m⁵D), 2-thio- dihydrouridine, 2-thio-dihydropseudouridine, 2-methoxy-uridine, 2-methoxy-4-thio-uridine, 4-methoxy-pseudouridine, 4-methoxy-2-thio-pseudouridine, N 1-methyl-pseudouridine, 3-(3- amino-3-carboxypropyl)uridine (acp³U), l-methyl-3-(3-amino-3- carboxypropyl)pseudouridine

5-(isopentenylaminomethyl)uridine (inm⁵U), 5- (isopentenylaminomethyl)-2-thio-uridine (inm⁵s2U), a-thio-uridine, 2'-0-methyl-uridine (Um), 5,2'-0-dimethyl-uridine (m⁵Um), 2'-0-methyl-pseudouridine (ψηι), 2-thio-2'-0- methyl-uridine (s2Um), 5-methoxycarbonylmethyl-2'-0-methyl-uridine (mcm ⁵Um), 5- carbamoylmethyl-2'-0-methyl-uridine (ncm ⁵Um), 5-carboxymethylaminomethyl-2'-0- methyl-uridine (cmnm ⁵Um), 3,2'-0-dimethyl-uridine (m³Um), 5-(isopentenylaminomethyl)- 2'-0-methyl-uridine (inm ⁵Um), 1-thio-uridine, deoxythymidine, 2'-F-ara-uridine, 2'-F- uridine, 2'-OH-ara-uridine, 5-(2-carbomethoxyvinyl) uridine, 5-[3-(l-E- propenylamino)uridine, pyrazolo[3,4-d]pyrimidines, xanthine, and hypoxanthine.

Cytosine

In some embodiments, the modified nucleobase is a modified cytosine. Exemplary nucleobases and nucleosides having a modified cytosine include without limitation 5-aza- cytidine, 6-aza-cytidine, pseudoisocytidine, 3-methyl-cytidine (m³C), N4-acetyl-cytidine (act), 5-formyl-cytidine (f⁵C), N4-methyl-cytidine (m⁴C), 5-methyl-cytidine (m⁵C), 5-halo- cytidine (e.g., 5-iodo-cytidine), 5-hydroxymethyl-cytidine (hm⁵C), 1-methyl- pseudoisocytidine, pyrrolo-cytidine, pyrrolo-pseudoisocytidine, 2-thio-cytidine (s2C), 2-thio- 5-methyl-cytidine, 4-thio-pseudoisocytidine, 4-thio-l-methyl-pseudoisocytidine, 4-thio-l- methyl-l-deaza-pseudoisocytidine, 1 -methyl- 1-deaza-pseudoisocytidine, zebularine, 5-aza- zebularine, 5-methyl-zebularine, 5-aza-2-thio-zebularine, 2-thio-zebularine, 2-methoxy- cytidine, 2-methoxy-5-methyl-cytidine, 4-methoxy-pseudoisocytidine, 4-methoxy-l -methyl - pseudoisocytidine, lysidine (k²C), a-thio-cytidine, 2'-0-methyl-cytidine (Cm), 5,2'-0- dimethyl-cytidine (m⁵Cm), N4-acetyl-2'-0-methyl-cytidine (ac⁴Cm), N4,2'-0-dimethyl- cytidine (m⁴Cm), 5-formyl-2'-0-methyl-cytidine (f ⁵Cm), N4,N4,2'-0-trimethyl-cytidine (m⁴ ₂Cm), 1-thio-cytidine, 2'-F-ara-cytidine, 2'-F-cytidine, and 2'-OH-ara-cytidine.

Adenine In some embodiments, the modified nucleobase is a modified adenine. Exemplary nucleobases and nucleosides having a modified adenine include without limitation 2-amino- purine, 2,6-diaminopurine, 2-amino-6-halo-purine (e.g., 2-amino-6-chloro-purine), 6-halo- purine (e.g., 6-chloro-purine), 2-amino-6-methyl-purine, 8-azido-adenosine, 7-deaza- adenosine, 7-deaza-8-aza-adenosine, 7-deaza-2-amino-purine, 7-deaza-8-aza-2-amino-purine, 7-deaza-2,6-diaminopurine, 7-deaza-8-aza-2,6-diaminopurine, 1-methyl-adenosine (m^]A), 2- methyl-adenosine (m²A), N6-methyl-adenosine (m⁶A), 2-methylthio-N6-methyl-adenosine (ms2m⁶A), N6-isopentenyl-adenosine (i⁶A), 2-methylthio-N6-isopentenyl-adenosine

(ms²i⁶A), N6-(cis-hydroxyisopentenyl)adenosine (io⁶A), 2-methylthio-N6-(cis- hydroxyisopentenyl)adenosine (ms2io⁶A), N6-glycinylcarbamoyl-adenosine (g⁶A), N6- threonylcarbamoyl-adenosine (t⁶A), N6-methyl-N6-threonylcarbamoyl-adenosine (m⁶t⁶A), 2- methylthio-N6-threonylcarbamoyl-adenosine (ms²g⁶A), N6,N6-dimethyl-adenosine (m⁶ ₂A), N6-hydroxynorvalylcarbamoyl-adenosine (hn⁶A), 2-methylthio-N6- hydroxynorvalylcarbamoyl-adenosine (ms2hn⁶A), N6-acetyl-adenosine (ac⁶A), 7-methyl- adenosine, 2-methylthio-adenosine, 2-methoxy-adenosine, a-thio-adenosine, 2'-0-methyl- adenosine (Am), N⁶,2'-0-dimethyl-adenosine (m⁶Am), N⁶-Methyl-2'-deoxyadenosine, N6,N6,2'-0-trimethyl-adenosine (m⁶ ₂Am), l,2'-0-dimethyl-adenosine (tt^Am), 2'-0- ribosyladenosine (phosphate) (Ar(p)), 2-amino-N6-methyl -purine, 1-thio-adenosine, 8-azido- adenosine, 2'-F-ara-adenosine, 2'-F-adenosine, 2'-OH-ara-adenosine, and N6-(19-amino- pentaoxanonadecyl)-adenosine.

Guanine

In some embodiments, the modified nucleobase is a modified guanine. Exemplary nucleobases and nucleosides having a modified guanine include without limitation inosine (I), 1-methyl-inosine (m^]I), wyosine (imG), methylwyosine (mimG), 4-demethyl-wyosine (imG- 14), isowyosine (imG2), wybutosine (yW), peroxywybutosine (o₂yW),

hydroxywybutosine (OHyW), undermodified hydroxywybutosine (OHyW*), 7-deaza- guanosine, queuosine (Q), epoxyqueuosine (oQ), galactosyl-queuosine (galQ), mannosyl- queuosine (manQ), 7-cyano-7-deaza-guanosine (preQo), 7-aminomethyl-7-deaza-guanosine (preQi), archaeosine (G⁺), 7-deaza-8-aza-guanosine, 6-thio-guanosine, 6-thio-7-deaza- guanosine, 6-thio-7-deaza-8-aza-guanosine, 7-methyl-guanosine (m⁷G), 6-thio-7-methyl- guanosine, 7-methyl-inosine, 6-methoxy-guanosine, 1-methyl-guanosine (m'G), N2-methyl- guanosine (m²G), N2,N2-dimethyl-guanosine (m² ₂G), N2,7-dimethyl-guanosine (m²,7G), N2, N2,7-dimethyl-guanosine (m²,2,7G), 8-oxo-guanosine, 7-methyl-8-oxo-guanosine, 1- methyl-6-thio-guanosine, N2-methyl-6-thio-guanosine, N2,N2-dimethyl-6-thio-guanosine, a- thio-guanosine, 2'-0-methyl-guanosine (Gm), N2-methyl-2'-0-methyl-guanosine (m²Gm), N2,N2-dimethyl-2'-0-methyl-guanosine (m² ₂Gm), l-methyl-2'-0-methyl-guanosine (m'Gm), N2,7-dimethyl-2'-0-methyl-guanosine (m²,7Gm), 2'-0-methyl -inosine (Im), l,2'-0-dimethyl- inosine (m'lm), 0⁶-phenyl-2'-deoxyinosine, 2'-0-ribosylguanosine (phosphate) (Gr(p)), 1- thio-guanosine, 0⁶-methyl-guanosine, 0⁶-Methyl-2'-deoxy guanosine, 2'-F-ara-guanosine, and 2'-F-guanosine.

Exemplary Modified gRNAs

In some embodiments, the modified nucleic acids can be modified gRNAs. It is to be understood that any of the gRNAs described herein can be modified in accordance with this section, including any gRNA that comprises a targeting domain from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A- 12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

As discussed above, transiently expressed or delivered nucleic acids can be prone to degradation by, e.g., cellular nucleases. Accordingly, in one aspect the modified gRNAs described herein can contain one or more modified nucleosides or nucleotides which introduce stability toward nucleases. While not wishing to be bound by theory it is also believed that certain modified gRNAs described herein can exhibit a reduced innate immune response when introduced into a population of cells, particularly the cells of the present invention. As noted above, the term "innate immune response" includes a cellular response to exogenous nucleic acids, including single stranded nucleic acids, generally of viral or bacterial origin, which involves the induction of cytokine expression and release, particularly the interferons, and cell death.

While some of the exemplary modification discussed in this section may be included at any position within the gRNA sequence, in some embodiments, a gRNA comprises a modification at or near its 5' end (e.g., within 1-10, 1-5, or 1-2 nucleotides of its 5' end). In some embodiments, a gRNA comprises a modification at or near its 3' end (e.g., within 1-10, 1-5, or 1-2 nucleotides of its 3' end). In some embodiments, a gRNA comprises both a modification at or near its 5' end and a modification at or near its 3' end.

In an embodiment, the 5 ' end of a gRNA is modified by the inclusion of a eukaryotic mRNA cap structure or cap analog (e.g., a G(5')ppp(5')G cap analog, a m7G(5')ppp(5')G cap analog, or a 3'-0-Me-m7G(5')ppp(5')G anti reverse cap analog (ARCA)). The cap or cap analog can be included during either chemical synthesis or in vitro transcription of the gRNA. In an embodiment, an in vitro transcribed gRNA is modified by treatment with a phosphatase (e.g., calf intestinal alkaline phosphatase) to remove the 5' triphosphate group.

In an embodiment, the 3 ' end of a gRNA is modified by the addition of one or more (e.g., 25-200) adenine (A) residues. The polyA tract can be contained in the nucleic acid (e.g., plasmid, PCR product, viral genome) encoding the gRNA, or can be added to the gRNA during chemical synthesis, or following in vitro transcription using a polyadenosine polymerase (e.g., E. coli Poly(A)Polymerase).

In an embodiment, in vitro transcribed gRNA contains both a 5 ' cap structure or cap analog and a 3' polyA tract. In an embodiment, an in vitro transcribed gRNA is modified by treatment with a phosphatase (e.g., calf intestinal alkaline phosphatase) to remove the 5' triphosphate group and comprises a 3' polyA tract.

In some embodiments, gRNAs can be modified at a 3' terminal U ribose. For example, the two terminal hydroxyl groups of the U ribose can be oxidized to aldehyde groups and a concomitant opening of the ribose ring to afford a modified nucleoside as shown below:

wherein "U" can be an unmodified or modified uridine.

In another embodiment, the 3' terminal U can be modified with a 2' 3' cyclic phosphate as shown below:

wherein "U" can be an unmodified or modified uridine.

In some embodiments, the gRNA molecules may contain 3' nucleotides which can be stabilized against degradation, e.g., by incorporating one or more of the modified nucleotides described herein. In this embodiment, e.g., uridines can be replaced with modified uridines, e.g., 5-(2-amino)propyl uridine, and 5-bromo uridine, or with any of the modified uridines described herein; adenosines and guanosines can be replaced with modified adenosines and guanosines, e.g., with modifications at the 8-position, e.g., 8-bromo guanosine, or with any of the modified adenosines or guanosines described herein.

In some embodiments, sugar-modified ribonucleotides can be incorporated into the gRNA, e.g., wherein the 2' OH-group is replaced by a group selected from H, -OR, -R (wherein R can be, e.g., alkyl, cycloalkyl, aryl, aralkyl, heteroaryl or sugar), halo, -SH, -SR (wherein R can be, e.g., alkyl, cycloalkyl, aryl, aralkyl, heteroaryl or sugar), amino (wherein amino can be, e.g., NH₂; alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, diheteroarylamino, or amino acid); or cyano (-CN). In some embodiments, the phosphate backbone can be modified as described herein, e.g., with a phosphothioate group. In some embodiments, one or more of the nucleotides of the gRNA can each independently be a modified or unmodified nucleotide including, but not limited to 2 '-sugar modified, such as, 2'-0-methyl, 2'-0-methoxyethyl, or 2'-Fluoro modified including, e.g., 2'-F or 2'-0-methyl, adenosine (A), 2'-F or 2'-0-methyl, cytidine (C), 2'-F or 2'-0-methyl, uridine (U), 2'-F or 2'-0-methyl, thymidine (T), 2'-F or 2'-0-methyl, guanosine (G), 2'-0- methoxyethyl-5-methyluridine (Teo), 2'-0-methoxyethyladenosine (Aeo), 2'-0- methoxyethyl-5-methylcytidine (m5Ceo), and any combinations thereof.

In some embodiments, a gRNA can include "locked" nucleic acids (LNA) in which the 2' OH-group can be connected, e.g., by a Cl-6 alkylene or Cl-6 heteroalkylene bridge, to the 4' carbon of the same ribose sugar, where exemplary bridges can include methylene, propylene, ether, or amino bridges; O-amino (wherein amino can be, e.g., NH₂; alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, or diheteroarylamino, ethylenediamine, or polyamino) and aminoalkoxy or 0(CH₂)_n-amino (wherein amino can be, e.g., NH₂; alkylamino, dialkylamino, heterocyclyl, arylamino, diarylamino, heteroarylamino, or diheteroarylamino, ethylenediamine, or polyamino).

In some embodiments, a gRNA can include a modified nucleotide which is multicyclic (e.g., tricyclo; and "unlocked" forms, such as glycol nucleic acid (GNA) (e.g., R- GNA or S-GNA, where ribose is replaced by glycol units attached to phosphodiester bonds), or threose nucleic acid (TNA, where ribose is replaced with a-L-threofuranosyl-(3'→2')).

Generally, gRNA molecules include the sugar group ribose, which is a 5-membered ring having an oxygen. Exemplary modified gRNAs can include, without limitation, replacement of the oxygen in ribose (e.g., with sulfur (S), selenium (Se), or alkylene, such as, e.g., methylene or ethylene); addition of a double bond (e.g., to replace ribose with cyclopentenyl or cyclohexenyl); ring contraction of ribose (e.g., to form a 4-membered ring of cyclobutane or oxetane); ring expansion of ribose (e.g., to form a 6- or 7-membered ring having an additional carbon or heteroatom, such as for example, anhydrohexitol, altritol, mannitol, cyclohexanyl, cyclohexenyl, and morpholino that also has a phosphoramidate backbone). Although the majority of sugar analog alterations are localized to the 2' position, other sites are amenable to modification, including the 4' position. In an embodiment, a gRNA comprises a 4'-S, 4'-Se or a 4'-C-aminomethyl-2'-0-Me modification.

In some embodiments, deaza nucleotides, e.g., 7-deaza-adenosine, can be

incorporated into the gRNA. In some embodiments, O- and N-alkylated nucleotides, e.g., N6-methyl adenosine, can be incorporated into the gRNA. In some embodiments, one or more or all of the nucleotides in a gRNA molecule are deoxynucleotides. miRNA binding sites

microrNAs (or miRNAs) are naturally occurring cellular 19-25 nucleotide long noncoding RNAs. They bind to nucleic acid molecules having an appropriate miRNA binding site, e.g., in the 3' UTR of an mRNA, and down-regulate gene expression. While not wishing to be bound by theory it is believed that the down regulation is either by reducing nucleic acid molecule stability or by inhibiting translation. An RNA species disclosed herein, e.g., an mRNA encoding Cas9 can comprise an miRNA binding site, e.g., in its 3'UTR. The miRNA binding site can be selected to promote down regulation of expression is a selected cell type. By way of example, the incorporation of a binding site for miR-122, a microrNA abundant in liver, can inhibit the expression of the gene of interest in the liver.

EXAMPLES

The following Examples are merely illustrative and are not intended to limit the scope or content of the invention in any way.

Example 1 : Evaluation of candidate guide RNAs (gRNAs)

The suitability of candidate gRNAs can be evaluated as described in this example. Although described for a chimeric gRNA, the approach can also be used to evaluate modular gRNAs.

Cloning gRNAs into Vectors

For each gRNA, a pair of overlapping oligonucleotides is designed and obtained. Oligonucleotides are annealed and ligated into a digested vector backbone containing an upstream U6 promoter and the remaining sequence of a long chimeric gRNA. Plasmid is sequence-verified and prepped to generate sufficient amounts of transfection-quality DNA. Alternate promoters maybe used to drive in vivo transcription (e.g. HI promoter) or for in vitro transcription (e.g., a T7 promoter).

Cloning gRNAs in linear dsDNA molecule (STITCHR)

For each gRNA, a single oligonucleotide is designed and obtained. The U6 promoter and the gRNA scaffold (e.g. including everything except the targeting domain, e.g., including sequences derived from the crRNA and tracrRNA, e.g., including a first complementarity domain; a linking domain; a second complementarity domain; a proximal domain; and a tail domain) are separately PCR amplified and purified as dsDNA molecules. The gRNA- specific oligonucleotide is used in a PCR reaction to stitch together the U6 and the gRNA scaffold, linked by the targeting domain specified in the oligonucleotide. Resulting dsDNA molecule (STITCHR product) is purified for transfection. Alternate promoters may be used to drive in vivo transcription (e.g., HI promoter) or for in vitro transcription (e.g., T7 promoter). Any gRNA scaffold may be used to create gRNAs compatible with Cas9s from any bacterial species.

Initial gRNA Screen

Each gRNA to be tested is transfected, along with a plasmid expressing Cas9 and a small amount of a GFP-expressing plasmid into human cells. In preliminary experiments, these cells can be immortalized human cell lines such as 293T, K562 or U20S.

Alternatively, primary human cells may be used. In this case, cells may be relevant to the eventual therapeutic cell target (for example, an erythroid cell). The use of primary cells similar to the potential therapeutic target cell population may provide important information on gene targeting rates in the context of endogenous chromatin and gene expression.

Transfection may be performed using lipid transfection (such as Lipofectamine or Fugene) or by electroporation (such as Lonza Nucleofection). Following transfection, GFP expression can be determined either by fluorescence microscopy or by flow cytometry to confirm consistent and high levels of transfection. These preliminary transfections can comprise different gRNAs and different targeting approaches (17-mers, 20-mers, nuclease, dual-nickase, etc.) to determine which gRNAs/combinations of gRNAs give the greatest activity.

Efficiency of cleavage with each gRNA may be assessed by measuring NHEJ- induced indel formation at the target locus by a T7El-type assay or by sequencing.

Alternatively, other mismatch-sensitive enzymes, such as Cell/Surveyor nuclease, may also be used. For the T7E1 assay, PCR amplicons are approximately 500-700bp with the intended cut site placed asymmetrically in the amplicon. Following amplification, purification and size- verification of PCR products, DNA is denatured and re-hybridized by heating to 95 °C and then slowly cooling. Hybridized PCR products are then digested with T7 Endonuclease I (or other mismatch-sensitive enzyme) which recognizes and cleaves non-perfectly matched DNA. If indels are present in the original template DNA, when the amplicons are denatured and re-annealed, this results in the hybridization of DNA strands harboring different indels and therefore lead to double-stranded DNA that is not perfectly matched. Digestion products may be visualized by gel electrophoresis or by capillary electrophoresis. The fraction of DNA that is cleaved (density of cleavage products divided by the density of cleaved and uncleaved) may be used to estimate a percent NHEJ using the following equation: NHEJ = (1-(1 -fraction cleaved)'⁷²). The T7E1 assay is sensitive down to about 2-5% NHEJ.

Sequencing may be used instead of, or in addition to, the T7E1 assay. For Sanger sequencing, purified PCR amplicons are cloned into a plasmid backbone, transformed, miniprepped and sequenced with a single primer. Sanger sequencing may be used for determining the exact nature of indels after determining the NHEJ rate by T7E1.

Sequencing may also be performed using next generation sequencing techniques. When using next generation sequencing, amplicons may be 300-500bp with the intended cut site placed asymmetrically. Following PCR, next generation sequencing adapters and barcodes (for example Illumina multiplex adapters and indexes) may be added to the ends of the amplicon, e.g., for use in high throughput sequencing (for example on an Illumina MiSeq). This method allows for detection of very low NHEJ rates.

Example 2: Assessment of Gene Targeting by NHEJ

The gRNAs that induce the greatest levels of NHEJ in initial tests can be selected for further evaluation of gene targeting efficiency. In this case, cells are derived from disease subjects and, therefore, harbor the relevant mutation.

Following transfection (usually 2-3 days post-transfection,) genomic DNA may be isolated from a bulk population of transfected cells and PCR may be used to amplify the target region. Following PCR, gene targeting efficiency to generate the desired mutations (either knockout of a target gene or removal of a target sequence motif) may be determined by sequencing. For Sanger sequencing, PCR amplicons may be 500-700bp long. For next generation sequencing, PCR amplicons may be 300-500bp long. If the goal is to knockout gene function, sequencing may be used to assess what percent of viral copies have undergone NHEJ-induced indels that result in a frameshift or large deletion or insertion that would be expected to destroy gene function. If the goal is to remove a specific sequence motif, sequencing may be used to assess what percent of viral copies have undergone NHEJ- induced deletions that span this sequence.

Incorporation by Reference

All publications, patents, and patent applications mentioned herein are hereby incorporated by reference in their entirety as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference. In case of conflict, the present application, including any definitions herein, will control.

Equivalents

Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

CLAIMS What is claimed is:

1. A gRNA molecule comprising a targeting domain which is complementary with a target domain from the UL19, UL30, UL48 or UL54 gene.

2. The gRNA molecule of claim 1 , wherein said targeting domain is configured to

provide a cleavage event selected from a double strand break and a single strand break, within 500, 400, 300, 200, 100, 50, 25, or 10 nucleotides of a HSV-2 target position.

3. The gRNA molecule of claim 1 or 2, wherein said targeting domain is complementary with a target domain from the UL19 gene.

4. The gRNA molecule of claim 1 or 2, wherein said targeting domain is complementary with a target domain from the UL30 gene.

5. The gRNA molecule of claim 1 or 2, wherein said targeting domain is complementary with a target domain from the UL48 gene.

6. The gRNA molecule of claim 1 or 2, wherein said targeting domain is complementary with a target domain from the UL54 gene.

7. The gRNA molecule of any of claims 1-6, wherein said targeting domain is

configured to target the coding region, optionally an early coding region, of the UL19, UL30, UL48 or UL54 gene.

8. The gRNA molecule of any of claims 1-7, wherein said targeting domain comprises a sequence that is the same as, or differs by no more than 3 nucleotides from, a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A- 5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A- 14E, 15A-15G, or 16A-16C.

9. The gRNA molecule of any of claims 1-7, wherein said targeting domain comprises a sequence that is the same as a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

10. The gRNA molecule of any of claims 1-9, wherein said gRNA is a modular gRNA molecule.

11. The gRNA molecule of any of claims 1-9, wherein said gRNA is a chimeric gRNA molecule.

12. The gRNA molecule of any of claims 1-11, wherein said targeting domain is 16

nucleotides or more in length.

13. The gRNA molecule of any of claims 1-12, wherein said targeting domain is 17 or 18 nucleotides or morein length.

14. The gRNA molecule of any of claims 1-13, wherein said targeting domain is 19 nucleotides or more in length.

15. The gRNA molecule of any of claims 1-14, wherein said targeting domain is 20 nucleotides or more in length.

16. The gRNA molecule of any of claims 1-15, wherein said targeting domain is 21 nucleotides or more in length.

17. The gRNA molecule of any of claims 1-16, wherein said targeting domain is 22 nucleotides or more in length.

18. The gRNA molecule of any of claims 1-17, wherein said targeting domain is 23 nucleotides or more in length.

19. The gRNA molecule of any of claims 1-18, wherein said targeting domain is 24 nucleotides or more in length.

20. The gRNA molecule of any of claims 1-19, wherein said targeting domain is 25 nucleotides or more in length.

21. The gRNA molecule of any of claims 1-20, wherein said targeting domain is 26 nucleotides or more in length.

22. The gRNA molecule of any of claims 1-21, comprising from 5' to 3' :

a targeting domain;

a first complementarity domain;

a linking domain;

a second complementarity domain;

a proximal domain; and

a tail domain.

23. The gRNA molecule of any of claims 1-21, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and

a targeting domain of 17 or 18 nucleotides in length.

24. The gRNA molecule of any of claims 1-23, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 25 nucleotides in length; and a targeting domain of 17 or 18 nucleotides in length.

25. The gRNA molecule of any of claims 1-24, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

26. The gRNA molecule of any of claims 1-25, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

27. A nucleic acid that comprises: (a) sequence that encodes a gRNA molecule

comprising a targeting domain that is complementary with a HSV-2 target domain in a UL19, UL30, UL48 or UL54 gene.

28. The nucleic acid of claim 27, wherein said gRNA molecule is a gRNA molecule of any of claims 1-26.

29. The nucleic acid of claim 27 or 28, wherein said targeting domain is configured to provide a cleavage event selected from a double strand break and a single strand break, within 500, 400, 300, 200, 100, 50, 25, or 10 nucleotides of the HSV-2 target position.

30. The nucleic acid of any of claims 27-29, wherein said targeting domain is configured to target the coding region, optionally an early coding region, of the UL19, UL30, UL48 or UL54 gene.

31. The nucleic acid of any of claims 27-30, wherein said targeting domain comprises a sequence that is the same as, or differs by no more than 3 nucleotides from, a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A- 5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A- 14E, 15A-15G, or 16A-16C.

32. The nucleic acid of any of claims 27-30, wherein said targeting domain comprises a sequence that is the same as a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

33. The nucleic acid of any of claims 27-32, wherein said gRNA is a modular gRN molecule.

34. The nucleic acid of any of claims 27-32, wherein said gRNA is a chimeric gRN molecule.

35. The nucleic acid of any of claims 27-34, wherein said targeting domain is 16 nucleotides or more in length.

36. The nucleic acid of any of claims 32-35, wherein said targeting domain is 17 or nucleotides or more in length.

37. The nucleic acid of any of claims 32-36, wherein said targeting domain is 19 nucleotides or more in length.

38. The nucleic acid of any of claims 32-37, wherein said targeting domain is 20 nucleotides or more in length.

39. The nucleic acid of any of claims 32-38, wherein said targeting domain is 21 nucleotides or more in length.

40. The nucleic acid of any of claims 32-39, wherein said targeting domain is 22 nucleotides or more in length.

41. The nucleic acid of any of claims 32-40, wherein said targeting domain is 23 nucleotides or more in length.

42. The nucleic acid of any of claims 32-41, wherein said targeting domain is 24 nucleotides or more in length.

43. The nucleic acid of any of claims 32-42, wherein said targeting domain is 25 nucleotides or more in length.

44. The nucleic acid of any of claims 32-43, wherein said targeting domain is 26 nucleotides or more in length.

45. The nucleic acid of any of claims 27-44, comprising from 5' to 3':

a targeting domain;

a first complementarity domain;

a linking domain;

a second complementarity domain;

a proximal domain; and

a tail domain.

46. The nucleic acid of any of claims 27-45, comprising:

a linking domain of no more than 25 nucleotides in length; a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and

a targeting domain of 17 or 18 nucleotides in length.

47. The nucleic acid of any of claims 27-46, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 25 nucleotides in length; and

a targeting domain of 17 or 18 nucleotides in length.

48. The nucleic acid of any of claims 27-47, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

49. The nucleic acid of any of claims 27-48, comprising:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

50. The nucleic acid of any of claims 27-49, further comprising: (b) sequence that

encodes a Cas9 molecule.

51. The nucleic acid of claim 50, wherein said Cas9 molecule is an eaCas9 molecule.

52. The nucleic acid of claim 51 , wherein said eaCas9 molecule comprises a nickase molecule.

53. The nucleic acid of claim 51, wherein said eaCas9 molecule forms a double strand break in a target nucleic acid.

54. The nucleic acid of any of claims 51, or 52, wherein said eaCas9 molecule forms a single strand break in a target nucleic acid.

55. The nucleic acid of any of claims 51, 52 or 54, wherein said single strand break is formed in the strand of the target nucleic acid to which the targeting domain of said gRNA molecule is complementary.

56. The nucleic acid of any of claims 51, 52 or 54, wherein said single strand break is formed in the strand of the target nucleic acid other than the strand to which to which the targeting domain of said gRNA is complementary.

57. The nucleic acid of any of claims 51, 52 or 54, wherein said eaCas9 molecule comprises HNH-like domain cleavage activity but has no, or no significant, N- terminal RuvC-like domain cleavage activity.

58. The nucleic acid of any of claims 51, 52, 54, or 57, wherein said eaCas9 molecule is an HNH-like domain nickase.

59. The nucleic acid of any of claims 51, 52, 54, 57, or 58, wherein said eaCas9 molecule comprises a mutation at D10.

60. The nucleic acid of any of claims 51, 52 or 54, wherein said eaCas9 molecule

comprises N-terminal RuvC-like domain cleavage activity but has no, or no significant, HNH-like domain cleavage activity.

61. The nucleic acid of any of claims 51, 52, 54, or 60, wherein said eaCas9 molecule is an N-terminal RuvC-like domain nickase.

62. The nucleic acid of any of claims 51, 52, 54, 60, or 61, wherein said eaCas9 molecule comprises a mutation at H840 or N863.

63. The nucleic acid of any of claims 27-62, further comprising: (c) sequence that

encodes a second gRNA molecule described herein having a targeting domain that is complementary to a second target domain of the UL19, UL30, UL48 or UL54 gene.

64. The nucleic acid of claim 63, wherein said second gRNA molecule is a gRNA

molecule of any of claims 1-26.

65. The nucleic acid of claim 63 or 64, wherein said targeting domain of said second gRNA is configured to provide a cleavage event selected from a double strand break and a single strand break, within 500, 400, 300, 200, 100, 50, 25, or 10 nucleotides of the HSV-2 target position.

66. The nucleic acid of any of claims 63-65, wherein said targeting domain of said second gRNA is configured to target the coding region, optionally an early coding region, of the UL19, UL30, UL48 or UL54 gene.

67. The nucleic acid of any of claims 63-66, wherein said targeting domain of said second gRNA comprises a sequence that is the same as, or differs by no more than 3 nucleotides from, a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A- 12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

68. The nucleic acid of any of claims 63-66, wherein said targeting domain of said second gRNA comprises a sequence that is the same as a targeting domain sequence from any of Tables 1A-1G, 2A-2F, 3A-3F, 4A-4G, 5A-5E, 6A-6G, 7A-7D, 8A-8E, 9A-9G, 10A-10D, 11A-11E, 12A-12G, 13A-13B, 14A-14E, 15A-15G, or 16A-16C.

69. The nucleic acid of any of claims 63-68, wherein said second gRNA molecule is a modular gRNA molecule.

70. The nucleic acid of any of claims 63-68, wherein said second gRNA molecule is a chimeric gRNA molecule.

71. The nucleic acid of any of claims 63-70, wherein said targeting domain of the second gRNA molecule is 16 nucleotides or more in length.

72. The nucleic acid of any of claims 63-71, wherein said targeting domain of the second gRNA molecule is 17 or 18 nucleotides or more in length.

73. The nucleic acid of any of claims 63-72, wherein said targeting domain of the second gRNA molecule is 19 nucleotidesor more in length.

74. The nucleic acid of any of claims 63-73, wherein said targeting domain of the second gRNA molecule is 20 nucleotides or more in length.

75. The nucleic acid of any of claims 63-74, wherein said targeting domain of the second gRNA molecule is 21 nucleotides or more in length.

76. The nucleic acid of any of claims 63-75, wherein said targeting domain of the second gRNA molecule is 22 nucleotides or more in length.

77. The nucleic acid of any of claims 63-76, wherein said targeting domain of the second gRNA molecule is 23 nucleotides or more in length.

78. The nucleic acid of any of claims 63-77, wherein said targeting domain of the second gRNA molecule is 24 nucleotides or more in length.

79. The nucleic acid of any of claims 63-78, wherein said targeting domain of the second gRNA molecule is 25 nucleotides or more in length.

80. The nucleic acid of any of claims 63-79, wherein said targeting domain of the second gRNA molecule is 26 nucleotides or more in length.

81. The nucleic acid of any of claims 63-80, wherein said second gRNA molecule

comprises from 5' to 3' :

a targeting domain;

a first complementarity domain;

a linking domain;

a second complementarity domain;

a proximal domain; and

a tail domain.

82. The nucleic acid of any of claims 63-81, wherein said second gRNA molecule comprises:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 20 nucleotides in length; and

a targeting domain of 17 or 18 nucleotides in length.

83. The nucleic acid of any of claims 63-82, wherein said second molecule gRNA

molecule comprises:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 25 nucleotides in length; and

a targeting domain of 17 or 18 nucleotides in length.

84. The nucleic acid of any of claims 63-83, wherein said second gRNA molecule

comprises:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 30 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

85. The nucleic acid of any of claims 63-84, wherein said second gRNA molecule

comprises:

a linking domain of no more than 25 nucleotides in length;

a proximal and tail domain, that taken together, are at least 40 nucleotides in length; and

a targeting domain of 17 nucleotides in length.

86. The nucleic acid of any of claims 63-85, further comprising a third gRNA molecule.

87. The nucleic acid of claim 86, further comprising a fourth gRNA molecule.

88. The nucleic acid of any of claims 27-62, wherein said nucleic acid does not comprise (c) a sequence that encodes a second gRNA molecule.

89. The nucleic acid of any of claims 50-88, wherein each of (a) and (b) is present on the same nucleic acid molecule.

90. The nucleic acid of claim 89, wherein said nucleic acid molecule is an AAV vector.

91. The nucleic acid of any of claims 50-88, wherein: (a) is present on a first nucleic acid molecule; and (b) is present on a second nucleic acid molecule.

92. The nucleic acid of claim 91, wherein said first and second nucleic acid molecules are AAV vectors.

93. The nucleic acid of any of claims 63-87 or 89-92, wherein each of (a) and (c) is present on the same nucleic acid molecule.

94. The nucleic acid of claim 93, wherein said nucleic acid molecule is an AAV vector.

95. The nucleic acid of any of claims 63-87 or 89-92, wherein: (a) is present on a first nucleic acid molecule; and (c) is present on a second nucleic acid molecule.

96. The nucleic acid of claim 95, wherein said first and second nucleic acid molecules are AAV vectors.

97. The nucleic acid of any of claims 72-87, 89, 90, 93, or 94, wherein each of (a), (b), and (c) are present on the same nucleic acid molecule.

98. The nucleic acid of claim 97, wherein said nucleic acid molecule is an AAV vector.

99. The nucleic acid of any of claims 27-87 or 89-96, wherein:

one of (a), (b), and (c) is encoded on a first nucleic acid molecule; and

a second and third of (a), (b), and (c) is encoded on a second nucleic acid molecule.

100. The nucleic acid of claim 99, wherein said first and second nucleic acid

molecules are AAV vectors.

101. The nucleic acid of any of claims 27-87, 91, 92, 95, 96, 99, or 100, wherein:

(a) is present on a first nucleic acid molecule; and (b) and (c) are present on a second nucleic acid molecule.

102. The nucleic acid of claim 101, wherein said first and second nucleic acid molecules are AAV vectors.

103. The nucleic acid of any of claims 27-87, 91-94, 99, or 100, wherein: (b) is present on a first nucleic acid molecule; and (a) and (c) are present on a second nucleic acid molecule.

104. The nucleic acid of claim 103, wherein said first and second nucleic acid molecules are AAV vectors.

105. The nucleic acid of any of claims 27-87, 89, 90, 95, 96, 99, or 100, wherein:

(c) is present on a first nucleic acid molecule; and (b) and (a) are present on a second nucleic acid molecule.

106. The nucleic acid of claim 105, wherein said first and second nucleic acid molecules are AAV vectors.

107. The nucleic acid of any of claims 91, 95, 99, 101, 103, or 105, wherein said first nucleic acid molecule is other than an AAV vector and said second nucleic acid molecule is an AAV vector.

108. The nucleic acid of any of claims 27-107, wherein said nucleic acid comprises a promoter operably linked to the sequence that encodes said gRNA molecule of (a).

109. The nucleic acid of any of claims 63-87 or 89-108, wherein said nucleic acid comprises a second promoter operably linked to the sequence that encodes the second gRNA molecule of (c).

110. The nucleic acid of claims 109, wherein the promoter and second promoter differ from one another.

111. The nucleic acid of claims 109, wherein the promoter and second promoter are the same.

112. The nucleic acid of any of claims 63-111, wherein said nucleic acid comprises a promoter operably linked to the sequence that encodes the Cas9 molecule of (b).

113. A composition comprising the (a) gRNA molecule of any of claims 1-26.

114. The composition of claim 113, further comprising (b) a Cas9 molecule of any of claims 50-62.

115. The composition of any of claims 113-114, further comprising (c) a second gRNA molecule of any of claims 63-85.

116. The composition of any of claims 113-115, further comprising a third gRNA molecule.

117. The composition of claim 116, further comprising a fourth gRNA molecule.

118. A method of altering a cell comprising contacting said cell with:

(a) a gRNA of any of claims 1-26;

(b) a Cas9 molecule of any of claims 50-62; and

optionally, (c) a second gRNA molecule of any of claims 63-85.

119. The method of claim 118, further comprising a third gRNA molecule.

120. The method of claim 119, further comprising a fourth gRNA molecule.

121. The method of claim 118, comprising contacting said cell with (a), (b), and

(c) .

122. The method of any of claims 118-121, wherein said cell is from a subject suffering from HSV-2.

123. The method of any of claims 118-122, wherein said cell is an epithelial cell.

124. The method of any of claims 118-122, wherein said cell is a neuronal cell.

125. The method of claim 118-124, wherein said contacting step is performed in vivo.

126. The method of any of claims 118-125, comprising acquiring knowledge of the sequence of the HSV-2 target position in said cell.

127. The method of claim 126, comprising acquiring knowledge of the sequence of the HSV-2 target position in said cell by sequencing a portion of the UL19, UL30, UL48 or UL54 gene.

128. The method of any of claims 118-127, comprising inducing a HSV-2 target position mutation.

129. The method of any of claims 118-128, wherein the contacting step comprises contacting said cell with a nucleic acid that encodes at least one of (a), (b), and (c).

130. The method of any of claims 118-129, wherein the contacting step comprises contacting the cell with a nucleic acid of any of claims 27-112.

131. The method of any of claims 118-130, wherein the contacting step comprises delivering to said cell said Cas9 molecule of (b) and a nucleic acid which encodes (a) and optionally (c).

132. The method of any of claims 118-131, wherein the contacting step comprises delivering to said cell said Cas9 molecule of (b), said gRNA molecule of (a) and optionally said second gRNA molecule of (c).

133. The method of any of claims 118-132, wherein the contacting step comprises delivering to said cell said gRNA molecule of (a), optionally said second gRNA molecule of (c) and a nucleic acid that encodes the Cas9 molecule of (b).

134. A method of treating a subject, comprising contacting a subject or a cell from said subject with:

(a) a gRNA of any of claims 1-26;

(b) a Cas9 molecule of any of claims 50-62; and

optionally, (c) a second gRNA of any of claims 63-85.

135. The method of claim 134, further comprising contacting the subject or a cell from the subject with a third gRNA molecule.

136. The method of claim 135, further comprising contacting the subject or a cell from the subject with a fourth gRNA molecule.

137. The method of any of claims 134-136, further comprising contacting said subject or a cell from said subject with (a), (b), and (c).

138. The method of any one of claims 134-137, wherein said subject is suffering from HSV-2.

139. The method of any of claims 134-138, wherein said subject would benefit from a mutation at the HSV-2 target position of the UL19, UL30, UL48 or UL54 gene.

140. The method of any of claims 134-138, wherein said subject would benefit from a mutation in one or more of the UL19, UL30, UL48 and UL54 genes.

141. The method of any of claims 134-138, wherein said subject would benefit from a mutation in two or more of the UL19, UL30, UL48 and UL54 genes.

142. The method of any of claims 134-138, wherein said subject would benefit from a mutation in three or more of the UL19, UL30, UL48 and UL54 genes.

143. The method of any of claims 134-138, wherein said subject would benefit from a mutation in each of the UL19, UL30, UL48 and UL54 genes.

144. The method of any of claims 135-143, comprising acquiring knowledge of the sequence of the HSV-2 target position in said subject.

145. The method of claim 144, comprising acquiring knowledge of the sequence of the HSV-2 target position in said subject by sequencing a portion of the UL19, UL30, UL48 or UL54 gene.

146. The method of any of claims 134-145, comprising inducing a HSV-2 target position mutation in the UL19, UL30, UL48 or UL54 gene.

147. The method of any of claims 134-146, wherein said contacting step is

performed in vivo.

148. The method of claim 147, wherein said contacting step comprises intravenous injection.

149. The method of any of claims 134-148, wherein the contacting step comprises contacting said subject or a cell from the subject with a nucleic acid that encodes at least one of (a), (b), and (c).

150. The method of any of claims 134-149, wherein the contacting step comprises contacting said subject or a cell from the subject with a nucleic acid of any of any of claims 27-112.

151. The method of any of claims 134-150, wherein the contacting step comprises delivering to said subject said Cas9 molecule of (b) and a nucleic acid which encodes and (a) and optionally (c).

152. The method of any of claims 134-150, wherein the contacting step comprises delivering to said subject said Cas9 molecule of (b), and said gRNA of (a) and optionally said second gRNA of (c).

153. The method of any of claims 134-150, wherein the contacting step comprises delivering to said subject said gRNA of (a), optionally said second gRNA of (c) and a nucleic acid that encodes the Cas9 molecule of (b).

154. A reaction mixture comprising a gRNA, a nucleic acid, or a composition described herein, and a cell from a subject having HSV-2, or a subject which would benefit from a mutation at a HSV-2 target position of the UL19, UL30, UL48 or UL54 gene.

155. A kit comprising, (a) gRNA molecule of any of claims 1-26, or nucleic acid that encodes said gRNA, and one or more of the following:

(b) a Cas9 molecule of any of claims 50-62;

(c) a second gRNA molecule of any of claims 63-85; and

(d) nucleic acid that encodes one or more of (b) and (c).

156. The kit of claim 155, comprising nucleic acid that encodes one or more of (a), (b) and (c).

157. The kit of claim 155 or 156, further comprising a third gRNA molecule

targeting a UL19, UL30, UL48 or UL54 target position.

158. The kit of claim 157, further comprising a fourth gRNA molecule targeting a

UL19, UL30, UL48 or UL54 target position.

159. A gRNA molecule of any of claims 1-26 for use in treating HSV-2 in a

subject.

160. The gRNA molecule of claim 159, wherein the gRNA molecule is used in combination with (b) a Cas9 molecule of any of claims 50-62.

161. The gRNA molecule of claims 159 or 160, wherein the gRNA molecule is used in combination with (c) a second gRNA molecule of any of claims 63-85.

162. Use of a gRNA molecule of any of claims 1-26 in the manufacture of a

medicament for treating HSV-2 in a subject.

163. The use of claim 162, wherein the medicament further comprises (b) a Cas9 molecule of any of claims 50-62.

164. The use of any of claims 162 or 163, wherein the medicament further

comprises (c) a second gRNA molecule of any of claims 63-85.

. A composition of any of claims 113-117 for use in treating HSV-2 in a subject.