WO2015003449A1

WO2015003449A1 - Optoacoustic-fluorescence dual-mode endoscope

Info

Publication number: WO2015003449A1
Application number: PCT/CN2013/087652
Authority: WO
Inventors: 宋亮; 白晓淞; 龚小竞
Original assignee: 深圳先进技术研究院
Priority date: 2013-07-12
Filing date: 2013-11-22
Publication date: 2015-01-15
Also published as: CN104274149A; CN104274149B

Abstract

Disclosed is an optoacoustic-fluorescence dual-mode endoscope (10) comprising a control system (11), a laser light source (12), a optical system (13), an endoscope tube (14), a scanning system (15), a data acquisition system (16), an image reconstruction system (17) and a display system (18). The control system (11) controls the laser light source (12), the scanning system (15), the data acquisition system (16), the image reconstruction system (17) and the display system (18). The laser system (12), the optical system (13), the scanning system (15) and the endoscope tube (14) are sequentially connected, and the data acquisition system (16), the optical system (13) and the endoscope tube (14) are connected. The data acquisition system (16), the image reconstruction system (17) and the display system (18) are sequentially connected. This dual-mode endoscope (10) realizes integration of two imaging modes by simultaneously leading the excited optoacoustic and the fluorescent light into the endoscope tube (14), and irradiating into a biological tissue, after a focused photoscope has focused, while exciting the optoacoustic signal and the fluorescence excitation signal, and the imaging resolution is greatly improved.

Description

Photoacoustic-fluorescent dual-mode endoscope

Technical field

The invention belongs to the technical field of endoscopes, and in particular relates to a photoacoustic-fluorescent dual-mode endoscope.

Background technique

As a non-invasive or minimally invasive imaging method, endoscope can penetrate into the internal body cavity of the organism and directly observe the internal organs and tissue characteristics. It is widely used in many fields such as biomedicine and clinical diagnosis and treatment, especially cardiovascular and cerebrovascular digestion. Road and interventional diagnosis.

At present, the commonly used endoscopes are mainly ultrasonic and optical. Ultrasound endoscopy uses reflection ultrasound imaging to reflect tissue structural information, but it has low imaging resolution, low soft tissue contrast, and cannot reflect physiological function changes and molecular information, so it cannot effectively reflect biological tissue. Early lesions. The optical endoscope can only image the surface of the internal biological tissue through the CCD, and the tissue below the epidermis can not be observed, which limits the disease diagnosis ability to a certain extent.

Recently, some new optical endoscopes have emerged, such as near-infrared fluorescence endoscopes and photoacoustic endoscopes. Among them, NIR endoscopes use molecular targeting probes to specifically image biomolecules, which have high sensitivity for early diagnosis of diseases, but it cannot reflect the morphology and structural characteristics of biological tissues, and does not have depth resolution. Therefore, it is not possible to provide more informative 3D imaging. Photoacoustic endoscopic imaging using optical absorption contrast, especially optical resolution photoacoustic endoscope, has high contrast and resolution, and can simultaneously image the morphological structure, chemical composition and physiological function information of biological tissues. It has extremely significant significance and clinical value for early diagnosis of cardiovascular diseases and malignant tumors.

Multimodal imaging methods A variety of imaging modalities have been combined to compensate for the deficiencies of a single imaging modality, which has become a development trend. However, the existing multi-modal endoscopic techniques have low resolution of photoacoustic imaging and fluorescence imaging, and it is difficult to effectively distinguish early lesions, or although the imaging resolution can be improved, but the probe photoacoustic imaging module uses transmission. The method of receiving a photoacoustic signal does not enable true endoscopic imaging to be applied to the detection of a living body cavity.

The invention discloses a photoacoustic-fluorescence multi-mode endoscope, which can obtain a three-dimensional image of tissue structure, chemical composition and physiological function characteristic information with higher optical resolution for early diagnosis of diseases, and can simultaneously obtain biological tissues. High sensitivity biospecific molecular information.

Summary of the invention The invention provides a photoacoustic-fluorescence dual-mode endoscope, which aims to solve the technical problem that the existing endoscope has low image resolution and limited use occasions.

The technical solution provided by the present invention is: a photoacoustic-fluorescence dual-mode endoscope comprising: a control system, a laser light source, an optical path system, an endoscope catheter, a scanning system, a data acquisition system, an image reconstruction system, and a display system, The control system controls the laser light source, the scanning system, the data acquisition system, the image reconstruction system, and the display system, the laser light source, the optical path system, the scanning system, and the inner The speculum catheters are sequentially connected, the data acquisition system is respectively connected to the optical path system and the endoscope catheter, and the data acquisition system is sequentially connected to the image reconstruction system and the display system, the endoscope An endoscope probe is disposed at the end of the mirror catheter, and the endoscope probe is provided with an optical component and a photoacoustic receiver, wherein the laser light emitted by the laser light source enters the endoscope through the optical path system and the scanning system a mirror catheter, after being focused by the optical component, is incident on the biological tissue and excites a photoacoustic signal and a fluorescent signal, the optical acoustic connection Collecting and converting the photoacoustic signal into a photoacoustic electrical signal, the optical component collecting the fluorescent signal and transmitting to the optical path system, the optical path system converting the fluorescent signal into a fluorescent electrical signal, The data acquisition system receives and stores the photoacoustic electrical signal and the fluorescent electrical signal, and the image reconstruction system receives the photoacoustic electrical signal and the fluorescent electrical signal transmitted by the data acquisition system and respectively respectively Converted into a photoacoustic image signal and a fluorescent image signal, the display system receives the photoacoustic image signal and the fluorescent image signal transmitted by the image reconstruction system and performs photoacoustic image and fluorescent image display of the biological tissue.

The technical solution of the present invention has the following advantages or beneficial effects: The photoacoustic-fluorescent dual-mode endoscope provided by the present invention simultaneously introduces photoacoustic and fluorescent excitation light into an endoscope catheter, and is focused and focused by a focusing mirror. By reaching the biological tissue and simultaneously exciting the photoacoustic signal and the fluorescent signal, the integration of the two imaging modes is realized, and the imaging resolution is greatly improved.

DRAWINGS

1 is a structural view of a photoacoustic-fluorescent dual-mode endoscope according to an embodiment of the present invention;

2 is a structural view of an optical path system in the photoacoustic-fluorescent dual-mode endoscope shown in FIG. 1;

3 is a structural view of an endoscope catheter in the photoacoustic-fluorescence dual-mode endoscope shown in FIG. 1;

4 is another structural view of the endoscope catheter in the photoacoustic-fluorescence dual-mode endoscope shown in FIG. 1; FIG. 5 is another structure of the optical path system in the photoacoustic-fluorescence dual-mode endoscope shown in FIG. Figure 6 is a structural view of a scanning system in the photoacoustic-fluorescence dual-mode endoscope shown in Figure 1;

Fig. 7 is a third structural view of the endoscope catheter in the photoacoustic-fluorescence dual-mode endoscope shown in Fig. 1. detailed description

The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to Fig. 1, Fig. 1 is a structural view showing a photoacoustic-fluorescent dual-mode endoscope 10 according to an embodiment of the present invention.

In the present embodiment, the photoacoustic-fluorescence dual-mode endoscope 10 includes: a control system 11, a laser light source 12, an optical path system 13, an endoscope catheter 14, a scanning system 15, a data acquisition system 16, an image reconstruction system 17, and a display system 18, the control system 11 controlling the laser light source 12, the scanning system 15, the data acquisition system 16, the image reconstruction system 17, and the display system 18, the control system 11 and the The laser light source 12, the scanning system 15, the data acquisition system 16, the image reconstruction system 17, and the display system 18 are connected, respectively.

In the present embodiment, the laser light source 12, the optical path system 13, the scanning system 15, and the endoscope catheter 14 are sequentially connected, the data acquisition system 16 and the optical path system 13 and the The endoscope catheters 14 are connected, and the data acquisition system 16 is sequentially coupled to the image reconstruction system 17 and display system 18.

In this embodiment, the laser light source 12 emits a laser having a wavelength range of 400-2500 nm, the laser light source 12 includes a photoacoustic light source and a fluorescent light source, and the photoacoustic light source is a pulsed laser source or an amplitude modulated continuous laser source. The fluorescent light source is a photoacoustic light source or a light source capable of exciting fluorescence.

In the present embodiment, the laser light emitted by the laser light source 12 enters the endoscope catheter 14 through the optical path system 13 and the scanning system 15, and an endoscope probe is provided at the end of the endoscope catheter 14 ( 1 is not shown), the endoscope probe is provided with an optical component and a photoacoustic receiver, the optical component focuses the laser and irradiates the biological tissue and excites the photoacoustic signal and the fluorescent signal, and the photoacoustic receiver collects The photoacoustic signal is converted to a photoacoustic electrical signal, and the optical component collects the fluorescent signal and transmits it to the optical path system 13 via a scanning system 15, which converts the fluorescent signal into The fluorescent electrical signal, the data acquisition system 16 receives and stores the photoacoustic electrical signal and the fluorescent electrical signal, and the image reconstruction system 17 receives the optical acoustic electrical signal transmitted by the data acquisition system 16 and The fluorescent electrical signals are converted into photoacoustic image signals and fluorescence, respectively The image signal, the display system 18 receives the photoacoustic image signal and the fluorescent image signal transmitted by the image reconstruction system 17 for photoacoustic image and fluorescent image display of biological tissue.

Please refer to FIG. 2. FIG. 2 is a structural diagram of the optical path system 13 in the photoacoustic-fluorescent dual-mode endoscope 10 shown in FIG.

In this embodiment, the optical path system 13 includes a first dichroic mirror 1301, a second dichroic mirror 1302, a fiber coupler 1303, a fiber splitter 1304, a photodiode 1305, and an opto-slip ring. 1306, the photodiode 1305 and the photo-slip ring 1306 are disposed in parallel and are connected to the fiber splitter 1304.

In this embodiment, the first dichroic mirror 1301 coaxially directs the photoacoustic light source and the laser light emitted by the fluorescent light source in the laser light source 12 toward the second dichroic mirror 1302, the second The dichroic mirror 1302 transmits the laser light emitted by the laser light source 12 and reflects the excited fluorescent signal, and the fiber coupler 1303 couples the laser light emitted by the laser light source 12 and the reflected excitation fluorescent signal together and splits through the optical fiber. The device 1304 is split into two beams, one of which enters the photodiode 1305 to generate a reference signal, and the other of which b is sequentially emitted to the biological tissue via the photo-slip ring 1306 and the endoscope catheter 14 for imaging.

In the present embodiment, the opto-slip ring 1306 includes a stator and a rotor, the stator is coupled to the fiber splitter 1304, and the rotor is coupled to the endoscope catheter 14.

In this embodiment, the photoelectric slip ring 1306 is composed of an optical fiber slip ring and an electric slip ring coaxially. In this embodiment, the optical path system 13 further includes a filter 1308 and a photodetector 1309. The photodetector 1309 detects the fluorescence transmitted by the second dichroic mirror 1302 through the filter 1308. signal.

In the present embodiment, the photodiode 1305 is coupled to the fiber splitter 1304 for detecting changes in laser energy.

Referring to FIG. 3, FIG. 3 is a detailed structural view of the endoscope catheter 14 in the photoacoustic-fluorescent dual-mode endoscope 10 of FIG.

In the present embodiment, the endoscope catheter 14 includes an optical fiber 141, a cable 142, and a protective cover 143, and the optical fiber 141 and the cable 142 are wrapped in the protective cover 143.

In this embodiment, the optical fiber 141 is a single mode fiber or a double-clad fiber, and the double-clad fiber is composed of a single-mode core and a multi-mode cladding. In the present embodiment, the end of the endoscope catheter 14 is provided with an endoscope probe 144, which is composed of an optical focusing component 1440, a probe protection catheter 1441, a packaging material 1443, and an ultrasound detector 1444. The probe protection catheter 1441 includes an optical window and an acoustic window, and the optical focusing component 1440 and the ultrasonic detector 1444 are mounted in the probe protection catheter 1441, and the probe protection catheter 1441 is connected to the protective sleeve 143. .

In the present embodiment, the encapsulating material 1443 is also used to fix the optical fiber 141 and the cable 142 in the probe protection catheter 143.

In the embodiment, the optical focusing component 1440 is a ball lens connected to the end of the optical fiber 141 for turning the laser light 90 degrees and exiting through the optical window, and the ultrasonic detector 1444 is connected to the cable. 142 ultrasonic transducer.

In the present embodiment, the endoscopic probe 144 further includes an angle adjusting member 1445 disposed in the probe protection catheter 143 for adjusting an angle at which the ultrasonic probe 1444 receives an ultrasonic signal.

Referring to FIG. 4, FIG. 4 is another structural view of the endoscope catheter 14 in the photoacoustic-fluorescent dual-mode endoscope 10 of FIG.

In the present embodiment, the structure of the endoscope catheter 14 is substantially the same as that shown in FIG. 3, except that the structure of the endoscope probe 144 is only the same, and the same portions will not be described herein.

In the present embodiment, the endoscopic probe 144 includes a focusing assembly 1440, a probe protection tube ₁₄ 41, an ultrasonic probe 1444, an angle adjustment member 1445, a diameter matching conduit 1446, a thin-walled conduit 1447, and a mirror 1448.

In the present embodiment, the focusing component 1440 is a self-focusing lens, one end is connected to the end of the optical fiber 141, and is sleeved at the end of the optical fiber 141 by the diameter matching conduit 1446, and the mirror is placed at the other end. 1448, used to convert the laser light emitted by the focusing component 1440 into 90 degrees. The diameter matching sleeve 1446, the autofocus lens 1440 and the mirror 1448 are placed in the thin-walled conduit 1447, and the thin-walled conduit 1447 is provided at the laser exiting position of the mirror 1448. Optical window.

Referring to FIG. 5, FIG. 5 is another structural diagram of the optical path system 13 in the photoacoustic-fluorescent dual-mode endoscope 10 of FIG.

In this embodiment, the optical path system 13 includes a first dichroic mirror 1301 disposed in sequence. The beam splitter 1310, the second dichroic mirror 1302, and the microscope objective 1317, wherein the optical path system 13 further includes a photodiode 1311, a filter 1308, and a photodetector 1309, the photodiode 131 1 and the spectroscopic The mirror 1310 is optically coupled.

In the present embodiment, the first dichroic mirror 1301 couples the photoacoustic light source in the laser light source 12 and the laser light emitted from the fluorescent light source together and coaxially, and the beam splitter 1310 reflects the laser light to the portion. The photodiode 1311 generates a reference signal, and the scanning system 15 controls the transmission direction of the laser light transmitted by the beam splitter 1310 and irradiates the laser light to the microscope objective 1317, which focuses and irradiates the laser to the microscope The end surface of the endoscope catheter 14 is irradiated with a laser beam emitted from the endoscope catheter 14 to excite a photoacoustic signal and a fluorescence signal. The photodetector 1309 detects the fluorescent signal transmitted by the second dichroic mirror 1302 through the filter 1308.

Please refer to FIG. 6, FIG. 6 is a structural diagram of the scanning system 15 in the photoacoustic-fluorescent dual-mode endoscope 10 shown in FIG.

In the present embodiment, the scanning system 15 is composed of two mirrors (a mirror 1501 and a mirror 1502), and the two mirrors (the mirror 1501 and the mirror 1502) are in the same manner as the mirror 1501. The mirrors 1502 are oscillated at a small angle in a plane perpendicular to each other such that the spot focused by the microscope objective 1317 sweeps the end face of the fiber bundle of the endoscope catheter 14.

Referring to Fig. 7, Fig. 7 is a third structural view of the endoscope catheter 14 in the photoacoustic-fluorescent dual-mode endoscope 10 of Fig. 1.

In the present embodiment, the endoscope catheter includes a fiber bundle 141, a cable 142, a protective cover 143, a focusing assembly 146, and an ultrasonic probe 147.

In the present embodiment, the ultrasonic probe 147 is a hollow ultrasonic transducer, and the focusing assembly 146 is disposed in a hollow portion of the ultrasonic transducer. In the embodiment, the focusing component 146 is a self-focusing lens, the fiber bundle 141 is coaxially connected to the focusing component 146, and the fiber bundle 141 and the focusing component 146 are encapsulated in the protective sleeve 143. The spot emitted by the fiber bundle 141 is focused by the focusing component 146 and then irradiated to the biological tissue to excite the photoacoustic signal and the fluorescent signal, and the ultrasonic detector 147 detects the photoacoustic signal and converts it into a photoacoustic electrical signal. The fiber bundle 141 collects a fluorescent signal and transmits it to the photodetector 1309 for conversion into a fluorescent electrical signal.

In the present embodiment, the fiber bundle 141 is composed of a plurality of single-mode fibers, and spots irradiated to different positions of the end faces of the fiber bundles 141 are transmitted to the endoscope catheter 14 through different single-mode fibers. The spots emitted by the different single-mode fibers in the fiber bundle 141 are irradiated to different positions of the biological tissue, and the three-dimensional structure and functional information images of the tissue are obtained by photoacoustic scanning, and the two-dimensional molecular fluorescence of the biological tissue is obtained by fluorescence imaging. image.

The photoacoustic-fluorescent dual-mode endoscope 10 provided in the embodiment of the present invention introduces photoacoustic and fluorescent excitation light into the endoscope catheter 14 at the same time, and focuses the lens through the endoscope probe 144 to illuminate the living body. The tissue, simultaneously exciting the photoacoustic signal and the fluorescent signal, realizes the integration of the two imaging modes, and the imaging resolution is greatly improved.

The above is only the preferred embodiment of the present invention, and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and scope of the present invention should be included in the present invention. Within the scope of protection.

Claims

Rights request

1. A photoacoustic-fluorescence dual-mode endoscope, characterized in that it includes: a control system, a laser light source, an optical path system, an endoscope catheter, a scanning system, a data acquisition system, an image reconstruction system and a display system, as described The control system controls the laser light source, the scanning system, the data acquisition system, the image reconstruction system and the display system. The laser light source, the optical path system, the scanning system and the endoscope The conduits are connected in sequence, the data acquisition system is connected to the optical path system and the endoscopic catheter respectively, and the data acquisition system is connected to the image reconstruction system and the display system in sequence, and the endoscopic catheter An endoscope probe is provided at the end, and the endoscope probe is provided with an optical component and a photoacoustic receiver, wherein the laser emitted by the laser light source enters the endoscopic catheter through the optical path system and the scanning system. , after being focused by the optical component, it irradiates the biological tissue and excites photoacoustic signals and fluorescence signals. The photoacoustic receiver collects the photoacoustic signals and converts them into photoacoustic electrical signals. The optical component collects the photoacoustic signals. The fluorescent signal is transmitted to the optical path system. The optical path system converts the fluorescent signal into a fluorescent electrical signal. The data acquisition system receives and stores the photoacoustic electrical signal and the fluorescent electrical signal. The image reconstruction system receives the photoacoustic electrical signal and the fluorescent electrical signal sent by the data acquisition system and converts them into a photoacoustic image signal and a fluorescent image signal respectively, and the display system receives the photoacoustic electrical signal and the fluorescent electrical signal sent by the image reconstruction system. The photoacoustic image signal and the fluorescent image signal are used to display the photoacoustic image and the fluorescent image of the biological tissue.

2. The photoacoustic-fluorescence dual-mode endoscope according to claim 1, characterized in that the laser wavelength range emitted by the laser light source is 400~2500nm, and the laser light source includes a photoacoustic light source and a fluorescent light source, so The photoacoustic light source is a pulse laser light source or an amplitude-modulated continuous laser light source, and the fluorescent light source is a photoacoustic light source or a light source capable of exciting fluorescence.

3. The photoacoustic-fluorescence dual-mode endoscope according to claim 2, wherein the optical path system includes a first dichroic mirror, a second dichroic mirror, a fiber optic coupler, and a first dichroic mirror arranged in sequence. An optical fiber beam splitter, a photoelectric slip ring and a photodiode. The photodiode and the photoelectric slip ring are arranged in parallel and are connected to the optical fiber beam splitter. The laser light emitted by the photoacoustic light source and the fluorescent light source passes through the third A dichroic mirror emits coaxially, the laser light emitted by the laser light source and the fluorescence signal excited by reflection are transmitted through the second dichroic mirror, and the photodiode is connected to the optical fiber beam splitter for detecting the laser light. Energy changes, the optical fiber coupler couples the laser light emitted by the photoacoustic light source and the fluorescent light source together and passes The optical fiber beam splitter divides the laser beam into two beams, one of which enters the photodiode to generate a reference signal, and the other laser beam is sequentially emitted to the biological tissue through the photoelectric slip ring and the endoscope catheter for imaging.

4. The photoacoustic-fluorescence dual-mode endoscope according to claim 3, characterized in that the optical path system further includes a filter and a photodetector, and the photodetector is used to detect from the second second Direct the fluorescence signal through the filter into the mirror.

5. The photoacoustic-fluorescence dual-mode endoscope according to claim 3, wherein the photoelectric slip ring includes a stator and a rotor, the stator is connected to the optical fiber beam splitter, and the rotor is connected to the optical fiber beam splitter. The endoscopic catheter is connected.

6. The photoacoustic-fluorescence dual-mode endoscope according to claim 1, wherein the endoscope catheter includes an optical fiber, a cable and a protective sheath, and the optical fiber and the cable are wrapped in the protective sheath. Wherein, the optical fiber is a single-mode optical fiber or a double-clad optical fiber, and the double-clad optical fiber is composed of a single-mode fiber core and a multi-mode cladding.

7. The photoacoustic-fluorescence dual-mode endoscope according to claim 6, wherein the endoscope probe is composed of an optical focusing component, a probe protection conduit, a packaging material and an ultrasonic detector, and the probe protection The conduit includes an optical window and an acoustic window. The optical focusing component and the ultrasonic detector are installed in the probe protection conduit. The probe protection conduit is connected to the protective sheath. The packaging material connects the optical fiber and the The cable is fixed in the probe protective conduit.

8. The photoacoustic-fluorescence dual-mode endoscope according to claim 7, characterized in that the optical focusing component is a ball lens connected to the end of the optical fiber, used to turn the laser 90 degrees and pass through the The optical window emerges, and the ultrasonic detector is an ultrasonic transducer connected to the cable. The endoscope probe is also provided with an ultrasonic transducer fixed in the probe protection tube for adjusting the ultrasonic transducer to receive ultrasonic waves. Signal angle adjustment piece.

9. The photoacoustic-fluorescence dual-mode endoscope according to claim 6, wherein the endoscope probe includes: a focusing component, an angle adjustment member, a diameter matching conduit, a thin-walled conduit and a reflector, so The focusing component is a self-focusing lens, one end of which is connected to the end of the optical fiber, and is sleeved on the end of the optical fiber using the diameter matching conduit, and the other end is placed with the reflector for emitting the focusing component. The laser turns 90 degrees, wherein the diameter matching sleeve, the focusing component and the reflector are placed in a thin-walled conduit, and the thin-walled conduit is provided with an optical window at the laser exit position of the reflector. mouth.

10. The photoacoustic-fluorescence dual-mode endoscope according to claim 1, characterized in that the optical path system includes a first dichroic mirror, a beam splitter, a second dichroic mirror and a microscopic objective lens arranged in sequence , wherein the optical path system also includes a photodiode, a filter and a photodetector, the laser light emitted by the laser light source is emitted coaxially through the first dichroic mirror, and the dichroic mirror reflects a part of the laser light. The photodiode generates a reference signal, the scanning system controls the transmission direction of the laser light transmitted by the spectroscope and causes the laser light to be irradiated to the microscopic objective lens, and the microscopic objective lens focuses the laser light and irradiates it to the endoscope. The end face of the endoscope catheter, the laser emitted from the endoscope catheter excites photoacoustic signals and fluorescence signals after irradiating the biological tissue, and the photodetector detects the light transmitted by the second dichroic mirror through the filter. Fluorescent signal.

11. The photoacoustic-fluorescence dual-mode endoscope according to claim 10, characterized in that the scanning system is composed of two mirrors, and the two mirrors swing in a plane perpendicular to them.

12. The photoacoustic-fluorescence dual-mode endoscope according to claim 11, wherein the endoscope catheter includes a gathering assembly, an optical fiber bundle, an ultrasonic detector and a protective sheath, and the ultrasonic detector is hollow The ultrasonic transducer, the focusing component is placed in the hollow part of the ultrasonic transducer, the focusing component is a self-focusing lens, the optical fiber bundle is coaxially connected to the focusing component, the optical fiber bundle and the The focusing component is encapsulated in the protective sheath, wherein the light spot emitted from the optical fiber bundle is focused by the focusing component and then irradiated to the biological tissue to excite photoacoustic signals and fluorescence signals, and the ultrasonic detector detects the photoacoustic signals and It is converted into a photoacoustic electrical signal, and the optical fiber bundle collects the fluorescent signal and transmits it to the photodetector to be converted into a fluorescent electrical signal.