WO2014085774A1 - Pollen compositions and methods for distribution on flowering plants - Google Patents
Pollen compositions and methods for distribution on flowering plants Download PDFInfo
- Publication number
- WO2014085774A1 WO2014085774A1 PCT/US2013/072500 US2013072500W WO2014085774A1 WO 2014085774 A1 WO2014085774 A1 WO 2014085774A1 US 2013072500 W US2013072500 W US 2013072500W WO 2014085774 A1 WO2014085774 A1 WO 2014085774A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- viable pollen
- composition
- pollen
- viable
- flowering plant
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/025—Discharge apparatus, e.g. electrostatic spray guns
- B05B5/03—Discharge apparatus, e.g. electrostatic spray guns characterised by the use of gas, e.g. electrostatically assisted pneumatic spraying
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
- B05B7/02—Spray pistols; Apparatus for discharge
- B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
- B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
- B05B7/14—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas designed for spraying particulate materials
- B05B7/1404—Arrangements for supplying particulate material
Definitions
- the composition comprises a plurality of viable pollen grains; and a combination of at least two water miscible carriers selected from the group consisting of: propylene glycol, glycerol, ethylene glycol, 1,3-butanediol, 1,4-butanediol, and ethyl acetate; or at least one water miscible carrier in an amount of at least 40% of the pollen composition by weight, wherein the at least one water miscible carrier is selected from the group consisting of: propylene glycol, glycerol, ethylene glycol, 1,3-butanediol, 1,4-butanediol, and ethyl acetate.
- the composition comprises a plurality of viable pollen grains; and at least one water miscible carrier selected from the group consisting of: propylene glycol and ethylene glycol.
- Figure 4 depicts the flow rate (mL/min) at the various pump settings for application of the pollen in the slurry mixture during the field trials in the almond grove near Madera, California.
- the at least two water miscible carriers is selected from the group consisting of: propylene glycol and glycerol; glycerol and ethylene glycol; propylene glycol and ethylene glycol; ethyl acetate and glycerol; ethyl acetate and propylene glycol; and ethyl acetate and ethylene glycol.
- TABLE 1 provides a few preferred formulations for solvent blend based on weight % of each ingredient.
- the propylene glycol, glycerol, ethyl acetate, and/or ethylene glycol in the formulation may be at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, or at least 90% of the pollen composition by weight.
- the plurality of pollen grains in the formulation may be about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, or about 50% of the pollen composition by weight.
- the plurality of viable pollen grains is generally added to the formulation at between 1% and 40% of the composition by weight, for example, between 5% and 25%, or more specifically, 7.5% and 20%.
- 5% viable pollen is added to the solvent blend to make the viable pollen composition, there would be 69.5% propylene glycol, 25.65% glycerol and 5% viable pollen based on the weight of the composition.
- the viable pollen compositions include viable pollen grains and at least one water miscible carrier in an amount of at least 20%, 30%, 40%, 50%, 60%, 70%, 75%, or 80% of the pollen composition by weight, wherein the at least one water miscible carrier selected from the group consisting of: propylene glycol, glycerol, ethylene glycol, 1,3-butanediol, and 1,4-butanediol and ethyl acetate, for example, a viable composition comprising propylene glycol in an amount that is at least 35% of the composition by weight.
- the at least one water miscible carrier selected from the group consisting of: propylene glycol, glycerol, ethylene glycol, 1,3-butanediol, and 1,4-butanediol and ethyl acetate, for example, a viable composition comprising propylene glycol in an amount that is at least 35% of the composition by weight.
- the viable pollen composition typically comprises less than 10% water by weight of the composition, e.g., less than 5%, 3%, 1%, or 0.5%, before preparation for distribution and use for pollination of flowing plants.
- An aqueous solution is typically added to the viable pollen composition within 5 seconds, 10 seconds, 5 minutes, 30 minutes, 45 minutes, or an hour of preparation and the mechanical distribution of the viable pollen on the flowing plant.
- the amount of aqueous solution (e.g., water) added to the viable pollen composition in preparation for distribution is generally in amount between, 99.9% and 60%; more specifically more than 75%, 80%, 90%, 95%, or 99%.
- the plurality of viable pollen grains is preferably from an eudicot, e.g., an asterid or rosid.
- pollen grain suitable for use in the compositions include: almond; cherry; pear; apple; pistachio; plum; peach; apricot, avocado; blueberry; melon; cucumber; cotton; coffee; asparagus; onion; broccoli; alfalfa; soy; celery; tangerine, lemon, strawberry, quince, blackberry, and raspberry pollen.
- composition of the present invention may further contain sugars, potassium, calcium, boron, and nitrates. These additives may promote pollen tube growth after pollen distribution on flowering plants.
- Bee attractants may also be included.
- Known bee attractants include pheromones and essential plant oils.
- a "pheromone" is a natural or synthetic chemical substance that triggers a response in members of a species.
- a pheromone that can be used in the present invention is the Nasonov (alternatively, Nasanov) pheromone, which is released by worker bees to orient returning forager bees back to the colony.
- Nasonov includes nerol, (E,E)-farnesol, geraniol, nerolic acid, citral and geranic acid.
- Bees use Nasonov to find the entrance to their colony or hive, and they release it on flowers so other bees know which flowers have nectar.
- Synthetic versions of Nasonov may contain any one of the chemical compounds present in natural Nasonov or any combination of these chemical compounds.
- one synthetic version of Nasonov pheromone consists of citral and geraniol in a 2: 1 ratio.
- Essential oils producing fragrances found in highly scented flowers may also be used in the composition of the present invention. Chemoreceptors in their antennae cause bees to seek out these fragrances.
- One essential oil that may be used is essential oil of anise. Honeybees can identify the fragrance from a few drops of essential oil of anise from a considerable distance.
- the composition of the present invention may contain a preservative to prevent the growth of microorganisms.
- the preventions of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, sorbic acid, and the like.
- Antioxidants may also be added to the pollen suspension to preserve the pollen from oxidative damage during storage. Suitable antioxidants include, for example, ascorbic acid, tocopherol, sulfites, metabisulfites such as potassium metabisulfite, butylhydroxytoluene, and butylhydroxyanisole.
- the invention also provides methods of dispensing viable pollen on a flowering plant.
- the disclosed electrostatic pollination application process may be used on any bee-pollinated plant.
- the aqueous solution is added to the viable pollen composition within 5 seconds, 10 seconds, 5 minutes, 10 minutes, 30 minutes, 45 minutes or an hour of spraying the flowing plant with the viable pollen composition.
- a mixing tank can be used to mix the water and viable pollen composition prior to distribution. The length of time that the pollen is in the aqueous mixture affects viability of the pollen.
- the invention further comprises mixing the viable pollen composition with an aqueous solution from a second container using a mixing valve and/or a mixing tank prior to propelling at least a portion of the viable pollen composition on the flowering plant.
- the aqueous solution is mixed with the viable pollen composition within 5 seconds, 10 seconds, 5 minutes, 15 minutes, 30 minutes, 45 minutes or an hour of dispersing the viable pollen aqueous mixture on a flowing plant.
- the viable pollen mixture is mechanically dispersed using an electrostatic sprayer.
- the dispensing nozzle preferably used in mechanical distribution of the viable pollen mixture forms viable pollen droplets upon spraying or propelling the viable pollen composition onto the flowering plant.
- the ratio of the viable pollen droplet volume compared to the volume of the viable pollen grain is less than 1.5: 1, less than 2.0: 1, less than 2.5: 1, less than 3.0: 1, less than 3.5: 1, less than 4.0: 1, less than 4.5: 1, less than 5.5: 1, less than 6.0: 1, less than 6.5: 1, less than 7.0: 1, less than 7.5: 1, less than 8.0: 1, less than 8.5: 1, less than 9.0: 1, less than 9.5: 1, or less than 10.0: 1.
- the ratio of the viable pollen droplet volume compared to the volume of the viable pollen grain is less than 3.0: 1.
- the methods of the invention may further comprise adding a bee attractant to the pollen suspension and an electrostatic sprayer is used to spray at least a portion of the spray volume on a flowering plant.
- the method of the present invention may further comprise adding a bee attractant to the pollen suspension.
- the resulting droplets may then be applied to flowering plants and bees allowed to contact the flowering plants to increase the efficiency of pollination.
- Small water droplets containing sugars and/or pollen can be extremely stimulating to bee activity. Spraying with this mixture when flowers are mature may lead to increased pollination through increased bee activity.
- the methods of the invention generally are directed to dispensing viable pollen on a flowering plant that is a eudicot, for example, an asterid or rosid and more specifically a plant from the Rosaceae family.
- flowering plants suitable for use with the methods described herein include: almond; cherry; pear; apple; pistachio; plum; peach; apricot, avocado; blueberry; melon; cucumber; cotton; coffee; asparagus; onion; broccoli; alfalfa; soy; celery; tangerine, lemon, strawberry, quince, blackberry, and raspberry pollen.
- the plurality of viable pollen grains in the viable pollen composition is chosen based on what flowering plant the viable pollen mixture will be dispensed on. For example, when the plurality of viable pollen grains in the viable pollen mixture are almond or cherry, the viable pollen mixture will be dispersed on an almond or cherry plant respectively.
- disbursement amounts of the viable pollen composition on the flowering plant in grams per acre is shown below in Table 2 - disbursement preferable occurs soon after mixing with water to preserve viability.
- the mixing tank is used to allow the aqueous solution to be mixed with the viable pollen composition for at least 5 seconds, 5 minutes, 15 minutes, 30 minutes, 45 minutes or 55 minutes.
- a mixing value (3) can be used in conjunction with a mixing tank for delay in of disbursement of the viable pollen mixture to provide certain benefits of bringing the viable pollen in contact with water for a short time, without compromising the viability of the pollen.
- Two dominant methods of producing very small spray droplets are by having a high liquid pressure drop through the nozzle or by using a high velocity air stream going through the nozzle and removing liquid from an orifice.
- the fine spray leaving the nozzle can be charged by ions created in the nozzle by a potential difference induction or corona discharge.
- the droplets are as small as practical while still holding the pollen.
- the droplet weight to charge density is a ratio that may impact how quickly the droplet will attach to the plant being pollinated.
- the diameter of the droplet to the diameter of the pollen grain is about 1.5: 1, about 2.0: 1, about 2.5: 1, about 3.0: 1, about 3.5: 1, about 4.0: 1, about 4.5: 1, or about 5.0: 1.
- Figure 2 shows a droplet (7) and a pollen grain (8) at the preferred ratio are such that the droplet is small as possible and still able to transmit the pollen grain.
- One example is about 3.0: 1 for the diameter of the droplet to the diameter of the pollen grain.
- the volume of the droplet to the volume of the pollen grain is about 1.5: 1, about 2.0: 1, about 2.5: 1, about 3.0: 1, about 3.5: 1, about 4.0: 1, about 4.5: 1, about 5.5: 1, about 6.0: 1, about 6.5: 1, about 7.0: 1, about 7.5: 1, about 8.0: 1, about 8.5: 1, about 9.0: 1, about 9.5: 1, or about 10.0: 1.
- the single-fluid nozzle is a plain orifice nozzle.
- the pressure drop applied may be high (e.g., at least about 25 bar) so that the material is finely atomized.
- the single-fluid nozzle is a shaped orifice nozzle.
- the shaped orifice may use a hemispherical shaped inlet and a "V" notched outlet to cause the flow to spread out on the axis of the V notch.
- the single-fluid nozzle may also be a surface impingement nozzle, which causes a stream of liquid to impinge on a surface resulting in a sheet of liquid that breaks up into drops.
- the impingement surface may be formed in a spiral to yield a spiral shaped sheet approximating a full cone spray pattern or a hollow- cone spray pattern.
- the spiral design generally may produce a smaller drop size than the pressure swirl type nozzle design, for a given pressure and flow rate. This design is clog resistant due to the large free passage.
- the single-fluid nozzle may be a pressure-swirl spray nozzle.
- the stationary core of a pressure-swirl spray nozzle induces a rotary fluid motion, which causes the swirling of the fluid in the swirl chamber.
- a film is discharged from the perimeter of the outlet orifice producing a characteristic hollow cone spray pattern.
- Air or other surrounding gas is drawn inside the swirl chamber to form an air core within the swirling liquid.
- Many configurations of fluid inlets may be used to produce this hollow cone pattern.
- the single-fluid nozzle may be a spill-return pressure-swirl single-fluid nozzle. This nozzle is one variety of pressure swirl nozzle that includes a controlled return of fluid from the swirl chamber to the feed system that allows the nozzle pressure drop to remain high while allowing a wide range of operating rates.
- the single-fluid nozzle is a solid cone single-fluid nozzle.
- the swirling liquid motion is induced with a vane structure, but the discharge flow fills the entire outlet orifice.
- a full cone nozzle will produce a larger drop size than a hollow cone nozzle.
- the spray nozzle may be a two-fluid nozzle.
- Two-fluid nozzles atomize a liquid by causing the interaction of an atomizing gas with the liquid. Compressed air is most often used as the atomizing gas, but sometimes steam or other gases are used.
- the many varied designs of two-fluid nozzles can be grouped into internal mix or external mix depending on the mixing point of the gas and liquid streams relative to the nozzle face.
- the invention includes a two-fluid nozzle that is an internal mix two-fluid nozzle where fluids make contact inside the nozzle. Shearing between high velocity gas and low velocity liquid may disintegrate the liquid stream into droplets, producing a high velocity spray.
- the internal mix nozzle may use less atomizing gas than an external mix atomizer and is better suited to higher viscosity streams.
- the invention includes a two-fluid nozzle that is an external- mix two-fluid nozzle.
- a two-fluid nozzle that is an external- mix two-fluid nozzle.
- the fluids make contact outside the nozzle.
- External-mix two-fluid nozzles may require more atomizing air and a higher atomizing air pressure drop because the mixing and atomization of liquid takes place outside the nozzle.
- the spray nozzle is a compound nozzle.
- a compound nozzle is a type of nozzle in which several individual single-fluid nozzles or two- fluid nozzles are incorporated into one nozzle body. Compound nozzles allow for design control of drop size and spray coverage angle.
- the spray nozzle may produce electrostatic charging of fluid as the fluid leaves the spray nozzle.
- the viable pollen compositions described herein are well suited for use in an electrostatic sprayer. An electric potential difference may be created in the nozzle resulting in a corona discharge and ionization of the spray and droplets leaving the nozzle. Electrostatic charging of sprays is very useful for high transfer efficiency. The charging is typically at high voltage (e.g., 1.5 KV to about 20 kV to about 40 kV) but low current.
- the invention may include a spray nozzle with rotary atomizers. Rotary atomizers use a high speed rotating disk, cup or wheel to discharge liquid at high speed to the perimeter, forming a hollow cone spray.
- the rotational speed controls the drop size.
- the invention may also include a spray nozzle with ultrasonic atomizers, which utilize high frequency (e.g., about 20 kHz to about 50 kHz) vibration to produce narrow drop-size distribution and low velocity spray from a liquid.
- the vibration of a piezoelectric crystal causes capillary waves on the nozzle surface liquid film.
- a mixing paddle is used in the system to ensure the pollen grains remain suspended in the slurry.
- the mixing paddle along with the similar densities between water miscible carriers and the pollen grains prevent the pollen grains from settling out of the slurry mixture or rising to the top of the mixture.
- the slurry is mixed with the water in the system before being sprayed and is applied to the plants within a matter of seconds after mixing with water. For example, there may be a delay of about 5 seconds, about 10 seconds, about 20 seconds, about 30 seconds, about 40 seconds, about 50 seconds, or about 60 seconds between the time the slurry is mixed with water and the time that it is applied to plants.
- the test was conducted on almonds Prunus dulcis that are pollinated with the honeybees, Apis mellifera, at 1.75 hives per acre and nine frames of bees per hive.
- the pollen used in the experiment was N+.Pe.PD pollen obtained from Firman Pollen, e.g., Neplus Ultra (50% match Nonpareil, 50% match Monterey); Peerless (100% match Nonpareil, 50% match Monterey); Padre (100% match Nonpareil, 50% match Monterey).
- the sprayer used to distribute the pollen was provided by Electrostatic Spray Systems (ESS). Rows used in the experiment were 2376 feet long and had 140 trees spaced 17 feet apart down the row. Rows were 22 feet apart and one row was 1.200 acres.
- Rows 2-27 were used in the test (see Figure 3). Rows 1, 28, 29, 30 were excluded for being partial rows and on the perimeter.
- the field consists of Non-Pareil almond variety on the even rows and Monterey variety planted on the odd rows. On the Monterey rows every 10th tree was replaced with the Carmel variety of almonds. The field was located near Madera, California.
- Pollen was mixed into a slurry mixture made from a base slurry mixture comprising 100% glycerol, with future slurry mixtures to be tested later comprising 23% w/w glycerol and 77% w/w propylene glycol.
- the final slurry mixture contained between about 10% w/w and 25% w/w pollen grains diluted in the base slurry.
- the slurry mixture was pumped into a water stream that goes to spray nozzles where the liquid is electrostatically charged.
- the amount of water added to the slurry was based on the tractor speed. In the experimental design, tractor speed was really a pseudonym variable for amount of water added. The objective was to minimize water added during spray and to measure to see the effect. For example, a tractor travelling half the speed with same pollen delivery would require twice the volume of water added.
- Air pressurized by a turbo charger blew the electrostatically charged liquid out of the nozzle and to the trees.
- the pollen was attracted electrostatically to the tree branches and especially to the stigma of the flower where pollination was completed.
- the water spray had an additive to decrease osmotic pressure on the pollen.
- Sucrose was added at a 10% level to the water to decrease the osmotic pressure.
- the water spray rate was about 9 gallons per acre.
- the pump had an adjustable piston stroke so that the amount of pollen slurry delivered to the water stream going to the nozzles could be controlled. Due to the high viscosity of the fluid, the maximum flow rate was at less than a setting of 2.0 with 10.0 being the highest setting. The flow rate achieved by the various pump settings is shown in Figure 4. The relatively low flow rate required a reduction in the tractor speed during the test.
- the tractor pulling the spray equipment traveled at 4.3 miles per hour for all conditions except where the pollen was delivered at 20 g per pass. This was the highest delivery rate for the pollen and the tractor had to go down a gear to travel at 2.8 mph to maintain the necessary power take-off (PTO) shaft speed for the correct air pressure.
- PTO power take-off
- the pollen slurry was pumped at the required speed to give the desired delivery of pollen per acre.
- the experiment was set up as a 2 2 full factorial design with a control. There were three replicates for each of the four conditions. The control was limited to one row each of the Monterey and Non-Pareil. After eliminating the outside rows we divided the remaining rows for treatment as shown in TABLE 3. Pollination spray dates were on three consecutive days (for the three spays).
- TABLES 5 and 6 indicate the differences in yield between almond trees treated with 10 grams of pollen per acre and those treated with 40 grams of pollen per acre and those treated with two sprays versus those treated with three sprays, respectively.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201380061982.9A CN104936436A (en) | 2012-11-28 | 2013-11-29 | Pollen compositions and methods for distribution on flowering plants |
MX2015006626A MX2015006626A (en) | 2012-11-28 | 2013-11-29 | Pollen compositions and methods for distribution on flowering plants. |
EP13857954.5A EP2925120A4 (en) | 2012-11-28 | 2013-11-29 | Pollen compositions and methods for distribution on flowering plants |
AU2013351965A AU2013351965A1 (en) | 2012-11-28 | 2013-11-29 | Pollen compositions and methods for distribution on flowering plants |
US14/723,733 US10076091B2 (en) | 2012-11-28 | 2015-05-28 | Pollen compositions and methods for distribution on flowering plants |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261730639P | 2012-11-28 | 2012-11-28 | |
US61/730,639 | 2012-11-28 | ||
US201361883969P | 2013-09-27 | 2013-09-27 | |
US61/883,969 | 2013-09-27 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/723,733 Continuation-In-Part US10076091B2 (en) | 2012-11-28 | 2015-05-28 | Pollen compositions and methods for distribution on flowering plants |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2014085774A1 true WO2014085774A1 (en) | 2014-06-05 |
Family
ID=50828521
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2013/072500 WO2014085774A1 (en) | 2012-11-28 | 2013-11-29 | Pollen compositions and methods for distribution on flowering plants |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP2925120A4 (en) |
CN (1) | CN104936436A (en) |
AU (1) | AU2013351965A1 (en) |
CL (1) | CL2015001426A1 (en) |
MX (1) | MX2015006626A (en) |
WO (1) | WO2014085774A1 (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106258943A (en) * | 2016-09-29 | 2017-01-04 | 浙江大学 | Backpack breeding of hybrid rice pollination machine and method thereof |
US9943049B2 (en) | 2015-08-12 | 2018-04-17 | Dina Safreno | Vision-based pollination system |
CN109220775A (en) * | 2018-09-18 | 2019-01-18 | 吴江市黎里心怡农业科技有限公司 | A kind of application of the sudden and violent powder promotor of Cuiguan pear |
PL423647A1 (en) * | 2017-11-29 | 2019-06-03 | B Droix Spolka Z Ograniczona Odpowiedzialnoscia | Mechanism for rapid mechanical pollination of plants |
CN110583473A (en) * | 2019-10-10 | 2019-12-20 | 陕西汉唐农业标准化研究院有限公司 | Wisdom agricultural is with trunk plant pollination device |
US10577103B2 (en) | 2016-09-08 | 2020-03-03 | Walmart Apollo, Llc | Systems and methods for dispensing an insecticide via unmanned vehicles to defend a crop-containing area against pests |
CN111405845A (en) * | 2017-09-07 | 2020-07-10 | 埃得特农业精密技术有限公司 | System for dry artificial pollination of cultivated trees or shrubs by arbo pollen and method for dry artificial pollination |
US11304355B2 (en) | 2018-05-06 | 2022-04-19 | Weedout Ltd. | Methods and systems for reducing fitness of weed |
WO2022115974A1 (en) * | 2020-12-04 | 2022-06-09 | Tironi Gallardo Nicolas | Procedure and composition for the pollination of the avocado tree persea americana mill |
US11369116B2 (en) | 2016-05-22 | 2022-06-28 | Weedout Ltd. | Compositions, kits and methods for weed control |
CN115443906A (en) * | 2022-10-18 | 2022-12-09 | 西北农林科技大学 | Accurate pollination robot of kiwi fruit based on visual perception and double-flow spraying |
EP4037473A4 (en) * | 2019-10-01 | 2023-05-24 | Monsanto Technology LLC | Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants |
WO2023130002A3 (en) * | 2022-01-03 | 2023-08-03 | Inari Agriculture Technology, Inc. | Pollen-mediated industrial trait delivery in hybrid f2 progeny seed |
US11812735B2 (en) | 2018-05-06 | 2023-11-14 | Weedout Ltd. | Methods of controlling weed of the Amaranth genus |
US11957097B2 (en) | 2018-10-25 | 2024-04-16 | Weedout Ltd. | Methods of inhibiting growth of weeds |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106106126A (en) * | 2016-06-29 | 2016-11-16 | 固镇县华丰蔬菜专业合作社 | A kind of apple tree artificial pollination method |
CN106069727A (en) * | 2016-06-30 | 2016-11-09 | 固镇县华丰农业有限公司 | A kind of artificial pollination method for pear tree |
CN106106128A (en) * | 2016-06-30 | 2016-11-16 | 固镇县华丰农业有限公司 | A kind of artificial pollination method of Fructus actinidiae chinensis |
CN107114232B (en) * | 2017-03-21 | 2023-06-23 | 浙江喜盈天农业开发有限公司 | Pollen solution sprayer |
CN107312741A (en) * | 2017-06-07 | 2017-11-03 | 常州瑞坦商贸有限公司 | A kind of preparation method for red fuji apple pollination pollen suspension |
CN109588305B (en) * | 2018-12-20 | 2021-07-20 | 江苏大学 | Pneumatic slight-elevation greenhouse strawberry pollination robot and implementation method thereof |
CN112535151B (en) * | 2020-12-08 | 2023-04-25 | 吉林省农业科学院 | Liquid composition for attracting bees, solid bee attractant and preparation method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2036706C1 (en) * | 1993-04-29 | 1995-06-09 | Товарищество с ограниченной ответственностью Научно-технический центр "Яха" | Gas-and-liquid generator |
RU2089301C1 (en) * | 1991-02-01 | 1997-09-10 | Рауссел-Юклаф | Method and device for mixing and metering of liquid active ingredient with liquified gaseous pulverizing agent, device ejector and container for liquid concentrate of active ingredient |
US20020121046A1 (en) * | 1988-09-09 | 2002-09-05 | Yamashita Thomas T. | Method and composition for promoting and controlling growth of plants |
CN101743875A (en) * | 2009-12-29 | 2010-06-23 | 新疆生产建设兵团农二师农业科学研究所 | Method for preparing solution of fruit tree pollen and performing pollination |
CN102067808A (en) * | 2009-11-23 | 2011-05-25 | 牛俊武 | Apple pollens capable of being pollinated through spraying liquid and preparation method thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5066594A (en) * | 1989-05-09 | 1991-11-19 | Dna Plant Technology | Method for the manipulation of pollen in plants |
JP5143579B2 (en) * | 2008-01-30 | 2013-02-13 | 株式会社ミズホケミカル | Powder composition for solution pollination |
JP5235164B2 (en) * | 2009-05-29 | 2013-07-10 | エーザイ生科研株式会社 | Pollination agent, aqueous pollen agent, pollen aqueous dispersant and pollination method |
-
2013
- 2013-11-29 MX MX2015006626A patent/MX2015006626A/en unknown
- 2013-11-29 CN CN201380061982.9A patent/CN104936436A/en active Pending
- 2013-11-29 AU AU2013351965A patent/AU2013351965A1/en not_active Abandoned
- 2013-11-29 WO PCT/US2013/072500 patent/WO2014085774A1/en active Application Filing
- 2013-11-29 EP EP13857954.5A patent/EP2925120A4/en not_active Withdrawn
-
2015
- 2015-05-26 CL CL2015001426A patent/CL2015001426A1/en unknown
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020121046A1 (en) * | 1988-09-09 | 2002-09-05 | Yamashita Thomas T. | Method and composition for promoting and controlling growth of plants |
RU2089301C1 (en) * | 1991-02-01 | 1997-09-10 | Рауссел-Юклаф | Method and device for mixing and metering of liquid active ingredient with liquified gaseous pulverizing agent, device ejector and container for liquid concentrate of active ingredient |
RU2036706C1 (en) * | 1993-04-29 | 1995-06-09 | Товарищество с ограниченной ответственностью Научно-технический центр "Яха" | Gas-and-liquid generator |
CN102067808A (en) * | 2009-11-23 | 2011-05-25 | 牛俊武 | Apple pollens capable of being pollinated through spraying liquid and preparation method thereof |
CN101743875A (en) * | 2009-12-29 | 2010-06-23 | 新疆生产建设兵团农二师农业科学研究所 | Method for preparing solution of fruit tree pollen and performing pollination |
Non-Patent Citations (1)
Title |
---|
See also references of EP2925120A4 * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9943049B2 (en) | 2015-08-12 | 2018-04-17 | Dina Safreno | Vision-based pollination system |
US11369116B2 (en) | 2016-05-22 | 2022-06-28 | Weedout Ltd. | Compositions, kits and methods for weed control |
US10577103B2 (en) | 2016-09-08 | 2020-03-03 | Walmart Apollo, Llc | Systems and methods for dispensing an insecticide via unmanned vehicles to defend a crop-containing area against pests |
CN106258943A (en) * | 2016-09-29 | 2017-01-04 | 浙江大学 | Backpack breeding of hybrid rice pollination machine and method thereof |
CN111405845A (en) * | 2017-09-07 | 2020-07-10 | 埃得特农业精密技术有限公司 | System for dry artificial pollination of cultivated trees or shrubs by arbo pollen and method for dry artificial pollination |
EP3678470A4 (en) * | 2017-09-07 | 2020-10-07 | Edete Precision Technologies For Agriculture Ltd. | System for dry artificial pollination of cultivated trees or shrubs by insect-borne pollen and method of doing the same |
PL423647A1 (en) * | 2017-11-29 | 2019-06-03 | B Droix Spolka Z Ograniczona Odpowiedzialnoscia | Mechanism for rapid mechanical pollination of plants |
US11304355B2 (en) | 2018-05-06 | 2022-04-19 | Weedout Ltd. | Methods and systems for reducing fitness of weed |
US11812735B2 (en) | 2018-05-06 | 2023-11-14 | Weedout Ltd. | Methods of controlling weed of the Amaranth genus |
CN109220775A (en) * | 2018-09-18 | 2019-01-18 | 吴江市黎里心怡农业科技有限公司 | A kind of application of the sudden and violent powder promotor of Cuiguan pear |
US11957097B2 (en) | 2018-10-25 | 2024-04-16 | Weedout Ltd. | Methods of inhibiting growth of weeds |
EP4037473A4 (en) * | 2019-10-01 | 2023-05-24 | Monsanto Technology LLC | Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants |
CN110583473A (en) * | 2019-10-10 | 2019-12-20 | 陕西汉唐农业标准化研究院有限公司 | Wisdom agricultural is with trunk plant pollination device |
WO2022115974A1 (en) * | 2020-12-04 | 2022-06-09 | Tironi Gallardo Nicolas | Procedure and composition for the pollination of the avocado tree persea americana mill |
WO2023130002A3 (en) * | 2022-01-03 | 2023-08-03 | Inari Agriculture Technology, Inc. | Pollen-mediated industrial trait delivery in hybrid f2 progeny seed |
WO2023129998A3 (en) * | 2022-01-03 | 2023-08-10 | Inari Agriculture Technology, Inc. | Pollen-mediated feed trait delivery in hybrid f2 progeny seed |
CN115443906A (en) * | 2022-10-18 | 2022-12-09 | 西北农林科技大学 | Accurate pollination robot of kiwi fruit based on visual perception and double-flow spraying |
Also Published As
Publication number | Publication date |
---|---|
EP2925120A4 (en) | 2016-08-24 |
CN104936436A (en) | 2015-09-23 |
EP2925120A1 (en) | 2015-10-07 |
CL2015001426A1 (en) | 2016-04-15 |
AU2013351965A1 (en) | 2015-05-28 |
MX2015006626A (en) | 2016-11-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10076091B2 (en) | Pollen compositions and methods for distribution on flowering plants | |
EP2925120A1 (en) | Pollen compositions and methods for distribution on flowering plants | |
Hamner et al. | The herbicidal action of 2, 4 dichlorophenoxyacetic and 2, 4, 5 trichlorophenoxyacetic acid on bindweed | |
Looney | Improving fruit size, appearance, and other aspects of fruit crop" quality" with plant bioregulating chemicals | |
Doruchowski et al. | Low-drift nozzles vs. standard nozzles for pesticide application in the biological efficacy trials of pesticides in apple pest and disease control | |
DE2749292C2 (en) | ||
CN102770021A (en) | Oil formulations comprising cylcopropene compounds | |
WO2003096806A2 (en) | Vegetal reinforcing agents based on phytohormones | |
JPH08500087A (en) | Novel method and composition for thinning fruits | |
CN109006848B (en) | Nereid bee attractant and method for pollinating momordica grosvenori | |
BE1023957A9 (en) | LIMONENE: FORMULATION AND INSECTICIDE USE | |
CN106719614B (en) | A method of fructus lycii insect pest is cut down using food calling and environmental-friendly insectifuge | |
CN113367061A (en) | Method for improving pollination rate of soybeans | |
EP3091836B1 (en) | Use of a chemical agent for thinning of stone fruit | |
HUE031523T2 (en) | Methods of reducing phytotoxicity of a pesticide | |
Fallahi et al. | Blossom thinning of ‘Law Rome Beauty’apple with hydrogen cyanamide and monocarbamide dihydrogensulfate | |
Helal et al. | Effect of some growth regulators and boron on fruiting and quality of orange | |
RU2677030C2 (en) | Biological active synergetic composition | |
EA029212B1 (en) | Biologically active synergetic composition | |
Masoud et al. | Effects of spraying yeast, algae and fish oil on growth and fruiting of ruby seedless grapevines | |
Riehl et al. | Effect of oil spray application timing on juice quality, yield, and size of Valencia oranges in a southern California orchard | |
Oakford et al. | A comparison of high-and low-volume spray techniques in the thinning of ‘Golden Delicious’ apples | |
Jhade et al. | Foliar application of ethephon (ethrel) for quick defoliation in pomegranate (Punica granatum L.) var. Bhagwa | |
Stewart et al. | Effects on citrus of 2, 4-D used as an amendment to oil sprays | |
Fujisawa et al. | Application of gibberellic acid and CPPU increases berry size and yield of highbush blueberry (Vaccinium corymbosum)'Spartan' |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 13857954 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: MX/A/2015/006626 Country of ref document: MX |
|
ENP | Entry into the national phase |
Ref document number: 2013351965 Country of ref document: AU Date of ref document: 20131129 Kind code of ref document: A |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112015012135 Country of ref document: BR |
|
REEP | Request for entry into the european phase |
Ref document number: 2013857954 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2013857954 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 112015012135 Country of ref document: BR Kind code of ref document: A2 Effective date: 20150526 |