WO2011027301A1 - Algae spirulina platensis preservation by natural honey - Google Patents

Algae spirulina platensis preservation by natural honey Download PDF

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Publication number
WO2011027301A1
WO2011027301A1 PCT/IB2010/053921 IB2010053921W WO2011027301A1 WO 2011027301 A1 WO2011027301 A1 WO 2011027301A1 IB 2010053921 W IB2010053921 W IB 2010053921W WO 2011027301 A1 WO2011027301 A1 WO 2011027301A1
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Prior art keywords
spirulina platensis
biomass
cyanobacterium
honey
dry
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PCT/IB2010/053921
Other languages
French (fr)
Inventor
Vytas RIMKUS
Algimantas Simkus
Original Assignee
Spila, Uab
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Publication date
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Publication of WO2011027301A1 publication Critical patent/WO2011027301A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/748—Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60—Edible seaweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25—Honey; Honey substitutes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives

Definitions

  • Fresh and air-dry biomass of cyanobacterium Spirulina platensis can be preserved with fructose and glucose. These monosaccharides are the main ingredient of bee honey.
  • the dosage of the preparation is 1 - 15 g a day.
  • This invention relates to the field of food supplement production, in particular, to the production of cyanobacterium Spirulina platensis food supplement.
  • Food supplements include vitamins, minerals, acids, enzymes and other vital substances or their complexes. Deficiency of these substances can lead to impaired functioning of organism and development of various diseases. Often these substances are used as preventive measures against disease development or for suppressing disease progression.
  • the search of new supplements has encouraged the analysis of human and other animals' physiology. The result of these efforts - revolutionary supplements which are drastically different from the primary product placed on the market, e. i. the protein concentrate.
  • the present supplements affect the processes of cells as well as genes. It shows the unique properties and effectiveness of these supplements. However, only natural and organic supplements are the most promising ones [1, 2].
  • Cyanobacterium Spirulina platensis is the only living organism that has not changed over the million years of its existence due to its unique biochemical composition. Thoroughly balanced by nature, this algae is a great source of vitamins, minerals and amino-acids. Spirulina platensis is rich in proteins of high biological value. They make up as much as 70 percent of the chemical composition of Spirulina platensis. Spirulina contains high amount of blue pigment phycocyanin which is the only effective natural protection against cancer. This pigment can be found nowhere else in nature. The cyanobacterium has high content of folic acid which is essential for proper hemoglobin formation. In addition, Spirulina contains many vital minerals and micro-elements including ferrum, calcium, sodium, potassium, copper, magnesium, zinc, phosphorus, selenium, vitamins, carotene, nucleic acid, enzymes and other active substances [5].
  • Spirulina platensis has the following unique properties: destroys the majority of viruses; compensates the deficiency of vitamins and minerals; reduces fat levels in the blood, prevents atherosclerosis and coronary diseases; accelerates wound healing; encourages the regeneration of cells; exhibits enzymatic activity; increases the resistance to radiation; normalises metabolic processes, enhances immune system and body resistance; alleviates allergy and intolerance; reduces the toxic effect of carcinogens; removes heavy metals, toxins and radionuclides from the body; possesses curative and preventive properties against body impairments and disorders; increases energy levels and vitality; exhibits prebiotic properties [3, 4, 6].
  • Honey promotes liver health, relieves stress, possesses healing and preventive properties against respiratory, heart and blood vessel diseases, prevents joint, kidney and digestive tract diseases, treats burns, helps battle physical and mental exhaustion, enhances immune system and epidermal skin layer.
  • Honey reduces blood pressure and may be used for the treatment of hypertension even in patients suffering from kidney disease or during pregnancy.
  • Honey is rich in minerals, vitamins, enzymes, phytoncides, hormones, aromatic and bactericidal substances.
  • the algae Spirulina platensis biomass can be well preserved with fructose and glucose.
  • the said carbohydrates constitute the basis of bee honey.
  • the aim of the envisaged invention is to create a safe food supplement which would have positive effect on human physiological processes, enhance activity of the vital body systems and supplement the diet with essential substances.
  • the stated aim is achieved when the human ration involves a food supplement containing natural cyanobacterium Spirulina platensis biomass which has at least 8.0 % of the blue pigment phycocyanin on the dry matter basis, and is preserved with liquid or creamed (partially cristallised) bee honey.
  • the algae are preserved with bee honey at the following ratio of ingredients (% by mass): 0.85 -20 cyanobacterium Spirulina platensis biomass, the rest is liquid or creamed honey.
  • the cyanobacterium Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture.
  • the vacuumed biomass of cyanobacterium Spirulina platensis is poured while mixing into the liquid or creamed honey. The mixing is done for 15 - 30 minutes.
  • the cyanobacterium Spirulina platensis biomass preserved with honey remains active for up to 12 months.
  • the stated aim is achieved when the human ration involves a food supplement containing natural cyanobacterium Spirulina platensis biomass which has at least 8.0 % of the blue pigment phycocyanin on the dry matter basis, and which is dried under active ventilation to an air-dry substance having 9.5 - 14.0 % of moisture and bee honey.
  • the cyanobacterium Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture content.
  • the vacuumed biomass is dried in the drying facility under 36-42 °C temperature.
  • the air-dry cyanobacterium Spirulina platensis biomass remains active for at least 24 months.
  • the algae is additionally preserved with bee honey at the following ratio of ingredients (% by mass): 0.5 -50 of air-dry cyanobacterium Spirulina platensis biomass, the rest is liquid or creamed bee honey.
  • the air-dry biomass of cyanobacterium Spirulina platensis is poured while mixing into the liquid or creamed honey. The mixing is done for 15 - 30 minutes.
  • the cyanobacteriurn Spirulina platensis biomass preserved with honey remains active for up to 36 months.
  • the human ration supplemented with a mixture of Spirulina platensis biomass and bee honey, supplies human body with all essential substances required for the vital physiological processes.
  • the daily allowance is 1— 15 g per person.

Abstract

Biomass of algae Spirulina platesnis both in fresh and dry form can be preserved by glucose and fructose. These monosacharides are the main components of bee honey. Made algae Spirulina platesnis and honey preparation complements the human diet for normal functioning almost all the necessary materials. The dose of preparation is 1 to 15 g per day. The invention claimed relates to food products and/or to food supplements, in particularly, to manufacture of specifically food product and/or food supplement of algae Spirulina platensis.

Description

ALGAE SPIRULINA PLATENSIS PRESERVATION BY NATURAL
HONEY
Fresh and air-dry biomass of cyanobacterium Spirulina platensis can be preserved with fructose and glucose. These monosaccharides are the main ingredient of bee honey. The preparation, containing cyanobaterium Spirulina platensis and bee honey, supplements the human diet with almost all vital substances required for the proper functioning of the organism. The dosage of the preparation is 1 - 15 g a day. This invention relates to the field of food supplement production, in particular, to the production of cyanobacterium Spirulina platensis food supplement.
Food supplements include vitamins, minerals, acids, enzymes and other vital substances or their complexes. Deficiency of these substances can lead to impaired functioning of organism and development of various diseases. Often these substances are used as preventive measures against disease development or for suppressing disease progression. The search of new supplements has encouraged the analysis of human and other animals' physiology. The result of these efforts - revolutionary supplements which are drastically different from the primary product placed on the market, e. i. the protein concentrate. The present supplements affect the processes of cells as well as genes. It shows the unique properties and effectiveness of these supplements. However, only natural and organic supplements are the most promising ones [1, 2].
Cyanobacterium Spirulina platensis is the only living organism that has not changed over the million years of its existence due to its unique biochemical composition. Thoroughly balanced by nature, this algae is a great source of vitamins, minerals and amino-acids. Spirulina platensis is rich in proteins of high biological value. They make up as much as 70 percent of the chemical composition of Spirulina platensis. Spirulina contains high amount of blue pigment phycocyanin which is the only effective natural protection against cancer. This pigment can be found nowhere else in nature. The cyanobacterium has high content of folic acid which is essential for proper hemoglobin formation. In addition, Spirulina contains many vital minerals and micro-elements including ferrum, calcium, sodium, potassium, copper, magnesium, zinc, phosphorus, selenium, vitamins, carotene, nucleic acid, enzymes and other active substances [5].
Studies all across the world have shown that Spirulina platensis has the following unique properties: destroys the majority of viruses; compensates the deficiency of vitamins and minerals; reduces fat levels in the blood, prevents atherosclerosis and coronary diseases; accelerates wound healing; encourages the regeneration of cells; exhibits enzymatic activity; increases the resistance to radiation; normalises metabolic processes, enhances immune system and body resistance; alleviates allergy and intolerance; reduces the toxic effect of carcinogens; removes heavy metals, toxins and radionuclides from the body; possesses curative and preventive properties against body impairments and disorders; increases energy levels and vitality; exhibits prebiotic properties [3, 4, 6].
Bee honey promotes liver health, relieves stress, possesses healing and preventive properties against respiratory, heart and blood vessel diseases, prevents joint, kidney and digestive tract diseases, treats burns, helps battle physical and mental exhaustion, enhances immune system and epidermal skin layer. Honey reduces blood pressure and may be used for the treatment of hypertension even in patients suffering from kidney disease or during pregnancy. Honey is rich in minerals, vitamins, enzymes, phytoncides, hormones, aromatic and bactericidal substances. The algae Spirulina platensis biomass can be well preserved with fructose and glucose. The said carbohydrates constitute the basis of bee honey.
The aim of the envisaged invention is to create a safe food supplement which would have positive effect on human physiological processes, enhance activity of the vital body systems and supplement the diet with essential substances.
The stated aim is achieved when the human ration involves a food supplement containing natural cyanobacterium Spirulina platensis biomass which has at least 8.0 % of the blue pigment phycocyanin on the dry matter basis, and is preserved with liquid or creamed (partially cristallised) bee honey.
The algae are preserved with bee honey at the following ratio of ingredients (% by mass): 0.85 -20 cyanobacterium Spirulina platensis biomass, the rest is liquid or creamed honey. Before preservation, the cyanobacterium Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture. The vacuumed biomass of cyanobacterium Spirulina platensis is poured while mixing into the liquid or creamed honey. The mixing is done for 15 - 30 minutes. The cyanobacterium Spirulina platensis biomass preserved with honey remains active for up to 12 months.
The stated aim is achieved when the human ration involves a food supplement containing natural cyanobacterium Spirulina platensis biomass which has at least 8.0 % of the blue pigment phycocyanin on the dry matter basis, and which is dried under active ventilation to an air-dry substance having 9.5 - 14.0 % of moisture and bee honey. Before drying, the cyanobacterium Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture content. The vacuumed biomass is dried in the drying facility under 36-42 °C temperature. The air-dry cyanobacterium Spirulina platensis biomass remains active for at least 24 months. The algae is additionally preserved with bee honey at the following ratio of ingredients (% by mass): 0.5 -50 of air-dry cyanobacterium Spirulina platensis biomass, the rest is liquid or creamed bee honey. The air-dry biomass of cyanobacterium Spirulina platensis is poured while mixing into the liquid or creamed honey. The mixing is done for 15 - 30 minutes. The cyanobacteriurn Spirulina platensis biomass preserved with honey remains active for up to 36 months.
The human ration, supplemented with a mixture of Spirulina platensis biomass and bee honey, supplies human body with all essential substances required for the vital physiological processes. The daily allowance is 1— 15 g per person.
References:
1. Hamidreza R., Werner S. Great successes in the lowering of pH value in the small intestine of mono gastric animals by using natural feed additives and lowering the macro elements. Tagungsband 4. Boku-Symposium, 2005. P.114-119.
2. Jie Wang, Yin Wang, Zhixu Wang, Lei Li, Jian Qin, Weiqi Lai, Ying Fu, Paolo M Suter, Robert M Russell, Michael A Grusak, Guangwen Tang, Shian Yin. Vitamin A equivalence of spirulina β-carotene in Chinese adults as assessed by using a stable- isotope reference method1,2,3'4. American Journal of Clinical Nutrition, 2009. Vol. 89. No. 6. P. 1776-1783.
3. Patil G.; Chethana S.; Madhusudhan M. C; Raghavarao . S. M. S. Fractionation and purification of the phycobiliproteins from Spirulina platensis. Bioresource Technology, 2008, Vol. 99. P. 7393-7396
4. EropOBa E. A., ΓΜΟΙΠΗΗΟΚΗΗ H. B., 3opHH C. H., Ma30 B. K. H3yneHHe HMMyHOMOflynHpyroiiiHx CBOHCTB ceneHcoaepacaiiiero <|>HKou;HaHHHa. Bonpocw nHTamw, 2006. T. 75. N. 2. C. 19-21.
5. 3apeuKaa E. C, ΓΜΟ-IIHHCKHH H. B., Ma30 B.K., 3opHH C. H., AjieiuKo-0»ceBCKHH K).
Π. Pacnpe^eneHHe acceHirHajibHbix MHKpoaneMeHTOB BO φρβκπΗΑχ GnoMaccw nnrneBOH ΜΗκροΒθΛθροαΐΗ Spirulina platensis. Bonpocw nHTamifl, 2004. T. 73. N. 2. C. 28-31.
6. Ma3o B. K., rMOuiHHCKHH . B., 3njiOBa H. C.
Figure imgf000004_0001
cnnpyjiHHa B nHTaHHH HejiOBeKa. Bonpocw nHTamia, 2004. N. 1. C. 45^53.

Claims

1. The food supplement containing cyanobacterium Spirulina platensis which has at least 8.0% of the blue pigment phycocyanin on the dry matter basis, characterized in that the cyanobacterium is in the form of a fresh Spirulina platensis biomass, and it additionally contains bee honey at the following ratio of ingredients (% by mass): 0.85 - 20 of cyanobacterium Spirulina platensis, the rest is liquid or creamed honey.
2. The production method of the food supplement according to claim 1, characterized in that the Spirulina platensis biomass before preservation is washed with drinking water and vacuumed to 70 - 75 % moisture; the vacuumed cyanobacterium Spirulina platensis biomass is poured while mixing into liquid or creamed honey.
3. The food supplement containing cyanobacterium Spirulina platensis having at least 8.0% of the blue pigment phycocyanin on the dry matter basis, characteri zed in that cyanobacterium Spirulina platensis is in the form of air-dry biomass and additionally preserved with bee honey at the following ratio of ingredients (% by mass): 0.5 - 50 of air-dry cyanobacterium Spirulina platensis biomass, the rest is liquid or creamed bee honey.
4. The production method of the food supplement according to claim 3 characterized in that the cyanobacterium Spirulina platensis biomass is washed with drinking water, vacuumed to 70 - 75 % moisture, dried to an air-dry substance under 36-42 °C temperature; the air-dry cyanobacterium Spirulina platensis biomass is poured while mixing into liquid or creamed honey.
PCT/IB2010/053921 2009-09-02 2010-09-01 Algae spirulina platensis preservation by natural honey WO2011027301A1 (en)

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LT2009060A LT5657B (en) 2009-09-02 2009-09-02 Algae spirulina platensis preservation by natural honey

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US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
US8202425B2 (en) 2010-04-06 2012-06-19 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8211309B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of proteins by a two solvent method
US8211308B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of polar lipids by a two solvent method
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US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
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US8084038B2 (en) 2010-04-06 2011-12-27 Heliae Development, Llc Methods of and systems for isolating nutraceutical products from algae
US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
US8137555B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Methods of and systems for producing biofuels
US8137558B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Stepwise extraction of plant biomass for diesel blend stock production
US8137556B2 (en) 2010-04-06 2012-03-20 Heliae Development, Llc Methods of producing biofuels from an algal biomass
US8142659B2 (en) 2010-04-06 2012-03-27 Heliae Development, LLC. Extraction with fractionation of oil and proteinaceous material from oleaginous material
US8152870B2 (en) 2010-04-06 2012-04-10 Heliae Development, Llc Methods of and systems for producing biofuels
US8153137B2 (en) 2010-04-06 2012-04-10 Heliae Development, Llc Methods of and systems for isolating carotenoids and omega-3 rich oil products from algae
US8182556B2 (en) 2010-04-06 2012-05-22 Haliae Development, LLC Liquid fractionation method for producing biofuels
US8182689B2 (en) 2010-04-06 2012-05-22 Heliae Development, Llc Methods of and systems for dewatering algae and recycling water therefrom
US8187463B2 (en) 2010-04-06 2012-05-29 Heliae Development, Llc Methods for dewatering wet algal cell cultures
US8197691B2 (en) 2010-04-06 2012-06-12 Heliae Development, Llc Methods of selective removal of products from an algal biomass
US8202425B2 (en) 2010-04-06 2012-06-19 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8211309B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of proteins by a two solvent method
US8211308B2 (en) 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of polar lipids by a two solvent method
US8242296B2 (en) 2010-04-06 2012-08-14 Heliae Development, Llc Products from step-wise extraction of algal biomasses
US8273248B1 (en) 2010-04-06 2012-09-25 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8293108B1 (en) 2010-04-06 2012-10-23 Heliae Developmet, LLC Methods of and systems for producing diesel blend stocks
US8308950B2 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of dewatering algae for diesel blend stock production
US8308949B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of extracting neutral lipids and producing biofuels
US8308948B2 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Methods of selective extraction and fractionation of algal products
US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
US8313647B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Nondisruptive methods of extracting algal components for production of carotenoids, omega-3 fatty acids and biofuels
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
US8318019B2 (en) 2010-04-06 2012-11-27 Heliae Development, Llc Methods of dewatering algae for extraction of algal products
US8318018B2 (en) 2010-04-06 2012-11-27 Heliae Development, Llc Methods of extracting neutral lipids and recovering fuel esters
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