WO2009090991A1 - Centrifugal separator, and measuring-sample preparing method using the separator - Google Patents

Centrifugal separator, and measuring-sample preparing method using the separator Download PDF

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Publication number
WO2009090991A1
WO2009090991A1 PCT/JP2009/050452 JP2009050452W WO2009090991A1 WO 2009090991 A1 WO2009090991 A1 WO 2009090991A1 JP 2009050452 W JP2009050452 W JP 2009050452W WO 2009090991 A1 WO2009090991 A1 WO 2009090991A1
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tube
specimen
dilution
sample
centrifuge
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PCT/JP2009/050452
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French (fr)
Japanese (ja)
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Noriyuki Suzuki
Yoshunobu Hata
Shigeru Hiraoka
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Kyowa Medex Co., Ltd.
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Priority to JP2009550039A priority Critical patent/JP4944970B2/en
Publication of WO2009090991A1 publication Critical patent/WO2009090991A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components

Definitions

  • the present invention relates to a centrifuge, and more particularly to a completely new centrifuge capable of performing centrifuge and dilution in the same apparatus.
  • the present invention also relates to a method for preparing a measurement sample using the centrifugal separator of the present invention.
  • the present invention was devised in view of the current situation, and the object of the present invention is to collect a supernatant liquid in a settled state without fail even if the sample is rich in lipids and difficult to settle.
  • An object of the present invention is to provide a centrifugal separator that can also perform a conventional centrifugal separation process.
  • the present invention is also intended to provide a method for preparing a sedimented fraction and a supernatant, which are measurement samples, even for a specimen that is rich in lipids and difficult to sediment.
  • a centrifugal separator is provided with an even number of buckets that are arranged so as to face each other across a central axis of a rotor that rotates at a required speed, and to these buckets.
  • the stored centrifuge tube for holding the sample to be subjected to centrifugation and the dilution tube for holding the sample diluent, and the one bucket facing the above are equipped with a centrifuge tube and the other bucket is Attach a dilution tube, aspirate the sample dilution solution in the dilution tube, and add it to the sample in the centrifuge tube to dilute the sample at an arbitrary magnification with the sample dilution solution in the dilution tube for the purpose of the test. And a dispensing mechanism that can be used.
  • a centrifuge that can collect a supernatant liquid in a sedimented state even if it is a lipid-rich sample that is difficult to settle, and can also perform a conventional centrifugation process.
  • a method for preparing a sedimented fraction and a supernatant, which is a sample for measurement, is provided even for a specimen that contains a large amount of lipid and is difficult to settle.
  • Examples of the specimen to be subjected to centrifugation using the centrifugal separator of the present invention include whole blood and the like.
  • Whole blood may be treated with an anticoagulant such as heparin or EDTA.
  • Whole blood is separated into a fraction (e.g., a blood cell fraction) and a supernatant (e.g., serum or plasma fraction) precipitated by centrifugation using the centrifuge of the present invention.
  • the centrifuge of the present invention can be used independently as a centrifuge device by itself, but the centrifuge of the specimen and the separated sedimented fraction or supernatant are used as measurement samples in the sample. It can also be used as one component in an automatic analyzer that automatically measures the measurement components.
  • the centrifuge tube in the present invention is not particularly limited as long as it is provided in the centrifuge of the present invention, holds a specimen, and can withstand centrifugation.
  • the dilution tube in the present invention is not particularly limited as long as it is provided in the centrifuge of the present invention, holds the sample dilution liquid, and can withstand centrifugation.
  • the dispensing mechanism in the present invention has a function of sucking the sample diluent in the dilution tube and transferring it to the centrifuge tube.
  • the sample dilution liquid in the dilution tube aspirated by the dispensing mechanism is added to the sample in the centrifuge tube, and the sample in the centrifuge tube is diluted.
  • the specimen diluent in the present invention is held in a dilution tube and used to dilute the specimen.
  • the sample diluent include an aqueous medium such as a buffer solution.
  • the buffer include known buffers such as a Good buffer.
  • the method for preparing a measurement sample of the present invention is a method using the centrifugal separator of the present invention, and includes the following steps. [1] A step of attaching the centrifuge tube and the dilution tube to the bucket of the centrifuge so that the centrifuge tube and the dilution tube face each other; [2] A step of adding the specimen to the centrifuge tube; [3] A step of adding the sample diluent to the dilution tube; [4] A step of diluting the sample in the centrifuge tube by adding the sample diluent in the dilution tube to the centrifuge tube prepared in step [2] by a dispensing mechanism; [5] A step of centrifuging the diluted specimen in the centrifuge tube prepared in step [4]; [6] A step of removing the sedimented fraction or supernatant produced in the centrifuge tube in step [5].
  • the dilution of the sample with the sample diluent is controlled by a dilution control system, and it is possible to appropriately set the dilution factor according to the type of the sample.
  • the above steps [1] to [6] are controlled by a control system including a dilution control system, and the specimen is diluted, the diluted specimen is centrifuged, and the sedimented fraction or supernatant produced by centrifugation is removed. All are performed automatically, and an efficient measurement sample can be prepared.
  • the centrifugal separator of the present invention as one component in the automatic analyzer, not only efficient measurement sample preparation but also efficient measurement of the measurement components in the specimen becomes possible. .
  • a measurement component the component etc. which are measured with the conventional automatic analyzer are mentioned.
  • the centrifuge tube containing the sample diluted with the sample diluent and the dilution tube sucked the sample diluent are arranged in a state suitable for centrifugation.
  • the centrifugal separator of the present invention is one component in an automatic analyzer that automatically performs measurement sample preparation from a specimen and measurement component measurement using the prepared measurement sample. Can also be used.
  • the specimen can be retested. That is, when an abnormal measurement value is output in the first test and the abnormal measurement value is caused by turbidity of the specimen, the specimen with the abnormal measurement value is extracted by the control system, and the extracted specimen is A sample suitable for measurement is prepared by diluting with a specimen diluent, and the sample is supplied to a measurement system of an automatic analyzer for inspection.
  • the specimen extracted from the abnormal measurement value by the control system is diluted with the specimen diluent at the magnification calculated by the dilution control system, and the prepared diluted specimen is subjected to centrifugation. Also in this centrifugation, conditions such as the rotation time, the rotation speed, and the temperature are controlled by the control system.
  • FIG. 1 is a front view showing a schematic configuration of a centrifugal separator according to an example of an embodiment of the present invention
  • FIG. 2 is a plan view of the centrifugal separator
  • FIG. 3 is a side view of the centrifugal separator
  • FIG. 4 is a plan view showing the configuration of the rotor portion of the centrifugal separator.
  • 1 is a block
  • 2 is a rotor
  • 3 is a rotor shaft
  • 4 is a block cover
  • 5 is a motor bracket
  • 6 is a shutter guide block
  • 7 is a shutter
  • 8 is a cut plate
  • 9 is a sound insulation block
  • 10 is a rack
  • 11 is a bucket
  • 12 is a sensor bracket
  • 13 is a swing arm
  • 14 is an arm retainer
  • 15 is a rack guide shaft
  • 16 is a centrifuge tube insertion / removal port
  • Reference numeral 17 denotes a centrifuge tube
  • 18 denotes a dilution tube.
  • Reference numeral 20 is a DC motor
  • 21 is a timing pulley
  • 22 is a slit cam
  • 23 is a timing pulley
  • 24 is a spacer
  • 25 is a rotating shaft
  • 26 is a parallel pin
  • 27 is a stepping motor
  • Reference numeral 28 denotes a pinion
  • 29 denotes a bearing
  • 30 denotes a shim ring
  • 31 denotes a spring
  • 32 denotes an anti-vibration rubber
  • 33 denotes a bearing holder.
  • the centrifugal separator A is an even number (three pairs in this embodiment) of buckets that are arranged so as to face each other with the central axis O of the rotor 2 rotating at a required speed. 11 and a centrifuge tube 17 and a dilution tube 18 accommodated in these buckets 11.
  • One of the opposed buckets 11 is equipped with the centrifuge tube 17, and the other is a dilution tube. 18 is arranged, and the dilution tube 18 is used when dilution at an arbitrary magnification is performed corresponding to the inspection purpose.
  • Each bucket 11 is rotatably suspended by a rotating shaft 25 and is configured to hold the centrifuge tube 17 and the dilution tube 18 so as to be detachable.
  • the insertion / removal port 16 of the centrifuge tube 17 is controlled to be opened and closed by the shutter 7 and is opened when the centrifuge tube 17 is inserted / removed.
  • the insertion / removal port 16 is opened and diluted to a desired magnification.
  • the centrifuge tube 17 and the dilution tube 18 that have been subjected to the predetermined processing are picked up from the insertion / removal port 16 by a pickup arm or the like and discarded at a predetermined position.
  • the centrifugal separator In the centrifugal separator according to this embodiment, the greatest feature is that the centrifugal separation process can be performed, and the dilution process can also be performed at the same time. Since the supernatant can be collected, the analysis work that has been performed manually can be automatically performed. A dilution control system works in this dilution process, and the dilution rate can be set appropriately depending on the specimen.

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Abstract

Provided is a centrifugal separator, which can extract a supernatant liquid without fail in a sedimented state from a specimen, even if the specimen contains so much lipid that it is hard to sediment, and which can also perform the conventional centrifugal separation. The centrifugal separator is constituted to comprise even numbers of buckets arranged rotatably to confront each other across the center axis of a rotor rotating at a desired speed, and centrifugal pipes and diluting pipes housed in those buckets, for holding the specimens to be centrifugally separated and for holding a specimen diluting liquid. The centrifugal pipe is mounted in one of the confronting buckets, and the diluting pipe is mounted in the other confronting bucket. Further comprised is a dispensing mechanism for sucking an A-specimen diluting liquid in the diluting pipe and for adding the same to the specimen in the centrifugal pipe so that the specimen can be diluted at an arbitrary magnification with the specimen diluting liquid in the diluting pipe in accordance with an inspection purpose.

Description

遠心分離装置及び該装置を用いる測定用サンプルの調製方法Centrifugal separation device and method for preparing measurement sample using the same
　本発明は、遠心分離装置に係り、特に、遠心分離と希釈とを同一装置で行うことができる全く新規な遠心分離装置に関する。また、本発明は、本発明の遠心分離装置を用いる測定用サンプルの調製方法に関する。 The present invention relates to a centrifuge, and more particularly to a completely new centrifuge capable of performing centrifuge and dilution in the same apparatus. The present invention also relates to a method for preparing a measurement sample using the centrifugal separator of the present invention.
　周知のように、生化学的分析や免疫学的分析等の各種血液検査を行なう場合には、その前処理として血液の遠心分離作業が行なわれるが、従来のこの種の遠心分離器は、遠心分離血液が収容された遠心管を、回転バランスを考慮しながら人手を介して逐一遠心管ロータにセットし、この後スタートスイッチをオンして遠心管ロータを一定の速度で高速回転させ、所定時間経過後、オフスイッチをオン操作させることで上記遠心管ロータを停止させ、遠心管ロータが完全に停止したところで、人手によって遠心管ロータから遠心分離処理が終了した遠心管を取り外していた（特許文献１参照）。 As is well known, when various blood tests such as biochemical analysis and immunological analysis are performed, blood is centrifuged as a pretreatment. Set the centrifuge tube containing the separated blood to the centrifuge tube rotor one by one with manual rotation taking into account the rotation balance, and then turn on the start switch to rotate the centrifuge tube rotor at a constant speed for a predetermined time. After the elapse of time, the centrifuge tube rotor was stopped by turning on the off switch, and when the centrifuge tube rotor was completely stopped, the centrifuge tube that had been subjected to the centrifugal separation process was manually removed from the centrifuge tube rotor (Patent Literature). 1).
特開平０６－１８２２５７号公報Japanese Patent Laid-Open No. 06-182257
　しかしながら、検体の中には、脂質が多い検体もあり、このような検体の場合には遠心分離をしても沈降しにくいケースのものもあり、上澄み液を採取しにくいという問題を有していた。 However, some of the specimens are rich in lipids. In such specimens, there are cases in which sedimentation is difficult even after centrifugation, and it is difficult to collect the supernatant. It was.
　本発明は、かかる現状に鑑み創案されたものであって、その目的とするところは、脂質が多い沈降しにくい検体であっても、必ず沈降した状態で上澄み液を採取することができるとともに、従来の遠心分離処理も行うことができる遠心分離装置を提供しようとするものである。また、本発明は、脂質が多い沈降しにくい検体であっても、測定用サンプルである沈降した画分と上澄み液を調製する方法を提供しようとするものである。 The present invention was devised in view of the current situation, and the object of the present invention is to collect a supernatant liquid in a settled state without fail even if the sample is rich in lipids and difficult to settle. An object of the present invention is to provide a centrifugal separator that can also perform a conventional centrifugal separation process. The present invention is also intended to provide a method for preparing a sedimented fraction and a supernatant, which are measurement samples, even for a specimen that is rich in lipids and difficult to sediment.
　上記目的を達成するため、本発明にあっては、遠心分離装置を、所要速度で回転するロータの中心軸を挟んで対峙して回転自在に配置された偶数個のバケットと、これらのバケットに収納される、遠心分離に供される検体を保持するための遠心管及び検体希釈液を保持するための希釈管と、上記対峙する一方のバケットには遠心管を装着し、他方のバケットには希釈管を装着して、希釈管中の検体希釈液を吸引し、遠心管中の検体に添加することにより該希釈管中の検体希釈液で、検査目的に対応して検体を任意倍率で希釈することができる分注機構と、により構成したことを特徴とするものである。 In order to achieve the above object, according to the present invention, a centrifugal separator is provided with an even number of buckets that are arranged so as to face each other across a central axis of a rotor that rotates at a required speed, and to these buckets. The stored centrifuge tube for holding the sample to be subjected to centrifugation and the dilution tube for holding the sample diluent, and the one bucket facing the above are equipped with a centrifuge tube and the other bucket is Attach a dilution tube, aspirate the sample dilution solution in the dilution tube, and add it to the sample in the centrifuge tube to dilute the sample at an arbitrary magnification with the sample dilution solution in the dilution tube for the purpose of the test. And a dispensing mechanism that can be used.
　また、本発明にあっては、本発明の遠心分離装置を用いる測定用サンプルの調製方法であって、以下の工程を含むことを特徴とするものである。
［１］遠心管と希釈管とを、遠心管と希釈管とが対峙するように遠心分離装置のバケットに装着する工程；
［２］検体を遠心管に添加する工程；
［３］検体希釈液を希釈管に添加する工程；
［４］分注機構により、希釈管中の検体希釈液を、工程［２］で調製された遠心管に添加し、該遠心管中の検体を希釈する工程；
［５］工程［４］で調製した遠心管中の希釈された検体を遠心分離する工程；
［６］工程［５］により遠心管中に生成した沈降した画分又は上澄み液を取り出す工程。 Moreover, in this invention, it is the preparation method of the sample for a measurement using the centrifuge of this invention, Comprising: The following processes are included, It is characterized by the above-mentioned.
[1] A step of attaching the centrifuge tube and the dilution tube to the bucket of the centrifuge so that the centrifuge tube and the dilution tube face each other;
[2] A step of adding the specimen to the centrifuge tube;
[3] A step of adding the sample diluent to the dilution tube;
[4] A step of diluting the sample in the centrifuge tube by adding the sample diluent in the dilution tube to the centrifuge tube prepared in step [2] by a dispensing mechanism;
[5] A step of centrifuging the diluted specimen in the centrifuge tube prepared in step [4];
[6] A step of removing the sedimented fraction or supernatant produced in the centrifuge tube in step [5].
　脂質が多い沈降しにくい検体であっても、必ず沈降した状態で上澄み液を採取することができるとともに、従来の遠心分離処理も行うことができる遠心分離装置が提供される。また、脂質が多い沈降しにくい検体であっても、測定用サンプルである沈降した画分と上澄み液を調製する方法が提供される。 Provided is a centrifuge that can collect a supernatant liquid in a sedimented state even if it is a lipid-rich sample that is difficult to settle, and can also perform a conventional centrifugation process. In addition, a method for preparing a sedimented fraction and a supernatant, which is a sample for measurement, is provided even for a specimen that contains a large amount of lipid and is difficult to settle.
　本発明の遠心分離装置を用いる遠心分離に供される検体は、例えば全血等が挙げられる。全血はヘパリンやＥＤＴＡ等の抗凝固剤で処理されたものでもよい。全血は、本発明の遠心分離装置を用いる遠心分離により沈降した画分（例えば、血球画分）と上澄み液（例えば、血清又は血漿画分）とに分離される。 Examples of the specimen to be subjected to centrifugation using the centrifugal separator of the present invention include whole blood and the like. Whole blood may be treated with an anticoagulant such as heparin or EDTA. Whole blood is separated into a fraction (e.g., a blood cell fraction) and a supernatant (e.g., serum or plasma fraction) precipitated by centrifugation using the centrifuge of the present invention.
　本発明の遠心分離装置は、それ自体で独立に遠心分離用機器として使用することができるが、検体の遠心分離と、分離された沈降した画分又は上澄み液を測定用サンプルとして、当該サンプル中の測定成分の測定とを自動で行う自動分析装置の中の１つの構成要素として用いることもできる。
　本発明における遠心管は、本発明の遠心分離装置に具備され、検体を保持し、遠心分離に耐えられるものであれば特に制限はない。また、本発明における希釈管は、本発明の遠心分離装置に具備され、検体希釈液を保持し、遠心分離に耐えられるものであれば特に制限はない。 The centrifuge of the present invention can be used independently as a centrifuge device by itself, but the centrifuge of the specimen and the separated sedimented fraction or supernatant are used as measurement samples in the sample. It can also be used as one component in an automatic analyzer that automatically measures the measurement components.
The centrifuge tube in the present invention is not particularly limited as long as it is provided in the centrifuge of the present invention, holds a specimen, and can withstand centrifugation. In addition, the dilution tube in the present invention is not particularly limited as long as it is provided in the centrifuge of the present invention, holds the sample dilution liquid, and can withstand centrifugation.
　本発明における分注機構は、希釈管中の検体希釈液を吸引し、遠心管に移送する機能を有する。分注機構により吸引された希釈管中の検体希釈液は、遠心管中の検体に添加され、遠心管中の検体は希釈される。 The dispensing mechanism in the present invention has a function of sucking the sample diluent in the dilution tube and transferring it to the centrifuge tube. The sample dilution liquid in the dilution tube aspirated by the dispensing mechanism is added to the sample in the centrifuge tube, and the sample in the centrifuge tube is diluted.
　本発明における検体希釈液は、希釈管中に保持され、検体を希釈するために使用される。検体希釈液としては、例えば緩衝液等の水性媒体等が挙げられる。緩衝液としては、例えばグッド緩衝液等の公知の緩衝液等が挙げられる。 The specimen diluent in the present invention is held in a dilution tube and used to dilute the specimen. Examples of the sample diluent include an aqueous medium such as a buffer solution. Examples of the buffer include known buffers such as a Good buffer.
　本発明の測定用サンプルの調製方法は、本発明の遠心分離装置を用いる方法であり、以下の工程を含む。
［１］遠心管と希釈管とを、遠心管と希釈管とが対峙するように遠心分離装置のバケットに装着する工程；
［２］検体を遠心管に添加する工程；
［３］検体希釈液を希釈管に添加する工程；
［４］分注機構により、希釈管中の検体希釈液を、工程［２］で調製された遠心管に添加し、該遠心管中の検体を希釈する工程；
［５］工程［４］で調製した遠心管中の希釈された検体を遠心分離する工程；
［６］工程［５］により遠心管中に生成した沈降した画分又は上澄み液を取り出す工程。 The method for preparing a measurement sample of the present invention is a method using the centrifugal separator of the present invention, and includes the following steps.
[1] A step of attaching the centrifuge tube and the dilution tube to the bucket of the centrifuge so that the centrifuge tube and the dilution tube face each other;
[2] A step of adding the specimen to the centrifuge tube;
[3] A step of adding the sample diluent to the dilution tube;
[4] A step of diluting the sample in the centrifuge tube by adding the sample diluent in the dilution tube to the centrifuge tube prepared in step [2] by a dispensing mechanism;
[5] A step of centrifuging the diluted specimen in the centrifuge tube prepared in step [4];
[6] A step of removing the sedimented fraction or supernatant produced in the centrifuge tube in step [5].
　検体希釈液による検体の希釈は、希釈制御系により制御されており、検体の種類に合わせて適宜、希釈倍率を設定することが可能である。上記［１］～［６］の工程は、希釈制御系を含めた制御系で制御され、検体の希釈、希釈された検体の遠心分離、遠心分離により生成した沈降した画分又は上澄み液の取り出しは全て、自動で行われ、効率的な測定用サンプルの調製が可能となる。また、本発明の遠心分離装置を自動分析装置の中の１つの構成要素とすることにより、効率的な測定用サンプルの調製のみならず、検体中の測定成分の効率的な測定が可能となる。ここで、測定成分としては、従来の自動分析装置で測定されている成分等が挙げられる。 The dilution of the sample with the sample diluent is controlled by a dilution control system, and it is possible to appropriately set the dilution factor according to the type of the sample. The above steps [1] to [6] are controlled by a control system including a dilution control system, and the specimen is diluted, the diluted specimen is centrifuged, and the sedimented fraction or supernatant produced by centrifugation is removed. All are performed automatically, and an efficient measurement sample can be prepared. In addition, by using the centrifugal separator of the present invention as one component in the automatic analyzer, not only efficient measurement sample preparation but also efficient measurement of the measurement components in the specimen becomes possible. . Here, as a measurement component, the component etc. which are measured with the conventional automatic analyzer are mentioned.
　上記［４］工程の遠心分離において、検体希釈液で希釈された検体を含む遠心管と、検体希釈液を吸い取られた希釈管とは、遠心分離に適した状態に配置される。 In the centrifugation in the above [4] step, the centrifuge tube containing the sample diluted with the sample diluent and the dilution tube sucked the sample diluent are arranged in a state suitable for centrifugation.
　また、前述の通り、本発明の遠心分離装置は、検体から測定用サンプルの調製と、調製した測定用サンプルを用いた測定成分の測定とを自動で行う自動分析装置の中の１つの構成要素として用いることもできる。この様な自動分析装置を用いることにより、検体の再検査が可能となる。すなわち、１回目の検査で異常な測定値が出され、その異常な測定値が検体の濁りに起因する場合、異常な測定値が出された検体を制御系により抽出し、抽出された検体を検体希釈液で希釈して測定に適したサンプルを調製し、該サンプルは自動分析装置の測定系に供され、検査が行われる。ここで、異常な測定値から制御系により抽出された検体は、希釈制御系により算出された倍率で検体希釈液により希釈され、調製された希釈検体は遠心分離に供されるのである。この遠心分離においても、回転時間、回転速度、温度等の条件は制御系で制御される。 Further, as described above, the centrifugal separator of the present invention is one component in an automatic analyzer that automatically performs measurement sample preparation from a specimen and measurement component measurement using the prepared measurement sample. Can also be used. By using such an automatic analyzer, the specimen can be retested. That is, when an abnormal measurement value is output in the first test and the abnormal measurement value is caused by turbidity of the specimen, the specimen with the abnormal measurement value is extracted by the control system, and the extracted specimen is A sample suitable for measurement is prepared by diluting with a specimen diluent, and the sample is supplied to a measurement system of an automatic analyzer for inspection. Here, the specimen extracted from the abnormal measurement value by the control system is diluted with the specimen diluent at the magnification calculated by the dilution control system, and the prepared diluted specimen is subjected to centrifugation. Also in this centrifugation, conditions such as the rotation time, the rotation speed, and the temperature are controlled by the control system.
　以下、添付図面に示す一実施形態例に基づき、この発明を詳細に説明する。 Hereinafter, the present invention will be described in detail based on an embodiment shown in the accompanying drawings.
　図１は、この発明の一実施検体例に係る遠心分離装置の概略的な構成を示す正面図を、図２は同遠心分離装置の平面図を、図３は同遠心分離装置の側面図を、図４は同遠心分離装置のロータ部分の構成を示す平面図を夫々示している。 FIG. 1 is a front view showing a schematic configuration of a centrifugal separator according to an example of an embodiment of the present invention, FIG. 2 is a plan view of the centrifugal separator, and FIG. 3 is a side view of the centrifugal separator. FIG. 4 is a plan view showing the configuration of the rotor portion of the centrifugal separator.
　図中、符号１はブロックを、２はロータを、３はロータシャフトを、４はブロックカバーを、５はモータブラケットを、６はシャッターガイドブロックを、７はシャッターを、８はカット板を、９は遮音ブロックを、１０はラックを、１１はバケットを、１２はセンサーブラケットを、１３はスイングアームを、１４はアーム押さえを、１５はラックガイドシャフトを、１６は遠心管の挿脱口を、１７は遠心管を、１８は希釈管を夫々示している。 In the figure, 1 is a block, 2 is a rotor, 3 is a rotor shaft, 4 is a block cover, 5 is a motor bracket, 6 is a shutter guide block, 7 is a shutter, 8 is a cut plate, 9 is a sound insulation block, 10 is a rack, 11 is a bucket, 12 is a sensor bracket, 13 is a swing arm, 14 is an arm retainer, 15 is a rack guide shaft, 16 is a centrifuge tube insertion / removal port, Reference numeral 17 denotes a centrifuge tube, and 18 denotes a dilution tube.
　また、符号２０はＤＣモータを、２１はタイミングプーリーを、２２はスリットカムを、２３はタイミングプーリーを、２４はスペーサーを、２５は回転軸を、２６は平行ピンを、２７はステッピングモータを、２８はピニオンを、２９はベアリングを、３０はシムリングを、３１はスプリングを、３２は防振ゴムを、３３はベアリングホルダを夫々示している。 Reference numeral 20 is a DC motor, 21 is a timing pulley, 22 is a slit cam, 23 is a timing pulley, 24 is a spacer, 25 is a rotating shaft, 26 is a parallel pin, 27 is a stepping motor, Reference numeral 28 denotes a pinion, 29 denotes a bearing, 30 denotes a shim ring, 31 denotes a spring, 32 denotes an anti-vibration rubber, and 33 denotes a bearing holder.
　即ち、この実施形態例に係る遠心分離装置Ａは、所要速度で回転するロータ２の中心軸Ｏを挟んで対峙して回転自在に配置された偶数個（本実施形態例では３対）のバケット１１と、これらのバケット１１に収納される遠心管１７及び希釈管１８と、を有して構成され、対峙するバケット１１の一方には上記遠心管１７が装着され、また、他方には希釈管１８を配置し、該希釈管１８は、検査目的に対応して任意倍率の希釈を行う場合に使用される。 In other words, the centrifugal separator A according to this embodiment is an even number (three pairs in this embodiment) of buckets that are arranged so as to face each other with the central axis O of the rotor 2 rotating at a required speed. 11 and a centrifuge tube 17 and a dilution tube 18 accommodated in these buckets 11. One of the opposed buckets 11 is equipped with the centrifuge tube 17, and the other is a dilution tube. 18 is arranged, and the dilution tube 18 is used when dilution at an arbitrary magnification is performed corresponding to the inspection purpose.
　各バケット１１は、回転軸２５に回動自在に吊持され、遠心管１７及び希釈管１８を挿脱自在に保持するように構成されている。 Each bucket 11 is rotatably suspended by a rotating shaft 25 and is configured to hold the centrifuge tube 17 and the dilution tube 18 so as to be detachable.
　遠心管１７の挿脱口１６は、シャッター７によって開閉制御され、遠心管１７の挿脱時には開くと共に、該位置に希釈管１８が到達した場合にも開いて任倍率への希釈処理が行われる。勿論、所定の処理が終了した遠心管１７・希釈管１８は、該挿脱口１６からピックアップアームなどでピックアップされ所定の位置に廃棄される。 The insertion / removal port 16 of the centrifuge tube 17 is controlled to be opened and closed by the shutter 7 and is opened when the centrifuge tube 17 is inserted / removed. When the dilution tube 18 reaches the position, the insertion / removal port 16 is opened and diluted to a desired magnification. Of course, the centrifuge tube 17 and the dilution tube 18 that have been subjected to the predetermined processing are picked up from the insertion / removal port 16 by a pickup arm or the like and discarded at a predetermined position.
　この実施形態例に係る遠心分離装置において、最大の特徴は、遠心分離処理ができることは勿論、希釈処理も同時に行うことができ、特に、沈降しにくい血液試料であっても、遠心処理によって必ず沈降してその上澄み液を採取できるので、従来手作業で行っていた分析作業を、自動的に行うことが可能となる点にある。そして、この希釈処理には希釈制御系が働き、検体により希釈率を適宜設定することが可能となる。 In the centrifugal separator according to this embodiment, the greatest feature is that the centrifugal separation process can be performed, and the dilution process can also be performed at the same time. Since the supernatant can be collected, the analysis work that has been performed manually can be automatically performed. A dilution control system works in this dilution process, and the dilution rate can be set appropriately depending on the specimen.
　尚、本実施形態例における遠心分離装置の基本的構成及び作動制御は従来の遠心分離装置と同様であるので、その詳細な説明をここでは省略する。また、分注機構についても基本的構成及び作動制御は従来の分注装置と同様であるので、図面上の図示及び詳細な説明を省略する。 Note that the basic configuration and operation control of the centrifugal separator in this embodiment are the same as those of the conventional centrifugal separator, and thus detailed description thereof is omitted here. Further, the basic structure and operation control of the dispensing mechanism are the same as those of the conventional dispensing device, and therefore illustrations and detailed descriptions on the drawings are omitted.
この発明の一実施検体例に係る遠心分離装置の概略的な構成を示す正面図である。It is a front view which shows schematic structure of the centrifuge which concerns on the example of 1 execution sample of this invention. 同遠心分離装置の平面図である。It is a top view of the centrifuge. 同遠心分離装置の側面図である。It is a side view of the centrifuge. 同遠心分離装置のロータ部分の構成を示す平面図である。It is a top view which shows the structure of the rotor part of the centrifuge.
符号の説明Explanation of symbols
　Ａ　遠心分離装置
　Ｏ　ロータの中心軸
　２　ロータ
　７　シャッター
　１１　バケット
　１６　挿脱口
　１７　遠心管
　１８　希釈管 A Centrifugal Separator O Central axis of rotor 2 Rotor 7 Shutter 11 Bucket 16 Insertion / removal port 17 Centrifugal tube 18 Dilution tube

Claims (2)

  1. 　所要速度で回転するロータの中心軸を挟んで対峙して回転自在に配置された偶数個のバケットと、これらのバケットに収納される、遠心分離に供される検体を保持するための遠心管及び検体希釈液を保持するための希釈管と、上記対峙する一方のバケットには遠心管を装着し、他方のバケットには希釈管を装着して、希釈管中の検体希釈液を吸引し、遠心管中の検体に添加することにより該希釈管中の検体希釈液で、検査目的に対応して検体を任意倍率で希釈することができる分注機構とを有する遠心分離装置。 An even number of buckets that are rotatably arranged across the central axis of a rotor that rotates at a required speed, and a centrifuge tube that is housed in these buckets and holds a specimen to be centrifuged A centrifuge tube is attached to the dilution tube for holding the sample dilution solution and one of the above buckets, and a dilution tube is attached to the other bucket, and the sample dilution solution in the dilution tube is aspirated and centrifuged. A centrifuge having a dispensing mechanism capable of diluting a sample at an arbitrary magnification in accordance with a test purpose with a sample dilution liquid in the dilution tube by adding to the sample in the tube.
  2. 　以下の工程を含むことを特徴とする、請求項１記載の遠心分離装置を用いる測定用サンプルの調製方法。
    ［１］遠心管と希釈管とを、遠心管と希釈管とが対峙するように遠心分離装置のバケットに装着する工程；
    ［２］検体を遠心管に添加する工程；
    ［３］検体希釈液を希釈管に添加する工程；
    ［４］分注機構により、希釈管中の検体希釈液を、工程［２］で調製された遠心管に添加し、該遠心管中の検体を希釈する工程；
    ［５］工程［４］で調製した遠心管中の希釈された検体を遠心分離する工程；
    ［６］工程［５］により遠心管中に生成した沈降した画分又は上澄み液を取り出す工程。     The method for preparing a measurement sample using the centrifugal separator according to claim 1, comprising the following steps.
    [1] A step of attaching the centrifuge tube and the dilution tube to the bucket of the centrifuge so that the centrifuge tube and the dilution tube face each other;
    [2] A step of adding the specimen to the centrifuge tube;
    [3] A step of adding the sample diluent to the dilution tube;
    [4] A step of diluting the sample in the centrifuge tube by adding the sample diluent in the dilution tube to the centrifuge tube prepared in step [2] by a dispensing mechanism;
    [5] A step of centrifuging the diluted specimen in the centrifuge tube prepared in step [4];
    [6] A step of removing the sedimented fraction or supernatant produced in the centrifuge tube in step [5].
PCT/JP2009/050452 2008-01-15 2009-01-15 Centrifugal separator, and measuring-sample preparing method using the separator WO2009090991A1 (en)

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CN108187922B (en) * 2017-12-25 2020-11-27 泗县智来机械科技有限公司 Test tube centrifugal mechanism convenient to place

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