WO2003072818A3 - Method for sorting single-stranded nucleic acids - Google Patents

Method for sorting single-stranded nucleic acids Download PDF

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Publication number
WO2003072818A3
WO2003072818A3 PCT/EP2003/002025 EP0302025W WO03072818A3 WO 2003072818 A3 WO2003072818 A3 WO 2003072818A3 EP 0302025 W EP0302025 W EP 0302025W WO 03072818 A3 WO03072818 A3 WO 03072818A3
Authority
WO
WIPO (PCT)
Prior art keywords
stranded nucleic
nucleic acid
nucleic acids
cells
invariable
Prior art date
Application number
PCT/EP2003/002025
Other languages
German (de)
French (fr)
Other versions
WO2003072818A2 (en
Inventor
Dirk Boekenkamp
Tom Heike Dieck
Hans-Ulrich Hoppe
Original Assignee
Degussa Bioactives Gmbh
Dirk Boekenkamp
Tom Heike Dieck
Hans-Ulrich Hoppe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa Bioactives Gmbh, Dirk Boekenkamp, Tom Heike Dieck, Hans-Ulrich Hoppe filed Critical Degussa Bioactives Gmbh
Priority to AU2003214078A priority Critical patent/AU2003214078A1/en
Publication of WO2003072818A2 publication Critical patent/WO2003072818A2/en
Publication of WO2003072818A3 publication Critical patent/WO2003072818A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for sorting single-stranded nucleic acids by isolating and hybridizing suitable nucleic acid pools followed by a selection. According to the invention, the single-stranded nucleic acids are selectively bound with the aid of capturing substances (capture), which are immobilized on a solid matrix surface and which have variable and invariable areas. This method is characterized in that the capturing substances are oligonucleotides having 5'-terminal invariable anchor sequences that, in complementary form, occur at least once in each single-stranded nucleic acid, and having 3'-terminal variable discriminating sequences consisting of every possible sequence of ≤ 10 nucleotides, which enable the formation of certain types of single-stranded nucleic acids. The invention also relates to the use of this method for analyzing single-stranded nucleic acid patterns, particularly in mucous membrane, hair root, blood, nerve or reproduction cells, and to the use for detecting the action of pharmaceutically and/or nutritional-physiologically active compounds. This novel method makes it possible, in a quick and economical manner, to depict reproducible differences in the single-stranded nucleic acid pool of cells.
PCT/EP2003/002025 2002-02-28 2003-02-27 Method for sorting single-stranded nucleic acids WO2003072818A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003214078A AU2003214078A1 (en) 2002-02-28 2003-02-27 Method for sorting single-stranded nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002108794 DE10208794A1 (en) 2002-02-28 2002-02-28 Methods for sorting single-stranded nucleic acids
DE10208794.6 2002-02-28

Publications (2)

Publication Number Publication Date
WO2003072818A2 WO2003072818A2 (en) 2003-09-04
WO2003072818A3 true WO2003072818A3 (en) 2004-03-11

Family

ID=27675126

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002025 WO2003072818A2 (en) 2002-02-28 2003-02-27 Method for sorting single-stranded nucleic acids

Country Status (3)

Country Link
AU (1) AU2003214078A1 (en)
DE (1) DE10208794A1 (en)
WO (1) WO2003072818A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
WO2000026412A1 (en) * 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2000056920A1 (en) * 1999-03-18 2000-09-28 Exiqon A/S One step sample preparation and detection of nucleic acids in complex biological samples
EP1146331A1 (en) * 1999-10-20 2001-10-17 Shigeori Takenaka Gene detecting chip, detector, and detecting method
EP1180548A2 (en) * 2000-07-31 2002-02-20 Agilent Technologies, Inc. Array based methods for synthesizing nucleic acid mixtures
EP1186673A2 (en) * 2000-09-11 2002-03-13 Agilent Technologies Inc Calibration of molecular array data

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
WO2000026412A1 (en) * 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2000056920A1 (en) * 1999-03-18 2000-09-28 Exiqon A/S One step sample preparation and detection of nucleic acids in complex biological samples
EP1146331A1 (en) * 1999-10-20 2001-10-17 Shigeori Takenaka Gene detecting chip, detector, and detecting method
EP1180548A2 (en) * 2000-07-31 2002-02-20 Agilent Technologies, Inc. Array based methods for synthesizing nucleic acid mixtures
EP1186673A2 (en) * 2000-09-11 2002-03-13 Agilent Technologies Inc Calibration of molecular array data

Also Published As

Publication number Publication date
AU2003214078A1 (en) 2003-09-09
WO2003072818A2 (en) 2003-09-04
DE10208794A1 (en) 2003-09-04

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