WO1998019689A9 - Novel coding sequences - Google Patents

Abstract

This invention relates to newly identified Streptococcal polynucleotides, polypeptides encoded by such polynucleotides, the uses of such polynucleotides and polypeptides, as well as the production of such polynucleotides and polypeptides and recombinant host cells transformed with the polynucleotides. This invention also relates to inhibiting the biosynthesis or action of such polynucleotides or polypeptides and to the use of such inhibitors in therapy.

Description

NOVEL CODING SEQUENCES FIELD OF THE INVENTION

This invention relates to newly identified polynucleotides and polypeptides, and their production and uses, as well as their variants, agonists and antagonists, and their uses. In particular, in these and in other regards, the invention relates to novel polynucleotides and polypeptides set forth in Table 1. BACKGROUND OF THE INVENTION

The Streptococci make up a medically important genera of microbes known to cause several types of disease in humans, including otitis media, pneumonia and meningitis. Since its isolation more than 100 years ago, Streptococcus pneumoniae (herein S. pneumoniae) has been one of the more intensively studied microbes. For example, much of our early understanding that DNA is, in fact, the genetic material was predicated on the work of Griffith and of Avery, Macleod and McCarty using this microbe. Despite the vast amount of research with S. pneumoniae, many questions concerning the virulence of this microbe remain.

While certain Streptococcal factors associated with pathogenicity have been identified, e.g., capsule polysaccharides, peptidoglycans, pneumolysins, PspA Complement factor H binding component, autolysin, neuraminidase, peptide permeases, hydrogen peroxide, IgAl protease, the list is certainly not complete. Further very little is known concerning the temporal expression of such genes during infection and disease progression in a mammalian host. Discovering the sets of genes the bacterium is likely to be expressing at the different stages of infection, particularly when an infection is established, provides critical information for the screening and characterization of novel antibacterials which can interrupt pathogenesis. In addition to providing a fuller understanding of known proteins, such an approach will identify previously unrecognised targets.

GUG is used as an initating nucleotide, rather than ATG, for a significant number of mRNA's in both Gram positive and Gram negative bacteria. Statistics on the frequency of NTG codons in the start codon for several bacterial species are available on line via computer at http://biochem.otago.ac.nz:800/Transterm/home_page.html).

A discussion of initiation codons in B. subtilis is set forth in Vellanoweth, RL.1993 in Bacillus subtilis and other Gram Positive Bacteria. Biochemistry. Physiology and Molecular Genetics. Sonenshein, Hoch, Losick Eds. Amer. Soc. Microbiol, Washington DC. p. 699-711. Vellenworth indicates a major difference between B. subtilis and the

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SUBSTmπΕ SHEET (RULE 26) gram-negative organisms is in the choice of initiation codon. 91% of the sequenced E. coli genes start with AUG. By contrast, about 30% of B. subtilis and other clostridial branch gened start with UUG or GUG. Moreover, CUG functions as a start codon in B. subtilis. Mutations of an AUG initiation codon to GUG or UUG often cause decreased expression in B. subtilis and E. coli. Generally, translation efficiency is higher with AUG initiation codons. A strong Shine-Delgarno ribosome binding site, however, can compensate almost fully for a weak initiation codon. It has been reported that genes with a range of expression levels have initiation codons other than ATG in gram positives (Vellanoweth, RL.1993 in Bacillus subtilis and other Gram Positive Bacteria. Biochemistry. Physiology and Molecular Genetics. Sonenshein, Hoch, Losick Eds. Amer. Soc. Microbiol, Washington DC. p. 699-711).

Provided herein are ORF sequences from genes possessing GUG initiation codons and proteins expressed therefrom and homologues thereto to be used for screening for antimicrobial compounds. Clearly, there is a need for polypeptide and polynucleotide sequences that may be used to screen for antimicrobial compound and which may also be used to determine the roles of such sequences in pathogenesis of infection, dysfunction and disease. There is also need, therefore, for identification and characterization of such sequences which may play a role in preventing, ameliorating or correcting infections, dysfunctions or diseases.

The polypeptides of the invention have amino acid sequence homology to a known protein(s) as set forth in Table 1. SUMMARY OF THE INVENTION

It is an object of the invention to provide polypeptides that have been identified as novel polypeptides by homology between an amino acid sequence selected from the group consisting of the sequences set out in Table 1 and a known amino acid sequence or sequences of other proteins such as the protein identities listed in Table 1.

It is a further object of the invention to provide polynucleotides that encode novel polypeptides, particularly polynucleotides that encode polypeptides of Streptococcus pneumoniae.

In a particularly preferred embodiment of the invention the polynucleotide comprises a region encoding a polypeptide comprising a sequence sequence selected from the group consisting of the sequences set out in Table 1, or a variant of any of these sequences.

SUBSπniTΕ SHEET (RULE 26) In another particularly preferred embodiment of the invention there is a novel protein from Streptococcus pneumoniae comprising an amino acid sequence selected from the group consisting of the sequences set out in Table 1, or a variant of any of these sequences.

In accordance with another aspect of the invention there is provided an isolated nucleic acid molecule encoding a mature polypeptide expressible by the Streptococcus pneumoniae 0100993 strain contained in the deposited strain.

A further aspect of the invention there are provided isolated nucleic acid molecules encoding a polypeptide of the invention, particularly Streptococcus pneumoniae polypeptide, and including mRNAs, cDNAs, genomic DNAs. Further embodiments of the invention include biologically, diagnostically, prophylactically, clinically or therapeutically useful variants thereof, and compositions comprising the same.

In accordance with another aspect of the invention, there is provided the use of a polynucleotide of the invention for therapeutic or prophylactic purposes, in particular genetic immunization. Among the particularly preferred embodiments of the invention are naturally occurring allelic variants of a polypeptide of the invention and polypeptides encoded thereby.

Another aspect of the invention there are provided novel polypeptides of Streptococcus pneumoniae as well as biologically, diagnostically, prophylactically, clinically or therapeutically useful variants thereof, and compositions comprising the same.

Among the particularly preferred embodiments of the invention are variants of the polypeptides of the invention encoded by naturally occurring alleles of their genes.

In a preferred embodiment of the invention there are provided methods for producing the aforementioned polypeptides.

In accordance with yet another aspect of the invention, there are provided inhibitors to such polypeptides, useful as antibacterial agents, including, for example, antibodies.

In accordance with certain preferred embodiments of the invention, there are provided products, compositions and methods for assessing expression of the polypeptides and polynucleotides of the invention, treating disease, for example, including, for example, otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid, assaying genetic variation, and administering a polypeptide or polynucleotide of the

SUBSTTTiπΕ SHEET (RULE 26) invention to an organism to raise an immunological response against a bacteria, especially a Streptococcus pneumoniae bacteria.

In accordance with certain preferred embodiments of this and other aspects of the invention there are provided polynucleotides that hybridize to a polynucleotide sequence of the invention, particularly under stringent conditions.

In certain preferred embodiments of the invention there are provided antibodies against polypeptides of the invention.

In other embodiments of the invention there are provided methods for identifying compounds which bind to or otherwise interact with and inhibit or activate an activity of a polypeptide or polynucleotide of the invention comprising: contacting a polypeptide or polynucleotide of the invention with a compound to be screened under conditions to permit binding to or other interaction between the compound and the polypeptide or polynucleotide to assess the binding to or other interaction with the compound, such binding or interaction being associated with a second component capable of providing a detectable signal in response to the binding or interaction of the polypeptide or polynucleotide with the compound; and determining whether the compound binds to or otherwise interacts with and activates or inhibits an activity of the polypeptide or polynucleotide by detecting the presence or absence of a signal generated from the binding or interaction of the compound with the polypeptide or polynucleotide.

In accordance with yet another aspect of the invention, there are provided agonists and antagonists of the polypeptides and polynucleotides of the invention, preferably bacteriostatic or bacteriocidal agonists and antagonists.

In a further aspect of the invention there are provided compositions comprising a polynucleotide or a polypeptide of the invention for administration to a cell or to a multicellular organism.

Various changes and modifications within the spirit and scope of the disclosed invention will become readily apparent to those skilled in the art from reading the following descriptions and from reading the other parts of the present disclosure. GLOSSARY

The following definitions are provided to facilitate understanding of certain terms used frequently herein.

SϋBSπ JTE SHEET (RULE 26) "Disease(s) means any bacterial infection, but preferably a streptococcal infection, such as, otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema. endocarditis, meningitis, and infection of cerebrospinal fluid.

"Host cell" is a cell which has been transformed or transfected, or is capable of transformation or transfection by an exogenous polynucleotide sequence.

"Identity," as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, "identity" also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. "Identity" and "similarity" can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A.M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D.W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A.M., and Griffin, H.G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991 ; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988). Preferred methods to determine identity are designed to give the largest match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Preferred computer program methods to determine identity and similarity between two sequences include, but are not limited to, the GCG program package (Devereux, J., et al., Nucleic Acids Research 12(1): 387 (1984)), BLASTP, BLASTN, and FASTA (Atschul, S.F. et al., J. Molec. Biol 215: 403-410 (1990). The BLAST X program is publicly available from NCBI and other sources (BLAST Manual, Altschul, S., et al, NCBI NLM NIH Bethesda, MD 20894; Altschul, S., et al, J. Mol Biol 215: 403-410 (1990). As an illustration, by a polynucleotide having a nucleotide sequence having at least, for example, 95% "identity" to a reference nucleotide sequence it is intended that the nucleotide sequence of the tested polynucleotide is identical to the reference sequence except that the polynucleotide sequence may include up to five point mutations per each 100 nucleotides of the reference nucleotide sequence. In other words, to obtain a polynucleotide having a nucleotide sequence at least 95% identical to a reference nucleotide sequence, up to 5% of the nucleotides in the reference sequence may be deleted or substituted with another

suBSimπ* SHEET (RULE 26) nucleotide, or a number of nucleotides up to 5% of the total nucleotides in the reference sequence may be inserted into the reference sequence. These mutations of the reference sequence may occur at the 5' or 3' terminal positions of the reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence. Analogously , by a polypeptide having an amino acid sequence having at least, for example, 95% identity to a reference amino acid sequence is intended that the test amino acid sequence of the polypeptide is identical to the reference sequence except that the polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the reference amino acid. In other words, to obtain a polypeptide having an amino acid sequence at least 95% identical to a reference amino acid sequence, up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or a number of amino acids up to 5% of the total amino acid residues in the reference sequence may be inserted into the reference sequence. These alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.

"Isolated" means altered "by the hand of man" from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or a polypeptide naturally present in a living organism is not "isolated," but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is "isolated", as the term is employed herein.

"Polynucleotide(s)" generally refers to any polyribonucleotide or polydeoxribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA. "Polynucleotide(s)" include, without limitation, single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions or single-, double- and triple-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double- stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded, or triple-stranded regions, or a mixture of single- and double- stranded regions. In addition, "polynucleotide" as used herein refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The strands in such regions may be from the same molecule or from different molecules. The regions may include all of one or more

SUBS TTUTΕ SHEET (RULE 26) of the molecules, but more typically involve only a region of some of the molecules. One of the molecules of a triple-helical region often is an oligonucleotide. As used herein, the term "polynucleotide(s)" also includes DNAs or RNAs as described above that contain one or more modified bases. Thus, DNAs or RNAs with backbones modified for stability or for other reasons are "polynucleotide(s)" as that term is intended herein. Moreover, DNAs or RNAs comprising unusual bases, such as inosine, or modified bases, such as tritylated bases, to name just two examples, are polynucleotides as the term is used herein. It will be appreciated that a great variety of modifications have been made to DNA and RNA that serve many useful purposes known to those of skill in the art. The term "polynucleotide(s)" as it is employed herein embraces such chemically, enzymatically or metabolically modified forms of polynucleotides, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including, for example, simple and complex cells. "Polynucleotide(s)" also embraces short polynucleotides often referred to as oligonucleotide(s).

"Polypeptide(s)" refers to any peptide or protein comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds. "Polypeptide(s)" refers to both short chains, commonly referred to as peptides, oligopeptides and oligomers and to longer chains generally referred to as proteins. Polypeptides may contain amino acids other than the 20 gene encoded amino acids. "Polypeptide(s)" include those modified either by natural processes, such as processing and other post-translational modifications, but also by chemical modification techniques. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature, and they are well known to those of skill in the art. It will be appreciated that the same type of modification may be present in the same or varying degree at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Modifications can occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains, and the amino or carboxyl termini. Modifications include, for example, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cysteine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, glycosylation,

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SUBST TUTE SHEET (RULE 26) lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins, such as arginylation, and ubiquitination. See, for instance, PROTEINS - STRUCTURE AND MOLECULAR PROPERTIES, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York (1993) and Wold, F., Posttranslational Protein Modifications: Perspectives and Prospects, pgs. 1-12 in POSTTRANSLATIONAL COVALENT MODIFICATION OF PROTEINS, B. C. Johnson, Ed., Academic Press, New York (1983); Seifter et al., Meth. Enzymol 182:626-646 (1990) and Rattan et al., Protein Synthesis: Posttranslational Modifications and Aging, Ann. N.Y. Acad. Sci. 663: 48-62 (1992). Polypeptides may be branched or cyclic, with or without branching. Cyclic, branched and branched circular polypeptides may result from post-translational natural processes and may be made by entirely synthetic methods, as well.

"Variant(s)" as the term is used herein, is a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide respectively, but retains essential properties. A typical variant of a polynucleotide differs in nucleotide sequence from another, reference polynucleotide. Changes in the nucleotide sequence of the variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from another, reference polypeptide. Generally, differences are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, additions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. A variant of a polynucleotide or polypeptide may be a naturally occurring such as an allelic variant, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques, by direct synthesis, and by other recombinant methods known to skilled artisans. DESCRIPTION OF THE INVENTION

Each of polynucleotide and polypeptide sequences provided herein may be used in the discovery and development of antibacterial compounds. Upon expression of the sequences with the appropriate initiation and termination codons the encoded polypeptide can be used as a target for the screening of antimicrobial drugs. Additionally, the DNA sequences encoding preferably the amino terminal regions of the encoded protein or the Shine-Delgarno region can be used to construct antisense sequences to control the expression of the coding sequence of interest. Furthermore, many of the sequences disclosed herein also provide regions upstream and downstream from the encoding sequence. These sequences are useful as a source of regulatory elements for the control of bacterial gene expression. Such sequences are conveniently isolated by restriction enzyme action or synthesized chemically and introduced, for example, into promoter identification strains. These strains contain a reporter structural gene sequence located downstream from a restriction site such that if an active promoter is inserted, the reporter gene will be expressed.

Although each of the sequences may be employed as described above, this invention also provides several means for identifying particularly useful target genes. The first of these approaches entails searching appropriate databases for sequence matches in related organisms. Thus, if a homologue exists, the Streptococcal-like form of this gene would likely play an analogous role. For example, a Streptococcal protein identified as homologous to a cell surface protein in another organism would be useful as a vaccine candidate. To the extent such homologies have been identified for the sequences disclosed herein they are reported along with the encoding sequence.

Each of the DNA sequences provided herein may be used in the discovery and development of antibacterial compounds. Because each of the sequences contains an open reading frame (ORF) with an appropriate initiation and termination codons, the encoded protein upon expression can be used as a target for the screening of antimicrobial drugs. Additionally, the DNA sequences encoding the amino terminal regions of the encoded protein can be used to construct antisense sequences to control the expression of the coding sequence of interest. Furthermore, many of the sequences disclosed herein also provide regions upstream and downstream from the encoding sequence. These sequences are useful as a source of regulatory elements for the control of bacterial gene expression. Such sequences are conveniently isolated by restriction enzyme action or synthesized chemically and introduced, for example, into promoter identification strains. These strains contain a reporter structural gene sequence located downstream from a restriction site such that if an active promoter is inserted, the reporter gene will be expressed.

SUBSTTTUTΈ SHEET (RULE 26) It is believed that bacteria possess a number of ways of regulating gene expression levels, especially in subtle degrees, and the interplay between ribosome binding site and inititation codon is utilized for this purpose for these genes. It is also believed that such genes will be important targets for antimicrobial drug discovery, particularly since pathogenesis genes are believed undergo gene expression regulation during in the pathogenesis process. Therefore, the invention provides ORF sequences possessing a GTG (GUG ) initiation codon and protein targets expressed thereform.

Although each of the sequences may be employed as described above, this invention also provides several means for identifying particularly useful target genes. The first of these approaches entails searching appropriate databases for sequence matches in related organisms. Thus, if a homologue exists, the Streptococcal-like form of this gene would likely play an analogous role. For example, a Streptococcal protein identified as homologous to a cell surface protein in another organism would be useful as a vaccine candidate. To the extent such homologies have been identified for the sequences disclosed herein they are reported along with the encoding sequence.

ORF Gene Expression

Recently techniques have become available to evaluate temporal gene expression in bacteria, particularly as it applies to viability under laboratory and infection conditions. A number of methods can be used to identify genes which are essential to survival per se, or essential to the establishment/maintenance of an infection. Identification of an ORF unknown by one of these methods yields additional information about its function and permits the selection of such an ORF for further development as a screening target. Briefly, these approaches include:

1) Signature Tagged Mutagenesis (STM): This technique is described by Hensel et al., Science 269: 400-403(1995), the contents of which is incorporated by reference for background purposes. Signature tagged mutagenesis identifies genes necessary for the establishment/maintenance of infection in a given infection model.

The basis of the technique is the random mutagenesis of target organism by various means (e.g., transposons) such that unique DNA sequence tags are inserted in close proximity to the site of mutation. The tags from a mixed population of bacterial mutants and bacteria recovered from an infected hosts are detected by amplification, radiolabeling and hybridisation analysis. Mutants attenuated in virulence are revealed by absence of the tag from the pool of bacteria recovered from infected hosts. In Streptococcus pneumoniae, because the transposon system is less well developed, a more efficient way of creating the tagged mutants is to use the insertion- duplication mutagenesis technique as described by Morrison et al., Bacteriol. 159:870 (1984) the contents of which is incorporated by reference for background purposes.

2) In Vivo Expression Technology (IVET): This technique is described by Camilli et aL, Proc. Nat'l. Acad. Sci. USA. 91:2634-2638 (1994), the contents of which is incorporated by reference for background purposes. IVET identifies genes up-regulated during infection when compared to laboratory cultivation, implying an important role in infection. ORF identified by this technique are implied to have a significant role in infection establishment/maintenance.

In this technique random chromosomal fragments of target organism are cloned upstream of a promoter-less recombinase gene in a plasmid vector. This construct is introduced into the target organism which carries an antibiotic resistance gene flanked by resolvase sites. Growth in the presence of the antibiotic removes from the population those fragments cloned into the plasmid vector capable of supporting transcription of the recombinase gene and therefore have caused loss of antibiotic resistance. The resistant pool is introduced into a host and at various times after infection bacteria may be recovered and assessed for the presence of antibiotic resistance. The chromosomal fragment carried by each antibiotic sensitive bacterium should carry a promoter or portion of a gene normally upregulated during infection. Sequencing upstream of the recombinase gene allows identification of the up regulated gene.

3) Differential display: This technique is described by Chuang et al., _ Bacteriol. 175:2026-2036 (1993), the contents of which is incorporated by reference for background purposes. This method identifies those genes which are expressed in an organism by identifying mRNA present using randomly-primed RT-PCR. By comparing pre-infection and post infection profiles, genes up and down regulated during infection can be identified and the RT-PCR product sequenced and matched to ORF 'unknowns'.

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SUBSTTTUTE SHEET (RULE 26) 4) Generation of conditional lethal mutants by transposon mutagenesis:

This technique, described by de Lorenzo, V. et al., Gene 123:17-24 (1993); Neuwald, A. F. et al., Gene 125: 69-73(1993); and Takiff, H. E. et ah, J. Bacteriol. 174:1544- 1553(1992), the contents of which is incorporated by reference for background purposes, identifies genes whose expression are essential for cell viability.

In this technique transposons carrying controllable promoters, which provide transcription outward from the transposon in one or both directions, are generated. Random insertion of these transposons into target organisms and subsequent isolation of insertion mutants in the presence of inducer of promoter activity ensures that insertions which separate promoter from coding region of a gene whose expression is essential for cell viability will be recovered. Subsequent replica plating in the absence of inducer identifies such insertions, since they fail to survive. Sequencing of the flanking regions of the transposon allows identification of site of insertion and identification of the gene disrupted. Close monitoring of the changes in cellular processes/morphology during growth in the absence of inducer yields information on likely function of the gene. Such monitoring could include flow cytometry (cell division, lysis, redox potential, DNA replication), incorporation of radiochemically labeled precursors into DNA, RNA, protein, lipid, peptidoglycan, monitoring reporter enzyme gene fusions which respond to known cellular stresses.

5) Generation of conditional lethal mutants by chemical mutagenesis: This technique is described by Beckwith, J.. Methods in Enzvmology 204:

3-18(1991), the contents of which are incorporated herein by reference for background purposes. In this technique random chemical mutagenesis of target organism, growth at temperature other than physiological temperature (permissive temperature) and subsequent replica plating and growth at different temperature (e.g. 42°C to identify ts, 25°C to identify cs) are used to identify those isolates which now fail to grow (conditional mutants). As above close monitoring of the changes upon growth at the non-permissive temperature yields information on the function of the mutated gene. Complementation of conditional lethal mutation by library from target organism and sequencing of complementing gene allows matching with unknown ORF.

6) RT-PCR: Streptococcus pneumoniae messenger RNA is isolated from bacterial infected tissue e.g. 48 hour murine lung infections, and the amount of each mRNA species assessed by reverse transcription of the RNA sample primed with random hexanucleotides

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SUBSTTTUTΈ SHEET (RULE 26) followed by PCR with gene specific primer pairs. The determination of the presence and amount of a particular mRNA species by quantification of the resultant PCR product provides information on the bacterial genes which are transcribed in the infected tissue. Analysis of gene transcription can be carried out at different times of infection to gain a detailed knowledge of gene regulation in bacterial pathogenesis allowing for a clearer understanding of which gene products represent targets for screens for novel antibacterials. Because of the gene specific nature of the PCR primers employed it should be understood that the bacterial mRNA preparation need not be free of mammalian RNA. This allows the investigator to carry out a simple and quick RNA preparation from infected tissue to obtain bacterial mRNA species which are very short lived in the bacterium (in the order of 2 minute halflives). Optimally the bacterial mRNA is prepared from infected murine lung tissue by mechanical disruption in the presence of TRIzole (GIBCO-BRL) for very short periods of time, subsequent processing according to the manufacturers of TRIzole reagent and DNAase treatment to remove contaminating DNA. Preferably the process is optimised by finding those conditions which give a maximum amount of Streptococcus pneumoniae 16S ribosomal RNA as detected by probing Northerns with a suitably labelled sequence specific oligonucleotide probe. Typically a 5' dye labelled primer is used in each PCR primer pair in a PCR reaction which is terminated optimally between 8 and 25 cycles. The PCR products are separated on 6% polyacrylamide gels with detection and quantification using GeneScanner (manufactured by ABI).

Each of these techniques may have advantages or disadvantage depending on the particular application. The skilled artisan would choose the approach that is the most relevant with the particular end use in mind.

Use of the of these technologies when applied to the ORFs of the present invention enables identification of bacterial proteins expressed during infection, inhibitors of which would have utility in anti-bacterial therapy.

The invention relates to novel polypeptides and polynucleotides as described in greater detail below. In particular, the invention relates to polypeptides and polynucleotides of Streptococcus pneumoniae, which is related by amino acid sequence homology to known polypeptide as set forth in Table 1. The invention relates especially to compounds having the nucleotide and amino acid sequence selected from the group consisting of the sequences set

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SUBSTT UTΕ SHEET (RULE 26) out in Table 1, and to the nucleotide sequences of the DNA in the deposited strain and amino acid sequences encoded thereby.

Deposited materials

The deposit has been made under the terms of the Budapest Treaty on the International Recognition of the Deposit of Micro-organisms for Purposes of Patent Procedure. The strain will be irrevocably and without restriction or condition released to the public upon the issuance of a patent. The deposit is provided merely as convenience to those of skill in the art and is not an admission that a deposit is required for enablement, such as that required under 35 U.S.C. §112.

A deposit containing a Streptococcus pneumoniae bacterial strain has been deposited with the National Collections of Industrial and Marine Bacteria Ltd. (NCIMB), 23 St. Machar Drive, Aberdeen AB2 1RY, Scotland on 11 April 1996 and assigned NCIMB Deposit No. 40794. The Streptococcus pneumoniae bacterial strain deposit is referred to herein as "the deposited bacterial strain" or as "the DNA of the deposited bacterial strain."

The deposited material is a bacterial strain that contains the full length FabH DNA, referred to as "NCIMB 40794" upon deposit.

The sequence of the polynucleotides contained in the deposited material, as well as the amino acid sequence of the polypeptide encoded thereby, are controlling in the event of any conflict with any description of sequences herein.

A license may be required to make, use or sell the deposited materials, and no such license is hereby granted.

The deposited strain contains the full length genes comprising the polynucleotides set forth in Table 1. The sequence of the polynucleotides contained in the deposited strain, as well as the amino acid sequence of the polypeptide encoded thereby, are controlling in the event of any conflict with any description of sequences herein.

Polypeptides

The polypeptides of the invention include the polypeptides set forth in Table 1 (in particular the mature polypeptide) as well as polypeptides and fragments, particularly those which have the biological activity of a polypeptide of the invention, and also those which have at least 50%, 60% or 70% identity to a polypeptide sequence selected from the group consisting of the sequences set out in Table 1 or the relevant portion, preferably at least 80% identity to a polypeptide sequence selected from the group consisting of the sequences set out in Table 1, and more preferably at least 90% similarity (more preferably at least 90% identity) to a polypeptide sequence selected from the group consisting of the sequences set out in Table 1, and still more preferably at least 95% similarity (still more preferably at least 95% identity) to a polypeptide sequence selected from the group consisting of the sequences set out in Table 1, and also include portions of such polypeptides with such portion of the polypeptide generally containing at least 30 amino acids and more preferably at least 50 amino acids.

The invention also includes polypeptides of the formula:

X-(R_])_n-(R₂)-(R₃)_n-Y wherein, at the amino terminus, X is hydrogen, and at the carboxyl terminus, Y is hydrogen or a metal, Ri and R3 are any amino acid residue, n is an integer between 1 and 2000, and R₂ is an amino acid sequence of the invention, particularly an amino acid sequence selected from the group set forth in Table 1. In the formula above R₂ is oriented so that its amino terminal residue is at the left, bound to R1 and its carboxy terminal residue is at the right, bound to R3. Any stretch of amino acid residues denoted by either R group, where R is greater than 1 , may be either a heteropolymer or a homopolymer, preferably a heteropolymer. In preferred embodiments n is an integer between 1 and 1000 or 2000.

A fragment is a variant polypeptide having an amino acid sequence that entirely is the same as part but not all of the amino acid sequence of the aforementioned polypeptides. As with polypeptides, fragments may be "free-standing," or comprised within a larger polypeptide of which they form a part or region, most preferably as a single continuous region, a single larger polypeptide.

Preferred fragments include, for example, truncation polypeptides having a portion of the amino acid sequence of Table 1 , or of variants thereof, such as a continuous series of residues that includes the amino terminus, or a continuous series of residues that includes the carboxyl terminus. Degradation forms of the polypeptides of the invention in a host cell, particularly a Streptococcus pneumoniae, are also preferred. Further preferred are fragments characterized by structural or functional attributes such as fragments that comprise alpha-helix and alpha-helix forming regions, beta-sheet and beta-sheet-forming regions, turn and turn- forming regions, coil and coil-forming regions, hydrophilic regions, hydrophobic regions, alpha amphipathic regions, beta amphipathic regions, flexible regions, surface-forming regions, substrate binding region, and high antigenic index regions.

Also preferred are biologically active fragments which are those fragments that mediate activities of polypeptides of the invention, including those with a similar activity or an improved activity, or with a decreased undesirable activity. Also included are those

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SUBSTTTUTΕ SHEET (RULE 26) fragments that are antigenic or immunogenic in an animal, especially in a human. Particularly preferred are fragments comprising receptors or domains of enzymes that confer a function essential for viability of Streptococcus pneumoniae or the ability to initiate, or maintain cause disease in an individual, particularly a human.

Variants that are fragments of the polypeptides of the invention may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, these variants may be employed as intermediates for producing the full-length polypeptides of the invention.

In addition to the standard single and triple letter representations for amino acids, the term "X" or "Xaa" is also used. "X" and "Xaa" mean that any of the twenty naturally occuring amino acids may appear at such a designated position in the polypeptide sequence.

Polynucleotides

The nucleotide sequences disclosed herein can be obtained by synthetic chemical techniques known in the art or can be obtained from S. pneumoniae 0100993 by probing a DNA preparation with probes constructed from the particular sequences disclosed herein. Alternatively, oligonucleotides derived from a disclosed sequence can act as PCR primers in a process of PCR-based cloning of the sequence from a bacterial genomic source. It is recognised that such sequences will also have utility in diagnosis of the stage of infection and type of infection the pathogen has attained.

To obtain the polynucleotide encoding the protein using the DNA sequence given herein typically a library of clones of chromosomal DNA of S. pneumoniae 0100993 in E. coli or some other suitable host is probed with a radiolabelled oligonucleotide, preferably a 17mer or longer, derived from the partial sequence. Clones carrying DNA identical to that of the probe can then be distinguished using high stringency washes. By sequencing the individual clones thus identified with sequencing primers designed from the original sequence it is then possible to extend the sequence in both directions to determine the full gene sequence. Conveniently such sequencing is performed using denatured double stranded DNA prepared from a plasmid clone. Suitable techniques are described by Maniatis, T., Fritsch, E.F. and Sambrook, J. in MOLECULAR CLONING, A Laboratory Manual, 2nd edition, 1989, Cold Spring Harbor Laboratory (see: Screening By Hybridization 1.90 and Sequencing Denatured Double-Stranded DNA Templates 13.70).

Moerover, another aspect of the invention relates to isolated polynucleotides that encode the polypeptides of the invention having a deduced amino acid sequence selected from

16

SUBSTT UTE SHEET (RULE 26) the group consisting of the sequences in Table 1 and polynucleotides closely related thereto and variants thereof.

Using the information provided herein, such as the polynucleotide sequences set out in Table 1, a polynucleotide of the invention encoding polypeptide may be obtained using standard cloning and screening methods, such as those for cloning and sequencing chromosomal DNA fragments from bacteria using Streptococcus pneumoniae 0100993 cells as starting material, followed by obtaining a full length clone. For example, to obtain a polynucleotide sequence of the invention, such as a sequence set forth in Table 1 , typically a library of clones of chromosomal DNA of Streptococcus pneumoniae 0100993 in E.coli or some other suitable host is probed with a radiolabeled oligonucleotide, preferably a 17- mer or longer, derived from a partial sequence. Clones carrying DNA identical to that of the probe can then be distinguished using stringent conditions. By sequencing the individual clones thus identified with sequencing primers designed from the original sequence it is then possible to extend the sequence in both directions to determine the full gene sequence. Conveniently, such sequencing is performed using denatured double stranded DNA prepared from a plasmid clone. Suitable techniques are described by Maniatis, T., Fritsch, E.F. and Sambrook et al., MOLECULAR CLONING, A LABORATORY MANUAL, 2nd Ed.; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989). (see in particular Screening By Hybridization 1.90 and Sequencing Denatured Double-Stranded DNA Templates 13.70). Illustrative of the invention, the polynucleotides set out in Table 1 were discovered in a DNA library derived from Streptococcus pneumoniae 0100993.

The DNA sequences set out in Table 1 each contains at least one open reading frame encoding a protein having at least about the number of amino acid residues set forth in Table 1. The start and stop codons of each open reading frame (herein "ORF") DNA are the first three and the last three nuclotides of each polynucleotide set forth in Table 1.

Certain polynucleotides and polypeptides of the invention are structurally related to known proteins as set forth in Table 1. These proteins exhibit greatest homology to the homologue listed in Table 1 from among the known proteins.

The invention provides a polynucleotide sequence identical over its entire length to each coding sequence in Table 1. Also provided by the invention is the coding sequence for the mature polypeptide or a fragment thereof, by itself as well as the coding sequence for the mature polypeptide or a fragment in reading frame with other coding sequence, such as those

17

SUBSTTTUTE SHEET (RULE 26) encoding a leader or secretory sequence, a pre-, or pro- or prepro- protein sequence. The polynucleotide may also contain non-coding sequences, including for example, but not limited to non-coding 5' and 3' sequences, such as the transcribed, non-translated sequences, termination signals, ribosome binding sites, sequences that stabilize mRNA, introns, polyadenylation signals, and additional coding sequence which encode additional amino acids. For example, a marker sequence that facilitates purification of the fused polypeptide can be encoded. In certain embodiments of the invention, the marker sequence is a hexa- histidine peptide, as provided in the pQE vector (Qiagen, Inc.) and described in Gentz et al, Proc. Natl Acad. Sci, USA 86: 821-824 (1989), or an HA tag (Wilson et al, Cell 37: 767 (1984). Polynucleotides of the invention also include, but are not limited to, polynucleotides comprising a structural gene and its naturally associated sequences that control gene expression.

The invention also includes polynucleotides of the formula:

X-(R₁)_n-(R₂)-(R₃)_n-Y wherein, at the 5' end of the molecule, X is hydrogen, and at the 3' end of the molecule, Y is hydrogen or a metal, Rj and R3 is any nucleic acid residue, n is an integer between 1 and 3000, and R₂ is a nucleic acid sequence of the invention, particularly a nucleic acid sequence selected from the group set forth in Table 1. In the polynucleotide formula above R₂ is oriented so that its 5' end residue is at the left, bound to R1 and its 3' end residue is at the right, bound to R3. Any stretch of nucleic acid residues denoted by either R group, where R is greater than 1 , may be either a heteropolymer or a homopolymer, preferably a heteropolymer. In a preferred embodiment n is an integer between 1 and 1000, or 2000 or 3000.

The term "polynucleotide encoding a polypeptide" as used herein encompasses polynucleotides that include a sequence encoding a polypeptide of the invention, particularly a bacterial polypeptide and more particularly a polypeptide of the Streptococcus pneumoniae having an amino acid sequence set out in Table 1. The term also encompasses polynucleotides that include a single continuous region or discontinuous regions encoding the polypeptide (for example, interrupted by integrated phage or an insertion sequence or editing) together with additional regions, that also may contain coding and/or non-coding sequences.

The invention further relates to variants of the polynucleotides described herein that encode for variants of the polypeptide having the deduced amino acid sequence of Table 1. Variants that are fragments of the polynucleotides of the invention may be used to synthesize full-length polynucleotides of the invention.

18

SUBSTTTUTE SHEET (RULE 26) Further particularly preferred embodiments are polynucleotides encoding polypeptide variants, that have the amino acid sequence of a polypeptide of Table 1 in which several, a few, 5 to 10, 1 to 5, 1 to 3, 2, 1 or no amino acid residues are substituted, deleted or added, in any combination. Especially preferred among these are silent substitutions, additions and deletions, that do not alter the properties and activities of such polynucleotide.

Further preferred embodiments of the invention are polynucleotides that are at least 50%, 60% or 70% identical over their entire length to a polynucleotide encoding a polypeptide having the amino acid sequence set out in Table 1, and polynucleotides that are complementary to such polynucleotides. Alternatively, most highly preferred are polynucleotides that comprise a region that is at least 80% identical over its entire length to a polynucleotide encoding a polypeptide of the deposited strain and polynucleotides complementary thereto. In this regard, polynucleotides at least 90% identical over their entire length to the same are particularly preferred, and among these particularly preferred polynucleotides. those with at least 95% are especially preferred. Furthermore, those with at least 97% are highly preferred among those with at least 95%, and among these those with at least 98% and at least 99% are particularly highly preferred, with at least 99% being the more preferred.

A preferred embodiment is an isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of: a polynucleotide having at least a 50% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of Table 1 and obtained from a prokaryotic species other than S. pneumoniae; and a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 50% identical to the amino acid sequence of Table 1 and obtained from a prokaryotic species other than S. pneumoniae.

Preferred embodiments are polynucleotides that encode polypeptides that retain substantially the same biological function or activity as the mature polypeptide encoded by the DNA of Table 1.

The invention further relates to polynucleotides that hybridize to the herein above- described sequences. In this regard, the invention especially relates to polynucleotides that hybridize under stringent conditions to the herein above-described polynucleotides. As herein used, the terms "stringent conditions" and "stringent hybridization conditions" mean hybridization will occur only if there is at least 95% and preferably at least 97% identity between the sequences. An example of stringent hybridization conditions is overnight

19

SUBSTTTUTE SHEET (RULE 26) incubation at 42°C in a solution comprising: 50% formamide, 5x SSC (150mM NaCl, 15mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5x Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing the hybridization support in O.lx SSC at about 65°C. Hybridization and wash conditions are well known and exemplified in Sambrook, et al, Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., (1989), particularly Chapter 11 therein.

The invention also provides a polynucleotide consisting essentially of a polynucleotide sequence obtainable by screening an appropriate library containing the complete gene for a polynucleotide sequence set forth in Table 1 under stringent hybridization conditions with a probe having the sequence of said polynucleotide sequence or a fragment thereof; and isolating said DNA sequence. Fragments useful for obtaining such a polynucleotide include, for example, probes and primers described elsewhere herein.

As discussed additionally herein regarding polynucleotide assays of the invention, for instance, polynucleotides of the invention as discussed above, may be used as a hybridization probe for RNA, cDNA and genomic DNA to isolate full-length cDNAs and genomic clones encoding a polypeptide and to isolate cDNA and genomic clones of other genes that have a high sequence similarity to a polynucleotide set forth in Table 1. Such probes generally will comprise at least 15 bases. Preferably, such probes will have at least 30 bases and may have at least 50 bases. Particularly preferred probes will have at least 30 bases and will have 50 bases or less.

For example, the coding region of each gene that comprises or is comprised by a polynucleotide set forth in Table 1 may be isolated by screening using a DNA sequence provided in Table 1 to synthesize an oligonucleotide probe. A labeled oligonucleotide having a sequence complementary to that of a gene of the invention is then used to screen a library of cDNA, genomic DNA or mRNA to determine which members of the library the probe hybridizes to.

The polynucleotides and polypeptides of the invention may be employed, for example, as research reagents and materials for discovery of treatments of and diagnostics for disease, particularly human disease, as further discussed herein relating to polynucleotide assays.

Polynucleotides of the invention that are oligonucleotides derived from the a polynucleotide or polypeptide sequence set forth in Table 1 may be used in the processes

20

SUBSTTTUTΕ SHEET (RULE 26) herein as described, but preferably for PCR, to determine whether or not the polynucleotides identified herein in whole or in part are transcribed in bacteria in infected tissue. It is recognized that such sequences will also have utility in diagnosis of the stage of infection and type of infection the pathogen has attained.

The invention also provides polynucleotides that may encode a polypeptide that is the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature polypeptide (when the mature form has more than one polypeptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, may allow protein transport, may lengthen or shorten protein half-life or may facilitate manipulation of a protein for assay or production, among other things. As generally is the case in vivo, the additional amino acids may be processed away from the mature protein by cellular enzymes.

A precursor protein, having the mature form of the polypeptide fused to one or more prosequences may be an inactive form of the polypeptide. When prosequences are removed such inactive precursors generally are activated. Some or all of the prosequences may be removed before activation. Generally, such precursors are called proproteins.

In addition to the standard A, G, C, T/U representations for nucleic acid bases, the term "N" is also used. "N" means that any of the four DNA or RNA bases may appear at such a designated position in the DNA or RNA sequence, except it is preferred that N is not a base that when taken in combination with adjacent nucleotide positions, when read in the correct reading frame, would have the effect of generating a premature termination codon in such reading frame.

In sum, a polynucleotide of the invention may encode a mature protein, a mature protein plus a leader sequence (which may be referred to as a preprotein), a precursor of a mature protein having one or more prosequences that are not the leader sequences of a preprotein, or a preproprotein, which is a precursor to a proprotein, having a leader sequence and one or more prosequences, which generally are removed during processing steps that produce active and mature forms of the polypeptide.

Vectors, host cells, expression

The invention also relates to vectors that comprise a polynucleotide or polynucleotides of the invention, host cells that are genetically engineered with vectors of the invention and the production of polypeptides of the invention by recombinant techniques.

21

SUBSTTTUTE SHEET (RULE 26) Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the invention.

For recombinant production, host cells can be genetically engineered to incorporate expression systems or portions thereof or polynucleotides of the invention. Introduction of a polynucleotide into the host cell can be effected by methods described in many standard laboratory manuals, such as Davis et al, BASIC METHODS IN MOLECULAR BIOLOGY, (1986) and Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989), such as, calcium phosphate transfection, DEAE-dextran mediated transfection, transvection, microinjection, cationic lipid-mediated transfection, electroporation, transduction, scrape loading, ballistic introduction and infection.

Representative examples of appropriate hosts include bacterial cells, such as streptococci, staphylococci, enterococci E. coli, streptomyces and Bacillus subtilis cells; fungal cells, such as yeast cells and Aspergillus cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS, HeLa, C127, 3T3, BHK, 293 and Bowes melanoma cells; and plant cells.

A great variety of expression systems can be used to produce the polypeptides of the invention. Such vectors include, among others, chromosomal, episomal and virus-derived vectors, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof, such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression system constructs may contain control regions that regulate as well as engender expression. Generally, any system or vector suitable to maintain, propagate or express polynucleotides and/or to express a polypeptide in a host may be used for expression in this regard. The appropriate DNA sequence may be inserted into the expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et al, MOLECULAR CLONING, A LABORATORY MANUAL, (supra).

For secretion of the translated protein into the lumen of the endoplasmic reticulum, into the periplasmic space or into the extracellular environment, appropriate secretion signals

22

SUBSTTTUTE SHEET (RULE 26) may be incorporated into the expressed polypeptide. These signals may be endogenous to the polypeptide or they may be heterologous signals.

Polypeptides of the invention can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography, and lectin chromatography. Most preferably, high performance liquid chromatography is employed for purification. Well known techniques for refolding protein may be employed to regenerate active conformation when the polypeptide is denatured during isolation and or purification.

Diagnostic Assays

This invention is also related to the use of the polynucleotides of the invention for use as diagnostic reagents. Detection of such polynucleotides in a eukaryote, particularly a mammal, and especially a human, will provide a diagnostic method for diagnosis of a disease. Eukaryotes (herein also "individual(s)"), particularly mammals, and especially humans, infected with an organism comprising a gene of the invention may be detected at the nucleic acid level by a variety of techniques.

Nucleic acids for diagnosis may be obtained from an infected individual's cells and tissues, such as bone, blood, muscle, cartilage, and skin. Genomic DNA may be used directly for detection or may be amplified enzymatically by using PCR or other amplification technique prior to analysis. RNA or cDNA may also be used in the same ways. Using amplification, characterization of the species and strain of prokaryote present in an individual, may be made by an analysis of the genotype of the prokaryote gene. Deletions and insertions can be detected by a change in size of the amplified product in comparison to the genotype of a reference sequence. Point mutations can be identified by hybridizing amplified DNA to labeled polynucleotide sequences of the invention. Perfectly matched sequences can be distinguished from mismatched duplexes by RNase digestion or by differences in melting temperatures. DNA sequence differences may also be detected by alterations in the electrophoretic mobility of the DNA fragments in gels, with or without denaturing agents, or by direct DNA sequencing. See, e.g., Myers et al., Science, 230: 1242 (1985). Sequence changes at specific locations also may be revealed by nuclease protection assays, such as RNase and SI protection or a chemical cleavage method. See, e.g., Cotton et al., Proc. Natl. Acad. Set, USA, 85: 4397-4401

23

SUBSTTTUTE SHEET (RULE 26) (1985).

Cells carrying mutations or polymorphisms in the gene of the invention may also be detected at the DNA level by a variety of techniques, to allow for serotyping, for example. For example, RT-PCR can be used to detect mutations. It is particularly preferred to used RT- PCR in conjunction with automated detection systems, such as, for example, GeneScan. RNA or cDNA may also be used for the same purpose, PCR or RT-PCR. As an example, PCR primers complementary to a nucleic acid encoding a polypeptide of the invention can be used to identify and analyze mutations. These primers may be used for, among other things, amplifying a DNA of the invention isolated from a sample derived from an individual. The primers may be used to amplify the gene isolated from an infected individual such that the gene may then be subject to various techniques for elucidation of the DNA sequence. In this way, mutations in the DNA sequence may be detected and used to diagnose infection and to serotype and/or classify the infectious agent.

The invention further provides a process for diagnosing disease, preferably bacterial infections, more preferably infections by Streptococcus pneumoniae, and most preferably disease, comprising determining from a sample derived from an individual a increased level of expression of polynucleotide having the sequence of Table 1. Increased or decreased expression of a polynucleotide of the invention can be measured using any on of the methods well known in the art for the quantitation of polynucleotides, such as, for example, amplification, PCR, RT-PCR, RNase protection, Northern blotting and other hybridization methods.

In addition, a diagnostic assay in accordance with the invention for detecting over- expression of a polypeptide of the invention compared to normal control tissue samples may be used to detect the presence of an infection, for example. Assay techniques that can be used to determine levels of a protein, in a sample derived from a host are well-known to those of skill in the art. Such assay methods include radioimmunoassays, competitive-binding assays, Western Blot analysis and ELISA assays.

Antibodies

The polypeptides of the invention or variants thereof, or cells expressing them can be used as an immunogen to produce antibodies immunospecific for such polypeptides. "Antibodies" as used herein includes monoclonal and polyclonal antibodies, chimeric, single chain, simianized antibodies and humanized antibodies, as well as Fab fragments, including the products of an Fab immunolglobulin expression library.

24

SUBSTTTUTE SHEET (RULE 26) Antibodies generated against the polypeptides of the invention can be obtained by administering the polypeptides or epitope-bearing fragments, analogues or cells to an animal, preferably a nonhuman, using routine protocols. For preparation of monoclonal antibodies, any technique known in the art that provides antibodies produced by continuous cell line cultures can be used. Examples include various techniques, such as those in Kohler, G. and Milstein, C, Nature 256: 495-497 (1975); Kozbor et al, Immunology Today 4: 72 (1983); Cole et al., pg. 77-96 in MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc. (1985).

Techniques for the production of single chain antibodies (U.S. Patent No. 4,946,778) can be adapted to produce single chain antibodies to polypeptides of this invention. Also, transgenic mice, or other organisms such as other mammals, may be used to express humanized antibodies.

Alternatively phage display technology may be utilized to select antibody genes with binding activities towards the polypeptide either from repertoires of PCR amplified v- genes of lymphocytes from humans screened for possessing recognition of a polypeptide of the invention or from naive libraries (McCafferty, J. et al., (1990), Nature 348, 552-554; Marks, J. et al, (1992) Biotechnology 10, 779-783). The affinity of these antibodies can also be improved by chain shuffling (Clackson, T. et al., (1991) Nature 352, 624-628).

If two antigen binding domains are present each domain may be directed against a different epitope - termed 'bispecific' antibodies.

The above-described antibodies may be employed to isolate or to identify clones expressing the polypeptides to purify the polypeptides by affinity chromatography.

Thus, among others, antibodies against a polypeptide of the invention may be employed to treat disease.

Polypeptide variants include antigenically, epitopically or immunologically equivalent variants that form a particular aspect of this invention. The term "antigenically equivalent derivative" as used herein encompasses a polypeptide or its equivalent which will be specifically recognized by certain antibodies which, when raised to the protein or polypeptide according to the invention, interfere with the immediate physical interaction between pathogen and mammalian host. The term "immunologically equivalent derivative" as used herein encompasses a peptide or its equivalent which when used in a suitable formulation to raise antibodies in a vertebrate, the antibodies act to interfere with the immediate physical interaction between pathogen and mammalian host.

25

SUBSTTTUTE SHEET (RULE 26) The polypeptide, such as an antigenically or immunologically equivalent derivative or a fusion protein thereof is used as an antigen to immunize a mouse or other animal such as a rat or chicken. The fusion protein may provide stability to the polypeptide. The antigen may be associated, for example by conjugation, with an immunogenic carrier protein for example bovine serum albumin (BSA) or keyhole limpet haemocyanin (KLH). Alternatively a multiple antigenic peptide comprising multiple copies of the protein or polypeptide, or an antigenically or immunologically equivalent polypeptide thereof may be sufficiently antigenic to improve immunogenicity so as to obviate the use of a carrier.

Preferably, the antibody or variant thereof is modified to make it less immunogenic in the individual. For example, if the individual is human the antibody may most preferably be "humanized"; where the complimentarity determining region(s) of the hybridoma-derived antibody has been transplanted into a human monoclonal antibody , for example as described in Jones, P. et al. (1986), Nature 321, 522-525 or Tempest et al.,(1991) Biotechnology 9, 266-273.

The use of a polynucleotide of the invention in genetic immunization will preferably employ a suitable delivery method such as direct injection of plasmid DNA into muscles (Wolff et al., Hum Mol Genet 1992, 1:363, Manthorpe et al., Hum. Gene Ther. 1963:4, 419), delivery of DNA complexed with specific protein carriers (Wu et al., J Biol Chem. 1989: 264,16985), coprecipitation of DNA with calcium phosphate (Benvenisty & Reshef, PNAS, 1986:83,9551), encapsulation of DNA in various forms of liposomes (Kaneda et al, Science 1989:243,375), particle bombardment (Tang et al., Nature 1992, 356: 152, Eisenbraun et al., DNA Cell Biol 1993, 12:791) and in vivo infection using cloned retroviral vectors (Seeger et al., PNAS 1984:81,5849).

Antagonists and agonists - assays and molecules

Polypeptides of the invention may also be used to assess the binding of small molecule substrates and ligands in, for example, cells, cell-free preparations, chemical libraries, and natural product mixtures. These substrates and ligands may be natural substrates and ligands or may be structural or functional mimetics. See, e.g., Coligan et al, Current Protocols in Immunology 1(2): Chapter 5 (1991).

The invention also provides a method of screening compounds to identify those which enhance (agonist) or block (antagonist) the action of a polypeptides or polynucleotides of the invention, particularly those compounds that are bacteriostatic and/or bacteriocidal. The method of screening may involve high-throughput techniques. For example, to screen for

26

SUBSTTTUTE SHEET (RULE 26) agonists or antagoists, a synthetic reaction mix, a cellular compartment, such as a membrane, cell envelope or cell wall, or a preparation of any thereof, comprising a polypeptide of the invention and a labeled substrate or ligand of such polypeptide is incubated in the absence or the presence of a candidate molecule that may be an agonist or antagonist of a polypeptide of the invention. The ability of the candidate molecule to agonize or antagonize a polypeptide of the invention is reflected in decreased binding of the labeled ligand or decreased production of product from such substrate. Molecules that bind gratuitously, i.e., without inducing the effects of a polypeptide of the invention are most likely to be good antagonists. Molecules that bind well and increase the rate of product production from substrate are agonists. Detection of the rate or level of production of product from substrate may be enhanced by using a reporter system. Reporter systems that may be useful in this regard include but are not limited to colorimetric labeled substrate converted into product, a reporter gene that is responsive to changes in polynucleotide or polypeptide activity, and binding assays known in the art.

Another example of an assay for antagonists of polypeptides of the invention is a competitive assay that combines any such polypeptide and a potential antagonist with a compound which binds such polypeptide, natural substrates or ligands, or substrate or ligand mimetics, under appropriate conditions for a competitive inhibition assay. A polypeptide of the invention can be labeled, such as by radioactivity or a colorimetric compound, such that the number of such polypeptide molecules bound to a binding molecule or converted to product can be determined accurately to assess the effectiveness of the potential antagonist.

Potential antagonists include small organic molecules, peptides, polypeptides and antibodies that bind to a polynucleotide or polypeptide of the invention and thereby inhibit or extinguish its activity. Potential antagonists also may be small organic molecules, a peptide, a polypeptide such as a closely related protein or antibody that binds the same sites on a binding molecule, such as a binding molecule, without inducing activities induced by a polypeptide of the invention, thereby preventing the action of such polypeptide by excluding it from binding.

Potential antagonists include a small molecule that binds to and occupies the binding site of the polypeptide thereby preventing binding to cellular binding molecules, such that normal biological activity is prevented. Examples of small molecules include but are not limited to small organic molecules, peptides or peptide-like molecules. Other potential antagonists include antisense molecules (see Okano, J. Neurochem. 56: 560 (1991); OLIGODEOXYNUCLEOTIDES AS ANTISENSE INHIBITORS OF GENE EXPRESSION,

27

SUBSTTTUTE SHEET (RULE 26) CRC Press, Boca Raton, FL (1988), for a description of these molecules). Preferred potential antagonists include compounds related to and variants of a polypeptide of the invention.

Each of the DNA sequences provided herein may be used in the discovery and development of antibacterial compounds. The encoded protein, upon expression, can be used as a target for the screening of antibacterial drugs. Additionally, the DNA sequences encoding the amino terminal regions of the encoded protein or Shine-Delgarno or other translation facilitating sequences of the respective mRNA can be used to construct antisense sequences to control the expression of the coding sequence of interest.

The invention also provides the use of the polypeptide, polynucleotide or inhibitor of the invention to interfere with the initial physical interaction between a pathogen and mammalian host responsible for sequelae of infection. In particular the molecules of the invention may be used: in the prevention of adhesion of bacteria, in particular gram positive bacteria, to mammalian extracellular matrix proteins on in-dwelling devices or to extracellular matrix proteins in wounds; to block protein-mediated mammalian cell invasion by, for example, initiating phosphorylation of mammalian tyrosine kinases (Rosenshine et al, Infect. Immun. 60:2211 (1992); to block bacterial adhesion between mammalian extracellular matrix proteins and bacterial proteins that mediate tissue damage and; to block the normal progression of pathogenesis in infections initiated other than by the implantation of in-dwelling devices or by other surgical techniques.

The antagonists and agonists of the invention may be employed, for instance, to inhibit and treat disease.

Helicobacter pylori (herein H. pylori) bacteria infect the stomachs of over one-third of the world's population causing stomach cancer, ulcers, and gastritis (International Agency for Research on Cancer (1994) Schistosomes, Liver Flukes and Helicobacter Pylori (International Agency for Research on Cancer, Lyon, France; http://www.uicc.ch/ecp/ecp2904.htm). Moreover, the international Agency for Research on Cancer recently recognized a cause-and-effect relationship between H. pylori and gastric adenocarcinoma, classifying the bacterium as a Group I (definite) carcinogen. Preferred antimicrobial compounds of the invention found using screens provided by the invention, particularly broad-spectrum antibiotics, should be useful in the treatment of H. pylori infection. Such treatment should decrease the advent of H. pylori-induced cancers, such as gastrointestinal carcinoma. Such treatment should also cure gastric ulcers and gastritis.

Vaccines

28

SUBSTTTUTE SHEET (RULE 26) Another aspect of the invention relates to a method for inducing an immunological response in an individual, particularly a mammal which comprises inoculating the individual with a polypeptide of the invention, or a fragment or variant thereof, adequate to • produce antibody and/ or T cell immune response to protect said individual from infection, particularly bacterial infection and most particularly Streptococcus pneumoniae infection. Also provided are methods whereby such immunological response slows bacterial replication. Yet another aspect of the invention relates to a method of inducing immunological response in an individual which comprises delivering to such individual a nucleic acid vector to direct expression of a polynucleotide or polypeptide of the invention, or a fragment or a variant thereof, for expressing such polynucleotide or polypeptide, or a fragment or a variant thereof in vivo in order to induce an immunological response, such as, to produce antibody and/ or T cell immune response, including, for example, cytokine- producing T cells or cytotoxic T cells, to protect said individual from disease, whether that disease is already established within the individual or not. One way of administering the gene is by accelerating it into the desired cells as a coating on particles or otherwise. Such nucleic acid vector may comprise DNA, RNA, a modified nucleic acid, or a DNA/RNA hybrid.

A further aspect of the invention relates to an immunological composition which, when introduced into an individual capable or having induced within it an immunological response, induces an immunological response in such individual to a polynucleotide of the invention or protein coded therefrom, wherein the composition comprises a recombinant polynucleotide or protein coded therefrom comprising DNA which codes for and expresses an antigen of said polynucleotide or protein coded therefrom. The immunological response may be used therapeutically or prophylactically and may take the form of antibody immunity or cellular immunity such as that arising from CTL or CD4+ T cells.

A polypeptide of the invention or a fragment thereof may be fused with co-protein which may not by itself produce antibodies, but is capable of stabilizing the first protein and producing a fused protein which will have immunogenic and protective properties. Thus fused recombinant protein, preferably further comprises an antigenic co-protein, such as lipoprotein D from Hemophilus influenzae, Glutathione-S-transferase (GST) or beta- galactosidase, relatively large co-proteins which solubilize the protein and facilitate production and purification thereof. Moreover, the co-protein may act as an adjuvant in the sense of providing a generalized stimulation of the immune system. The co-protein may be attached to either the amino or carboxy terminus of the first protein.

Provided by this invention are compositions, particularly vaccine compositions, and methods comprising the polypeptides or polynucleotides of the invention and immunostimulatory DNA sequences, such as those described in Sato, Y. et al. Science 273: 352 (1996).

Also, provided by this invention are methods using the described polynucleotide or particular fragments thereof which have been shown to encode non-variable regions of bacterial cell surface proteins in DNA constructs used in such genetic immunization experiments in animal models of infection with Streptococcus pneumoniae will be particularly useful for identifying protein epitopes able to provoke a prophylactic or therapeutic immune response. It is believed that this approach will allow for the subsequent preparation of monoclonal antibodies of particular value from the requisite organ of the animal successfully resisting or clearing infection for the development of prophylactic agents or therapeutic treatments of bacterial infection, particularly Streptococcus pneumoniae infection, in mammals, particularly humans.

The polypeptide may be used as an antigen for vaccination of a host to produce specific antibodies which protect against invasion of bacteria, for example by blocking adherence of bacteria to damaged tissue. Examples of tissue damage include wounds in skin or connective tissue caused, e.g., by mechanical, chemical or thermal damage or by implantation of indwelling devices, or wounds in the mucous membranes, such as the mouth, mammary glands, urethra or vagina.

The invention also includes a vaccine formulation which comprises an immunogenic recombinant protein of the invention together with a suitable carrier. Since the protein may be broken down in the stomach, it is preferably administered parenterally, including, for example, administration that is subcutaneous, intramuscular, intravenous, or intradermal. Formulations suitable for parenteral administration include aqueous and non- aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the bodily fluid, preferably the blood, of the individual; and aqueous and non-aqueous sterile suspensions which may include suspending agents or thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampules and vials and may be stored in a freeze-dried condition requiring only the addition of the sterile liquid carrier

30

SUBSTTTUTE SHEET (RULE 26) immediately prior to use. The vaccine formulation may also include adjuvant systems for enhancing the immunogenicity of the formulation, such as oil-in water systems and other systems known in the art. The dosage will depend on the specific activity of the vaccine and can be readily determined by routine experimentation.

While the invention has been described with reference to certain protein, such as, for example, those set forth in Table 1 , it is to be understood that this covers fragments of the naturally occurring protein and similar proteins with additions, deletions or substitutions which do not substantially affect the immunogenic properties of the recombinant protein.

Compositions, kits and administration

The invention also relates to compositions comprising the polynucleotide or the polypeptides discussed above or their agonists or antagonists. The polypeptides of the invention may be employed in combination with a non-sterile or sterile carrier or carriers for use with cells, tissues or organisms, such as a pharmaceutical carrier suitable for administration to a subject. Such compositions comprise, for instance, a media additive or a therapeutically effective amount of a polypeptide of the invention and a pharmaceutically acceptable carrier or excipient. Such carriers may include, but are not limited to, saline, buffered saline, dextrose, water, glycerol, ethanol and combinations thereof. The formulation should suit the mode of administration. The invention further relates to diagnostic and pharmaceutical packs and kits comprising one or more containers filled with one or more of the ingredients of the aforementioned compositions of the invention.

Polypeptides and other compounds of the invention may be employed alone or in conjunction with other compounds, such as therapeutic compounds.

The pharmaceutical compositions may be administered in any effective, convenient manner including, for instance, administration by topical, oral, anal, vaginal, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal or intradermal routes among others.

In therapy or as a prophylactic, the active agent may be administered to an individual as an injectable composition, for example as a sterile aqueous dispersion, preferably isotonic.

Alternatively the composition may be formulated for topical application for example in the form of ointments, creams, lotions, eye ointments, eye drops, ear drops, mouthwash, impregnated dressings and sutures and aerosols, and may contain appropriate conventional additives, including, for example, preservatives, solvents to assist drug

31

SUBSTTTUTE SHEET (RULE 26) penetration, and emollients in ointments and creams. Such topical formulations may also contain compatible conventional carriers, for example cream or ointment bases, and ethanol or oleyl alcohol for lotions. Such carriers may constitute from about 1% to about 98% by weight of the formulation; more usually they will constitute up to about 80% by weight of the formulation.

For administration to mammals, and particularly humans, it is expected that the daily dosage level of the active agent will be from 0.01 mg/kg to 10 mg/kg, typically around 1 mg/kg. The physician in any event will determine the actual dosage which will be most suitable for an individual and will vary with the age, weight and response of the particular individual. The above dosages are exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

In-dwelling devices include surgical implants, prosthetic devices and catheters, i.e., devices that are introduced to the body of an individual and remain in position for an extended time. Such devices include, for example, artificial joints, heart valves, pacemakers, vascular grafts, vascular catheters, cerebrospinal fluid shunts, urinary catheters, continuous ambulatory peritoneal dialysis (CAPD) catheters.

The composition of the invention may be administered by injection to achieve a systemic effect against relevant bacteria shortly before insertion of an in-dwelling device. Treatment may be continued after surgery during the in-body time of the device. In addition, the composition could also be used to broaden perioperative cover for any surgical technique to prevent bacterial wound infections, especially Streptococcus pneumoniae wound infections.

Many orthopedic surgeons consider that humans with prosthetic joints should be considered for antibiotic prophylaxis before dental treatment that could produce a bacteremia. Late deep infection is a serious complication sometimes leading to loss of the prosthetic joint and is accompanied by significant morbidity and mortality. It may therefore be possible to extend the use of the active agent as a replacement for prophylactic antibiotics in this situation.

In addition to the therapy described above, the compositions of this invention may be used generally as a wound treatment agent to prevent adhesion of bacteria to matrix proteins exposed in wound tissue and for prophylactic use in dental treatment as an alternative to, or in conjunction with, antibiotic prophylaxis. Alternatively, the composition of the invention may be used to bathe an indwelling device immediately before insertion. The active agent will preferably be present at a concentration of lμg/ml to lOmg/ml for bathing of wounds or indwelling devices.

A vaccine composition is conveniently in injectable form. Conventional adjuvants may be employed to enhance the immune response. A suitable unit dose for vaccination is 0.5-5 microgram/kg of antigen, and such dose is preferably administered 1-3 times and with an interval of 1-3 weeks. With the indicated dose range, no adverse toxicological effects will be observed with the compounds of the invention which would preclude their administration to suitable individuals.

Each reference disclosed herein is incorporated by reference herein in its entirety. Any patent application to which this application claims priority is also incorporated by reference herein in its entirety. TABLES

Certain pertinent data for preferred polypeptide and polynucleotide embodiments of the invention are summarized in Tables 1 and 2.

Provided in Table 1 are sequence search results providing characterization information regarding certain preferred polynucleotides (denoted as "Assembly") and polypeptides of the invention encoded thereby. For each polynucleotide in Table 1, there is listed the closest homologue of each polypeptide encoded by each ORF in such polynucleotide. This determination of homology is based on a comparison of the sequences of in Table 1 with sequences available in the public domain (see heading entitled "Description" for the homologue name). Where no significant homologue was detected the term "unknown" appears after the heading "Description". Preferred polypeptides encoded by the ORFs of the invention, particularly full length proteins either obtained using such ORFs or encoded entirely by such ORFs, are ones that have a biological function of the homologue listed, among other functions. The analysis used to determine each homologue listed in Table 1 was either BlastP and/or BlastX and/or MPSearch, each of which is well known. Also provided in Table 1 is the amino acid sequence encoded by each ORF. An "Assembly ID" number provides a convenient way to correlate the polynucleotide sequence with the ORF or ORFs it comprises and the polypeptides encoded by these ORFs, as well as to correlate such sequences with other pertinent information provided in Tables 1 and 2. Following the heading "ORF Predictions" the nucleotides at the beginning and end of the ORF sequence are set forth ("Start" and "End" respectively). The direction of translation

33

SUBSTTTUTE SHEET (RULE 26) on the polynucleotide depicted is denoted by an "F" for forward or an "R" for reverse (reverse being translated on the opposite strand from the one depicted). The length of each amino acid sequence is also indicated in a column entitled "Length." Below these data is shown the amino acid sequence encoded by the ORF. If a given polynucleotide comprises one ORF, then in the column entitled "ORF #" there is the numeral one. If it encodes two, there are the numerals one and two in the column, and so on.

TABLE 1

Assembly ID: 3049156 Assembly Length: 495bp

>[SEQ ID NO:l] 3049156 Strep Assembly -- Assembly id#3049156 CTCGGTGATAGAAATAGTGTAATCATGCTTTTCTCTTCTTATCTATACTTTGCTACTTCT ATTATACAAAAAAATAAAGCGCTTGACTAGGGATTTTTAGAAAAAAAGCCTATTTTTTCA AGAAAAATAGGCTTTTTGCGAACGATTGACACAATTGGATTTGGTTAATTCACTCTTAAC GATGGTTTTAAACGATATATATTTTTATATATGTAAATTAAAAACTTCTTTCCTTTCACT TCCTACGACTTTTCAGATACAGATAGCCAAAGAAGTTTTCATAGAGGGCAAAAAAGAGGA GGAAGGCATGAAGAAAGAAGGTCTCTGGCAAAATCATAATAACAGGATCCTTGGCTGGAT CAAAAAGCCAGGTATCATCTCCCACAAAGAGAATTTGATGGAAAAGAGTAAAGAATTGGT CAAAACCAATCAAAACTCCCCCAAGTCCATCATCACAGGTAAGACTACTAGAGCCAGGAG ACTTTTTCGATAAAG

ORF Predictions:

ORF # Start End Direction Length

1 236 385 R 50 aa

>[SEQ ID NO: 88] 3049156-1 ORF translation from 236-385, direction R VGDDTWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK*

Description: unknown

Assembly ID: 3049862 Assembly Length: 529bp

>[SEQ ID NO:2] 3049862 Strep Assembly -- Assembly id#3049862 CTAGAGCAAGTATTTTTCAAACTTTTTCCGAATAAATAGATAGAGCCAGAGAATTTAGTA AACCTAGATTTAAAAATGTGCTATAACATAATATATTGAATCTATAATAGTACACCTTGA CTGCTAAAATATTTCTATAAATTAATTTGACTTTCCTGATAGAGTTATTCACATCTTATT TCAACTCACTATAGAAGGAGGAATAGGAGGATTCTCAGACATCCGGGCATCAGCCCAACT

34

SUBSTTTUTE SHEET (RULE 26) AATGATTTGATTGCTAAGAAAATATTCAGCAATCCAGAAATCACTTGTCAATTTATTCGC GATATGCTGGACTTGCCAGCAAAAAATGTTGACCATTTTGGAGGGAAGCGATATTCACGT ATTACTCTCCATGCCTTACTCAGTGCAGGATTTTTATACCAGTATAGACGTCTTGGCGGA GTTGGATAACGGTACTCAAGTAATTATTGAGATTCAAGTCCATCATCAGAATTTTTCATC AATCACTTGTGGACTTACCTGTGCAGTCAGGTTAATCAAATCTTGAAAA

ORF Predictions:

ORF # Start End Direction Length

1 383 526 F 48 aa

>[SEQ ID NO: 89] 3049862-1 ORF translation from 383-526, direction F VQDFYTSIDVLAELDNGTQVIIEIQVHHQNFSSITCGLTCAVRLIKS*

Description: unknown

Assembly ID: 3112810 Assembly Length: 885bp

>[SEQ ID NO:3] 3112810 Strep Assembly -- Assembly id#3112810 CTCATCATCTGTCAAAAAGCGTTTCTTAGCAGTCGTGATATCCATAAAATAATCTAATAT CACGATTTCCTCATCCGCAAAGAAAGGAAGGCTGACCAACTCCAGTGCCACATCCTTGTA AACTACTTCTTGCATATCAAAGTAGGCAAAGTTGAGGTCAGCAGAATCATACCCAATCTG TTTCAACACTTGACTCTTCATCACTTCAAACTGACCCTGATCTGTCCCTGTAAATAGGCG CAGGCTCGGTAAATTCGATAAAGTCAACTTCTGACTTTCTTCAATGGCTAGCATCGTCTC TCCTTTCTTCAGATTTTTCGATTTAATTTAGTCAATATAGCGCAATTTCCCACGGAAATC TTCTAAGCTCTCGTAGCCTTTTTCCACCATGATTGCTTTCAGTTCATTGGTAAAGCGGTC AAAAGCACTGACGCCTTCTTTGTGAAGGGTCGTTCCCACCTGCACCATACTTGCTCCACA GAGGATGTGTTCAAAGGCATCTCGACCAGTCAGAACGCCACCTGTTCCGATAATTTGGAT TTGAGGATTTAAACGTTGATAAAAGGCGTGAACATTGGCTAGAGCAGTCGGTTTGATGTA TTATCCACCAATTCCACCAAAACCATTCTTAGGCCGAATAACGACAGATTCGTCTTCTAT ATAGAGGCCGTTTCCGATAGAGTTAACGCAGTTGACAAACTTGAGCGGATATTTGTTGAA AATAGCTGCCGCTTGATCAAAGTGAACAATATCAAAATAAGGTGGCAATTTAATTCCAAG AGGTTTGGTGAAGTAAGCAAACACTTCTGCCAAAATCCGGTCTGTTGTCTCAAAATCATA GGCAATCTGAGGTTTACCTGGAACATTTGGACAGGAAAGATTTAG

ORF Predictions:

ORF # Start End Direction Length

1 601 804 R 68 aa

>[SEQ ID NO:90] 3112810-2 ORF translation from 601-804, direction R

35

SUBSTTTUTE SHEET (RULE 26) VFAYFTKPLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESWIRPKN GFGGIGG*

Description:

LLCPYRDA NCBI gi : 511014 - Lactococcus lactis . DIHYDROOROTATE DEHYDROGENASE (EC 1.3.3.1) (DIHYDROOROTATE OXIDASE)

Assembly ID: 3112866 Assembly Length: 925bp

>[SEQ ID NO: 4] 3112866 Strep Assembly — Assembly id#3112866 TCTTGGCCAACTGCATGGAGTTCAGCGGTCAATTTCAACGCACCTGAGAAACAGACCCCT GCACCCCTGAAATCTCAGGAGACATGATGGTCTGGATGGAATCAATAATGAGAAAGTCTG GCTGGATACGCTACCACTTCTGCACGAACACTCTGCATATTGGTCTCTGCATAGAGATAA AACTCACTATCAAAATCACCTAAGCGCTCTGCACGTAGTTTAATCTGCTGGGCAGACTCC TCCCCACTGACATAGAGAACTGTCCCCACTTGGGACAACTGGGTTGAGACTTGTAGGAGA AGAGTTGATTTCCCAATCCCAGGATCCCCACCGATGAGGACGAGACTTTCCTGGTACAAC TCCGCCTCCAAGCACACGGTTGAATTCCTCCATCTCCGTCTTGGTTCGATTGACATTGAT GGAAGTCACCTCAGCTAGTTTCATGGGCTTGGTTTTCTCACCTGTCAAGGACACACGCGC ATTCTTGACCTCGGCAACCTCAACCTCTTCCACAAAAGAAGACCAAGACCCACAGTTGGG GCAACGTCCCAGATATTTAGGGGAATTATACCCACAATTTTGACATACAAATGTCGCTTT TTTCTTTGCGATGACAAACCTCTTTCTATATCTCTAACTCACACTCAATCACTTGGCAAA AATCAATCTTCTCATTTGGCACAAACTGGCGCATGAGCATTCGATGAGCAACAACTACCA CAGTCTGATGTTCTCGATACTTAGACATACATTCTAGAAACCGAGACTTCATTTCCGTAG CTGTCTCATATTGAATAGGACTATTAGGAAGCAACTCCCCCTTGTTTTCTAAAAACAGTC TTCTAGCTGTTTCAAAGTTTTCTATTCCTGTTTTATAGACCTGCCATTCATGTAATAAAG GCTCTACTCTTAAAGGAAGACCCGT

ORF Predictions:

ORF # Start End Direction Length

1 220 513 R 98 aa

>[SEQ ID NO: 91] 3112866-2 ORF translation from 220-513, direction R

VEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGWPGKSRPH

RWGSWDWEINSSPTSLNPWPSGDSSLCQWGGVCPAD*

Description: SMS PROTEIN. - ESCHERICHIA COLI.

Assembly ID: 3113664 Assembly Length: 602bp

36

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 5] 3113664 Strep Assembly -- Assembly id#3113664 TTATGTCAGTGGGATTACGCCTAATCTCCCAGAAGCAGAATTATTATCCGGTCAGGAAAT TAAAACCTTGGNAGACATGAAAACTGCAGCGCAGAAATTGCATGATTTAGGAGCGCCAGC AGTCATTATCAAAGGGAGGCAATCGTCTTAGTCAGGACAAGGCTGTGGATGTCTTTTATG ATGGACAGACCTTTACTATCCTAGAAAATCCAGTTATCCAAGGCCAAAATGCTGGTGCAG GTTGTACCTTTGCCTCTAGCATTGCCAGTCACTTGGTTAAAGGTGATAAACTTTTGCCAG CAGTAGAAAGCTCTAAGGCTTTCGTTTATCGTGCTATTGCACAAGCAGATCAGTATGGAG TAAGACAATATGAAGCAAACAAAAACAACTAAAATCGCCCTTGTATCCCTATTAACCGCC CTTTCTGTGGTTCTAGGTTATTTCTTAAAAATCCCAACACCTACAGGNATTCTAACTCTT TTAGATGCTGGTGTCTTCTTTGCGGCCTTTTACTTTGGTAGTCGTGAAGGAGCGGTAGTC GGAGGACTAGCAAGTTTCTTGCTTGACCTCTTATCAGGCTACCCTCAGTGGATGTTTTTT AG

ORF Predictions:

ORF # Start End Direction Length

1 165 392 F 76 aa

>[SEQ ID NO:92] 3113664-1 ORF translation from 165-392, direction F

VDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLVKGDKLLPAVESSKAFVYRAIAQ

ADQYGVRQYEANKNN*

Description: Thi protein - Rhizobium meliloti

Assembly ID: 3113716 Assembly Length: 456bp

>[SEQ ID NO: 6] 3113716 Strep Assembly -- Assembly id#3113716 CTGGATACTAAGAGAAATCAAAAAAGCACTCTAGGATAGAGGCCTAAAGTGCTTAGTTTC AAGGCTTTACAGCCTATCATATTTAATAAAATATTACAACATCTTGTTGTAGAATTCAAC GACAAGTGCTTCGTTGATTTCTGGGTTGATTTCGTCGCGTTCTGGCAAGCGAGTCAATGA ACCTTCCAATTTTTCAGCGTCGAATGATACGAATGCTGGACGTCCAAGAGTAGCTTCTAC TGCTTCAAGGATTGCTGGAACTTTCAATGATTTTTCACGAACTGAGATCACTTGACCTGC AGTTACGCGGTATGATGGGATATCAACGCGTTTCCCGTCAACAAGGATGTGACCGCTGGT TTACAAATTGGACCAAACTTGACGACCAGTAGTCGCGAGACCAAGACGGTAAACAACGTT ATCCAAACGACGTTCCAAAAGAAGCATAAAGTTGAA

ORF Predictions:

ORF # Start End Direction Length

1 94 291 R 66 aa

37

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO:93] 3113716-1 ORF translation from 94-291, direction R

VISVREKSLKVPAILEAVEATLGRPAFVSFDAEKLEGSLTRLPERDEINPEINEALWEF

YNKML*

Description: 30S RIBOSOMAL PROTEIN S4 (BS4). - BACILLUS SUBTILIS.

Assembly ID: 3174176 Assembly Length: 1961bp

>[SEQ ID N0:7] 3174176 Strep Assembly — Assembly id#3174176 CTAATATAGAATAATCACCGCCGTTGTGAAAGAACGATTGGATGATAATCCAATCGTTCA GGGAAATTGGAAGACCTTGGGTTTCCAATTTAGGCATGAGACACCTTTGGTGGCTGCTGC CGTCCCTCACAAGCTAAGGTGATTGTTGAAAAAGAGGAAAAAGGAGAAGAAATGAAACCA GTAATTTCCATCATCATGGGCTCAAAATCCGACTGGGCAACCATGCAAAAAACAGCAGAA GTCCTAGACCGCTTCGGTGTAGCCTACGAAAAGAAAGTTGTTTCCGCACACCGTACACCA GACCTCATGTTCAAACATGCAGAAGAAGCCCGTAGTCGTGGCATCAAGATCATCATCGCA GGTGCTGGTGGCGCAGCGCATTTGCCAGGCATGGTAGCTGCCAAAACAACCCTTCCAGTC ATTGGTGTGCCAGTCAAGTCTCGTGCTCTTAGTGGAGTGGATTCACTCTATTCTATCGTT CAGATGCCGGGTGGGGTGCCTGTTGCGACCATGGCTATCGGTGAACTCTTTTTTAGGATA TAAAACAGGGTTCGGATAAGTTTTTTTGCAAGGTGGATGATGGCTACATTGTAATGTTTT CCTTGTTCTAACTTAGTCTTAAAAGCAGGTGAAAAGTGAGGGCATGCTTTGGCAGCTTGT ATGAGTACCTACCGCAGATAAGGGGAACCCCGTTTGACCATCCTCCCAGCTAAATCAATC TGACCTGACTGATAAATAGAAGAATCCAGTCCAGCGAAAGCTTGTAATTGAGCAGGATTA TCAAAGGCATGAATATTTCGAATCTCGGCTAAAATGACCGCCCCTAAACGATTCTCAATC CCAGTAACCGTCGTGATGACCGAGTTTAACTCAGCCATCAAGTCATTGACACATTTTTCC GCCTTGTCAATGAGCCTCTTGTAATGTTTGATGTTTTCATTACACGAGATAAAACGTCTA TGCGTTATCAAACTCATTACCAATTAAAACAAATGTGGTTAGATCCTTTCGGAAATTGTC AAGCGATTGGAGGAAATGAACTAATCCACAGCGGCTTATTCCAAGTATACCACTTGGGCT TTGGCAGTAGCTAACTGCGCTAAATATAATATAAGGAGGAGTAAAATGAAGACAGTTCAA TTTTTTTGGCATTATTTTAAGGTCTACAAGTTCTCATTTGTAGTTGTCATCCTGATGATT GTTCTGGCGACTTTTGCCCAAGCCCTCTTTCCAGTCTTTTCTGGACAAGCGGTGACGCAG CTAGCCAATTTAGTTCAAGCTTATCAAAATGGGCAATCCAGAACTTGTATGGCAAAGCCT ATCAGGAATTCATGGTCAATCTTGGCCTGCTGGTTTTGGGTTCTATTTATCTCTAGGTGT AATATAAACATGTGTCTCATGACGCGCGTGATTGCAGAATCGACCAACGAGATGCGCAAA GGTCTCTTTGGTAAGCTTGCTCAGTTGACGGTTTCTTTCTTTGACCGTCGACAAGATGGC GATATCCTGTCTCATTTTACCAGTGATTTGGATAATATCCTCCAAGCCTTTAACGAAAGC TTGATTCAGGTCATGAGCAATATTGTTTTATACATTGGTCTGATTCTTGTCATGTTTTCG AGAAATGTGACGCTGGCTCTCATCACCATTGCCAGCACCCCATTGGCTTTCCTTATGCTG ATTTTCATCGTGAAAATGGCACGTAAATACACCAACCTCCAGCAGAAAGAGGTAGGGAAG CTCAACGCCTATATGGATGAGAGCATCTCAGGCCAAAAAGCCGTGATTGTGCTAGGAATT CAAGAGGATATGATGGCAGGATTTCTTGAACAAAATGAGCGCGTGCGCAAGGCAACCTTT AAAGGAAGAATGTTCTCAGGAATTCTTTTCCCTGTCATGAATGGGATGAGCCTGATTAAT

38

SUBSTTTUTE SHEET (RULE 26) ACAGCCATCGTCATCTTTGCTGGTTCGGCTGTACTTTTGAA

ORF Predictions:

ORF # Start End Direction Length

1 139 543 F 135 aa

>[SEQ ID NO: 94] 3174176-1 ORF translation from 139-543, direction F VIVEKEEKGEEMKPVISIIMGSKSD ATMQKTAEVLDRFGVAYEKKWSAHRTPDLMFKH AEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSIVQMPGGV PVATMAIGELFFRI*

Description:

PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (EC 4.1.1.21) (AIR C ARBOXYLASE) (AIRC) . - BACILLUS SUBTILIS.

Assembly ID: 3174186 Assembly Length: 375bp

>[SEQ ID NO: 8] 3174186 Strep Assembly -- Assembly id#3174186 CTATCTCCAAGTNCGNTTGGAATNCCTCCGCNANCCACAACTCATCCAAGCACTTTNCAA CGTGNCCTGGTCCGGTCCTCCAGTGCGTCTNACNGCACCTTCAACCTGCNCATGGGTAGG TCACATGGCTTCGGGTCTACGTCATGATACTAAGGCGCCCTATTCAGACTCGGNTNCCCT AGGGCTCCGTCTCTTCAACTTAACCACGCAACAGAACGTNACCCGCCGGTTCATTCTACA AAAGGCAGNCTCTCACCCATTAACGGGCTCGAACTTGTTGTAGGCACACNGCTTCAGGTN CTATTTCACCCCCCTCCCGGGGAGCANCTCAACTGACCCNCACGGCACCGGTGNANNAAA CGGTCACTTAGGGAG

ORF Predictions:

ORF # Start End Direction Length

1 83 283 F 67 aa

>[SEQ ID NO: 95] 3174186-1 ORF translation from 83-283, direction F

VRXXAPSTCX VGHMASGLRHDTKAPYSDSXXLGLRLFNLTTQQNXTRRFILQKAXSHPL

TGSNLL*

Description: unknown

Assembly ID: 3174374 Assembly Length: 665bp

39

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 9] 3174374 Strep Assembly -- Assembly id#3174374 GGGGGGGGTNNNTTCTGGGGCCGGGTGNNTCCTNGAAAAAATGCTGGACTTAACGGTTAA ATCATTTGAATTGGCCTGTGGATTTTAGCTAGCAATCCAGAGCGAGTTTTCTCCAAGACA GACCTCTATGAAAAGATCTGGAAAGAANACTACGTGGATGACACCAATACCTTGAATGTG CATATCCATGCTCTTCGACAGGAGCTGGCAAAATATAGTAGTGACCAAACGCCCACTATT AAGACAGTTTGGGGGTTGGGATATAAGATAGAGAAACCGAGAGGACAAACATGAAACTAA AAAGTTATATTTTGGTTGGATATATTATTTCAACCCTCTTAACCATTTTGGTTGTTTTTT GGGCTGTTCAAAAAATGCTGATTGCGAAAGGCGAGATTTACTTTTTGCTTGGGATGACCA TCGTTGCCAGCCTTGTCGGTGCTGGGATTAGTCTCTTTCTCCTATTGCCAGTCTTTACGT CGTTGGGCAAACTCAAGGAGCATGCCAAGCGGGTAGCGGCCAAGGATTTCCCTCCAATTT GGANGTTCAAGGTCCCTGTTAAATTTCCCCCATTTAGGGGCAACCTTTTAATGAAANTTT CCNTNATTTGCCGGGTANCTTTGAATCCCTNGGAAAAAACCCAACNAAAAAAAGGGCTTA NNCCC

ORF Predictions:

ORF # Start End Direction Length

1 154 294 F 47 aa

>[SEQ ID NO:96] 3174374-1 ORF translation from 154-294, direction F VDDTNTLNVHIHALRQELAKYSSDQTPTIKTVWGLGYKIEKPRGQT*

Description:

REGULATORY PROTEIN VANR. - ENTEROCOCCUS FAECIUM (STREPTOCOCCUS FAECIUM) .

Assembly ID: 3174972 Assembly Length: 989bp

>[SEQ ID NO: 10] 3174972 Strep Assembly — Assembly id#3174972 CTACGATATCTTTGGTCTTTTGTAAGATATGAGGTCCACCCTTATGCGCCTCAGTTGGCA TTTCATGCGATTCAAGAAGTTGCCCCTCTTGATCAACCAAACCATACTTGATGTTGGTTC CACCGATATCAATTGCAACGTAATATGTCATAAATACCTCCTTTTAGATTAGAGGAAGCG CTCCTTGGTTTCACGAATCAAGGCAGCAGCCGCTTCTACAACTGGACGATCTTCTTCAGT CACTGGTGTCAATGGTGAACGAACAGATCCAATATTCAAGCCTTCATTGATTTTCAAGAC TTCTTTGATGACACCGTACATATTTCCATGAGCAGAAGTGAGTTTACCAATGATTGCGTT GATAGCATACTGCAATTCACGCGCTGTTTCTAGGTCCTTATCCGCAATCAACTGATTGAG TTTCAAGAAGAGTTCTGGCATAGCACCATAAGTACCACCGATACCAGCCCTAGCCCCCAT GAGGCGTCCTCCTAGGAACTGCTCATCAGGACCATTAAAGACGATATGGTCTTCTCCACC AAGGCTGACAAAGGTTTGGATATCTTGAACTGGCATAGAAGAGTTCTTCACACCGATAAC ACGAGGATTTTTCAACATTTCTGTGTAAAGGCTTGGAGTCAAAGCAACCCCTGCCAATTG AGGAATGTTGTAAATCACGTAGTCTGTGTTTGGAGCTGCAGAACTGATATCGTTCCAGTA TTTGGCAACTGAGTTATTCTGGCAAGCGGAAATAAATTGGTGGAATCCGTTGCAATAGCA

40

SUBSTTTUTE SHEET (RULE 26) TCTACTCCCAAGCTTTCAGCATGGCGAGCAAGTTCCATACTATCTTTAGTATTATTGCAA GCAACATGGGCAATAATGGTCAATTTACCTTTGGCTACCGCCATGACTTCTTCCAAAATC AACTTGCGATCTTCAACGCTTTGGTAGATACATTCACCAGAAGAACCATTGACATAAGAC CTTGAACACCTTTATCAATGAAGTATTGA

ORF Predictions :

ORF # Start End Direction Length

1 169 678 R 170 aa

>[SEQ ID NO: 97] 3174972-1 ORF translation from 169-678, direction R VIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQ FLGGRLMGARAGIGGTYGAMPELFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNM YGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPWEAAAALIRETKERFL*

Description:

N-ACETYLNEURAMINATE LYASE SUBUNIT (EC 4.1.3.3) (N-ACETYLNEURAMINIC ACID ALDOLAS E) (N-ACETYLNEURAMINATE PYRUVATE LYASE) (NALASE) . - ESCHERICHIA COLI.

Assembly ID: 3175138 Assembly Length: 145Obp

>[SEQ ID NO: 11] 3175138 Strep Assembly — Assembly id#3175138 CTCCATATTTCTTAGCCTTCTCAATTAGGGTCTTGAAGTCTTCGACACCACCGATACGCT TACCAATATCAGCATAGTTCAAGTGACCAGAGTCATGGCTGTGATATCCTTAACTTTTTC CCAACCTTGAGGGTTGTTCATAATGCTACGATAAGCAATGGCACCATCTTGCCAATCAAC TTTCTTGTCTGCATTGGCATCTTCAGTGATAACAACCTTAGCACTTGGAAGTTCCTTCGT GTATTCTGGGAAAACAATGCCCTTATAAGCTTTTTCCCATTGCCATTCAGAGCTGTGGAT TCCTACATAGTTGGCATTTCCGACTGTTTCTTTATAAGCTGTCAAACGAGTCCAGTCATT CGAACCACCACCATAGCTATTTTGAGAGTTACTCCAAACACCAGCAGCAAGCTTATCTGT AGAAACAAATCCATACATGTAACCCTTAGCCAAATCCTTCATTGGATTGGTTACATCGAT ATGATCATCTCCGCTGACATGCGTATTGTTTGACATGGTTGCCCCATCAAACTTAGCACC AGTTTGATCACTAGAAACAGAGACTAAAGCATTGCCGAGGAAACTAATAGAAGAAAGTAG TTTTCTTTCGTCATCAATCTTTTGACCTGGAGTGACTTGATTGTGGTTGACAATCTTGGT CACATCAAAGTGCAATTGATTGTCCACAACTTGCAAGCGTACTGTCATTTCCGCATTGAT TAAGTGAGCATCATCGCGAAGCTTCATCAAGTACTCTGCTGTTGTCTCATTGATTTTTTT ATAAGTGACTTCAGGGGTGATTCGGTGGTTATTGATAAAGACTTGGTTGAATTGTTGCAC CTGTCCTGGCAAAGTATGTCCATTCAAGGTGTATCCCTTGACACGAAGGAAGGCTTGGTC AATTACTGCCTTAAGTACCTTAAACTGGATCGTATCATAAGTCACCTTGCTATCGTCAAC AACCGGACCTGTTTCTTTCTGGGCAGGGGTATCCTCTGGGTTTTACCCTCTCTGTGGCTA TCCGTTTCAACGCTTGAACAACTGGTCGCTCATCGTCATAAGAGCCCGCCTTGAGAAAAA TCTTCTTCTCATTTCTAAGATGGTCATTGACCGCAGCTGGTAGAGTCACTGTGTCAAAGA

41

SUBSTTTUTE SHEET (RULE 26) AGATTGACATCCTTATTTGCCTGGCATTTACCTGACCGTCTGACTTGAAGACTGATAGAG AGACGGTTTGTTGATCCTGTTTCAGGAGCAGCAACACGACTACCTCTATACCAAGTGCTA GTTGTTGGAGATTTATACTCCCAGAACCAGCCATCCTTGTCATAACCGACAAAAACATTA TTATTGGTATCTTTAAATTTCAAGGAGACACCAAAGCGTGATTTGCCCTTTTCAGAATCT TCTTTGAAGGTTAAATCAACAGTTGCATTTCCATTGGCATCAACGGTCAAGCCCTTCTTT TCAAACAGAG

ORF Predictions:

ORF # Start End Direction Length

1 79 945 R 289 aa

>[SEQ ID NO:98] 3175138-1 ORF translation from 79-945, direction R

VTYDTIQFKVLKAVIDQAFLRVKGYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINE

TTAEYLMKLRDDAHLINAEMTVRLQWDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSI

SFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA

AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELP

SAKWITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAMTLVT*

Description: unknown

Assembly ID: 3175860 Assembly Length: 42Obp

>[SEQ ID NO:12] 3175860 Strep Assembly — Assembly id#3175860

CTGCGAGTTGTGAGGCTCCTATTATGTCTCGTGATTAAAATCTCTATAAGGTGATTTTGG

AGGGAAATTATCGGGCGACAGCGGGTAGAGAAGAGATGAAAGAGGCTATTTTGGAATATC

AAGCAAATCCTGCTGCCTTAAAAGATCTCAAAGAAAAGGCTAAGAATATTTCCAGAGAGT

ATTCTGAAGAGCATCTGTTACAAATCTGGTTGGACTTTTATGAGAAACAAGCCGCTTTAG

GGACAAAGTAAAAAGTGAGGTAATCTATGCGAATTGGTTTATTTACAGATACCTATTTTC

CTCAGGTTTCTGGTGTTGCGACCAATATCCCAACCTTGAAAACCCACCTTGAAAACACGG

ACTTGCCTGCATTTNTATCTCATACAATCCACCGAATTTCGATGTCCCCCTCCCTACAAC

ORF Predictions :

ORF # Start End Direction Length

1 51 251 F 67 aa

>[SEQ ID NO:99] 3175860-1 ORF translation from 51-251, direction F VILEGNYRATAGREEMKEAI EYQANPAALKDLKEKAKNISREYSEEHLLQI LDFYEKQ AALGTK*

42

SUBSTTTUTE SHEET (RULE 26) Description: unknown

Assembly ID: 3175918 Assembly Length: 661bp

>[SEQ ID NO: 13] 3175918 Strep Assembly -- Assembly id#3175918

CTCCCCAAACTTTTATTTGAGAGTGAACGGTATAAGAATATGAAACCGGAGGTTAAGGTG

GTTTACTCAGTTTTAAAAGATCGGTTGGAGTTGTCTTTGAGCAAAGGTTGGATTGATGAG

GATGGGACTATTTATTTGATTTATTCCAATTCAAATTTGATGGCACTTTTAGGCTGTTCA

AAGTCAAAATTACTCTCCATGTGAGTTTGAAGTGACATTTTTAGATGATTACCATAAAAA

ACATAACTACCCACTATTTTACGAATCCTATCTTCAAAACGTTATGGAATTCCTTGAAAG

TCAAGACATAAAGAATGGGGTTGATGCCTTTGTAGATGATCATCAAAATCTCGTTTTTGT

TTTATATGGACAAGGCTATCGAGCCGAGGGAAAAGAGGGAATACTTACAACCCAAGTAAC

TGTAAAAGCTTATGATGAAGACAAGAAACCGATTAACTTCGCAAATTTATTAGATTCCTT

AATCGTGTCAGAATATCAAATGGAACCGAATCTTTGGGAGGTCTCCTATGATTGATCTCT

ATCTAAGTAAAAATAGCCGAAGAAATCAACTTCTTTTAGACTTCTTCCAAAACTATGGCA

TCGAGGTATCTTGTCATTCAGTTTCTGAAATGACAAAGGACAAATTAATTGAGATGATGA

G

ORF Predictions:

ORF # Start End Direction Length

1 212 535 F 108 aa

>[SEQ ID NO:100] 3175918-1 ORF translation from 212-535, direction F

VTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQGYRAEG

KEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNL EVSYD*

Description: unknown

Assembly ID: 3811220 Assembly Length: 1429bp

>[SEQ ID NO: 14] 3811220 Strep Assembly — Assembly id#3811220

CTGCCCCTGTAAGGCTGGACGATTGCCTTTCTTAGTATCCGCAAAGAGGTAAACTGAGAA

TAGAGAGGATTTCTCCTTCAATATCTTTGACAGACAGGTTCATCTTGCCTTCTACGTCTG

AAAAAATCCGCATATTGACCAGTTTTCTCACAGCATAGTCCAAATCTTCCTCTTGGTCCT

CTGGTCCAACACCAACCAGCAATAAAAGTCCCTGATTGATTTTTCCCTGAATCTGGCCTT

CTATACTCACTTGGGCTTTTTTAACCCGTTGGATAATGATTTTCATAATAGCCTTTCTAG

TAAGAGCTAGGACAACTAGCCGTTGGTCCGTTTGACAGAGTAAACTTCTGGCACACTCTT

AATTTTATCGACAACCGTGGTCAGTGTAGAGAGGTTGGCAATACCGAAGGACACATGGAT

43

SUBSTTTUTE SHEET (RULE 26) ATTAGCAAACTTCATATCCTTGGTTGGTTGGGCATTGACCGTTGAAATATTCTTGGTTGT ATTTGAAAGAACTTGCAGTACATCGTTCAACAGTCCTGTACGGTTGAGACCGTAGATATC GATATGGGCCATATACTCCTTATTTGAGCTAGAGTACTGGTCTTCCCATTCCACATCAAG GAGACGTTGCTCGTAGTTTTCTTGGGCACGCAGGTTCATACAGTCCACACGGTGAATAGC CACACCACGACCCTTGGTAATGTAGCCAACAATATCGTCACCAGGCACGGGGTTACAACA CTTAGCAATCCGCACTAGGAGACCAGAAGCACCTTCAATAACCACTCCCCCCTCATGCTT GACCTTGGAGAGTTTCTTTATTTTCAACCTTGACCTCGCCACCTTTGACAAGCTCCTCTG CCTCAGCCTTGGCCTTGGCACGCTCTTCCTCACGGCGTTCTTTTTCAGTCAGACGGTTAA AGACGGTAATCGCACCGATTTCCCCAAAACCAATGGCCGCAAAGAGGGAGTCTTCTGTCT TGTAACTGGTCTTTTGCAGAACTTGATCCATGTGGCGCTTGTCCATAAATTTATTTGCCA CATAGCCATTTTCTTGGAACTGAGCCATCAGCATCTCACGACCCTTGTTGACAGACAATT CCTTATCTTGGTTTTTAAAGAACTGGCGAATCTTATTGCGCGCCTTGCTAGTCTTGACCA TATTGAGCCAGTCACGGCTAGGTCCAAAGGAGTTCGGGTTGGCGATAATTTCAACCTGAT CCCCTGTCTTTAACTTGGTTGTCAGTGGAACCATGCGGCCATTGACCTTGGCACCAGTTG CTTTTTCACCGACCTTGGTATGGATTTCGTAGGCAAAATCAATCGGTCCTGAATCTTTGG GAAGAGAACGGACAGCTCCATCTGGGGTAAAAACGTAAATCTCCTCAGCCAGATAGTTTT CCTTAACAGAGTCCACAAATTCCTTAGCATCATCAGCCTGGTCTTGGAG

ORF Predictions:

ORF # Start End Direction Length

1 316 873 R 186 aa

>[SEQ ID NO: 101] 3811220-2 ORF translation from 316-873, direction R

VRKSVPRPRLRQRSLSKVARSRLKIKKLSKVKHEGGWIEGASGLLVRIAKCCNPVPGDD

IVGYITKGRGVAIHRVDCMNLRAQENYEQRLLDVE EDQYSSSNKEYMAHIDIYGLNRTG

LLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTWDKIKSVPEVYSV

KRTNG*

Description: stringent response-like protein - Streptococcus ecruisimilis

Assembly ID: 3811436 Assembly Length: 1513bp

>[SEQ ID NO: 15] 3811436 Strep Assembly -- Assembly id#3811436 CTCTGCAATGATGTACTCAAACATCTCCGCTTCTAGTTCCTCCTTAGGCAGAGGCAATTT CCCACGTCGCATCCGGTTCATAAAGACCGTATGGTTTTCTAAAATCAAACTATACAAACT CATGTGGGGAATATCCAATCCAATGGCTTTAGCCACATTTTCCTTTACTTGCTCCATGGT CTGACCAGGCAGAGCATAAATCAAATCAATGGAGATGTTGTCAAAACCAGCCAGTTTCAG GCGATCGATATTTTCATAAATATCCTTCTCCAAATGACTGCGCCCAATCTTTTTCAACAT CTTATCATCAAAGGTCTGGACACCTAGCGAAACACGATTGACAGCCGAATTTTTCAAAAC AGCTATCTTATCCGCATCCAAATCGCCTGGATTGGCTTCAATGGTCAACTCTTCCAAGAC

44

SUBSTTTUTE SHEET (RULE 26) AGACAAATCCAAGTTTTTAGTCAAGCCATTCAGTAACACCTCCAGTTGCGGAGCCGACAG GGCTGTCGGTGTTCCACCACCGATATAAAGGGTTGACAACTTTTCAATATCATAAGAACG AAACTCTTCCAGCAGATGCTCTAAATAGCTGTCGACTGGCTGATTTTTGATGAAGACCTT TGAAAAATCACAATAATAACAAATCTGGGTACAAAATGGGATGTGCACATAGGCTGACGT TGGTTTTTTCTGCATAGTAATTATTATACCACAAAGACTAGATTCCAGATAAAAATCACC ATCCCCAGATACATAGTCCGTCCGGAGATGGTGATGGTTTATTCTTCTGTTATATCAATC ACAATCTCTTCTGAGTCATCAAGAGCTTCGGCTTTTTCTTGCCATTGTTCCTTGAGATTA TTTAATTGATTTTTTGATGCTTCTGTCGCTTGAAAAGCATAGGATTTAGCTTGAGCAAGT ATACTGTCCACAGTGATTTCACCTGACTCAACCTGTTCTTTTGTTTTCAGAACAAAATCT GTAGCCTGCTCCTTAACTTCTGTCAGTTTTTCACAGACTTGCTCCTTGGCATACTCCGGA TCTTCTCTCAAATCATCTAAAAAATCTTGAGCCTGACTGCAAACTTGTTTGCCCTTATCA CTTGTTAAAAACAAGGCAAGAGCTGCACCTGAAACGGTTCCTAAAAGGATTGAGGATAAT TTACCCATAAGGATTCTCCTTTTTTATTTTTTGAAAAATTTACTTGCAAGACGAAGAGCT GACAGACTTGCACCAGTCTTGAGTGTTTTTGAACCAGCTGATGAAGCTTTCTTGCTCAAG ACACGCGCATGGTCATTGAGGTCTGAAACAGATAGAGATAAATCTGCAACAGCACTGAAG AGTGGATCAATCGTAGCCACCTTGACATTGATATCATCTGCCAAGACATTGACCTTAGCC AACAACTCATTGGTGTGATGCAAGGTCACATCCACATCTGAAGTCAAGGTTTTAATCGTC TTTTCTGTTTCATCGATGACACGACCAAGCTTTTGTACAGTAATGATCAGATAGACCAAA AAGACAATCACAG

ORF Predictions :

ORF # Start End Direction Length

1 1164 1511 R 116 aa

>[SEQ ID NO:102] 3811436-3 ORF translation from 1164-1511, direction R

VIVFLVYLIITVQKLGRVIDETEKTIKTLTSDVDVTLHHTNELLAKVNVLADDINVKVAT

IDPLFSAVADLSLSVSDLNDHARVLSKKASSAGSKTLKTGASLSALRLASKFFKK*

Description: unknown

Assembly ID: 3811984 Assembly Length: 505bp

>[SEQ ID NO: 16] 3811984 Strep Assembly -- Assembly id#3811984

CTCTTGTCAGAGAAATTTACAAAACGTTAGGAGAATAAGATGGCATTTATTGAAAAAGGT

CAAGAAATCGATATGGAAGTCATCAAGGCTGAAACCCAATTGTCTGCAGAAGCCTTGAGA

CTCAAGGAAAGCCGTGACAGGGAATTGGCAGATATTATTTCAGGGGAAGATGACCGTATT

CTCTTGGCTGATTGGTCCTTGCTCTTCTGATAATGAAGAGGCGGTCTTGGAATATGCTCG

CCGTTTATCCGCCTTGCAAAAGAAGGTAGCGGATAAGATTTTCATGGTCATGCGCGTGTA

TACTGCTAAGCCTCGTACCAATGGAGACGGCTATAAAGGGTTGGTTCACCAGCCAGATAC

TTCTAAGGCTCCAACCCTGATTAACGGCTTGCAGGCTGTGCGCCAGTTGCACTACCGCGT

45

SUBSTTTUTE SHEET (RULE 26) TGATTACAGAGACTGGTTTGACAACGGCAGATGAGATGCTTTATCCGTCAAATCTGATCT TGGTGGATGACTTTGGTCACCTACC

ORF Predictions:

ORF # Start End Direction Length

1 134 454 F 107 aa

>[SEQ ID NO: 103] 3811984-2 ORF translation from 134-454, direction F VTGN QILFQGKMTVFS LIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKP RTNGDGYKGLVHQPDTSKAPTLINGLQAVRQLHYRVDYRDWFDNGR*

Description:

PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYR-SENSITIVE (EC 4.1.2.15) (PHOSP HO-2-KETO-3-DEOXYHEPTONATE ALDOLASE) (DAHP SYNTHETASE) (3-DEOXY-D-ARABINO-HEP TULOSONATE 7-PHOSPHATE SYNTHASE) . - ESCHERICHIA COLI.

Assembly ID: 3857228 Assembly Length: 1827bp

>[SEQ ID NO:17] 3857228 Strep Assembly -- Assembly id#3857228

CTCTTTTAACCGTTTTAGCGGTGACACCGAGGATTTTTTCAGGACCCAAGACTTGTCGGG

CAACCGAAACTGGGAGTTCGTCATCTCCAATATGCAGACCAGCAGCATCAACCGCAAGAC

AAACATCCAACCGATCATCGATTATCAAGGGGACCTGATAGGCATCTGTTATTTCCTTGA

CTTGTTTTGCCAGTTGATAATATTGATTGGTTGTGAGATTTTTTTCTCGCAATTGGACTA

TGGTAACCCCTGAACGGCAGGCCGTCTCAACTTTTGCAAGAAAGCTTTCCACGGAATCTT

GATAGCGATTGGTTACCAGATATAGTCTAAGCGCTTCTCTATTCATAAACCTCTCCTTTG

ATGGTATCTAGCCAATTTTCATCTCTTCTTAGGAGCGAAAGCTGATTGAGTACTTGGTAA

CGAAATTCTTCCAATCCCATTCCTTGAACAACTATTTTCTCAGCAGCGATATTGAGATAA

GAGACTGCTAAGCAAGAACTTCAAAACCAGTCTTTCCTTGGCTGAGAAAAACAGCTGTTA

AGGCTCCAACCAAGTCTCCTGTCCCTGTTATCCAGTCTAATTCAGTACAGCCATTCTCAA

GTACAGCAACTTGATTCTCCGAAACAATAAGGTCCTTGGGACCTGTGACTAAGAATGACA

TACCACGATAGGTCTGACACCAGTCTTTCAAGACTTGAAGCAAATCCTCCGTTTCTTGAT

CTTTAGCACTCGCATCGACCCCAACGCCGTGATGCTTTAATCCAACAAGACTTCGAATTT

CTGACATGTTTCCTTTAAGGACCGTAGGTCTATAGTCTAAAAGGTCTTTAACTAAGCTCT

TACGAATGGATGAAGTCGTTACGCCAACCGCATCTACTACCATCGGGAGAGAAGATTGGT

TTGCATACAAAGCTGCCATGCGGATTGCTTTTTCCTTCTCAGCTGACAAATGCCCCAAAT

TGATGAAGAGAGCCTGGCTTTGCTTAGTAAAATCAAGAACTTCACGGGGATCATCTGCCA

TGACAGGTTTGCATCCCAGAGCCAAAATCCCATTTGCCAGCATCTCACAAGAAATCTCAT

TGGTCATACAGTGAATGAGGGAACTAGAGCCTATAGGAAAAGGATTTGTCAATGCCTGCA

TCATTCTATCCTTTCAGCAAAGAAATATCCTTGCACTTTTTTAAAGAATTCCTGCTTGAT

TAAAAATCTAAATGCAATAAAGGAAATCGCTGTACCAATCAAGGTTGCTCCGAAAAATCG

46

SUBSmUTE SHEET (RULE 26) AGGCGTGTAGATAAACCAACTAAGCTTAGCAGCCGATCCTGTAAAGAGCACCATAACAGG ATAGGAAACAATAGAACCAATAATACCTGTTCCCACAATTTCTCCCAAGGCAGAAAAGTA AAATTTTCGACCGTACTTATAAAAGAGACCTGCTAGAAGGGCTCCAAAAGTCGCTCCTGT GAGAGATAAAGGAGCTTATCGGAATACCCTTGAGTCGTCATACGGATAAAGGCTGTCACT GTAGCCATAGCCAAGGCATAAACAGGTCCCATCATGATTCCCGCTAGAATATTGACTACA CTGGACATCGGTGCCATTCCCTCAATCCGAAAGATAGGTGTAAGGACTACATCAAGGGCA ATCATCATAGATAAAATGGTCAATTTGTGAACTTGTAGTTGGTGCTTTCTCAAGTTTCTA TTCTTCTCCTTTTTCTAAAGACTGTAAATCGCTCTTCCATGTCTGGTGTTGGTAAGCCAT CTCCCAAAACTTGGCTTCCATATGAACACTGATGTGGAAGGCATCTAGCATTTTTTGCTT ATCTGTCTCATCACTTTCTCGATAGAG

ORF Predictions :

ORF # Start End Direction Length

1 1141 1356 R 72 aa

>[SEQ ID NO:104] 3857228-2 ORF translation from 1141-1356, direction R

VGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEFFKK

VQGYFFAERIE*

Description: unknown

Assembly ID: 3857842 Assembly Length: 485bp

>[SEQ ID NO: 18] 3857842 Strep Assembly -- Assembly id#3857842

CTATTGCCAATCCATATAGCCTATCAGGTGGTCAATAACAACGTGTGGCCATCGCTCGTG

GCCTATCAATGAATCCAGACATCATGCTCTTCGATGAACCAAATTCTGCCCTTGACCCTG

AGATGGTTGGAGAAGTAATTAACGTTATGAAGGAATTGGCTGAGCAAGGCATGACCATGA

TTATCGTAACCCATGAGATGGGATTTGCCCGCCAGGTTGCCAACCGCGTTATCTTTACTG

CAGATGGCGAGTTCCTTGAAGACGGAACACCTGACCAAATCTTTGATAACCCACAACACC

CTCGTCTGAAAGAGTTCTTAGATAAGGTCTTAAACGTCTAAACTCAAACTGCAAGGATTT

CCTTGCAGTTTTTCTACCTCGTATTGGAATTTTTGATTTTTCGGAAAATTATGTTAGAAT

TAAGTTTATGAAATGAGGTTTCCTCATACCTAGCAAGACTAGGAATAAAAATAGAAATTA

GGTAG

ORF Predictions:

ORF # Start End Direction Length

1 45 341 F 99 aa

>[SEQ ID NO: 105] 3857842-1 ORF translation from 45-341, direction F

47

SUBSTTTUTΕ SHEET (RULE 26) VAIARGLSMNPDIMLFDEPNSALDPEMVGEVINVMKELAEQGMTMIIVTHEMGFARQVAN RVIFTADGEFLEDGTPDQIFDNPQHPRLKEFLDKVLNV*

Description:

GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ . - BACILLUS STEAROTHERMOPHILUS .

Assembly ID: 3857996 Assembly Length: 1547bp

>[SEQ ID NO: 19] 3857996 Strep Assembly -- Assembly id#3857996

NTCTTGGGCNCNGGGCGNNTCCTTTGAGGACNACGGTATCGATGACCTTGATCTCAAGTG

CAAGCAGTATCTGAATCTGCAGCAGCACCTGTCCGTGCAAAAGTTCGTCCAACATACAGT

ACAAACGCTTCAAGTTATCCAATTGGAGAATGTACATGGGGAGTAAAAACATTGGCACCT

TGGGCTGGAGACTACTGGGGTAATGGAGCACAGTGGGCTACAAGTGCAGCAGCAGCAGGT

TTCCGTACAGGTTCAACACCTCAAGTTGGAGCAATTGCATGTTGGAATGATGGTGGATAT

GGTCACGTAGCGGTTGTTACAGCTGTTGAATCAACAACACGTATCCAAGTATCAGAATCA

AATTATGCAGGTAATCGTACAATTGGAAATCACCGTGGATGGTTCAATCCAACAACAACT

TCTGAAGGTTTTGTTACATATATTTATGCAGATTAATTTACAGAGGGACTCGAATAGAGC

CCTCTTTTCAGGTTTTACCGTGACAATCCCTATTAAAAATTATATCAAAATCGTGAAAAT

ATTGGAAAAGTATGGTAGAATGAAAATTGTCGTGTGAACGATAATACTCATTCTTGATGA

ATTGTGAAGCAGTTGCCCTTGGGTCGTTTTGCGAGTTGAAGTCAAGAAGAGGAAAAAAAC

AAAAAGGAGAAATACTCATCGAATTTCAATGAAACAACTTCTTGAGGCTGGTGTACACTT

TGGTCACCAAACTCGTCGCTGGAATCCTAAGATGGCTAAGTACATCTTTACTGAACGTAA

CGGAATCCACGTTATCGACTTGCAACAAACTGTAAAATACGCTGACCAAGCATACGACTT

CATGCGTGATGCAGCAGCTAACGATGCAGTTGTATTGTTCGTTGGTACTAAGAAACAAGC

AGCTGATGCAGTTGCTGAAGAAGCAGTACGTTCAGGTCAATACTTCATCAACCACCGTTG

GTTGGGTGGAACTCTTACAAACTGGGGAACAATCCAAAAACGTATCGCTCGTTTGAAAGA

AATTAAACGTATGGAAGAAGATGGAACTTTCGAAGTTCTTCCTAAGAAAGAAGTTGCACT

TCTTAACAAACAACGTGCGCGTCTTGAAAAATTCTTGGGCGGTATCGAAGATATGCCTCG

TATCCCAGATGTGATGTACGTAGTTGACCCACATAAAGAGCAAATCGCTGTTAAAGAAGC

TAAAAAATTGGGAATCCCAGTTGTAGCGATGGTTGACACCAATACTGATCCAGATGATAT

CGATGTAATCATCCCAGCTAACGATGACGCTATCCGTGCTGTTAAATTGATCACAGCTAA

ATTGGCTGACGCTATTATCGAAGGACGTCAAGGTGAGGATGCAGTAGCAGTTGAAGCAGA

ATTTGCAGCTCCAGAAACTCAAGCAGATTCAATTGAAGAAATCGTTGAAGTTGTAGAAGG

TGACAACGCTTAATTTATACAAATAGTAATTACCTAGGAGGGCGGGGCTTAGCCCGGCTC

TCCTATTTTCAAAAAATATAGGAGAATTAAAATGGCAGAAATTACAG

ORF Predictions:

ORF # Start End Direction Length

1 58 456 F 133 aa

48

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 106] 3857996-1 ORF translation from 58-456, direction F VQAVSESAAAPVRAKVRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAA GFRTGSTPQVGAIAC NDGGYGHVAWTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTT TSEGFVTYIYAD*

Description: unknown

Assembly ID: 3858236 Assembly Length: 74Obp

>[SEQ ID NO:20] 3858236 Strep Assembly -- Assembly id#3858236

CTATAAAAAAAAGGGTAACCAGTATGGAGGATGAATGTCTGGAACTATCTGAGAATCTCG

GATTTTGGAAATCAGACCGATCATCATGAGATAAGGAAGGAAAGCACTTGTAAAAAGCAC

TGTAACCACGCCAGTCCCCTGTCCCAAGAGGGTGAGGTGGTAGCGTAAAACCATGCGGAA

AAATCCCTTTTTAGTGGTTGAAATTCTCTCCTTGCTGCGACGTTCTTTTTTGACCTTCTC

CTCACTATTAAGCAGGATCACGTCATAAAAACGAGGAAGGACCTTCTTTTTGGTCAGATA

AAGCAGGAAGAGAGTTAGTCCTATCCAAGCGAGCAGACCCAATATGGCTTCTATTGAAAA

AGGCTCCACTGCTATTTTGTAAAAGATATGAAGAGGATAAAGGAGAAATGGAATGTCTCT

AACTTTGTCAACAATACTTCCAAAAGTCGACTGAAGAAAGAAGATAAATATTAAAGGTAT

GAGAACTCCTATCCCAATCATCACATTCGAAAAAATAGACTGATACTTTCTGAAGACCCT

AGTCTGAGCCAAGAAATGTACTGCCACTACCGTCACTAAAGTAACAGAGACAAATAATAA

GGTCAAGGACAGTAGCATCAAAGGCAAACCCAGCCAAAGAGAAGGAGCTAGACTAATATA

GAGGGCTAGAAAATAAGCTAGGATTGGTACAATTCCAGTTAGAGCTGGCAAGAGGACAGA

CAGTCCTTTAGCAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 1 261 R 87 aa

>[SEQ ID NO: 107] 3858236-1 ORF translation from 1-261, direction R

VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGWTVLFTSAFLPYLMM

IGLISKIRDSQIVPDIHPPYWLPFFL*

Description: unknown

Assembly ID: 3858264 Assembly Length: 2219bp

>[SEQ ID NO:21] 3858264 Strep Assembly -- Assembly id#3858264 ATCGAATTCGTTTTGCAAGTGGCGAAATGCGAACCACGTTTGTGTCTTTATAAGTTTCCA

49

SUBSTTfUTE SHEET (RULE 26) CGTCTTCTTTGTGGACACGACCGTTTGCACCTGAGCCAGAAACGTCGTAGAGGTTTATCC CTAAATCATCCGCTAACTTTCTAGCTGCAGGAGTCGCTCTTAGCTTGTCATCAGCCATGA CCTCTCCAATTCTATTTATGATACAAAGGGCGTCAAAAGCGACTGAAAAATAGGAAATCG ACGATGGCTTCGATGAAGCCAAGGAGATTTATCTTTTTTTCCAAGCTTTTAGCCCGTGCT CTAATCTAAGATATTAAGGACGAAGAGCTCTGCACCTAAAAGATACAAAGTTCTCGTCAG CTTTGTTTTATTTACATAACTTATCTTATGTAACTCTATTCTTTGTTATAAGTTTTTCGG ATTGCATCTTTGATACTTTCAACTGTTGGAATCATTGCACATTTTTAGGTTTTGCGCATA AGGCATCGGCACATCTTCTCCTGCACAACGGCGGATTGGTGCATCTAGATAGTCAAATGC TTCTGATTCTGAAATAATAGCTGAAATTTCACCGATATAGCCACTTGTTTTGTGGGCATC GTTGACCAGAACAACCTTACCAGTCTTCTTCACTGAGTTTATGATGATATCCTTATCAAG CGGAACAAGGGTACGTGGGTCAACAATTTCAACTGAAATTCCTTCTTCAGCTAATTCTTC AGCAGCTTGAACCACACGGCGAAGCATTTTTCCATAAGTGACAACTGTTACATCCGTTCC TTGGCGTTTGATTTCACCAACCCCAAGTGGAATTGTGTAGTCTGGATCAACTGGCACTTC CCCTTTTTGGTTAAATTCTGACTTGTACTCAAGTATAATAACTGGGTTGTTATCACGGAT AGAAGACTTAAGCAGGCCTTTCATGTCCGCAGGTGTTCCAGGTGCCACAACCTTAAGCCC TGGAATGTGAGTAAACCAAGACTCTAGAGATTGTGAGTGCTGGGCGGCAGAGCCAACTCC GTTACCAGCTGCACAACGAACAGTCATTGGAACCTGACCTTTACCACCAAACATGTAACG TGTTTTAGCAGCTTGGTTGACGATATTGTCCATGGCAATAACAGAGAAGTCCATGAAGGT CATATCGACGATTGGACGAAGTCCTGTCATGGCTGCTCCTGCTGCAGCTCCAGAGATGGC AGCTTCAGAAATCGGACAGTCACGGACACGTTCTGGACCAAATTCTTCAAGCATTCCAAC AGAAGTACCGAAGTCTCCTCCGAAGACACCGACGTCTTCTCCCATCAAGAACACATTTTC ATCGCGAACGCATTTCCTCAGACATAGCAAGGATAATGGTGTCACGGAAGGACATTGTTT TTGTTTCCATTTTATCTCTTTCTCCTTAGTCTGCGTAAATATCTTCAAAGGCTGATTCAA GCGGTGGGAATGGGCTTTCCTCTGCAAATTTAACAGAAGCTTCTACTGCTTCCTTTACTT GCGCTTGGATTTCTTCCAATTCTTCGGCACTTGCAATGTTATTTTCAATAAGGTAATTGC GGAGGTTTTCGATTGGATCTTTTTGTTTCCACAATTCCACTTCTTCACGCGTACGATATT TACCAGGGTCAGATGATGAGTGACCGAGCCAGCGATAAGTTACACTTTCAATCAAGACTG GACCATTGCCACTGCGAACATGGTCTATAGCTTTCTGAAATCCTTCATAGACATCGATGA CATTGTTACCGTCTTCGATGAACATTCCAGGAATTCCATAAGCGGCGCTACGTTGATGGA TATGTTCTATATTGGTCATTTTCTTGATATCCGCAGAGATACCGTAACCGTTGTTAATGC AATAGAAAATGACTGGCAGGTTCCAGATAGAAGCCATGTTCACTGCTTCGTGGAAAACAC CTTCATTGGTCGCACCATCTCCAAAGAAGCAGACAACGATTTTACCGGTATTTTGCATTT GCTGACTGAGGGCTGCACCGACAGCGATCCCCATACCACCACCTACGATACCATTGGCAC CAAGGTTCCCAGCATCAAGGTCAGCGATATGCATAGATCCACCTTTCCCTTTACAGGTTC CAGTGTATTTACCAAGGATTTCAGCCATCATTCCGTTGAAGTCAATCCCTTTAGCAATAG CTTGCCCGTGTCCACGGTGGTTTGAGGTAATCAGATCATCTGGATTGAGAGCTACATAG

ORF Predictions:

ORF # Start End Direction Length

1 439 1365 R 309 aa

>[SEQ ID NO:108] 3858264-1 ORF translation from 439-1365, direction R

50

SUBSTTTUTE SHEET (RULE 26) VTPLSLLCLRKCVRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPISEAAISGA AAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCAAGNGVGSA AQHSQSLESWFTHIPGLKWAPGTPADMKGLLKSSIRDNNPVIILEYKSEFNQKGEVPVD PDYTIPLGVGEIKRQGTDVTWTYGKMLRRWQAAEELAEEGISVEIVDPRTLVPLDKDI IINSVKKTGKWLVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDVPMPYAQNL KMCNDSNS*

Description:

2-OXOISOVALERATE DEHYDROGENASE BETA SUBUNIT (EC 1.2.4.4) (BRANCHED- CHAIN ALPHA -KETO ACID DEHYDROGENASE COMPONENT BETA CHAIN (El)) (BCKDH El-BETA) . - BACILL US SUBTILIS.

Assembly ID: 3858610 Assembly Length: 1078bp

>[SEQ ID NO:22] 3858610 Strep Assembly -- Assembly id#3858610

CTAACCCTNGACGGGGCCGCTATCATCAGTCAAACAGCTAAAAATCTTGTCTGCAAAAGT

CTCGATTAACTGAGCTTTTACAAAAGCCGTATTTCCTGGAATAACTTGGAGATTGATCAT

CTTATCCATCAATTCAGCCGATTCGATATTGTCTTCAGCCAGTTGCAGACTTTTTACGAT

TGATTTTGGCAATTCGTAGACATAGGTGTTGTCTCTCAAAGGAATTTTGACAATACCTAA

CTCTTTGATATCTCGGGATACCGTCGCCTGAGTGGCAGTGATACCTGCTTCTTTCAAATG

TTCTACAATTTCTTCTTGCGTGCCGATTTGATAATCTGTCACCAATCTTCTAATTTTTTC

AAGTCTCTCTTTTTTATTCATTTTTAAATTGACTATGCGCCCTCTCTACTGCTTCTTTAA

TCTCAGCAAGAATCTGATTGCTTGCTGACTTTTCTTTTTTCAAATACACTAAAAATTCAA

TATTTCCATGTCCACCTTGGATGGGAGAAAAGTCCAAGCCAAGGACTGAAAAACCTGCCT

CTACTGCCATAGCTGTTACAGATTCAAGGACATTCTGATGAATCTTAGCATCTCGAATAA

TTCCATTTTTCCCAATCTGCTCACGTCCTGCCTCAAACTGAGGTTTGACAAGTGCTACCA

CCTGACCTTGATCAGCCAAGACACGGTGCAAGGCTGGCAAAATCAGACTAAGGGAAATGA

AACTCACATCAATACTGGCAAAGCTCGGCTCCTGCTCGAAATCAGTCTTTTCAGCATAGC

GGAAATTGAACTGCTCCATGCTGACAACTCGTGGGTCTTGGCGTAATTTCCAAGCCAACT

GATTGGTACCAACATCGACTGCAAAGACCAACTTGGCACTATTCTGTAGCATGACATCGG

TAAAACCTCCAGTAGAGGCCCCGATATCAATCGTAGTCGCGCCATCCACCGACAAATCAA

AGACCTGCAAGGCCCTTTTCCAGTTTCAAACCACCACGGCTGACATACTTGAGTTTCTCC

CCCTTGAGTTTTAATTCGGTGTCATCTGGAATTTCTCTCCTGGCTTGTCAAACCGTTC

ORF Predictions:

ORF # Start End Direction Length

1 374 949 R 192 aa

>[SEQ ID NO:109] 3858610-2 ORF translation from 374-949, direction R

VDGATTIDIGASTGGFTDVMLQNSAKLVFAVDVGTNQLA KLRQDPRWSMEQFNFRYAE

KTDFEQEPSFASIDVSFISLSLILPALHRVLADQGQWALVKPQFEAGREQIGKNGIIRD

51

SUBSTTTUTE SHEET (RULE 26) AKIHQNVLESVTAMAVEAGFSVLGLDFSPIQGGHGNIEFLVYLKKEKSASNQILAEIKEA VERAHSQFKNE*

Description: cytotoxin/hemolysin 0RF2 tly - Serpula hyodysenteriae

Assembly ID: 3858716 Assembly Length: 928bp

>[SEQ ID NO:23] 3858716 Strep Assembly — Assembly id#3858716

ACTTTCCTGACCTCTGTTTCCAAATAATCTTCCAAATGGACAGAGATCTACCGTTGTTTG

CATCGATAGCTGAGGTCTTTTTTAGAAAATACCATCACTTTTAGAAAATATAAACACATT

TTTCGGATAAGATTAAGGTTAAAAGCAGCTCGTTTATCCAGGGTCTGATGATGGTCTTCA

CGATAAACCACATCCAATAACCAATGCATACTTTCTGCTGACCAATGACCTCGAACACTA

TGGCAAAAGGTCATCAACATCAAGCTTAAAGTTAAAGATAAAATAGCGAACGTCTTGACT

TGTAATACCATCTCTATCAATAGTATTACGAGTCATTCCAATTCCACGCAATTTATGCCA

TTTGGGATGGTTTTGACACAACCACTTAACATCAGAAGACACCCAGTATTCTCGAACTTC

AATCTATCCTCTTTCTATATTCTAACTGAAAGGACAATTCAATGATTCATTTAATAATGA

TTAGCGCCATTGCTCTAGCCATTGGAATTGGTTACCGCACCAAAATCAATATTGGCCTGC

TGGCTATTGCTTTTTCTTACCTCATCGCAACCACTCTCATGGGATTAAGTCCCAAAGAAC

TTCTTCATTTTTGGCCAACCTCACTCTTTTTTACCATTTTTAGCGTCTCTCTCTTTTATA

ACGTTGCAACAACTAACGGTACTCTTGATGTTTTGGCTCAACACATTCTCTACCGCACAC

GCACCCACCCTAACGCCCTCTACATGATTTTATACCTGATGGCAACCCTTTTGTCTGCTT

TAGGTGCTGGATTTTTCACTACTATGGCCGTTTGCTGTCCTCTAGCGATTACCCTCTGTC

AAAAAGCGGACAAACACCCTTTGATTGGAGTCAAAGCGTCAATGGGAACTTCAGGAAGGG

TAATTTGATAACCAAAGGAATAAAATTT

ORF Predictions:

ORF # Start End Direction Length

1 238 402 R 55 aa

>[SEQ ID NO: 110] 3858716-1 ORF translation from 238-402, direction R VSSDVK LCQNHPKWHKLRGIGMTRNTIDRDGITSQDVRYFIFNFKLDVDDLLP*

Description: unknown

Assembly ID: 3859124 Assembly Length: 847bp

>[SEQ ID NO:24] 3859124 Strep Assembly -- Assembly id#3859124 AAAAACGCACCATATCAAAAACTAAAAAGTTTGATATCATGCGTCATGTCTTAAACTAAT

52

SUBSTTTUTE SHEET (RULE 26) TGACTATACTTTCTATTCAAATGAGCTTTTAACCAATTGATTGAGCCAATCCACTCTTAA AACCAAAGGAGCAATTTCTCGGCTTAGCTGACTCTTCTCGGAATCTGAACCATGTACAAC ATTTTGGATAATCTCATTTTCTCCAGCAGCTTTTGCAAAATCACCTCGAATAGTGCCTGG TAAAGCTTCTTCTGGACGAGTTGCACCCATCATGGTCCGCCAAGTTTCGATTACTTTGGG ACCAGAAATGACACCCACAAGAACTGGACCTGAAGTCATGAATTCACGAATCGGTGGGTA AAAACTCTGACCAACCAAGTCCTGATAGTGCTGGTCAATCAACTCTTCTGAAAACCTGTG AACGAAACTCCAATTTTTCGATTGTAAATCCACGTTGTTCGATGCGCTTTAACACTTCAC CCACTAGCCCTCTTTTTACACCATCTGGTTTGATGATAAAGAATGTTTGTTCCATACCCG TCTCCTTTGTCAGCTTCTTTCTTTTATTTTACCACATCTCGTGGAAAAATGGAGAAAGTT TTCAGAAGAGAGAATGAGAGAACCCTCGGGTTCTCTCATTCTCTCTTATTCTACTGTTTC TTCCACAGTGTCAACGGCAGTATCCACAACTACTTCTGTTGTTTCTTCATTTCCTTCTTC CTCTACTGGAGGATTAAGGTATTCTTCTTCGTTGACAGCATGTGGTTCAAGGTTACGGTA ACGGGCCATACCAGTACCAGCTGGGATGATCTTACCGATGAATAACATTTTCCTTTAAAT TCCAAGG

ORF Predictions:

ORF # Start End Direction Length

1 73 453 R 127 aa

>[SEQ ID NO: 111] 3859124-1 ORF translation from 73-453, direction R VDLQSKN SFVHRFSEELIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRT MMGATRPEEALPGTIRGDFAKAAGENEIIQNWHGSDSEKSQLSREIAPLVLRVD LNQL VKSSFE*

Description: NUCLEOSIDE DIPHOSPHATE KINASE (EC 2.7.4.6) (NDK) (NDP KINASE) (ABNORMAL WING DI SCS PROTEIN) (KILLER-OF-PRUNE PROTEIN) . - DROSOPHILA MELANOGASTER (FRUIT FLY)

Assembly ID: 3859244 Assembly Length: 578bp

>[SEQ ID NO:25] 3859244 Strep Assembly -- Assembly id#3859244

ACAACCTAACTACCGNCTAATTCAGCGCGAACTTCTGCAGTAGCTGCTTCAACAACTTCA

CGACGTGAAAGGATGAAGCGGTTTTCTTTAGCGTTAACTTCTTTGATTTTAGTATCAAAT

TCTTGACCTACAAAACGCTCAGCGTTACGTACGAAACGAGTATCCAACATTGAAGCTGGG

ATAAATCCACGAACACCTTCAAATTCTACTGAAAGTCCACCTTTAACGGCACGCGTTCCT

TTAACAGTAACAACTTCTTCTTCGCGACCAACAAGTTTGTCCCATGCTTTGCGAGCTTCA

AGGCGTTTTTTAGATGACAAGGTATGTAACTGTATCAGTATCTTTACCAACTACTTGACG

AAGTACAAGAACATCCAATACTTCTCCTACTTTAACAAAGTCATTGATATCTGCATCACG

ATCGTTTGTCAATTCGCGAAGAGTCAAGACACCCTTCAACACCAGTTCCCAGAAGAATGC

AACGTTAGCTTGAGTCGCATCAACTGTCAATACTTCAGCACTAACACATCACCAGTCTCA

53

SUBSTTTUTE SHEET (RULE 26) ACTTGACTNACGCTATTGAGCANATCTTCAAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 310 462 R 51 aa

>[SEQ ID NO: 112] 3859244-2 ORF translation from 310-462, direction R VLKGVLTLRELTNDRDADINDFVKVGEVLDVLVLRQWGKDTDTVTYLVI*

Description: unknown

Assembly ID: 3859250 Assembly Length: 888bp

>[SEQ ID NO:26] 3859250 Strep Assembly -- Assembly id#3859250

GTAGTTATAGTAGGGGTCGGATTGAAATGCCACNGCGCTTCTTGGAGTTTCTGATACCGT

TTAAAATAGCGTTGGGCATTCTGGTTGGGAGTCAGAGCCTTATCAAGCGCAATCATGATA

GGTTGGTTGGTATAGTAGTTGTCTAGGATAACCTGGTTCTTGGTCGTTAGGCACCTGGTG

GAGGAAGGTTGTCAGCAATTCTCCTTTTTGACGAAATTCTTCAGCGTTGTCTGTCGCCAG

TAACTATTTTTCCTGTTTTTTGAGTTTGTGTCGGTTTTTCTGAAGTTCATTTTCAACACG

ACGAATCAGTTCACTGGCCTGCTGTTTGACGCGGTCGCGCTCAGCCTTATCCTTATAGTA

GGTGTCCAACAAATCAGAAAGATTTGCAAAAGGCTCTCCCACCTGATTTGCAAAAGGAAC

TGGACTGAAGGAAGTCTCAGTCAAGCATGGCTTGGTTTCCTGATTGAAAAAATTTCGGAA

AGCGGAAAGTTTTTCACTAACCAGTATCCTTTCCAATTCATTTGCCGTATCGCGTCCCAG

ACCTTGAAAGAGGCTTTGAAGATTTTTTGCTGTTAGTTCTTGGGTTTGCAGGATTTCAAA

GAGCTTTTCATCCTTGATAGTAAAAGGATTGAGAGATTCTGTACTTGGCGGAGCGATATA

GGTCGATCCTGGAAGTAAGGTGCGGTAGCTATTTTGTGAAAAGCCGACGTGTTTGATAAC

TTCGAGGATTTTATGACTGCTTTTATCCGACCAGTTAGAATATTACTGTGTTTCCCCATA

ATTTCGATAATCAAGGTAGCCTGGATATGGTCTCCAATCTCGTTTTTATTGGAAACTGTA

ATTTCCACAATACGGTCATTTTCCACTTGCTCAATCGACTCAATCAGG

ORF Predictions:

ORF # Start End Direction Length

1 244 402 R 53 aa

>[SEQ ID NO: 113] 3859250-1 ORF translation from 244-402, direction R VGEPFANLSDLLDTYYKDKAERDRVKQQASELIRRVENELQKNRHKLKKQEK*

Description:

54

SUBSTTTUTE SHEET (RULE 26) STRFBP5A NCBI gi : 496253 - Streptococcus pyogenes . Fibrinogen/Fibronectin binding protein

Assembly ID: 3859588 Assembly Length: 513bp

>[SEQ ID NO:27] 3859588 Strep Assembly -- Assembly id#3859588

ATCGAATTTTGTTCTTTCATAGAGAGCTACCTGAGTTCTATTCAAGCTCAGGTAGTACTT

TCTTATAAACTAGACAAACTAACTGTCATTCTACCATCAGATTACAAGACATCATCGTCA

CTCACCTTGGAATTCAATGTCGTACCCCAATGGGTAATTTTACGGTGGGGTTGAGCTAAA

ATTGGTCTGTTTTCATAGATTGTTTGCCATCTATTCCATAGTAGGCCCGTCTTTTTCTCA

ATCTTAACTCGCAGATTTCTCATATTTTCTTTGATTGGGAGGTTGAGGACAAAACCTGCA

GTCTGGTTGCGACCGTTTCCTTCCCAAGAATGACTACGAACAACTTGGTTTCCATCTTTA

TCTACTGGAACTTCTTCCCAAGTTATGGAGTAGCGGGCAATGTAAGCTCCACTGTGTTGA

ATTATCAATGTTTTATCTTTCACAGGGAGTCTGACTGATTGGTTGAACTGGCTTAGAAAC

TTGTGTCGCCGTTTCAGCATTCGTAGCTATAAA

ORF Predictions:

ORF # Start End Direction Length

1 102 443 R 114 aa

>[SEQ ID NO: 114] 3859588-1 ORF translation from 102-443, direction R

VKDKTLIIQHSGAYIARYSITWEEVPVDKDGNQWRSHSWEGNGRNQTAGFVLNLPIKEN

MRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLNSKVSDDDVL*

Description: PNEUMOLYSIN (THIOL-ACTIVATED CYTOLYSIN) . - STREPTOCOCCUS PNEUMONIAE.

Assembly ID: 3859774 Assembly Length: 214bp

>[SEQ ID NO:28] 3859774 Strep Assembly -- Assembly id#3859774

ATCGAATTCTAACATGTGCTTCTCCTTCTATTGTTCCTATCTTTAAAATCTACTCCTTCA

TGCTCCAAGAGCCAAGCTTTCTTTTCCACTCCTGCAGCATAACCTGTCAGACGCTTGCCT

GCTCCCAACACACGATGACAAGGTACTAGGATAGACCAAGGATTGCGTCCCACTGCTCCA

CCAATTGCTTGAGCAGAAGCCACTTGCAGGTCTT

ORF Predictions:

ORF # Start End Direction Length

1 9 131 R 41 aa

55

SUBSTTTUTESHEET(RULE26) >[SEQ ID NO: 115] 3859774-1 ORF translation from 9-131, direction R VLGAGKRLTGYAAGVEKKAWLLEHEGVDFKDRNNRRRSTC*

Description: GLUTAMATE RACEMASE (EC 5.1.1.3). - ESCHERICHIA COLI .

Assembly ID: 3860140 Assembly Length: 1084bp

>[SEQ ID NO:29] 3860140 Strep Assembly — Assembly id#3860140

CTCCAGCAATGGATCCAAGTATGATGGGCGGGATGATGTAAGCTTTCTATAGAAAACACC

TTATAAAAAACACGAAAGGAGGGAATGACTAACCCTTCTTTTTATAATATTCACTTCTAA

GATTGATGGTGAGCTCTCCTAACTTATATGATAAAATAAGACTAGAGGAAAGGAGAAGAA

CATGATCGATGTACAAGAAATTCTGTGCAAGATGACCCCCAATCAGAAGATTAATTATGA

CCGTGTCATGCAGAAAATGGTACAAGCATGGGAAAAAAATGAGTAGCGGCCAACCATTCT

CGTGCATGTTTGCTGTGCCCCTTGTAGTACCTATACACTAGAATATTTGACCAAGTATGC

AGATGTGACCATCTATTTTGCCAATTCTAATATCCATCCCAAGGCAGAATACCATAAGCG

GGTCTATGTCACCAAGAAATTTGTTAGTGATTTTAATGAGCAGACAGGAAATACGGTTCA

GTACCTAGAAGCTCCCTACGAACCCAATTAATACCGAAAACTAGTTAGGGGGCTAGAGGA

GGAGCCCGAAGGTGGCGACCGTTGCAAGGTTTGTTTTGACTACCGACTGGATAAAACAGC

GCAAGTGGCTATGGACTTGGGCTTTGACTACTTTGGTTCAGCCTTGACCATCAGTCCTCA

TAAGAATTCTCAAACTATCAATAGCATCGGAATCGATGTGCAAAAAATTTACACGCCCCA

CTATCTTCCCAACGATTTCAAGAAAAATCAAGGCTACAAACGTTCAGTAGAGATGCGTGA

GGAGTATGATATCTATCGTCAATGTTATTGTGGCTGCGTCTATGCAGCCCAAGCCCAGAA

TATTGACCTGGTTTAAGTTGAGTAGGACGCCACAGCATGCTTGCTGGATAAGGATGTTGA

GAAAGACTATTCTCATATCACATTTATAGTAGATTGAAACTAGAATAGTACACCTTTACT

TCTCAAACATTGTTAGAAATCGATTCGGCTGTCCTTATTTCATTTTAATATACTGGTACG

AAATTAGATATATCAATGATAACTTGCCTCAAGGTAGGTTTTTTGATAGTAGAAAAGCGA

TAGA

ORF Predictions:

ORF # Start End Direction Length

1 302 511 F 70 aa

2 605 856 F 84 aa

>[SEQ ID NO:116] 3860140-1 ORF translation from 302-511, direction F

VHVCCAPCSTYTLEYLTKYADVTIYFANSNIHPKAEYHKRVYVTKKFVSDFNEQTGNTVQ

YLEAPYEPN*

Description : unknown

56

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 117] 3860140-2 ORF translation from 605-856, direction F

VAMDLGFDYFGSALTISPHKNSQTINSIGIDVQKIYTPHYLPNDFKKNQGYKRSVEMREE

YDIYRQCYCGCVYAAQAQNIDLV*

Description: unknown

Assembly ID: 3860206 Assembly Length: 1124bp

>[SEQ ID NO: 30] 3860206 Strep Assembly — Assembly id#3860206

ATCGAATTCATTGACTGCCTGAAAAGACTTCAACTCGTCTGCCTGATAACCGAAAGACTT

GGTTACTTTGATACCTGATACGGACTCCTGTACCTTGTTATTGAGTTCAGAAAAAGCAGC

TTGGGATTCGCCAAAGGCCTTATGAGTCTTTCTCCCTAGGCGACTAGTCGTATAGGCCAT

GAAAGGTAGGGGGAGAATGGCAACAAGAGTCATCTGCCATGAGATGCTAAAGAGCATGGT

CAACAAAGTCACCAGAGCCGTGATAGAGGCATCCACCGCAGACATGACACCGCCACCTGC

TAAACGAGTCAAGGAATTGATATCATTGGTTGCGTGTGCCATCAGATCACCCGTCCGATA

GGTTTGATAAAAGGCTGACGACATTTTTGTGAAATGCTTAAACAAGCGAGACCGCATGAT

CTGTCCCAAGCAATAAGAGGTCCCAAGGATATACATACGCCACACATAGCGCAAATAGTA

CATACCAAAGGCTGCAAGTAGCAAGTAAAATAGGCTAAGAAGGAGGTCCTGCTGGGTTAA

TTGCCCCGATGTGATGGCATCAATAACCCGCCCCATAACCATAGGAGGAATGAGATTGAG

GACGGAAACCAAGACCAGGGCCACAATCCCGACTAGATAACGGCGTTTTTCTAACTTGAA

AAACCACCAAAATTTTTGAATAATGGACATAAAATCCCTTTCTGGATTGCAAATAGAAAC

CTGAGGCCAATACTCAATGGAAAATCAAAGAGCAAACTAGGAAACTAGCCGCAGGCTGCT

CAAAGCACTGCTTTGAGGTTGTAGATAGAACTGACGAAGTCAGTAACCTACATACGGCAA

GGCGACGTTGACGCCGTTTGAAGAAATTTCCGAAGAATACAAGACCCCAGGTTTTTCTTA

TTTATAAGTTACCACTGTAACAGCACCCTTGTCATATTCAGCAATAAAGATATTGGCTAC

ATTGTCATGCCCTTGTTTACTGAGGTTATCAAGCAACCACTCCTCGCTACGAACAATCGA

TCCCAAGACATCTACTTGAATCACACCGTCAGTCACAACTGGATACTTAGGATTTTCATC

TCCCATTTGCACAACGATGAGTTGCCCATTTTGCTCTTGCACAG

ORF Predictions:

ORF # Start End Direction Length

1 898 1056 R 53 aa

>[SEQ ID NO: 118] 3860206-2 ORF translation from 898-1056, direction R VTDGVIQVDVLGSIVRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTWTYK*

Description: unknown

Assembly ID: 3860270

57

SUBSTTTUTE SHEET (RULE 26) Assembly Length: 1242bp

>[SEQ ID NO: 31] 3860270 Strep Assembly — Assembly id#3860270

TTACCTTCATTGCAGCCATTATTGGTTCTTGTGTCAGCCAGATTTTAAGTATTCTTTATA

AGACACCTGCTGTGGTCTTTATCTTGGCCATTTTGGCACCGCTGGTTCCAGGTTATCTCT

CCTACCGAACAACTGCCTTTTTTGTGACAGGGGACTATAATAAAGCACTGGCAAGTGCGA

CCTTGGTTGTCATGTTGGCTTTGGTAATCTCTATTGGAATGGCTAGCGGAACAGTGATTC

TCAGACTGTATCATTATATAAAAACACATCGAGTATCGTAGACTTTACAGAAATAAAAGA

ATTTTCTGAAAAATGAGATAAATAAATTAACAACGCTTTCTATATGTGCGAGAATACCGC

ACTTATGAAGAAATTGCGGCTGATTTTGGTATCCACGAAAGCAACTTAATCCGTCGGAGC

CAATGGGTTGAAGTAACTCTTGTTCAAAGTGGTGTTACGATTTCAAAAACTCATCTTAGT

GCTGAGAATACGGTGATTGTGGATGCAACAGAGGTAAAAATCAATCGCCCTAAAAAACAA

TTAGCGAATGATTCTGGTAAAAAGAAATTTCACGCTATGAAGGCTCAGGCGATTGTCACA

AGTCAAGGGAGAATTGTTTCTTTGGATATCGCTGTGAACTATTGTCATGATATGAAGTTG

TTCAAAATGAGTCGCAGAAATATCGGACAAGCTGGAAAAATCTTGGCTGATAGTGGTTAT

CAAGGGCCCATGAAGATATATCCTCAAGCACAAACTCCACGTAAATCCAGCAAACTCAAG

CCGCTAATAGCTGAAGATAAAGCTTATAACCATGCGCTATCCAAGGAGAGAAGCAAGGTT

GAGAACATCTTTGCCAAAGTAAAAACGTTTAAAATGTTTTCAACAACCTATCGAAATCAT

CGTAAACGCTTCGGATTACGAATGAATTTGATTGCTGGCATTATCAATTATGAACTAGGA

TTCTAGTTTTGCAGGAAGTCTATTATTTTCCTTATTGTCTGTAAGTCTACTGACCTTGTT

GTTTATCCCAGTCATGGTTTCTAGTTCGGGCTCAGAGTTTCAAAGTGGATGGCAAGAGCA

TCAATTGATTGCTGAGAAGGTTAGTAAAACACTTGACAAGACATTTGATAAGGATGTCAG

AAAAATTCCGACCAGTCAGTTTTATCAAAAATTTGTAGATGAGATGGGAAGGATTTACTC

AGGAAATTTGATCCTCCCAGGAGCTGATAACTGTGAATGGAG

ORF Predictions:

ORF # Start End Direction Length

1 346 966 F 207 aa

>[SEQ ID NO: 119] 3860270-1 ORF translation from 346-966, direction F VREYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVTISKTHLSAENTVIVDATEVKIN RPKKQLANDSGKKKFHAMKAQAIVTSQGRIVSLDIAVNYCHDMKLFKMSRRNIGQAGKIL ADSGYQGPMKIYPQAQTPRKSSKLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKMFST TYRNHRKRFGLRMNLIAGIINYELGF*

Description: ISL2 protein - Lactobacillus helveticus (Probable transposase)

Assembly ID: 3860438 Assembly Length: 1575bp

>[SEQ ID NO:32] 3860438 Strep Assembly -- Assembly id#3860438

58

SUBSTTTUTE SHEET (RULE 26) GTGATGGGGCCTCAGGGAAATGGTTTTGACTTGTCTGACCTTGATGAGCAGAATCAGGTT CTCCTTGTTGGTGGTGGGATTGGTGTTCCACCCTTGCTTGAGGTGGCCAAGGAATTGCAT GAACGTGGAGTGAAAGTAGTGACAGTCCTCGGTTTTGCTAATAAGGATGCTGTTATTTTG AAAACGGAATTGGCTCAGTATGGTCAGGTCTTTGTAACGACAGATGATGGTTCTTATGGC ATCAAGGGAAATGTTCCGTTGTTATCAATGATTTAGATAGTCAGTTTGATGCTGTTTACT CGTGTGGGGCTCCAGGAATGATGAAGTATATCAATCAAACCTTTGATGATCACCCAAGAG CCTATTTATCTCTGGAATCTCGTATGGCTTGTGGGATGGGAGCTTGCTATGCCTGTGTTC TAAAAGTACCAGAAAGCGAGACGGTCAGCCAACGCGTCTGTGAAGATGGTCCTGTTTTCC GCACAGGAACAGTTGTATTATAAGGAGAAAATTATGACTACAAATCGATTACAAGTGTCT CTACCTGGTTTGGATTTGAAAAATCCGATTATTCCAGCATCAGGCTGTTTTGGCTTTGGA CAAGAGTATGCCAAGTACTATGATTTAGACCTTTTAGGTTCTATTATGATCAAGGCGACA ACCCTTGAACCACGTTTTGGGAATCCAACTCCAAGAGTGGCAGAGACGCCTGCTGGTATG CTCAATGCAATTGGCTTGCAAAATCCTGGTTTAGAGGTTGTTTTGGCTGAAAAGCTACCT TGGCTGGAAAGAGAATATCCAAATCTTCCTATTATTGCCAATGTAGCTGGTTTTTCAAAA CAAGAGTATGCAGCTGTTTCTCATGGGATTTCCAAGGCAACTAATATAAAAGCTATCGAG CTCAATATTTCTTGTCCCAATGTTGACCACTGTAATCATGGACTTTTGATTGGTCAAGAT CCAGATTTGGCTTATGATGTGGTGAAAGCAGCTGTGGAAGCCTCAGAAGTGCCAGTTTAT GTCAAATTAACCCCGAGTGTGACCGATATCGTTACTGTCGCAAAAGCTGCAGAAGATGCG GGAGCAAGTGGCTTGACTATGATCATACTCTGGTGGGATGCGCTTTGACCTCAAAACCAG AAAACCAATCTTGGCCAATGGAACAGGTGGAATGTCAGGTCCAGCAGTTTTCCAGTAGCC CTCAAACTCATCCGCCAAGTAGCCCAAACAACAGACCTGCCTATCATTGGAATGGGGGGA GTGGATTCGGCTGAAGCTGCCCTAGAAATGTATCTGGCTGGGGCATCTGCTATCGGAGTT GGAACAGCTAACTTTACCAATCCTTATGCCTGCCCTGACATCATCGAAAATTTACCAAAA GTCATGGATAAATACGGTATTAGCAGTCTGGAAGAACTCCGTCAGGAAGTAAAAGAGTCT CTGAGGTAAACTGCAATCAATCTGTTCTTGATTTTTTATTAGTTTGTAATATGAATTTAG GAGAATTTTGGTACAATAAAATAAATAAGAACAGAGGAAGAAGGTTAATGAAGAAAGTAA GATTTATTTTTTTAG

ORF Predictions:

ORF # Start End Direction Length

1 1 276 F 92 aa

2 460 1128 F 223 aa

>[SEQ ID NO:120] 3860438-1 ORF translation from 1-276, direction F VMGPQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKWTVLGFANKDAVIL KTELAQYGQVFVTTDDGSYGIKGNVPLLSMI*

Description: unknown

>[SEQ ID NO:121] 3860438-3 ORF translation from 460-1128, direction F VKMVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLG

59

SUBSTTTUTE SHEET (RULE 26) SIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEWLAEKLPWLEREYPNLPIIA NVAGFSKQEYAAVSHGISKATNIKAIELNISCPNVDHCNHGLLIGQDPDLAYDWKAAVE ASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMIILWWDAL*

Description: DIHYDROOROTATE DEHYDROGENASE (EC 1.3.3.1) (DIHYDROOROTATE OXIDASE) (DHODEHASE) . - BACILLUS SUBTILIS.

Assembly ID: 3860544 Assembly Length: 776bp

>[SEQ ID NO:33] 3860544 Strep Assembly -- Assembly id#3860544

CTAAGATATCAGAATAACAACGAAATCGAAGCATTAAAAACAAATATTACTTCTAAGAAT

AGCGAGATTGATAGTCAACAAAGCAATATTAAGGATATGACCGTACCTATAATGATCCAA

CTTCTCAGGCTTATAATATTTATGCTCAATTAATTAGTGAGTTAGGTACTGCTCGTTCAA

ACAACAATAAAAGTATTACAGAGCTTGAGGCTAATCTTGGAGTGGCAACAGGTCAAGATA

AAGCTCATAGTATATTAGCGTCAAATGAAGGTACTCTGCATTATCTGGTACCTTTGAAAC

AAGGAATGTCTATTCAGCAGGGGCAAACGATAGCAGAAGTTTCAGGGAAAGAAAAAGGTT

ACTATGTAGAGGCTTTTGTACTTGCGAGTGATATTTCTCGTGTTTCAAAAGGAGCAAAAG

TTGATGTTGCTATTACTGGTGTGAATAGTCAAAAATATGGAACACTAAAGGGACAAGTCA

GACAGATTGATTCAGGAACAATTTCCCAAGAAACGAAAGAGGGGAATATTAGCCTCTATA

AAGTCATGATAGAATTAGAAACCTTAACTCTAAAACATGGAAGCGAGACGGTCATACTCC

AAAAGGATATGCCAGTTGAAGTGCGGATTGTCTATGATAAAGAAACCTATCTTGATTGGA

TTTTAGAAATGTTAAGTTTCAAGCAATAATTGGTTTTAAACCTTAGGTAACCTATAAAAA

CAAATAAGGTAGAGAAAGGATATTTTATCTAAGTTAGCTCACATTACTGCCATTCC

ORF Predictions:

ORF # Start End Direction Length

1 222 689 F 156 aa

>[SEQ ID NO: 122] 3860544-1 ORF translation from 222-689, direction F VATGQDKAHSILASNEGTLHYLVPLKQGMSIQQGQTIAEVSGKEKGYYVEAFVLASDISR VSKGAKVDVAITGVNSQKYGTLKGQVRQIDSGTISQETKEGNISLYKVMIELETLTLKHG SETVILQKDMPVEVRIVYDKETYLDWILEMLSFKQ*

Description: unknown

Assembly ID: 3860558 Assembly Length: 1487bp

>[SEQ ID NO: 34] 3860558 Strep Assembly -- Assembly id#3860558

60

SUBSTTTUTE SHEET (RULE 26) CTGGCCTTTCTCCACCAAAATTGTTCCTTGAGGGAAGGAAGTCAGAACACTAGCCGTTGC ATCTTCCTTTTGCTTTTCAATCGTAATTCCAGATAATTTTTCCCATTCTTTTTGGTGACC CCGGGAGGCAGGATTGAATGGCTTGAGGGAAATGACAAACTTGTCCTAGCAAGAATGGTC AAGGCACCTCCGTCTACAATCAAAATCTGATTTGGGCTTAAATTAACAAAGACCTGTTTT ACTAGATTTTCTCCAGAAGCATCGTCTCGTAAACCAGGCCCCAGCAAGATAACTTCTGCC TTCTCCAATTGCTCTTTTAACAATTGCTGGTCTTGAAGAGAAAAGGCCATAGGCTCAGGT AAATGGCTGTGCAGAGCCGGGATATTTTCCCTGTCCGTTCCAACGGTCACCAATCCTGCA CCGCTTTTTACAGCTGCTAAAGCAGCCATGATGATGGCACCTCCATAAGGATAAGTACCA CCAAGCAGCAGCAGACGACCATAATCTCCTTTATGACTTGAACGAGAACGTTCAATAATA ACTTTTTCTAGTAAGGTTTGATTAATCACTTTCATCCTTTTTCCCTCTCACTTTTATTAT ACAACAAAAAGGAGACGCAGACCTCCTTTTGTAATCTTATATCTAAAATTTAATATTCAT TTCTGCCATTTTAGATATAGCTATAGAAAATACACTCTATTAATCGAATGTTTCTCTTAT TTTCTATCCAATGTCCGAAGTGCTGCTTGATAAGTTTGCTCCATCAGCATGGTAATGGTC ATAGGACCGACACCTCCAGGGACTGGCGTGATATGGCTAGCAAGTGGTGCAACTGCCTCA TAATCAACATCTCCACAGAGCTTCCCATTTTCATCTCGGTTCATCCCAACGTCAATGACA ACCGCACCTGGTTTGACAAAGTCAGCAGTCACAAACTTGGCGCGGCCGATTGCGACTACA AGAATATCTGCTTTAGCAGCCACCTTGGCAAGATTATGAGTTCGTGAGTGGGCCAAGGTT ACTGTCGCATTTTTAGCCAAAAGAAGCTGAGCCATAGGTTTTCCAACGATATTTGAACGA CCGATTACGACCGCATTTTTACCTTCCAAGTCAATCCCATATTCATGAAACATTTCCATA ATTCCTGCAGGTGTCGAGGGAATCATGACTGGATGTCCAGACCAAAGACGTCCCATGTTT AGGGGATGGAAACCATCCACATCCTTTTCTGGGTCAATGGCTAATAAAACCGCCTCTTCA TCGATATGTTTTGGTAATGGCAACTGGACCAAAATCCCATGCCAAGCTGGATCCTGATTA TATTTAGCAATCAGGTCTAACAATTCCTCTTGAGTAATGGTCTCTGGAACTCGCACTACT TCGGTACGGGAACCAGCCGCAAGAGCTGACCTCTCCTTGTTGCGAACGTTAAACTTGGCT GGCTGGATTATCCCCAACCAAAATCACTACCAAACCAGGCACTAGAG

ORF Predictions:

ORF # Start End Direction Length

1 717 1376 R 220 aa

>[SEQ ID NO:123] 3860558-2 ORF translation from 717-1376, direction R VRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLN MGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAWIGRSNIVGKPMAQLLLAKNATVT LAHSRTHNLAKVAAKADILWAIGRAKFVTADFVKPGAWIDVGMNRDENGKLCGDVDYE AVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK*

Description:

5 , 10-methylene-tetrahydrofolate dehydrogenase (folD) homolog - Haemophilus infl uenzae (strain Rd KW20)

Assembly ID: 3860568 Assembly Length: 1634bp

61

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 35] 3860568 Strep Assembly — Assembly id#3860568

CGTGCCTTGGCCAATGATCCAAAAATCTTGATTTCAGACGAGTCGCTTCAAATTTCGGCC

CCTGGACCCTTAAGACCAACCCAAGCAGATTTTGGCCCTTGGTTGCAAGATTTGAACCAA

AAATTAGGCTTGACTGTTGTCCTGATTACGCATGAAATGCAGATTGTCAAAGACATTGCC

AACCGTGTTGCAGTTATGCAGGATGGGCATTTGATTGAAGAGAGTAGTGTGCTTGAAATC

TTCTCAGACCCTAAACAACCTTTGACTCAAGACTTTATCTCAACAGCTACAGGTATTGAC

GAAGCCATGGTCAAAATCGAGAAGCAAGAAATCGTGGAACACTTGTCTGAAAACAGTCTC

TTGGTGCAACTCAAGTACGCTGGATCTTCAACAGACGAGCCACTTTTGAATGAATTGTAC

AAGCATTATCAAGTAATGGCTAATATTCTCTATGGGAATATCGAAATCCTCGATGGTACT

CCTGTTGGAGAATTGGTGGTGGTCTTGTCAGGTGAAAAAGCAGCGCTGGCAGGTGCTCAA

GAAGCCATTCGTCAAGCAGGCGTACAGTTAAAAGTATTGAAGGGAGGACAGTAAGATGGA

ATCATTGATTCAAACCTATTTACCAAATGTCTATAAGATGGGTTGGTCTGGTCAGGCAGG

CTGGGGAACAGCTATCTACCTAACCCTCTATATGACAGTTCTTTCCTTCATTATCGGAGG

CTTCTTGGGGCTAGTGGCAGGTCTCTTTCTCGTCTTGACAGCGCCAGGTGGTGTCTTGGA

GAATAAAGTCGTATTCTGGATTTTAGACAAAATTACCTCAATTTTTCGTGCGGTTCCCTT

TATCATCCTCTTGGCAATCTTGTCACCACTTTCTCACTTGATTGAAAAAACAAGTATCGG

GCCAAATGCAAGCCCTTGTCCCACTTTCTTTTGCAGTCTTTGCCTTCTTTGCCCGTCAGG

TGCAGGTTGTCTTGGCTGAAATGGATGGCGGTGTCATTGAGGCGGGCTCAAAGCGAGCGG

AGCGACTTTCTGGGACATCGTGGGTGTTTACCTATCAGAAGGTCTTCCAGATTTGATCCG

TGTGACGACTGTGACCTTGATTTCCCTTGTTGGGGAAACAGCTATGGCCGGTGCGGTTGG

AGCTGGTGGTATCGGTAACGTAGCCATCGCTTATGGATTTAACCGCTACAATCACGATGT

GACCATCTTGGCAACCATCGTTATCATTTTGATTATCTTTGCAATCCAATTCTTAGGAGA

TTTCTTGACTAAGAAATTGAGCCATAAATAAAAAAGAGCCGTGTGGCTCTTTTTAACTGA

TCAGATTTTCTGGGCAAATTTTTTACTCAAGGCTTGTCCAATCAAGGCACCCACTAGGGC

TCCGATGACAATACTTGCGATAAATAGAAGGACAGTTCCAGGGTTTGGAGCGACCATGAT

GCGGTCGATATATTCTTGGGATTTTCCTCTTGCCAGAAGAGTAGCCATATAGGCTTTGGG

CGCAATCCACATAAGCAAGATTGGTCCTGTTGTACTAAAGGCGAAAATAATGAAAGAAAG

GAAGTTCTTTGTTTTGTCCTTGTATTTTCCTAAATGAGCTACTCCATCTGCTAGGAGGCC

ACAGATAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 1040 1291 F 84 aa

>[SEQ ID NO: 124] 3860568-3 ORF translation from 1040-1291, direction F

VGVYLSEGLPDLIRVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATI

VIILIIFAIQFLGDFLTKKLSHK*

Description: unknown

62

SUBSTmiTE SHEET (RULE 26) Assembly ID: 3860582 Assembly Length: 1087bp

>[SEQ ID NO:36] 3860582 Strep Assembly — Assembly id#3860582

GGAATCATGATGATGTCACTGCTAAATGGTTTCTTAGAAAAAATATTTCCTGAGCGCTTA

CAGATTAGTTTGGGCTTGCTGATTTTATCATTGAGCGGTACAGCTCCCTTCTGGTACCAA

GCCTATCCCTTTGTCTTTGGAACACGGCTTCTCTTTGGTTTGGGTCTTGGGATGATCAAT

GCCAAGGCCATTTCTATTATCAGTGAACGCTACCAAGGAAAAAGGCGAATTCAGATGTTA

GGGCTACGCGCTTCTGCAGAGGTCGTTGGAGCTTCTCTCATTACCTTGGCCGTCGGTCAA

GTTGTTGGCCTTTGGTTGGACAGCTATCTTTCTAGCCTATAGTGCTGGATTTTTGGTGCT

GCCCCTTTATCTGCTCTTTGTCCCTTATGGAAAATCAAAGAAAGAAGTCAAGAAAAGAGC

GAAGGAAGCAAGTCGTTTAACTCGAGAAATGAAAGGCTTGATTTTTACCTTAGCTATCGA

AGCGGCAGTTGTAGTTTGTACCAATACAGCTATTACCATCCGTATTCCAAGTTTGATGGT

GGAAAGAGGATTGGGGGATGCCCAGTTATCTAGTTTTGTTCTTAGTATCATGCAGTTGAT

CGGGATTGTGGCTGGGGTGAGTTTTTCTTTCTTGATTTCTATCTTTAAAGAGAAACTGCT

CCTCTGGTCTGGTATTACCTTTGGCTTGGGGCAAATCGTGATTGCCTTGTCTTCATCCTT

GTGGGTGGTAGTAGCAGGAAGTGTTCTGGCTGGATTTGCCTATAGTGTAGTCTTGACGAC

GGTCTTTCAACTTGTCTCTGAACGAATTCCAGCTAAACTCCTCAATCAAGCAACTTCATT

TGCTGTATTAGGCTGTAGTTTCGGAGCCTTTACGACCCCATTCGTTCTAGGTGCAATTGG

CTTACTAACTCACAATGGGATGTTGGTCTTTAGTATCTTAGGAGGTTGGTTGATTGTAAT

CTCTATCTTTGTCATGTACCTACTTCAGAAGAGAGCTCTAGGATTGATTCCTAAGTTTTT

CTTTTGATACTCAATGAAAATCAAAGAGCAAACTATAGTTGATTGAGTTTGGAATAGTAT

GCTGTAG

ORF Predictions:

ORF # Start End Direction Length

1 356 1027 F 224 aa

>[SEQ ID NO: 125] 3860582-1 ORF translation from 356-1027, direction F

VLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVWCTNTAITIRIPSL

MVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGITFGLGQIVIALSS

SLWWVAGSVLAGFAYSWLTTVFQLVSERIPAKLLNQATSFAVLGCSFGAFTTPFVLGA

IGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRALGLIPKFFF*

Description: unknown

Assembly ID: 3860724 Assembly Length: 119lbp

>[SEQ ID NO: 37] 3860724 Strep Assembly -- Assembly id#3860724 GGATTCCAACGATTATGAACTTGACTGGTCCACTGATTCATCCAATGGCTTTAGAAACAC

63

SUBSTTTUTΕ SHEET (RULE 26) AGCTTTCTTGGAATTAGTCGTCCAGACTCCTAGAAAGTACAGCTCAGGTTTTGAAAATAT GGTCGCAAACGTGCCATCGTGGTTGCTGGACCAGAAGGGTTGGATGAAGCTGGCTTGAAC GGAACAACCNAGATTGCACTTNTTGAAAATGGCGAAATCAGCTTGTCAAGCTTTACTCCA GAGGATTTGGGAATGGAAGGCTATGCTATGGAAGATATTCGTGGTGGGAATGCTCAGGAA AATGCAGAAATTTTGCTTAGCGTTCTGAAAAACGAAGCAAGTCCATTCTTGGAAACGACA GTCTTGAATGCTGGTCTTGGTTTCTATGCTAATGGTAAGATTGATAGCATCAAGGAAGGA GTTGCCTTGGCCCGTCAAGTGATTGCTAGAGGCAAGGCCCTTGAAAAACTCAGACTGTTA CAGGAGTACCAAAAATGAGTCAGGAATTTTTAGCACGAATCTTAGAGCAGAAGGCGCGTG AGGTGGAGCAGATGAAGCTGGAGCAAATCCAGCCTCTGCGCCAGACCTATCGCTTGGCAG AATTTTTGAAGAATCATCAGGACCGCTTGCAGGTAATCGCTGAGTCAAGAAAGCTAGCCC TAGTTTGGGAGATATCAATCTCGATGTGGATATTGTGCAACAGGCCCAGACTTATGAAGA AAACGGAGCAGTGATGATTTCGGTGTTGACAGATGAGGTTTTCTTTAAAGGGCATTTGGA TTATCTACGGGAAATTTCCAGTCAGGTAGAGATTCCGACGCTCAACAAAGACTTTATCAT AGATGAAAAGCAAATCATCCGCGCTCGCAATGCAGGTGCGACAGTTATCTTGCTTATTGT GGCAGCCTTGTCCGAAGAACGCCTCAAGGAACTGTATGACTACGCGACAGAGCTTGGTCT GGAAGTCTTAGTGGAGACTCACAATCTAGCTGAACTAGAGGTAGCCCACAGACTTGGTGG CTGAGATTATCGGGGTCAACAACCGCAACTTGACTACCTTTGAAGTCGACTTGCAGACCA GTGTAGATTTAGCCCCTTACTTTGAGGAAGGTCGCTATTACATTTCTGAATCTGCCATTT TCACAGGGCAGGATGCGGAACGACTAGCCCCATACTTTAACGGAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 139 498 F 120 aa

2 686 1024 F 113 aa

>[SEQ ID NO: 126] 3860724-1 ORF translation from 139-498, direction F

WAGPEGLDEAGLNGTTXIALXENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILL

SVLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK*

Description:

ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.18). - LACTOCOCCUS LACTIS (SUB SP. LACTIS) (STREPTOCOCCUS LACTIS) .

>[SEQ ID NO:127] 3860724-2 ORF translation from 686-1024, direction F VDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREISSQVEIPTLNKDFIIDEKQIIRA RNAGATVILLIVAALSEERLKELYDYATELGLEVLVETHNLAELEVAHRLGG*

Description:

INDOLE-3-GLYCEROL PHOSPHATE SYNTHASE (EC 4.1.1.48) (IGPS). - LACTOCOCCUS LACTIS (SUBSP. LACTIS) (STREPTOCOCCUS LACTIS) .

Assembly ID: 3860858 Assembly Length: 858bp

>[SEQ ID NO: 38] 3860858 Strep Assembly — Assembly id#3860858

ATCGAATTTGCCAACCAAGAAAAATATCCCTTGGATGGTTCTTGGCAATGCAAGCAATAT

CATCGTTCGTGATGGTGGGATTCGTGGATTTGTCATCTTGTGTGACAAGCTCAATAACGT

TTCTGTTGATGGCTATACCATTGAAGCAGAAGCTGGGGCTAACTTGATTGAAACAACTCG

CATTGCCCTCCGTCATAGTTTAACTGGCTTTGAGTTTGCTTGTGGTATTCCAGGAAGCGT

TGGCGGTGCTGTCTTTATGAATGCGGGTGCCTATGGTGGCGAGATTGCTCACATCTTGCA

GTCTTGTAAGGTCTTGACCAAGGATGGAGAAATCGAAACCCTGTCTGCTAAAGACTTGGC

TTTTGGTTACCGCCATTCAGCTATTCAGGAGTCTGGTGCAGTTGTCTTGTCAGTTAAATT

TGCCCTAGCTCCAGGAACCCATCAGGTTATCAAGCAGGAAATGGACCGCTTGACGCACCT

ACGTGAACTCAAGCAACCTTTGGAATACCCATCTTGTGGCTCGGTCTTTAAGCGTCCAGT

CGGGCATTTTGCAGGTCAGTTCGAATTTCAGAAGCTGGCTTGAAAGGCTATCGTATCGGT

GGCGTAGAAGTGTCAGAAAAGCATGCAGGATTTATGATCAATGTCGCAGATGGAACGGCC

AAAGACTACGAGGACTTGATCCAATCGGTTATCGAAAAAGTCAAGGAACACTCAGGTATT

ACGCTTGAAAGAGAAGTCCGGATCTTGGGTGAAAGCCTATCGGTAGCGAAGATGTATGCA

GGTGGTTTTACTCCCTGCAAGAGGTAGTGGGGACCTGACAGAGCCCCGATCGGTTAATCT

ATGAAAAAGAAGGAATTT

ORF Predictions:

ORF # Start End Direction Length

1 610 807 F 66 aa

>[SEQ ID NO:128] 3860858-1 ORF translation from 610-807, direction F

VSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGESLSVAKMYAGGF

TPCKR*

Description: unknown

Assembly ID: 3860890 Assembly Length: 98Obp

>[SEQ ID NO: 39] 3860890 Strep Assembly -- Assembly id#3860890

CTGAAAAAACAGGTTTTGACTATGNAGATTGACAGACGACCGTTCGGAGGTGCAGATATT

GATGCAGCAGGACCTCCCTTACCTGATGAAACCCTTAAGGCAAGTAGGGAAGCAGATGCT

ATCCTACTAGTAGCTATCGGTAGTCCTCAGTATGATGGAGTAGCGGTTCGCCCTGAACAA

GGCCTGATGGCTCTCCGTAAGAACTCAATCTTTACGCTAATATTCGTCCTGTAAAAATCT

TTGACAGTCTCAAGTATTTGTCACCACTCAAACCGGAACGAATTTCTGGTGTAGACTTCG

TCGTGGTGCGTGAATTGACTAGGCGAGATTTACTTTGGAGATCATATCCTTGAAGAGCGC

AAAGCGCGTGATATCAACGACTATAGCTATGAGGAAGTGGAGCGGATTATTCGCAAAGCC

TTTGCCATCGAATTGCAAGAAATCGCAGAAAAATCGTTACTAGTATCGATAAGCAAAATG

65

SUBSTTTUTESHEET(RULE26) TTCTAGCGACCTCAAAACTCTGGCGGAAAGTAGCTGAGGAAGTCGCACAGGATTTCTCAG ATGTAACCTTGGAACACCAGCTGGTAGACTCAGCTGCTATGCTTATGATTACCAATCCTG CTAAGTTTGATGTTATTGTAACGGAGAATCTTTTTGGAGATATTTTATCTGATGAATCAA GCGTCTTATCTGGTACACTTGGGGTTATGCCATCAGCCAGTCATTCTGAAAATGGACCAA GTCTCTATGAACCTATTCACGGTTCAGCACCTGATATTGCAGGTCAAGGAATTGCCAATC CTATTTCCATGATTTTATCAGTTGTCATGATGTTGAGAGATAGTTTCGGACGTTATGAGG ATACAGAGCGTATCAAACGTGCTGTTGAGACAAGTCTGGCGGCAGGAATTTTAACGAGAG ATATAGGAGGTCAGGCTTCAACAAAGGAAATGATGGAAGCTATTATTGCAAGGTTATGAA GTTAGACGAAAAAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 397 486 F 30 aa

>[SEQ ID NO:129] 3860890-2 ORF translation from 397-486, direction F VERIIRKAFAIELQEIAEKSLLVSISKMF*

Description: unknown

Assembly ID: 3860952 Assembly Length: 874bp

>[SEQ ID NO:40] 3860952 Strep Assembly — Assembly id#3860952

TCGATCTAGAGAATTGCTCCAGAGCTTCCTGACCGTCCGCTGCCTCAATAGTTTCATAGC

CACAATCCGTCAAATAATCACTGACCCCCTCACGGATCATCTCTTCATCTTCTACAATTA

AAATTTTCATACTTTAACTGCTCTCTATTTTTTATTTTTCTTAGAATAAATACCTACTCT

ATTTTCTATTATAGTCTCTTGCTGGCCTTTTGTATGTAAGCAACTGACCACTAGATAAAA

CGTTGTGAAATTCCTTTCTCATAAATTCCATAACTTTAGTATATTATATTTAAGCACTAA

AGTACAAAGAAAGCAACTGAAAGCAATGATTTTCACCACTGCTTTCAGATTTATTTTGAA

TTGTTAAATAGCTATTCCTATCCACTATTCTTGAATAGAAACACAAGATGCAATCTTTAT

TCCAGACTCATTTTTTAAAAAATCAAATTTATTCACCATCCAGCAAGAGCTCTTTTGGTT

GTTTTCTAAGGAGATTGCTTGAAGCAAGCGCCATAACGAGAACCACTAGAACCAAGGCAA

GGACAAAAATGATGATAAAGTCTGATGTCTGAATGGAAATGTCTAGGCTCGACAAGGTCT

TGCTAAAGCCATCTACTTCTGCACCGCCACCAAGGTTAGAGGCTTGAGCCGCCTTACTAG

CCTGTTTGGCAACACCTGAAGTCACATTGGCAAGGACAGTGTTTCCAATTCGCACGGGCA

GTGTAATTAGCTAGGAAGTAAGCANAAACTAGAGCAGGGATAGCAATCAAGATAGATTCG

GTGATGAATTGACCCAAGATACTTGCCTGCTTGAGACCAATAGAGAGGAGGATTCCCACT

TCCTTGCCGACGGGCATTGATCCAAAGACTGAGC

ORF Predictions:

ORF # Start End Direction Length

66

SUBSTTTUTE SHEET (RULE 26) 1 449 715 R 89 aa

>[SEQ ID NO: 130] 3860952-1 ORF translation from 449-715, direction R

VRIGNTVLANVTSGVAKQASKAAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLA

LVLWLVMALASSNLLRKQPKELLLDGE*

Description: unknown

Assembly ID: 3860962 Assembly Length: 762bp

>[SEQ ID NO: 41] 3860962 Strep Assembly -- Assembly id#3860962

CTTGTAACGGTCATAAAGTTTCTGCAAACTACCATCCTTGCTCCATTTAGTAACCAAGTT

ATCAAGATAGTCGTTGAGCTCTGTATTTGATTTCTTGGTAACAATACCGTAGTCAGATGG

CTTGAAACTATCATCTAGTAGTTCTGTGCGTTTAACTAGTGTAGCCAGATAGAATAGAGC

GGTCAACGGAAAAGGCATCGATACGATGAGCGTGAAGGGAAGTAATCAATTCTGGGTAGG

AACCAAGTTCGACGAATTTAAACTTCAGACCTTTCTTTTTACCCAGTTCAGTAATCAGGC

GTTGGGTGATAGAACCTTGGGCGACTCCGATGGTTTTGCCGTTTAGGTCCTCAATCTTTT

TGATTTTGGCAGATTTATTGACCAAAAATCCAGAAGCGTCTGTGTAGTAGGGACTGGTAA

AGTTGTAGAGTTTTTTGCGTTCGTCCGTGATGGTAAAGGTCGCGATATCCATATCGACCT

GTTCATTGTCTAGAAGGGGGCCGCGGGTTTGTGCTGTAACCGGCACATAGTGAATCTTGA

CCTTGAGTTCATCAGCTACCATTTTGGCCAAGTCGGTTTCGATACCAGAATAAGTACCGG

TCTTGGGATCTTTGTTAACCAAAATTGGGAACGTCTTGTTTGACACCCGACAACCAGTTC

GCCTCTTTTTTGAATGTCTGCGATACTAGTATTAGCCTGGACTGGTTTGGCAGCAACAAG

GCCGAAAAGGCTAATCAATAATGCTGATAAAAAGAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 152 646 R 165 aa

>[SEQ ID NO:131] 3860962-1 ORF translation from 152-646, direction R VSNKTFPILVNKDPKTGTYSGIETDLAKMVADELKVKIHYVPVTAQTRGPLLDNEQVDMD IATFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTS*

Description: cell adhesion factor PEB1 precursor - Campylobacter jejuni

Assembly ID: 3861268 Assembly Length: 1942bp

67

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO:42] 3861268 Strep Assembly -- Assembly id#3861268

CTCGAATTTTTGGTGCTCCAGAAACGGTTCCAGCAGGAAGCGTTGCTTTCAAGGCATCCA

TGGCAGTGAGTTCTGCAAGCAAACGTCCCTTGACCACACTGGTCAAATGCATGACGTAGC

GGAAGAGCTCCACCTCCATATACTTAGTAACTTGGACACTGGCCGTTTCAGAGATGCGGC

CAATATCGTTACGCCCCAAGTCTACCAACATTCGATGTTCTGCTGTTTCCTTCTCATCAG

AGAGGAGGTCAGTCGCCAAGGCCTTGTCTTCTCCATCCGTAGCCCCTCTTGGTCGCGTCC

CTGCAATCGGATTGGTTGTCACGATGCCATTTTTGACAGAAACCAAACTTTCTGGACTAG

CTCCGATGATTTGATAATCCCCAAAATCATACAAATAAAGGTAATTAGATGGATTAGTCA

CGCGGAGATTTCTGTAGAAGTCAAATGGATTTCCAGTTAACTTCTGCGTGAAGAAAACGC

TGGCTGAGTTACACATCGGAACATATCTCCGTTACGAATCAAGTCACGAGCTGTTTCTAC

CATTCCCTCAAACTTATGTGGAGCGATATGCGGTTTGAAGTCAAGTGGTGATAAATCCAA

GTCTTCAAATTCATTTGGAGCAGGAATGCGTAATTCCTCAAGCACTTGGTTCAAGGATTT

TTCCAAGGCCTCTTGACTGCGCTCACTATAAAGTGCATCCTCTATGACATGTTATCTTCT

CCTTCTTGTTGGTCAAAGACCATATAGCTCTCATAGACAAAGAAATGCATGTCGGGCGTC

CCAATTGTATCCTCAGGGATTTGACCAATTTCTTCATAAAGCGAAATCATATCGTAACCA

ACAAAACCAATGGCTCCCCCACCAAAAGGGAGGTCTGAATGGTGCTGGCTCTTATGAATC

ACTTCATAAAGGAAATCCAAGGGATCCCGATCAATCGCTTGACCATTTTGATAGAGAACT

CCATTTTCAAACTTAATCTCAAAAACTGGATTATAGGCTAGGATAGAAAAACGAGCTGTT

TCCTTGTCTCTCGGAATACTCTCTAAAATAACCTTATGTTGCCCCTTTAAGCGCATATAA

GCCAAGATTGGTGATAAGACATCTCCATGAATGATTCGTTCCATTGTCATTTCCCTTTCA

GTTCTAATTCGAGTTCGTGGCGACTGTATGAAAAATCCCCACGCAAAATAACTTGCGTGA

GGACGAAATTCGCGGTGCCACCTCAATTATAGGATTTCTCCTATCTCTCATTCCTGTCTC

AGATATCTCCTGTAACAGGCTGTGCGATAAAGGGCACTCCCTTGAGAATGATGTTTTCTT

CTCTCGTTTCAGATGAACCCAACTTTACAGCTTTCTCTGCTTGTTTTCAGCAACCACAAG

CTCTCTGTGAGAGAAAAGACTGTAATTTTTCCATCTATTATTTTTTAGCTTCTAGTAATC

TGCAATCGCAGCTAGGTCCTTGCCTCCACGACCAGAGACATTGATGAAGAGATGTTCATC

TCGGTACACCTTTATACTCTTCGAAAATCTCTTCAAACCGCGTCAACGTCGCCTTGCCGT

AGGTATGGTTACTGACTTCGTCAGTTCTATCTGCAACCTCAAAACAGTGTTTTGAGCTGA

CTTCGTCAGTCTTATCGACAACCTCAAAACAGTGTTTTGAGCAGCCTGCAGCTAGTTTCC

TAGTTTGCTCTTTGATTTTCATTGAGTATTATTTCATTTTCTCCTGCAATTGAATTCTTG

CTCAGCTTTTTGTCTTCTATTTCTTTAAAATCAAAGTAGCTCTTTTGTTAATAACTCGAT

CAACAAACATCGTGGTACAAGTATCTACTTTGAAATTTATCAACCACTTAACAACTGATA

CTGTATTTCTAGGAAAACGATGACATTCTTCCTAATAAAACTTCTCATATATAGCATAAA

TTTCTACTCTTTTTAATTCGAT

ORF Predictions:

ORF # Start End Direction Length

1 457 645 R 63 aa

> [ SEQ ID NO : 132 ] 3861268 -1 ORF translation from 457 - 645 , direction R VLEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFRCVTQPAFSSR RS*

Description: ANTHRANILATE SYNTHASE COMPONENT I (EC 4.1.3.27). - LACTOCOCCUS LACTIS (SUBSP. L ACTIS) (STREPTOCOCCUS LACTIS) .

Assembly ID: 3861270 Assembly Length: 1048bp

>[SEQ ID NO:43] 3861270 Strep Assembly -- Assembly id#3861270

CTGTTAAGATTGTTTCCGTGCATCCACATAGGATTTACCTTGTCTGTATGGGCCAATTCA

CCCATCAAAACGCCATAGGTCTCATCTGTCAAGATACTAGACATACCGATATTGTACCAA

AGACTGGTATGACGGAAATAAGTCGATGCGTGTAAACTCAACAAAAAGAGACGCAAGTTG

ATTAGAAAAACCGTCATAGCAATAGCTGCCACAGGAGCTTGAACCACAATCAGTGCCAAC

ATGGCAAACTGGGCACTCCCAGCATAAACAAAGAGACTCATCAAGCCCATCTCAACAGGT

GTCACATAGGGCGCACCGATAGTCCCACAGGCCAGGCCGATACTGACATAGCCAAGAGCC

GTTGGCATGGCTGCCTGCGCCCCCTCCTAAAATCCTTTTTCTTTCATCTTTCTCCTCATA

TTGTCTTAATAATACTCAATGAAAATCAAAGAGCAAACTAGGAAATTAGCCGCAGGNTGC

TCAAAACACCGTTTTGAGGTTGCAGATAGAAACTGACGAAGTCAGCTCAAAACACCGTTT

TGAGGTTGCAGATAGAACTGACGAAGTCAGTAACATATATACGGCAAGGCGACGTTGACG

TGGTTTGAAGAGATTTTCGAAGAGTATTAGAAAATGCCGATAAGGGTCTGCATACCAAGG

CTGGTGAGGATGATGGCAATCCAGCAGACGGCTCCGAGAACAATGGATTTTCCACTGGAT

TTGACCATAGCGACCAGATTAGTTTTGAGACCGATGGCACTCATGGCCATGATAATGAGG

AATTTAGAGAGTTGTTTGAGAGGGGTAAAGAAACTACTAGACACACCGAGAGAGGTCAGA

AGGGTGGTTAGGAGCGATGCAAGGATGAAGTAAAGGATAAAAAGTGGGAAGACTTTTTTC

AGTTGTAAGCCTTGCTTATTTTTTTGCTCGCGACTTTGCCAGTAGGAGAGAAAGAGAGTG

ATGGGGATGATAGCTAGGGTGCGCGTGAGTTTGACAATGGTTGCGGATTCGAGGGTATTG

GTCTGGTAGAGACTGTCCCAAGCGCTAG

ORF Predictions:

ORF # Start End Direction Length

1 627 824 R 66 aa

>[SEQ ID NO:133] 3861270-1 ORF translation from 627-824, direction R

VSSSFFTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQT

LIGIF*

Description: unknown

Assembly ID: 3861288 Assembly Length: 1571bp >[SEQ ID NO:44] 3861288 Strep Assembly -- Assembly id#3861288

AGAGCTGGTAATATTCCCAAAGAAACGGCTCAAATCGAATTAGAAAGCCTTCTGCAAAAA

GGAATCCCAGTCGCTCTGGTATCACGATGCTTTAACGGTATTGCCGAGCCTGTTTATGCC

TACCAGGGTGGGGGCGTACAGTTGCAAAAAGCAGGCGTTTTCTTTGTTAAAGAACTCAAC

GCCCAAAAAGCCCGCTTGAAACTCCTCATCGCCCTCAATGCCGGACTAACAGGACAGGCT

TTGAAAGACTATATGGAAGGCTAATACTCTTCGAAAATCTCTGCAAACCACGTCAGCGTC

GCCTTACCGTATGTAGAGCACAAAATCAGGAAATCTTCTCGATTCCCTGATTTTTTCTAT

TTACGTTTTCGTGTTGAGCTACGTTCTGTCAAACCATGAGGTAAGAGAACTTCACGTTCT

TCCAACTCTTCCTTATGCATAATCTTGGTCAACATACGCATACTAATGGCACCAAGGTCA

TAAAGAGGTTGGGCAATCGTTGTCAAGTTTGGACGGGTAAAGCGTGAGATTTGTGAATCA

TCACTAGTAATAATTCGATAATCTTCTGGCACAGAAACACCTTATCAGCCAAACCGTTCA

AGACTCCTGCTGCCAACTCATCACCTGTCACAACTGCTGCAGTTGCATTTGATGAAATCA

AACGCTCTGCTAAGGCGTAACCATCATCATAGCTATATTTAGATTCAAATACCAAACCCT

CACTATAAGCGATTCCTGCTTTTTTCAAGGTTTCCTTGTAGCCAACTAAACGAACCTTAC

CATTGATGTCATCCACTAGCGGACCGCTAACGAAAGCAATACGCTCATTTTCTTTAGCAA

GGTAACTCACTGCATCAATTGTTGCTTGCTTATAGTCAATATTGACACTTGGCAACTGGT

GCTCAACATCGACAGTTCCTGCGAGAACAATCGGAGTACGTGAACGCGAAAATTCTGAGC

GAATTTTATCTGTCAAGTGATAACCCATATAGATAATGCCATCTACCTGCTTTGAAAAGA

GGGTATTGACAACAGAAACTTCTTTCTCGTTATCTTCATCGCTATTAGCTAGGACAATAT

TGTACTTGTACATTTCTGCAATATCATCAATCCCCTTAGCCAAACTCGAAAAATAACCAT

TGGTAATATTTGGAATCACGACACCGACAGTGGTTGTCTTTTTACTTGCAAGACCACGCG

CAACTGCATTTGGACGATAATCCAAACGATCAATTACCTCTAGCACTTTTTTACGGGTAT

TCTCTTTTACATTTTTATTGCCATTGACCACACGGCTGACCGTCGCCATGGGAAACACCT

GCTTCACGAGCGACATCATAAATGGTTACTGTATCATCTGCATTCATTCCTTTTCCTGTC

CTTTCTATCTCCACACATTCTTTTACAAGTAGAAGTGCTGAATTGAAAGCTCTATATCTT

ACTTACAAAAATGAAGATGTGAAAATTTCGTTTTCATATTTCTACTTATTCCATTCTATC

ACTAATTGTAAACACTTTCAAGTGTTTTTTGAAGATTGATTGAAAAAATTTCATAGAAAA

CCTAGGTTTAG

ORF Predictions :

ORF # Start End Direction Length

1 357 572 R 72 aa

>[SEQ ID NO: 134] 3861288-1 ORF translation from 357-572, direction R

VPEDYRIITSDDSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHG

LTERSSTRKRK*

Description: GLUCOSE-RESISTANCE AMYLASE REGULATOR. - BACILLUS SUBTILIS.

Assembly ID: 3861306

70

SUBSTTTUTE SHEET (RULE 26) Assembly Length: 1682bp

>[SEQ ID NO:45] 3861306 Strep Assembly -- Assembly id#3861306

CTGACGTAAAAAAGATTTTCGGAAAAGTATCATCATCTATTTTAGACCATTTTCTTATAA

TAACCATTTTATTTTTATTTGTCAAGGTCTTTGAATTCTTTCTTAAACAAGCCTTGTAAT

CTCTACTTTTGAAGAATTTATTTTTCCTTACTGACAAGATTTGAGACGGTAGGAATCATT

GAAAATAACCTAGCCAACATCAATCACAATCATTTCTCCTTTCTCAATTACACTAAATTA

TAGTGTATTGAATCTATAACAGTGCACCTTGGCTGCTAAAATATTTCTATAAATTAATTT

GACTTTCCTGATAGAGTTGTTCACATCTTATTTCAATTCACTATACTTTCCCTTATACTC

AATGAAAATCAAAGCGCAAACTAGGAAGCTAGCCACAGGCTGCTCAAAGCACTGCTTTGA

GGTTGTAGATAAGACTGACGAAGTCAGTTACATATATCTACGGCAAGGCGAAGCTGACGC

GGTTTGAAGAGATTTTCGAAGAGTATAAAGTTTGTTTCTGTATCTTTCAGAAAAATAAGG

TATACTGTATGTAAACGATTTCAAAGGAGTCCAGTTATGGCAAAAACATTTTTTATTCCA

AATAAACAGAGCATTTTAGGAGAACAAGAGATTTTGAATGCCAAGTCGATCTTGGCTATG

ATGTAGTCTATCTCCGTCAGCCTCTTAATCGTCTCGAGTATATTGAGTGTGCGATAGTGG

GGCAATCACAATTTCTTTTTAAGGTCAGTTATGCTGATGGTCAAAAGGCTTACCGTGTCG

ATCTTCCTGACCTACTAACAAAGACAGACTGGCAGATTATCAAGTCATTTTTAGATGTTT

TGCTTGCTTATACAGGGACTGATATTGAAGGGCTAGATGGTTTTGATTTTGAAGCTTATT

TCCAAGCAAGTATTCAAGCCTATCTAGCAGACCCTGTAGCTCGTTTTACGATTTGCCAAC

GAATTTTTAATCCTATTTTCTTTAGTCGTGAGAACTTGAAAAGCTTTTTAGAGGCAGATG

GCTTGGCTCAGTTTGAAGCGCGTGTGCGTGCGGTTCAAGAGACAGATGCCTACTTTGCGA

GAGTTTCCTTCTATCAGGATGGAGAAGGAAAAGTGCATGGCGTTTACCATCTAGCTCAAG

GAGTCAAGACAGTTTTACCGAGAGAACCGTTTGTTCCTGCAGCCTATATTGAGCGAATTG

GTGGATAAGGAAGTCCAGTGGGAGATTGACTTGGTTCAAATCACAGGAGACGGCTCTAAA

CCAGAAGACTATGAATCCATAGCTCGCTTGGACTATGCAAAATTCTTAGAGGTATTACCC

CCATCTTTTTACCACCAACTAGACGCCAATCAAATAGAAATACAACCCATCCTAGGACAA

GATTTTAAAACATTAGCACAAGAAAAGTAAAGCAGAAGCAGGTCAATCGACTTGCTTTTT

TGACATAGAAAAAATCCTGCCAAGGATGACAGGATTGCTACTCAATGAAAATCAAAGAGC

AAACTAGGAAGCTAGCCGCAGGCTGTACTTGAGTACGGTAAGGCGAAGCTGACGTGGTTT

GAATTTGATTTTCGAAGAGTATGAATTTTAAAGAAAGGCCAAGATACGAAGATAATCTCC

AATCAGTGCCACTTCAGCTTCCAAGAAGAAGAAGATTATAACTCCCGTTCCCCAAGGACA

GA

ORF Predictions :

ORF # Start End Direction Length

1 717 1208 F 164 aa

2 1201 1410 F 70 aa

>[SEQ ID NO:135] 3861306-1 ORF translation from 717-1208, direction F VGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDVLLAYTGTDIEGLDGFDFEA YFQASIQAYLADPVARFTICQRIFNPIFFSRENLKSFLEADGLAQFEARVRAVQETDAYF ARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIERIGG*

71

SUBSmUTE SHEET (RULE 26) Description: unknown

>[SEQ ID NO: 136] 3861306-2 ORF translation from 1201-1410, direction F

VDKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILGQ

DFKTLAQEK*

Description: unknown

Assembly ID: 3861334 Assembly Length: 3041bp

>[SEQ ID NO: 46] 3861334 Strep Assembly -- Assembly id#3861334 ATCGAATTAAAAATGAGGTATTCAGGCTTGTGATTTTCTATGGAAGTTAATAGTGATTGC CTCTAATGCTTACAAGTGATATTAAAAATAGAGGACCTAGTGATGTCAATCATTTCAACT GATTTAACCCCTTTTCAAATAGATGATACATTGAAAGCAGCCTTGCGAGAAGATGTTCAT TCCGAAGATTACAGTACCAATGCCATTTTTGATCATCATGGCCAAGCCAAGGTGTCGCTT TTTGCCAAGGAAGCTGGTGTTTTAGCGGGGCTAACCGTTTTTCAAAGGGTTTTTACCCTA TTTGATGCCGAGGTGACCTTCCAGAATCCTCATCAATTTAAGGATGGGGATCGTTTGACT AGTGGCGATTTGGTTTTAGAAATCATAGGCTCGGTGAGAAGTCTCTTAACATGTGAACGC GTTGCCTTGAATTTTTTACAACATTTATCAGGGATCGCTTCGATGACAGCTGCTTATGTA GAAGCCTTAGGCGATGATTGCATTAAGGTATTTGATACTCGAAAAACTACTCCTAATTTA CGTCTTTTTGAGAAATATGCCGTGAGAGTTGGCGGTGGCTATAATCATCGCTTTAATTTA TCAGATGCTATCCTGCTAAAAGACAATCACATTGCGGCAGTAGGTAGTGTTCAAAGGGCA ATTGCTCAAGCGCGTGCCTATGCTCCTTTTGTGAAAATGGTCGAGGTGGAAGTGGAAAGC CTTGCTGCTGCCGAAGAAGCTGCGGCGGCGGGTGCTGATATTATCATGTTGGATAATATG TCATTGGAACAGATTGAACAGGCCATTACCCTAATTGCAGGACGTTCTCGGATTGAATGT TCTGGAAATATTGATATGACCACTATTAGCCGTTTTCGTGGTTTAGCGATTGATTACGTC TCCAGTGGTAGTTTAACCCATAGTGCTAAGAGTCTTGATTTTTCCATGAAGGGTTTAACC TACCTTGATGTCTAAGTTGTAAAATAAACTAACTTTTTAAAGGATGTCTTTCCTCTAGAA CGAGTTTTATGTCAGATAGTTTAAACGCCTCTTCAAATATAGTAAAATGAACCAAAAATA GTACACAATGTGGTATAATCTTCTTATGGCATATTCAATAGATTTTCGTAAAAAAGTTCT TTCTTATTGTGAGCGAACAGGTAGTATAACAGAAGCATCACACGTTTTCCAAATCTCACG TAATACCATTTATGGCTGGTTAAAGCTAAAAGAGAAAACAGGAGAGCTAAACCACCAAGT AAAAGGAACAAAACCAAGAAAAGTTGATAGAGATAGACTTAAAAACTATCTTACTGACAA TCCAGACGCTTATTTGACTGAAATAGCTTCTGAATTTGGCTGTCATCCAACTACCATCCA CTATGCGCTCAAAGCTATGGGCTACACTCGAAAAAAGGACCACACCTACTATGAACAAGA CCCAGAAAAAGTAGCCTTATTTCTTAAAAATTTTAATAGTTTAAAGCACCTAGCACCTGT TTAGATTGATGAAACAGGATTCGATACTTATTTTTATCGAGAATATGGTCGCTCATTAAA AGGTCAGTTAATAAGAGGTAAAGTATCTGGAAGAAGATATCAGAGGATTTCTTTGGTTGC AGGTCTAACAAATGGTGAGTTAATCGCTCCAATGACTTACGAAGAGACGATGACGAGCGA CTTTTTTGAAGCATGGTTTCAGAAGTTTCTCTTACCAACATTAACCACACCATCGGTTAT TATTATGGATAATGCAAGATTCCATAGAATGGGTAAGTTAGAACTTTTATGCGAGGAGTT TGGGCATAAACTTTTACCTCTTCCTCCCTACTCGCCTGAGTACAATCTTATTGAGAAAAC ATGGGCTCATATCAAAAAGCACCTCAAAAAGGTATTACCAAGTTGCAATACCTTTTATGA GGCTCTTTTGTCCTGCTCTTGTTTCAATTGACTATAGTTCACGGATACAGTTGGGAAAGA AGTTAAATGTAGTTGGATTTCCACTAAAGGTTGATGAGTAAGTTTTTGTATCTGAACCTG ATTGGCCGCAAGCAGCTAAAAGCAAAGCAGATGCAAAAGTCAGACCTGCACCAAGGACAC GCTTCTTTATGTTCATCTTCTTTCTCCTTAATAGTGGGAATTTGTAAAGTTAATTGAATT TCAAGAATGAAGGTTTTATAAACTTTGGTTATAAAAAACAAAGGATTTCTGTCTTTTATA CAGTCCTCCCCTTGTTTTTATACGATTTCAATTTTAAATTTTTCTGCAAAAAATATTTAT AGTAATTCCACACAGAAAGCATCCCATGGAACTAAGATTTGTTTTTCAAAGACTTCTTGA GCTAGGGTGTTTTCAATCAAGACAGATTTGACTTTTCCTTCTACTGTCAAGTCTTGCTCT TCATTGGACAAGTTAGCCACAACTAGGAAGCGACGGTCGCCATCCTTACGTATATAAGCA AAGACCTTATCAGCCGTATCAAGCAATTCAAAGTCAGCTCGAATTAGCCAACTATTCTCC TTGCGAATTTGGACCAGTTTCTGATAGGTATAGAAAATAGAATCTGGATTTGCCAGCGCT TCTTGGACGTTGATCATCTCGTAATTTGGATTAACTGCCAACCAAGGTTGACCTGTTGAG AAACCAGCGTTTTTGCTCTCGTCCCATTGCATAGGGGTACGGGCATTGTCACGTCCAATA ACACGGATACTGTCCATGATTTCTTGCATCGGAACACCTTTTTCAAGAGCCTCACGCGCA TAGTTGAGAGATTCAATATCTTCTACTTGATCCAGTGTTTCAAACGGATAGTTGGTCATC CCAATCTCCTCACCTTGGTAGATATAAGGAGTTCCTCTCATAAGATGAAGCAAGATTGCA AAGGCTTTGGCAGATTTTTCGCGGTATTCTTGGTCATTTCCCCAGATTGAGACAATACGA GGGAGGTCATGGTTGTTCCAGAAGAGGGAATTCCAGCCGTCCTCAACTCCTAACTCTGTC TGCCATTTGTTGAAGATTTCTTTTAACTTAGCGATATTCAG

ORF Predictions:

ORF # Start End Direction Length

1 76 975 F 300 aa

>[SEQ ID NO:137] 3861334-1 ORF translation from 76-975, direction F

VILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEA

GVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLLTCERVALNF

LQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIL

LKDNHIAAVGSVQRAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQI

EQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV*

Description:

PROBABLE NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE (CARBOXYLATING) (EC 2.4.2.19) (QUINOLINATE PHOSPHORIBOSYLTRANSFERASE (DECARBOXYLATING)) (QAPRTASE) (FRAGMENT). - BACILLUS SUBTILIS (BLAST)

Assembly ID: 3864148 Assembly Length: 4694bp

73

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO:47] 3864148 Strep Assembly -- Assembly id#3864148 TTAATTTAAATTCTTAAAATTTTTTCATAATAATCTCCCTATAAAAATAAAGTCGCCCAA TCAGGCGGCTTATTTTTTTGAAAAATGGGCTTGGTGCCTGAGAATAAATAGCTTAGTGAT AGAAGAAAATGGGGAAATATGGTATAATGAAACGATAGATTTTTGAATAGGAATAAGATC ATGTTTGGATTTTTTAAGAAAGATAAAGGCTGTGGAAGTAGAGGTTCCGACACAGGTTCC TGCTCATATCGGCATCATCATGGATGGCAATGGCCGTTGGGCTAAAAAACGTATGCAACC GCGAGTTTTTGGACATAAGGCGGGCATGGAAGCATTGCAAACCGTGACCAAGGCAGCCAA CAAACTGGGCGTCAAGGTTATTACGGTCTATGCTTTTTCTACGGAAAACTGGACCCGTCC AGATCAGGAAGTCAAGTTTATCATGAACTTGCCAGTAGAGTTTTATGATAATTATGTCCC GGAACTACATGCGAATAATGTTAAGATTCAAATGATTGGGGAGACAGACCGCCTGCCTAA GCAAACCTTCGAAGCTTTAACCAAGGCTGAGGAATTGACTAAGAACAACACAGGATTGAT TCTTAATTTTGCTCTTAACTATGGTGGACGTGCTGAGATTACACAGGCGCTTAAGTTGAT TTCCCAGGATGTTTTAGATGCCAAAATCAACCCAGGTGACATCACAGAGGAATTGATTGG TAACTATCTCTTTACCCAGCATTTGCCTAAGGACTTACGAGACCCAGACTTGATTATCCG TACTAGTGGAGAATTGCGTTTGAGCAATTTCCTTCCATGGCAGGGAGCCTATAGTGAGCT TTATTTTACGGACACCTTATGGCCTGATTTTGACGAAGCGGCCTTGCAGGAAGCTATTCT TGCCTATAATCGTCGCCATCGCCGATTTGGAGGAGTTTAGGAGGAAATATGACCCAGGAT TTACAGAAAAGAACCTTGTTATGCAGGGATTGCCCTGACTATTTTCCTACCAATTTTAAT GATTGGGGGCTCTTGCTTCAGATAGCAATCGGAATCATANCCATGCTAGCCATGCATGAA CTTTTGAAGATGAGAGGTCTAGAGACCATGACGATGGAGGCCTCTTGACCCTCTTTGCAC NTTNGTATTGACCATTCCCCTGGAATCGAATTACCTGACTTTTTTGCCAGTTGATGGGAA TGTGGTTGCCTATAGTGTTTTGATTTCAATCATGTTAGGAACGACCGTTTTTAGCAAGTC TTATACGATTGAGGATGCGGTTTTCCCTCTTGCTATGAGCTTCTACGTGGGCTTTGGATT TAATGCTTTACTAGATGCTCGTGTTGCAGGTTTGGACAAGGCTCTCTTAGCCTTGTGTAT CGTCTGGGCGACAGACAGTGGTGCCTATCTTGTTGGGATGAACTATGGGAAACGAAAGTT AGCACCAAGGGTATCGCCTAATAAAACCCTTGAGGGTGCCTTGGGTGGTATTTTAGGAGC AATTTTAGTAACCATTATCTTTATGATAGTTGACAGTACAGTTGCTCTTCCATATGGAAT TTACAAGATGTCAGTCTTTGCTATTTTCTTTAGCATTGCTGGACAATTTGGTGATTTACT AGAAAGTTCGATCAAACGTCATTTTGGTGTTAAGGATTCTGGGAAATTTATCCCTGGACA TGGTGGTGTTTTGGATCGTTTCGATAGTATGTTGCTTGTATTTCCAATCATGCACTTATT TGGACTCTTTTAATCAAAAGACGGAGGAAACGCTATGCTCGGAATTTTAACCTTTATTCT GGTTTTTGGGATTATTGTAGTGGTGCACGAGTTCGGGCACTTCTACTTTGCCAAGAAATC AGGGATTTTAGTACGTGAATTTGCCATCGGTATGGGACCTAAAATCTTTGCTCACATTGG CAAGGATGGAACGGCCTATACCATTCGAATCTTGCCTCTGGGTGGCTATGTCCGCATGGC CGGTTGGGGTGATGATACAACTGAAATCAAGACAGGAACGCCTGTTAGTTTGACACTTGC TGATGATGGTAAGGTTAAACGCATCAATCTCTCAGGTAAAAAATTGGATCAAACAGCCCT CCCTATGCAGGTGACCCAGTTTGATTTTGAAGACAAGCTCTTTATCAAAGGATTGGTTCT GGAAGAAGAAAAAACATTTGCAGTGGATCACGATGCAACGGTTGTGGAAGCAGATGGTAC TGAGGTTCGGATTGCACCTTTAGATGTTCAATATCAAAATGCGACTTTATCTGGGGCAAA CTGATTACCAATTTTGCAGGTCCTATGAACAATTTTATCTTAGGTGTTGTTGTTTTTTGG GTTTTAATCTTTATGCAGGGTGGTGTCAGAGATGTTGATACCAATCAGTTCCATATCATG CCCCAAGGTGCCTTGGCCAAGGTAGGAGTACCAGAAACGGCACAAATTACCAAGATTGGC TCACATGAGGTTAGCAACTGGGAAAGCTTGATCCAAGCTGTGGAAACAGAAACCAAAGAT AAGACGGCACCGACTTTGGATGTGACTATTTCTGAAAAGGGGAGTGACAAACAAGTCACT GTTACACCCGAAGATAGTCAAGGTCGTTACCTTCTAGGTGTTCAACCGGGGGTTAAGTCA GATTTTCTATCCATGTTTGTAGGTGGTTTTACAACTGCTGCTGACTCAGCTCTCCGAATT CTCTCAGCTCTGAAAAATCTGATTTTCCAACCGGATTTGAACAAGTTGGGTGGACCTGTT GCTATCTTTAAGGCAAGTAGTGATGCTGCTAAAAATGGAATTGAGAATATTCTTGTACTT CTTGGCAATGATTTCCATCAATATTGGGATTTTTAATCTTATTCCGATTCCAGCCTTGGA TGGTGGTAAGATTGTGCTCAATATCCTAGAAGCCATCCGCCGCAAACCATTGAAACAAGA AATTGAAACCTATGTCACCTTGGCCGGAGTGGTCATCATGGTTGTCTTGATGATTGCTGT GACTTGGAATGACATTATGCGACTCTTTTTTAGATAATCGAGGAATATTATGAAACAAAG TAAAATGCCTATCCCAACGCTTCGCGAAATGCCAAGCGATGCTCAAGTTATCAGCCATGC TCTTATGTTGCGTGCTGGTTATGTTCGCCAAGTTTCAGCAGGTGTTTATTCTTATCTACC ACTTGCCAACCGTGTGATTGAAAAAGCTAAAAACATCATGCGCCAAGAATTCGAAAAGAT TGGTGCTGTTGAGATGTTGGCTCCAGCCCTTCTTAGTGCAGAATTGTGGCGTGAATCAGG TCGTTACGAAACCTATGGTGAAGACCTTTACAAACTGAAAAACCGTGAAAAATCAGACTT TATCTTAGGTCCAACTCACGAAGAAACCTTTACAGCTATTGTCCGTGATTCTGTTAAATC TTACAAGCAATTGCCACTCAACCTTTATCAAATTCAGCCCAAGTATCGTGATGAAAAACG CCCACGTAATGGACTTCTTCGTACACGTGAGTTTATCATGAAGGATGCTTATAGTTTCCA CGCTAACTATGATAGTTTGGATAGTGTTTATGATGAGTACAAAGCAGCCTATGAGCGTAT TTTCACTCGTAGTGGTTTAGACTTCAAGGCTATTATTGGTGACGGTGGAGCCATGGGTGG TAAGGATAGCCAAGAATTTATGGCCATTACATCTGCTCGTACAGACCTTGACCGCTGGGT TGTCTTGGACAAGTCAGTTGCCTCATTTGACGAAATTCCTGCAGAAGTGCAAGAAGAAAT CAAGGCAGAATTGCTCAAATGGATAGTCTCTGGTGAAGATACCATTGCTTACTCAAGTGA GTCTAGCTATGCAGCTAACTTAGAAATGGCAACAAACGAGTACAAACCAAGCAACCGTGT TGTCGCTGAAGAAGAAGTTACTCGTGTTGAAACGCCAGATGTTAAATCAATTGATGAAGT TGCAGCCTTCCTCAATGTTCCAGAAGAACAAACGATTAAAACCCTCTTCTACATTGCAGA TGGTGAGCTTGTTGCAGCCCTTCTAGTTGGAAATGACCAACTCAACGAAGTCAAGTTGAA AAATCACTTGGGAGCAAATTTCTTTGACGTTGCTAGCGAAGAAGAAGTGGCGAATGTTGT TCAAGCAGGATTTGGTTCACTTGGACCAGTTGGTTTGCCAGAGAATATTAAAATTATTGC AGATCGTAAGGTGCAAGATGTTCGCAATGCAGTTGTCGGTGCTAACGAAGATGGCTACCA CTTGACTGGTGTGAACCCAGGCCGTGATTTTACTGCAGAATATGTGGATATCCGTGAAGT TCGTGAGGGTGAAATTTCCCCAGATGGACAAGGTGTCCTTAACTTTGCGCGTGGTATTGA GATCGGTCATATTTTCAAACTCGGAACTCGCTATTCAGCAAGCATGGGAGCAGATGTCTT GGATGAAAATGGTCGTGCTGTGCCAATCATCATGGGATGTTACGGTATCGGTGTCAGCCG TCTTCTTTCAGCAGTGATGGAGCAACACGCTCGCCTCTTTGTTAACAAAACGCCAAAAGG TGAATACCGTTACGCTTGGGGAATCAATTTCCCTAAAGAATTGGCACCATTTGATGTGCA TTTGATTACTGTTAATGTCAAGGATGAAGAAGCGCAAGCCTTGACAGAAAAACTTGAAGC AAGCTTGATGGGAG

ORF Predictions:

ORF # Start End Direction Length

1 212 940 F 243 aa

75

SUBSTTTUTE SHEET (RULE 26) 2 1202 1753 F 184 aa

3 2750 3037 F 96 aa

>[SEQ ID NO:138] 3864148-1 ORF translation from 212-940, direction F

VEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFGHKAGMEALQTVTKAANKLGVKVITVY

AFSTENWTRPDQEVKFIMNLPVEFYDNYVPELHANNVKIQMIGETDRLPKQTFEALTKAE

ELTKNNTGLILNFALNYGGRAEITQALKLISQDVLDAKINPGDITEELIGNYLFTQHLPK

DLRDPDLIIRTSGELRLSNFLPWQGAYSELYFTDTLWPDFDEAALQEAILAYNRRHRRFG

GV*

Description : unknown

> [ SEQ ID NO : 139 ] 3864148 -2 ORF translation from 1202 - 1753 , direction F

WAYSVLISIMLGTTVFSKSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCI

VWATDSGAYLVGMNYGKRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGI

YKMSVFAIFFSIAGQFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLF

GLF*

Description: CDP-diglyceride synthetase (cdsA) homolog - Haemophilus influenzae (strain Rd K W20)

>[SEQ ID NO: 140] 3864148-10 ORF translation from 2750-3037, direction

FVDLLLSLRQWMLLKMELRIFLYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKP

LKQEIETYVTLAGWIMWLMIAVTWNDIMRLFFR*

Description: unknown

Assembly ID: 3864172 Assembly Length: 1352bp

>[SEQ ID NO: 48] 3864172 Strep Assembly -- Assembly id#3864172

CTCGTAAGTTCGGAAGCTATCTACACAAGAAATTAACCGCTGCCTAAAGGAGAAGCCATG

TCAACATATAACTGGGATGAGAAGCATATCCTTACCTTTCCTGAAGAAAAAGTAGCCCTT

TCTACTAAGGATGTCCATGTTTACTATGGTAAAAATGAATCCATTAAGGGGATTGATATG

CAATTTGAAAGAAATAAAATTACAGCTTTGATTGGTCCGTCGGGATCGGGGAAATCTACC

TACTTACGCAGTCTCAATCGCATGAATGATACCATTGATATTGCTAAAGTAACTGGGCAG

ATTCTCTATCGTGGAATTGATGTCAACCGTCCAGAAATCAACGTTTATGAAATGCGTAAA

CACATTGGAATGGTTTTTCAACGCCCCAATCCATTTGCTAAATCGAATTTACCGTAATAT

TACCTTTGCGCATGAACGTGCTGGAGTTAAGGATAAGCAAGTCCTAGATGAAATCGTAGA

AACCTCCCTTAGTCAGGCTGCCCTTTGGGATCAGGTTAAAGACGATCTCCACAAGTCAGC

76

SUBSTTTUTE SHEET (RULE 26) CTTGACCTTATCAGGTGGTCAGCAACAACGTCTCTGTATCGCTCGTGCCATCTCTGTTAA GCCAGATATCCTCTTAATGGATGAGCCAGCCTCAGCCTTGGATCCGATTGCGACCATGCA ACTAGAAGAGACCATGTTTGAGCTCAAGAAAAACTTTACCATCATCATTGTAACGCATAA TATGCAGCAGGCTGCTCGTGCAAGTGACTATACAGGCTTCTTTTACTTGGGTGATTTGAT TGAGTATGACAAGACTGCAACTATTTTCCAAAATGCCAAGCTACAGTCCACCAATGACTA TGTATCTGGTCACTTTGGTTAGAAAGGAAACCGTATGACAGATGCGATTTTACAGGTATC AGACCTGTCCGTTTATTATAATAAAAAGAAGGCTTTGAATAGTGTTTCCCTATCTTTCCA ACCTAAGGAAATTACAGCCTTGATTGGTCCATCTGGATCAGGGAAGTCAACCCTCCTCAA GTCTCTCAACCGCATGGGAGATCTCAATCCAGAGGTGACCACAACTGGATCCGTGGTGTA CAATGGTCACAACATCTACAGTCCGCGTACAGATACGGTTGAATTACGTAAGGAAATCGG AATGGTTTTCCAACAACCTAATCCTTTCCCTATGACTATCTATGAGAATGTTGTCTACGG GCTTCGTATCAATGGAATTAAGGATAAGCAGGTTCTGGATGAAGCCGTAGAAAAAGCCTT GCAAGGTGCCTCTATCTGGGATGAGGTCAAGGATCGTCTATATGATTCAGCTATTGGATT GTCAGGTGGTCAACAGCAGCGTGTCTGCGTGG

ORF Predictions:

ORF # Start End Direction Length

1 311 862 F 184 aa

>[SEQ ID NO: 141] 3864172-2 ORF translation from 311-862, direction F

VELMSTVQKSTFMKCVNTLEWFFNAPIHLLNRIYRNITFAHERAGVKDKQVLDEIVETSL

SQAALWDQVKDDLHKSALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEE

TMFELKKNFTIIIVTHNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSG

HFG*

Description: HYPOTHETICAL ABC TRANSPORTER (ORF75) . - BACILLUS SUBTILIS. (BLAST)

Assembly ID: 3864180 Assembly Length: 2258bp

>[SEQ ID NO: 49] 3864180 Strep Assembly -- Assembly id#3864180

AACTTCGACCGTGATAAACAAGCTGAGCTTTGACATACTTGTAGCCAACCTAAAAGCCGT

TCTTCAAGGCCTCAAACCAGCTGCAACTCATTCAGGAAGCCTGGATGAAAATGAAGTGGC

TGCCAATGTTGAAACCAGACCAGAACTCATCACAAGAACTGAAGAAATTCCATTTGAAGT

TATCAAGAAAGAAAATCCTAATCCCAGCTGGTCAGGAAATATTATCACAGCAGGAGTCAA

AGGTGAACGAACTCATTACATCTCTGTACTCACTGAAAATGGAAAAACAACAGAAACAGT

CCTTGATAGCCAGGTAACCAAAGAAGTTATAAACCAAGTGGTTGAAGTTGGCGCTCCTGT

AACTCACAAGGGTGATGAAAGTGGTCTTGCACCAACTACTGAGGTAAAACCTAGACTGGA

TATCCAAGAAGAAGAAATTCCATTTACCACAGTGACTCGTGAAAATCCACTCTTACTCAA

AGGAAAAACACAAGTCATTACTAAGGGTGTCAATGGACATCGTAGCAACTTCTACTCTGT

GAGCACTTCTGCCGATGGTAAGGAAGTGAAAACACTTGTAAATAGTGTCGTAGCACAGGA

77

SUBSmUTE SHEET (RULE 26) AGCCGTTACTCAAATAGTCGAAGTCGGAACTATGGTAACACATGTAGGCGATGAAAACGG ACAAGCCGCTATTGCTGAAGAAAAACCAAAACTAGAAATCCTAAGCCAACCAGCTCCTGC TGAGGAAAGCAAAGCTCTTCCTCAAGATCCAGCTCCTGTGGTAATAGAGAAAAAACTTCC TGAAACAGGAACTCACGATTCTGCAGGGACTAGTAGTCGCAGGACTCATGGCCACACTAG CAGCCTATGGACTCACTAAAAGAAAAGAAGACTAAGTCTTTTCGATAAAAAATAAACAGC GAGATTGAAGCTCGCTGTTTATTTTTTAATTAATCACCTAGTCCAAGACGTTCAAAGATA TCATCCACTCGTTTGGTGTAATAAACTGGGTTGAAGATTTCATCGATTTCTTCTTGTGTG AGACGTGATGTTACTTCTGAATCTGCCTCAAGAAGTGGTTTAAAGTCTACTTGGTTGTCC CAAGAGTAGGCTGTTTTTGGTTGCACCAAGTCATAGGCTTGCTCACGGGTCATGCCTTTT TCAATCAATGTCAACATAGCCCGTTGGCTAAAGATAAGACCAAAAGTCGAGTTCATGTTT CGGATCATATTTTCTGGGAAGACTGTCAAGTTCTTGACGATATTTCCAAAACGGTTGAGC ATGTAGTCAATCAAAATGGTCGTATCTGGTGTGATGATACGCTCAGCTGATGAGTGAGAA ATATCGCGTTCGTGCCAGAGAGCGACGTTTTCATAAGCCGTAATCATGTGACCACGAATG ACACGCGCCAGACCAGTCATATTTTCAGAACCGATTGGGTTGCGTTTGTGAGGCATTGCT GAAGACCCTTTTTGCCCTTTAGCAAAGAACTCTTCTACTTCGCGTTGCTCAGATTTTTGT AGACCACGAATCTCAGTCGCCATACGTTCGATTGAAGTCGCAATGCTGGCAAGAACCGCA AAGTACTCAGCGTGAAGGTCACGAGGAAGGACTTGTGTTAAAGATTCCTTGGGCACGGAT GCCAAGATTTATCGCAGACATACTCCTCTACAAATGGTGGGATATTGGCAAAGTTCCCAA CCGCACCAGAAATCTTACCAGCTTCTACACCAGCAGCCGCATGCTCGAAGCGCTCGATAT TGCGTTTCATTTCGCTGTACCAAGTTGCTAATTTAAGACCAAAGGTTGTCGGCTCAGCGT GCACACCATGAGTACGCCCCATCATGATGGTGAACTTGTGCTCCTTGGCCTTGTCAGCGA TGATATTAGTGAAGTTTTCAAGGTCACGACGGATGATGTCGTTGGCCTGCTTGTAGAGGT AACCATAAGCAGTATCCACCACGTCGGTAGAAGTTAACCCATAGTGAACCCACTTGCGCT CTTCACCAAGAGTCTCAGAAACCGCACGCGTGAAAGCCACCACATCGTGGCGCGTCTCCT GCTCAATTTCCAAAATACGGTCGATGTCAAAGTCCGCCTTCTTGCGAATCAAAGCCACAT CTTCCTTAGGGATTTCCCCCAACTCAGCCCATGCCTCGTCAGAGAGGATTTCCACCTCAA GCCAAGCACGGTATTTATTTTCTTCACTCCAAATATTCGCCATCTCAGGGCGAGAGTAAC GGTTGATCATGTGTTAATTTTTCCTTTCTTCTTAAGAT

ORF Predictions:

ORF # Start End Direction Length

1 930 1616 R 229 aa

>[SEQ ID NO:142] 3864180-2 ORF translation from 930-1616, direction R

VPKESLTQVLPRDLHAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSA

MPHKRNPIGSENMTGLARVIRGHMITAYENVALWHERDISHSΞAERIITPDTTILIDYML

NRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWD

NQVDFKPLLEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD*

Description:

ADENYLOSUCCINATE LYASE (EC 4.3.2.2) (ADENYLOSUCCINASE) (ASL) . - BACILLUS SUBTIL IS. Assembly ID: 3864184 Assembly Length: 4392bp

>[SEQ ID NO: 50] 3864184 Strep Assembly -- Assembly id#3864184

CCCTTTTGCCTCTCCCTTTGGTGCAGATTCTTTTGGGAATTGTGATTGGTCTCTTTTTAC

CCAATACTGACTTTCATCTTAATACGGAGTTGTTTTTGGCCTGGTTATCGGACCCTTGCT

TTTCCGAGAGGCTGAAGAAGCAGATGTTACGGCTATTTTAAAACACTGGCGAATCATTGT

TTATCTCATATTTCCAGTGATTTTTATCTCGACCCTGAGTTTGGGTGGCTTGGCCCATCT

TCTTTGGTTCAGCCTTCCCTTGGCAGCTTGCTTGGCTGTTGGGGCAGCCCTTGGTCCTAC

GGACTTGGTGGCCTTTGCCTCTCTTTCGGAGCGTTTTAGCTTTCCTAAGCGCGTGTCCAA

TATTCTTAAGGGCGAAGGACTCTTGAATGATGCTTCTGGTTTGGTGGCTTTTCAGGTAGC

TTTGACAGCTTGGACAACTGGAGCTTTTTCTCTGGGGCAAGCTAGCAGTTCGCTCATCTT

TTCAATCCTAGGCGGTTTTTTAATTGGATTTTTAACAGCCATGACCAACCGCTTCCTCCA

TACCTTCTTGCTAAGTGTGCGCGCAACGGATATTGCCAGTGAACTTTTATTAGAATTCGA

GTTTGCCTCTAGTGACCTTCTTTCTGGCAGAAGAAGTCCATGTTTCAGGGATTATTGCCG

TCGTAGTTGATCGAATTTTAAAGGCAAGTCGCTTCAAGAAAATCACGCTCCTCGAAGCCC

AAGTGGATACGGTGACCGAGACGGTCTGGCATACAGTGACCTTTATGCTCAACGGTTCTG

TCTTTGTGATTTTAGGGATGGAGTTGGAAATGATAGCAGAACCTATCTTGACCAATCCAA

TCTATAATCCTCTACTTTTATTGCTATCTCTCATCGCCCTTACCTTTGTCCTCTTTGTCA

TTCGTTTTATTATGATCTATGGCTATTATGCCTATAGAACCCGACGCCTAAAGAAAAAGC

TAAATAAGTATATGAAGGACATGTTTCTCTTGACCTTTTCAGGTGTTAAGGGAACGGTGT

CGATTGCTACGATTCTCTTGATACCAAGTAATCTAGAACAGGAGTATCCTCTCTTGCTTT

TCCTTGTTGCAGGTGTGACGCTTGTCAGCTTTTTAACAGGTCTCTTGGTCTTGCCTCATC

TTTCTGATGAAGAGGAAGAAAGCAAGGATTATCTCATGCATATCGCCATTTTGAATGAAG

TAACGCTAGAGTTGGAAAAAGAGTTGGAAGACACCAGAAATAAACTTCCCCTCTATGCGG

CTATTGACAATTCGATCATGGACGTATTGAAAATCTCATTTTAAGCCAAGAAAACCAGGA

TGATCAAGAAGACTGGGCTGCTTTGAAAATCGAATTCTTAGTATTGAAAGTGATGGTTTG

GAACAGGCCTATGAAGAGGGGAACATTAGCAATCGTGCTTACCGAGTTTACCAACGTTAT

CTGAAAAATATAGAACAAGGAATCAATCGTAAACTTGCCTCAAGACTGACCTATTATTTT

CTTGTTTCCTTGAGGATTTTACGTTTTCTTCTTCATGAAGTTTTTACTCTTGGAAAGACC

TTCCGTAGCTGGAAGGACAAGGAGCAAAGCCGTCTCCGTGCTCTTGATTATGACCAAATT

GCAGAGCTCTATCTTGCCAATACAGAGATGATTATTGAAAGTTTGGAAAACCTGAAGGGA

GTCTACAGACGCTCTTTGATTAGTTTTATGCAGGAGTCTCGTCTTCGAGAAACAGCTATT

ATCAGCAGTGGTGCCTTTGTCGAACGGGTTATCAATCGTGTCAAACCCAACAATATCGAT

GAAATGCTGAGAGGCTATTATCTGGAGCGCAAGTTGATTTTCGAATACGAAGAAAAACGA

TTGATTACGACTAAGTATGCCAAGAAATTACGACAAAATGTAAATAACTTAGAGAACTAT

TCCTTGAAGGAAGCTGCCAATACCCTGCCGTATGATATGGTGGAATTGGTAAGAAGAAAT

TAGTTAATACTCTTCGAAAATCTCTTCAAACCACGTCAGCGTCGCCTTGGATTATATATG

TGACTGACTTCGTCAGTTTCATCTACAACCTCAAAGCAGGGCTTTGAGCAACCTGCGGCT

AGCTTCCTAGTTTGCTCTTTGATTTTCATTGAGTATAAGATTGTAAGTGAAGGAGTGTGA

CATGAAAAAATGGGGAAAGAGCCTGAACTAGTCCTGTCTACTTTTACCCAATCACACTTC

CATTTGGTACAGCTGGATCAACTGTGAGAAGGGATCGAATTTGCCATCATGTTCAGCTGA

79

SUBSTTTUTE SHEET (RULE 26) GAGAATCATACCCTGGCTGACATATTTTTTCATCATTTTACGTGGTTTGAGGTTAGCAAC GATTTGAACTTTCTTGCCGACCAATTCTTGTTCATTTGGATAGTATTTTGCAATTCCTGA AAGAATCTGACGATCTTCTCCATCACCAGCATCCAAGCGGAATTGAAGCAACTTATCTGA ACCTTCTACTTTAGACACTTCTTTGACTTCTGCGACACGGATTTCAACCTTGTCAAAGTC TTCAAACTTGATTTCATCCTTGTTTAGTTTGAGCTCAACTTCGTCCGGATTCCATTCTTT TTCGACTGCTGGTTTATTGCCTTCCATTTGTTCCTTGATATAGGCGATTTCTTCTTCCAT ATTTAGACGTGGAAAGATAGGTGTTCCTTTGGCAACTACAGTCACATCTGCTGGGAAGTC AGCCAAACTCAAGTTTTCAAGACTAGAAACTTCTTCCAAACCAAGTTGAGTCAAAACTGC ACGACTAGTTTCCATCATAAATGGTTCAATCAAGTGAGCAACTACACGAATGCTGGCTGC CAAGTGGCTCATGACACTTGCCAATTGGTCACGAAGAGCTTCATCCTTGTCCAAGACCCA TGGTGCAGTCTCATCGATGTATTTATTGGTACGAGAGATCAGAGTCCAGACTGCTTCAAG CGCACGTGGATAGTCAACTGCTTCCATGTGTGTATGGAAGTCTGCGATTGATTTTTCTGC AACCTCAGCAAGAACATGATCAAATTCAGTCACACCTTCTACATAGGCAGGGATTTGTCC ATCAAAGTACTTATTAATCATGGAAACCGTACGGTTAAGGAGGTTCCCAAGGTCATTAGC CAATTCATAGTTGATACGACCGACATAGTCTTCAGGAGTAAAGGTTCCGTCTGAACCAAC TGGAAGGTTACGCATGAGGTAGTAACGAAGTGGATCTAGTCCATAACGCTCTACCAACAT TTCAGGGTAAACGACATTCCCTTTTGACTTAGACATTTTTCCGTCTTTCATGACAAACCA ACCATGGGCAATCAAACGATCAGGTAATTTAACATCCAACATCATAAGAAGGATTGGCCA GTAGATAGAGTGGAAGCGAAGGATGTCTTTTCCTACCATATGGAAGACTGTTCCATTCCA GAACTTGTCAAAGTTACCATGTTCGTCTTGAGCGTAGCCAAAAGCTGTCGCATAGTTAAG AAGGGCATCAATCCAAACGTAGACAACGTGTTTTGGATTTGATGGGACAGGCACTCCCCA TGTAAAGGTTGTACGAGATACCGCCAAATCTTCCAAACCTGGCTCGATGAAGTTGCGTAG CATTTCATTAAGACGACCATCTGGCGTGATAAATTCAGGATGAGCTTTGAAAAATTCGAC CAAACGGTCTTGGTATTTGCTAAGGCGAAGGAAGTATGATTCTTCAGAAACCCATTCAAC CTCATGACCTGATGGAGCAATACCACCAGTCACATTTCCAGCTTCATCACGGAAAACTTC TGCCAGCTGGCTTTCTGTAAAGAATTCTTCGTCTGATACTGAATACCAACCAGAGTATTC ACCCAAGTAGATATCATCTTGAGCAAGTAAGCGTTCAAAGACCTGTGCGACAACTTTTTC ATGGTAGTCATCGGTTGTACGGATAAATTTATCGTATGAGATATCTAGTAATTGCCAGAG TTCTTTAACTCCAACCGCCATTCCATCAACATAGGCTTGAGGTGTAATACCAGATTCGAA TTCCGCTTTCTGCTGGATTTTCTGACCATGTTCATCAAGACCTGTCAGATAAAATACATC GTAGCCCATCAGGCGTTTGTAACGTGCTAGGACATCACATGCGATAGTTGTGTAGGCAGA ACCGATATGAAGTTTCCCAGATGGATAGTAAATCGGCGTTGTAATATAAAAATTTTTTTC AGACATAATTTTTCCTTTCCAGGCAAATGAAACCTGTTTTTCTAACACTTCATTATATCA CATTTTTAATGAATTTCGATAGGGAAATCCATACCAAAACAAGATAGACGAGTGTCCATC TTGTTGATCTCATTCATAACGAAGGGCTTCAATTGGATCAAGTTTCGATGCCTTGTTGGC TGGCAAGACTCC

ORF Predictions:

ORF # Start End Direction Length

1 197 670 F 158 aa

2 612 1304 F 231 aa

80

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 143] 3864184-1 ORF translation from 197-670, direction F VIFISTLSLGGLAHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGE GLLNDASGLVAFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLS VRATDIASELLLEFEFASSDLLSGRRSPCFRDYCRRS*

Description: unknown

>[SEQ ID NO:144] 3864184-2 ORF translation from 612-1304, direction F

VTFFIAEEVHVSGIIAVVVDRILKASRFKKITLLEAQVDTVTETVWHTVTFMLNGSVFVI

LGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYYAYRTRRLKKKLNKY

MKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTLVSFLTGLLVLPHLSDE

EEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNSIMDVLKISF*

Description: unknown

Assembly ID: 3864194 Assembly Length: 1941bp

>[SEQ ID NO:51] 3864194 Strep Assembly -- Assembly id#3864194 AATTAGTATTCTCAACCTTTTTATCTTGATAGTTCAAGATGGCATTCGTTGAATTGGTAA CATAGTAACTATCCACTCCCTTCAGTTTAGCTGCCTCTTGAACCCAGGATTCTTGCGGTT TTGGCGGTTCAACAGGAATTCTTTTTCTTTTCCAGAAACCGTAAAAGCTGATTGTTTCTG AGTAAAAGACCCATCTTTACTTTTTTTAGGAGAGAAAAAGACGCTAATATTTTTCTGAGA TTTAGTCATATCTTTATTGACTTGACGAGATAGGGAATCACCCAAAGCCATAATCACAAC AACTGATGAAACACCGATAATAATCCCAATCATAGTAAGCAAAGAACGCATCTTGTGAGC CATGATAGATGAAAAGGCAAATTTCAGATTCTGCATCTTAGTTTTCCTCCTTTCCTAACT GAGCACTGTCAGACGAAATGACCCCATCCCGAATGACAATCTGACGTTTGGCATAGGCAG CAATCTCAGGCTTCATGCGTTACCATGATAATGGTTTTTCCTTCTTTATTCAAATCAACC AATAATTGCATAATTTGGTTACCTGTTTTGGTATCCAAGGCTCCTGTCGGTTCATCCGCT AGGATAATAGAAGGATTGTTTACCAAGGCACGCGCAATGGCTACACGTTGCTTTTGACCA CCAGATAATTCTGAAGGTAAATGGTGACTACGTTCTATCAATTCAACCTTGTCTAAATAT TCCTCAGCCAACTTGCGACGTTTTGAAGACGAAACTCCTGCGTAAATCAAGGGCAATTCT ACATTTTGCAGAGCATTGAGCTTCGATAGAAGAAAGAACTGCTGAAAGACAAAACCGATT TGTTGGTTACGGACCTTAGCTAGTTGTTTTTCACCAAGCCCAGCCACTTCTTGACCTTCA AGATAATATTCTCCACTGGTTGGTGTATCCAACATGCCAATCGTATTCATCAGAGTGGAC TTACCAGACCCAGATGGTCCCATGATGGCTACAAATTCACCCTCATTCACTTCTAGATTG ATATTTTTGAGAACCTGCAGTTCTTGGTCACCATTACGGTAACTTCTGAAGATATTTTTT AGACTAATTAGTTGCTTCATCAGCCTTCACCTCTTTTCCTTCTTCCAAGGAAGATGTTGG ATTACTGATGACCTTAGCACCGTTCGTTAAACCAGAAGTGATTTCTTGATTTTCTGCGTC AGCATTTCCCAATGAAACCTCAACTTTTTTAGCCTTTTGTTGTTCATCCACAATCCAGAC ATAATTTTTACTATCATCCATTACTAGACTGCTAACAGGAACAAGAATAGCCTTAGTTTT

81

SUBSTTTUTE SHEET (RULE 26) GCTTTTAACCTCAATGTTGACAGAAAAACCTTGTTTCAAATCACCAACCTCGCCTGTCAC ATCAATAGTATAAGGGTATTTAGAACCTGTATTATTCCCGGCTGCTGGACTAGCTGCTTC ACCATTGTTTTTAGGATAGTCAGAAATATAGGCTTAATTTCCCAGTCCATTTTTTATCAG GATACACTTTAGAAGTAAAGCTTACTTCTTGACCTACAGAAAGGTTGGCTAGATTGTACT CAGACAATTCTCCCTTGACTTGTAAATTTTCATTGCTGACAATATGAACCATAACTTGAC TCGCCCCTGTTGGAGATTTAGAAACATTGCTATTGACTTCGACTACAGTTCCCTCTAGGG TACTGAGAACAGTTGTTGCATCCAATTGACTTTGAGCCTTGCTTAATTGCGCTGCAGCAT CTGCACGCGCATCACGGGCATCACCCAATTGAGCATCAATAGAAGCAACAGAATTTCCAG CCACTGGAGTTGGGCTTTGCACCGTTGCATCTTCTCCTCCTACTGGCGCTGGTAACTGTG GAGCCTGAGCTGAAGCGGCTTCATTTCGTGCTTGATTGAGTTCATTGATATGACGATCTG CCTTAGCTACTGCTCGACTAG

ORF Predictions :

ORF # Start End Direction Length

1 1084 1380 R 99 aa

>[SEQ ID NO: 145] 3864194-3 ORF translation from 1084-1380, direction R

VTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLVMDDSKNYVWIVDEQQKAKKVEVSLGNA

DAENQEITSGLTNGAKVISNPTSSLEEGKEVKADEATN*

Description: unknown

Assembly ID: 3864338 Assembly Length: 1335bp

>[SEQ ID NO:52] 3864338 Strep Assembly — Assembly id#3864338

ATCGAATTCCCTATTTTAACACTTTCTTTTCTAAAACAGTCTATATTTTATTTCAAACTG

TATTATATTTTTGAAAAAATAAAGTCCTTTTTTCTTTTTTTCAGAAAAAAGGGTATAATA

AAAGAAAATAAGCAGTAACACTCAATGGAAATCGAAAAAGCAAACTAGGAAGCTAGCCGC

AGATTGCTCAAAACACTGTTTTGAGGTTGCAGATAGAGCTGACGTGGTTTGAAGAGATTT

TCGAAGAGTATAAAAAGGTGCTAGGCATGTTGATTTTTCCTTTGTTAAATGATTTGTCAA

GAAAAATCATCCATATTGGACATGGATGCCTTTTTTGCTGCAGTGGAAATCAGGGATAAT

CCTAAACTCAGAGGAAAACCTGTCATTATTGGAAGCGACCCTCGGCAAACAGGTGGACGG

GGAGTCGTTTCTACCTGTAGTTATGAGGCAAGAGCTTTTGGTGTCCATTCTGCCATGAGT

TCCAAGGAAGCTTATGAACGTTGTCCCCAGGCTGTCTTTATCTCAGGGAATTCGATGAGA

AATACAAGTCTGTGGGACTCCAGATTCGAGCTATTTTTAAGCGCTATACAGATTTGATTG

AACCCATGAGCATTGACGAAGCCTATTTGGATGTGACAGAAAATAAACTCGGTATCAAGT

CAGCGGTCAAAATTGCTCGCCTCATTCAAAAAGATATCTGGCAAGAACTCCATCTAACTG

CTTCCGCAGGCGTTTCTTACAACAAATTCTTAGCTAAAATGGCGAGTGATTATCAAAAAC

CACATGGTTTGACAGTGATTCTACCTGAACAGGCTGAGGATTTTCTCAAACAAATGGATA

TTTCCAAATTTCATGGAGTAGGAAAAAAGACAGTAGAACGTCTTCATCAAATGGGCGTTT

82

SUBSTTTUTE SHEET (RULE 26) TTACTGGTGCTGATTTACTTGAAGTTCCTGAGGTAACCCTAATAGACCGTTTTGGTAGAC TAGGCTATGATCTGTATCGAAAGGCTCGTGGCATTCACAACTCTCCAGTCAAATCCAATC ACATCCGTAAATCAATCGGCAAGGAGAAAACCTACGGGAAGATTCTCCGTGCTGAGGAAG ATATCAAAAAAGAGAGCTGACTCTTCTATCAGAAAAAGTCGCTCTCAATCTACATCAACA AGAAAAAGCTGGAAAAATTGTCATTTTGAAAATCCGCTACGAGGACTTTTCAACTCTTAC CAAACGAAAAAGTATTGCTCAAAAAACACAAGATGCTAGTCAGATAAGCCAAATAGCCCT GCAACTCTATGAAGAATTAAGTGAGAAAGAAAGAGGTGTCCGCCTATTGGGGATTACCAT GACTGGATTTTAAAG

ORF Predictions :

ORF # Start End Direction Length

1 552 1100 F 183 aa

>[SEQ ID NO: 146] 3864338-2 ORF translation from 552-1100, direction F

VGLQIRAIFKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAG

VSYNKFLAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGA

DLLEVPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNHIRKSIGKEKTYGKILRAEEDIKK

ES*

Description:

ECODINJ NCBI - Escherichia coli (sub_strain W3110, strain K-12) DinP, DNA damage inducible protein

Assembly ID: 3864360 Assembly Length: 1796bp

>[SEQ ID NO:53] 3864360 Strep Assembly -- Assembly id#3864360

TCCAAGCTAGCTATTTCGTGGAAGGGGCTTCGGTTGGCAGAACCTGGTGAATTTACCCAA

ACGTGCTTTTTTAAACGGTCGCGTAGACTTGACACAGGCAGAGGCTGTGATGGATATCAT

CCGTGCCAAGACTGACAAGGCCATGAACATTGCGGTCAAACAATTAGACGGCTCCCTTTC

TGACCTCATTAACAATACCCGTCAAGAAATCCTCAATACACTTGCCCAAGTTGAGGTCAA

TATCGACTATCCTGAATATGATGATGTTGAGGAAGCTACTACTGCCGTTGTCCGTGAGAA

GACTATGGAGTTTGAGCAATTGCTAACCAAGCTCCTTAGGACAGCACGTCGTGGTAAAAT

CCTTCGTGAAGGAATTTCAACGGCTATCATTGGACGTCCCAACGTTGGGAAATCAAGCCT

TCTCAACAACCTCTTGCGTGAGGACAAGGCTATCGTAACCGATATCGCTGGGACAACACG

AGATGTCATCGAAGAGTACGTCAACATCAATGGTGTTCCTCTAAAATTGATTGACACAGC

TGGTATTCGTGAAACGGATGATATCGTTGAACAAATCGGTGTTGAGCGTTCGAAAAAAGC

CCTCAAGGAAGCCGACTTGGTTCTACTAGTGCTAAATGCCAGTGAACCACTGACTGCGCA

AGACAGACAACTTCTTGAAATTAGCCAAGATACCAATCGCATTATTCTACTTAATAAAAC

CGACCTGCCAGAAACGATTGAAACTTCGAAACTACCTGAAGACGTTATCCGTATTTCAGT

CCTTAAAAACCAAAACATCGACAAGATTGAAGAGCGAATCAACAACCTCTTCTTTGAAAA

TGCTGGCTTGGTCGAGCAAGATGCTACTTACTTGTCAAACGCCCGTCACATTTCCCTGAT

83

SUBSmUTE SHEET (RULE 26) TGAAAAAGCAGTTGAAAGCCTACAAGCCGTTAATCAAGGTCTTGAGCTGGGGATGCCAGT TGATTTGCTTCAAGTTGACTTGACTCGTACTTGGGAAATCCTCGGAGAAATCACTGGGGA TGCTGCTCCAGATGAACTCATCACCCAACTCTTTAGCCAATTCTGTTTAGGAAAATAAGA AAAATCCATGATCCTTCATTCGGTCATGGATTTTATTGTCTTTATTAGTAATCTGGTCTT AAGACCCCTGTTACAGTTGCCTTAGTTGCTTCGTAGTCGCCATCTACGACAACCTTGATA ATGCGTTTGACATCTTCTTCTGGTGCTGGAACAAGAGGTAGACGAGTGGGTCCAGCTTCA AATCCCATATAGTTAAGAATTGCCTTAACTGGAGCAGGACTTGGATAAGAGAAGAGAGCA TTAACCTTAGGAATGAATTTACGCTGAATTGCTGCGGCTTTCTTCATATCGCTTTCTGCA ATGGCAGTAAACATCTCGTGCATTTCATCCCCATTTGTATGAGAGGCAACAGAAATAACC CCATCCGCCCCAAGGTTCATGGCATGGAAAGCATCTCCATCCTCACCTGTATAAATCAAG AACTCTTCAGGCTTGTGCTCAATCAAGTAAGCCATATTAGCCAAGCTAGTACATTCTTTG ACACCGATAATATTTGGATGGTCAGCCAAGCGAAGCATGGTTTCTGGAGTCAATTCGACA ACTACACGCCCTGGAATGTTATAGATAATAATTGGTAGGTCAGAAGCATCTGCAATAGCC TTAAAGTGCTGATACATCCCTTCTTGAGAAGGTTTGTTGTAGTAAGGAACAATAGCAAGC CCAGCTGCGAAACCACCAAATTCCGCTACTTCTTTGACAAACTCAATAGAGTCACG

ORF Predictions:

ORF # Start End Direction Length

1 47 1078 F 344 aa

>[SEQ ID NO: 147] 3864360-1 ORF translation from 47-1078, direction F

VNLPKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSDLINNTRQEILNTLA

QVEVNIDYPEYDDVEEATTAWREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNV

GKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVE

RSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRIILLNKTDLPETIETSKLPEDV

IRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLE

LGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK*

Description: THIOPHENE AND FURAN OXIDATION PROTEIN THDF . - ESCHERICHIA COLI .

Assembly ID: 3864388 Assembly Length: 2337bp

>[SEQ ID NO: 54] 3864388 Strep Assembly -- Assembly id#3864388

CTTCGTACAGGTGGTTCCTATGCAAGGGTGGAAGCCAATCGTCAGAACAACAAGCATCTT

CATCAAGCCAGAACTGGAGCAATTACAAAAAGAAATTGCTGAAGAAGAAGCAAGCTTGGG

TTCAGAAGAAGTGGCTTTGAAGACCTTGCAAGATGAGATGGCCAGATTGACCGAGTCATT

AGAAGCTATTAAATCTCAAGGAGAGCAGGCACGTATTCAGGAGCAAGGCTTGTCCCTCGC

TTATCAGCAAACTAGTCAGCAAGTTGAAGAACTGGAAACTCTTTGGAAACTCCAAGAAGA

GGAAATAGATCGTCTTTCCGAGGGAGATTGGCAAGCGGATAAGGAAAAATGCCAAGAGCG

TCTTGCTGCAATCGCCAGTGACAAGCAAAATCTGGAAGCTGAGATTGAAGAGATTAAGTC

84

SUBSTTTUTE SHEET (RULE 26) TAATAAAAATGCCATCCAAGAACGCTATCAAAACTTGCAGGAAGAGCTAGCGCAAGCTCG TTTGCTTAAGACAGAACTGCAAGGGCAAAAACGTTATGAAATTGCTGATATTGAACGCTT AGGCAAGGAATTGGACAATCTTGATTTTGAACAAGAGGAAATCCAGCGCCTTCTTCAAGA AAAGGTTGACAATCTTGAGAAGGTTGATACAGAATTGCTCAGTCAACAGGCGGAAGAATC CAAAACTCAGAAAACGAACCTCCAACAAGGTTTGATTCGCAAACAGTTTGAGTTGGATGA TATAGAAGGTCAGCTGGATGATATTGCTAGTCATTTGGATCAGGCTCGCCAGCAGAATGA GGAGTGGATTCGCAAGCAAACACGTGCTGAAGCTAAGAAAGAAAAGGTCAGCGAGCGCTT TGCCGCCATCTACAAAGTCAATTAACAGACCAGTACCAGATTAGCCATACTGAAGCTCTA GAAAAAGCGCATGAATTGGAAAACCTCAATCTGGCAGAGCAAGAAGTTAAGGATTTAGAG AAGGCTATTCGCTCACTGGGTCCTGTCAATATAGAAGCTATTGACCGGTACGAAGAAGTT CACAACCGTCTGGACTTTCTAAATAGTCAGCGAGATGATATTTTGTCAGCGAAAAATCTG CTCCTTGAAACCATTACAAAGATGAATGATGAGGTTAAGGAACGCTTTAAATCAACCTTT GAAGCTATTCGTGAGTCCTTTAAAGTGACCTTCAAGCAGATGTTTGGCGGAGGTCAGGCA GACTTGATATTGACTGAGGGCGACCTTTTACAGCTGGTGTGGAGATTTCTGTTCAACCTC CAGGTAAGAAAATCCAGTCGCTTAACCTCATGAGTGGTGGTGAAAAAGCCCTATCGGCTC TTGCCTTGCTTTTCTCCATTATTCGTGTCAAGACCATTCCTTTTGTCATCTTGGATGAGG TGGAAGCTGCGTTGGATGAAGCCAATGTTAAACGTTTTGGGGATTACCTCAACCGCTTTG ACAAGGACAGCCAGTTTATCGTCGTAACCCACCGTAAGGGAACCATGGCAGCGGCCGATT CCATCTATGGAGTGACCATGCAAGAATCGGGTGTTTCAAAGATTGTTTCAGTTAAGTTAA AAGATTTAGAAAGTATTGAAGGATGACAATTAAACTAGTAGCAACGGATATGGACGGAAC CTTCCTAGATGAGAATGGGCGCTTTGATATGGACCGCCTCAAGTCTCTCTTGGTTTCCTA CAAGGAAAAAGGGATTTACTTTGCGGTGGCTTCGGGTCGGGGATTTCTGTCTCTGGAAAT CGAATTATTTGCTGGTGTTCGTGATGACATTATTTTCATCGCGGAAAATGGCAGTTTGGT AGAGTATCAAGGTCAGGACTTGTATGAAGCGACTATGTCTCGTGACTTTTATCTGGCAAC TTTTGAAAAGCTGAAAACGTCACCTTATATAGATATCAATAAACTGCTCTTGACGGGTAA GAAGGGTTCATATGTTCTAGATACGGTTGATGAGACCTATTTGAAAGTGAGTCAGCATTA TAATGAAAATATCCAAAAAGTAGCGAGTTTGGAAGATATCACAGATGACATTTTCAAATT TACAACCAACTTCACAGAAGAAACGCTAGAAGCTGGTGAAGCTTGGGTCAATGATAATGT CCCTGGTGTCAAGGCTATGACAACTGGCTTTGAATCTATTGATATTGTTCTGGACTATGT CGATAAGGGTGTAGCTATTGTTGAATTAGCTAAAAAACTTGGCATCACAATGGATCAGGT CATGGCTTTTGGAGACAATCTTAATGACTTACATATGATGCAGGTTGTGGGACATCCTGT AGCTCCTGAAAATGCACGACCAGAGATTTTAGAATTAGCATAAGACTGTGATTGGTC

ORF Predictions:

ORF # Start End Direction Length

1 1239 1586 F 116 aa

>[SEQ ID NO:148] 3864388-3 ORF translation from 1239-1586, direction F

VEISVQPPGKKIQSLNLMSGGEKALSALALLFSIIRVKTIPFVILDEVEAALDEANVKRF

GDYLNRFDKDSQFIWTHRKGTMAAADSIYGVTMQESGVSKIVSVKLKDLESIEG*

Description: P115 protein - Mycoplasma hyorhinis (SGC3) (similarity to SMC1_YEAST, chromosome segragation protein)

Assembly ID: 3864406 Assembly Length: 2162bp

>[ΞEQ ID NO: 55] 3864406 Strep Assembly -- Assembly id#3864406

CTAAAAGTGAAGCCCGATAGCGTCTCTCTCCTGCAAGGATTTCATAACCAATAACAGGAG

ATTGACGAACAATAATCGGTTGAATGACCCCATTTTCTTTGATAGACTGTGCTAGTTCAT

CTAGCTTTTCTCTATCAAATTCTTTTCGGGGTTGATAGGGATTTTTTTGTATATCTGTGA

TAGAAATCATTTCAAATTTTTCCATGATTCTACACTAACACATCTTTTCTCTTATGTAAA

GCTTTCTTTACATAGATGTCAATTAAGATTCTAAATCACCTGAACTCTTGTTAAGTTTGA

TAGAGGTAGTTTCTTCTTTCCCGTTACGATAGTAGGTTATCTTAATGGTGTCTCCGATAG

AATGGTTGTAAAGAGCACTTTGTAAGTCTGTTGATGAAGCAATCTCTTTGTCATCTACTT

TTGTAATTACATCGTATTTTTCAAGGTGACCATTGGCAGGCATATTACTTTGTACCGAAC

GAACAATTACACCAGATGTAACATTACTTGGAATATTGAGTCTTCTGATGTCGCTTGTAC

TCACATTAGATAAATTAACCATCTGGATTCCCAAAGCTGGACGCGTCACTTTTCCGTTTT

TTTCTAACTGTTCAATAATATTGATAGCATCATTTGCAGGAATTGCGAAACCAAGACCTT

CTACAGATGTTCCTCCATTTGTAGCAATTTTACTTGAGGTAATTCCGATAACCTGCCCTT

GAATATTGATCAGTGGGCCGCCAGAGTTACCTGGGTTAATAGCAGTATCAGTTTGGATGG

CTTTTGTAGAAATAGCTTGTCCATCTTCCGATTTTAAGGATACATTTCTATTGAGACTGG

ATACGATACCTTGAGTGACAGTATTTGCATATTCAGAACCTAACGGGCTACCGATGGCAA

TAGCAGTTTCTCCTACAGTTAACTTACTAGAATCACCAAACTCAGCTACTGTTGTCACTT

TTTCTGAAGAGATTTCGACGACAGCAATATCAGAGAAAGTGTCAGCTCCGACAATTTCTC

CAGGTACTTTAGTCCCATCTGACAATCGAATATCTACTTTGCTGGCGCCATTTATAACGT

GATTGTTGGTGACGATGTAAGCTTCTTTATCATTCTTTTTATAAATAACTCCAGATCCTT

CACTAGAGATTCGCTGAGAATCTGTGTCAGTATCATCATTGCCAAATACGCTATTTTGTC

TGTTTGCCGAATAAGTAATAACAGAAACAACAGCATCTTTTACTTTGTTAACGGCCTGTG

TTGTTGAATTTTCCGTTCCTTATAGGCAGTTTGTGTAATAGTACTATTGTTGTTAGAGTT

GTTTACACTACTTTTTTGAGTTAGTTGAGTTATTGAAAAACTACCCAAGGCTCCACTAAA

AAAGCTAATGACGATAACGACTAATAATTGAAACCATTTTTTGTAAAATGTTTTTAGATG

TTTCATATTTGCCTCCATATGTTTGAATTACTGAAAGTATAAACTGACTAGCTTAATTAT

AACTTAAACACAAAAGTTTTACACAAACTGTGGATAACTCTTTTGAAACTGTGATTTTCT

TAATTGAAATCTATTTTTTATTTTGTGAATAAGATGTGAAAAAATAGAGAATATGTTAGA

ATAGAGTCATGAAAATTAAAGTTGTAACAGTTGGGAAACTGAAAGAAAAGTATTTAAAAG

ATGGTATCGCAGAGTATTCAAAACGAATTTCTAGATTTGCTAAGTTTGAAATGATTGAGT

TATCAGATGAAAAAACACCAGATAAGGCCAGTGAATCAGAAAATCAAAAGATTTTAGAAA

TAGAAGGTCAGAGAATTTTATCAAAAATTGCTGACCGTGATTTCGTTATTGTGTTAGCCA

TTGAAGGGAAAACTTTCTTCTCAGAAGAATTTAGTAAGCAGTGAGAAGAAACTTCTATAA

GGAAGGATGTCTACTCTTACTTTTATTATTGGGGGAAGTTTAGGATTGTCATCATCTGTA

AAAAATAGAGCCAATCTTTCTGTCAGTTTTGGTCGCCTAACCTTGCCTCATCAGTTAATG

AGACTAGTTCTTGTTGAACAAATCTATCGCGCTTTTACGATTCAGCAGGGATTCCCCTAC

CATAAATAGAGAATTGACTTTTAATTGAATTTTTGGTAGAATAATTGTGTTAGGTCTCAT

86

SUBSTTTUTE SHEET (RULE 26) AG

ORF Predictions :

ORF # Start End Direction Length

1 263 958 R 232 aa

>[SEQ ID NO: 149] 3864406-1 ORF translation from 263-958, direction R VTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKA IQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEK NGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHLEKYDVITK VDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES*

Description:

Bacillus subtilis (strain 168, ) DNA. Homologous to E. coli serine protease HtrA (BLAST)

Assembly ID: 3864452 Assembly Length: 1766bp

>[SEQ ID NO: 56] 3864452 Strep Assembly -- Assembly id#3864452 ATCGAATTTTCCAAAATGGGGAGCTAGAGCAGTGGAGTGATTATGTGGCAGACGATTTGA TTCAGCATAATCATGAGATTGGACAAGGAAGTGCTGCTTATAAAAACTATGTGGCTGAAT ATATTGTCACTTTTGACTTCGTTTTCCAACTCTTAGGACAAGGAAACTATGTGGTTAGCT ATGGTCAGACTCAGATTGATGGCGTTGCTTATGCCAAGTACGATATCTTCCGTTTAAAGA ACGGGAAAATTGTGGAGCATTGGGATAATAAGGAAGTCATGCCTAAGGTAGAAGACTTGA CCAATCGAGGGAAGTTTTAAATTGAGGACAAAGAATGATTGAATACAAAAATGTAGCACT GCGCTACACAGAAAAGGATGTCTTGAGAGATGTCAACTTACAGATTGAGGATGGGGAATT TATGGTTTTAGTAGGGCCTTCTGGGTCAGGTAAGACGACCATGCTCAAGATGATTAACCG TCTTTTGGAACCAACTGATGGAAATATTTATATGGATGGGAAGCGCATCAAAGACTATGA TGAGCGTGAACTTCGTCTTTCTACTGGTTATGTTTTACAGGCTATTGCTCTTTTTCCAAA TCTAACAGTTGCGGAAAATATTGCTCTCATTCCTGAAATGAAGGGGTGGAGCAAGGAAGA AATTACGAAGAAAACAGAAGAGCTTTTGGCTAAGGTTGGTTTACCAGTAGCCGAGTATGG GCATCGCTTACCTAGTGAATTATCTGGTGGAGAACAGCAACGGGTCGGTATTGTCCGAGC TATGATTGGTCAGCCCAAGATTTTCCTCATGGATGAACCCTTTTCGGCCTTGGATGCTAT TTCGAGAAAACAGTTGCAGGTTCTGACAAAAGAATTGCATAAAGAGTTTGGGATGACAAC GATTTTTGTAACCCATGATACGGATGAAGCCTTGAAGTTGGCGGACCGTATTGCTGTCTT GCAGGATGGAGAAATTCGCCAGGTAGCGAATCCCGAGACAATTTTAAAAGTGCCTGCAAC AGACTTTGTAGCAGACTTGTTTGGAGGTAGTGTTCATGACTAATTTAATTGCAACTTTTC AGGATCGTTTTAGTGATTGGTTGACAGCTACAATGACATTGGTCGGTTCCTTGAGCAAGA GATAGATTAGCCAGACAGTCATGCCCAAAATCCCTCCAGGTAAGAGCATAGACCGTTGCA CATTAAGTACGATTAAAAAAGTGATAATGGCAAGAAAACTTGCTACTGCTTGTAATAAAA AGGTTGTTAGTGTCATATTAGTTCATCAATACCAAGGCGACAGAAGTTCCTGCCCCTAAA

87

SUBSTTTUTΈ SHEET (RULE 26) GCGAGGGTAATGAGCAGGGATTCAAACATCTTACTCATACCAGAGTTTATGTGGTTGGTC ATAATATCACGGACCGCATTGGTCAAGGCAATACCTGGTACAAACGGCATGACCGCACCA GCTATAATCAAATCTGCCGTTGAAGGAAAACCTGTGTAGCGAGCCCAAAACTGGGCAATT ATCCCAAAGACAAAAGCTCCAGCAAAGGCTGTCACAAAGGGAATTCGGATAAATTTTTCC ACATAGAGGGAAAAGGCAAAACCAAATAAGGTCGCCACTCCTGCCCCAAGTGCGTCGTAG ATATTTCCGCTAAACATAACTGAAAAGAAAGGAGCACTAAAGGTCGCAGCCAGAGTTACC TGCAACTTAGTATAGGGAAGGGGTTGAGCTTGCAAGGCCGTCAATTGCTTAAAGGCTGTT TCTAAGTCAATCTGCCCCCCAACTGG

ORF Predictions :

ORF # Start End Direction Length

1 1079 1201 R 41 aa

>[SEQ ID NO: 150] 3864452-2 ORF translation from 1079-1201, direction R VQRSMLLPGGILGMTVWLIYLLLKEPTNVIVAVNQSLKRS*

Description: unknown

Assembly ID: 3864458 Assembly Length: 1705bp

>[SEQ ID NO: 57] 3864458 Strep Assembly -- Assembly id#3864458

CTCTGACGGAGGCTGGTTATGTGGGTGAGGATGTGGAAAATATACTCCTCAAACTCTTGC

AGGTTGCTGACTTTAACATCGAACGTGCAGAGCGTGGCATTATCTATGTGGATGAAATTG

ACAAGATTGCCAAGAAGAGTGAGAATGTGTCTATCACACGTGATGTTTCTGGTGAAGGGG

TGCAACAAGCCCTTCTCAAGATTATTGAGGGAACTGTTGCTAGCGTACCGCCTCAAGGTG

GACGCAAACATCCACAACAAGAGATGATTCAAGTGGATACAAAAAATATCCTCTTCATCG

TGGGTGGTGCTTTTGATGGTATTGAAGAAATTGTCAAACAACGTCTGGGTGAAAAAGTCA

TCGGATTTGGTCAAAACAATAAGGCGATTGACGAAAACAGCTCATACATGCAAGAAATCA

TCGCTGAAGACATTCAAAAATTTGGTATTATCCCTGAGTTGATTGGACGCTTGCCTGTTT

TTGCGGCTCTTGAGCAATTGACCGTTGATGACTTGGTTCGCATCTTGAAAGAGCCAAGAA

ATGCCTTGGTGAAACAATACCAAACCTTGCTTTCTTATGATGATGTTGAGTTGGAATTTG

ACGACGAAGCCCTTCAAGAGATTGCTAATAAAGCAATCGAACGGAAGACAGGGGCGCGTG

GACTTCGCTCCATCATCGAAGAAACCATGCTAGATGTTATGTTTGAGGTGCCGAGTCAGG

AAAATGTGAAATTGGTTCGCATCACTAAAGAAACTGTCGATGGAACGGATAAACCGATCC

TAGAAACAGCCTAGAGGTGACTATGGAACTTAATACACACAATGCTGAAATCTTGCTCAG

TGCAGCTAATAAGTCCCACTATCCGCAGGATGAACTGCCAGAGATTGCCCTAGCAGGGCG

TTCAAATGTTGGTAAATCCAGCTTTATCAACACTATGTTGAACCGTAAGAATCTCGCTCG

TACATCAGGAAAACCTGGTAAAACCCAGCTCCTGAACTTTTTTAACATTGATGACAAGAT

GCGCTTTGTGGATGTGCCTGGTTATGGCTATGCTCGTGTTTCTAAAAAGGAACGTGAAAA

GTGGGGGTGCATGATTGAGGAGTAATTTAACGACTCGGGAAAATCTCCGTGCGGTTGTCA GTCTAGTTGACCTTCGTCATGACCCGTCAGCAGATGATGTGCAGATGTACGAATTTCTCA AGTATTATGAGATTCCAGTCATCATTGTGGCGACCAAGGCGGACAAGATTCCTCGTGGTA AATGGAACAAGCATGAATCAGCAATCAAAAAGAAATTAAACTTTGACCCAAGTGACGATT TCATCCTCTTTTCATCTGTCAGCAAGGCAGGGATGGATGAGGCTTGGGATGCAATCTTAG AAAAATTGTGAGGAAAAGAAAATGGCAAAAACAATTCATACAGATAAGGCCCCAAAGGCT ATCGGGCCCTATGTTCAAGGAAAAATCGTTGGCAACCTTTTGTTTGCTAGCGGTCAAGTT CCCCTATCCCCTGAAACTGGGGAAATTGTAGGAGAGAATATCCAAGAACAGACAGAGCAA GTCTTGAAAAACATCGGTGCTATTTTGGCAGAAGCAGGAACAGACTTTGACCATGTTGTC AAAACAACTTGTTTCTTGAGCGATATGAACGACTTTGTTCCTTTTAATGAGGTTTACCAA ACGGCCTTCAAAGAGGAATTCCCAG

ORF Predictions:

ORF # Start End Direction Length

1 797 1105 F 103 aa

2 1179 1391 F 71 aa

>[SEQ ID NO: 151] 3864458-2 ORF translation from 797-1105, direction F

VTMELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINTMLNRKNLARTSGKP

GKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEE*

Description: unknown

>[SEQ ID NO:152] 3864458-3 ORF translation from 1179-1391, direction F

VQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESAIKKKLNFDPSDDFILFSSVSKAGMD

EAWDAILEKL*

Description:

HYPOTHETICAL 22.0 KD PROTEIN IN LON-HEMA INTERGENIC REGION (ORFX). - BACILLUS S UBTILIS.

Assembly ID: 3864474 Assembly Length: 1673bp

>[SEQ ID NO: 58] 3864474 Strep Assembly -- Assembly id#3864474

ACGTTTTGGGAACTGTTCGGATAGCAGATTCCGAACAAACTGATAATGGTTGGCAAAATC

ATTATTCCTAATAGTAACGAAGCTGGTTAGGACAACTCATGCCATTTCCTAAAAAGGTTT

TAATCCAAGGCACCAATAATTGTAGGCCGAAAAAACCATAAACAATAGATGGAATGGCTG

CCATCAAGTTGATAGCTGATTTTAAGAAGCTATAGACGGGCTTTGGACAATTATAAACCA

TAAACACCGATGTCAAGATCGCCTGTTGGCACCCCAATCACAATCGCTCCTAAGGTCGAA

TAAATAAGGAACCAACGATCATTGGTAAAATACCATAGCTTGCCGGAATGTTCGTTGGCG

ACCAATCACTGCCTAATAAAAAACGGGCAAAGCCGTAGTTAGCTATGAAAGGTAAGCCAT

89

SUBSTTTUTE SHEET (RULE 26) TACTAAAAATAAAGAAACAGATTAGCAAAATAGCTACAACAGCTACTGTTGCACTCATGA AAAAAATTGCCCTAAAAACTGCTTCTTTGAAGGCTTGTTTTGTCACATCTTGTCCTTTCT AGTGAAGAAAGTAAGGGAGATACGACACCTCCCTACTTGCCTTCTTTATCTTATTGTACG ATGAAACGTCTGCATCTCTTTAGAGATTTATGGAGCAAACATTTTATTTAATCTTGTCCC AGGTGGTTAATTTGCCACTAAAAACGTCCGCAAGTTCAGCCATACTGACTTGGCTTGCCT TATTGTCATTATTGACCACAACAGCAATACCGTCTAAAGCAATAGCATCATGGGTGAGAC TCTTACCTTCTTCAGGAGTTAATTCCCTAGAAACCATACCAATATCAGCGGTTTTCTCCT TAACAGCGGTAATACCTGCTGAAGACCCATTAGAGGTAATATCAATCGTAACTTCTGGAT TTTCTTTTTTATAAGCTTCTGCTAATTTTTCCATTAAAGAAGATACTGAAGTGGAACCTA CAACAGACAACTTGCCTGATAAGTGTTGGCTTGTATATTCTGTGGTTTCGGTTTTAGCTT CAATAAATTTATTATCTGTGACCACTTGTTGACCTTGTTTGGAGTGGATAAAGCTGATAA AATCTTGACCTAGCTTGGAAAGATTAGAAGACCAAACAATGTTGAAGGGACGTTGAAGAG GGTATTCACCATCTAAAACTGTGTCTCGACTAGCCTTGACACCATCAATCTCTAAAGCCT TGACAGATTTCGTTAAAGATCCCAAGGAGATGTAGCCGATAGCATTAGCATTCCCTTGAA CTGCTGAGAGAACACCTTCTGTACTATTTTGAATCACAGCTGTTTTGGCAGTGTAGTCAA TTTTTTTATCACCGTCTTTTTTGAGAATCCCTGTGATTTCTGTGAAGGCACCCCGTGTTC CAGAGCCATTTTCTCGTGAAATCACCTCAATCGTTCCTGGAGCTGACTGTTTGGAAGCAG CTGACTGATTGCCACAGGCAACAAGCCCAAATCCTGATAAGCCAATGGCTGCAAGAGTAA GCATTTTTTTGAATTTCATAATAATCACCTTTATCTCTATGTATTTTTCTTGTGTAGGCT TACTACATTTATAGTCTAACAAGTCTTTGTAAAGGTTTATCCCTGATTCATGTAAAGATT GTGTAAAGAATCAAAAAAAGCCACTTTTGAAAAATGGCTGCCCCTAAAAATAG

ORF Predictions :

ORF # Start End Direction Length

1 68 247 R 60 aa

2 644 1528 R 295 aa

>[SEQ ID NO: 153] 3864474-1 ORF translation from 68-247, direction R VFMVYNCPKPVYSFLKSAINLMAAIPSIVYGFFGLQLLVPWIKTFLGNGMSCPNQLRYY*

Description:

PROBABLE ABC TRANSPORTER PERMEASE PROTEIN (ORF72) . - BACILLUS SUBTILIS. (BLAST)

>[SEQ ID NO:154] 3864474-2 ORF translation from 644-1528, direction R

VIIMKFKKMLTLAAIGLSGFGLVACGNQSAASKQSAPGTIEVISRENGSGTRGAFTEITG

ILKKDGDKKIDYTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSLTKSVKALEIDGVKAS

RDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQWTDNKFIEAKTETTEYTS

QHLSGKLSWGSTSVSSLMEKLAEAYKKENPEVTIDITSNGSSAGITAVKEKTADIGMVS

RELTPEΞGKSLTHDAIALDGIAWVNNDNKASQVSMAELADVFSGKLTTWDKIK*

Description:

90

SUBSTTTUTE SHEET (RULE 26) probable hemolysin precursor - Streptococcus agalactiae (strain 74- 360)

Assembly ID: 3864510 Assembly Length: 1702bp

>[SEQ ID NO:59] 3864510 Strep Assembly -- Assembly id#3864510

CTTTTTTATTTCACAACAAGTTCATAACGTGTCTTACTGGTGAAGGTTTGACCAGCTTTA

AGAATGACTTGGCCTTTAAGGTCACTGTGAATGGCATCTGGTAAAGCTTGCGCTTCAAGA

GCAATCCCATTGTGCTGTAGCATTGGCTGACCTCCTATGATGACACTTTCATCCACAAAG

TTTGCTGTGTAGACCACAAAGCAAGGAGCTTCTGTCTTGAAAAGCAGGAAGCGACCTGAA

TTTTGGTCATAAAGGAATCCAGCATTGTCATGGCCTGCAGGAAGGGCAAATGGATGATCC

AAACCTGATGCCAGCTGGATTTGCTCATCTTCTTCTGCAAAGATATCCTTCAACAAGGCA

CCATTGTAGATGTGTTTGACCACATCACGGTTGGCTTCTGGAGTTTTGGCAGGAACACCG

TCAGGAGCGATTGAGTAAATGCCCTCTGTGTTTAGTTGGAAGACATGACGGTCAATCGTC

TGCGTGAAATCACCAGACAAGTTGAAATAGCTGTGGTTGGTTGGATTGACCAGCGTATCC

TGATCGGTCGTTACCTTGTAGATCGAATTCATGGAGGCACCAGTTTCTTCCAAGTGATAA

CTGATCGCCAAATCTTGAGATTTCCAGGGAACCCTCCTGTCCCATCTGTACGCTCTGTGT

AGAGAGTCAAGCCATGATCGCTTACTTCTTCAACTTCAAACAAGCTGGAATCCCAACCAG

TTGAACCACTGTGATTACAGTTGCTAGCATTATTAACCTCAAGGTCATAGGTCTTACCAT

TGAGCTCAAAGGTCGCACCTGCAATACGACCCGCTACAGGACCTACACTTGCTCCATGCT

TGGGACTATTGCCTACATAACTATCAAAGTCATCAAATCCCAAGATAACATTGGCAAAAT

TTCCAGCCTTGTCAGGTGCGACATAGCGCAAGATAGTCGCACCATAAGTCATAACCTCAA

GTTGGTAGCCACCGTCTGTCTCAAATCGATAGGCCAAGACATCCTCACCCTCAACATTTC

CAAATACACGCTCTGTGTATGCTTTCATTCTGTTCTCCTTTTACTATTTCTCTCAAGCAA

ACAAACCATAGAAAGCGTACTGACAATCTATGGTTTATCTGATAATTTACAAATCCTCTT

GTCAAGAATTCATAAACACTGTCTTACTTTTGATATTCGTGAATTATGACACCTTGTACT

ACACGGTTTACTGTACCTGTAGGAGACGGTGTATCTGGTTTATTTTCTACCTTGAGTGAA

GTCAATAGGGCAAAGAGTTGGGCATAAACGATGTAAGGGAAGACACGGTAAATATCATTC

AAGACACCGCCACAACCAAGGGCCACTTCTTTGACATTTTCAAGACCAAAAGCTTGATCA

CTCAAAAGCACAACACGACGAGCAATCTGGTCACCAGCAACTTCACGAACCAAGTCCAAG

TCGTACTTACGAGTGTAGTCCGTCGTTGTACCAAAGACCAAAACAACTGTATTGTCGTTG

ATAAGAGATTTTGGACCGTGACGGAAGCCAACTGGGCTTTCATACATGGTCGCAACTTGA

CCAGCAGTTAATTCCAAAATCTTGAGCTGAGCTTCATGAGCAAGTCCAAAGAAAGGACCA

GCGCCTAGAATAGATGACACGGTTAAAGTCTAAATCAACGAGATCTTTGACATCTTCTGC

CTTGTCTAAAACTTTACGGGCA

ORF Predictions:

ORF # Start End Direction Length

1 1164 1640 R 159 aa

>[SEQ ID NO:155] 3864510-3 ORF translation from 1164-1640, direction R

91

SUBSTTTUTE SHEET (RULE 26) VSSILGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSLINDNTWLVFGTTT DYTRKYDLDLVREVAGDQIARRWLLSDQAFGLENVKEVALGCGGVLNDIYRVFPYIVYA QLFALLTSLKVENKPDTPSPTGTVNRWQGVIIHEYQK*

Description:

AGAS PROTEIN. - ESCHERICHIA COLI. (Probable tagatose-6-phosphate ketose/aldose isomerase)

Assembly ID: 3864526 Assembly Length: 194Obp

>[SEQ ID NO: 60] 3864526 Strep Assembly — Assembly id#3864526

TGCAGGATTTGATTTGGACGACTTTTATTATTACCAGATTCGCCTAGGAATAGAAAAAAG

AGCCCAAGAGTTGGACTATGATATCTTGCGCTATTTTAATGACCACCCTTTTACCCTAAG

CGAGGAAGTGATTGGGATTCTCTGCATCGGAAAGTTTAGTCGAGCTCAGATTTCTGCCTT

TGAAGAATACCAAAAGCCTCTTGTATTTCTAGACAGCGATACACTTTCCCTGGGACATAC

CTGTATTATCACGGATTTTTACACTGCTATGAAACAGGTTGTCGATTATTTCCTCAGTCA

AGGAATGGACCGTATCGGGATTCTAACAGGCCTTGAAGAAACAACAGACCAAGAAGAAAT

CATTCAGGACAAGCGTCTAGAAAACTTCAAAAACTACAGTCAAGCGAGGGGAATCTATCA

TGATGAACTGGTCTTTCAAGGAAGATTTACTGCCCAGTCTGGCTATGACTTAATGAAGGA

GGCCATTCAGAGCTTGGGAGACCAACTTCCGCCAGCATTTTTCGCAGCCAGCGATAGTTT

AGCTATCGGTGCCCTCCGTGCCCTCCAAGAAGCTGGAATCAGCCTGCCAGATCGCGTCAG

CCTCATTTCCTTTAACGACACTAGTCTGACCAAACAGGTCTATCCTCCCCTCTCTAGTAT

TACAGTTTATACTGAAGAAATGGGCCGAGCAGGTATGGATATTCTTAACAAGGAAGTCCT

CCACGGTCGGAAAATCCCTAGCCTGACCATGCTGGGAACCAGACTGACATTAAGAGAAAG

TACCCTAAATCAAGAATAGGATAACATAAAAAACGAATAGAGTTCTAAAACTCCTATTCG

TTTTTTATTCGATTACAATCATAGACTTAATGGTCTTACGTTCATCCATATCTTTGTAGG

CTTGGTCGATATCTTCCAGTTTATAACTTGAAGTAAAGACGCGACCTGGATTGATATCAC

CATCAAGGACGGCTTTTAGTAAAAATTGCTTATCGTATGTTGTAGCAGAAGCTGCCCCAC

CTGCTACAGAGATATTTTGCATAAATGTCGAACCAAGAGCACGATTATTATAGTGTGGGA

CTCCTACAAAGCCCATACGCCCTCCATTATGAAGAACACCTAGCGCCTGTTCTATAGCAG

CCTCCGTACCAACACATTCAAGTGCTGCGTCTGCTCCTCCGCCGAGGATTTCACGCACCT

TGGTAATTCCTTCTTGACCACGTTCTGCAACAACAGCTGTCGCACCTGACTCCATAGCCA

TCTTTTGACGGTCTTCATGACGGCTCATAAGGATAATTTGTGATGCTCCACGCATCTTAG

CCGCGATGACAGCACATTGACCAACAGCCCCATCACCGATAACAACAACCTTGTCCCCTT

TTTGAACATTTGCAACACGCGCCGCATGATAGCCTGTCGGCATGACATCTGCAAGAGTCA

AAAGGGACTTGAGCATCCCTTCTGTATAGTCAGAAGGTTGACCAGGGATTTTAACCAGCG

CCCAGTTTGCATAGTGGAAGCGAATATATTCTGCCTGAAAATCACCCCCCAAATTATTGC

CAATATGATTGTCGCAAGAACCGTCAAATCCAGCAAGACAGGCATCACACTCACCACATC

CATGTGTAAAAGGGACAATCACAAAATCACCTGGTTTCACCGTCGTAATGGCTTCCCCAG

CTTCTTCAACAATCCCAATCGCTTCGTGTCCACTTATTTTTTGTGTCCAACTTTCGTTTT

CCNTGGATTACGGTACCTCCATAAATTTGAACCACAAACGCACGCACGAACCACACGAAT

AATCACATCATCCGCTTCTATTATTTGCGGACGTTCAATGCTAGCAAGTCCAACCTGACC

92

SUBSTTTUTE SHEET (RULE 26) TGCCTTTGTATATACTGCTGATTTCATTTAAAATTTTCCTTCCTTATAAAGTTTAATTTT GAGATTTAAACGATTTAAAG

ORF Predictions:

ORF # Start End Direction Length

1 845 1660 R 272 aa

>[SEQ ID NO:156] 3864526-2 ORF translation from 845-1660, direction R

VKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYIRFHYANWALVKIPG

QPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVWIGDGAVGQCAVIAAKMRGAS

QIILMSRHEDRQKMAMESGATAWAERGQEGI KVREILGGGADAALECVGTEAAIEQAL

GVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGR

VFTSSYKLEDIDQAYKDMDERKTIKSMIVIE*

Description: ALCOHOL DEHYDROGENASE (EC 1.1.1.1). - ALCALIGENES EUTROPHUS .

Assembly ID: 3864548 Assembly Length: 2051bp

>[SEQ ID NO: 61] 3864548 Strep Assembly -- Assembly id#3864548

ATCGAATTTTTCTAGCCAGGCTACAGTTTTGGCAAGTAAGGTTTCATCTCAGGCAGTCAA

CTGGGTGAGTGCCTTTATTAGCGGAGCTTCTCAAGTGATTGTTGCCTTGATTATCGTTCC

TTTCATGCTCTTTTATCTCTTGCGTGATGGGAAAGGCTTGCGTAACTATTTGACCCAATT

CATTCCAAGAAAATTGAAGGAACCTGTTGGACAAGTTCTATCAGATGTGAATCAACAGTT

GTCCAACTATGTTCGAGGGCAAGTGACAGTGGCTATTATTGTAGCAGTAATGTTTATCAT

CTTCTTCAAGATTATTGGTCTACGCTATGCGGTTACGCTGGGGGTTACTGCTGGTATTTT

AAATCTGGTCCCTTATCTTGGTAGCTTTCTAGCCATGCTTCCTGCCCTAGTATTGGGTTT

GATTGCTGGTCCAGTCATGCTTTTGAAAGTAGTGATTGTCTTTATTGTAGAACAAACTAT

TGAAGGCCGTTTTGTCTCTCCATTGATTTTGGGAAGTCAATTAAACATCCACCCTATTAA

TGTTCTCTTTGTTTTGTTAACTTCAGGATCTATGTTTGGTATCTGGGGAGTTTTACTTGG

TATTCCGGTTTATGCCTCTGCTAAGGTTGTCATTTCAGCCATTTTCGAATGGTATAAGGT

AGTCAGTGGTCTATATGAATTAGAGGGTGAGGAAGTCAAGAGTGAACAATAGTCAACAGA

TGTTACAGGCTTTGGAGGAGCAAGATTTAACTAAGGCTGAGCATTATTTCGCCAAAGCTT

TAGAAAATGATTCAAGTGATCTTCTGTATGAGTTGGCAACTTATCTTGAAGGGATTGGTT

TCTATCCTCAGGCCAAGGAAATTTACCTGAAAATTGTAGAAGAATTTCCAGAGGTTCATC

TTAATCTAGCTGCAATGGCTAGCGAGGATGGTCAAATAGAAAAAGCCTTTAACTATCTTG

AGGAAATCCAAGCTGACAGTGACTGGTATGTCTCGCTCTTTGGCTCTGAAGGCAGACCTA

TACCAGCTGGAAGGTTTGACAGATGTGGCACGTGAGAAATTATTGGAGGCCTTGACCTAC

TCAAAGGATTCTCTCTTGATATTGGGTTTGGCAAAGTTGGATAGTGAGTTGGAAAATTAC

CAAGCGGCTATTCAAGCCTATGCCCAGTTAGATAATCGCTCGATTTATGAGCAAACGGGC

ATTTCCACCTATCAACGAATTGGCTTTGCCTATGCTCAGTTAGGGAAATTTGAAACGGCT

93

SUBSTTTUTE SHEET (RULE 26) ACTGAGTTTTTAGAAAAAGCCCTGGAGTTAGAATACGATGACTTAACAGCTTTTGAGTTG GCCAGTCTTTATTTTGATCAAGAAGAATATCAAAAAGCCACCCTCTACTTTAAGCAGCTT GATACCATTTCTCCTGACTTTGAAGGCTATGAGTATGGGTACAGTCAGGCTTTACATAAG GAACATCAAGTTCAAGAAGCCCTGCGTATCGCTAAGCAAGGATTAGAGAAAAATCCCTTT GAAACTCGCCTCTTGCTAGCTGCTTCACAATTTTCTTATGAATTGCATGATGCTAGTGGT GCAGAAAATTATCTCCTTACTGCAAAAGAAGACGCTGAGGATACAGAAGAAATCTTGCTT CGTTTAGCCACTATTTATCTGGAGCAGGAGCGTTATGAGGATATTCTAGACTTGCAGAGT GAGGAGCCAGAAAATCTTTTGACCAAGTGGATGATTGCTCGTTCTTATCAAGAAATGGAC GATTTGGATACTGCTTATGAGCATTATCAAGAGTTGACAGGAGATTTGAAGGACAATCCA GAATTTCTGGAACACTATATCTATCTCTTGCGTGAATTGGGACATTTTGAAGAAGCAAAA GTCCATGCTCACACTTACTTAAAACTGGTTCCAGATGATGTGCAAATGCAAGAACTGTTT GAGAGATTGTAAGAATGTTTAAACATATAGAACTGTAGTTTATCTCTTTTGATAGCTACG GTCTTTATTTGTACATGGTAGAATCTTTTTACAAAAATACTTGGTAATCTTGTTTATTCA TGCCATAATAG

ORF Predictions :

ORF # Start End Direction Length

1 687 1055 F 123 aa

2 979 1932 F 318 aa

>[SEQ ID NO:157] 3864548-2 ORF translation from 687-1055, direction F

VRKSRVNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEGIGFYPQAKEIY

LKIVEEFPEVHLNLAAMASEDGQIEKAFNYLEEIQADSDWYVSLFGSEGRPIPAGRFDRC

GT*

Description: unknown

>[SEQ ID NO: 158] 3864548-3 ORF translation from 979-1932, direction F

VTGMSRSLALKADLYQLEGLTDVAREKLLEALTYSKDSLLILGLAKLDSELENYQAAIQA

YAQLDNRSIYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFD

QEEYQKATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLL

AASQFSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENL

LTKWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY

LKLVPDDVQMQELFERL*

Description: unknown

Assembly ID: 3864582 Assembly Length: 1318bp

94

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 62] 3864582 Strep Assembly — Assembly id#3864582

CTTTAGCAATCAGTTTATTGGGAGATTTGACTGCCACTTCTGTTGGAACCTTGATAATCT

TTTTACCCTCAAAGCGTTCCATACCAGAAATCTTAACATCAACTGCTAAAATAACTACAT

CCGCTGCATCAATCTGCTCTTGACTCAATTCATTTTCTACCCCTATTGTCCCCTGAGTCT

CAACATGAATCACATGTCCAGCTACCTTTGCGGCATTCTCTAATTTTTCCTGTGCAATAT

AAGTGTGGGCAATTCCCATAGTACAAGCTGCAACACCAACAATTTTCATACGGATACCCT

CCAAAATTTTTTCTTATTAACAAAAAGCTGCAATCACATCATCAGATGTCTGAGCCCGAA

CTAATTTGGCAACAACTTCGTCATTACCAAGTTTTCGAGCAAAGAGTGATAAGGTCTTCA

AATGCTCCCTAGCAGCTTCTGTATCATCACCAACTGCAAAGAGTACAATTACTTTGACCC

CTTTCCCATCAATGGTCTCCCAAGGAATCTCATTGTGATTTATAGCTATGACTACCCCCG

CCTTCTCCACAGCAGAACTCTAGCTATGGGGAATAGCAATATAATTCCCAATACCGGTCT

GTCCTTCTGCCTCTCTCTGATAAAGACCTTCGATAAATTGGTCTCTATCAGACACATAAC

CCGTCTCAACCAATAGTATGAGCTAATGCCTCAAAAACCTCTTCTTTGCTCTGCATCTGT

AAATCCGTCTGGATCAGACTCACATTAAGAATATCTTTGATTTCCATATATTATCTCCCG

TAATTCTTCTTTTGTTAACTGTTTTAATTGATTTATGAATGATTCATCTGCTAGTCTTCT

CATCAATGTTTTAATACATGACTTGTCCTGTGATACTGCAATGGCCAAACCGATAATAAG

GTCAACACACTGGATATCCTTCGACCATTCTCTGATAGGTGGTTTTAATCTAGTAATCAC

TAAGACATGATGTTGAAAGTTTCCTTCACAATGTGGTAGAAGAACACCTTTAGCAACCTC

TATACTTCCCTGTCTCTCACGGTAATATAGAAGCTCTTCTATTTTTTCTGTATCTTCAGA

AACAAGAAGGCTGATTTGATTTGCTAATTCTTTGTAGGCTTCTTGACGATTTTGAACAGA

TATATCCATAAGGACAAGCGAAAGATTATTCATAGTTTATCTCCTGAATTTTTGCTTGAA

GACGTTGTTTATCACCCTCGGTTAGAAAAGCACTAACTAGGACAAACGGGACACTTGCTG

GTTCCTGCAAAGCTACCGTCGTCACAATGAAATCTAAATCTGGATATAGATTTATCAG

ORF Predictions:

ORF # Start End Direction Length

1 317 550 R 78 aa

>[SEQ ID NO: 159] 3864582-1 ORF translation from 317-550, direction R VEKAGWIAINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLSLFARKLGNDEW AKLVRAQTSDDVIAAFC*

Description: Probable phosphotransferase enzyme Ila component

Assembly ID: 3864604 Assembly Length: 2077bp

>[SEQ ID NO: 63] 3864604 Strep Assembly -- Assembly id#3864604 CTAGTCTTGGCTACTGTCTAAGTTGGCTTGTGCATAAGCCTGCCAGATTTTTTGTTGGGG TTTGGCAAGTGGGTAATTCTTGAATTCTTCTGGTGAAAGCCAACGAACTTCCCTATCTGA AAAATCATGGAAGTCACTCACCTGACCTGCTACAATCTGTACATGCCATTTTCGATGACT

95

SUBSTTTUTE SHEET (RULE 26) AAAAACATGCTGGACTGTATCAAAACAAACATCAAGCCAATCAACATCTAGGTCATAGTC CTGCTGGAAACTCTCTTCTGGGACTGGGGCCAGAGTTCACACTTTCTTCCGCAACCTGAT GAAAGAGGTCAAACTGCTCTTCTTGCGAAAAGTTATCAACTTCTATAAAGGGGAAATGCC AAAAACCTGCCAAGAGCTTTTCGCTTTCATTTTTTTCAAGTAAAAATTGTCCTTGAGAAT TTTTCACAACTAAGGCTTTAAGATAAATAGGAACCGGCTTTTTCTTAGGAGATTTAATTG GATAACGGTCCATGGTTCCATTCTGATATGCCGCACTAAAGTCCTTGACTGGGCTTTCTT CAGGTCTGGGATTTACAGGAGACTCAATATCAGACCCTAAGTCCATCAAGGCTTGATTAA AATCACCCGGACGATCTGGATTAATCAAGATCTCCATCATTGCCTGAAAAATTTTTCGAT TACTTGGAATCCCAATATCGTGGTTGACTTCAAACAGACGCGCCAAGACCCGCATGACAT TACCATCTACAGCTGGCTCAGGCAAGTTAAAAGCAATACTGGAAATGGCTCCTGCTGTGT AAGGTCCAATCCCTTTCAAGCTGGAAATTCCTTCATAGGTATTTGGAAATTGGCCACCAA AGTCAGTCATAATCTGCTGGGCTGCAGCCTGCATATTGCGAACTCGAGAATAATAACCCA AGCCCTCCCAAGCTTTCAGTAAACTCTCCTCAGGCGCAGTTGCCAGACTTTCGACAGTTG GAAACCAGTCCAAAAATCTTTCGTAGTAAGGGATAACTGTATCCACCCTGGTCTGCTGAA GCATGATTTCAGATACCCAGATGTGATAAGGATTTTTACTTCTCCTCCAAGGCAAATCTC TTTTGTTTTCATCATACCAAGCGAGAAGTTTTCTCACCGGAAAGAAATGACTTTCTCCTC CGGCCACATGACGATACCGTATTCTTTCAAATCCTAACATATCTCTAGTTATAACACAGA AGGTTTCACCTGTCTTTGTATCTGATTTATAATATTTTCAATAGATAGTATATAACTTTT CCTATCTACTTATACTCCAATGAAAATCCAAAGAGCAAACTAAGAAGCTAGCCGCAGGTT GCTCAAAACACTGTTTTGAGGTTGTGGATAGAACTGACAGAGTCAGTATCATATTACCTA CGGCAAGGTGAAGCTGACGTAGTTTGAAAAGATTTTCGAAGAGTATAAATCTTATTGATG AACTGCTTGCAGTCTGAGAAAAAATGAGCTTGGATATTATTTCCAAACTCACTTAAAGTC AATTTCAATCCACTAGAACAAGCCTAGTACAGTTCCATCGCTTTCAACATCCATGTTGAG AGCTGCTGGACGTTTTGGAAGACCTGGCATGGTCATAACATCACCAGTTAAGGCAACGAT GAAGCCTGCACCTAATTTTGGTACCAATTCACGAATGGTAATTTCAAAGTTTTCTGGTGC TCCAAGCGCATTTGGATTGTCTGAGAAACTGTATTGAGTTTTAGCCATACAAATTGGCAA TTTGTCCCAACCGTTTTGAACGATTTGAGCAATTTGTGTTTGAGCTTTCTTCTCAAAGTT CACTTTGCTACCACGATAGATTTCAGTGACAATTTTTTCAATCTTTTCTTGGACAGAAAG GTCATTATCGTACAAACGTTTATAGTTAGCTGGATTTTCAGCAATTGTCTTAACAACTGT TTCGGCAAGTGCTACTCCACCTTCTGCTCCATCAGCCCAGACACTAGCCAATTCAACTGG TACATCGATTGAGGCACAGAGTTCTTTTAAGGCTGCAATTTCAGCTTCTGTATCAGATAC AAATTCGTTAATAGATACAAGCTAATGGAATACCGAA

ORF Predictions:

ORF # Start End Direction Length

1 1 141 R 47 aa

2 1513 1803 R 97 aa

>[SEQ ID NO:160] 3864604-1 ORF translation from 1-141, direction R VSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD*

Description: unknown

>[SEQ ID NO: 161] 3864604-3 ORF translation from 1513-1803, direction R

VNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEITIRELVPKLGAG

FIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF*

Description:

FORMATE--TETRAHYDROFOLATE LIGASE (EC 6.3.4.3) (FORMYLTETRAHYDROFOLATE SYNTHETAS E) (FHS) (FTHFS). - CLOSTRIDIUM ACIDI-URICI .

Assembly ID: 3864610 Assembly Length: 1887bp

>[SEQ ID NO: 64] 3864610 Strep Assembly -- Assembly id#3864610

CTCAAAACNCTGCTTTGAAGAGATTTTCAAAGAGTACAAGAAGTTTAGTTATTAGCGTTC

TTACCGCTTGTAAACTAGATTTCTCATAAAATAGAATCTTTTCCTTTTAGTTGTAAACTA

GTCTGGGAGAGTAGAGAGGTTTGAGATACCTTTCTAGCTTTTGGATTATCATCTAAGAAG

AGTAATTTCCCTTGCATTAAAAAGGGGAAAAAGAGACACGAAATGACTATAATGGGTGAC

AATGGGGGAAGGGATAGACAAGAGATTTTATCCACATATGAAAAAAGGAGGTTAGGAAAG

AGTTATATATCCTATATTATATAAATAATCAATTGCGCAGAAATTTGGTAAGAATTCATG

CGTCAACTCATAAAGAACTACTTAAAAAATTCACAGTATTCATAATTATTTTCGAGGAGA

AAAACAGTGAAAAAAAGAAAAAAGCTTGCTCTGTCTCTTATCGCTTTTTGGCTGACGGCT

TGTTTAGTAGGCTGTGCTAGCTGGATTGATCGTGGAGAATCCATAACGGCTGTTGGCTCA

ACTGCCTTGCAACCCTTGGTTGAAGTAGCGGCAGATGAATTTGGCACCATCCATGTTGGA

AAAACGGTCAATGTCCAAGGGGGAAGTTCTGGTACAGGCTTGTCCCAGGTTCAGTCTGGG

GCAGTTGATATAGGAAACTCAGATGTATTTGCTGAGGAAAAAGACGGAATTGATGCTTCT

GCTCTTGTTGACCACAAGGTCGCGGTAGCTGGCTTGGCTCTGATTGTCAATAAGGAGGTT

GATGTTGATAACCTAACGACAGAGCAACTTCGTCAAATCTTCATAGGTGAGGTAACCAAT

TGGAAAGAGGTTGGTGGTAAGGACTTACCCATCTCTGTTATCAATCGGGCAGCCGGCTCT

GGCTCTCGTGCTACCTTTGATACTGTCATTATGGAAGGTCAGTCTGCCATGCAAAGTCAG

GAGCAGGATTCAAATGGAGCGGTAAAATCAATCGTATCAAAAAGTCCAGGAGCTATCTCT

TATTTATCTCTTACCTATATAGATGATTCGGTCAAAAGCATGAAGTTGAATGGCTATGAC

TTAAGTCCAGAAAATATAAGTAGCAATAATTGGCCCTTGTGGTCTTATGAGCATATGTAT

ACATTGGGGCAGCCCAATGAGTTGGCTGCAGAATTTCTCAATTTTGTTCTCTCGGATGAG

ACCCAAGAAGGGATTGTCAAAGGATTGAAGTATATTCCGATTAAGGAAATGAAGGTTGAA

AAAGATGCTGCCGGAACTGTGACAGTGTTGGAAGGGAGACAATAATGAATCAAGAAGAAT

TAGCTAAGAAAATGTTGCTTCCATCAAAGAATTCTCGTCTGGAGAAATTAGGAAAAGGTT

TGACCTTTGCCTGTCTTTCTTTGATAGTCATCCTTGTGGCCATGATTTTGGTTTTCGTAG

CGCAAAAAGGCTTGTCGACCTTCTTTGTCAATGGTGTGAATATCTTTGACTTTCTTTTGG

GAGGAACTTGGAATCCTTCTAGTAAAGAATTTGGTGCCCTTCCTATGATTTTGGGTTCCT

TTATCGTTACCATTCTCTCAGCCCTTATCGCAACACCCTTTGCTATTGGTGCAGCAGTTT

TTATGACCGAAGTATCACCAAAAGGGGCGAAGATTTTGCAACCAGCTATTGAACTCCTGG

TTGGGATTCCTTCAGTAGTGTACGGATTTATTGGCTTGCAAGTCGTCGTTCCCTTTGTTC

97

SUBSTTTUTE SHEET (RULE 26) GCAGTGTCTTTGGTGGGACTGGTTTTGGGATTTTGTCAGGGATTTCCGTCCTCTTTGTCA TGATTTTGCCGACCGTAACCTTTATGACAACGGATAGCTTGCGTGCGGTTCCTCCNTTAT TATCGTGAAGCCAGTTTCGCTATGGGA

ORF Predictions :

ORF # Start End Direction Length

1 427 1305 F 293 aa

>[SEQ ID NO:162] 3864610-1 ORF translation from 427-1305, direction F

VKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPLVEVAADEFGTIHVGKT

VNVQGGSSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVDHKVAVAGLALIVNKEVDV

DNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGSRATFDTVIMEGQSAMQSQEQ

DSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYDLSPENISSNNWPLWSYEHMYTL

GQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMKVEKDAAGTVTVLEGRQ*

Description:

PROBABLE ABC TRANSPORTER BINDING PROTEIN PRECURSOR (ORF108) . - BACILLUS SUBTILIS. (BLAST)

Assembly ID: 3864716 Assembly Length: 405bp

>[SEQ ID NO: 65] 3864716 Strep Assembly -- Assembly id#3864716

CTGAGGAATCAAAAGTTGAACCACCAGTAGAACAAGCATAAGTCCCAGAACAACCCGTGC

AACCTACACAAGCTGAGCAACCAAGTACACCAAAAGAATCATCACAACAAGAAAATCCTA

AAGAAGATAGGGGAGCGGAAGAGACTCCGAAACAAGAAGATGAACAGCCAGCAGAAGCCC

AAGAAATCAAGGTTGAAGAACCAGTAGAATCTATAGAGGAGACTGTCATTCAACCTGTTG

AACAACCAAAAGTGGAAACGCCTGCTGTTTAATAACTAACGGAACCTACAGAGGAACCTA

AAGTTGAAGTAACTAGTATTCCCCTCACTACTCGCTATGAGGAAGACCTTACTTACGAAC

ACGGAACGCGTTGAAGTTGTTAAGGAAGGTTATAATTGGCAGTAT

ORF Predictions:

ORF # Start End Direction Length

1 57 272 F 72 aa

>[SEQ ID NO: 163] 3864716-1 ORF translation from 57-272, direction F

VQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAQEIKVEEPVESIEETVIQP

VEQPKVETPAV*

Description: unknown

98

SUBSmUTE SHEET (RULE 26) Assembly ID: 3864718 Assembly Length: 1542bp

>[SEQ ID NO: 66] 3864718 Strep Assembly -- Assembly id#3864718

CTATGGGATTGGTAGTTCTTCCTAGTGCAGGGGCTGTAGACCCAGTTGCGACCCTAGCGC

TGGACTAGTCGAGAGGGTGTTGTTGAAAATGGATGGCTATCGCTATGTTGGTTATCTATC

AGGTGACATCCTCAAAACGCTTGGCTTGGACACTGTTTTAGAAGAAACCTCAGCAAAACC

TGGAGAGGTGACTGTAGTCGAAGTTGAGACTCCTCAATCAACAACAAATCAGGAGCAAGC

TAGGACAGAAAACCAAGTAGTAGAGACAGAGGAAGCTCCAAAAGAAGAAGCACCTAAAAC

AGAAGAAAGTCCAAAGGAAGAACCAAAATCGGAGGTAAAACCTACTGACGACACCCTTCC

TAAAGTAGAAGAGGGGAAAGAAGATTCAGCAGAACCATCTCCAGTTGAAGAAGTAGGTGG

AGAAGTTGAGTCAAAACCAGAGGAAAAAGTAGCAGTTAAGCCAGAAAGTCAACCATCAGA

CAAACCAGCTGAGGAATCAAAAGTTGAACCACCAGTAGAACAAGCAAAAGTCCCAGAACA

ACCCGTGCAACCTACACAAGCTGAGCAACCAAGTACACCAAAAGAATCATCACAACAAGA

AAATCCTAAAGAAGATAGGGGAGCGGAAGAGACACCGAAACAAGAAGATGAACAGCCAGC

AGAAGCCCAAGAAATCAAGGTTGAAGAACCAGTAGAATCAAAAGAGGAGACTGTTAATCA

ACCTGTTGAACAACCAAAAGTGGAAACGCCTGCTGTAGAAAAACAAACGGAACCAACAGA

GGAACCAAAAGTTGAAGTAACAAGTATTCCCCAAACTACTCGCTATGAGGAAGACCTTAC

TAAGGAACACGGAACGCGTGAAGTTGTTAAGGAAGGTAAGAATGGCAGTAGAACAGTTAC

TACTCCATATATCTTGAATGCGACAGATGGTACGACTACAGAAGGCACTTCGACAACTGA

TGAAGCTGAGATGGAGAAAGAGGTTGTTCGTGTTGGCACGAAACCCAAAGAAAAATTAGC

TCCAGTCTTAAGTTTGACAAGTGTTACAGATAATGCAATGTTGCGTAGTGCGAGACTTAC

TTATCATTTGGAAAATACAGATAGTGTTGATGTGAAAAAAATTCATGCTGAAATTAAAAA

TGGCGATAAGGTTGTCAAAACTATTGACTTATCTAAAGAGAGATTATCAGATGCTGTTGA

CGGTCTTGAACTTTATAAAGATTATAAGATTGTGACGAGTATGACCTATGATAGAGGTAA

TGGTGAAGAAACCTCTACGTTGGAAGAAACTCCACTACGATTAGACCTCAAGAAGGTTGA

ATTGAAAAACATCGGCTCTACTAATCTCGTCAAAGTAAATGAGGATGGTACTGAGGTGGC

AAGTGACTTCTTAACAAGTAAACCTGTGGATGTGCAGAATTACTACCTCAAAGTAACTTC

CCGTGATAATAAAGTTGTTTCCCCTCCCAGTTGAAAAAATTGAAGAGGTGACTGAGGAAG

GTCCACCACTTTACAAAGTCCCTGCTAAGGCCCTAATTTGAT

ORF Predictions:

ORF # Start End Direction Length

1 77 1474 F 466 aa

> [ SEQ ID NO : 164 ] 3864718 -1 ORF translation from 77 -1474 , direction F

VLLKMDGYRYVGYLSGDILKTLGLDTVLEETSAKPGEVTWEVETPQSTTNQEQARTENQ

WETEEAPKEEAPKTEESPKEEPKSEVKPTDDTLPKVEEGKEDSAEPSPVEEVGGEVESK

PEEKVAVKPESQPSDKPAEESKVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKED

RGAEETPKQEDEQPAEAQEIKVEEPVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVE

VTSIPQTTRYEEDLTKEHGTREWKEGKNGSRTVTTPYILNATDGTTTEGTSTTDEAEME

99

SUBSTTTUTE SHEET (RULE 26) KEWRVGTKPKEKLAPVLSLTSVTDNAMLRSARLTYHLENTDSVDVKKIHAEIKNGDKW KTIDLSKERLSDAVDGLELYKDYKIVTSMTYDRGNGEETSTLEETPLRLDLKKVELKNIG STNLVKVNEDGTEVASDFLTSKPVDVQNYYLKVTSRDNKWSPPS*

Description: unknown

Assembly ID: 3864802 Assembly Length: 1321bp

>[SEQ ID NO: 67] 3864802 Strep Assembly -- Assembly id#3864802

ATCGAATTACTTCAACTCCAACTTTACTCTCAATAAAAATCAAATGTAAAAAGAGGAGCT

AAATTTATCTTTTTCTCCTCCTTCATCGTTCTTACTTTTGACCATAATAAGCATTTGGTC

CATGTTTACGTTGGTAGTGTTTTTCTAGTATGTACTGGGGAGCAGGTTCAACTCTTGGAT

TGATTTGTTCTGTAAAGCGATTCATCTTTGATACTTCCTCTAGTACGACAGAGTGATAAA

CAGCATTCTCTGGATTTTTGCCCCAGGTGAATGGACCGTGATTGCGTACAACAATTCCTG

GTACTTCAACCGGGTTAAGTCCGCGATGTTCAAACTCTTCTACGATAACCAGGCCAGTAT

CTTTTTCATAGGCCACTTCTACTTCGTCCTTGGTCAAACTACGGGCGCAAGGGATTGAAC

CGTAGAAATAATCTGCATGGGTTGTTCCGTAGAAAGGAATATCACGACCTGCCTGAGCCC

AAGCAACAGCTTCTGTCGAATGGGTGTGAACCACACTACCAATTTCTGACCAAGCCTTAT

ATAATTGCACATGAGTTGGGAAGTCGGAAGATGGTCTTAAATCCCCTTATAGGATCTTAC

CATCTAGATCAGTCACTACCATGTTTTCAGGTGTCAATTCGTCATAATCCACGCCTGATG

GTTTGATAACAATGACACCGAGTTCGCGATTGACTTCAGATACATTCCCCCAGGTAAATT

TGACAAGTCCATGTTTTGGCAATGATTGATTGGCATCACAGACTCGTTTACGCATAGCAT

TGATTACTTGATTCATCTTACATCAAACCTGCTTTCTTAATGAGTGGATAGAGAAAAGCT

TGCGCCTCTTGAATGGCTGCGCGTGTTTCTTCTACTGTTTCACAATTTTCAGACCACATT

TCGATTAGGAAAGGTCCATTATAATTGGTTTCCTTTAAAATATCGAAAGCTTCTTCCCAT

TTGACACAACCTTGCCCAAAAGGTACATCTCGGAACTGGCCCTTTGAACTTTCTGTCACT

GCATAAGTATCCTTGAGATGGAGAGTTGCGATGGCATGATGACCAAGATAAAACTCACTA

TAGATATCATTATGCCATGCAGACACATTACCAATATCTGGATATACAAAGAGGAAGGGA

GAGTCAATCTCTTTTTCTATAGCCAAATATTTTTCGATGCTATTGATGAAAGGATCATCC

ATAATTTCAATAGCAAGTACCACCTGAGCTTCTTCAGCCCAGTCACAGGCTTTTCTCAAA TTTTTGATAAAACGTTGGCGTGTCTGGGGTGACTTTTCCTCATAGTAAACATCGTAACCA

G

ORF Predictions:

ORF # Start End Direction Length

1 92 550 R 153 aa

>[SEQ ID NO:165] 3864802-1 ORF translation from 92-550, direction R VQLYKAWSEIGSWHTHSTEAVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVA YEKDTGLVIVEEFEHRGLNPVEVPGIWRNHGPFTWGKNPENAVYHSWLEEVSKMNRFT

100

SUBSmUTE SHEET (RULE 26) EQINPRVEPAPQYILEKHYQRKHGPNAYYGQK*

Description: L-RIBUL0SE-5-PH0SPHATE 4-EPIMERASE (EC 5.1.3.4). - ESCHERICHIA COLI.

Assembly ID: 3864854 Assembly Length: 1265bp

>[SEQ ID NO: 68] 3864854 Strep Assembly -- Assembly id#3864854

TTTTTCTGTTTTTCGGAGCAAACTGGGCTCCAGCCGGTTTTGGCCTTCTTTCCTTAGCTA

CAGCTGGTTTAGCTGGCTCAGATTTTTCGGCTTTCTTTTCTGCACTTACTTTTGGTGCTG

CAGGTTTTGCTTCTACTTTCGGAGCAGCTGCAGGCTTAAAGCTGGCAGCAATTTTTGCAG

CGACAGCTTCTTCCACACTTGATGAGTGGCTTTTCACATCCAAGCCCAACTCTTTTGCAC

GCGCTACAACTTCTTTACTTTCTTTTCCAAGTTCTTTTGCGATTTCGTACAATCTTTTCT

TAGACAAATCATGTCCTCCTCTTCTATTCCATAAGAGACCTCATTTTCTTTGTAAATCCA

GCATCTGTTACAGCCAAAACCTTTCTCGATTTCCCGACTGCTATGATTAATTCCAGTGTT

GAAAACACGGTTACAATTTCTACTTGATAATAATGACTTTTATCTTGAATCTTCTTGGTC

AGATTGGGTCCAGCATCATGAGCTAGAAAGACCAACTTGGCCTTGCCGTCTTGAATGGCC

TTGACCACCAATTCTTCACCCGATATGATGCGCCCTGCTCGCTGAGCAAGCCCCAAGAGA

TTACTTATCTTTTGCTTATTCAAGTCCCAACTCTCTTCTTTTCACTTTGTGATCCACATA

AGCGATCAACTCGTCATAAAAGCTTTCTTCCACTTCCATGCTAAAGCTGCGGTTAAAGAC

CTTCTTCTTTTTCGCCTCTAGGGCTTCTGCATTGTCTAGTTTGATATAAGCGCCGCGGCC

ATTGGCCTTGCCCGTAGGATCAATAAAGACTTGTCCTTCCTTGTTCTTGACAATGCGGAG

CAAATCACGCTTATCAATCACTTCGTTAGACACAACAGACTTGCGCAAAGGGATTTTTCT

TGTTTTCATCTTTCCCTCCTCTAGCAGCTTTTATTCTTCTACAGTATCGTTTTCTACTTC

CAACTCTACTGAAGCAGCGTCTTCCATGGCTTCAAATTCGCTAGCAGACTTGATATCGAT

ACGGTAACCAGTCAAGTGAGCCGCCAAGCGCACGTTTTGTCCACGACGACCAATGGCAAG

AGAAAGCTTGTTATCTGGAACAACCACCAAGGCACGTTTGCTGTCGTTTTCATCAAAGAT

AACTTGGTCAACCTCAGCAGGAGCGATGGCATTGTAGATAAATTCAGCTGGATCTGCTAC

CCACTCGATAACATCGATATTTTCTTCGATTGGTACCATGCGGTCATTTTTAGCATCGTA

ACGAG

ORF Predictions:

ORF # Start End Direction Length

1 324 548 R 75 aa

>[SEQ ID NO:166] 3864854-1 ORF translation from 324-548, direction R WKAIQDGKAKLVFLAHDAGPNLTKKIQDKSHYYQVEIVTVFSTLELIIAVGKSRKVLAV TDAGFTKKMRSLME*

Description:

101

SUBSTTTUTE SHEET (RULE 26) PROBABLE 11.1 KD RIBOSOMAL PROTEIN IN NUSA-INFB INTERGENIC REGION (ORF4) . - BACILLUS SUBTILIS.

Assembly ID: 3864862 Assembly Length: 1305bp

>[SEQ ID NO: 69] 3864862 Strep Assembly -- Assembly id#3864862

ATAAACCAAAGGAAGCTGAGCTCTTTAGTCCCAGCTTCTTTTTATATATAAAATTTTACC

CGTGAAAAGACAGGGCCTTAGCAGACTTCTTTTTTACTTCGTTCACCCTTGCTTTTTCTT

TGTATGTTTGGGCGTTGGCAGTTGGTTATACATAGCTAAAATCAGGTCTTATAGAAACAT

CTTATTATCAAGTTCTTCCACTCAAATCATTTCTTTGGCACCTTTGTATGGAAACTCAAA

AGAAGATTGGTCAATCTTATCTAAGACTGCTTGCACGGGTTTAACTAAAAGCGATCGTCA

TAAATGCCGCCAATAATCTTGCCGCGGAAGTAAAGAATATACTCCCCCATCATGGAACGG

TAAGTCACATCATCTAATCCTGATAATTGTTCCAAAACAAATTCCAAATAGTTCTTACTT

GATGCCATTTCTAATCTTCTAGGCTCTGTTCAACGATAACAACCGTATAGAGTTCTTGCT

TAACCTCGCATCCAATTGATTTAAAGCCCTGCTTTTCCCAAAAATGCTGAGATTGCGGAT

TTCCCTTAACATAAGCCAAACGTGCCTTTCGAAAGTTCTTAGCAAAATAAGCTAGTGCTT

CTGTCACAATATGACTACCAATCCCTTTCCTCTGATAGGCTTGATCAACCATAAACAAAC

CAATAAAAACAGTCTCCTCATCAGGATATGCATAGACAAAATCCATAACAGCCACAAGGT

CAAATCCATTCCAAAATCCAACAAAAAACTTATCAGCCTTAGCTTTACCTTCAGGTAGAC

AAAGCATGTCCTCTTTTACAGTTGCAAAATTTGGCTCTGGTGGACAATGCTGAAAATACA

GAGGATTACTTTCATATAAAGATAAAATACTTGGAATATCCTTTTCAGTTAGTATCCTAC

AACTGTAATACTTAGATAGTTGGTCAATCATCTTTTCAAATTCGATACTTTCTTGTGCCC

TGTGATTATGACACAGGAAGATGCACTGATCGTCATCAGCCACATAAAAGTTCTTTCCAT

CGTGCCTAATCGTTGTCTCAAACCTTTGGATAAAACCTTTAGCCTATACAACTGGATTTT

CCTCTCTCAAAAGTATATTCTTTTGCAGGCGAACTTCCTCAAAATCAGTCGTGTGCAACT

TCAGTAGAATATTCATAGGCTCGGATAATCTGAGCGACAACAGGATGGCGAACCACATCC

TTGGCTGAAAAATGAACAAAGTCAATCTGATGGATGTTCTTGAGTTTCTCTTGAGCATCA

ATCAAACCGGACTTGACATTACGTGGCAGGTCAATCTGACTAATA

ORF Predictions:

ORF # Start End Direction Length

1 431 1003 R 191 aa

>[SEQ ID NO: 167] 3864862-1 ORF translation from 431-1003, direction R

VADDDQCIFLCHNHRAQESIEFEKMIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHC

PPEPNFATVKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVD

QAYQRKGIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYT

WIVEQSLED*

Description : unknown

102

SUBSTTTUTE SHEET (RULE 26?> Assembly ID: 3864888 Assembly Length: 1742bp

>[SEQ ID NO:70] 3864888 Strep Assembly — Assembly id#3864888

CTAATCTCCTTAAAACGTGATCTTTTCAAGAATATTTTTATCTAAACAATCCAGCAAGTC

TTGGTAAGAATAGACTTCGTAAGTCGGCTGGGCTTGTGTGTGATTTTCGAGGTGATGAGG

ATTATACCAGATAGTGTCAATCCCCGCATTATTGCCACCTTGAATGTCGGCGGTTAGAGA

ATCTCCAATCATCAGCGTCTTTTCTTTACTAAATCCAGCAATTTGCTGGCCAATCTTTTC

ATAAAAAAGAGCATCCGGCTTTTGAGTTTGCAACTGTTCTGAGATAAAGACTTGATTGAA

ATAAGGTGCTAGACCAGATTGAGCCAAACGTCCTGTCTGAATGGCAGTAATGCCATTTGT

CGCAGCATACAAGTTATAATCACGCTCAATGAGGCTGTCCAAGAGATCATGAGCGCCCGA

TAGTGTTTGTCCCTGCTGGGCGAGGTAAAATTGGTAACGCTGGGCAAGAAAACTACCGTC

TTTTTCCTGTCCAAAATGAGCAAATAAACGAGAAAAGCGCGTGTTAACCAGCTCTTGTTT

ACTGATTTTCTTCAGCTCCAAGTCTTTCCAGAGAGCCTTGTTCATAGGAACGTAATAATC

TTTATAAGCCGGAATATCCGCAACTCCTTCTTCTTTTAGAAGTGGAGTCAAAGCCACATC

CTCAGCAGCATCAAAATCAAGAAGAGTGTGGTCGAGGTCGAAGAGTACAAATTTGTAGAA

CAATTTGAGGTTTTCCTTTCTGAAAATTCATTAAGAACATTATATCATAAAGCACCTCAT

ACAATTAACTAATTTAATCACTTAAAAAAAATTCGAACACTTTCTATACAACTGACAGCT

CAAATCTTTCAGAATAGAACAATACTAACTATCGAACACCCCGTCTTCATAAATACATAT

GTAATTCTAGGCCTAGAATTCCTATAAACTAAATGCTTTCATACTCTTCCAAGTAATTGA

TTGCCTTAAATTTTAATTTTTGAAGGTTTCTAAAGCTAGAATAGCCCCATCACAATCAGT

TTTGATTGATTCACAATTTAGAAACACTATAGTTTCACTCCTGTTAAAATAAAAAGGAAC

TGCATAAAGCAATCCCTTTCTGATTTTGAAATCATTTACTTAACATTTTATAGTTGAGAT

AATCAATAGCTTATCTATAAAAAGAGTTATAGTAAAATTCCTTATTTATTGATTCCAAGC

TCCGCTAACTGTATTTGAATAACTGACAGTTCTGCACCAGCCTGAAAAAGAGCAGCTGCA

TTATAGGCACCTTCTACAATTGGAACCCTGTTGATGATGATACTTTTATCACTGAAATCA

GTCACCATTTTTAAGTTCATTTTAGCAGAACCTAGGTCAAAAAAGGCAAGTAAAGTATCT

GCTGGATTTTCGGAAACAACCCTATCTACTTGATCAAAACTCGTTCCAATTCCTCCGCCC

TCGGTTCCTCCTACATAAGTAATCGGAACATCTTTAGCTACTTTACTAATCAGTTCAACA

ACACCTTCTGCAATGTGTTTGGAATGTGAAACGATAACAAGACCAATACCAATACTTTCC

ATCAAACCACTCCAGTTTCTAAAATAGCAGTAAAGAGTAATCCTGATGAGAATGATCCAG

GATCAATATGTCCAAGAAACCACATGCTCCTAAGACAAGAGCTAACAGACTGGCCATCAA

TAATAGTATTGTTCTTTTTTTCATCATTACTCCTTAACTAGTGTTTAACTGATTAATTCG

AT

ORF Predictions:

ORF # Start End Direction Length

1 10 657 R 216 aa

>[SEQ ID NO: 168] 3864888-1 ORF translation from 10-657, direction R VALTPLLKEEGVADIPAYKDYYVPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKD

103

SUBSTTTUTE SHEET (RULE 26) GSFLAQRYQFYLAQQGQTLSGAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYF NQVFISEQLQTQKPDALFYEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNP HHLENHTQAQPTYEVYSYQDLLDCLDKNILEKITF*

Description: unknown

Assembly ID: 3864898 Assembly Length: 1136bp

>[SEQ ID N0:71] 3864898 Strep Assembly -- Assembly id#3864898

GTGGAATGCGGGGACGCCTTGTCTAATTTTGGATCAAGCCCTGAGTTTGACACAGGGAAA

TGAGCTGGACGGACTGCTATCTCTGAAGAAATTACTGGCACCATTAGCCTATCAGCCTTG

GATGATTATGTGGCGGCCTTGTCTCAACAGGATGTTCCCAAAGCTTTGTCTTGCTTGAAT

CTTCTTTTTGACAATGGTAAGAGCATGACTCGTTTTGTGACCGATCTTTTGCACTATTTA

AGAGACTTGTTAATTGTTCAAACAGGGGGAGAAAATACTCATCATAGTTCAGTCTTTGTA

GAAAATTTGGCACTTCCTCAAAAAAATCTGTTTGAAATGATTCGCTTAGCAACAGTGAAT

TTAGCAGATATTAAGTCTAGTTTGCAGCCCAAGATTTATGCTGAAATGATGACCGTCCGT

TTGGCGGAAATCAAGCCCGAACCAGCTCTATCAGGAGCGGTTGAAAATCGAATTGCTACG

CTGAGACAGGAAGTTGCCCGTCTCAAACAAGAGCTTTCTAATGCAGGTGCGGTTCCTAAA

CAAGTTGCACCAGCTCCTAGTCGACCAGCTACGGGCAAAACAGTCTATCGTGTCGATCGC

AATAAAGTGCAATCTATCTTACAAGAGGCCGTCGAAAATCCTGATTTAGCACGTCAAAAT

CTAATTCGTTTGCAGAATGCCTGGGGAGAGGTAATTGAAAGTCTAGGTGGGCCGGACAAG

GCTCTGCTAGTTGGTTCTCAACCGGTTGCTGCCAATGAACACCATGCTATTCTTGCTTTT

GAGTCTAACTTCAATGCTGGTCAAACTATGAAACGAGACAATCTCAATACCATGTTTGGT

AATATCCTCAGTCAGGCGGCAGGTTTTTCACCTGAGATTTTAGCTATTTCCATGGAGGAA

TGGAAAGAAGTTCGCGCAGCCTTTTCAGCCAAAGCCAAATCTTCTCAAACTGAAAAAGAA

GTAGAAGAAAGCCTGATTCCAGAAGGATTTGAATTTTTGGCTGATAAAGTGAAGGTAGAG

GAAGACTAAAGAAAGATTTCATGATACAATAAGTTTATGAATAAACAACAATTTATTATT

ATGGCGCTATTTACAGCTGCTGAGACCTATTTTTTCAATGAAGCCTGGATGACTGG

ORF Predictions :

ORF # Start End Direction Length

1 130 1029 F 300 aa

>[SEQ ID NO: 169] 3864898-1 ORF translation from 130-1029, direction F

VAALSQQDVPKALSCLNLLFDNGKSMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENL

ALPQKNLFEMIRLATVNLADIKSSLQPKIYAEMMTVRLAEIKPEPALSGAVENRIATLRQ

EVARLKQELSNAGAVPKQVAPAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIR

LQNAWGEVIESLGGPDKALLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNIL

SQAAGFSPEILAISMEEWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED* Description: unknown

Assembly ID: 3864938 Assembly Length: 167Obp

>[SEQ ID NO:72] 3864938 Strep Assembly -- Assembly id#3864938

CTGTCTCTGAAACAGTCACATCAAGTGCCTCTGAACAANCGCCCCNCCTAGGTNGACGGT

ATCGATAAGCTCGATCTGTGATTTCAGAGAAGAAATCAAGTGCTGTAACAGAAGTAAGAT

GTAATTGTATGTAAAGGAGACGTCATGTTAAATAGTATTGTAACCATTATTTGTATTGCC

CTTATCGCGTTTATCTTGTTTTGGTTTTTCAAAAAGCCTGAAAAATCTGGACAAAAAGCC

CAGCAAAAAAACGGATACCAAGAGATTCGAGTGGAAGTCATGGGAGGCTATACTCCTGAG

TTGATTGTCCTCAAGAAATCAGTGCCAGCCCGCATTGTCTTTGACCGCAAGGATCCTTCA

CCATGTCTGGATCAAATTGTTTTTCCAGATTTTGGTGTACATGCGAACCTGCCAATGGGG

GAAGAGTATGTAGTGGAAATCACGCCTGAACAGGCTGGAGAGTTTGGCTTTGCTTGTGGT

ATGAACATGATGCACGGCAAGATGATTGTAGAGTAGGTGGAGACTATGACAGAAATTGTG

AAAGCAAGCTTAGAAAATGGCATTCAAAAAATCCGTATCCGAGCTGAAAAAGGCTATCAT

CCAGCCCATATCCAGCTTCAAAAGGGAATTCCAGCTGAGATTACCTTTCATTCGTGCTAC

TCCTTCAAACTGTTATAAGGGAAATTCTGTTTGAAGAAGAAGGTATCTTGGAAGCAATCG

GCGTAGATGAGGAGAAAGTCATTCGTTTTACACCTCAAGAATTAGGGAGACATGAATTTT

CTTGTGGCATGAAGATGCAAAAGGGAAGCTATATAGTCGTTGAGAAGACTCGAAAATCTC

TATCTCTCCTGCAAACGTTTTTGGATTACTAGTATCTTTACTGTGCCTCTTGTGATTCTC

ATGATTGGGATGTTGGCAGGTAGCATTAGTCATCAAGTCATGCATTGGGGAACCTTTTTA

GCAACAACGCCTATTATGTTAGTTGCGGGTAAGCCATATATCCAGAGTGCTTGGGCCAGT

TTTAAAAAGCACAATGCCAACATGGATACCTTGGTTGCGCTGGGAACTCTAGTGGCTTAT

TTCTATAGCCTAGTTGCTCTCTTTGCTGGTCTCCCTGTTTACTTCGAAAGTGCTGGATTT

ATCCTCTTTTTCGTTCTTTTGGGAGCAGTTTTTGAGGAAAAAATGAGGAAAAATACGTCC

CAAGCTGTGGAGAAATTACTGGACTTGCAAGCTAAAACCGCAGAAGTCTTGAGTGATGAT

AGTTATGTCCAAGTTCCTTTGGAACAAGTCAAGGTACGCGACCTTGATTCCAGTGCGTCC

CGGTGAAAAGATTGCTGTTGATGGTGTCGTAGTAGAAGGTGTCTCTAGTATTGACGAATC

CATGGTGACAGGTGAGAGTCTGCCTGTGGACAAGACAGTTGGAGATACTGTCATTGGCTC

AACCATCAATCATAGTGGAACGCTTGTCTTTAGAGCAGAAAAAGTTGGCTCAGAGACTGT

TTTGGCTCAGATTGTAGATTTTGTGAAGAAAGCTCAGACAAGTCGTGCGCCGATTCAGGA

CTTGACGGATAAGATTTCAGGGATTTTTGTCCCAGTAGTTGTCATTTTAGGAATCATGAC

CTTTTGGGTTTGGTTCGTCTTGCTCAGGGATAGTGTGGTCGTGCTTGGAG

ORF Predictions :

ORF # Start End Direction Length

1 883 1326 F 148 aa

>[SEQ ID NO: 170] 3864938-2 ORF translation from 883-1326, direction F VPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQSAWASFKKHNANMDTLVAL GTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEKMRKNTSQAVEKLLDLQAKTA EVLSDDSYVQVPLEQVKVRDLDSSASR*

Description: ATCS_SYNP7

Assembly ID: 3864956 Assembly Length: 1252bp

>[SEQ ID NO: 73] 3864956 Strep Assembly -- Assembly id#3864956

ACAAGAACAATTGGAACAGGTACAGGCTGTTAAAAAATCGATTAACACAGCTAGTGAAGA

AGTGAAAAACCAAGTCTTGCTACCCATGGCTGATCACTTAGTGGCTGCTACTGAGGAAAT

TTTAGCGGCTAATGCCCTCGATATGGCAGCGGCTAAGGGGAAAATCTCAGATGTGATGTT

GGATCGTCTTTATTTGGATGCAGATCGTATAGAAGCGATGGCAAGAGGAATTCGTGAAGT

GGTTGCCTTACCAGATCCAATCGGTGAAGTTTTAGAAACAAGTCAGCTTGAAAATGGTTT

GGTTATCACAAAAAAACGTGTAGCTATGGGGGTCATCGGTATTATCTATGAAAGCCGTCC

AAATGTGACGTCTGATGCGGCTGCTTTGACTCTTAAGAGTGGAAATGCGGTTGTTCTTCG

TAGTGGTAAGGATGCCTATCAAACAACCCATGCCATTGTCACAGCCTTGAAGAAGGGCTT

GGAGACGACTACTATTCATCCAAATGTGATTCAACTGGTGGAGGATACTAGCCGTGAAAG

TAGTTATGCTATGATGAAGGCCAAGGGCTATCTAGACCTTCTCATTCCTCGTGGAGGAGC

TGGCTTGATTAATGCAGTAGTTGAGAATGCCATTGTGCCTGTTATCGAGACAGGAACTGG

GATTGTCCATGTTTATGTCGATAAGGACGCAGATGACGACAAGGCACTGTCTATCATCAA

CAATGCCAAAACCAGTCGTCCTTCTGTCTGCAATGCCATGGAGGTTCTGCTGGTTCATGA

AGACAAGGCAGCAAGCTTCCTTCCTCGCTTGGAGCAAGTGCTGGTTGCAGATCGAAAAGA

AGCTGGGTTGGAACCAATTCAATTCCGCCTAGATAGCAAAGCAAGCCAGTTTGTTTCAGG

TCAAGCTGCTCAAGCACAAGACTTTGATACCGAGTTTTTAGACTATATTCTAGCTGTTAA

GGTTGTGAGCAGTTTAGAAGAAGCGGTTGCGCATATTGAATCCACAGTACCCATCATTCG

GATGCTATTGTGACGGAAAATGCTGAAGCTGCAGCATACTTTACAGATCAAGTGGACTCT

GCAGCGGTGTATGTTAATGCCTCAACTCGTTTCACAGATGGAGGACAATTTGGTCTTGGT

TGTGAAATGGGGATTTCTACTCAGAAATTGCACGCGCGTGGTCCAATGGGCTTGAAAGAG

TTGACCAGCTACAAGTATGTGGTTGCTGGTGATGGGCAGATAAGGGAGTAAG

ORF Predictions :

ORF # Start End Direction Length

1 1030 1251 F 74 aa

>[SEQ ID NO: 171] 3864956-2 ORF translation from 1030-1251, direction F

VTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQKLHARGPMGLKELTS

YKYWAGDGQIRE*

Description:

106

SUBSTTTUTE SHEET (RULE 26) gamma-glutamyl phosphate reductase (proA) homolog - Haemophilus influenzae (str ain Rd KW20)

Assembly ID: 3864958 Assembly Length: 1785bp

>[SEQ ID NO:74] 3864958 Strep Assembly -- Assembly id#3864958

CTGCCCTAGCAGGAACGCAAGAAGGAACTGGAGAATAGGCATTTTCAAAATTATAACCTA

CACTAGCCATCATATCTAATGTTGGAGTGCTAACTAGCTTATCCTTACTATTCAAGGATA

AGGCGTCTGCTCTCATTTGATCTACAACAATCAAAATAATATTTGGTTGTTTTGTCTGAA

CCATAAAATCTCCTTTCTAATATGGCAAAAGAGGCACAAGAAGATATCTACCTTTACTGC

ACCCCTTTCTATATCAATCTCTCTATATAAAGCAATAACATTCTTGTTATGTTTTATAGA

ACAATGGACTAAAATATGACTAAATCGATTAGGAAATTCAAATCATTTTCTAGTACTGTT

TTAGTAAGTTACAGTGTACTATTCCAACTTCAATAAATTATAAACCTTTGTCTAATAACA

ATTTTAGTGGAGATAAGAAATCCTACACCTAACTCATCTTACACGTAATCTATTTCTATT

TTATCACAAAAAACGCAAGTAAGACCATTAACTCAATTCAGTTTTATCTGCCATTTTCAC

AAATGGGAAATAAGTCAAGACACTAATAATCAAACAAACAACTGATAAGATGATGGCACG

CCAATCAAATGCTGTAGAGAAGAAACCATATAAAATTGGAGGCATTACCCAAGTAACATT

TTGTGTAACAGGTGAAACAAGACCCCAGCTTGTTGCCCAGTAAGCTACCGTTGCCATGAA

AACCGGGCTAAGTACAAATGGTATAAATAGCAAAGGATTCAAGACAACTGGTAAACCATA

ATTCGATACCGGCTCACCAATATTAAACAGAACTGGTGCTAGACCAAGTTTAGCAACTTT

TCGATAATGACTGTTTCTTGAAAAAATTAAAATAGCAAGTACTAATCCTAATCCTCCAAA

CCAGACAAACGCCCCAAAAGACCCACTTGTCCATATATAAGGAATCGGTTCACCTTTTTG

GAAAGCATCCAGATTCGCTAACATAGCAACTCCAAATAGCCCTTCCATGATGGGAGCCAA

TACATTTCCTCCATGGAGACCAAAAAACCAGAATAACTTATTCAAAAAGATCATCAGAAT

AACTGCAAAGAAACTTTGAGACAAACCTAGTAATGGCGTTTGTAACACCTTGTAAACCCA

ATCAATCAATAAGTCATTGCTAAGTAAATGGAAAACATAAGTCAAGATGGCTACTATATA

CATCGCCATAAATCCTGGAATGATAGAAGTGAACGGCTTAGCAATCGCAGGGGGAACTGA

ATCTGGTAACTTGATTACCCAGTTCTTTTTCATTACTTTACAGAAAATAATAGAGGCTAA

AAATCCAATCATCATGGCTGTAAAGTAGCCTCTGGCATTAATATGGTTTCCTGGAATCAC

ATTCCCAATAGTTACCATCAGATTTTTACCATCAAATGCTAGATTATCAATTCCATGTTA

AGATTTGATCTAATTTCACATCTCCTACATTTGCCAAAGGGAAACTCTTTGTAACTGTAC

TTCCAATCGAAATGACAAACGAAGCAAGTGATACCAAACCAGCAGAAACTGTATCAACCT

TGTAAATCTTAGCGATATTCACTCCCAAGCAATAGATGAACAACAAGGAAACAATTGGTA

TACTTCCCTTGAATACCAAATTATTGATGTCAACAAGCCACTGAAAGGTTTTCGTAATAC

TTCCTAGGTGAAATTGTTGTGGTAAATCCACTAGAAAAGCATTTAATAACAAAGCAATGG

AACCTGTCATAATAACAGGCATAGTCCCCACAAATGAATCACGTT

ORF Predictions:

ORF # Start End Direction Length

1 1427 1711 R 95 aa

107

SUBSTTTUTE SHEET (RULE 26) >[SEQ ID NO: 172] 3864958-2 ORF translation from 1427-1711, direction R

VDLPQQFHLGSITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLV

SLASFVISIGSTVTKSFPLANVGDVKLDQILTWN*

Description: unknown

Assembly ID: 3865022 Assembly Length: 1386bp

>[SEQ ID NO: 75] 3865022 Strep Assembly -- Assembly id#3865022

ATCGAATTTCATTTCTATTTCCTATTCCATTTTTATTCAAAAAATCAAAAAGCAAACTAG

AAAGCTGGTCGCTGGTGGTTCAAAACACTGTTTTGAGATTGTCAATAGAACTGACAAACC

CTGTAATATACCTGCATATATACATACGACAAGGCGATACTACCCTAGTTTGAAGAGATT

TTCGAAGAGTATTCATTTTTGTCTTTTACTTATTATACCATATTCACATAAAAAAACGAA

CATTCTTATCCTAAAAAATGCTCATTTTTCTTAAATTATCAATCTAAATCTGGTTTATAG

AAGGAACGATTATCCATAGCGAAGATTTTATTGGTCATCTCTCCTTTATCCACCAAAGCC

AGAGCTGTTGACATCATCATCATGCTTGCATCCAGATTGTCAATCATATGGATAATCTCT

GCCTCCATAATACGTGGACGGACTGGAATTTCCATATTCAAGCAAGCCGTGGTGGACTTG

AGGATGACATGACGAAGCAAAACGACTTCTTCCTTGGTATCATCGATGCCGAGTTCCATA

ACTGTCTTGGTAATTTCGCTATCAATGAGAGCGATATGTCCAAGAAGATTACCTCGCACT

GTGTACTCTGTCTGGTCTGGCCCCGTCAACTCGATAACCTTAGCTAAGTCATGCAGCATA

ATCCCCGCATAGAGCAGGCTCTTATTGAGCTGAGGATAAACTTCGCTAATAGCGTCTGCC

AAACGTACCATGGTCGCCGTATGATAAGCCAACCCCGTTTCAAAGGCATGGTGGTTGGTC

TTGGCGGCTGGATAGGAGTAGAATTCCTTATCATACTTGGTGTAGAGATTTCGGACAATC

CGTTGCCAGACAGGATTTTCAATTTTGAAAATCATTTGCGACATGTAGTCACGAATTTCC

TTGACATCAACTGGTGACTTGACCTTGAAATCAGCTGGGTCATTGGGTTCACCAGCTTGA

GGCAGGCGGAGAGTAATTTGATTGACTTGAGGGGTATTGTTATAAACTTCTCGGCGTCCT

TTCATGTGGACAACCTTACCTGCGGTAAAGGCCTCAATGTTATGAGGTTGGGCATCCCAG

AGCTTCCCATCAATCTCGCCACTATCATCTTGGAAGGTAAAGGCTAGGTAGTTTTTCCCA

GCTCGAGTTTGCCTCAGGTCAGCTGATTTGATTAGGTAAAAGCCTTCAAATAACTCATCT

TTTTTCATGTGACTAATCTTCATATTCTTCCTCATTTTCTTGAAAATGGAGTAGATCAAG

CGCAGGCTCACCTTCTGACAACTCAATGTGACGGAGCGTCCGCTCGATAGCTATGGTACG

ACGGTTTAATAATTCGATCAATATTGCCAGAGGCATGTTGGAGATGTTTTTGTGCCTTGA

CCAGAA

ORF Predictions :

ORF # Start End Direction Length

1 279 1271 R 331 aa

> [ SEQ ID NO : 173 ] 3865022 - 1 ORF translation from 279 -1271 , direction R VSLRLIYSIFKKMRKNMKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI

108

SUBSmUTΕ SHEET (RULE 26) DGKLWDAQPHNIEAFTAGKWHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVKSP VDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAYHTAT MVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIALIDSEI TKTVMELGIDDTKEEWLLRHVILKSTTACLNMEIPVRPRIMEAEIIHMIDNLDASMMMM STALALVDKGEMTNKIFAMDNRSFYKPDLD*

Description: gi 1710422 (U21636) cmp-binding-factor 1 [Staphylococcus aureus]

Assembly ID: 3865036 Assembly Length: 1167bp

>[SEQ ID NO:76] 3865036 Strep Assembly — Assembly id#3865036

CTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTTTCCAGTTTCAAAAAGGCTTAG

AAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAAGGCAGGTGTTCTCCTCTAC

GCAGTCACCATAGCAGCCATCTTTAGTCTTTTGTTGCAATTTTATTTGAACCGACAAGTC

GCCCACTATCAAGACTATGCTTTGAATAAAGAAAAATTGGTTGCTTTTGCTATGGCTAAA

CGAACCAAAGATAAGGTTGAGCAAGAAAGTGGGGAACAGGTTTTTAATCTAGGTCAGGTA

AGCTATCAAAACAAGAAAACTGGCTTAGTGACGAGGGTTCGTACGGATAAGAGCCAATAT

GAGTTTCTGTTTCCTTCAGTCAAAATCAAAGAAGAGAAAAGAGATAAAAAGGAAGAGGTA

GCGACCGATTCAAGCGAAAAAGTGGAGAAGAAAAAATCAGAAGAGAAGCCTGAAAAGAAA

GAGAATTCCTAGTCAATTCAACTATAATGCGTTGAATCCAGAATAGTCCACTGTAGTTTC

TAGAAAATTGCTGGAAATGGATGTTAAGCTCCAATTCATTTGTTTATATCTTATTTCAGT

CCACTATACTTTGTGCTAAATTAAAGATATGAAACATGATTTTAACCACAAAGCAGAAAC

TTTCGATTTCCCTAAAAATATCTTCCTCGCAAACTTGGTATGTCAAGCAGCCGAGAAACA

GATTGATCTTCTATCAGACAAAGAAATTTTAGATTTCGGTGGTGGCACGGGTCTATTAGC

CTTGCCCCTAACCCCTAGCCAAGCAGGCTAAGTCAGTCACTCTTGTAGACATTTCTGAGA

AAATGTTGGAGCAAGCTCGTTTGAAAGTGGAGCAGCAAGCAATCAAGAATATCCAGTTTT

TGGAGCAAGATTTACCGAAAAATCCCTTGGAGAAAGAGTTTGATTGCCTTGCTGTTAGTC

GGGTTCTTCATCATATGCCTGATTTGGATGCGGCTCTCTCACTGTTTCATCAACATTTGA

AGGAAGATGGGAAACTCATCATTGCTGATTTTACCAAGACAGAAGCTAATCATCATGGAT

TTGATTTAGCTGAACTGGAAAACAAGCTAATTGAGCATGGGTTTTTCATCTGTGCATAGT

CAGATNCTCTATAGCGCTGAAGANCTG

ORF Predictions :

ORF # Start End Direction Length

1 79 492 F 138 aa

>[SEQ ID NO: 174] 3865036-1 ORF translation from 79-492, direction F VWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNKEKLVAFAMAKRTKDKV EQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVKIKEEKRDKKEEVATDSSE KVEKKKSEEKPEKKENS*

109

SUBSTTTUTE SHEET (RULE 26) Description: unknown

Assembly ID: 3865054 Assembly Length: 916bp

>[SEQ ID NO: 77] 3865054 Strep Assembly -- Assembly id#3865054

TCTCCCAACATATAATTTCCGTTTTCCAATCCCCCAGCTGTCATACAGTCTGTGATAAGA

GCGATGTTTTCTGTTCCTTTTTGTTTGATAAGAATTTCGCAAGCCTTTGGATCTACGTGG

TGACCATCACAGATCAACTCTGCATAGGTATGTGGCAATTGGTACATGGCTCCAACCATA

CCCAATTCACGGTGAGTCAACCCACGCATTCCATTGTAGGCATGCACCCAAACACTCGCT

CCAGCATCGACTGCTTTTTTGGCTTCATCAAAAGTCGCGTTTGAATGTCCAAGAGCAACC

GTCACACCTTCGCCCGTAACTGTACGAACAAAGTCTTCCACCCCATCACGTTCTGGTGCA

ATCGAATTTTATTAAGCAAGCCATTTGCCGCTTTTTGCCAAGAATGAAACTCCTCAACAC

CCGGGTCTCTCATATAAGTTGGATTTTGTGCCCCCTTAAAAGTTTCTGTGAAATATGGAC

CTTCATAATAAATCCCACGAATCTTAGCACCTGTTGCTTCTTTATAATGGTTTCCAAGAT

TTTCAGTGACTGCAAGCAATTGCTCATAAGTGGCTGTTAAAGTTGTGGGTAAGAAACTGG

TAACACCGGTACTAAGAAGTCCTTCACTCATAGTATGCAATGTACCTTCAATGTTGTTGT

CCATCACATCTACACCTGCATATCCATGAATATGAGTATCCACAAGACCTGGGGCAATGC

TATAACCTGTATAGTCAATCACCTCAGCCCCTTCAGGAATCTGCTCTACATGTTTCCCAA

ACTTGCCGTCCACAAGTTCCAAGTAACCACCTCGACAAATCCGTGTGGGTAGAAAAACTG

ATCCGCTTTAATATAGTTAGGCATAATGTTAACCTCCTTAAAAGATTGATTCTACAATTT

ATTATGTCAATTCGAT

ORF Predictions :

ORF # Start End Direction Length

1 302 793 R 164 aa

>[SEQ ID NO: 175] 3865054-1 ORF translation from 302-793, direction R VDGKFGKHVEQIPEGAEVIDYTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLL STGVTSFLPTTLTATYEQLLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTY MRDPGVEEFHSWQKAANGLLNKIRLHQNVMGWKTLFVQLRAKV*

Description:

N-acetylglucosamine-6-phosphate deacetylase (nagA) homolog - Haemophilus influe nzae (strain Rd KW20)

Assembly ID: 3865102 Assembly Length: 786bp

>[SEQ ID NO:78] 3865102 Strep Assembly -- Assembly id#3865102 CTGGATTAAAACGAGGCAGTTTCAGACTAATATCCAAGTCGTAAGAAATGCCTGAAATAA GCTTTTCTAAATTGTCCAAAGCTTGCGGGAAAACGCTCTTGGAATAGTTTCTCTAAAGAA CTTGCTGATATAAAGACATCTTGTCTCGAACGCAAGGGAACTTCTCTGAGCGGTAGATTT TCTTTAATCGCTGTTAAAACTTGAAGAACTTCTCTATCCCTGCTTTCAAAAGCGTTGACC CGATAAAGAGGTAAGATAGGATGATGAAATTCGCTTGCTAGTGTTTCTGGATAAACCCCT ATATAGTAATCACAGCCTAGTTCTAACGACTCAACTCTATCAAAATAAGGCACAATGACC GCGATATCCTCCAGGTACTGGGACAGGACTGACCAAGTTTTCTCCCCCTGCATCTTGGCT GTCGAAAGCTTCATCAACTGCTGATAGCCCACACTAGATAGAGCTAAAAAGCGCAAATTC ACTTCCTGATCATCTACAAACACTGTCATTTCAAGCCCTAGCAAAGGATGAATGCCGTAT TTTTTTGTAATCTCTAGAAAGTCGAAAGCGCCATAAAGATTGTCAATATCCATCATAGCC AAATGAGTGTAGCCGTATTCTTTAGCTGCTCTCACATACTTTTCGATCGAAATGACGCTT TCCATAAAACTATAGACTGTTTTTGTATCTAGTTGTGCGATCAATTTACACTTCTCCTCT ATCCTTCTCACTATATTATACCATTTTCACCTATAAATGGCTTCTCTTGAGAAAAATTTC GATCAG

ORF Predictions :

ORF # Start End Direction Length

1 27 731 R 235 aa

>[SEQ ID NO: 176] 3865102-1 ORF translation from 27-231, direction R

VRRIEEKCKLIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLYGAFDFLE

ITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEKTWSVLSQYL

EDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFESRDREVLQVLT

AIKENLPLREVPLRSRQDVFISASSLEKLFQERFPASFGQFRKAYFRHFLRLGY*

Description: unknown

Assembly ID: 3865156 Assembly Length: 1213bp

>[SEQ ID NO: 79] 3865156 Strep Assembly -- Assembly id#3865156 CACTTTCAGCTTCTTCTCTTTTTGAACGGTTATAAACACGAATCAGATTCCCTATTTCTT GCGATTTATGTGATTCCTTATTTTCCAATCTAAAGTATAGTGAAATGAAATAAAACATGC GCAAATCGATTAAGGAATTTAATCTAATTTCTAACAATGTCTTAGAAATCAAAGTGTACT ATTTTAACTTCAATGCACTAAACATCTAATACTCAATAAAAATCAAAGAGCAAACTAGGA AACTAGCCGCAGGTGGCTCAAAACACTGTTTTGAGGTTGTAGATGAAACTGACGAAGTCA GTAACCATACATACGGCAAGGCGACGCTGACGTGGTTTGAAGAGATTTTCGAAGAGTAGC AAAATGGAAAAAGGAGTGAGTGAAGCACATCGCCTCCCCACTCCTTTTTCTGTTTTTAGG CTGTTTTTTCAACCTTCAAGATTTTTACATCATAGCTACCAACAGGCGTTTCAATGGTTG CTGTATCACCTGTTTTCTTGCCAATCAAGGCCTGCCCAATTGGGCTTTCATTTGAAACCT TACCTGCAAAGGCATCCGCACCAGCTGAACCTACGATAATATAAACTTCTTCTTCGTCCT CACCAATTTCTTGGATGGTGACTGTTTTACCAATCGCTACTTCGTCCTGGGCAACTGCGT

111

SUBSTTTUTE SHEET (RULE 26) CGCTATTGACGATTTCAGCATAGCGGATTTTTGTTTCTAAGCTAGAGATTTGTCCTTCGA CAAAGGCTTGTTCATCCTTAGCTGCTTCGTACTCACTGTTTTCTGAAAGGTCACCGTATG AACGGGCAATCTTAATGCGTTCTACCACTTCTGGTCGACGAAACCAATTTCAATTCTTCT AATTCTTTTTCAAGTTTTTCCTTTTCCTCAAGGGTCATAGGATATGTTTTTTCTGCCATT TTTCTCAACTTTCTTCTGATAATATTTTCTAAAGAAAATTATGTGAAGTATCACATAATT TTAGTTTGTTTAGTTTAATTTGCTGTTGACATGTTCAGCGACATTGCGGTCGTGGTCTTC TTGATTGTTAGCATAGTAAACCTTGCCTTCTGTGACATCTGCTACAAAGTAAAAGTTATC GCTCTTAGTTTGATTGATGCTTGACTCAATCCGCATCCAAGACTTGGACTATCGACTGGA CCAGGCATGAGACCTACATTTTTATAAACATTATAAGGTGAATCAATGTTGGTATCAATC GCAACATCCTCAG

ORF Predictions :

ORF # Start End Direction Length

1 416 808 R 131 aa

>[SEQ ID NO: 177] 3865156-1 ORF translation from 416-808, direction R WERIKIARSYGDLSENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIG KTVTIQEIGEDEEEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYD VKILKVEKTA*

Description:

TRANSCRIPTION ELONGATION FACTOR GREA (TRANSCRIPT CLEAVAGE FACTOR GREA) . - ESCHE RICHIA COLI .

Assembly ID: 3865160 Assembly Length: 1173bp

>[SEQ ID NO: 80] 3865160 Strep Assembly -- Assembly id#3865160

TGCGGCTGAGTTGGGAATTCCTATCGTTAATAAGCGTGTATCGGTGACACCTATTTCTCT

GATTGGGGCAGCGACAGATGCGACGGACTACTGGTTCTGGCAAAAGCGCTTGATAAGGCT

GCGAAAGAGATTGGTGTGGACTTTATTGGTGGTCTTTCTGCCTTAGAACAAAAAGGTTAT

CAAAAGGGAGATGAGATTCTCATCAATTCCATTCCTCGCGCTTTGACTGAGACGGATAAG

GTCTGCTCGTCAGTCAATATCGGCTCAACCAAGTCTGGTATTAATATGACGGCTGTGGCA

GATATGGGACGAATTTATCAAGGAAACGGCAAATCTTTCAGATATGGGAGCGGCCAAGTT

GGTTGTATTCGCTAATGCTGTTGAGGACAATCCATTTATGGCGGGTGCCTTTCATGGTGT

TGGGGAAGCAGATGTTATCATCAATGTCGGAGTTTCTGGTCCTGGTGTGGTGAAACGTGC

TTTGGAAAAAGTTCGTGGACAGAGCTTTGATGTTAGTAACCCGAAAACCAGTTAAGAAAA

CTGCCTTTTAAAATCACTCCGTATCCGGTCCAATTGGTTTGGTCAAATGCCCAGTGAGAG

ACTGGGTGTGGAGTTTGGTATTGTGGACTTGAGTTTGGCACCAACCCCTGCGGTTGGAGA

CTCTGTGGCACGTGTCCTTGAGGAAATGGGGCTAGAAACAGTTGGCACGCATGGAACGAC

AGCTGCCTTGGCCCTCTTGAACGACCAAGTTAAAAAGGGTGGAGTGATGGCCTGTAACCA

GGTCGGTGGTCTATCTGGTGCCTTTATCCCTGTTTCTGAGGATGAAGGAATGATTGCTGC

112

SUBSTTTUTE SHEET (RULE 26) AGTGCAAAATGGCTCTCTTAATTTAGAAAAACTAGAAGCTATGACGGCTATCTGTTCTTG TTGGATTGGATATGATTGCCATCCCAGAAGATACGCCTGCTGAAACTATTGCGGCTATGA TTGCGGATGAAGCAGCAATCGGTGTTATCAACATGAAAACAACAGCTGTTCGTATCATTC CCAAAGGAAGAGAAGGCGATATGATTGAGTTTGGTGGTCTATTAGGAACTGCACCCGTTA TGAAGGTTAATGGGGCTTCGTCTGTCGACTTCATCTCTCGCGGTGGACAAATCCCAGCAC CAATTCATAGTTTTAAAAATTAAGAAAATAGGA

ORF Predictions :

ORF # Start End Direction Length

1 136 375 F 80 aa

>[SEQ ID NO: 178] 3865160-1 ORF translation from 136-375, direction F

VDFIGGLSALEQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTAVADMGRI

YQGNGKSFRYGSGQVGCIR*

Description: unknown

Assembly ID: 3865172 Assembly Length: 1209bp

>[SEQ ID NO: 81] 3865172 Strep Assembly -- Assembly id#3865172 TCGGAATCTGAGCTAGTGTAGCTTCCTTAATCTTATCTGATAAGATAGCTGTCATATCAG ACTCAATCATTTCCTGGAGCAATCAACATTGACTCGTATATTCCGACTAGCGACCTCGCG TGCCACAGACTTGGTAAAGCCAATCAAGCCAGCCTTAGAAGCAGCATAGTTAGCTTGACC AATATTCCCCATCAAACCAACAACACTAGACATATTAATGATAGCACCTTCTCTGGCTTT CATCATCGGTTTCAAGACTGATTGTGTCATATTAAAGGCACCAGTCAGATTGACCTTGAG CACTTTTTCAAAATCTGCTTCTGTCATCTTGAGCATAAGAGTATCTTGGGTAATCCCTGC ATTGTTGACCAAAACATCTACTGAACCCAGTTCTGCAATAGCTTGATCAATCATACGCTT AGCGTCTGCAAAATCTGATACATCTCCTGAAATGGGAACCACCTTGATACCATAGTTTGA AAACTCAGCGAGCAATTCTTCTGAGATTGCCCCACGACTGTTTAAGACAATGTTGGCTCC TGCTTGAGCAAACTTGTGGGCGATGGCAAGACCAATTCCACGACTCGAACCTGTAATAAA GATATTTTTATGTTCTAGTTTCATTTTTTTCCTTTCAAAACTTCTACTTATTTTAGTCTA TTTTTCTAAAAGTGCTACTAAACTCGCTTGATCTTCCACATGAGCTAAGTGAGCAGTTTG ATCAATTTTTTTAACAAAACCTGACAAGACTTTCCCCGGTCCAATCTCGAATAAAGTTGC TTATGCCTGCTTCTTGCATGACCCCAATACTTTCATAGAAACGAACGGGTTCCTTGACCT GACGCGTCAAGAGCTGAGCAATGTCCTCTTTTTGCATCACAGCAGCTTCTGTATTGCCGA CTAGGGGACAAGTAAAATCTGAAAAACTTACCTGAGCTAGAGTTTCAGCTAGTTTCTGGC TAGCAGGCTCAAGGAGAGCGGTGTGAAAGGGACCTGACACCTTAAGAGGAATCAAGCGTT TGGCACCTGCTTCTTGCAAAAGTTCAACCGCTCGATCAACTGCAACCACTTCTCCAGCAA TGACGATTTGTGCAGGTGTGTTATAGTTGGCTGGAGTAACCACTCCAAGTTCCAGAAGCT TTTTGACAGGCTTCTTCAATGACCTCTACTGGCGTATTGAGAACTGCTACCATCTTGCCA AGTTCAGCA

ORF Predictions :

ORF # Start End Direction Length

1 731 1123 R 131 aa

>[SEQ ID NO: 179] 3865172-2 ORF translation from 731-1123, direction R WTPANYNTPAQIVIAGEWAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAE TLAQVSFSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIRD WTGESLVRFC*

Description: malonyl coenzyme A-acyl carrier protein transacylase (fabD) homolog - Hae ophil us influenzae (strain Rd KW20)

Assembly ID: 3865228 Assembly Length: 813bp

>[SEQ ID NO: 82] 3865228 Strep Assembly -- Assembly id#3865228

ATGACACGTCTGTTCTCTCAAGCAGAAATGGCAGAGTAACAAGCTCGATATTGAGGTAGC

CGATAAAGAATTGGCTGAATTTGAAGCTCAGATTAAACAGGAAGTGGAAGCTCCAACTTG

TAGTGAGTCCTCAGGTTGAAGAAGAGCCTCAGCTCATCCAGTTGGCCCAATGTATGAAGA

ACCAGAAGTAAATCCAGTGCATCCGACAGGTCCAACACCAGCTACAGAAACTGTTGATTC

AATACCGGGATTTGAAGCACCGCAAGAATCTGTTACAATTTTATAAGAAATATTCTGAGA

ACAATATCTTATCCTTATATTTCCAGCGAGCAGGAAATGGTGTGAGTCCTGCATTCCCTA

TCGATAAGATTATCCTCTCAAACTATCAAGTCTGAATCTAGTAAGATTTGACGTTCCCCA

CGTTACGGGATAAGAGAGAGAAAGACTAAATCTTTTTCCGAATAAAGGTGGTACCACGAT

TTTCGTCCTTTTTGGAAGTCGTGGTTTTTAATTTGTTATTATTTATAAAGGAGATACCAT

GAAACTCAAAGACACCCTTAATCTTGGGAAAACTGAATTCCCAATGCGTGCAGGCCTTCC

TACCAAAGAGCCAGTTTGGCAAAAGGAATGGGAAGATGCAAAACTTTATCAACGTCGTCA

AGAATTGAACCAAGGAAAACCTCATTTCACCTTGCATGATGGCCCTCCATACGCTAACGG

AAATATCCACGTTGGACATGCTATGAACAAGATTTCAAAAGATATCATTGTTCGTTCTAA

GTCTATGTCAGGATTTTACGCGCCATTTATTCC

ORF Predictions:

ORF # Start End Direction Length

1 197 286 F 30 aa

>[SEQ ID NO:180] 3865228-1 ORF translation from 197-286, direction F VHPTGPTPATETVDSIPGFEAPQESVTIL*

114

SUBSTTTUTE SHEET (RULE 26) Description: unknown

Assembly ID: 3865230 Assembly Length: 953bp

>[SEQ ID NO: 83] 3865230 Strep Assembly — Assembly id#3865230

ATCGAATTATTTTGAAACAAGGTGGATCAGCTATTTTGGCCTTGATTAGTATTTTACTCT

TTAAATACACTTGAAGGTCGATTCTAATCTCGCTAATCCTTTTTAATCCAGAATAAGGGA

AATATGTTATACTTGTTTTTAAGAAAAAAGTTTCATTGAATTGGTTTTGAGGAGTTAGAA

ATGAAAGTATTAGTGACAGGTTTTGAGCCCTTTTGAGGCCATTAAAGGTTTACCAGCTGA

AATCCATGGTGCTGAGGTCCGTTGGCTAGAGGTGCCGACAGTTTTTCACAAATCTGCTCA

AGTATTGGAAGAAGAGATGAATCGTTATCAACCTGACTTTGTCCTTTGTATTGGGCAAGC

TGGTGGAAGAACTAGTTTGACACCTGAACGAGTGGCCATTAATCAAGACGATGCACGTAC

TTCTGATAACGAAGATAATCAACCGATTGACCGTCCCATTCGCCCAGATGGTGCTTCGGC

CTACTTTAGTAGTTTGCCGATTAAAGCGATGGTTCAAGCTATAAAAAAGAAGGATTACCG

GCCTCTGTTTCCAATACGGCAGGGACTTTTGTCTGCAGCCATTTGATGTATCAGGCTCTC

TATTTGGTAGAAAAGAAATTCCCATATGTTAAGGCAGGTTTTATGCATATTCCTTATATG

ATGGAACAGGTGGTGAACAGACCGACTACTCCAACTATGAGTTTAGTGGATATTCGGCGA

GGGATAGAAGCAGCAATCGGCGCTATGATAGAACATGGAGATCAGGAACTCAAGTTGGTA

GGCGGAGAAATTCATTGATAGAAAAAAGCTTGAGGGGAAAACCTTCAAGCTTTTGGACGT

TTTCGAGCCAATACTGCTCGGTAAAACATAATTTTAGTGCATTGGATATAAGGTAGGAGT

GAAAAACTAGCAATGCCAAAGGTAATCCAATTGAGGAAGTACCAAGGAAGAAG

ORF Predictions:

ORF # Start End Direction Length

1 272 586 F 105 aa

>[SEQ ID NO:181] 3865230-1 ORF translation from 272-586, direction F

VPTVFHKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPID

RPIRPDGASAYFSSLPIKAMVQAIKKKDYRPLFPIRQGLLSAAI*

Description:

PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) . - STR EPTOCOCCUS PYOGENES .

Assembly ID: 3865378 Assembly Length: 1060bp

>[SEQ ID NO: 84] 3865378 Strep Assembly -- Assembly id#3865378

CTACTTGAAACAGAACTGAAATTATACCCACTACCTCCCTGATTATCTTCAATGCTTACG

TCTAAATAAACTTCCCCACTATTATTTAGCTTAGCAACAACTGTTATAGTAAAATAACAT

115

SUBSTTTUTE SHEET (RULE 26) AAAATTCACATAAATAGATTAGGGAAATCAAAGCAACTTCTAGGAATGTTTTAGCAGTCA CAGTGTACTTTCCCAGCATCAAGCCACTATAACTCTGCACATAAAAATGGAGAAGATGGC CATCCTCTTCTCCAAATATTAACTTCTTTACAAACCAACTATAGTTGACAAAGAACCTAA AATCAATTGATAACACGAGGTCAGGTCGGTCAACTCTTTCAACTGAAGCCCTGTCAACTC TTCCCATTTATCAATCTTGTATTGGAGAGAATTGCGGTGCAGATAGAGTTGCTGGGCTGT TTAAGTGAGAACAGCACTATTTTCCCAAAGAGAGAGAATGATTTCCTGAATCTGATCTTG ATCCAAAATCATCTGGTGTAGACATTCCTTGATTGGCTTCAAGTCCACGAGTCTTTCTCC CAGACTCCAAAGATAGAGCTGAGAAAAAGTATGAACACCTTGGTGACCCTGACGCCACCA TGTCTTGAACAAATCCCGCTCAGCTTTGATTAAGTCTGATAGGGCTTGATGTCCCGTCTG AGACCAAACCTGACCCAACATGATAGAAAGACGAAGTCCAAAGTCATACTCAACCGCTTC AATCGTATCACTTAAAATATCTCTTACAGAAGTGTATTTGTCTTGTTGAAGCACGAAAAC ATAATCCTGAGATCCGACCTGTAGCACTGTCTGACAATTCGGAAAAAGAGTCCGCATCAT ATCTAGCCAAGAAGCCAGATTTTCCTGCTGAAAATAAGAAAGATGGCAATAAACCAACTG AATCTTTTTAAAAACTTGCGGTGCCTGTCCCTTGCCTTCAACCAGATAGGAATACCAAGG GTTTAGCGAACGAACCTGCTCCTGCTGGGTCAAAAGGGCAACCAACTGCTTTTCACGCTC GCTGAGCCCAGCTTCCTCCAGCAAAATCCACTGCTGAGAG

ORF Predictions:

ORF # Start End Direction Length

1 421 807 R 129 aa

>[SEQ ID NO:182] 3865378-1 ORF translation from 421-807, direction R VLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLGQVWSQTGHQALSDLI KAERDLFKTWWRQGHQGVHTFSQLYLWSLGERLVDLKPIKECLHQMILDQDQIQEIILSL WENSAVLT*

Description: unknown

Assembly ID: 3865470 Assembly Length: 895bp

>[SEQ ID NO: 85] 3865470 Strep Assembly -- Assembly id#3865470

ATTTTAGACTTTGATGACAATCCTCAGGCGGTTATCATGCCCAATCACGAGGGGCTGGAA

TTGCAGTTGCCAAAGAAGTGTGTTTATGCATTTTTAGGTGAGGAGATCTGACCGCTATGC

AAGGGAAGTAGGGGCGGATTGTGTCGGCGAATTCGTTTCTGCTACCAAGACCTATCCAGT

CTCTTTCATCAACTACAAGGGTGAGGAGGTCTGTCTGGATCAGGCTCCTGCTGGCTCCGC

TCCAGCAGCCCAGTTTATGGATGGGTTGATTGGCTATGGTGTGGAGCAGCTTATCTCTAC

TGGGACCTGTGGTGTCCTAGCTGATATAGAGGAAAATGCCTTTCTAGTCCCTGTTCGCGC

TTTGCGAGATGAGGGAGCCAGTTACCACTATGTGGCACCTTGTCGTTATATGGAAATGCA

GCCAGAGGCTATTGCTGCTATTGAGGAAGTTTTGGAAGACAGAGGGATTCCTTATGAAGA

AGTCATGACCTGGACGACAGACGGTTTTTACCGAGAAACGGCTGAAAAGGTGGCTTATCG

116

SUBSTTTUTE SHEET (RULE 26) TAAGGAAGAAGGCTGTGCTGTTGTGGAGATGGAGTGTTCTGCTCTTGCGGCAGTAGCTCA ATTGCGTGGGGTTCTCTGGGGTGAATTGTTGTTCACAGCAAATTCTCTAGCGGACTTGGA CCAGTACAACAGTCGTGACTGGGGCTCGGAACCTTTTAATAAGGCGCTAAAACTGAGTTT AGCAAGTGTCCACCACCTTTAGTTGTACTGGCAAAGGATTTGTTTTATCATAAAATGTCT AGCTCATACTTTTCAAAAATATGTTTAAACGAAGTCACCTTCCTCTTGTCCTAAGCATGT TTGAAGTTGGGAAAAATCTTTAAAATCAGAAAAACGTATCATATCAGGTTGATGA

ORF Predictions :

ORF # Start End Direction Length

1 98 742 F 215 aa

>[SEQ ID NO: 183] 3865470-1 ORF translation from 98-742, direction F

VRRSDRYAREVGADCVGEFVSATKTYPVSFINYKGEEVCLDQAPAGSAPAAQFMDGLIGY

GVEQLISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE

DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAWEMECSALAAVAQLRGVLWGELLFT

ANSLADLDQYNSRDWGSEPFNKALKLSLASVHHL*

Description: unknown

Assembly ID: 3865632 Assembly Length: 645bp

>[SEQ ID NO: 86] 3865632 Strep Assembly -- Assembly id#3865632

AGGGCTGTCAAGCTTGGTTAGAACGTTTAGAAAAGGAGAGTTAAGGTGGAAAATCTTACG

AATTTTTACGAAAAGTATCGTGTCTATCTGACTCGTCCACGTTTAGAGCTTTTGGCAGTA

GTTACCATTGTTTTANGNGCTGTACTCGTCTTTTTTCTAAATATTCCAGGAAAAGGTGTC

TTAAAACTCGATAATGGAACGATTGTTTATGATGGCAGTCTTGTCCGTGGTAAAATGAAT

GGCCAAGGTACCATTACCTTCCAAAATGGAGACCAATATACAGGTGGCTTCAACAATGGA

GCCTTCAACGGAAAAGGTACCTTTCAATCTAAAGAAGGCTGGACCTACGAAGGTGATTTT

GTAAATGGTCAGGCTGAAGGAAAAGGGAAACTAACAACAGAACAAGAAGTCGTTTATGAA

GGAACTTTTAAACAAGGCGTTTTTCAACAAAAATAAAGCCTCCTTATCAAAGGAGGTATT

ATTAGAATTACAAGGTAAGCGTTTACCTGTAAATCCCTTTCTTTCCAAATCCCTCTTCCA

AGCAAGTTTGTGAAATAAAAAATATTTGAAATAAATTTCACAAACTTCAAAGATAAAACC

TGATAAGAAAAGAAAATGAGAAAAGTTTCGCAAGAGTTTAAAAAT

ORF Predictions:

ORF # Start End Direction Length

1 46 456 F 137 aa

> [ SEQ ID NO : 184 ] 3865632 -1 ORF translation from 46-456 , direction F

117

SUBSTTTUTE SHEET (RULE 26) VENLTNFYEKYRWLTRPRLELLAWTIVLXAVLVFFLNIPGKGVLKLDNGTIVYDGSLV RGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYEGDFVNGQAEGKGKLTTEQ EWYEGTFKQGVFQQK*

Description: unknown

Assembly ID: 3865710 Assembly Length: 572bp

>[SEQ ID NO: 87] 3865710 Strep Assembly -- Assembly id#3865710

GAGATCTGTCTTGACACCAAAAGTGTGGAGTACGCCAGCTAATTCAACGGCGATATAACC

AGCGCCTAGAATCGCAATTGACTCTGGAAGTTCTTCCCAGGCAAATACATCATCAGAAGA

GCCACCTAGCTCAGCACCAGGAATATTAGGAATACTTGGATGGGCACCTGTAGCAATCAC

GATATGTCTAGCACGAATCAGTTCACCATTTACGCTTACAGTATGAGAATCTACAAATTC

AGCATGACCTTCAATCAAGTCTACACCGTTGCGTTTAAAACTACCATCATAGAGAAGAAC

GAGCGCGATCAATGTAGGCTTCACGATTGCGACGTAGGGTTGCAAAGTTAAAGTTAAGAT

CAGTAGTCTCAAAGCCGTAGTCTCCTCCAAATTGATGGAAAGTCTCAGCGATTTGCGCCC

CGCTACCACATGATTCTTTTAGGAACACAACCGACGTTGACACAGGTTCCACCTAATTTC

TTTTCCTCAATAACGGCTGCTTTGGCTCCATGTTCCCAGCACGGTTCATGGTAGCGATCC

TCCGCTACCTCCACGATAGCAATGATATCATA

ORF Predictions:

ORF # Start End Direction Length

1 287 448 R 54 aa

>[SEQ ID NO: 185] 3865710-1 ORF translation from 287-448, direction R VFLKESCGSGAQIAETFHQFGGDYGFETTDLNFNFATLRRNREAYIDRARSSL*

Description: glutathione reductase (NADPH) (EC 1.6.4.2) - Streptococcus thermophilus

118

SUBSTTTUTE SHEET (RULE 26) Provided in Table 2 is information on the direction of the ORF (forward or reverse) for each polynucleotide in Table 1. Also listed for each ORF is its start and stop codon positions (refer to the columns containing nucleotide code labeled "Start" and "Stop"). The triplet codon sequence for each start and stop codon is also shown. These codons may be shown in the sense orientation or antisense orientation, such as GTG and CAC, respectively, for start codons. The "Length" column discloses the length of each polynucleotide assembly. The direction of translation on the polynucleotide depicted is denoted by and "Forward" for forward or and "Reverse" for reverse (or being on the opposite strand from the one depicted). As indicated above, the "Assembly ID" number is a unique identifier assigned to each ORF of Table 1 and allows a correlation between the data in Tables 1 and 2.

TABLE 2

Assembly Start Stop Start Stop Length Direction ID

3049156 ~CAC TCA~ 236 385 50 Reverse 3049862 GTG TGA 383 526 48 Forward 3112810 -CAC TTA~ 601 804 68 Reverse 3112866 ~CAC TTA~ 220 513 98 Reverse 3113664 GTG TAA 165 392 76 Forward

119

SUBSTTTUTE SHEET (RULE 26) Assembly Start Stop Start Stop Length Directioi

3113716 -CAC TTA- 94 291 66 Reverse

3174176 GTG TAA 139 543 135 Forward

3174186 GTG TAG 83 283 67 Forward

3174374 GTG TGA 154 294 47 Forward

3174972 -CAC TTA- 169 678 170 Reverse

3175138 -CAC TCA- 79 945 289 Reverse

3175860 GTG TAA 51 251 67 Forward

3175918 GTG TGA 212 535 108 Forward

3811220 -CAC CTA- 316 873 186 Reverse

3811436 -CAC TTA- 1164 1511 116 Reverse

3811984 GTG TGA 134 454 107 Forward

3857228 -CAC TCA- 1141 1356 72 Reverse

3857842 GTG TAA 45 341 99 Forward

3857996 GTG TAA 58 456 133 Forward

3858236 -CAC CTA- 1 261 87 Reverse

3858264 -CAC TCA- 439 1365 309 Reverse

3858610 -CAC TTA- 374 949 192 Reverse

3858716 -CAC CTA- 238 402 55 Reverse

3859124 -CAC CTA- 73 453 127 Reverse

3859244 -CAC TTA- 310 462 51 Reverse

3859250 -CAC CTA- 244 402 53 Reverse

3859588 -CAC TTA- 102 443 114 Reverse

3859774 -CAC CTA- 9 131 41 Reverse

3860140 GTG TAA 302 511 70 Forward

3860140 GTG TAA 605 856 84 Forward

3860206 -CAC TTA- 898 1056 53 Reverse

3860270 GTG TAG 346 966 207 Forward

3860438 GTG TAG 1 276 92 Forward

3860438 GTG TGA 460 1128 223 Forward

3860544 GTG TAA 222 689 156 Forward

3860558 -CAC TTA- 717 1376 220 Reverse

3860568 GTG TAA 1040 1291 84 Forward

3860582 GTG TGA 356 1027 224 Forward

3860724 GTG TGA 139 498 120 Forward

120

UBSTTTUTE SHEET (RULE 26) Assembly Start Stop Start Stop Length Directioi

ID

3860724 GTG TGA 686 1024 113 Forward

3860858 GTG TAG 610 807 66 Forward

3860890 GTG TAG 397 486 30 Forward

3860952 -CAC TTA- 449 715 89 Reverse

3860962 -CAC TTA- 152 646 165 Reverse

3861268 -CAC TTA- 457 645 63 Reverse

3861270 -CAC TTA- 627 824 66 Reverse

3861288 -CAC CTA- 357 572 72 Reverse

3861306 GTG TAA 717 1208 164 Forward

3861306 GTG TAA 1201 1410 70 Forward

3861334 GTG TAA 76 975 300 Forward

3864148 GTG TAG 212 940 243 Forward

3864148 GTG TAA 1202 1753 184 Forward

3864148 GTG TAA 2750 3037 96 Forward

3864172 GTG TAG 311 862 184 Forward

3864180 -CAC TTA- 930 1616 229 Reverse

3864184 GTG TGA 197 670 158 Forward

3864184 GTG TAA 612 1304 231 Forward

3864194 -CAC CTA- 1084 1380 99 Reverse

3864338 GTG TGA 552 1100 183 Forward

3864360 GTG TAA 47 1078 344 Forward

3864388 GTG TGA 1239 1586 116 Forward

3864406 -CAC TTA- 263 958 232 Reverse

3864452 -CAC TCA- 1079 1201 41 Reverse

3864458 GTG TAA 797 1105 103 Forward

3864458 GTG TGA 1179 1391 71 Forward

3864474 -CAC CTA- 68 247 60 Reverse

3864474 -CAC TTA- 644 1528 295 Reverse

3864510 -CAC TTA- 1164 1640 159 Reverse

3864526 -CAC TTA- 845 1660 272 Reverse

3864548 GTG TGA 687 1055 123 Forward

3864548 GTG TAA 979 1932 318 Forward

3864582 -CAC TTA- 317 550 78 Reverse

3864604 -CAC CTA- 1 141 47 Reverse

3864604 -CAC CTA- 1513 1803 97 Reverse

3864610 GTG TAA 427 1305 293 Forward

3864716 GTG TAA 57 272 72 Forward

3864718 GTG TGA 77 1474 466 Forward

3864802 -CAC TTA- 92 550 153 Reverse

121

BSTTTUTE SHEET (RULE 26) Assembly Start Stop Start Stop Length Directioi

ID

3864854 -CAC CTA- 324 548 75 Reverse

3864862 -CAC CTA- 431 1003 191 Reverse

3864888 -CAC TTA- 10 657 216 Reverse

3864898 GTG TAA 130 1029 300 Forward

3864938 GTG TGA 883 1326 148 Forward

3864956 GTG TAA 1030 1251 74 Forward

3864958 -CAC TCA- 1427 1711 95 Reverse

3865022 -CAC TCA- 279 1271 331 Reverse

3865036 GTG TAG 79 492 138 Forward

3865054 -CAC TCA- 302 793 164 Reverse

3865102 -CAC CTA- 27 731 235 Reverse

3865156 -CAC TTA- 416 808 131 Reverse

3865160 GTG TAA 136 375 80 Forward

3865172 -CAC TTA- 731 1123 131 Reverse

3865228 GTG TAA 197 286 30 Forward

3865230 GTG TGA 272 586 105 Forward

3865378 -CAC TTA- 421 807 129 Reverse

3865470 GTG TAG 98 742 215 Forward

3865632 GTG TAA 46 456 137 Forward

3865710 -CAC TCA- 287 448 54 Reverse

EXAMPLES

The examples below are carried out using standard techniques, which are well known and routine to those of skill in the art, except where otherwise described in detail. The examples are illustrative, but do not limit the invention. Example 1 Isolation of DNA coding for a virulence gene in Streptococcus pneumoniae

As mentioned above each of the DNAs disclosed herein by virtue of the fact that it includes an intact open reading frame is useful to a greater or lesser extent as a screen for identifying antimicrobial compounds. A useful approach for selecting the preferred DNA sequences for screen development is evaluation by insertion-duplication mutagenesis. This system disclosed by Morrison et al., J. Bacteriol. 159:870 (1984), is applied as follows.

Briefly, random fragments of Streptococcus pneumoniae, strain 0100993 DNA are generated enzymatically (by restriction endonuclease digestion) or physically (by sonication based shearing) followed by gel fractionation and end repair employing T4 DNA

122

SUBSTTTUTE SHEET (RULE 26) polymerase. It is preferred that the DNA fragments so produced are in the range of 200- 400 base pairs, a size sufficient to ensure homologous recombination and to insure a representative library in E.coli. The fragments are then inserted into appropriately tagged plasmids as described in Hensel et al., Science 269: 400-403(1995). Although a number of plasmids can be used for this purpose, a particularly useful plasmid is pJDC9 described by Pearce et al., Mol. Microbiol. 9: 1037 (1993) which carries the erm gene facilitating erythromycin selection in either E. coli or S. pneumoniae previously modified by incorporation of DNA sequence tags into one of the polylinker cloning sites. The tagged plasmids are introduced into the appropriate 5. pneumoniae strain selected, inter alia, on the basis of serotype and virulence in a murine model of pneumococcal pneumonia.

It is appreciated that a seventeen amino acid competence factor exists (Havastein et al., Proc. Nat'l. Acad. Sci. USA 92:11140-44 (1995)) and may be usefully employed in this protocol to increase the transformation frequencies. A proportion of transformants are analysed to verify homologous integration and as a check on stability. Unwanted levels of reversion are minimized because the duplicated regions will be short (200-400 bp), however if significant reversion rates are encountered they may be modulated by maintaining antibiotic selection during the growth of the transformants in culture and/or during growth in the animal.

The S. pneumoniae transformants are pooled for inoculation into mice, eg., Swiss and/or C57B1/6. Preliminary experiments are conducted to establish the optimum complexity of the pools and level of inoculum. A particularly useful model has been described by Veber et al. (J. Antimicrobiol. Chemother.32:432 (1993) in which 10⁵ cfu inocula sizes are introduced by mouth to the trachea. Strain differences are observed with respect to onset of disease e.g.,3-4 days for Swiss mice and 8-10 days for C57B1/6. Infection yields in the lungs approach 10^ cfu/lung. IP administration is also possible when genes mediating blood stream infection are evaluated. Following optimization of parameters of the infection model, the mutant bank normally comprising several thousand strains is subjected to the virulence test. Mutants with attenuated virulence are identified by hybridization analysis using the labelled tags from the "input" and "recovered" pools as probes as described in Hensel et al., Science 269: 400-403(1995). S. pneumoniae DNA is colony blotted or dot blotted, DNA flanking the integrated plasmid is cloned by plasmid rescue in E. coli (Morrison et al., I. Bacteriol. 159:870 (1984)) and sequenced. Following sequencing, the DNA is compared to the nucleotide sequences given herein and the

123

SUBSTTTUTE SHEET (RULE 26) appropriate ORF is identified and function confirmed for example by knock-out studies. Expression vectors providing the selected protein are prepared and the protein is configured in an appropriate screen for the identification of anti-microbial agents. Alternatively, genomic DNA libraries are probed with restriction fragments flanking the integrated plasmid to isolate full-length cloned virulence genes whose function can be confirmed by "knock-out" studies or other methods, which are then expressed and incorporated into a screen as described above.

124

SUBSTTTUTE SHEET (RULE 26) SEQUENCE LISTING

(1) GENERAL INFORMATION

(i) APPLICANT: SmithKline Beecham Corporation and SmithKline Beecham p .1. c .

(ii) TITLE OF THE INVENTION: Novel Coding Sequences

(iii) NUMBER OF SEQUENCES: 185

(iv) CORRESPONDENCE ADDRESS:

(A ADDRESSEE: SmithKline Beecham Corporation (B STREET: 709 Swedeland Road (C CITY: King of Prussia (D STATE : PA (E COUNTRY: USA (F ZIP: 19046

(v) COMPUTER READABLE FORM:

(A MEDIUM TYPE: Diskette

( B COMPUTER: IBM Compatible

( C OPERATING SYSTEM: DOS

( D SOFTWARE: FastSEQ for Windows Version 2.0

(vi) CURRENT APPLICATION DATA:

(A APPLICATION NUMBER: PCT/US97/19226

( B FILING DATE: 27-OCT-1998

( C CLASSIFICATION:

(vii PRIOR APPLICATION DATA:

(A APPLICATION NUMBER: 60/029,930

( B FILING DATE:

(vii ) ATTORNEY/AGENT INFORMATION:

(A NAME: Gimmi, Edward R

( B REGISTRATION NUMBER: 38,891

( C REFERENCE/DOCKET NUMBER: P50577

(ix) TELECOMMUNICATION INFORMATION:

125

SUBSΠTUTE SHEET (RULE 26) (A) TELEPHONE: 610-270-4478

(B) TELEFAX: 610-270-5090

(C) TELEX:

(2) INFORMATION FOR SEQ ID NO : 1 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 495 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS : single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 1 :

CTCGGTGATA GAAATAGTGT AATCATGCTT TTCTCTTCTT ATCTATACTT TGCTACTTCT 60

ATTATACAAA AAAATAAAGC GCTTGACTAG GGATTTTTAG AAAAAAAGCC TATTTTTTCA 120

AGAAAAATAG GCTTTTTGCG AACGATTGAC ACAATTGGAT TTGGTTAATT CACTCTTAAC 180

GATGGTTTTA AACGATATAT ATTTTTATAT ATGTAAATTA AAAACTTCTT TCCTTTCACT 240

TCCTACGACT TTTCAGATAC AGATAGCCAA AGAAGTTTTC ATAGAGGGCA AAAAAGAGGA 300

GGAAGGCATG AAGAAAGAAG GTCTCTGGCA AAATCATAAT AACAGGATCC TTGGCTGGAT 360

CAAAAAGCCA GGTATCATCT CCCACAAAGA GAATTTGATG GAAAAGAGTA AAGAATTGGT 420

CAAAACCAAT CAAAACTCCC CCAAGTCCAT CATCACAGGT AAGACTACTA GAGCCAGGAG 480

ACTTTTTCGA TAAAG 495

(2) INFORMATION FOR SEQ ID NO : 2 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 529 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 2 :

CTAGAGCAAG TATTTTTCAA ACTTTTTCCG AATAAATAGA TAGAGCCAGA GAATTTAGTA 60

AACCTAGATT TAAAAATGTG CTATAACATA ATATATTGAA TCTATAATAG TACACCTTGA 120

CTGCTAAAAT ATTTCTATAA ATTAATTTGA CTTTCCTGAT AGAGTTATTC ACATCTTATT 180

TCAACTCACT ATAGAAGGAG GAATAGGAGG ATTCTCAGAC ATCCGGGCAT CAGCCCAACT 240

AATGATTTGA TTGCTAAGAA AATATTCAGC AATCCAGAAA TCACTTGTCA ATTTATTCGC 300

GATATGCTGG ACTTGCCAGC AAAAAATGTT GACCATTTTG GAGGGAAGCG ATATTCACGT 360

ATTACTCTCC ATGCCTTACT CAGTGCAGGA TTTTTATACC AGTATAGACG TCTTGGCGGA 420

126

SUBSTTTUTE SHEET (RULE 26) GTTGGATAAC GGTACTCAAG TAATTATTGA GATTCAAGTC CATCATCAGA ATTTTTCATC 480 AATCACTTGT GGACTTACCT GTGCAGTCAG GTTAATCAAA TCTTGAAAA 529

( 2 ) INFORMATION FOR SEQ ID NO : 3 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 885 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 3 :

CTCATCATCT GTCAAAAAGC GTTTCTTAGC AGTCGTGATA TCCATAAAAT AATCTAATAT 60

CACGATTTCC TCATCCGCAA AGAAAGGAAG GCTGACCAAC TCCAGTGCCA CATCCTTGTA 120

AACTACTTCT TGCATATCAA AGTAGGCAAA GTTGAGGTCA GCAGAATCAT ACCCAATCTG 180

TTTCAACACT TGACTCTTCA TCACTTCAAA CTGACCCTGA TCTGTCCCTG TAAATAGGCG 240

CAGGCTCGGT AAATTCGATA AAGTCAACTT CTGACTTTCT TCAATGGCTA GCATCGTCTC 300

TCCTTTCTTC AGATTTTTCG ATTTAATTTA GTCAATATAG CGCAATTTCC CACGGAAATC 360

TTCTAAGCTC TCGTAGCCTT TTTCCACCAT GATTGCTTTC AGTTCATTGG TAAAGCGGTC 420

AAAAGCACTG ACGCCTTCTT TGTGAAGGGT CGTTCCCACC TGCACCATAC TTGCTCCACA 480

GAGGATGTGT TCAAAGGCAT CTCGACCAGT CAGAACGCCA CCTGTTCCGA TAATTTGGAT 540

TTGAGGATTT AAACGTTGAT AAAAGGCGTG AACATTGGCT AGAGCAGTCG GTTTGATGTA 600

TTATCCACCA ATTCCACCAA AACCATTCTT AGGCCGAATA ACGACAGATT CGTCTTCTAT 660

ATAGAGGCCG TTTCCGATAG AGTTAACGCA GTTGACAAAC TTGAGCGGAT ATTTGTTGAA 720

AATAGCTGCC GCTTGATCAA AGTGAACAAT ATCAAAATAA GGTGGCAATT TAATTCCAAG 780

AGGTTTGGTG AAGTAAGCAA ACACTTCTGC CAAAATCCGG TCTGTTGTCT CAAAATCATA 840

GGCAATCTGA GGTTTACCTG GAACATTTGG ACAGGAAAGA TTTAG 885

(2 ) INFORMATION FOR SEQ ID NO : 4 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 925 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 4 :

TCTTGGCCAA CTGCATGGAG TTCAGCGGTC AATTTCAACG CACCTGAGAA ACAGACCCCT 60 GCACCCCTGA AATCTCAGGA GACATGATGG TCTGGATGGA ATCAATAATG AGAAAGTCTG 120

127

SUBSTTTUTE SHEET (RULE 26) GCTGGATACG CTACCACTTC TGCACGAACA CTCTGCATAT TGGTCTCTGC ATAGAGATAA 180

AACTCACTAT CAAAATCACC TAAGCGCTCT GCACGTAGTT TAATCTGCTG GGCAGACTCC 240

TCCCCACTGA CATAGAGAAC TGTCCCCACT TGGGACAACT GGGTTGAGAC TTGTAGGAGA 300

AGAGTTGATT TCCCAATCCC AGGATCCCCA CCGATGAGGA CGAGACTTTC CTGGTACAAC 360

TCCGCCTCCA AGCACACGGT TGAATTCCTC CATCTCCGTC TTGGTTCGAT TGACATTGAT 420

GGAAGTCACC TCAGCTAGTT TCATGGGCTT GGTTTTCTCA CCTGTCAAGG ACACACGCGC 480

ATTCTTGACC TCGGCAACCT CAACCTCTTC CACAAAAGAA GACCAAGACC CACAGTTGGG 540

GCAACGTCCC AGATATTTAG GGGAATTATA CCCACAATTT TGACATACAA ATGTCGCTTT 600

TTTCTTTGCG ATGACAAACC TCTTTCTATA TCTCTAACTC ACACTCAATC ACTTGGCAAA 660

AATCAATCTT CTCATTTGGC ACAAACTGGC GCATGAGCAT TCGATGAGCA ACAACTACCA 720

CAGTCTGATG TTCTCGATAC TTAGACATAC ATTCTAGAAA CCGAGACTTC ATTTCCGTAG 780

CTGTCTCATA TTGAATAGGA CTATTAGGAA GCAACTCCCC CTTGTTTTCT AAAAACAGTC 840

TTCTAGCTGT TTCAAAGTTT TCTATTCCTG TTTTATAGAC CTGCCATTCA TGTAATAAAG 900 GCTCTACTCT TAAAGGAAGA CCCGT 925

(2) INFORMATION FOR SEQ ID NO : 5 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 602 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 5 :

TTATGTCAGT GGGATTACGC CTAATCTCCC AGAAGCAGAA TTATTATCCG GTCAGGAAAT 60

TAAAACCTTG GNAGACATGA AAACTGCAGC GCAGAAATTG CATGATTTAG GAGCGCCAGC 120

AGTCATTATC AAAGGGAGGC AATCGTCTTA GTCAGGACAA GGCTGTGGAT GTCTTTTATG 180

ATGGACAGAC CTTTACTATC CTAGAAAATC CAGTTATCCA AGGCCAAAAT GCTGGTGCAG 240

GTTGTACCTT TGCCTCTAGC ATTGCCAGTC ACTTGGTTAA AGGTGATAAA CTTTTGCCAG 300

CAGTAGAAAG CTCTAAGGCT TTCGTTTATC GTGCTATTGC ACAAGCAGAT CAGTATGGAG 360

TAAGACAATA TGAAGCAAAC AAAAACAACT AAAATCGCCC TTGTATCCCT ATTAACCGCC 420

CTTTCTGTGG TTCTAGGTTA TTTCTTAAAA ATCCCAACAC CTACAGGNAT TCTAACTCTT 480

TTAGATGCTG GTGTCTTCTT TGCGGCCTTT TACTTTGGTA GTCGTGAAGG AGCGGTAGTC 540

GGAGGACTAG CAAGTTTCTT GCTTGACCTC TTATCAGGCT ACCCTCAGTG GATGTTTTTT 600

AG 602

(2) INFORMATION FOR SEQ ID NO : 6 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 456 base pairs

(B) TYPE: nucleic acid

128

SUBSmUTE SHEET (RULE 26) (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 6 :

CTGGATACTA AGAGAAATCA AAAAAGCACT CTAGGATAGA GGCCTAAAGT GCTTAGTTTC 60

AAGGCTTTAC AGCCTATCAT ATTTAATAAA A ATTACAAC ATCTTGTTGT AGAATTCAAC 120

GACAAGTGCT TCGTTGATTT CTGGGTTGAT TTCGTCGCGT TCTGGCAAGC GAGTCAATGA 180

ACCTTCCAAT TTTTCAGCGT CGAATGATAC GAATGCTGGA CGTCCAAGAG TAGCTTCTAC 240

TGCTTCAAGG ATTGCTGGAA CTTTCAATGA TTTTTCACGA ACTGAGATCA CTTGACCTGC 300

AGTTACGCGG TATGATGGGA TATCAACGCG TTTCCCGTCA ACAAGGATGT GACCGCTGGT 360

TTACAAATTG GACCAAACTT GACGACCAGT AGTCGCGAGA CCAAGACGGT AAACAACGTT 420

ATCCAAACGA CGTTCCAAAA GAAGCATAAA GTTGAA 456

(2) INFORMATION FOR SEQ ID NO : 7 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1961 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 7 :

CTAATATAGA ATAATCACCG CCGTTGTGAA AGAACGATTG GATGATAATC CAATCGTTCA 60

GGGAAATTGG AAGACCTTGG GTTTCCAATT TAGGCATGAG ACACCTTTGG TGGCTGCTGC 120

CGTCCCTCAC AAGCTAAGGT GATTGTTGAA AAAGAGGAAA AAGGAGAAGA AATGAAACCA 180

GTAATTTCCA TCATCATGGG CTCAAAATCC GACTGGGCAA CCATGCAAAA AACAGCAGAA 240

GTCCTAGACC GCTTCGGTGT AGCCTACGAA AAGAAAGTTG TTTCCGCACA CCGTACACCA 300

GACCTCATGT TCAAACATGC AGAAGAAGCC CGTAGTCGTG GCATCAAGAT CATCATCGCA 360

GGTGCTGGTG GCGCAGCGCA TTTGCCAGGC ATGGTAGCTG CCAAAACAAC CCTTCCAGTC 420

ATTGGTGTGC CAGTCAAGTC TCGTGCTCTT AGTGGAGTGG ATTCACTCTA TTCTATCGTT 480

CAGATGCCGG GTGGGGTGCC TGTTGCGACC ATGGCTATCG GTGAACTCTT TTTTAGGATA 540

TAAAACAGGG TTCGGATAAG TTTTTTTGCA AGGTGGATGA TGGCTACATT GTAATGTTTT 600

CCTTGTTCTA ACTTAGTCTT AAAAGCAGGT GAAAAGTGAG GGCATGCTTT GGCAGCTTGT 660

ATGAGTACCT ACCGCAGATA AGGGGAACCC CGTTTGACCA TCCTCCCAGC TAAATCAATC 720

TGACCTGACT GATAAATAGA AGAATCCAGT CCAGCGAAAG CTTGTAATTG AGCAGGATTA 780

TCAAAGGCAT GAATATTTCG AATCTCGGCT AAAATGACCG CCCCTAAACG ATTCTCAATC 840

CCAGTAACCG TCGTGATGAC CGAGTTTAAC TCAGCCATCA AGTCATTGAC ACATTTTTCC 900

GCCTTGTCAA TGAGCCTCTT GTAATGTTTG ATGTTTTCAT TACACGAGAT AAAACGTCTA 960

TGCGTTATCA AACTCATTAC CAATTAAAAC AAATGTGGTT AGATCCTTTC GGAAATTGTC 1020

129

SUBSTTTUTE SHEET (RULE 26) AAGCGATTGG AGGAAATGAA CTAATCCACA GCGGCTTATT CCAAGTATAC CACTTGGGCT 1080

TTGGCAGTAG CTAACTGCGC TAAATATAAT ATAAGGAGGA GTAAAATGAA GACAGTTCAA 1140

TTTTTTTGGC ATTATTTTAA GGTCTACAAG TTCTCATTTG TAGTTGTCAT CCTGATGATT 1200

GTTCTGGCGA CTTTTGCCCA AGCCCTCTTT CCAGTCTTTT CTGGACAAGC GGTGACGCAG 1260

CTAGCCAATT TAGTTCAAGC TTATCAAAAT GGGCAATCCA GAACTTGTAT GGCAAAGCCT 1320

ATCAGGAATT CATGGTCAAT CTTGGCCTGC TGGTTTTGGG TTCTATTTAT CTCTAGGTGT 1380

AATATAAACA TGTGTCTCAT GACGCGCGTG ATTGCAGAAT CGACCAACGA GATGCGCAAA 1440

GGTCTCTTTG GTAAGCTTGC TCAGTTGACG GTTTCTTTCT TTGACCGTCG ACAAGATGGC 1500

GATATCCTGT CTCATTTTAC CAGTGATTTG GATAATATCC TCCAAGCCTT TAACGAAAGC 1560

TTGATTCAGG TCATGAGCAA TATTGTTTTA TACATTGGTC TGATTCTTGT CATGTTTTCG 1620

AGAAATGTGA CGCTGGCTCT CATCACCATT GCCAGCACCC CATTGGCTTT CCTTATGCTG 1680

ATTTTCATCG TGAAAATGGC ACGTAAATAC ACCAACCTCC AGCAGAAAGA GGTAGGGAAG 1740

CTCAACGCCT ATATGGATGA GAGCATCTCA GGCCAAAAAG CCGTGATTGT GCTAGGAATT 1800

CAAGAGGATA TGATGGCAGG ATTTCTTGAA CAAAATGAGC GCGTGCGCAA GGCAACCTTT 1860

AAAGGAAGAA TGTTCTCAGG AATTCTTTTC CCTGTCATGA ATGGGATGAG CCTGATTAAT 1920

ACAGCCATCG TCATCTTTGC TGGTTCGGCT GTACTTTTGA A 1961

(2) INFORMATION FOR SEQ ID NO : 8 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 375 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 8 :

CTATCTCCAA GTNCGNTTGG AATNCCTCCG CNANCCACAA CTCATCCAAG CACTTTNCAA 60

CGTGNCCTGG TCCGGTCCTC CAGTGCGTCT NACNGCACCT TCAACCTGCN CATGGGTAGG 120

TCACATGGCT TCGGGTCTAC GTCATGATAC TAAGGCGCCC TATTCAGACT CGGNTNCCCT 180

AGGGCTCCGT CTCTTCAACT TAACCACGCA ACAGAACGTN ACCCGCCGGT TCATTCTACA 240

AAAGGCAGNC TCTCACCCAT TAACGGGCTC GAACTTGTTG TAGGCACACN GCTTCAGGTN 300

CTATTTCACC CCCCTCCCGG GGAGCANCTC AACTGACCCN CACGGCACCG GTGNANNAAA 360

CGGTCACTTA GGGAG 375

(2) INFORMATION FOR SEQ ID NO : 9 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 665 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

130

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 9 :

GGGGGGGGTN NNTTCTGGGG CCGGGTGNNT CCTNGAAAAA ATGCTGGACT TAACGGTTAA 60

ATCATTTGAA TTGGCCTGTG GATTTTAGCT AGCAATCCAG AGCGAGTTTT CTCCAAGACA 120

GACCTCTATG AAAAGATCTG GAAAGAANAC TACGTGGATG ACACCAATAC CTTGAATGTG 180

CATATCCATG CTCTTCGACA GGAGCTGGCA AAATATAGTA GTGACCAAAC GCCCACTATT 240

AAGACAGTTT GGGGGTTGGG ATATAAGATA GAGAAACCGA GAGGACAAAC ATGAAACTAA 300

AAAGTTATAT TTTGGTTGGA TATATTATTT CAACCCTCTT AACCATTTTG GTTGTTTTTT 360

GGGCTGTTCA AAAAATGCTG ATTGCGAAAG GCGAGATTTA CTTTTTGCTT GGGATGACCA 420

TCGTTGCCAG CCTTGTCGGT GCTGGGATTA GTCTCTTTCT CCTATTGCCA GTCTTTACGT 480

CGTTGGGCAA ACTCAAGGAG CATGCCAAGC GGGTAGCGGC CAAGGATTTC CCTCCAATTT 540

GGANGTTCAA GGTCCCTGTT AAATTTCCCC CATTTAGGGG CAACCTTTTA ATGAAANTTT 600

CCNTNATTTG CCGGGTANCT TTGAATCCCT NGGAAAAAAC CCAACNAAAA AAAGGGCTTA 660

NNCCC 665

(2) INFORMATION FOR SEQ ID NO: 10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 989 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:

CTACGATATC TTTGGTCTTT TGTAAGATAT GAGGTCCACC CTTATGCGCC TCAGTTGGCA 60

TTTCATGCGA TTCAAGAAGT TGCCCCTCTT GATCAACCAA ACCATACTTG ATGTTGGTTC 120

CACCGATATC AATTGCAACG TAATATGTCA TAAATACCTC CTTTTAGATT AGAGGAAGCG 180

CTCCTTGGTT TCACGAATCA AGGCAGCAGC CGCTTCTACA ACTGGACGAT CTTCTTCAGT 240

CACTGGTGTC AATGGTGAAC GAACAGATCC AATATTCAAG CCTTCATTGA TTTTCAAGAC 300

TTCTTTGATG ACACCGTACA TATTTCCATG AGCAGAAGTG AGTTTACCAA TGATTGCGTT 360

GATAGCATAC TGCAATTCAC GCGCTGTTTC TAGGTCCTTA TCCGCAATCA ACTGATTGAG 420

TTTCAAGAAG AGTTCTGGCA TAGCACCATA AGTACCACCG ATACCAGCCC TAGCCCCCAT 480

GAGGCGTCCT CCTAGGAACT GCTCATCAGG ACCATTAAAG ACGATATGGT CTTCTCCACC 540

AAGGCTGACA AAGGTTTGGA TATCTTGAAC TGGCATAGAA GAGTTCTTCA CACCGATAAC 600

ACGAGGATTT TTCAACATTT CTGTGTAAAG GCTTGGAGTC AAAGCAACCC CTGCCAATTG 660

AGGAATGTTG TAAATCACGT AGTCTGTGTT TGGAGCTGCA GAACTGATAT CGTTCCAGTA 720

TTTGGCAACT GAGTTATTCT GGCAAGCGGA AATAAATTGG TGGAATCCGT TGCAATAGCA 780

TCTACTCCCA AGCTTTCAGC ATGGCGAGCA AGTTCCATAC TATCTTTAGT ATTATTGCAA 840

131

SUBSTTTUTE SHEET (RULE 26) GCAACATGGG CAATAATGGT CAATTTACCT TTGGCTACCG CCATGACTTC TTCCAAAATC 900 AACTTGCGAT CTTCAACGCT TTGGTAGATA CATTCACCAG AAGAACCATT GACATAAGAC 960 CTTGAACACC TTTATCAATG AAGTATTGA 989

(2) INFORMATION FOR SEQ ID NO: 11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1450 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:

CTCCATATTT CTTAGCCTTC TCAATTAGGG TCTTGAAGTC TTCGACACCA CCGATACGCT 60

TACCAATATC AGCATAGTTC AAGTGACCAG AGTCATGGCT GTGATATCCT TAACTTTTTC 120

CCAACCTTGA GGGTTGTTCA TAATGCTACG ATAAGCAATG GCACCATCTT GCCAATCAAC 180

TTTCTTGTCT GCATTGGCAT CTTCAGTGAT AACAACCTTA GCACTTGGAA GTTCCTTCGT 240

GTATTCTGGG AAAACAATGC CCTTATAAGC TTTTTCCCAT TGCCATTCAG AGCTGTGGAT 300

TCCTACATAG TTGGCATTTC CGACTGTTTC TTTATAAGCT GTCAAACGAG TCCAGTCATT 360

CGAACCACCA CCATAGCTAT TTTGAGAGTT ACTCCAAACA CCAGCAGCAA GCTTATCTGT 420

AGAAACAAAT CCATACATGT AACCCTTAGC CAAATCCTTC ATTGGATTGG TTACATCGAT 480

ATGATCATCT CCGCTGACAT GCGTATTGTT TGACATGGTT GCCCCATCAA ACTTAGCACC 540

AGTTTGATCA CTAGAAACAG AGACTAAAGC ATTGCCGAGG AAACTAATAG AAGAAAGTAG 600

TTTTCTTTCG TCATCAATCT TTTGACCTGG AGTGACTTGA TTGTGGTTGA CAATCTTGGT 660

CACATCAAAG TGCAATTGAT TGTCCACAAC TTGCAAGCGT ACTGTCATTT CCGCATTGAT 720

TAAGTGAGCA TCATCGCGAA GCTTCATCAA GTACTCTGCT GTTGTCTCAT TGATTTTTTT 780

ATAAGTGACT TCAGGGGTGA TTCGGTGGTT ATTGATAAAG ACTTGGTTGA ATTGTTGCAC 840

CTGTCCTGGC AAAGTATGTC CATTCAAGGT GTATCCCTTG ACACGAAGGA AGGCTTGGTC 900

AATTACTGCC TTAAGTACCT TAAACTGGAT CGTATCATAA GTCACCTTGC TATCGTCAAC 960

AACCGGACCT GTTTCTTTCT GGGCAGGGGT ATCCTCTGGG TTTTACCCTC TCTGTGGCTA 1020

TCCGTTTCAA CGCTTGAACA ACTGGTCGCT CATCGTCATA AGAGCCCGCC TTGAGAAAAA 1080

TCTTCTTCTC ATTTCTAAGA TGGTCATTGA CCGCAGCTGG TAGAGTCACT GTGTCAAAGA 1140

AGATTGACAT CCTTATTTGC CTGGCATTTA CCTGACCGTC TGACTTGAAG ACTGATAGAG 1200

AGACGGTTTG TTGATCCTGT TTCAGGAGCA GCAACACGAC TACCTCTATA CCAAGTGCTA 1260

GTTGTTGGAG ATTTATACTC CCAGAACCAG CCATCCTTGT CATAACCGAC AAAAACATTA 1320

TTATTGGTAT CTTTAAATTT CAAGGAGACA CCAAAGCGTG ATTTGCCCTT TTCAGAATCT 1380

TCTTTGAAGG TTAAATCAAC AGTTGCATTT CCATTGGCAT CAACGGTCAA GCCCTTCTTT 1440

TCAAACAGAG 1450

(2) INFORMATION FOR SEQ ID NO: 12:

132

SUBSTTTUTΈ SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 420 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:

CTGCGAGTTG TGAGGCTCCT ATTATGTCTC GTGATTAAAA TCTCTATAAG GTGATTTTGG 60

AGGGAAATTA TCGGGCGACA GCGGGTAGAG AAGAGATGAA AGAGGCTATT TTGGAATATC 120

AAGCAAATCC TGCTGCCTTA AAAGATCTCA AAGAAAAGGC TAAGAATATT TCCAGAGAGT 180

ATTCTGAAGA GCATCTGTTA CAAATCTGGT TGGACTTTTA TGAGAAACAA GCCGCTTTAG 240

GGACAAAGTA AAAAGTGAGG TAATCTATGC GAATTGGTTT ATTTACAGAT ACCTATTTTC 300

CTCAGGTTTC TGGTGTTGCG ACCAATATCC CAACCTTGAA AACCCACCTT GAAAACACGG 360

ACTTGCCTGC ATTTNTATCT CATACAATCC ACCGAATTTC GATGTCCCCC TCCCTACAAC 420

(2) INFORMATION FOR SEQ ID NO: 13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 661 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:

CTCCCCAAAC TTTTATTTGA GAGTGAACGG TATAAGAATA TGAAACCGGA GGTTAAGGTG 60

GTTTACTCAG TTTTAAAAGA TCGGTTGGAG TTGTCTTTGA GCAAAGGTTG GATTGATGAG 120

GATGGGACTA TTTATTTGAT TTATTCCAAT TCAAATTTGA TGGCACTTTT AGGCTGTTCA 180

AAGTCAAAAT TACTCTCCAT GTGAGTTTGA AGTGACATTT TTAGATGATT ACCATAAAAA 240

ACATAACTAC CCACTATTTT ACGAATCCTA TCTTCAAAAC GTTATGGAAT TCCTTGAAAG 300

TCAAGACATA AAGAATGGGG TTGATGCCTT TGTAGATGAT CATCAAAATC TCGTTTTTGT 360

TTTATATGGA CAAGGCTATC GAGCCGAGGG AAAAGAGGGA ATACTTACAA CCCAAGTAAC 420

TGTAAAAGCT TATGATGAAG ACAAGAAACC GATTAACTTC GCAAATTTAT TAGATTCCTT 480

AATCGTGTCA GAATATCAAA TGGAACCGAA TCTTTGGGAG GTCTCCTATG ATTGATCTCT 540

ATCTAAGTAA AAATAGCCGA AGAAATCAAC TTCTTTTAGA CTTCTTCCAA AACTATGGCA 600

TCGAGGTATC TTGTCATTCA GTTTCTGAAA TGACAAAGGA CAAATTAATT GAGATGATGA 660

G 661

(2) INFORMATION FOR SEQ ID NO: 14:

133

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1429 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:

CTGCCCCTGT AAGGCTGGAC GATTGCCTTT CTTAGTATCC GCAAAGAGGT AAACTGAGAA 60

TAGAGAGGAT TTCTCCTTCA ATATCTTTGA CAGACAGGTT CATCTTGCCT TCTACGTCTG 120

AAAAAATCCG CATATTGACC AGTTTTCTCA CAGCATAGTC CAAATCTTCC TCTTGGTCCT 180

CTGGTCCAAC ACCAACCAGC AATAAAAGTC CCTGATTGAT TTTTCCCTGA ATCTGGCCTT 240

CTATACTCAC TTGGGCTTTT TTAACCCGTT GGATAATGAT TTTCATAATA GCCTTTCTAG 300

TAAGAGCTAG GACAACTAGC CGTTGGTCCG TTTGACAGAG TAAACTTCTG GCACACTCTT 360

AATTTTATCG ACAACCGTGG TCAGTGTAGA GAGGTTGGCA ATACCGAAGG ACACATGGAT 420

ATTAGCAAAC TTCATATCCT TGGTTGGTTG GGCATTGACC GTTGAAATAT TCTTGGTTGT 480

ATTTGAAAGA ACTTGCAGTA CATCGTTCAA CAGTCCTGTA CGGTTGAGAC CGTAGATATC 540

GATATGGGCC ATATACTCCT TATTTGAGCT AGAGTACTGG TCTTCCCATT CCACATCAAG 600

GAGACGTTGC TCGTAGTTTT CTTGGGCACG CAGGTTCATA CAGTCCACAC GGTGAATAGC 660

CACACCACGA CCCTTGGTAA TGTAGCCAAC AATATCGTCA CCAGGCACGG GGTTACAACA 720

CTTAGCAATC CGCACTAGGA GACCAGAAGC ACCTTCAATA ACCACTCCCC CCTCATGCTT 780

GACCTTGGAG AGTTTCTTTA TTTTCAACCT TGACCTCGCC ACCTTTGACA AGCTCCTCTG 840

CCTCAGCCTT GGCCTTGGCA CGCTCTTCCT CACGGCGTTC TTTTTCAGTC AGACGGTTAA 900

AGACGGTAAT CGCACCGATT TCCCCAAAAC CAATGGCCGC AAAGAGGGAG TCTTCTGTCT 960

TGTAACTGGT CTTTTGCAGA ACTTGATCCA TGTGGCGCTT GTCCATAAAT TTATTTGCCA 1020

CATAGCCATT TTCTTGGAAC TGAGCCATCA GCATCTCACG ACCCTTGTTG AC GACAATT 1080

CCTTATCTTG GTTTTTAAAG AACTGGCGAA TCTTATTGCG CGCCTTGCTA GTCTTGACCA 1140

TATTGAGCCA GTCACGGCTA GGTCCAAAGG AGTTCGGGTT GGCGATAATT TCAACCTGAT 1200

CCCCTGTCTT TAACTTGGTT GTCAGTGGAA CCATGCGGCC ATTGACCTTG GCACCAGTTG 1260

CTTTTTCACC GACCTTGGTA TGGATTTCGT AGGCAAAATC AATCGGTCCT GAATCTTTGG 1320

GAAGAGAACG GACAGCTCCA TCTGGGGTAA AAACGTAAAT CTCCTCAGCC AGATAGTTTT 1380

CCTTAACAGA GTCCACAAAT TCCTTAGCAT CATCAGCCTG GTCTTGGAG 1429

(2) INFORMATION FOR SEQ ID NO: 15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1513 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

134

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:

CTCTGCAATG ATGTACTCAA ACATCTCCGC TTCTAGTTCC TCCTTAGGCA GAGGCAATTT 60

CCCACGTCGC ATCCGGTTCA TAAAGACCGT ATGGTTTTCT AAAATCAAAC TATACAAACT 120

CATGTGGGGA ATATCCAATC CAATGGCTTT AGCCACATTT TCCTTTACTT GCTCCATGGT 180

CTGACCAGGC AGAGCATAAA TCAAATCAAT GGAGATGTTG TCAAAACCAG CCAGTTTCAG 240

GCGATCGATA TTTTCATAAA TATCCTTCTC CAAATGACTG CGCCCAATCT TTTTCAACAT 300

CTTATCATCA AAGGTCTGGA CACCTAGCGA AACACGATTG ACAGCCGAAT TTTTCAAAAC 360

AGCTATCTTA TCCGCATCCA AATCGCCTGG ATTGGCTTCA ATGGTCAACT CTTCCAAGAC 420

AGACAAATCC AAGTTTTTAG TCAAGCCATT CAGTAACACC TCCAGTTGCG GAGCCGACAG 480

GGCTGTCGGT GTTCCACCAC CGATATAAAG GGTTGACAAC TTTTCAATAT CATAAGAACG 540

AAACTCTTCC AGCAGATGCT CTAAATAGCT GTCGACTGGC TGATTTTTGA TGAAGACCTT 600

TGAAAAATCA CAATAATAAC AAATCTGGGT ACAAAATGGG ATGTGCACAT AGGCTGACGT 660

TGGTTTTTTC TGCATAGTAA TTATTATACC ACAAAGACTA GATTCCAGAT AAAAATCACC 720

ATCCCCAGAT ACATAGTCCG TCCGGAGATG GTGATGGTTT ATTCTTCTGT TATATCAATC 780

ACAATCTCTT CTGAGTCATC AAGAGCTTCG GCTTTTTCTT GCCATTGTTC CTTGAGATTA 840

TTTAATTGAT TTTTTGATGC TTCTGTCGCT TGAAAAGCAT AGGATTTAGC TTGAGCAAGT 900

ATACTGTCCA CAGTGATTTC ACCTGACTCA ACCTGTTCTT TTGTTTTCAG AACAAAATCT 960

GTAGCCTGCT CCTTAACTTC TGTCAGTTTT TCACAGACTT GCTCCTTGGC ATACTCCGGA 1020

TCTTCTCTCA AATCATCTAA AAAATCTTGA GCCTGACTGC AAACTTGTTT GCCCTTATCA 1080

CTTGTTAAAA ACAAGGCAAG AGCTGCACCT GAAACGGTTC CTAAAAGGAT TGAGGATAAT 1140

TTACCCATAA GGATTCTCCT TTTTTATTTT TTGAAAAATT TACTTGCAAG ACGAAGAGCT 1200

GACAGACTTG CACCAGTCTT GAGTGTTTTT GAACCAGCTG ATGAAGCTTT CTTGCTCAAG 1260

ACACGCGCAT GGTCATTGAG GTCTGAAACA GATAGAGATA AATCTGCAAC AGCACTGAAG 1320

AGTGGATCAA TCGTAGCCAC CTTGACATTG ATATCATCTG CCAAGACATT GACCTTAGCC 1380

AACAACTCAT TGGTGTGATG CAAGGTCACA TCCACATCTG AAGTCAAGGT TTTAATCGTC 1440

TTTTCTGTTT CATCGATGAC ACGACCAAGC TTTTGTACAG TAATGATCAG ATAGACCAAA 1500

AAGACAATCA CAG 1513

(2) INFORMATION FOR SEQ ID NO: 16:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 505 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:

CTCTTGTCAG AGAAATTTAC AAAACGTTAG GAGAATAAGA TGGCATTTAT TGAAAAAGGT 60 CAAGAAATCG ATATGGAAGT CATCAAGGCT GAAACCCAAT TGTCTGCAGA AGCCTTGAGA 120 CTCAAGGAAA GCCGTGACAG GGAATTGGCA GATATTATTT CAGGGGAAGA TGACCGTATT 180 CTCTTGGCTG ATTGGTCCTT GCTCTTCTGA TAATGAAGAG GCGGTCTTGG AATATGCTCG 240

CCGTTTATCC GCCTTGCAAA AGAAGGTAGC GGATAAGATT TTCATGGTCA TGCGCGTGTA 300

TACTGCTAAG CCTCGTACCA ATGGAGACGG CTATAAAGGG TTGGTTCACC AGCCAGATAC 360

TTCTAAGGCT CCAACCCTGA TTAACGGCTT GCAGGCTGTG CGCCAGTTGC ACTACCGCGT 420

TGATTACAGA GACTGGTTTG ACAACGGCAG ATGAGATGCT TTATCCGTCA AATCTGATCT 480

TGGTGGATGA CTTTGGTCAC CTACC 505

(2) INFORMATION FOR SEQ ID NO: 17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1827 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:

CTCTTTTAAC CGTTTTAGCG GTGACACCGA GGATTTTTTC AGGACCCAAG ACTTGTCGGG 60

CAACCGAAAC TGGGAGTTCG TCATCTCCAA TATGCAGACC AGCAGCATCA ACCGCAAGAC 120

AAACATCCAA CCGATCATCG ATTATCAAGG GGACCTGATA GGCATCTGTT ATTTCCTTGA 180

CTTGTTTTGC CAGTTGATAA TATTGATTGG TTGTGAGATT TTTTTCTCGC AATTGGACTA 240

TGGTAACCCC TGAACGGCAG GCCGTCTCAA CTTTTGCAAG AAAGCTTTCC ACGGAATCTT 300

GATAGCGATT GGTTACCAGA TATAGTCTAA GCGCTTCTCT ATTCATAAAC CTCTCCTTTG 360

ATGGTATCTA GCCAATTTTC ATCTCTTCTT AGGAGCGAAA GCTGATTGAG TACTTGGTAA 420

CGAAATTCTT CCAATCCCAT TCCTTGAACA ACTATTTTCT CAGCAGCGAT ATTGAGATAA 480

GAGACTGCTA AGCAAGAACT TCAAAACCAG TCTTTCCTTG GCTGAGAAAA ACAGCTGTTA 540

AGGCTCCAAC CAAGTCTCCT GTCCCTGTTA TCCAGTCTAA TTCAGTACAG CCATTCTCAA 600

GTACAGCAAC TTGATTCTCC GAAACAATAA GGTCCTTGGG ACCTGTGACT AAGAATGACA 660

TACCACGATA GGTCTGACAC CAGTCTTTCA AGACTTGAAG CAAATCCTCC GTTTCTTGAT 720

CTTTAGCACT CGCATCGACC CCAACGCCGT GATGCTTTAA TCCAACAAGA CTTCGAATTT 780

CTGACATGTT TCCTTTAAGG ACCGTAGGTC TATAGTCTAA AAGGTCTTTA ACTAAGCTCT 840

TACGAATGGA TGAAGTCGTT ACGCCAACCG CATCTACTAC CATCGGGAGA GAAGATTGGT 900

TTGCATACAA AGCTGCCATG CGGATTGCTT TTTCCTTCTC AGCTGACAAA TGCCCCAAAT 960

TGATGAAGAG AGCCTGGCTT TGCTTAGTAA AATCAAGAAC TTCACGGGGA TCATCTGCCA 1020

TGACAGGTTT GCATCCCAGA GCCAAAATCC CATTTGCCAG CATCTCACAA GAAATCTCAT 1080

TGGTCATACA GTGAATGAGG GAACTAGAGC CTATAGGAAA AGGATTTGTC AATGCCTGCA 1140

TCATTCTATC CTTTCAGCAA AGAAATATCC TTGCACTTTT TTAAAGAATT CCTGCTTGAT 1200

TAAAAATCTA AATGCAATAA AGGAAATCGC TGTACCAATC AAGGTTGCTC CGAAAAATCG 1260

AGGCGTGTAG ATAAACCAAC TAAGCTTAGC AGCCGATCCT GTAAAGAGCA CCATAACAGG 1320

ATAGGAAACA ATAGAACCAA TAATACCTGT TCCCACAATT TCTCCCAAGG CAGAAAAGTA 1380

AAATTTTCGA CCGTACTTAT AAAAGAGACC TGCTAGAAGG GCTCCAAAAG TCGCTCCTGT 1440

GAGAGATAAA GGAGCTTATC GGAATACCCT TGAGTCGTCA TACGGATAAA GGCTGTCACT 1500

136

SUBSTTTUTE SHEET (RULE 26) GTAGCCATAG CCAAGGCATA AACAGGTCCC ATCATGATTC CCGCTAGAAT ATTGACTACA 1560

CTGGACATCG GTGCCATTCC CTCAATCCGA AAGATAGGTG TAAGGACTAC ATCAAGGGCA 1620

ATCATCATAG ATAAAATGGT CAATTTGTGA ACTTGTAGTT GGTGCTTTCT CAAGTTTCTA 1680

TTCTTCTCCT TTTTCTAAAG ACTGTAAATC GCTCTTCCAT GTCTGGTGTT GGTAAGCCAT 1740

CTCCCAAAAC TTGGCTTCCA TATGAACACT GATGTGGAAG GCATCTAGCA TTTTTTGCTT 1800

ATCTGTCTCA TCACTTTCTC GATAGAG 1827

(2) INFORMATION FOR SEQ ID NO: 18:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 485 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18:

CTATTGCCAA TCCATATAGC CTATCAGGTG GTCAATAACA ACGTGTGGCC ATCGCTCGTG 60

GCCTATCAAT GAATCCAGAC ATCATGCTCT TCGATGAACC AAATTCTGCC CTTGACCCTG 120

AGATGGTTGG AGAAGTAATT AACGTTATGA AGGAATTGGC TGAGCAAGGC ATGACCATGA 180

TTATCGTAAC CCATGAGATG GGATTTGCCC GCCAGGTTGC CAACCGCGTT ATCTTTACTG 240

CAGATGGCGA GTTCCTTGAA GACGGAACAC CTGACCAAAT CTTTGATAAC CCACAACACC 300

CTCGTCTGAA AGAGTTCTTA GATAAGGTCT TAAACGTCTA AACTCAAACT GCAAGGATTT 360

CCTTGCAGTT TTTCTACCTC GTATTGGAAT TTTTGATTTT TCGGAAAATT ATGTTAGAAT 420

TAAGTTTATG AAATGAGGTT TCCTCATACC TAGCAAGACT AGGAATAAAA ATAGAAATTA 480

GGTAG 485

(2) INFORMATION FOR SEQ ID NO: 19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1547 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:

NTCTTGGGCN CNGGGCGNNT CCTTTGAGGA CNACGGTATC GATGACCTTG ATCTCAAGTG 60

CAAGCAGTAT CTGAATCTGC AGCAGCACCT GTCCGTGCAA AAGTTCGTCC AACATACAGT 120

ACAAACGCTT CAAGTTATCC AATTGGAGAA TGTACATGGG GAGTAAAAAC ATTGGCACCT 180

TGGGCTGGAG ACTACTGGGG TAATGGAGCA CAGTGGGCTA CAAGTGCAGC AGCAGCAGGT 240

137

SUBSTTTUTE SHEET (RULE 26) TTCCGTACAG GTTCAACACC TCAAGTTGGA GCAATTGCAT GTTGGAATGA TGGTGGATAT 300

GGTCACGTAG CGGTTGTTAC AGCTGTTGAA TCAACAACAC GTATCCAAGT ATCAGAATCA 360

AATTATGCAG GTAATCGTAC AATTGGAAAT CACCGTGGAT GGTTCAATCC AACAACAACT 420

TCTGAAGGTT TTGTTACATA TATTTATGCA GATTAATTTA CAGAGGGACT CGAATAGAGC 480

CCTCTTTTCA GGTTTTACCG TGACAATCCC TATTAAAAAT TATATCAAAA TCGTGAAAAT 540

ATTGGAAAAG TATGGTAGAA TGAAAATTGT CGTGTGAACG ATAATACTCA TTCTTGATGA 600

ATTGTGAAGC AGTTGCCCTT GGGTCGTTTT GCGAGTTGAA GTCAAGAAGA GGAAAAAAAC 660

AAAAAGGAGA AATACTCATC GAATTTCAAT GAAACAACTT CTTGAGGCTG GTGTACACTT 720

TGGTCACCAA ACTCGTCGCT GGAATCCTAA GATGGCTAAG TACATCTTTA CTGAACGTAA 780

CGGAATCCAC GTTATCGACT TGCAACAAAC TGTAAAATAC GCTGACCAAG CATACGACTT 840

CATGCGTGAT GCAGCAGCTA ACGATGCAGT TGTATTGTTC GTTGGTACTA AGAAACAAGC 900

AGCTGATGCA GTTGCTGAAG AAGCAGTACG TTCAGGTCAA TACTTCATCA ACCACCGTTG 960

GTTGGGTGGA ACTCTTACAA ACTGGGGAAC AATCCAAAAA CGTATCGCTC GTTTGAAAGA 1020

AATTAAACGT ATGGAAGAAG ATGGAACTTT CGAAGTTCTT CCTAAGAAAG AAGTTGCACT 1080

TCTTAACAAA CAACGTGCGC GTCTTGAAAA ATTCTTGGGC GGTATCGAAG ATATGCCTCG 1140

TATCCCAGAT GTGATGTACG TAGTTGACCC ACA AAAGAG CAAATCGCTG TTAAAGAAGC 1200

TAAAAAATTG GGAATCCCAG TTGTAGCGAT GGTTGACACC AATACTGATC CAGATGA T 1260

CGATGTAATC ATCCCAGCTA ACGATGACGC TATCCGTGCT GTTAAATTGA TCACAGCTAA 1320

ATTGGCTGAC GCTATTATCG AAGGACGTCA AGGTGAGGAT GCAGTAGCAG TTGAAGCAGA 1380

ATTTGCAGCT CCAGAAACTC AAGCAGATTC AATTGAAGAA ATCGTTGAAG TTGTAGAAGG 1440

TGACAACGCT TAATTTATAC AAATAGTAAT TACCTAGGAG GGCGGGGCTT AGCCCGGCTC 1500

TCCTATTTTC AAAAAATATA GGAGAATTAA AATGGCAGAA ATTACAG 1547

(2) INFORMATION FOR SEQ ID NO: 20:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 740 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:

CTATAAAAAA AAGGGTAACC AGTATGGAGG ATGAATGTCT GGAACTATCT GAGAATCTCG 60

GATTTTGGAA ATCAGACCGA TCATCATGAG ATAAGGAAGG AAAGCACTTG TAAAAAGCAC 120

TGTAACCACG CCAGTCCCCT GTCCCAAGAG GGTGAGGTGG TAGCGTAAAA CCATGCGGAA 180

AAATCCCTTT TTAGTGGTTG AAATTCTCTC CTTGCTGCGA CGTTCTTTTT TGACCTTCTC 240

CTCACTATTA AGCAGGATCA CGTCATAAAA ACGAGGAAGG ACCTTCTTTT TGGTCAGATA 300

AAGCAGGAAG AGAGTTAGTC CTATCCAAGC GAGCAGACCC AATATGGCTT CTATTGAAAA 360

AGGCTCCACT GCTATTTTGT AAAAGATATG AAGAGGATAA AGGAGAAATG GAATGTCTCT 420

AACTTTGTCA ACAATACTTC CAAAAGTCGA CTGAAGAAAG AAGATAAATA TTAAAGGTAT 480

138

SUBSTTTUTE SHEET (RULE 26) GAGAACTCCT ATCCCAATCA TCACATTCGA AAAAATAGAC TGATACTTTC TGAAGACCCT 540

AGTCTGAGCC AAGAAATGTA CTGCCACTAC CGTCACTAAA GTAACAGAGA CAAATAATAA 600

GGTCAAGGAC AGTAGCATCA AAGGCAAACC CAGCCAAAGA GAAGGAGCTA GACTAATATA 660

GAGGGCTAGA AAATAAGCTA GGATTGGTAC AATTCCAGTT AGAGCTGGCA AGAGGACAGA 720

CAGTCCTTTA GCAATTCGAT 740

(2) INFORMATION FOR SEQ ID NO: 21:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2219 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:

ATCGAATTCG TTTTGCAAGT GGCGAAATGC GAACCACGTT TGTGTCTTTA TAAGTTTCCA 60

CGTCTTCTTT GTGGACACGA CCGTTTGCAC CTGAGCCAGA AACGTCGTAG AGGTTTATCC 120

CTAAATCATC CGCTAACTTT CTAGCTGCAG GAGTCGCTCT TAGCTTGTCA TCAGCCATGA 180

CCTCTCCAAT TCTATTTATG ATACAAAGGG CGTCAAAAGC GACTGAAAAA TAGGAAATCG 240

ACGATGGCTT CGATGAAGCC AAGGAGATTT ATCTTTTTTT CCAAGCTTTT AGCCCGTGCT 300

CTAATCTAAG ATATTAAGGA CGAAGAGCTC TGCACCTAAA AGATACAAAG TTCTCGTCAG 360

CTTTGTTTTA TTTACATAAC TTATCTTATG TAACTCTATT CTTTGTTATA AGTTTTTCGG 420

ATTGCATCTT TGATACTTTC AACTGTTGGA ATCATTGCAC ATTTTTAGGT TTTGCGCATA 480

AGGCATCGGC ACATCTTCTC CTGCACAACG GCGGATTGGT GCATCTAGAT AGTCAAATGC 540

TTCTGATTCT GAAATAATAG CTGAAATTTC ACCGATATAG CCACTTGTTT TGTGGGCATC 600

GTTGACCAGA ACAACCTTAC CAGTCTTCTT CACTGAGTTT ATGATGATAT CCTTATCAAG 660

CGGAACAAGG GTACGTGGGT CAACAATTTC AACTGAAATT CCTTCTTCAG CTAATTCTTC 720

AGCAGCTTGA ACCACACGGC GAAGCATTTT TCCATAAGTG ACAACTGTTA CATCCGTTCC 780

TTGGCGTTTG ATTTCACCAA CCCCAAGTGG AATTGTGTAG TCTGGATCAA CTGGCACTTC 840

CCCTTTTTGG TTAAATTCTG ACTTGTACTC AAGTATAATA ACTGGGTTGT TATCACGGAT 900

AGAAGACTTA AGCAGGCCTT TCATGTCCGC AGGTGTTCCA GGTGCCACAA CCTTAAGCCC 960

TGGAATGTGA GTAAACCAAG ACTCTAGAGA TTGTGAGTGC TGGGCGGCAG AGCCAACTCC 1020

GTTACCAGCT GCACAACGAA CAGTCATTGG AACCTGACCT TTACCACCAA ACATGTAACG 1080

TGTTTTAGCA GCTTGGTTGA CGATATTGTC CATGGCAATA ACAGAGAAGT CCATGAAGGT 1140

CATATCGACG ATTGGACGAA GTCCTGTCAT GGCTGCTCCT GCTGCAGCTC CAGAGATGGC 1200

AGCTTCAGAA ATCGGACAGT CACGGACACG TTCTGGACCA AATTCTTCAA GCATTCCAAC 1260

AGAAGTACCG AAGTCTCCTC CGAAGACACC GACGTCTTCT CCCATCAAGA ACACATTTTC 1320

ATCGCGAACG CATTTCCTCA GACATAGCAA GGATAATGGT GTCACGGAAG GACATTGTTT 1380

TTGTTTCCAT TTTATCTCTT TCTCCTTAGT CTGCGTAAAT ATCTTCAAAG GCTGATTCAA 1440

GCGGTGGGAA TGGGCTTTCC TCTGCAAATT TAACAGAAGC TTCTACTGCT TCCTTTACTT 1500

GCGCTTGGAT TTCTTCCAAT TCTTCGGCAC TTGCAATGTT ATTTTCAATA AGGTAATTGC 1560

139

SUBSnTUTE SHEET (RULE 26) GGAGGTTTTC GATTGGATCT TTTTGTTTCC ACAATTCCAC TTCTTCACGC GTACGATATT 1620

TACCAGGGTC AGATGATGAG TGACCGAGCC AGCGATAAGT TACACTTTCA ATCAAGACTG 1680

GACCATTGCC ACTGCGAACA TGGTCTATAG CTTTCTGAAA TCCTTCATAG ACATCGATGA 1740

CATTGTTACC GTCTTCGATG AACATTCCAG GAATTCCATA AGCGGCGCTA CGTTGATGGA 1800

TATGTTCTAT ATTGGTCATT TTCTTGATAT CCGCAGAGAT ACCGTAACCG TTGTTAATGC 1860

AATAGAAAAT GACTGGCAGG TTCCAGATAG AAGCCATGTT CACTGCTTCG TGGAAAACAC 1920

CTTCATTGGT CGCACCATCT CCAAAGAAGC AGACAACGAT TTTACCGGTA TTTTGCATTT 1980

GCTGACTGAG GGCTGCACCG ACAGCGATCC CCATACCACC ACCTACGATA CCATTGGCAC 2040

CAAGGTTCCC AGCATCAAGG TCAGCGATAT GCATAGATCC ACCTTTCCCT TTACAGGTTC 2100

CAGTGTATTT ACCAAGGATT TCAGCCATCA TTCCGTTGAA GTCAATCCCT TTAGCAATAG 2160

CTTGCCCGTG TCCACGGTGG TTTGAGGTAA TCAGATCATC TGGATTGAGA GCTACATAG 2219

(2) INFORMATION FOR SEQ ID NO: 22:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1078 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:

CTAACCCTNG ACGGGGCCGC TATCATCAGT CAAACAGCTA AAAATCTTGT CTGCAAAAGT 60

CTCGATTAAC TGAGCTTTTA CAAAAGCCGT ATTTCCTGGA AT ACTTGGA GATTGATCAT 120

CTTATCCATC AATTCAGCCG ATTCGATATT GTCTTCAGCC AGTTGCAGAC TTTTTACGAT 180

TGATTTTGGC AATTCGTAGA CATAGGTGTT GTCTCTCAAA GGAATTTTGA CAATACCTAA 240

CTCTTTGATA TCTCGGGATA CCGTCGCCTG AGTGGCAGTG ATACCTGCTT CTTTCAAATG 300

TTCTACAATT TCTTCTTGCG TGCCGATTTG ATAATCTGTC ACCAATCTTC TAATTTTTTC 360

AAGTCTCTCT TTTTTATTCA TTTTTAAATT GACTATGCGC CCTCTCTACT GCTTCTTTAA 420

TCTCAGCAAG AATCTGATTG CTTGCTGACT TTTCTTTTTT CAAATACACT AAAAATTCAA 480

TATTTCCATG TCCACCTTGG ATGGGAGAAA AGTCCAAGCC AAGGACTGAA AAACCTGCCT 540

CTACTGCCAT AGCTGTTACA GATTCAAGGA CATTCTGATG AATCTTAGCA TCTCGAATAA 600

TTCCATTTTT CCCAATCTGC TCACGTCCTG CCTCAAACTG AGGTTTGACA AGTGCTACCA 660

CCTGACCTTG ATCAGCCAAG ACACGGTGCA AGGCTGGCAA AATCAGACTA AGGGAAATGA 720

AACTCACATC AATACTGGCA AAGCTCGGCT CCTGCTCGAA ATCAGTCTTT TCAGCATAGC 780

GGAAATTGAA CTGCTCCATG CTGACAACTC GTGGGTCTTG GCGTAATTTC CAAGCCAACT 840

GATTGGTACC AACATCGACT GCAAAGACCA ACTTGGCACT ATTCTGTAGC ATGACATCGG 900

TAAAACCTCC AGTAGAGGCC CCGATATCAA TCGTAGTCGC GCCATCCACC GACAAATCAA 960

AGACCTGCAA GGCCCTTTTC CAGTTTCAAA CCACCACGGC TGACATACTT GAGTTTCTCC 1020

CCCTTGAGTT TTAATTCGGT GTCATCTGGA ATTTCTCTCC TGGCTTGTCA AACCGTTC 1078

(2) INFORMATION FOR SEQ ID NO: 23:

140

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 928 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:

ACTTTCCTGA CCTCTGTTTC CAAATAATCT TCCAAATGGA CAGAGATCTA CCGTTGTTTG 60

CATCGATAGC TGAGGTCTTT TTTAGAAAAT ACCATCACTT TTAGAAAATA TAAACACATT 120

TTTCGGATAA GATTAAGGTT AAAAGCAGCT CGTTTATCCA GGGTCTGATG ATGGTCTTCA 180

CGATAAACCA CATCCAATAA CCAATGCATA CTTTCTGCTG ACCAATGACC TCGAACACTA 240

TGGCAAAAGG TCATCAACAT CAAGCTTAAA GTTAAAGATA AAATAGCGAA CGTCTTGACT 300

TGTAATACCA TCTCTATCAA TAGTATTACG AGTCATTCCA ATTCCACGCA ATTTATGCCA 360

TTTGGGATGG TTTTGACACA ACCACTTAAC ATCAGAAGAC ACCCAGTATT CTCGAACTTC 420

AATCTATCCT CTTTCTATAT TCTAACTGAA AGGACAATTC AATGATTCAT TTAATAATGA 480

TTAGCGCCAT TGCTCTAGCC ATTGGAATTG GTTACCGCAC CAAAATCAAT ATTGGCCTGC 540

TGGCTATTGC TTTTTCTTAC CTCATCGCAA CCACTCTCAT GGGATTAAGT CCCAAAGAAC 600

TTCTTCATTT TTGGCCAACC TCACTCTTTT TTACCATTTT TAGCGTCTCT CTCTTTTATA 660

ACGTTGCAAC AACTAACGGT ACTCTTGATG TTTTGGCTCA ACACATTCTC TACCGCACAC 720

GCACCCACCC TAACGCCCTC TACATGATTT TATACCTGAT GGCAACCCTT TTGTCTGCTT 780

TAGGTGCTGG ATTTTTCACT ACTATGGCCG TTTGCTGTCC TCTAGCGATT ACCCTCTGTC 840

AAAAAGCGGA CAAACACCCT TTGATTGGAG TCAAAGCGTC AATGGGAACT TCAGGAAGGG 900

TAATTTGATA ACCAAAGGAA TAAAATTT 928

(2) INFORMATION FOR SEQ ID NO: 24:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 847 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:

AAAAACGCAC CATATCAAAA ACTAAAAAGT TTGATATCAT GCGTCATGTC TTAAACTAAT 60

TGACTATACT TTCTATTCAA ATGAGCTTTT AACCAATTGA TTGAGCCAAT CCACTCTTAA 120

AACCAAAGGA GCAATTTCTC GGCTTAGCTG ACTCTTCTCG GAATCTGAAC CATGTACAAC 180

ATTTTGGATA ATCTCATTTT CTCCAGCAGC TTTTGCAAAA TCACCTCGAA TAGTGCCTGG 240

TAAAGCTTCT TCTGGACGAG TTGCACCCAT CATGGTCCGC CAAGTTTCGA TTACTTTGGG 300

141

SUBSTTTUTE SHEET (RULE 26) ACCAGAAATG ACACCCACAA GAACTGGACC TGAAGTCATG AATTCACGAA TCGGTGGGTA 360

AAAACTCTGA CCAACCAAGT CCTGATAGTG CTGGTCAATC AACTCTTCTG AAAACCTGTG 420

AACGAAACTC CAATTTTTCG ATTGTAAATC CACGTTGTTC GATGCGCTTT AACACTTCAC 480

CCACTAGCCC TCTTTTTACA CCATCTGGTT TGATGATAAA GAATGTTTGT TCCATACCCG 540

TCTCCTTTGT CAGCTTCTTT CTTTTATTTT ACCACATCTC GTGGAAAAAT GGAGAAAGTT 600

TTCAGAAGAG AGAATGAGAG AACCCTCGGG TTCTCTCATT CTCTCTTATT CTACTGTTTC 660

TTCCACAGTG TCAACGGCAG TATCCACAAC TACTTCTGTT GTTTCTTCAT TTCCTTCTTC 720

CTCTACTGGA GGATTAAGGT ATTCTTCTTC GTTGACAGCA TGTGGTTCAA GGTTACGGTA 780

ACGGGCCATA CCAGTACCAG CTGGGATGAT CTTACCGATG AATAACATTT TCCTTTAAAT 840 TCCAAGG 847

(2) INFORMATION FOR SEQ ID NO: 25:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 578 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:

ACAACCTAAC TACCGNCTAA TTCAGCGCGA ACTTCTGCAG TAGCTGCTTC AACAACTTCA 60

CGACGTGAAA GGATGAAGCG GTTTTCTTTA GCGTTAACTT CTTTGATTTT AGTATCAAAT 120

TCTTGACCTA CAAAACGCTC AGCGTTACGT ACGAAACGAG TATCCAACAT TGAAGCTGGG 180

ATAAATCCAC GAACACCTTC AAATTCTACT GAAAGTCCAC CTTTAACGGC ACGCGTTCCT 240

TTAACAGTAA CAACTTCTTC TTCGCGACCA ACAAGTTTGT CCCATGCTTT GCGAGCTTCA 300

AGGCGTTTTT TAGATGACAA GGTATGTAAC TGTATCAGTA TCTTTACCAA CTACTTGACG 360

AAGTACAAGA ACATCCAATA CTTCTCCTAC TTTAACAAAG TCATTGA AT CTGCATCACG 420

ATCGTTTGTC AATTCGCGAA GAGTCAAGAC ACCCTTCAAC ACCAGTTCCC AGAAGAATGC 480

AACGTTAGCT TGAGTCGCAT CAACTGTCAA TACTTCAGCA CTAACACATC ACCAGTCTCA 540 ACTTGACTNA CGCTATTGAG CANATCTTCA AATTCGAT 578

(2) INFORMATION FOR SEQ ID NO: 26:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 888 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:

142

SUBSTTTUTE SHEET (RULE 26) GTAGTTATAG TAGGGGTCGG ATTGAAATGC CACNGCGCTT CTTGGAGTTT CTGATACCGT 60

TTAAAATAGC GTTGGGCATT CTGGTTGGGA GTCAGAGCCT TATCAAGCGC AATCATGATA 120

GGTTGGTTGG TATAGTAGTT GTCTAGGATA ACCTGGTTCT TGGTCGTTAG GCACCTGGTG 180

GAGGAAGGTT GTCAGCAATT CTCCTTTTTG ACGAAATTCT TCAGCGTTGT CTGTCGCCAG 240

TAACTATTTT TCCTGTTTTT TGAGTTTGTG TCGGTTTTTC TGAAGTTCAT TTTCAACACG 300

ACGAATCAGT TCACTGGCCT GCTGTTTGAC GCGGTCGCGC TCAGCCTTAT CCTTATAGTA 360

GGTGTCCAAC AAATCAGAAA GATTTGCAAA AGGCTCTCCC ACCTGATTTG CAAAAGGAAC 420

TGGACTGAAG GAAGTCTCAG TCAAGCATGG CTTGGTTTCC TGATTGAAAA AATTTCGGAA 480

AGCGGAAAGT TTTTCACTAA CCAGTATCCT TTCCAATTCA TTTGCCGTAT CGCGTCCCAG 540

ACCTTGAAAG AGGCTTTGAA GATTTTTTGC TGTTAGTTCT TGGGTTTGCA GGATTTCAAA 600

GAGCTTTTCA TCCTTGATAG TAAAAGGATT GAGAGATTCT GTACTTGGCG GAGCGATATA 660

GGTCGATCCT GGAAGTAAGG TGCGGTAGCT ATTTTGTGAA AAGCCGACGT GTTTGATAAC 720

TTCGAGGATT TTATGACTGC TTTTATCCGA CCAGTTAGAA TATTACTGTG TTTCCCCATA 780

ATTTCGATAA TCAAGGTAGC CTGGATATGG TCTCCAATCT CGTTTTTATT GGAAACTGTA 840

ATTTCCACAA TACGGTCATT TTCCACTTGC TCAATCGACT CAATCAGG 888

(2) INFORMATION FOR SEQ ID NO: 27:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 513 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:

ATCGAATTTT GTTCTTTCAT AGAGAGCTAC CTGAGTTCTA TTCAAGCTCA GGTAGTACTT 60

TCTTATAAAC TAGACAAACT AACTGTCATT CTACCATCAG ATTACAAGAC ATCATCGTCA 120

CTCACCTTGG AATTCAATGT CGTACCCCAA TGGGTAATTT TACGGTGGGG TTGAGCTAAA 180

ATTGGTCTGT TTTCATAGAT TGTTTGCCAT CTATTCCATA GTAGGCCCGT CTTTTTCTCA 240

ATCTTAACTC GCAGATTTCT CATATTTTCT TTGATTGGGA GGTTGAGGAC AAAACCTGCA 300

GTCTGGTTGC GACCGTTTCC TTCCCAAGAA TGACTACGAA CAACTTGGTT TCCATCTTTA 360

TCTACTGGAA CTTCTTCCCA AGTTATGGAG TAGCGGGCAA TGTAAGCTCC ACTGTGTTGA 420

ATTATCAATG TTTTATCTTT CACAGGGAGT CTGACTGATT GGTTGAACTG GCTTAGAAAC 480

TTGTGTCGCC GTTTCAGCAT TCGTAGCTAT AAA 513

(2) INFORMATION FOR SEQ ID NO: 28:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 214 base pairs

(B) TYPE: nucleic acid

143

SUBSTTTUTE SHEET (RULE 26) (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:

ATCGAATTCT AACATGTGCT TCTCCTTCTA TTGTTCCTAT CTTTAAAATC TACTCCTTCA 60

TGCTCCAAGA GCCAAGCTTT CTTTTCCACT CCTGCAGCAT AACCTGTCAG ACGCTTGCCT 120

GCTCCCAACA CACGATGACA AGGTACTAGG ATAGACCAAG GATTGCGTCC CACTGCTCCA 180

CCAATTGCTT GAGCAGAAGC CACTTGCAGG TCTT 214

(2) INFORMATION FOR SEQ ID NO: 29:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1084 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:

CTCCAGCAAT GGATCCAAGT ATGATGGGCG GGATGATGTA AGCTTTCTAT AGAAAACACC 60

TTATAAAAAA CACGAAAGGA GGGAATGACT AACCCTTCTT TTTATAATAT TCACTTCTAA 120

GATTGATGGT GAGCTCTCCT AACTTATATG ATAAAATAAG ACTAGAGGAA AGGAGAAGAA 180

CATGATCGAT GTACAAGAAA TTCTGTGCAA GATGACCCCC AATCAGAAGA TTAATTATGA 240

CCGTGTCATG CAGAAAATGG TACAAGCATG GGAAAAAAAT GAGTAGCGGC CAACCATTCT 300

CGTGCATGTT TGCTGTGCCC CTTGTAGTAC CTATACACTA GAATATTTGA CCAAGTATGC 360

AGATGTGACC ATCTATTTTG CCAATTCTAA TATCCATCCC AAGGCAGAAT ACCATAAGCG 420

GGTCTATGTC ACCAAGAAAT TTGTTAGTGA TTTTAATGAG CAGACAGGAA ATACGGTTCA 480

GTACCTAGAA GCTCCCTACG AACCCAATTA ATACCGAAAA CTAGTTAGGG GGCTAGAGGA 540

GGAGCCCGAA GGTGGCGACC GTTGCAAGGT TTGTTTTGAC TACCGACTGG ATAAAACAGC 600

GCAAGTGGCT ATGGACTTGG GCTTTGACTA CTTTGGTTCA GCCTTGACCA TCAGTCCTCA 660

TAAGAATTCT CAAACTATCA ATAGCATCGG AATCGATGTG CAAAAAATTT ACACGCCCCA 720

CTATCTTCCC AACGATTTCA AGAAAAATCA AGGCTACAAA CGTTCAGTAG AGATGCGTGA 780

GGAGTATGAT ATCTATCGTC AATGTTATTG TGGCTGCGTC TATGCAGCCC AAGCCCAGAA 840

TATTGACCTG GTTTAAGTTG AGTAGGACGC CACAGCATGC TTGCTGGATA AGGATGTTGA 900

GAAAGACTAT TCTCATATCA CATTTATAGT AGATTGAAAC TAGAATAGTA CACCTTTACT 960

TCTCAAACAT TGTTAGAAAT CGATTCGGCT GTCCTTATTT CATTTTAATA TACTGGTACG 1020

AAATTAGATA TATCAATGAT AACTTGCCTC AAGGTAGGTT TTTTGATAGT AGAAAAGCGA 1080

TAGA 1084

( 2 ) INFORMATION FOR SEQ ID NO : 30 :

144

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1124 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE . TYPE : cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:

ATCGAATTCA TTGACTGCCT GAAAAGACTT CAACTCGTCT GCCTGATAAC CGAAAGACTT 60

GGTTACTTTG ATACCTGATA CGGACTCCTG TACCTTGTTA TTGAGTTCAG AAAAAGCAGC 120

TTGGGATTCG CCAAAGGCCT TATGAGTCTT TCTCCCTAGG CGACTAGTCG TATAGGCCAT 180

GAAAGGTAGG GGGAGAATGG CAACAAGAGT CATCTGCCAT GAGATGCTAA AGAGCATGGT 240

CAACAAAGTC ACCAGAGCCG TGATAGAGGC ATCCACCGCA GACATGACAC CGCCACCTGC 300

TAAACGAGTC AAGGAATTGA TATCATTGGT TGCGTGTGCC ATCAGATCAC CCGTCCGATA 360

GGTTTGATAA AAGGCTGACG ACATTTTTGT GAAATGCTTA AACAAGCGAG ACCGCATGAT 420

CTGTCCCAAG CAATAAGAGG TCCCAAGGAT ATACATACGC CACACATAGC GCAAATAGTA 480

CATACCAAAG GCTGCAAGTA GCAAGTAAAA TAGGCTAAGA AGGAGGTCCT GCTGGGTTAA 540

TTGCCCCGAT GTGATGGCAT CAATAACCCG CCCCATAACC ATAGGAGGAA TGAGATTGAG 600

GACGGAAACC AAGACCAGGG CCACAATCCC GACTAGATAA CGGCGTTTTT CTAACTTGAA 660

AAACCACCAA AATTTTTGAA TAATGGACAT AAAATCCCTT TCTGGATTGC AAATAGAAAC 720

CTGAGGCCAA TACTCAATGG AAAATCAAAG AGCAAACTAG GAAACTAGCC GCAGGCTGCT 780

CAAAGCACTG CTTTGAGGTT GTAGATAGAA CTGACGAAGT CAGTAACCTA CATACGGCAA 840

GGCGACGTTG ACGCCGTTTG AAGAAATTTC CGAAGAATAC AAGACCCCAG GTTTTTCTTA 900

TTTATAAGTT ACCACTGTAA CAGCACCCTT GTCATATTCA GCAATAAAGA TATTGGCTAC 960

ATTGTCATGC CCTTGTTTAC TGAGGTTATC AAGCAACCAC TCCTCGCTAC GAACAATCGA 1020

TCCCAAGACA TCTACTTGAA TCACACCGTC AGTCACAACT GGATACTTAG GATTTTCATC 1080

TCCCATTTGC ACAACGATGA GTTGCCCATT TTGCTCTTGC ACAG 1124

(2) INFORMATION FOR SEQ ID NO: 31:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1242 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:

TTACCTTCAT TGCAGCCATT ATTGGTTCTT GTGTCAGCCA GATTTTAAGT ATTCTTTATA 60

145

SUBSmUTE SHEET (RULE 26) AGACACCTGC TGTGGTCTTT ATCTTGGCCA TTTTGGCACC GCTGGTTCCA GGTTATCTCT 120

CCTACCGAAC AACTGCCTTT TTTGTGACAG GGGACTATAA TAAAGCACTG GCAAGTGCGA 180

CCTTGGTTGT CATGTTGGCT TTGGTAATCT CTATTGGAAT GGCTAGCGGA ACAGTGATTC 240

TCAGACTGTA TCATTATATA AAAACACATC GAGTATCGTA GACTTTACAG AAATAAAAGA 300

ATTTTCTGAA AAATGAGATA AATAAATTAA CAACGCTTTC TATATGTGCG AGAATACCGC 360

ACTTATGAAG AAATTGCGGC TGATTTTGGT ATCCACGAAA GCAACTTAAT CCGTCGGAGC 420

CAATGGGTTG AAGTAACTCT TGTTCAAAGT GGTGTTACGA TTTCAAAAAC TCATCTTAGT 480

GCTGAGAATA CGGTGATTGT GGATGCAACA GAGGTAAAAA TCAATCGCCC TAAAAAACAA 540

TTAGCGAATG ATTCTGGTAA AAAGAAATTT CACGCTATGA AGGCTCAGGC GATTGTCACA 600

AGTCAAGGGA GAATTGTTTC TTTGGATATC GCTGTGAACT ATTGTCATGA TATGAAGTTG 660

TTCAAAATGA GTCGCAGAAA TATCGGACAA GCTGGAAAAA TCTTGGCTGA TAGTGGTTAT 720

CAAGGGCCCA TGAAGATATA TCCTCAAGCA CAAACTCCAC GTAAATCCAG CAAACTCAAG 780

CCGCTAATAG CTGAAGATAA AGCTTATAAC CATGCGCTAT CCAAGGAGAG AAGCAAGGTT 840

GAGAACATCT TTGCCAAAGT AAAAACGTTT AAAATGTTTT CAACAACCTA TCGAAATCAT 900

CGTAAACGCT TCGGATTACG AATGAATTTG ATTGCTGGCA TTATCAATTA TGAACTAGGA 960

TTCTAGTTTT GCAGGAAGTC TATTATTTTC CTTATTGTCT GTAAGTCTAC TGACCTTGTT 1020

GTTTATCCCA GTCATGGTTT CTAGTTCGGG CTCAGAGTTT CAAAGTGGAT GGCAAGAGCA 1080

TCAATTGATT GCTGAGAAGG TTAGTAAAAC ACTTGACAAG ACATTTGATA AGGATGTCAG 1140

AAAAATTCCG ACCAGTCAGT TTTATCAAAA ATTTGTAGAT GAGATGGGAA GGATTTACTC 1200

AGGAAATTTG ATCCTCCCAG GAGCTGATAA CTGTGAATGG AG 1242

(2) INFORMATION FOR SEQ ID NO: 32:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1575 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:

GTGATGGGGC CTCAGGGAAA TGGTTTTGAC TTGTCTGACC TTGATGAGCA GAATCAGGTT 60

CTCCTTGTTG GTGGTGGGAT TGGTGTTCCA CCCTTGCTTG AGGTGGCCAA GGAATTGCAT 120

GAACGTGGAG TGAAAGTAGT GACAGTCCTC GGTTTTGCTA ATAAGGATGC TGTTATTTTG 180

AAAACGGAAT TGGCTCAGTA TGGTCAGGTC TTTGTAACGA CAGATGATGG TTCTTATGGC 240

ATCAAGGGAA ATGTTCCGTT GTTATCAATG ATTTAGATAG TCAGTTTGAT GCTGTTTACT 300

CGTGTGGGGC TCCAGGAATG ATGAAGTATA TCAATCAAAC CTTTGATGAT CACCCAAGAG 360

CCTATTTATC TCTGGAATCT CGTATGGCTT GTGGGATGGG AGCTTGCTAT GCCTGTGTTC 420

TAAAAGTACC AGAAAGCGAG ACGGTCAGCC AACGCGTCTG TGAAGATGGT CCTGTTTTCC 480

GCACAGGAAC AGTTGTATTA TAAGGAGAAA ATTATGACTA CAAATCGATT ACAAGTGTCT 540

CTACCTGGTT TGGATTTGAA AAATCCGATT ATTCCAGCAT CAGGCTGTTT TGGCTTTGGA 600

CAAGAGTATG CCAAGTACTA TGATTTAGAC CTTTTAGGTT CTATTATGAT CAAGGCGACA 660

146

SUBSTTTUTE SHEET (RULE 26) ACCCTTGAAC CACGTTTTGG GAATCCAACT CCAAGAGTGG CAGAGACGCC TGCTGGTATG 720

CTCAATGCAA TTGGCTTGCA AAATCCTGGT TTAGAGGTTG TTTTGGCTGA AAAGCTACCT 780

TGGCTGGAAA GAGAATATCC AAATCTTCCT ATTATTGCCA ATGTAGCTGG TTTTTCAAAA 840

CAAGAGTATG CAGCTGTTTC TCATGGGATT TCCAAGGCAA CTAATATAAA AGCTATCGAG 900

CTCAATATTT CTTGTCCCAA TGTTGACCAC TGTAATCATG GACTTTTGAT TGGTCAAGAT 960

CCAGATTTGG CTTATGATGT GGTGAAAGCA GCTGTGGAAG CCTCAGAAGT GCCAGTTTAT 1020

GTCAAATTAA CCCCGAGTGT GACCGATATC GTTACTGTCG CAAAAGCTGC AGAAGATGCG 1080

GGAGCAAGTG GCTTGACTAT GATCATACTC TGGTGGGATG CGCTTTGACC TCAAAACCAG 1140

AAAACCAATC TTGGCCAATG GAACAGGTGG AATGTCAGGT CCAGCAGTTT TCCAGTAGCC 1200

CTCAAACTCA TCCGCCAAGT AGCCCAAACA ACAGACCTGC CTATCATTGG AATGGGGGGA 1260

GTGGATTCGG CTGAAGCTGC CCTAGAAATG TATCTGGCTG GGGCATCTGC TATCGGAGTT 1320

GGAACAGCTA ACTTTACCAA TCCTTATGCC TGCCCTGACA TCATCGAAAA TTTACCAAAA 1380

GTCATGGATA AATACGGTAT TAGCAGTCTG GAAGAACTCC GTCAGGAAGT AAAAGAGTCT 1440

CTGAGGTAAA CTGCAATCAA TCTGTTCTTG ATTTTTTATT AGTTTGTAAT ATGAATTTAG 1500

GAGAATTTTG GTACAATAAA A AAATAAGA ACAGAGGAAG AAGGTTAATG AAGAAAGTAA 1560

GATTTATTTT TTTAG 1575

(2) INFORMATION FOR SEQ ID NO: 33:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 776 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:

CTAAGATATC AGAATAACAA CGAAATCGAA GCATTAAAAA CAAATATTAC TTCTAAGAAT 60

AGCGAGATTG ATAGTCAACA AAGCAATATT AAGGATATGA CCGTACCTAT AATGATCCAA 120

CTTCTCAGGC TTATAATATT TATGCTCAAT TAATTAGTGA GTTAGGTACT GCTCGTTCAA 180

ACAACAATAA AAGTATTACA GAGCTTGAGG CTAATCTTGG AGTGGCAACA GGTCAAGATA 240

AAGCTCATAG TATATTAGCG TCAAATGAAG GTACTCTGCA TTATCTGGTA CCTTTGAAAC 300

AAGGAATGTC TATTCAGCAG GGGCAAACGA TAGCAGAAGT TTCAGGGAAA GAAAAAGGTT 360

ACTATGTAGA GGCTTTTGTA CTTGCGAGTG ATATTTCTCG TGTTTCAAAA GGAGCAAAAG 420

TTGATGTTGC TATTACTGGT GTGAATAGTC AAAAATATGG AACACTAAAG GGACAAGTCA 480

GACAGATTGA TTCAGGAACA ATTTCCCAAG AAACGAAAGA GGGGAATATT AGCCTCTATA 540

AAGTCATGAT AGAATTAGAA ACCTTAACTC TAAAACATGG AAGCGAGACG GTCATACTCC 600

AAAAGGATAT GCCAGTTGAA GTGCGGATTG TCTATGATAA AGAAACCTAT CTTGATTGGA 660

TTTTAGAAAT GTTAAGTTTC AAGCAATAAT TGGTTTTAAA CCTTAGGTAA CCTATAAAAA 720

CAAATAAGGT AGAGAAAGGA TATTTTATCT AAGTTAGCTC ACATTACTGC CATTCC 776

(2) INFORMATION FOR SEQ ID NO: 34:

147

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1487 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34:

CTGGCCTTTC TCCACCAAAA TTGTTCCTTG AGGGAAGGAA GTCAGAACAC TAGCCGTTGC 60

ATCTTCCTTT TGCTTTTCAA TCGTAATTCC AGATAATTTT TCCCATTCTT TTTGGTGACC 120

CCGGGAGGCA GGATTGAATG GCTTGAGGGA AATGACAAAC TTGTCCTAGC AAGAATGGTC 180

AAGGCACCTC CGTCTACAAT CAAAATCTGA TTTGGGCTTA AATTAACAAA GACCTGTTTT 240

ACTAGATTTT CTCCAGAAGC ATCGTCTCGT AAACCAGGCC CCAGCAAGAT AACTTCTGCC 300

TTCTCCAATT GCTCTTTTAA CAATTGCTGG TCTTGAAGAG AAAAGGCCAT AGGCTCAGGT 360

AAATGGCTGT GCAGAGCCGG GATATTTTCC CTGTCCGTTC CAACGGTCAC CAATCCTGCA 420

CCGCTTTTTA CAGCTGCTAA AGCAGCCATG ATGATGGCAC CTCCATAAGG ATAAGTACCA 480

CCAAGCAGCA GCAGACGACC ATAATCTCCT TTATGACTTG AACGAGAACG TTCAATAATA 540

ACTTTTTCTA GTAAGGTTTG ATTAATCACT TTCATCCTTT TTCCCTCTCA CTTTTATTAT 600

ACAACAAAAA GGAGACGCAG ACCTCCTTTT GTAATCTTAT ATCTAAAATT TAATATTCAT 660

TTCTGCCATT TTAGATA AG CTATAGAAAA TACACTCTAT TAATCGAATG TTTCTCTTAT 720

TTTCTATCCA ATGTCCGAAG TGCTGCTTGA TAAGTTTGCT CCATCAGCAT GGTAATGGTC 780

ATAGGACCGA CACCTCCAGG GACTGGCGTG ATATGGCTAG CAAGTGGTGC AACTGCCTCA 840

TAATCAACAT CTCCACAGAG CTTCCCATTT TCATCTCGGT TCATCCCAAC GTCAATGACA 900

ACCGCACCTG GTTTGACAAA GTCAGCAGTC ACAAACTTGG CGCGGCCGAT TGCGACTACA 960

AGAATATCTG CTTTAGCAGC CACCTTGGCA AGATTATGAG TTCGTGAGTG GGCCAAGGTT 1020

ACTGTCGCAT TTTTAGCCAA AAGAAGCTGA GCCATAGGTT TTCCAACGAT ATTTGAACGA 1080

CCGATTACGA CCGCATTTTT ACCTTCCAAG TCAATCCCAT ATTCATGAAA CATTTCCATA 1140

ATTCCTGCAG GTGTCGAGGG AATCATGACT GGATGTCCAG ACCAAAGACG TCCCATGTTT 1200

AGGGGATGGA AACCATCCAC ATCCTTTTCT GGGTCAATGG CTAATAAAAC CGCCTCTTCA 1260

TCGATATGTT TTGGTAATGG CAACTGGACC AAAATCCCAT GCCAAGCTGG ATCCTGATTA 1320

TATTTAGCAA TCAGGTCTAA CAATTCCTCT TGAGTAATGG TCTCTGGAAC TCGCACTACT 1380

TCGGTACGGG AACCAGCCGC AAGAGCTGAC CTCTCCTTGT TGCGAACGTT AAACTTGGCT 1440

GGCTGGATTA TCCCCAACCA AAATCACTAC CAAACCAGGC ACTAGAG 1487

(2) INFORMATION FOR SEQ ID NO: 35:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1634 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

148

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35:

CGTGCCTTGG CCAATGATCC AAAAATCTTG ATTTCAGACG AGTCGCTTCA AATTTCGGCC 60

CCTGGACCCT TAAGACCAAC CCAAGCAGAT TTTGGCCCTT GGTTGCAAGA TTTGAACCAA 120

AAATTAGGCT TGACTGTTGT CCTGATTACG CATGAAATGC AGATTGTCAA AGACATTGCC 180

AACCGTGTTG CAGTTATGCA GGATGGGCAT TTGATTGAAG AGAGTAGTGT GCTTGAAATC 240

TTCTCAGACC CTAAACAACC TTTGACTCAA GACTTTATCT CAACAGCTAC AGGTATTGAC 300

GAAGCCATGG TCAAAATCGA GAAGCAAGAA ATCGTGGAAC ACTTGTCTGA AAACAGTCTC 360

TTGGTGCAAC TCAAGTACGC TGGATCTTCA ACAGACGAGC CACTTTTGAA TGAATTGTAC 420

AAGCATTATC AAGTAATGGC TAATATTCTC TATGGGAATA TCGAAATCCT CGATGGTACT 480

CCTGTTGGAG AATTGGTGGT GGTCTTGTCA GGTGAAAAAG CAGCGCTGGC AGGTGCTCAA 540

GAAGCCATTC GTCAAGCAGG CGTACAGTTA AAAGTATTGA AGGGAGGACA GTAAGATGGA 600

ATCATTGATT CAAACCTATT TACCAAATGT CTATAAGATG GGTTGGTCTG GTCAGGCAGG 660

CTGGGGAACA GCTATCTACC TAACCCTCTA TATGACAGTT CTTTCCTTCA TTATCGGAGG 720

CTTCTTGGGG CTAGTGGCAG GTCTCTTTCT CGTCTTGACA GCGCCAGGTG GTGTCTTGGA 780

GAATAAAGTC GTATTCTGGA TTTTAGACAA AATTACCTCA ATTTTTCGTG CGGTTCCCTT 840

TATCATCCTC TTGGCAATCT TGTCACCACT TTCTCACTTG ATTGAAAAAA CAAGTATCGG 900

GCCAAATGCA AGCCCTTGTC CCACTTTCTT TTGCAGTCTT TGCCTTCTTT GCCCGTCAGG 960

TGCAGGTTGT CTTGGCTGAA ATGGATGGCG GTGTCATTGA GGCGGGCTCA AAGCGAGCGG 1020

AGCGACTTTC TGGGACATCG TGGGTGTTTA CCTATCAGAA GGTCTTCCAG ATTTGATCCG 1080

TGTGACGACT GTGACCTTGA TTTCCCTTGT TGGGGAAACA GCTATGGCCG GTGCGGTTGG 1140

AGCTGGTGGT ATCGGTAACG TAGCCATCGC TTATGGATTT AACCGCTACA ATCACGATGT 1200

GACCATCTTG GCAACCATCG TTATCATTTT GATTATCTTT GCAATCCAAT TCTTAGGAGA 1260

TTTCTTGACT AAGAAATTGA GCCATAAATA AAAAAGAGCC GTGTGGCTCT TTTTAACTGA 1320

TCAGATTTTC TGGGCAAATT TTTTACTCAA GGCTTGTCCA ATCAAGGCAC CCACTAGGGC 1380

TCCGATGACA ATACTTGCGA TAAATAGAAG GACAGTTCCA GGGTTTGGAG CGACCATGAT 1440

GCGGTCGATA TATTCTTGGG ATTTTCCTCT TGCCAGAAGA GTAGCCATAT AGGCTTTGGG 1500

CGCAATCCAC ATAAGCAAGA TTGGTCCTGT TGTACTAAAG GCGAAAATAA TGAAAGAAAG 1560

GAAGTTCTTT GTTTTGTCCT TGTATTTTCC TAAATGAGCT ACTCCATCTG CTAGGAGGCC 1620

ACAGATAATT CGAT 1634

(2) INFORMATION FOR SEQ ID NO: 36:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1087 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36:

149

SUBSTTIUTE SHEET (RULE 26) GGAATCATGA TGATGTCACT GCTAAATGGT TTCTTAGAAA AAATATTTCC TGAGCGCTTA 60

CAGATTAGTT TGGGCTTGCT GATTTTATCA TTGAGCGGTA CAGCTCCCTT CTGGTACCAA 120

GCCTATCCCT TTGTCTTTGG AACACGGCTT CTCTTTGGTT TGGGTCTTGG GATGATCAAT 180

GCCAAGGCCA TTTCTATTAT CAGTGAACGC TACCAAGGAA AAAGGCGAAT TCAGATGTTA 240

GGGCTACGCG CTTCTGCAGA GGTCGTTGGA GCTTCTCTCA TTACCTTGGC CGTCGGTCAA 300

GTTGTTGGCC TTTGGTTGGA CAGCTATCTT TCTAGCCTAT AGTGCTGGAT TTTTGGTGCT 360

GCCCCTT AT CTGCTCTTTG TCCCTTATGG AAAATCAAAG AAAGAAGTCA AGAAAAGAGC 420

GAAGGAAGCA AGTCGTTTAA CTCGAGAAAT GAAAGGCTTG ATTTTTACCT TAGCTATCGA 480

AGCGGCAGTT GTAGTTTGTA CCAATACAGC TATTACCATC CGTATTCCAA GTTTGATGGT 540

GGAAAGAGGA TTGGGGGATG CCCAGTTATC TAGTTTTGTT CTTAGTATCA TGCAGTTGAT 600

CGGGATTGTG GCTGGGGTGA GTTTTTCTTT CTTGATTTCT ATCTTTAAAG AGAAACTGCT 660

CCTCTGGTCT GGTATTACCT TTGGCTTGGG GCAAATCGTG ATTGCCTTGT CTTCATCCTT 720

GTGGGTGGTA GTAGCAGGAA GTGTTCTGGC TGGATTTGCC TATAGTGTAG TCTTGACGAC 780

GGTCTTTCAA CTTGTCTCTG AACGAATTCC AGCTAAACTC CTCAATCAAG CAACTTCATT 840

TGCTGTATTA GGCTGTAGTT TCGGAGCCTT TACGACCCCA TTCGTTCTAG GTGCAATTGG 900

CTTACTAACT CACAATGGGA TGTTGGTCTT TAGTATCTTA GGAGGTTGGT TGATTGTAAT 960

CTCTATCTTT GTCATGTACC TACTTCAGAA GAGAGCTCTA GGATTGATTC CTAAGTTTTT 1020

CTTTTGATAC TCAATGAAAA TCAAAGAGCA AACTATAGTT GATTGAGTTT GGAATAGTAT 1080

GCTGTAG 1087

(2) INFORMATION FOR SEQ ID NO: 37:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1191 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37:

GGATTCCAAC GATTATGAAC TTGACTGGTC CACTGATTCA TCCAATGGCT TTAGAAACAC 60

AGCTTTCTTG GAATTAGTCG TCCAGACTCC TAGAAAGTAC AGCTCAGGTT TTGAAAATAT 120

GGTCGCAAAC GTGCCATCGT GGTTGCTGGA CCAGAAGGGT TGGATGAAGC TGGCTTGAAC 180

GGAACAACCN AGATTGCACT TNTTGAAAAT GGCGAAATCA GCTTGTCAAG CTTTACTCCA 240

GAGGATTTGG GAATGGAAGG CTATGCTATG GAAGATATTC GTGGTGGGAA TGCTCAGGAA 300

AATGCAGAAA TTTTGCTTAG CGTTCTGAAA AACGAAGCAA GTCCATTCTT GGAAACGACA 360

GTCTTGAATG CTGGTCTTGG TTTCTATGCT AATGGTAAGA TTGATAGCAT CAAGGAAGGA 420

GTTGCCTTGG CCCGTCAAGT GATTGCTAGA GGCAAGGCCC TTGAAAAACT CAGACTGTTA 480

CAGGAGTACC AAAAATGAGT CAGGAATTTT TAGCACGAAT CTTAGAGCAG AAGGCGCGTG 540

AGGTGGAGCA GATGAAGCTG GAGCAAATCC AGCCTCTGCG CCAGACCTAT CGCTTGGCAG 600

AATTTTTGAA GAATCATCAG GACCGCTTGC AGGTAATCGC TGAGTCAAGA AAGCTAGCCC 660

150

SUBSTTTUTE SHEET (RULE 26) TAGTTTGGGA GATATCAATC TCGATGTGGA TATTGTGCAA CAGGCCCAGA CTTATGAAGA 720

AAACGGAGCA GTGATGATTT CGGTGTTGAC AGATGAGGTT TTCTTTAAAG GGCATTTGGA 780

TTATCTACGG GAAATTTCCA GTCAGGTAGA GATTCCGACG CTCAACAAAG ACTTTATCAT 840

AGATGAAAAG CAAATCATCC GCGCTCGCAA TGCAGGTGCG ACAGTTATCT TGCTTATTGT 900

GGCAGCCTTG TCCGAAGAAC GCCTCAAGGA ACTGTATGAC TACGCGACAG AGCTTGGTCT 960

GGAAGTCTTA GTGGAGACTC ACAATCTAGC TGAACTAGAG GTAGCCCACA GACTTGGTGG 1020

CTGAGATTAT CGGGGTCAAC AACCGCAACT TGACTACCTT TGAAGTCGAC TTGCAGACCA 1080

GTGTAGATTT AGCCCCTTAC TTTGAGGAAG GTCGCTATTA CATTTCTGAA TCTGCCATTT 1140

TCACAGGGCA GGATGCGGAA CGACTAGCCC CATACTTTAA CGGAATTCGA T 1191

(2) INFORMATION FOR SEQ ID NO: 38:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 858 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38:

ATCGAATTTG CCAACCAAGA AAAATATCCC TTGGATGGTT CTTGGCAATG CAAGCAATAT 60

CATCGTTCGT GATGGTGGGA TTCGTGGATT TGTCATCTTG TGTGACAAGC TCAATAACGT 120

TTCTGTTGAT GGCTATACCA TTGAAGCAGA AGCTGGGGCT AACTTGATTG AAACAACTCG 180

CATTGCCCTC CGTCATAGTT TAACTGGCTT TGAGTTTGCT TGTGGTATTC CAGGAAGCGT 240

TGGCGGTGCT GTCTTTATGA ATGCGGGTGC CTATGGTGGC GAGATTGCTC ACATCTTGCA 300

GTCTTGTAAG GTCTTGACCA AGGATGGAGA AATCGAAACC CTGTCTGCTA AAGACTTGGC 360

TTTTGGTTAC CGCCATTCAG CTATTCAGGA GTCTGGTGCA GTTGTCTTGT CAGTTAAATT 420

TGCCCTAGCT CCAGGAACCC ATCAGGTTAT CAAGCAGGAA ATGGACCGCT TGACGCACCT 480

ACGTGAACTC AAGCAACCTT TGGAATACCC ATCTTGTGGC TCGGTCTTTA AGCGTCCAGT 540

CGGGCATTTT GCAGGTCAGT TCGAATTTCA GAAGCTGGCT TGAAAGGCTA TCGTATCGGT 600

GGCGTAGAAG TGTCAGAAAA GCATGCAGGA TTTATGATCA ATGTCGCAGA TGGAACGGCC 660

AAAGACTACG AGGACTTGAT CCAATCGGTT ATCGAAAAAG TCAAGGAACA CTCAGGTATT 720

ACGCTTGAAA GAGAAGTCCG GATCTTGGGT GAAAGCCTAT CGGTAGCGAA GATGTATGCA 780

GGTGGTTTTA CTCCCTGCAA GAGGTAGTGG GGACCTGACA GAGCCCCGAT CGGTTAATCT 840

ATGAAAAAGA AGGAATTT 858

(2) INFORMATION FOR SEQ ID NO: 39:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 980 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

151

SUBSTTTUTE SHEET (RULE 26) (D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39:

CTGAAAAAAC AGGTTTTGAC TATGNAGATT GACAGACGAC CGTTCGGAGG TGCAGATATT 60

GATGCAGCAG GACCTCCCTT ACCTGATGAA ACCCTTAAGG CAAGTAGGGA AGCAGATGCT 120

ATCCTACTAG TAGCTATCGG TAGTCCTCAG TATGATGGAG TAGCGGTTCG CCCTGAACAA 180

GGCCTGATGG CTCTCCGTAA GAACTCAATC TTTACGCTAA TATTCGTCCT GTAAAAATCT 240

TTGACAGTCT CAAGTATTTG TCACCACTCA AACCGGAACG AATTTCTGGT GTAGACTTCG 300

TCGTGGTGCG TGAATTGACT AGGCGAGATT TACTTTGGAG ATCATATCCT TGAAGAGCGC 360

AAAGCGCGTG ATATCAACGA CTATAGCTAT GAGGAAGTGG AGCGGATTAT TCGCAAAGCC 420

TTTGCCATCG AATTGCAAGA AATCGCAGAA AAATCGTTAC TAGTATCGAT AAGCAAAATG 480

TTCTAGCGAC CTCAAAACTC TGGCGGAAAG TAGCTGAGGA AGTCGCACAG GATTTCTCAG 540

ATGTAACCTT GGAACACCAG CTGGTAGACT CAGCTGCTAT GCTTATGATT ACCAATCCTG 600

CTAAGTTTGA TGTTATTGTA ACGGAGAATC TTTTTGGAGA TATTTTATCT GATGAATCAA 660

GCGTCTTATC TGGTACACTT GGGGTTATGC CATCAGCCAG TCATTCTGAA AATGGACCAA 720

GTCTCTATGA ACCTATTCAC GGTTCAGCAC CTGATATTGC AGGTCAAGGA ATTGCCAATC 780

CTATTTCCAT GATTTTATCA GTTGTCATGA TGTTGAGAGA TAGTTTCGGA CGTTATGAGG 840

ATACAGAGCG TATCAAACGT GCTGTTGAGA CAAGTCTGGC GGCAGGAATT TTAACGAGAG 900

ATATAGGAGG TCAGGCTTCA ACAAAGGAAA TGATGGAAGC TATTATTGCA AGGTTATGAA 960

GTTAGACGAA AAAATTCGAT 980

(2) INFORMATION FOR SEQ ID NO: 40:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 874 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 40:

TCGATCTAGA GAATTGCTCC AGAGCTTCCT GACCGTCCGC TGCCTCAATA GTTTCATAGC 60

CACAATCCGT CAAATAATCA CTGACCCCCT CACGGATCAT CTCTTCATCT TCTACAATTA 120

AAATTTTCAT ACTTTAACTG CTCTCTATTT TTTATTTTTC TTAGAATAAA TACCTACTCT 180

ATTTTCTATT ATAGTCTCTT GCTGGCCTTT TGTATGTAAG CAACTGACCA CTAGATAAAA 240

CGTTGTGAAA TTCCTTTCTC ATAAATTCCA TAACTTTAGT ATATTATATT TAAGCACTAA 300

AGTACAAAGA AAGCAACTGA AAGCAATGAT TTTCACCACT GCTTTCAGAT TTATTTTGAA 360

TTGTTAAATA GCTATTCCTA TCCACTATTC TTGAATAGAA ACACAAGATG CAATCTTTAT 420

TCCAGACTCA TTTTTTAAAA AATCAAATTT ATTCACCATC CAGCAAGAGC TCTTTTGGTT 480

152

SUBSmUTE SHEET (RULE 26) GTTTTCTAAG GAGATTGCTT GAAGCAAGCG CCATAACGAG AACCACTAGA ACCAAGGCAA 540

GGACAAAAAT GATGATAAAG TCTGATGTCT GAATGGAAAT GTCTAGGCTC GACAAGGTCT 600

TGCTAAAGCC ATCTACTTCT GCACCGCCAC CAAGGTTAGA GGCTTGAGCC GCCTTACTAG 660

CCTGTTTGGC AACACCTGAA GTCACATTGG CAAGGACAGT GTTTCCAATT CGCACGGGCA 720

GTGTAATTAG CTAGGAAGTA AGCANAAACT AGAGCAGGGA TAGCAATCAA GATAGATTCG 780

GTGATGAATT GACCCAAGAT ACTTGCCTGC TTGAGACCAA TAGAGAGGAG GATTCCCACT 840

TCCTTGCCGA CGGGCATTGA TCCAAAGACT GAGC 874

(2) INFORMATION FOR SEQ ID NO: 41:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 762 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41:

CTTGTAACGG TCATAAAGTT TCTGCAAACT ACCATCCTTG CTCCATTTAG TAACCAAGTT 60

ATCAAGATAG TCGTTGAGCT CTGTATTTGA TTTCTTGGTA ACAATACCGT AGTCAGATGG 120

CTTGAAACTA TCATCTAGTA GTTCTGTGCG TTTAACTAGT GTAGCCAGAT AGAATAGAGC 180

GGTCAACGGA AAAGGCATCG ATACGATGAG CGTGAAGGGA AGTAATCAAT TCTGGGTAGG 240

AACCAAGTTC GACGAATTTA AACTTCAGAC CTTTCTTTTT ACCCAGTTCA GTAATCAGGC 300

GTTGGGTGAT AGAACCTTGG GCGACTCCGA TGGTTTTGCC GTTTAGGTCC TCAATCTTTT 360

TGATTTTGGC AGATTTATTG ACCAAAAATC CAGAAGCGTC TGTGTAGTAG GGACTGGTAA 420

AGTTGTAGAG TTTTTTGCGT TCGTCCGTGA TGGTAAAGGT CGCGATATCC ATATCGACCT 480

GTTCATTGTC TAGAAGGGGG CCGCGGGTTT GTGCTGTAAC CGGCACATAG TGAATCTTGA 540

CCTTGAGTTC ATCAGCTACC ATTTTGGCCA AGTCGGTTTC GATACCAGAA TAAGTACCGG 600

TCTTGGGATC TTTGTTAACC AAAATTGGGA ACGTCTTGTT TGACACCCGA CAACCAGTTC 660

GCCTCTTTTT TGAATGTCTG CGATACTAGT ATTAGCCTGG ACTGGTTTGG CAGCAACAAG 720

GCCGAAAAGG CTAATCAATA ATGCTGATAA AAAGAATTCG AT 762

(2) INFORMATION FOR SEQ ID NO: 42:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1942 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:42:

153

SUBSTTTUTE SHEET (RULE 26) CTCGAATTTT TGGTGCTCCA GAAACGGTTC CAGCAGGAAG CGTTGCTTTC AAGGCATCCA 60

TGGCAGTGAG TTCTGCAAGC AAACGTCCCT TGACCACACT GGTCAAATGC ATGACGTAGC 120

GGAAGAGCTC CACCTCCATA TACTTAGTAA CTTGGACACT GGCCGTTTCA GAGATGCGGC 180

CAATATCGTT ACGCCCCAAG TCTACCAACA TTCGATGTTC TGCTGTTTCC TTCTCATCAG 240

AGAGGAGGTC AGTCGCCAAG GCCTTGTCTT CTCCATCCGT AGCCCCTCTT GGTCGCGTCC 300

CTGCAATCGG ATTGGTTGTC ACGATGCCAT TTTTGACAGA AACCAAACTT TCTGGACTAG 360

CTCCGATGAT TTGATAATCC CCAAAATCAT ACAAATAAAG GTAATTAGAT GGATTAGTCA 420

CGCGGAGATT TCTGTAGAAG TCAAATGGAT TTCCAGTTAA CTTCTGCGTG AAGAAAACGC 480

TGGCTGAGTT ACACATCGGA ACATATCTCC GTTACGAATC AAGTCACGAG CTGTTTCTAC 540

CATTCCCTCA AACTTATGTG GAGCGATATG CGGTTTGAAG TCAAGTGGTG ATAAATCCAA 600

GTCTTCAAAT TCATTTGGAG CAGGAATGCG TAATTCCTCA AGCACTTGGT TCAAGGATTT 660

TTCCAAGGCC TCTTGACTGC GCTCACTATA AAGTGCATCC TCTATGACAT GTTATCTTCT 720

CCTTCTTGTT GGTCAAAGAC CATATAGCTC TCATAGACAA AGAAATGCAT GTCGGGCGTC 780

CCAATTGTAT CCTCAGGGAT TTGACCAATT TCTTCATAAA GCGAAATCAT ATCGTAACCA 840

ACAAAACCAA TGGCTCCCCC ACCAAAAGGG AGGTCTGAAT GGTGCTGGCT CTTATGAATC 900

ACTTCATAAA GGAAATCCAA GGGATCCCGA TCAATCGCTT GACCATTTTG ATAGAGAACT 960

CCATTTTCAA ACTTAATCTC AAAAACTGGA TTATAGGCTA GGATAGAAAA ACGAGCTGTT 1020

TCCTTGTCTC TCGGAATACT CTCTAAAATA ACCTTATGTT GCCCCTTTAA GCGCATATAA 1080

GCCAAGATTG GTGATAAGAC ATCTCCATGA ATGATTCGTT CCATTGTCAT TTCCCTTTCA 1140

GTTCTAATTC GAGTTCGTGG CGACTGTATG AAAAATCCCC ACGCAAAATA ACTTGCGTGA 1200

GGACGAAATT CGCGGTGCCA CCTCAATTAT AGGATTTCTC CTATCTCTCA TTCCTGTCTC 1260

AGATATCTCC TGTAACAGGC TGTGCGATAA AGGGCACTCC CTTGAGAATG ATGTTTTCTT 1320

CTCTCGTTTC AGATGAACCC AACTTTACAG CTTTCTCTGC TTGTTTTCAG CAACCACAAG 1380

CTCTCTGTGA GAGAAAAGAC TGTAATTTTT CCATCTATTA TTTTTTAGCT TCTAGTAATC 1440

TGCAATCGCA GCTAGGTCCT TGCCTCCACG ACCAGAGACA TTGATGAAGA GATGTTCATC 1500

TCGGTACACC TTTATACTCT TCGAAAATCT CTTCAAACCG CGTCAACGTC GCCTTGCCGT 1560

AGGTATGGTT ACTGACTTCG TCAGTTCTAT CTGCAACCTC AAAACAGTGT TTTGAGCTGA 1620

CTTCGTCAGT CTTATCGACA ACCTCAAAAC AGTGTTTTGA GCAGCCTGCA GCTAGTTTCC 1680

TAGTTTGCTC TTTGATTTTC ATTGAGTATT ATTTCATTTT CTCCTGCAAT TGAATTCTTG 1740

CTCAGCTTTT TGTCTTCTAT TTCTTTAAAA TCAAAGTAGC TCTTTTGTTA ATAACTCGAT 1800

CAACAAACAT CGTGGTACAA GTATCTACTT TGAAATTTAT CAACCACTTA ACAACTGATA 1860

CTGTATTTCT AGGAAAACGA TGACATTCTT CCTAATAAAA CTTCTCATAT ATAGCATAAA 1920

TTTCTACTCT TTTTAATTCG AT 1942

(2) INFORMATION FOR SEQ ID NO: 43:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1048 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

154

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:43:

CTGTTAAGAT TGTTTCCGTG CATCCACATA GGATTTACCT TGTCTGTATG GGCCAATTCA 60

CCCATCAAAA CGCCATAGGT CTCATCTGTC AAGATACTAG ACATACCGAT ATTGTACCAA 120

AGACTGGTAT GACGGAAATA AGTCGATGCG TGTAAACTCA ACAAAAAGAG ACGCAAGTTG 180

ATTAGAAAAA CCGTCATAGC AATAGCTGCC ACAGGAGCTT GAACCACAAT CAGTGCCAAC 240

ATGGCAAACT GGGCACTCCC AGCATAAACA AAGAGACTCA TCAAGCCCAT CTCAACAGGT 300

GTCACATAGG GCGCACCGAT AGTCCCACAG GCCAGGCCGA TACTGACATA GCCAAGAGCC 360

GTTGGCATGG CTGCCTGCGC CCCCTCCTAA AATCCTTTTT CTTTCATCTT TCTCCTCATA 420

TTGTCTTAAT AATACTCAAT GAAAATCAAA GAGCAAACTA GGAAATTAGC CGCAGGNTGC 480

TCAAAACACC GTTTTGAGGT TGCAGATAGA AACTGACGAA GTCAGCTCAA AACACCGTTT 540

TGAGGTTGCA GATAGAACTG ACGAAGTCAG TAACATATAT ACGGCAAGGC GACGTTGACG 600

TGGTTTGAAG AGATTTTCGA AGAGTATTAG AAAATGCCGA TAAGGGTCTG CATACCAAGG 660

CTGGTGAGGA TGATGGCAAT CCAGCAGACG GCTCCGAGAA CAATGGATTT TCCACTGGAT 720

TTGACCATAG CGACCAGATT AGTTTTGAGA CCGATGGCAC TCATGGCCAT GATAATGAGG 780

AATTTAGAGA GTTGTTTGAG AGGGGTAAAG AAACTACTAG ACACACCGAG AGAGGTCAGA 840

AGGGTGGTTA GGAGCGATGC AAGGATGAAG TAAAGGATAA AAAGTGGGAA GACTTTTTTC 900

AGTTGTAAGC CTTGCTTATT TTTTTGCTCG CGACTTTGCC AGTAGGAGAG AAAGAGAGTG 960

ATGGGGATGA TAGCTAGGGT GCGCGTGAGT TTGACAATGG TTGCGGATTC GAGGGTATTG 1020

GTCTGGTAGA GACTGTCCCA AGCGCTAG 1048

(2) INFORMATION FOR SEQ ID NO: 44:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1571 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 44 :

AGAGCTGGTA ATATTCCCAA AGAAACGGCT CAAATCGAAT TAGAAAGCCT TCTGCAAAAA 60

GGAATCCCAG TCGCTCTGGT ATCACGATGC TTTAACGGTA TTGCCGAGCC TGTTTATGCC 120

TACCAGGGTG GGGGCGTACA GTTGCAAAAA GCAGGCGTTT TCTTTGTTAA AGAACTCAAC 180

GCCCAAAAAG CCCGCTTGAA ACTCCTCATC GCCCTCAATG CCGGACTAAC AGGACAGGCT 240

TTGAAAGACT ATATGGAAGG CTAATACTCT TCGAAAATCT CTGCAAACCA CGTCAGCGTC 300

GCCTTACCGT ATGTAGAGCA CAAAATCAGG AAATCTTCTC GATTCCCTGA TTTTTTCTAT 360

TTACGTTTTC GTGTTGAGCT ACGTTCTGTC AAACCATGAG GTAAGAGAAC TTCACGTTCT 420

TCCAACTCTT CCTTATGCAT AATCTTGGTC AACATACGCA TACTAATGGC ACCAAGGTCA 480

TAAAGAGGTT GGGCAATCGT TGTCAAGTTT GGACGGGTAA AGCGTGAGAT TTGTGAATCA 540

TCACTAGTAA TAATTCGATA ATCTTCTGGC ACAGAAACAC CTTATCAGCC AAACCGTTCA 600

155

SUBSTTTUTE SHEET (RULE 26) AGACTCCTGC TGCCAACTCA TCACCTGTCA CAACTGCTGC AGTTGCATTT GATGAAATCA 660

AACGCTCTGC TAAGGCGTAA CCATCATCAT AGCTATATTT AGATTCAAAT ACCAAACCCT 720

CACTATAAGC GATTCCTGCT TTTTTCAAGG TTTCCTTGTA GCCAACTAAA CGAACCTTAC 780

CATTGATGTC ATCCACTAGC GGACCGCTAA CGAAAGCAAT ACGCTCATTT TCTTTAGCAA 840

GGTAACTCAC TGCATCAATT GTTGCTTGCT TATAGTCAAT ATTGACACTT GGCAACTGGT 900

GCTCAACATC GACAGTTCCT GCGAGAACAA TCGGAGTACG TGAACGCGAA AATTCTGAGC 960

GAATTTTATC TGTCAAGTGA TAACCCATAT AGATAATGCC ATCTACCTGC TTTGAAAAGA 1020

GGGTATTGAC AACAGAAACT TCTTTCTCGT TATCTTCATC GCTATTAGCT AGGACAATAT 1080

TGTACTTGTA CATTTCTGCA ATATCATCAA TCCCCTTAGC CAAACTCGAA AAATAACCAT 1140

TGGTAATATT TGGAATCACG ACACCGACAG TGGTTGTCTT TTTACTTGCA AGACCACGCG 1200

CAACTGCATT TGGACGATAA TCCAAACGAT CAATTACCTC TAGCACTTTT TTACGGGTAT 1260

TCTCTTTTAC ATTTTTATTG CCATTGACCA CACGGCTGAC CGTCGCCATG GGAAACACCT 1320

GCTTCACGAG CGACATCATA AATGGTTACT GTATCATCTG CATTCATTCC TTTTCCTGTC 1380

CTTTCTATCT CCACACATTC TTTTACAAGT AGAAGTGCTG AATTGAAAGC TCTATATCTT 1440

ACTTACAAAA ATGAAGATGT GAAAATTTCG TTTTCA ATT TCTACTTATT CCATTCTATC 1500

ACTAATTGTA AACACTTTCA AGTGTTTTTT GAAGATTGAT TGAAAAAATT TCATAGAAAA 1560

CCTAGGTTTA G 1571

(2) INFORMATION FOR SEQ ID NO: 45:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1682 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45:

CTGACGTAAA AAAGATTTTC GGAAAAGTAT CATCATCTAT TTTAGACCAT TTTCTTATAA 60

TAACCATTTT ATTTTTATTT GTCAAGGTCT TTGAATTCTT TCTTAAACAA GCCTTGTAAT 120

CTCTACTTTT GAAGAATTTA TTTTTCCTTA CTGACAAGAT TTGAGACGGT AGGAATCATT 180

GAAAATAACC TAGCCAACAT CAATCACAAT CATTTCTCCT TTCTCAATTA CACTAAATTA 240

TAGTGTATTG AATCTATAAC AGTGCACCTT GGCTGCTAAA ATATTTCTAT AAATTAATTT 300

GACTTTCCTG ATAGAGTTGT TCACATCTTA TTTCAATTCA CTATACTTTC CCTTATACTC 360

AATGAAAATC AAAGCGCAAA CTAGGAAGCT AGCCACAGGC TGCTCAAAGC ACTGCTTTGA 420

GGTTGTAGAT AAGACTGACG AAGTCAGTTA CATATATCTA CGGCAAGGCG AAGCTGACGC 480

GGTTTGAAGA GATTTTCGAA GAGTATAAAG TTTGTTTCTG TATCTTTCAG AAAAATAAGG 540

TATACTGTAT GTAAACGATT TCAAAGGAGT CCAGTTATGG CAAAAACATT TTTTATTCCA 600

AATAAACAGA GCATTTTAGG AGAACAAGAG ATTTTGAATG CCAAGTCGAT CTTGGCTATG 660

ATGTAGTCTA TCTCCGTCAG CCTCTTAATC GTCTCGAGTA TATTGAGTGT GCGATAGTGG 720

GGCAATCACA ATTTCTTTTT AAGGTCAGTT ATGCTGATGG TCAAAAGGCT TACCGTGTCG 780

ATCTTCCTGA CCTACTAACA AAGACAGACT GGCAGATTAT CAAGTCATTT TTAGATGTTT 840

156

SUBSTTTUTE SHEET (RULE 26) TGCTTGCTTA TACAGGGACT GATATTGAAG GGCTAGATGG TTTTGATTTT GAAGCTTATT 900

TCCAAGCAAG TATTCAAGCC TATCTAGCAG ACCCTGTAGC TCGTTTTACG ATTTGCCAAC 960

GAATTTTTAA TCCTATTTTC TTTAGTCGTG AGAACTTGAA AAGCTTTTTA GAGGCAGATG 1020

GCTTGGCTCA GTTTGAAGCG CGTGTGCGTG CGGTTCAAGA GACAGATGCC TACTTTGCGA 1080

GAGTTTCCTT CTATCAGGAT GGAGAAGGAA AAGTGCATGG CGTTTACCAT CTAGCTCAAG 1140

GAGTCAAGAC AGTTTTACCG AGAGAACCGT TTGTTCCTGC AGCCTATATT GAGCGAATTG 1200

GTGGATAAGG AAGTCCAGTG GGAGATTGAC TTGGTTCAAA TCACAGGAGA CGGCTCTAAA 1260

CCAGAAGACT ATGAATCCAT AGCTCGCTTG GACTATGCAA AATTCTTAGA GGTATTACCC 1320

CCATCTTTTT ACCACCAACT AGACGCCAAT CAAATAGAAA TACAACCCAT CCTAGGACAA 1380

GATTTTAAAA CATTAGCACA AGAAAAGTAA AGCAGAAGCA GGTCAATCGA CTTGCTTTTT 1440

TGACATAGAA AAAATCCTGC CAAGGATGAC AGGATTGCTA CTCAATGAAA ATCAAAGAGC 1500

AAACTAGGAA GCTAGCCGCA GGCTGTACTT GAGTACGGTA AGGCGAAGCT GACGTGGTTT 1560

GAATTTGATT TTCGAAGAGT ATGAATTTTA AAGAAAGGCC AAGATACGAA GAT ATCTCC 1620

AATCAGTGCC ACTTCAGCTT CCAAGAAGAA GAAGATTATA ACTCCCGTTC CCCAAGGACA 1680

GA 1682

(2) INFORMATION FOR SEQ ID NO: 46:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 3041 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46:

ATCGAATTAA AAATGAGGTA TTCAGGCTTG TGATTTTCTA TGGAAGTTAA TAGTGATTGC 60

CTCTAATGCT TACAAGTGAT ATTAAAAATA GAGGACCTAG TGATGTCAAT CATTTCAACT 120

GATTTAACCC CTTTTCAAAT AGATGATACA TTGAAAGCAG CCTTGCGAGA AGATGTTCAT 180

TCCGAAGATT ACAGTACCAA TGCCATTTTT GATCATCATG GCCAAGCCAA GGTGTCGCTT 240

TTTGCCAAGG AAGCTGGTGT TTTAGCGGGG CTAACCGTTT TTCAAAGGGT TTTTACCCTA 300

TTTGATGCCG AGGTGACCTT CCAGAATCCT CATCAATTTA AGGATGGGGA TCGTTTGACT 360

AGTGGCGATT TGGTTTTAGA AATCATAGGC TCGGTGAGAA GTCTCTTAAC ATGTGAACGC 420

GTTGCCTTGA ATTTTTTACA ACATTTATCA GGGATCGCTT CGATGACAGC TGCTTATGTA 480

GAAGCCTTAG GCGATGATTG CATTAAGGTA TTTGATACTC GAAAAACTAC TCCTAATTTA 540

CGTCTTTTTG AGAAATATGC CGTGAGAGTT GGCGGTGGCT ATAATCATCG CTTTAATTTA 600

TCAGATGCTA TCCTGCTAAA AGACAATCAC ATTGCGGCAG TAGGTAGTGT TCAAAGGGCA 660

ATTGCTCAAG CGCGTGCCTA TGCTCCTTTT GTGAAAATGG TCGAGGTGGA AGTGGAAAGC 720

CTTGCTGCTG CCGAAGAAGC TGCGGCGGCG GGTGCTGATA TTATCATGTT GGATAATATG 780

TCATTGGAAC AGATTGAACA GGCCATTACC CTAATTGCAG GACGTTCTCG GATTGAATGT 840

TCTGGAAATA TTGATATGAC CACTATTAGC CGTTTTCGTG GTTTAGCGAT TGATTACGTC 900

TCCAGTGGTA GTTTAACCCA TAGTGCTAAG AGTCTTGATT TTTCCATGAA GGGTTTAACC 960 TACCTTGATG TCTAAGTTGT AAAATAAACT AACTTTTTAA AGGATGTCTT TCCTCTAGAA 1020

CGAGTTTTAT GTCAGATAGT TTAAACGCCT CTTCAAATAT AGTAAAATGA ACCAAAAATA 1080

GTACACAATG TGGTATAATC TTCTTATGGC ATATTCAATA GATTTTCGTA AAAAAGTTCT 1140

TTCTTATTGT GAGCGAACAG GTAGTA AAC AGAAGCATCA CACGTTTTCC AAATCTCACG 1200

TAATACCATT TATGGCTGGT TAAAGCTAAA AGAGAAAACA GGAGAGCTAA ACCACCAAGT 1260

AAAAGGAACA AAACCAAGAA AAGTTGATAG AGATAGACTT AAAAACTATC TTACTGACAA 1320

TCCAGACGCT TATTTGACTG AAATAGCTTC TGAATTTGGC TGTCATCCAA CTACCATCCA 1380

CTATGCGCTC AAAGCTATGG GCTACACTCG AAAAAAGGAC CACACCTACT ATGAACAAGA 1440

CCCAGAAAAA GTAGCCTTAT TTCTTAAAAA TTTTAATAGT TTAAAGCACC TAGCACCTGT 1500

TTAGATTGAT GAAACAGGAT TCGATACTTA TTTTTATCGA GAATATGGTC GCTCATTAAA 1560

AGGTCAGTTA ATAAGAGGTA AAGTATCTGG AAGAAGATAT CAGAGGATTT CTTTGGTTGC 1620

AGGTCTAACA AATGGTGAGT TAATCGCTCC AATGACTTAC GAAGAGACGA TGACGAGCGA 1680

CTTTTTTGAA GCATGGTTTC AGAAGTTTCT CTTACCAACA TTAACCACAC CATCGGTTAT 1740

TATTATGGAT AATGCAAGAT TCCATAGAAT GGGTAAGTTA GAACTTTTAT GCGAGGAGTT 1800

TGGGCATAAA CTTTTACCTC TTCCTCCCTA CTCGCCTGAG TACAATCTTA TTGAGAAAAC 1860

ATGGGCTCAT ATCAAAAAGC ACCTCAAAAA GGTATTACCA AGTTGCAATA CCTTTTATGA 1920

GGCTCTTTTG TCCTGCTCTT GTTTCAATTG ACTATAGTTC ACGGATACAG TTGGGAAAGA 1980

AGTTAAATGT AGTTGGATTT CCACTAAAGG TTGATGAGTA AGTTTTTGTA TCTGAACCTG 2040

ATTGGCCGCA AGCAGCTAAA AGCAAAGCAG ATGCAAAAGT CAGACCTGCA CCAAGGACAC 2100

GCTTCTTTAT GTTCATCTTC TTTCTCCTTA ATAGTGGGAA TTTGTAAAGT TAATTGAATT 2160

TCAAGAATGA AGGTTTTATA AACTTTGGTT ATAAAAAACA AAGGATTTCT GTCTTTTATA 2220

CAGTCCTCCC CTTGTTTTTA TACGATTTCA ATTTTAAATT TTTCTGCAAA AAATATTTAT 2280

AGTAATTCCA CACAGAAAGC ATCCCATGGA ACTAAGATTT GTTTTTCAAA GACTTCTTGA 2340

GCTAGGGTGT TTTCAATCAA GACAGATTTG ACTTTTCCTT CTACTGTCAA GTCTTGCTCT 2400

TCATTGGACA AGTTAGCCAC AACTAGGAAG CGACGGTCGC CATCCTTACG TATATAAGCA 2460

AAGACCTTAT CAGCCGTATC AAGCAATTCA AAGTCAGCTC GAATTAGCCA ACTATTCTCC 2520

TTGCGAATTT GGACCAGTTT CTGATAGGTA TAGAAAATAG AATCTGGATT TGCCAGCGCT 2580

TCTTGGACGT TGATCATCTC GTAATTTGGA TTAACTGCCA ACCAAGGTTG ACCTGTTGAG 2640

AAACCAGCGT TTTTGCTCTC GTCCCATTGC ATAGGGGTAC GGGCATTGTC ACGTCCAATA 2700

ACACGGATAC TGTCCATGAT TTCTTGCATC GGAACACCTT TTTCAAGAGC CTCACGCGCA 2760

TAGTTGAGAG ATTCAATATC TTCTACTTGA TCCAGTGTTT CAAACGGATA GTTGGTCATC 2820

CCAATCTCCT CACCTTGGTA GATATAAGGA GTTCCTCTCA TAAGATGAAG CAAGATTGCA 2880

AAGGCTTTGG CAGATTTTTC GCGGTATTCT TGGTCATTTC CCCAGATTGA GACAATACGA 2940

GGGAGGTCAT GGTTGTTCCA GAAGAGGGAA TTCCAGCCGT CCTCAACTCC TAACTCTGTC 3000

TGCCATTTGT TGAAGATTTC TTTTAACTTA GCGATATTCA G 3041

(2) INFORMATION FOR SEQ ID NO: 47:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 4694 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

158

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 47:

TTAATTTAAA TTCTTAAAAT TTTTTCATAA TAATCTCCCT ATAAAAATAA AGTCGCCCAA 60

TCAGGCGGCT TATTTTTTTG AAAAATGGGC TTGGTGCCTG AGAATAAATA GCTTAGTGAT 120

AGAAGAAAAT GGGGAAATAT GGTATAATGA AACGATAGAT TTTTGAATAG GAATAAGATC 180

ATGTTTGGAT TTTTTAAGAA AGATAAAGGC TGTGGAAGTA GAGGTTCCGA CACAGGTTCC 240

TGCTCATATC GGCATCATCA TGGATGGCAA TGGCCGTTGG GCTAAAAAAC GTATGCAACC 300

GCGAGTTTTT GGACATAAGG CGGGCATGGA AGCATTGCAA ACCGTGACCA AGGCAGCCAA 360

CAAACTGGGC GTCAAGGTTA TTACGGTCTA TGCTTTTTCT ACGGAAAACT GGACCCGTCC 420

AGATCAGGAA GTCAAGTTTA TCATGAACTT GCCAGTAGAG TTTTATGATA ATTATGTCCC 480

GGAACTACAT GCGAATAATG TTAAGATTCA AATGATTGGG GAGACAGACC GCCTGCCTAA 540

GCAAACCTTC GAAGCTTTAA CCAAGGCTGA GGAATTGACT AAGAACAACA CAGGATTGAT 600

TCTTAATTTT GCTCTTAACT ATGGTGGACG TGCTGAGATT ACACAGGCGC TTAAGTTGAT 660

TTCCCAGGAT GTTTTAGATG CCAAAATCAA CCCAGGTGAC ATCACAGAGG AATTGATTGG 720

TAACTATCTC TTTACCCAGC ATTTGCCTAA GGACTTACGA GACCCAGACT TGATTATCCG 780

TACTAGTGGA GAATTGCGTT TGAGCAATTT CCTTCCATGG CAGGGAGCCT ATAGTGAGCT 840

TTATTTTACG GACACCTTAT GGCCTGATTT TGACGAAGCG GCCTTGCAGG AAGCTATTCT 900

TGCCTATAAT CGTCGCCATC GCCGATTTGG AGGAGTTTAG GAGGAAATAT GACCCAGGAT 960

TTACAGAAAA GAACCTTGTT ATGCAGGGAT TGCCCTGACT ATTTTCCTAC CAATTTTAAT 1020

GATTGGGGGC TCTTGCTTCA GATAGCAATC GGAATCATAN CCATGCTAGC CATGCATGAA 1080

CTTTTGAAGA TGAGAGGTCT AGAGACCATG ACGATGGAGG CCTCTTGACC CTCTTTGCAC 1140

NTTNGTATTG ACCATTCCCC TGGAATCGAA TTACCTGACT TTTTTGCCAG TTGATGGGAA 1200

TGTGGTTGCC TATAGTGTTT TGATTTCAAT CATGTTAGGA ACGACCGTTT TTAGCAAGTC 1260

TTATACGATT GAGGATGCGG TTTTCCCTCT TGCTATGAGC TTCTACGTGG GCTTTGGATT 1320

TAATGCTTTA CTAGATGCTC GTGTTGCAGG TTTGGACAAG GCTCTCTTAG CCTTGTGTAT 1380

CGTCTGGGCG ACAGACAGTG GTGCCTATCT TGTTGGGATG AACTATGGGA AACGAAAGTT 1440

AGCACCAAGG GTATCGCCTA ATAAAACCCT TGAGGGTGCC TTGGGTGGTA TTTTAGGAGC 1500

AATTTTAGTA ACCATTATCT TTATGATAGT TGACAGTACA GTTGCTCTTC CATATGGAAT 1560

TTACAAGATG TCAGTCTTTG CTATTTTCTT TAGCATTGCT GGACAATTTG GTGATTTACT 1620

AGAAAGTTCG ATCAAACGTC ATTTTGGTGT TAAGGATTCT GGGAAATTTA TCCCTGGACA 1680

TGGTGGTGTT TTGGATCGTT TCGATAGTAT GTTGCTTGTA TTTCCAATCA TGCACTTATT 1740

TGGACTCTTT TAATCAAAAG ACGGAGGAAA CGCTATGCTC GGAATTTTAA CCTTTATTCT 1800

GGTTTTTGGG ATTATTGTAG TGGTGCACGA GTTCGGGCAC TTCTACTTTG CCAAGAAATC 1860

AGGGATTTTA GTACGTGAAT TTGCCATCGG TATGGGACCT AAAATCTTTG CTCACATTGG 1920

CAAGGATGGA ACGGCCTATA CCATTCGAAT CTTGCCTCTG GGTGGCTATG TCCGCATGGC 1980

CGGTTGGGGT GATGATACAA CTGAAATCAA GACAGGAACG CCTGTTAGTT TGACACTTGC 2040

TGATGATGGT AAGGTTAAAC GCATCAATCT CTCAGGTAAA AAATTGGATC AAACAGCCCT 2100

CCCTATGCAG GTGACCCAGT TTGATTTTGA AGACAAGCTC TTTATCAAAG GATTGGTTCT 2160

GGAAGAAGAA AAAACATTTG CAGTGGATCA CGATGCAACG GTTGTGGAAG CAGATGGTAC 2220

TGAGGTTCGG ATTGCACCTT TAGATGTTCA ATATCAAAAT GCGACTTTAT CTGGGGCAAA 2280

CTGATTACCA ATTTTGCAGG TCCTATGAAC AATTTTATCT TAGGTGTTGT TGTTTTTTGG 2340

159

SUBSTTTUTE SHEET (RULE 26) GTTTTAATCT TTATGCAGGG TGGTGTCAGA GATGTTGATA CCAATCAGTT CCATATCATG 2400

CCCCAAGGTG CCTTGGCCAA GGTAGGAGTA CCAGAAACGG CACAAATTAC CAAGATTGGC 2460

TCACATGAGG TTAGCAACTG GGAAAGCTTG ATCCAAGCTG TGGAAACAGA AACCAAAGAT 2520

AAGACGGCAC CGACTTTGGA TGTGACTATT TCTGAAAAGG GGAGTGACAA ACAAGTCACT 2580

GTTACACCCG AAGATAGTCA AGGTCGTTAC CTTCTAGGTG TTCAACCGGG GGTTAAGTCA 2640

GATTTTCTAT CCATGTTTGT AGGTGGTTTT ACAACTGCTG CTGACTCAGC TCTCCGAATT 2700

CTCTCAGCTC TGAAAAATCT GATTTTCCAA CCGGATTTGA ACAAGTTGGG TGGACCTGTT 2760

GCTATCTTTA AGGCAAGTAG TGATGCTGCT AAAAATGGAA TTGAGAATAT TCTTGTACTT 2820

CTTGGCAATG ATTTCCATCA ATATTGGGAT TTTTAATCTT ATTCCGATTC CAGCCTTGGA 2880

TGGTGGTAAG ATTGTGCTCA ATATCCTAGA AGCCATCCGC CGCAAACCAT TGAAACAAGA 2940

AATTGAAACC TATGTCACCT TGGCCGGAGT GGTCATCATG GTTGTCTTGA TGATTGCTGT 3000

GACTTGGAAT GACATTATGC GACTCTTTTT TAGATAATCG AGGAATATTA TGAAACAAAG 3060

TAAAATGCCT ATCCCAACGC TTCGCGAAAT GCCAAGCGAT GCTCAAGTTA TCAGCCATGC 3120

TCTTATGTTG CGTGCTGGTT ATGTTCGCCA AGTTTCAGCA GGTGTTTATT CTTATCTACC 3180

ACTTGCCAAC CGTGTGATTG AAAAAGCTAA AAACATCATG CGCCAAGAAT TCGAAAAGAT 3240

TGGTGCTGTT GAGATGTTGG CTCCAGCCCT TCTTAGTGCA GAATTGTGGC GTGAATCAGG 3300

TCGTTACGAA ACCTATGGTG AAGACCTTTA CAAACTGAAA AACCGTGAAA AATCAGACTT 3360

TATCTTAGGT CCAACTCACG AAGAAACCTT TACAGCTATT GTCCGTGATT CTGTTAAATC 3420

TTACAAGCAA TTGCCACTCA ACCTTTATCA AATTCAGCCC AAGTATCGTG ATGAAAAACG 3480

CCCACGTAAT GGACTTCTTC GTACACGTGA GTTTATCATG AAGGATGCTT A AGTTTCCA 3540

CGCTAACTAT GATAGTTTGG ATAGTGTTTA TGATGAGTAC AAAGCAGCCT ATGAGCGTAT 3600

TTTCACTCGT AGTGGTTTAG ACTTCAAGGC TATTATTGGT GACGGTGGAG CCATGGGTGG 3660

TAAGGATAGC CAAGAATTTA TGGCCATTAC ATCTGCTCGT ACAGACCTTG ACCGCTGGGT 3720

TGTCTTGGAC AAGTCAGTTG CCTCATTTGA CGAAATTCCT GCAGAAGTGC AAGAAGAAAT 3780

CAAGGCAGAA TTGCTCAAAT GGATAGTCTC TGGTGAAGAT ACCATTGCTT ACTCAAGTGA 3840

GTCTAGCTAT GCAGCTAACT TAGAAATGGC AACAAACGAG TACAAACCAA GCAACCGTGT 3900

TGTCGCTGAA GAAGAAGTTA CTCGTGTTGA AACGCCAGAT GTTAAATCAA TTGATGAAGT 3960

TGCAGCCTTC CTCAATGTTC CAGAAGAACA AACGATTAAA ACCCTCTTCT ACATTGCAGA 4020

TGGTGAGCTT GTTGCAGCCC TTCTAGTTGG AAATGACCAA CTCAACGAAG TCAAGTTGAA 4080

AAATCACTTG GGAGCAAATT TCTTTGACGT TGCTAGCGAA GAAGAAGTGG CGAATGTTGT 4140

TCAAGCAGGA TTTGGTTCAC TTGGACCAGT TGGTTTGCCA GAGAATATTA AAATTATTGC 4200

AGATCGTAAG GTGCAAGATG TTCGCAATGC AGTTGTCGGT GCTAACGAAG ATGGCTACCA 4260

CTTGACTGGT GTGAACCCAG GCCGTGATTT TACTGCAGAA TATGTGGATA TCCGTGAAGT 4320

TCGTGAGGGT GAAATTTCCC CAGATGGACA AGGTGTCCTT AACTTTGCGC GTGGTATTGA 4380

GATCGGTCAT ATTTTCAAAC TCGGAACTCG CTATTCAGCA AGCATGGGAG CAGATGTCTT 4440

GGATGAAAAT GGTCGTGCTG TGCCAATCAT CATGGGATGT TACGGTATCG GTGTCAGCCG 4500

TCTTCTTTCA GCAGTGATGG AGCAACACGC TCGCCTCTTT GTTAACAAAA CGCCAAAAGG 4560

TGAATACCGT TACGCTTGGG GAATCAATTT CCCTAAAGAA TTGGCACCAT TTGATGTGCA 4620

TTTGATTACT GTTAATGTCA AGGATGAAGA AGCGCAAGCC TTGACAGAAA AACTTGAAGC 4680

AAGCTTGATG GGAG 4694

(2) INFORMATION FOR SEQ ID NO: 48:

160

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1352 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 48:

CTCGTAAGTT CGGAAGCTAT CTACACAAGA AATTAACCGC TGCCTAAAGG AGAAGCCATG 60

TCAACATATA ACTGGGATGA GAAGCATATC CTTACCTTTC CTGAAGAAAA AGTAGCCCTT 120

TCTACTAAGG ATGTCCATGT TTACTATGGT AAAAATGAAT CCATTAAGGG GATTGATATG 180

CAATTTGAAA GAAATAAAAT TACAGCTTTG ATTGGTCCGT CGGGATCGGG GAAATCTACC 240

TACTTACGCA GTCTCAATCG CATGAATGAT ACCATTGATA TTGCTAAAGT AACTGGGCAG 300

ATTCTCTATC GTGGAATTGA TGTCAACCGT CCAGAAATCA ACGTTTATGA AATGCGTAAA 360

CACATTGGAA TGGTTTTTCA ACGCCCCAAT CCATTTGCTA AATCGAATTT ACCGTAATAT 420

TACCTTTGCG CATGAACGTG CTGGAGTTAA GGATAAGCAA GTCCTAGATG AAATCGTAGA 480

AACCTCCCTT AGTCAGGCTG CCCTTTGGGA TCAGGTTAAA GACGATCTCC ACAAGTCAGC 540

CTTGACCTTA TCAGGTGGTC AGCAACAACG TCTCTGTATC GCTCGTGCCA TCTCTGTTAA 600

GCCAGATATC CTCTTAATGG ATGAGCCAGC CTCAGCCTTG GATCCGATTG CGACCATGCA 660

ACTAGAAGAG ACCATGTTTG AGCTCAAGAA AAACTTTACC ATCATCATTG TAACGCATAA 720

TATGCAGCAG GCTGCTCGTG CAAGTGACTA TACAGGCTTC TTTTACTTGG GTGATTTGAT 780

TGAGTATGAC AAGACTGCAA CTATTTTCCA AAATGCCAAG CTACAGTCCA CCAATGACTA 840

TGTATCTGGT CACTTTGGTT AGAAAGGAAA CCGTATGACA GATGCGATTT TACAGGTATC 900

AGACCTGTCC GTTTATTATA ATAAAAAGAA GGCTTTGAAT AGTGTTTCCC TATCTTTCCA 960

ACCTAAGGAA ATTACAGCCT TGATTGGTCC ATCTGGATCA GGGAAGTCAA CCCTCCTCAA 1020

GTCTCTCAAC CGCATGGGAG ATCTCAATCC AGAGGTGACC ACAACTGGAT CCGTGGTGTA 1080

CAATGGTCAC AACATCTACA GTCCGCGTAC AGATACGGTT GAATTACGTA AGGAAATCGG 1140

AATGGTTTTC CAACAACCTA ATCCTTTCCC TATGACTATC TATGAGAATG TTGTCTACGG 1200

GCTTCGTATC AATGGAATTA AGGATAAGCA GGTTCTGGAT GAAGCCGTAG AAAAAGCCTT 1260

GCAAGGTGCC TCTATCTGGG ATGAGGTCAA GGATCGTCTA TATGATTCAG CTATTGGATT 1320

GTCAGGTGGT CAACAGCAGC GTGTCTGCGT GG 1352

(2) INFORMATION FOR SEQ ID NO: 49:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2258 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 49:

161

SUBSTTTUTE SHEET (RULE 26) AACTTCGACC GTGATAAACA AGCTGAGCTT TGACATACTT GTAGCCAACC TAAAAGCCGT 60

TCTTCAAGGC CTCAAACCAG CTGCAACTCA TTCAGGAAGC CTGGATGAAA ATGAAGTGGC 120

TGCCAATGTT GAAACCAGAC CAGAACTCAT CACAAGAACT GAAGAAATTC CATTTGAAGT 180

TATCAAGAAA GAAAATCCTA ATCCCAGCTG GTCAGGAAAT ATTATCACAG CAGGAGTCAA 240

AGGTGAACGA ACTCATTACA TCTCTGTACT CACTGAAAAT GGAAAAACAA CAGAAACAGT 300

CCTTGATAGC CAGGTAACCA AAGAAGTTAT AAACCAAGTG GTTGAAGTTG GCGCTCCTGT 360

AACTCACAAG GGTGATGAAA GTGGTCTTGC ACCAACTACT GAGGTAAAAC CTAGACTGGA 420

TATCCAAGAA GAAGAAATTC CATTTACCAC AGTGACTCGT GAAAATCCAC TCTTACTCAA 480

AGGAAAAACA CAAGTCATTA CTAAGGGTGT CAATGGACAT CGTAGCAACT TCTACTCTGT 540

GAGCACTTCT GCCGATGGTA AGGAAGTGAA AACACTTGTA AATAGTGTCG TAGCACAGGA 600

AGCCGTTACT CAAATAGTCG AAGTCGGAAC TATGGTAACA CATGTAGGCG ATGAAAACGG 660

ACAAGCCGCT ATTGCTGAAG AAAAACCAAA ACTAGAAATC CTAAGCCAAC CAGCTCCTGC 720

TGAGGAAAGC AAAGCTCTTC CTCAAGATCC AGCTCCTGTG GTAATAGAGA AAAAACTTCC 780

TGAAACAGGA ACTCACGATT CTGCAGGGAC TAGTAGTCGC AGGACTCATG GCCACACTAG 840

CAGCCTATGG ACTCACTAAA AGAAAAGAAG ACTAAGTCTT TTCGATAAAA AATAAACAGC 900

GAGATTGAAG CTCGCTGTTT ATTTTTTAAT TAATCACCTA GTCCAAGACG TTCAAAGATA 960

TCATCCACTC GTTTGGTGTA A AAACTGGG TTGAAGATTT CATCGATTTC TTCTTGTGTG 1020

AGACGTGATG TTACTTCTGA ATCTGCCTCA AGAAGTGGTT TAAAGTCTAC TTGGTTGTCC 1080

CAAGAGTAGG CTGTTTTTGG TTGCACCAAG TCATAGGCTT GCTCACGGGT CATGCCTTTT 1140

TCAATCAATG TCAACATAGC CCGTTGGCTA AAGATAAGAC CAAAAGTCGA GTTCATGTTT 1200

CGGATCATAT TTTCTGGGAA GACTGTCAAG TTCTTGACGA TATTTCCAAA ACGGTTGAGC 1260

ATGTAGTCAA TCAAAATGGT CGTATCTGGT GTGATGATAC GCTCAGCTGA TGAGTGAGAA 1320

ATATCGCGTT CGTGCCAGAG AGCGACGTTT TCATAAGCCG TAATCATGTG ACCACGAATG 1380

ACACGCGCCA GACCAGTCAT ATTTTCAGAA CCGATTGGGT TGCGTTTGTG AGGCATTGCT 1440

GAAGACCCTT TTTGCCCTTT AGCAAAGAAC TCTTCTACTT CGCGTTGCTC AGATTTTTGT 1500

AGACCACGAA TCTCAGTCGC CATACGTTCG ATTGAAGTCG CAATGCTGGC AAGAACCGCA 1560

AAGTACTCAG CGTGAAGGTC ACGAGGAAGG ACTTGTGTTA AAGATTCCTT GGGCACGGAT 1620

GCCAAGATTT ATCGCAGACA TACTCCTCTA CAAATGGTGG GATATTGGCA AAGTTCCCAA 1680

CCGCACCAGA AATCTTACCA GCTTCTACAC CAGCAGCCGC ATGCTCGAAG CGCTCGATAT 1740

TGCGTTTCAT TTCGCTGTAC CAAGTTGCTA ATTTAAGACC AAAGGTTGTC GGCTCAGCGT 1800

GCACACCATG AGTACGCCCC ATCATGATGG TGAACTTGTG CTCCTTGGCC TTGTCAGCGA 1860

TGATATTAGT GAAGTTTTCA AGGTCACGAC GGATGATGTC GTTGGCCTGC TTGTAGAGGT 1920

AACCATAAGC AGTATCCACC ACGTCGGTAG AAGTTAACCC ATAGTGAACC CACTTGCGCT 1980

CTTCACCAAG AGTCTCAGAA ACCGCACGCG TGAAAGCCAC CACATCGTGG CGCGTCTCCT 2040

GCTCAATTTC CAAAATACGG TCGATGTCAA AGTCCGCCTT CTTGCGAATC AAAGCCACAT 2100

CTTCCTTAGG GATTTCCCCC AACTCAGCCC ATGCCTCGTC AGAGAGGATT TCCACCTCAA 2160

GCCAAGCACG GTATTTATTT TCTTCACTCC AAATATTCGC CATCTCAGGG CGAGAGTAAC 2220

GGTTGATCAT GTGTTAATTT TTCCTTTCTT CTTAAGAT 2258

(2) INFORMATION FOR SEQ ID NO: 50:

(i) SEQUENCE CHARACTERISTICS:

162

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 4392 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50:

CCCTTTTGCC TCTCCCTTTG GTGCAGATTC TTTTGGGAAT TGTGATTGGT CTCTTTTTAC 60

CCAATACTGA CTTTCATCTT AATACGGAGT TGTTTTTGGC CTGGTTATCG GACCCTTGCT 120

TTTCCGAGAG GCTGAAGAAG CAGATGTTAC GGCTATTTTA AAACACTGGC GAATCATTGT 180

TTATCTCATA TTTCCAGTGA TTTTTATCTC GACCCTGAGT TTGGGTGGCT TGGCCCATCT 240

TCTTTGGTTC AGCCTTCCCT TGGCAGCTTG CTTGGCTGTT GGGGCAGCCC TTGGTCCTAC 300

GGACTTGGTG GCCTTTGCCT CTCTTTCGGA GCGTTTTAGC TTTCCTAAGC GCGTGTCCAA 360

TATTCTTAAG GGCGAAGGAC TCTTGAATGA TGCTTCTGGT TTGGTGGCTT TTCAGGTAGC 420

TTTGACAGCT TGGACAACTG GAGCTTTTTC TCTGGGGCAA GCTAGCAGTT CGCTCATCTT 480

TTCAATCCTA GGCGGTTTTT TAATTGGATT TTTAACAGCC ATGACCAACC GCTTCCTCCA 540

TACCTTCTTG CTAAGTGTGC GCGCAACGGA TATTGCCAGT GAACTTTTAT TAGAATTCGA 600

GTTTGCCTCT AGTGACCTTC TTTCTGGCAG AAGAAGTCCA TGTTTCAGGG ATTATTGCCG 660

TCGTAGTTGA TCGAATTTTA AAGGCAAGTC GCTTCAAGAA AATCACGCTC CTCGAAGCCC 720

AAGTGGATAC GGTGACCGAG ACGGTCTGGC ATACAGTGAC CTTTATGCTC AACGGTTCTG 780

TCTTTGTGAT TTTAGGGATG GAGTTGGAAA TGATAGCAGA ACCTATCTTG ACCAATCCAA 840

TCTATAATCC TCTACTTTTA TTGCTATCTC TCATCGCCCT TACCTTTGTC CTCTTTGTCA 900

TTCGTTTTAT TATGATCTAT GGCTATTATG CCTATAGAAC CCGACGCCTA AAGAAAAAGC 960

TAAATAAGTA TATGAAGGAC ATGTTTCTCT TGACCTTTTC AGGTGTTAAG GGAACGGTGT 1020

CGATTGCTAC GATTCTCTTG ATACCAAGTA ATCTAGAACA GGAGTATCCT CTCTTGCTTT 1080

TCCTTGTTGC AGGTGTGACG CTTGTCAGCT TTTTAACAGG TCTCTTGGTC TTGCCTCATC 1140

TTTCTGATGA AGAGGAAGAA AGCAAGGATT ATCTCATGCA TATCGCCATT TTGAATGAAG 1200

TAACGCTAGA GTTGGAAAAA GAGTTGGAAG ACACCAGAAA TAAACTTCCC CTCTATGCGG 1260

CTATTGACAA TTCGATCATG GACGTATTGA AAATCTCATT TTAAGCCAAG AAAACCAGGA 1320

TGATCAAGAA GACTGGGCTG CTTTGAAAAT CGAATTCTTA GTATTGAAAG TGATGGTTTG 1380

GAACAGGCCT ATGAAGAGGG GAACATTAGC AATCGTGCTT ACCGAGTTTA CCAACGTTAT 1440

CTGAAAAATA TAGAACAAGG AATCAATCGT AAACTTGCCT CAAGACTGAC CTATTATTTT 1500

CTTGTTTCCT TGAGGATTTT ACGTTTTCTT CTTCATGAAG TTTTTACTCT TGGAAAGACC 1560

TTCCGTAGCT GGAAGGACAA GGAGCAAAGC CGTCTCCGTG CTCTTGATTA TGACCAAATT 1620

GCAGAGCTCT ATCTTGCCAA TACAGAGATG ATTATTGAAA GTTTGGAAAA CCTGAAGGGA 1680

GTCTACAGAC GCTCTTTGAT TAGTTTTATG CAGGAGTCTC GTCTTCGAGA AACAGCTATT 1740

ATCAGCAGTG GTGCCTTTGT CGAACGGGTT ATCAATCGTG TCAAACCCAA CAATATCGAT 1800

GAAATGCTGA GAGGCTATTA TCTGGAGCGC AAGTTGATTT TCGAATACGA AGAAAAACGA 1860

TTGATTACGA CTAAGTATGC CAAGAAATTA CGACAAAATG TAAATAACTT AGAGAACTAT 1920

TCCTTGAAGG AAGCTGCCAA TACCCTGCCG TATGATATGG TGGAATTGGT AAGAAGAAAT 1980

TAGTTAATAC TCTTCGAAAA TCTCTTCAAA CCACGTCAGC GTCGCCTTGG ATTATATATG 2040

163

SUBSTTTUTE SHEET (RULE 26) TGACTGACTT CGTCAGTTTC ATCTACAACC TCAAAGCAGG GCTTTGAGCA ACCTGCGGCT 2100

AGCTTCCTAG TTTGCTCTTT GATTTTCATT GAGTATAAGA TTGTAAGTGA AGGAGTGTGA 2160

CATGAAAAAA TGGGGAAAGA GCCTGAACTA GTCCTGTCTA CTTTTACCCA ATCACACTTC 2220

CATTTGGTAC AGCTGGATCA ACTGTGAGAA GGGATCGAAT TTGCCATCAT GTTCAGCTGA 2280

GAGAATCATA CCCTGGCTGA CATATTTTTT CATCATTTTA CGTGGTTTGA GGTTAGCAAC 2340

GATTTGAACT TTCTTGCCGA CCAATTCTTG TTCATTTGGA TAGTATTTTG CAATTCCTGA 2400

AAGAATCTGA CGATCTTCTC CATCACCAGC ATCCAAGCGG AATTGAAGCA ACTTATCTGA 2460

ACCTTCTACT TTAGACACTT CTTTGACTTC TGCGACACGG ATTTCAACCT TGTCAAAGTC 2520

TTCAAACTTG ATTTCATCCT TGTTTAGTTT GAGCTCAACT TCGTCCGGAT TCCATTCTTT 2580

TTCGACTGCT GGTTTATTGC CTTCCATTTG TTCCTTGATA TAGGCGATTT CTTCTTCCAT 2640

ATTTAGACGT GGAAAGATAG GTGTTCCTTT GGCAACTACA GTCACATCTG CTGGGAAGTC 2700

AGCCAAACTC AAGTTTTCAA GACTAGAAAC TTCTTCCAAA CCAAGTTGAG TCAAAACTGC 2760

ACGACTAGTT TCCATCATAA ATGGTTCAAT CAAGTGAGCA ACTACACGAA TGCTGGCTGC 2820

CAAGTGGCTC ATGACACTTG CCAATTGGTC ACGAAGAGCT TCATCCTTGT CCAAGACCCA 2880

TGGTGCAGTC TCATCGATGT ATTTATTGGT ACGAGAGATC AGAGTCCAGA CTGCTTCAAG 2940

CGCACGTGGA TAGTCAACTG CTTCCATGTG TGTATGGAAG TCTGCGATTG ATTTTTCTGC 3000

AACCTCAGCA AGAACATGAT CAAATTCAGT CACACCTTCT ACATAGGCAG GGATTTGTCC 3060

ATCAAAGTAC TTATTAATCA TGGAAACCGT ACGGTTAAGG AGGTTCCCAA GGTCATTAGC 3120

CAATTCATAG TTGATACGAC CGACATAGTC TTCAGGAGTA AAGGTTCCGT CTGAACCAAC 3180

TGGAAGGTTA CGCATGAGGT AGTAACGAAG TGGATCTAGT CCATAACGCT CTACCAACAT 3240

TTCAGGGTAA ACGACATTCC CTTTTGACTT AGACATTTTT CCGTCTTTCA TGACAAACCA 3300

ACCATGGGCA ATCAAACGAT CAGGTAATTT AACATCCAAC ATCATAAGAA GGATTGGCCA 3360

GTAGATAGAG TGGAAGCGAA GGATGTCTTT TCCTACCATA TGGAAGACTG TTCCATTCCA 3420

GAACTTGTCA AAGTTACCAT GTTCGTCTTG AGCGTAGCCA AAAGCTGTCG CATAGTTAAG 3480

AAGGGCATCA ATCCAAACGT AGACAACGTG TTTTGGATTT GATGGGACAG GCACTCCCCA 3540

TGTAAAGGTT GTACGAGATA CCGCCAAATC TTCCAAACCT GGCTCGATGA AGTTGCGTAG 3600

CATTTCATTA AGACGACCAT CTGGCGTGAT AAATTCAGGA TGAGCTTTGA AAAATTCGAC 3660

CAAACGGTCT TGGTATTTGC TAAGGCGAAG GAAGTATGAT TCTTCAGAAA CCCATTCAAC 3720

CTCATGACCT GATGGAGCAA TACCACCAGT CACATTTCCA GCTTCATCAC GGAAAACTTC 3780

TGCCAGCTGG CTTTCTGTAA AGAATTCTTC GTCTGATACT GAATACCAAC CAGAGTATTC 3840

ACCCAAGTAG ATATCATCTT GAGCAAGTAA GCGTTCAAAG ACCTGTGCGA CAACTTTTTC 3900

ATGGTAGTCA TCGGTTGTAC GGATAAATTT ATCGTATGAG ATATCTAGTA ATTGCCAGAG 3960

TTCTTTAACT CCAACCGCCA TTCCATCAAC ATAGGCTTGA GGTGTAATAC CAGATTCGAA 4020

TTCCGCTTTC TGCTGGATTT TCTGACCATG TTCATCAAGA CCTGTCAGAT AAAATACATC 4080

GTAGCCCATC AGGCGTTTGT AACGTGCTAG GACATCACAT GCGATAGTTG TGTAGGCAGA 4140

ACCGATATGA AGTTTCCCAG ATGGATAGTA AATCGGCGTT GTAATATAAA AATTTTTTTC 4200

AGACATAATT TTTCCTTTCC AGGCAAATGA AACCTGTTTT TCTAACACTT CATTATATCA 4260

CATTTTTAAT GAATTTCGAT AGGGAAATCC ATACCAAAAC AAGATAGACG AGTGTCCATC 4320

TTGTTGATCT CATTCATAAC GAAGGGCTTC AATTGGATCA AGTTTCGATG CCTTGTTGGC 4380

TGGCAAGACT CC 4392

(2) INFORMATION FOR SEQ ID NO: 51:

164

SUBSTTWTΕ SHEET (RULE 26) ;i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1941 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51:

AATTAGTATT CTCAACCTTT TTATCTTGAT AGTTCAAGAT GGCATTCGTT GAATTGGTAA 60

CATAGTAACT ATCCACTCCC TTCAGTTTAG CTGCCTCTTG AACCCAGGAT TCTTGCGGTT 120

TTGGCGGTTC AACAGGAATT CTTTTTCTTT TCCAGAAACC GTAAAAGCTG ATTGTTTCTG 180

AGTAAAAGAC CCATCTTTAC TTTTTTTAGG AGAGAAAAAG ACGCTAATAT TTTTCTGAGA 240

TTTAGTCATA TCTTTATTGA CTTGACGAGA TAGGGAATCA CCCAAAGCCA TAATCACAAC 300

AACTGATGAA ACACCGATAA TAATCCCAAT CATAGTAAGC AAAGAACGCA TCTTGTGAGC 360

CATGATAGAT GAAAAGGCAA ATTTCAGATT CTGCATCTTA GTTTTCCTCC TTTCCTAACT 420

GAGCACTGTC AGACGAAATG ACCCCATCCC GAATGACAAT CTGACGTTTG GCATAGGCAG 480

CAATCTCAGG CTTCATGCGT TACCATGATA ATGGTTTTTC CTTCTTTATT CAAATCAACC 540

AATAATTGCA TAATTTGGTT ACCTGTTTTG GTATCCAAGG CTCCTGTCGG TTCATCCGCT 600

AGGATAATAG AAGGATTGTT TACCAAGGCA CGCGCAATGG CTACACGTTG CTTTTGACCA 660

CCAGATAATT CTGAAGGTAA ATGGTGACTA CGTTCTATCA ATTCAACCTT GTCTAAATAT 720

TCCTCAGCCA ACTTGCGACG TTTTGAAGAC GAAACTCCTG CGTAAATCAA GGGCAATTCT 780

ACATTTTGCA GAGCATTGAG CTTCGATAGA AGAAAGAACT GCTGAAAGAC AAAACCGATT 840

TGTTGGTTAC GGACCTTAGC TAGTTGTTTT TCACCAAGCC CAGCCACTTC TTGACCTTCA 900

AGATAATATT CTCCACTGGT TGGTGTATCC AACATGCCAA TCGTATTCAT CAGAGTGGAC 960

TTACCAGACC CAGATGGTCC CATGATGGCT ACAAATTCAC CCTCATTCAC TTCTAGATTG 1020

ATATTTTTGA GAACCTGCAG TTCTTGGTCA CCATTACGGT AACTTCTGAA GA ATTTTTT 1080

AGACTAATTA GTTGCTTCAT CAGCCTTCAC CTCTTTTCCT TCTTCCAAGG AAGATGTTGG 1140

ATTACTGATG ACCTTAGCAC CGTTCGTTAA ACCAGAAGTG ATTTCTTGAT TTTCTGCGTC 1200

AGCATTTCCC AATGAAACCT CAACTTTTTT AGCCTTTTGT TGTTCATCCA CAATCCAGAC 1260

ATAATTTTTA CTATCATCCA TTACTAGACT GCTAACAGGA ACAAGAATAG CCTTAGTTTT 1320

GCTTTTAACC TCAATGTTGA CAGAAAAACC TTGTTTCAAA TCACCAACCT CGCCTGTCAC 1380

ATCAATAGTA TAAGGGTATT TAGAACCTGT ATTATTCCCG GCTGCTGGAC TAGCTGCTTC 1440

ACCATTGTTT TTAGGATAGT CAGAAATATA GGCTTAATTT CCCAGTCCAT TTTTTATCAG 1500

GATACACTTT AGAAGTAAAG CTTACTTCTT GACCTACAGA AAGGTTGGCT AGATTGTACT 1560

CAGACAATTC TCCCTTGACT TGTAAATTTT CATTGCTGAC AATATGAACC ATAACTTGAC 1620

TCGCCCCTGT TGGAGATTTA GAAACATTGC TATTGACTTC GACTACAGTT CCCTCTAGGG 1680

TACTGAGAAC AGTTGTTGCA TCCAATTGAC TTTGAGCCTT GCTTAATTGC GCTGCAGCAT 1740

CTGCACGCGC ATCACGGGCA TCACCCAATT GAGCATCAAT AGAAGCAACA GAATTTCCAG 1800

CCACTGGAGT TGGGCTTTGC ACCGTTGCAT CTTCTCCTCC TACTGGCGCT GGTAACTGTG 1860

GAGCCTGAGC TGAAGCGGCT TCATTTCGTG CTTGATTGAG TTCATTGATA TGACGATCTG 1920

CCTTAGCTAC TGCTCGACTA G 1941

165

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 52:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1335 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:52:

ATCGAATTCC CTATTTTAAC ACTTTCTTTT CTAAAACAGT CTATATTTTA TTTCAAACTG 60

TATTATATTT TTGAAAAAAT AAAGTCCTTT TTTCTTTTTT TCAGAAAAAA GGGTATAATA 120

AAAGAAAATA AGCAGTAACA CTCAATGGAA ATCGAAAAAG CAAACTAGGA AGCTAGCCGC 180

AGATTGCTCA AAACACTGTT TTGAGGTTGC AGATAGAGCT GACGTGGTTT GAAGAGATTT 240

TCGAAGAGTA TAAAAAGGTG CTAGGCATGT TGATTTTTCC TTTGTTAAAT GATTTGTCAA 300

GAAAAATCAT CCATATTGGA CATGGATGCC TTTTTTGCTG CAGTGGAAAT CAGGGATAAT 360

CCTAAACTCA GAGGAAAACC TGTCATTATT GGAAGCGACC CTCGGCAAAC AGGTGGACGG 420

GGAGTCGTTT CTACCTGTAG TTATGAGGCA AGAGCTTTTG GTGTCCATTC TGCCATGAGT 480

TCCAAGGAAG CTTATGAACG TTGTCCCCAG GCTGTCTTTA TCTCAGGGAA TTCGATGAGA 540

AATACAAGTC TGTGGGACTC CAGATTCGAG CTATTTTTAA GCGCTATACA GATTTGATTG 600

AACCCATGAG CATTGACGAA GCCTATTTGG ATGTGACAGA AAATAAACTC GGTATCAAGT 660

CAGCGGTCAA AATTGCTCGC CTCATTCAAA AAGATATCTG GCAAGAACTC CATCTAACTG 720

CTTCCGCAGG CGTTTCTTAC AACAAATTCT TAGCTAAAAT GGCGAGTGAT TATCAAAAAC 780

CACATGGTTT GACAGTGATT CTACCTGAAC AGGCTGAGGA TTTTCTCAAA CAAATGGATA 840

TTTCCAAATT TCATGGAGTA GGAAAAAAGA CAGTAGAACG TCTTCATCAA ATGGGCGTTT 900

TTACTGGTGC TGATTTACTT GAAGTTCCTG AGGTAACCCT AATAGACCGT TTTGGTAGAC 960

TAGGCTATGA TCTGTATCGA AAGGCTCGTG GCATTCACAA CTCTCCAGTC AAATCCAATC 1020

ACATCCGTAA ATCAATCGGC AAGGAGAAAA CCTACGGGAA GATTCTCCGT GCTGAGGAAG 1080

ATATCAAAAA AGAGAGCTGA CTCTTCTATC AGAAAAAGTC GCTCTCAATC TACATCAACA 1140

AGAAAAAGCT GGAAAAATTG TCATTTTGAA AATCCGCTAC GAGGACTTTT CAACTCTTAC 1200

CAAACGAAAA AGTATTGCTC AAAAAACACA AGATGCTAGT CAGATAAGCC AAATAGCCCT 1260

GCAACTCTAT GAAGAATTAA GTGAGAAAGA AAGAGGTGTC CGCCTATTGG GGATTACCAT 1320

GACTGGATTT TAAAG 1335

(2) INFORMATION FOR SEQ ID NO: 53:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1796 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

166

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 53:

TCCAAGCTAG CTATTTCGTG GAAGGGGCTT CGGTTGGCAG AACCTGGTGA ATTTACCCAA 60

ACGTGCTTTT TTAAACGGTC GCGTAGACTT GACACAGGCA GAGGCTGTGA TGGATATCAT 120

CCGTGCCAAG ACTGACAAGG CCATGAACAT TGCGGTCAAA CAATTAGACG GCTCCCTTTC 180

TGACCTCATT AACAATACCC GTCAAGAAAT CCTCAATACA CTTGCCCAAG TTGAGGTCAA 240

TATCGACTAT CCTGAATATG ATGATGTTGA GGAAGCTACT ACTGCCGTTG TCCGTGAGAA 300

GACTATGGAG TTTGAGCAAT TGCTAACCAA GCTCCTTAGG ACAGCACGTC GTGGTAAAAT 360

CCTTCGTGAA GGAATTTCAA CGGCTATCAT TGGACGTCCC AACGTTGGGA AATCAAGCCT 420

TCTCAACAAC CTCTTGCGTG AGGACAAGGC TATCGTAACC GATATCGCTG GGACAACACG 480

AGATGTCATC GAAGAGTACG TCAACATCAA TGGTGTTCCT CTAAAATTGA TTGACACAGC 540

TGGTATTCGT GAAACGGATG ATATCGTTGA ACAAATCGGT GTTGAGCGTT CGAAAAAAGC 600

CCTCAAGGAA GCCGACTTGG TTCTACTAGT GCTAAATGCC AGTGAACCAC TGACTGCGCA 660

AGACAGACAA CTTCTTGAAA TTAGCCAAGA TACCAATCGC AT ATTCTAC TTAATAAAAC 720

CGACCTGCCA GAAACGATTG AAACTTCGAA ACTACCTGAA GACGTTATCC GTATTTCAGT 780

CCTTAAAAAC CAAAACATCG ACAAGATTGA AGAGCGAATC AACAACCTCT TCTTTGAAAA 840

TGCTGGCTTG GTCGAGCAAG ATGCTACTTA CTTGTCAAAC GCCCGTCACA TTTCCCTGAT 900

TGAAAAAGCA GTTGAAAGCC TACAAGCCGT TAATCAAGGT CTTGAGCTGG GGATGCCAGT 960

TGATTTGCTT CAAGTTGACT TGACTCG C TTGGGAAATC CTCGGAGAAA TCACTGGGGA 1020

TGCTGCTCCA GATGAACTCA TCACCCAACT CTTTAGCCAA TTCTGTTTAG GAAAATAAGA 1080

AAAATCCATG ATCCTTCATT CGGTCATGGA TTTTATTGTC TTTATTAGTA ATCTGGTCTT 1140

AAGACCCCTG TTACAGTTGC CTTAGTTGCT TCGTAGTCGC CATCTACGAC AACCTTGATA 1200

ATGCGTTTGA CATCTTCTTC TGGTGCTGGA ACAAGAGGTA GACGAGTGGG TCCAGCTTCA 1260

AATCCCATAT AGTTAAGAAT TGCCTTAACT GGAGCAGGAC TTGGATAAGA GAAGAGAGCA 1320

TTAACCTTAG GAATGAATTT ACGCTGAATT GCTGCGGCTT TCTTCATATC GCTTTCTGCA 1380

ATGGCAGTAA ACATCTCGTG CATTTCATCC CCATTTGTAT GAGAGGCAAC AGAAATAACC 1440

CCATCCGCCC CAAGGTTCAT GGCATGGAAA GCATCTCCAT CCTCACCTGT ATAAATCAAG 1500

AACTCTTCAG GCTTGTGCTC AATCAAGTAA GCCATATTAG CCAAGCTAGT ACATTCTTTG 1560

ACACCGATAA TATTTGGATG GTCAGCCAAG CGAAGCATGG TTTCTGGAGT CAATTCGACA 1620

ACTACACGCC CTGGAATGTT ATAGATAATA ATTGGTAGGT CAGAAGCATC TGCAATAGCC 1680

TTAAAGTGCT GATACATCCC TTCTTGAGAA GGTTTGTTGT AGTAAGGAAC AATAGCAAGC 1740

CCAGCTGCGA AACCACCAAA TTCCGCTACT TCTTTGACAA ACTCAATAGA GTCACG 1796

(2) INFORMATION FOR SEQ ID NO: 54:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2337 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

167

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54:

CTTCGTACAG GTGGTTCCTA TGCAAGGGTG GAAGCCAATC GTCAGAACAA CAAGCATCTT 60

CATCAAGCCA GAACTGGAGC AATTACAAAA AGAAATTGCT GAAGAAGAAG CAAGCTTGGG 120

TTCAGAAGAA GTGGCTTTGA AGACCTTGCA AGATGAGATG GCCAGATTGA CCGAGTCATT 180

AGAAGCTATT AAATCTCAAG GAGAGCAGGC ACGTATTCAG GAGCAAGGCT TGTCCCTCGC 240

TTATCAGCAA ACTAGTCAGC AAGTTGAAGA ACTGGAAACT CTTTGGAAAC TCCAAGAAGA 300

GGAAATAGAT CGTCTTTCCG AGGGAGATTG GCAAGCGGAT AAGGAAAAAT GCCAAGAGCG 360

TCTTGCTGCA ATCGCCAGTG ACAAGCAAAA TCTGGAAGCT GAGATTGAAG AGATTAAGTC 420

TAATAAAAAT GCCATCCAAG AACGCTATCA AAACTTGCAG GAAGAGCTAG CGCAAGCTCG 480

TTTGCTTAAG ACAGAACTGC AAGGGCAAAA ACGTTATGAA ATTGCTGATA TTGAACGCTT 540

AGGCAAGGAA TTGGACAATC TTGATTTTGA ACAAGAGGAA ATCCAGCGCC TTCTTCAAGA 600

AAAGGTTGAC AATCTTGAGA AGGTTGATAC AGAATTGCTC AGTCAACAGG CGGAAGAATC 660

CAAAACTCAG AAAACGAACC TCCAACAAGG TTTGATTCGC AAACAGTTTG AGTTGGATGA 720

TATAGAAGGT CAGCTGGATG ATATTGCTAG TCATTTGGAT CAGGCTCGCC AGCAGAATGA 780

GGAGTGGATT CGCAAGCAAA CACGTGCTGA AGCTAAGAAA GAAAAGGTCA GCGAGCGCTT 840

TGCCGCCATC TACAAAGTCA ATTAACAGAC CAGTACCAGA TTAGCCATAC TGAAGCTCTA 900

GAAAAAGCGC ATGAATTGGA AAACCTCAAT CTGGCAGAGC AAGAAGTTAA GGATTTAGAG 960

AAGGCTATTC GCTCACTGGG TCCTGTCAAT ATAGAAGCTA TTGACCGGTA CGAAGAAGTT 1020

CACAACCGTC TGGACTTTCT AAATAGTCAG CGAGATGATA TTTTGTCAGC GAAAAATCTG 1080

CTCCTTGAAA CCATTACAAA GATGAATGAT GAGGTTAAGG AACGCTTTAA ATCAACCTTT 1140

GAAGCTATTC GTGAGTCCTT TAAAGTGACC TTCAAGCAGA TGTTTGGCGG AGGTCAGGCA 1200

GACTTGATAT TGACTGAGGG CGACCTTTTA CAGCTGGTGT GGAGATTTCT GTTCAACCTC 1260

CAGGTAAGAA AATCCAGTCG CTTAACCTCA TGAGTGGTGG TGAAAAAGCC CTATCGGCTC 1320

TTGCCTTGCT TTTCTCCATT ATTCGTGTCA AGACCATTCC TTTTGTCATC TTGGATGAGG 1380

TGGAAGCTGC GTTGGATGAA GCCAATGTTA AACGTTTTGG GGATTACCTC AACCGCTTTG 1440

ACAAGGACAG CCAGTTTATC GTCGTAACCC ACCGTAAGGG AACCATGGCA GCGGCCGATT 1500

CCATCTATGG AGTGACCATG CAAGAATCGG GTGTTTCAAA GATTGTTTCA GTTAAGTTAA 1560

AAGATTTAGA AAGTATTGAA GGATGACAAT TAAACTAGTA GCAACGGATA TGGACGGAAC 1620

CTTCCTAGAT GAGAATGGGC GCTTTGATAT GGACCGCCTC AAGTCTCTCT TGGTTTCCTA 1680

CAAGGAAAAA GGGATTTACT TTGCGGTGGC TTCGGGTCGG GGATTTCTGT CTCTGGAAAT 1740

CGAATTATTT GCTGGTGTTC GTGATGACAT TATTTTCATC GCGGAAAATG GCAGTTTGGT 1800

AGAGTATCAA GGTCAGGACT TGTATGAAGC GACTATGTCT CGTGACTTTT ATCTGGCAAC 1860

TTTTGAAAAG CTGAAAACGT CACCTTATAT AGATATCAAT AAACTGCTCT TGACGGGTAA 1920

GAAGGGTTCA TATGTTCTAG ATACGGTTGA TGAGACCTAT TTGAAAGTGA GTCAGCATTA 1980

TAATGAAAAT ATCCAAAAAG TAGCGAGTTT GGAAGATATC ACAGATGACA TTTTCAAATT 2040

TACAACCAAC TTCACAGAAG AAACGCTAGA AGCTGGTGAA GCTTGGGTCA ATGATAATGT 2100

CCCTGGTGTC AAGGCTATGA CAACTGGCTT TGAATCTATT GATATTGTTC TGGACTATGT 2160

CGATAAGGGT GTAGCTATTG TTGAATTAGC TAAAAAACTT GGCATCACAA TGGATCAGGT 2220

CATGGCTTTT GGAGACAATC TTAATGACTT ACATATGATG CAGGTTGTGG GACATCCTGT 2280

AGCTCCTGAA AATGCACGAC CAGAGATTTT AGAATTAGCA TAAGACTGTG ATTGGTC 2337

(2) INFORMATION FOR SEQ ID NO: 55:

168

SUBSTTTUTE SHEET (RULE 26) ;i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2162 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55:

CTAAAAGTGA AGCCCGATAG CGTCTCTCTC CTGCAAGGAT TTCATAACCA ATAACAGGAG 60

ATTGACGAAC AATAATCGGT TGAATGACCC CATTTTCTTT GATAGACTGT GCTAGTTCAT 120

CTAGCTTTTC TCTATCAAAT TCTTTTCGGG GTTGATAGGG ATTTTTTTGT ATATCTGTGA 180

TAGAAATCAT TTCAAATTTT TCCATGATTC TACACTAACA CATCTTTTCT CTTATGTAAA 240

GCTTTCTTTA CATAGATGTC AATTAAGATT CTAAATCACC TGAACTCTTG TTAAGTTTGA 300

TAGAGGTAGT TTCTTCTTTC CCGTTACGAT AGTAGGTTAT CTTAATGGTG TCTCCGATAG 360

AATGGTTGTA AAGAGCACTT TGTAAGTCTG TTGATGAAGC AATCTCTTTG TCATCTACTT 420

TTGTAATTAC ATCGTATTTT TCAAGGTGAC CATTGGCAGG CATATTACTT TGTACCGAAC 480

GAACAATTAC ACCAGATGTA ACATTACTTG GAATATTGAG TCTTCTGATG TCGCTTGTAC 540

TCACATTAGA TAAATTAACC ATCTGGATTC CCAAAGCTGG ACGCGTCACT TTTCCGTTTT 600

TTTCTAACTG TTCAATAATA TTGATAGCAT CATTTGCAGG AATTGCGAAA CCAAGACCTT 660

CTACAGATGT TCCTCCATTT GTAGCAATTT TACTTGAGGT AATTCCGATA ACCTGCCCTT 720

GAATATTGAT CAGTGGGCCG CCAGAGTTAC CTGGGTTAAT AGCAGTATCA GTTTGGATGG 780

CTTTTGTAGA AATAGCTTGT CCATCTTCCG ATTTTAAGGA TACATTTCTA TTGAGACTGG 840

ATACGATACC TTGAGTGACA GTATTTGCAT ATTCAGAACC TAACGGGCTA CCGATGGCAA 900

TAGCAGTTTC TCCTACAGTT AACTTACTAG AATCACCAAA CTCAGCTACT GTTGTCACTT 960

TTTCTGAAGA GATTTCGACG ACAGCAATAT CAGAGAAAGT GTCAGCTCCG ACAATTTCTC 1020

CAGGTACTTT AGTCCCATCT GACAATCGAA TATCTACTTT GCTGGCGCCA TTTATAACGT 1080

GATTGTTGGT GACGATGTAA GCTTCTTTAT CATTCTTTTT ATAAATAACT CCAGATCCTT 1140

CACTAGAGAT TCGCTGAGAA TCTGTGTCAG TATCATCATT GCCAAATACG CTATTTTGTC 1200

TGTTTGCCGA ATAAGTAATA ACAGAAACAA CAGCATCTTT TACTTTGTTA ACGGCCTGTG 1260

TTGTTGAATT TTCCGTTCCT TATAGGCAGT TTGTGTAATA GTACTATTGT TGTTAGAGTT 1320

GTTTACACTA CTTTTTTGAG TTAGTTGAGT TATTGAAAAA CTACCCAAGG CTCCACTAAA 1380

AAAGCTAATG ACGATAACGA CTAATAATTG AAACCATTTT TTGTAAAATG TTTTTAGATG 1440

TTTCATATTT GCCTCCATAT GTTTGAATTA CTGAAAGTAT AAACTGACTA GCTTAATTAT 1500

AACTTAAACA CAAAAGTTTT ACACAAACTG TGGATAACTC TTTTGAAACT GTGATTTTCT 1560

TAATTGAAAT CTATTTTTTA TTTTGTGAAT AAGATGTGAA AAAATAGAGA ATATGTTAGA 1620

ATAGAGTCAT GAAAATTAAA GTTGTAACAG TTGGGAAACT GAAAGAAAAG TATTTAAAAG 1680

ATGGTATCGC AGAGTATTCA AAACGAATTT CTAGATTTGC TAAGTTTGAA ATGATTGAGT 1740

TATCAGATGA AAAAACACCA GATAAGGCCA GTGAATCAGA AAATCAAAAG ATTTTAGAAA 1800

TAGAAGGTCA GAGAATTTTA TCAAAAATTG CTGACCGTGA TTTCGTTATT GTGTTAGCCA 1860

TTGAAGGGAA AACTTTCTTC TCAGAAGAAT TTAGTAAGCA GTGAGAAGAA ACTTCTATAA 1920

GGAAGGATGT CTACTCTTAC TTTTATTATT GGGGGAAGTT TAGGATTGTC ATCATCTGTA 1980 AAAAATAGAG CCAATCTTTC TGTCAGTTTT GGTCGCCTAA CCTTGCCTCA TCAGTTAATG 2040

AGACTAGTTC TTGTTGAACA AATCTATCGC GCTTTTACGA TTCAGCAGGG ATTCCCCTAC 2100

CATAAATAGA GAATTGACTT TTAATTGAAT TTTTGGTAGA ATAATTGTGT TAGGTCTCAT 2160

AG 2162

(2) INFORMATION FOR SEQ ID NO: 56:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1766 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56:

ATCGAATTTT CCAAAATGGG GAGCTAGAGC AGTGGAGTGA TTATGTGGCA GACGATTTGA 60

TTCAGC TAA TCATGAGATT GGACAAGGAA GTGCTGCTTA TAAAAACTAT GTGGCTGAAT 120

ATATTGTCAC TTTTGACTTC GTTTTCCAAC TCTTAGGACA AGGAAACTAT GTGGTTAGCT 180

ATGGTCAGAC TCAGATTGAT GGCGTTGCTT ATGCCAAGTA CGATATCTTC CGTTTAAAGA 240

ACGGGAAAAT TGTGGAGCAT TGGGATAATA AGGAAGTCAT GCCTAAGGTA GAAGACTTGA 300

CCAATCGAGG GAAGTTTTAA ATTGAGGACA AAGAATGATT GAATACAAAA ATGTAGCACT 360

GCGCTACACA GAAAAGGATG TCTTGAGAGA TGTCAACTTA CAGATTGAGG ATGGGGAATT 420

TATGGTTTTA GTAGGGCCTT CTGGGTCAGG TAAGACGACC ATGCTCAAGA TGATTAACCG 480

TCTTTTGGAA CCAACTGATG GAAATATTTA TATGGATGGG AAGCGCATCA AAGACTATGA 540

TGAGCGTGAA CTTCGTCTTT CTACTGGTTA TGTTTTACAG GCTATTGCTC TTTTTCCAAA 600

TCTAACAGTT GCGGAAAATA TTGCTCTCAT TCCTGAAATG AAGGGGTGGA GCAAGGAAGA 660

AATTACGAAG AAAACAGAAG AGCTTTTGGC TAAGGTTGGT TTACCAGTAG CCGAGTATGG 720

GCATCGCTTA CCTAGTGAAT TATCTGGTGG AGAACAGCAA CGGGTCGGTA TTGTCCGAGC 780

TATGATTGGT CAGCCCAAGA TTTTCCTCAT GGATGAACCC TTTTCGGCCT TGGATGCTAT 840

TTCGAGAAAA CAGTTGCAGG TTCTGACAAA AGAATTGCAT AAAGAGTTTG GGATGACAAC 900

GATTTTTGTA ACCCATGATA CGGATGAAGC CTTGAAGTTG GCGGACCGTA TTGCTGTCTT 960

GCAGGATGGA GAAATTCGCC AGGTAGCGAA TCCCGAGACA ATTTTAAAAG TGCCTGCAAC 1020

AGACTTTGTA GCAGACTTGT TTGGAGGTAG TGTTCATGAC TAATTTAATT GCAACTTTTC 1080

AGGATCGTTT TAGTGATTGG TTGACAGCTA CAATGACATT GGTCGGTTCC TTGAGCAAGA 1140

GATAGATTAG CCAGACAGTC ATGCCCAAAA TCCCTCCAGG TAAGAGCATA GACCGTTGCA 1200

CATTAAGTAC GATTAAAAAA GTGATAATGG CAAGAAAACT TGCTACTGCT TGTAATAAAA 1260

AGGTTGTTAG TGTCATATTA GTTCATCAAT ACCAAGGCGA CAGAAGTTCC TGCCCCTAAA 1320

GCGAGGGTAA TGAGCAGGGA TTCAAACATC TTACTCATAC CAGAGTTTAT GTGGTTGGTC 1380

ATAATATCAC GGACCGCATT GGTCAAGGCA ATACCTGGTA CAAACGGCAT GACCGCACCA 1440

GCTATAATCA AATCTGCCGT TGAAGGAAAA CCTGTGTAGC GAGCCCAAAA CTGGGCAATT 1500

ATCCCAAAGA CAAAAGCTCC AGCAAAGGCT GTCACAAAGG GAATTCGGAT AAATTTTTCC 1560

ACA AGAGGG AAAAGGCAAA ACCAAATAAG GTCGCCACTC CTGCCCCAAG TGCGTCGTAG 1620

170

UBSTTTUTE SHEET (RULE 26) ATATTTCCGC TAAACATAAC TGAAAAGAAA GGAGCACTAA AGGTCGCAGC CAGAGTTACC 1680

TGCAACTTAG TATAGGGAAG GGGTTGAGCT TGCAAGGCCG TCAATTGCTT AAAGGCTGTT 1740

TCTAAGTCAA TCTGCCCCCC AACTGG 1766

(2) INFORMATION FOR SEQ ID NO: 57:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1705 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:57:

CTCTGACGGA GGCTGGTTAT GTGGGTGAGG ATGTGGAAAA TATACTCCTC AAACTCTTGC 60

AGGTTGCTGA CTTTAACATC GAACGTGCAG AGCGTGGCAT TATCTATGTG GATGAAATTG 120

ACAAGATTGC CAAGAAGAGT GAGAATGTGT CTATCACACG TGATGTTTCT GGTGAAGGGG 180

TGCAACAAGC CCTTCTCAAG ATTATTGAGG GAACTGTTGC TAGCGTACCG CCTCAAGGTG 240

GACGCAAACA TCCACAACAA GAGATGATTC AAGTGGATAC AAAAAATATC CTCTTCATCG 300

TGGGTGGTGC TTTTGATGGT ATTGAAGAAA TTGTCAAACA ACGTCTGGGT GAAAAAGTCA 360

TCGGATTTGG TCAAAACAAT AAGGCGATTG ACGAAAACAG CTCATACATG CAAGAAATCA 420

TCGCTGAAGA CATTCAAAAA TTTGGTATTA TCCCTGAGTT GATTGGACGC TTGCCTGTTT 480

TTGCGGCTCT TGAGCAATTG ACCGTTGATG ACTTGGTTCG CATCTTGAAA GAGCCAAGAA 540

ATGCCTTGGT GAAACAATAC CAAACCTTGC TTTCTTATGA TGATGTTGAG TTGGAATTTG 600

ACGACGAAGC CCTTCAAGAG ATTGCTAATA AAGCAATCGA ACGGAAGACA GGGGCGCGTG 660

GACTTCGCTC CATCATCGAA GAAACCATGC TAGATGTTAT GTTTGAGGTG CCGAGTCAGG 720

AAAATGTGAA ATTGGTTCGC ATCACTAAAG AAACTGTCGA TGGAACGGAT AAACCGATCC 780

TAGAAACAGC CTAGAGGTGA CTATGGAACT TAATACACAC AATGCTGAAA TCTTGCTCAG 840

TGCAGCTAAT AAGTCCCACT ATCCGCAGGA TGAACTGCCA GAGATTGCCC TAGCAGGGCG 900

TTCAAATGTT GGTAAATCCA GCTTTATCAA CACTATGTTG AACCGTAAGA ATCTCGCTCG 960

TACATCAGGA AAACCTGGTA AAACCCAGCT CCTGAACTTT TTTAACATTG ATGACAAGAT 1020

GCGCTTTGTG GATGTGCCTG GTTATGGCTA TGCTCGTGTT TCTAAAAAGG AACGTGAAAA 1080

GTGGGGGTGC ATGATTGAGG AGTAATTTAA CGACTCGGGA AAATCTCCGT GCGGTTGTCA 1140

GTCTAGTTGA CCTTCGTCAT GACCCGTCAG CAGATGATGT GCAGATGTAC GAATTTCTCA 1200

AGTATTATGA GATTCCAGTC ATCATTGTGG CGACCAAGGC GGACAAGATT CCTCGTGGTA 1260

AATGGAACAA GCATGAATCA GCAATCAAAA AGAAATTAAA CTTTGACCCA AGTGACGATT 1320

TCATCCTCTT TTCATCTGTC AGCAAGGCAG GGATGGATGA GGCTTGGGAT GCAATCTTAG 1380

AAAAATTGTG AGGAAAAGAA AATGGCAAAA ACAATTCATA CAGATAAGGC CCCAAAGGCT 1440

ATCGGGCCCT ATGTTCAAGG AAAAATCGTT GGCAACCTTT TGTTTGCTAG CGGTCAAGTT 1500

CCCCTATCCC CTGAAACTGG GGAAATTGTA GGAGAGAATA TCCAAGAACA GACAGAGCAA 1560

GTCTTGAAAA ACATCGGTGC TATTTTGGCA GAAGCAGGAA CAGACTTTGA CCATGTTGTC 1620

AAAACAACTT GTTTCTTGAG CGATATGAAC GACTTTGTTC CTTTTAATGA GGTTTACCAA 1680 ACGGCCTTCA AAGAGGAATT CCCAG 1705

(2) INFORMATION FOR SEQ ID NO: 58:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1673 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 58:

ACGTTTTGGG AACTGTTCGG ATAGCAGATT CCGAACAAAC TGATAATGGT TGGCAAAATC 60

ATTATTCCTA ATAGTAACGA AGCTGGTTAG GACAACTCAT GCCATTTCCT AAAAAGGTTT 120

TAATCCAAGG CACCAATAAT TGTAGGCCGA AAAAACCATA AACAATAGAT GGAATGGCTG 180

CCATCAAGTT GATAGCTGAT TTTAAGAAGC TATAGACGGG CTTTGGACAA TTATAAACCA 240

TAAACACCGA TGTCAAGATC GCCTGTTGGC ACCCCAATCA CAATCGCTCC TAAGGTCGAA 300

TAAATAAGGA ACCAACGATC ATTGGTAAAA TACCATAGCT TGCCGGAATG TTCGTTGGCG 360

ACCAATCACT GCCTAATAAA AAACGGGCAA AGCCGTAGTT AGCTATGAAA GGTAAGCCAT 420

TACTAAAAAT AAAGAAACAG ATTAGCAAAA TAGCTACAAC AGCTACTGTT GCACTCATGA 480

AAAAAATTGC CCTAAAAACT GCTTCTTTGA AGGCTTGTTT TGTCACATCT TGTCCTTTCT 540

AGTGAAGAAA GTAAGGGAGA TACGACACCT CCCTACTTGC CTTCTTTATC TTATTGTACG 600

ATGAAACGTC TGCATCTCTT TAGAGATTTA TGGAGCAAAC ATTTTATTTA ATCTTGTCCC 660

AGGTGGTTAA TTTGCCACTA AAAACGTCCG CAAGTTCAGC CATACTGACT TGGCTTGCCT 720

TATTGTCATT ATTGACCACA ACAGCAATAC CGTCTAAAGC AATAGCATCA TGGGTGAGAC 780 TCTTACCTTC TTCAGGAGTT AATTCCCTAG AAACCATACC AATATCAGCG GTTTTCTCCT 840

TAACAGCGGT AATACCTGCT GAAGACCCAT TAGAGGTAAT ATCAATCGTA ACTTCTGGAT 900

TTTCTTTTTT ATAAGCTTCT GCTAATTTTT CCATTAAAGA AGATACTGAA GTGGAACCTA 960

CAACAGACAA CTTGCCTGAT AAGTGTTGGC TTGTA ATTC TGTGGTTTCG GTTTTAGCTT 1020

CAATAAATTT ATTATCTGTG ACCACTTGTT GACCTTGTTT GGAGTGGATA AAGCTGATAA 1080

AATCTTGACC TAGCTTGGAA AGATTAGAAG ACCAAACAAT GTTGAAGGGA CGTTGAAGAG 1140

GGTATTCACC ATCTAAAACT GTGTCTCGAC TAGCCTTGAC ACCATCAATC TCTAAAGCCT 1200

TGACAGATTT CGTTAAAGAT CCCAAGGAGA TGTAGCCGAT AGCATTAGCA TTCCCTTGAA 1260

CTGCTGAGAG AACACCTTCT GTACTATTTT GAATCACAGC TGTTTTGGCA GTGTAGTCAA 1320

TTTTTTTATC ACCGTCTTTT TTGAGAATCC CTGTGATTTC TGTGAAGGCA CCCCGTGTTC 1380

CAGAGCCATT TTCTCGTGAA ATCACCTCAA TCGTTCCTGG AGCTGACTGT TTGGAAGCAG 1440

CTGACTGATT GCCACAGGCA ACAAGCCCAA ATCCTGATAA GCCAATGGCT GCAAGAGTAA 1500

GCATTTTTTT GAATTTCATA ATAATCACCT TTATCTCTAT GTATTTTTCT TGTGTAGGCT 1560

TACTACATTT ATAGTCTAAC AAGTCTTTGT AAAGGTTTAT CCCTGATTCA TGTAAAGATT 1620

GTGTAAAGAA TCAAAAAAAG CCACTTTTGA AAAATGGCTG CCCCTAAAAA TAG 1673

(2) INFORMATION FOR SEQ ID NO: 59:

172

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1702 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 59:

CTTTTTTATT TCACAACAAG TTCATAACGT GTCTTACTGG TGAAGGTTTG ACCAGCTTTA 60

AGAATGACTT GGCCTTTAAG GTCACTGTGA ATGGCATCTG GTAAAGCTTG CGCTTCAAGA 120

GCAATCCCAT TGTGCTGTAG CATTGGCTGA CCTCCTATGA TGACACTTTC ATCCACAAAG 180

TTTGCTGTGT AGACCACAAA GCAAGGAGCT TCTGTCTTGA AAAGCAGGAA GCGACCTGAA 240

TTTTGGTCAT AAAGGAATCC AGCATTGTCA TGGCCTGCAG GAAGGGCAAA TGGATGATCC 300

AAACCTGATG CCAGCTGGAT TTGCTCATCT TCTTCTGCAA AGATATCCTT CAACAAGGCA 360

CCATTGTAGA TGTGTTTGAC CACATCACGG TTGGCTTCTG GAGTTTTGGC AGGAACACCG 420

TCAGGAGCGA TTGAGTAAAT GCCCTCTGTG TTTAGTTGGA AGACATGACG GTCAATCGTC 480

TGCGTGAAAT CACCAGACAA GTTGAAATAG CTGTGGTTGG TTGGATTGAC CAGCGTATCC 540

TGATCGGTCG TTACCTTGTA GATCGAATTC ATGGAGGCAC CAGTTTCTTC CAAGTGATAA 600

CTGATCGCCA AATCTTGAGA TTTCCAGGGA ACCCTCCTGT CCCATCTGTA CGCTCTGTGT 660

AGAGAGTCAA GCCATGATCG CTTACTTCTT CAACTTCAAA CAAGCTGGAA TCCCAACCAG 720

TTGAACCACT GTGATTACAG TTGCTAGCAT TATTAACCTC AAGGTCATAG GTCTTACCAT 780

TGAGCTCAAA GGTCGCACCT GCAATACGAC CCGCTACAGG ACCTACACTT GCTCCATGCT 840

TGGGACTATT GCCTACATAA CTATCAAAGT CATCAAATCC CAAGATAACA TTGGCAAAAT 900

TTCCAGCCTT GTCAGGTGCG ACATAGCGCA AGATAGTCGC ACCATAAGTC ATAACCTCAA 960

GTTGGTAGCC ACCGTCTGTC TCAAATCGAT AGGCCAAGAC ATCCTCACCC TCAACATTTC 1020

CAAATACACG CTCTGTGTAT GCTTTCATTC TGTTCTCCTT TTACTATTTC TCTCAAGCAA 1080

AC AACCATA GAAAGCGTAC TGACAATCTA TGGTTTATCT GATAATTTAC AAATCCTCTT 1140

GTCAAGAATT CATAAACACT GTCTTACTTT TGATATTCGT GAATTATGAC ACCTTGTACT 1200

ACACGGTTTA CTGTACCTGT AGGAGACGGT GTATCTGGTT TATTTTCTAC CTTGAGTGAA 1260

GTCAATAGGG CAAAGAGTTG GGCATAAACG ATGTAAGGGA AGACACGGTA AATATCATTC 1320

AAGACACCGC CACAACCAAG GGCCACTTCT TTGACATTTT CAAGACCAAA AGCTTGATCA 1380

CTCAAAAGCA CAACACGACG AGCAATCTGG TCACCAGCAA CTTCACGAAC CAAGTCCAAG 1440

TCGTACT AC GAGTGTAGTC CGTCGTTGTA CCAAAGACCA AAACAACTGT ATTGTCGTTG 1500

ATAAGAGATT TTGGACCGTG ACGGAAGCCA ACTGGGCTTT CATACATGGT CGCAACTTGA 1560

CCAGCAGTTA ATTCCAAAAT CTTGAGCTGA GCTTCATGAG CAAGTCCAAA GAAAGGACCA 1620

GCGCCTAGAA TAGATGACAC GGTTAAAGTC TAAATCAACG AGATCTTTGA CATCTTCTGC 1680

CTTGTCTAAA ACTTTACGGG CA 1702

(2) INFORMATION FOR SEQ ID NO: 60:

(i) SEQUENCE CHARACTERISTICS:

173

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 1940 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 60:

TGCAGGATTT GATTTGGACG ACTTTTATTA TTACCAGATT CGCCTAGGAA TAGAAAAAAG 60

AGCCCAAGAG TTGGACTATG ATATCTTGCG CTATTTTAAT GACCACCCTT TTACCCTAAG 120

CGAGGAAGTG ATTGGGATTC TCTGCATCGG AAAGTTTAGT CGAGCTCAGA TTTCTGCCTT 180

TGAAGAATAC CAAAAGCCTC TTGTATTTCT AGACAGCGAT ACACTTTCCC TGGGACATAC 240

CTGTATTATC ACGGATTTTT ACACTGCTAT GAAACAGGTT GTCGATTATT TCCTCAGTCA 300

AGGAATGGAC CGTATCGGGA TTCTAACAGG CCTTGAAGAA ACAACAGACC AAGAAGAAAT 360

CATTCAGGAC AAGCGTCTAG AAAACTTCAA AAACTACAGT CAAGCGAGGG GAATCTATCA 420

TGATGAACTG GTCTTTCAAG GAAGATTTAC TGCCCAGTCT GGCTATGACT TAATGAAGGA 480

GGCCATTCAG AGCTTGGGAG ACCAACTTCC GCCAGCATTT TTCGCAGCCA GCGATAGTTT 540

AGCTATCGGT GCCCTCCGTG CCCTCCAAGA AGCTGGAATC AGCCTGCCAG ATCGCGTCAG 600

CCTCATTTCC TTTAACGACA CTAGTCTGAC CAAACAGGTC TATCCTCCCC TCTCTAGTAT 660

TACAGTTTAT ACTGAAGAAA TGGGCCGAGC AGGTATGGAT ATTCTTAACA AGGAAGTCCT 720

CCACGGTCGG AAAATCCCTA GCCTGACCAT GCTGGGAACC AGACTGACAT TAAGAGAAAG 780

TACCCTAAAT CAAGAATAGG ATAACATAAA AAACGAATAG AGTTCTAAAA CTCCTATTCG 840

TTTTTTATTC GATTACAATC ATAGACTTAA TGGTCTTACG TTCATCCATA TCTTTGTAGG 900

CTTGGTCGAT ATCTTCCAGT TTATAACTTG AAGTAAAGAC GCGACCTGGA TTGATATCAC 960

CATCAAGGAC GGCTTTTAGT AAAAATTGCT TATCGTATGT TGTAGCAGAA GCTGCCCCAC 1020

CTGCTACAGA GA ATTTTGC A AAATGTCG AACCAAGAGC ACGATTATTA TAGTGTGGGA 1080

CTCCTACAAA GCCCATACGC CCTCCATTAT GAAGAACACC TAGCGCCTGT TCTATAGCAG 1140

CCTCCGTACC AACACATTCA AGTGCTGCGT CTGCTCCTCC GCCGAGGATT TCACGCACCT 1200

TGGTAATTCC TTCTTGACCA CGTTCTGCAA CAACAGCTGT CGCACCTGAC TCCATAGCCA 1260

TCTTTTGACG GTCTTCATGA CGGCTCATAA GGATAATTTG TGATGCTCCA CGCATCTTAG 1320

CCGCGATGAC AGCACATTGA CCAACAGCCC CATCACCGAT AACAACAACC TTGTCCCCTT 1380

TTTGAACATT TGCAACACGC GCCGCATGAT AGCCTGTCGG CATGACATCT GCAAGAGTCA 1440

AAAGGGACTT GAGCATCCCT TCTGTATAGT CAGAAGGTTG ACCAGGGATT TTAACCAGCG 1500

CCCAGTTTGC ATAGTGGAAG CGAATATATT CTGCCTGAAA ATCACCCCCC AAATTATTGC 1560

CAATATGATT GTCGCAAGAA CCGTCAAATC CAGCAAGACA GGCATCACAC TCACCACATC 1620

CATGTGTAAA AGGGACAATC ACAAAATCAC CTGGTTTCAC CGTCGTAATG GCTTCCCCAG 1680

CTTCTTCAAC AATCCCAATC GCTTCGTGTC CACTTATTTT TTGTGTCCAA CTTTCGTTTT 1740

CCNTGGATTA CGGTACCTCC ATAAATTTGA ACCACAAACG CACGCACGAA CCACACGAAT 1800

AATCACATCA TCCGCTTCTA TTATTTGCGG ACGTTCAATG CTAGCAAGTC CAACCTGACC 1860

TGCCTTTGTA TATACTGCTG ATTTCATTTA AAATTTTCCT TCCTTATAAA GTTTAATTTT 1920

GAGATTTAAA CGATTTAAAG 1940

174

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 61:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2051 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61:

ATCGAATTTT TCTAGCCAGG CTACAGTTTT GGCAAGTAAG GTTTCATCTC AGGCAGTCAA 60

CTGGGTGAGT GCCTTTATTA GCGGAGCTTC TCAAGTGATT GTTGCCTTGA TTATCGTTCC 120

TTTCATGCTC TTTTATCTCT TGCGTGATGG GAAAGGCTTG CGTAACTATT TGACCCAATT 180

CATTCCAAGA AAATTGAAGG AACCTGTTGG ACAAGTTCTA TCAGATGTGA ATCAACAGTT 240

GTCCAACTAT GTTCGAGGGC AAGTGACAGT GGCTATTATT GTAGCAGTAA TGTTTATCAT 300

CTTCTTCAAG ATTATTGGTC TACGCTATGC GGTTACGCTG GGGGTTACTG CTGGTATTTT 360

AAATCTGGTC CCTTATCTTG GTAGCTTTCT AGCCATGCTT CCTGCCCTAG TATTGGGTTT 420

GATTGCTGGT CCAGTCATGC TTTTGAAAGT AGTGATTGTC TTTATTGTAG AACAAACTAT 480

TGAAGGCCGT TTTGTCTCTC CATTGATTTT GGGAAGTCAA TTAAACATCC ACCCTATTAA 540

TGTTCTCTTT GTTTTGTTAA CTTCAGGATC TATGTTTGGT ATCTGGGGAG TTTTACTTGG 600

TATTCCGGTT TATGCCTCTG CTAAGGTTGT CATTTCAGCC ATTTTCGAAT GGTATAAGGT 660

AGTCAGTGGT CTATATGAAT TAGAGGGTGA GGAAGTCAAG AGTGAACAAT AGTCAACAGA 720

TGTTACAGGC TTTGGAGGAG CAAGATTTAA CTAAGGCTGA GCATTATTTC GCCAAAGCTT 780

TAGAAAATGA TTCAAGTGAT CTTCTGTATG AGTTGGCAAC TTATCTTGAA GGGATTGGTT 840

TCTATCCTCA GGCCAAGGAA ATTTACCTGA AAATTGTAGA AGAATTTCCA GAGGTTCATC 900

TTAATCTAGC TGCAATGGCT AGCGAGGATG GTCAAATAGA AAAAGCCTTT AACTATCTTG 960

AGGAAATCCA AGCTGACAGT GACTGGTATG TCTCGCTCTT TGGCTCTGAA GGCAGACCTA 1020

TACCAGCTGG AAGGTTTGAC AGATGTGGCA CGTGAGAAAT TATTGGAGGC CTTGACCTAC 1080

TCAAAGGATT CTCTCTTGAT ATTGGGTTTG GCAAAGTTGG ATAGTGAGTT GGAAAATTAC 1140

CAAGCGGCTA TTCAAGCCTA TGCCCAGTTA GATAATCGCT CGATTTATGA GCAAACGGGC 1200

ATTTCCACCT ATCAACGAAT TGGCTTTGCC TATGCTCAGT TAGGGAAATT TGAAACGGCT 1260

ACTGAGTTTT TAGAAAAAGC CCTGGAGTTA GAATACGATG ACTTAACAGC TTTTGAGTTG 1320

GCCAGTCTTT ATTTTGATCA AGAAGAATAT CAAAAAGCCA CCCTCTACTT TAAGCAGCTT 1380

GATACCATTT CTCCTGACTT TGAAGGCTAT GAGTATGGGT ACAGTCAGGC TTTACATAAG 1440

GAACATCAAG TTCAAGAAGC CCTGCGTATC GCTAAGCAAG GATTAGAGAA AAATCCCTTT 1500

GAAACTCGCC TCTTGCTAGC TGCTTCACAA TTTTCTTATG AATTGCATGA TGCTAGTGGT 1560

GCAGAAAATT ATCTCCTTAC TGCAAAAGAA GACGCTGAGG A ACAGAAGA AATCTTGCTT 1620

CGTTTAGCCA CTATTTATCT GGAGCAGGAG CGTTATGAGG ATATTCTAGA CTTGCAGAGT 1680

GAGGAGCCAG AAAATCTTTT GACCAAGTGG ATGATTGCTC GTTCTTATCA AGAAATGGAC 1740

GATTTGGATA CTGCTTATGA GCATTATCAA GAGTTGACAG GAGATTTGAA GGACAATCCA 1800

GAATTTCTGG AACACTATAT CTATCTCTTG CGTGAATTGG GACATTTTGA AGAAGCAAAA 1860

GTCCATGCTC ACACTTACTT AAAACTGGTT CCAGATGATG TGCAAATGCA AGAACTGTTT 1920

175

SUBSTTTUTE SHEET (RULE 26) GAGAGATTGT AAGAATGTTT AAACATATAG AACTGTAGTT TATCTCTTTT GATAGCTACG 1980 GTCTTTATTT GTACATGGTA GAATCTTTTT ACAAAAATAC TTGGTAATCT TGTTTATTCA 2040 TGCCATAATA G 2051

(2) INFORMATION FOR SEQ ID NO: 62:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1318 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62:

CTTTAGCAAT CAGTTTATTG GGAGATTTGA CTGCCACTTC TGTTGGAACC TTGATAATCT 60

TTTTACCCTC AAAGCGTTCC ATACCAGAAA TCTTAACATC AACTGCTAAA ATAACTACAT 120

CCGCTGCATC AATCTGCTCT TGACTCAATT CATTTTCTAC CCCTATTGTC CCCTGAGTCT 180

CAACATGAAT CACATGTCCA GCTACCTTTG CGGCATTCTC TAATTTTTCC TGTGCAATAT 240

AAGTGTGGGC AATTCCCATA GTACAAGCTG CAACACCAAC AATTTTCATA CGGATACCCT 300

CCAAAATTTT TTCTTATTAA CAAAAAGCTG CAATCACATC ATCAGATGTC TGAGCCCGAA 360

CTAATTTGGC AACAACTTCG TCATTACCAA GTTTTCGAGC AAAGAGTGAT AAGGTCTTCA 420

AATGCTCCCT AGCAGCTTCT GTATCATCAC CAACTGCAAA GAGTACAATT ACTTTGACCC 480

CTTTCCCATC AATGGTCTCC CAAGGAATCT CATTGTGATT TATAGCTATG ACTACCCCCG 540

CCTTCTCCAC AGCAGAACTC TAGCTATGGG GAATAGCAAT ATAATTCCCA ATACCGGTCT 600

GTCCTTCTGC CTCTCTCTGA TAAAGACCTT CGATAAATTG GTCTCTATCA GACACATAAC 660

CCGTCTCAAC CAATAGTATG AGCTAATGCC TCAAAAACCT CTTCTTTGCT CTGCATCTGT 720

AAATCCGTCT GGATCAGACT CACATTAAGA ATATCTTTGA TTTCCATATA TTATCTCCCG 780

TAATTCTTCT TTTGTTAACT GTTTTAATTG ATTTATGAAT GATTCATCTG CTAGTCTTCT 840

CATCAATGTT TTAATACATG ACTTGTCCTG TGATACTGCA ATGGCCAAAC CGATAATAAG 900

GTCAACACAC TGGATATCCT TCGACCATTC TCTGATAGGT GGTTTTAATC TAGTAATCAC 960

TAAGACATGA TGTTGAAAGT TTCCTTCACA ATGTGGTAGA AGAACACCTT TAGCAACCTC 1020

TATACTTCCC TGTCTCTCAC GGTAATA AG AAGCTCTTCT ATTTTTTCTG TATCTTCAGA 1080

AACAAGAAGG CTGATTTGAT TTGCTAATTC TTTGTAGGCT TCTTGACGAT TTTGAACAGA 1140

TATATCCATA AGGACAAGCG AAAGATTATT CATAGTTTAT CTCCTGAATT TTTGCTTGAA 1200

GACGTTGTTT ATCACCCTCG GTTAGAAAAG CACTAACTAG GACAAACGGG ACACTTGCTG 1260

GTTCCTGCAA AGCTACCGTC GTCACAATGA AATCTAAATC TGGATATAGA TTTATCAG 1318

(2) INFORMATION FOR SEQ ID NO: 63:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2077 base pairs

(B) TYPE: nucleic acid

176

SUBSTTTUTE SHEET (RULE 26) (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:63:

CTAGTCTTGG CTACTGTCTA AGTTGGCTTG TGCATAAGCC TGCCAGATTT TTTGTTGGGG 60

TTTGGCAAGT GGGTAATTCT TGAATTCTTC TGGTGAAAGC CAACGAACTT CCCTATCTGA 120

AAAATCATGG AAGTCACTCA CCTGACCTGC TACAATCTGT ACATGCCATT TTCGATGACT 180

AAAAACATGC TGGACTGTAT CAAAACAAAC ATCAAGCCAA TCAACATCTA GGTCATAGTC 240

CTGCTGGAAA CTCTCTTCTG GGACTGGGGC CAGAGTTCAC ACTTTCTTCC GCAACCTGAT 300

GAAAGAGGTC AAACTGCTCT TCTTGCGAAA AGTTATCAAC TTCTATAAAG GGGAAATGCC 360

AAAAACCTGC CAAGAGCTTT TCGCTTTCAT TTTTTTCAAG TAAAAATTGT CCTTGAGAAT 420

TTTTCACAAC TAAGGCTTTA AGATAAATAG GAACCGGCTT TTTCTTAGGA GATTTAATTG 480

GATAACGGTC CATGGTTCCA TTCTGATATG CCGCACTAAA GTCCTTGACT GGGCTTTCTT 540

CAGGTCTGGG ATTTACAGGA GACTCAATAT CAGACCCTAA GTCCATCAAG GCTTGATTAA 600

AATCACCCGG ACGATCTGGA TTAATCAAGA TCTCCATCAT TGCCTGAAAA ATTTTTCGAT 660

TACTTGGAAT CCCAATATCG TGGTTGACTT CAAACAGACG CGCCAAGACC CGCATGACAT 720

TACCATCTAC AGCTGGCTCA GGCAAGTTAA AAGCAATACT GGAAATGGCT CCTGCTGTGT 780

AAGGTCCAAT CCCTTTCAAG CTGGAAATTC CTTCATAGGT ATTTGGAAAT TGGCCACCAA 840

AGTCAGTCAT AATCTGCTGG GCTGCAGCCT GCATATTGCG AACTCGAGAA TAATAACCCA 900

AGCCCTCCCA AGCTTTCAGT AAACTCTCCT CAGGCGCAGT TGCCAGACTT TCGACAGTTG 960

GAAACCAGTC CAAAAATCTT TCGTAGTAAG GGATAACTGT ATCCACCCTG GTCTGCTGAA 1020

GCATGATTTC AGATACCCAG ATGTGATAAG GATTTTTACT TCTCCTCCAA GGCAAATCTC 1080

TTTTGTTTTC ATCATACCAA GCGAGAAGTT TTCTCACCGG AAAGAAATGA CTTTCTCCTC 1140

CGGCCACATG ACGATACCGT ATTCTTTCAA ATCCTAACAT ATCTCTAGTT ATAACACAGA 1200

AGGTTTCACC TGTCTTTGTA TCTGATTTAT AATATTTTCA ATAGATAGTA TATAACTTTT 1260

CCTATCTACT TATACTCCAA TGAAAATCCA AAGAGCAAAC TAAGAAGCTA GCCGCAGGTT 1320

GCTCAAAACA CTGTTTTGAG GTTGTGGATA GAACTGACAG AGTCAGTATC ATATTACCTA 1380

CGGCAAGGTG AAGCTGACGT AGTTTGAAAA GATTTTCGAA GAGTATAAAT CTTATTGATG 1440

AACTGCTTGC AGTCTGAGAA AAAATGAGCT TGGATATTAT TTCCAAACTC ACTTAAAGTC 1500

AATTTCAATC CACTAGAACA AGCCTAGTAC AGTTCCATCG CTTTCAACAT CCATGTTGAG 1560

AGCTGCTGGA CGTTTTGGAA GACCTGGCAT GGTCATAACA TCACCAGTTA AGGCAACGAT 1620

GAAGCCTGCA CCTAATTTTG GTACCAATTC ACGAATGGTA ATTTCAAAGT TTTCTGGTGC 1680

TCCAAGCGCA TTTGGATTGT CTGAGAAACT GTATTGAGTT TTAGCCATAC AAATTGGCAA 1740

TTTGTCCCAA CCGTTTTGAA CGATTTGAGC AATTTGTGTT TGAGCTTTCT TCTCAAAGTT 1800

CACTTTGCTA CCACGATAGA TTTCAGTGAC AATTTTTTCA ATCTTTTCTT GGACAGAAAG 1860

GTCATTATCG TACAAACGTT TATAGTTAGC TGGATTTTCA GCAATTGTCT TAACAACTGT 1920

TTCGGCAAGT GCTACTCCAC CTTCTGCTCC ATCAGCCCAG ACACTAGCCA ATTCAACTGG 1980

TACATCGATT GAGGCACAGA GTTCTTTTAA GGCTGCAATT TCAGCTTCTG TATCAGATAC 2040

AAATTCGTTA ATAGATACAA GCTAATGGAA TACCGAA 2077

(2) INFORMATION FOR SEQ ID NO: 64:

177

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1887 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64:

CTCAAAACNC TGCTTTGAAG AGATTTTCAA AGAGTACAAG AAGTTTAGTT ATTAGCGTTC 60

TTACCGCTTG TAAACTAGAT TTCTCATAAA ATAGAATCTT TTCCTTTTAG TTGTAAACTA 120

GTCTGGGAGA GTAGAGAGGT TTGAGATACC TTTCTAGCTT TTGGATTATC ATCTAAGAAG 180

AGTAATTTCC CTTGCATTAA AAAGGGGAAA AAGAGACACG AAATGACTAT AATGGGTGAC 240

AATGGGGGAA GGGATAGACA AGAGATTTTA TCCACATATG AAAAAAGGAG GTTAGGAAAG 300

AGTTATATAT CCTATATTAT ATAAATAATC AATTGCGCAG AAATTTGGTA AGAATTCATG 360

CGTCAACTCA TAAAGAACTA CTTAAAAAAT TCACAGTATT CATAATTATT TTCGAGGAGA 420

AAAACAGTGA AAAAAAGAAA AAAGCTTGCT CTGTCTCTTA TCGCTTTTTG GCTGACGGCT 480

TGTTTAGTAG GCTGTGCTAG CTGGATTGAT CGTGGAGAAT CCATAACGGC TGTTGGCTCA 540

ACTGCCTTGC AACCCTTGGT TGAAGTAGCG GCAGATGAAT TTGGCACCAT CCATGTTGGA 600

AAAACGGTCA ATGTCCAAGG GGGAAGTTCT GGTACAGGCT TGTCCCAGGT TCAGTCTGGG 660

GCAGTTGATA TAGGAAACTC AGATGTATTT GCTGAGGAAA AAGACGGAAT TGATGCTTCT 720

GCTCTTGTTG ACCACAAGGT CGCGGTAGCT GGCTTGGCTC TGATTGTCAA TAAGGAGGTT 780

GATGTTGATA ACCTAACGAC AGAGCAACTT CGTCAAATCT TCATAGGTGA GGTAACCAAT 840

TGGAAAGAGG TTGGTGGTAA GGACTTACCC ATCTCTGTTA TCAATCGGGC AGCCGGCTCT 900

GGCTCTCGTG CTACCTTTGA TACTGTCATT ATGGAAGGTC AGTCTGCCAT GCAAAGTCAG 960

GAGCAGGATT CAAATGGAGC GGTAAAATCA ATCGTATCAA AAAGTCCAGG AGCTATCTCT 1020

TATTTATCTC TTACCTATAT AGATGATTCG GTCAAAAGCA TGAAGTTGAA TGGCTATGAC 1080

TTAAGTCCAG AAAATATAAG TAGCAATAAT TGGCCCTTGT GGTCTTATGA GCATATGTAT 1140

ACATTGGGGC AGCCCAATGA GTTGGCTGCA GAATTTCTCA ATTTTGTTCT CTCGGATGAG 1200

ACCCAAGAAG GGATTGTCAA AGGATTGAAG TATATTCCGA TTAAGGAAAT GAAGGTTGAA 1260

AAAGATGCTG CCGGAACTGT GACAGTGTTG GAAGGGAGAC AATAATGAAT CAAGAAGAAT 1320

TAGCTAAGAA AATGTTGCTT CCATCAAAGA ATTCTCGTCT GGAGAAATTA GGAAAAGGTT 1380

TGACCTTTGC CTGTCTTTCT TTGATAGTCA TCCTTGTGGC CATGATTTTG GTTTTCGTAG 1440

CGCAAAAAGG CTTGTCGACC TTCTTTGTCA ATGGTGTGAA TATCTTTGAC TTTCTTTTGG 1500

GAGGAACTTG GAATCCTTCT AGTAAAGAAT TTGGTGCCCT TCCTATGATT TTGGGTTCCT 1560

TTATCGTTAC CATTCTCTCA GCCCTTATCG CAACACCCTT TGCTATTGGT GCAGCAGTTT 1620

TTATGACCGA AGTATCACCA AAAGGGGCGA AGATTTTGCA ACCAGCTATT GAACTCCTGG 1680

TTGGGATTCC TTCAGTAGTG TACGGATTTA TTGGCTTGCA AGTCGTCGTT CCCTTTGTTC 1740

GCAGTGTCTT TGGTGGGACT GGTTTTGGGA TTTTGTCAGG GATTTCCGTC CTCTTTGTCA 1800

TGATTTTGCC GACCGTAACC TTTATGACAA CGGATAGCTT GCGTGCGGTT CCTCCNTTAT 1860

TATCGTGAAG CCAGTTTCGC TATGGGA 1887

178

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 65:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 405 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65:

CTGAGGAATC AAAAGTTGAA CCACCAGTAG AACAAGCATA AGTCCCAGAA CAACCCGTGC 60

AACCTACACA AGCTGAGCAA CCAAGTACAC CAAAAGAATC ATCACAACAA GAAAATCCTA 120

AAGAAGATAG GGGAGCGGAA GAGACTCCGA AACAAGAAGA TGAACAGCCA GCAGAAGCCC 180

AAGAAATCAA GGTTGAAGAA CCAGTAGAAT CTATAGAGGA GACTGTCATT CAACCTGTTG 240

AACAACCAAA AGTGGAAACG CCTGCTGTTT AATAACTAAC GGAACCTACA GAGGAACCTA 300

AAGTTGAAGT AACTAGTATT CCCCTCACTA CTCGCTATGA GGAAGACCTT ACTTACGAAC 360

ACGGAACGCG TTGAAGTTGT TAAGGAAGGT TATAATTGGC AGTAT 405

(2) INFORMATION FOR SEQ ID NO: 66:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1542 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 66:

CTATGGGATT GGTAGTTCTT CCTAGTGCAG GGGCTGTAGA CCCAGTTGCG ACCCTAGCGC 60

TGGACTAGTC GAGAGGGTGT TGTTGAAAAT GGATGGCTAT CGCTATGTTG GTTATCTATC 120

AGGTGACATC CTCAAAACGC TTGGCTTGGA CACTGTTTTA GAAGAAACCT CAGCAAAACC 180

TGGAGAGGTG ACTGTAGTCG AAGTTGAGAC TCCTCAATCA ACAACAAATC AGGAGCAAGC 240

TAGGACAGAA AACCAAGTAG TAGAGACAGA GGAAGCTCCA AAAGAAGAAG CACCTAAAAC 300

AGAAGAAAGT CCAAAGGAAG AACCAAAATC GGAGGTAAAA CCTACTGACG ACACCCTTCC 360

TAAAGTAGAA GAGGGGAAAG AAGATTCAGC AGAACCATCT CCAGTTGAAG AAGTAGGTGG 420

AGAAGTTGAG TCAAAACCAG AGGAAAAAGT AGCAGTTAAG CCAGAAAGTC AACCATCAGA 480

CAAACCAGCT GAGGAATCAA AAGTTGAACC ACCAGTAGAA CAAGCAAAAG TCCCAGAACA 540

ACCCGTGCAA CCTACACAAG CTGAGCAACC AAGTACACCA AAAGAATCAT CACAACAAGA 600

AAATCCTAAA GAAGATAGGG GAGCGGAAGA GACACCGAAA CAAGAAGATG AACAGCCAGC 660

AGAAGCCCAA GAAATCAAGG TTGAAGAACC AGTAGAATCA AAAGAGGAGA CTGTTAATCA 720

ACCTGTTGAA CAACCAAAAG TGGAAACGCC TGCTGTAGAA AAACAAACGG AACCAACAGA 780

179

SUBSTTTUTE SHEET (RULE 26) GGAACCAAAA GTTGAAGTAA CAAGTATTCC CCAAACTACT CGCTATGAGG AAGACCTTAC 840

TAAGGAACAC GGAACGCGTG AAGTTGTTAA GGAAGGTAAG AATGGCAGTA GAACAGTTAC 900

TACTCCATAT ATCTTGAATG CGACAGATGG TACGACTACA GAAGGCACTT CGACAACTGA 960

TGAAGCTGAG ATGGAGAAAG AGGTTGTTCG TGTTGGCACG AAACCCAAAG AAAAATTAGC 1020

TCCAGTCTTA AGTTTGACAA GTGTTACAGA TAATGCAATG TTGCGTAGTG CGAGACTTAC 1080

TTATCATTTG GAAAATACAG AT GTGTTGA TGTGAAAAAA ATTCATGCTG AAATTAAAAA 1140

TGGCGATAAG GTTGTCAAAA CTATTGACTT ATCTAAAGAG AGATTATCAG ATGCTGTTGA 1200

CGGTCTTGAA CTTTATAAAG ATTATAAGAT TGTGACGAGT ATGACCTATG ATAGAGGTAA 1260

TGGTGAAGAA ACCTCTACGT TGGAAGAAAC TCCACTACGA TTAGACCTCA AGAAGGTTGA 1320

ATTGAAAAAC ATCGGCTCTA CTAATCTCGT CAAAGTAAAT GAGGATGGTA CTGAGGTGGC 1380

AAGTGACTTC TTAACAAGTA AACCTGTGGA TGTGCAGAAT TACTACCTCA AAGTAACTTC 1440

CCGTGATAAT AAAGTTGTTT CCCCTCCCAG TTGAAAAAAT TGAAGAGGTG ACTGAGGAAG 1500

GTCCACCACT TTACAAAGTC CCTGCTAAGG CCCTAATTTG AT 1542

(2) INFORMATION FOR SEQ ID NO: 67:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1321 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 67:

ATCGAATTAC TTCAACTCCA ACTTTACTCT CAATAAAAAT CAAATGTAAA AAGAGGAGCT 60

AAATTTATCT TTTTCTCCTC CTTCATCGTT CTTACTTTTG ACCATAATAA GCATTTGGTC 120

CATGTTTACG TTGGTAGTGT TTTTCTAGTA TGTACTGGGG AGCAGGTTCA ACTCTTGGAT 180

TGATTTGTTC TGTAAAGCGA TTCATCTTTG ATACTTCCTC TAGTACGACA GAGTGATAAA 240

CAGCATTCTC TGGATTTTTG CCCCAGGTGA ATGGACCGTG ATTGCGTACA ACAATTCCTG 300

GTACTTCAAC CGGGTTAAGT CCGCGATGTT CAAACTCTTC TACGATAACC AGGCCAGTAT 360

CTTTTTCATA GGCCACTTCT ACTTCGTCCT TGGTCAAACT ACGGGCGCAA GGGATTGAAC 420

CGTAGAAATA ATCTGCATGG GTTGTTCCGT AGAAAGGAAT ATCACGACCT GCCTGAGCCC 480

AAGCAACAGC TTCTGTCGAA TGGGTGTGAA CCACACTACC AATTTCTGAC CAAGCCTTAT 540

ATAATTGCAC ATGAGTTGGG AAGTCGGAAG ATGGTCTTAA ATCCCCTTAT AGGATCTTAC 600

CATCTAGATC AGTCACTACC ATGTTTTCAG GTGTCAATTC GTCATAATCC ACGCCTGATG 660

GTTTGATAAC AATGACACCG AGTTCGCGAT TGACTTCAGA TACATTCCCC CAGGTAAATT 720

TGACAAGTCC ATGTTTTGGC AATGATTGAT TGGCATCACA GACTCGTTTA CGCATAGCAT 780

TGATTACTTG ATTCATCTTA CATCAAACCT GCTTTCTTAA TGAGTGGATA GAGAAAAGCT 840

TGCGCCTCTT GAATGGCTGC GCGTGTTTCT TCTACTGTTT CACAATTTTC AGACCACATT 900

TCGATTAGGA AAGGTCCATT ATAATTGGTT TCCTTTAAAA TATCGAAAGC TTCTTCCCAT 960

TTGACACAAC CTTGCCCAAA AGGTACATCT CGGAACTGGC CCTTTGAACT TTCTGTCACT 1020

GCATAAGTAT CCTTGAGATG GAGAGTTGCG ATGGCATGAT GACCAAGATA AAACTCACTA 1080

180

SUBSTTTUTE SHEET (RULE 26) TAGATATCAT TATGCCATGC AGACACATTA CCAATATCTG GATATACAAA GAGGAAGGGA 1140

GAGTCAATCT CTTTTTCTAT AGCCAAATAT TTTTCGATGC TATTGATGAA AGGATCATCC 1200

ATAATTTCAA TAGCAAGTAC CACCTGAGCT TCTTCAGCCC AGTCACAGGC TTTTCTCAAA 1260

TTTTTGATAA AACGTTGGCG TGTCTGGGGT GACTTTTCCT CATAGTAAAC ATCGTAACCA 1320

G 1321

(2) INFORMATION FOR SEQ ID NO: 68:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1265 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 68:

TTTTTCTGTT TTTCGGAGCA AACTGGGCTC CAGCCGGTTT TGGCCTTCTT TCCTTAGCTA 60

CAGCTGGTTT AGCTGGCTCA GATTTTTCGG CTTTCTTTTC TGCACTTACT TTTGGTGCTG 120

CAGGTTTTGC TTCTACTTTC GGAGCAGCTG CAGGCTTAAA GCTGGCAGCA ATTTTTGCAG 180

CGACAGCTTC TTCCACACTT GATGAGTGGC TTTTCACATC CAAGCCCAAC TCTTTTGCAC 240

GCGCTACAAC TTCTTTACTT TCTTTTCCAA GTTCTTTTGC GATTTCGTAC AATCTTTTCT 300

TAGACAAATC ATGTCCTCCT CTTCTATTCC ATAAGAGACC TCATTTTCTT TGTAAATCCA 360

GCATCTGTTA CAGCCAAAAC CTTTCTCGAT TTCCCGACTG CTATGATTAA TTCCAGTGTT 420

GAAAACACGG TTACAATTTC TACTTGATAA TAATGACTTT TATCTTGAAT CTTCTTGGTC 480

AGATTGGGTC CAGCATCATG AGCTAGAAAG ACCAACTTGG CCTTGCCGTC TTGAATGGCC 540

TTGACCACCA ATTCTTCACC CGATATGATG CGCCCTGCTC GCTGAGCAAG CCCCAAGAGA 600

TTACTTATCT TTTGCTTATT CAAGTCCCAA CTCTCTTCTT TTCACTTTGT GATCCACATA 660

AGCGATCAAC TCGTCATAAA AGCTTTCTTC CACTTCCATG CTAAAGCTGC GGTTAAAGAC 720

CTTCTTCTTT TTCGCCTCTA GGGCTTCTGC ATTGTCTAGT TTGATATAAG CGCCGCGGCC 780

ATTGGCCTTG CCCGTAGGAT CAATAAAGAC TTGTCCTTCC TTGTTCTTGA CAATGCGGAG 840

CAAATCACGC TTATCAATCA CTTCGTTAGA CACAACAGAC TTGCGCAAAG GGATTTTTCT 900

TGTTTTCATC TTTCCCTCCT CTAGCAGCTT TTATTCTTCT ACAGTATCGT TTTCTACTTC 960

CAACTCTACT GAAGCAGCGT CTTCCATGGC TTCAAATTCG CTAGCAGACT TGATATCGAT 1020

ACGGTAACCA GTCAAGTGAG CCGCCAAGCG CACGTTTTGT CCACGACGAC CAATGGCAAG 1080

AGAAAGCTTG TTATCTGGAA CAACCACCAA GGCACGTTTG CTGTCGTTTT CATCAAAGAT 1140

AACTTGGTCA ACCTCAGCAG GAGCGATGGC ATTGTAGATA AATTCAGCTG GATCTGCTAC 1200

CCACTCGATA ACATCGATAT TTTCTTCGAT TGGTACCATG CGGTCATTTT TAGCATCGTA 1260

ACGAG 1265

(2) INFORMATION FOR SEQ ID NO: 69:

(i) SEQUENCE CHARACTERISTICS:

181

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 1305 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 69:

ATAAACCAAA GGAAGCTGAG CTCTTTAGTC CCAGCTTCTT TTTATATATA AAATTTTACC 60

CGTGAAAAGA CAGGGCCTTA GCAGACTTCT TTTTTACTTC GTTCACCCTT GCTTTTTCTT 120

TGTATGTTTG GGCGTTGGCA GTTGGTTATA CATAGCTAAA ATCAGGTCTT ATAGAAACAT 180

CTTATTATCA AGTTCTTCCA CTCAAATCAT TTCTTTGGCA CCTTTGTATG GAAACTCAAA 240

AGAAGATTGG TCAATCTTAT CTAAGACTGC TTGCACGGGT TTAACTAAAA GCGATCGTCA 300

TAAATGCCGC CAATAATCTT GCCGCGGAAG TAAAGAATAT ACTCCCCCAT CATGGAACGG 360

TAAGTCACAT CATCTAATCC TGATAATTGT TCCAAAACAA ATTCCAAATA GTTCTTACTT 420

GATGCCATTT CTAATCTTCT AGGCTCTGTT CAACGATAAC AACCGTATAG AGTTCTTGCT 480

TAACCTCGCA TCCAATTGAT TTAAAGCCCT GCTTTTCCCA AAAATGCTGA GATTGCGGAT 540

TTCCCTTAAC ATAAGCCAAA CGTGCCTTTC GAAAGTTCTT AGCAAAATAA GCTAGTGCTT 600

CTGTCACAAT ATGACTACCA ATCCCTTTCC TCTGATAGGC TTGATCAACC ATAAACAAAC 660

CAATAAAAAC AGTCTCCTCA TCAGGATATG CA AGACAAA ATCCATAACA GCCACAAGGT 720

CAAATCCATT CCAAAATCCA ACAAAAAACT TATCAGCCTT AGCTTTACCT TCAGGTAGAC 780

AAAGCATGTC CTCTTTTACA GTTGCAAAAT TTGGCTCTGG TGGACAATGC TGAAAATACA 840

GAGGATTACT TTCATATAAA GATAAAATAC TTGGAATATC CTTTTCAGTT AGTATCCTAC 900

AACTGTAATA CTTAGATAGT TGGTCAATCA TCTTTTCAAA TTCGATACTT TCTTGTGCCC 960

TGTGATTATG ACACAGGAAG ATGCACTGAT CGTCATCAGC CACATAAAAG TTCTTTCCAT 1020

CGTGCCTAAT CGTTGTCTCA AACCTTTGGA TAAAACCTTT AGCCTATACA ACTGGATTTT 1080

CCTCTCTCAA AAGTATATTC TTTTGCAGGC GAACTTCCTC AAAATCAGTC GTGTGCAACT 1140

TCAGTAGAAT ATTCATAGGC TCGGATAATC TGAGCGACAA CAGGATGGCG AACCACATCC 1200

TTGGCTGAAA AATGAACAAA GTCAATCTGA TGGATGTTCT TGAGTTTCTC TTGAGCATCA 1260

ATCAAACCGG ACTTGACATT ACGTGGCAGG TCAATCTGAC TAATA 1305

(2) INFORMATION FOR SEQ ID NO: 70:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1742 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 70:

182

SUBSTTTUTE SHEET (RULE 26) CTAATCTCCT TAAAACGTGA TCTTTTCAAG AATATTTTTA TCTAAACAAT CCAGCAAGTC 60

TTGGTAAGAA TAGACTTCGT AAGTCGGCTG GGCTTGTGTG TGATTTTCGA GGTGATGAGG 120

ATTATACCAG ATAGTGTCAA TCCCCGCATT ATTGCCACCT TGAATGTCGG CGGTTAGAGA 180

ATCTCCAATC ATCAGCGTCT TTTCTTTACT AAATCCAGCA ATTTGCTGGC CAATCTTTTC 240

ATAAAAAAGA GCATCCGGCT TTTGAGTTTG CAACTGTTCT GAGATAAAGA CTTGATTGAA 300

ATAAGGTGCT AGACCAGATT GAGCCAAACG TCCTGTCTGA ATGGCAGTAA TGCCATTTGT 360

CGCAGCATAC AAGTTATAAT CACGCTCAAT GAGGCTGTCC AAGAGATCAT GAGCGCCCGA 420

TAGTGTTTGT CCCTGCTGGG CGAGGTAAAA TTGGTAACGC TGGGCAAGAA AACTACCGTC 480

TTTTTCCTGT CCAAAATGAG CAAATAAACG AGAAAAGCGC GTGTTAACCA GCTCTTGTTT 540

ACTGATTTTC TTCAGCTCCA AGTCTTTCCA GAGAGCCTTG TTCATAGGAA CGTAATAATC 600

TTTATAAGCC GGAATATCCG CAACTCCTTC TTCTTTTAGA AGTGGAGTCA AAGCCACATC 660

CTCAGCAGCA TCAAAATCAA GAAGAGTGTG GTCGAGGTCG AAGAGTACAA ATTTGTAGAA 720

CAATTTGAGG TTTTCCTTTC TGAAAATTCA TTAAGAACAT TATATCATAA AGCACCTCAT 780

ACAATTAACT AATTTAATCA CTTAAAAAAA ATTCGAACAC TTTCTATACA ACTGACAGCT 840

CAAATCTTTC AGAATAGAAC AATACTAACT ATCGAACACC CCGTCTTCAT AAATACATAT 900

GTAATTCTAG GCCTAGAATT CCTATAAACT AAATGCTTTC ATACTCTTCC AAGTAATTGA 960

TTGCCTTAAA TTTTAATTTT TGAAGGTTTC TAAAGCTAGA ATAGCCCCAT CACAATCAGT 1020

TTTGATTGAT TCACAATTTA GAAACACTAT AGTTTCACTC CTGTTAAAAT AAAAAGGAAC 1080

TGCATAAAGC AATCCCTTTC TGATTTTGAA ATCATTTACT TAACATTTTA TAGTTGAGAT 1140

AATCAATAGC TTATCTATAA AAAGAGTTAT AGTAAAATTC CTTATTTATT GATTCCAAGC 1200

TCCGCTAACT GTATTTGAAT AACTGACAGT TCTGCACCAG CCTGAAAAAG AGCAGCTGCA 1260

TTATAGGCAC CTTCTACAAT TGGAACCCTG TTGATGATGA TACTTTTATC ACTGAAATCA 1320

GTCACCATTT TTAAGTTCAT TTTAGCAGAA CCTAGGTCAA AAAAGGCAAG TAAAGTATCT 1380

GCTGGATTTT CGGAAACAAC CCTATCTACT TGATCAAAAC TCGTTCCAAT TCCTCCGCCC 1440

TCGGTTCCTC CTACATAAGT AATCGGAACA TCTTTAGCTA CTTTACTAAT CAGTTCAACA 1500

ACACCTTCTG CAATGTGTTT GGAATGTGAA ACGATAACAA GACCAATACC AATACTTTCC 1560

ATCAAACCAC TCCAGTTTCT AAAATAGCAG TAAAGAGTAA TCCTGATGAG AATGATCCAG 1620

GATCAATATG TCCAAGAAAC CACATGCTCC TAAGACAAGA GCTAACAGAC TGGCCATCAA 1680

TAATAGTATT GTTCTTTTTT TCATCATTAC TCCTTAACTA GTGTTTAACT GATTAATTCG 1740

AT 1742

(2) INFORMATION FOR SEQ ID NO: 71:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1136 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 71:

GTGGAATGCG GGGACGCCTT GTCTAATTTT GGATCAAGCC CTGAGTTTGA CACAGGGAAA 60

183

SUBSTTTUTE SHEET (RULE 26) TGAGCTGGAC GGACTGCTAT CTCTGAAGAA ATTACTGGCA CCATTAGCCT ATCAGCCTTG 120

GATGATTATG TGGCGGCCTT GTCTCAACAG GATGTTCCCA AAGCTTTGTC TTGCTTGAAT 180

CTTCTTTTTG ACAATGGTAA GAGCATGACT CGTTTTGTGA CCGATCTTTT GCACTATTTA 240

AGAGACTTGT TAATTGTTCA AACAGGGGGA GAAAATACTC ATCATAGTTC AGTCTTTGTA 300

GAAAATTTGG CACTTCCTCA AAAAAATCTG TTTGAAATGA TTCGCTTAGC AACAGTGAAT 360

TTAGCAGATA TTAAGTCTAG TTTGCAGCCC AAGATTTATG CTGAAATGAT GACCGTCCGT 420

TTGGCGGAAA TCAAGCCCGA ACCAGCTCTA TCAGGAGCGG TTGAAAATCG AATTGCTACG 480

CTGAGACAGG AAGTTGCCCG TCTCAAACAA GAGCTTTCTA ATGCAGGTGC GGTTCCTAAA 540

CAAGTTGCAC CAGCTCCTAG TCGACCAGCT ACGGGCAAAA CAGTCTATCG TGTCGATCGC 600

AATAAAGTGC AATCTATCTT ACAAGAGGCC GTCGAAAATC CTGATTTAGC ACGTCAAAAT 660

CTAATTCGTT TGCAGAATGC CTGGGGAGAG GTAATTGAAA GTCTAGGTGG GCCGGACAAG 720

GCTCTGCTAG TTGGTTCTCA ACCGGTTGCT GCCAATGAAC ACCATGCTAT TCTTGCTTTT 780

GAGTCTAACT TCAATGCTGG TCAAACTATG AAACGAGACA ATCTCAATAC CATGTTTGGT 840

AATATCCTCA GTCAGGCGGC AGGTTTTTCA CCTGAGATTT TAGCTATTTC CATGGAGGAA 900

TGGAAAGAAG TTCGCGCAGC CTTTTCAGCC AAAGCCAAAT CTTCTCAAAC TGAAAAAGAA 960

GTAGAAGAAA GCCTGATTCC AGAAGGATTT GAATTTTTGG CTGATAAAGT GAAGGTAGAG 1020

GAAGACTAAA GAAAGATTTC ATGATACAAT AAGTTTATGA ATAAACAACA ATTTATTATT 1080

ATGGCGCTAT TTACAGCTGC TGAGACCTAT TTTTTCAATG AAGCCTGGAT GACTGG 1136

(2) INFORMATION FOR SEQ ID NO: 72:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1670 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 72:

CTGTCTCTGA AACAGTCACA TCAAGTGCCT CTGAACAANC GCCCCNCCTA GGTNGACGGT 60

ATCGATAAGC TCGATCTGTG ATTTCAGAGA AGAAATCAAG TGCTGTAACA GAAGTAAGAT 120

GTAATTGTAT GTAAAGGAGA CGTCATGTTA AATAGTATTG TAACCATTAT TTGTATTGCC 180

CTTATCGCGT TTATCTTGTT TTGGTTTTTC AAAAAGCCTG AAAAATCTGG ACAAAAAGCC 240

CAGCAAAAAA ACGGATACCA AGAGATTCGA GTGGAAGTCA TGGGAGGCTA TACTCCTGAG 300

TTGATTGTCC TCAAGAAATC AGTGCCAGCC CGCATTGTCT TTGACCGCAA GGATCCTTCA 360

CCATGTCTGG ATCAAATTGT TTTTCCAGAT TTTGGTGTAC ATGCGAACCT GCCAATGGGG 420

GAAGAGTATG TAGTGGAAAT CACGCCTGAA CAGGCTGGAG AGTTTGGCTT TGCTTGTGGT 480

ATGAACATGA TGCACGGCAA GATGATTGTA GAGTAGGTGG AGACTATGAC AGAAATTGTG 540

AAAGCAAGCT TAGAAAATGG CATTCAAAAA ATCCGTATCC GAGCTGAAAA AGGCTATCAT 600

CCAGCCCATA TCCAGCTTCA AAAGGGAATT CCAGCTGAGA TTACCTTTCA TTCGTGCTAC 660

TCCTTCAAAC TGTTATAAGG GAAATTCTGT TTGAAGAAGA AGGTATCTTG GAAGCAATCG 720

GCGTAGATGA GGAGAAAGTC ATTCGTTTTA CACCTCAAGA ATTAGGGAGA CATGAATTTT 780

184

SUBSTTTUTE SHEET (RULE 26) CTTGTGGCAT GAAGATGCAA AAGGGAAGCT ATATAGTCGT TGAGAAGACT CGAAAATCTC 840

TATCTCTCCT GCAAACGTTT TTGGATTACT AGTATCTTTA CTGTGCCTCT TGTGATTCTC 900

ATGATTGGGA TGTTGGCAGG TAGCATTAGT CATCAAGTCA TGCATTGGGG AACCTTTTTA 960

GCAACAACGC CTATTATGTT AGTTGCGGGT AAGCCATATA TCCAGAGTGC TTGGGCCAGT 1020

TTTAAAAAGC ACAATGCCAA CATGGATACC TTGGTTGCGC TGGGAACTCT AGTGGCTTAT 1080

TTCTATAGCC TAGTTGCTCT CTTTGCTGGT CTCCCTGTTT ACTTCGAAAG TGCTGGATTT 1140

ATCCTCTTTT TCGTTCTTTT GGGAGCAGTT TTTGAGGAAA AAATGAGGAA AAATACGTCC 1200

CAAGCTGTGG AGAAATTACT GGACTTGCAA GCTAAAACCG CAGAAGTCTT GAGTGATGAT 1260

AGTTATGTCC AAGTTCCTTT GGAACAAGTC AAGGTACGCG ACCTTGATTC CAGTGCGTCC 1320

CGGTGAAAAG ATTGCTGTTG ATGGTGTCGT AGTAGAAGGT GTCTCTAGTA TTGACGAATC 1380

CATGGTGACA GGTGAGAGTC TGCCTGTGGA CAAGACAGTT GGAGATACTG TCATTGGCTC 1440

AACCATCAAT CATAGTGGAA CGCTTGTCTT TAGAGCAGAA AAAGTTGGCT CAGAGACTGT 1500

TTTGGCTCAG ATTGTAGATT TTGTGAAGAA AGCTCAGACA AGTCGTGCGC CGATTCAGGA 1560

CTTGACGGAT AAGATTTCAG GGATTTTTGT CCCAGTAGTT GTCATTTTAG GAATCATGAC 1620

CTTTTGGGTT TGGTTCGTCT TGCTCAGGGA TAGTGTGGTC GTGCTTGGAG 1670

(2) INFORMATION FOR SEQ ID NO: 73:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1252 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:73:

ACAAGAACAA TTGGAACAGG TACAGGCTGT TAAAAAATCG ATTAACACAG CTAGTGAAGA 60

AGTGAAAAAC CAAGTCTTGC TACCCATGGC TGATCACTTA GTGGCTGCTA CTGAGGAAAT 120

TTTAGCGGCT AATGCCCTCG ATATGGCAGC GGCTAAGGGG AAAATCTCAG ATGTGATGTT 180

GGATCGTCTT TATTTGGATG CAGATCGTAT AGAAGCGATG GCAAGAGGAA TTCGTGAAGT 240

GGTTGCCTTA CCAGATCCAA TCGGTGAAGT TTTAGAAACA AGTCAGCTTG AAAATGGTTT 300

GGTTATCACA AAAAAACGTG TAGCTATGGG GGTCATCGGT ATTATCTATG AAAGCCGTCC 360

AAATGTGACG TCTGATGCGG CTGCTTTGAC TCTTAAGAGT GGAAATGCGG TTGTTCTTCG 420

TAGTGGTAAG GATGCCTATC AAACAACCCA TGCCATTGTC ACAGCCTTGA AGAAGGGCTT 480

GGAGACGACT ACTATTCATC CAAATGTGAT TCAACTGGTG GAGGATACTA GCCGTGAAAG 540

TAGTTATGCT ATGATGAAGG CCAAGGGCTA TCTAGACCTT CTCATTCCTC GTGGAGGAGC 600

TGGCTTGATT AATGCAGTAG TTGAGAATGC CATTGTGCCT GTTATCGAGA CAGGAACTGG 660

GATTGTCCAT GTTTATGTCG ATAAGGACGC AGATGACGAC AAGGCACTGT CTATCATCAA 720

CAATGCCAAA ACCAGTCGTC CTTCTGTCTG CAATGCCATG GAGGTTCTGC TGGTTCATGA 780

AGACAAGGCA GCAAGCTTCC TTCCTCGCTT GGAGCAAGTG CTGGTTGCAG ATCGAAAAGA 840

AGCTGGGTTG GAACCAATTC AATTCCGCCT AGATAGCAAA GCAAGCCAGT TTGTTTCAGG 900

TCAAGCTGCT CAAGCACAAG ACTTTGATAC CGAGTTTTTA GACTATATTC TAGCTGTTAA 960

185

SUBSTTTUTE SHEET (RULE 26) GGTTGTGAGC AGTTTAGAAG AAGCGGTTGC GCATATTGAA TCCACAGTAC CCATCATTCG 1020

GATGCTATTG TGACGGAAAA TGCTGAAGCT GCAGCATACT TTACAGATCA AGTGGACTCT 1080

GCAGCGGTGT ATGTTAATGC CTCAACTCGT TTCACAGATG GAGGACAATT TGGTCTTGGT 1140

TGTGAAATGG GGATTTCTAC TCAGAAATTG CACGCGCGTG GTCCAATGGG CTTGAAAGAG 1200

TTGACCAGCT ACAAGTATGT GGTTGCTGGT GATGGGCAGA TAAGGGAGTA AG 1252

(2) INFORMATION FOR SEQ ID NO: 74:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1785 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 74:

CTGCCCTAGC AGGAACGCAA GAAGGAACTG GAGAATAGGC ATTTTCAAAA TTATAACCTA 60

CACTAGCCAT CA ATCTAAT GTTGGAGTGC TAACTAGCTT ATCCTTACTA TTCAAGGATA 120

AGGCGTCTGC TCTCATTTGA TCTACAACAA TCAAAATAAT ATTTGGTTGT TTTGTCTGAA 180

CCATAAAATC TCCTTTCTAA TATGGCAAAA GAGGCACAAG AAGATATCTA CCTTTACTGC 240

ACCCCTTTCT ATATCAATCT CTCTATATAA AGCAATAACA TTCTTGTTAT GTTTTATAGA 300

ACAATGGACT AAAATATGAC TAAATCGATT AGGAAATTCA AATCATTTTC TAGTACTGTT 360

TTAGTAAGTT ACAGTGTACT ATTCCAACTT CAATAAATTA TAAACCTTTG TCTAATAACA 420

ATTTTAGTGG AGATAAGAAA TCCTACACCT AACTCATCTT ACACGTAATC TATTTCTATT 480

TTATCACAAA AAACGCAAGT AAGACCATTA ACTCAATTCA GTTTTATCTG CCATTTTCAC 540

AAATGGGAAA TAAGTCAAGA CACTAATAAT CAAACAAACA ACTGATAAGA TGATGGCACG 600

CCAATCAAAT GCTGTAGAGA AGAAACCATA TAAAATTGGA GGCATTACCC AAGTAACATT 660

TTGTGTAACA GGTGAAACAA GACCCCAGCT TGTTGCCCAG TAAGCTACCG TTGCCATGAA 720

AACCGGGCTA AGTACAAATG GTATAAATAG CAAAGGATTC AAGACAACTG GTAAACCATA 780

ATTCGATACC GGCTCACCAA TATTAAACAG AACTGGTGCT AGACCAAGTT TAGCAACTTT 840

TCGATAATGA CTGTTTCTTG AAAAAATTAA AATAGCAAGT ACTAATCCTA ATCCTCCAAA 900

CCAGACAAAC GCCCCAAAAG ACCCACTTGT CCATATATAA GGAATCGGTT CACCTTTTTG 960

GAAAGCATCC AGATTCGCTA ACATAGCAAC TCCAAATAGC CCTTCCATGA TGGGAGCCAA 1020

TACATTTCCT CCATGGAGAC CAAAAAACCA GAATAACTTA TTCAAAAAGA TCATCAGAAT 1080

AACTGCAAAG AAACTTTGAG ACAAACCTAG TAATGGCGTT TGTAACACCT TGTAAACCCA 1140

ATCAATCAAT AAGTCATTGC TAAGTAAATG GAAAACATAA GTCAAGATGG CTACTATATA 1200

CATCGCCATA AATCCTGGAA TGATAGAAGT GAACGGCTTA GCAATCGCAG GGGGAACTGA 1260

ATCTGGTAAC TTGATTACCC AGTTCTTTTT CATTACTTTA CAGAAAATAA TAGAGGCTAA 1320

AAATCCAATC ATCATGGCTG TAAAGTAGCC TCTGGCATTA ATATGGTTTC CTGGAATCAC 1380

ATTCCCAATA GTTACCATCA GATTTTTACC ATCAAATGCT AGATTATCAA TTCCATGTTA 1440

AGATTTGATC TAATTTCACA TCTCCTACAT TTGCCAAAGG GAAACTCTTT GTAACTGTAC 1500

TTCCAATCGA AATGACAAAC GAAGCAAGTG ATACCAAACC AGCAGAAACT GTATCAACCT 1560

186

UBSTTTUTE SHEET (RULE 26) TGTAAATCTT AGCGATATTC ACTCCCAAGC AATAGATGAA CAACAAGGAA ACAATTGGTA 1620

TACTTCCCTT GAATACCAAA TTATTGATGT CAACAAGCCA CTGAAAGGTT TTCGTAATAC 1680

TTCCTAGGTG AAATTGTTGT GGTAAATCCA CTAGAAAAGC ATTTAATAAC AAAGCAATGG 1740

AACCTGTCAT AATAACAGGC ATAGTCCCCA CAAATGAATC ACGTT 1785

(2) INFORMATION FOR SEQ ID NO: 75:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1386 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 75:

ATCGAATTTC ATTTCTATTT CCTATTCCAT TTTTATTCAA AAAATCAAAA AGCAAACTAG 60

AAAGCTGGTC GCTGGTGGTT CAAAACACTG TTTTGAGATT GTCAATAGAA CTGACAAACC 120

CTGTAATATA CCTGCATATA TACATACGAC AAGGCGATAC TACCCTAGTT TGAAGAGATT 180

TTCGAAGAGT ATTCATTTTT GTCTTTTACT TATTATACCA TATTCACATA AAAAAACGAA 240

CATTCTTATC CTAAAAAATG CTCATTTTTC TTAAATTATC AATCTAAATC TGGTTTATAG 300

AAGGAACGAT TATCCATAGC GAAGATTTTA TTGGTCATCT CTCCTTTATC CACCAAAGCC 360

AGAGCTGTTG ACATCATCAT CATGCTTGCA TCCAGATTGT CAATCATATG GATAATCTCT 420

GCCTCCATAA TACGTGGACG GACTGGAATT TCCATATTCA AGCAAGCCGT GGTGGACTTG 480

AGGATGACAT GACGAAGCAA AACGACTTCT TCCTTGGTAT CATCGATGCC GAGTTCCATA 540

ACTGTCTTGG TAATTTCGCT ATCAATGAGA GCGATATGTC CAAGAAGATT ACCTCGCACT 600

GTGTACTCTG TCTGGTCTGG CCCCGTCAAC TCGATAACCT TAGCTAAGTC ATGCAGCATA 660

ATCCCCGCAT AGAGCAGGCT CTTATTGAGC TGAGGATAAA CTTCGCTAAT AGCGTCTGCC 720

AAACGTACCA TGGTCGCCGT ATGATAAGCC AACCCCGTTT CAAAGGCATG GTGGTTGGTC 780

TTGGCGGCTG GATAGGAGTA GAATTCCTTA TCATACTTGG TGTAGAGATT TCGGACAATC 840

CGTTGCCAGA CAGGATTTTC AATTTTGAAA ATCATTTGCG ACATGTAGTC ACGAATTTCC 900

TTGACATCAA CTGGTGACTT GACCTTGAAA TCAGCTGGGT CATTGGGTTC ACCAGCTTGA 960

GGCAGGCGGA GAGTAATTTG ATTGACTTGA GGGGTATTGT TAT AACTTC TCGGCGTCCT 1020

TTCATGTGGA CAACCTTACC TGCGGTAAAG GCCTCAATGT TATGAGGTTG GGCATCCCAG 1080

AGCTTCCCAT CAATCTCGCC ACTATCATCT TGGAAGGTAA AGGCTAGGTA GTTTTTCCCA 1140

GCTCGAGTTT GCCTCAGGTC AGCTGATTTG ATTAGGTAAA AGCCTTCAAA TAACTCATCT 1200

TTTTTCATGT GACTAATCTT CATATTCTTC CTCATTTTCT TGAAAATGGA GTAGATCAAG 1260

CGCAGGCTCA CCTTCTGACA ACTCAATGTG ACGGAGCGTC CGCTCGATAG CTATGGTACG 1320

ACGGTTTAAT AATTCGATCA ATATTGCCAG AGGCATGTTG GAGATGTTTT TGTGCCTTGA 1380

CCAGAA 1386

(2) INFORMATION FOR SEQ ID NO: 76:

187

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1167 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 76:

CTCAGATTAC AGAGGACAAT CAACTGGTTC ATTTTCGTTT CCAGTTTCAA AAAGGCTTAG 60

AAAGGGAGTT CATCTATCGT GTGGAAAAAG AAAAAAGTTA AGGCAGGTGT TCTCCTCTAC 120

GCAGTCACCA TAGCAGCCAT CTTTAGTCTT TTGTTGCAAT TTTATTTGAA CCGACAAGTC 180

GCCCACTATC AAGACTATGC TTTGAATAAA GAAAAATTGG TTGCTTTTGC TATGGCTAAA 240

CGAACCAAAG ATAAGGTTGA GCAAGAAAGT GGGGAACAGG TTTTTAATCT AGGTCAGGTA 300

AGCTATCAAA ACAAGAAAAC TGGCTTAGTG ACGAGGGTTC GTACGGATAA GAGCCAATAT 360

GAGTTTCTGT TTCCTTCAGT CAAAATCAAA GAAGAGAAAA GAGATAAAAA GGAAGAGGTA 420

GCGACCGATT CAAGCGAAAA AGTGGAGAAG AAAAAATCAG AAGAGAAGCC TGAAAAGAAA 480

GAGAATTCCT AGTCAATTCA ACTATAATGC GTTGAATCCA GAATAGTCCA CTGTAGTTTC 540

TAGAAAATTG CTGGAAATGG ATGTTAAGCT CCAATTCATT TGTTTATATC TTATTTCAGT 600

CCACTATACT TTGTGCTAAA TTAAAGATAT GAAACATGAT TTTAACCACA AAGCAGAAAC 660

TTTCGATTTC CCTAAAAATA TCTTCCTCGC AAACTTGGTA TGTCAAGCAG CCGAGAAACA 720

GATTGATCTT CTATCAGACA AAGAAATTTT AGATTTCGGT GGTGGCACGG GTCTATTAGC 780

CTTGCCCCTA ACCCCTAGCC AAGCAGGCTA AGTCAGTCAC TCTTGTAGAC ATTTCTGAGA 840

AAATGTTGGA GCAAGCTCGT TTGAAAGTGG AGCAGCAAGC AATCAAGAAT ATCCAGTTTT 900

TGGAGCAAGA TTTACCGAAA AATCCCTTGG AGAAAGAGTT TGATTGCCTT GCTGTTAGTC 960

GGGTTCTTCA TCATATGCCT GATTTGGATG CGGCTCTCTC ACTGTTTCAT CAACATTTGA 1020

AGGAAGATGG GAAACTCATC ATTGCTGATT TTACCAAGAC AGAAGCTAAT CATCATGGAT 1080

TTGATTTAGC TGAACTGGAA AACAAGCTAA TTGAGCATGG GTTTTTCATC TGTGCATAGT 1140

CAGATNCTCT ATAGCGCTGA AGANCTG 1167

(2) INFORMATION FOR SEQ ID NO: 77:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 916 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 77:

TCTCCCAACA TATAATTTCC GTTTTCCAAT CCCCCAGCTG TCATACAGTC TGTGATAAGA 60 GCGATGTTTT CTGTTCCTTT TTGTTTGATA AGAATTTCGC AAGCCTTTGG ATCTACGTGG 120

188

SUBSTTTUTE SHEET (RULE 26) TGACCATCAC AGATCAACTC TGCATAGGTA TGTGGCAATT GGTACATGGC TCCAACCATA 180

CCCAATTCAC GGTGAGTCAA CCCACGCATT CCATTGTAGG CATGCACCCA AACACTCGCT 240

CCAGCATCGA CTGCTTTTTT GGCTTCATCA AAAGTCGCGT TTGAATGTCC AAGAGCAACC 300

GTCACACCTT CGCCCGTAAC TGTACGAACA AAGTCTTCCA CCCCATCACG TTCTGGTGCA 360

ATCGAATTTT ATTAAGCAAG CCATTTGCCG CTTTTTGCCA AGAATGAAAC TCCTCAACAC 420

CCGGGTCTCT CATATAAGTT GGATTTTGTG CCCCCTTAAA AGTTTCTGTG AAATATGGAC 480

CTTCATAATA AATCCCACGA ATCTTAGCAC CTGTTGCTTC TTTATAATGG TTTCCAAGAT 540

TTTCAGTGAC TGCAAGCAAT TGCTCATAAG TGGCTGTTAA AGTTGTGGGT AAGAAACTGG 600

TAACACCGGT ACTAAGAAGT CCTTCACTCA TAGTATGCAA TGTACCTTCA ATGTTGTTGT 660

CCATCACATC TACACCTGCA TATCCATGAA TATGAGTATC CACAAGACCT GGGGCAATGC 720

TATAACCTGT ATAGTCAATC ACCTCAGCCC CTTCAGGAAT CTGCTCTACA TGTTTCCCAA 780

ACTTGCCGTC CACAAGTTCC AAGTAACCAC CTCGACAAAT CCGTGTGGGT AGAAAAACTG 840

ATCCGCTTTA ATATAGTTAG GCATAATGTT AACCTCCTTA AAAGATTGAT TCTACAATTT 900

ATTATGTCAA TTCGAT 916

(2) INFORMATION FOR SEQ ID NO: 78:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 786 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 78:

CTGGATTAAA ACGAGGCAGT TTCAGACTAA TATCCAAGTC GTAAGAAATG CCTGAAATAA 60

GCTTTTCTAA ATTGTCCAAA GCTTGCGGGA AAACGCTCTT GGAATAGTTT CTCTAAAGAA 120

CTTGCTGATA TAAAGACATC TTGTCTCGAA CGCAAGGGAA CTTCTCTGAG CGGTAGATTT 180

TCTTTAATCG CTGTTAAAAC TTGAAGAACT TCTCTATCCC TGCTTTCAAA AGCGTTGACC 240

CGATAAAGAG GTAAGATAGG ATGATGAAAT TCGCTTGCTA GTGTTTCTGG ATAAACCCCT 300

ATATAGTAAT CACAGCCTAG TTCTAACGAC TCAACTCTAT CAAAATAAGG CACAATGACC 360

GCGATATCCT CCAGGTACTG GGACAGGACT GACCAAGTTT TCTCCCCCTG CATCTTGGCT 420

GTCGAAAGCT TCATCAACTG CTGATAGCCC ACACTAGATA GAGCTAAAAA GCGCAAATTC 480

ACTTCCTGAT CATCTACAAA CACTGTCATT TCAAGCCCTA GCAAAGGATG AATGCCGTAT 540

TTTTTTGTAA TCTCTAGAAA GTCGAAAGCG CCATAAAGAT TGTCAATATC CATCATAGCC 600

AAATGAGTGT AGCCGTATTC TTTAGCTGCT CTCACATACT TTTCGATCGA AATGACGCTT 660

TCCATAAAAC TATAGACTGT TTTTGTATCT AGTTGTGCGA TCAATTTACA CTTCTCCTCT 720

ATCCTTCTCA CTATATTATA CCATTTTCAC CTATAAATGG CTTCTCTTGA GAAAAATTTC 780

GATCAG 786

(2) INFORMATION FOR SEQ ID NO: 79:

189

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1213 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 79:

CACTTTCAGC TTCTTCTCTT TTTGAACGGT TATAAACACG AATCAGATTC CCTATTTCTT 60

GCGATTTATG TGATTCCTTA TTTTCCAATC TAAAGTATAG TGAAATGAAA TAAAACATGC 120

GCAAATCGAT TAAGGAATTT AATCTAATTT CTAACAATGT CTTAGAAATC AAAGTGTACT 180

ATTTTAACTT CAATGCACTA AACATCTAAT ACTCAATAAA AATCAAAGAG CAAACTAGGA 240

AACTAGCCGC AGGTGGCTCA AAACACTGTT TTGAGGTTGT AGATGAAACT GACGAAGTCA 300

GTAACCATAC ATACGGCAAG GCGACGCTGA CGTGGTTTGA AGAGATTTTC GAAGAGTAGC 360

AAAATGGAAA AAGGAGTGAG TGAAGCACAT CGCCTCCCCA CTCCTTTTTC TGTTTTTAGG 420

CTGTTTTTTC AACCTTCAAG ATTTTTACAT CATAGCTACC AACAGGCGTT TCAATGGTTG 480

CTGTATCACC TGTTTTCTTG CCAATCAAGG CCTGCCCAAT TGGGCTTTCA TTTGAAACCT 540

TACCTGCAAA GGCATCCGCA CCAGCTGAAC CTACGATAAT ATAAACTTCT TCTTCGTCCT 600

CACCAATTTC TTGGATGGTG ACTGTTTTAC CAATCGCTAC TTCGTCCTGG GCAACTGCGT 660

CGCTATTGAC GATTTCAGCA TAGCGGATTT TTGTTTCTAA GCTAGAGATT TGTCCTTCGA 720

CAAAGGCTTG TTCATCCTTA GCTGCTTCGT ACTCACTGTT TTCTGAAAGG TCACCGTATG 780

AACGGGCAAT CTTAATGCGT TCTACCACTT CTGGTCGACG AAACCAATTT CAATTCTTCT 840

AATTCTTTTT CAAGTTTTTC CTTTTCCTCA AGGGTCATAG GATATGTTTT TTCTGCCATT 900

TTTCTCAACT TTCTTCTGAT AATATTTTCT AAAGAAAATT ATGTGAAGTA TCACATAATT 960

TTAGTTTGTT TAGTTTAATT TGCTGTTGAC ATGTTCAGCG ACATTGCGGT CGTGGTCTTC 1020

TTGATTGTTA GCATAGTAAA CCTTGCCTTC TGTGACATCT GCTACAAAGT AAAAGTTATC 1080

GCTCTTAGTT TGATTGATGC TTGACTCAAT CCGCATCCAA GACTTGGACT ATCGACTGGA 1140

CCAGGCATGA GACCTACATT TTTATAAACA TTATAAGGTG AATCAATGTT GGTATCAATC 1200

GCAACATCCT CAG 1213

(2) INFORMATION FOR SEQ ID NO: 80:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1173 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 80:

190

SUBSTTTUTE SHEET (RULE 26) TGCGGCTGAG TTGGGAATTC CTATCGTTAA TAAGCGTGTA TCGGTGACAC CTATTTCTCT 60

GATTGGGGCA GCGACAGATG CGACGGACTA CTGGTTCTGG CAAAAGCGCT TGATAAGGCT 120

GCGAAAGAGA TTGGTGTGGA CTTTATTGGT GGTCTTTCTG CCTTAGAACA AAAAGGTTAT 180

CAAAAGGGAG ATGAGATTCT CATCAATTCC ATTCCTCGCG CTTTGACTGA GACGGATAAG 240

GTCTGCTCGT CAGTCAATAT CGGCTCAACC AAGTCTGGTA TTAATATGAC GGCTGTGGCA 300

GATATGGGAC GAATTTATCA AGGAAACGGC AAATCTTTCA GATATGGGAG CGGCCAAGTT 360

GGTTGTATTC GCTAATGCTG TTGAGGACAA TCCATTTATG GCGGGTGCCT TTCATGGTGT 420

TGGGGAAGCA GATGTTATCA TCAATGTCGG AGTTTCTGGT CCTGGTGTGG TGAAACGTGC 480

TTTGGAAAAA GTTCGTGGAC AGAGCTTTGA TGTTAGTAAC CCGAAAACCA GTTAAGAAAA 540

CTGCCTTTTA AAATCACTCC GTATCCGGTC CAATTGGTTT GGTCAAATGC CCAGTGAGAG 600

ACTGGGTGTG GAGTTTGGTA TTGTGGACTT GAGTTTGGCA CCAACCCCTG CGGTTGGAGA 660

CTCTGTGGCA CGTGTCCTTG AGGAAATGGG GCTAGAAACA GTTGGCACGC ATGGAACGAC 720

AGCTGCCTTG GCCCTCTTGA ACGACCAAGT TAAAAAGGGT GGAGTGATGG CCTGTAACCA 780

GGTCGGTGGT CTATCTGGTG CCTTTATCCC TGTTTCTGAG GATGAAGGAA TGATTGCTGC 840

AGTGCAAAAT GGCTCTCTTA ATTTAGAAAA ACTAGAAGCT ATGACGGCTA TCTGTTCTTG 900

TTGGATTGGA TATGATTGCC ATCCCAGAAG ATACGCCTGC TGAAACTATT GCGGCTATGA 960

TTGCGGATGA AGCAGCAATC GGTGTTATCA ACATGAAAAC AACAGCTGTT CGTATCATTC 1020

CCAAAGGAAG AGAAGGCGAT ATGATTGAGT TTGGTGGTCT ATTAGGAACT GCACCCGTTA 1080

TGAAGGTTAA TGGGGCTTCG TCTGTCGACT TCATCTCTCG CGGTGGACAA ATCCCAGCAC 1140

CAATTCATAG TTTTAAAAAT TAAGAAAATA GGA 1173

(2) INFORMATION FOR SEQ ID NO: 81:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1209 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 81:

TCGGAATCTG AGCTAGTGTA GCTTCCTTAA TCTTATCTGA TAAGATAGCT GTCATATCAG 60

ACTCAATCAT TTCCTGGAGC AATCAACATT GACTCGTATA TTCCGACTAG CGACCTCGCG 120

TGCCACAGAC TTGGTAAAGC CAATCAAGCC AGCCTTAGAA GCAGCATAGT TAGCTTGACC 180

AATATTCCCC ATCAAACCAA CAACACTAGA CATATTAATG ATAGCACCTT CTCTGGCTTT 240

CATCATCGGT TTCAAGACTG ATTGTGTCAT ATTAAAGGCA CCAGTCAGAT TGACCTTGAG 300

CACTTTTTCA AAATCTGCTT CTGTCATCTT GAGCATAAGA GTATCTTGGG TAATCCCTGC 360

ATTGTTGACC AAAACATCTA CTGAACCCAG TTCTGCAATA GCTTGATCAA TCATACGCTT 420

AGCGTCTGCA AAATCTGATA CATCTCCTGA AATGGGAACC ACCTTGATAC CATAGTTTGA 480

AAACTCAGCG AGCAATTCTT CTGAGATTGC CCCACGACTG TTTAAGACAA TGTTGGCTCC 540

TGCTTGAGCA AACTTGTGGG CGATGGCAAG ACCAATTCCA CGACTCGAAC CTGTAATAAA 600

GATATTTTTA TGTTCTAGTT TCATTTTTTT CCTTTCAAAA CTTCTACTTA TTTTAGTCTA 660

191

SUBSTTTUTE SHEET (RULE 26) TTTTTCTAAA AGTGCTACTA AACTCGCTTG ATCTTCCACA TGAGCTAAGT GAGCAGTTTG 720

ATCAATTTTT TTAACAAAAC CTGACAAGAC TTTCCCCGGT CCAATCTCGA ATAAAGTTGC 780

TTATGCCTGC TTCTTGCATG ACCCCAATAC TTTCATAGAA ACGAACGGGT TCCTTGACCT 840

GACGCGTCAA GAGCTGAGCA ATGTCCTCTT TTTGCATCAC AGCAGCTTCT GTATTGCCGA 900

CTAGGGGACA AGTAAAATCT GAAAAACTTA CCTGAGCTAG AGTTTCAGCT AGTTTCTGGC 960

TAGCAGGCTC AAGGAGAGCG GTGTGAAAGG GACCTGACAC CTTAAGAGGA ATCAAGCGTT 1020

TGGCACCTGC TTCTTGCAAA AGTTCAACCG CTCGATCAAC TGCAACCACT TCTCCAGCAA 1080

TGACGATTTG TGCAGGTGTG TTATAGTTGG CTGGAGTAAC CACTCCAAGT TCCAGAAGCT 1140

TTTTGACAGG CTTCTTCAAT GACCTCTACT GGCGTATTGA GAACTGCTAC CATCTTGCCA 1200

AGTTCAGCA 1209

(2) INFORMATION FOR SEQ ID NO: 82:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 813 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 82:

ATGACACGTC TGTTCTCTCA AGCAGAAATG GCAGAGTAAC AAGCTCGATA TTGAGGTAGC 60

CGATAAAGAA TTGGCTGAAT TTGAAGCTCA GATTAAACAG GAAGTGGAAG CTCCAACTTG 120

TAGTGAGTCC TCAGGTTGAA GAAGAGCCTC AGCTCATCCA GTTGGCCCAA TGTATGAAGA 180

ACCAGAAGTA AATCCAGTGC ATCCGACAGG TCCAACACCA GCTACAGAAA CTGTTGATTC 240

AATACCGGGA TTTGAAGCAC CGCAAGAATC TGTTACAATT TTATAAGAAA TATTCTGAGA 300

ACAATATCTT ATCCTTATAT TTCCAGCGAG CAGGAAATGG TGTGAGTCCT GCATTCCCTA 360

TCGATAAGAT TATCCTCTCA AACTATCAAG TCTGAATCTA GTAAGATTTG ACGTTCCCCA 420

CGTTACGGGA TAAGAGAGAG AAAGACTAAA TCTTTTTCCG AATAAAGGTG GTACCACGAT 480

TTTCGTCCTT TTTGGAAGTC GTGGTTTTTA ATTTGTTATT ATTTATAAAG GAGATACCAT 540

GAAACTCAAA GACACCCTTA ATCTTGGGAA AACTGAATTC CCAATGCGTG CAGGCCTTCC 600

TACCAAAGAG CCAGTTTGGC AAAAGGAATG GGAAGATGCA AAACTTTATC AACGTCGTCA 660

AGAATTGAAC CAAGGAAAAC CTCATTTCAC CTTGCATGAT GGCCCTCCAT ACGCTAACGG 720

AAATATCCAC GTTGGACATG CTATGAACAA GATTTCAAAA GATATCATTG TTCGTTCTAA 780

GTCTATGTCA GGATTTTACG CGCCATTTAT TCC 813

(2) INFORMATION FOR SEQ ID NO: 83:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 953 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

192

SUBSTTTUTE SHEET (RULE 26) (D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 83:

ATCGAATTAT TTTGAAACAA GGTGGATCAG CTATTTTGGC CTTGATTAGT ATTTTACTCT 60

TTAAATACAC TTGAAGGTCG ATTCTAATCT CGCTAATCCT TTTTAATCCA GAATAAGGGA 120 AATATGTTAT ACTTGTTTTT AAGAAAAAAG TTTCATTGAA TTGGTTTTGA GGAGTTAGAA - 180

ATGAAAGTAT TAGTGACAGG TTTTGAGCCC TTTTGAGGCC ATTAAAGGTT TACCAGCTGA 240

AATCCATGGT GCTGAGGTCC GTTGGCTAGA GGTGCCGACA GTTTTTCACA AATCTGCTCA 300

AGTATTGGAA GAAGAGATGA ATCGTTATCA ACCTGACTTT GTCCTTTGTA TTGGGCAAGC 360

TGGTGGAAGA ACTAGTTTGA CACCTGAACG AGTGGCCATT AATCAAGACG ATGCACGTAC 420

TTCTGATAAC GAAGATAATC AACCGATTGA CCGTCCCATT CGCCCAGATG GTGCTTCGGC 480

CTACTTTAGT AGTTTGCCGA TTAAAGCGAT GGTTCAAGCT ATAAAAAAGA AGGATTACCG 540

GCCTCTGTTT CCAATACGGC AGGGACTTTT GTCTGCAGCC ATTTGATGTA TCAGGCTCTC 600

TATTTGGTAG AAAAGAAATT CCCATATGTT AAGGCAGGTT TTATGCATAT TCCTTATATG 660

ATGGAACAGG TGGTGAACAG ACCGACTACT CCAACTATGA GTTTAGTGGA TATTCGGCGA 720

GGGATAGAAG CAGCAATCGG CGCTATGATA GAACATGGAG ATCAGGAACT CAAGTTGGTA 780

GGCGGAGAAA TTCATTGATA GAAAAAAGCT TGAGGGGAAA ACCTTCAAGC TTTTGGACGT 840

TTTCGAGCCA ATACTGCTCG GTAAAACATA ATTTTAGTGC ATTGGATATA AGGTAGGAGT 900

GAAAAACTAG CAATGCCAAA GGTAATCCAA TTGAGGAAGT ACCAAGGAAG AAG 953

(2) INFORMATION FOR SEQ ID NO: 84:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1060 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 84:

CTACTTGAAA CAGAACTGAA ATTATACCCA CTACCTCCCT GATTATCTTC AATGCTTACG 60

TCTAAATAAA CTTCCCCACT ATTATTTAGC TTAGCAACAA CTGTTATAGT AAAATAACAT 120

AAAATTCACA TAAATAGATT AGGGAAATCA AAGCAACTTC TAGGAATGTT TTAGCAGTCA 180

CAGTGTACTT TCCCAGCATC AAGCCACTAT AACTCTGCAC ATAAAAATGG AGAAGATGGC 240

CATCCTCTTC TCCAAATATT AACTTCTTTA CAAACCAACT ATAGTTGACA AAGAACCTAA 300

AATCAATTGA TAACACGAGG TCAGGTCGGT CAACTCTTTC AACTGAAGCC CTGTCAACTC 360

TTCCCATTTA TCAATCTTGT ATTGGAGAGA ATTGCGGTGC AGATAGAGTT GCTGGGCTGT 420

TTAAGTGAGA ACAGCACTAT TTTCCCAAAG AGAGAGAATG ATTTCCTGAA TCTGATCTTG 480

ATCCAAAATC ATCTGGTGTA GACATTCCTT GATTGGCTTC AAGTCCACGA GTCTTTCTCC 540

CAGACTCCAA AGATAGAGCT GAGAAAAAGT ATGAACACCT TGGTGACCCT GACGCCACCA 600

193

SUBSTTTUTE SHEET (RULE 26) TGTCTTGAAC AAATCCCGCT CAGCTTTGAT TAAGTCTGAT AGGGCTTGAT GTCCCGTCTG 660

AGACCAAACC TGACCCAACA TGATAGAAAG ACGAAGTCCA AAGTCATACT CAACCGCTTC 720

AATCGTATCA CTTAAAATAT CTCTTACAGA AGTGTATTTG TCTTGTTGAA GCACGAAAAC 780

ATAATCCTGA GATCCGACCT GTAGCACTGT CTGACAATTC GGAAAAAGAG TCCGCATCAT 840

ATCTAGCCAA GAAGCCAGAT TTTCCTGCTG AAAATAAGAA AGATGGCAAT AAACCAACTG 900

AATCTTTTTA AAAACTTGCG GTGCCTGTCC CTTGCCTTCA ACCAGATAGG AATACCAAGG 960

GTTTAGCGAA CGAACCTGCT CCTGCTGGGT CAAAAGGGCA ACCAACTGCT TTTCACGCTC 1020

GCTGAGCCCA GCTTCCTCCA GCAAAATCCA CTGCTGAGAG 1060

(2) INFORMATION FOR SEQ ID NO: 85:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 895 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 85:

ATTTTAGACT TTGATGACAA TCCTCAGGCG GTTATCATGC CCAATCACGA GGGGCTGGAA 60

TTGCAGTTGC CAAAGAAGTG TGTTTATGCA TTTTTAGGTG AGGAGATCTG ACCGCTATGC 120

AAGGGAAGTA GGGGCGGATT GTGTCGGCGA ATTCGTTTCT GCTACCAAGA CCTATCCAGT 180

CTCTTTCATC AACTACAAGG GTGAGGAGGT CTGTCTGGAT CAGGCTCCTG CTGGCTCCGC 240

TCCAGCAGCC CAGTTTATGG ATGGGTTGAT TGGCTATGGT GTGGAGCAGC TTATCTCTAC 300

TGGGACCTGT GGTGTCCTAG CTGATATAGA GGAAAATGCC TTTCTAGTCC CTGTTCGCGC 360

TTTGCGAGAT GAGGGAGCCA GTTACCACTA TGTGGCACCT TGTCGTTATA TGGAAATGCA 420

GCCAGAGGCT ATTGCTGCTA TTGAGGAAGT TTTGGAAGAC AGAGGGATTC CTTATGAAGA 480

AGTCATGACC TGGACGACAG ACGGTTTTTA CCGAGAAACG GCTGAAAAGG TGGCTTATCG 540

TAAGGAAGAA GGCTGTGCTG TTGTGGAGAT GGAGTGTTCT GCTCTTGCGG CAGTAGCTCA 600

ATTGCGTGGG GTTCTCTGGG GTGAATTGTT GTTCACAGCA AATTCTCTAG CGGACTTGGA 660

CCAGTACAAC AGTCGTGACT GGGGCTCGGA ACCTTTTAAT AAGGCGCTAA AACTGAGTTT 720

AGCAAGTGTC CACCACCTTT AGTTGTACTG GCAAAGGATT TGTTTTATCA TAAAATGTCT 780

AGCTCATACT TTTCAAAAAT ATGTTTAAAC GAAGTCACCT TCCTCTTGTC CTAAGCATGT 840

TTGAAGTTGG GAAAAATCTT TAAAATCAGA AAAACGTATC ATATCAGGTT GATGA 895

(2) INFORMATION FOR SEQ ID NO: 86:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 645 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

194

UBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 86:

AGGGCTGTCA AGCTTGGTTA GAACGTTTAG AAAAGGAGAG TTAAGGTGGA AAATCTTACG 60

AATTTTTACG AAAAGTATCG TGTCTATCTG ACTCGTCCAC GTTTAGAGCT TTTGGCAGTA 120

GTTACCATTG TTTTANGNGC TGTACTCGTC TTTTTTCTAA ATATTCCAGG AAAAGGTGTC 180

TTAAAACTCG ATAATGGAAC GATTGTTTAT GATGGCAGTC TTGTCCGTGG TAAAATGAAT 240

GGCCAAGGTA CCATTACCTT CCAAAATGGA GACCAATATA CAGGTGGCTT CAACAATGGA 300

GCCTTCAACG GAAAAGGTAC CTTTCAATCT AAAGAAGGCT GGACCTACGA AGGTGATTTT 360

GTAAATGGTC AGGCTGAAGG AAAAGGGAAA CTAACAACAG AACAAGAAGT CGTTTATGAA 420

GGAACTTTTA AACAAGGCGT TTTTCAACAA AAATAAAGCC TCCTTATCAA AGGAGGTATT 480

ATTAGAATTA CAAGGTAAGC GTTTACCTGT AAATCCCTTT CTTTCCAAAT CCCTCTTCCA 540

AGCAAGTTTG TGAAATAAAA AATATTTGAA ATAAATTTCA CAAACTTCAA AGATAAAACC 600

TGATAAGAAA AGAAAATGAG AAAAGTTTCG CAAGAGTTTA AAAAT 645

(2) INFORMATION FOR SEQ ID NO: 87:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 572 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 87:

GAGATCTGTC TTGACACCAA AAGTGTGGAG TACGCCAGCT AATTCAACGG CGATATAACC 60

AGCGCCTAGA ATCGCAATTG ACTCTGGAAG TTCTTCCCAG GCAAATACAT CATCAGAAGA 120

GCCACCTAGC TCAGCACCAG GAATATTAGG AATACTTGGA TGGGCACCTG TAGCAATCAC 180

GATATGTCTA GCACGAATCA GTTCACCATT TACGCTTACA GTATGAGAAT CTACAAATTC 240

AGCATGACCT TCAATCAAGT CTACACCGTT GCGTTTAAAA CTACCATCAT AGAGAAGAAC 300

GAGCGCGATC AATGTAGGCT TCACGATTGC GACGTAGGGT TGCAAAGTTA AAGTTAAGAT 360

CAGTAGTCTC AAAGCCGTAG TCTCCTCCAA ATTGATGGAA AGTCTCAGCG ATTTGCGCCC 420

CGCTACCACA TGATTCTTTT AGGAACACAA CCGACGTTGA CACAGGTTCC ACCTAATTTC 480

TTTTCCTCAA TAACGGCTGC TTTGGCTCCA TGTTCCCAGC ACGGTTCATG GTAGCGATCC 540

TCCGCTACCT CCACGATAGC AATGATATCA TA 572

(2) INFORMATION FOR SEQ ID NO: 88:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 49 amino acids

195

SUBSTTTUTE SHEET (RULE 26) (B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 88:

Val Gly Asp Asp Thr Trp Leu Phe Asp Pro Ala Lys Asp Pro Val lie

1 5 10 15

Met lie Leu Pro Glu Thr Phe Phe Leu His Ala Phe Leu Leu Phe Phe

20 25 30

Ala Leu Tyr Glu Asn Phe Phe Gly Tyr Leu Tyr Leu Lys Ser Arg Arg

35 40 45

Lys

(2) INFORMATION FOR SEQ ID NO: 89:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 47 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 89:

Val Gin Asp Phe Tyr Thr Ser lie Asp Val Leu Ala Glu Leu Asp Asn

1 5 10 15

Gly Thr Gin Val lie lie Glu lie Gin Val His His Gin Asn Phe Ser

20 25 30

Ser lie Thr Cys Gly Leu Thr Cys Ala Val Arg Leu lie Lys Ser 35 40 45

(2) INFORMATION FOR SEQ ID NO: 90:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 67 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

196

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 90:

Val Phe Ala Tyr Phe Thr Lys Pro Leu Gly lie Lys Leu Pro Pro Tyr

1 5 10 15

Phe Asp lie Val His Phe Asp Gin Ala Ala Ala lie Phe Asn Lys Tyr

20 25 30

Pro Leu Lys Phe Val Asn Cys Val Asn Ser lie Gly Asn Gly Leu Tyr

35 40 45 lie Glu Asp Glu Ser Val Val lie Arg Pro Lys Asn Gly Phe Gly Gly

50 55 60

He Gly Gly 65

(2) INFORMATION FOR SEQ ID NO: 91:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 97 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 91:

Val Glu Glu Val Glu Val Ala Glu Val Lys Asn Ala Arg Val Ser Leu

1 5 10 15

Thr Gly Glu Lys Thr Lys Pro Met Lys Leu Ala Glu Val Thr Ser He

20 25 30

Asn Val Asn Arg Thr Lys Thr Glu Met Glu Glu Phe Asn Arg Val Leu

35 40 45

Gly Gly Gly Val Val Pro Gly Lys Ser Arg Pro His Arg Trp Gly Ser

50 55 60

Trp Asp Trp Glu He Asn Ser Ser Pro Thr Ser Leu Asn Pro Val Val 65 70 75 80

Pro Ser Gly Asp Ser Ser Leu Cys Gin Trp Gly Gly Val Cys Pro Ala

85 90 95

Asp

197

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 92:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 75 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 92:

Val Asp Val Phe Tyr Asp Gly Gin Thr Phe Thr He Leu Glu Asn Pro

1 5 10 15

Val He Gin Gly Gin Asn Ala Gly Ala Gly Cys Thr Phe Ala Ser Ser

20 25 30

He Ala Ser His Leu Val Lys Gly Asp Lys Leu Leu Pro Ala Val Glu

35 40 45

Ser Ser Lys Ala Phe Val Tyr Arg Ala He Ala Gin Ala Asp Gin Tyr

50 55 60

Gly Val Arg Gin Tyr Glu Ala Asn Lys Asn Asn 65 70 75

(2) INFORMATION FOR SEQ ID NO: 93:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 65 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 93:

Val He Ser Val Arg Glu Lys Ser Leu Lys Val Pro Ala He Leu Glu

1 5 10 15

Ala Val Glu Ala Thr Leu Gly Arg Pro Ala Phe Val Ser Phe Asp Ala

20 25 30

Glu Lys Leu Glu Gly Ser Leu Thr Arg Leu Pro Glu Arg Asp Glu He

35 40 45

Asn Pro Glu He Asn Glu Ala Leu Val Val Glu Phe Tyr Asn Lys Met 50 55 60

198

SUBSTTTUTE SHEET (RULE 26) Leu 65

(2) INFORMATION FOR SEQ ID NO: 94:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 134 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 94:

Val He Val Glu Lys Glu Glu Lys Gly Glu Glu Met Lys Pro Val He

1 5 10 15

Ser He He Met Gly Ser Lys Ser Asp Trp Ala Thr Met Gin Lys Thr

20 25 30

Ala Glu Val Leu Asp Arg Phe Gly Val Ala Tyr Glu Lys Lys Val Val

35 40 45

Ser Ala His Arg Thr Pro Asp Leu Met Phe Lys His Ala Glu Glu Ala

50 55 60

Arg Ser Arg Gly He Lys He He He Ala Gly Ala Gly Gly Ala Ala 65 70 75 80

His Leu Pro Gly Met Val Ala Ala Lys Thr Thr Leu Pro Val He Gly

85 90 95

Val Pro Val Lys Ser Arg Ala Leu Ser Gly Val Asp Ser Leu Tyr Ser

100 105 110

He Val Gin Met Pro Gly Gly Val Pro Val Ala Thr Met Ala He Gly

115 120 125

Glu Leu Phe Phe Arg He 130

(2) INFORMATION FOR SEQ ID NO: 95:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 66 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

( ii ) MOLECULE TYPE : None

199

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 95:

Val Arg Xaa Xaa Ala Pro Ser Thr Cys Xaa Trp Val Gly His Met Ala

1 5 10 15

Ser Gly Leu Arg His Asp Thr Lys Ala Pro Tyr Ser Asp Ser Xaa Xaa

20 25 30

Leu Gly Leu Arg Leu Phe Asn Leu Thr Thr Gin Gin Asn Xaa Thr Arg

35 40 45

Arg Phe He Leu Gin Lys Ala Xaa Ser His Pro Leu Thr Gly Ser Asn

50 55 60

Leu Leu 65

(2) INFORMATION FOR SEQ ID NO: 96:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 46 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 96:

Val Asp Asp Thr Asn Thr Leu Asn Val His He His Ala Leu Arg Gin

1 5 10 15

Glu Leu Ala Lys Tyr Ser Ser Asp Gin Thr Pro Thr He Lys Thr Val

20 25 30

Trp Gly Leu Gly Tyr Lys He Glu Lys Pro Arg Gly Gin Thr 35 40 45

(2) INFORMATION FOR SEQ ID NO: 97:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 169 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

200

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 97:

Val He Tyr Asn He Pro Gin Leu Ala Gly Val Ala Leu Thr Pro Ser

1 5 10 15

Leu Tyr Thr Glu Met Leu Lys Asn Pro Arg Val He Gly Val Lys Asn

20 25 30

Ser Ser Met Pro Val Gin Asp He Gin Thr Phe Val Ser Leu Gly Gly

35 40 45

Glu Asp His He Val Phe Asn Gly Pro Asp Glu Gin Phe Leu Gly Gly

50 55 60

Arg Leu Met Gly Ala Arg Ala Gly He Gly Gly Thr Tyr Gly Ala Met 65 70 75 80

Pro Glu Leu Phe Leu Lys Leu Asn Gin Leu He Ala Asp Lys Asp Leu

85 90 95

Glu Thr Ala Arg Glu Leu Gin Tyr Ala He Asn Ala He He Gly Lys

100 105 110

Leu Thr Ser Ala His Gly Asn Met Tyr Gly Val He Lys Glu Val Leu

115 120 125

Lys He Asn Glu Gly Leu Asn He Gly Ser Val Arg Ser Pro Leu Thr

130 135 140

Pro Val Thr Glu Glu Asp Arg Pro Val Val Glu Ala Ala Ala Ala Leu 145 150 155 160

He Arg Glu Thr Lys Glu Arg Phe Leu 165

(2) INFORMATION FOR SEQ ID NO: 98:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 288 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 98:

Val Thr Tyr Asp Thr He Gin Phe Lys Val Leu Lys Ala Val He Asp

1 5 10 15

Gin Ala Phe Leu Arg Val Lys Gly Tyr Thr Leu Asn Gly His Thr Leu

20 25 30

Pro Gly Gin Val Gin Gin Phe Asn Gin Val Phe He Asn Asn His Arg 35 40 45

201

SUBSTTTUTE SHEET (RULE 26) He Thr Pro Glu Val Thr Tyr Lys Lys He Asn Glu Thr Thr Ala Glu

50 55 60

Tyr Leu Met Lys Leu Arg Asp Asp Ala His Leu He Asn Ala Glu Met 65 70 75 80

Thr Val Arg Leu Gin Val Val Asp Asn Gin Leu His Phe Asp Val Thr

85 90 95

Lys He Val Asn His Asn Gin Val Thr Pro Gly Gin Lys He Asp Asp

100 105 110

Glu Arg Lys Leu Leu Ser Ser He Ser Phe Leu Gly Asn Ala Leu Val

115 120 125

Ser Val Ser Ser Asp Gin Thr Gly Ala Lys Phe Asp Gly Ala Thr Met

130 135 140

Ser Asn Asn Thr His Val Ser Gly Asp Asp His He Asp Val Thr Asn 145 150 155 160

Pro Met Lys Asp Leu Ala Lys Gly Tyr Met Tyr Gly Phe Val Ser Thr

165 170 175

Asp Lys Leu Ala Ala Gly Val Trp Ser Asn Ser Gin Asn Ser Tyr Gly

180 185 190

Gly Gly Ser Asn Asp Trp Thr Arg Leu Thr Ala Tyr Lys Glu Thr Val

195 200 205

Gly Asn Ala Asn Tyr Val Gly He His Ser Ser Glu Trp Gin Trp Glu

210 215 220

Lys Ala Tyr Lys Gly He Val Phe Pro Glu Tyr Thr Lys Glu Leu Pro 225 230 235 240

Ser Ala Lys Val Val He Thr Glu Asp Ala Asn Ala Asp Lys Lys Val

245 250 255

Asp Trp Gin Asp Gly Ala He Ala Tyr Arg Ser He Met Asn Asn Pro

260 265 270

Gin Gly Trp Glu Lys Val Lys Asp He Thr Ala Met Thr Leu Val Thr 275 280 285

(2) INFORMATION FOR SEQ ID NO: 99:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 66 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 99:

202

SUBSTTTUTE SHEET (RULE 26) Val He Leu Glu Gly Asn Tyr Arg Ala Thr Ala Gly Arg Glu Glu Met

1 5 10 15

Lys Glu Ala He Leu Glu Tyr Gin Ala Asn Pro Ala Ala Leu Lys Asp

20 25 30

Leu Lys Glu Lys Ala Lys Asn He Ser Arg Glu Tyr Ser Glu Glu His

35 40 45

Leu Leu Gin He Trp Leu Asp Phe Tyr Glu Lys Gin Ala Ala Leu Gly

50 55 60

Thr Lys 65

(2) INFORMATION FOR SEQ ID NO: 100:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 107 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 100:

Val Thr Phe Leu Asp Asp Tyr His Lys Lys His Asn Tyr Pro Leu Phe

1 5 10 15

Tyr Glu Ser Tyr Leu Gin Asn Val Met Glu Phe Leu Glu Ser Gin Asp

20 25 30

He Lys Asn Gly Val Asp Ala Phe Val Asp Asp His Gin Asn Leu Val

35 40 45

Phe Val Leu Tyr Gly Gin Gly Tyr Arg Ala Glu Gly Lys Glu Gly He

50 55 60

Leu Thr Thr Gin Val Thr Val Lys Ala Tyr Asp Glu Asp Lys Lys Pro 65 70 75 80

He Asn Phe Ala Asn Leu Leu Asp Ser Leu He Val Ser Glu Tyr Gin

85 90 95

Met Glu Pro Asn Leu Trp Glu Val Ser Tyr Asp 100 105

(2) INFORMATION FOR SEQ ID NO: 101:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 185 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 101:

Val Arg Lys Ser Val Pro Arg Pro Arg Leu Arg Gin Arg Ser Leu Ser

1 5 10 15

Lys Val Ala Arg Ser Arg Leu Lys He Lys Lys Leu Ser Lys Val Lys

20 25 30

His Glu Gly Gly Val Val He Glu Gly Ala Ser Gly Leu Leu Val Arg

35 40 45

He Ala Lys Cys Cys Asn Pro Val Pro Gly Asp Asp He Val Gly Tyr

50 55 60

He Thr Lys Gly Arg Gly Val Ala He His Arg Val Asp Cys Met Asn 65 70 75 80

Leu Arg Ala Gin Glu Asn Tyr Glu Gin Arg Leu Leu Asp Val Glu Trp

85 90 95

Glu Asp Gin Tyr Ser Ser Ser Asn Lys Glu Tyr Met Ala His He Asp

100 105 110

He Tyr Gly Leu Asn Arg Thr Gly Leu Leu Asn Asp Val Leu Gin Val

115 120 125

Leu Ser Asn Thr Thr Lys Asn He Ser Thr Val Asn Ala Gin Pro Thr

130 135 140

Lys Asp Met Lys Phe Ala Asn He His Val Ser Phe Gly He Ala Asn 145 150 155 160

Leu Ser Thr Leu Thr Thr Val Val Asp Lys He Lys Ser Val Pro Glu

165 170 175

Val Tyr Ser Val Lys Arg Thr Asn Gly 180 185

(2) INFORMATION FOR SEQ ID NO: 102:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 115 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 102:

204

SUBSTTTUTE SHEET (RULE 26) Val He Val Phe Leu Val Tyr Leu He He Thr Val Gin Lys Leu Gly

1 5 10 15

Arg Val He Asp Glu Thr Glu Lys Thr He Lys Thr Leu Thr Ser Asp

20 25 30

Val Asp Val Thr Leu His His Thr Asn Glu Leu Leu Ala Lys Val Asn

35 40 45

Val Leu Ala Asp Asp He Asn Val Lys Val Ala Thr He Asp Pro Leu

50 55 60

Phe Ser Ala Val Ala Asp Leu Ser Leu Ser Val Ser Asp Leu Asn Asp 65 70 75 80

His Ala Arg Val Leu Ser Lys Lys Ala Ser Ser Ala Gly Ser Lys Thr

85 90 95

Leu Lys Thr Gly Ala Ser Leu Ser Ala Leu Arg Leu Ala Ser Lys Phe

100 105 110

Phe Lys Lys 115

(2) INFORMATION FOR SEQ ID NO: 103:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 106 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 103:

Val Thr Gly Asn Trp Gin He Leu Phe Gin Gly Lys Met Thr Val Phe

1 5 10 15

Ser Trp Leu He Gly Pro Cys Ser Ser Asp Asn Glu Glu Ala Val Leu

20 25 30

Glu Tyr Ala Arg Arg Leu Ser Ala Leu Gin Lys Lys Val Ala Asp Lys

35 40 45

He Phe Met Val Met Arg Val Tyr Thr Ala Lys Pro Arg Thr Asn Gly

50 55 60

Asp Gly Tyr Lys Gly Leu Val His Gin Pro Asp Thr Ser Lys Ala Pro 65 70 75 80

Thr Leu He Asn Gly Leu Gin Ala Val Arg Gin Leu His Tyr Arg Val

85 90 95

Asp Tyr Arg Asp Trp Phe Asp Asn Gly Arg

205

SUBSTTTUTE SHEET (RULE 26) 100 105

(2) INFORMATION FOR SEQ ID NO: 104:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 71 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 104:

Val Gly Thr Gly He He Gly Ser He Val Ser Tyr Pro Val Met Val

1 5 10 15

Leu Phe Thr Gly Ser Ala Ala Lys Leu Ser Trp Phe He Tyr Thr Pro

20 25 30

Arg Phe Phe Gly Ala Thr Leu He Gly Thr Ala He Ser Phe He Ala

35 40 45

Phe Arg Phe Leu He Lys Gin Glu Phe Phe Lys Lys Val Gin Gly Tyr

50 55 60

Phe Phe Ala Glu Arg He Glu 65 70

(2) INFORMATION FOR SEQ ID NO: 105:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 98 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 105:

Val Ala He Ala Arg Gly Leu Ser Met Asn Pro Asp He Met Leu Phe

1 5 10 15

Asp Glu Pro Asn Ser Ala Leu Asp Pro Glu Met Val Gly Glu Val He

20 25 30

Asn Val Met Lys Glu Leu Ala Glu Gin Gly Met Thr Met He He Val 35 40 45

206

SUBSTTTUTE SHEET (RULE 26) Thr His Glu Met Gly Phe Ala Arg Gin Val Ala Asn Arg Val He Phe

50 55 60

Thr Ala Asp Gly Glu Phe Leu Glu Asp Gly Thr Pro Asp Gin He Phe 65 70 75 80

Asp Asn Pro Gin His Pro Arg Leu Lys Glu Phe Leu Asp Lys Val Leu

85 90 95

Asn Val

(2) INFORMATION FOR SEQ ID NO: 106:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 132 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 106:

Val Gin Ala Val Ser Glu Ser Ala Ala Ala Pro Val Arg Ala Lys Val

1 5 10 15

Arg Pro Thr Tyr Ser Thr Asn Ala Ser Ser Tyr Pro He Gly Glu Cys

20 25 30

Thr Trp Gly Val Lys Thr Leu Ala Pro Trp Ala Gly Asp Tyr Trp Gly

35 40 45

Asn Gly Ala Gin Trp Ala Thr Ser Ala Ala Ala Ala Gly Phe Arg Thr

50 55 60

Gly Ser Thr Pro Gin Val Gly Ala He Ala Cys Trp Asn Asp Gly Gly 65 70 75 80

Tyr Gly His Val Ala Val Val Thr Ala Val Glu Ser Thr Thr Arg He

85 90 95

Gin Val Ser Glu Ser Asn Tyr Ala Gly Asn Arg Thr He Gly Asn His

100 105 110

Arg Gly Trp Phe Asn Pro Thr Thr Thr Ser Glu Gly Phe Val Thr Tyr

115 120 125

He Tyr Ala Asp 130

(2) INFORMATION FOR SEQ ID NO: 107:

(i) SEQUENCE CHARACTERISTICS:

207

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 86 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 107:

Val He Leu Leu Asn Ser Glu Glu Lys Val Lys Lys Glu Arg Arg Ser

1 5 10 15

Lys Glu Arg He Ser Thr Thr Lys Lys Gly Phe Phe Arg Met Val Leu

20 25 30

Arg Tyr His Leu Thr Leu Leu Gly Gin Gly Thr Gly Val Val Thr Val

35 40 45

Leu Phe Thr Ser Ala Phe Leu Pro Tyr Leu Met Met He Gly Leu He

50 55 60

Ser Lys He Arg Asp Ser Gin He Val Pro Asp He His Pro Pro Tyr 65 70 75 80

Trp Leu Pro Phe Phe Leu 85

(2) INFORMATION FOR SEQ ID NO: 108:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 308 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 108:

Val Thr Pro Leu Ser Leu Leu Cys Leu Arg Lys Cys Val Arg Asp Glu

1 5 10 15

Asn Val Phe Leu Met Gly Glu Asp Val Gly Val Phe Gly Gly Asp Phe

20 25 30

Gly Thr Ser Val Gly Met Leu Glu Glu Phe Gly Pro Glu Arg Val Arg

35 40 45

Asp Cys Pro He Ser Glu Ala Ala He Ser Gly Ala Ala Ala Gly Ala

50 55 60

Ala Met Thr Gly Leu Arg Pro He Val Asp Met Thr Phe Met Asp Phe

208

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Ser Val He Ala Met Asp Asn He Val Asn Gin Ala Ala Lys Thr Arg

85 90 95

Tyr Met Phe Gly Gly Lys Gly Gin Val Pro Met Thr Val Arg Cys Ala

100 105 110

Ala Gly Asn Gly Val Gly Ser Ala Ala Gin His Ser Gin Ser Leu Glu

115 120 125

Ser Trp Phe Thr His He Pro Gly Leu Lys Val Val Ala Pro Gly Thr

130 135 140

Pro Ala Asp Met Lys Gly Leu Leu Lys Ser Ser He Arg Asp Asn Asn 145 150 155 160

Pro Val He He Leu Glu Tyr Lys Ser Glu Phe Asn Gin Lys Gly Glu

165 170 175

Val Pro Val Asp Pro Asp Tyr Thr He Pro Leu Gly Val Gly Glu He

180 185 190

Lys Arg Gin Gly Thr Asp Val Thr Val Val Thr Tyr Gly Lys Met Leu

195 200 205

Arg Arg Val Val Gin Ala Ala Glu Glu Leu Ala Glu Glu Gly He Ser

210 215 220

Val Glu He Val Asp Pro Arg Thr Leu Val Pro Leu Asp Lys Asp He 225 230 235 240

He He Asn Ser Val Lys Lys Thr Gly Lys Val Val Leu Val Asn Asp

245 250 255

Ala His Lys Thr Ser Gly Tyr He Gly Glu He Ser Ala He He Ser

260 265 270

Glu Ser Glu Ala Phe Asp Tyr Leu Asp Ala Pro He Arg Arg Cys Ala

275 280 285

Gly Glu Asp Val Pro Met Pro Tyr Ala Gin Asn Leu Lys Met Cys Asn

290 295 300

Asp Ser Asn Ser 305

(2) INFORMATION FOR SEQ ID NO: 109:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 191 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 109:

209

SUBSTTTUTΈ SHEET (RULE 26) Val Asp Gly Ala Thr Thr He Asp He Gly Ala Ser Thr Gly Gly Phe

1 5 10 15

Thr Asp Val Met Leu Gin Asn Ser Ala Lys Leu Val Phe Ala Val Asp

20 25 30

Val Gly Thr Asn Gin Leu Ala Trp Lys Leu Arg Gin Asp Pro Arg Val

35 40 45

Val Ser Met Glu Gin Phe Asn Phe Arg Tyr Ala Glu Lys Thr Asp Phe

50 55 60

Glu Gin Glu Pro Ser Phe Ala Ser He Asp Val Ser Phe He Ser Leu 65 70 75 80

Ser Leu He Leu Pro Ala Leu His Arg Val Leu Ala Asp Gin Gly Gin

85 90 95

Val Val Ala Leu Val Lys Pro Gin Phe Glu Ala Gly Arg Glu Gin He

100 105 110

Gly Lys Asn Gly He He Arg Asp Ala Lys He His Gin Asn Val Leu

115 120 125

Glu Ser Val Thr Ala Met Ala Val Glu Ala Gly Phe Ser Val Leu Gly

130 135 140

Leu Asp Phe Ser Pro He Gin Gly Gly His Gly Asn He Glu Phe Leu 145 150 155 160

Val Tyr Leu Lys Lys Glu Lys Ser Ala Ser Asn Gin He Leu Ala Glu

165 170 175

He Lys Glu Ala Val Glu Arg Ala His Ser Gin Phe Lys Asn Glu 180 185 190

(2) INFORMATION FOR SEQ ID NO: 110:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 54 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 110:

Val Ser Ser Asp Val Lys Trp Leu Cys Gin Asn His Pro Lys Trp His

1 5 10 15

Lys Leu Arg Gly He Gly Met Thr Arg Asn Thr He Asp Arg Asp Gly

20 25 30

He Thr Ser Gin Asp Val Arg Tyr Phe He Phe Asn Phe Lys Leu Asp

210

SUBSTTTUTE SHEET (RULE 26) 35 40 45

Val Asp Asp Leu Leu Pro 50

(2) INFORMATION FOR SEQ ID NO : 111:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 126 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 111:

Val Asp Leu Gin Ser Lys Asn Trp Ser Phe Val His Arg Phe Ser Glu

1 5 10 15

Glu Leu He Asp Gin His Tyr Gin Asp Leu Val Gly Gin Ser Phe Tyr

20 25 30

Pro Pro He Arg Glu Phe Met Thr Ser Gly Pro Val Leu Val Gly Val

35 40 45

He Ser Gly Pro Lys Val He Glu Thr Trp Arg Thr Met Met Gly Ala

50 55 60

Thr Arg Pro Glu Glu Ala Leu Pro Gly Thr He Arg Gly Asp Phe Ala 65 70 75 80

Lys Ala Ala Gly Glu Asn Glu He He Gin Asn Val Val His Gly Ser

85 90 95

Asp Ser Glu Lys Ser Gin Leu Ser Arg Glu He Ala Pro Leu Val Leu

100 105 110

Arg Val Asp Trp Leu Asn Gin Leu Val Lys Ser Ser Phe Glu 115 120 125

(2) INFORMATION FOR SEQ ID NO: 112:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 50 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

211

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 112:

Val Leu Lys Gly Val Leu Thr Leu Arg Glu Leu Thr Asn Asp Arg Asp

1 5 10 15

Ala Asp He Asn Asp Phe Val Lys Val Gly Glu Val Leu Asp Val Leu

20 25 30

Val Leu Arg Gin Val Val Gly Lys Asp Thr Asp Thr Val Thr Tyr Leu

35 40 45

Val He 50

(2) INFORMATION FOR SEQ ID NO: 113:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 52 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 113:

Val Gly Glu Pro Phe Ala Asn Leu Ser Asp Leu Leu Asp Thr Tyr Tyr

1 5 10 15

Lys Asp Lys Ala Glu Arg Asp Arg Val Lys Gin Gin Ala Ser Glu Leu

20 25 30

He Arg Arg Val Glu Asn Glu Leu Gin Lys Asn Arg His Lys Leu Lys

35 40 45

Lys Gin Glu Lys 50

(2) INFORMATION FOR SEQ ID NO: 114:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 113 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 114:

212

SUBSTTTUTE SHEET (RULE 26) Val Lys Asp Lys Thr Leu He He Gin His Ser Gly Ala Tyr He Ala

1 5 10 15

Arg Tyr Ser He Thr Trp Glu Glu Val Pro Val Asp Lys Asp Gly Asn

20 25 30

Gin Val Val Arg Ser His Ser Trp Glu Gly Asn Gly Arg Asn Gin Thr

35 40 45

Ala Gly Phe Val Leu Asn Leu Pro He Lys Glu Asn Met Arg Asn Leu

50 55 60

Arg Val Lys He Glu Lys Lys Thr Gly Leu Leu Trp Asn Arg Trp Gin 65 70 75 80

Thr He Tyr Glu Asn Arg Pro He Leu Ala Gin Pro His Arg Lys He

85 90 95

Thr His Trp Gly Thr Thr Leu Asn Ser Lys Val Ser Asp Asp Asp Val

100 105 110

Leu

(2) INFORMATION FOR SEQ ID NO: 115:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 40 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 115:

Val Leu Gly Ala Gly Lys Arg Leu Thr Gly Tyr Ala Ala Gly Val Glu

1 . 5 - 10 15

Lys Lys Ala Trp Leu Leu Glu His Glu Gly Val Asp Phe Lys Asp Arg

20 25 30

Asn Asn Arg Arg Arg Ser Thr Cys 35 40

(2) INFORMATION FOR SEQ ID NO: 116:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 69 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

213

SUBSTTTUTE SHEET (RULE 26) (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 116:

Val His Val Cys Cys Ala Pro Cys Ser Thr Tyr Thr Leu Glu Tyr Leu

1 5 10 15

Thr Lys Tyr Ala Asp Val Thr He Tyr Phe Ala Asn Ser Asn He His

20 25 30

Pro Lys Ala Glu Tyr His Lys Arg Val Tyr Val Thr Lys Lys Phe Val

35 40 45

Ser Asp Phe Asn Glu Gin Thr Gly Asn Thr Val Gin Tyr Leu Glu Ala

50 55 60

Pro Tyr Glu Pro Asn 65

(2) INFORMATION FOR SEQ ID NO: 117:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 83 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 117:

Val Ala Met Asp Leu Gly Phe Asp Tyr Phe Gly Ser Ala Leu Thr He

1 5 10 15

Ser Pro His Lys Asn Ser Gin Thr He Asn Ser He Gly He Asp Val

20 25 30

Gin Lys He Tyr Thr Pro His Tyr Leu Pro Asn Asp Phe Lys Lys Asn

35 40 45

Gin Gly Tyr Lys Arg Ser Val Glu Met Arg Glu Glu Tyr Asp He Tyr

50 55 60

Arg Gin Cys Tyr Cys Gly Cys Val Tyr Ala Ala Gin Ala Gin Asn He 65 70 75 80

Asp Leu Val

(2) INFORMATION FOR SEQ ID NO: 118:

214

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 52 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 118:

Val Thr Asp Gly Val He Gin Val Asp Val Leu Gly Ser He Val Arg

1 5 10 15

Ser Glu Glu Trp Leu Leu Asp Asn Leu Ser Lys Gin Gly His Asp Asn

20 25 30

Val Ala Asn He Phe He Ala Glu Tyr Asp Lys Gly Ala Val Thr Val

35 40 45

Val Thr Tyr Lys 50

(2) INFORMATION FOR SEQ ID NO: 119:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 206 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 119:

Val Arg Glu Tyr Arg Thr Tyr Glu Glu He Ala Ala Asp Phe Gly He

1 5 10 15

His Glu Ser Asn Leu He Arg Arg Ser Gin Trp Val Glu Val Thr Leu

20 25 30

Val Gin Ser Gly Val Thr He Ser Lys Thr His Leu Ser Ala Glu Asn

35 40 45

Thr Val He Val Asp Ala Thr Glu Val Lys He Asn Arg Pro Lys Lys

50 55 60

Gin Leu Ala Asn Asp Ser Gly Lys Lys Lys Phe His Ala Met Lys Ala 65 70 75 80

Gin Ala He Val Thr Ser Gin Gly Arg He Val Ser Leu Asp He Ala

215

SUBSTTTUTE SHEET (RULE 26) 85 90 95

Val Asn Tyr Cys His Asp Met Lys Leu Phe Lys Met Ser Arg Arg Asn

100 105 110

He Gly Gin Ala Gly Lys He Leu Ala Asp Ser Gly Tyr Gin Gly Pro

115 120 125

Met Lys He Tyr Pro Gin Ala Gin Thr Pro Arg Lys Ser Ser Lys Leu

130 135 140

Lys Pro Leu He Ala Glu Asp Lys Ala Tyr Asn His Ala Leu Ser Lys 145 150 155 160

Glu Arg Ser Lys Val Glu Asn He Phe Ala Lys Val Lys Thr Phe Lys

165 170 175

Met Phe Ser Thr Thr Tyr Arg Asn His Arg Lys Arg Phe Gly Leu Arg

180 185 190

Met Asn Leu He Ala Gly He He Asn Tyr Glu Leu Gly Phe 195 200 205

(2) INFORMATION FOR SEQ ID NO: 120:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 91 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 120:

Val Met Gly Pro Gin Gly Asn Gly Phe Asp Leu Ser Asp Leu Asp Glu

1 5 10 15

Gin Asn Gin Val Leu Leu Val Gly Gly Gly He Gly Val Pro Pro Leu

20 25 30

Leu Glu Val Ala Lys Glu Leu His Glu Arg Gly Val Lys Val Val Thr

35 40 45

Val Leu Gly Phe Ala Asn Lys Asp Ala Val He Leu Lys Thr Glu Leu

50 55 60

Ala Gin Tyr Gly Gin Val Phe Val Thr Thr Asp Asp Gly Ser Tyr Gly 65 70 75 80

He Lys Gly Asn Val Pro Leu Leu Ser Met He 85 90

(2) INFORMATION FOR SEQ ID -NO: 121:

216

SUBSTTTUTΕ SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 222 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 121:

Val Lys Met Val Leu Phe Ser Ala Gin Glu Gin Leu Tyr Tyr Lys Glu

1 5 10 15

Lys He Met Thr Thr Asn Arg Leu Gin Val Ser Leu Pro Gly Leu Asp

20 25 30

Leu Lys Asn Pro He He Pro Ala Ser Gly Cys Phe Gly Phe Gly Gin

35 40 45

Glu Tyr Ala Lys Tyr Tyr Asp Leu Asp Leu Leu Gly Ser He Met He

50 55 60

Lys Ala Thr Thr Leu Glu Pro Arg Phe Gly Asn Pro Thr Pro Arg Val 65 70 75 80

Ala Glu Thr Pro Ala Gly Met Leu Asn Ala He Gly Leu Gin Asn Pro

85 90 95

Gly Leu Glu Val Val Leu Ala Glu Lys Leu Pro Trp Leu Glu Arg Glu

100 105 110

Tyr Pro Asn Leu Pro He He Ala Asn Val Ala Gly Phe Ser Lys Gin

115 120 125

Glu Tyr Ala Ala Val Ser His Gly He Ser Lys Ala Thr Asn He Lys

130 135 140

Ala He Glu Leu Asn He Ser Cys Pro Asn Val Asp His Cys Asn His 145 150 155 160

Gly Leu Leu He Gly Gin Asp Pro Asp Leu Ala Tyr Asp Val Val Lys

165 170 175

Ala Ala Val Glu Ala Ser Glu Val Pro Val Tyr Val Lys Leu Thr Pro

180 185 190

Ser Val Thr Asp He Val Thr Val Ala Lys Ala Ala Glu Asp Ala Gly

195 200 205

Ala Ser Gly Leu Thr Met He He Leu Trp Trp Asp Ala Leu 210 215 220

(2) INFORMATION FOR SEQ ID NO: 122:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 155 amino acids

217

SUBSTTTUTE SHEET (RULE 26) (B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 122:

Val Ala Thr Gly Gin Asp Lys Ala His Ser He Leu Ala Ser Asn Glu

1 5 10 15

Gly Thr Leu His Tyr Leu Val Pro Leu Lys Gin Gly Met Ser He Gin

20 25 30

Gin Gly Gin Thr He Ala Glu Val Ser Gly Lys Glu Lys Gly Tyr Tyr

35 40 45

Val Glu Ala Phe Val Leu Ala Ser Asp He Ser Arg Val Ser Lys Gly

50 55 60

Ala Lys Val Asp Val Ala He Thr Gly Val Asn Ser Gin Lys Tyr Gly 65 70 75 80

Thr Leu Lys Gly Gin Val Arg Gin He Asp Ser Gly Thr He Ser Gin

85 90 95

Glu Thr Lys Glu Gly Asn He Ser Leu Tyr Lys Val Met He Glu Leu

100 105 110

Glu Thr Leu Thr Leu Lys His Gly Ser Glu Thr Val He Leu Gin Lys

115 120 125

Asp Met Pro Val Glu Val Arg He Val Tyr Asp Lys Glu Thr Tyr Leu

130 135 140

Asp Trp He Leu Glu Met Leu Ser Phe Lys Gin 145 150 155

(2) INFORMATION FOR SEQ ID NO: 123:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 219 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

( ii ) MOLECULE TYPE : None

(xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 123 :

Val Arg Val Pro Glu Thr He Thr Gin Glu Glu Leu Leu Asp Leu He 1 5 10 15

218

SUBSTTTUTE SHEET (RULE 26) Ala Lys Tyr Asn Gin Asp Pro Ala Trp His Gly He Leu Val Gin Leu

20 25 30

Pro Leu Pro Lys His He Asp Glu Glu Ala Val Leu Leu Ala He Asp

35 40 45

Pro Glu Lys Asp Val Asp Gly Phe His Pro Leu Asn Met Gly Arg Leu

50 55 60

Trp Ser Gly His Pro Val Met He Pro Ser Thr Pro Ala Gly He Met 65 70 75 80

Glu Met Phe His Glu Tyr Gly He Asp Leu Glu Gly Lys Asn Ala Val

85 90 95

Val He Gly Arg Ser Asn He Val Gly Lys Pro Met Ala Gin Leu Leu

100 105 110

Leu Ala Lys Asn Ala Thr Val Thr Leu Ala His Ser Arg Thr His Asn

115 120 125

Leu Ala Lys Val Ala Ala Lys Ala Asp He Leu Val Val Ala He Gly

130 135 140

Arg Ala Lys Phe Val Thr Ala Asp Phe Val Lys Pro Gly Ala Val Val 145 150 155 160

He Asp Val Gly Met Asn Arg Asp Glu Asn Gly Lys Leu Cys Gly Asp

165 170 175

Val Asp Tyr Glu Ala Val Ala Pro Leu Ala Ser His He Thr Pro Val

180 185 190

Pro Gly Gly Val Gly Pro Met Thr He Thr Met Leu Met Glu Gin Thr

195 200 205

Tyr Gin Ala Ala Leu Arg Thr Leu Asp Arg Lys 210 215

(2) INFORMATION FOR SEQ ID NO: 124:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 83 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 124:

Val Gly Val Tyr Leu Ser Glu Gly Leu Pro Asp Leu He Arg Val Thr

1 5 10 15

Thr Val Thr Leu He Ser Leu Val Gly Glu Thr Ala Met Ala Gly Ala 20 25 30

219

SUBSTTTUTE SHEET (RULE 26) Val Gly Ala Gly Gly He Gly Asn Val Ala He Ala Tyr Gly Phe Asn

35 40 45

Arg Tyr Asn His Asp Val Thr He Leu Ala Thr He Val He He Leu

50 55 60

He He Phe Ala He Gin Phe Leu Gly Asp Phe Leu Thr Lys Lys Leu 65 70 75 80

Ser His Lys

(2) INFORMATION FOR SEQ ID NO: 125:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 223 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 125:

Val Leu Pro Leu Tyr Leu Leu Phe Val Pro Tyr Gly Lys Ser Lys Lys

1 5 10 15

Glu Val Lys Lys Arg Ala Lys Glu Ala Ser Arg Leu Thr Arg Glu Met

20 25 30

Lys Gly Leu He Phe Thr Leu Ala He Glu Ala Ala Val Val Val Cys

35 40 45

Thr Asn Thr Ala He Thr He Arg He Pro Ser Leu Met Val Glu Arg

50 55 60

Gly Leu Gly Asp Ala Gin Leu Ser Ser Phe Val Leu Ser He Met Gin 65 70 75 80

Leu He Gly He Val Ala Gly Val Ser Phe Ser Phe Leu He Ser He

85 90 95

Phe Lys Glu Lys Leu Leu Leu Trp Ser Gly He Thr Phe Gly Leu Gly

100 105 110

Gin He Val He Ala Leu Ser Ser Ser Leu Trp Val Val Val Ala Gly

115 120 125

Ser Val Leu Ala Gly Phe Ala Tyr Ser Val Val Leu Thr Thr Val Phe

130 135 140

Gin Leu Val Ser Glu Arg He Pro Ala Lys Leu Leu Asn Gin Ala Thr 145 150 155 160

Ser Phe Ala Val Leu Gly Cys Ser Phe -Gly Ala Phe Thr Thr Pro Phe 165 170 175

220

SUBSTTTUTΕ SHEET (RULE 26) Val Leu Gly Ala He Gly Leu Leu Thr His Asn Gly Met Leu Val Phe

180 185 190

Ser He Leu Gly Gly Trp Leu He Val He Ser He Phe Val Met Tyr

195 200 205

Leu Leu Gin Lys Arg Ala Leu Gly Leu He Pro Lys Phe Phe Phe 210 215 220

(2) INFORMATION FOR SEQ ID NO: 126:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 119 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 126:

Val Val Ala Gly Pro Glu Gly Leu Asp Glu Ala Gly Leu Asn Gly Thr

1 5 10 15

Thr Xaa He Ala Leu Xaa Glu Asn Gly Glu He Ser Leu Ser Ser Phe

20 25 30

Thr Pro Glu Asp Leu Gly Met Glu Gly Tyr Ala Met Glu Asp He Arg

35 40 45

Gly Gly Asn Ala Gin Glu Asn Ala Glu He Leu Leu Ser Val Leu Lys

50 55 60

Asn Glu Ala Ser Pro Phe Leu Glu Thr Thr Val Leu Asn Ala Gly Leu 65 70 75 80

Gly Phe Tyr Ala Asn Gly Lys He Asp Ser He Lys Glu Gly Val Ala

85 90 95

Leu Ala Arg Gin Val He Ala Arg Gly Lys Ala Leu Glu Lys Leu Arg

100 105 110

Leu Leu Gin Glu Tyr Gin Lys 115

(2) INFORMATION FOR SEQ ID NO: 127:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 112 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

221

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 127:

Val Asp He Val Gin Gin Ala Gin Thr Tyr Glu Glu Asn Gly Ala Val

1 5 10 15

Met He Ser Val Leu Thr Asp Glu Val Phe Phe Lys Gly His Leu Asp

20 25 30

Tyr Leu Arg Glu He Ser Ser Gin Val Glu He Pro Thr Leu Asn Lys

35 40 45

Asp Phe He He Asp Glu Lys Gin He He Arg Ala Arg Asn Ala Gly

50 55 60

Ala Thr Val He Leu Leu He Val Ala Ala Leu Ser Glu Glu Arg Leu 65 70 75 80

Lys Glu Leu Tyr Asp Tyr Ala Thr Glu Leu Gly Leu Glu Val Leu Val

85 90 95

Glu Thr His Asn Leu Ala Glu Leu Glu Val Ala His Arg Leu Gly Gly 100 105 110

(2) INFORMATION FOR SEQ ID NO: 128:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 65 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 128:

Val Ser Glu Lys His Ala Gly Phe Met He Asn Val Ala Asp Gly Thr

1 5 10 15

Ala Lys Asp Tyr Glu Asp Leu He Gin Ser Val He Glu Lys Val Lys

20 25 30

Glu His Ser Gly He Thr Leu Glu Arg Glu Val Arg He Leu Gly Glu

35 40 45

Ser Leu Ser Val Ala Lys Met Tyr Ala Gly Gly Phe Thr Pro Cys Lys

50 55 60

Arg 65

222

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 129:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 29 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 129:

Val Glu Arg He He Arg Lys Ala Phe Ala He Glu Leu Gin Glu He

1 5 10 15

Ala Glu Lys Ser Leu Leu Val Ser He Ser Lys Met Phe 20 25

(2) INFORMATION FOR SEQ ID NO: 130:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 88 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 130:

Val Arg He Gly Asn Thr Val Leu Ala Asn Val Thr Ser Gly Val Ala

1 5 10 15

Lys Gin Ala Ser Lys Ala Ala Gin Ala Ser Asn Leu Gly Gly Gly Ala

20 25 30 Glu Val Asp Gly Phe Ser Lys Thr Leu Ser Ser Leu Asp He Ser He

35 40 45

Gin Thr Ser Asp Phe He He He Phe Val Leu Ala Leu Val Leu Val

50 55 60

Val Leu Val Met Ala Leu Ala Ser Ser Asn Leu Leu Arg Lys Gin Pro 65 70 75 80

Lys Glu Leu Leu Leu Asp Gly Glu 85

(2) INFORMATION FOR SEQ ID NO: 131:

223

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 164 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 131:

Val Ser Asn Lys Thr Phe Pro He Leu Val Asn Lys Asp Pro Lys Thr

1 5 10 15

Gly Thr Tyr Ser Gly He Glu Thr Asp Leu Ala Lys Met Val Ala Asp

20 25 30

Glu Leu Lys Val Lys He His Tyr Val Pro Val Thr Ala Gin Thr Arg

35 40 45

Gly Pro Leu Leu Asp Asn Glu Gin Val Asp Met Asp He Ala Thr Phe

50 55 60

Thr He Thr Asp Glu Arg Lys Lys Leu Tyr Asn Phe Thr Ser Pro Tyr 65 70 75 80

Tyr Thr Asp Ala Ser Gly Phe Leu Val Asn Lys Ser Ala Lys He Lys

85 90 95

Lys He Glu Asp Leu Asn Gly Lys Thr He Gly Val Ala Gin Gly Ser

100 105 110

He Thr Gin Arg Leu He Thr Glu Leu Gly Lys Lys Lys Gly Leu Lys

115 120 125

Phe Lys Phe Val Glu Leu Gly Ser Tyr Pro Glu Leu He Thr Ser Leu

130 135 140

His Ala His Arg He Asp Ala Phe Ser Val Asp Arg Ser He Leu Ser 145 150 155 160

Gly Tyr Thr Ser

(2) INFORMATION FOR SEQ ID NO: 132:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 62 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

224

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 132:

Val Leu Glu Glu Leu Arg He Pro Ala Pro Asn Glu Phe Glu Asp Leu

1 5 10 15

Asp Leu Ser Pro Leu Asp Phe Lys Pro His He Ala Pro His Lys Phe

20 25 30

Glu Gly Met Val Glu Thr Ala Arg Asp Leu He Arg Asn Gly Asp Met

35 40 45

Phe Arg Cys Val Thr Gin Pro Ala Phe Ser Ser Arg Arg Ser 50 55 60

(2) INFORMATION FOR SEQ ID NO: 133:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 65 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 133:

Val Ser Ser Ser Phe Phe Thr Pro Leu Lys Gin Leu Ser Lys Phe Leu

1 5 10 15

He He Met Ala Met Ser Ala He Gly Leu Lys Thr Asn Leu Val Ala

20 25 30

Met Val Lys Ser Ser Gly Lys Ser He Val Leu Gly Ala Val Cys Trp

35 40 45

He Ala He He Leu Thr Ser Leu Gly Met Gin Thr Leu He Gly He

50 55 60

Phe 65

(2) INFORMATION FOR SEQ ID NO: 134:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 71 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

225

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 134:

Val Pro Glu Asp Tyr Arg He He Thr Ser Asp Asp Ser Gin He Ser

1 5 10 15

Arg Phe Thr Arg Pro Asn Leu Thr Thr He Ala Gin Pro Leu Tyr Asp

20 25 30

Leu Gly Ala He Ser Met Arg Met Leu Thr Lys He Met His Lys Glu

35 40 45

Glu Leu Glu Glu Arg Glu Val Leu Leu Pro His Gly Leu Thr Glu Arg

50 55 60

Ser Ser Thr Arg Lys Arg Lys 65 70

(2) INFORMATION FOR SEQ ID NO: 135:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 163 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 135:

Val Gly Gin Ser Gin Phe Leu Phe Lys Val Ser Tyr Ala Asp Gly Gin

1 5 10 15

Lys Ala Tyr Arg Val Asp Leu Pro Asp Leu Leu Thr Lys Thr Asp Trp

20 25 30

Gin He He Lys Ser Phe Leu Asp Val Leu Leu Ala Tyr Thr Gly Thr

35 40 45

Asp He Glu Gly Leu Asp Gly Phe Asp Phe Glu Ala Tyr Phe Gin Ala

50 55 60

Ser He Gin Ala Tyr Leu Ala Asp Pro Val Ala Arg Phe Thr He Cys 65 70 75 80

Gin Arg He Phe Asn Pro He Phe Phe Ser Arg Glu Asn Leu Lys Ser

85 90 95

Phe Leu Glu Ala Asp Gly Leu Ala Gin Phe Glu Ala Arg Val Arg Ala

100 105 110

Val Gin Glu Thr Asp Ala Tyr Phe Ala Arg Val Ser Phe Tyr Gin Asp 115 120 125

226

SUBSTTTUTE SHEET (RULE 26) Gly Glu Gly Lys Val His Gly Val Tyr His Leu Ala Gin Gly Val Lys

130 135 140

Thr Val Leu Pro Arg Glu Pro Phe Val Pro Ala Ala Tyr He Glu Arg 145 150 155 160

He Gly Gly

(2) INFORMATION FOR SEQ ID NO: 136:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 69 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 136:

Val Asp Lys Glu Val Gin Trp Glu He Asp Leu Val Gin He Thr Gly

1 5 10 15

Asp Gly Ser Lys Pro Glu Asp Tyr Glu Ser He Ala Arg Leu Asp Tyr

20 25 30

Ala Lys Phe Leu Glu Val Leu Pro Pro Ser Phe Tyr His Gin Leu Asp

35 40 45

Ala Asn Gin He Glu He Gin Pro He Leu Gly Gin Asp Phe Lys Thr

50 55 60

Leu Ala Gin Glu Lys 65

(2) INFORMATION FOR SEQ ID NO: 137:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 299 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 137:

Val He Leu Lys He Glu Asp Leu Val Met Ser He He Ser Thr Asp

227

SUBSTTTUTE SHEET (RULE 26) 1 5 10 15

Leu Thr Pro Phe Gin He Asp Asp Thr Leu Lys Ala Ala Leu Arg Glu

20 25 30

Asp Val His Ser Glu Asp Tyr Ser Thr Asn Ala He Phe Asp His His

35 40 45

Gly Gin Ala Lys Val Ser Leu Phe Ala Lys Glu Ala Gly Val Leu Ala

50 55 60

Gly Leu Thr Val Phe Gin Arg Val Phe Thr Leu Phe Asp Ala Glu Val 65 70 75 80

Thr Phe Gin Asn Pro His Gin Phe Lys Asp Gly Asp Arg Leu Thr Ser

85 90 95

Gly Asp Leu Val Leu Glu He He Gly Ser Val Arg Ser Leu Leu Thr

100 105 110

Cys Glu Arg Val Ala Leu Asn Phe Leu Gin His Leu Ser Gly He Ala

115 120 125

Ser Met Thr Ala Ala Tyr Val Glu Ala Leu Gly Asp Asp Cys He Lys

130 135 140

Val Phe Asp Thr Arg Lys Thr Thr Pro Asn Leu Arg Leu Phe Glu Lys 145 150 155 160

Tyr Ala Val Arg Val Gly Gly Gly Tyr Asn His Arg Phe Asn Leu Ser

165 170 175

Asp Ala He Leu Leu Lys Asp Asn His He Ala Ala Val Gly Ser Val

180 185 190

Gin Arg Ala He Ala Gin Ala Arg Ala Tyr Ala Pro Phe Val Lys Met

195 200 205

Val Glu Val Glu Val Glu Ser Leu Ala Ala Ala Glu Glu Ala Ala Ala

210 215 220

Ala Gly Ala Asp He He Met Leu Asp Asn Met Ser Leu Glu Gin He 225 230 235 240

Glu Gin Ala He Thr Leu He Ala Gly Arg Ser Arg He Glu Cys Ser

245 250 255

Gly Asn He Asp Met Thr Thr He Ser Arg Phe Arg Gly Leu Ala He

260 265 270

Asp Tyr Val Ser Ser Gly Ser Leu Thr His Ser Ala Lys Ser Leu Asp

275 280 285

Phe Ser Met Lys Gly Leu Thr Tyr Leu Asp Val 290 295

(2) INFORMATION FOR SEQ ID NO: 138:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 242 amino acids -

(B) TYPE: amino acid

228

SUBSTTTUTE SHEET (RULE 26) (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 138:

Val Glu Val Glu Val Pro Thr Gin Val Pro Ala His He Gly He He

1 5 10 15

Met Asp Gly Asn Gly Arg Trp Ala Lys Lys Arg Met Gin Pro Arg Val

20 25 30

Phe Gly His Lys Ala Gly Met Glu Ala Leu Gin Thr Val Thr Lys Ala

35 40 45

Ala Asn Lys Leu Gly Val Lys Val He Thr Val Tyr Ala Phe Ser Thr

50 55 60

Glu Asn Trp Thr Arg Pro Asp Gin Glu Val Lys Phe He Met Asn Leu 65 70 75 80

Pro Val Glu Phe Tyr Asp Asn Tyr Val Pro Glu Leu His Ala Asn Asn

85 90 95

Val Lys He Gin Met He Gly Glu Thr Asp Arg Leu Pro Lys Gin Thr

100 105 110

Phe Glu Ala Leu Thr Lys Ala Glu Glu Leu Thr Lys Asn Asn Thr Gly

115 120 125

Leu He Leu Asn Phe Ala Leu Asn Tyr Gly Gly Arg Ala Glu He Thr

130 135 140

Gin Ala Leu Lys Leu He Ser Gin Asp Val Leu Asp Ala Lys He Asn 145 150 155 160

Pro Gly Asp He Thr Glu Glu Leu He Gly Asn Tyr Leu Phe Thr Gin

165 170 175

His Leu Pro Lys Asp Leu Arg Asp Pro Asp Leu He He Arg Thr Ser

180 185 190

Gly Glu Leu Arg Leu Ser Asn Phe Leu Pro Trp Gin Gly Ala Tyr Ser

195 200 205

Glu Leu Tyr Phe Thr Asp Thr Leu Trp Pro Asp Phe Asp Glu Ala Ala

210 215 220

Leu Gin Glu Ala He Leu Ala Tyr Asn Arg Arg His Arg Arg Phe Gly 225 230 235 240

Gly Val

(2) INFORMATION FOR SEQ ID NO: 139:

(i) SEQUENCE CHARACTERISTICS:

229

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 183 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 139:

Val Val Ala Tyr Ser Val Leu He Ser He Met Leu Gly Thr Thr Val

1 5 10 15

Phe Ser Lys Ser Tyr Thr He Glu Asp Ala Val Phe Pro Leu Ala Met

20 25 30

Ser Phe Tyr Val Gly Phe Gly Phe Asn Ala Leu Leu Asp Ala Arg Val

35 40 45

Ala Gly Leu Asp Lys Ala Leu Leu Ala Leu Cys He Val Trp Ala Thr

50 55 60

Asp Ser Gly Ala Tyr Leu Val Gly Met Asn Tyr Gly Lys Arg Lys Leu 65 70 75 80

Ala Pro Arg Val Ser Pro Asn Lys Thr Leu Glu Gly Ala Leu Gly Gly

85 90 95

He Leu Gly Ala He Leu Val Thr He He Phe Met He Val Asp Ser

100 105 110

Thr Val Ala Leu Pro Tyr Gly He Tyr Lys Met Ser Val Phe Ala He

115 120 125

Phe Phe Ser He Ala Gly Gin Phe Gly Asp Leu Leu Glu Ser Ser He

130 135 140

Lys Arg His Phe Gly Val Lys Asp Ser Gly Lys Phe He Pro Gly His 145 150 155 160

Gly Gly Val Leu Asp Arg Phe Asp Ser Met Leu Leu Val Phe Pro He

165 170 175

Met His Leu Phe Gly Leu Phe 180

(2) INFORMATION FOR SEQ ID NO: 140:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 95 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

( i i ) MOLECULE TYPE : None

230

SUBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 140:

Val Asp Leu Leu Leu Ser Leu Arg Gin Val Val Met Leu Leu Lys Met

1 5 10 15

Glu Leu Arg He Phe Leu Tyr Phe Leu Ala Met He Ser He Asn He

20 25 30

Gly He Phe Asn Leu He Pro He Pro Ala Leu Asp Gly Gly Lys He

35 40 45

Val Leu Asn He Leu Glu Ala He Arg Arg Lys Pro Leu Lys Gin Glu

50 55 60

He Glu Thr Tyr Val Thr Leu Ala Gly Val Val He Met Val Val Leu 65 70 75 80

Met He Ala Val Thr Trp Asn Asp He Met Arg Leu Phe Phe Arg 85 90 95

(2) INFORMATION FOR SEQ ID NO: 141:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 183 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 141:

Val Glu Leu Met Ser Thr Val Gin Lys Ser Thr Phe Met Lys Cys Val

1 5 10 15

Asn Thr Leu Glu Trp Phe Phe Asn Ala Pro He His Leu Leu Asn Arg

20 25 30

He Tyr Arg Asn He Thr Phe Ala His Glu Arg Ala Gly Val Lys Asp

35 40 45

Lys Gin Val Leu Asp Glu He Val Glu Thr Ser Leu Ser Gin Ala Ala

50 55 60

Leu Trp Asp Gin Val Lys Asp Asp Leu His Lys Ser Ala Leu Thr Leu 65 70 75 80

Ser Gly Gly Gin Gin Gin Arg Leu Cys He Ala Arg Ala He Ser Val

85 90 95

Lys Pro Asp He Leu Leu Met Asp Glu Pro Ala Ser Ala Leu Asp Pro

100 105 110

He Ala Thr Met Gin Leu Glu Glu Thr Met Phe Glu Leu Lys Lys Asn

231

SUBSTTTUTE SHEET (RULE 26) 115 120 125

Phe Thr He He He Val Thr His Asn Met Gin Gin Ala Ala Arg Ala

130 135 140

Ser Asp Tyr Thr Gly Phe Phe Tyr Leu Gly Asp Leu He Glu Tyr Asp 145 150 155 160

Lys Thr Ala Thr He Phe Gin Asn Ala Lys Leu Gin Ser Thr Asn Asp

165 170 175

Tyr Val Ser Gly His Phe Gly 180

(2) INFORMATION FOR SEQ ID NO: 142:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 228 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 142:

Val Pro Lys Glu Ser Leu Thr Gin Val Leu Pro Arg Asp Leu His Ala

1 5 10 15

Glu Tyr Phe Ala Val Leu Ala Ser He Ala Thr Ser He Glu Arg Met

20 25 30

Ala Thr Glu He Arg Gly Leu Gin Lys Ser Glu Gin Arg Glu Val Glu

35 40 45

Glu Phe Phe Ala Lys Gly Gin Lys Gly Ser Ser Ala Met Pro His Lys

50 55 60

Arg Asn Pro He Gly Ser Glu Asn Met Thr Gly Leu Ala Arg Val He 65 70 75 80

Arg Gly His Met He Thr Ala Tyr Glu Asn Val Ala Leu Trp His Glu

85 90 95

Arg Asp He Ser His Ser Ser Ala Glu Arg He He Thr Pro Asp Thr

100 105 110

Thr He Leu He Asp Tyr Met Leu Asn Arg Phe Gly Asn He Val Lys

115 120 125

Asn Leu Thr Val Phe Pro Glu Asn Met He Arg Asn Met Asn Ser Thr

130 135 140

Phe Gly Leu He Phe Ser Gin Arg Ala Met Leu Thr Leu He Glu Lys 145 150 155 160

Gly Met Thr Arg Glu Gin Ala Tyr Asp Leu Val Gin Pro Lys Thr Ala

232

SUBSTTTUTE SHEET (RULE 26) 165 170 175

Tyr Ser Trp Asp Asn Gin Val Asp Phe Lys Pro Leu Leu Glu Ala Asp

180 185 190

Ser Glu Val Thr Ser Arg Leu Thr Gin Glu Glu He Asp Glu He Phe

195 200 205

Asn Pro Val Tyr Tyr Thr Lys Arg Val Asp Asp He Phe Glu Arg Leu

210 215 220

Gly Leu Gly Asp 225

(2) INFORMATION FOR SEQ ID NO: 143:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 157 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 143:

Val He Phe He Ser Thr Leu Ser Leu Gly Gly Leu Ala His Leu Leu

1 5 10 15

Trp Phe Ser Leu Pro Leu Ala Ala Cys Leu Ala Val Gly Ala Ala Leu

20 25 30

Gly Pro Thr Asp Leu Val Ala Phe Ala Ser Leu Ser Glu Arg Phe Ser

35 40 45

Phe Pro Lys Arg Val Ser Asn He Leu Lys Gly Glu Gly Leu Leu Asn

50 55 60

Asp Ala Ser Gly Leu Val Ala Phe Gin Val Ala Leu Thr Ala Trp Thr 65 70 75 80

Thr Gly Ala Phe Ser Leu Gly Gin Ala Ser Ser Ser Leu He Phe Ser

85 90 95

He Leu Gly Gly Phe Leu He Gly Phe Leu Thr Ala Met Thr Asn Arg

100 105 110

Phe Leu His Thr Phe Leu Leu Ser Val Arg Ala Thr Asp He Ala Ser

115 120 125

Glu Leu Leu Leu Glu Phe Glu Phe Ala Ser Ser Asp Leu Leu Ser Gly

130 135 140

Arg Arg Ser Pro Cys Phe Arg Asp Tyr Cys Arg Arg Ser 145 150 155

233

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 144:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 230 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 144:

Val Thr Phe Phe Leu Ala Glu Glu Val His Val Ser Gly He He Ala

1 5 10 15

Val Val Val Asp Arg He Leu Lys Ala Ser Arg Phe Lys Lys He Thr

20 25 30

Leu Leu Glu Ala Gin Val Asp Thr Val Thr Glu Thr Val Trp His Thr

35 40 45

Val Thr Phe Met Leu Asn Gly Ser Val Phe Val He Leu Gly Met Glu

50 55 60

Leu Glu Met He Ala Glu Pro He Leu Thr Asn Pro He Tyr Asn Pro 65 70 75 80

Leu Leu Leu Leu Leu Ser Leu He Ala Leu Thr Phe Val Leu Phe Val

85 90 95

He Arg Phe He Met He Tyr Gly Tyr Tyr Ala Tyr Arg Thr Arg Arg

100 105 110

Leu Lys Lys Lys Leu Asn Lys Tyr Met Lys Asp Met Phe Leu Leu Thr

115 120 125

Phe Ser Gly Val Lys Gly Thr Val Ser He Ala Thr He Leu Leu He

130 135 140

Pro Ser Asn Leu Glu Gin Glu Tyr Pro Leu Leu Leu Phe Leu Val Ala 145 150 155 160

Gly Val Thr Leu Val Ser Phe Leu Thr Gly Leu Leu Val Leu Pro His

165 170 175

Leu Ser Asp Glu Glu Glu Glu Ser Lys Asp Tyr Leu Met His He Ala

180 185 190

He Leu Asn Glu Val Thr Leu Glu Leu Glu Lys Glu Leu Glu Asp Thr

195 200 205

Arg Asn Lys Leu Pro Leu Tyr Ala Ala He Asp Asn Ser He Met Asp

210 215 220

Val Leu Lys He Ser Phe 225 230

234

SUBSTTTUTE SHEET (RULE 26) (2) INFORMATION FOR SEQ ID NO: 145:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 98 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 145:

Val Thr Gly Glu Val Gly Asp Leu Lys Gin Gly Phe Ser Val Asn He

1 5 10 15

Glu Val Lys Ser Lys Thr Lys Ala He Leu Val Pro Val Ser Ser Leu

20 25 30

Val Met Asp Asp Ser Lys Asn Tyr Val Trp He Val Asp Glu Gin Gin

35 40 45

Lys Ala Lys Lys Val Glu Val Ser Leu Gly Asn Ala Asp Ala Glu Asn

50 55 60

Gin Glu He Thr Ser Gly Leu Thr Asn Gly Ala Lys Val He Ser Asn 65 70 75 80

Pro Thr Ser Ser Leu Glu Glu Gly Lys Glu Val Lys Ala Asp Glu Ala

85 90 95

Thr Asn

(2) INFORMATION FOR SEQ ID NO: 146:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 182 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 146:

Val Gly Leu Gin He Arg Ala He Phe Lys Arg Tyr Thr Asp Leu He

1 5 10 15

Glu Pro Met Ser He Asp Glu Ala Tyr Leu Asp Val Thr Glu Asn Lys

20 25 30

235

SUBSTTTUTΕ SHEET (RULE 26) Leu Gly He Lys Ser Ala Val Lys He Ala Arg Leu He Gin Lys Asp

35 40 45

He Trp Gin Glu Leu His Leu Thr Ala Ser Ala Gly Val Ser Tyr Asn

50 55 60

Lys Phe Leu Ala Lys Met Ala Ser Asp Tyr Gin Lys Pro His Gly Leu 65 70 75 80

Thr Val He Leu Pro Glu Gin Ala Glu Asp Phe Leu Lys Gin Met Asp

85 90 95

He Ser Lys Phe His Gly Val Gly Lys Lys Thr Val Glu Arg Leu His

100 105 110

Gin Met Gly Val Phe Thr Gly Ala Asp Leu Leu Glu Val Pro Glu Val

115 120 125

Thr Leu He Asp Arg Phe Gly Arg Leu Gly Tyr Asp Leu Tyr Arg Lys

130 135 140

Ala Arg Gly He His Asn Ser Pro Val Lys Ser Asn His He Arg Lys 145 150 155 160

Ser He Gly Lys Glu Lys Thr Tyr Gly Lys He Leu Arg Ala Glu Glu

165 170 175

Asp He Lys Lys Glu Ser 180

(2) INFORMATION FOR SEQ ID NO: 147:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 343 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 147:

Val Asn Leu Pro Lys Arg Ala Phe Leu Asn Gly Arg Val Asp Leu Thr

1 5 10 15

Gin Ala Glu Ala Val Met Asp He He Arg Ala Lys Thr Asp Lys Ala

20 25 30

Met Asn He Ala Val Lys Gin Leu Asp Gly Ser Leu Ser Asp Leu He

35 40 45

Asn Asn Thr Arg Gin Glu He Leu Asn Thr Leu Ala Gin Val Glu Val

50 55 60

Asn He Asp Tyr Pro Glu Tyr Asp Asp Val Glu Glu Ala Thr Thr Ala 65 70 75 80

236

SUBSTTTUTE SHEET (RULE 26) Val Val Arg Glu Lys Thr Met Glu Phe Glu Gin Leu Leu Thr Lys Leu

85 90 95

Leu Arg Thr Ala Arg Arg Gly Lys He Leu Arg Glu Gly He Ser Thr

100 105 110

Ala He He Gly Arg Pro Asn Val Gly Lys Ser Ser Leu Leu Asn Asn

115 120 125

Leu Leu Arg Glu Asp Lys Ala He Val Thr Asp He Ala Gly Thr Thr

130 135 140

Arg Asp Val He Glu Glu Tyr Val Asn He Asn Gly Val Pro Leu Lys 145 150 155 160

Leu He Asp Thr Ala Gly He Arg Glu Thr Asp Asp He Val Glu Gin

165 170 175

He Gly Val Glu Arg Ser Lys Lys Ala Leu Lys Glu Ala Asp Leu Val

180 185 190

Leu Leu Val Leu Asn Ala Ser Glu Pro Leu Thr Ala Gin Asp Arg Gin

195 200 205

Leu Leu Glu He Ser Gin Asp Thr Asn Arg He He Leu Leu Asn Lys

210 215 220

Thr Asp Leu Pro Glu Thr He Glu Thr Ser Lys Leu Pro Glu Asp Val 225 230 235 240

He Arg He Ser Val Leu Lys Asn Gin Asn He Asp Lys He Glu Glu

245 250 255

Arg He Asn Asn Leu Phe Phe Glu Asn Ala Gly Leu Val Glu Gin Asp

260 265 270

Ala Thr Tyr Leu Ser Asn Ala Arg His He Ser Leu He Glu Lys Ala

275 280 285

Val Glu Ser Leu Gin Ala Val Asn Gin Gly Leu Glu Leu Gly Met Pro

290 295 300

Val Asp Leu Leu Gin Val Asp Leu Thr Arg Thr Trp Glu He Leu Gly 305 310 315 320

Glu He Thr Gly Asp Ala Ala Pro Asp Glu Leu He Thr Gin Leu Phe

325 330 335

Ser Gin Phe Cys Leu Gly Lys 340

(2) INFORMATION FOR SEQ ID NO: 148:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 115 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

237

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 148:

Val Glu He Ser Val Gin Pro Pro Gly Lys Lys He Gin Ser Leu Asn

1 5 10 15

Leu Met Ser Gly Gly Glu Lys Ala Leu Ser Ala Leu Ala Leu Leu Phe

20 25 30

Ser He He Arg Val Lys Thr He Pro Phe Val He Leu Asp Glu Val

35 40 45

Glu Ala Ala Leu Asp Glu Ala Asn Val Lys Arg Phe Gly Asp Tyr Leu

50 55 60

Asn Arg Phe Asp Lys Asp Ser Gin Phe He Val Val Thr His Arg Lys 65 70 75 80

Gly Thr Met Ala Ala Ala Asp Ser He Tyr Gly Val Thr Met Gin Glu

85 90 95

Ser Gly Val Ser Lys He Val Ser Val Lys Leu Lys Asp Leu Glu Ser

100 105 110

He Glu Gly 115

(2) INFORMATION FOR SEQ ID NO: 149:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 231 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 149:

Val Thr Thr Val Ala Glu Phe Gly Asp Ser Ser Lys Leu Thr Val Gly

1 5 10 15

Glu Thr Ala He Ala He Gly Ser Pro Leu Gly Ser Glu Tyr Ala Asn

20 25 30

Thr Val Thr Gin Gly He Val Ser Ser Leu Asn Arg Asn Val Ser Leu

35 40 45

Lys Ser Glu Asp Gly Gin Ala He Ser Thr Lys Ala He Gin Thr Asp

50 55 60

Thr Ala He Asn Pro Gly Asn Ser Gly- Gly Pro Leu He Asn He Gin 65 70 75 80

238

SUBSTTTUTΕ SHEET (RULE 26) Gly Gin Val He Gly He Thr Ser Ser Lys He Ala Thr Asn Gly Gly

85 90 95

Thr Ser Val Glu Gly Leu Gly Phe Ala He Pro Ala Asn Asp Ala He

100 105 110

Asn He He Glu Gin Leu Glu Lys Asn Gly Lys Val Thr Arg Pro Ala

115 120 125

Leu Gly He Gin Met Val Asn Leu Ser Asn Val Ser Thr Ser Asp He

130 135 140

Arg Arg Leu Asn He Pro Ser Asn Val Thr Ser Gly Val He Val Arg 145 150 155 160

Ser Val Gin Ser Asn Met Pro Ala Asn Gly His Leu Glu Lys Tyr Asp

165 170 175

Val He Thr Lys Val Asp Asp Lys Glu He Ala Ser Ser Thr Asp Leu

180 185 190

Gin Ser Ala Leu Tyr Asn His Ser He Gly Asp Thr He Lys He Thr

195 200 205

Tyr Tyr Arg Asn Gly Lys Glu Glu Thr Thr Ser He Lys Leu Asn Lys

210 215 220

Ser Ser Gly Asp Leu Glu Ser 225 230

(2) INFORMATION FOR SEQ ID NO: 150:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 40 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 150:

Val Gin Arg Ser Met Leu Leu Pro Gly Gly He Leu Gly Met Thr Val

1 5 10 15

Trp Leu He Tyr Leu Leu Leu Lys Glu Pro Thr Asn Val He Val Ala

20 25 30

Val Asn Gin Ser Leu Lys Arg Ser 35 40

(2) INFORMATION FOR SEQ ID NO: 151:

(i) SEQUENCE CHARACTERISTICS:

239

SUBSTTTUTE SHEET (RULE 26) (A) LENGTH: 102 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 151:

Val Thr Met Glu Leu Asn Thr His Asn Ala Glu He Leu Leu Ser Ala

1 5 10 15

Ala Asn Lys Ser His Tyr Pro Gin Asp Glu Leu Pro Glu He Ala Leu

20 25 30

Ala Gly Arg Ser Asn Val Gly Lys Ser Ser Phe He Asn Thr Met Leu

35 40 45

Asn Arg Lys Asn Leu Ala Arg Thr Ser Gly Lys Pro Gly Lys Thr Gin

50 55 60

Leu Leu Asn Phe Phe Asn He Asp Asp Lys Met Arg Phe Val Asp Val 65 70 75 80

Pro Gly Tyr Gly Tyr Ala Arg Val Ser Lys Lys Glu Arg Glu Lys Trp

85 90 95

Gly Cys Met He Glu Glu 100

(2) INFORMATION FOR SEQ ID NO: 152:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 70 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 152:

Val Gin Met Tyr Glu Phe Leu Lys Tyr Tyr Glu He Pro Val He He

1 5 10 15

Val Ala Thr Lys Ala Asp Lys He Pro Arg Gly Lys Trp Asn Lys His

20 25 30

Glu Ser Ala He Lys Lys Lys Leu Asn Phe Asp Pro Ser Asp Asp Phe

35 40 45

He Leu Phe Ser Ser Val Ser Lys Ala Gly Met Asp Glu Ala Trp Asp

240

SUBSTTTUTΕ SHEET (RULE 26) 50 55 60

Ala He Leu Glu Lys Leu 65 70

(2) INFORMATION FOR SEQ ID NO: 153:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 59 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:153:

Val Phe Met Val Tyr Asn Cys Pro Lys Pro Val Tyr Ser Phe Leu Lys

1 5 10 15

Ser Ala He Asn Leu Met Ala Ala He Pro Ser He Val Tyr Gly Phe

20 25 30

Phe Gly Leu Gin Leu Leu Val Pro Trp He Lys Thr Phe Leu Gly Asn

35 40 45

Gly Met Ser Cys Pro Asn Gin Leu Arg Tyr Tyr 50 55

(2) INFORMATION FOR SEQ ID NO: 154:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 294 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 154:

Val He He Met Lys Phe Lys Lys Met Leu Thr Leu Ala Ala He Gly

1 5 10 15

Leu Ser Gly Phe Gly Leu Val Ala Cys Gly Asn Gin Ser Ala Ala Ser

20 25 30

Lys Gin Ser Ala Pro Gly Thr He Glu Val He Ser Arg Glu Asn Gly

35 40 45

241

SUBSTTTUTE SHEET (RULE 26) Ser Gly Thr Arg Gly Ala Phe Thr Glu He Thr Gly He Leu Lys Lys

50 55 60

Asp Gly Asp Lys Lys He Asp Tyr Thr Ala Lys Thr Ala Val He Gin 65 70 75 80

Asn Ser Thr Glu Gly Val Leu Ser Ala Val Gin Gly Asn Ala Asn Ala

85 90 95

He Gly Tyr He Ser Leu Gly Ser Leu Thr Lys Ser Val Lys Ala Leu

100 105 110

Glu He Asp Gly Val Lys Ala Ser Arg Asp Thr Val Leu Asp Gly Glu

115 120 125

Tyr Pro Leu Gin Arg Pro Phe Asn He Val Trp Ser Ser Asn Leu Ser

130 135 140

Lys Leu Gly Gin Asp Phe He Ser Phe He His Ser Lys Gin Gly Gin 145 150 155 160

Gin Val Val Thr Asp Asn Lys Phe He Glu Ala Lys Thr Glu Thr Thr

165 170 175

Glu Tyr Thr Ser Gin His Leu Ser Gly Lys Leu Ser Val Val Gly Ser

180 185 190

Thr Ser Val Ser Ser Leu Met Glu Lys Leu Ala Glu Ala Tyr Lys Lys

195 200 205

Glu Asn Pro Glu Val Thr He Asp He Thr Ser Asn Gly Ser Ser Ala

210 215 220

Gly He Thr Ala Val Lys Glu Lys Thr Ala Asp He Gly Met Val Ser 225 230 235 240

Arg Glu Leu Thr Pro Glu Glu Gly Lys Ser Leu Thr His Asp Ala He

245 250 255

Ala Leu Asp Gly He Ala Val Val Val Asn Asn Asp Asn Lys Ala Ser

260 265 270

Gin Val Ser Met Ala Glu Leu Ala Asp Val Phe Ser Gly Lys Leu Thr

275 280 285

Thr Trp Asp Lys He Lys 290

(2) INFORMATION FOR SEQ ID NO: 155:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 158 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 155:

Val Ser Ser He Leu Gly Ala Gly Pro Phe Phe Gly Leu Ala His Glu

1 5 10 15

Ala Gin Leu Lys He Leu Glu Leu Thr Ala Gly Gin Val Ala Thr Met

20 25 30

Tyr Glu Ser Pro Val Gly Phe Arg His Gly Pro Lys Ser Leu He Asn

35 40 45

Asp Asn Thr Val Val Leu Val Phe Gly Thr Thr Thr Asp Tyr Thr Arg

50 55 60

Lys Tyr Asp Leu Asp Leu Val Arg Glu Val Ala Gly Asp Gin He Ala 65 70 75 80

Arg Arg Val Val Leu Leu Ser Asp Gin Ala Phe Gly Leu Glu Asn Val

85 90 95

Lys Glu Val Ala Leu Gly Cys Gly Gly Val Leu Asn Asp He Tyr Arg

100 105 110

Val Phe Pro Tyr He Val Tyr Ala Gin Leu Phe Ala Leu Leu Thr Ser

115 120 125

Leu Lys Val Glu Asn Lys Pro Asp Thr Pro Ser Pro Thr Gly Thr Val

130 135 140

Asn Arg Val Val Gin Gly Val He He His Glu Tyr Gin Lys 145 150 155

(2) INFORMATION FOR SEQ ID NO: 156:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 271 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 156:

Val Lys Pro Gly Asp Phe Val He Val Pro Phe Thr His Gly Cys Gly

1 5 10 15

Glu Cys Asp Ala Cys Leu Ala Gly Phe Asp Gly Ser Cys Asp Asn His

20 25 30

He Gly Asn Asn Leu Gly Gly Asp Phe Gin Ala Glu Tyr He Arg Phe

35 40 45

His Tyr Ala Asn Trp Ala Leu Val Lys He Pro Gly Gin Pro Ser Asp 50 55 60

243

SUBSTTTUTE SHEET (RULE 26) Tyr Thr Glu Gly Met Leu Lys Ser Leu Leu Thr Leu Ala Asp Val Met 65 70 75 80

Pro Thr Gly Tyr His Ala Ala Arg Val Ala Asn Val Gin Lys Gly Asp

85 90 95

Lys Val Val Val He Gly Asp Gly Ala Val Gly Gin Cys Ala Val He

100 105 110

Ala Ala Lys Met Arg Gly Ala Ser Gin He He Leu Met Ser Arg His

115 120 125

Glu Asp Arg Gin Lys Met Ala Met Glu Ser Gly Ala Thr Ala Val Val

130 135 140

Ala Glu Arg Gly Gin Glu Gly He Thr Lys Val Arg Glu He Leu Gly 145 150 155 160

Gly Gly Ala Asp Ala Ala Leu Glu Cys Val Gly Thr Glu Ala Ala He

165 170 175

Glu Gin Ala Leu Gly Val Leu His Asn Gly Gly Arg Met Gly Phe Val

180 185 190

Gly Val Pro His Tyr Asn Asn Arg Ala Leu Gly Ser Thr Phe Met Gin

195 200 205

Asn He Ser Val Ala Gly Gly Ala Ala Ser Ala Thr Thr Tyr Asp Lys

210 215 220

Gin Phe Leu Leu Lys Ala Val Leu Asp Gly Asp He Asn Pro Gly Arg 225 230 235 240

Val Phe Thr Ser Ser Tyr Lys Leu Glu Asp He Asp Gin Ala Tyr Lys

245 250 255

Asp Met Asp Glu Arg Lys Thr He Lys Ser Met He Val He Glu 260 265 270

(2) INFORMATION FOR SEQ ID NO: 157:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 122 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 157:

Val Arg Lys Ser Arg Val Asn Asn Ser Gin Gin Met Leu Gin Ala Leu

1 5 10 15

Glu Glu Gin Asp Leu Thr Lys Ala Glu His Tyr Phe Ala Lys Ala Leu 20 25 30

244

SUBSTTTUTE SHEET (RULE 26) Glu Asn Asp Ser Ser Asp Leu Leu Tyr Glu Leu Ala Thr Tyr Leu Glu

35 40 45

Gly He Gly Phe Tyr Pro Gin Ala Lys Glu He Tyr Leu Lys He Val

50 55 60

Glu Glu Phe Pro Glu Val His Leu Asn Leu Ala Ala Met Ala Ser Glu

65 70 75 80

Asp Gly Gin He Glu Lys Ala Phe Asn Tyr Leu Glu Glu He Gin Ala

85 90 95

Asp Ser Asp Trp Tyr Val Ser Leu Phe Gly Ser Glu Gly Arg Pro He

100 105 110

Pro Ala Gly Arg Phe Asp Arg Cys Gly Thr 115 120

(2) INFORMATION FOR SEQ ID NO: 158:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 317 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 158:

Val Thr Gly Met Ser Arg Ser Leu Ala Leu Lys Ala Asp Leu Tyr Gin

1 5 10 15

Leu Glu Gly Leu Thr Asp Val Ala Arg Glu Lys Leu Leu Glu Ala Leu

20 25 30

Thr Tyr Ser Lys Asp Ser Leu Leu He Leu Gly Leu Ala Lys Leu Asp

35 40 45

Ser Glu Leu Glu Asn Tyr Gin Ala Ala He Gin Ala Tyr Ala Gin Leu

50 55 60

Asp Asn Arg Ser He Tyr Glu Gin Thr Gly He Ser Thr Tyr Gin Arg 65 70 75 80

He Gly Phe Ala Tyr Ala Gin Leu Gly Lys Phe Glu Thr Ala Thr Glu

85 90 95

Phe Leu Glu Lys Ala Leu Glu Leu Glu Tyr Asp Asp Leu Thr Ala Phe

100 105 110

Glu Leu Ala Ser Leu Tyr Phe Asp Gin Glu Glu Tyr Gin Lys Ala Thr

115 120 125

Leu Tyr Phe Lys Gin Leu Asp Thr He -Ser Pro Asp Phe Glu Gly Tyr 130 135 140

245

SUBSTTTUTE SHEET (RULE 26) Glu Tyr Gly Tyr Ser Gin Ala Leu His Lys Glu His Gin Val Gin Glu 145 150 155 160

Ala Leu Arg He Ala Lys Gin Gly Leu Glu Lys Asn Pro Phe Glu Thr

165 170 175

Arg Leu Leu Leu Ala Ala Ser Gin Phe Ser Tyr Glu Leu His Asp Ala

180 185 190

Ser Gly Ala Glu Asn Tyr Leu Leu Thr Ala Lys Glu Asp Ala Glu Asp

195 200 205

Thr Glu Glu He Leu Leu Arg Leu Ala Thr He Tyr Leu Glu Gin Glu

210 215 220

Arg Tyr Glu Asp He Leu Asp Leu Gin Ser Glu Glu Pro Glu Asn Leu 225 230 235 240

Leu Thr Lys Trp Met He Ala Arg Ser Tyr Gin Glu Met Asp Asp Leu

245 250 255

Asp Thr Ala Tyr Glu His Tyr Gin Glu Leu Thr Gly Asp Leu Lys Asp

260 265 270

Asn Pro Glu Phe Leu Glu His Tyr He Tyr Leu Leu Arg Glu Leu Gly

275 280 285

His Phe Glu Glu Ala Lys Val His Ala His Thr Tyr Leu Lys Leu Val

290 295 300

Pro Asp Asp Val Gin Met Gin Glu Leu Phe Glu Arg Leu 305 310 315

(2) INFORMATION FOR SEQ ID NO: 159:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 77 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 159:

Val Glu Lys Ala Gly Val Val He Ala He Asn His Asn Glu He Pro

1 5 10 15

Trp Glu Thr He Asp Gly Lys Gly Val Lys Val He Val Leu Phe Ala

20 25 30

Val Gly Asp Asp Thr Glu Ala Ala Arg Glu His Leu Lys Thr Leu Ser

35 40 45

Leu Phe Ala Arg Lys Leu Gly Asn Asp -Glu Val Val Ala Lys Leu Val 50 55 60

246

SUBSTTTUTE SHEET (RULE 26) Arg Ala Gin Thr Ser Asp Asp Val He Ala Ala Phe Cys 65 70 75

(2) INFORMATION FOR SEQ ID NO: 160:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 46 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 160:

Val Ser Asp Phe His Asp Phe Ser Asp Arg Glu Val Arg Trp Leu Ser

1 5 10 15

Pro Glu Glu Phe Lys Asn Tyr Pro Leu Ala Lys Pro Gin Gin Lys He

20 25 30

Trp Gin Ala Tyr Ala Gin Ala Asn Leu Asp Ser Ser Gin Asp 35 40 45

(2) INFORMATION FOR SEQ ID NO: 161:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 96 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 161:

Val Asn Phe Glu Lys Lys Ala Gin Thr Gin He Ala Gin He Val Gin

1 5 10 15

Asn Gly Trp Asp Lys Leu Pro He Cys Met Ala Lys Thr Gin Tyr Ser

20 25 30

Phe Ser Asp Asn Pro Asn Ala Leu Gly Ala Pro Glu Asn Phe Glu He

35 40 45

Thr He Arg Glu Leu Val Pro Lys Leu Gly Ala Gly Phe He Val Ala

50 55 60

Leu Thr Gly Asp Val Met Thr Met Pro Gly Leu Pro Lys Arg Pro Ala

247

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Ala Leu Asn Met Asp Val Glu Ser Asp Gly Thr Val Leu Gly Leu Phe 85 90 95

(2) INFORMATION FOR SEQ ID NO: 162:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 292 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:162:

Val Lys Lys Arg Lys Lys Leu Ala Leu Ser Leu He Ala Phe Trp Leu

1 5 10 15

Thr Ala Cys Leu Val Gly Cys Ala Ser Trp He Asp Arg Gly Glu Ser

20 25 30

He Thr Ala Val Gly Ser Thr Ala Leu Gin Pro Leu Val Glu Val Ala

35 40 45

Ala Asp Glu Phe Gly Thr He His Val Gly Lys Thr Val Asn Val Gin

50 55 60

Gly Gly Ser Ser Gly Thr Gly Leu Ser Gin Val Gin Ser Gly Ala Val 65 70 75 80

Asp He Gly Asn Ser Asp Val Phe Ala Glu Glu Lys Asp Gly He Asp

85 90 95

Ala Ser Ala Leu Val Asp His Lys Val Ala Val Ala Gly Leu Ala Leu

100 105 110

He Val Asn Lys Glu Val Asp Val Asp Asn Leu Thr Thr Glu Gin Leu

115 120 125

Arg Gin He Phe He Gly Glu Val Thr Asn Trp Lys Glu Val Gly Gly

130 135 140

Lys Asp Leu Pro He Ser Val He Asn Arg Ala Ala Gly Ser Gly Ser 145 150 155 160

Arg Ala Thr Phe Asp Thr Val He Met Glu Gly Gin Ser Ala Met Gin

165 170 175

Ser Gin Glu Gin Asp Ser Asn Gly Ala Val Lys Ser He Val Ser Lys

180 185 190

Ser Pro Gly Ala He Ser Tyr Leu Ser Leu Thr Tyr He Asp Asp Ser

195 200 205

Val Lys Ser Met Lys Leu Asn Gly Tyr Asp Leu Ser Pro Glu Asn He

248

SUBSTTTUTE SHEET (RULE 26) 210 215 220

Ser Ser Asn Asn Trp Pro Leu Trp Ser Tyr Glu His Met Tyr Thr Leu 225 230 235 240

Gly Gin Pro Asn Glu Leu Ala Ala Glu Phe Leu Asn Phe Val Leu Ser

245 250 255

Asp Glu Thr Gin Glu Gly He Val Lys Gly Leu Lys Tyr He Pro He

260 265 270

Lys Glu Met Lys Val Glu Lys Asp Ala Ala Gly Thr Val Thr Val Leu

275 280 285

Glu Gly Arg Gin 290

(2) INFORMATION FOR SEQ ID NO:163:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 71 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 163:

Val Gin Pro Thr Gin Ala Glu Gin Pro Ser Thr Pro Lys Glu Ser Ser

1 5 10 15

Gin Gin Glu Asn Pro Lys Glu Asp Arg Gly Ala Glu Glu Thr Pro Lys

20 25 30

Gin Glu Asp Glu Gin Pro Ala Glu Ala Gin Glu He Lys Val Glu Glu

35 40 45

Pro Val Glu Ser He Glu Glu Thr Val He Gin Pro Val Glu Gin Pro

50 55 60

Lys Val Glu Thr Pro Ala Val 65 70

(2) INFORMATION FOR SEQ ID NO: 164:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 465 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

249

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 164:

Val Leu Leu Lys Met Asp Gly Tyr Arg Tyr Val Gly Tyr Leu Ser Gly

1 5 10 15

Asp He Leu Lys Thr Leu Gly Leu Asp Thr Val Leu Glu Glu Thr Ser

20 25 30

Ala Lys Pro Gly Glu Val Thr Val Val Glu Val Glu Thr Pro Gin Ser

35 40 45

Thr Thr Asn Gin Glu Gin Ala Arg Thr Glu Asn Gin Val Val Glu Thr

50 55 60

Glu Glu Ala Pro Lys Glu Glu Ala Pro Lys Thr Glu Glu Ser Pro Lys 65 70 75 80

Glu Glu Pro Lys Ser Glu Val Lys Pro Thr Asp Asp Thr Leu Pro Lys

85 90 95

Val Glu Glu Gly Lys Glu Asp Ser Ala Glu Pro Ser Pro Val Glu Glu

100 105 110

Val Gly Gly Glu Val Glu Ser Lys Pro Glu Glu Lys Val Ala Val Lys

115 120 125

Pro Glu Ser Gin Pro Ser Asp Lys Pro Ala Glu Glu Ser Lys Val Glu

130 135 140

Pro Pro Val Glu Gin Ala Lys Val Pro Glu Gin Pro Val Gin Pro Thr 145 ^'l50 155 160

Gin Ala Glu Gin Pro Ser Thr Pro Lys Glu Ser Ser Gin Gin Glu Asn

165 170 175

Pro Lys Glu Asp Arg Gly Ala Glu Glu Thr Pro Lys Gin Glu Asp Glu

180 185 190

Gin Pro Ala Glu Ala Gin Glu He Lys Val Glu Glu Pro Val Glu Ser

195 200 205

Lys Glu Glu Thr Val Asn Gin Pro Val Glu Gin Pro Lys Val Glu Thr

210 215 220

Pro Ala Val Glu Lys Gin Thr Glu Pro Thr Glu Glu Pro Lys Val Glu 225 230 235 240

Val Thr Ser He Pro Gin Thr Thr Arg Tyr Glu Glu Asp Leu Thr Lys

245 250 255

Glu His Gly Thr Arg Glu Val Val Lys Glu Gly Lys Asn Gly Ser Arg

260 265 270

Thr Val Thr Thr Pro Tyr He Leu Asn Ala Thr Asp Gly Thr Thr Thr

275 280 285

Glu Gly Thr Ser Thr Thr Asp Glu Ala Glu Met Glu Lys Glu Val Val

290 295 300

Arg Val Gly Thr Lys Pro Lys Glu Lys Leu Ala Pro Val Leu Ser Leu

250

SUBSTTTUTE SHEET (RULE 26) 305 310 315 320

Thr Ser Val Thr Asp Asn Ala Met Leu Arg Ser Ala Arg Leu Thr Tyr

325 330 335

His Leu Glu Asn Thr Asp Ser Val Asp Val Lys Lys He His Ala Glu

340 345 350

He Lys Asn Gly Asp Lys Val Val Lys Thr He Asp Leu Ser Lys Glu

355 360 365

Arg Leu Ser Asp Ala Val Asp Gly Leu Glu Leu Tyr Lys Asp Tyr Lys

370 375 380

He Val Thr Ser Met Thr Tyr Asp Arg Gly Asn Gly Glu Glu Thr Ser 385 390 395 400

Thr Leu Glu Glu Thr Pro Leu Arg Leu Asp Leu Lys Lys Val Glu Leu

405 410 415

Lys Asn He Gly Ser Thr Asn Leu Val Lys Val Asn Glu Asp Gly Thr

420 425 430

Glu Val Ala Ser Asp Phe Leu Thr Ser Lys Pro Val Asp Val Gin Asn

435 440 445

Tyr Tyr Leu Lys Val Thr Ser Arg Asp Asn Lys Val Val Ser Pro Pro

450 455 460

Ser 465

(2) INFORMATION FOR SEQ ID NO: 165:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 152 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 165:

Val Gin Leu Tyr Lys Ala Trp Ser Glu He Gly Ser Val Val His Thr

1 5 10 15

His Ser Thr Glu Ala Val Ala Trp Ala Gin Ala Gly Arg Asp He Pro

20 25 30

Phe Tyr Gly Thr Thr His Ala Asp Tyr Phe Tyr Gly Ser He Pro Cys

35 40 45

Ala Arg Ser Leu Thr Lys Asp Glu Val Glu Val Ala Tyr Glu Lys Asp

50 55 60

Thr Gly Leu Val He Val Glu Glu Phe Glu His Arg Gly Leu Asn Pro

251

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Val Glu Val Pro Gly He Val Val Arg Asn His Gly Pro Phe Thr Trp

85 90 95

Gly Lys Asn Pro Glu Asn Ala Val Tyr His Ser Val Val Leu Glu Glu

100 105 110

Val Ser Lys Met Asn Arg Phe Thr Glu Gin He Asn Pro Arg Val Glu

115 120 125

Pro Ala Pro Gin Tyr He Leu Glu Lys His Tyr Gin Arg Lys His Gly

130 135 140

Pro Asn Ala Tyr Tyr Gly Gin Lys 145 150

(2) INFORMATION FOR SEQ ID NO: 166:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 74 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 166:

Val Val Lys Ala He Gin Asp Gly Lys Ala Lys Leu Val Phe Leu Ala

1 5 10 15

His Asp Ala Gly Pro Asn Leu Thr Lys Lys He Gin Asp Lys Ser His

20 25 30

Tyr Tyr Gin Val Glu He Val Thr Val Phe Ser Thr Leu Glu Leu He

35 40 45

He Ala Val Gly Lys Ser Arg Lys Val Leu Ala Val Thr Asp Ala Gly

50 55 60

Phe Thr Lys Lys Met Arg Ser Leu Met Glu 65 70

(2) INFORMATION FOR SEQ ID NO: 167:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 190 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

252

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 167:

Val Ala Asp Asp Asp Gin Cys He Phe Leu Cys His Asn His Arg Ala

1 5 10 15

Gin Glu Ser He Glu Phe Glu Lys Met He Asp Gin Leu Ser Lys Tyr

20 25 30

Tyr Ser Cys Arg He Leu Thr Glu Lys Asp He Pro Ser He Leu Ser

35 40 45

Leu Tyr Glu Ser Asn Pro Leu Tyr Phe Gin His Cys Pro Pro Glu Pro

50 55 60

Asn Phe Ala Thr Val Lys Glu Asp Met Leu Cys Leu Pro Glu Gly Lys 65 70 75 80

Ala Lys Ala Asp Lys Phe Phe Val Gly Phe Trp Asn Gly Phe Asp Leu

85 90 95

Val Ala Val Met Asp Phe Val Tyr Ala Tyr Pro Asp Glu Glu Thr Val

100 105 110

Phe He Gly Leu Phe Met Val Asp Gin Ala Tyr Gin Arg Lys Gly He

115 120 125

Gly Ser His He Val Thr Glu Ala Leu Ala Tyr Phe Ala Lys Asn Phe

130 135 140

Arg Lys Ala Arg Leu Ala Tyr Val Lys Gly Asn Pro Gin Ser Gin His 145 150 155 160

Phe Trp Glu Lys Gin Gly Phe Lys Ser He Gly Cys Glu Val Lys Gin

165 170 175

Glu Leu Tyr Thr Val Val He Val Glu Gin Ser Leu Glu Asp 180 185 190

(2) INFORMATION FOR SEQ ID NO: 168:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 215 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 168:

Val Ala Leu Thr Pro Leu Leu Lys Glu Glu Gly Val Ala Asp He Pro 1 5 10 15

253

SUBSTTTUTE SHEET (RULE 26) Ala Tyr Lys Asp Tyr Tyr Val Pro Met Asn Lys Ala Leu Trp Lys Asp

20 25 30

Leu Glu Leu Lys Lys He Ser Lys Gin Glu Leu Val Asn Thr Arg Phe

35 40 45

Ser Arg Leu Phe Ala His Phe Gly Gin Glu Lys Asp Gly Ser Phe Leu

50 55 60

Ala Gin Arg Tyr Gin Phe Tyr Leu Ala Gin Gin Gly Gin Thr Leu Ser 65 70 75 80

Gly Ala His Asp Leu Leu Asp Ser Leu He Glu Arg Asp Tyr Asn Leu

85 90 95

Tyr Ala Ala Thr Asn Gly He Thr Ala He Gin Thr Gly Arg Leu Ala

100 105 110

Gin Ser Gly Leu Ala Pro Tyr Phe Asn Gin Val Phe He Ser Glu Gin

115 120 125

Leu Gin Thr Gin Lys Pro Asp Ala Leu Phe Tyr Glu Lys He Gly Gin

130 135 140

Gin He Ala Gly Phe Ser Lys Glu Lys Thr Leu Met He Gly Asp Ser 145 150 155 160

Leu Thr Ala Asp He Gin Gly Gly Asn Asn Ala Gly He Asp Thr He

165 170 175

Trp Tyr Asn Pro His His Leu Glu Asn His Thr Gin Ala Gin Pro Thr

180 185 190

Tyr Glu Val Tyr Ser Tyr Gin Asp Leu Leu Asp Cys Leu Asp Lys Asn

195 200 205

He Leu Glu Lys He Thr Phe 210 215

(2) INFORMATION FOR SEQ ID NO: 169:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 299 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 169:

Val Ala Ala Leu Ser Gin Gin Asp Val Pro Lys A] a Leu Ser Cys Leu

1 5 10 15

Asn Leu Leu Phe Asp Asn Gly Lys Ser Met Thr Arg Phe Val Thr Asp 20 25 30

254

SUBSTTTUTE SHEET (RULE 26) Leu Leu His Tyr Leu Arg Asp Leu Leu He Val Gin Thr Gly Gly Glu

35 40 45

Asn Thr His His Ser Ser Val Phe Val Glu Asn Leu Ala Leu Pro Gin

50 55 60

Lys Asn Leu Phe Glu Met He Arg Leu Ala Thr Val Asn Leu Ala Asp 65 70 75 80

He Lys Ser Ser Leu Gin Pro Lys He Tyr Ala Glu Met Met Thr Val

85 90 95

Arg Leu Ala Glu He Lys Pro Glu Pro Ala Leu Ser Gly Ala Val Glu

100 105 110

Asn Arg He Ala Thr Leu Arg Gin Glu Val Ala Arg Leu Lys Gin Glu

115 120 125

Leu Ser Asn Ala Gly Ala Val Pro Lys Gin Val Ala Pro Ala Pro Ser

130 135 140

Arg Pro Ala Thr Gly Lys Thr Val Tyr Arg Val Asp Arg Asn Lys Val 145 150 155 160

Gin Ser He Leu Gin Glu Ala Val Glu Asn Pro Asp Leu Ala Arg Gin

165 170 175

Asn Leu He Arg Leu Gin Asn Ala Trp Gly Glu Val He Glu Ser Leu

180 185 190

Gly Gly Pro Asp Lys Ala Leu Leu Val Gly Ser Gin Pro Val Ala Ala

195 200 205

Asn Glu His His Ala He Leu Ala Phe Glu Ser Asn Phe Asn Ala Gly

210 215 220

Gin Thr Met Lys Arg Asp Asn Leu Asn Thr Met Phe Gly Asn He Leu 225 230 235 240

Ser Gin Ala Ala Gly Phe Ser Pro Glu He Leu Ala He Ser Met Glu

245 250 255

Glu Trp Lys Glu Val Arg Ala Ala Phe Ser Ala Lys Ala Lys Ser Ser

260 265 270

Gin Thr Glu Lys Glu Val Glu Glu Ser Leu He Pro Glu Gly Phe Glu

275 280 285

Phe Leu Ala Asp Lys Val Lys Val Glu Glu Asp 290 295

(2) INFORMATION FOR SEQ ID NO: 170:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 147 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

255

SUBSTTTUTE SHEET (RULE 26) (ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 170:

Val Pro Leu Val He Leu Met He Gly Met Leu Ala Gly Ser He Ser

1 5 10 15

His Gin Val Met His Trp Gly Thr Phe Leu Ala Thr Thr Pro He Met

20 25 30

Leu Val Ala Gly Lys Pro Tyr He Gin Ser Ala Trp Ala Ser Phe Lys

35 40 45

Lys His Asn Ala Asn Met Asp Thr Leu Val Ala Leu Gly Thr Leu Val

50 55 60

Ala Tyr Phe Tyr Ser Leu Val Ala Leu Phe Ala Gly Leu Pro Val Tyr 65 70 75 80

Phe Glu Ser Ala Gly Phe He Leu Phe Phe Val Leu Leu Gly Ala Val

85 90 95

Phe Glu Glu Lys Met Arg Lys Asn Thr Ser Gin Ala Val Glu Lys Leu

100 105 110

Leu Asp Leu Gin Ala Lys Thr Ala Glu Val Leu Ser Asp Asp Ser Tyr

115 120 125

Val Gin Val Pro Leu Glu Gin Val Lys Val Arg Asp Leu Asp Ser Ser

130 135 140

Ala Ser Arg 145

(2) INFORMATION FOR SEQ ID NO: 171:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 73 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 171:

Val Thr Glu Asn Ala Glu Ala Ala Ala Tyr Phe Thr Asp Gin Val Asp

1 5 10 15

Ser Ala Ala Val Tyr Val Asn Ala Ser Thr Arg Phe Thr Asp Gly Gly

20 25 30

Gin Phe Gly Leu Gly Cys Glu Met Gly Tie Ser Thr Gin Lys Leu His 35 40 45

256

SUBSTTTUTE SHEET (RULE 26) Ala Arg Gly Pro Met Gly Leu Lys Glu Leu Thr Ser Tyr Lys Tyr Val

50 55 60

Val Ala Gly Asp Gly Gin He Arg Glu 65 70

(2) INFORMATION FOR SEQ ID NO: 172:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 94 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 172:

Val Asp Leu Pro Gin Gin Phe His Leu Gly Ser He Thr Lys Thr Phe

1 5 10 15

Gin Trp Leu Val Asp He Asn Asn Leu Val Phe Lys Gly Ser He Pro

20 25 30

He Val Ser Leu Leu Phe He Tyr Cys Leu Gly Val Asn He Ala Lys

35 40 45

He Tyr Lys Val Asp Thr Val Ser Ala Gly Leu Val Ser Leu Ala Ser

50 55 60

Phe Val He Ser He Gly Ser Thr Val Thr Lys Ser Phe Pro Leu Ala

65 70 75 80

Asn Val Gly Asp Val Lys Leu Asp Gin He Leu Thr Trp Asn 85 90

(2) INFORMATION FOR SEQ ID NO:173:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 330 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 173:

Val Ser Leu Arg Leu He Tyr Ser He Phe Lys Lys Met Arg Lys Asn

257

SUBSTTTUTE SHEET (RULE 26) 1 5 10 15

Met Lys He Ser His Met Lys Lys Asp Glu Leu Phe Glu Gly Phe Tyr

20 25 30

Leu He Lys Ser Ala Asp Leu Arg Gin Thr Arg Ala Gly Lys Asn Tyr

35 40 45

Leu Ala Phe Thr Phe Gin Asp Asp Ser Gly Glu He Asp Gly Lys Leu

50 55 60

Trp Asp Ala Gin Pro His Asn He Glu Ala Phe Thr Ala Gly Lys Val 65 70 75 80

Val His Met Lys Gly Arg Arg Glu Val Tyr Asn Asn Thr Pro Gin Val

85 90 95

Asn Gin He Thr Leu Arg Leu Pro Gin Ala Gly Glu Pro Asn Asp Pro

100 105 110

Ala Asp Phe Lys Val Lys Ser Pro Val Asp Val Lys Glu He Arg Asp

115 120 125

Tyr Met Ser Gin Met He Phe Lys He Glu Asn Pro Val Trp Gin Arg

130 135 140

He Val Arg Asn Leu Tyr Thr Lys Tyr Asp Lys Glu Phe Tyr Ser Tyr 145 150 155 160

Pro Ala Ala Lys Thr Asn His His Ala Phe Glu Thr Gly Leu Ala Tyr

165 170 175

His Thr Ala Thr Met Val Arg Leu Ala Asp Ala He Ser Glu Val Tyr

180 185 190

Pro Gin Leu Asn Lys Ser Leu Leu Tyr Ala Gly He Met Leu His Asp

195 200 205

Leu Ala Lys Val He Glu Leu Thr Gly Pro Asp Gin Thr Glu Tyr Thr

210 215 220

Val Arg Gly Asn Leu Leu Gly His He Ala Leu He Asp Ser Glu He 225 230 235 240

Thr Lys Thr Val Met Glu Leu Gly He Asp Asp Thr Lys Glu Glu Val

245 250 255

Val Leu Leu Arg His Val He Leu Lys Ser Thr Thr Ala Cys Leu Asn

260 265 270

Met Glu He Pro Val Arg Pro Arg He Met Glu Ala Glu He He His

275 280 285

Met He Asp Asn Leu Asp Ala Ser Met Met Met Met Ser Thr Ala Leu

290 295 300

Ala Leu Val Asp Lys Gly Glu Met Thr Asn Lys He Phe Ala Met Asp 305 310 315 320

Asn Arg Ser Phe Tyr Lys Pro Asp Leu Asp 325 330

(2) INFORMATION FOR SEQ ID NO: 174:

258

SUBSTTTUTE SHEET (RULE 26) (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 137 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 174:

Val Trp Lys Lys Lys Lys Val Lys Ala Gly Val Leu Leu Tyr Ala Val

1 5 10 15

Thr He Ala Ala He Phe Ser Leu Leu Leu Gin Phe Tyr Leu Asn Arg

20 25 30

Gin Val Ala His Tyr Gin Asp Tyr Ala Leu Asn Lys Glu Lys Leu Val

35 40 45

Ala Phe Ala Met Ala Lys Arg Thr Lys Asp Lys Val Glu Gin Glu Ser

50 55 60

Gly Glu Gin Val Phe Asn Leu Gly Gin Val Ser Tyr Gin Asn Lys Lys 65 70 75 80

Thr Gly Leu Val Thr Arg Val Arg Thr Asp Lys Ser Gin Tyr Glu Phe

85 90 95

Leu Phe Pro Ser Val Lys He Lys Glu Glu Lys Arg Asp Lys Lys Glu

100 105 110

Glu Val Ala Thr Asp Ser Ser Glu Lys Val Glu Lys Lys Lys Ser Glu

115 120 125

Glu Lys Pro Glu Lys Lys Glu Asn Ser 130 135

(2) INFORMATION FOR SEQ ID NO: 175:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 163 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 175:

Val Asp Gly Lys Phe Gly Lys His Val Glu Gin He Pro Glu Gly Ala

259

SUBSTTTUTE SHEET (RULE 26) 1 5 10 15

Glu Val He Asp Tyr Thr Gly Tyr Ser He Ala Pro Gly Leu Val Asp

20 25 30

Thr His He His Gly Tyr Ala Gly Val Asp Val Met Asp Asn Asn He

35 40 45

Glu Gly Thr Leu His Thr Met Ser Glu Gly Leu Leu Ser Thr Gly Val

50 55 60

Thr Ser Phe Leu Pro Thr Thr Leu Thr Ala Thr Tyr Glu Gin Leu Leu 65 70 75 80

Ala Val Thr Glu Asn Leu Gly Asn His Tyr Lys Glu Ala Thr Gly Ala

85 90 95

Lys He Arg Gly He Tyr Tyr Glu Gly Pro Tyr Phe Thr Glu Thr Phe

100 105 110

Lys Gly Ala Gin Asn Pro Thr Tyr Met Arg Asp Pro Gly Val Glu Glu

115 120 125

Phe His Ser Trp Gin Lys Ala Ala Asn Gly Leu Leu Asn Lys He Arg

130 135 140

Leu His Gin Asn Val Met Gly Trp Lys Thr Leu Phe Val Gin Leu Arg 145 150 155 160

Ala Lys Val

(2) INFORMATION FOR SEQ ID NO: 176:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 234 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 176:

Val Arg Arg He Glu Glu Lys Cys Lys Leu He Ala Gin Leu Asp Thr

1 5 10 15

Lys Thr Val Tyr Ser Phe Met Glu Ser Val He Ser He Glu Lys Tyr

20 25 30

Val Arg Ala Ala Lys Glu Tyr Gly Tyr Thr His Leu Ala Met Met Asp

35 40 45

He Asp Asn Leu Tyr Gly Ala Phe Asp Phe Leu Glu He Thr Lys Lys

50 55 60

Tyr Gly He His Pro Leu Leu Gly Leu Glu Met Thr Val Phe Val Asp

260

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Asp Gin Glu Val Asn Leu Arg Phe Leu Ala Leu Ser Ser Val Gly Tyr

85 90 95

Gin Gin Leu Met Lys Leu Ser Thr Ala Lys Met Gin Gly Glu Lys Thr

100 105 110

Trp Ser Val Leu Ser Gin Tyr Leu Glu Asp He Ala Val He Val Pro

115 120 125

Tyr Phe Asp Arg Val Glu Ser Leu Glu Leu Gly Cys Asp Tyr Tyr He

130 135 140

Gly Val Tyr Pro Glu Thr Leu Ala Ser Glu Phe His His Pro He Leu 145 150 155 160

Pro Leu Tyr Arg Val Asn Ala Phe Glu Ser Arg Asp Arg Glu Val Leu

165 170 175

Gin Val Leu Thr Ala He Lys Glu Asn Leu Pro Leu Arg Glu Val Pro

180 185 190

Leu Arg Ser Arg Gin Asp Val Phe He Ser Ala Ser Ser Leu Glu Lys

195 200 205

Leu Phe Gin Glu Arg Phe Pro Ala Ser Phe Gly Gin Phe Arg Lys Ala

210 215 220

Tyr Phe Arg His Phe Leu Arg Leu Gly Tyr 225 230

(2) INFORMATION FOR SEQ ID NO: 177:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 130 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 177:

Val Val Glu Arg He Lys He Ala Arg Ser Tyr Gly Asp Leu Ser Glu

1 5 10 15

Asn Ser Glu Tyr Glu Ala Ala Lys Asp Glu Gin Ala Phe Val Glu Gly

20 25 30

Gin He Ser Ser Leu Glu Thr Lys He Arg Tyr Ala Glu He Val Asn

35 40 45

Ser Asp Ala Val Ala Gin Asp Glu Val Ala He Gly Lys Thr Val Thr

50 55 60

He Gin Glu He Gly Glu Asp Glu Glu Glu Val Tyr He He Val Gly

261

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Ser Ala Gly Ala Asp Ala Phe Ala Gly Lys Val Ser Asn Glu Ser Pro

85 90 95

He Gly Gin Ala Leu He Gly Lys Lys Thr Gly Asp Thr Ala Thr He

100 105 110

Glu Thr Pro Val Gly Ser Tyr Asp Val Lys He Leu Lys Val Glu Lys

115 120 125

Thr Ala 130

(2) INFORMATION FOR SEQ ID NO: 178:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 79 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 178:

Val Asp Phe He Gly Gly Leu Ser Ala Leu Glu Gin Lys Gly Tyr Gin

1 5 10 15

Lys Gly Asp Glu He Leu He Asn Ser He Pro Arg Ala Leu Thr Glu

20 25 30

Thr Asp Lys Val Cys Ser Ser Val Asn He Gly Ser Thr Lys Ser Gly

35 40 45

He Asn Met Thr Ala Val Ala Asp Met Gly Arg He Tyr Gin Gly Asn

50 55 60

Gly Lys Ser Phe Arg Tyr Gly Ser Gly Gin Val Gly Cys He Arg 65 70 75

(2) INFORMATION FOR SEQ ID NO: 179:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 130 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

262

UBSTTTUTE SHEET (RULE 26) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 179:

Val Val Thr Pro Ala Asn Tyr Asn Thr Pro Ala Gin He Val He Ala

1 5 10 15

Gly Glu Val Val Ala Val Asp Arg Ala Val Glu Leu Leu Gin Glu Ala

20 25 30

Gly Ala Lys Arg Leu He Pro Leu Lys Val Ser Gly Pro Phe His Thr

35 40 45

Ala Leu Leu Glu Pro Ala Ser Gin Lys Leu Ala Glu Thr Leu Ala Gin

50 55 60

Val Ser Phe Ser Asp Phe Thr Cys Pro Leu Val Gly Asn Thr Glu Ala 65 70 75 80

Ala Val Met Gin Lys Glu Asp He Ala Gin Leu Leu Thr Arg Gin Val

85 90 95

Lys Glu Pro Val Arg Phe Tyr Glu Ser He Gly Val Met Gin Glu Ala

100 105 110

Gly He Ser Asn Phe He Arg Asp Trp Thr Gly Glu Ser Leu Val Arg

115 120 125

Phe Cys 130

(2) INFORMATION FOR SEQ ID NO: 180:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 29 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 180:

Val His Pro Thr Gly Pro Thr Pro Ala Thr Glu Thr Val Asp Ser He

1 5 10 15

Pro Gly Phe Glu Ala Pro Gin Glu Ser Val Thr He Leu 20 25

(2) INFORMATION FOR SEQ ID NO: 181:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 104 amino acids^"

(B) TYPE: amino acid

263

UBSTTTUTE SHEET (RULE 26) (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 181:

Val Pro Thr Val Phe His Lys Ser Ala Gin Val Leu Glu Glu Glu Met

1 5 10 15

Asn Arg Tyr Gin Pro Asp Phe Val Leu Cys He Gly Gin Ala Gly Gly

20 25 30

Arg Thr Ser Leu Thr Pro Glu Arg Val Ala He Asn Gin Asp Asp Ala

35 40 45

Arg Thr Ser Asp Asn Glu Asp Asn Gin Pro He Asp Arg Pro He Arg

50 55 60

Pro Asp Gly Ala Ser Ala Tyr Phe Ser Ser Leu Pro He Lys Ala Met 65 70 75 80

Val Gin Ala He Lys Lys Lys Asp Tyr Arg Pro Leu Phe Pro He Arg

85 90 95

Gin Gly Leu Leu Ser Ala Ala He 100

(2) INFORMATION FOR SEQ ID NO: 182:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 128 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 182:

Val Leu Gin Val Gly Ser Gin Asp Tyr Val Phe Val Leu Gin Gin Asp

1 5 10 15

Lys Tyr Thr Ser Val Arg Asp He Leu Ser Asp Thr He Glu Ala Val

20 25 30

Glu Tyr Asp Phe Gly Leu Arg Leu Ser He Met Leu Gly Gin Val Trp

35 40 45

Ser Gin Thr Gly His Gin Ala Leu Ser Asp Leu He Lys Ala Glu Arg

50 55 60

Asp Leu Phe Lys Thr Trp Trp Arg Gin Gly His Gin Gly Val His Thr

264

SUBSTTTUTE SHEET (RULE 26) 65 70 75 80

Phe Ser Gin Leu Tyr Leu Trp Ser Leu Gly Glu Arg Leu Val Asp Leu

85 90 95

Lys Pro He Lys Glu Cys Leu His Gin Met He Leu Asp Gin Asp Gin

100 105 110

He Gin Glu He He Leu Ser Leu Trp Glu Asn Ser Ala Val Leu Thr 115 120 125

(2) INFORMATION FOR SEQ ID NO: 183:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 214 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:183:

Val Arg Arg Ser Asp Arg Tyr Ala Arg Glu Val Gly Ala Asp Cys Val

1 5 10 15

Gly Glu Phe Val Ser Ala Thr Lys Thr Tyr Pro Val Ser Phe He Asn

20 25 30

Tyr Lys Gly Glu Glu Val Cys Leu Asp Gin Ala Pro Ala Gly Ser Ala

35 40 45

Pro Ala Ala Gin Phe Met Asp Gly Leu He Gly Tyr Gly Val Glu Gin

50 55 60

Leu He Ser Thr Gly Thr Cys Gly Val Leu Ala Asp He Glu Glu Asn 65 70 75 80

Ala Phe Leu Val Pro Val Arg Ala Leu Arg Asp Glu Gly Ala Ser Tyr

85 90 95

His Tyr Val Ala Pro Cys Arg Tyr Met Glu Met Gin Pro Glu Ala He

100 105 110

Ala Ala He Glu Glu Val Leu Glu Asp Arg Gly He Pro Tyr Glu Glu

115 120 125

Val Met Thr Trp Thr Thr Asp Gly Phe Tyr Arg Glu Thr Ala Glu Lys

130 135 140

Val Ala Tyr Arg Lys Glu Glu Gly Cys Ala Val Val Glu Met Glu Cys 145 150 155 160

Ser Ala Leu Ala Ala Val Ala Gin Leu Arg Gly Val Leu Trp Gly Glu

165 170 175

Leu Leu Phe Thr Ala Asn Ser Leu Ala Asp Leu Asp Gin Tyr Asn Ser

265

SUBSTTTUTE SHEET (RULE 26) 180 185 190

Arg Asp Trp Gly Ser Glu Pro Phe Asn Lys Ala Leu Lys Leu Ser Leu

195 200 205

Ala Ser Val His His Leu 210

(2) INFORMATION FOR SEQ ID NO : 184:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 136 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 184:

Val Glu Asn Leu Thr Asn Phe Tyr Glu Lys Tyr Arg Val Tyr Leu Thr

1 5 10 15

Arg Pro Arg Leu Glu Leu Leu Ala Val Val Thr He Val Leu Xaa Ala

20 25 30

Val Leu Val Phe Phe Leu Asn He Pro Gly Lys Gly Val Leu Lys Leu

35 40 45

Asp Asn Gly Thr He Val Tyr Asp Gly Ser Leu Val Arg Gly Lys Met

50 55 60

Asn Gly Gin Gly Thr He Thr Phe Gin Asn Gly Asp Gin Tyr Thr Gly 65 70 75 80

Gly Phe Asn Asn Gly Ala Phe Asn Gly Lys Gly Thr Phe Gin Ser Lys

85 90 95

Glu Gly Trp Thr Tyr Glu Gly Asp Phe Val Asn Gly Gin Ala Glu Gly

100 105 110

Lys Gly Lys Leu Thr Thr Glu Gin Glu Val Val Tyr Glu Gly Thr Phe

115 120 125

Lys Gin Gly Val Phe Gin Gin Lys 130 135

(2) INFORMATION FOR SEQ ID NO: 185:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 53 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

266

SUBSTTIUTΈ SHEET (RULE 26) (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: None

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 185:

Val Phe Leu Lys Glu Ser Cys Gly Ser Gly Ala Gin He Ala Glu Thr

1 5 10 15

Phe His Gin Phe Gly Gly Asp Tyr Gly Phe Glu Thr Thr Asp Leu Asn

20 25 30

Phe Asn Phe Ala Thr Leu Arg Arg Asn Arg Glu Ala Tyr He Asp Arg

35 40 45

Ala Arg Ser Ser Leu 50

267

SUBSTTTUTE SHEET (RULE 26)

Claims

What is claimed is

1. An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:

(a) a polynucleotide having at least a 70% identity to a polynucleotide encoding a polypeptide comprising an amino acid sequence of Table 1 ;

(b) a polynucleotide having at least a 70% identity to a polynucleotide encoding a mature polypeptide expressed by the gene contained in the S. pneumoniae of the deposited strain that was sequenced to obtain a polynucleotide sequence of Table 1 ;

(c) a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 70% identical to an amino acid sequence of Table 1 ;

(d) a polynucleotide which is complementary to the polynucleotide of (a), (b) or (c); and

(e) a polynucleotide comprising at least 15 sequential bases of the polynucleotide of (a), (b), (c) or (d).

2. The polynucleotide of Claim 1 wherein the polynucleotide is DNA.

3. The polynucleotide of Claim 1 wherein the polynucleotide is RNA.

4. The polynucleotide of Claim 2 comprising the nucleic acid sequence selected from the group consisting of the nucleic acid sequences set forth in Table 1.

5. The polynucleotide of Claim 2 which encodes a polypeptide comprising an amino acid sequence sequence selected from the group consisting of the amino acid sequences set forth in Table 1.

6. A vector comprising the polynucleotide of Claim 1.

7. A host cell comprising the vector of Claim 6.

8. A process for producing a polypeptide comprising: expressing from the host cell of Claim 7 a polypeptide encoded by said DNA.

9. A process for producing a polypeptide or fragment comprising culturing a host of claim 7 under conditions sufficient for the production of said polypeptide or fragment.

10. A polypeptide comprising an amino acid sequence which is at least 70% identical to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in Table 1.

11. A polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequences set forth in Table 1.

12. An antibody against the polypeptide of claim 10.

13. An antagonist or agonist of the activity or expression of the polypeptide of claim 10.

14. A method for the treatment or prevention of disease of an individual comprising: administering to the individual a therapeutically effective amount of the polypeptide of claim 10.

15. A method for the treatment of an individual having need to inhibit a bacterial polypeptide comprising: administering to the individual a therapeutically effective amount of the antagonist of Claim 13.

16. A process for diagnosing a disease related to expression or activity of the polypeptide of claim 10 in an individual comprising:

(a) determining a nucleic acid sequence encoding said polypeptide, and/or

(b) analyzing for the presence or amount of said polypeptide in a sample derived from the individual.

17. A method for identifying compounds which interact with and inhibit or activate an activity of the polypeptide of claim 10 comprising: contacting a composition comprising the polypeptide with the compound to be screened under conditions to permit interaction between the compound and the polypeptide to assess the interaction of a compound, such interaction being associated with a second component capable of providing a detectable signal in response to the interaction of the polypeptide with the compound; and determining whether the compound interacts with and activates or inhibits an activity of the polypeptide by detecting the presence or absence of a signal generated from the interaction of the compound with the polypeptide.

18. A method for inducing an immunological response in a mammal which comprises inoculating the mammal with the polypeptide of claim 10, or a fragment or variant thereof, adequate to produce antibody and/or T cell immune response to protect said animal from disease.

19. A method of inducing immunological response in a mammal which comprises delivering a nucleic acid vector to direct expression of a polypeptide of claim 10, or fragment or a variant thereof, for expressing said polypeptide, or a fragment or a variant thereof in vivo in order to induce an immunological response to produce antibody and/ or T cell immune response to protect said animal from disease.

269

SUBSTTTUTE SHEET (RULE 26)

20. A polynucleotide comprising a polynucleotide sequence selected from the group consisting of the the first ten polynucleotides sequences from the top of Table 1.

21. A polypeptide comprising a polypeptide encoded by the polynculeotide of claim 20.

22. The isolated polynucleotide of claim 1 wherein said nucleotide is selected from the group consisting of:

(a) a polynucleotide having at least a 90% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of Table 1 ;

(b) a polynucleotide having at least a 90% identity to a polynucleotide encoding the same mature polypeptide expressed by the gene contained in the S. pneumoniae of the deposited strain that was sequenced to obtain a polynucleotide sequence of Table 1 ;

(c) a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 90% identical to the amino acid sequence of Table 1 ;

(d) a polynucleotide which is complementary to the polynucleotide of (a), (b) or (c); and

(e) a polynucleotide comprising at least 15 sequential bases of the polynucleotide of (a), (b), (c) or (d).

23. The isolated polynucleotide of claim 1 selected from the group consisting of:

(a) a polynucleotide having at least a 95% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of Table 1 ;

(b) a polynucleotide having at least a 95% identity to a polynucleotide encoding the same mature polypeptide expressed by the gene contained in the S. pneumoniae of the deposited strain that was sequenced to obtain a polynucleotide sequence of Table 1 ;

(c) a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of Table 1;

(d) a polynucleotide which is complementary to the polynucleotide of (a), (b) or (c); and

(e) a polynucleotide comprising at least 15 sequential bases of the polynucleotide of (a), (b), (c) or (d).

24. An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:

270

SUBSTTTUTE SHEET (RULE 26) (a) a polynucleotide having at least a 50% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of Table 1 and obtained from a prokaryotic species other than S. pneumoniae;

(b) a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 50% identical to the amino acid sequence of Table 1 and obtained from a prokaryotic species other than S. pneumoniae; and

(c) a polynucleotide which is complementary to the polynucleotide of (a) or (b).

25. An isolated Streptococcal polypeptide having one of the amino acid sequences given in Table 1.

26. An isolated nucleic acid encoding one of the amino acid sequences of Claim 1 and nucleic acid sequences capable of hybridizing therewith under stringent conditions.

27. Recombinant vectors comprising the nucleic acid sequences of Claim 26 and host cells transformed or transfected therewith.

28. A method of identifying an antimicrobial compound comprising contacting candidate compounds with a polypeptide of Claim 1 and selecting those compounds capable of inhibiting the bioactivity of said polypeptide.

29. Antimicrobial compounds identified by the method of Claim 28.

30. An isolated Streptococcal polypeptide having one of the amino acid sequences given in Table 1.

31. An isolated nucleic acid encoding one of the amino acid sequences of Claim 30 and nucleic acid sequences capable of hybridizing therewith under stringent conditions.

32. Recombinant vectors comprising the nucleic acid sequences of Claim 31 and host cells transformed or transfected therewith.

33. A method of identifying an antimicrobial compound comprising contacting candidate compounds with a polypeptide of Claim 30 and selecting those compounds capable of inhibiting the bioactivity of said polypeptide.

34. Antimicrobial compounds identified by the method of Claim 33.

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SUBSTTTUTE SHEET (RULE 26)