WO1995030150B1 - Method for detecting free igfbp-1 - Google Patents
Method for detecting free igfbp-1Info
- Publication number
- WO1995030150B1 WO1995030150B1 PCT/IB1995/000364 IB9500364W WO9530150B1 WO 1995030150 B1 WO1995030150 B1 WO 1995030150B1 IB 9500364 W IB9500364 W IB 9500364W WO 9530150 B1 WO9530150 B1 WO 9530150B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- igfbp
- igf
- free
- monoclonal antibody
- detecting
- Prior art date
Links
- 210000002219 Extraembryonic Membranes Anatomy 0.000 claims abstract 3
- 239000007788 liquid Substances 0.000 claims abstract 2
- 102000005614 monoclonal antibodies Human genes 0.000 claims 11
- 108010045030 monoclonal antibodies Proteins 0.000 claims 11
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 8
- 102000004877 Insulin Human genes 0.000 claims 4
- 108090001061 Insulin Proteins 0.000 claims 4
- 239000003102 growth factor Substances 0.000 claims 4
- 210000004381 Amniotic Fluid Anatomy 0.000 claims 3
- 102000004965 antibodies Human genes 0.000 claims 3
- 108090001123 antibodies Proteins 0.000 claims 3
- 229960000070 antineoplastic Monoclonal antibodies Drugs 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 230000002608 insulinlike Effects 0.000 claims 2
- 229960000060 monoclonal antibodies Drugs 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 239000000020 Nitrocellulose Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000001809 detectable Effects 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 239000000975 dye Substances 0.000 claims 1
- 239000003365 glass fiber Substances 0.000 claims 1
- 230000005012 migration Effects 0.000 claims 1
- 229920001220 nitrocellulos Polymers 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 230000001681 protective Effects 0.000 claims 1
- 230000002285 radioactive Effects 0.000 claims 1
- 102000004375 Insulin-Like Growth Factor Binding Protein 1 Human genes 0.000 abstract 6
- 108090000957 Insulin-Like Growth Factor Binding Protein 1 Proteins 0.000 abstract 6
- 102400000022 Insulin-Like Growth Factor II Human genes 0.000 abstract 2
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 abstract 2
- 102000004218 Insulin-like growth factor I Human genes 0.000 abstract 2
- 108090000723 Insulin-like growth factor I Proteins 0.000 abstract 2
- 210000002966 Serum Anatomy 0.000 abstract 2
- 210000001691 Amnion Anatomy 0.000 abstract 1
- 102000013275 Somatomedins Human genes 0.000 abstract 1
- 108010026080 Somatomedins Proteins 0.000 abstract 1
Abstract
A method and device is described for detecting fetal membrane rupture by detecting only that insulin-like growth-factor-binding protein 1 (IGFBP-1) which is free of insulin-like growth factors 1 and 2 (IGF-1 and IGF-2). The method is based on the fact that the concentration of free IGFBP-1 in the blood serum is about ten times lower than that of the IGFBP-1 bound to IGF-1 and IGF-2, while the amniotic liquid which is contained in the amnion has more than 1000 times the concentration of free IGFBP-1 than the blood serum. The device comprises a one pad (22) and two strips (24, 26) test kit which may be used to detect free IGFBP-1 in a range of concentrations from 5 ng/ml to 250 νg/ml.
Claims
1. A method for detecting free insulin-like growth-fac¬ tor-binding protein 1 (IGFBP-1) in a sample containing amniotic fluid, where the fluid contains IGFBP-1 free of insulin growth factor 1 (IGF-1) and insulin growth factor 2 (IGF-2) , said method comprising: contacting the sample with a first labeled monoclonal antibody which is reactive with free IGFBP-1, to form an IGFBP-1- labeled antibody complex in the presence of free IGFBP-1 in the sample, reacting such complex with a solid support having immobilized thereon, a second monoclonal antibody which is reactive with free IGFBP-1, where said first and second monoclonal antibodies (i) bind to first and second different binding sites on IGFBP-1, respectively, and (ii) each competes with IGF-1 and IGF-2 for binding to IGFBP-1; and detecting the presence of IGFBP-1 in the sample by detecting the IGFBP-1-labeled antibody complex bound to the solid support.
2. The method of claim 1, where the first monoclonal antibody is attached to a detectable agent selected from the group consisting of insoluble particles, enzymes, dyes, and radioactive isotopes.
3. The method of claim 1, where said detecting is effective for diagnosing fetal membrane rupture.
4. The method of claim 3, where said detecting is effective to determine low levels of free IGFBP-1 present in the amniotic fluid at concentrations down to about 10 ng/ml.
5. A device for detecting free insulin-like growth-fac¬ tor-binding protein 1 (IGFBP-1) in a sample containing amniotic fluid, where the fluid contains IGFBP-1 free of insulin growth factor 1 (IGF-1) and insulin growth factor 2 (IGF-2) , said device comprising: 2
a first porous medium containing a first monoclonal antibody, said antibody being labeled, and a second porous medium composed of a first element and a second element, said first and second elements being intercon¬ nected to the first medium, and where said first and second media allow migration of a liquid sample therethrough, said first element having a test region containing a second monoclonal antibody fixed therein, said second element having a control region composed of (i) a first part containing the second monoclonal antibody fixed therein and (ii) a second part containing free IGFBP-1 fixed therein, where said first and second monoclonal antibodies bind to first and second different binding sites on IGFBP-1, respec¬ tively, and each monoclonal antibody competes with IGF-1 and IGF-2 for binding to IGFBP-1.
6. The device of claim 5, for use in the diagnosis of fetal membrane rupture.
7. The device of claim 5, where said first labeled monoclonal antibody is freeze-dried.
8. The device of claim 5, which further includes a protective film covering at least a portion of said device.
9. The device of claim 5, wherein said second porous medium further contains the first monoclonal antibody in non-labeled form.
10. The device of claim 5, where the first medium is composed of a pad of glass fibers and the second medium is composed of nitrocellulose.
11. The device of claim 8, in which said film contains an aperture for sample introduction in the region covering said first porous medium.
12. The device of claim 11, further including a casing enclosing said device, where said casing possesses an opening above said aperture and observation windows located above said test region and said control region.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU24163/95A AU706284B2 (en) | 1994-04-28 | 1995-04-27 | Method and device for detecting free IGFBP-1 |
| EP95918108A EP0755515B1 (en) | 1994-04-28 | 1995-04-27 | Method and device for detecting free igfbp-1 |
| JP07528104A JP2001501723A (en) | 1994-04-28 | 1995-04-27 | Method and apparatus for detecting free IGFBP-1 |
| DE69513160T DE69513160D1 (en) | 1994-04-28 | 1995-04-27 | METHOD AND TEST DEVICE FOR DETECTING FREE IGFBP-1 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/234,851 | 1994-04-28 | ||
| US08/234,851 US5597700A (en) | 1994-04-28 | 1994-04-28 | Method for detecting free insulin-like growth-factor-binding protein 1 and a test device for detecting the ruptures of fetal membranes using the above method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1995030150A1 WO1995030150A1 (en) | 1995-11-09 |
| WO1995030150B1 true WO1995030150B1 (en) | 1995-12-07 |
Family
ID=22883079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IB1995/000364 WO1995030150A1 (en) | 1994-04-28 | 1995-04-27 | Method for detecting free igfbp-1 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US5597700A (en) |
| EP (1) | EP0755515B1 (en) |
| JP (1) | JP2001501723A (en) |
| AT (1) | ATE186403T1 (en) |
| AU (1) | AU706284B2 (en) |
| CA (1) | CA2189050A1 (en) |
| DE (1) | DE69513160D1 (en) |
| WO (1) | WO1995030150A1 (en) |
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| EP1622942B1 (en) * | 2003-05-01 | 2014-11-19 | ImClone LLC | Fully human antibodies directed against the human insulin-like growth factor-1 receptor |
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| US11555816B2 (en) | 2010-09-01 | 2023-01-17 | Clinical Innovations, Llc | Detection of amniotic fluid in vaginal secretions of pregnant women due to premature rupture of fetal membranes |
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| CN107727841B (en) | 2013-01-02 | 2021-05-25 | 凯杰科学有限责任公司 | Method for predicting childbirth time of pregnant woman |
| US20160207988A1 (en) * | 2013-09-24 | 2016-07-21 | The Board Of Regents Of The University Of Texas System | A novel hormone that promotes bone resorption and uses thereof |
| KR20160140186A (en) * | 2015-05-29 | 2016-12-07 | 삼성전자주식회사 | Strip for analysis, apparaturs and system using the strip for analysis |
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| US10935555B2 (en) | 2016-12-22 | 2021-03-02 | Qiagen Sciences, Llc | Determining candidate for induction of labor |
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| EP0733205B1 (en) * | 1993-12-20 | 2001-09-26 | Subburaman Mohan | Method for removal of binding proteins from ligands |
| US5712103A (en) * | 1995-02-13 | 1998-01-27 | Regents Of The University Of California | Diagnostic assay for the prediction of preeclampsia |
-
1994
- 1994-04-28 US US08/234,851 patent/US5597700A/en not_active Expired - Fee Related
-
1995
- 1995-04-27 DE DE69513160T patent/DE69513160D1/en not_active Expired - Lifetime
- 1995-04-27 AU AU24163/95A patent/AU706284B2/en not_active Ceased
- 1995-04-27 CA CA002189050A patent/CA2189050A1/en not_active Abandoned
- 1995-04-27 EP EP95918108A patent/EP0755515B1/en not_active Revoked
- 1995-04-27 WO PCT/IB1995/000364 patent/WO1995030150A1/en not_active Application Discontinuation
- 1995-04-27 AT AT95918108T patent/ATE186403T1/en not_active IP Right Cessation
- 1995-04-27 JP JP07528104A patent/JP2001501723A/en active Pending
-
1996
- 1996-10-28 US US08/738,248 patent/US5877029A/en not_active Expired - Fee Related
- 1996-10-28 US US08/738,246 patent/US5891722A/en not_active Expired - Fee Related
- 1996-10-28 US US08/740,104 patent/US5968758A/en not_active Expired - Fee Related
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