TH15599C3 - Real-time multicolor fluorescent signal reading system - Google Patents

Abstract

------ 16/03/2018 ------ (OCR) page 1 of number 1 page invention summary. The invention involves a real-time multicolor fluorescent signal reading system consisting of a measurement unit. Control unit Display unit And processing unit Where the examination sheet For loading in the aforementioned examination sheets Was provided to show signs of substance Fluorescent, specific group fluorescent And the light system consists of a light source, a color camera lens system. And filter For bending unused light from a light source, where such light source It has one wavelength corresponding to the absorption spectrum of the phosphor. Fluorescent light, such specific groups This makes it possible to simultaneously receive multi-colored fluorescent images without the need to change the light source and the light filter. ------------ This invention relates to the signal reading system. Real-time multi-color fluorescent with the ability to simultaneously read multi-color fluorescent images on the microstructure. However, in one measurement point There may be more than one fluorescent color, so the light it emits is mixed. This type of measurement Will be used to detect gene expression The specialty of this invention is the simple measurement unit. No mechanical system To measure light, each color does not need to be changed. Light source And light filters The real-time multicolor fluorescent reading system consists of a measurement unit, a control unit. Display unit And processing unit Where the exam sheet is to be inserted in the loading area The signaling component is a specific group of fluorescent fluorescent and the optical system consists of a light source, a color camera lens system. And filter For cutting unused light from light sources Where the light source has a single wavelength corresponding to the fluorescent absorption spectrum, the specific group of fluorescent fluorescence allows for unnecessary simultaneous reception of multicolored fluorescent images. Need to change Light sources and filters In addition to the aforementioned processing units There is an image processing from both the fluor. A single resent or a maximum of three fluorescent mixtures at the same measurement location is achieved by using a mathematical computation process to calibrate the color scale in RGB color space (RGB) from An image obtained with the concentration of each fluorescent agent. In which this processing method Due to the mixture of fluorescent fluorescent substances Will be limited to the selection of phosphors A fluorescent light emitting spectrum that can be detected only by the red sensor or the green sensor. Or the blue sensor of the camera to receive any image That do not interfere with each other and at the same time measurements

Claims (9)

Disclaimers (all) which will not appear on the advertisement page: Edit 22/03/2019 Page 1 of 3 pages. 1. Real-time multicolor fluorescent signal reading system Containing Measurement unit (1) where it relies on an optical technique to read a fluorescent light image signal. St from the micro-array plate By unit of measure It consists of light system (2), power supply system (4) and check plate load section (3), control unit. Where it receives data from the measurement unit (1) and for entering data for use in the processing of the processing unit Display unit Where it serves to display the results obtained from the measurement unit (1) and the processing unit. Where is the function of processing the data obtained from the measuring unit (1) and the control and display units thereof A unique feature is the test plate (39) for loading in the load area. The test plate (3) is provided with a fluorescent indicator, a specific group of fluorescent and the optical system (2). Light source, color reading camera lens system And filter For cutting unused light from light sources Where such light source Wavelength This particular group of fluorescent fluorescent agents allows simultaneous reception of multicolored fluorescent images without the need for replacement. Light sources and filters In addition to the aforementioned processing units There is an image processing from both substances. One fluorescent or a combination of up to three fluorescents at the same measurement location. By using the mathematical calculation process to calibrate the values The color level in the RGB color space (RGB) from the image obtained with the concentration of each fluorescent agent, herein the processing method due to the mixing of the phosphor. Fluorescent It is limited to the selection of fluorescent fluorescent agents that are emitting light in the spectrum that can Can only be measured with a red sensor Or green sensor Or the blue sensor of the camera (5), one of the That do not interfere with each other's measurements And only at the same time -------------------------------------------------- -------------------------------------------------- ------ 16/03/2018 ------ (OCR) Page 1 of 3 pages. 1. Real-time multicolor fluorescent signal reading system Containing Measurement unit (1) where it relies on an optical technique to read a fluorescent light image signal. St from micro-array disc by measurement unit It consists of light system (2), power supply system (4) and check plate load section (3), control unit. Where it receives data from the measurement unit (1) and for entering data for use in the processing of the processing unit Display unit Where it serves to display the results obtained from the measurement unit (1) and the processing unit. Where is the function of processing the data obtained from the measuring unit (1) and the control and display units thereof Specifically, the test strips (37) are placed in the load section, the test strips (3) are provided for the presence of a specific fluorescent agent, specific fluorescence and optical system (2). It consists of a light source, a color camera lens system, and a light filter. For bending unused light from a light source. Where such light source Wavelength This particular group of fluorescent fluorescent agents allows simultaneous reception of multicolored fluorescent images without the need for replacement. Light sources and filters In addition to the aforementioned processing units There is an image processing from both substances. One fluorescent or a combination of up to three fluorescents at the same measurement location. By using the mathematical calculation process to calibrate the values The color level in the RGB color space (RGB) from the image obtained with the concentration of each fluorescent agent, herein the processing method due to the mixing of the phosphor. Fluorescent It is limited to the selection of fluorescent fluorescent agents that are emitting light in the spectrum that can Can only be measured with a red sensor Or green sensor Or the blue sensor of the camera (5), one of the That do not interfere with each other's measurements And only at the same time Real-time multicolor fluorescent signal reading system According to claim 1, where the phosphor Fluorescent light, such specific groups Selectable from the phosphors in the organic group (Luminescence Organic Compound) or fluorescent agent in the quantum dot group (Quantum dot). Or two of them together on page 2 of 3 pages 3. Real-time multicolor fluorescent signal reading system According to claim 2, where the phosphor Fluorescent light, such specific groups It has an absorption spectral value at a wavelength in the range of 300 nm to 400 nm and can emit light in the visible light spectrum. 4. Real-time multicolor fluorescent signal reading system According to claims 1 to 3, any one where the said processor Processing methods include image acquisition, image positioning to measure non-dimensional signals, noise suppression, and image signal processing for the determination of fluorescent content due to Mixing Or not mixtures of fluorescent substances 5. Real-time multicolor fluorescent signal reading system According to claims 1 to 4, any one where the said processor Compose color images by graphing a calibration equation (52) showing the relationship between the fluorescence concentration of each color and the intensity of the system-readable fluorescence. The sample measurement point is then read. Which contains a bi-colored fluorescent phosphor With reference to the intensity of light emitting Each fluorescent color readable And display the concentration of the sample at the measuring point from the calibration equation of each color 6. Real-time multicolor fluorescent signal reading system According to claims 1 to 5, any one where the said processor Calculate the concentration of the sample at each measuring point from the calibration equation for each color. With the fluorescent intensity of each point Together with the graph obtained from the calibration (curve fitting) with a linear equation 7. Real-time reading of multicolored fluorescent signals in accordance with any one of the claims 1 to 6 where the processing unit There is a mathematical process that is used to create a calibration equation, i.e. statistical calculation in Least square or Least Absolute Residual or Bisquare. The one 8. Real-time multicolor fluorescent signal reading system According to claims 1 to 7, any one where the said processor There is a mathematical calculation process that is used to calculate the curve suitable for the measured data (fitting curve) for graphing the calibration equation is a numerical calculation process. In the form of Singular Value Decomposition or SVD or Givens process or Householder process, or Householder process, or LU Decomposition), or a Cholesky process, or a single-factor separation (SVD) process for Rank Deficient H (Rank Deficient H). 9. Real-time multicolor fluorescent signal reading system According to the claim 1 to 8, any one of them contains additional memory 1 0. Real-time multi-color fluorescent reading system according to one of the claims 1 to 9, where the said control and display units. Can be assembled in the same kit ------------ 1. Real-time multicolor fluorescent signal reading system Containing Measurement unit (1) where it relies on an optical technique to read a fluorescent light image signal. St from the micro-array plate By unit of measure It consists of light system (2), power supply system (4) and check plate load section (3), control unit. Where it receives data from the measurement unit (1) and for entering data for use in the processing of the processing unit Display unit Where it serves to display the results obtained from the measurement unit (1) and the processing unit. Where is the function of processing the data obtained from the measuring unit (1) and the control and display units thereof Specifically, the test strips (37) are placed in the load area. These test strips (3) are equipped with a fluorescent indicator, a specific group of fluorescence, and a optical system (2). Light source, color reading camera lens system And filter For cutting unused light from light sources Where such light source Wavelength Only one that corresponds to the absorption spectrum of specific fluorescent fluorescent agents. Makes receiving multi-colored fluorescent images simultaneously Compatible without the need to change the origin Light and filter Gulp, both for the said processing unit There is an image processing from both substances. The fluorescence is small or a mixture of up to three fluorescents at the same measurement location, by using a mathematical calculation process to calibrate the color scale in the RGB color space ( RGB) from the image obtained with the concentration of each fluorescent agent, herein the processing method due to the mixing of the fluorescent fluorescent agent is limited to the selection. Using a fluorescent agent with light emitting spectrum that can be measured. Just a red sensor Or green sensor Or the blue sensor of the camera (5), one of them that does not interfere with each other. And only at the same time 2. Real-time multi-color fluorescent signal reading system According to claim 1, where the phosphor Fluorescent, such specific groups Selectable from the phosphors in the organic group (Luminescence Organic Compound) or fluorescent agent in the quantum dot group (Quantum dot). Or two of them together 3. Real-time multicolor fluorescent signal reading system According to claim 2, where the phosphor Fluorescent, such specific groups It has an absorption spectral value at wavelengths in the range of 300 nm to 400 nm and can emit light in the visible light spectrum. 4. Real-time multicolor fluorescent signal reading system According to any one of the 1 to 3 claims Where the processing unit Processing methods include image acquisition, image positioning, automatic signal measurement, noise reduction. And processing Video signal for determining the amount of fluorescent substances due to mixing or without Mixed with fluorescent substances 5. Real-time multicolor fluorescent signal reading system According to any one of the 1 to 4 claims Where the processing unit The color image was processed by graphing the calibration equation (52) showing the relationship between the phosphor concentration of each color. And the light intensity of the Emits fluorescent light that the system can read. The sample measurement point is then read. Which consists of Fluorescent paint, both colors With reference to the intensity of the fluorescent light emitting, but Each color readable And display the concentration of the sample at the measuring point from the calibration equation of each color 6. Real-time multicolor fluorescent signal reading system According to any one of the 1 to 5 claims Where the processing unit Calculate the concentration of the sample at each measuring point. From the calibration equation for each color With the fluorescence intensity of each point in conjunction with the curve obtained from the calibration (curve fitting) with a linear equation 7. Real-time multicolor fluorescent signal reading system According to any 1 to 6 claim Where the processing unit There is a mathematical calculation process. The method used to create the calibration equation is either the Least Square or Least Absolute Residual or Bisquare statistical calculation. 8. Real-time multicolor fluorescent signal reading system According to any 1 to 7 claim Where the processing unit There is a mathematical calculation process. That is used to calculate the curves Suitable for measuring data (fitting curve) for graphing, calibration equation is a Singular Value Decomposition (SVD) or Givens or Householder or LU Decomposition or Cholesky or SVD calculation process for Rank Deficient H. 9. Real-time multicolor fluorescent signal reading system According to any one of the 1 to 8 claims Supplemented with memory 1 0. Real-time multi-color fluorescent signal reading system According to any 1 to 9 claim Where the said control and display units Can be assembled in the same equipment