SG10201807208RA - Viral resistant cells and uses thereof - Google Patents

Viral resistant cells and uses thereof

Info

Publication number
SG10201807208RA
SG10201807208RA SG10201807208RA SG10201807208RA SG10201807208RA SG 10201807208R A SG10201807208R A SG 10201807208RA SG 10201807208R A SG10201807208R A SG 10201807208RA SG 10201807208R A SG10201807208R A SG 10201807208RA SG 10201807208R A SG10201807208R A SG 10201807208RA
Authority
SG
Singapore
Prior art keywords
viral
resistant cells
cell lines
viral resistant
resistant
Prior art date
Application number
SG10201807208RA
Inventor
Nan Lin
Joaquina Mascarenhas
Audrey Chang
David Onions
Henry George
Kevin Kayser
Original Assignee
Sigma Aldrich Co Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co Llc filed Critical Sigma Aldrich Co Llc
Publication of SG10201807208RA publication Critical patent/SG10201807208RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01144—Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (2.4.1.144)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01155—Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase (2.4.1.155)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14311—Parvovirus, e.g. minute virus of mice

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

VIRAL RESISTANT CELLS AND USES THEREOF The present invention provides mammalian cell lines that have been genetically engineered causing such cell lines to be resistant to viral entry and/or propagation, and provides methods of using said cell lines to reduce or prevent viral contamination of biologic production systems. Fig.1
SG10201807208RA 2014-03-04 2015-03-03 Viral resistant cells and uses thereof SG10201807208RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461947860P 2014-03-04 2014-03-04

Publications (1)

Publication Number Publication Date
SG10201807208RA true SG10201807208RA (en) 2018-09-27

Family

ID=54055790

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201807208RA SG10201807208RA (en) 2014-03-04 2015-03-03 Viral resistant cells and uses thereof
SG11201607038TA SG11201607038TA (en) 2014-03-04 2015-03-03 Viral resistant cells and uses thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201607038TA SG11201607038TA (en) 2014-03-04 2015-03-03 Viral resistant cells and uses thereof

Country Status (10)

Country Link
US (1) US10377990B2 (en)
EP (2) EP3736325A1 (en)
JP (2) JP6751347B2 (en)
KR (2) KR102354633B1 (en)
CN (2) CN111849921A (en)
BR (1) BR112016020287B1 (en)
ES (1) ES2797050T3 (en)
IL (2) IL247460B (en)
SG (2) SG10201807208RA (en)
WO (1) WO2015134488A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2797050T3 (en) 2014-03-04 2020-12-01 Sigma Aldrich Co Llc Resistant Viral Cells and Their Uses
WO2015196150A2 (en) 2014-06-20 2015-12-23 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
DK3218005T3 (en) 2014-11-12 2023-03-27 Seagen Inc GLYCAN INTERACTING COMPOUNDS AND METHODS OF USE
US10889845B2 (en) 2015-07-16 2021-01-12 University Of Copenhagen Production of N-glycoproteins for enzyme assisted glycomodification
IL258768B2 (en) * 2015-11-12 2023-11-01 Siamab Therapeutics Inc Glycan-interacting compounds and methods of use
EP3205719A1 (en) * 2016-02-15 2017-08-16 CEVEC Pharmaceuticals GmbH Cell lines for producing recombinant glycoproteins with di-antennary n-glycans, methods using the same, and recombinant glycoproteins
WO2017194699A1 (en) * 2016-05-13 2017-11-16 University Of Copenhagen A cell-based array platform
US11390913B2 (en) 2016-09-22 2022-07-19 Sigma-Aldrich Co. Llc Single primer to dual primer amplicon switching
WO2018094143A1 (en) 2016-11-17 2018-05-24 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
WO2018140478A1 (en) * 2017-01-24 2018-08-02 Sigma-Aldrich Co. Llc Viral resistant cells and culture systems
KR20240044544A (en) 2017-03-03 2024-04-04 씨젠 인크. Glycan-interacting compounds and methods of use
EP3382014A1 (en) 2017-03-29 2018-10-03 CEVEC Pharmaceuticals GmbH Recombinant glycoproteins with reduced antennary fucosylation
US11709156B2 (en) 2017-09-18 2023-07-25 Waters Technologies Corporation Use of vapor deposition coated flow paths for improved analytical analysis
US11709155B2 (en) 2017-09-18 2023-07-25 Waters Technologies Corporation Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes
CN107937349B (en) * 2017-11-27 2021-01-15 中国检验检疫科学研究院 Cell line for stably expressing African swine fever virus P54 protein and preparation and application thereof
EP3717640A1 (en) 2017-11-28 2020-10-07 Danmarks Tekniske Universitet Glycosylation of proteins
TW202003839A (en) * 2018-03-29 2020-01-16 美商建南德克公司 Modulating lactogenic activity in mammalian cells
CN108504693A (en) * 2018-04-04 2018-09-07 首都医科大学附属北京朝阳医院 The O-type that T synthase genes structure is knocked out using Crispr technologies glycosylates abnormal colon carcinoma cell line
EP3921413A1 (en) 2019-02-08 2021-12-15 Wisconsin Alumni Research Foundation (WARF) Humanized cell line
CN110029129A (en) * 2019-03-06 2019-07-19 华南农业大学 A kind of construction method of MDCK stable cell strain that expressing 1 gene of chicken ST3GAL
CN111735801B (en) * 2019-03-25 2021-09-28 南京大学 Fluorescence analysis method based on HCR and cation exchange reaction of hydrogel
US11807872B2 (en) 2019-08-27 2023-11-07 Wisconsin Alumni Research Foundation (Warf) Recombinant influenza viruses with stabilized HA for replication in eggs
US11918936B2 (en) 2020-01-17 2024-03-05 Waters Technologies Corporation Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding
WO2022012663A1 (en) * 2020-07-17 2022-01-20 创观（苏州）生物科技有限公司 Virus insusceptible animal and construction method therefor
CN116440269B (en) * 2022-01-10 2024-02-09 华中农业大学 Application of SLC35A1 gene as target spot in prevention and treatment of porcine epidemic diarrhea

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
USRE45721E1 (en) 1994-08-20 2015-10-06 Gendaq, Ltd. Relating to binding proteins for recognition of DNA
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
ES2341926T3 (en) 1998-03-02 2010-06-29 Massachusetts Institute Of Technology POLYPROTEINS WITH ZINC FINGERS THAT HAVE IMPROVED LINKERS.
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
AU2005299296A1 (en) * 2004-10-27 2006-05-04 Vanderbilt University Mammalian genes involved in infection
WO2006076641A2 (en) * 2005-01-14 2006-07-20 Monosol Rx, Llc Methods and compositions for preventing and treating a disease related to glycan dysregulation
US8232448B2 (en) * 2005-06-08 2012-07-31 The Regents Of The University Of California Transgenic mouse with a homozygous mutation in the CMAH gene
CA2615532C (en) 2005-07-26 2016-06-28 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
US20110030072A1 (en) * 2008-12-04 2011-02-03 Sigma-Aldrich Co. Genome editing of immunodeficiency genes in animals
US20110016546A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Porcine genome editing with zinc finger nucleases
EP2451823A4 (en) * 2009-07-06 2013-07-03 Alnylam Pharmaceuticals Inc Compositions and methods for enhancing production of a biological product
CN102858985A (en) * 2009-07-24 2013-01-02 西格马-奥尔德里奇有限责任公司 Method for genome editing
WO2011103137A1 (en) * 2010-02-17 2011-08-25 Alnylam Pharmaceuticals, Inc. Cell-based methods and reagents
CA2817765A1 (en) * 2011-01-06 2012-07-12 The Johns Hopkins University Method of production of recombinant glycoproteins with increased circulatory half-life in mammalian cells
CN104245932A (en) * 2012-01-11 2014-12-24 西格马-奥尔德里奇有限责任公司 Production of recombinant proteins with simple glycoforms
ES2797050T3 (en) 2014-03-04 2020-12-01 Sigma Aldrich Co Llc Resistant Viral Cells and Their Uses

Also Published As

Publication number Publication date
JP2020202847A (en) 2020-12-24
CN111849921A (en) 2020-10-30
JP2017506903A (en) 2017-03-16
IL247460B (en) 2020-08-31
CN106661569B (en) 2020-09-11
CN106661569A (en) 2017-05-10
EP3114222A4 (en) 2017-08-02
IL247460A0 (en) 2016-11-30
BR112016020287B1 (en) 2022-06-28
KR20160127780A (en) 2016-11-04
US10377990B2 (en) 2019-08-13
ES2797050T3 (en) 2020-12-01
KR102354633B1 (en) 2022-01-24
SG11201607038TA (en) 2016-09-29
IL276679B (en) 2022-02-01
EP3736325A1 (en) 2020-11-11
JP6751347B2 (en) 2020-09-02
IL276679A (en) 2020-09-30
EP3114222A1 (en) 2017-01-11
US20160369240A1 (en) 2016-12-22
KR20220013460A (en) 2022-02-04
WO2015134488A1 (en) 2015-09-11
BR112016020287A2 (en) 2017-10-24
EP3114222B1 (en) 2020-04-22

Similar Documents

Publication Publication Date Title
SG10201807208RA (en) Viral resistant cells and uses thereof
NZ765695A (en) Immune cell organoid co-cultures
IL262772A (en) Genetically engineered cells and methods of making the same
MX2021008143A (en) Methods for harvesting mammalian cell cultures.
IL249828A0 (en) Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof
MX2020000621A (en) Methods and compositions for immunomodulation.
SG10201810150UA (en) Compositions and methods for enhanced gene expression in cone cells
IL248451B (en) Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy
PH12017500861A1 (en) Anti-interleukin-33 antibodies and uses thereof
EP3371314A4 (en) Genomic engineering of pluripotent cells
WO2016161169A3 (en) Cell culture incubators with integrated cell manipulation systems
MX2017005920A (en) Anti-tim3 antibodies and methods of use.
SG10201810723VA (en) Improved t cell compositions
MY188799A (en) Anti-cd79b antibodies and methods of use
PH12016501435A1 (en) Anti-jagged1 antibodies and methods of use
IL273405B1 (en) Rpe cell populations and methods of generating same
MX2017007196A (en) Immortalised chicken embryo fibroblasts.
MY182924A (en) Enzyme compositions and uses thereof
GB201602974D0 (en) Engineered cells & methods (1)
MX2016015893A (en) Production of fc fragments.
PH12018502061A1 (en) Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
EP4295914A3 (en) Genetically-engineered drug resistant t cells and methods of using the same
EP3472305A4 (en) Engineered treg cells
EP3436623A4 (en) Apparatuses and systems for vertical electrolysis cells
MX2022000516A (en) Methods relating to pluripotent cells.