SG10201804421XA - Column-based device and method for retrieval of rare cells based on size, and uses thereof - Google Patents
Column-based device and method for retrieval of rare cells based on size, and uses thereofInfo
- Publication number
- SG10201804421XA SG10201804421XA SG10201804421XA SG10201804421XA SG10201804421XA SG 10201804421X A SG10201804421X A SG 10201804421XA SG 10201804421X A SG10201804421X A SG 10201804421XA SG 10201804421X A SG10201804421X A SG 10201804421XA SG 10201804421X A SG10201804421X A SG 10201804421XA
- Authority
- SG
- Singapore
- Prior art keywords
- column
- retrieval
- size
- teccs
- expression
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 206010028980 Neoplasm Diseases 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 102100029761 Cadherin-5 Human genes 0.000 abstract 1
- 101000794587 Homo sapiens Cadherin-5 Proteins 0.000 abstract 1
- 101001116302 Homo sapiens Platelet endothelial cell adhesion molecule Proteins 0.000 abstract 1
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 abstract 1
- 101150045640 VWF gene Proteins 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000001772 blood platelet Anatomy 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 210000002889 endothelial cell Anatomy 0.000 abstract 1
- 230000003511 endothelial effect Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 210000000265 leukocyte Anatomy 0.000 abstract 1
- 210000003593 megakaryocyte Anatomy 0.000 abstract 1
- 238000004393 prognosis Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 102100036537 von Willebrand factor Human genes 0.000 abstract 1
Classifications
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5064—Endothelial cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
- B01D15/125—Pre-filtration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G01N33/57419—Specifically defined cancers of colon
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- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/12—Special parameters characterising the filtering material
- B01D2239/1216—Pore size
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
- B01D39/1692—Other shaped material, e.g. perforated or porous sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
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- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
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- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Ecology (AREA)
- Vascular Medicine (AREA)
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Abstract
COLUMNBASED DEVICE AND METHOD FOR RETRIEVAL OF RARE CELLS BASED ON SIZE, AND USES THEREOF A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor- 10 derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device. (Fig. 1) 92
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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SG10201500471Q | 2015-01-21 | ||
SG10201500472R | 2015-01-21 |
Publications (1)
Publication Number | Publication Date |
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SG10201804421XA true SG10201804421XA (en) | 2018-07-30 |
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ID=56417483
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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SG11201705625WA SG11201705625WA (en) | 2015-01-21 | 2016-01-21 | Column-based device and method for retrieval of rare cells based on size, and uses thereof |
SG10201804421XA SG10201804421XA (en) | 2015-01-21 | 2016-01-21 | Column-based device and method for retrieval of rare cells based on size, and uses thereof |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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SG11201705625WA SG11201705625WA (en) | 2015-01-21 | 2016-01-21 | Column-based device and method for retrieval of rare cells based on size, and uses thereof |
Country Status (9)
Country | Link |
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US (3) | US11084034B2 (en) |
EP (2) | EP3650096A3 (en) |
JP (2) | JP6693966B2 (en) |
KR (1) | KR102421024B1 (en) |
CN (2) | CN107430116B (en) |
AU (1) | AU2016209694B2 (en) |
CA (1) | CA2975726C (en) |
SG (2) | SG11201705625WA (en) |
WO (1) | WO2016118086A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP6693966B2 (en) | 2015-01-21 | 2020-05-13 | エージェンシー フォー サイエンス, テクノロジー アンド リサーチ | Column-based device and method for collecting rare cells based on size and use thereof |
EP4235179A3 (en) * | 2017-03-16 | 2023-11-15 | Université Libre de Bruxelles | Detection, quantification and/or isolation of circulating tumor cells based on the expression of cd321 marker |
US10372967B1 (en) * | 2018-07-18 | 2019-08-06 | Intervet Inc. | Automated parasite analysis system |
US20220360703A1 (en) * | 2021-05-06 | 2022-11-10 | The Regents Of The University Of California | Remote microscopy for biological sciences |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4915848A (en) | 1986-04-21 | 1990-04-10 | Miles Laboratories, Inc. | Red blood cell filtering system |
AU2003240795A1 (en) * | 2002-05-24 | 2003-12-12 | Invitrogen Corporation | Nested pcr employing degradable primers |
AU2004286307A1 (en) * | 2003-10-31 | 2005-05-12 | Vitatex, Inc. | Blood test prototypes and methods for the detection of circulating tumor and endothelial cells |
US7790039B2 (en) * | 2003-11-24 | 2010-09-07 | Northwest Biotherapeutics, Inc. | Tangential flow filtration devices and methods for stem cell enrichment |
CN1997431A (en) * | 2004-03-30 | 2007-07-11 | 蒂特根博士生物技术 | Filter with support cage |
DE602007008080D1 (en) | 2006-04-18 | 2010-09-09 | Wellstat Biologics Corp | DETECTION OF CIRCULATING ENDOTHELIAL CELLS |
CN200940146Y (en) * | 2006-08-18 | 2007-08-29 | 王茜 | Sieve like large intestine cells collector |
WO2008038785A1 (en) * | 2006-09-29 | 2008-04-03 | Toray Industries, Inc. | Cell-adsorbing column |
WO2009038754A2 (en) | 2007-09-19 | 2009-03-26 | The Research Foundation Of State University Of New York | Gene expression signatures in enriched tumor cell samples |
US20090191535A1 (en) * | 2007-12-22 | 2009-07-30 | Mark Carle Connelly | Method of assessing metastatic carcinomas from circulating endothelial cells and disseminated tumor cells |
FR2926091B1 (en) * | 2008-01-09 | 2014-09-05 | Metagenex | METHOD AND DEVICE FOR ISOLATING AND CULTIVATING LIVING CELLS ON A FILTER |
US20140315295A1 (en) | 2013-03-15 | 2014-10-23 | Creatv Microtech, Inc. | Polymer microfilters, devices comprising the same, methods of manufacturing the same, and uses thereof |
WO2011139233A1 (en) | 2010-05-04 | 2011-11-10 | Agency For Science, Technology And Research | A microsieve for cells and particles filtration |
JP2012044876A (en) * | 2010-08-24 | 2012-03-08 | Olympus Corp | Centrifugal container |
CN103748208A (en) * | 2011-07-01 | 2014-04-23 | 恰根有限公司 | Filter module in biomolecule isolation |
CN102304581B (en) | 2011-09-01 | 2013-10-30 | 广州好芝生物科技有限公司 | Kit and method for detecting KRAS genetic mutation |
EP2782653A4 (en) | 2011-11-21 | 2016-01-20 | Creatv Microtech Inc | Polymer microfiltration devices, methods of manufacturing the same and the uses of the microfiltration devices |
EP2695656A1 (en) * | 2012-08-09 | 2014-02-12 | F. Hoffmann-La Roche AG | Method and separation device for separating a filtrate from a sample liquid |
WO2015088979A1 (en) | 2013-12-09 | 2015-06-18 | The Scripps Research Institute | Methods for the detection and quantification of circulating endothelial cells |
CN203754717U (en) * | 2014-01-14 | 2014-08-06 | 刘韬 | Cell separation device adopting fluid film filtering |
WO2015116780A1 (en) | 2014-01-31 | 2015-08-06 | Epic Sciences, Inc. | Methods for the detection and quantification of circulating tumor cell mimics |
JP6693966B2 (en) | 2015-01-21 | 2020-05-13 | エージェンシー フォー サイエンス, テクノロジー アンド リサーチ | Column-based device and method for collecting rare cells based on size and use thereof |
CN218994908U (en) * | 2022-12-20 | 2023-05-09 | 杭州迪安生物技术有限公司 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
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2016
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JP2018504115A (en) | 2018-02-15 |
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KR20170104518A (en) | 2017-09-15 |
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CN112358943A (en) | 2021-02-12 |
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EP3650096A2 (en) | 2020-05-13 |
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US20180008982A1 (en) | 2018-01-11 |
KR102421024B1 (en) | 2022-07-14 |
US20220001385A1 (en) | 2022-01-06 |
WO2016118086A1 (en) | 2016-07-28 |
EP3248004A4 (en) | 2018-10-03 |
JP6693966B2 (en) | 2020-05-13 |
CN107430116A (en) | 2017-12-01 |
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