CN218994908U - Cell film-making of high filterability is with filtration membrane section of thick bamboo - Google Patents
Cell film-making of high filterability is with filtration membrane section of thick bamboo Download PDFInfo
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- CN218994908U CN218994908U CN202223427089.9U CN202223427089U CN218994908U CN 218994908 U CN218994908 U CN 218994908U CN 202223427089 U CN202223427089 U CN 202223427089U CN 218994908 U CN218994908 U CN 218994908U
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Abstract
The application relates to a high filterability cell is filtration membrane section of thick bamboo for film-making, this scheme includes: one end of the lower membrane fixing piece is provided with an inlet for the falling cells and the liquid culture medium to flow into the lower membrane fixing piece, the inner ring is provided with a plane with an outlet for the installation of the lower filter membrane, the plane with the outlet is provided with air holes, and the air holes are not blocked by the upper filter membrane; the lower layer filter membrane is arranged at the inner ring of the lower layer membrane fixing piece; the upper layer film fixing piece is provided with a plurality of through holes at one end facing the lower layer film fixing piece and is detachably connected with the lower layer film fixing piece; the upper layer filter membrane is arranged at one end of the upper layer membrane fixing piece facing the lower layer membrane fixing piece and covers all the through holes; and the filling piece is arranged between the upper membrane fixing piece and the upper filter membrane. The application can effectively adsorb the exfoliated cells and quantitatively and uniformly smear the exfoliated cells on a glass slide.
Description
Technical Field
The application relates to the technical field of cell tabletting, in particular to a high-filterability filtering membrane cylinder for cell tabletting.
Background
Typically, exfoliated cells are obtained from a sample of the human body (e.g., exfoliated cells and liquid medium) and then smeared (thinly spread) on a glass slide in order to accurately observe and diagnose the state of the exfoliated cells. Here, conventional smearing on slides for pap (media) testing is performed manually using a tool (e.g., a dropper, a cotton swab).
However, due to the nature of the shed cells, there is a risk of infection if the shed cells are exposed to the skin of the user. Furthermore, due to manual application, the exfoliated cells overlap each other to form an uneven layer, thereby interfering with observation and diagnosis. At present, although some technologies of filtering membrane cylinder structures, such as chinese patent No. CN210495944U and chinese patent No. CN112638534a, can solve the above problems to a certain extent, they do not have a good cell enrichment effect, which is not beneficial to subsequent rubbing operation.
Therefore, there is a need for a high filterability filter cartridge for cell slide making that allows a sample to efficiently adsorb exfoliated cells, and quantitatively and uniformly smear the exfoliated cells on a slide glass, and has an enrichment effect.
Disclosure of Invention
The present application aims to solve the above problems in the prior art and to provide a filter membrane cartridge for cell tabletting with high filterability.
In order to achieve the purpose of the application, the application adopts the following technical scheme: a high-filterability cell-tabletting filter membrane cartridge comprises:
one end of the lower membrane fixing piece is provided with an inlet for the falling cells and the liquid culture medium to flow into the lower membrane fixing piece, the inner ring is provided with a plane with an outlet for the installation of the lower filter membrane, the plane with the outlet is provided with air holes, and the air holes are not blocked by the upper filter membrane; a sample bottle for connecting the sample bottle with the exfoliated cells stored therein;
the lower layer filter membrane is arranged at the inner ring of the lower layer membrane fixing piece and is used for filtering impurities;
the upper layer film fixing piece is provided with a plurality of through holes at one end facing the lower layer film fixing piece and is detachably connected with the lower layer film fixing piece;
the upper layer filter membrane is arranged at one end of the upper layer membrane fixing piece facing the lower layer membrane fixing piece and covers all through holes and is used for quantitatively adsorbing the exfoliated cells and filtering the liquid culture medium;
and the filling piece is arranged between the upper membrane fixing piece and the upper filter membrane and is used for filling the upper filter membrane and providing elastic deformation force required during rubbing.
Working principle and beneficial effect: 1. compared with the prior art, the enrichment function is embodied between the upper filter membrane and the lower filter membrane. When the filter membrane cylinder is in an inverted state and negative pressure liquid suction is carried out, a large number of dropped cells in the liquid culture medium are enriched between the double-layer filter membrane structures (an upper-layer filter membrane and a lower-layer filter membrane) and attached to the upper-layer filter membrane, so that the next rubbing link is facilitated, namely, the cells are uniformly distributed on a special filter membrane (the upper-layer filter membrane) through the double-layer filter membrane structures (the upper-layer filter membrane and the lower-layer filter membrane) in cooperation with vacuum adsorption, and therefore, the cell ring cells which are finally rubbed on a glass slide are uniformly distributed; while avoiding direct contact with or exposure to the skin of the user;
2. compared with the prior art, the effect of the bleeder vent of this application is when bottom negative pressure drawing liquid, provides forward pressure. Ensuring that the liquid can smoothly pass through the filtering membrane to achieve the effect of enriching cells at a designated position;
3. compared with the prior art, the double-layer filter membrane structure (an upper-layer filter membrane and a lower-layer filter membrane) can filter out larger impurities, so that the interference condition on the last rubbing on a glass slide is avoided;
4. compared with the prior art, the method and the device can actually detect the cell concentration according to the external device, and quantitatively adsorb the cells on the glass slide by utilizing the external device to enable the cells on the glass slide to accord with the specified quantity range.
Further, the inner ring of the lower film fixing piece is provided with threads for connecting with a sample bottle. The lower layer film fixing piece can be conveniently arranged on the sample bottle, and the lower layer film fixing piece is convenient to assemble and disassemble and simple in structure.
Further, the inner ring part of the lower layer film fixing piece matched with the upper layer film fixing piece is provided with a plurality of convex edges which can be tightly matched with the outer ring at the bottom of the upper layer film fixing piece. Make the cooperation between lower floor's membrane mounting and the upper film mounting inseparabler through the bead, improve sealed effect.
Further, the upper film fixing piece is provided with a mounting groove for mounting the filling piece. And the filling piece is convenient to install.
Further, the matching part of the upper layer membrane fixing piece and the upper layer filter membrane is provided with an inclined step so as to fix the upper layer filter membrane. And the upper filter membrane is convenient to install.
Further, the top of the upper layer film fixing piece is provided with a first conical surface matched with the lower layer film fixing piece, and the bottom of the lower layer film fixing piece is provided with a second conical surface matched with the first conical surface. Can improve the sealing effect between upper film fixing piece and the lower film fixing piece, and is convenient to assemble and disassemble.
Further, the upper layer film fixing piece is provided with a stress structure for facilitating the disassembly and assembly of the upper layer film fixing piece and the lower layer film fixing piece.
Further, the filling member is a sponge.
Further, a circular flange is arranged on the plane of the outlet of the belt to form a groove for installing the lower filter membrane.
Further, the air holes are positioned on the inner ring of the circular flange.
Drawings
FIG. 1 is a perspective view of a filter membrane cartridge according to the present application.
FIG. 2 is an exploded view of the filter membrane cartridge of FIG. 1 according to the present application.
FIG. 3 is an exploded view of the filter membrane cartridge of FIG. 1 according to the present application.
FIG. 4 is a cross-sectional perspective view of a filter membrane cartridge.
Fig. 5 is a front view of fig. 4.
FIG. 6 is a perspective view of the filter membrane cartridge after inversion to begin adsorption.
FIG. 7 is a perspective view of the separation rubbing after the filter cartridge is inverted.
In the figure, 1, a lower film fixing piece; 2. a lower layer filter membrane; 3. an upper film fixing member; 4. an upper layer filter membrane; 5. a filling member; 11. a plane with an outlet; 12. ventilation holes; 13. a thread; 14. a rib; 15. a second conical surface; 16. a circular flange; 31. a mounting groove; 32. a first conical surface; 33. an inclined step; 34. and a stress structure.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application are within the scope of the protection of the present application.
It will be appreciated by those skilled in the art that in the disclosure of the present application, the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," etc. refer to an orientation or positional relationship based on that shown in the drawings, which is merely for convenience of description of the present application and to simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore the above terms should not be construed as limiting the present application.
In this example, a sample was obtained from exfoliated cells and a liquid medium, and the exfoliated cells were mixed in a cell preservation solution. In addition, the sample may contain byproducts obtained from the human body other than exfoliated cells or liquid medium.
As shown in fig. 1 to 7, the high-filterability filtering membrane cartridge for cell preparation is used for effectively adsorbing rubbing exfoliated cells, and comprises:
the lower membrane fixing piece 1 is hollow and smooth in the interior, one end is provided with an inlet for the falling cells and the liquid culture medium to flow into the lower membrane fixing piece 1, the other end is connected with the lower membrane fixing piece 1, the inner ring is provided with a plane 11 with an outlet for the installation of the lower filter membrane 2, the plane 11 with the outlet is provided with an air hole 12 (the air hole 12 is positioned at the inner ring of the circular flange 16), and the air hole 12 is not blocked by the upper filter membrane 4; a sample bottle for connecting the sample bottle with the exfoliated cells stored therein;
in this embodiment, the inner ring of the lower membrane fixing member 1 is provided with a thread 13 for connecting with a sample bottle, detachable connection is realized by screw-coupling with the sample bottle, and the inner ring portion of the lower membrane fixing member 1 and the upper membrane fixing member 3 is provided with a plurality of ribs 14, and the ribs 14 can be tightly fitted (interference fit) with the outer ring of the bottom of the upper membrane fixing member 3.
Preferably, for better mounting of the filling member 5, the upper film fixing member 3 is provided with a mounting groove 31 for mounting the filling member 5.
Preferably, the strip outlet plane 11 is provided with a circular flange 16 to form a groove for the installation of the lower filter membrane 2.
The lower layer filter membrane 2 is arranged at the inner ring (the middle inlet step) of the lower layer membrane fixing piece 1 and is used for filtering larger impurities; wherein the lower filter membrane 2 is preferably a nylon mesh.
The upper layer film fixing piece 3 is provided with a plurality of through holes towards one end of the lower layer film fixing piece 1 and is detachably connected with the lower layer film fixing piece 1, so that the upper layer film fixing piece 3 is separated from the lower layer film fixing piece 1 after being absorbed and is combined with the lower layer film fixing piece 1 after rubbing;
in this embodiment, in order to facilitate installation of the upper filter membrane 4, the portion of the upper membrane fixing member 3 that mates with the upper filter membrane 4 is provided with an inclined step 33 to fix the upper filter membrane 4.
Preferably, in order to improve the sealing effect, the top of the upper film fixing member 3 is provided with a first conical surface 32 matched with the lower film fixing member 1, and the bottom of the lower film fixing member 1 is provided with a second conical surface 15 matched with the first conical surface 32. Can maintain good sealing performance under the action of external force.
Preferably, the stress structure 34 is used for facilitating the disassembly and assembly of the upper layer film fixing member 3 and the lower layer film fixing member 1 for convenience. Such as the flaring ring in this embodiment.
The upper layer filter membrane 4 is arranged at one end of the upper layer membrane fixing piece 3 facing the lower layer membrane fixing piece 1 and covers all through holes, and is used for quantitatively adsorbing the exfoliated cells and filtering the liquid culture medium; wherein the upper filter membrane 4 is preferably a nuclear pore membrane, also known as a track etched membrane.
And the filling piece 5 is arranged between the upper membrane fixing piece 3 and the upper filter membrane 4 and is used for filling the upper filter membrane 4, so that the upper filter membrane 4 can be fully attached to a glass slide during rubbing, and the sponge is fixed on the upper end surface of the upper membrane fixing piece 3 and is attached to the lower part of the upper filter membrane 4. Wherein the filling member 5 may be a sponge or a material of similar physical properties. Wherein the filling member 5 is preferably a compressed sponge. The function is mainly to provide the elastic deformation required by the back surface during rubbing, which is more beneficial to cell transfer.
In this embodiment, the lower membrane holder 1 is not separated from the upper membrane holder 3 when the filter membrane cartridge device is used upside down. In this inverted state, the exfoliated cells or the liquid medium flows in through the inlet of the lower membrane holder 1. The lower filter membrane 2 blocks the passage of larger byproducts outside of the shed cells or liquid medium.
In addition, referring to fig. 6, the upper filter membrane 4 and sponge block the passage of shed cells or liquid medium or smaller byproducts. When the falling-off cell processing device (prior art, not the object to be protected in the present application) applies negative pressure to the filter membrane cartridge of the present application, the falling-off cells are uniformly attached to the upper layer filter membrane 4 under the action of external force, and the liquid culture medium is discharged through the upper layer filter membrane 4 and the sponge (filling member 5).
As shown in fig. 3, the filter membrane cartridge is in an inverted state, and the ventilation holes 12 are used to balance the air pressure inside the lower membrane holder 1 during the adsorption process.
As shown in FIG. 7, after the filtration membrane cartridge is inverted to complete the adsorption, the detached cell processing apparatus (not shown) is in a detachably fastened state with the upper membrane holder 3. The falling-off cell processing device moves downwards to drive the upper layer film fixing piece 3 to be separated from the lower layer film fixing piece 1.
In addition, the upper membrane filter 4 is located above the lower membrane holder 1. When the upper filter membrane 4 is contacted with the glass slide, the detached cells can be rubbed onto the glass slide.
As shown in fig. 6, after rubbing is completed, the exfoliated cell treatment device moves up with the upper membrane fixing member 3 and is in a detachable fastening state with the lower membrane fixing member 1. The external force acts on the outer ring, and the exfoliated cell treating device is separated from the upper membrane fixing member 3.
As shown in fig. 5, after the second inversion, the filtering membrane cartridge can be subjected to subsequent treatment.
The detailed description of the present application is not prior art, and thus is not described in detail herein.
It will be understood that the terms "a" and "an" should be interpreted as referring to "at least one" or "one or more," i.e., in one embodiment, the number of elements may be one, while in another embodiment, the number of elements may be plural, and the term "a" should not be interpreted as limiting the number.
Although terms of the lower membrane holder 1, the lower filter membrane 2, the upper membrane holder 3, the upper filter membrane 4, the filling member 5, the band-out plane 11, the ventilation holes 12, the threads 13, the ribs 14, the second tapered surface 15, the circular rib 16, the mounting groove 31, the first tapered surface 32, the inclined step 33, the force receiving structure 34, etc. are used more herein, the possibility of using other terms is not excluded. These terms are used merely for convenience in describing and explaining the essence of the present application; they are to be interpreted as any additional limitation that is not inconsistent with the spirit of the present application.
The present application is not limited to the above-mentioned preferred embodiments, and any person can obtain other products in various forms under the teaching of the present application, but any changes in shape or structure of the products are within the scope of protection of the present application.
Claims (10)
1. A high filterability cell-making filtration membrane cartridge comprising:
an inlet for the falling cells and the liquid culture medium to flow into the lower membrane fixing piece (1) is formed in one end of the lower membrane fixing piece, a plane (11) with an outlet for the lower membrane (2) to be installed is formed in the inner ring of the lower membrane fixing piece, ventilation holes (12) are formed in the plane (11) with the outlet, and the ventilation holes (12) are not blocked by the upper membrane (4); a sample bottle for connecting the sample bottle with the exfoliated cells stored therein;
the lower layer filter membrane (2) is arranged at the inner ring of the lower layer membrane fixing piece (1) and is used for filtering impurities;
the upper layer film fixing piece (3) is provided with a plurality of through holes towards one end of the lower layer film fixing piece (1) and is detachably connected with the lower layer film fixing piece (1);
the upper layer filter membrane (4) is arranged at one end of the upper layer membrane fixing piece (3) facing the lower layer membrane fixing piece (1) and covers all through holes, and is used for quantitatively adsorbing the exfoliated cells and filtering the liquid culture medium;
and the filling piece (5) is arranged between the upper membrane fixing piece (3) and the upper filter membrane (4) and is used for filling the upper filter membrane (4) and providing elastic deformation force required during rubbing.
2. A highly filterable filter cartridge for cell tableting according to claim 1, wherein the inner collar of the lower membrane holder (1) is provided with a screw thread (13) for connecting the sample bottle.
3. A highly filterable filter cartridge for cell tableting according to claim 1, wherein the inner ring portion of the lower membrane holder (1) and the upper membrane holder (3) is provided with a plurality of ribs (14), the ribs (14) being capable of being tightly fitted to the outer ring of the bottom of the upper membrane holder (3).
4. A highly filterable filter cartridge for cell tableting according to claim 1, wherein the upper membrane fixing member (3) is provided with a mounting groove (31) for mounting the filling member (5).
5. A high filterability cell-tabletting filter cartridge according to claim 1, wherein the upper membrane fixing member (3) is provided with an inclined step (33) at the position where it cooperates with the upper filter membrane (4) to fix the upper filter membrane (4).
6. The high-filterability filtering membrane cartridge for cell tabletting according to claim 1, wherein a first conical surface (32) matched with the lower membrane fixing member (1) is arranged at the top of the upper membrane fixing member (3), and a second conical surface (15) matched with the first conical surface (32) is arranged at the bottom of the lower membrane fixing member (1).
7. The high-filterability filtering membrane cartridge for cell tabletting according to claim 1, wherein the upper membrane fixing member (3) is provided with a stress structure (34) for facilitating the disassembly and assembly of the upper membrane fixing member (3) and the lower membrane fixing member (1).
8. A high filterability cell tableting filter membrane cartridge according to any one of claims 1 to 7, wherein the filling member (5) is a sponge.
9. A high filterability cell-tabletting filter cartridge according to any one of claims 1 to 7, wherein the strip outlet plane (11) is provided with a circular flange (16) to form a channel for mounting the lower filter (2).
10. A highly filterable filter cartridge for cell tableting according to claim 9, wherein the ventilation holes (12) are located in the inner periphery of the circular rim (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223427089.9U CN218994908U (en) | 2022-12-20 | 2022-12-20 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223427089.9U CN218994908U (en) | 2022-12-20 | 2022-12-20 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
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| CN218994908U true CN218994908U (en) | 2023-05-09 |
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| CN202223427089.9U Active CN218994908U (en) | 2022-12-20 | 2022-12-20 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358943A (en) * | 2015-01-21 | 2021-02-12 | 新加坡科技研究局 | Column-based apparatus and method for size-based recovery of rare cells, and uses thereof |
| CN115876551A (en) * | 2022-12-20 | 2023-03-31 | 杭州迪安生物技术有限公司 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
-
2022
- 2022-12-20 CN CN202223427089.9U patent/CN218994908U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358943A (en) * | 2015-01-21 | 2021-02-12 | 新加坡科技研究局 | Column-based apparatus and method for size-based recovery of rare cells, and uses thereof |
| CN115876551A (en) * | 2022-12-20 | 2023-03-31 | 杭州迪安生物技术有限公司 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
| CN115876551B (en) * | 2022-12-20 | 2023-08-11 | 杭州迪安生物技术有限公司 | Cell film-making of high filterability is with filtration membrane section of thick bamboo |
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