SE9901339D0 - New expression systems - Google Patents
New expression systemsInfo
- Publication number
- SE9901339D0 SE9901339D0 SE9901339A SE9901339A SE9901339D0 SE 9901339 D0 SE9901339 D0 SE 9901339D0 SE 9901339 A SE9901339 A SE 9901339A SE 9901339 A SE9901339 A SE 9901339A SE 9901339 D0 SE9901339 D0 SE 9901339D0
- Authority
- SE
- Sweden
- Prior art keywords
- expression
- intron
- gene
- present
- expression systems
- Prior art date
Links
Abstract
The present invention relates to expression systems for the expression of recombinant DNA in mammalian cells using introns in the expression constructs. According to the present invention intron IV of the human NPY receptor Y1 gene has been found to increase considerably the expression of a number of proteins previously able to be expressed only at very low levels. The present invention provides an expression system of a human NPY receptor gene, comprising e.g. intron IV of the human NPY receptor Y1 gene, or functional variants of the said intron. The invention also comprises of producing proteins at high levels. The invention also relates to a method of screening compounds for NPY agonist or antagonist activity.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9901339A SE9901339D0 (en) | 1999-04-15 | 1999-04-15 | New expression systems |
PCT/SE2000/000714 WO2000063404A1 (en) | 1999-04-15 | 2000-04-14 | New expression systems using introns |
AU43235/00A AU4323500A (en) | 1999-04-15 | 2000-04-14 | New expression systems using introns |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9901339A SE9901339D0 (en) | 1999-04-15 | 1999-04-15 | New expression systems |
Publications (1)
Publication Number | Publication Date |
---|---|
SE9901339D0 true SE9901339D0 (en) | 1999-04-15 |
Family
ID=20415221
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE9901339A SE9901339D0 (en) | 1999-04-15 | 1999-04-15 | New expression systems |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU4323500A (en) |
SE (1) | SE9901339D0 (en) |
WO (1) | WO2000063404A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9657290B2 (en) | 2012-07-03 | 2017-05-23 | The Board Of Trustees Of The Leland Stanford Junior University | Scalable bio-element analysis |
US10370653B2 (en) | 2015-02-22 | 2019-08-06 | The Board Of Trustees Of The Leland Stanford Junior University | Micro-screening apparatus, process, and products |
WO2018089953A1 (en) | 2016-11-14 | 2018-05-17 | Orca Biosystems, Inc. | Methods and apparatuses for sorting target particles |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3545126A1 (en) * | 1985-12-19 | 1987-06-25 | Boehringer Mannheim Gmbh | METHOD FOR IMPROVING THE EXPRESSION OF EUKARYON PROTEIN |
WO1993004171A1 (en) * | 1991-08-19 | 1993-03-04 | Symbicom Aktiebolag | Human beta-casein, process for producing it and use thereof |
US5571695A (en) * | 1991-11-06 | 1996-11-05 | Garvan Institute Of Medical Research | Human neuropeptide Y-Y1 receptor |
US5965392A (en) * | 1996-04-08 | 1999-10-12 | Bayer Corporation | Neuropeptide Y receptor Y5 and nucleic acid sequences |
DK0846770T3 (en) * | 1996-06-12 | 2006-07-10 | Japan Tobacco Inc | Method for expression of foreign genes and vectors thereof |
-
1999
- 1999-04-15 SE SE9901339A patent/SE9901339D0/en unknown
-
2000
- 2000-04-14 AU AU43235/00A patent/AU4323500A/en not_active Abandoned
- 2000-04-14 WO PCT/SE2000/000714 patent/WO2000063404A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
AU4323500A (en) | 2000-11-02 |
WO2000063404A1 (en) | 2000-10-26 |
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