SE9901339D0 - New expression systems - Google Patents

New expression systems

Info

Publication number
SE9901339D0
SE9901339D0 SE9901339A SE9901339A SE9901339D0 SE 9901339 D0 SE9901339 D0 SE 9901339D0 SE 9901339 A SE9901339 A SE 9901339A SE 9901339 A SE9901339 A SE 9901339A SE 9901339 D0 SE9901339 D0 SE 9901339D0
Authority
SE
Sweden
Prior art keywords
expression
intron
gene
present
expression systems
Prior art date
Application number
SE9901339A
Other languages
Swedish (sv)
Inventor
Mona Bystroem
Jonas Ekstrand
Ulrica Marklund
Original Assignee
Astra Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astra Ab filed Critical Astra Ab
Priority to SE9901339A priority Critical patent/SE9901339D0/en
Publication of SE9901339D0 publication Critical patent/SE9901339D0/en
Priority to PCT/SE2000/000714 priority patent/WO2000063404A1/en
Priority to AU43235/00A priority patent/AU4323500A/en

Links

Abstract

The present invention relates to expression systems for the expression of recombinant DNA in mammalian cells using introns in the expression constructs. According to the present invention intron IV of the human NPY receptor Y1 gene has been found to increase considerably the expression of a number of proteins previously able to be expressed only at very low levels. The present invention provides an expression system of a human NPY receptor gene, comprising e.g. intron IV of the human NPY receptor Y1 gene, or functional variants of the said intron. The invention also comprises of producing proteins at high levels. The invention also relates to a method of screening compounds for NPY agonist or antagonist activity.
SE9901339A 1999-04-15 1999-04-15 New expression systems SE9901339D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE9901339A SE9901339D0 (en) 1999-04-15 1999-04-15 New expression systems
PCT/SE2000/000714 WO2000063404A1 (en) 1999-04-15 2000-04-14 New expression systems using introns
AU43235/00A AU4323500A (en) 1999-04-15 2000-04-14 New expression systems using introns

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9901339A SE9901339D0 (en) 1999-04-15 1999-04-15 New expression systems

Publications (1)

Publication Number Publication Date
SE9901339D0 true SE9901339D0 (en) 1999-04-15

Family

ID=20415221

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9901339A SE9901339D0 (en) 1999-04-15 1999-04-15 New expression systems

Country Status (3)

Country Link
AU (1) AU4323500A (en)
SE (1) SE9901339D0 (en)
WO (1) WO2000063404A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9657290B2 (en) 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
US10370653B2 (en) 2015-02-22 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
WO2018089953A1 (en) 2016-11-14 2018-05-17 Orca Biosystems, Inc. Methods and apparatuses for sorting target particles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3545126A1 (en) * 1985-12-19 1987-06-25 Boehringer Mannheim Gmbh METHOD FOR IMPROVING THE EXPRESSION OF EUKARYON PROTEIN
WO1993004171A1 (en) * 1991-08-19 1993-03-04 Symbicom Aktiebolag Human beta-casein, process for producing it and use thereof
US5571695A (en) * 1991-11-06 1996-11-05 Garvan Institute Of Medical Research Human neuropeptide Y-Y1 receptor
US5965392A (en) * 1996-04-08 1999-10-12 Bayer Corporation Neuropeptide Y receptor Y5 and nucleic acid sequences
DK0846770T3 (en) * 1996-06-12 2006-07-10 Japan Tobacco Inc Method for expression of foreign genes and vectors thereof

Also Published As

Publication number Publication date
AU4323500A (en) 2000-11-02
WO2000063404A1 (en) 2000-10-26

Similar Documents

Publication Publication Date Title
BRPI0516572A (en) methods and compositions for improving the production of recombinant proteins
NZ514951A (en) Recombinant protein production in a cultured human cell
MXPA05007781A (en) Fusion constructs and use of same to produce antibodies with increased fc receptor binding affinity and effector function.
WO2000060060A3 (en) Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
BR9813608B1 (en) transgenic bacillus host cell, and process for producing a polypeptide having aminopeptidase activity.
ATE323770T1 (en) POLYPEPTIDES WITH LYSOPHOSPHOLIPASE ACTIVITY AND NUCLEIC ACIDS THAT ENCOD THEM
DE69939327D1 (en) FUNCTIONAL ANTAGONISTS OF HEDGEHOG ACTIVITY
ATE450603T1 (en) POLYPEPTIDES WITH BRANCHING ENZYME ACTIVITY AND NUCLEIC ACIDS ENCODING THEM
WO2005016966A3 (en) Intron fusion proteins, and methods of identifying and using same
PT973794E (en) NETRINA RECEPTORS
WO2003016511A1 (en) Method of extended culture for antigen-specific cytotoxic t lumphocytes
BR0016886A (en) Trichoderma reesei xylanase
Langedijk et al. A structural model of pestivirus Erns based on disulfide bond connectivity and homology modeling reveals an extremely rare vicinal disulfide
TNSN05187A1 (en) Overexpression vector for animal cell
DK1157100T3 (en) Oxaloacetate hydrolase-defective fungal host cells
WO1997026322A3 (en) Methods and compositions for inhibiting hexokinase
SE9901339D0 (en) New expression systems
WO2000012678A3 (en) Staphylococcus aureus genes and polypeptides
AU7485996A (en) Neuropeptide y-y5 receptor
JP2016538840A (en) Recombinant glycoprotein and use thereof
WO2001000643A3 (en) Polynucleotides and polypeptides encoded thereby distantly homologous to heparanase
WO2003074652A3 (en) Methods for identifying agents that modulate mast cell degranulation
WO2000066735A3 (en) Human ion channel proteins
AU5166200A (en) Human tumor necrosis factor receptor tr10
Benkhaial et al. Effects of 5-fluorouracil on collagen synthesis during quail secondary palate development.