KR20120085504A - Diagnostic Equipment using magnetic resistance sensor - Google Patents

Abstract

PURPOSE: A diagnosing device using a magnetoresistance sensor is provided to rapidly and simply analyze even though the concentration of an object is low and to obtain a result with high sensitivity. CONSTITUTION: A diagnosing device(10) using a magnetoresistance sensor(200) comprises a holder(310) and an enhancer(320). The holder fixes a specimen accommodating unit(430). The enhancer comprises a magnetic substance for amplifying magnetic signals. The specimen accommodating unit comprises a detection area and accommodates a specimen(410) joined with magnetic particles.

Description

Diagnostic equipment using magnetic resistance sensor

The present invention relates to a diagnostic apparatus using a magnetoresistive sensor having an enhancer for amplifying an output signal.

A device for testing or examining the presence of a single or a plurality of substances in a liquid sample, such as a urine or blood sample, is called a diagnostic kit or measuring cartridge. Specifically, the modern diagnostic business is being integrated into one point-of-care testing (POCT). POCT is a test that takes place outside the centralized laboratory and is available to the general public without expertise. Currently, the diagnosis area is expanding from the hospital to the field and the individual.

Medical devices or measuring devices that perform a certain diagnosis using such a diagnostic kit include an electrochemical blood analyzer, an optical blood analyzer, and a magnetic field measuring device. An electrochemical blood analyzer includes voltage, current, and resistance from a measurement cartridge. It is driven by the principle of deriving the value and using it for the measurement, and the optical hematology analyzer is driven by acquiring an image of the test cartridge test line and measuring the pixel intensity of the acquired image.

1 is a conceptual diagram illustrating a principle of measuring or sensing a magnetoresistive sensor. However, for convenience of description, the sensing principle using a giant magneto resistance (GMR) of the magnetoresistive sensor will be described as an example. This shows a spin-valve type Giant Magneto Resistance (GMR) device. As shown, the magnetoresistive sensor has a nonmagnetic metal layer sandwiched between two ferromagnetic metal layers. The magnetic force of the ferromagnetic metal layer of the first layer is fixed, and the magnetic force of the ferromagnetic material of the second layer is variably adjusted. When the first layer and the magnetic force are parallel, the principle that only electrons spin-oriented in a specific direction passes through the conductor. That is, according to the alignment of the magnetization directions of the two ferromagnetic layers, a difference in electrical resistance, or a potential difference, induced inside the material is generated and recognized as a digital signal. The case where an interlayer material is a conductor corresponds to a GMR device. The diagnostic device using the magnetoresistive sensor is a high-sensitivity field inspection device capable of quantitatively measuring the magnetic particles accumulated in the lateral flow membrane using a giant magnetoresistive sensor.

However, in the case of a diagnostic device using such a magnetoresistive sensor, there is a problem that the measurement is not properly performed when the output signal of the sensor by the accumulated magnetic particles is smaller than the noise of the sensor itself or the basic noise of the diagnostic device circuit. Accordingly, when the sample is a low concentration sample, it is difficult to measure.

The present invention has been made to solve the above problems, and provides a diagnostic device coupled to the holder of the diagnostic device with an enhancer having a magnetic material for amplifying the magnetic signal, the amplified output when measuring the output signal of the sample with the diagnostic device By measuring the signal, the object is to enable quantitative measurement even when the sample is a low concentration sample.

Diagnostic apparatus using a magnetoresistive sensor of the present invention for solving the above problems, the holder for fixing the sample receiving portion; An enhancer having a magnetic material for amplifying a magnetic signal and coupled to the holder; It is configured to include, the sample receiving portion is provided with a detection area can accommodate the sample combined with the magnetic particles.

In the diagnostic device using a magnetoresistive sensor of the present invention, the enhancer includes: a magnetic signal amplification line formed by etching after depositing the magnetic material on a film to a predetermined thickness; . ≪ / RTI >

In addition, the magnetic material may include one or more of Fe, Mn, Ni, Co.

In addition, the enhancer may be attached to an upper side of the holder.

In addition, the enhancer may be attached to an upper surface of the holder, and may be formed such that the magnetic signal amplification line is positioned in an area corresponding to the detection area when the specimen receiving part is fixed to the holder.

In the diagnostic device using the magnetoresistive sensor of the present invention, the enhancer may include a magnetic signal amplification line formed by etching after depositing the magnetic material to a predetermined thickness on the upper surface of the holder.

In this case, the magnetic signal amplification line may be formed on an upper surface of the holder corresponding to the detection area when the specimen accommodating part is fixed to the holder.

In the diagnostic device using a magnetoresistive sensor of the present invention, the enhancer includes: a capillary tube having a tubular passage; Self-curing filler is added to the magnetic material is filled in the capillary tube; . ≪ / RTI >

In addition, the self-curing filler may include an ultraviolet curing filler to which the magnetic body is added.

In addition, the self-curing filler may include Poly (Ethylene Glycol) -Diacrylate (PEG-DA) and 2-Hydroxy-2-methyl-1-phenyl-propan-1-one.

In addition, the capillary tube may be formed in a structure in which both ends are sealed with a sealing material.

In addition, in the diagnostic device using a magnetoresistive sensor of the present invention, the magnetic body may be made of the same material as the magnetic particles.

In addition, in the diagnostic device using the magnetoresistive sensor of the present invention, the enhancer may be formed by being attached to the upper surface of the holder corresponding to the detection area, when the specimen receiving portion is fixed to the holder.

The holder may further include an enhancer insert formed on an upper surface of the holder and provided with a space for inserting the enhancer; Includes, the enhancer may be inserted into the enhancer inserting portion and coupled with the holder.

In this case, the enhancer inserting portion may be formed on an upper surface of the holder corresponding to the detection area when the specimen receiving portion is fixed to the holder.

A diagnostic device using a magnetoresistive sensor of the present invention, comprising: an external magnetic field applying device for applying an external magnetic field to the specimen; A magnetoresistive sensor for detecting a magnetic component of the sample according to the external magnetic field; As shown in FIG.

In this case, the external magnetic field applying device may include one or more of a solenoid coil, a Helmholtz coil, and an electromagnetic yoke.

The magnetoresistive sensor may also include at least one large magnetoresistive (GMR) device.

The present invention provides a diagnostic device in which an enhancer for amplifying a magnetic signal is combined with a holder of a diagnostic device, so that even if the concentration of the sample is low, quick and simple analysis required in the field diagnosis can be obtained and the sensitivity can be improved. It can be effective.

The present invention has the effect of providing a new diagnostic device capable of improving the sensitivity and quantitative analysis even when the noise of the sensor itself or the basic noise of the diagnostic device circuit occurs by amplifying the output signal of the sample.

According to the present invention, by combining an enhancer with a holder of a diagnostic device, it is possible to obtain a measurement result with improved sensitivity without complicated modification or modification of the diagnostic device.

In addition, the present invention is coupled to the enhancer to the holder of the diagnostic device, the position of the magnetic material of the enhancer is fixed does not change every time the output signal of the sample is measured has the effect of reducing the measurement error.

1 is a conceptual diagram illustrating a measuring principle of a magnetoresistive sensor.
2 is a conceptual diagram illustrating a principle of magnetic signal amplification of magnetic particles according to the present invention.
3 is a block diagram showing the configuration of a diagnostic device according to the present invention.
Figure 4 shows an embodiment of the external shape of the diagnostic device according to the present invention.
FIG. 5 illustrates an internal configuration of the diagnostic device of FIG. 3.
Figure 6 shows an embodiment in which the sample receiving portion is coupled to the holder of FIG.
7 to 9 show a holder according to each embodiment of the present invention.
FIG. 10 shows a method of manufacturing an enhancer in the form of a capillary tube of FIG. 9.
11 shows an example of the operation of measuring the output signal of the sample with the diagnostic device according to the present invention.
12 and 13 are graphs showing the output signal measurement value of the specimen with or without the enhancer of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, it is to be understood that the embodiments shown in the specification and the drawings shown in the drawings are only exemplary embodiments of the present invention, and that various equivalents and modifications may be substituted for them at the time of the present application. In addition, in describing the operating principle of the preferred embodiment of the present invention in detail, if it is determined that the detailed description of the related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted. The following terms are terms defined in consideration of functions in the present invention, and the meaning of each term should be interpreted based on the contents throughout the present specification. The same reference numerals are used for parts having similar functions and functions throughout the drawings.

2 is a conceptual diagram illustrating a principle of magnetic signal amplification of magnetic particles according to the present invention. Referring to FIG. 2, in general, when an external magnetic field is applied to the magnetic particles, a magnetic field is formed as in the dotted line of FIG. The magnetoresistive sensor measures the magnetic field formed by the magnetic particles, and in order to increase the sensitivity of the magnetoresistive sensor, the strength of the magnetic field formed by the magnetic particles must be increased.

As one of methods for increasing the strength of the magnetic field, the following method was used in the present invention. That is, as shown in (b) of FIG. 2, when a magnetic body forming a magnetic field is disposed around the magnetic particles, and a magnetic field is applied from the outside, the magnetic field generated from the magnetic particles and the magnetic field formed in the magnetic body are combined to form an overall magnetic field strength. It is amplified. As such, when a magnetic body capable of always forming the same magnetic field around the magnetic particles has an effect of amplifying the magnetic field formed in the magnetic particles, the sensitivity of the magnetoresistive sensor is improved. As a result, the sensitivity of the diagnostic device is improved. It is a way to improve. Accordingly, even when the sample is a low concentration sample and the amount of magnetic particles is distributed in the detection region, quantitative and accurate measurement is possible.

3 is a block diagram showing the configuration of a diagnostic device according to the present invention.

Referring to FIG. 3, the diagnostic device 10 according to the present invention includes a holder 310 for fixing a sample accommodating part 430, and an enhancer 320 provided with a magnetic material for amplifying a magnetic signal and coupled to the holder 310. It is configured to include. In addition, the diagnostic device 10 includes an external magnetic field applying device 100 for applying an external magnetic field to the sample 410, a magnetoresistive sensor 200 for detecting magnetic components of the sample 410 according to the external magnetic field, and a diagnostic device. It is preferable that the control unit 500 further includes an overall control.

Here, the sample accommodating part 430 is provided with a detection area to accommodate the sample 410 coupled with the magnetic particles. The sample accommodating part 430 may be a diagnostic kit (measurement cartridge) or a membrane, but is not limited thereto. The sample accommodating part 430 may include all accommodating parts capable of accommodating the sample 410.

The external magnetic field applying device 100 applies an external magnetic field to the sample 430 coupled with the magnetic particles to detect the magnetic signal for the sample 430 in the magnetoresistive sensor 200 so as to separate and analyze it as an electrical component. . The external magnetic field applying device 100 of the present invention may include at least one of a solenoid coil, a Helmholtz coil, and an electromagnetic yoke. Here, the magnetic particles may have a magnetization value of 10 to 100 emu / g, in which case the magnetic particles may have superparamagnetism or paramagnetism.

The magnetoresistive sensor 200 detects a magnetic component of the sample 430 to which the external magnetic field is applied by the external magnetic field applying device 100. The magnetoresistive sensor 200 of the present invention includes a normal magnetoresistance (OMR) sensor, anisotropic magnetoresistance (AMR) sensor, a giant magnetoresistance (GMR) sensor, and a colossal magnetoresistance (Colossal Magnetoresistance) , CMR) sensor, Tunneling Magnetoresistance (TMR) sensor, MJT (Magnetic Tunneling Junction) sensor, Planar Hall Resistance (Planar Hall Resistance) sensor is preferably used any one selected from. Particularly preferably, a giant magnetoresistance (GMR) sensor may be used.

Holder 310 may be formed in a structure for fixing the sample receiving portion 430, it is preferable that the sample receiving portion 430 is formed in a structure that can be removable by inserting or the like. However, the present invention is not limited thereto and should be interpreted as including all structures capable of fixing the sample accommodating part 430.

The enhancer 320 includes a magnetic body that forms a magnetic field when an external magnetic field is applied thereto, and is coupled to the holder 310. The shape of the enhancer 320 may have a film form, a metal thin film form, a capillary tube form, but is not limited thereto.

Meanwhile, the holder 310 in which the sample accommodating part 430 is fixed and the enhancer 320 is coupled needs to be transferred to an area of the magnetic field formed by the external magnetic field applying device 100 for more efficient measurement. A drive module 300 is required to perform horizontal movement of the holder 310 to the lower portion of the magnetoresistive sensor 200 or to perform shanghai movement so that the magnetoresistive sensor 200 can measure with high sensitivity after horizontal movement. Done. In this case, the diagnostic device 10 may be configured to further include a drive module 300, the holder 310 may be part of the drive module 300, but is not limited thereto.

The controller 500 may control the operation of the diagnostic apparatus 10 as a whole and may include a processor for performing such an operation. For example, the overall function of the diagnostic apparatus 10 may be controlled, such as the operation of the external magnetic field applying device 100, the operation of the driving module 300, and the analysis of the magnetic signal detected by the magnetoresistive sensor 200.

An embodiment of a diagnostic device according to the present invention having such a configuration will be described in more detail with reference to FIGS. 4 to 5.

Figure 4 shows an embodiment of the external shape of the diagnostic device according to the present invention. Referring to FIGS. 3 and 4, one end of the holder 310 may protrude to the outside to insert the sample accommodating part 430, for example, a diagnostic kit, and may display a result after completion of detection. D) and a printing unit P for printing the result may be further provided. It may also include a case portion (C) surrounding the outside of the diagnostic device.

The display unit D may include a liquid crystal display (LCD), a thin film transistor-liquid crystal display (TFT-LCD), a light emitting diode (LED), an organic light emitting diode (OLED), a flexible display, and the like. 3D display may include expression means such as liquid crystal. When such a representation means consists of a touch screen, it functions simultaneously as an input device and a representation device.

 In addition, the printing unit P may perform printing in any manner currently developed and commercialized, such as a dot matrix method, an inkjet method, a laser method, and a dye sublimation method, or may be implemented according to future technological developments.

FIG. 5 illustrates an internal configuration of the diagnostic device of FIG. 4.

3 to 5, an enhancer (not shown) is coupled to the holder 310. When the sample accommodating part 430 is inserted into the holder 310 to which the enhancer is coupled, the control part 500 horizontally holds the sample accommodating part 430 as an area in which a magnetic field is formed in the lower portion of the external magnetic field applying device 100. It can be moved (reciprocating in the R3 direction). This horizontal movement may be driven by the drive module 300, the drive module 300 of the X-axis drive motor 340 to the transfer unit 330, such as a belt to move the holder 310 horizontally By receiving the driving force (rotation in the R1 direction) to be transported to the lower side of the external magnetic field applying device (100). At this time, the holder 310 is moved along the guide rail 350.

When the holder 310 arrives in a space of an area formed under the external magnetic field applying device 100 by the driving of the driving module 300, the control unit 500 drives the driving module 300 to drive the magnetoresistive sensor 200. It drives the Z-axis drive motor 370 to move up and down the support unit 360 to move up and down (up and down in the R2 direction). Here, the magnetoresistive sensor 200 is attached to the end of the support unit 360.

The magnetoresistive sensor 200 descending by the support unit 360 is mounted on the holder 310 to stop on the fixed sample receiving portion 430 to measure the magnetic signal. In this case, the measured magnetic signal is a magnetic signal amplified by the enhancer, and thus, even when the sample 410 is a low concentration sample, more accurate measurement is possible.

On the other hand, for more accurate detection it is preferable to control the holder 310 through the control unit 500 to measure the maximum value of the electric signal that is changed by reciprocating in the same direction as the measurement direction of the magnetoresistive sensor 200. The maximum value of the electrical signal measured at this time is again amplified by the enhancer. Then, the measured magnetic signal of the sample 410 is sent to the control unit 500, and then the result value is derived through the display unit (D). In addition, it is possible to print the result value derived through the printing unit (P).

Figure 6 shows an embodiment in which the sample receiving portion is coupled to the holder of FIG.

5 and 6, as illustrated in FIG. 6A, the enhancer 320 may be formed on the upper surface of the holder 310. In this case, the enhancer 320 may be formed of any one of a film form, a metal thin film deposited directly on the upper surface of the holder 310, and a capillary tube form, but is not limited thereto. The magnetic material may be a magnetic material that generates a magnetic field with respect to an external magnetic field. If it does, there is no limit to the form. The magnetic material may include one or more of Fe, Ni, Mn, and Co. Preferably, the magnetic material may include Ni, but is not limited thereto. The magnetic material may include any material that forms a magnetic field with respect to an external magnetic field. Should be interpreted.

Thereafter, as shown in FIG. 6B, the sample accommodating part 430 is coupled to the holder 310, and the enhancer 320 is positioned below the detection area of the sample accommodating part 430. Thereafter, as shown in FIG. 6C, the holder 310 in which the sample accommodating part 430 is mounted has an inner direction (R3 direction) of the diagnostic device along the guide rail 350, for example, a lower portion of the external magnetic field applying device. The magnetic resistance of the sample is measured by the magnetoresistive sensor.

7 shows a holder according to a first embodiment of the present invention.

Referring to FIG. 7, in the present embodiment, the enhancer 321 may include a magnetic signal amplification line 323 formed by etching a magnetic material on a film to a predetermined thickness.

The magnetic material may include one or more of Fe, Ni, Mn, and Co. Preferably, the magnetic material may include Ni, but is not limited thereto. The magnetic material may include any material that forms a magnetic field with respect to an external magnetic field. Should be interpreted.

The film may be made of any material capable of depositing and etching magnetic materials, and the film itself is preferably made of a nonmagnetic material that does not form a magnetic field with respect to an external magnetic field. Meanwhile, the magnetic deposition process and the etching process may be made of a number of known processes, and detailed description thereof will be omitted.

As an example, when the magnetic material is Ni, Ni is deposited on a film formed of polyethylene terephthalate (PET), a pattern is printed on the film using a silk screen printing machine, and then the printed portion is subjected to an etching process in an etching furnace. Only Ni remains and the rest are removed. In this manner, the magnetic signal amplification line 323 included in the film-type enhancer 321 may be formed.

In addition, a method of forming the magnetic signal amplification line 323 by directly printing the material including the magnetic material on the film may be implemented through a process similar to the above-described method.

However, the above-described process is just one example and may be said to be able to manufacture the enhancer 321 in the form of a film through all methods that are currently developed and commercialized or can be implemented according to future technological developments. In addition, the material of the film is just one example, it will be said that the film of the present invention can be formed of a material capable of depositing all magnetic materials that are currently developed and commercialized or can be implemented according to future technology development.

In this embodiment, the enhancer 321 is preferably attached to the upper surface of the holder 310. More specifically, the enhancer 321 is attached to the upper surface of the holder 310. When the sample accommodating part 430 is fixed to the holder 310 in such a manner that the sample accommodating part 430 is inserted into the holder 310, the magnet accommodating part is detected in the upper surface specific area A of the holder 310 corresponding to the detection area 431 of the sample accommodating part 430. It is preferable to attach the enhancer 321 so that the signal amplification line 323 is located. This is to amplify only the magnetic signal of the sample contained in the detection area 431 to perform accurate measurement.

Accordingly, even when the sample is a low concentration sample and the amount of magnetic particles is distributed in the detection region, quantitative and accurate measurement is possible. In addition, since the enhancer 321 is coupled to the holder 310 and fixed at a specific position, even when the sample accommodating part 430 is changed, the enhancer 321 is disposed at the correct position and there is no change in the position of the magnetic material included in the enhancer. Possible errors can be reduced.

8 shows a holder according to a second embodiment of the present invention.

7 and 8, in this embodiment, the enhancer 325 includes a magnetic signal amplification line formed through etching after depositing a magnetic material directly on the upper surface of the holder 310. That is, instead of depositing a magnetic material on the film and attaching the magnetic material to the holder 310 as in the first embodiment of FIG. 7, the magnetic material is directly deposited on the upper surface of the holder 310 to generate an enhancer 325 including a magnetic signal amplification line. That's the way it is. When the enhancer 325 is fixed to the holder 310 in such a manner that the sample receiving part 430 is inserted, the enhancer 325 corresponds to the detection area 431 of the sample receiving part 430 similarly to the first embodiment of FIG. 7. It is preferable to be generated in the upper surface specific region A of the holder 310, and the description thereof is the same as described above in FIG. Accordingly, even if the sample is a low concentration sample and the amount of magnetic particles is distributed in the detection area, it is possible to measure quantitatively and accurately, and there is no change in the position of the magnetic material included in the enhancer during the measurement process, thereby reducing the error that may occur during measurement. Can be.

9 shows a holder according to a third embodiment of the present invention.

7 to 9, in this embodiment, the enhancer 327 is configured in the form of a capillary tube. More specifically, the capillary tube formed with a tubular passage is configured to be filled with a magnetic hardening filler containing magnetic material. At this time, the self-curing filler is composed of a UV-curing filler with a magnetic substance, and comprises Poly (Ethylene Glycol) -Diacrylate (PEG-DA) and 2-Hydroxy-2-methyl-1-phenyl-propan-1-one Can be. Magnetic material according to the present embodiment is preferably used the same as the magnetic particles to be used in the actual sample accommodating portion 430. That is, when the magnetic material of the same component as the magnetic particles of the sample accommodating part 430 to be actually used for the measurement is made of a capillary tube, the magnetic property can be accurately simulated. A more detailed response to the enhancer 327 in the form of a capillary tube is described in the description of FIG. 10.

In this embodiment, when the enhancer 327 is fixed in a manner such that the sample receiving portion 430 is inserted into the holder 310 similarly to the second embodiment described with reference to FIG. 8, the sample receiving portion 430 of FIG. 7 is fixed. It may be attached to the upper surface specific region (A of FIG. 8) of the holder 310 corresponding to the detection region 431 of FIG.

In addition, the holder 310 may further include an enhancer inserting portion 313 having a space for inserting an enhancer on the upper surface thereof. In this case, the enhancer 327 in the form of a capillary tube is inserted into the enhancer inserting portion 313. And may be coupled to the holder 310. At this time, it is preferable that the enhancer inserting portion 313 is formed in the above-mentioned A region. This is to amplify only the magnetic signal of the sample accommodated in the detection area 431 of the sample accommodating part 430 to perform accurate measurement.

Accordingly, even when the sample is a low concentration sample, it is possible to measure quantitatively and accurately. Even if the sample accommodating unit 430 is changed, the enhancer 327 is disposed in the correct position to reduce the error that may occur when measuring the signal.

FIG. 10 shows a method of manufacturing an enhancer in the form of a capillary tube of FIG. 9. 9 and 10, an enhancer 327 in the form of a capillary tube is manufactured as follows. First, as shown in (a) of FIG. 10, a capillary tube 610 having a constant cross section and having a hollow structure is formed with a tubular passage. The capillary tube may be made of various materials such as synthetic resin, glass, and the like, but here, the glass capillary is described as an example, but is not limited thereto. In this case, the cross section of the capillary tube may be any one of a circle, an oval, and a rectangle. It may have a shape of, in the present embodiment uses a tube having a rectangular cross section, but is not limited thereto.

Thereafter, the magnetic solution 620 is injected into the capillary tube 610 as shown in FIG. 10 (b). The magnetic solution 620 is generally a solution component including a magnetic material in the form of magnetic nanoparticles, and more preferably, a curing filler which may be cured by heat, light, ultraviolet rays, etc. after being injected into the capillary tube 610. May be included. In this embodiment, it is possible to solidify the magnetic material contained therein by using an ultraviolet curing filler. For example, the magnetic solution 620 may include Poly (Ethylene Glycol) -Diacrylate (PEG-DA) and 2-Hydroxy-2-methyl-1-phenyl-propan-1-one, and the magnetic nanoparticles May be further included. In particular, the solution containing Poly (Ethylene Glycol) -Diacrylate (PEG-DA) and 2-Hydroxy-2-methyl-1-phenyl-propan-1-one in the total magnetic solution is 60 to 70% by weight, magnetic nanoparticles The solution containing may be formed in a proportion of 30 to 40% by weight.

Thereafter, as shown in FIG. 10 (c), the capillary tube 610 filled with the magnetic solution 620 is cured to form a magnetic hardening filler having the magnetic solution 620 cured inside the capillary tube 610. (Hereinafter, the substance in which the magnetic solution is solidified is defined as 'self-curing filler'). In particular, the curing method in Figure 10 (c) may be applied to the thermosetting, photo-curing, etc., in this embodiment, but using an ultraviolet curing method, but is not limited thereto.

Thereafter, as shown in (d) of FIG. 10, the end of the capillary tube 610 may be sealed with a sealing material 630 such as epoxy to complete the capillary-type enhancer 327 according to the present embodiment.

Magnetic material according to the present embodiment is preferably used the same as the magnetic particles to be used in the actual sample accommodating portion 430. That is, when the magnetic material of the same component as the magnetic particles of the sample accommodating part 430 to be actually used for the measurement is made of a capillary tube, the magnetic property can be accurately simulated.

11 shows an example of the operation of measuring the output signal of the sample with the diagnostic device according to the present invention. In FIG. 11, an operation example according to the first embodiment of FIG. 7 is illustrated and described. However, this is only one example, and the overall operation example may be equally applicable to other embodiments.

7 and 11, the upper surface of the holder 310 is attached to the enhancer 321 in the form of a film, the enhancer 321 is a magnetic signal amplification line 323 formed through the deposition and etching of the magnetic material is It is provided.

The sample accommodating part 430 is provided with a detection area 431, and the detection area 431 accommodates a sample combined with magnetic particles.

When the sample accommodating part 430 is coupled to the holder 310, the magnetic signal amplification line 323 may be positioned at a position corresponding to the detection area 431. That is, the enhancer 321 is preferably disposed such that the magnetic signal amplification line 323 is positioned on the vertical lower surface of the detection area 431 when the holder 310 and the sample accommodating part 430 are coupled to each other.

When the sample accommodating part 430 is fixed to the holder 310 for diagnosis, the magnetoresistive sensor 200 moves on the sample accommodating part 430 through the driving module or the holder fixing the sample accommodating part 430 ( 310 moves below the magnetoresistive sensor 200. The magnetoresistive sensor 200 measures the output signal of the magnetic particles accumulated in the detection area 431 of the sample accommodating part 430. If the output signal measurement value of the existing detection area 431 is the voltage Vt, and the output signal measurement value of the enhancer 320 only is the voltage Ve, the enhancer 320 attached to the holder 310 in the case of the diagnostic kit according to the present invention. The output signal measured value is amplified to Vt + Ve and measured.

The amplification by the enhancer 320 amplifies only the output signal from the detection area 431 without changing the magnitude of the ambient noise, so that the signal can be distinguished even when the sample is a low concentration sample, and finally the signal-to-noise ratio of the diagnostic device. Can be increased, which enables more reliable measurement.

12 and 13 are graphs showing the output signal measurement value of the specimen with or without the enhancer of the present invention. More specifically, FIG. 12 is a graph showing output signal measurement values when no enhancer is provided, and FIG. 13 is a graph showing output signal measurements when an enhancer is provided. The X-axis shows the position of the sensor, the Y-axis shows the sensor output, and each response curve shows the sensor output for each concentration of the sample.

Referring to FIG. 12, when the concentration of the deployed sample is less than 0.02%, it is impossible to distinguish the maximum value of the graph for each position during the sensor scan, and the output signal measurement value is only an output level of the noise signal.

 On the other hand, referring to the case of FIG. 13 having an enhancer, it may be possible to distinguish the signal using the height of the signal for each position during each sensor scan until the developed sample concentration is 0.005%.

In other words, the diagnostic device of the present invention includes an enhancer for amplifying a magnetic signal in a holder of the diagnostic device, thereby improving performance, so that the signal can be distinguished even when the sample concentration is low, and even if the distance between the sensor and the developed sample becomes far. Signal classification is possible, and finally, the signal-to-noise ratio of the diagnostic device can be increased, so that more reliable measurement is possible.

As described above and described with reference to a preferred embodiment for illustrating the technical idea of the present invention, the present invention is not limited to the configuration and operation as shown and described as such, without departing from the scope of the technical idea It will be understood by those skilled in the art that many variations and modifications to the present invention are possible. Accordingly, all such suitable modifications and variations and equivalents should be considered to be within the scope of the present invention.

10: Diagnostic device
100: external magnetic field applying device 200: magnetoresistance sensor
300: drive module 310: holder
313: enhancer insertion unit 320: enhancer
321: film type enhancer 323: magnetic signal amplification line
325: magnetic signal amplification line type enhancer
327: capillary tube type enhancer
330: transfer unit 340: X axis drive motor
350: guide rail 360: support unit
370: Z axis drive motor 410: Sample
430: sample receiving unit 431: detection area
500:
610: capillary tube 620: magnetic solution
630: tube sealant

Claims (18)

A holder for fixing the sample receiving part;
An enhancer having a magnetic material for amplifying a magnetic signal and coupled to the holder;
Including,
The sample accommodating part is provided with a detection region, the diagnostic device using a magnetoresistive sensor for receiving a sample coupled to the magnetic particles.
The method according to claim 1, wherein the enhancer,
A magnetic signal amplification line formed by etching the magnetic material on a film to a predetermined thickness;
Diagnostic device using a magnetoresistive sensor comprising a.
The method according to claim 2,
The magnetic material is a diagnostic device using a magnetoresistive sensor containing at least one of Fe, Mn, Ni, Co.
The method according to claim 2, wherein the enhancer,
Diagnostic device using a magnetoresistive sensor is attached to the upper surface of the holder.
The method of claim 4,
The enhancer is attached to the upper surface of the holder,
When the specimen receiving portion is fixed to the holder, the magnetic signal amplification line is attached to an area corresponding to the detection area so as to be positioned. Diagnostic device using magnetoresistive sensor.
The method according to claim 1, wherein the enhancer,
Diagnosis device using a magnetoresistive sensor consisting of a magnetic signal amplification line formed by etching after depositing the magnetic material to a predetermined thickness on the upper surface of the holder.
The method of claim 6, wherein the magnetic signal amplification line,
And a magnetoresistive sensor formed on an upper surface of the holder corresponding to the detection area when the specimen receiving part is fixed to the holder.
The method according to claim 1,
The enhancer,
Capillary tubes formed with tubular passages;
Self-curing filler is filled in the capillary tube and the magnetic material is added;
Diagnostic device using a magnetoresistive sensor comprising a.
The method according to claim 8,
The magnetic hardening filler is a diagnostic device using a magnetoresistive sensor comprising a ultraviolet curing filler to which the magnetic material is added.
The method according to claim 9,
The magnetic hardening filler is a diagnostic device using a magnetoresistive sensor comprising Poly (Ethylene Glycol) -Diacrylate (PEG-DA) and 2-Hydroxy-2-methyl-1-phenyl-propan-1-one.
The method of claim 10,
The capillary tube is a diagnostic device using a magnetoresistive sensor formed in a structure in which both ends are sealed with a sealing material.
The method according to claim 8,
The magnetic body is a diagnostic device using a magnetoresistive sensor formed of the same material as the magnetic particles.
The method according to claim 8,
And the enhancer is attached to an upper surface of the holder corresponding to the detection area when the specimen receiving part is fixed to the holder.
The method according to claim 8,
The holder may include an enhancer insert formed on an upper surface of the holder and provided with a space for inserting the enhancer; Including,
The enhancer is a diagnostic device using a magnetoresistive sensor is inserted into the enhancer insertion portion and coupled to the holder.
The method according to claim 14,
And the enhancer inserting portion is formed on an upper surface of the holder corresponding to the detection area when the specimen receiving portion is fixed to the holder.
The method according to any one of claims 1 to 15,
An external magnetic field applying device for applying an external magnetic field to the specimen;
A magnetoresistive sensor for detecting a magnetic component of the sample according to the external magnetic field;
Diagnostic device using a magnetoresistive sensor further comprising.
18. The method of claim 16,
The external magnetic field applying device is a diagnostic device using a magnetoresistive sensor including any one or more of a solenoid coil, a Helmholtz coil, an electromagnet yoke.
18. The method of claim 16,
The magnetoresistive sensor is a diagnostic device using a magnetoresistive sensor, characterized in that composed of at least one giant magnetoresistive (GMR) device.

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