KR101390347B1 - Detergent composition comprising Sicyos angulatus extract as effective component - Google Patents

Abstract

The present invention relates to a detergent composition having an antimicrobial effect, which contains an extract as a active ingredient. More particularly, the present invention relates to a detergent composition having an antibacterial effect, To an excellent detergent composition.

Description

[0001] The present invention relates to a method for preparing a hand cleansing agent using a composition containing an extract of Aspergillus as an active ingredient,

More particularly, the present invention relates to a detergent composition comprising an extract of Rhodochrosophila melanogaster as an active ingredient, and more particularly, to a detergent composition containing an extract of Rhodochrosophila melanogaster extract as an active ingredient without toxicity or side effects, The present invention relates to a method for preparing a hand detergent using a composition having excellent antibacterial effect against Staphylococcus aureus.

Sicyos angulatus is a one or two year old vine plant belonging to the genus Bacillus. It grows in the beaches and foothills of the central and southern parts of Korea, or in the land with water or moisture. Origin is North America and is a naturalized plant. The stem is 4 ~ 8m long and clings to other objects with a tendril. The palm-shaped leaves with 5 to 7 edges split off the stem. The female flower blooms in June to September as one male and female flowers. The female flower is gathered in a morphiform inflorescence which is a round head bloom, and the male flower blooms in a green color on the flower-like inflorescence. There are many hairs on the stem of the flower, and several fruits are gathered together and covered with thin thorn like fur, and the flower is pale green.

In general, the skin of a person is decomposed to the skin surface through sebaceous pores in the sebaceous gland, thereby suppressing the water evaporation of the skin and maintaining the elasticity. However, due to abnormal keratinization of the skin epithelium, the pores become clogged, and if sebum does not escape to the skin surface, the sebaceous glands become clogged in the pores. When anaerobic bacteria penetrate into the pores, inflammation occurs and the skin becomes reddened.

In general, the infection path of bacteria is considered as a hand. We often wash our hands to kill these germs. Bacteria in the hands are rubbed by hand only without the detergent, which means that even if only the physical force is applied, the bacterium can be removed to some extent, but we usually wash the hands with the soap bar. The sterilization process of the soap takes advantage of the surface activity of the soap, and it is effective to wash it for more than one minute. However, the average hand washing time is about 10 seconds. And after about an hour, the germs between the skin pores again move out of the skin, causing many bacteria to reside in the skin.

In particular, various methods have been taken in the past to clean and sterilize the hands of workers in the food-related industry, such as food processing, processing seafood, or cooking in restaurants, physicians, nurses, caregivers, and patients such as hospitals or public health centers. The most common method is to wash the hands thoroughly with soap or the like, and then subject to a sterilization treatment. Examples of the sterilization method include a method of immersing a hand in a disinfectant or spraying a disinfectant. However, since the treatment sequence is divided into two steps of washing and sterilizing the hands, it takes too much time, the two-step operation tends to become uncertain, and furthermore, when either washing or sterilization is incompletely performed, And the like. Even if the sebum on the surface of the skin is well cleaned, the microorganisms in the air will grow on the surface of the skin after the cleaning, and the skin will become sickened by the proliferation of the skin surface, and the smell of the skin will be deteriorated. Normally, But it is not perfect.

Korean Patent Publication No. 2012-0063036 discloses a herbal hand cleanser composition comprising a herbal extract, anhydrous ethanol, aloe vera gel, hyaluronic acid, purified water, grapefruit seed extract, and essential oil. In Korean Patent No. 1136184, Discloses an antimicrobial detergent composition comprising a component selected from the group consisting of an octagonal fennel, tungsten oxide, copper chloride, platinum chloride, lithium silicate, molybdenum oxide or rhodium and distilled water. However, in the prior art, there is no description about a detergent composition having an excellent antimicrobial effect by using the extract of Thornberry as in the present invention.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs. Accordingly, it is difficult to remove the visible mark disturbing the ecosystem of the present country. The inventor of the present invention has found that, As a result of studying through trial and error for a long period of time, it has been found out that a new antibacterial effect of spiny roots is not only revealed, but also a method of maximizing the antimicrobial effect of the spike roots is pre- Which is free of irritation and toxicity to the skin and has a high antibacterial activity.

In order to solve the above problems, the present invention is in Vibrio containing visible foil extract as an active ingredient (Vibrio spp.), Pseudomonas sp. spp.), genus Shigella (Shigella spp.), Staphylococcus genus (Staphylococcus spp.) or Escherichia The present invention provides a detergent composition excellent in antibacterial effect against E. coli .

The detergent composition containing the extract of the present invention is excellent in the degree and amount of bubbles during use, has a smooth surface, has excellent skin absorption, has no skin irritation before and after use, has excellent moisture retention , And the stability of physical properties is excellent. In addition to the cleaning effect, Vibrio spp.), Pseudomonas sp. spp.), genus Shigella (Shigella spp.), Staphylococcus genus (Staphylococcus spp.) and Escherichia coli and the like can be helpful in alleviating or improving skin diseases such as various eczema which are infected by bacteria and fungi.

FIG. 1 is a photograph showing a manufacturing process of a visible thin sample.
(a) Spinach juice, (b) Visible powder, (c) Cooked boiled water, (d) Spinach ethanol extract
Fig. 2 shows a Petri dish cultured for 24 hours after inoculating the culture medium with the cultured bacteria (colonies) without treating the sample with visible samples.
Fig. 3 shows a Petri dish inoculated with the cultured bacteria (colonies) and cultured for 24 hours after being treated with ethanol extract of the viscous pet.
Fig. 4 shows a petri dish inoculated with the cultured fungus (colonies) and cultured for 24 hours after treating the pak chrysanthemum.
Fig. 5 shows a petri dish inoculated with cultured fungi (colonies) on a medium and cultured for 24 hours after boiled water was treated.
Fig. 6 shows a Petri dish inoculated with the cultured bacteria (colonies) and cultured for 24 hours after treatment with the thin powder.
In the photographs of FIGS. 2 to 6, the upper petri dish uses TCBS medium, and the lower petri dish uses TSA medium. In addition, the first petri dish bacteria from the leftmost petridish were Vibrio fluvialis , the second petri dish bacteria are Pseudomonas oryzihabitans , the third petri dish bacteria are Shigella spp., and the fourth Petri dish shows that the bacteria were not grown.
FIG. 7 is a photograph of Staphylococcus aureus (Staphylococcus aureus) and Escherichia It is a photograph comparing the size of antibiotics treated with antibiotics to the culture medium of E. coli .
Figure 8 shows that Staphylococcus aureus (Staphylococcus aureus) and Escherichia This is a photograph of the antimicrobial size of the coli (Escherichia coli) treated with the ethanol extract of the barnyard grass.

In order to achieve the object of the present invention, the present invention provides a detergent composition having an antimicrobial effect containing an extract of Spinach as an active ingredient.

In the detergent composition of the present invention, the visible leaf may be a visible leaf, and the visible leaf has an excellent antimicrobial effect compared to other areas (such as stems) of the visible leaf so that bacteria and fungi can be effectively controlled There is an advantage to be able to.

In addition, in the detergent composition of the present invention, the visa free extract may be a visible ethanol extract. More specifically, 800 to 1200 ml of ethanol is added to 100 to 200 g of dried persimmon, and the mixture is extracted for 1 to 3 hours. And then dried at 34 to 40 ° C. More specifically, 1000 g of ethanol is added to 150 g of visible powder, which is then extracted for 2 hours, filtered, evaporated to remove ethanol, and dried at 37 ° C. But are not limited thereto.

In addition, the extract of the present invention can be prepared by concentrating the ethanol extract obtained by dissolving the leaves of prickly leaves in ethanol at 40 to 50 ° C for 1 to 3 hours, more preferably by dissolving ethanol in ethanol The extract may be prepared by concentrating at 45 [deg.] C for 2 hours, but is not limited thereto. It was possible to prepare an extract of Prunus persica, which is superior in antimicrobial effect to other pretreatment methods, by preparing the extract of Prunus persica.

In addition, in the detergent composition of the present invention, the antimicrobial effect may be inhibited by Vibrio spp.), Pseudomonas sp. spp.), genus Shigella (Shigella spp.), Staphylococcus genus (Staphylococcus spp.) or Escherichia can be a anti-bacterial effect on coli), the genus Vibrio (Vibrio spp.) is preferably selected from the group consisting of Vibrio fluvialis ), the Pseudomonas sp. spp.) is preferably a Pseudomonas sp. oryzihabitans ), Staphylococcus sp. spp.) is preferably, but not limited to, Staphylococcus aureus .

In the detergent composition of the present invention, the visbreaker extract may be contained in the detergent composition in an amount of 0.01 to 50% by weight, preferably 3 to 20% by weight. When the amount is less than the above range, And if it exceeds the above range, it is difficult to form a cleaning agent formulation.

In addition, in the detergent composition of the present invention, a stabilizer may be used for stabilizing the ingredients of the viscose oil extract as an effective ingredient of the detergent composition. The stabilizer may be selected from the group consisting of vitamin C, citric acid, sodium citrate, malic acid, sodium carbonate, sodium bicarbonate, sodium hypophosphite, sodium bisulfite, tocopherol acetate, tocopherol acetate, sodium ascorbate, sodium bisulfite, anhydrous sodium sulfite, , Glutathione, polyphenol, butylated hydroxyanisole, butylated hydroxytoluene, ascorbyl palmitate, ascorbyl oleate, polysorbate 80, onlain acid, DBA (alkylbenzenesulfonic acid ), But is not limited thereto. It is preferable that the stabilizer is contained in the composition in an amount of 0.1 to 5% by weight, preferably 0.1 to 2% by weight. If it is used in an amount less than the above range, a sufficient stabilizing effect can not be expected. If it is used in excess of the above range, side effects such as skin irritation may occur.

Further, in the detergent composition of the present invention, a carrier component may further be contained in the detergent composition. For example, the metal ion sequestering agent may be added to the composition in an amount of not more than about 0.2% by weight, and flavoring agents, dyes, pigments and the like may be added at a level of about 6.0% by weight or less.

Further, in the detergent composition of the present invention, the detergent composition may be, but not limited to, a solid cosmetic soap, a hand cleaner, a liquid shampoo, a liquid soap, a liquid rinse, a body cleanser or a creamy soap.

When the solid cosmetic soap is prepared from the detergent composition, it can be produced according to a conventional conventional soap manufacturing method. For example, a cosmetic soap base containing water in an amount of 8 to 25 parts by weight in a certain mixer is put into a bowl, and an effective ingredient, an extract of Kashibak, is added to the mixture, followed by adding a flavor, dye and other additives. Or a mixture of two or more of them, followed by extrusion, molding and cutting.

The cosmetic soap base may be a sodium salt or a potassium salt of palm oil, which is a commonly used natural oil. Preferably, palm fatty acid sodium salt and potassium salt having 10 to 25 carbon atoms can be used.

Among the detergent compositions according to the present invention, about 50 to 98 parts by weight of a soap base containing 8 to 25 parts by weight of water can be used in the solid cosmetic soap. A moisturizing agent may further be added to the solid cosmetic soap. Examples of the moisturizing agent that can be used include jojoba oil, glycerin, polyethylene glycol (molecular weight 4000), and ethylene glycol in an amount of 0.1 to 5% by weight in the soap.

The detergent composition of the present invention is an environmentally friendly composition which is free from toxicity and side effects even when used everyday. In particular, it has an excellent antibacterial effect in addition to a cleaning effect and can exhibit excellent therapeutic effect and hygienic feeling for most skin diseases caused by fungi have.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Experimental Example  One: Thorny night  Bacterial inhibition of processed samples

1. For antimicrobial effect measurement Thorny night  Preparation of sample

(1) 150 g × 4 set of visible pellets, 500 ml of distilled water, 150 ml of ethanol, 150 ml of water, 2 500 ml of beaker, alcohol lamp, trivet, mulberry bowl, Mullet, green juice, 20 Erlenmeyer flasks (100 ml) and scales were used.

2) Making samples using visible mark

The samples using spiny foil were prepared as juice form, powder, boiled water and ethanol extract of spiny rice according to the processing method.

(A) 150 g of prickly pear is cleanly washed with 500 ml of distilled water and made into juice by using a green juice. Then, 50 ml of prickly pear juice (150 g of prickly pear → 100 ml of prickly pear) is added to the sterilized bottle (Fig. 1 (a)).

B.) Preparation of baking powder: 150 g of prickly pear is washed with clean water, dried in a cool place for about 20 days, and then pulverized into powder using a mortar bowl (50 g of baking powder → 50 g of baking powder) (Fig. 1 (b)).

C) Preparation of boiled rice boiled water: 150 g of cooked rice was put into a beaker with the same amount of water and boiled for 2 hours to prepare a sample (Fig. 1 (c)).

D) Preparation of ethanol extract of spiny foil: 150 g of spiny foil was placed in a beaker with 1000 ml of ethanol, heated with a hot water bath in an alcohol lamp for 2 hours, and then the residue was removed. The ethanol extract from which the residue was removed was filtered through a filter paper, and the ethanol was gradually evaporated by a vacuum pump. The concentrate was dried at 37 ° C and the remaining powder was dissolved in 0.85% physiological saline to prepare an extract (see d)).

The four visible samples prepared as described above were stored in a -20 ° C freezer until the experiment of bacterial effect.

2. Thorny night  Examination of bacterial inhibition effect of samples

1) Make a badge for bacterial experiment

Add 40 g of reagent (BD Difco ^™ Tryptic Soy Agar) per 1 L of distilled water (DW) to the Erlenmeyer flask, and add TSA medium by adding the steal bar. (When the bacteria isolated from seawater is cultured, 1.5% sodium chloride Was added. In addition, 89 g of reagent (BD Difco ^™ TCBS Agar) per 1 L of distilled water (DW) was added to the Erlenmeyer flask, and then the TCBS medium was prepared by adding the steal bar and stirring.

The upper part of the Erlenmeyer flask containing the TSA medium and TCBS medium prepared above was covered with a foil and then labeled. Then, the reagent was sufficiently dissolved at about 50 ° C or higher and about 400 rpm, and sterilized by putting it in a sterilizer. After sterilization, the medium was slightly cooled and poured about 20 to 25 ml into a Petri dish. At this time, gloves were used to hold the Erlenmeyer flask, and sterilized when Kimtall was used. The Petri dish lid was then opened and completely dried, and the Petri dish was wrapped in plastic wrap and placed in a zipper pack and refrigerated.

2) Isolation and Culture of Bacteria

A) Inoculation of sea water: After transferring the seawater collected at the beach to the laboratory, it was firstly inoculated into the prepared medium and cultured in a 25 ° C incubator for a day. After 24 hours, the cultured bacteria (colonies) were detached using disposable platinum beads, and then re-inoculated into new medium (TCBS, TSA) and cultured for 24 hours.

(B) Preparation of Fungus: Using a disposable platinum beet, one of the bacteria (colonies) grown in the medium was placed in a 50 ml plastic test tube together with 0.85% physiological saline, and the mixture was thoroughly mixed and prepared with a stirrer. In this way, four kinds of bacterial solutions were prepared.

C) Preparation of Bacterium Medium: 200 μl of each prepared bacterium was dropped on a medium (TCBS, TSA) using a micropipette, and then spread with a spreader to completely absorb it.

D) Treatment of astringent samples: 25 μl or 25 mg of four kinds of visible samples (ethanol extract, juice, boiled water, powder) were dropped on the bacterial medium in the center of the culture medium, and incubated in an incubator for 24 hours at 25 ° C Lt; / RTI > The control group used bacillus thuringiensis without treatment of thymus.

3) Confirmation of bacterial inhibition effect

After 24 hours, the control group and the control group were taken out of the incubator, and the portions where bacteria did not grow in each medium were identified.

4) Identification of bacteria

An API-20 kit was used for bacterial identification.

A) Bacteria No. 1: Vibrio It is widely distributed in coastal waters, and it causes food poisoning mainly through fish and shellfish. In food poisoning caused by this bacterium, infection occurs by mixing with Vibrio parahaemolyticus. - Symptoms: Vomiting, Abdominal pain, diarrhea)

B) Bacteria 2, 4: Pseudomonas oryzihabitans the bacteria belonging to the (four genera and hundreds of large and diverse syudomonaseugwa (Pseudomonadaceae made of species), causing antibiotic-resistant infection to mild resistance People surgical wounds or serious burns tissue. Hospitals are known to cause acquired infections and cause fatal infections in cancer patients treated with immunosuppressive drugs.)

C) Bacteria No. 3: Shigella It is a genus of rod-shaped bacteria belonging to Enterobacteriaceae (Enterobacteriaceae). It lives in the intestine of a normal person and causes dysentery or shigellosis. It is a common infection in a poorly developed country, caused by a bacteria called shigella , which is usually infected by contact with bacteria in the hands of patients or carriers.

Experimental Example  2: Thorny night  Bacterial Inhibition of Ethanol Extract Staphylococcus aureus , Escherichia coli ) Review the effect

Ethanol extracts of spiny leaves have been shown to be effective against bacterial inhibition, and thus have been shown to have some effect on the staphylococcus aureus and E. coli, which are likely to occur in our daily life. Experiments were performed to compare the bacterial inhibitory effect of ethanol extracts on the stalk (stem, leaf, stalk + leaf).

1) Experimental process

A) Preparation and concentration of ethanol extract of Spiracetum: Each of the samples (leaves, stems, leaves, stems) of the spirits was dissolved in ethanol to prepare an ethanol extract. The ethanol extract (70 ml) was added to the concentrate (1 liter) and heated to 45 ° C in a constant temperature water bath to adjust the pressure without boiling. The concentrate was concentrated to take a thick solution (about 2 hours). Approximately 20 ml of ethanol was added using a syringe and the dried material was dissolved. A small concentrator is used because it is difficult to recover the final product when it is dried from a large concentration column to the end. Approximately 5 ml of the solution dissolved in a small concentrated sample bottle was added and the mixture was concentrated under reduced pressure. When concentrating under reduced pressure, add about 5 ml to prevent boiling due to decompression, and finally dry.

2) Investigation of the antibacterial activity (Paper disc experiment)

A) Culture of experimental bacteria: The target microorganism was Staphylococcus aureus (Staphylococcus aureus), Escherichia coli (E. coli) was used. A single colony was isolated from the medium of each microorganism (bacterium), inoculated into 5 ml of liquid medium (LB), and cultured at 37 占 폚 for 12 hours to prepare bacteria.

B. Preparation of Bacterium Medium: LB medium was prepared by pouring LB medium into a Petri dish. Each of the cultured bacterial liquids was uniformly plated on a surface of a bacterial medium (LB medium) with a cotton swab by a micropipette at a rate of 100 μl, followed by drying for 30 minutes so that the liquid was sufficiently permeated.

C) Preparation of antimicrobial disc: 0.02 g of the extract powder (3 kinds of leaves, stems, leaves and stems) was added to 1.5 ml microtube and 200 μl of DMSO (dimethy sulfoxide) was mixed and dissolved sufficiently All to match 100 mg / ml). Three kinds of the soluble extracts were absorbed by 10 μl, 30 μl, and 50 μl each on a paper disk (diameter 6 mm) to prepare final concentrations of 1, 3 and 5 mg per disc. The fabricated discs were dried on a clean bench for 30 minutes.

D) Antimicrobial activity test: Thick discs prepared for each concentration were placed on a bacterial culture medium and cultured at 37 ° C for 10 hours. The diameters of the zona pellucida (antimicrobial or inhibitory rings) formed around the disc were measured using a ruler.

Streptomycin was used as an antibiotic, and E. coli and S. aureus were used as bacterial strains. Staphylococcus aureus (Staphylococcus aureus ) The antimicrobial activity (antimicrobial activity) was measured in the same manner as in the above experiment.

Example  One: Thorny night  Bacteria inhibitory effect of sample

Bacterial inhibitory effects of the four kinds of silkworm samples prepared by the method of Experimental Example 1 are shown in Table 1 and Figs. 2 to 6 by bacteria and medium. As a result, the ethanol extract of barnyard bark effectively inhibited the growth of bacterium Vibrio, Pseudomonas and Shigella compared to the control (Fig. 2), and the ethanol extract was effective on both TCBS and TSA medium of each bacterium (Fig. 3).

The spiny berry juice showed no effect in TCBS-grown bacterium Vibrio, Pseudomonas (Fig. 4). Water and powder boiled in water showed no effect on bacterial inhibition (Figs. 5 and 6), and viscous powder was found to be infected during storage (Fig. 6).

Experimental results showed that the viscose was effective in inhibiting bacterial growth when made from ethanol extract or juice, and was not effective in bacterial inhibition when prepared with boiled water or powder. Therefore, ethanol extracts should be more effective than ethanol extracts for the purpose of sterilization or bacterial inhibition.

Bacterial inhibition effect of visible bacteria on four kinds of bacteria A visible sample Bacteria-1 Bacteria-2 Bacteria-3 Bacteria-4 TCBS TSA TCBS TSA TCBS TSA TCBS TSA Ethanol extract ○ ○ ○ ○ ○ ○ - - Juice × ○ × ○ ○ ○ - - Boiled water × × × × × × - - powder × × × × × × - - ※ Bacteria-4 did not grow bacteria.
※ ○, with bactericidal effect; ×, no bactericidal effect; - Germs did not grow

Bacteria-1: Vibrio fluvialis

Bacteria-2: Pseudomonas oryzihabitans

Bacteria-3: Shigella spp .

Example  2: Thorny night  Antibacterial effect of ethanol extract on bacteria (Escherichia coli, Staphylococcus aureus)

The antimicrobial activities of E. coli and Staphylococcus aureus were compared in Table 3 and compared with the antimicrobial effect of streptomycin as a control (Table 2) .

As a result, it was found that antimicrobial component inhibiting bacteria was contained in the leaves of Rhizopus spp., And antimicrobial component was not contained in the stem of Rhizopus spp. The antimicrobial activity was also increased with increasing concentrations of 3 mg / ml and 5 mg / ml.

The antimicrobial activity of Spinach was 2 ~ 3 times higher than that of Staphylococcus aureus. For comparison with astringent leaves, 1 ~ 10 ㎍ of antibiotic (streptomycin) was treated with Escherichia coli and Staphylococcus aureus and showed similar antibacterial activity to 1 ~ 5 mg / ml of astringent leaf extract (Figs. 7 and 8) .

Comparison of antibacterial effect of antibiotic (streptomycin) (unit of antibacterial size in mm) Antibiotic concentration E. coli Staphylococcus aureus ( S. aureus ) 1 ug / disc 10 9 2 ug / disc 14 13 10 ug / disc 20 18

Antibacterial Effect of Ethanol Extract of Spiny Rice against Bacteria (Unit of Antimicrobial Size: mm) Thorny night
density E. coli Staphylococcus aureus ( S. aureus ) leaf stem Leaf + stem leaf stem Leaf + stem 1 mg / ml 4 - 3 12 - 6 3 mg / ml 8 - 7.5 21 - 17 5 mg / ml 10 - 9 24 - 23

3. Thorny night  Preparation of detergent using ethanol extract

The antimicrobial effect of the ethanol extract of Spirax Saraf was confirmed against the bacterium.

Formulation Example  1: solid cosmetic soap

3 to 5 parts by weight of ethanol extract of barnyard grass of Experimental Example 1 or 3 to 5 parts by weight of ethanol extract of barnyard leaf of Experimental Example 2, 0.2 parts by weight of glycerin, 0.8 parts by weight of polyethylene glycol 4000, 95.25 to 93.25 parts by weight of soap base, 0.3 part by weight of tocopherol acetate, 0.3 parts by weight of jojoba oil and 0.15 parts by weight of cetanol were mixed well in a mixer, extruded in a soap making machine, cut into a certain size and extruded to prepare a solid cosmetic soap composition.

Formulation Example  2: hand cleaner composition

5 to 15 parts by weight of ethanol extract of barnyard grass of Experimental Example 1 or 5 to 15 parts by weight of ethanol extract of barnyard leaf of Experimental Example 2, 0.3 parts by weight of tocopherol acetate, 0.2 parts by weight of glycerin, 0.4 parts by weight of alkylbenzenesulfonic acid, 10 parts by weight of soap base, , 0.1 part by weight of cetanol, 5 parts by weight of cetanol and 69 to 79 parts by weight of distilled water were mixed in a mixer to prepare a liquid form of a water soap composition.

Formulation Example  3: Shampoo composition

10 to 20 parts by weight of ethanol extract of barnyard grass of Experimental Example 1 or 10 to 20 parts by weight of ethanol extract of barnyard leaf of Experimental Example 2, 5 parts by weight of soap base, 0.3 part by weight of jojoba oil, 0.15 part by weight of cetanol, 1.0 part by weight of glutathione, 0.05 parts by weight of hydroxytoluene, 0.3 parts by weight of tocopherol acetate, 0.2 parts by weight of glycerin, 0.4 parts by weight of alkylbenzenesulfonic acid and 72.6 to 82.6 parts by weight of distilled water were mixed well in a mixer to prepare a liquid shampoo composition.

Formulation Example  4: Rinse composition

3 to 5 parts by weight of ethanol extract of barnyard grass of Experimental Example 1 or 3 to 5 parts by weight of ethanol extract of barnyard leaf of Experimental Example 2, 0.3 part by weight of jojoba oil, 0.15 part by weight of cetanol, 0.3 part by weight of tocopherol acetate, 0.2 part by weight of glycerin, 0.8 parts by weight of water and 93.25 to 95.25 parts by weight of distilled water were mixed in a mixer to prepare a rinse composition which is a liquid form.

Formulation Example  5: body  Detergent composition

10 to 20 parts by weight of the ethanol extract of Visible Pellets of Experimental Example 1 or the ethanol extract of Palladium Leaf of Experimental Example 2, 0.1 parts by weight of oleic acid, 0.15 parts by weight of cetanol, 1.0 part by weight of glutathione, 0.05 parts by weight of butylated hydroxytoluene, 0.3 parts by weight of tocopherol acetate, 0.2 parts by weight of glycerin and 78.2 to 88.2 parts by weight of distilled water were mixed in a mixer to prepare a liquid detergent composition.

The detergent composition containing the extract of Kashibak helps to reduce or improve skin diseases such as eczema which is infected by bacteria and fungi due to excellent antibacterial effect on Vibrio and E. coli as well as cleaning effect, , It has smooth surface texture, excellent skin absorption, no skin irritation before and after use, and can be used in a nature-friendly manner as a detergent using natural materials, and has industrial effects such as utilization of foreign plants.

Claims (6)

The extract is prepared by adding 800 to 1200 ml of ethanol to 100 to 200 g of prickly pear leaves, extracting it for 1 to 3 hours, filtering, removing the ethanol by evaporation and drying at 34 to 40 ° C., or preparing ethanol by dissolving 5 to 15 parts by weight of ethanol extract of prickly pear leaf extract prepared by concentrating the extract at 40 to 50 ° C for 1 to 3 hours, 0.3 part by weight of tocopherol acetate, 0.2 part by weight of glycerin, 0.4 part by weight of alkylbenzenesulfonic acid, parts of oleic acid, 0.1 part by weight of cetanol, 5 parts by weight, and in distilled water 69 to 79 parts by weight is made into a mixture by mixing, Vibrio (Vibrio spp.), Pseudomonas species (Pseudomonas spp.), Shigella genus (Shigella spp. ), Staphylococcus spp., Escherichia coli , and the like. delete delete delete delete delete

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