JPS61213771A - Liquid specimen dripping device - Google Patents

Abstract

PURPOSE:To obtain a liquid specimen dripping device capable of accurately performing chemical analysis without mixing other specimen, constituted so as to supply a definite amount of the liquid specimen received in a capsule by a pneumatic pressure in a liquid droplet form. CONSTITUTION:A capsule member 10 having a proper amount of a liquid specimen injected therein is inserted in a holder member 20 so as to protrude a dripping chip part 12 thereof from the opening 22 of said holder member 20. The capsule member 10 has a pair of large diameter parts 18 formed above and below the injection port 16 thereof to engage the outer wall of the capsule member 10 and the inner wall of the holder member 20 in an air-tight manner and the injection port 16 is communicated with an air supply port 24. When a proper amount of air is supplied from the air supply port 24, air enters the capsule member 10 from the injection port 16 to push down the liquid level of the liquid specimen and a liquid droplet having a proper size is formed to the lower end of the dripping part 12 and the supply of the liquid droplet in a liquid droplet form can be simply and rapidly performed.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention is a sample liquid spotter, more specifically, a sample liquid spotter for spotting and supplying a sample liquid for analysis to an analysis material for analyzing a specific chemical component. This relates to the equipment.

(Prior Art) Qualitatively or quantitatively analyzing a specific chemical component in a liquid sample is a commonly performed operation in various industrial fields. In particular, analyzing chemical components in biological body fluids such as blood and urine is extremely important in the biochemical and clinical fields.

In recent years, dry-type analytical materials have been developed that enable foot-to-mouth analysis of specific chemical components contained in a sample solution by simply applying small droplets of the sample solution (Japanese Patent Publication No. 53-2).
No. 1677, JP-A-55-164356, etc.)
It has been put into practical use. Using these analytical materials, sample liquids can be analyzed more easily and quickly than conventional wet analysis methods, so their use is particularly important in medical institutions, laboratories, etc. that need to analyze a large number of samples. This is preferable.

Also, JP-A-53-81292, JP-A-58-161
No. 867.

Japanese Unexamined Patent Publication No. 58-21566 and the like describe a chemical analysis apparatus for carrying out analysis using such analysis materials.

As mentioned above, in order to analyze specific components contained in a sample liquid using such analytical materials, it is necessary to apply an appropriate amount of the target sample liquid onto the analytical material. Preferably, a liquid spot delivery means is used.

At this time, especially when using a biological body fluid as a sample liquid, try to prevent the sample from coming into contact with the outside air as much as possible between the time of collection of the sample (or sample liquid) and the spotting of the sample due to evaporation, coagulation, contamination, etc. It is preferable for analysis to prevent changes, and it is desirable that the sample liquid spot supply means satisfy such conditions. In addition, in clinical tests, etc., it is common practice to analyze multiple chemical components by spot-feeding one specimen to multiple analysis materials for different chemical component analyses. It is desirable that the spot supply means be suitable for such operations. Furthermore, especially when a large number of specimens need to be analyzed quickly, it is desirable that the sample liquid spot supply means be such that one specimen will not be contaminated with another specimen.

However, at present, there is no known sample liquid spot supply means that satisfies the above-mentioned conditions, can be easily operated, and is easily manufactured.

(Objective of the Invention) In view of the above-mentioned current situation, the present invention provides a method that allows a sample solution to be spot-supplied onto any analysis material and that can be easily manufactured so that accurate chemical analysis can be performed simply and quickly. The purpose of this invention is to provide a sample liquid spotter that can perform the following functions.

(Structure of the Invention) The sample liquid spotter according to the present invention comprises a capsule member for holding a sample liquid and a holder member for removably housing the capsule member.

The capsule member is cylindrical with an open lower end forming a dripping tip and a closed upper end, with an inlet in the center thereof suitable for injecting sample liquid and air. Also, a pair of large diameter portions are formed above and below this injection port. When the capsule member is attached to the holder member, the dropping tip portion protrudes from the lower end of the holder member, and the outer wall of the large diameter portion and the inner wall of the holder member are airtightly fitted. Furthermore, a ventilation hole is provided in the center of the holder member, and this ventilation hole is located between the pair of large diameter parts when the capsule member is attached to the holder member, and sends air to the injection port. It is now possible to do so.

The "pair of large-diameter portions" provided in the capsule member each generally mean a ring-shaped portion having a larger diameter than other portions of the capsule member. It is also possible to configure each of the pair of large diameter portions with a plurality of ring-shaped portions, and in this case, the airtightness when the capsule member is attached to the holder member can be further improved.

It is also possible to improve the airtightness between the capsule member and the holder member by providing a small diameter part (protrusion) on the inner wall of the holder member that comes into close contact with the outer wall of the large diameter part of the capsule member. Depending on the shape, the large diameter portion of the capsule member may have approximately the same diameter as the other portions.

In the present invention, the "dropping tip section" is a small fIi (several μt
It means a tip portion having an opening through which a sample liquid of ~ several + μQ) can be dropped as a droplet.

Furthermore, in this specification, the term "sample liquid" refers to any liquid sample that can be used in any chemical analysis process, and includes not only a sample liquid containing chemical components of an unknown date to be analyzed. The so-called "reference solutions J and "
It also includes "standard solutions."

(Effects of the Invention) In the sample liquid dropper according to the present invention, the capsule member for holding the sample liquid is sealed except for the injection port and the opening at the tip of the dripping tip. If the sample liquid is injected directly into the container, the sample liquid can be reliably held inside the container without being affected by the outside air.

Furthermore, when the capsule member is attached to the holder member, the outer walls of the pair of large diameter parts of the capsule member and the inner wall of the holder member are airtightly fitted, and the injection port of the capsule member and the ventilation port of the holder member are communicated with each other. Therefore, by simply supplying an appropriate amount of air from the vent, it is possible to form droplets of sample liquid at the tip of the dropping tip of a size suitable for spot-feeding onto any analytical material. In addition, if a sufficient amount of sample liquid is injected into the capsule member in advance, the same sample liquid can be easily and quickly applied to multiple analysis materials by controlling intermittent air supply. It is possible to do so.

Furthermore, the capsule member is attached to the holder member I! Since it is designed to be removably housed, the sample liquid can be discarded or replaced together with the capsule member, and there is therefore no risk of different sample liquids mixing with each other.

As described above, the sample liquid spotter according to the present invention is capable of spotting sample liquid onto any analytical material so that accurate chemical analysis can be performed simply and quickly, and has a simple configuration. Therefore, it is easy to manufacture.

(Actual mode) Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1A and FIG. 1B are perspective views showing a capsule member and a holder member, respectively, which constitute a sample liquid spotter according to an embodiment of the present invention, and FIG. 2 is a perspective view showing the capsule member attached to the holder member. FIG. 3 is a side sectional view of the sample liquid spotter at the time of supplying the sample liquid.

The sample liquid spotter shown in these drawings consists of a capsule member 10 and a holder member 20. The capsule member 10 is a cylindrical member having an open lower end forming a dripping tip portion 12 and a closed upper end 14, and has an inlet 16 in the center thereof suitable for injecting sample liquid and air. In addition, this inlet 1
A pair of large diameter portions 18.18 are formed above and below 6.

On the other hand, the holder member 20 is a cylindrical member that can removably house the capsule member 10, and has an opening 2 at its lower end for protruding the dripping tip 22.
2, and a vent 24 is provided in the center.

When the capsule member 10 is attached to the holder member 20, the outer wall of the large diameter portion 18.18 of the capsule member 10 and the inner wall of the holder member are airtightly connected, and the injection port 16 and the ventilation port 24 are connected. It's starting to get through.

The material for forming the capsule member 10 and the holder member 20 is not particularly limited, and any material such as glass, plastic, metal, ceramics, etc. can be used. However, especially when the capsule member is made of transparent or translucent material, the contents (i.e. sample liquid)
This is preferable because the type and amount of liquid can be visually checked. Further, the capsule member 10 and the holder member 20 can be easily attached and detached, and when attached, the large diameter portion 18.
In order to obtain airtightness between the outer wall of 8 and the inner wall of the holder member, the capsule member 10 may be formed of a relatively hard material, and at least the inner wall portion of the holder member 20 may be formed of a relatively flexible material. preferable. For example, the outer wall of the holder member is made of hard plastic.

It may be made of glass, metal, ceramic, etc., and an elastic inner lining (lining or coating) made of rubber, synthetic rubber, soft plastic, etc. may be provided on the inner wall thereof. According to such a configuration, the elastic contact between the outer wall of the large diameter portion of the capsule member and the inner wall of the holder member makes the airtightness between them more reliable, and when the bottom of the capsule member and the bottom of the holder member come into contact, impact is alleviated.

It is also preferable to form the large diameter portion by fitting a ring made of soft and elastic rubber, synthetic rubber, plastic, etc. into the holder member.

When using such a sample liquid spotter to supply a sample liquid onto an analysis material, first remove the capsule member 10 from the holder member 20 and then apply the sample liquid from the injection port 16 with a syringe or the like. Inject into the capsule member 10. Alternatively, an empty capsule member 10 is inserted into the holder member 20 in advance, and the air inside the holder member 20 is sucked through the vent 24 to reduce the pressure inside the capsule member 10, so that the sample liquid is dripped at the lower end of the tip portion 12. From inlet 1
It is also possible to siphon it up to a portion below 6 and store it inside the capsule member 10.

Further, the portion of the upper end portion 14 of the capsule member 10 that does not come into contact with the holder member 10 when inserted into the holder member 20 may be configured to have a shape similar to a soft and elastic rubber bulb portion of a dropper ( For example, when the capsule member main body is made of rubber, plastic, etc., its thickness may be reduced; generally, the upper end is opened in a cylindrical shape, and a soft and elastic rubber, etc. (It may be covered with a hemispherical member made of synthetic rubber, polymer, etc.) In such a configuration, temporarily seal the injection port 16 with the pad of your finger or the like, and in the same way as operating a dropper, recess the spherical part at the upper end and insert the lower end of the dripping tip 12 into the sample liquid. The sample liquid may be sucked into the capsule member by soaking it and restoring the bulb.

The capsule member 10 into which an appropriate amount of sample liquid has been injected is inserted into the holder part 4420 with the dropping tip part 12 protruding from the opening 22. The holder member 20 with the capsule member 10 mounted thereon is arranged so that the lower end of the dropping tip portion 12 is positioned directly above the analysis material 30 to be used. Here, when an appropriate amount of air is supplied from the ventilation port 24 in the direction of the arrow in FIG.
A droplet of an appropriate size is formed at the lower end of the . Then,
When at least one of the spotter and the analysis material 30 is moved in the vertical direction to bring the interface of the droplet into contact with the surface of the analysis material 30, the droplet can be spot-supplied onto the analysis material 30.

After this, by placing the lower end of the dropping tip on another analysis material and introducing air through the vent 24, it is possible to easily and quickly spot-supply sample liquid to multiple analysis materials using the same operation. .

After the predetermined spotting is completed, the capsule member 10 can be removed from the holder part 120 and discarded while retaining the remaining sample inside. If a plurality of capsule members corresponding to one holder member are prepared, it is possible to easily and quickly analyze a plurality of types of sample liquids by replacing the capsule members.

[Brief explanation of the drawing]

FIGS. 1A and 1B respectively show -actual/l! of the present invention.
FIG. 2 is a perspective view not showing the capsule member and holder member constituting the sample liquid spotter according to the embodiment, and FIG. 2 is a side sectional view showing the sample liquid spot @sae when the capsule member is attached to the holder member. be. 10... Capsule member 12... Dripping tip portion 14... Upper end 16... Inlet port 1
8...Large diameter part 20...Holder member 2
2...Opening 24...Threads
Pore 30...Analysis materials No. 1A Diagrams Old ~ Old Figures ~ 22 12 Figure 1゜

Claims (1)

[Scope of Claims] (a) A cylindrical capsule member having an open lower end forming a dropping tip portion and a closed upper end, and holding a sample liquid for chemical analysis on the inner lower end side; ) A cylindrical holder member that removably houses the capsule member with the dropping tip protruding from the lower end, and a cylindrical holder member suitable for injecting the sample liquid and air into the center of the capsule member. An inlet is provided, and a pair of large diameter parts are formed above and below this injection port, and the outer walls of these large diameter parts are airtight with the inner wall of the holder member when the capsule member is stored in the holder member. The holder part has an air vent located between the pair of large diameter parts when the capsule member is housed, and which can send air to the injection port. A sample liquid spotter characterized by being provided with a spout.