JPS57150382A - Purification of super oxide dismutase - Google Patents

Purification of super oxide dismutase

Info

Publication number
JPS57150382A
JPS57150382A JP3542081A JP3542081A JPS57150382A JP S57150382 A JPS57150382 A JP S57150382A JP 3542081 A JP3542081 A JP 3542081A JP 3542081 A JP3542081 A JP 3542081A JP S57150382 A JPS57150382 A JP S57150382A
Authority
JP
Japan
Prior art keywords
super oxide
oxide dismutase
sod
collected
adsorbed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3542081A
Other languages
Japanese (ja)
Inventor
Keijiro Miyata
Hitoshi Aoki
Toshio Kuroda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WAKAMOTO SEIYAKU KK
Wakamoto Pharmaceutical Co Ltd
Original Assignee
WAKAMOTO SEIYAKU KK
Wakamoto Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WAKAMOTO SEIYAKU KK, Wakamoto Pharmaceutical Co Ltd filed Critical WAKAMOTO SEIYAKU KK
Priority to JP3542081A priority Critical patent/JPS57150382A/en
Publication of JPS57150382A publication Critical patent/JPS57150382A/en
Pending legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: In separating and purifying super oxide dismutase (SOD), to make a desalting treatment in the process unnecessary, and not to take a long time for SOD to be adsorbed on an adsorbent.
CONSTITUTION: A solution containing raw super oxide dismutase is brought into contact with a crosslinked agarose gel linked with a phenyl group, and adsorbed super oxide dismutase is eluted and collected. Raw SOD as a starting raw material is preliminarily purified as follows. Namely, fresh human or bovine blood is collected in the presence of a blood coagulation inhibitor such as sodium citrate, cooled, centrifuged, the plasma of the supernatant liquid is removed, the erythrocyte of the deposit is washed with sodium chloride injection 2W3 times and collected. The prepared substance is subjected to hemolysis by ultrasonic treatment, saponin treatment, etc. and purified with DEAE cellulose.
COPYRIGHT: (C)1982,JPO&Japio
JP3542081A 1981-03-13 1981-03-13 Purification of super oxide dismutase Pending JPS57150382A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3542081A JPS57150382A (en) 1981-03-13 1981-03-13 Purification of super oxide dismutase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3542081A JPS57150382A (en) 1981-03-13 1981-03-13 Purification of super oxide dismutase

Publications (1)

Publication Number Publication Date
JPS57150382A true JPS57150382A (en) 1982-09-17

Family

ID=12441375

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3542081A Pending JPS57150382A (en) 1981-03-13 1981-03-13 Purification of super oxide dismutase

Country Status (1)

Country Link
JP (1) JPS57150382A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006634A1 (en) * 1989-11-07 1991-05-16 Bio-Technology General Corp. Improved method for purification of recombinant copper/zinc (cu-zn) superoxide dismutase from bacteria or eucaryotic cells
EP0822412A1 (en) * 1996-07-30 1998-02-04 Horiba, Ltd. Immunoassay method
US6106778A (en) * 1997-09-27 2000-08-22 Horiba, Ltd. Blood cell count/immunoassay apparatus using whole blood
US7326579B2 (en) 1996-07-30 2008-02-05 Horiba, Ltd. Immunoassay method for lyzed whole blood

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006634A1 (en) * 1989-11-07 1991-05-16 Bio-Technology General Corp. Improved method for purification of recombinant copper/zinc (cu-zn) superoxide dismutase from bacteria or eucaryotic cells
EP0822412A1 (en) * 1996-07-30 1998-02-04 Horiba, Ltd. Immunoassay method
US7326579B2 (en) 1996-07-30 2008-02-05 Horiba, Ltd. Immunoassay method for lyzed whole blood
US6106778A (en) * 1997-09-27 2000-08-22 Horiba, Ltd. Blood cell count/immunoassay apparatus using whole blood

Similar Documents

Publication Publication Date Title
DE3379644D1 (en) Adsorbent and process for preparing the same
EP0156961A3 (en) Process for preparing highly purified, stroma-free and hepatitis-resisting human and animal haemoglobin solutions
ES525361A0 (en) PROCEDURE TO PREPARE A SAFE MEDICATION AGAINST HEPATITIS CONTAINING PROTEIN CALLED INACTIVATOR CL.
JPS57116018A (en) Production of blood coagulation factor
ATE25690T1 (en) PLASMA PROTEIN SOLUTION WITH ACTIVE COAGAGATION, PROCESS FOR ITS PREPARATION AND ITS USE IN THE TREATMENT OF DISORDERS OF THE HEMOSTASIS SYSTEM.
JPS5944286B2 (en) Purification method of bovine thrombin
JPS57150382A (en) Purification of super oxide dismutase
GR75669B (en)
JPS57134419A (en) Stable anticoagulant of blood
JPS5758629A (en) Separating and purifying method of colonization stimulating factor and callicrein in human urine
ES8504210A1 (en) Process for the preparation of globin from hemoglobin, and globin obtained in this process.
JPS5781829A (en) Urea adsorbing resin
JPS56125310A (en) Preparation of protein solution consisting essentially of lipoprotein of plasma with high specific gravity and serum albumin
JPS54122713A (en) Separation of leukocyte
JPS61275224A (en) Collagenase-inhibiting agent
DE3361689D1 (en) Process for the separation and purification of proteases and antiproteases of blood coagulation and of the protease-antiprotease complex
SU1042752A1 (en) Method of treating chronic urethritis
JPS5649394A (en) Preparation of blood coagulation factor 5, and determination of abnormal prothrombinase activity
JPS5599191A (en) Purification of kallikrein in human urine
JPS6012025B2 (en) Purification method of superoxide dismutase
JPS5632421A (en) Fractionation of plasma protein
JPS5795918A (en) Purification of immunoglobulin
JPS5513002A (en) Preparation of urokinase
JPS57108018A (en) Endotoxin-sensitive substance
JPS54122715A (en) Separation and purification of factor inhibiting mutagenesis of mutagenic agent from cabbage juice