JPS57150382A - Purification of super oxide dismutase - Google Patents
Purification of super oxide dismutaseInfo
- Publication number
- JPS57150382A JPS57150382A JP3542081A JP3542081A JPS57150382A JP S57150382 A JPS57150382 A JP S57150382A JP 3542081 A JP3542081 A JP 3542081A JP 3542081 A JP3542081 A JP 3542081A JP S57150382 A JPS57150382 A JP S57150382A
- Authority
- JP
- Japan
- Prior art keywords
- super oxide
- oxide dismutase
- sod
- collected
- adsorbed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: In separating and purifying super oxide dismutase (SOD), to make a desalting treatment in the process unnecessary, and not to take a long time for SOD to be adsorbed on an adsorbent.
CONSTITUTION: A solution containing raw super oxide dismutase is brought into contact with a crosslinked agarose gel linked with a phenyl group, and adsorbed super oxide dismutase is eluted and collected. Raw SOD as a starting raw material is preliminarily purified as follows. Namely, fresh human or bovine blood is collected in the presence of a blood coagulation inhibitor such as sodium citrate, cooled, centrifuged, the plasma of the supernatant liquid is removed, the erythrocyte of the deposit is washed with sodium chloride injection 2W3 times and collected. The prepared substance is subjected to hemolysis by ultrasonic treatment, saponin treatment, etc. and purified with DEAE cellulose.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3542081A JPS57150382A (en) | 1981-03-13 | 1981-03-13 | Purification of super oxide dismutase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3542081A JPS57150382A (en) | 1981-03-13 | 1981-03-13 | Purification of super oxide dismutase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57150382A true JPS57150382A (en) | 1982-09-17 |
Family
ID=12441375
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3542081A Pending JPS57150382A (en) | 1981-03-13 | 1981-03-13 | Purification of super oxide dismutase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57150382A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991006634A1 (en) * | 1989-11-07 | 1991-05-16 | Bio-Technology General Corp. | Improved method for purification of recombinant copper/zinc (cu-zn) superoxide dismutase from bacteria or eucaryotic cells |
EP0822412A1 (en) * | 1996-07-30 | 1998-02-04 | Horiba, Ltd. | Immunoassay method |
US6106778A (en) * | 1997-09-27 | 2000-08-22 | Horiba, Ltd. | Blood cell count/immunoassay apparatus using whole blood |
US7326579B2 (en) | 1996-07-30 | 2008-02-05 | Horiba, Ltd. | Immunoassay method for lyzed whole blood |
-
1981
- 1981-03-13 JP JP3542081A patent/JPS57150382A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991006634A1 (en) * | 1989-11-07 | 1991-05-16 | Bio-Technology General Corp. | Improved method for purification of recombinant copper/zinc (cu-zn) superoxide dismutase from bacteria or eucaryotic cells |
EP0822412A1 (en) * | 1996-07-30 | 1998-02-04 | Horiba, Ltd. | Immunoassay method |
US7326579B2 (en) | 1996-07-30 | 2008-02-05 | Horiba, Ltd. | Immunoassay method for lyzed whole blood |
US6106778A (en) * | 1997-09-27 | 2000-08-22 | Horiba, Ltd. | Blood cell count/immunoassay apparatus using whole blood |
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