JPS5599191A - Purification of kallikrein in human urine - Google Patents
Purification of kallikrein in human urineInfo
- Publication number
- JPS5599191A JPS5599191A JP595279A JP595279A JPS5599191A JP S5599191 A JPS5599191 A JP S5599191A JP 595279 A JP595279 A JP 595279A JP 595279 A JP595279 A JP 595279A JP S5599191 A JPS5599191 A JP S5599191A
- Authority
- JP
- Japan
- Prior art keywords
- kallikrein
- human urine
- purification
- sepharose
- arginine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To purify kallikrein (K) in human urine effectively, by treating a K-containing solution with arginine-Sepharose.
CONSTITUTION: Human urine or a crude solution contaning kallikrein (K) is adjusted to pH7.0W9.0, and brought into contact with arginine-Sepharose (A) adjusted to the same pH, preferably about 8.0, to have K adsorbed on A specifically. The adsorbed K is then eluted with a buffer of pH7.0W9.0 containing 0.1W0.5mol of sodium chloride.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP595279A JPS5599191A (en) | 1979-01-18 | 1979-01-18 | Purification of kallikrein in human urine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP595279A JPS5599191A (en) | 1979-01-18 | 1979-01-18 | Purification of kallikrein in human urine |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5599191A true JPS5599191A (en) | 1980-07-28 |
Family
ID=11625222
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP595279A Pending JPS5599191A (en) | 1979-01-18 | 1979-01-18 | Purification of kallikrein in human urine |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5599191A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0098123A2 (en) * | 1982-06-30 | 1984-01-11 | NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JAPAN CHEMICAL RESEARCH CO., LTD | A process for concentrating and purifying human urinary kallikrein |
US4780209A (en) * | 1984-06-06 | 1988-10-25 | Japan Chemical Research Co., Ltd. | Process for concentrating and separating trypsin inhibitor and kallidinogenase in human urine |
JP2006127180A (en) * | 2004-10-29 | 2006-05-18 | Nec Infrontia Corp | Pc card |
-
1979
- 1979-01-18 JP JP595279A patent/JPS5599191A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0098123A2 (en) * | 1982-06-30 | 1984-01-11 | NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JAPAN CHEMICAL RESEARCH CO., LTD | A process for concentrating and purifying human urinary kallikrein |
US4780209A (en) * | 1984-06-06 | 1988-10-25 | Japan Chemical Research Co., Ltd. | Process for concentrating and separating trypsin inhibitor and kallidinogenase in human urine |
JP2006127180A (en) * | 2004-10-29 | 2006-05-18 | Nec Infrontia Corp | Pc card |
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