JPS57141401A - Production of pullulan with narrowed molecular weight distribution - Google Patents

Production of pullulan with narrowed molecular weight distribution

Info

Publication number
JPS57141401A
JPS57141401A JP15613481A JP15613481A JPS57141401A JP S57141401 A JPS57141401 A JP S57141401A JP 15613481 A JP15613481 A JP 15613481A JP 15613481 A JP15613481 A JP 15613481A JP S57141401 A JPS57141401 A JP S57141401A
Authority
JP
Japan
Prior art keywords
pullulan
average
product
fractions
ratio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15613481A
Other languages
Japanese (ja)
Other versions
JPH0248561B2 (en
Inventor
Mikihiko Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hayashibara Seibutsu Kagaku Kenkyujo KK
Original Assignee
Hayashibara Seibutsu Kagaku Kenkyujo KK
Hayashibara Biochemical Laboratories Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hayashibara Seibutsu Kagaku Kenkyujo KK, Hayashibara Biochemical Laboratories Co Ltd filed Critical Hayashibara Seibutsu Kagaku Kenkyujo KK
Priority to JP15613481A priority Critical patent/JPS57141401A/en
Publication of JPS57141401A publication Critical patent/JPS57141401A/en
Publication of JPH0248561B2 publication Critical patent/JPH0248561B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

PURPOSE: To obtain high-purity pullulan with a narrowed MW distribution in high yields, by partially decomposing raw pullulan, fractionating the decomposition product and collecting the fractions having a weight-average MW/number- average MW ratio of below a specified value.
CONSTITUTION: Raw pullulan is partially decomposed, the decomposition product is fractionated and the fractions having a weight-average MW/number-average MW ratio of below 1.5 are collected. The resulting pullulan fractions ar usually decolorized and desalted by active carbon, an ion exchange resin, etc., to obtain the purpose pullulan product.
USE: This pullulan product is used advantageously as a water-soluble high-molecular standard reagent. For example, in case where it is used in gel filtration or liquid chromatography, it is suitable to use pullulan with a MW distribution which is narrowed to such an extent that the reversed Mw/reversed Mn ratio is about 1.3W1.1. Moreover, the product is useful as a plasma expander, a blood flow improver or the like.
COPYRIGHT: (C)1982,JPO&Japio
JP15613481A 1981-10-02 1981-10-02 Production of pullulan with narrowed molecular weight distribution Granted JPS57141401A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15613481A JPS57141401A (en) 1981-10-02 1981-10-02 Production of pullulan with narrowed molecular weight distribution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15613481A JPS57141401A (en) 1981-10-02 1981-10-02 Production of pullulan with narrowed molecular weight distribution

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP14295380A Division JPS5767602A (en) 1980-10-15 1980-10-15 Preparation of pullulan for expanding plasma

Publications (2)

Publication Number Publication Date
JPS57141401A true JPS57141401A (en) 1982-09-01
JPH0248561B2 JPH0248561B2 (en) 1990-10-25

Family

ID=15621060

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15613481A Granted JPS57141401A (en) 1981-10-02 1981-10-02 Production of pullulan with narrowed molecular weight distribution

Country Status (1)

Country Link
JP (1) JPS57141401A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518902A (en) * 1992-08-20 1996-05-21 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo High pullulan content product, and its preparation and uses
WO2002030485A1 (en) * 2000-10-10 2002-04-18 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Embolic materials
WO2004048420A1 (en) * 2002-11-22 2004-06-10 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo High-concentration liquid pullulan and method of circulating the same
CN103172757A (en) * 2012-12-31 2013-06-26 天津北洋百川生物技术有限公司 Extracting process of pulullan polysaccharide

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5767602A (en) * 1980-10-15 1982-04-24 Hayashibara Biochem Lab Inc Preparation of pullulan for expanding plasma

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5767602A (en) * 1980-10-15 1982-04-24 Hayashibara Biochem Lab Inc Preparation of pullulan for expanding plasma

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518902A (en) * 1992-08-20 1996-05-21 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo High pullulan content product, and its preparation and uses
WO2002030485A1 (en) * 2000-10-10 2002-04-18 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Embolic materials
US7414038B2 (en) 2000-10-10 2008-08-19 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Embolic materials
WO2004048420A1 (en) * 2002-11-22 2004-06-10 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo High-concentration liquid pullulan and method of circulating the same
JPWO2004048420A1 (en) * 2002-11-22 2006-03-23 株式会社林原生物化学研究所 High concentration liquid pullulan and its distribution method
CN103172757A (en) * 2012-12-31 2013-06-26 天津北洋百川生物技术有限公司 Extracting process of pulullan polysaccharide

Also Published As

Publication number Publication date
JPH0248561B2 (en) 1990-10-25

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