JPS56164797A - Improved method of determining components in samples - Google Patents

Improved method of determining components in samples

Info

Publication number
JPS56164797A
JPS56164797A JP6824580A JP6824580A JPS56164797A JP S56164797 A JPS56164797 A JP S56164797A JP 6824580 A JP6824580 A JP 6824580A JP 6824580 A JP6824580 A JP 6824580A JP S56164797 A JPS56164797 A JP S56164797A
Authority
JP
Japan
Prior art keywords
substance
sample
saccharose
glucose
converted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6824580A
Other languages
Japanese (ja)
Other versions
JPS6258719B2 (en
Inventor
Kunio Matsumoto
Rokuro Izumi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Jozo KK
Original Assignee
Toyo Jozo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Jozo KK filed Critical Toyo Jozo KK
Priority to JP6824580A priority Critical patent/JPS56164797A/en
Priority to FR8110136A priority patent/FR2483081A1/en
Priority to GB8115611A priority patent/GB2078369B/en
Publication of JPS56164797A publication Critical patent/JPS56164797A/en
Publication of JPS6258719B2 publication Critical patent/JPS6258719B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: One of components to be determined in a sample is converted into the second substance and the substance is determined using the oxidase corresponding to the second substance wherein the second substance in the sample is previously removed by phosphorylation, then the oxidase in made to act on, thus permitting the precise determination.
CONSTITUTION: In clinical biochemistry or in the field of fermentation, for example, when saccharose or lactose in a sample such as in blood or in a culture medium is determined, the saccharose or the like is converted into glucose (the second substance) and the oxidase corresponding to the second substance is used and the oxygen consumed or hydrogen peroxide formed are measured with an oxygen electrode, hydrogen peroxide electrode or enzyme electrode. At this time, the glucose previously existing in the sample is removed using immobilized kinase and adenosine triphosphate and the saccharose in the sample is converted into the glucose. Thus, the operations are simplified, the amount of the reagent used is lessened and the time for measurement is shortened and accurate results are obtained.
COPYRIGHT: (C)1981,JPO&Japio
JP6824580A 1980-05-21 1980-05-21 Improved method of determining components in samples Granted JPS56164797A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP6824580A JPS56164797A (en) 1980-05-21 1980-05-21 Improved method of determining components in samples
FR8110136A FR2483081A1 (en) 1980-05-21 1981-05-21 IMPROVEMENT TO A METHOD FOR DETERMINING A CONSTITUENT IN A SAMPLE
GB8115611A GB2078369B (en) 1980-05-21 1981-05-21 Enzymatic assay method and apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6824580A JPS56164797A (en) 1980-05-21 1980-05-21 Improved method of determining components in samples

Publications (2)

Publication Number Publication Date
JPS56164797A true JPS56164797A (en) 1981-12-17
JPS6258719B2 JPS6258719B2 (en) 1987-12-07

Family

ID=13368181

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6824580A Granted JPS56164797A (en) 1980-05-21 1980-05-21 Improved method of determining components in samples

Country Status (3)

Country Link
JP (1) JPS56164797A (en)
FR (1) FR2483081A1 (en)
GB (1) GB2078369B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011214912A (en) * 2010-03-31 2011-10-27 Cci Corp Biosensor

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59163555A (en) * 1983-03-08 1984-09-14 Oriental Yeast Co Ltd Quick analyzing method of plural components
US5175088A (en) * 1983-03-08 1992-12-29 Oriental Yeast Co. Ltd. Rapid analysis of plural components
CS238261B1 (en) * 1983-07-28 1985-11-13 Jiri Cerkasov Enzymatic reactions execution and tracing method and device for application of this method
DE3530076A1 (en) * 1985-08-22 1987-02-26 Max Planck Gesellschaft METHOD FOR DETECTING COMPOUNDS THAT CAN BE DELIVERED OR INCLUDED BY ENZYME ELECTRONES
DE3811099A1 (en) * 1988-03-31 1989-10-12 Biometra Biomedizinische Analy METHOD FOR DETECTING COMPOUNDS THAT CAN CATALYZE BY OXIDASE ELECTRONES
WO1997024456A1 (en) * 1995-12-27 1997-07-10 Asahi Kasei Kogyo Kabushiki Kaisha Method for assaying vital sample
GB0100770D0 (en) * 2001-01-11 2001-02-21 Celsis Internat Plc Hygiene monitoring

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT339505B (en) * 1974-03-14 1977-10-25 Boehringer Mannheim Gmbh ENZYMATIC ANALYSIS PROCEDURE
DK401279A (en) * 1978-09-26 1980-03-27 Toyo Jozo Kk MULTI-COMPONENT ANALYSIS METHOD AND APPARATUS FOR USE THEREOF
DE2913553C2 (en) * 1979-04-04 1981-09-17 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for the enzymatic determination of enzyme substrates

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011214912A (en) * 2010-03-31 2011-10-27 Cci Corp Biosensor

Also Published As

Publication number Publication date
FR2483081A1 (en) 1981-11-27
GB2078369B (en) 1983-06-22
FR2483081B1 (en) 1985-03-15
GB2078369A (en) 1982-01-06
JPS6258719B2 (en) 1987-12-07

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