JP4020843B2 - Analytical apparatus and reagent container - Google Patents

Description

The present invention relates to an automatic analyzer, and relates to an analyzer that analyzes a sample using a reagent.

  An automatic analyzer is a device that automatically analyzes components contained in a sample by reacting with a reagent or the like, and is disclosed in JP-A-9-127123. In a chemical analyzer, a user designates a plurality of analysis items for a plurality of samples and performs an analysis operation. One analysis operation involves dispensing of samples and reagents, stirring, and counting, etc. In reagent dispensing, multiple reagent bottles are placed on the turntable so that the target reagent can be moved to the dispensing position. It has become.

  In JP 2000-275251 A, in order to suppress the movement of the reagent bottle, the lateral acceleration is applied and the shaking of the reagent in the reagent bottle is increased, resulting in an increase in dispensing time and a splash of the reagent. The form is disclosed.

JP-A-9-127123

JP 2000-275251 A

  However, in the case of a high-speed analyzer in which the processing speed of the analyzer is improved and improved in order to increase the throughput of the analyzer, the form of the prior art is not sufficient. It is also necessary to increase the movement speed of the reagent bottle, and the acceleration during movement is also very large. Therefore, the splashing of the reagent from the reagent bottle and the stabilization time of the liquid level vibration are prolonged, the deterioration of the analysis accuracy is expected, and there is a limit to shortening the analysis time.

  Therefore, an object of the present invention is to provide an analyzer that can move reagent bottles at high speed and realize high-speed processing.

In order to solve the above problems, the present invention has the following aspects.
(1) Reagent holding part formed so that a plurality of reagent containers containing reagent liquids can be installed, a reaction container for reacting the reagent liquid and the sample to be analyzed, and the reagent liquid of the selected reagent holding part are taken out A reagent dispensing unit that leads to the reaction container; and a moving mechanism that moves the reagent container of the selected reagent solution to a position where the reagent container is taken out by the dispensing unit. A reagent outlet by the injection unit; and a cylindrical member inserted into the reagent outlet, the cylindrical member having an opening area below the liquid level smaller than an opening area on the liquid level. It is an analyzer characterized in that it is provided with a storage part for the formed reagent container. Or it is a reagent container used for these analyzers.

  In addition, it arrange | positions so that the said reagent dispensing unit at the time of the said reagent extraction may be inserted inside a cylindrical member.

  For example, the lower opening located in the liquid level of the said cylindrical member is a shape containing the edge | side of the bottom face of the said cylindrical member. Or the lower opening part of a cylindrical member is a shape containing the edge | side and side surface of the bottom face of the said cylinder.

  Providing a protrusion on the inner wall of the cylindrical member is preferable from the viewpoint of suppressing splashing and shaking. For example, it is preferable to provide a protrusion on the inner wall of the region located above the liquid level of the cylindrical member from the viewpoint of suppressing splashing and suppressing fluctuations in the liquid level. Alternatively, it is preferable to provide a protrusion on the inner wall of the region located below the liquid level from the viewpoint of suppressing the amount of liquid that varies greatly with container movement.

  For example, at least one or more thin donut-shaped protrusions can be provided on the inner side of the cylinder above the liquid level when the reagent container is fully filled.

In addition, a protrusion is provided inside the cylindrical member. For example, at least one partition wall having at least half the length of the cylinder can be provided. Alternatively, at least one auxiliary cylinder having at least half the length of the cylinder can be provided.
(2) Alternatively, a lid member that is arranged in the cylindrical member so as to change its position based on the liquid level position of the reagent solution, and that narrows the flow path of the reagent solution that is configured inside the cylindrical member; It is an analyzer characterized by including the reagent container storage section provided.
Or it is a reagent container used for this.

For example, the lid member is made smaller than the inner cross-sectional area of the cylinder. Further, the lid member is made of a material smaller than the specific gravity of the reagent. The lid member is provided inside the cylinder so as to be vertically movable.

  The lid member is formed to have an opening and an upper surface portion around the opening. For example, it is preferable that the opening formed in the lid member is provided with a concentric circular through hole larger than the outer diameter when a reagent extraction part such as a probe is inserted. The second region closer to the opening than the first region of the upper surface portion is formed to be closer to the reagent liquid surface than the first region of the upper surface portion. preferable. For example, in the lid member, the upper part of the lid such as the upper surface of the lid is formed into a mortar shape, and the through hole as the opening is provided in the bottom part of the mortar.

  More preferably, an air hole through which air passes is provided in the side wall surface of the cylindrical member below the upper wall surface of the reagent container.

  Further, a reagent filling port different from the port into which the dispensing unit of the reagent container is introduced can be provided. It is preferable to provide this mouth in the upper part in the vertical direction of the antinode of the liquid surface wave.

  By adopting the configuration as described above, it is possible to provide an analyzer capable of moving reagent bottles at high speed and realizing high-speed processing.

  Thereby, the number of analyzes per unit time can be improved.

  Moreover, a reagent container suitable for an analyzer equipped with a high-speed reagent moving mechanism can be obtained.

  For example, the reagent bottle can be moved at high speed, and the number of analyzes per unit time can be improved. In addition, the usage rate of the reagent injected into the reagent bottle can be improved. Moreover, the amount of evaporation of the reagent can be reduced by reducing the contact area between the reagent and the outside air.

  According to the present invention, it is possible to provide an analyzer capable of moving reagent bottles at high speed and realizing high-speed processing.

  Examples of the invention are described below. In addition, this invention is not limited to the content as described in this specification, and does not inhibit the deformation | transformation based on a well-known technique.

  FIG. 1 is a perspective view showing an example of the configuration of the automatic analyzer of this embodiment, FIG. 2A is an example of a reagent container equipped in the automatic analyzer shown in FIG. 1, and FIG. It is sectional drawing of the reagent container with which the automatic analyzer shown to Fig.2 (a) is equipped.

  1 includes a sample cup 101 containing a sample, a sample disk 102 storing a plurality of sample cups, a reagent container 103 storing a reagent, a reagent disk 104 storing the plurality of reagent containers, a sample and Vessel 105 that dispenses reagents to cause reaction, incubator 106 that stores the plurality of vessels, sample dispensing unit 107 that dispenses samples, reagent dispensing unit 108 that dispenses reagents, and agitation of sample and reagents A stirring unit 113 that measures the concentration of the object, and a cleaning unit 109 that cleans the vessel 105.

  The sample dispensing unit 107 and the reagent dispensing unit include a probe that takes in the reagent / sample and discharges it to the vessel.

  As a flow of analysis operation of the automatic analyzer, first, a reagent to be mixed with a sample arranged on the sample disk 102 is determined. Then, the sample disk is rotated so that the target sample cup 101 is at a predetermined sorting position. Next, the sample is dispensed into the vessel 105 by the sample dispensing unit 107. Furthermore, in this figure, the incubator 106 rotates clockwise by one vessel, and the other samples are sequentially dispensed to the adjacent vessel 105. When the sample reaches the reagent dispensing unit 108 to the dispensing position, the reagent is immediately dispensed by the reagent dispensing unit 108 into the vessel 105 containing the sample. The reagent to be dispensed at this time is selected according to the target sample, and the reagent disk 104 is rotated so that the selected reagent comes to the dispensing position of the reagent dispensing unit 108, and the reagent dispensing is performed. It is to be sorted in advance by the note unit 108. Next, the sample and the reagent react with each other by the stirring unit 113, and the result is measured by the measuring unit 110. A series of operations at this time is controlled by the controller 111, and the measured result is sent to the information terminal 112 and displayed as an analysis result.

  A plurality of reagent containers are arranged on a table, and when the table moves, the target reagent container is formed so as to move under the target probe, and a probe having an outer diameter equal to or smaller than the reagent container outlet inner diameter is provided as a reagent container. Inserted into.

  At this time, in order to increase the number of analyzes per unit time, in order to speed up this series of analysis operations, it is important to speed up reagent dispensing. Various types of reagents are prepared on the reagent disk 104, but there are also various reagents that are instructed to the sample. If the reagent disk is rotated at high speed to prepare for dispensing by the reagent dispensing unit 108, Don't be. However, as the rotational speed increases, the liquid level in the reagent container 103 greatly vibrates due to centrifugal force and acceleration, and the reagent may splash outside the reagent container 103 during the movement. Even after stopping, the vibration of the liquid level remains large. If the automatic analyzer continues to operate in this state, the reagent probe will be inserted into an unstable liquid surface, air contamination due to insufficient insertion distance, an increase in the amount of contamination on the outer wall of the reagent probe due to excessive insertion distance, This will increase the reagent probe movement time.

  In this apparatus, for example, in a chemical analyzer, a user designates a plurality of analysis items for a plurality of samples. Then, it can have the function to process automatically the process of the analysis operation which consists of these. One analytical operation is performed by a combination of basic operations such as sample and reagent dispensing, agitation, and counting. This chemical analyzer is equipped with units that perform these basic operations, and each unit is sequence-controlled by a computer to realize one analytical operation. Therefore, in these operations, in reagent dispensing, a plurality of reagent bottles are arranged on the turntable, and the target reagent moves to the dispensing position.

  Therefore, in this embodiment, the chimney 201 is provided in the reagent container 103 in order to suppress the liquid level vibration and to stabilize the liquid level early when the movement is stopped. A cylindrical chimney 201 is provided at the outlet of the reagent container 103 (reagent extraction (intake) port into which the probe is introduced). Two outlines are shown in the figure. The inside of the chimney 201 communicates with the atmosphere at the top, and has an upper opening for introducing the probe, and a lower opening that communicates with the surrounding reagents at the bottom. The outer wall of the chimney 201 is in close contact with the outlet of the reagent container 103. Yes. The reagent can freely come and go using this lower opening. The reagent can freely come and go using this lower opening, but the flow of the reagent in the chimney is restricted by the fluid resistance by the chimney. In the figure, the apex side on the triangle away from the opening is set to be the center side of the rotating reagent disk 104. The reagent container 103 has a tapered bottom like a mortar and is formed so that the vicinity of the chimney 201 is lower than other regions. In addition, the bottom of the chimney is one step down. For this reason, even when the amount of the reagent is reduced, it is easy to gather at the lower part of the reagent probe, and the collected reagent becomes difficult to move, so that the stabilization waiting time at the time of inhalation can be shortened. Further, by providing at least one air hole 203 through which the air passes through or below the upper wall surface of the reagent container 103 and on the side wall surface of the chimney, or by making a cut above the position, the upper space of the reagent container 103 can be reduced. Connected to the atmosphere. This air hole 203 is provided in order to send air into the reagent container 103 when the reagent in the chimney is separated by the probe, and to prevent a constant drop in the liquid level in the chimney with respect to the liquid level in the reagent container 103. is there. In addition, since the liquid can pass through the air holes, the liquid vibration mode of the container shape is examined to prevent the liquid from passing through the air holes when the liquid level vibrates. It is desirable to fix the hole orientation in the direction.

Further, the opening area of the lower opening is smaller than that of the upper opening. The smaller the opening area of the lower opening, the greater the resistance against the flow of liquid inside and outside the chimney. For this reason, even if the liquid level in the reagent container has a large amplitude due to the acceleration from the outside to the reagent container 103, the vertical speed of the liquid level in the chimney can be greatly reduced. For this reason, the splash of the reagent from the reagent container 103 can be reduced, and the liquid surface stabilization waiting time when dispensing with the reagent probe can be greatly reduced.
The opening area of the lower opening portion needs to be 0.1 to 100 square millimeters, preferably 0.5 to 50 square millimeters, and more preferably 1 to 30 square millimeters. It is preferable that the area of the opening formed below the liquid surface is sufficiently large to be at least about 0.5% with respect to the area of the opening formed on the liquid surface of the reagent. On the other hand, it is preferably about 80% or less from the viewpoint of liquid level stabilization.

  In addition, the smaller the opening, the lower the vertical speed of the reagent liquid surface, but on the other hand, the time required for refilling the reagent increases. However, it is possible to reduce the time required for refilling by separately installing a filling port for refilling in the reagent container 103, and it is possible to prevent scattering of the reagent by installing a sealing lid.

  In addition, since it is necessary to dispense the liquid in the reagent container 103 with the liquid level stabilized as much as possible, it is desirable to install the lower opening of the chimney on the lowest surface of the chimney. However, when a chimney with an opening on the bottom is inserted into the reagent container, if it is inserted to the bottom, the lower opening will be blocked. You may prevent the lower opening part from being closed by contact.

  In addition, a probe for dispensing a reagent is inserted into the chimney. The probe is provided with a sensor for measuring the liquid level. If this sensor is of the capacitive type, it may malfunction if it comes into contact with the reagent attached to the chimney during insertion, and the liquid level may not be measured accurately. For this reason, it is desirable to make the inner wall of the chimney water-repellent or to increase the inner diameter of the chimney to make it difficult to contact the droplets.

  Furthermore, it is desirable that the position of the outlet of the reagent container 103 is at the upper part of the standing wave node of the liquid level vibration in both the r and θ directions in FIG. This is because the stabilization waiting time at the time of dispensing can be reduced due to less vertical movement at the node position. If the reagent container 103 is a rectangular parallelepiped, it may be at the center of the distance in the vertical and horizontal directions. For example, in the case of a triangular prism as shown in FIG. 2, in the vibration in the r direction, the vibration node is slightly triangular than the center of the distance. The base side is a node.

  Further, when acceleration is applied to the reagent container 103, it is prevented from rising along the inner wall surface of the chimney and being scattered outside. For this reason, as shown in FIG. 2B, a protruding portion protruding inward from the chimney wall surface is provided. In the figure, an inward washer-like projection 202 having an opening at the center is provided. As a result, it is possible to effectively prevent the reagent from rising and scattering during control such as stopping the high-speed movement of the reagent container.

  In addition, it is preferable that a reagent container bottom part is a taper shape which falls below from the circumference | surroundings toward the said exit right below. For example, the reagent container has a bottom portion directly below the outlet that is lower than the other bottom portions in a range of 4 square centimeters or less.

  FIG. 3 shows a second embodiment of the present invention. 1 and 2 can be used basically, but the chimney shape is characterized in that an opening is provided on the chimney side wall. Specifically, when the chimney is inserted, the chimney is formed in contact with the bottom surface, and the shape of the lower opening of the chimney 201 is formed to have a shape having an opening on the side wall including the bottom surface as shown in FIG. With this shape, the opening is not blocked and the reagent can freely come and go. Alternatively, from another viewpoint, there may be no opening on the bottom surface side, or the side having a large opening area may be on the side wall side.

FIG. 4 shows a third embodiment of the present invention. Is basically can be used in the form 1 and 2, in this embodiment, the chimney - position in response to changes in the liquid level is provided a member changes. Specifically, FIG. 4A is a cross-sectional view , and FIG. 4B is a perspective view. In this embodiment, the protrusion 202 shown in FIG. 2 is movable. A floating lid 210 is provided in the chimney that changes its position based on the liquid level position of the reagent liquid and narrows the flow path of the reagent liquid formed inside the chimney. By installing the floating lid 210 with an object having a specific gravity smaller than that of the reagent, it is possible to effectively prevent the splash of the reagent from moving up and down with the liquid surface. Further, when the reagent is refilled, it moves downward according to the injection amount of the reagent. Therefore, compared to the case where the protrusion 202 is fixed to the upper part, it is less likely to overflow, so that the reagent filling operation becomes easy. It is preferable to form cormorants by ensuring a space for inserting a probe into case of installing the floating lid 210. For example, by forming the opening and the upper surface of the floating lid 210 around the opening, it is possible to effectively insert and remove the probe while suppressing the floating lid 210 from being inserted at the position where the probe is inserted.

  FIG. 5 shows a fourth embodiment of the present invention. FIG. 5 (a) is a cross-sectional view, and FIG. 5 (b) is a perspective view. The floating lid 210 is formed so that its height decreases toward the end. For example, the upper part of the floating lid 210 having an opening is formed into a mortar shape toward the opening so as to provide an effect of guiding to the center when the probe is lowered. Alternatively, a disk-shaped floating lid 210 having no through hole at the center may be used. At this time, when the reagent is inhaled by the reagent probe, it is only necessary to inhale the reagent that rises from the surroundings by pressing down the lid and sinking or tilting.

  In addition, these anti-scattering lids not only prevent the purpose of anti-scattering but also reduce the contact area with the outside air, thereby preventing reagent evaporation and contributing to stabilization of the reagent concentration.

  FIG. 6 shows a fifth embodiment of the present invention. FIG. 6 (a) is a cross-sectional view, and FIG. 6 (b) is a perspective view. In this embodiment, a slit 211 is provided in the chimney 201. By installing at least one plate or cylinder in the chimney 201 in parallel or in a lattice and concentric circles, it becomes possible to increase the frictional resistance of the liquid, and the vertical movement of the liquid surface in the chimney 201 can be increased in the reagent container 103. It is possible to make the speed lower and lower than the amplitude of.

  Next, an embodiment of the drive pattern of the present invention is shown. By changing the drive pattern applied to the reagent container 103, the shaking of the reagent can be suppressed. The conventional drive pattern accelerates at a constant acceleration, maintains the speed, and decelerates at a constant acceleration, and leads to the destination with a trapezoidal speed pattern. In the embodiment of the present invention, the acceleration / deceleration time for giving acceleration is optimized.

A plurality of reagent containers is arranged on the table, by pre-Symbol table is moved, in the automatic analyzer reagent container object moves under the purpose of the probe, the acceleration-constant speed and deceleration of the reagent container drive pattern Is determined by the amount of liquid in the reagent container and the distance to the target position.

  Further, in the acceleration and deceleration of the drive pattern, it is preferable to drive the acceleration divided into a plurality of times.

  For example, when the reagent container of the selected reagent is moved to the dispensing position of the dispensing unit, it is controlled to accelerate by applying acceleration several times at intervals. After the first acceleration is applied at the time of movement, the acceleration is temporarily decreased from the first acceleration to be lower than the first acceleration, or a constant speed state is formed, and then the acceleration is further controlled by applying further acceleration. Similarly, at the time of deceleration, after the deceleration of the first acceleration is applied, the control is performed such that it is once loosened and then accelerated again to decelerate.

  In addition, in the acceleration and deceleration of the driving pattern, it is preferable that the absolute value of the acceleration is proportionally increased with time to half the target speed and then is decreased proportionally with time.

  In addition, in the acceleration and deceleration of this drive pattern, an integral multiple of the lowest frequency at which the excitation frequency component determined by the time at which the acceleration is applied is minimized, and the natural frequency in the direction of excitation of the liquid level is 30% error. It is preferable to match within the range.

  Further, at the time of acceleration or deceleration of the drive pattern, it is preferable to accelerate or decelerate using two types of acceleration. Alternatively, it is preferable that the acceleration is divided into two or more times when the driving pattern is accelerated or decelerated.

The natural frequency f _n of the standing wave generated on the liquid surface when the liquid is vibrated is

It becomes. Where T ₁ is the surface tension of the liquid (N / m), ρ is the density of the liquid (kg / m 3), l is the length in the vibration direction, and h is the depth. Therefore, the surface tension and density of the reagent are physical properties, and the length in the vibration direction becomes the shape of the reagent container 103 and thus becomes known. Further, the depth of the reagent can be grasped because it is measured every time by the reagent probe at the time of dispensing. For this reason, it is possible to grasp the natural frequency for each reagent container 103.

  Further, when a certain acceleration is applied to an object, the excitation frequency component changes according to the time given as shown in FIG. There are always multiple excitation components that minimize the excitation force at any acceleration application time.

Therefore, if the acceleration / deceleration time is adjusted so that the integral multiple of this minimum excitation component matches the natural frequency of the reagent, the amplitude of the liquid level after stopping the reagent container 103 is compared with the case where it is not adjusted. Can be greatly reduced. Specifically, for example, the natural frequency f ₁ is determined from the shape of the reagent container and the liquid surface position, and if this is 5 Hz, the acceleration time is set to 0.2, 0.4 so that the minimum value of the excitation component is 5 Hz. , 0.6 seconds, etc. In general, the frequency of the liquid surface in the reagent container is about 3 to 10 Hz, so it is better to set the acceleration application time to about 0.1 to 0.4 seconds so that the minimum value of the excitation component can take this value. . Also, the frequency identity for stabilizing the liquid level should be within 20% error range, preferably 5% or less, more preferably 3% or less.

  At this time, in most cases the target travel time is specified, and in order to achieve this, the acceleration is decreased or increased according to the increase / decrease of the acceleration time, and the maximum travel speed is made as uniform as possible. There is a need. Further, although the moving time varies depending on the moving distance, in this case, it may be dealt with by changing the constant speed moving time or by changing the acceleration.

  In addition, when the water level is low or the reagent container is large, the natural frequency of the liquid level becomes small. On the other hand, in order to reduce the minimum minimum value of the vibration component, it is necessary to increase the acceleration time. For example, if the minimum value of the vibration component required for liquid level stabilization is 3 Hz, the acceleration time must be 0.33 seconds. In this case, adding the deceleration time, it takes 0.67 seconds to stabilize the liquid level. If you want to shorten the time to less than 0.67 seconds for high-speed operation of the automatic analyzer, you can solve it by giving acceleration twice or more during acceleration / deceleration. For example, when acceleration is first applied and constant speed operation is performed, the liquid level generally vibrates while being attenuated for several seconds. At this time, the liquid level can be stabilized by giving an appropriate acceleration at the timing of eliminating this vibration. Similarly, even during deceleration, it is possible to suppress the vibration of the liquid level in the reagent container when the reagent disk is stopped by dividing the acceleration. Furthermore, the liquid level can be stabilized by accelerating / decelerating the acceleration using two or more values including negative values.

  In addition, various reagent containers with different reagent amounts and container shapes may be mounted on the reagent disk. For this reason, there are cases where the amount of reagent in the other reagent container 103, which is not the target of liquid level stabilization, is larger, or the shape is more susceptible to vibration. For this reason, scattering of liquid due to centrifugal force may occur not only in the target reagent container 103 but also in other reagent containers 103 mounted on the reagent disk 104. Therefore, it is desirable to determine the upper limit of the acceleration and speed of the reagent disk 104 in consideration of not only the reagent container 103 to be dispensed but also all the reagent containers 103 mounted on the reagent disk 104.

  According to the present invention described above, in an automatic analyzer, reagent bottles can be moved at high speed, and the number of analyzes per unit time can be improved. Furthermore, the usage rate of the reagent injected into the reagent bottle can be improved. Moreover, the amount of evaporation of the reagent can be reduced by reducing the contact area between the reagent and the outside air.

  In addition, in the said description, it is more preferable to implement about the above-mentioned reagent container and the automatic analyzer provided with this reagent container. However, in some cases, the present invention can be applied to an analyzer having a mechanism for moving a predetermined sample container to a dispensing position for dispensing a plurality of sample containers or sample containers containing a sample liquid. Alternatively, it may be applied to an apparatus including a plurality of liquid containers that hold other liquids and a mechanism that includes a plurality of liquid containers and moves them to a liquid take-out position.

The schematic diagram of one Example of the automatic analyzer of this invention. The schematic diagram of one Example of the automatic analyzer of this invention. The schematic diagram of one Example of the automatic analyzer of this invention. The schematic diagram of one Example of the automatic analyzer of this invention. The schematic diagram of one Example of the automatic analyzer of this invention. The schematic diagram of one Example of the automatic analyzer of this invention. An excitation frequency component generated when the automatic analyzer of the present invention is driven.

Explanation of symbols

102 ... Sample disc, 104 ... Reagent disc, 106 ... Incubator, 201 ... Chimney

Claims (4)

A reagent holding part formed so that a plurality of reagent containers containing the reagent liquid can be installed, a reaction container for reacting the reagent liquid and the sample to be analyzed, a reagent liquid in the selected reagent holding part is taken out, and the reaction is performed A reagent dispensing unit that leads to a container, and a moving mechanism that moves a reagent container of the selected reagent solution to a position where the reagent container is taken out by the dispensing unit,
The reagent holding portion is provided with a takeout reagent that by the dispensing unit, a tubular member which is inserted into the reagent outlet, and
In the tubular member,
A lid member that is arranged to change the position based on the liquid level position of the reagent solution and narrows the flow path of the reagent solution that is configured inside the cylindrical member, or a protrusion on the inner wall of the cylindrical member
Analyzer including the reagent container, characterized in that provision. A reagent holding part formed so as to be provided with a plurality of reagent containers for storing reagent liquids, a reaction container for reacting the reagent liquid and the sample to be analyzed, a reagent liquid in the selected reagent holding part are taken out, and the reaction is performed A reagent dispensing unit that leads to a container, and a moving mechanism that moves a reagent container of the selected reagent to a position where the reagent container is taken out by the dispensing unit . A reagent container,
The reagent container comprises a reagent outlet by the dispensing unit, and a cylindrical member inserted into the reagent outlet,
In the tubular member,
A lid member that is arranged to change the position based on the liquid level position of the reagent liquid and that narrows the flow path of the reagent liquid that is configured inside the cylindrical member, or positioned above the liquid level of the cylindrical member A reagent container, characterized in that a protrusion is provided on the inner wall of the region to be operated. 3. The reagent container according to claim 2, wherein the lid member is formed to have an opening and an upper surface portion around the opening. 4. The method according to claim 3, wherein the second region closer to the opening is closer to the reagent liquid surface than the first region of the upper surface portion than the first region of the upper surface portion. Reagent container.

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