JP2017128560A - Keratotic plug decomposition accelerator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a more effective keratotic plug decomposition accelerator that comprises a crude drug ingredient and hardly causes skin problems.SOLUTION: According to the present invention, a keratotic plug decomposition accelerator comprises a bottle gourd extract. The bottle gourd extract promotes the activities of an enzyme for keratotic plug decomposition. The enzyme is Cathepsin V, which is a cysteine protease whose localization in the keratotic plug has been revealed by the protein analysis of keratotic plug. The activities of the enzyme exert advantageous effects on, for example, the acceleration of decomposition of horn plug, the suppression of formation of horn plug, and the improvement of conspicuity of the pores. The present invention can be applied to medicines, quasi-drugs, cosmetics, foods, etc.SELECTED DRAWING: None

Description

  The present invention relates to an agent for accelerating the decomposition of horn plugs, which contains a Yugao extract.

  A keratin plug is thought to be a result of the sebum secreted from the surrounding keratin and sebaceous glands coagulating and developing in the pores, and is considered to be one of the causes of conspicuous pores. For this reason, treatments such as physically removing the square plugs have been proposed, such as a method of removing the face plugs by washing the face (Patent Document 1), a method of attaching the square plugs to an adhesive sheet or the like (Patent Document 2), A removal tool (Patent Document 3) has been devised. In addition, as an alternative method, a method and a preparation (Patent Document 4) for suppressing horn plug regeneration by adding a surfactant to a high-concentration organic acid have been shown.

  Yugao is used as edible as camphor, but it is also used as a cosmetic raw material. Its main patent documents are cosmetics having an antioxidant action (Patent Document 5), cosmetics having a whitening action (Patent Documents) 6, 7), cosmetics having an effect of promoting collagen production (Patent Document 8), hair restorers (Patent Document 9), and cosmetics having an effect of improving dry skin and giving skin elasticity (Patent Document 10). It is done.

  Pomegranate contains antioxidant components such as ellagic acid and can be expected to have an anti-aging effect, and it contains female hormone-like components that can be expected to have an effect on beauty and health. Used as a health food and cosmetic ingredient. In addition, pomegranate lactic acid fermentation broth has been shown to suppress the formation of inner root sheaths involved in the formation of horn plugs and to suppress the activation of male hormones, and to prevent the appearance of horn plugs and pores prophylactically. (Patent Document 11).

JP2007-119394A JP2007-55933A JP2009-82376 JP 2013-49667 A JP2002-138028 JP2014-55121A JP2014-224081 JP 2005-255527 A JP-A-6-247832 JP 2001-226249 A JP2015-168628

  In the method of removing keratin plugs with the pressure-sensitive adhesive sheets and instruments that have been developed so far, the horny skin of the epidermis is also lost, which may cause rough skin. In addition, the method of inhibiting the regeneration of horn plugs by adding a surfactant to a high concentration organic acid is based on the chemical dissolution of horn plugs, and it can be said that the concern of causing rough skin has been wiped out. Absent. This is due to the fact that the details of the mechanisms involved in the formation and degradation of horn plugs are unclear, and there are few studies focusing on the components of horn plugs. Recently, it has been revealed that about 70% of horn plugs are composed of proteins (Mizukoshi Koji et al., J. Soc. Cosmet. Chem. Jpn. 41, 262-268 (2007)). Was considered an important factor. Furthermore, protein analysis of horn plugs reported that keratin 17 was present in horn plugs (Hiroko Yamazaki et al., Abstracts of the 73rd SCCJ Research Conference p14-15 held on November 29, 2013) Not all the proteins that make up horn plugs have been clarified, and there are still many unclear points about the horn plug formation mechanism, horn plug decomposition mechanism, and constituent proteins. In view of the above background, in order to remove keratin plugs without causing rough skin, development of a new keratin plug decomposition accelerator based on the constituent protein in the keratin plug has been desired.

  In view of this situation, an object of the present invention is to provide a herbal component-containing keratolysis accelerator and a cathepsin V activity promoter based on a keratolysis mechanism.

  The present inventors have clarified that cathepsin V, which is a proteolytic enzyme, is localized inside the horn plug from the analysis of the protein in the hair follicle. Moreover, the cathepsin V enzyme activity in the horn plug was higher as the horn plug was smaller. Furthermore, the amount of corneodesmosine and the amount of cathepsin V, which are cell adhesion factors in horn plugs, have an inverse correlation, and the more conspicuous pores and horn plugs, the more corneodesmosine and the lower the amount of cathepsin V. If not, this relationship was reversed. Under such circumstances, cathepsin V is a horn plug-degrading enzyme, and as a result of intensive research that the promotion of the activity of cathepsin V can be a means of promoting the decomposition of the horn plug, Yugao extract has the activity of cathepsin V. It has been found that it has an accelerating action and an action of accelerating the decomposition of horn plugs. Furthermore, it discovered that a synergistic effect was exhibited with respect to the improvement of the conspicuousness of a pore by using together a Yugao extract and a pomegranate extract, and came to complete this invention.

  That is, the present invention is as follows.

  Cathepsin V activity promoter characterized by containing a Yugao extract.

  An agent for promoting the degradation of horn plugs, which contains Yugao extract.

  An agent for improving the conspicuous pores, characterized by containing a Yugao extract.

  An agent for improving pore conspicuousness, comprising a Yugao extract and a pomegranate extract.

  The Yugao extract of the present invention is a natural and highly safe material, and since it has recognized excellent cathepsin V activity promoting effect, it can be expected to improve the conspicuous improvement of pores by decomposing protein in the horn plug, Applications to pharmaceuticals, quasi drugs, cosmetics, foods, etc. are possible. The Yugao extract of the present invention has an action to promote keratoplug decomposition and can be used in the fields of pharmaceuticals, quasi drugs, cosmetics, and foods.

  Hereinafter, the present invention will be described in more detail.

  The Yugao extract used in the present invention is an extract of Yugao fruit. Yugao is a cucurbitaceae, genus Yugao, Yugao, and has the scientific name Lagenaria siceraria (L. ciceraria stand., L. ciceraria var. Hispida. The fruit may include pulp, pericarp, seeds, or pomace. In the extract of the fruit of Yugao which can be used in the present invention, examples of the solvent to be extracted include use of water, alcohols and acetone. What was extracted using the 1 type, or 2 or more types of mixed solvent of these water-soluble solvents may be used. Moreover, what was heat-extracted may be sufficient and what was extracted at normal temperature may be sufficient.

  The Yugao extract may be used as it is, and can be used as a cosmetic raw material after being subjected to treatments such as extraction, concentration, dilution and filtration, and decolorization and deodorization treatment with activated carbon or the like as necessary.

  The pomegranate extract used in the present invention is a part of a plant body such as a pomegranate (Punica granatum), a fruit, a seed, a stem, a leaf, a root or the like, an extract from whole plant, a fruit juice, or a fermentation of an extract or a fruit juice. It is a liquid. The extraction method is not particularly limited, and for example, it may be a heat extraction or a room temperature extraction. Preferably it is fruit juice, which may include pulp, pericarp, seeds, or pomace. As for the fruit juice, unadjusted or concentrated fruit juice may be used as it is, or it may be diluted. Moreover, you may adjust and adjust pH to 3-8 with alkalis, such as sodium hydroxide. More preferably, for the purpose of promoting the growth of Lactobacillus, organic substances such as yeast extract and soybean peptide, and minerals such as magnesium sulfate may be added. The fruit juice of the present invention includes a pomegranate solvent extract extracted with a solvent such as water or ethanol.

  In the fermentation liquid, for example, lactic acid bacteria can be used, and the lactic acid bacteria used belong to the genus Lactobacillus, Lactococcus, Leuconostoc, or Pediococcus. Examples include lactic acid bacteria. Particularly preferably, even if it is a lactic acid bacterium belonging to Lactobacillus plantarum in terms of improving the effectiveness by fermentation, particularly a strain isolated from plants or pickles that are fermented food thereof, It may be registered in. Moreover, what can ferment pomegranate juice efficiently in the range of pH 3-5 is especially preferable. The culture conditions can be set as appropriate, and it is preferable to carry out the culture at 25 to 40 ° C. as a guide.

  The above-mentioned extract may be used as it is for application to the external preparation or internal preparation of the present invention, and is used for cosmetics, quasi-drugs, pharmaceuticals, foods, etc. within the range not impairing the effect of the extract. Component fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickening Components such as an agent, a dye, an antioxidant, a whitening agent, a chelating agent, an excipient, a film agent, a sweetener, and a sour agent can be appropriately contained.

  Examples of the dosage form of the present invention include lotions, creams, massage creams, emulsions, gels, aerosols, packs, cleaning agents, bath preparations, foundations, powders, lipsticks, ointments, poultices, pastes, plasters. Essences, powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.), tablets, A drink etc. are mentioned.

  The content of the Yugao extract used in the present invention is not particularly limited, but is 0.00001% by weight or more, preferably 0.001 to 5.0% by weight in terms of solid matter. . If it is less than 0.00001% by weight, the effects of the present invention may not be sufficiently exerted. The addition method may be added in advance or may be added during production, and may be appropriately selected in consideration of workability.

  The content of the pomegranate extract used in the present invention is not particularly limited, but is 0.001% by weight or more, preferably 0.001 to 5.0% by weight in terms of solid matter. . If it is less than 0.001% by weight, the effects of the present invention may not be sufficiently exerted. The addition method may be added in advance or may be added during production, and may be appropriately selected in consideration of workability.

  Cathepsin V in the present invention is a proteolytic enzyme belonging to cysteine protease, and is sometimes referred to as cathepsin L2. It is expressed in skin epidermal cells, inner root sheaths in hair follicles, etc. and has been identified as an enzyme involved in keratin desquamation, and is thought to have an action of cleaving adhesion molecules between keratinocytes ( D. Bernard, et al. J. Invest. Dermatol. 120, 592-600 (2003), and Patrick L. J. M. Zeewen, et al. J. Invest. Dermatol. 127, 592-600 (2007)). . It has also been shown to be localized in intracellular lysosomes and involved in proteolysis (Y. Miwa, et al. FEBS Lett. 586, 3601-3607 (2012)). The relationship between skin color and cathepsin V has been pointed out, and application to whitening cosmetics and the like is expected (N. Chen, et al. J. Invest. Dermatol. 126, 2345-2347 (2006)). ). Furthermore, cathepsin V is expressed in cancer cells (I. Santamaria, et al. Cancer Res. 58, 1624-1630 (1998)) and is considered to be involved in the progression of cancer pathological conditions such as cancer metastasis. Therefore, application to medical treatment such as cancer diagnosis and treatment is expected.

Next, in order to describe the present invention in detail, production examples, formulation examples, and experimental examples of Yugao extract used in the present invention will be given as examples, but the present invention is not limited thereto. All the contents in the examples are weight%.
The production examples of Yugao extract and pomegranate extract are shown below.

Production Example 1
500 g of Yugao fruit was shredded, extracted by heating twice with 500 ml of water twice for 2 hours, and further concentrated by vacuum lyophilization to obtain 1 g of an extract (containing 99% or more solids).

Production Example 2
After thoroughly washing 100 g of camphor (commercially available product), it was heated and extracted twice with 1000 ml of water for 2 hours. After filtration of the residue, the filtrate was concentrated under reduced pressure, and further freeze-dried in vacuo to obtain 6 g of an extract (containing 99% or more solid matter).

Production Example 3
60 g of dried yuga fruit was pulverized, extracted by heating with 600 ml of a water-ethanol mixture (1: 1) for 5 hours, and further concentrated to obtain 2 g of an extract (containing 50% solids).

Production Example 4
60 g of dried yugao fruit was pulverized, 300 ml of ethanol was added, and the mixture was left at room temperature for 1 month. Further concentration gave 1 g (containing 99% or more solids).

Production Example 5
60 g of dried yugao fruit was pulverized, extracted twice with 600 ml of propanol at room temperature for 2 hours, and further concentrated to obtain 1 g of extract (including 70% solids).

Production Example 6 Pomegranate juice Commercially available concentrated pomegranate juice (Brix65) prepared by the pressing method was used.

Production Example 7 Fermentation liquid of pomegranate juice A commercially available concentrated pomegranate fruit juice (Brix65) was diluted with water to adjust the concentration to Brix15, and adjusted to pH 5 using an aqueous sodium hydroxide solution. An inoculum of lactic acid bacteria (derived from pickles) belonging to Lactobacillus plantarum was added to 500 g of the adjustment liquid. Fermented at 30 ° C. for 2 days and filtered to obtain 450 g of a pomegranate juice fermented product.

Production Example 8 Fermentation liquid of pomegranate juice A commercially available concentrated pomegranate juice (Brix65) was diluted with water to adjust the concentration to Brix11, and adjusted to pH 4.5 using an aqueous sodium hydroxide solution. An inoculum of lactic acid bacteria (derived from pickles) belonging to Lactobacillus plantarum was added to 500 g of the adjustment liquid. Fermented at 30 ° C. for 2 days and filtered to obtain 450 g of a pomegranate juice fermented product.

Production Example 9 Fermented liquid of pomegranate juice A commercially available concentrated pomegranate fruit juice (Brix65) was diluted with water to adjust the concentration to Brix8, and adjusted to pH 4.5 using an aqueous sodium hydroxide solution. An inoculum of lactic acid bacteria (derived from pickles) belonging to Lactobacillus plantarum was added to 500 g of the adjustment liquid. Fermented at 30 ° C. for 2 days and filtered to obtain 450 g of a pomegranate juice fermented product.

Production Example 10 Fermented liquid of pomegranate juice A commercially available concentrated pomegranate juice (Brix65) was diluted with water to adjust the concentration to Brix5, and adjusted to pH 4.5 using an aqueous sodium hydroxide solution. An inoculum of lactic acid bacteria (derived from pickles) belonging to Lactobacillus plantarum was added to 500 g of the adjustment liquid. Fermented at 30 ° C. for 2 days and filtered to obtain 450 g of a pomegranate juice fermented product.

Production Example 11 Pomegranate Juice Fermentation Liquid 210 g of pomegranate fruit fermentation product of Production Example 8 was added with 90 g of 1,3-butylene glycol and filtered to obtain 280 g of pomegranate juice fermentation product.

  Below, the prescription example using a Yugao extract and a pomegranate extract is shown.

Formulation Example 1 Lotion (Ingredient) (wt%)
1. Yugao extract (Production Example 2) 0.5
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.

Comparative Example 1 Conventional lotion In Formulation Example 1, a conventional lotion was obtained by replacing Yugao extract (Production Example 2) with purified water.

Formulation Example 2 Emulsion A
(Ingredient) (wt%)
1. Yugao extract (Production Example 4) 0.5
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10 to 13 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Comparative Example 2 Conventional Emulsion In Formulation Example 2, a conventional emulsion was prepared by replacing Yugao extract (Production Example 4) with purified water.

Formulation Example 3 Cream (ingredient) (wt%)
1. Yugao extract (Production Example 1) 0.5
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13.1,3-Butylene glycol 8.5
14 [Manufacturing method] Components 2 to 9 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Comparative Example 3 Conventional Cream A conventional cream was prepared by replacing Yugao extract (Production Example 1) with purified water in Formulation Example 3.

Formulation Example 4 Gel (Component) (wt%)
1. Yugao extract (Production Example 5) 0.5
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Perfume appropriate amount 6.1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Production method] Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved in purified water, and the two are mixed to obtain a product.

Formulation Example 5 Bath preparation (component) (wt%)
1. Yugao extract (Production Example 2) 1.0
2. Sodium bicarbonate 50.0
3. Yellow No. 202 (1) Appropriate amount 4. Perfume appropriate amount 5. [Manufacturing method] Ingredients 1 to 5 are mixed uniformly with sodium sulfate to make a product.

Formulation Example 6 Tablet (Ingredient) (wt%)
1. Yugao Extract (Production Example 1) 5.0
2. Dried corn starch 25.0
3. Carboxymethylcellulose calcium 20.0
4). Microcrystalline cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6). Talc 3.0
[Production method] Components 1 to 4 are mixed, and then an aqueous solution of component 5 is added as a binder to form granules. Ingredient 6 is added to the molded granules and compressed. One tablet is 0.52 g.

Formulation Example 7 Tablet Confectionery (Ingredient) (wt%)
1. Yugao Extract (Production Example 2) 2.0
2. Dried corn starch 49.8
3. Erythritol 40.0
4). Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6). Fragrance 0.1
7). Purified water 0.1
[Production method] Components 1 to 4 and 7 are mixed and granulated. Ingredients 5 and 6 are added to the molded granules and tableted. One tablet is 1.0 g.

Formulation Example 8 Emulsion B
(Ingredient) (wt%)
1. Yugao extract (Production Example 4) 0.5
2. Pomegranate fermentation broth (Production Example 11) 0.5
3. Squalane 5.0
4). Olive oil 5.0
5. Jojoba oil 5.0
6). Cetanol 1.5
7). Glycerol monostearate 2.0
8). Polyoxyethylene cetyl ether (20E.O.) 3.0
9. Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
10. Fragrance 0.1
11. Propylene glycol 1.0
12 Glycerin 2.0
13. Methyl paraoxybenzoate 0.2
14 [Production Method] Components 3 to 9 are heated and dissolved and mixed with purified water to 100, and the mixture is kept at 70 ° C. to obtain an oil phase. Ingredients 1-2 and 11-14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Formulation Example 9 Emulsion C
(Ingredient) (wt%)
1. Fermented liquor of pomegranate juice (Production Example 11) 0.5
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10 to 13 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

  In order to effectively explain the present invention, experimental examples are given below. In addition, this invention is not limited by this.

Experimental Example 1 Cathepsin V activity measurement 1
The effect of cathepsin V to promote enzyme activity was measured under the following conditions.

  The enzymatic reaction of cathepsin V consists of 50 ng human recombinant cathepsin V protein (R & D), 9 μM (final concentration) Z-Leu-Arg-7-Amino, 4-Methyl Coumarin (R & D) as a substrate, and 10 μg / ml (final) (Concentration) in a 110 μl reaction buffer (25 mM sodium acetate, 0.1 M NaCl, 5 mM dithiothreitol, pH 5.5) containing a sample of the extract The amount of the reaction product (7-Amino, 4-Methyl Coumarin) during the enzyme reaction was measured with a fluorescence plate reader SpectraMax Gemini EM (Molecular Device).

  The test results are shown in Table 1. The cathepsin V enzyme activity in the non-added condition was 57.6 pmol / min · μg enzyme. On the other hand, the cathepsin V enzyme activity under the condition of adding Yugao extract is 80.6 pmol / min · μg enzyme, which is 140% as compared with the non-adding condition, indicating that Yugao extract has the action of promoting the enzyme activity of Cathepsin V. It was.

The effect of cabbage V activity of Yugao extract

Experimental Example 2 Cathepsin V activity measurement 2
The effect of cathepsin V to promote enzyme activity was measured under the following conditions.

  The enzymatic reaction of cathepsin V was performed by using an ultrasonic crushed product of a horn plug collected from a cheek portion of a subject using a pore removal sheet as an enzyme source. 110 μl of reaction buffer containing 140 μg of corneal plug sonication, 9 μM (final concentration) Z-Leu-Arg-7-Amino, 4-Methyl Coumarin (R & D) as a substrate, and Yugao extract or cucumber extract ( 25 mM Sodium Acetate, 0.1 M NaCl, 5 mM dithiothreitol, pH 5.5) was performed at 37 ° C. The amount of the reaction product (7-Amino, 4-Methyl Coumarin) during the enzyme reaction was measured with a fluorescence plate reader SpectraMax Gemini EM (Molecular Device). For the cucumber extract, Cucumber Organic manufactured by Ichimaru Falcos Inc. containing 0.2% of cucumber extract was diluted to a desired concentration.

  The test results are shown in Table 2. The cathepsin V enzyme activity in the non-added condition was 0.105 pmol / min · μg protein. On the other hand, the cathepsin V enzyme activity under the condition of adding the red ginger extract is 0.117 pmol / min · μg protein when adding 1.0 μg / ml of the red ginger extract, and 0.138 pmol / min · μg protein when adding 10.0 μg / ml, 100 When added at 0.0 μg / ml, it is 0.142 pmol / min · μg protein, which is 112, 132, and 135%, respectively, compared to the non-added condition. It was shown to have an effect of Moreover, the cathepsin V enzyme activity in the cucumber extract addition condition is 0.096 pmol / min · μg protein, which is 91% compared to the non-addition condition and 68% compared to the addition condition of the Yugao extract. The specificity of the activity promoting action was confirmed.

Effects of Yugao extract and Cucumber extract on cathepsin V activity in horn plugs

Experimental example 3 Continuous test 1
Using Prescription Example 2 and Comparative Example 2 of Latex A containing Yugao extract, a one-month continuous test was conducted on males in their 20s to 50s and 8 female subjects. The test is a blind test in which the presence or absence of the active ingredient is not notified to the subject, and the prescription example 2 containing the active ingredient is continuously used in one half-face cheek of the subject, and the comparative example 2 is used continuously in the other half-face cheek. Twice a day in the morning and evening for 1 month. Porphyrin fluorescence, which is an index of horn plug formation, was measured for cheeks before and after one month of continuous use. At the same time, a replica of the cheek was collected with a replica agent SILFLO (FLEXICO), and the pore area ratio was calculated using the replica.

  The amount of porphyrin, which is an index of horn plugs, is obtained as a white pixel by obtaining an ultraviolet image of the cheek using a horn plug scope Charm View (Mortex), smoothing the image with a Gaussian filter, and binarizing it. It was obtained by quantifying the number of pixels of the fluorescent site to be detected. On the side where the prescription example 2 containing the active ingredient yugao extract was used continuously, the porphyrin fluorescence (number of pixels) was reduced compared to the side where the comparative example 2 was used continuously, and a difference between groups was observed (p < 0.05), the action of degrading horn plugs by Yugao extract was shown (Table 3).

Change in cheek porphyrin fluorescence (number of pixels)

  The replica collected from the cheek was photographed under a stereomicroscope with the light source incident angle set to 30 degrees. The obtained image was subjected to noise reduction processing due to bubbles and the like, and the area ratio was measured for the pores obtained by binarization, and the amount of change from before use was calculated. As a result, the pore area ratio decreased on the side where Formulation Example 2 containing Yugao extract, which is an active ingredient, was continuously used, compared with the side where Comparative Example 2 was used continuously, and a difference between groups was recognized (p < 0.05), the effect of improving the conspicuous pores by Yugao extract was shown (Table 4).

Change in pore area ratio of cheek

Experiment 4 Continuous test 2
Formulation Example 2 (milky lotion A) containing yugao extract, Formulation Example 8 (milky lotion B) in which 0.5% pomegranate juice fermentation liquid was added to Formulation Example 2, and Formulation example 9 containing a fermentation liquid of pomegranate juice Using (Emulsion C), a one-month continuous test was conducted on 18 female subjects in their 20s to 40s. The subject was informed of the presence or absence of the active ingredient in a blind test, and either prescription was applied continuously to the entire face of the subject, and the test was continued twice a day in the morning and evening for one month. Evaluation was performed using a pore score indicating the conspicuousness of pores in the cheeks, which was taken and measured using a full-face imaging diagnostic apparatus VISIA (Canfield Scientific) before and after one month of continuous use.

  The pore score calculated by VISIA contained Prescription Example 2 containing both the Yugao extract and the pomegranate juice fermentation solution, and the Prescription Example 2 containing the Yugao extract and the fermentation solution of the pomegranate juice. Since a significant decrease in the pore score was observed as compared with the case where Formulation Example 9 was used continuously (p <0.05), Yugao extract alone, or Yugao extract and pomegranate fermentation broth rather than just the fermentation broth of pomegranate juice. The effect of remarkably improving the conspicuousness of pores was shown when used (Table 5).

Change in cheek pore score

  The present invention has been clarified based on the localization of corneal plug-constituting protein, and promotes the activity of cathepsin V localized in the horn plug, thereby promoting the decomposition of horn plug, cathepsin V, An activity promoter and an agent for improving pore conspicuousness can be provided.

Claims (4)

  Cathepsin V activity promoter characterized by containing a Yugao extract.   An agent for promoting the degradation of horn plugs, which contains Yugao extract.   An agent for improving the conspicuous pores, characterized by containing a Yugao extract. An agent for improving pore conspicuousness, comprising a Yugao extract and a pomegranate extract.