JP2000121552A - Spr sensor cell and immunoreaction measuring device using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a SPR(surface plasmon resonance) sensor cell capable of measuring the immunoreaction of a plurality of specimens with a simple structure and an immunoreaction measuring device using the same. SOLUTION: A specimen cell 5 provided with a recessed part for storing a predetermined specimen, a lid member 7 to seal the opening part of the specimen cell 5, and an optical fiber 9 for sensors to be mounted to the lid member 7 are provided for constituting an SPR sensor cell. The face of one end part the optical fiber 9 for sensors is exposed to the outside from the lid member 7, an SPR sensor part 9d is formed at the region of the other end of the optical fiber 9 for sensors, and the SPR sensor part 9d is immersed in the specimen.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an immunological reaction measuring device, and more particularly to a so-called surface plasmon resonance (Surface) device.
The present invention relates to an SPR sensor cell utilizing the phenomenon of Plasmon Resonance (hereinafter abbreviated as "SPR") and an immunoreaction measuring device using the same.

[0002]

2. Description of the Related Art Conventionally, in the field of biochemical analysis, immunoassay has been widely used as a method for detecting an extremely small amount of protein in a specimen. In this immunization method, a predetermined antigen concentration in a specimen is quantified by a specific immune reaction between an antigen (a protein to be detected) and an antibody (an antibody produced using the antigen). This immunization method can measure even a specimen in which a plurality of types of antigens are mixed without isolating the antigen to be detected. This is different from the chemical measurement method or the physical measurement method.

[0003] Among the immunization methods, there are various methods as described below. radio immunoassay: RIA (radioimmunoassay) enzyme immunoassay: EIA (enzyme immunoassay) fluoroimmunassay: FIA (fluorescence immunoassay)

[0004] Since the RIA method requires the use of an isotope, it has not been widely used recently. Also, EI
The method A is widely used at present because the immune reaction can be easily measured. Furthermore, the FIA method is positioned as a highly sensitive and highly accurate measurement method. Among the EIA methods, a method using a solid phase for antibody measurement, particularly ELISA (enzymelinke)
d immunosorbent assay), and the ELISA has the following two methods.

A. Indirect method: a method using an antigen as a solid phase b. Antibody capture method: a method using an anti-IgM antibody as a solid phase

[0006] The above-mentioned ELISA method is used for quantification of an antibody against a specific pathogen, quantification of an antibody against an allergen, and screening of a monoclonal antibody. The measurement kit used for the ELISA method includes:
Generally, a microplate in which 96 concave portions are formed is used, and an immunoreaction measurement is performed on this microplate. Therefore, a large number of samples can be measured at the same time, and in recent years, many automated immune reaction measuring devices have been on the market.

As reagent kits for ELISA, various reagent manufacturers provide various reagents. For example, there is tPA, an enzyme that acts indirectly in the direction of dissolving fibrin involved in blood coagulation and thrombus in blood. PAI-1 is an enzyme that suppresses tPA and acts in the direction of blood coagulation and thrombus formation.

By the way, a so-called SPR sensor is known as a sensor used in an immune reaction measuring device.
This SPR sensor is a sensor using the surface plasmon resonance phenomenon, and an immune reaction is measured based on the following principle.
That is, a metal thin film (such as gold or silver) having a thickness of about 50 nm is deposited on the bottom surface of the prism having a high refractive index. Then, predetermined light is incident from the prism side toward the metal thin film at an angle equal to or greater than the critical angle. Since the metal thin film is translucent at about 50 nm, the light incident from the prism side passes through the metal thin film, reaches the surface of the metal thin film on the side opposite to the prism, and reaches the surface of the metal thin film on the side opposite to the prism. Generate an evanescent field.

By adjusting the incident angle of light, the wave number of the evanescent field and the wave number of surface plasmon resonance can be matched, and surface plasmon resonance can be excited on the surface of the metal thin film. In this case, the wave number of surface plasmon resonance depends on the dielectric constant of the metal thin film and the refractive index of the substance fixed on the surface opposite to the prism when viewed from the metal thin film. Therefore, the refractive index and the dielectric constant with respect to the fixed substance can be checked. As described above, since the optical system and the sample are located on opposite sides of the metal thin film as a boundary, it is easy to construct a sensor.

By applying the above principle, an SPR sensor for an immune reaction measuring device using an optical fiber has been developed (B
Manufactured by IACORE-trade name: BIACORE Probe). In the SPR sensor using this optical fiber, first, the clad on the outer peripheral surface of the distal end portion of the optical fiber is removed, and the end surface of the distal end of the optical fiber is cut or polished. Coated. Further, the outer peripheral surface of the tip region of the optical fiber is coated with a metal thin film (such as gold or silver). Further, the metal thin film on the outer peripheral surface of the distal end of the optical fiber is covered with a dielectric film, and the antibody used for the immunological reaction measurement is fixed on the dielectric film. Further, light from a predetermined light source can be introduced into the other end of the optical fiber.

A method for measuring the immune response of the SPR sensor having the above-described configuration will be described. First, as for light introduced into the optical fiber, light of a specific wavelength excites surface plasmon resonance at the tip of the optical fiber. The wavelength of the light that excites the surface plasmon resonance changes depending on the refractive indices of the dielectric film and the antibody. The intensity of light having a wavelength that causes surface plasmon resonance is attenuated. Therefore, the immune response can be measured by comparing the wavelength of the light that attenuates the most before the immune reaction with the wavelength of the light that attenuates the most after the immune reaction.

[0012]

However, each of the above-mentioned prior arts has the following disadvantages. That is, when the SPR sensor is configured by an optical fiber, the SPR sensor and the immune reaction measuring device are conventionally configured by using one optical fiber. For this reason, when measuring the immune reaction of many kinds of specimens, or when performing the measurement of the immune reaction of multiple items for one specimen, the sensing unit of the optical fiber must be replaced each time. Therefore, there has been an inconvenience that it takes a long time to perform a series of immune reaction measurements.

Also, the SPR sensor cell and the immunoreactivity measuring device using the prism have the following disadvantages. That is, a prism type SPR sensor that emits light of a short wavelength is used as a light source. The immune reaction measurement is performed using the relationship between the incident angle of light and the light intensity. For this reason, there has been an inconvenience that a special drive unit for appropriately adjusting the incident angle of light to the prism is required.

[0014]

An object of the present invention is to improve the disadvantages of the prior art and, in particular, to provide an SPR sensor cell capable of rapidly measuring the immunoreactivity of a plurality of specimens with a simple structure, and an immunoreactivity measurement device using the same. The purpose is to provide.

[0015]

In order to achieve the above object, according to the first aspect of the present invention, an SPR sensor cell includes a sample cell having a concave portion for storing a predetermined sample, and an SPR sensor cell. A lid member for sealing the opening of the cell,
A sensor optical fiber attached to the lid member,
The end face of one end of the optical fiber for sensor is exposed to the outside from the lid member, the SPR sensor section is formed in the other end area of the optical fiber for sensor, and the SPR sensor section is immersed in the sample. I have.

With the above-described configuration, when measuring an immune reaction, a sample to be measured is first injected into a sample cell. The sample is injected using a micropipette through a through hole formed in the lid member. As a result, the sample comes into contact with the SPR sensor section formed on the sensor optical fiber. An antibody (or antigen) is fixed to the SPR sensor via a dielectric film. Therefore, if an antigen (or antibody) specifically reacting with the aforementioned antibody (or antigen) is contained in the specimen, an immune reaction occurs.

When light is introduced into the sensor optical fiber when an immune reaction occurs, the light intensity of light of a specific wavelength is attenuated in response to the immune reaction. Therefore, by introducing the light reflected through the optical fiber for the sensor into the spectroscope and analyzing the wavelength distribution of the light, it is possible to measure an immune reaction.

[0018]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described with reference to FIG. Here, FIG. 1A is a sectional perspective view showing the SPR sensor cell 3 of the present embodiment, and FIG.
(B) is an exploded perspective view of the SPR sensor cell 3.
The SPR sensor cell 3 includes a cup-shaped sample cell 5 and
A lid member 7 for sealing the opening of the sample cell 5 and a sensor optical fiber 9 mounted on the lid member 7 are provided. Hereinafter, these will be described in detail.

[Specimen Cell] The specimen cell 5 has a cup shape formed by combining a hollow cylinder and a hollow cone. The material of the sample cell 5 is plastic. However, since the sample cell 5 is for storing a sample as described later,
The shape and material are not particularly limited. For example, the shape may be a simple cylindrical shape or a conical shape, and the material may be metal or glass.

Further, around the opening side of the sample cell 5,
A predetermined flange 5a is formed. This collar 5a
Is used when the SPR sensor cell 3 is mounted on a cell fixing base (not shown) as described later. In the present embodiment, the flange 5a is formed integrally with the sample cell 5. However, the flange portion 5a may be configured separately, and the flange portion 5a may be joined to the sample cell 5 ex post facto. In addition, if the cell fixing base has a special structure and the sample cell 5 can be securely fixed, the flange portion 5a can be omitted.

[Cover Member] The cover member 7 seals the opening of the sample cell 5 and has a circular shape substantially equal to the inner diameter of the opening of the sample cell 5. The specific shape includes a fitting portion 7a that fits inside the sample cell 5 and a disk-shaped locking portion 7b that comes into contact with the flange 5a of the sample cell 5 described above. However, in the present embodiment, the fitting portion 7a and the locking portion 7b are integrally formed of plastic.

An optical fiber fixing hole 7c for fixing the sensor optical fiber 9 is formed at a substantially central portion of the cover member 7. The optical fiber fixing hole 7 c extends in the thickness direction of the cover member 7. When the optical fiber for sensor 9 is fixed, the tip of the optical fiber for sensor 9 reaches near the bottom of the sample cell 5. I do. This sensor optical fiber 9 is SP
It is used as an R sensor and will be described later in detail.

The cover member 7 has an optical fiber fixing hole 7c.
Are formed, two through holes 7d are formed. These through holes 7d are used when a sample is injected into the sample cell 5 or when the sample is aspirated. The two through holes 7d are formed when the sample is injected from one through hole and the sample cell 5 is inserted from the other through hole.
This is to remove the air inside.

As described above, in this embodiment, the lid member 7
Is made of plastic, but may be made of other materials (metal, glass, ceramics, etc.). Further, the surface of the lid member 7 may be covered with a light absorbing agent (for example, black ink or the like). This is to prevent unnecessary light from entering the sample cell 5 and improve the accuracy of the immune reaction measurement. The cover member 7 has one optical fiber fixing hole 7.
Although c is formed, the present invention is not limited to this. That is, a plurality of sensor optical fibers may be fixed. By immobilizing different antibodies (or antigens) on the surfaces of a plurality of optical fibers for sensors, and bringing the samples into contact with the optical fibers for sensors, it is possible to measure a variety of immunoreactions for one type of sample. Can be performed quickly.

The through hole 7d formed in the cover member 7 also
It is not always necessary to have two. That is, as shown in FIG. 2, even if the through-hole 7g is formed in the lid member 7e, it is only necessary to discharge the air in the sample cell 5 while injecting the sample. Further, the shape of the through-hole does not need to be cylindrical, and may be formed in a tapered shape.

[Optical Fiber for Sensor] As shown in FIGS. 1 and 3, the SPR sensor of this embodiment is constituted by an optical fiber 9 for sensor. FIG. 3A schematically shows the optical fiber 9 for a sensor, and the difference in the thickness of each part is emphasized for clarity of explanation. The sensor optical fiber 9 used in the present embodiment is a general one. That is, at the center of the sensor optical fiber 9, there is a core 9a for transmitting light. The outer peripheral surface of the core 9a is covered with a clad 9b having a different refractive index from the core. Further, a primary coating 9c as a protective film is formed on the outer peripheral surface of the clad 9b.

The material of the core 9a is a general one. Specifically, it is glass or transparent plastic. The cladding 9b is also made of glass or plastic. Further, the primary coating 9c is made of plastic.
The material of the core 9a is not particularly limited as long as it can transmit light. That is, any material having a predetermined transparency can be applied to the present invention.

The distal end region of the sensor optical fiber 9 (FIG. 3)
The lower region (A) is the SPR sensor section 9d. More specifically, after the primary coating 9c and the cladding 9b are removed, the outer peripheral surface of the core 9a is coated with a metal thin film 9e. Various materials can be considered as the material of the metal thin film 9e. In the present embodiment, Au is used as an example. However, the material is not limited as long as it causes the surface plasmon resonance phenomenon. For example, Ag, Pt, etc. can be used. Vapor deposition or the like is used to form the metal thin film 9e. A metal thin film 9f for reflection is also formed on the tip of the optical fiber 9 for sensor. This functions as a mirror for reflecting the light traveling inside the core 9a. As a material of the metal thin film 9f to be a mirror, for example, Ag or Al is used. However, the material of the metal thin film 9f serving as a mirror is not particularly limited as long as it can reflect light.

Further, a dielectric film (not shown) is fixed on the surface of the metal thin film 9e formed on the outer peripheral surface of the core 9a, and an antibody (or antigen) is fixed on the surface of the dielectric film. . However, in FIG. 3, the dielectric film and the antibody (or antigen) are omitted. The antibody (or antigen) formed on the surface of the dielectric film is appropriately selected according to the antigen (or antibody) to be measured.

The sensor optical fiber 9 is inserted into an optical fiber fixing hole 7c formed in the cover member 7, and is fixed to the cover member 7 with an adhesive. As a means for fixing the sensor optical fiber 9, in addition to the adhesive, the sensor optical fiber 9 may be directly fused to the cover member 7, or the sensor optical fiber 9 may be simply inserted. When the sensor optical fiber 9 is simply inserted, the lid member 7 and the sample cell 5 can be used again.

[Immune Reaction Measuring Apparatus] Next, the above SP
The immune reaction measuring device 1 using the R sensor cell 3 will be described. The main components of the immune response measurement device 1 according to the present embodiment include a light source 11 that emits light for measuring an immune response, the above-described SPR sensor cell 3, and the SPR sensor cell 3.
A spectroscope 13 that receives light reflected from the sensor cell 3 and analyzes the wavelength distribution.

[Light Source] The light source 11 emits light of a wide wavelength band, and specifically, a halogen lamp, a xenon lamp, or the like is used. In the immune response measuring device 1 of the present invention, the immune response is measured by analyzing the change in the wavelength distribution of the light before being incident on the SPR sensor cell 3 and the reflected light. Therefore, it is desirable that the light source 11 emits light having a stable wavelength distribution. Note that a light source other than the above-described halogen lamp or xenon lamp may be used as long as it can emit light having a wavelength distribution in a high band.

Downstream of the light source 11, the condenser lens 1
5, a receptacle 17, an optical connector 19, and a light source optical fiber 21 are provided. The light source optical fiber 21 is connected to an optical coupler 23 described later. Accordingly, the light emitted from the light source 11 is condensed by the condenser lens 15 and is incident on the receptacle 17, and is incident on the optical coupler 23 via the optical connector 19 and the optical fiber 21 for the light source.

[Spectroscope] The spectroscope 13 analyzes the wavelength distribution of the light reflected from the SPR sensor cell 3. When the surface plasmon resonance phenomenon occurs in the SPR sensor cell 3, the intensity of light having a specific wavelength is attenuated.
This decaying wavelength varies with the immune response. Therefore, by measuring the change in the wavelength distribution of the reflected light by the spectroscope 13, an immune reaction measurement can be performed.

The upstream side of the spectroscope 13 (the optical coupler 2 described later)
3), a condenser lens 25, a receptacle 27, an optical connector 29, and an optical fiber 31 for a spectroscope are sequentially provided. That is, the light reflected from the SPR sensor cell 3 and returned to the optical coupler 23 is transmitted to the spectroscope optical fiber 31.
Through the optical connector 29. Then, the light exits from the receptacle 27 and enters the condenser lens 25.
In the condenser lens 25, the light is appropriately condensed and guided to the spectroscope 13.

[Optical Coupler] An optical coupler 23 is provided between the light source 11 and the spectroscope 13. The optical coupler 23 has a role of transmitting light from the light source 11 to the SPR sensor cell 3 and transmitting light reflected from the SPR sensor cell 3 to the spectroscope 13.

The optical coupler 23 includes a relay optical fiber 33 and an optical connector 3 in order toward the SPR sensor cell 3.
5, a receptacle 37 and a condenser lens 39 are provided. By providing such an optical system, light for measuring an immune reaction is transmitted to the SPR sensor cell 3.

[Operation] The operation of the immune reaction measuring apparatus 1 according to the present invention will be described with reference to FIG. First, the light emitted from the light source 11 is condensed by the condenser lens 15 and is introduced into the receptacle 17. Receptacle 17
Incident on the optical fiber for light source 21 via the optical connector 19. Furthermore, the light source optical fiber 21
Is incident on the optical coupler 23.

The light incident on the optical coupler 23 is incident on the relay optical fiber 33 on the SPR sensor cell 3 side, and further is incident on the condenser lens 39 through the optical connector 35 and the receptacle 37. The light transmitted through the condenser lens 39 is
Sensor optical fiber 9 provided in SPR sensor cell 3
(See FIG. 1). Here, the end surface of the sensor optical fiber 9 is positioned near the focal point of the condenser lens 39 so that light is appropriately introduced into the sensor optical fiber 9.

The light incident on the sensor optical fiber 9 is S
It reaches the PR sensor section 9d. Here, when surface plasmon resonance occurs in the SPR sensor unit 9d, the light intensity of light of a specific wavelength is attenuated. Then, the light is reflected on the end face of the sensor optical fiber 9 and returns to the path. That is, the light that has exited the sensor optical fiber 9 is incident on the optical coupler 23 through the condenser lens 39, the receptacle 37, the optical connector 35, and the relay optical fiber 33. In the optical coupler 23, the light is branched, and a part of the light is introduced into the optical fiber 31 for a spectroscope.

The light incident on the optical fiber for spectroscope 31 is transmitted to the optical connector 29, the receptacle 27, and the condenser lens 2.
5 and enters the spectroscope 13. In the spectroscope 13,
The wavelength distribution of the incident light is analyzed. Here, the immune response can be measured by comparing the wavelength distribution when no immune response has occurred and the wavelength distribution after the immune response has occurred.

[Other Embodiments] FIG. 5 shows another example of the immune reaction measuring apparatus of the present invention. Here, the same components as those in the above-described embodiment will be described with the same reference numerals. This immunoreaction measurement device 51 is different from the above-described immunoreaction measurement device in that it further includes a condenser lens 14a and a plurality of optical fibers 14b between the light source 11 and the condenser lens 15. Other basic components are common. As described above, by providing the plurality of optical fibers 14a, light with high light intensity can be supplied to the SPR sensor cell 3.

FIG. 6 shows another example of the immunological reaction measuring device. The immunoreaction measuring device 61 differs from the above-described immunoreaction measuring devices in that a fused optical fiber array 14c and an optical connector 14d are disposed between the condenser lens 15 and the receptacle 17. And the other basic components are common. FIG. 7 is a sectional view showing a specific configuration of the fused optical fiber array 14c. This fusion type optical fiber array 14c is composed of three optical fibers.
The books are bundled, and the junction is joined to each other by heating and melting. Then, the three optical fibers are connected to the light source 11.
An optical connector 14d is fixed to the other one of the optical fibers so as to correspond to the condenser lens 15 on the side. As described above, by providing three optical fibers on the light source 11 side, it is possible to supply light having higher intensity than that of one optical fiber to the SPR sensor cell.

FIG. 8 shows a plurality of SPR sensor cells 3a, 3
Regarding b ..., an immune reaction measuring device 71 in the case of sequentially performing an immune reaction measurement is shown. The basic components of the immunoreaction measurement device are the same as those described above, but the sensor cell fixing table 4
1 in that a plurality of SPR sensor cells 3 are mounted on the same. And when measuring the actual immune response,
The sensor cell fixing base 41 is movable.

FIG. 8 shows the first SPR sensor cell 3a.
Indicates a state in which is positioned at a position facing the condenser lens 39. In this figure, the sensor cell fixing base 4
1 is described as a cross section. After the immunoreactivity measurement has been performed on the first SPR sensor cell 3a, the sensor cell fixing base 41 moves and the second SPR sensor cell 3b is positioned at a position facing the condenser lens 39. Then, an immune reaction measurement is performed. 8, six SPR sensor cells 3a, 3b...
It is placed on. Therefore, after the completion of the measurement of the six types of immune reactions, the measurement of the immune reactions is performed using another SPR sensor cell. Note that the number of SPR sensor cells is an example, and is not particularly limited to six.

FIGS. 9 and 10 show the sensor cell fixing table 43.
FIG. FIG. 9 shows a sensor cell fixing base 43 on which one SPR sensor cell 3 can be placed. The sensor cell fixing base 43 has a plate-like upper surface, and has a sensor cell fixing hole 43 for inserting an SPR sensor cell in the center thereof.
a is formed. FIG. 10 shows a sensor cell fixing base 45 on which eight SPR sensor cells 3a to 3h can be placed at the same time.
Is shown. As described above, the plurality of SPR sensor cells 3a to 3
By mounting h, other types of immune reaction measurement can be performed quickly.

FIGS. 11 and 12 also show a sensor cell fixing base on which a plurality of SPR sensor cells can be mounted. FIG.
The PR sensor cells are arranged in two rows, and FIG. 12 is arranged on a circumference. When the sensor cell fixing table 47 shown in FIG. 11 is moved, it is necessary to perform the parallel movement twice. On the other hand, in the sensor cell fixing table 49 shown in FIG. 12, by rotating the sensor cell fixing table itself, an immune reaction measurement can be performed for each SPR sensor cell. In addition, a solenoid, a stepping motor, a micromotor, or the like is used for moving the sensor cell fixing base.

[0048]

As described above, in the SPR sensor cell of the present invention, a sample cell having a concave portion for storing a predetermined sample, a lid member for sealing an opening of the sample cell, A sensor optical fiber to be mounted, exposing one end of the sensor optical fiber to the outside from the cover member, and connecting the sensor optical fiber to the other end region of the sensor optical fiber.
A PR sensor part was formed, and the SPR sensor part was immersed in the sample. By configuring the SPR sensor cell in this manner, the SPR sensor cell can be configured at low cost and small size. Further, since the SPR sensor cell itself is independent from other components of the immunoreaction measurement device, replacement of the SPR sensor cell can be easily performed.

[0049] In addition, since the replacement of the SPR sensor cell is simple, there is an excellent effect that the immunological reaction measurement for a plurality of types of specimens can be performed quickly. Furthermore, since only the SPR sensor cell can be discarded, there is an excellent effect that the usability and hygiene reliability can be improved.

[Brief description of the drawings]

1 is a perspective view showing an SPR sensor cell according to an embodiment of the present invention, FIG. 1 (A) is a sectional perspective view after assembly, and FIGS. 1 (B) and 1 (C) are exploded perspective views. The figure is shown.

2 is a diagram showing another example of a lid member used in the SPR sensor cell disclosed in FIG. 1, FIG. 2 (A) is an overall perspective view, and FIG. 2 (B) is a side view.

3A and 3B show an optical fiber for a sensor used in the SPR sensor cell disclosed in FIG. 1, FIG. 3A is an overall perspective view, and FIGS. 3B to 3D are FIGS. 3) shows a cross-sectional view of each part.

FIG. 4 is a block diagram showing an immune reaction measurement device using the SPR sensor cell disclosed in FIG. 1;

FIG. 5 is a block diagram showing another example of an immunoreaction measurement device using the SPR sensor cell disclosed in FIG. 1;

FIG. 6 is a block diagram showing another example of the immunoreaction measurement device using the SPR sensor cell disclosed in FIG. 1;

7 is a cross-sectional view showing a fused optical fiber array used in the immunoreaction measurement device disclosed in FIG. 6, and FIG.
7A is a longitudinal sectional view of the optical fiber, and FIG.
(B) and FIG. 7 (C) show cross-sectional views perpendicular to the longitudinal direction.

FIG. 8 is a block diagram showing an immunoreaction measurement device of the present invention, particularly one equipped with a plurality of SPR sensor cells.

FIG. 9 is a perspective view showing a sensor cell fixing base used in the immunoreaction measurement device of the present invention. FIG. 9 (A) shows a state where an SPR sensor cell is not mounted, and FIG. 9 (B) shows an SPR sensor cell. This shows a state where the sensor cell is mounted.

FIG. 10 is a perspective view showing another example of a sensor cell fixing base used in the immunoreaction measurement device of the present invention.
10A shows a sensor cell fixing base on which a plurality of SPR sensor cells can be placed in a line, and FIG. 10B shows a state where a plurality of SPR sensor cells are placed.

FIG. 11 is a perspective view showing another example of a sensor cell fixing base used in the immune reaction measuring device of the present invention,
11A shows a sensor cell fixing base on which a plurality of SPR sensor cells can be placed in two rows, and FIG. 11B shows a state where a plurality of SPR sensor cells are placed.

FIG. 12 is a perspective view showing another example of a sensor cell fixing base used in the immune reaction measuring device of the present invention.
FIG. 12A shows a sensor cell fixing base on which a plurality of SPR sensor cells can be mounted on a circumference, and FIG.
This shows a state where the sensor cell is mounted.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Immune reaction measuring device 3 SPR sensor cell 5 Sample cell 7 Lid member 7c Optical fiber fixing hole 7d Through hole 9 Optical fiber for sensor 9d SPR sensor part 9e Metal thin film 9f Metal thin film for reflection 41 Sensor cell fixing stand

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 2G059 AA01 AA05 BB13 CC17 DD12 DD13 EE02 EE12 FF03 FF07 FF11 GG10 JJ01 JJ11 JJ13 JJ17 KK01 LL01 PP01

Claims (6)

[Claims] 1. A sensor cell comprising: a sample cell having a recess for storing a predetermined sample; a lid member for sealing an opening of the sample cell; and a sensor optical fiber mounted on the lid member. An end surface of one end of the optical fiber for use is exposed to the outside from the lid member, an SPR sensor portion is formed in the other end region of the optical fiber for sensor, and the SPR sensor portion is immersed in the sample. SPR sensor cell. 2. The SP according to claim 1, wherein a through hole for injecting the sample is formed in the lid member.
R sensor cell. 3. The SPR sensor cell according to claim 1, wherein the SPR sensor unit is made of a metal thin film of Au, Ag, or Pt coated on a core of the sensor optical fiber. 4. The SPR sensor cell according to claim 1, wherein a reflection metal thin film made of Ag or Al is formed as a reflection mirror on an end face of the other end of the sensor optical fiber. . 5. An S cell comprising a sample cell having a concave portion for storing a predetermined sample, a lid member for sealing an opening of the sample cell, and a sensor optical fiber mounted on the lid member.
A PR sensor cell; a light source that emits light in a predetermined wavelength band to the SPR sensor cell; and a spectroscope that receives light reflected from the SPR sensor cell and analyzes the wavelength distribution of the light. An end surface of one end of the optical fiber is exposed to the outside from the lid member, an SPR sensor portion is formed in the other end region of the optical fiber for the sensor, and the SPR sensor portion is immersed in the sample. Reaction measurement device. 6. An immunity comprising a plurality of said SPR sensor cells, each said SPR sensor cell being mounted on a predetermined sensor cell fixing base, and said sensor cell fixing base being movable along the arrangement direction of the SPR sensor cells. Reaction measurement device.