GB2539857A - Devices and methods for purification, detection and use of biological cells - Google Patents

Devices and methods for purification, detection and use of biological cells Download PDF

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Publication number
GB2539857A
GB2539857A GB1618356.8A GB201618356A GB2539857A GB 2539857 A GB2539857 A GB 2539857A GB 201618356 A GB201618356 A GB 201618356A GB 2539857 A GB2539857 A GB 2539857A
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GB
United Kingdom
Prior art keywords
column
cells
methods
devices
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1618356.8A
Other versions
GB201618356D0 (en
GB2539857B (en
Inventor
T Gjerde Douglas
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Individual
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Individual
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Filing date
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Application filed by Individual filed Critical Individual
Priority to GB2102155.5A priority Critical patent/GB2587768B/en
Publication of GB201618356D0 publication Critical patent/GB201618356D0/en
Publication of GB2539857A publication Critical patent/GB2539857A/en
Application granted granted Critical
Publication of GB2539857B publication Critical patent/GB2539857B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/428—Frontal mode
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.
GB1618356.8A 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells Active GB2539857B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2102155.5A GB2587768B (en) 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461974950P 2014-04-03 2014-04-03
US201462061636P 2014-10-08 2014-10-08
PCT/US2015/024374 WO2015154048A1 (en) 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells

Publications (3)

Publication Number Publication Date
GB201618356D0 GB201618356D0 (en) 2016-12-14
GB2539857A true GB2539857A (en) 2016-12-28
GB2539857B GB2539857B (en) 2021-03-31

Family

ID=54241352

Family Applications (2)

Application Number Title Priority Date Filing Date
GB2102155.5A Active GB2587768B (en) 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells
GB1618356.8A Active GB2539857B (en) 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB2102155.5A Active GB2587768B (en) 2014-04-03 2015-04-03 Devices and methods for purification, detection and use of biological cells

Country Status (3)

Country Link
EP (1) EP3126027A4 (en)
GB (2) GB2587768B (en)
WO (1) WO2015154048A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201521784D0 (en) * 2015-12-10 2016-01-27 Univ Birmingham Cell purification
JP7173671B2 (en) * 2018-10-09 2022-11-16 シー テクノロジーズ、インコーポレーテッド Mass control system for chromatography

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215927A (en) * 1986-01-30 1993-06-01 Fred Hutchinson Cancer Research Center Method for immunoselection of cells using avidin and biotin
US6362008B1 (en) * 1997-12-23 2002-03-26 Barbara A. Kohn Generic signalling mechanism for detection of analytes
US20040121382A1 (en) * 2002-12-18 2004-06-24 Linda Liu Multiplexed immunohistochemical assays using molecular tags
WO2013124474A2 (en) * 2012-02-23 2013-08-29 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
WO2014001326A1 (en) * 2012-06-27 2014-01-03 F. Hoffmann-La Roche Ag Method for the selection and production of tailor-made, selective and multi-specific therapeutic molecules comprising at least two different targeting entities and uses thereof
US20140234890A1 (en) * 2013-02-15 2014-08-21 Douglas T. Gjerde Methods for Purifying Biological Cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773224A (en) * 1996-02-12 1998-06-30 Grandics; Peter Immunoselection system for cell elution
US8377715B2 (en) * 2003-07-14 2013-02-19 Phynexus, Inc. Method and device for sample preparation
US20090081084A1 (en) * 2003-07-14 2009-03-26 Gjerde Douglas T Low Dead Volume Extraction Column Device
US20100140173A1 (en) * 2004-12-03 2010-06-10 Chris Suh Method and Device for Gravity Flow Chromatography
GB2525562B (en) * 2013-02-15 2017-10-11 T Gjerde Douglas Methods for purification of biological cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215927A (en) * 1986-01-30 1993-06-01 Fred Hutchinson Cancer Research Center Method for immunoselection of cells using avidin and biotin
US6362008B1 (en) * 1997-12-23 2002-03-26 Barbara A. Kohn Generic signalling mechanism for detection of analytes
US20040121382A1 (en) * 2002-12-18 2004-06-24 Linda Liu Multiplexed immunohistochemical assays using molecular tags
WO2013124474A2 (en) * 2012-02-23 2013-08-29 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
WO2014001326A1 (en) * 2012-06-27 2014-01-03 F. Hoffmann-La Roche Ag Method for the selection and production of tailor-made, selective and multi-specific therapeutic molecules comprising at least two different targeting entities and uses thereof
US20140234890A1 (en) * 2013-02-15 2014-08-21 Douglas T. Gjerde Methods for Purifying Biological Cells

Also Published As

Publication number Publication date
EP3126027A1 (en) 2017-02-08
GB202102155D0 (en) 2021-03-31
GB201618356D0 (en) 2016-12-14
GB2539857B (en) 2021-03-31
GB2587768B (en) 2021-08-18
WO2015154048A1 (en) 2015-10-08
EP3126027A4 (en) 2017-11-01
GB2587768A (en) 2021-04-07

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