CN217304824U - Sample analyzer and particle measuring device - Google Patents

Abstract

The embodiment of the utility model discloses sample analyzer and particle survey device for reduce optical system's among sample analyzer and the particle survey device volume. The embodiment of the utility model provides an in sample analyzer includes: sampling device, reagent device, sample preparation facilities and detection device, wherein, detection device includes: the front light assembly is used for providing an incident light beam for irradiating the blood sample to be detected; the flow chamber is used for providing a place irradiated by incident beams for the blood sample to be detected when the blood sample to be detected flows under the wrapping of the sheath liquid; the optical fiber coupler comprises an incident end, an optical fiber and an emergent end, wherein the incident end is used for coupling a scattering signal generated after an incident beam interacts with a blood sample to be detected in the flow chamber into the optical fiber and enabling the scattering signal to reach the emergent end through the optical fiber; and the photoelectric detector is used for detecting the scattering signal of the emergent end and outputting an electric signal.

Description

Sample analyzer and particle measuring device

The priority of the chinese patent application entitled "a particle analyzer and a sample analyzer" filed by the chinese patent office on 30/10/2021, application No. 202111278240.4, is claimed, and the entire contents of the application are incorporated herein by reference.

Technical Field

The utility model relates to a biochemical immunity technical field especially relates to a sample analyzer and particle survey device.

Background

Conventionally, a particle measuring apparatus using an optical flow cytometer is known. In such a particle measurement apparatus, a sample containing particles to be tested, such as blood, is caused to flow through a flow cell, and light emitted from a light source is irradiated to the flow channel. Then, the light emitted from each particle is detected by a signal detection device, and the particles are classified and counted based on the detected light.

Most of the current blood cell analyzers use the laser scattering principle for measurement, and the optical system with the fluorescence detection platform mainly comprises a forward scattering signal, a side 90-degree scattering signal and a side 90-degree fluorescence signal. In order to ensure that light energy at various angles is effectively collected, the current collection light path generally adopts free-space light transmission, and the relative positions of various optical elements on the collection light path are set, so that cell scattered light enters a signal detection device through space transmission, and a scattered signal is formed.

The disadvantage of this solution is that the requirement for the spatial position of each optical element is high when transmitting through free space light, which generally results in a large overall volume of the optical system, and the scattered signal to noise ratio is poor because the free space light is greatly affected by stray light.

Disclosure of Invention

The embodiment of the application provides a sample analyzer and a particle measuring device, which are used for reducing the volume of an optical system in the sample analyzer and the particle measuring device and improving the signal to noise ratio of a measured optical signal.

A first aspect of embodiments of the present application provides a sample analyzer, including:

a sampling device for collecting a blood sample;

the reagent device is used for bearing a reagent;

the sample preparation device is used for mixing the blood sample and the reagent in the reaction pool to prepare a blood sample to be detected;

the detection device is used for detecting specific items of the blood sample to be detected so as to obtain a detection result;

wherein the detection device comprises: the front light assembly is used for providing an incident light beam for irradiating the blood sample to be detected;

the flow chamber is used for providing a place where the blood sample to be detected is irradiated by the incident light beam when the blood sample to be detected flows through the sheath fluid in a wrapping mode;

the optical fiber coupler comprises an incident end, an optical fiber and an emergent end, wherein the incident end is used for coupling a scattering signal generated after the incident beam interacts with the blood sample to be detected in the flow chamber into the optical fiber and enabling the scattering signal to reach the emergent end through the optical fiber;

and the photoelectric detector is used for detecting the scattering signal of the emergent end and outputting an electric signal.

Preferably, the scatter signal comprises at least one of a forward scatter signal, a side scatter signal and a fluorescence signal.

Preferably, the forward scatter signal comprises at least one of a first angle forward scatter signal and a second angle forward scatter signal;

wherein the forward scatter signal of the first angle comprises: a scattered signal with an included angle of 1 to 10 degrees with the optical axis of the incident beam;

the forward scatter signal of the second angle comprises: and the included angle between the optical axis of the incident light beam and the scattering signal is in the range of 20-45 degrees.

Preferably, the side scatter signals include: and the included angle between the optical axis of the incident light beam and the scattered signal is in the range of 70 to 110 degrees.

Preferably, the scattered signal comprises the forward scattered signal, the sample analyzer further comprising:

the diaphragm is used for filtering emergent light beams which do not interact with the blood sample to be detected when the incident light beams penetrate through the flow chamber, and screening forward scattering signals generated after the incident light beams interact with the blood sample to be detected when the incident light beams penetrate through the flow chamber, so that the forward scattering signals are emitted into the optical fiber coupler.

Preferably, the diaphragm has a first semicircular opening and a second semicircular opening, and a light shielding member is disposed between the first semicircular opening and the second semicircular opening, wherein the forward scattering signal passes through the first semicircular opening and the second semicircular opening, and the emergent light beam that does not interact with the blood sample to be measured is blocked by the light shielding member.

Preferably, the diaphragm is disposed at a focal position of the emergent light beam that does not interact with the blood sample to be measured.

Preferably, the sample analyzer further comprises a first optical assembly disposed between the flow cell and the diaphragm, wherein the first optical assembly is configured to converge the forward scatter signal and the emergent light beam that has not interacted with the blood sample to be measured.

Preferably, the diaphragm is disposed at a focal position of the first optical assembly.

Preferably, the scattered signal further includes the side scattered signal, the fiber coupler includes a first fiber coupler and a second fiber coupler, and the photodetector includes a first photodetector and a second photodetector;

wherein the first fiber coupler is configured to couple the forward scatter signal such that the first photodetector detects the forward scatter signal;

the second optical fiber coupler is used for coupling the side scattering signal so that the second photoelectric detector detects the side scattering signal.

Preferably, the scattered signal further includes the fluorescence signal, the fiber coupler further includes a third fiber coupler, and the photodetector further includes a third photodetector;

wherein the third fiber coupler is configured to couple the fluorescence signal, so that the third photodetector detects the fluorescence signal.

Preferably, the sample analyzer further includes an optical filter disposed between the exit end of the third fiber coupler and the third photodetector, so as to filter stray light signals except the fluorescence signal.

Preferably, the sample analyzer further comprises a light splitting assembly, wherein,

the light splitting assembly and the flow chamber are arranged coaxially in the lateral direction, and the light splitting assembly is used for splitting the lateral scattering signal and the fluorescence signal, wherein the split lateral scattering signal is coupled to the second optical fiber coupler, and the split fluorescence signal is coupled to the third optical fiber coupler.

Preferably, the light splitting component includes a dichroic mirror or a light splitter, if the light splitting component is the dichroic mirror, the dichroic mirror transmits one of the side scattering signal and the fluorescence signal, and reflects the other of the side scattering signal and the fluorescence signal, and if the light splitting component is the light splitter, the light splitter is a transmission grating.

Preferably, the sample analyzer further comprises a second optical assembly disposed between the flow chamber and the light splitting assembly and coaxially disposed with the flow chamber for converging the side scatter signal and the fluorescence signal, so that the converged side scatter signal and the converged fluorescence signal pass through the light splitting assembly.

Preferably, at least two of the first photodetector, the second photodetector, and the third photodetector are integrated in the same signal processor.

A second aspect of embodiments of the present application provides a particle measurement apparatus, including:

a front light assembly for providing an incident light beam for illuminating the detected particles;

a flow chamber for providing a place where the detected particles are irradiated by the incident light beam when the detected particles flow through the sheath fluid;

the optical fiber coupler comprises an incident end, an optical fiber and an emergent end, wherein the incident end is used for coupling an optical signal generated after the incident beam interacts with the detected particles in the flow chamber into the optical fiber and enabling the optical signal to reach the emergent end through the optical fiber;

and the photoelectric detector is used for detecting the optical signal of the emergent end and outputting an electric signal.

Preferably, the particle measurement apparatus further includes: a diaphragm disposed between the flow cell and the fiber coupler;

the diaphragm is used for filtering the ambient light signal and screening the light signal so as to enable the light signal to enter the incident end of the optical fiber coupler, wherein the diaphragm is arranged at the focal position of the light signal.

According to the technical solution provided by the utility model, the embodiment of the utility model has the following advantage:

in the embodiment of the application, can transmit the scattered signal to the photoelectric detector through the optical fiber coupler, because the optic fibre in the optical fiber coupler can twine optic fibre under the prerequisite that does not take place to damage, thereby the optical path before scattered signal transmission to the photoelectric detector has been shortened, thereby optical system's miniaturization in the sample analysis appearance has been realized, and optic fibre is a confined light path transmission system, so when transmitting the scattered signal through the optical fiber coupler, can also further reduce the interference of ambient light in the free space, promote the SNR of scattered signal.

Drawings

FIG. 1A is a schematic diagram of a sample analyzer according to an embodiment of the present disclosure;

FIG. 1B is a schematic diagram of a detecting unit of a sample analyzer according to an embodiment of the present application;

FIG. 2 is a schematic view of another embodiment of a detecting unit of the sample analyzer of the present application;

FIG. 3 is a schematic view of another embodiment of a detecting unit of the sample analyzer of the present application;

FIG. 4 is a schematic view of another embodiment of a detecting unit of the sample analyzer of the present application;

FIG. 5A is a schematic view of another embodiment of a detecting unit of the sample analyzer of the present application;

FIG. 5B is a schematic view of another embodiment of a test device of the sample analyzer of the present application;

FIG. 6 is a schematic view showing another structure of a detecting unit of the sample analyzer according to the embodiment of the present application;

FIG. 7 is a schematic view showing the structure of a particle measuring apparatus according to an embodiment of the present application;

FIG. 8 is a schematic view showing another structure of a particle measuring apparatus according to an embodiment of the present application.

The reference numbers are as follows:

the device comprises a sampling device 101, a reagent device 102, a sample preparation device 103, a detection device 104, a front light assembly 1041, a flow chamber 1042, an optical fiber coupler 1043, a photodetector 1044, a laser generator 10411, a collimating lens 10412, a cylindrical mirror 10413, a cylindrical mirror 10414, a first optical fiber coupler 10431, a second optical fiber coupler 10432, a third optical fiber coupler 10433, a first photodetector 10441, a second photodetector 10442, a third photodetector 10443, a first diaphragm 1045, a first optical assembly 1046, a second optical assembly 1047, a dichroic mirror 1048, a transmission grating 1049, a filter 1050 and a second diaphragm 1051.

Detailed Description

The embodiment of the application provides a sample analyzer and a particle measuring device, which are used for reducing the volume of an optical system in the sample analyzer and the particle measuring device and improving the signal to noise ratio of a measured optical signal.

In order to make the technical solution of the present invention better understood, the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall belong to the protection scope of the present invention.

The terms "first," "second," "third," "fourth," and the like in the description and in the claims, as well as in the drawings, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It will be appreciated that the data so used may be interchanged under appropriate circumstances such that the embodiments described herein may be implemented in other sequences than those illustrated or described herein. Moreover, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

For ease of understanding, the sample analyzer in the present embodiment is described below, with reference to fig. 1A and 1B, and the sample analyzer in fig. 1A includes: a sampling device 101, a reagent device 102, a sample preparation device 103, and a detection device 104, wherein the detection device 104 includes: a front light assembly 1041, a flow chamber 1042, a fiber optic coupler 1043, and a photodetector 1044.

Sampling device 101 is used for gathering the sample that awaits measuring, like the blood sample, and is specific, and sampling device 101 includes sampling needle, power device to and connect sampling needle, power device's collection pipeline, power device is used for driving the sampling needle and gathers the sample that awaits measuring. Further, after the sampling device 101 collects the sample to be tested, the sample to be tested can be sucked to be provided to the sample preparation device 103.

The reagent device 102 is used for carrying a reagent in a test solution, and the reagent is provided to the sample preparation device 103 after being drawn. The reagent device 102 may comprise a reagent carrier and a reagent dispensing mechanism in some embodiments. The reagent carrying part is used for carrying a reagent. In one embodiment, the reagent carrier may be a reagent disk, the reagent disk is configured in a disk-shaped structure and has a plurality of positions for carrying reagent containers, and the reagent carrier can rotate and drive the reagent containers carried by the reagent carrier to rotate, so as to rotate the reagent containers to a specific position, for example, a position where a reagent is sucked by the reagent dispensing mechanism. The number of reagent carrying parts may be one or more. The reagent dispensing mechanism is used for sucking and discharging a reagent into a reaction cell to which the reagent is added. In one embodiment, the reagent dispensing mechanism may include a reagent needle that performs a two-dimensional or three-dimensional motion in space by a two-dimensional or three-dimensional driving mechanism, so that the reagent needle can move to aspirate a reagent carried by the reagent carrying member and to the sample preparation device 103 to which the reagent is to be added and discharge the reagent to the sample preparation device 103.

A sample preparation device 103 for receiving the sample to be tested in the sampling device 101 and the reagent in the reagent device 102 to prepare the sample to be tested and the reagent into a blood sample to be tested;

the detecting device 104 is used for performing specific item detection on the blood sample to obtain a detection result, such as detecting the blood sample to obtain different cells (white blood cells, red blood cells) in the blood sample to be detected, and counting the different cells.

The detection device 104 includes a front light assembly 1041, a flow chamber 1042, a fiber coupler 1043, and a photodetector 1044, and fig. 1B shows a schematic structural diagram of the detection device for ease of understanding.

A front light assembly 1041 for providing an incident light beam to irradiate the blood sample to be measured. In some embodiments, the front light assembly 1041 includes a laser generator 10411, which may be a laser diode, and a collimating lens 10412 disposed along the optical path of the laser, and two cylindrical mirrors 10413 and 10414 disposed perpendicular to each other.

Typically, the flow chamber 1042 is a transparent chamber having an aperture through which the blood sample to be tested flows under the entrapment of the sheath fluid. In this embodiment, the flow chamber 1042 is disposed between the cylindrical mirror 10414 and the optical fiber coupler 1043, and after the collimating lens and the two cylindrical mirrors disposed perpendicular to each other collect and converge the laser light, a light spot is formed at the flow chamber 1042. In order to reduce the space volume occupied by the optical system in the sample analyzer, in the embodiment of the present application, the scattering signal is coupled into the incident end of the optical fiber coupler through the optical fiber coupler 1043, and is transmitted to the exit end of the optical fiber coupler through the optical fiber between the incident end and the exit end, so that the photoelectric detector 1044 detects the scattering signal at the exit end, and outputs an electrical signal.

Specifically, the optical fiber coupler 1043 includes an incident end, an optical fiber, and an exit end, where the incident end is configured to couple a scattering signal generated after an incident beam interacts with the blood sample to be detected in the flow chamber 1042 into the optical fiber, and enable the scattering signal to reach the exit end through the optical fiber;

and the photoelectric detector 1044 is used for detecting the scattering signal at the emergent end and outputting an electrical signal.

In the embodiment of the application, can transmit the scattered signal to the photoelectric detector through the optical fiber coupler, because the optic fibre in the optical fiber coupler can twine optic fibre under the prerequisite that does not take place to damage, thereby optical path between scattered signal transmission to the photoelectric detector has been shortened, thereby optical system's miniaturization in the sample analysis appearance has been realized, and optic fibre is a confined light path transmission system, so when transmitting the scattered signal through the optical fiber coupler, can also further reduce the interference of ambient light in the free space, promote the SNR of scattered signal.

Based on the scatter signal generated in fig. 1B, wherein the scatter signal may be at least one of a forward scatter signal, a side scatter signal, and a fluorescence signal.

Specifically, in some embodiments, the forward scatter signal is a forward scatter signal at a first angle, and in other embodiments, the forward scatter signal is a forward scatter signal at a second angle, wherein the first angle of the scatter signal is in a range of 1 to 10 degrees from the optical axis of the incident light beam; the second angle of the scattered signal is within a range of 20 to 45 degrees from the optical axis of the incident beam.

Further, the side scattering signal in the embodiment of the present application is a scattering signal having an angle of 70 to 110 degrees with the optical axis of the incident light beam.

It should be noted that, in the embodiment of the present application, a single laser generator 10411 is taken as an example to describe the detection device, and in practical cases, a plurality of laser generators may be further included, wherein the plurality of laser generators are arranged in different directions to emit incident light beams in a plurality of different directions, and when the detection device includes a plurality of laser generators, the incident light beams in the present application refer to the incident light beams emitted from the plurality of laser generators after being finally combined and then emitted into the flow chamber.

The following description is given of the optical system in the detection device of the sample analyzer when the scattering signal is a forward scattering signal:

referring to fig. 2, if the scattered signal is a forward scattered signal, the detecting device 104 in the sample analyzer includes: the blood flow measuring device comprises a front light assembly 1041, a flow chamber 1042, an optical fiber coupler 1043, a photodetector 1044, and a first diaphragm 1045 disposed between the flow chamber 1042 and the optical fiber coupler 1043, wherein the first diaphragm 1045 is configured to filter an outgoing light beam that does not interact with a blood sample to be measured when an incident light beam transmits through the flow chamber 1042, and screen out a forward scattering signal generated after the incident light beam interacts with the blood sample to be measured when the incident light beam transmits through the flow chamber, so that the forward scattering signal is incident into the optical fiber coupler 1043.

Specifically, the first diaphragm 1045 has a first semicircular opening and a second semicircular opening, and a light shielding member is disposed between the first semicircular opening and the second semicircular opening, wherein the forward scattering signal passes through the first semicircular opening and the second semicircular opening, and the emergent light beam that does not interact with the blood sample to be measured is blocked by the light shielding member.

In order to make the first diaphragm 1045 better block the outgoing light beam which does not interact with the sample to be measured when the incident light beam transmits through the flow chamber 1042, in the embodiment of the present application, the first diaphragm 1045 is disposed at the focal position of the outgoing light beam which does not interact with the blood to be measured when the incident light beam transmits through the flow chamber 1042. The cylindrical mirrors 10413 and 10414 in the front light assembly 1041 can converge the laser beam in two directions, wherein one cylindrical mirror is used for focusing the focal point of the incident beam in the flow chamber 1042, and the other cylindrical mirror is used for focusing the focal point of the incident beam at the first position behind the flow chamber 1042, and the first position is the focal point of the outgoing beam which does not interact with the blood sample to be measured when the incident beam passes through the flow chamber 1042.

In the embodiment of the application, the diaphragm is arranged in front of the optical fiber coupler, so that when the diaphragm transmits an incident light beam through the flow chamber, the emergent light beam which does not interact with the blood sample to be detected is filtered, and the signal-to-noise ratio of a forward scattering signal is further improved.

Based on the embodiment shown in fig. 2, the forward scattering signal will often have a certain divergence angle before being coupled into the fiber coupler 1043, and in order to couple more scattering signals into the fiber coupler 1043, referring to fig. 3, a first optical element 1046 may be further disposed between the flow chamber 1042 and the first diaphragm 1045 for converging the forward scattering signal and the outgoing light beam that does not interact with the blood sample to be measured when the incoming light beam passes through the flow chamber 1042.

Specifically, the first optical assembly 1046 may be a single convex lens, a double convex lens, a spherical lens, or a combination of multiple convex lenses, etc., that is, as long as the first optical assembly can converge the forward scattering signal, and the form of the first optical assembly is not particularly limited herein.

If the first optical assembly 1046 is disposed between the flow chamber 1042 and the first diaphragm 1045, the position of the first diaphragm 1045 in fig. 3 is also changed compared to the position in fig. 2, specifically, in the detection apparatus of the sample analyzer shown in fig. 3, the first diaphragm 1045 is disposed at the focal position of the first optical assembly 1046, so that the converged forward scattering signal passes through the first semicircular opening and the second semicircular opening, and the converged outgoing light beam which does not interact with the blood sample to be measured is blocked by the light blocking member.

Based on the embodiment described in fig. 3, if the scattering signal further includes a side scattering signal, please refer to fig. 4, the fiber coupler 1043 includes a first fiber coupler 10431 and a second fiber coupler 10432, and the photodetector 1044 includes a first photodetector 10441 and a second photodetector 10442; the first fiber coupler 10431 is configured to couple the forward scattering signal, so that the first photodetector 10441 detects the forward scattering signal; the second fiber coupler 10432 is used for coupling the side scatter signal so that the second photodetector 10442 detects the side scatter signal.

Specifically, in order to detect stronger side scatter signals, i.e. to make the signal-to-noise ratio of the side scatter signals stronger, a second optical assembly 1047 may be further disposed between the flow chamber 1042 and the second fiber coupler 10432 for converging the side scatter signals, so that the converged side scatter signals are coupled into the second fiber coupler 10432.

The second optical assembly 1047 may be a single convex lens, a double convex lens, a spherical lens, or a combination of multiple convex lenses, that is, as long as the second optical assembly can converge the side scattering signal, and the form of the second optical assembly is not particularly limited here.

In the embodiment of the application, when the scattering signal includes a forward scattering signal and a side scattering signal, the forward scattering signal and the side scattering signal are coupled and transmitted through the first optical fiber coupler and the second optical fiber coupler respectively, so that the volume of an optical system in the sample analyzer is further reduced, meanwhile, the interference of ambient light in a free space is further reduced through the transmission mode of the optical fibers, and the signal-to-noise ratio of the forward scattering signal and the side scattering signal is improved.

In the embodiment shown in fig. 4, if the scattering signal further includes a fluorescence signal, please refer to the detecting apparatus of the sample analyzer shown in fig. 5A or fig. 5B, wherein the fiber coupler 1043 includes a first fiber coupler 10431, a second fiber coupler 10432 and a third fiber coupler 10433, and the photodetector 1044 includes a first photodetector 10441, a second photodetector 10442 and a third photodetector 10443, wherein the first fiber coupler 10431 is used for coupling the forward scattering signal, so that the first photodetector 10441 detects the forward scattering signal; the second fiber coupler 10432 is used for coupling the side scattering signal, so that the second photodetector 10442 detects the side scattering signal; the third fiber coupler 10433 is used for coupling the fluorescence signal, so that the third photodetector 10443 detects the fluorescence signal.

Specifically, the third photodetector 10443 in the embodiment of fig. 5A and 5B is an enhanced photodetector, such as a photomultiplier tube, because the fluorescence signal is weaker than the forward scatter signal and the side scatter signal.

Further, if the side scatter signal is a scatter signal at the same angle as the fluorescence signal, the sample analyzer further comprises a light splitting assembly disposed laterally coaxially with the flow chamber 1042.

For convenience of illustration, in the embodiment of fig. 5A and 5B, the side scattering signal and the fluorescence signal are both 90 ° in the lateral direction, in order to split the side scattering signal and the fluorescence signal at 90 ° in the lateral direction, the light splitting component may be a dichroic mirror 1048 in fig. 5A or a light splitter in fig. 5B, if the light splitting component is the dichroic mirror 1048 in fig. 5A, the dichroic mirror 1048 is used to transmit one of the side scattering signal and the fluorescence signal and reflect the other of the side scattering signal and the fluorescence signal, and if the light splitting component is the light splitter in fig. 5B, the light splitter is a transmission grating 1049.

In the embodiment of fig. 5A and 5B, the detection device in the sample analyzer for collecting the forward scattering signal, the side scattering signal and the fluorescence signal is described in detail, the forward scattering signal is coupled through the first fiber coupler, the side scattering signal is coupled through the second fiber coupler, and the fluorescence scattering signal is coupled through the third fiber coupler, so that the size of the optical system in the sample analyzer is further reduced, meanwhile, the interference of the ambient light in the free space is further reduced by the transmission mode of the optical fiber, and the signal-to-noise ratio of the forward scattering signal, the side scattering signal and the fluorescence signal is improved.

Based on the embodiment described in fig. 5A or fig. 5B, in order to enhance the detection of the fluorescence signal by the third photodetector 10443, a filter 1050 may be further disposed between the exit end of the third fiber coupler 10433 and the third photodetector 10443, so as to filter out a stray light signal except the fluorescence signal, please refer to fig. 6 in particular.

In order to further reduce the volume of the optical system in the sample analyzer for detecting the forward scattering signal, the side scattering signal and the fluorescence signal, at least two of the first photodetector 10441, the second photodetector 10442 and the third photodetector 10443 may be integrated on the same signal processor, so as to further reduce the volume of the optical system in the sample analyzer and improve the integration level of the optical system in the sample analyzer.

In the schematic diagram of fig. 6, the first photodetector 10441, the second photodetector 10442, and the third photodetector 10443 are simultaneously integrated on the same signal processor (e.g., signal processing board card), so that the volume of the detection apparatus is further miniaturized.

As described above in detail with respect to the sample analyzer in the embodiment of the present application, the following description will be made of a particle measurement apparatus in the embodiment of the present application, with reference to fig. 7, where the particle measurement apparatus in fig. 7 includes: a front light assembly 1041, a flow chamber 1042, a fiber optic coupler 1043, and a photodetector 1044.

Specifically, the front light assembly 1041 is used for providing an incident light beam for irradiating detected particles, and in some embodiments, the front light assembly 1041 includes a laser generator 10411, a collimating lens 10412 disposed along a laser path, two cylindrical mirrors 10413 and 10414 disposed perpendicular to each other, and the laser generator 10411 may be a laser diode. The collimating lens 10412 and the two cylindrical mirrors 10413 and 10414, which are vertically disposed, collect and converge the laser light, and then form a light spot at the flow chamber 1042. The light spot interacts with the detected particles in flow chamber 1042 to produce an optical signal.

The flow chamber 1042 is a transparent chamber with an aperture for the sheath fluid to entrain the particles to be detected to pass through the aperture to provide a location where the particles to be detected are illuminated by the incident light beam.

The optical fiber coupler 1043 includes an incident end, an optical fiber, and an exit end, wherein the incident end is configured to couple an optical signal generated after an incident beam interacts with detected particles in the flow chamber 1042 into the optical fiber, and enable the optical signal to reach the exit end through the optical fiber.

The photodetector 1044 is configured to detect the optical signal at the emitting end and output an electrical signal.

It should be noted that the optical signal in the embodiment of the present application includes not only a scattered light signal, but also a transmitted light intensity signal after an incident light beam interacts with a sample to be detected when the incident light beam penetrates through the flow cell, so as to be used for measuring particles (urinary sediment, or specific reactive protein) to be detected in other samples (such as urine samples or serum samples).

In the embodiment of the application, optical signal transmits to photoelectric detector through optical fiber coupler, because optical fiber among the optical fiber coupler can twine optical fiber under the prerequisite that does not take place to damage, thereby optical signal transmission to photoelectric detector between the optical path has been shortened, optical system's miniaturization among the particle measuring device has been realized, and optical fiber is a confined light path transmission system, so when transmitting optical signal through optical fiber coupler, can also further reduce the interference of ambient light among the free space, promote optical signal's SNR.

Further, when the sample in fig. 7 is a blood sample and the optical signal is a scattered light signal, the optical system in the particle measurement device is similar to the optical system in the sample analyzer detection device in the embodiments of fig. 1 to 6, and the description thereof is omitted here.

When the sample in fig. 7 is other samples (such as urine sample or serum sample), the detected particle is urinary sediment or specific reactive protein, and the optical signal is a transmitted light intensity signal, a second diaphragm 1051 may be further disposed between the flow chamber 1042 and the optical fiber coupler 1043 in fig. 7, as shown in fig. 8.

The second stop 1051 is disposed at the focal point of the transmitted light, so as to allow the transmitted light signal to pass through the second stop 1051, and filter the ambient light signal, so as to enhance the signal-to-noise ratio of the transmitted light intensity signal.

The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (18)

1. A sample analyzer, comprising:

a sampling device for collecting a blood sample;

the reagent device is used for bearing a reagent;

the sample preparation device is used for mixing the blood sample and the reagent in the reaction pool to prepare a blood sample to be detected;

the detection device is used for detecting specific items of the blood sample to be detected so as to obtain a detection result;

wherein the detection device comprises: the front light assembly is used for providing an incident beam for irradiating the blood sample to be detected;

the flow chamber is used for providing a place where the blood sample to be detected is irradiated by the incident light beam when the blood sample to be detected flows through the sheath fluid in a wrapping mode;

the optical fiber coupler comprises an incident end, an optical fiber and an emergent end, wherein the incident end is used for coupling a scattering signal generated after the incident beam interacts with the blood sample to be detected in the flow chamber into the optical fiber and enabling the scattering signal to reach the emergent end through the optical fiber;

and the photoelectric detector is used for detecting the scattering signal of the emergent end and outputting an electric signal.

2. The sample analyzer of claim 1, wherein the scatter signal includes at least one of a forward scatter signal, a side scatter signal, and a fluorescence signal.

3. The sample analyzer of claim 2, wherein the forward scatter signal includes at least one of a first angle forward scatter signal and a second angle forward scatter signal;

wherein the forward scatter signal of the first angle comprises: a scattered signal with an included angle of 1 to 10 degrees with the optical axis of the incident beam;

the forward scatter signal of the second angle comprises: and the included angle between the optical axis of the incident light beam and the scattering signal is in the range of 20-45 degrees.

4. The sample analyzer of claim 2, wherein the side scatter signal comprises: and the included angle between the optical axis of the incident light beam and the scattered signal is in the range of 70 to 110 degrees.

5. The sample analyzer of claim 2, wherein the scatter signal comprises the forward scatter signal, the sample analyzer further comprising:

the diaphragm is used for filtering emergent light beams which do not interact with the blood sample to be detected when the incident light beams penetrate through the flow chamber, and screening forward scattering signals generated after the incident light beams interact with the blood sample to be detected when the incident light beams penetrate through the flow chamber, so that the forward scattering signals are emitted into the optical fiber coupler.

6. The sample analyzer of claim 5, wherein the aperture has a first semi-circular opening and a second semi-circular opening with a light shield disposed therebetween, wherein the forward scattered signal passes through the first semi-circular opening and the second semi-circular opening, and the emergent beam that has not interacted with the blood sample to be tested is blocked by the light shield.

7. The sample analyzer as claimed in claim 5, wherein the diaphragm is disposed at a focal position of the emergent beam that does not interact with the blood sample to be measured.

8. The sample analyzer of claim 5, further comprising a first optical assembly disposed between the flow cell and the diaphragm, wherein the first optical assembly is configured to converge the forward scatter signal and the exit beam that has not interacted with the blood sample.

9. The sample analyzer of claim 8, wherein the aperture is disposed at a focal position of the first optical assembly.

10. The sample analyzer of any of claims 5 to 9, wherein the scattered signal further comprises the side scattered signal, the fiber coupler comprises a first fiber coupler and a second fiber coupler, and the photodetector comprises a first photodetector and a second photodetector;

wherein the first fiber coupler is configured to couple the forward scatter signal such that the first photodetector detects the forward scatter signal;

the second optical fiber coupler is used for coupling the side scattering signals so that the second photoelectric detector can detect the side scattering signals.

11. The sample analyzer of claim 10 wherein the scattered signal further comprises the fluorescent signal, the fiber coupler further comprises a third fiber coupler, and the photodetector further comprises a third photodetector;

wherein the third fiber coupler is configured to couple the fluorescence signal, so that the third photodetector detects the fluorescence signal.

12. The sample analyzer of claim 11 further comprising a filter disposed between the exit end of the third fiber coupler and the third photodetector for filtering stray light signals other than the fluorescence signal.

13. The sample analyzer of claim 11, further comprising a light splitting assembly, wherein,

the light splitting assembly and the flow chamber are arranged coaxially in the lateral direction, and the light splitting assembly is used for splitting the lateral scattering signal and the fluorescence signal, wherein the split lateral scattering signal is coupled to the second optical fiber coupler, and the split fluorescence signal is coupled to the third optical fiber coupler.

14. The sample analyzer of claim 13 wherein the light splitting component comprises a dichroic mirror or a light splitter, wherein if the light splitting component is the dichroic mirror, the dichroic mirror transmits one of the side scatter signal and the fluorescence signal and reflects the other of the side scatter signal and the fluorescence signal, and wherein if the light splitting component is the light splitter, the light splitter is a transmission grating.

15. The sample analyzer of claim 13, further comprising a second optical assembly disposed between the flow chamber and the light splitting assembly and coaxial with the flow chamber for converging the side scatter signal and the fluorescence signal such that the converged side scatter signal and fluorescence signal pass through the light splitting assembly.

16. The sample analyzer of claim 11 wherein at least two of the first photodetector, the second photodetector, and the third photodetector are integrated into the same signal processor.

17. A particle measurement device, comprising:

a front light assembly for providing an incident light beam for illuminating the detected particles;

a flow chamber for providing a place where the detected particles are irradiated by the incident light beam when the detected particles flow through the sheath fluid;

the optical fiber coupler comprises an incident end, an optical fiber and an emergent end, wherein the incident end is used for coupling an optical signal generated after the incident beam interacts with the detected particles in the flow chamber into the optical fiber and enabling the optical signal to reach the emergent end through the optical fiber;

and the photoelectric detector is used for detecting the optical signal of the emergent end and outputting an electric signal.

18. The particle measurement apparatus according to claim 17, further comprising: a diaphragm disposed between the flow cell and the fiber coupler;

the diaphragm is used for filtering the ambient light signal and screening the light signal so as to enable the light signal to enter the incident end of the optical fiber coupler, wherein the diaphragm is arranged at the focal position of the light signal.

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