CN206045474U - The continuous desalination of automatization or decoloration device - Google Patents
The continuous desalination of automatization or decoloration device Download PDFInfo
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- CN206045474U CN206045474U CN201621069898.9U CN201621069898U CN206045474U CN 206045474 U CN206045474 U CN 206045474U CN 201621069898 U CN201621069898 U CN 201621069898U CN 206045474 U CN206045474 U CN 206045474U
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- electromagnetic valve
- pump
- chromatographic column
- automatization
- liquid
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Abstract
This utility model is related to a kind of continuous desalination of automatization or decoloration device, I entrance of pump is connected with sample bottle, the outlet of pump I is connected with electromagnetic valve II and electromagnetic valve VII respectively by electromagnetic valve I, electromagnetic valve II connects chromatographic column I and electromagnetic valve VI, electromagnetic valve III connects chromatographic column I and detector, the connection receiving flask of electromagnetic valve IV and waste liquid bottle.Electromagnetic valve V connects pure water and organic faciess to pump II, electromagnetic valve II or electromagnetic valve VII are reached by electromagnetic valve VI from the liquid of the conveying of pump II, the liquid flowed out from electromagnetic valve VII reaches chromatographic column II, the liquid flowed out from chromatographic column II reaches detector or electromagnetic valve Ⅸ by electromagnetic valve VIII, and electromagnetic valve Ⅸ connects waste liquid bottle and sample bottle respectively.Advantage of the present utility model：Serialization, the automation mechanized operation of desalination or decolouring in commercial production can be realized, sample treatment speed is greatly speeded up, and is saved human cost, and can be combined with the chromatographic column of various sizes.
Description
Technical field
This utility model is related to desalination or decolouring technology field, specifically, is a kind of continuous desalination of automatization or decolouring
Device.
Background technology
With the development and the propulsion of genetic engineering industry of pharmaceuticals industry, liquid chromatography exists as a kind of separation method
Effect in Sample Purification on Single increasingly looms large.Liquid-phase chromatographic column is to adsorb, distribute, ion attraction, exclusion, the function such as affine can
The application effects such as desalination, separation, refined and decolouring are realized, so it is widely used in electric power, chemical industry, metallurgy, medicine, food
With the department such as atomic energy.In the last few years, liquid chromatograph was developed rapidly in medical and health cause, such as in drug manufacture,
It is widely used in extraction of desalination, adsorbing separation, purification, decolouring, neutralization and Chinese herbal medicine effective ingredientses of medicament etc..Cause
There is efficient characteristic for preparative liquid chromatography, even if so complicated and difficult point can also be separated using single-column preparing chromatography system
From component of mixture, but, as there is chromatographic column certain static state to combine carrying capacity, so as to limit the applied sample amount of sample,
In conjunction with the time consumed by sample penetration, desalination, eluting and column regeneration, this largely reduces people in practice
In, especially work efficiency in the industrial production.For problem above, this utility model devises a kind of device, Ke Yishi
The serialization of desalination or decolouring in existing commercial production, automation mechanized operation, the characteristics of with simple and practical, high efficiency and time conservation.
Utility model content
The purpose of this utility model is to overcome existing desalination or decolouring technology, complex operation step, need it is great
Manpower keeps on the go and the not high shortcoming of production efficiency, there is provided a kind of continuous desalination of automatization or decoloration device and its method,
The characteristics of with continuous operation, automatic operating, saving manpower, time-saving and efficiency.
The purpose of this utility model is achieved through the following technical solutions：
A kind of continuous desalination of automatization or decoloration device, it is characterised in that
I entrance of pump is connected with sample bottle, and the outlet of pump I is connected with electromagnetic valve II and electromagnetic valve VII respectively by electromagnetic valve I,
Electromagnetic valve II connects chromatographic column I and electromagnetic valve VI, the connection chromatographic column I of electromagnetic valve III and detector, the connection receiving flask of electromagnetic valve IV
And waste liquid bottle.Electromagnetic valve V connects pure water and organic faciess to pump II, reaches electromagnetism by electromagnetic valve VI from the liquid of the conveying of pump II
Valve II or electromagnetic valve VII, the liquid flowed out from electromagnetic valve VII reach chromatographic column II, and the liquid flowed out from chromatographic column II passes through electromagnetism
Valve VIII reaches detector or electromagnetic valve Ⅸ, and electromagnetic valve Ⅸ connects waste liquid bottle and sample bottle respectively.
The pump is the one kind in several fluid pumps such as efficient liquid-phase chromatographic pump, preparative liquid chromatography pump, peristaltic pump；
Described efficient liquid-phase chromatographic pump refers to range of flow in 0.1～5mL/min；
Described preparative liquid chromatography pump causes range of flow in 1～1000mL/min；
Described peristaltic pump refers to range of flow in 0.01～1mL/min；
The electromagnetic valve is three-way magnetic valve；
The chromatographic column is analysis liquid-phase chromatographic column, semi-preparative liquid chromatography post, middle compacting for liquid-phase chromatographic column, high compacting
Standby liquid-phase chromatographic column, axial compression columns (DAC) and industrial preparation chromatographic column；
The analysis chromatographic column is internal diameter in 2.0～4.6mm；
The semi-preparative liquid chromatography column diameter is in 10～25mm；
Medium pressure preparation scale chromatograph column internal diameter is in 15～150mm；
The high pressure preparative liquid chromatography column internal diameter is in 4.6～30mm；
The liquid chromatographic packing materials are C18, C8, C4, phenyl, pentafluorophenyl group, penta-bromophenyl, graphitized carbon, macropore suction
Attached resin etc..
The detector is UV-vis detector, differential refraction detector, evaporative light scattering detector, Mass Spectrometer Method
Device, circular dichroism detector etc.；
Described continuous eluting is that the Vavle switching technology controlled by computer realizes the used in parallel of two liquid-phase chromatographic columns
Realize.
Compared with prior art, good effect of the present utility model is：In overcoming existing desalination or decolouring technology, operation step
Rapid loaded down with trivial details, the great manpower of needs keeps on the go and the not high shortcoming of production efficiency, realizes desalination or decolouring in commercial production
Serialization, automation mechanized operation, greatly speed up sample treatment speed, and save human cost, and can be with various sizes
Chromatographic column combination.
Description of the drawings
Fig. 1 connection system structure charts of the present utility model；
Fig. 2 this utility model is embodied as continuous desalination or decolouring operating procedure figure；
Fig. 3 this utility model is embodied as continuous desalination or decolouring operating procedure figure；
Fig. 4 this utility model is embodied as continuous desalination or decolouring operating procedure figure；
Fig. 5 this utility model is embodied as continuous desalination or decolouring operating procedure figure.
Specific embodiment
The specific embodiment of a kind of continuous desalination of automatization of this utility model presented below or decoloration device.
Embodiment 1：
The eluting of chenodeoxycholic acid
Refer to accompanying drawing 1, I entrance of pump is connected with sample bottle, outlet by electromagnetic valve I respectively with electromagnetic valve II and electromagnetic valve
VII connection, the connection silicagel column I of electromagnetic valve II and electromagnetic valve VI, the connection silicagel column I of electromagnetic valve III and detector, electromagnetic valve IV connect
Receiving flask and waste liquid bottle.Electromagnetic valve V connects pure water and methanol to pump II, is reached from the liquid of the conveying of pump II by electromagnetic valve VI
Electromagnetic valve II or electromagnetic valve VII, the liquid flowed out from electromagnetic valve VII reach silicagel column II, and the liquid flowed out from silicagel column II passes through
Electromagnetic valve VIII reaches detector or electromagnetic valve Ⅸ, and electromagnetic valve Ⅸ connects waste liquid bottle and sample bottle respectively.
The preparatory stage of the present embodiment：
Pump I and pump II：Flash A constant flow pumps (Soochow High Technology Chromatography Co., Ltd.)；Sample：Chenodeoxycholic acid
Aqueous solution, concentration are 2mg/mL；Mobile phase：Ultra-pure water and pure methanol.
Post I and post II：I 5 × 310cm of silicagel column (Soochow High Technology Chromatography Co., Ltd.).
Differential refraction detector (SCIENTIFIC CO., LTD).
The experimental stage of the present embodiment：
The operation of II advance sample introduction of post is finished.
The single cycle operation phase 1:Electromagnetic valve I connects pump I and post I with electromagnetic valve II, carries out loading operation, loading to post I
In 2mL/min, loading time is 8min to flow speed control, and electromagnetic valve IV is switched to waste liquid bottle；Electromagnetic valve V connects pure water bottle, electromagnetism
Valve VI connects pump II and post II with electromagnetic valve VII, and desalination operation is carried out to post II, and flow speed control in 2mL/min, desalination time is
8min, valve 9 are connected to waste liquid bottle, refer to accompanying drawing 2；
The single cycle operation phase 2:Electromagnetic valve I still connects pump I and post I with electromagnetic valve II, proceeds loading behaviour to post I
Make, in 2mL/min, loading time is 2min to loading flow speed control, and valve 4 is communicated to waste liquid bottle；Electromagnetic valve V connects methanol bottle, electricity
Magnet valve VI connects pump II and post II with electromagnetic valve VII, carries out elution action to post II, and flow speed control is in 20mL/min, elution time
For 2min, the connection receiving flask of electromagnetic valve Ⅸ, accompanying drawing 3 is referred to；
The single cycle operation phase 3:Electromagnetic valve I connects pump I and post II with electromagnetic valve VII, carries out loading operation to post II, on
In 2mL/min, loading time is 8min to sample flow speed control, and electromagnetic valve Ⅸ is switched to waste liquid bottle；Electromagnetic valve V connects pure water bottle, electricity
Magnet valve VI connects pump II and post I with electromagnetic valve II, and desalination operation is carried out to post I, and flow speed control in 2mL/min, desalination time is
8min, valve 4 are connected to waste liquid bottle, refer to accompanying drawing 4；
The single cycle operation phase 4：Electromagnetic valve I still connects pump I and post II with electromagnetic valve VII, proceeds loading to post I
Operation, in 2mL/min, loading time is 2min to loading flow speed control, and electromagnetic valve IV is switched to waste liquid bottle；Electromagnetic valve V connects first
Alcohol bottle, electromagnetic valve VI and electromagnetic valve II connect pump II and post I, carry out elution action to post I, and flow speed control is washed in 20mL/min
The de- time is 2min, and electromagnetic valve Ⅸ is communicated to receiving flask, refers to accompanying drawing 5；
With above-mentioned single-cycle run time 20min as a metering unit, system automation continuous service 4h runs
12 single cycles, total sample size are 0.9600g, are weighed as 0.8832g, i.e. CDCA after the recycling of final sample eluting
Acid is 92% except eluting salt overall recovery.
It should be pointed out that for those skilled in the art, without departing from the concept of the premise utility,
Some improvements and modifications can also be made, these improvements and modifications also should be regarded as this new protection domain.
The above is only preferred implementation of the present utility model, it is noted that for the common skill of the art
Art personnel, without departing from the concept of the premise utility, can also make some improvements and modifications, these improvements and modifications
Also should be regarded as in protection domain of the present utility model.
Claims (9)
1. a kind of continuous desalination of automatization or decoloration device, it is characterised in that I entrance of pump is connected with sample bottle, the outlet of pump I lead to
Cross electromagnetic valve I to be connected with electromagnetic valve II and electromagnetic valve VII respectively, electromagnetic valve II connects chromatographic column I and electromagnetic valve VI, electromagnetic valve III
Connection chromatographic column I and detector, the connection receiving flask of electromagnetic valve IV and waste liquid bottle, the connection pure water of electromagnetic valve V and organic faciess are to pump
II, electromagnetic valve II or electromagnetic valve VII are reached by electromagnetic valve VI from the liquid of the conveying of pump II, the liquid flowed out from electromagnetic valve VII to
Up to chromatographic column II, the liquid flowed out from chromatographic column II reaches detector or electromagnetic valve Ⅸ by electromagnetic valve VIII, and electromagnetic valve Ⅸ is distinguished
Connection waste liquid bottle and sample bottle.
2. a kind of continuous desalination of automatization as claimed in claim 1 or decoloration device, it is characterised in that the pump is efficient liquid
One kind in phase chromatogram pump, preparative liquid chromatography pump, peristaltic pump.
3. a kind of continuous desalination of automatization as claimed in claim 2 or decoloration device, it is characterised in that described efficient liquid phase
Chromatogram pump refers to range of flow in 0.1～5mL/min；
Described preparative liquid chromatography pump causes range of flow in 1～1000mL/min；
Described peristaltic pump refers to range of flow in 0.01～1mL/min.
4. a kind of continuous desalination of automatization as claimed in claim 1 or decoloration device, it is characterised in that the electromagnetic valve is three
Three-way electromagnetic valve.
5. a kind of continuous desalination of automatization as claimed in claim 1 or decoloration device, it is characterised in that the chromatographic column for point
Analysis liquid-phase chromatographic column, semi-preparative liquid chromatography post, middle compacting for liquid-phase chromatographic column, high pressure preparative liquid chromatography post, be compressed axially
One kind in chromatographic column DAC and industrial preparation chromatographic column.
6. a kind of continuous desalination of automatization as claimed in claim 5 or decoloration device, it is characterised in that the analysis chromatographic column
It is internal diameter in 2.0～4.6mm；
The semi-preparative liquid chromatography column diameter is in 10～25mm；
Medium pressure preparation scale chromatograph column internal diameter is in 15～150mm；
The high pressure preparative liquid chromatography column internal diameter is in 4.6～30mm.
7. a kind of continuous desalination of automatization as claimed in claim 5 or decoloration device, it is characterised in that the chromatographic column filler
For the one kind in C18, C8, C4, phenyl, pentafluorophenyl group, penta-bromophenyl, graphitized carbon, macroporous adsorbent resin.
8. a kind of continuous desalination of automatization as claimed in claim 1 or decoloration device, it is characterised in that the detector is purple
One kind in outer visible detection device, differential refraction detector, evaporative light scattering detector, mass detector, circular dichroism detector.
9. a kind of continuous desalination of automatization as claimed in claim 1 or decoloration device, it is characterised in that described continuous eluting
It is the realization used in parallel that two liquid-phase chromatographic columns are realized by the Vavle switching technology of computer control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621069898.9U CN206045474U (en) | 2016-09-22 | 2016-09-22 | The continuous desalination of automatization or decoloration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621069898.9U CN206045474U (en) | 2016-09-22 | 2016-09-22 | The continuous desalination of automatization or decoloration device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206045474U true CN206045474U (en) | 2017-03-29 |
Family
ID=58367023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621069898.9U Expired - Fee Related CN206045474U (en) | 2016-09-22 | 2016-09-22 | The continuous desalination of automatization or decoloration device |
Country Status (1)
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| CN (1) | CN206045474U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109224530A (en) * | 2018-10-18 | 2019-01-18 | 东华大学 | A kind of nano-cellulose metal ions trapping column and its preparation and application |
| CN109381890A (en) * | 2017-08-10 | 2019-02-26 | 南京凯通粮食生化研究设计有限公司 | The device and operation method of decoloration deionization while separating mixture |
-
2016
- 2016-09-22 CN CN201621069898.9U patent/CN206045474U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109381890A (en) * | 2017-08-10 | 2019-02-26 | 南京凯通粮食生化研究设计有限公司 | The device and operation method of decoloration deionization while separating mixture |
| CN109224530A (en) * | 2018-10-18 | 2019-01-18 | 东华大学 | A kind of nano-cellulose metal ions trapping column and its preparation and application |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170329 |