CN107132299A - A kind of multichannel pack tomographic system - Google Patents
A kind of multichannel pack tomographic system Download PDFInfo
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- CN107132299A CN107132299A CN201710471112.9A CN201710471112A CN107132299A CN 107132299 A CN107132299 A CN 107132299A CN 201710471112 A CN201710471112 A CN 201710471112A CN 107132299 A CN107132299 A CN 107132299A
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- pack
- multichannel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6034—Construction of the column joining multiple columns
- G01N30/6043—Construction of the column joining multiple columns in parallel
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
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Abstract
The invention discloses a kind of multichannel pack tomographic system, the system is made up of central control system, extract tank, eluting solvent tank, elution media tank, compression pump, multichannel pack chromatogram column system, detector and collecting tank.Wherein, three parts of pack chromatographic column and elution discharge system that multichannel pack chromatogram column system is made up of feed storage tank, ten root chromatogram columns are constituted.The problem of objective monomer product that the present invention can solve that floor space in traditional chromatographic separation technology is big, there is pressure differential and time difference between separation chromatography post, produce more extensive, lack of wisdom and isolate and purify is low.A kind of multichannel pack tomographic system device is provided, available for isolating and purifying for the various bioactivators such as polyphenol, flavones, organic acid, polysaccharide and polypeptide.
Description
Technical field
The present invention relates to a kind of device of intelligent chromatogram column separating purification, belong to chemical field.
Background technology
Current chromatography and its related application technology almost have application in all spectra, and chromatographic technique is in scientific research
With there is very important effect in industrial production, its have separation accuracy height, simple equipments, it is easy to operate the features such as.According to
The different principle of chromatographic separation technology, it not can be applied only for the quantitative analysis and detection of material composition, while can use
Preparative separation and purifying in biological substance, are that industrialized production acquisition high-purity bioactive substance is most important, maximally effective
Separating and purifying technology.But development of the chromatographic separation technology in preparation and commercial scale is opened at present also in the initial development phase
The various chromatographic techniques of hair are respectively provided with certain shortcoming and defect, traditional chromatographic separation technology that floor space is big, separation chromatography
There is pressure differential and time difference between post, produce more extensive, lack of wisdom.Thus develop intelligent, intensive, accurate
Change, it is always desired using the continuous operation chromatographic technique of the overall characteristic such as wide.
Exist this invention addresses floor space in traditional chromatographic separation technology is big, between separation chromatography post pressure differential and when
Between it is poor, the problem of produce more extensive, lack of wisdom and the low objective monomer product that isolates and purifies.A kind of multichannel is provided
Pack tomographic system device, the separation available for various bioactivators such as polyphenol, flavones, organic acid, polysaccharide and polypeptides is pure
Change.
The content of the invention
Exist the invention aims to solve in traditional chromatographic separation technology that floor space is big, between separation chromatography post
The problem of pressure differential and time difference, low objective monomer product for producing more extensive, lack of wisdom and isolating and purifying.There is provided
A kind of multichannel pack tomographic system device, available for the multiple biological activities thing such as polyphenol, flavones, organic acid, polysaccharide and polypeptide
Matter is isolated and purified.Multichannel pack tomographic system device of the present invention, it include central control system, extract tank,
Eluting solvent tank, elution media tank, compression pump, multichannel pack chromatogram column system, detector and collecting tank；Collecting tank 1,2 and 3
Height h4 and diameter d1 be 1.13m.
Multichannel pack chromatogram column system includes pack chromatographic column and the elution row that feed storage tank, ten root chromatogram columns are constituted
Go out system.There are water inlet, charging aperture and wide-mouth form in the top of feed storage tank, and media outlet, feed storage tank are arranged at its bottom
Internal and ten root chromatogram column UNICOMs.The height h1 of feed storage tank is 1.5 m；
The height h2 of ten root chromatogram columns is 3m, and the diameter d2 per root chromatogram column is 30cm, and the bottom per root chromatogram column is provided with 80
Purpose liner plate；
The elution discharge port system of multichannel pack chromatogram column system is in funnel-form, and height h3 is 15cm, and ten root chromatogram columns go out
Liquid mouthful is communicated therewith, and elution discharge port system can be dismantled, and is had recoil valve, waste liquid dump valve on elution discharge port system and is washed
De- liquid dump valve；
Extract tank, eluting solvent tank and elution media tank are set up in parallel, extract tank, eluting solvent tank and elution media tank
Upper port receives return valve by one respectively and is connected with the another port of return duct；
The lower port of extract tank, eluting solvent tank and elution media tank passes through a discharge valve and feed pipe respectively
A port is connected, and compression pump is provided with the feed pipe.
Collecting tank has a n, and n is natural number, and n=3 ~ 10, and the upper port of each collecting tank elutes outlet phase respectively
Even, and by central control system elution outlet is controlled to the switch of each collecting tank；
The lower port of collecting tank is connected by a discharge valve with solvent recovering system respectively, is provided with the recovery system
Compression pump.
Based on above-mentioned multichannel pack chromatogram column system device, it is characterised in that this method is involved the steps of：
Step one so that ten chromatographic columns in the multichannel pack chromatogram column system are in paralleling model, by elution media
The medium that isolates and purifies in tank is transferred in feed storage tank by compression pump, is subsequently filled into each chromatographic column；
Step 2, the material in extract tank is transferred in feed storage tank by compression pump, is then loaded to each chromatogram
In post；
Step 3, the eluting solvent in eluting solvent tank is transferred in feed storage tank by compression pump, then to each color
Post is composed elute simultaneously；
Step 4, detector carries out automatic classification by carrying out analysis detection to eluent, to the biological substance afforded and received
Collection, it is mutually deserved to enter 1 ~ n of collecting tank；
Step 5, after elution terminates, opens the medium outlet on the recoil valve and feed storage tank on elution discharge port system,
Elution media in all chromatographic columns can be recoiled out.
Advantages of the present invention：
1st, isolating and purifying available for various bioactivators such as polyphenol, flavones, organic acid, polysaccharide and polypeptides；
2nd, it ensure that the synchronization of multiple chromatographic column pressure consistencies and charging elution；
3rd, integration is related to, and greatlys save space and floor space；
4th, solid-phase media is changed convenient and swift；
5th, the intellectuality and automation of chromatographic separation and purification are realized substantially.
Brief description of the drawings
Fig. 1 is the structural representation of multichannel pack tomographic system device of the present invention；
Fig. 2 is the front cross-sectional view of multichannel pack thin layer chromatography post of the present invention；
Fig. 3 is multichannel pack tomographic system central control system schematic diagram of the present invention.
Embodiment
Technical scheme is further described below in conjunction with the accompanying drawings, but is not limited thereto, it is every to this
Inventive technique scheme is modified or equivalent substitution, without departing from the spirit and scope of technical solution of the present invention, all should be covered
In protection scope of the present invention.
Embodiment 1
As shown in figure 1, the multichannel pack tomographic system that the present invention is provided is by 1 extract holding vessel, 2 eluting solvent holding vessels, 3
Elution media holding vessel, 4 compression pumps, 5 central control systems, 8 multichannel pack chromatogram column systems, 9 detectors, 11 collecting tanks 3,
12 collecting tanks 2 and the grade part of 13 collecting tank 1 composition；The height h4 and diameter d1 of collecting tank 1,2 and 3 are 1m ~ 4m.
As shown in figure 1, the pack chromatogram that multichannel pack chromatogram column system is made up of 6 feed storage tanks, 70 root chromatogram columns
Post and 10 three parts of elution discharge system are constituted, as shown in Fig. 2 whole multichannel pack chromatogram column system includes 10 chromatograms
The medium of filling can be changed inside post, each chromatographic column, and 2 is signified for solid area, 10 chromatographic columns be an entirety from
And form the multichannel pack chromatogram column system of a cylinder；The relevant parameter of each chromatographic column：The cm of diameter 20 ~ 60, it is long
Degree：1m ~ 4m, capacity 8L ~ 20L；
F1, F2 and F3 as shown in Figure 1 are respectively 3 elution media holding vessels, 2 eluting solvent holding vessels and 1 extract holding vessel
Valve switch；F4 is media outlet valve switch；F5 is recoil valve switch；F6 is elution waste liquid discharge valve switch；F7
Switched for eluent discharge valve；F8 is that eluent is discharged into the valve switch of collecting tank 1；F9 is that eluent is discharged into the valve of collecting tank 2
Switch；F10 is that eluent is discharged into the valve switch of collecting tank 3；
As shown in figs. 1 and 3, the multichannel pack chromatogram column system central control system can be carried out to valve F1, F2 and F3
Control, so as to be operated to whole separation process；
As shown in figs. 1 and 3, can after the multichannel pack chromatogram column system central control system is tested and analyzed by detector
To be controlled to valve F8, F9 and F10, different elution bioactive substances are classified so as to realize；
Required temperature when detector means are run：18 ~ 26℃；
Pressure when detector means are run is 1MPa ~ 8MPa；
The multichannel pack tomographic system compression pump 4 is simultaneously for extract tank 1, eluting solvent holding vessel 2 and elution media
Material in holding vessel 3 enters system, the relevant parameter of compression pump 4：Flow is the mL/min of 0.1 mL/min ~ 200；Work
Voltage is 220V, 50Hz；Power consumption is the kW of 1.5 kW ~ 3.0.
Embodiment 2
As shown in figure 1, opening valve F1, compression pump 4 is opened, it is ensured that other valves are closed so that the multichannel pack chromatogram
Ten chromatographic columns in column system are in paralleling model, and the medium that isolates and purifies in elution media tank 3 is shifted by compression pump
Into feed storage tank 6, then and then by Action of Gravity Field fill into each chromatographic column, it is ensured that medium is tight in the chromatography column
It is real；
Valve F3 is opened, compression pump 4 is opened, it is ensured that other valves are closed, and the material in 1 extract tank is shifted by compression pump
Into feed storage tank, then it is loaded in each chromatographic column；
Valve F2 and F7 are opened, compression pump 4 is opened, it is ensured that other valves are closed, and the eluting solvent in eluting solvent tank is passed through
Compression pump is transferred in feed storage tank, and then each chromatographic column is carried out to elute simultaneously；Detector to eluent by carrying out
Analysis detection, automatic classification collection is carried out to the biological substance that affords, by PLC central control system control valves F8,
F9 and F10 so that corresponding bioactive substance has respectively entered 1 ~ n of collecting tank；
After elution terminates, the medium outlet F4 on the recoil valve F5 and feed storage tank on elution discharge port system is opened, is protected
The closing of other valves is demonstrate,proved, can be by the elution media in all chromatographic columns by injecting mutually deserved cleaning solvent to recoil valve F5
Recoil comes out；And whole chromatogram column system is cleaned.
Claims (7)
1. multichannel pack tomographic system device of the present invention, it is characterised in that the system by central control system, carry
Take thing tank, eluting solvent tank, elution media tank, compression pump, multichannel pack chromatogram column system, detector and collecting tank etc. eight
Part is constituted.
2. multichannel pack tomographic system according to claim 1, it is characterised in that with multichannel pack chromatographic column system
System, pack chromatographic column and elution discharge that the multichannel pack chromatogram column system is made up of feed storage tank, ten root chromatogram columns
System is constituted.Feed storage tank is located at the upper end of whole pack chromatographic column, the top of feed storage tank have water inlet, charging aperture and
Wide-mouth form, its bottom has inside media outlet, feed storage tank and ten root chromatogram column UNICOMs.The height h1 of feed storage tank is
1.5 m；The height h2 of chromatographic column is 3m；The height h3 for eluting discharge system is 15cm;The height h4 and tank of collecting tank 1,2 and 3
Diameter d1 is 1.13m.
3. central control system according to claim 1, it is characterised in that central control system is by computer, PLC centers
Controller and detector composition.The instruction that PLC central controller is obtained according to detector is controlled to valve F1, F2, F3, from
And the operation required for being carried out to whole multichannel pack tomographic system.
4. central control system according to claim 1, it is characterised in that central control system is by computer, PLC centers
Controller and detector composition.The instruction that PLC central controller is obtained according to detector is controlled to valve F8, F9 and F10,
So as to classify to the bioactive substance afforded.
5. multichannel pack tomographic system according to claim 1, it is characterised in that in multichannel pack thin layer chromatography post
A variety of solid-phase medias can be filled, various bioactivators are isolated and purified so as to realize.
6. multichannel pack tomographic system according to claim 1, it is characterised in that multichannel pack tomographic system is washed
De- tank can be multiple, so as to realize that different solvents are isolated and purified to bioactive substance.
7. multichannel pack tomographic system according to claim 1, it is characterised in that the multichannel pack tomographic system
After being isolated and purified to bioactive substance, valve F4 and F5 can be opened, by F5 valves by closing valve F6 and F7
Injection cleaning solvent is cleaned to whole chromatogram column system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710471112.9A CN107132299A (en) | 2017-06-20 | 2017-06-20 | A kind of multichannel pack tomographic system |
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| CN201710471112.9A CN107132299A (en) | 2017-06-20 | 2017-06-20 | A kind of multichannel pack tomographic system |
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| CN107132299A true CN107132299A (en) | 2017-09-05 |
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| CN201710471112.9A Pending CN107132299A (en) | 2017-06-20 | 2017-06-20 | A kind of multichannel pack tomographic system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108495099A (en) * | 2018-06-05 | 2018-09-04 | 哈尔滨工业大学 | A kind of novel compositions annular arrangement chromatography video monitoring system |
| CN109954296A (en) * | 2018-12-12 | 2019-07-02 | 江苏哈工药机科技股份有限公司 | A kind of anthocyanidin isolates and purifies device and isolation and purification method |
| CN110201420A (en) * | 2019-06-27 | 2019-09-06 | 上海渔霁生物技术有限公司 | A kind of chromatographic system and method separating organic acid and organic acid esters |
| CN113769438A (en) * | 2021-10-15 | 2021-12-10 | 温州大学 | Large-caliber chromatographic stationary phase with double integral structures and in-situ preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108495099A (en) * | 2018-06-05 | 2018-09-04 | 哈尔滨工业大学 | A kind of novel compositions annular arrangement chromatography video monitoring system |
| CN109954296A (en) * | 2018-12-12 | 2019-07-02 | 江苏哈工药机科技股份有限公司 | A kind of anthocyanidin isolates and purifies device and isolation and purification method |
| CN110201420A (en) * | 2019-06-27 | 2019-09-06 | 上海渔霁生物技术有限公司 | A kind of chromatographic system and method separating organic acid and organic acid esters |
| CN113769438A (en) * | 2021-10-15 | 2021-12-10 | 温州大学 | Large-caliber chromatographic stationary phase with double integral structures and in-situ preparation method thereof |
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Application publication date: 20170905 |