CN205538588U - Unmarked tumor cells detection diagnosis device - Google Patents
Unmarked tumor cells detection diagnosis device Download PDFInfo
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- CN205538588U CN205538588U CN201521016028.0U CN201521016028U CN205538588U CN 205538588 U CN205538588 U CN 205538588U CN 201521016028 U CN201521016028 U CN 201521016028U CN 205538588 U CN205538588 U CN 205538588U
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The utility model discloses an unmarked tumor cells detection diagnosis device is equipped with laser lamp -house (1) and ultrasonic probe (5) laser lamp -house (1) and ultrasonic probe (5) between, be equipped with unicellular miniflow pipe (4). Adopt above -mentioned technical scheme, can detect tumor cells fast, quantitatively, can realize that early discovery detects tumor cells, it is significant to the cure rate that improves neoplastic disorder, realize that unmarked cell detects, the testing result is more accurate, and is convenient, for the treatment of tumor cells's transfer, recurring prognosis brought convenient, equipment structure is simple, make things convenient for the operation, wicresoft, security are high.
Description
Technical field
This utility model belongs to preclinical medicine scientific research and the technical field of clinical medicine checkout and diagnosis instrument and equipment.More specifically, this utility model relates to a kind of unmarked tumor cell checkout and diagnosis device.
Background technology
Tumor cell is a kind of skin carcinoma that mortality rate is the highest, accounts for about 2/3rds of all skin carcinoma Died Patients, and it has the advantages that grade malignancy is high, transfer is very fast, hazardness is big, and often before doctor makes diagnosis, cancerous cell has spread to systemic.In recent decades, the M & M of whole world tumor cell increases rapidly with the speed of annual 3%-5%,
Though China belongs to tumor cell Di Fa district, but sickness rate is up to every 10 years increase twice in recent years, and material life and cultural life to people cause the biggest burden.
Malignant cell, by exception melanocyte hyperplasia initiation, has easily transfer, grows the features such as rapid, grade malignancy height and prognosis mala, be one the most fatal in skin carcinoma, account for the extreme portions of cutaneous tumor death.Malignant cell is a kind of common and the most ever-increasing skin cancer of sickness rate, according to statistics, only in the U.S., the most just has 25000 malignant melanoma new cases, death about 6000 people.
Though China belongs to the Di Fa district of tumor cell, but sickness rate increases year by year with 3%-8% in recent years.Tumor cell is dangerous big, and its early diagnosis is significant to its cure rate.
Research shows: if in early days discovery timely to tumor cell, treating in time, survival is up to 60%-80%.When tumor cell by early diagnosis and by surgical excision after, the probability that patient is survived 5 years is up to 95%.Therefore, the early stage detection of tumor cell seems extremely important.
At present, tumor cell is diagnosed the micro-judgment relying on doctor more, the method that such as national cancer institute proposes " ABCD " early diagnosis:
A represents " asymmetry (unsymmetry) ", and common nevus is rounded or oval, breaks it into two, two hemihedrism；Malignant cell is often irregular, and two halves are asymmetric；
B represents " border (edge) ", and common nevus regular edges is smooth completely, clear with surrounding skin boundary, and malignant cell edge is uneven, many indentations；
C represents " color (color) ", and common nevus is brown color, brown or black, and malignant cell often mixes the multiple colors such as redness, white, blueness on the basis of brown color or sepia；
D represents " diameter (diameter) ", common nevus many ＞ 5mm, malignant cell normal ＜ 5mm.
Owing to current diagnostic means relies solely on the experience of doctor, it is impossible to realizing detection by quantitative in early days, the most late when patient typically goes to a doctor, therapeutic effect is not satisfied at all.How to realize tumor cell detection by quantitative in early days and become a problem demanding prompt solution.
Utility model content
This utility model provides a kind of unmarked tumor cell checkout and diagnosis device, its objective is to realize tumor cell early stage, quick, the most accurately detection, separates tumor cell simultaneously, captures.
To achieve these goals, the technical scheme that this utility model is taked is:
Unmarked tumor cell checkout and diagnosis device of the present utility model, is provided with LASER Light Source and ultrasonic probe, between described LASER Light Source and ultrasonic probe, is provided with unicellular microflow channels.
Cell separation, capture micro-fluidic chip it is provided with in the port of described unicellular microflow channels.
Micro structure is etched on described cell separation, capture micro-fluidic chip；Or the integrated microcavity volume array with filtering function on described cell separation, capture micro-fluidic chip；Or on described cell separation, capture micro-fluidic chip, magnetic field is set.
The laser that described LASER Light Source sends passes through laser post lens and diaphragm, and hot spot focuses on a bit in unicellular microflow channels.
The laser instrument of described LASER Light Source, its wavelength is 1064nm；Or it is 808nm；Or it is femtosecond laser.
The type of described laser instrument is the one in solid state laser, semiconductor laser, gas laser, optical fiber laser and dye laser.
The holding wire of described ultrasonic probe is connected with computer data processing system.
Described ultrasonic probe is the one in monocrystalline compressional wave normal probe, twin crystal compressional wave normal probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface wave probe.
The frequency range of described ultrasonic probe is 1-100MHz.
In order to realize the goal of the invention identical with technique scheme, this utility model additionally provides the checkout and diagnosis method being applied to above-described unmarked tumor cell checkout and diagnosis device, and its technical scheme is:
The laser that described LASER Light Source sends is focused in unicellular microflow channels by hot spot after laser post lens and diaphragm；When the tumor cell in blood flow passes through laser focus point (form) in this unicellular microflow channels, the tumor cell of process is irradiated with a laser to expand rapidly and sends ultrasonic signal；
The ultrasonic signal that tumor cell sends is detected by ultrasonic probe；Described ultrasonic probe is transferred to computer data processing system after ultrasonic signal being detected；
The ultrasonic signal of the computer data processing system tumor cell to detecting processes and at the cell number of the focus point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit interval (the most per minute)；Computer data processing system, by being effectively retained the frequency band signals that tumor cell produces, filters the noise signal of other wave band；Finally testing result is shown over the display.
This utility model uses technique scheme to provide the benefit that:
1, can rapidly and quantitatively detect tumor cell, early discovery detection tumor cell can be realized, significant to the cure rate improving tumor disease；
2, realizing n cell detection, eliminate cell dyeing, a series of chemical treating processes such as fixing, remain the primitive character of cell, testing result is more accurate, convenient；
3, a new detection means is provided for Nasopharyngeal neoplasms research and preclinical applications；
4, the quantity statistics for tumor cell is provided convenience, and transfer, the treatment of recurrence prognosis for tumor cell bring conveniently；
5, this device structure operation simple, convenient, Wicresoft, safety height；
6, the micro-fluidic chip in equipment can realize the separation of cell, captures the research such as species analysis, drug screening based on cellular level outside the cultivation to cell, activity assay, intracellular and is with a wide range of applications；
7, use liquid crystal display, there is the advantages such as display quality interface high, digital, volume are little, lightweight and low in energy consumption.
Accompanying drawing explanation
Labelling in accompanying drawing content and figure is briefly described as follows:
Fig. 1 is the schematic diagram of unmarked tumor cell checkout and diagnosis device of the present utility model.
Reference:
1, LASER Light Source, 2, post lens, 3, diaphragm, 4, unicellular microflow channels, 5, ultrasonic probe, 6, computer data processing system, 7, cell separation, capture micro-fluidic chip.
Detailed description of the invention
Below against accompanying drawing, by the description to embodiment, detailed description of the invention of the present utility model is described in further detail, to help those skilled in the art that inventive concept of the present utility model, technical scheme are had more complete, accurate and deep understanding.
Structure of the present utility model as shown in Figure 1, for a kind of unmarked tumor cell checkout and diagnosis device.This utility model further relates to tumor cell checkout and diagnosis method, a kind of can in early days detection by quantitative and the method and apparatus of diagnosing tumour cell, the method can realize the early stage of tumor cell and quickly detect, and realizing cell separation, capture, research and clinical treatment to Nasopharyngeal neoplasms simultaneously has great importance and application prospect.
In order to solve the problem of prior art existence and overcome its defect, it is achieved the goal of the invention that tumor cell in early days, quickly, the most accurately detects, the technical scheme that this utility model is taked is:
As it is shown in figure 1, unmarked tumor cell checkout and diagnosis device of the present utility model, it is provided with LASER Light Source 1 and ultrasonic probe 5, between described LASER Light Source 1 and ultrasonic probe 5, is provided with unicellular microflow channels 4.
Port in unicellular microflow channels goes out to be provided with cell separation, capture micro-fluidic chip 7.
Micro structure is etched on described cell separation, capture micro-fluidic chip 7；Or the integrated microcavity volume array with filtering function on described cell separation, capture micro-fluidic chip 7；Or on described cell separation, capture micro-fluidic chip 7, magnetic field is set.
The laser that described LASER Light Source 1 sends passes through laser post lens 2 and diaphragm 3, and hot spot focuses on a bit in unicellular microflow channels 4.
These post lens can be that single post lens can also form for battery of lens, and diaphragm is adjustable diaphragm.The laser that LASER Light Source sends is by being stripe shape or other shapes of hot spot by spot shaping after laser post lens and diaphragm.
When the tumor cell in blood flow passes through laser focus point (form) in this unicellular microflow channels, the tumor cell of process is irradiated with a laser to expand rapidly and sends ultrasonic signal.This unicellular microflow channels can also be various cell microchannel.
Research shows, the photoacoustic signal of tumor cell differs from normal cell, has when specific wavelength laser irradiates and produces stronger ultrasonic signal higher than Normocellular absorbability.
Utilizing this principle, this utility model provides above-mentioned unmarked tumor cell checkout and diagnosis device.This device passes through cylindrical mirror, diaphragm, and by the laser focusing that sends from LASER Light Source to unicellular microflow channels, this unicellular microflow channels only allows that blood flow is unicellular to be passed through.The ultrasonic probe 5 that the ultrasonic signal that tumor cell sends is placed near unicellular microflow channels detects, the ultrasonic signal detected is delivered in computer data processing system 6 by ultrasonic probe 5, the signal detected is processed and at the cell number of the focus point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit interval (the most per minute) by computer data processing system 6, and display is over the display.
Described LASER Light Source 1 can comprise the laser instrument of multi-wavelength, and the wavelength of laser instrument can be the different wavelength such as 1064nm, 808nm, femtosecond laser.
The type of described laser instrument can be the types such as solid state laser, semiconductor laser, gas laser, optical fiber laser and dye laser.
The holding wire of described ultrasonic probe 5 is connected with computer data processing system 6.
Described ultrasonic probe 5 can be the model such as monocrystalline compressional wave normal probe twin crystal compressional wave normal probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface wave probe probe.
The frequency range of described ultrasonic probe 5 is 1-100MHz, and other frequency range.
In order to realize the goal of the invention identical with technique scheme, this utility model additionally provides the checkout and diagnosis method being applied to above-described unmarked tumor cell checkout and diagnosis device, and its technical scheme is:
In the laser that described LASER Light Source 1 sends is by after laser post lens 2 and diaphragm 3, hot spot focuses on unicellular microflow channels 4；When the tumor cell in blood flow passes through laser focus point (form) in this unicellular microflow channels 4, the tumor cell of process is irradiated with a laser to expand rapidly and sends ultrasonic signal；
The ultrasonic signal that tumor cell sends is detected by ultrasonic probe 5；Described ultrasonic probe 5 is transferred to computer data processing system 6 after ultrasonic signal being detected；
The ultrasonic signal of the computer data processing system 6 tumor cell to detecting processes and at the cell number of the focus point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit interval (the most per minute)；Computer data processing system 6, by being effectively retained the frequency band signals that tumor cell produces, filters the noise signal of other wave band；Finally testing result is shown over the display.
This computer data processing system is all ultrasonic signal processing systems.
Application example of the present utility model:
Being mounted with that 1064nm laser instrument, this laser instrument are pulse laser within the system, the single pulse energy of laser instrument is 20uj；Laser instrument is solid state laser, and the light beam of laser instrument passes through post lens focus, and post lens are biconvex lens, and focal length is 300mm；Grating is tunable gratings, finally modulation to 1mm；Laser facula after over-focusing focuses on the centre of unicellular microflow channels, and unicellular microflow channels uses unicellular sheath fluid pipe, and computer data processing system uses the counting procedure utilizing MATLAB software programming.
Utilizing said apparatus, this utility model to achieve the detection by quantitative of tumor cell in blood flow in sheath fluid pipe, cell quantification analysis of accounts, experiment has obtained good effect, has reached target.
Above in conjunction with accompanying drawing, this utility model is exemplarily described; obviously this utility model implements and is not subject to the restrictions described above; as long as have employed the improvement of the various unsubstantialities that method of the present utility model design is carried out with technical scheme; or the most improved design of the present utility model and technical scheme are directly applied to other occasion, all within protection domain of the present utility model.
Claims (5)
1. a unmarked tumor cell checkout and diagnosis device, it is characterised in that:
Described checkout and diagnosis device is provided with LASER Light Source (1) and ultrasonic probe (5), between described LASER Light Source (1) and ultrasonic probe (5), is provided with unicellular microflow channels (4)；
Cell separation, capture micro-fluidic chip (7) it is provided with in the port of described unicellular microflow channels (4)；Micro structure is etched in described cell separation, capture micro-fluidic chip (7)；Or the integrated microcavity volume array with filtering function is gone up in described cell separation, capture micro-fluidic chip (7)；Or in described cell separation, capture micro-fluidic chip (7), magnetic field is set；
The holding wire of described ultrasonic probe (5) is connected with computer data processing system (6).
2. according to the unmarked tumor cell checkout and diagnosis device described in claim 1, it is characterised in that: the laser that described LASER Light Source (1) sends passes through laser post lens (2) and diaphragm (3), and hot spot focuses on a bit in unicellular microflow channels (4).
3. according to the unmarked tumor cell checkout and diagnosis device described in claim 2, it is characterised in that: the laser instrument of described LASER Light Source (1), its wavelength is 1064nm；Or it is 808nm；Or it is femtosecond laser.
4. according to the unmarked tumor cell checkout and diagnosis device described in claim 3, it is characterised in that: the type of described laser instrument is the one in solid state laser, semiconductor laser, gas laser, optical fiber laser and dye laser.
5. according to the unmarked tumor cell checkout and diagnosis device described in claim 1, it is characterised in that: described ultrasonic probe (5) is the one in monocrystalline compressional wave normal probe, twin crystal compressional wave normal probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface wave probe.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510629287 | 2015-09-28 | ||
| CN2015106292879 | 2015-09-28 |
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| CN205538588U true CN205538588U (en) | 2016-08-31 |
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| CN201510906209.9A Pending CN105352875A (en) | 2015-09-28 | 2015-12-07 | Unmarked tumor cell detecting and diagnosing device and detecting and diagnosing method thereof |
| CN201521016028.0U Active CN205538588U (en) | 2015-09-28 | 2015-12-07 | Unmarked tumor cells detection diagnosis device |
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| CN201510906209.9A Pending CN105352875A (en) | 2015-09-28 | 2015-12-07 | Unmarked tumor cell detecting and diagnosing device and detecting and diagnosing method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106510727A (en) * | 2016-12-27 | 2017-03-22 | 上海交通大学 | Early-stage mark-free clinic real-time detection device for melanoma |
| CN110412123A (en) * | 2019-07-23 | 2019-11-05 | 南方科技大学 | A kind of micro-fluidic imaging system of optoacoustic and method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108444896A (en) * | 2018-01-10 | 2018-08-24 | 深圳先进技术研究院 | A kind of optoacoustic detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20050070803A1 (en) * | 2003-09-30 | 2005-03-31 | Cullum Brian M. | Multiphoton photoacoustic spectroscopy system and method |
| US20090156932A1 (en) * | 2007-12-13 | 2009-06-18 | Board Of Trustees Of The University Of Arkansas | Device and method for in vivo flow cytometry using the detection of photoacoustic waves |
| CN203530312U (en) * | 2013-07-02 | 2014-04-09 | 周辉 | Detection diagnosis device for tumor cells or other pathological cells |
| CN104483482A (en) * | 2014-12-22 | 2015-04-01 | 常熟市创新模具有限公司 | Method for manufacturing device used for detecting tumor cells |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106510727A (en) * | 2016-12-27 | 2017-03-22 | 上海交通大学 | Early-stage mark-free clinic real-time detection device for melanoma |
| CN106510727B (en) * | 2016-12-27 | 2024-04-02 | 上海交通大学 | Be used for early no mark clinical real-time detection device of melanoma |
| CN110412123A (en) * | 2019-07-23 | 2019-11-05 | 南方科技大学 | A kind of micro-fluidic imaging system of optoacoustic and method |
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| CN105352875A (en) | 2016-02-24 |
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| C14 | Grant of patent or utility model | ||
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Effective date of registration: 20171010 Address after: 510700, Guangzhou City, Guangdong province Whampoa District hi tech Industrial Development Zone Science City open source Avenue No. 11 B10 building 3 floor 306 room Patentee after: Guangzhou Rui Rui Biological Technology Co., Ltd. Address before: 241000 Software Park in Anhui province Wuhu City Economic and Technological Development Zone Branch Center Room 0123 Patentee before: Zhou Hui |
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| TR01 | Transfer of patent right |