CN204832194U

CN204832194U - Biological sample testing cassette

Info

Publication number: CN204832194U
Application number: CN201520490274.3U
Authority: CN
Inventors: 刘圣强; 龚挺锋; 何焕军
Original assignee: Acon Biotech Hangzhou Co Ltd
Current assignee: Acon Biotech Hangzhou Co Ltd
Priority date: 2015-07-03
Filing date: 2015-07-03
Publication date: 2015-12-02
Anticipated expiration: 2025-07-03

Abstract

The utility model relates to a biological sample test technical field especially relates to a biological sample testing cassette, be equipped with in the testing cassette one relatively testing cassette motion the push rod and with push rod sliding fit's spout, at least one of push rod is equipped with at least one spacing arch on the side, be equipped with on the spout with the spacing recess of spacing protruding block. The utility model discloses set up the push rod under initial condition, spacing recess and spacing protruding block can make more stable the installing in biological sample testing cassette's inside of push rod, the release in advance of reagent when having avoided not bumping.

Description

Biological test sample box

Technical field

The utility model relates to detection of biological samples technical field, particularly relates to a kind of biological test sample box.

Background technology

Medical science in-vitro diagnosis plays the part of the role quite reused in medical industry now, its can be qualitative or quantitative measurement human fluid in the change of various Biological indicators, to provide the consulting such as medical diagnosis on disease or treatment index, such as, the detection of the glycosylated hemoglobin (HbA1c) in blood for diabetes diagnosis and control most important.

Glycosylated hemoglobin is the product that in blood of human body, endoerythrocytic haemoglobin is combined with blood sugar, and when the concentration of glucose in blood is higher, the content of the HbA1c that human body is formed also can be relatively high.In human body, erythrocytic mean lifetime is 120 days, before cell death, in blood, the content of HbA1c also can keep relatively constant, therefore, glycosylated hemoglobin test can reflect patient's glycemic control situation of nearly 8 ~ 12 weeks usually, not by the impact of a blood sugar rising or reduction once in a while.

Also the multiple concentration being designed for detect analytes is had in prior art, as:

As shown in Figure 1, US1562237 discloses a kind of biological test sample box, biological test sample box comprises reaction channel and liquid reagent storage part, reaction channel is provided with dry reagent, liquid reagent storage part is used for depositing damping fluid or other liquid reagent, liquid reagent storage part comprises storage body 30', store body to be sealed by seal or film 32', film 32' has a far-end 33', distally directly tearing film makes film be separated with storage body, is discharged in reaction channel by the reagent stored in body.

When releasing liquid reagent, need artificially to separate being originally sealed in the film 32' stored on body 30', then film is removed.Although aforesaid way can tear film, releasing liquid reagent, but, the far-end 33' extended by one section tears film very not easily from storage body 30', as people for tearing time overexertion or firmly kick the beam easily cause film to disconnect or tear off not exclusively, liquid reagent can not be discharged completely, and during detection, liquid reagent is very few, causes the deviation of testing result; On the other hand, in the non-detection period, film 32' is exposed aerial, easily occurs the risk that artificial or other factors is ripped or destroyed.

As shown in Figure 2, US5272093 discloses one " reagent container and delivering method thereof ", comprise reagent storage chamber 12' and be sealed in the seal 40' on reagent storage chamber, seal is folding two-layer setting, one deck is used for sealed reagent and stores chamber, another layer extends to outside, reagent storage chamber and forms extension 42', extension 42' for tearing the reagent in release reagent storage chamber.When releasing liquid reagent, need artificially the script seal 40' be sealed on the 12' of reagent storage chamber to be separated, if overexertion or firmly kick the beam and as easy as rolling off a logly cause film to disconnect or tear off not exclusively, liquid reagent is caused not discharge completely, when making detection, liquid reagent is very few, causes the deviation of testing result.

As shown in Figure 3, US8846380 discloses one " for detecting the biological test sample box of glycosylated hemoglobin concentration ", biological test sample box comprises the firstth district for holding kit blood sample, for holding the secondth district of wash solution, detection zone and pack, reagent in pack and wash solution separately store, and seal up for safekeeping with aluminium foil 120'.When pack is inserted in biological test sample box, aluminium foil is torn by biological test sample box, on the first reaction zone that reagent in pack and wash solution are temporarily stored in biological test sample box respectively and second reaction zone, reacted with blood sample successively by the rotation of biological test sample box, thus solve storage and the assignment problem of reagent.

Above-mentioned biological test sample box, when detecting, first, needs the release portion 130' on artificial folding aluminium foil, release portion 130' is made to aim at the clamper detected in box, again the pack after aiming at is inserted and detect box, make to detect box and can excise release portion, realize being separated of aluminium foil and pack.This design relative complex, operation steps is various, and, need artificial aligning when grafting, easily occur the situation that grafting is not in place and cause grafting repeatedly.On the other hand, biological test sample box and pack adopt split-type design, and the opened type design of biological test sample box, if misoperation, very easily falls into foreign matter in biological test sample box, impact the accuracy of testing result.Moreover in the non-detection period, release portion 130' is exposed aerial, easily there is the risk that artificial or other factors is ripped or destroyed.

Summary of the invention

Technical problem to be solved in the utility model is for the above-mentioned problems in the prior art, provides a kind of biological test sample box.

For solving the problem, the technical solution of the utility model is:

A kind of biological test sample box, being provided with one in described testing cassete can the push rod of testing cassete motion and the chute that is slidably matched with push rod relatively, at least one side of described push rod is provided with at least one spacing preiection, and chute is provided with the limited impression engaged with described spacing preiection.

Preferably, described spacing preiection is two, is separately positioned on the left and right side of push rod.

Preferably, described spacing preiection is arranged on the top of push rod side.

Preferably, the described push rod place of keeping to the side is provided with the cell body of at least one hollow out, and described spacing preiection is arranged on the lateral wall of cell body, and spacing preiection and cell body are arranged in pairs.

Preferably, described push rod is provided with a groove.

Preferably, described push rod is all housed in testing cassete.

Preferably, described chute is provided with division board, the inner space of division board isolation chute and testing cassete.

Be compared to prior art, the beneficial effects of the utility model are:

1. biological test sample box of the present utility model is an independent entirety, it is inner that reagent storage portion and push rod are all encapsulated in biological test sample box, when reacting, only need and detect box and coordinate, single stepping, be inserted in external unit by biological test sample box, the quick release of reagent in reagent storage portion can be realized.Said structure by the people in detection reaction for manual operation section tapers to the degree simplified most, improve the automaticity of reaction, when reacting, artificially manual operation steps only comprises sampling rod collected specimens and added to by sample in biological test sample box inserts the step of external unit with biological test sample box.

2. it is inner that the various piece that biological test sample box of the present utility model comprises all is housed in biological test sample box, especially reagent storage portion and push rod are included in biological test sample box inside, make artificially manually all can not touch reagent storage portion and push rod, when only stretching in biological test sample box by the external thrust detecting box in external unit, just can impel push rod action; When having stopped unreacted, because manual operation is improper or other factors, seal part and reagent storage portion are torn off, causes the possibility that reagent spills in advance.

3. the opening on biological test sample box of the present utility model be all that gap coordinates between each parts, provide the reaction compartment that biological test sample box is inner simple, ensure that the purity of each reaction reagent, avoid foreign and be mixed into possibility in biological test sample box, improve the precision of reaction.

4. reagent storing device of the present utility model is individual components, is encapsulated in biological test sample box, and reagent storing device comprises the reagent container cavity for depositing solid particle reagent or powder particle reagent.Solid particle reagent or powder particle reagent are sealed in reagent storing device, avoid the random movement of reagent in biological test sample box, avoid the problem needing in prior art to be fixed dried reagent, simultaneously, under sealing state, also improve the term of validity storage life under this reagent normal temperature, itself and other reagent difference is come, under particularly suitable normal temperature, compares the reagent being difficult to ensure and depositing.

5. reagent storage portion of the present utility model is provided with latex freeze-drying ball, achieves on the one hand the maximization to the protection of latex antibody reactivity, on the other hand, improves term of validity storage life under reagent normal temperature greatly.

6. biological test sample box of the present utility model is by configuring number and the arrangement thereof of reagent container cavity in reagent storage portion, with coordinating of push rod automation function, can realize that multiple reagent discharges simultaneously, multiple reagent adds successively or simultaneously according to reaction time and order and coordinate oven dry reagent, complete the possibility of various sample detection.

7. detection system of the present utility model is when biological test sample box inserts, push rod action can be promoted rapidly, simultaneously, by biological test sample box fast firm being fixed on detect in box, ensure that light hole is aimed in detection zone, i.e. single stepping, complete simultaneously reagent release, biological test sample box fix and detection zone to multiple functions such as light, simplify reactions steps most.

8. biological test sample box of the present utility model arranges push rod in an initial condition, and limited impression engages with spacing preiection, can make the inside being arranged on biological test sample box that push rod is more stable, avoids the release in advance of reagent when not touching.

Accompanying drawing explanation

Fig. 1 is the structural representation of biological test sample box in prior art.

Fig. 2 is the structural representation of reagent container in prior art.

Fig. 3 is the partial structurtes schematic diagram of biological test sample box in prior art.

Fig. 4 is that the axle of partial structurtes in the utility model detection system measures intention.

Fig. 5 be in the utility model biological test sample box panel hide after structural representation.

Fig. 6 is the structural representation after the utility model biological test sample box dorsulum is hidden.

Fig. 7 is the syndeton schematic diagram of reagent storage part in the utility model, seal and reagent release portion.

Fig. 7 A be in Fig. 7 seal tear after structural representation.

Fig. 8 is the structural representation of reagent storage part in the utility model.

Fig. 8 A is the structural representation of a reagent storage part angle in the utility model.

Fig. 8 B is the structural representation of another angle of reagent storage part in the utility model.

Fig. 9 is the structural representation of a kind of embodiment of reagent storage part in the utility model.

Figure 10 is the structural representation of the another kind of embodiment of reagent storage part in the utility model.

Figure 11 is the syndeton schematic diagram of upper cover in the utility model biological test sample box, reagent storage portion and reagent release portion.

Figure 12 is the syndeton schematic diagram of box body and push rod in the utility model biological test sample box.

Figure 12 A is the structural representation in the utility model biological test sample box after the relative box body slip of push rod.

Figure 13 is the structural representation that in the utility model biological test sample box, box body is connected another kind of embodiment with push rod.

Figure 14 is the axle geodesic structure schematic diagram of release portion in the utility model biological test sample box.

Figure 15 is the structure for amplifying schematic diagram at A place in Fig. 6.

Figure 16 is the axle geodesic structure schematic diagram that in the utility model biological test sample box, box body is connected with push rod.

Figure 16 A is the structure for amplifying schematic diagram at C place in Figure 16.

Figure 17 is the axle geodesic structure schematic diagram of the another kind of embodiment that in the utility model biological test sample box, box body is connected with push rod.

Figure 18 is the structure for amplifying schematic diagram at D place in Fig. 5.

Figure 19 is that the axle of the utility model biological test sample box external structure measures intention.

Figure 19 A is the backsight structural representation of the utility model biological test sample box.

Figure 20 is the structural representation of upper cover in the utility model biological test sample box.

Figure 20 A is the structure for amplifying schematic diagram at B place in Figure 20.

Figure 20 B is another angled arrangement schematic diagram of upper cover in the utility model biological test sample box.

Figure 21 is the syndeton schematic diagram in upper cover and reagent storage portion in the utility model biological test sample box.

Figure 22 is the explosive view of the utility model biological test sample box.

Figure 23 is the cross-sectional view that the utility model detects box.

Figure 24 is the structural representation that the utility model detects internal part in box.

Figure 25 is that the utility model biological test sample box inserts and to detect in box push rod by the cross-sectional view of push rod jack-up.

Figure 26 is the structural representation that the utility model detects box Elastic part.

Figure 27 is the structural representation that the utility model detects shell fragment in box.

Figure 28 is in the reagent view of step 0 when being the detection of the utility model biological test sample box.

Figure 28 A-28F is in the reagent view of step 1-6 when being the detection of the utility model biological test sample box respectively.

Embodiment

Further describe the utility model below in conjunction with drawings and Examples, but protection domain of the present utility model is not limited to this.

As shown in Figure 4, detection system of the present utility model is used for the concentration detecting analyte in biological sample fast, comprise biological test sample box 100 and external unit, external unit is the equipment except biological test sample box 100 body, wherein, external unit is also referred to as checkout equipment, and external unit or checkout equipment are provided with a detection box 200, and biological test sample box 100 and detection box 200 support the use.Biological test sample box 100, as the container of reaction, can realize the storage of plurality of reagents, the order of plurality of reagents adds and the function such as devil liquor recovery.Detect box 200 for placing biological test sample box 100, detecting box 200 is arranged on checkout equipment, and provide that biological test sample box 100 rotates by checkout equipment, the machine power of reagent mixing, reagent release etc. in biological test sample box 100 and control, external unit is also provided with optical device, optical device is used for the concentration detecting thing in tracer liquid sample simultaneously.

As shown in Figure 5 and Figure 6, reagent storage portion 102, reagent release portion 150, reacting part and detection zone 28 is provided with in biological test sample box 100.Reagent storage portion 102 and reagent release portion 150 are all encapsulated in biological test sample box 100.Preferably, be provided with a cavity in described biological test sample box 100, described reagent storage portion 102 is housed in cavity.Reagent storage portion 120 is encapsulated in biological test sample box 100 for individual components, is conducive to the rapid-assembling of biological test sample box.Reagent storage portion 102 is for sealed type storage solid particle, powder or liquid reagent, and reagent release portion 150 can open reagent storage portion 102 release reagent fast in the reacting part of biological test sample box 100 detect the cooperation of box 200 in checkout equipment under.Reacting part be used for biological sample and reagent temporary, mix and react, and by detection zone 28, the intermediate product reacted or final product to be detected.

As shown in Figure 7 and Figure 8, reagent storage portion 102 comprises at least one reagent container cavity, and reagent storage portion 102 is sealed by seal 104, and namely reagent container cavity is sealed by seal 104.Reagent storage portion 102 is also referred to as reagent storing device, and reagent storage portion 102 comprises multiple reagent container cavity, and multiple reagent container cavity becomes array distribution.The reagent type of quantity required for detection reaction of reagent container cavity, the order of interpolation set, such as one, two or multiple, reagent container cavity is independently each other, namely multiple reagent container cavity is provided with spacing each other, becomes array distribution.Such as, two reagent container cavities can be horizontal in a row shapes, also can be longitudinally become column-shaped, can also be diagonal angle distributions; For another example, multiple reagent container cavity can interval plug hole distribution.The capacity of reagent container cavity is also that the dosage required for detection reaction sets.

Preferred as one, described reagent storage portion comprises at least two row reagent container cavities, and often row comprise at least one reagent container cavity.Preferably, as shown in Figure 8, reagent storage portion 102 comprises two reagent container cavities, and two reagent container cavity left and right are transversely arranged.Namely reagent container cavity 106 and reagent container cavity 108 are in a row two row left and right distribution, and the bottom of reagent container cavity roughly in the same horizontal line.Therefore, when tearing seal 104, the reagent in reagent container cavity 106 and reagent container cavity 108 can together be discharged in biological test sample box 100.

As shown in Figure 9, as a kind of embodiment in reagent storage portion 102, the situation of two reagent container cavity longitudinal arrangements, namely reagent container cavity 110 and reagent container cavity 112 distribute up and down in two row one row, when partially torn seal 104, be positioned at the preferential release reagent of reagent container cavity 112 of lower row, according to the requirement in reaction time, continue to tear seal 104, make reagent container cavity 110 release reagent being positioned at row.Under the degree that control seal 104 is torn, just can add reagent successively, control the carrying out of reaction.

As shown in Figure 10, as the another kind of embodiment in reagent storage portion, four reagent container cavities are the situation of arrayed, and namely reagent container cavity 114, reagent container cavity 116, reagent container cavity 118 and reagent container cavity 120 is in two row four column-shaped distributions.Controlling under seal 104 degree of tearing, the reagent in the reagent container cavity 118 of lower row and reagent container cavity 120 preferentially discharges, the then reagent of the upper row of release.

Preferred as one, described reagent storing device comprises the reagent container cavity for depositing solid particle reagent or powder particle reagent.Solid particle reagent or powder particle reagent are sealed in reagent storing device, avoid the random movement of reagent in biological test sample box, avoid the problem needing in prior art to be fixed dried reagent, simultaneously, under sealing state, also improve the term of validity storage life under this reagent normal temperature, itself and other reagent difference is come, under particularly suitable normal temperature, compares the reagent being difficult to ensure and depositing.

Preferably, described solid particle reagent or powder particle reagent are lyophilized solid particle reagents or freeze-dried powder particle reagents.Preferably, described solid particle reagent is latex freeze-drying ball.Latex freeze-drying ball, achieves on the one hand the maximization to the protection of latex antibody reactivity, on the other hand, improves term of validity storage life under reagent normal temperature greatly.

Preferred as one, described reagent storing device also comprises the reagent container cavity for depositing liquid reagent.Reagent container cavity may be used for depositing solid particle, powder particle or liquid reagent, can set according to detection needs.

Preferred as one, as shown in Figure 8 A, at least one reagent container cavity is provided with injection orifice 130, and injection orifice 130 is communicated with reagent container cavity and space outerpace, and reagent container cavity and injection orifice 130 seal by seal 104.Preferably, injection orifice 130 is located at the reagent container cavity for depositing powder reagent or liquid reagent.In order to more easy seal fluid reagent or powder reagent, when sealing, first use seal 104 sealed reagent container cavity and by outside exposed for injection orifice 130, again liquid or powder reagent are injected in reagent container cavity, then sealing injection hole 130 again, ensure that the effect of sealing with this, and reduce the difficulty of seal fluid or powder reagent.Reagent container cavity and injection orifice by the sealing of same seal timesharing substep, can be sealed by two seals, as used sealing plug sealing injection hole etc. respectively.

Preferred as one, as shown in Figure 8 B, the back in reagent storage portion 102 is also provided with the cavity 132 for installing drying agent.When reagent storage portion 102 is arranged in biological test sample box 100, by the cooperation of cavity 132 and panel 10 inwall, drying agent is arranged in cavity 132.

Preferred as one, reagent storage portion 102 comprises two reagent container cavities, and two reagent container cavities are respectively used to store solid particle reagent and liquid reagent.The present embodiment comprises two reagent container cavities 106 with reagent storage portion 102,108 are described in detail for example, reagent container cavity 106 stores solid particle reagent and reagent container cavity 108 storage of liquids reagent, and is provided with injection orifice 130 in the upper end of reagent container cavity 108.First solid particle reagent is placed in reagent container cavity 106 when sealing, with the cavity hatch of seal 104 sealed reagent container cavity 106 and reagent container cavity 108, liquid reagent is injected in reagent container cavity 108 again, then utilizes seal 104 sealing injection hole 130.

Preferred as one, the bottom design of reagent container cavity takes the form of inclined plane.When release reagent, facilitate reagent to flow out fast, decrease reagent remaining in container cavity as far as possible, ensure that the precision that reagent volumes controls.

As shown in Figure 7, reagent storage portion 102 is sealed by seal 104, be sealed in reagent container cavity by reagent, seal 104 is under the effect of external force, can tear off with reagent container cavity, seal 104 tear off can be by from left to right, the mode such as from lower to upper, tear off from lower to upper for seal herein and be described, as shown in Figure 7 A.Seal 104 can be the conventional seal used in the prior art such as aluminium foil, film.

As is illustrated by figs. 11 and 12, reagent release portion 150 comprises push rod 152, and push rod 152 is connected with seal 104, described push rod 152 for external unit cooperatively interact make seal and reagent storage part from.As shown in Figure 7 A, namely seal 104 is pasted or is fixed on push rod 152, when external device acts on push rod 152, push rod 152 coordinates with the inwall of biological test sample box 100 motion produced relative to reagent storage portion 102, while push rod 152 moves, band dynamic seal 104 relative reagent storage part 102 moves, i.e. the action that tears off of seal 104, makes the reagent in reagent container cavity be discharged in biological test sample box 100.The motion of push rod 152 relative reagent storage part 102 can for by turning left from left to right, by the right side, from lower to upper etc. mode realize.

Preferred as one, as shown in Figure 7, one end sealed reagent storage part 102 of seal 104, is pasted onto on push rod 152 after the other end is folding.

Preferred as one, described push rod 152 is provided with the forced section cooperatively interacted with external unit.The thrust that forced section provides for accepting external unit.

Preferred as one, it is inner that push rod 152 is all housed in biological test sample box 100, and the thrust piece of external unit needs the forced section extending into biological test sample box 100 inside or act on push rod 152 by other means.Push rod 152 is all housed in biological test sample box, and human contact, less than the push rod in biological test sample box, artificially free-handly can not impel push rod 152 action, only after by external tool, just can make push rod 152 action.Doing so avoids in the non-detection period, maloperation or other reason people tear or destroy the possibility that seal 104 causes reagent to leak in advance.

Preferred as one, as shown in figure 13, in biological test sample box 100, be provided with chute 38.On the one hand, push rod 152 is embedded in slideway by chute 38, and on the other hand, chute 38 and push rod 152 are slidably matched.As an embodiment, chute 38 is arranged on backboard 12, and chute 38 can be designed as and is only made up of backboard 12 and two trough rims, and now, push rod 152 dual-side partial receipt is in chute 38.

Preferred as one, as shown in Figure 12 and Figure 12 A, described chute 38 is provided with division board 36, and division board 36 isolates the inner space of chute 38 and biological test sample box 100.As a kind of embodiment, chute 38 is the column of at least one end opening, four sides of column are made up of part backboard 12, chute 38 dual-side and division board 36, that is, chute 38 is tightly connected division board 36, division board 36 separates the inner space of chute 38 and biological test sample box 100, and it is outer for designing requirement that division board 36 height can not flow to biological test sample box with reactant liquor.Chute 38 is designed to an independently region, push rod 152 or external thrust part and reagent is kept apart, can avoid the contact of push rod 152 or external thrust part and reaction reagent and take away portion of reagent.

Preferred as one, as shown in figure 14, at least one side of described push rod 152 is provided with at least one spacing preiection 154.The inside being arranged on biological test sample box 100 that spacing preiection 154 can make push rod 152 more stable.Preferably, the quantity of spacing preiection 154 is two, is separately positioned on the left and right side of push rod 152.Preferably, described spacing preiection 154 is arranged on the top of push rod 152 side.When push rod 152 is arranged in biological test sample box 100, spacing preiection 154 on push rod 152 is under the extruding of biological test sample box 100, push rod 152 is made to produce elastic deformation, add the friction force of push rod 152 and biological test sample box 100, what make that push rod 152 can be stable is arranged in biological test sample box 100, also increases resistance when push rod 152 moves.

Preferred as one, as shown in figure 14, described push rod 152 place of keeping to the side is provided with the cell body 156 of at least one hollow out, and described spacing preiection 154 is arranged on the lateral wall of cell body 156, and spacing preiection 154 and cell body 156 are arranged in pairs.As a specific embodiment, spacing preiection 154 is two, two spacing preiections 154 are separately positioned on the left and right side of push rod 152, correspond, the cell body 156 of hollow out is also two, to be separately positioned on the left of push rod and right side keeps to the side place, namely the lateral wall of limit, left side cell body 156 is the left surface of push rod 152, the lateral wall of right edge cell body 156 is the right flank of push rod 152, and that is, spacing preiection 154 is arranged on the lateral wall of cell body 156.The setting of hollow out cell body 156, makes more easily to produce deformation during push rod 152 action, avoids because rubbing excessive and to cause push rod 152 to slide dumb.

If push rod 152 is in the deformed state elasticity that just easily loss is certain for a long time, and makes fastening effect not good, for solving the problem, preferred as one, as shown in figure 15, biological test sample box 100 is provided with the limited impression 158 coordinated with spacing preiection 154.Preferably, chute 38 is provided with the limited impression 158 engaged with described spacing preiection 154, and the quantity of limited impression 158 is identical with spacing preiection 154.When push rod 152 is under original state and normality, limited impression 158 engages with spacing preiection 154, and now, deformation does not occur push rod 152; When the promotion lower slider of push rod 152 in external force, push rod 152 produces deformation at spacing preiection 154 place and leaves limited impression 158.

Preferred as one, as shown in figure 14, described push rod is provided with a groove 162.Groove 162 is arranged on the upper end of push rod 152, and groove 162, for pasting groove, is pasted groove and is used for adhesive seal part 104.Before non-adhesive seal part, the bottom land place plane of groove 162 is a little less than groove 162 notch place plane, and when after adhesive seal part 104, seal 104 fills and leads up groove 162, makes push rod plane concordant.When avoiding push rod 152 to promote, cause the possibility of stripping because seal 104 exceeds push rod 152 surface.

Preferred as one, the forced section of push rod 152 is bottom surface or the back of push rod, when forced section is the bottom of push rod, External Force Acting is in the bottom surface of push rod, push rod 152 is made to tear seal 104 from lower to upper, when forced section is the back of push rod, External Force Acting, in the back of push rod, impels push rod 152 to tear seal 104 from lower to upper equally.The forced section of push rod 152 can also be the upper bottom surface of push rod, left surface or right flank, during for upper bottom surface, can pull push rod from top; During for left surface, push rod can be promoted from left to right; During for right flank, promotion push rod of can turning left from the right side.

As another kind of embodiment, the forced section of push rod 152 is connected with extraneous thrust piece magnetic, and namely the forced section of push rod 152, extraneous thrust piece are the magnet assembly attracted each other, as iron block, magnet etc.Push rod 152 and extraneous thrust piece are isolated by biological test sample box 100, under magnetic action, drive push rod 152 action by extraneous thrust piece.

Preferred as one, biological test sample box 100 is provided with an opening, and forced section is in said opening exposed, and forced section is by opening accepts external thrust.Namely external thrust part can stretch in opening, contacts and impel its action with the push rod 152 in biological test sample box 100, and aperture position arranges and arranges relevant to the forced section of push rod 152.

As a kind of embodiment, as shown in figure 16, the forced section of push rod 152 is the bottom surface of push rod, and aforesaid opening 34 is arranged on the base plate 4 of biological test sample box 100.Preferably, as shown in Figure 16 A, opening 34 is provided with chamfering 40, and push rod 152 is all housed in chute 38 also has certain interval, to avoid collision during maloperation apart from opening 34.External thrust part is introduced by the chamfering 40 of opening 34 and is stretched into chute 38, contacts with push rod 152, and impels push rod 152 and external thrust part and chute 38 to be slidably matched.

As another kind of embodiment, as shown in figure 17, the forced section of push rod 152 is the back of push rod 152, and aforementioned opening 34 is arranged on the backboard 12 of biological test sample box 100.Preferably, the back of push rod 152 is provided with fin or recess 160, and fin or recess 160 are forced section, fin or recess 160 exposed in opening 34.External thrust part contacts with fin or recess 160, and impels push rod 152 and chute 38 to be slidably matched.

Described reacting part comprises at least one reaction zone, and reaction zone receives the reagent that reagent storage portion 102 discharges.Reacting part comprises multiple reaction zone, the quantity of reaction zone and position setting are correlated with according to the quantity of reagent container cavity in reagent storage portion 102 and reactions steps, two kinds of reagent as discharged simultaneously can be temporarily stored in a reaction zone, also can be temporarily stored in respectively two each independently in reaction zone; If dry reagent in addition in reaction zone, also can other reaction zone be set separately, be interconnected between each reaction zone, the mixing of reagent in each reaction zone can be realized by rotating biological sample test box.

As shown in Figure 5 and Figure 6, detection zone 28 is provided with in reacting part, detection zone 28 can be arranged on the circulation passage of any agent of biological test sample box 100, and detection zone 28 generally adopts transparent material, and the transmitted light that optical device is sent or scattered light enter biological test sample box 100.Be provided with conducting element between reagent storage portion 102 and reacting part, more specifically, conducting element is arranged between reagent container cavity and corresponding reaction zone, and conducting element makes the reagent in reagent container cavity be discharged into fast and accurately in reaction zone.

Preferred as one, as shown in Fig. 5 and Figure 18, reacting part comprises at least one reaction zone, wherein at least one reaction zone is the first reaction zone 26, described first reaction zone 26 is for temporary solid particle reagent, first reaction zone 26 comprises support portion and stop part, forms a gap 47 between support portion and stop part, and the breadth extreme in gap 47 is less than the minimum widith of solid particle reagent.Gap between support portion and stop part is for stopping that the solid particle reagent in the first reaction zone enters other reaction zone, and what make that solid particle reagent can be stable is temporarily stored on support portion.As a kind of embodiment, support portion is a step 46, and stop part is a baffle plate, is designated as the first baffle plate 48.First baffle plate 48 is vertical baffle plate, the bottom of the first baffle plate 48 and formation gap, support portion 47.Preferably, described solid particle reagent is latex freeze-drying ball agent.The minimum widith of latex freeze-drying ball agent and the diameter of latex freeze-drying ball are greater than the breadth extreme in gap 47, and latex freeze-drying ball agent is stopped by gap 47.

Preferred as one, as shown in Fig. 5 and Figure 18, the first reaction zone 26 also comprises second baffle 50, and second baffle 50 is obliquely installed, and the minimum widith that second baffle 50 and stop part form the second gap 49, gap 49, second is greater than the breadth extreme of solid particle reagent.Second baffle 50 is used for the drainage of solid particle reagent with stop part, enables solid particle reagent enter into the first reaction zone 26 smoothly.As an embodiment, the breadth extreme of latex freeze-drying ball agent and the diameter of latex freeze-drying ball are less than the minimum widith in the second gap 49, and latex freeze-drying ball agent is conveniently entered in the first reaction zone 26.

Preferred as one, the first baffle plate 48 and second baffle 50 are for having the baffle plate of radian.On the one hand solid particle reagent can be entered fast, consider the difference on Solids Particle Diameter, avoid solid particle be stuck between the first baffle plate 48 and second baffle 50 and cannot fall on step 46.

Preferred as one, described reacting part also comprises second reaction zone, and second reaction zone is used for temporary liquid reagent.Preferably, second reaction zone is provided with conducting element, and conducting element comprises the first drainage plate and the second drainage plate.

As a kind of embodiment, as shown in Figure 5 and Figure 6, reacting part comprises the solid particle that the first reaction zone 26 and reaction zone 26, second reaction zone 25, first discharge for receiving reagent container cavity 106, the liquid reagent that second reaction zone 25 discharges for receiving reagent container cavity 108.Second reaction zone 25 is provided with conducting element, and conducting element comprises the first drainage plate 42 and the second drainage plate 44, and liquid reagent is flowed rapidly in second reaction zone 25.First reaction zone 26 comprises step 46, first baffle plate 48 for temporary reagent and second baffle 50, first baffle plate 48 and second baffle 50 enter step 46 for guiding solid particle, simultaneously, gap 47 is formed between first baffle plate 48 and step 46, this gap 47 can stop solid particle to enter second reaction zone 25, and allows liquid reagent to flow in the first reaction zone 26.

As shown in Figure 19 and Figure 19 A, biological test sample box 100 comprises top board 2, base plate 4, left plate 6, right plate 8, panel 10 and backboard 12.Biological test sample box 100 is roughly square box-like body, is made up of plastic material,

Preferred as one, as shown in figure 19, the left plate 6 of described biological test sample box 100 or one end of right plate 8 are an inclined-plane 22.Preferably, the backboard 12 of biological test sample box 100 is provided with an extension 24, and extension 24 extends out to the outside on inclined-plane 22.Preferably, inclined-plane 22 is arranged on the lower left corner of biological test sample box 100.In one embodiment, backboard 12 is square, and panel 10 is pentagon, and left plate 6 has a side and an inclined-plane, and extension 24 is triangle at right angles, and the hypotenuse of right-angle triangle is the junction of backboard 12 and extension 24.The setting of inclined-plane 22 and extension 24, be convenient to the pros and cons artificially distinguishing biological test sample box 100, avoid biological test sample box 100 to be oppositely inserted into and detect in box 200, on the other hand, when biological test sample box coordinates with detection box, automatically can identify the pros and cons of biological test sample box, avoid maloperation.

Preferred as one, as shown in figure 20, biological test sample box 100 is provided with positioning convex 16.Positioning convex 16 arranges on the faceplate 10, positioning convex 16 is in upside-down triangle shape, as Figure 20 A, positioning convex 16 is detecting in the corresponding groove of box 200 for being fixedly positioning by biological test sample box 100, positioning convex 16 engages with groove is spacing, biological test sample box 100 is fastened on detect in box 200, when avoiding rotating, biological test sample box 100 detects the displacement of box 200 relatively.

Preferred as one, as shown in Figure 19 A, the top board 2 of biological test sample box 100 is provided with handle 14, when detecting, facilitate user to hold handle 14 fast biological test sample box 100 to be inserted and detect in box 200, upon completion of the assays, quick again biological test sample box 100 to be extracted.Preferred as one, the backboard 12 of biological test sample box 100 is provided with notch 52, and the setting of notch 52 makes the injection mo(u)lding of biological test sample box 100 more convenient.

As a kind of embodiment, as shown in Fig. 5, Fig. 6 and Figure 22, be provided with reagent storage portion 102, reagent release portion 150, reacting part, detection zone 28, sampling rod 30 and liquid absorption pad 32 in biological test sample box 100, biological test sample box 100, reagent storage portion 102, reagent release portion 104 and sampling rod 30 are made by plastic material.Fluid sample, and to join in biological test sample box 100 as blood, urine etc. for collection liquid sample by sampling rod 30.Upon completion of the assays, liquid absorption pad 32, for Recycling of waste liquid, avoids leak of liquid to pollute.

As shown in Fig. 5, Fig. 6 and Figure 22, biological test sample box 100 is a cavity, box body 18 and upper cover 20 can be divided into, box body 18 comprises backboard 12, base plate 4, left plate 6 and right plate 8, upper cover 20 comprises top board 2 and panel 10, upper cover 20 and box body 18 are tightly connected by the mode such as welding, and facilitate the assembling of biological test sample box 100.Described liquid absorption pad 32 is arranged on the top on inclined-plane 22, turn right the successively direction of side plate 8 of liquid absorption pad 32 is sampling rod 30 and reagent storage portion 102, reacting part and reagent release portion 104 are all arranged on the below in reagent storage portion 102, and detection zone 28 is arranged in reacting part.Sampling rod 30 is arranged in biological test sample box 100 by an opening, and sampling rod 30 coordinates with this opened gap, when avoiding sampling, and being mixed into of foreign matter.Inclined-plane 22 is arranged on the left of sampling rod 30, and the sample be conducive on sampling rod 30 fully contacts with reactant liquor, avoids reactant liquor very few and does not reach sample.The general layout of liquid absorption pad 32, reagent storage portion 102, sampling rod 30, reacting part and reagent release portion 104 is arranged and is not limited to upper type.Preferably, the second drainage plate 44 is connected with the first baffle plate 48 upper end, and the upper end of the first drainage plate 42 is also connected with sampling needle guide plate 57, and sampling needle guide plate 57 vertical direction is arranged.As shown in Figure 12 and Figure 20 B, box body 18 and upper cover 20 are equipped with sampling needle fixture 59, for sampling needle fixture 59, avoid it to rock.

As a kind of embodiment, reagent storage portion and biological test sample box connected mode, as shown in Figure 8 A, reagent storage portion 102 is provided with keeper, each keeper constitutes cavity, this cavity, for accommodating reagent storage portion 102, makes reagent storage portion 102 be fixedly mounted in biological test sample box 100.Described cavity also can adopt routine techniques means of the prior art.As a specific embodiment, as shown in Figure 12 and 12A, Figure 20 and Figure 20 B, panel 10 is provided with reference column 54 and back up pad 61, left positioner plate 56 and right positioner plate 58 are the keeper of multiple dispersion, scattering device is on box body 18 and upper cover 20, and above-mentioned keeper is used for being fixedly connected with reagent storage portion 102.As shown in Figure 8 A and 8 B, the keeper in reagent storage portion 102 comprises mounting hole 122, left keeper 124, right keeper 126 and lower keeper 128.As shown in figure 21, mounting hole 122 is buckled on reference column 54, for the upper end of fixating reagent storage part 102; Left keeper 124 and right keeper 126 are respectively with left positioner plate 56 with right positioner plate 58 is spacing is connected, and lower keeper 128 is placed in back up pad 61.Therefore, what reagent storage portion 102 was firm is arranged in biological test sample box 100, avoids reagent storage portion 102 and moves at biological test sample box 100 or rock the displacement in process.

Above-mentioned each preferably and between embodiment can be matched arbitrarily as required and combine, to realize the object of final quick sample Concentration Testing.

As shown in figure 23 and figure 24, detect in box 200 and be provided with a push rod 204, push rod 204 to be contacted with forced section by the described opening 34 on biological test sample box and provides the acting force of external unit.

Preferred as one, described push rod 204 relatively can detect box 200 and move.Push rod 204 can fixedly be positioned on box body, also can with box body relative motion.When push rod 204 is movable, by the zone of action of peripheral equipment control push rod 204 and the position of movement, such as, with the movement of Electric Machine Control push rod 204, also can adopt routine techniques of the prior art.If push rod 204 is fixed on box body, when biological test sample box 100 inserts the detection box 200 of external unit, by insertion force, push rod 204 is coordinated with push rod 152, make push rod 152 action.

Preferred as one, described push rod 204 be arranged on detect box 200 base plate on or described push rod 204 be arranged on detect box 200 inner panel on.As shown in figure 25, when detection box 200 is the bottom surface of push rod 152 with forced section, opening 34 is arranged on the base plate 4 of biological test sample box 100, and push rod 204 is arranged on the base plate of detection box 200.Preferably, the upper end of push rod 204 is provided with chamfering, facilitates push rod 204 to stretch into opening 34.When the back that detection box 200 and forced section are push rod 152 coordinates, opening 34 is arranged on the backboard 12 of biological test sample box 100, and push rod 204 is arranged in the inner panel of detection box 200.

Preferred as one, as shown in Figure 24 and Figure 26, detect in box 200 and be provided with a portable plate, portable plate comprises substrate 206 and elastic component 210, and substrate 206 is fixedly connected on the inwall of detection box 200 by elastic component 210.Preferably, the quantity of elastic component 210 is two, and elastic component 210 comprises installed surface 212 and two elastic arms 214, and installed surface 212 is fixedly connected with the medial surface detecting box 200, and two elastic arms 214 are fixedly connected with substrate 206 respectively.

Preferred as one, described substrate 206 is heating plate, is designated as the first heating plate.Namely not only portable plate is as the while of heating member but also as elastic fastener.

Preferred as one, one of them medial surface of described detection box 200 is provided with shell fragment 216 as shown in figures 23-27.Preferably, shell fragment 216 is arranged on one of them medial surface adjacent with portable plate.Shell fragment 216 is provided with an elastic arm 218, and elastic arm 218 one end and shell fragment 216 are fixed, and the other end of elastic arm 218 is smooth curved surface shape, and elastic arm 218 is installed towards detection box 200 internal cavities.Shell fragment 216, in order to coordinate the inclined-plane 22 on biological test sample box 100, makes side recoil case 100 not being arranged inclined-plane be close to the madial wall detecting box 200.Because shell fragment 216 has certain elasticity, if when biological test sample box 100 is oppositely inserted into and detects in box 200, due to the effect of shell fragment 216, biological test sample box 100 cannot plug together completely in detection box 200, therefore, the cooperation on shell fragment 216 and inclined-plane 22 can play again the recognition reaction that biological test sample box 100 correctly inserts.

Preferred as one, as shown in figure 23, one of them medial surface of described detection box 200 is provided with groove 202.Preferably, groove 202 is arranged on the medial surface of relative motion plate.Groove 202, for the positioning convex 16 on clamping biological test sample box 100, makes biological test sample box 100 can be connected on groove 202, avoids and detects box 200 in rotation or vibration processes, the displacement of biological test sample box 100.

As a kind of embodiment, as shown in Fig. 4, Figure 23 and Figure 24, detecting box 200 is fixed on external unit, to realize mixing or to rotate, detect the box body that box 200 comprises open at one end, box body is provided with light hole 201, push rod 204, first heating plate 206 and the second heating plate 208 is provided with in box body, first heating plate 206 is portable plate, push rod 204 to be contacted with forced section by above-mentioned opening 34 and provides external thrust, first heating plate 206 and the second heating plate 208 for adding thermal agent, to meet the requirement of temperature of reaction.

As shown in figure 25, when biological test sample box 100 is inserted in detection box 200, biological test sample box 100 presses the first heating plate 206, first heating plate 206 under pressure, make elastic component 210 that deformation occur, biological test sample box 100 can quick insertion being fastened in groove 202 by positioning convex 16.Meanwhile, the inclined-plane 22 of biological test sample box 100 compresses shell fragment 216, makes the detection zone 28 on biological test sample box 100 aim at the light hole 201 detected on box 200.Be inserted at biological test sample box 100 in the process detecting box 200, push rod 204 stretches into opening 34 and contacts with push rod 152, along with in the basipetal process of biological test sample box 100, push rod 152 action from lower to upper, the action from lower to upper of band dynamic seal 104, seal 104 and reagent storage portion 102 are torn off, and release reagent as shown in Figure 7 A.

On existing market face, glycosylated hemoglobin detection method is a lot, and wherein conventional detection method has ion-exchange chromatography, affinity chromatography, high pressure liquid phase, immunization, Ion capture, electrophoresis etc.After immunization refers to erythrocytolysis, HbA1c is measured in the interaction based on antigen molecule and distinct antibodies.

Detect HbA1c by immune agglutination method and comprise following two detecting steps: detect the total hemoglobin Hb concentration in sample and Glycohemoglobin HbA1c concentration respectively.The detection of total hemoglobin (Hb) is by using the ferrous ion in potassium ferricyanide oxygenated haemoglobin, generate methemoglobin, methemoglobin and rhodanide reaction generate thiocyanic acid methemoglobin, detect the light absorption value under 531nm, obtain the concentration of Hb.Glycosylated hemoglobin (HbA1c) is marked at the anti-HbA1c antibody on latex beads with the HbA1c competition binding in the agglutinin containing multiple HbA1c immune response binding site and blood, the former combination can induce reaction the change of liquid turbidity, can be obtained the concentration of HbA1c in blood by the light absorption value change detecting 531nm.In blood, the concentration of HbA1c is also high, and turbidity is lower, and light absorption value is less, and the change of light absorption value and HbA1c concentration is obtained by calibration curve.

Therefore, immunization detects HbA1c needs to use thiocyanate liquid reagent (Buffer), the latex balloon that marked anti-HbA1c antibody, potassium ferricyanide dried reagent (drying object) and the agglutinin dried reagent (drying object) containing multiple HbA1c immune response binding site.As shown in figure 28, thiocyanate liquid reagent 60 is stored in reagent container cavity 108, the latex balloon 62 that marked anti-HbA1c antibody is stored in reagent container cavity 106, and potassium ferricyanide dried reagent 64 is solidificated on second reaction zone 25, and agglutinin dried reagent 66 solidifies in the first reaction zone 26.

Wherein, latex balloon is latex freeze-drying bead; upper specificity HbA1c antibody is connected by covalently bound method in advance by latex bead; freeze drying technology is adopted to be the bead that volume is identical by latex balloon quick-frozen again; achieve the maximization to the protection of latex antibody reactivity; meanwhile, improve term of validity storage life under normal temperature greatly.

Biological test sample box of the present utility model is utilized to detect the concentration of HbA1C in blood.The step that immunization detects HbA1c is as follows:

Step 0: as shown in figure 28, is inserted into the sampling rod 30 with sample in biological test sample box.

Step 1: as shown in Figure 28 A, rotate and detect box, biological test sample box is inserted into and detects in box, push rod 152 moves upward under the effect of external thrust, tear off seal 104, latex balloon 62 and thiocyanate 60 discharge simultaneously, and latex balloon 62 falls and to enter in the first reaction zone 26 temporary, and thiocyanate liquid reagent 60 falls and to enter in second reaction zone 25 in detection zone 28.

Step 2: as shown in Figure 28 B, rotating biological sample test box, mixes Buffer60 (thiocyanate), drying object 64 (potassium ferricyanide) and blood sample, forms mixture X.

Step 3: as shown in fig. 28 c, rotating biological sample test box, rotates the mixture X in step 2 to detection zone 28, detects the content of haemoglobin (Hb) in sample.

Step 4: as shown in fig. 28d, continue rotating biological sample test box, the mixture X in step 2 is made to enter the first reaction zone 26, potpourri mixes with drying object 66 (agglutinin containing multiple HbA1c immune response binding site) and latex balloon 62 (marked the latex beads of anti-HbA1c antibody), forms potpourri Y.

Step 5: as shown in Figure 28 E, rotating biological sample test box, makes the potpourri Y in step 4 enter detection zone 28, detects the content of glycosylated hemoglobin (HbA1C) in sample.

Step 6: as shown in Figure 28 F, rotating biological sample test box, makes the potpourri Y in step 4 enter liquid absorption pad 32, reacted waste liquid is absorbed.

HbA1c detects end, calculates detection architecture and export by external unit.

Biological test sample box of the present utility model is not limited to the detection of HbA1c in above-mentioned blood sample, also may be used for the detection of other biological sample as urine, saliva, spinal fluid etc., also can be used for the Concentration Testing that C reactive protein in biological specimen, cholesterol, blood fat and blood sugar etc. analyze thing.

In above-mentioned explanation, all special instructions that do not add, all adopt routine techniques means of the prior art.

Claims

1. a biological test sample box, it is characterized in that, being provided with one in described testing cassete can the push rod of testing cassete motion and the chute that is slidably matched with push rod relatively, at least one side of described push rod is provided with at least one spacing preiection, and chute is provided with the limited impression engaged with described spacing preiection.

2. biological test sample box according to claim 1, is characterized in that, described spacing preiection is two, is separately positioned on the left and right side of push rod.

3. biological test sample box according to claim 1, is characterized in that, described spacing preiection is arranged on the top of push rod side.

4. biological test sample box according to claim 1, is characterized in that, the described push rod place of keeping to the side is provided with the cell body of at least one hollow out, and described spacing preiection is arranged on the lateral wall of cell body, and spacing preiection and cell body are arranged in pairs.

5. biological test sample box according to claim 1, is characterized in that, described push rod is provided with a groove.

6. biological test sample box according to claim 1, is characterized in that, described push rod is all housed in testing cassete.

7. biological test sample box according to claim 1, is characterized in that, described chute is provided with division board, the inner space of division board isolation chute and testing cassete.