CN203490223U - Joint detection reagent card for ferritin and bone alkaline phosphatase in human blood - Google Patents
Joint detection reagent card for ferritin and bone alkaline phosphatase in human blood Download PDFInfo
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- CN203490223U CN203490223U CN201320544153.3U CN201320544153U CN203490223U CN 203490223 U CN203490223 U CN 203490223U CN 201320544153 U CN201320544153 U CN 201320544153U CN 203490223 U CN203490223 U CN 203490223U
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- ferritin
- alkaline phosphatase
- bone alkaline
- test paper
- paper core
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Abstract
The utility model discloses a joint detection reagent card for ferritin and bone alkaline phosphatase in human blood and belongs to the field of detection of proteins, peptides or amino acids. The joint detection reagent card comprises a plastic shell which is provided with a sample feeding hole, a diluting liquid adding hole and a detection window, wherein a joint detection test paper core for the ferritin and the bone alkaline phosphatase in the human blood is inserted into the plastic shell; the test paper core comprises a bottom plate, a nitrocellulose membrane, a marker cushion and water adsorption paper, wherein one end of the bottom plate is stuck with a sampling cushion; the nitrocellulose membrane is coated with a first detection strip belt, a second detection strip belt and a quality control strip belt; the marker cushion and the water adsorption paper are respectively overlapped at the two ends of the nitrocellulose membrane. The joint detection reagent card can be used for simultaneously detecting the ferritin and the bone alkaline phosphatase in the blood; the detection sensitivity and the specificity can meet the clinical diagnosis requirement and the operation process is simplified.
Description
Technical field
The utility model relates to ferritin and bone alkaline phosphatase combined detection reagent card in protein, peptide or amino acid whose detection, particularly a kind of blood of human body.
Background technology
Ferritin is as a kind of albumen of storing iron in body, and its serum (blood plasma) concentration represents body iron deposit situation.When infecting or some tumour occurs, serum (blood plasma) ferritin concentration also can increase extremely as acute phase reactive protein.Generally, serum ferritin concentration is at 20 to 200 ug/L, i.e. 20-200ng/ml.
At present, the main detection method of serum (blood plasma) Ferritin Levels comprises radioimmunology, euzymelinked immunosorbent assay (ELISA) and chemoluminescence method etc.Above-mentioned these detection methods all need to rely on the instrument and equipment of corresponding complexity, also need to use liquid reagent, therefore, have trace routine complexity, a defect such as detection speed is slow and testing result is unreliable.In addition, the required sample size of these methods is larger, all wants venous blood samples to detect, and is also not easy to be accepted by children.
Calcium deficiency is the especially common nutritional deficiency disease of children of population of China.Serum (blood plasma) bone alkaline phosphatase concentration is significant to auxiliary diagnosis calcium deficiency.At present, detect bone alkaline phosphatase concentration and generally adopt euzymelinked immunosorbent assay (ELISA), electrophoresis, immune concentrating chemical method.First two method need detect from venous blood sampling separation of serum (blood plasma), be difficult for being accepted by children, and testing process complicated operation, time is long; The error of rear a kind of method testing result is larger, can not draw the result of quantitative measurement, and also has the weakness of complex operation.
Utility model content
The purpose of this utility model is in order to solve the problems of the technologies described above, and has designed ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body.
The utility model is realized according to following technical scheme:
Ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body, by test paper core and plastic housing, formed, test paper core comprises the loading pad that mutually overlaps successively and be pasted on base plate, labeling pad, nitrocellulose membrane and suction sample pad, plastic housing comprises base and panel, wherein base middle part is formed with and places the support structure of test paper core and a plurality of column type draw-in grooves of periphery, on panel, form and be provided with the detection window corresponding with cellulose nitrate film location size in test paper core, the well corresponding with loading pad position and a plurality of column type fixture block, described support structure comprises and is positioned at base centre and the brace summer equal in length with test paper core, the reinforcement that a plurality of and brace summer intersects vertically and the fluted body position limiting structure and the Flapper type position limiting structure that are positioned at brace summer two ends, on described panel, be also provided with corresponding with loading pad position in the test paper core dilution hole that adds, on described nitrocellulose membrane, be arranged with successively in order coated the first test strip forming of ferritin monoclonal antibody in parallel, coated the second test strip forming of bone alkaline phosphatase polyclonal antibody and the coated Quality Control band forming of sheep anti-mouse igg.
Utilize ferritin and bone alkaline phosphatase combined detection reagent card in the blood of human body that the technical solution of the utility model makes, detect two kinds of indexs simultaneously, simplified running program, save time, and sensitivity and specificity can meet clinical diagnosis requirement completely, be applicable to the fast detecting of ferritin and bone alkaline phosphatase.And the utility model adopts Gold-immunochromatography assay quantitative measurement technology, with 20 microlitre blood, can detect serum (blood plasma) ferritin and bone alkaline phosphatase concentration simultaneously, being conducive to take to point blood measures, easily by child patient, accepted, also simplify test operating procedure, shortened the test duration.
Accompanying drawing explanation
Fig. 1 is the perspective view of base in the utility model;
Fig. 2 is the perspective view of card faceplate in the utility model;
Fig. 3 is the perspective view of the utility model pilot scale refill.
Wherein: 1a, base 1b, panel
2, support structure 2a, brace summer
2b, reinforcement 2c, Flapper type position limiting structure
2d, fluted body position limiting structure 3, column type draw-in groove
4, detection window 5, well
6, add dilution hole 7, column type fixture block
8, base plate 9, loading pad
10, labeling pad 11, nitrocellulose membrane
12, inhale sample pad 13, the first test strip
14, the second test strip 15, Quality Control band.
Embodiment
Below in conjunction with drawings and Examples, the utility model is specifically described.
Ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body, by test paper core and plastic housing, formed, test paper core comprises the loading pad 9 that mutually overlaps successively and be pasted on base plate 8, labeling pad 10, nitrocellulose membrane 11 and suction sample pad 12, plastic housing comprises base 1a and panel 1b, wherein base 1a middle part is formed with and places the support structure 2 of test paper core and a plurality of column type draw-in grooves 3 of periphery, the upper formation of panel 1b is provided with the detection window 4 corresponding with the 11 position sizes of nitrocellulose membrane in test paper core, the well 5 corresponding with loading pad 9 positions and a plurality of column type fixture block 7, described support structure 2 comprises and is positioned at base 1a centre and the brace summer 2a equal in length with test paper core, the reinforcement 2b that a plurality of and brace summer 2a intersects vertically and the fluted body position limiting structure 2d and the Flapper type position limiting structure 2c that are positioned at brace summer 2a two ends, on described panel 1b, be also provided with corresponding with loading pad 9 positions in the test paper core dilution hole 6 that adds, on described nitrocellulose membrane 11, be arranged with successively in order coated the first test strip 13 forming of ferritin monoclonal antibody in parallel, coated the second test strip 14 forming of bone alkaline phosphatase polyclonal antibody and the coated Quality Control band 15 forming of sheep anti-mouse igg.
One end of described labeling pad 10 and nitrocellulose membrane 11 are near a side overlap joint of the first test strip 13, and the other end and loading pad 9 overlap.
Described suction sample pad 12 and the side overlap joint of nitrocellulose membrane 11 near Quality Control band 15.
Support structure 2 on described base 1a is agreed with mutually with test paper core size.Brace summer 2a in support structure 2 and reinforcement 2b slow down the molecular diffusion that joins the sample on test paper core, make the chemical reaction of the collaurum on sample and test paper core more fully thoroughly, the Flapper type position limiting structure 2c that described brace summer 2a two ends arrange respectively and fluted body position limiting structure 2d, be fixed in test card base 1a test strips by described position limiting structure.
Column type draw-in groove 3 on described base 1a matches with column type fixture block 7 sizes on panel 1b, and position is symmetrical, and base 1a and panel 1b are fixed by column type draw-in groove 3 and column type fixture block 7.
The preparation of labeling pad 10: prepare by gold chloride-trisodium citrate reduction method the colloidal gold solution that diameter is 30-50nm, get 100ml colloidal gold solution after having prepared, use 0.2MK
2cO
3pH value of solution is transferred to pH9.0, then solution is placed on magnetic stirring apparatus and slowly stirs, by every 100ml solution, add 0.5mg ~ 1.5mg ferritin monoclonal antibody and bone alkaline phosphatase antibody, be slowly added drop-wise in colloidal gold solution, continue to stir 1 hour, adding final concentration is 0.2% BSA again, 0.1% PEG 20000 seals 30 minutes, and 12000 leave the heart 30 minutes, abandon supernatant, with collaurum working fluid, redissolve to 76.5ml, by 1ml solution paving 16cm
2ratio be layered on nonwoven fabrics equably, then be placed in 20～25 ℃ of temperature, humidity and be less than 30% drying room dry 2～4 hours, make labeling pad 10.
Being coated with of ferritin monoclonal antibody and bone alkaline phosphatase polyclonal antibody: the ferritin monoclonal antibody solution after suitably diluting is pressed to 1.0ul/cm line with spray film instrument on nitrocellulose membrane and be coated with, formation the first test strip 13; Bone alkaline phosphatase Anti-TNF-α liquid solution after suitably diluting is pressed to 1.0ul/cm line with spray film instrument on nitrocellulose membrane and be coated with, formation the second test strip 14; Finally coated sheep anti-mouse igg antibody, as Quality Control band 15 on nitrocellulose membrane, after being coated with is placed in nitrocellulose membrane 11 drying room dry 3～4 hours.
The assembling of ferritin and bone alkaline phosphatase joint-detection test paper core in blood of human body: in 20～25 ℃ of temperature, humidity are less than 30% hothouse, get base plate 8, paste at the middle part that coated nitrocellulose membrane 11 is placed on to base plate 8, at nitrocellulose membrane 11, near the first test strip 13 1 sides, overlap labeling pad 10, at labeling pad 10 opposite side overlap joints, paste loading pad 9, in nitrocellulose membrane 11 Quality Control band 15 1 sides, apart from overlapping edges, inhale sample pad 12; Then with cutter, the base plate posting is cut into the test paper core of proper width.
The utility model is when reality detects, first tearing packaging foil bag taking-up reagent card keeps flat, getting 20 microlitres drips sample drop and adds in well 5, in adding dilution hole 6, drip immediately two dilutions, static 15 minutes, with immunity-chromatography test interpretative instrument, analyze the first test strip 13 and the second test strip 14 profiles and shade, according to corresponding typical curve, calculate the amount of ferritin and the bone alkaline phosphatase of these two line representatives.If redfree nature controlling line occurs, illustrates that this experiment is invalid.
Utilize ferritin and bone alkaline phosphatase combined detection reagent card in the blood of human body that the technical solution of the utility model makes, detect two kinds of indexs simultaneously, simplified running program, save time, and sensitivity and specificity can meet clinical diagnosis requirement completely, be applicable to the fast detecting of ferritin and bone alkaline phosphatase.And the utility model adopts Gold-immunochromatography assay quantitative measurement technology, with 20 microlitre blood, can detect serum (blood plasma) ferritin and bone alkaline phosphatase concentration simultaneously, being conducive to take to point blood measures, easily by child patient, accepted, also simplify test operating procedure, shortened the test duration.
Claims (5)
1. ferritin and bone alkaline phosphatase combined detection reagent card in a blood of human body, by test paper core and plastic housing, formed, test paper core comprises the loading pad (9) that mutually overlaps successively and be pasted on base plate (8), labeling pad (10), nitrocellulose membrane (11) and suction sample pad (12), plastic housing comprises base (1a) and panel (1b), wherein base (1a) middle part is formed with and places the support structure (2) of test paper core and a plurality of column type draw-in grooves (3) of periphery, the upper formation of panel (1b) is provided with the detection window (4) corresponding with nitrocellulose membrane (11) position size in test paper core, the well (5) corresponding with loading pad (9) position and a plurality of column type fixture block (7), it is characterized in that: described support structure (2) comprises and is positioned at base (1a) centre and the brace summer (2a) equal in length with test paper core, a plurality of reinforcement (2b) and the fluted body position limiting structure (2d) that is positioned at brace summer (2a) two ends and Flapper type position limiting structures (2c) that intersect vertically with brace summer (2a), on described panel (1b), be also provided with corresponding with loading pad (9) position in the test paper core dilution hole (6) that adds, on described nitrocellulose membrane (11), be arranged with successively in order coated the first test strip (13) forming of ferritin monoclonal antibody in parallel, coated the second test strip (14) forming of bone alkaline phosphatase polyclonal antibody and the coated Quality Control band (15) forming of sheep anti-mouse igg.
2. ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body according to claim 1, it is characterized in that: one end of described labeling pad (10) and nitrocellulose membrane (11) are near a side overlap joint, the other end and loading pad (9) overlap joint of the first test strip (13).
3. ferritin and bone alkaline phosphatase combined detection reagent card in blood of human body according to claim 1, is characterized in that: described suction sample pad (12) and the side overlap joint of nitrocellulose membrane (11) near Quality Control band (15).
4. ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body according to claim 1, is characterized in that: the support structure (2) on described base (1a) is agreed with mutually with test paper core size.
5. ferritin and bone alkaline phosphatase combined detection reagent card in a kind of blood of human body according to claim 1, it is characterized in that: the column type draw-in groove (3) on described base (1a) matches with column type fixture block (7) size on panel (1b), position is symmetrical, base (1a) and panel (1b) being fixed by column type draw-in groove (3) and column type fixture block (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320544153.3U CN203490223U (en) | 2013-09-03 | 2013-09-03 | Joint detection reagent card for ferritin and bone alkaline phosphatase in human blood |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320544153.3U CN203490223U (en) | 2013-09-03 | 2013-09-03 | Joint detection reagent card for ferritin and bone alkaline phosphatase in human blood |
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| CN203490223U true CN203490223U (en) | 2014-03-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320544153.3U Expired - Fee Related CN203490223U (en) | 2013-09-03 | 2013-09-03 | Joint detection reagent card for ferritin and bone alkaline phosphatase in human blood |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353261A (en) * | 2016-08-31 | 2017-01-25 | 长沙励思生物技术有限公司 | Bone alkaline phosphatase analyzer |
| CN109295201A (en) * | 2018-09-29 | 2019-02-01 | 中国航天员科研训练中心 | Predict the method and DNA methylation marker of weightless bone loss bone alkaline phosphatase BALP downside risk |
| CN109459568A (en) * | 2019-01-15 | 2019-03-12 | 西安交通大学 | Paper base periodontitis detection device and periodontitis detection method |
| CN109475869A (en) * | 2016-07-18 | 2019-03-15 | 西门子医疗保健诊断公司 | Fluid analysis reagent distributing equipment and its relevant analysis kit and application method |
-
2013
- 2013-09-03 CN CN201320544153.3U patent/CN203490223U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109475869A (en) * | 2016-07-18 | 2019-03-15 | 西门子医疗保健诊断公司 | Fluid analysis reagent distributing equipment and its relevant analysis kit and application method |
| US11040350B2 (en) | 2016-07-18 | 2021-06-22 | Siemens Healthcare Diagnostics Inc. | Liquid analytical reagent dispensing apparatus and analytical kits and methods of use related thereto |
| CN109475869B (en) * | 2016-07-18 | 2021-07-30 | 西门子医疗保健诊断公司 | Liquid assay reagent dispensing apparatus and related assay kits and methods of use |
| CN106353261A (en) * | 2016-08-31 | 2017-01-25 | 长沙励思生物技术有限公司 | Bone alkaline phosphatase analyzer |
| CN109295201A (en) * | 2018-09-29 | 2019-02-01 | 中国航天员科研训练中心 | Predict the method and DNA methylation marker of weightless bone loss bone alkaline phosphatase BALP downside risk |
| CN109459568A (en) * | 2019-01-15 | 2019-03-12 | 西安交通大学 | Paper base periodontitis detection device and periodontitis detection method |
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| GR01 | Patent grant | ||
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Granted publication date: 20140319 Termination date: 20170903 |