CN201892653U - Leucocyte subgenera analysis multidimensional optical information sensor - Google Patents

Abstract

The utility model relates to an analysis equipment of particulate matter physical characteristics, in particular to a leucocyte subgenera analysis multidimensional optical information sensor. The sensor comprises a laser light source, a first focusing lens set, a diaphragm, a double-horn mouth flowing type pipe, a light trap, a bidimensional composite focusing system formed by a hollow ellipsoidal reflecting surface along the vertical direction of an incident light and a second focusing lens set, a diaphragm, a photoelectric detector, a bidimensional beam-splitting focusing system formed by a lens which is forwards along the incident light and a plane mirror, a diaphragm and a photoelectric detector. The sensor can be used for integrally acquiring a leucocyte light scattering universal transient state optical information and converting the information into an electric signal, so that optical information is overall and abundant, the structure of an optical information acquiring system is compact, the application of optical lenses is flexible, the light noise is low, the integral acquisition of the four-dimensional information is realized, and the physical characteristics of leucocytes can be wholly reflected.

Description

A kind of leucocyte subclass is analyzed multidimensional optical information sensor

Technical field

The utility model relates to a kind of analytical equipment of various particle properties, refers in particular to a kind of leucocyte subclass and analyzes multidimensional optical information sensor.

Background technology

The detection technique of form particulate (particularly biological cell) physical features is the science and technology that technical field more than is intersected arbitrarily, the form particulate mainly contains dust particle arbitrarily, biological cell and arena particulate etc., its feature more complicated, principal character has size distribution, form distributes, nuclear formula structure or the like, optical sensing methods mainly is exactly to utilize the relation of the Strength Space distribution characteristics of light scattering and particulate physical features to come the classification of detection of particles, wherein key is the conversion of effective photosignal, patented technology at special particulate has some application at present, for example patented technology 200710192385 .6 (optical sensor of high-flux dust particle counter) have designed spheric reflection formula lateral light information sensing mode at dust particle, but this information can not reflect the physical features of particulate comparatively all sidedly, and scattering source and receiving station are more to the loss of signal so all not on focus; Patented technology CN92215120.2(laser dust particle counter novel optical probe and for example) adopted the reflective lateral light information sensing of ellipsoid mode, though scattering source and receiving station are all on focus, but the light scattering meeting at the little angle of side direction once more with the particulate effect, produce multiple scattering, influence the accuracy of information; The patented technology CN101498646A(low-angle forward direction laser light scattering sensor that is used for leucocyte three classification for another example), it is photelectric receiver that this technology adopts diode array, cylindrical mirror is processed light beam, obtained the light scattering information of the little angle of forward direction and the forward direction angle of elevation, but the side information for sensitivity reflection cell interior structure is not gathered, so accurately discern the limited in one's ability of multiclass cell; Also just like United States Patent (USP) technology 2004/0197232/A1(back scattering to blood of human body cell recognition device), this technology is used fiber array sensing mode from forward direction and back to obtaining the particulate scattered information, improve cell effectively and adopted the accurate degree of identification, but backscatter signal is extracted more complicated, and do not gather with nucleus and the closely-related side direction polarization information of molecular structure, therefore cell particulate identification classification is not strong, in addition, the companies such as Abbott Laboratories of some enterprises such as the U.S. both at home and abroad, the Sysmex of Japan also has company such as Chinese Mai Rui that Application and Development patented technology (as: the MDOS four angle laser patent measuring techniques of some light scattering techniques are arranged in leukocytic identification detector device; Laser method Flow Cytometry five classification etc.), but mainly all be to be equipped with The Application of Technology such as chemistry, electronics, fluorescence, and the optical sensing mode is single, the utility model is a kind of pure optics, and a kind of that optimize and combine, the patented technology with particulate multidimensional optical information sensing of the widespread usage meaning improved at the deficiency of using in the practice.

Summary of the invention

The purpose of the utility model patent is to provide a kind of information completely, compact conformation, signal to noise ratio (S/N ratio) height, leucocyte subclass that particulate classification recognition capability is strong to analyze multidimensional optical information sensor.

The utility model technical solution is: the leucocyte subclass is analyzed multidimensional optical information sensor, and it is characterized in that: described sensor comprises laser instrument, first focus lens group, first diaphragm, double horn mouth streaming pipe, second diaphragm, first photodetector, second focus lens group, the 3rd diaphragm, hollow ellipsoid reflecting surface, polaroid, second photodetector, first spherical mirror, catoptron, second spherical mirror, the 4th diaphragm, the 3rd photodetector, the 3rd spherical mirror, the 5th diaphragm, the 4th photodetector, light trapping and seal closure; First diaphragm, second diaphragm, the 3rd diaphragm, the 4th diaphragm and the 5th diaphragm are opened on the seal closure; Laser instrument, first focus lens group, first diaphragm, double horn mouth streaming pipe, light trapping, first spherical mirror, catoptron, second spherical mirror, the 4th diaphragm and the 3rd photodetector are positioned on the same incident light axis successively according to the direction of laser incident, and double horn mouth streaming pipe is placed perpendicular to incident light axis and with a tight waist being centered close on the incident light axis; Second photodetector, polaroid, the 3rd diaphragm, second focus lens group, hollow ellipsoid reflecting surface, double horn mouth streaming pipe, second diaphragm and first photodetector are positioned on the same side scattered light axle, second focus lens group is arranged in the vacancy of hollow ellipsoid reflecting surface, and lateral scattering optical axis and incident light axis intersect at the center with a tight waist of double horn mouth streaming pipe; Catoptron, the 3rd spherical mirror, the 5th diaphragm and the 4th photodetector are positioned on the forward scattering optical axis, and scattering optical axis and incident light axis intersect vertically; First spherical mirror and second spherical mirror constitute the 3rd condenser lens group, and catoptron is positioned at the 3rd condenser lens group center, first spherical mirror and the 3rd spherical mirror and constitutes the 4th focus lens group.Entire device is arranged on the seal closure.

Forward direction in LASER Light Source has the first focus lens group and a pair of hydraucone streaming pipe that is positioned at the focus place, the neck of this streaming pipe is positioned at the perifocus district of laser beam, exist the flow field of velocity gradient to have the effect of constraint particulate at the streamline middle part, the focal area is provided with light trapping; Scattered light from the big solid angle of focus one place's particulate side direction can be focused on first photodetector that is positioned at behind focus two place's diaphragms along the hollow ellipsoid reflecting surface of incident light vertical direction, particulate and photodetector are respectively on two focuses of hollow ellipsoid reflecting surface, and second focus lens group that is arranged in hollow ellipsoid reflecting surface vacancy focuses on the laser beam of the little solid angle of side direction on second photodetector at this focus place by this focus lamp group, diaphragm and polaroid; Along the incident light forward direction, big solid angle scattered beam focuses on the 3rd photodetector behind the diaphragm by the 3rd condenser lens group, and the plane mirror at lens combination middle part redirect to the scattered beam of little solid angle in the 4th focus lamp group and focuses on the 4th photodetector that is positioned at behind the diaphragm.

The effective quartz ampoule of described streaming is made, and the intermediate hollow pipeline structure is by mutual inverted double horn interruption-forming, at the thin place of neck because the velocity flow profile feature in flow field can retrain particulate at the streamline middle part, can counterflush during obstruction, guarantee that stream normally uses.

Described hollow ellipsoid reflecting surface can focus on the scattered light of the big solid angle in focus one place on the photodetector at focus two places effectively, because the light at middle part do not reflect, thereby has effectively avoided the influence of rescattering; The second focus lens group that is arranged in hollow ellipsoid reflecting surface vacancy can focus on the laser beam of the little solid angle of side direction on second photodetector at focus place by this focus lamp group, diaphragm and polaroid, to obtain the polarized light information at the little angle of side direction.

And along the incident light forward direction, formed both direction by lens, plane mirror and lens, the large and small angle third and fourth focus lamp group along separate routes, compact conformation, forward scattering light can be focused on respectively by large and small solid angle and be positioned on corresponding third and fourth photodetector in each focus place, obtain the large and small angle of forward scattering light scattering information respectively.

The utility model patent with have technology to compare its significant advantage now to be: 1, the Global Information collection capacity is complete, according to the particulate light scattering theory, all can collect with the closely-related forward direction of particle property large and small angle optical information and the large and small angle of side direction optical information (comprising side direction delustring information), therefore but the classification of detection of particles is many, and the degree of accuracy height; 2, information acquisition and sensing arrangement compactness, integrated strong, all the device element is arranged in the seal closure, and is easy to adjust, disturbs few.Optical information acquisition elements applying flexible, different characteristic information adopts different light collection element, can effectively improve information sensing efficient; 3, in the whole optical texture,, be provided with light trapping and diaphragm respectively, can improve the signal to noise ratio (S/N ratio) of sensor in the place of each correspondence; 4, the double horn mouth streaming tubular construction of photosensitive area uniqueness both can rely on the constraining force of fluid that particulate is controlled at stream pipe middle part, was beneficial to counterflush conducting when stream pipe plug plug again.

Description of drawings

Fig. 1 is a structural representation of the present utility model;

Fig. 2 is the structural representation of streaming pipe in the utility model;

1, laser instrument 2, first focus lens group 3, first diaphragm 4, double horn mouth streaming pipe 5, second diaphragm, 6, first photodetector 7, second focus lens group 8, the 3rd diaphragm 9, hollow ellipsoid reflecting surface 10, polaroid 11, second photodetector, 12, first spherical mirror 13, catoptron 14, second spherical mirror, 15, the 4th diaphragm 16, the 3rd photodetector 17, the 3rd spherical mirror 18, the 5th diaphragm 19, the 4th photodetector 20, light trapping 21, seal closure.

Embodiment

In conjunction with figure one, figure two, it is a vertical bidimensional light path system that the utility model leucocyte subclass is analyzed multidimensional optical information sensor, includes illuminator, fringe area streaming system, bidimensional complex focusing system, bidimensional beam split focusing system composition.Illuminator is made up of laser instrument 1, first focus lens group 2, first diaphragm 3 and light trapping 20, and laser beam is set up an area of illumination at streaming pipe middle part after first focus lens group, cross area of illumination focusing place luminous energy and absorbed by light trapping; Fringe area streaming system is the streaming pipe by the inversion double horn mouth structure of a quartz material, place vertical with laser beam, particulate is subjected to suction function and flows through area of illumination generation light scattering along pipeline in pipe, particulate fluid internal cause hydraucone structure produce the constraining force effect under have the effect of focusing, particulate is controlled at stream pipe middle part, is inverted another hydraucone structure and is beneficial to counterflush conducting when stream pipe plug plug; Bidimensional complex focusing system is made up of hollow ellipsoidal reflector 9, second focus lens group 7, the 3rd diaphragm 8, polaroid 10, second photodetector 11, second diaphragm 5, first photodetector 6, collects vertical direction big angle and 90 degree direction little angle polarization bidimensional optical informations and is converted to electric signal; Bidimensional beam split focusing system is formed and is made up of first spherical mirror 12, catoptron 13, second spherical mirror 14, the 3rd spherical mirror 17, the 4th diaphragm diaphragm 15, the 5th diaphragm 18, the 3rd photodetector 16, the 4th photodetector 19, collects incident optical axis direction big angle and little angle bidimensional optical information and is converted to electric signal.

Entire device is arranged on the seal closure, divides the four-dimension to be accepted with the closely-related light scattering information of leucocyte subclass rerum natura by the utility model device and is converted to electric signal, finally realizes the purpose of this utility model.

Claims (2)

1. a leucocyte subclass is analyzed multidimensional optical information sensor, and it is characterized in that: described sensor comprises laser instrument (1), first focus lens group (2), first diaphragm (3), double horn mouth streaming pipe (4), second diaphragm (5), first photodetector (6), second focus lens group (7), the 3rd diaphragm (8), hollow ellipsoid reflecting surface (9), polaroid (10), second photodetector (11), first spherical mirror (12), catoptron (13), second spherical mirror (14), the 4th diaphragm (15), the 3rd photodetector (16), the 3rd spherical mirror (17), the 5th diaphragm (18), the 4th photodetector (19), light trapping (20) and seal closure (21); First diaphragm (3), second diaphragm (5), the 3rd diaphragm (8), the 4th diaphragm (15) and the 5th diaphragm (18) are opened on the seal closure (21); Laser instrument (1), first focus lens group (2), first diaphragm (3), double horn mouth streaming pipe (4), light trapping (20), first spherical mirror (12), catoptron (13), second spherical mirror (14), the 4th diaphragm (15) and the 3rd photodetector (16) are positioned on the same incident light axis successively according to the direction of laser incident, and double horn mouth streaming pipe (4) is placed perpendicular to incident light axis and with a tight waist being centered close on the incident light axis; Second photodetector (11), polaroid (10), the 3rd diaphragm (8), second focus lens group (7), hollow ellipsoid reflecting surface (9), double horn mouth streaming pipe (4), second diaphragm (5) and first photodetector (6) are positioned on the same side scattered light axle, second focus lens group (7) is arranged in the vacancy of hollow ellipsoid reflecting surface (9), and lateral scattering optical axis and incident light axis intersect at the center with a tight waist of double horn mouth streaming pipe (4); Catoptron (13), the 3rd spherical mirror (17), the 5th diaphragm (18) and the 4th photodetector (19) are positioned on the forward scattering optical axis, and scattering optical axis and incident light axis intersect vertically; First spherical mirror (12) and second spherical mirror (14) constitute the 3rd condenser lens group, and catoptron (13) is positioned at the 3rd condenser lens group center, first spherical mirror (12) and the 3rd spherical mirror (17) and constitutes the 4th focus lens group.

2. a kind of leucocyte subclass as claimed in claim 1 is analyzed multidimensional optical information sensor, it is characterized in that: described double horn mouth streaming pipe (4) is made with quartz ampoule, the intermediate hollow pipeline structure is by mutual inverted double horn interruption-forming, velocity flow profile feature at the thin place of neck owing to the flow field can retrain particulate at the streamline middle part, energy counterflush during obstruction guarantees that stream normally uses.

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