CN1232824C - Urine grain stillage analysis integrated concentration and detecting analysis - Google Patents
Urine grain stillage analysis integrated concentration and detecting analysis Download PDFInfo
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- CN1232824C CN1232824C CN 200410022277 CN200410022277A CN1232824C CN 1232824 C CN1232824 C CN 1232824C CN 200410022277 CN200410022277 CN 200410022277 CN 200410022277 A CN200410022277 A CN 200410022277A CN 1232824 C CN1232824 C CN 1232824C
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Abstract
The present invention relates to a urinary sediment analyzing method integrating concentration and detection analysis, which comprises the steps: a test tube filled with urine to be detected is firstly placed in the sampling position of a urinary sediment analyzer; the urinary sediment analyzer automatically sucks the urine in a urine storage chamber whose back part is connected with a micro-porous filter concentrator, the urine is concentrated under the action of pressure difference through pressurizing the urine in the urine storage chamber and pumping vacuum on the other face of a filter membrane of the concentrator , and then the urine is automatically transferred into a counting cell/plate below a microscope when the specified concentration ratio is reached; images in different positions of the counting cell is automatically collected under the control of a scanning platform of the analyzer which obtains analysis results according to dry-chemical analytic results and the collected urinary sediment images for detection doctors to refer. The method utilizes the micro-porous filter membrane to concentrate the urine, replaces the concentration step in the original method, the concentration step can be directly realized on an instrument by a mature micro-porous filter membrane technology, and then the method achieves the purposes for simplifying operation procedures for doctors and enhancing detection speed and reliability.
Description
Technical field
The invention belongs to technical field of medical detection, be specifically related to a kind of urine sediment analysis methods concentrated, check and analysis that integrate.
Background technology
The urine sediment analysis method is divided into two classes at present: the first kind is to utilize the morphology inspection side method of light microscopic, and advantage is can detect and accurate all visible components of differentiating in the urine, is confirmatory test to urine sediment analysis, and the checkout equipment valency is low.The step of this method is as follows: the test tube that 1, urine to be detected will be housed is earlier put into hydro-extractor, 2, opened hydro-extractor centrifugal 5 minutes, 3, the test tube level is toppled over fall supernatant liquor, again test tube is placed on the suction sample station of urine sediments analyzer, 4, instrument adds urine sample in microscopically counting chamber or the tally, moves micro-platform, finishes the image acquisition of regulation area, 5, last doctor draws testing result according to the visible component in the image discriminating urine of gathering.Wherein employed urine sediments analyzer is existing matured product.By the said method step as seen, this method shortcoming is the step that exists many doctors of needs to operate in implementation procedure, causes the analytical instrument automaticity low, influences detection speed, and doctor's workload is many.The second alanysis method is to utilize Flow Cytometry and impedance bioelectrical measurement method, this method can detect red blood cell, leucocyte, epithelial cell, cast bacterium, speed is fast, but can not detect all visible components, the true picture of urine slag can not be provided, just scatter diagram, to urine sediment analysis, not confirmatory test, can only sieve, equipment valency height.
Examine the advantage of side method for the morphology that makes full use of light microscopic, the development automaticity can be compared with the instrument of impedance bioelectrical measurement method with utilizing Flow Cytometry, above-mentioned doctor manual operation section must be removed as far as possible,, increase the reliability that detects to improve detection speed.Several steps above analyzing, find the step that most importantly speed that influences concentrate with hydro-extractor, owing to be difficult for the hydro-extractor and the existing urine sediments analyzer that run up is integrated, cause this step to operate by the doctor separately, a urine sample concentrates and needs at least more than 5 minutes, and can not many parts of urine samples concentrate simultaneously, directly cause detection speed to be difficult to improve.Therefore, the method that must seek new concentrated urine substitutes hydro-extractor and reaches the concentrated urine purpose, does not destroy the composition that arena detects again, and easy and integrated speed and the robotization that improves urine sediment analysis of urine sediments analyzer.
Summary of the invention
The present invention is directed to the above-mentioned shortcoming that existing urine sedimentation detecting method exists, by liquid concentration analysis of technology with present maturation, the inventor has proposed a kind of urine sediment analysis methods concentrated, check and analysis that integrate, should come concentrated urine by miillpore filter, substitute the concentration step in the former method,, this step directly can be realized on instrument by the miillpore filter technology of maturation, reach and simplify doctor's operating performance, improve the purpose of detection speed and reliability.
A kind ofly integrate urine sediment analysis methods concentrated, check and analysis, the entire method step is as follows:
1. the test tube that urine to be detected will be housed is earlier put into the suction sample station of urine sediments analyzer.This step is different from the step of existing method, and existing method needs use the hydro-extractor concentrated urine earlier by the doctor in addition, outwell supernatant liquor after, the urine after will concentrating is again put into the suction sample station of urine sediments analyzer.
2. the suction needle of urine sediments analyzer sucks invisible spectro urine in the fluid storage compartment automatically, can finish the dyeing function to fluid storage compartment adding dye liquor by dye liquor application of sample mouth according to detecting needs.
3. be connected with the miillpore filter enrichment facility behind the fluid storage compartment, vacuumize by urine in fluid storage compartment pressurization with at the filter membrane another side of miillpore filter enrichment facility, urine is under differential pressure action, small-molecule substances such as moisture pass filter membrane and take out, the visible component that arena need detect is left and concentrates, behind the cocnentration factor that reaches regulation, urine is transferred in microscopically counting chamber/plate automatically.This step also is different from the step of existing method, existing method is directly transferred to concentrated urine in the counting chamber, or dyeing earlier, after the dilution concentrated urine is transferred in the counting chamber, it concentrates is not to finish in analytical instrument, but is gone to finish by doctor's manual operations with hydro-extractor in addition.
4, under the scanning platform control of analyser, automatically gather counting chamber diverse location image, analyser draws analysis result for check doctor reference according to the arena image of mummification analysis result and collection, and the check doctor confirms to import behind the corresponding information with regard to printable report.This step is identical with the step of existing method.
In the step 2, the pore size of filter membrane is to select suitable aperture for use according to the size of minimum visible component in the visible component to be measured in the urine.
This shows, this method is compared with existing method, it comes concentrated urine by miillpore filter, the principle of utilizing miillpore filter the composition greater than the filter membrane aperture in the liquid can be separated, adopt pressurization and vacuum filtration that small-molecule substances such as the moisture in the urine, salt are gone out, the film below stays the concentrated urine that contains whole visible components to be measured in the urine, reached the purpose of concentrated urine like this, substitute the concentration step that separates with analyser in the former method, to concentrate, check and analysis have combined in one, thereby have improved detection speed.And filtering with microporous membrane is ripe liquid concentration technology, it is close that it and human kidney produce urine, it is very little to the destruction that arena detects composition, be easy to the integrated urine sediments analyzer that advances, and it can make hyperchannel miillpore filter enrichment facility, can finish many parts of urines simultaneously manages outward, the test tube that the doctor only needs patient's urine will be housed is put into suction sample station, negative sample get final product after 2 minutes the result, exceptional sample adds that the doctor differentiates that the time manages outward, every patient can not surpass 4 minutes from sending urine sample here to reporting, and the doctor is simple to operate, and speed is fast.
Description of drawings
Fig. 1 is the synoptic diagram that this method realizes on urine sediments analyzer.
Embodiment
Fig. 1 is a kind of urine sediments analyzer of routine, and the miillpore filter enrichment facility is arranged on the fluid storage compartment liquid supplying passage afterwards of this instrument, and the concrete steps that this method realizes on this instrument are as follows:
(1) test tube that the 10ml urine that patient sends here will be housed is put into the suction sample rotating disk of urine sediments analyzer.
(2) suction needle of urine sediments analyzer sucks urine in the fluid storage compartment automatically, can finish the dyeing function to fluid storage compartment adding dye liquor by dye liquor application of sample mouth according to detecting needs.
(3) by with force (forcing) pump urine pressurization and connect vacuum pump and vacuumize leaching waste liquid outlet in fluid storage compartment, filter membrane upper and lower at the miillpore filter enrichment facility has formed certain pressure reduction like this, urine in the miillpore filter enrichment facility is under differential pressure action, small-molecule substances such as moisture pass filtering membrane and are taken out from leaching waste liquid outlet, the visible component that arena need detect is left and concentrates, detect the fluid storage compartment urine level by liquid level detection device, when liquid level drops to assigned position, stay the 200ul concentrated urine, allow urine seesaw urine is mixed, again urine is transferred in the microscopically counting chamber.(can not drop to the urine sample of assigned position to the upper limit liquid level that exceeds schedule time, illustrate that visible component is too many in the urine sample, liquid level detection device detects actual liquid level, and system calculates according to liquid level and stays volume of urine number/cocnentration factor, thereby has solved the function that part overrich urine sample need be diluted)
(4) scanning platform mixes up the microscope focal length and according to the rules scanning route moves counting chamber, counting chamber diverse location image is gathered automatically by microscope, camera and engine control system by system, instrument draws analysis result according to the arena image of mummification analysis result and collection, for check doctor reference, the check doctor confirms to import behind the corresponding information with regard to printable report.
The instrument of realizing this method can be on the market any arena detector that utilizes the morphology inspection side method of light microscopic at present, and the miillpore filter enrichment facility of routine is arranged at after its fluid storage compartment, becomes one with it at an easy rate.And the miillpore filter enrichment facility also can be a plurality of side by side, make hyperchannel miillpore filter enrichment facility, finish the outer reason of concentrating of many parts of urines simultaneously.
Claims (1)
1, a kind ofly integrate urine sediment analysis methods concentrated, check and analysis, it is characterized in that carrying out according to the following steps:
(1) the suction sample station that urine test tube that patient sends here is put into urine sediments analyzer will be housed;
(2) suction needle of urine sediments analyzer sucks urine in the fluid storage compartment automatically, finishes dyeing according to detecting needs adding dye liquor;
(3) suction needle of urine sediments analyzer sucks invisible spectro urine in the fluid storage compartment automatically, be connected with the miillpore filter enrichment facility behind the fluid storage compartment, vacuumize by urine in fluid storage compartment pressurization with at the filter membrane another side of miillpore filter enrichment facility, urine is under differential pressure action, small-molecule substances such as moisture pass filter membrane and take out, the visible component that arena need detect is left and concentrates, and behind the cocnentration factor that reaches regulation, urine is transferred in microscopically counting chamber/plate automatically;
(4) under the scanning platform control of analyser, automatically gather diverse location image in the counting chamber, analyser draws analysis result for check doctor reference according to the arena image of mummification analysis result and collection, and the check doctor confirms to import behind the corresponding information with regard to printable report.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410022277 CN1232824C (en) | 2004-04-10 | 2004-04-10 | Urine grain stillage analysis integrated concentration and detecting analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410022277 CN1232824C (en) | 2004-04-10 | 2004-04-10 | Urine grain stillage analysis integrated concentration and detecting analysis |
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| Publication Number | Publication Date |
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| CN1563988A CN1563988A (en) | 2005-01-12 |
| CN1232824C true CN1232824C (en) | 2005-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200410022277 Expired - Fee Related CN1232824C (en) | 2004-04-10 | 2004-04-10 | Urine grain stillage analysis integrated concentration and detecting analysis |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323281B (en) * | 2011-08-22 | 2013-09-18 | 爱威科技股份有限公司 | Liquid property detection method and system |
| CN103063633A (en) * | 2012-12-25 | 2013-04-24 | 南昌大学 | System capable of automatically detecting bacteria in water |
| CN103411882B (en) * | 2013-08-14 | 2015-11-18 | 彭玉洲 | A kind of method detecting urinary formed element |
| CN105445282B (en) * | 2014-08-22 | 2018-08-10 | 苏州惠生电子科技有限公司 | The recognition methods of counting chamber external dust, device and full automatic urine sediment analysis system |
| CN106053200B (en) * | 2016-06-29 | 2023-07-28 | 杭州龙鑫科技有限公司 | Urine enrichment device |
| CN106525857A (en) * | 2016-12-29 | 2017-03-22 | 山东朗伯光谱设备有限公司 | Method and device for testing chemical components and visible component of liquid |
| CN108318312A (en) * | 2017-01-16 | 2018-07-24 | 深圳市美侨医疗科技有限公司 | Urinary formed element staining analysis instrument |
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| CN1563988A (en) | 2005-01-12 |
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